LOCUS       DAXHTY010000001        84550 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXHTY010000001 DAXHTY010000000
VERSION     DAXHTY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 84550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84550
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.001"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(1293..1961)
                     /locus_tag="ACFNMK_000001"
     CDS             complement(1293..1961)
                     /locus_tag="ACFNMK_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6363357.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(1958..2626)
                     /gene="hda"
                     /locus_tag="ACFNMK_000002"
     CDS             complement(1958..2626)
                     /gene="hda"
                     /locus_tag="ACFNMK_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC6363358.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(2731..4402)
                     /locus_tag="ACFNMK_000003"
                     /pseudo
     CDS             complement(2731..4402)
                     /locus_tag="ACFNMK_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(4691..5338)
                     /locus_tag="ACFNMK_000004"
     CDS             complement(4691..5338)
                     /locus_tag="ACFNMK_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC6363359.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(5335..5703)
                     /locus_tag="ACFNMK_000005"
     CDS             complement(5335..5703)
                     /locus_tag="ACFNMK_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC6363360.1"
                     /translation="MNPKTLSCLSLCAAVLALTACGDNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(5713..6384)
                     /locus_tag="ACFNMK_000006"
     CDS             complement(5713..6384)
                     /locus_tag="ACFNMK_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC6363361.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(6549..7268)
                     /locus_tag="ACFNMK_000007"
     CDS             complement(6549..7268)
                     /locus_tag="ACFNMK_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6363362.1"
                     /translation="MATLTDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            complement(7352..8665)
                     /gene="hpnE"
                     /locus_tag="ACFNMK_000008"
     CDS             complement(7352..8665)
                     /gene="hpnE"
                     /locus_tag="ACFNMK_000008"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC6363363.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNPLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPYYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            complement(8744..9616)
                     /gene="hpnD"
                     /locus_tag="ACFNMK_000009"
     CDS             complement(8744..9616)
                     /gene="hpnD"
                     /locus_tag="ACFNMK_000009"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC6363364.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            9787..11649
                     /gene="hscA"
                     /locus_tag="ACFNMK_000010"
     CDS             9787..11649
                     /gene="hscA"
                     /locus_tag="ACFNMK_000010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC6363365.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCHLLEQNELSKLNEQDSRLLLSLVRSAKEK
                     LTAQTEAVIETTLSDGHKVHTVITRQEFHNLTQNLVQKTIEPVKQALKDAGVGKADVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTSRAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQVDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDSTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDGDLLEAEELQQI
                     QQDIADLQGRLKDGKAEDIRAAVAKLGSITDNFAAKRMNRNIQRALAGQSVDNI"
     gene            11725..12381
                     /locus_tag="ACFNMK_000011"
     CDS             11725..12381
                     /locus_tag="ACFNMK_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6363366.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPWLLAGAASYKPKKSK
                     IRLFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPQ
                     "
     gene            12418..13179
                     /locus_tag="ACFNMK_000012"
     CDS             12418..13179
                     /locus_tag="ACFNMK_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC6363367.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            13234..13575
                     /gene="fdx"
                     /locus_tag="ACFNMK_000013"
     CDS             13234..13575
                     /gene="fdx"
                     /locus_tag="ACFNMK_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC6363368.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(13675..14268)
                     /locus_tag="ACFNMK_000014"
     CDS             complement(13675..14268)
                     /locus_tag="ACFNMK_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFC6363369.1"
                     /translation="MNNALTAKLSKLVYTAPDNPDFLSKIGEFDRLILLVRRLYQMLD
                     EQNALSRLTLCIRCQNQRGLIVLDGARADSDAATIRMKRWHIYTQIAERMANRYDQIS
                     VTVENETANPTQLEQYYLDIADKTIALFGLKEAKLQQQEYPEDKTQQQSILKKLSEKP
                     GRNLSMINTKAKIEQYAANQTQTPLKITRQRFICRPN"
     gene            14345..14542
                     /gene="iscX"
                     /locus_tag="ACFNMK_000015"
     CDS             14345..14542
                     /gene="iscX"
                     /locus_tag="ACFNMK_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC6363370.1"
                     /translation="MKWTDTQRIAEDLYDLHGETIDPKTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(14610..15011)
                     /locus_tag="ACFNMK_000016"
     CDS             complement(14610..15011)
                     /locus_tag="ACFNMK_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6363371.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            15222..16181
                     /locus_tag="ACFNMK_000017"
     CDS             15222..16181
                     /locus_tag="ACFNMK_000017"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC6363372.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            16249..17559
                     /gene="tilS"
                     /locus_tag="ACFNMK_000018"
     CDS             16249..17559
                     /gene="tilS"
                     /locus_tag="ACFNMK_000018"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC6363373.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCEEGSFDTVRWQAFSPRRKTHILRYFLKENGIPMP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPFHPDFPILF"
     gene            17633..18418
                     /locus_tag="ACFNMK_000019"
     CDS             17633..18418
                     /locus_tag="ACFNMK_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC6363374.1"
                     /translation="MRHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEGKMPSEEIRRLRAVVPEGRIFSVSDGILKKISSLSCADDILALIDI
                     PLGGTLPDKGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLSGIYPQADLEIWLARYKGRVFATALREEKQAVLYGEDLCGPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            18428..18625
                     /locus_tag="ACFNMK_000020"
     CDS             18428..18625
                     /locus_tag="ACFNMK_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363375.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            18887..19240
                     /locus_tag="ACFNMK_000021"
     CDS             18887..19240
                     /locus_tag="ACFNMK_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363376.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            19252..19557
                     /locus_tag="ACFNMK_000022"
     CDS             19252..19557
                     /locus_tag="ACFNMK_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363377.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            19619..20995
                     /gene="mpl"
                     /locus_tag="ACFNMK_000023"
     CDS             19619..20995
                     /gene="mpl"
                     /locus_tag="ACFNMK_000023"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC6363378.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFANLGAIQTQFHHL
                     VRTVPSEGLIVCNGQQQSLQDTLDKGCWTPVEKFGTEHGWQVGEVNADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSAFKNVKRRMEIKGTVNGI
                     TVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKAALPESLKGADQ
                     VFCYAGGVDWNVAEALAPLGGKLHVGKDFDAFIAEIVKHTEAGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            21202..22254
                     /gene="bioB"
                     /locus_tag="ACFNMK_000024"
     CDS             21202..22254
                     /gene="bioB"
                     /locus_tag="ACFNMK_000024"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC6363379.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(22401..22967)
                     /locus_tag="ACFNMK_000025"
     CDS             complement(22401..22967)
                     /locus_tag="ACFNMK_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6363380.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGSHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKIDPEKPLSGGLFI"
     gene            complement(23091..23330)
                     /locus_tag="ACFNMK_000026"
     CDS             complement(23091..23330)
                     /locus_tag="ACFNMK_000026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363381.1"
                     /translation="MGVCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVRRILSVHIAGI"
     gene            23317..23550
                     /locus_tag="ACFNMK_000027"
     CDS             23317..23550
                     /locus_tag="ACFNMK_000027"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6363382.1"
                     /translation="MQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHSNDFGAQGIT
                     ATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(23613..25472)
                     /gene="ilvD"
                     /locus_tag="ACFNMK_000028"
     CDS             complement(23613..25472)
                     /gene="ilvD"
                     /locus_tag="ACFNMK_000028"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC6363383.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRNIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(25645..27414)
                     /gene="cysI"
                     /locus_tag="ACFNMK_000029"
     CDS             complement(25645..27414)
                     /gene="cysI"
                     /locus_tag="ACFNMK_000029"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFC6363384.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGEKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILSELDDLIGKWAAGRDTDEGFGDFAIRTGI
                     VKPVLDAPVDFWDASKAVAIARA"
     gene            complement(27441..29255)
                     /locus_tag="ACFNMK_000030"
     CDS             complement(27441..29255)
                     /locus_tag="ACFNMK_000030"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HFC6363385.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQATPPAGLQTASEGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(29252..30538)
                     /locus_tag="ACFNMK_000031"
     CDS             complement(29252..30538)
                     /locus_tag="ACFNMK_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HFC6363386.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDAHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEVTLC
                     WFDERPLNTARKYLLKHGTQTVSAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(30577..31500)
                     /gene="cysD"
                     /locus_tag="ACFNMK_000032"
     CDS             complement(30577..31500)
                     /gene="cysD"
                     /locus_tag="ACFNMK_000032"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFC6363387.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(31538..32272)
                     /locus_tag="ACFNMK_000033"
     CDS             complement(31538..32272)
                     /locus_tag="ACFNMK_000033"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HFC6363388.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRFRP
                     VREDADRYVENKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(32288..33748)
                     /gene="cysG"
                     /locus_tag="ACFNMK_000034"
     CDS             complement(32288..33748)
                     /gene="cysG"
                     /locus_tag="ACFNMK_000034"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFC6363389.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGD
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELEVLARHHIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGTLKAALI
                     AERLQQYGRPPDTPAAVISHGTQSTQSTAIGTLANLARLAENAPKPALIVVGEVVELH
                     EKLAWFGENAKKESNPAEHAYFALDGLGTGQEQQAA"
     gene            complement(34198..34854)
                     /locus_tag="ACFNMK_000035"
     CDS             complement(34198..34854)
                     /locus_tag="ACFNMK_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC6363390.1"
                     /translation="MKNKSKVWDFPTRLSHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALAAAAILLLS
                     "
     gene            34919..35809
                     /locus_tag="ACFNMK_000036"
     CDS             34919..35809
                     /locus_tag="ACFNMK_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC6363391.1"
                     /translation="MFPVFHLSGESRRRMLQAASRFPYVFKARAEDSHKGTFGTLAVV
                     GGAVGMSGAPVLAASAAMYLGCGKVWAGFNQETLPFAVIAGFPEIMLDTADGLTKRQD
                     INAWVVGCGLGTGRAAVRTLAGILAEHTDKPVLLDADALNILSTDAETRNLARECKNL
                     ILTPDPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
     gene            36079..37890
                     /gene="typA"
                     /locus_tag="ACFNMK_000037"
     CDS             36079..37890
                     /gene="typA"
                     /locus_tag="ACFNMK_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC6363392.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQVVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(38440..38530)
                     /locus_tag="ACFNMK_000038"
     tRNA            complement(38440..38530)
                     /locus_tag="ACFNMK_000038"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38494..38496),aa:Ser,seq:gga)
     gene            complement(38569..38655)
                     /locus_tag="ACFNMK_000039"
     tRNA            complement(38569..38655)
                     /locus_tag="ACFNMK_000039"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38619..38621),aa:Leu,seq:cag)
     gene            complement(38691..38777)
                     /locus_tag="ACFNMK_000040"
     tRNA            complement(38691..38777)
                     /locus_tag="ACFNMK_000040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38741..38743),aa:Leu,seq:cag)
     gene            38866..41241
                     /gene="rnr"
                     /locus_tag="ACFNMK_000041"
     CDS             38866..41241
                     /gene="rnr"
                     /locus_tag="ACFNMK_000041"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC6363393.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKSDLVKCRIEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPHVERLCVVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIEHPFKTQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQL
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKAKSSASVKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            41350..41505
                     /locus_tag="ACFNMK_000042"
     CDS             41350..41505
                     /locus_tag="ACFNMK_000042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363394.1"
                     /translation="MKDCVYFHRRFEGRRYSADGAQTAFRRHFHSFGVSVRIALPVRA
                     ISLCGLP"
     gene            complement(41580..43043)
                     /gene="guaB"
                     /locus_tag="ACFNMK_000043"
     CDS             complement(41580..43043)
                     /gene="guaB"
                     /locus_tag="ACFNMK_000043"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC6363395.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(43244..43324)
                     /locus_tag="ACFNMK_000044"
                     /pseudo
     CDS             complement(43244..43324)
                     /locus_tag="ACFNMK_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(43370..43831)
                     /locus_tag="ACFNMK_000045"
     CDS             complement(43370..43831)
                     /locus_tag="ACFNMK_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363396.1"
                     /translation="MNIIMDSTRKFGILWEENSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNAVFNSLKSSFEEKYYAGGNNGLDFGEQLFDIDKYNSLELCNIFSIDTTYMSG
                     GGNCEIDCLVLEMGYSGEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(43922..46480)
                     /gene="glnD"
                     /locus_tag="ACFNMK_000046"
     CDS             complement(43922..46480)
                     /gene="glnD"
                     /locus_tag="ACFNMK_000046"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC6363397.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGGIGELCESVRDDITGDTAFLEARFLFGNRQTADELAKKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSTVAQKEDIQDPSVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSNSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSLDLKNPKIQSSLKQTLLEQLS"
     gene            complement(46529..46849)
                     /locus_tag="ACFNMK_000047"
     CDS             complement(46529..46849)
                     /locus_tag="ACFNMK_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6363398.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            complement(47174..47647)
                     /gene="bfr"
                     /locus_tag="ACFNMK_000048"
     CDS             complement(47174..47647)
                     /gene="bfr"
                     /locus_tag="ACFNMK_000048"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6363399.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLEKEKEKHEALI
                     AAISVAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(47675..48139)
                     /gene="bfr"
                     /locus_tag="ACFNMK_000049"
     CDS             complement(47675..48139)
                     /gene="bfr"
                     /locus_tag="ACFNMK_000049"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6363400.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(48602..49585)
                     /gene="lipA"
                     /locus_tag="ACFNMK_000050"
     CDS             complement(48602..49585)
                     /gene="lipA"
                     /locus_tag="ACFNMK_000050"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC6363401.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(49578..50201)
                     /gene="lipB"
                     /locus_tag="ACFNMK_000051"
     CDS             complement(49578..50201)
                     /gene="lipB"
                     /locus_tag="ACFNMK_000051"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC6363402.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(50203..50478)
                     /locus_tag="ACFNMK_000052"
     CDS             complement(50203..50478)
                     /locus_tag="ACFNMK_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC6363403.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            50663..51733
                     /locus_tag="ACFNMK_000053"
     CDS             50663..51733
                     /locus_tag="ACFNMK_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC6363404.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(51840..52787)
                     /locus_tag="ACFNMK_000054"
     CDS             complement(51840..52787)
                     /locus_tag="ACFNMK_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC6363405.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(52802..53209)
                     /locus_tag="ACFNMK_000055"
     CDS             complement(52802..53209)
                     /locus_tag="ACFNMK_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC6363406.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            53290..53949
                     /gene="ung"
                     /locus_tag="ACFNMK_000056"
     CDS             53290..53949
                     /gene="ung"
                     /locus_tag="ACFNMK_000056"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC6363407.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            54097..54864
                     /locus_tag="ACFNMK_000057"
     CDS             54097..54864
                     /locus_tag="ACFNMK_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC6363408.1"
                     /translation="MRAFIKNYVLTLLFLKYVSDKHKYGGGMIELHADTTFDDIVKLK
                     NTADIGDRLNKIIAQIAEANDLKGVIDVADFNDEDKLGKGKEMIDRLSRLVGIFEKLN
                     LSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRIMAKIIGISADCPSST
                     SVYDPTCGSGSLLLKAAAQAGSQISLYGQEKDVATASLARMNMILHNNETAEINTGNT
                     LSESTAEKLKTCKISPPIFMAAYLRTIWTHWKPIGKF"
     gene            55058..56002
                     /locus_tag="ACFNMK_000058"
     CDS             55058..56002
                     /locus_tag="ACFNMK_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6363409.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEDANLTV
                     VFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            56153..56278
                     /locus_tag="ACFNMK_000059"
     CDS             56153..56278
                     /locus_tag="ACFNMK_000059"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363410.1"
                     /translation="MLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQTYNLT"
     gene            complement(56568..57083)
                     /locus_tag="ACFNMK_000060"
     CDS             complement(56568..57083)
                     /locus_tag="ACFNMK_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363411.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(57549..59471)
                     /locus_tag="ACFNMK_000061"
     CDS             complement(57549..59471)
                     /locus_tag="ACFNMK_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6363412.1"
                     /translation="MIEIKNLTLQRGLKILLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALLKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQVQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFAGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(59548..59919)
                     /locus_tag="ACFNMK_000062"
     CDS             complement(59548..59919)
                     /locus_tag="ACFNMK_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC6363413.1"
                     /translation="MLFEENPIDGHFTEYGHRSGEIRLAGQSFHKPVLVHKDSVCLSQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            60116..61423
                     /locus_tag="ACFNMK_000063"
     CDS             60116..61423
                     /locus_tag="ACFNMK_000063"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC6363414.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            61416..61850
                     /locus_tag="ACFNMK_000064"
     CDS             61416..61850
                     /locus_tag="ACFNMK_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363415.1"
                     /translation="MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(61909..62178)
                     /locus_tag="ACFNMK_000065"
     CDS             complement(61909..62178)
                     /locus_tag="ACFNMK_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC6363416.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(62360..64822)
                     /gene="lon"
                     /locus_tag="ACFNMK_000066"
     CDS             complement(62360..64822)
                     /gene="lon"
                     /locus_tag="ACFNMK_000066"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC6363417.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLTQRQQILEISEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKGIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            64922..65098
                     /locus_tag="ACFNMK_000067"
     CDS             64922..65098
                     /locus_tag="ACFNMK_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HFC6363418.1"
                     /translation="MPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVLPPYFQ
                     NMTQVFHVFFYFSR"
     gene            65073..66176
                     /locus_tag="ACFNMK_000068"
     CDS             65073..66176
                     /locus_tag="ACFNMK_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6363419.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     SSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQADLRVADVL
                     KTKPVHKKAADPDDPLAFARNRETTFLYRLKKA"
     gene            complement(66248..67993)
                     /gene="recD"
                     /locus_tag="ACFNMK_000069"
     CDS             complement(66248..67993)
                     /gene="recD"
                     /locus_tag="ACFNMK_000069"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC6363420.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDATGARQNLAKWFQGAGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPDFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(68061..68756)
                     /gene="lolD"
                     /locus_tag="ACFNMK_000070"
     CDS             complement(68061..68756)
                     /gene="lolD"
                     /locus_tag="ACFNMK_000070"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC6363421.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAETAMSMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFGRVMVM
                     KDGSLHPKQGANA"
     gene            complement(68749..69996)
                     /locus_tag="ACFNMK_000071"
     CDS             complement(68749..69996)
                     /locus_tag="ACFNMK_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC6363422.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            70201..70479
                     /locus_tag="ACFNMK_000072"
     CDS             70201..70479
                     /locus_tag="ACFNMK_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363423.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSDSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(70535..71152)
                     /gene="recR"
                     /locus_tag="ACFNMK_000073"
     CDS             complement(70535..71152)
                     /gene="recR"
                     /locus_tag="ACFNMK_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC6363424.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVDHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(71219..72757)
                     /locus_tag="ACFNMK_000074"
     CDS             complement(71219..72757)
                     /locus_tag="ACFNMK_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC6363425.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPTNEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYIRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(72817..73191)
                     /locus_tag="ACFNMK_000075"
     CDS             complement(72817..73191)
                     /locus_tag="ACFNMK_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC6363426.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQMPSEAEICS
                     WLEQVPLETLLNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            73342..74970
                     /locus_tag="ACFNMK_000076"
     CDS             73342..74970
                     /locus_tag="ACFNMK_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC6363427.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANLEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNATMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGTIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            75042..76295
                     /locus_tag="ACFNMK_000077"
     CDS             75042..76295
                     /locus_tag="ACFNMK_000077"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC6363428.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYGFEIAEETMKLMRQM
                     VANGEADALVAERVWQELAKGLMEKNPRKMIEVLRECGALKVLLPEVDALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PMREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
     gene            76518..76826
                     /locus_tag="ACFNMK_000078"
     CDS             76518..76826
                     /locus_tag="ACFNMK_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363429.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            76908..77939
                     /gene="ruvB"
                     /locus_tag="ACFNMK_000079"
     CDS             76908..77939
                     /gene="ruvB"
                     /locus_tag="ACFNMK_000079"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC6363430.1"
                     /translation="MLQTDNLTAAQPQRIITAQSISSQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(77962..78690)
                     /gene="rpe"
                     /locus_tag="ACFNMK_000080"
     CDS             complement(77962..78690)
                     /gene="rpe"
                     /locus_tag="ACFNMK_000080"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC6363431.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            78836..79270
                     /locus_tag="ACFNMK_000081"
     CDS             78836..79270
                     /locus_tag="ACFNMK_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC6363432.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(79283..80146)
                     /locus_tag="ACFNMK_000082"
     CDS             complement(79283..80146)
                     /locus_tag="ACFNMK_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6363433.1"
                     /translation="MELNEFFDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(80154..80771)
                     /locus_tag="ACFNMK_000083"
     CDS             complement(80154..80771)
                     /locus_tag="ACFNMK_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC6363434.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(80903..81508)
                     /locus_tag="ACFNMK_000084"
                     /pseudo
     CDS             complement(80903..81508)
                     /locus_tag="ACFNMK_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
     gene            81714..83486
                     /locus_tag="ACFNMK_000085"
     CDS             81714..83486
                     /locus_tag="ACFNMK_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HFC6363435.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAVENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            83483..84139
                     /locus_tag="ACFNMK_000086"
     CDS             83483..84139
                     /locus_tag="ACFNMK_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6363436.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
BASE COUNT        19140 a        21982 c        23015 g        20413 t
ORIGIN      
        1 gtcgccttgt cttgattttt gttaatccac tatagaaaca aaaacagaag cctaagatcc
       61 gtcattcccg cgcaggcggg aatctagacc tgtcggcacg gaaacttatc gggaaaaaag
      121 gtttctttag attttatagt ggattaacaa aaacctgtac ggcgttgcct cgccttagct
      181 caaagagaac gattctctaa ggtgctgaag caccaagtga atcggttccg tactatttgt
      241 actgtctgcg gcttggtcgc cttgtcctga tttttgttaa tccactatac gtcctagatt
      301 cccactttcg tgggaatgac gggatgtggg tttttgtgcg gatttgaacc ggtaagggtg
      361 gtgtgggatt ggtggtttgc ttaggatctt ttggattgta gtttgtatat acatttactt
      421 gttgataaaa gataaaataa aattagaaac taaaagtgag aaaaaattaa taataatagg
      481 gatgtataaa tgtaaaggct ccgtttcata gctaaggtta tctgaatata tggaaaaaaa
      541 gtaaaagtcc ataaaactaa aatatataga taatgctaat gataatagaa ttatcacttt
      601 cctagagtaa ggataatatt tttttatcgt aaaaaaatat aaaataggat aaataattgc
      661 catatagata gattttgtaa caaaattaaa aataaataat aaaaataatg taataaagta
      721 tattttttgg ctatgaaata aaattgtaca taattgaacg agcagatcaa aaaatgaact
      781 acatataaca ataaataata acgtatttac catactaaat ttaataggtc tcattatcat
      841 atttaataac cacttcatag tatagtggat taaatttaaa ccagtacagc gttgcctcgc
      901 cttgccgtac tatctgtact gtctgcggct ttgtcgcctt gtcctgattt ttgttaatcc
      961 actataaatg cagagtgggt ggaaacactc actttatggt ttgctacgct ctgctcaatt
     1021 agcaacccga taacccaata tggataatag ggtaattaat ccaatctaat ttgtcagcat
     1081 ccgttaattt attgcaaaat aaagtattga attatgtcgg gtgcaaatga cgaaatataa
     1141 gtttccgtgc ggacggatca agattcccac tttcgtggga atgacggtgg aaagattgtt
     1201 gtttttccgg ataaattcct gtggctttga gtttttggga tttcagcccc aatgccgtct
     1261 gaacgccgaa tcgggcttca gacggcattg cgtcatttga aattcaaaac cggccagcct
     1321 ttttctttgg cttctttttc cagctcggca tcggggttga cggcgacggg ttcgtcgacg
     1381 aggcgcagca gcggcaggtc gtttttggag tcgctgtaaa aataggtttt gccgtagctt
     1441 tcgagcgttt cgccgcgttc ggcgagccat tggttcaggc gggtgatttt accttctttg
     1501 aggctgggcg tgccgatgta attgccggtg tagcggccgt cggaaccgga ttcgagttgt
     1561 gtgccgatga tgttggtaat gccgaaaagg cggcagacgg gggtgatgat gaactcgttg
     1621 gtcgatgaga tgacgagggt ttcgtcgcct gccatttggt ggctctgcac cagcatacgc
     1681 tgcataggcg agatgtgggg gatgatgtat tccgccataa attcgcggtg aaactctgcc
     1741 agctcttctt tgctgtaacg agcgagcggg gcaaggtgga atttgaggaa tgcgtcgatg
     1801 tcgaggcaac cgttttggta gtcgcggtag aatttttcgt tttgcgcttc ggtttcggca
     1861 gcgtcaacca agcctttttt gatgaggtat tgcggccagg cgtggtcgga atcggtgttg
     1921 atgagggtgt tgtcgaggtc gaagatggcg aggtttttca ttgggtttcc tgttgtttca
     1981 aaagctggcg caaaagcggc agggtgatgc gtttgcccat tgtgacggcg tagttgtcca
     2041 gcgtgtcgag catcatcatc aggctgtcca tatcgcgccg ccagtgtttg agcaggtatt
     2101 cgaaaatttc ggaatcgacg gttacttggc gtgccgccgc catactggcg agcgcgtcga
     2161 ttttttcttg gtcggttaag ggtttgactt cgtaaacgag gcagtacgcc atacgcgtcc
     2221 gcaaatcttc gcggatgaca agctgctggg gcgtgtattc cgaaccgagc agcaaaaagc
     2281 ctttgccgct gttgcggaag cggttgaaga tggaaaaaag cagggcttgt tcttcgttgc
     2341 ccagtttttc gacttgatcg acggcgaggt attccgcctc gaacgcggca tcggtcagcg
     2401 gcatggaggc ggcatcgata taggcggcgt ttttgccggc ttcgagcgcc tgtgcgaccc
     2461 acgcctgcaa aagatggctt ttgcccgcgc cttcttcacc ccagacatag ataaactgtc
     2521 cgtgtttgtg tcggaggaca tagaccagtt ccgcgttttc cgtgccgagg aatttgtcga
     2581 aactcggata gtcgtgtgcg gcaaagtcga aaataagctg attcacggtt cggcattccg
     2641 aggggtggta aacgggttta ttgtacgttg ttttcgcgcg cctttccaat ttgaacgatg
     2701 ccgtctgaaa acggcttcag acggcatcgt tcaaccgcag gcaacgttgc cgacatcgag
     2761 gcgcatattg tggaacgcgt tgagcgtgct tcggtgtccg atgctgatga tgatgctgtc
     2821 gggcagtttt tgctttaagg cgcggtagag cgcggcttcg gccggttcgt ccaaggcggc
     2881 ggtggcttcg tcgagcagga cgattttggg cttggaaagc agggcgcgga cgaaggcgac
     2941 gcgttgcagt tcgcccaggg agagtttgtg ctgccagtcg tctgttttgt ccaatttgtc
     3001 aatcagataa cccaagcggc aggtgttcat ggcttcgatt agctccggat gctgtttgtc
     3061 gatgtcggga taacaaaccg cgtcgcgcag gctgccctgt gccgtgtacg ggcgttgcgg
     3121 caggaagagg atgtcttgat gcggcggacg gctgactttg ccgttgctgc cgaacggcca
     3181 aagcccagcc agcacgcgca gcagcgaggt tttgccgcaa ccgctcggac cgcgtatcag
     3241 cagggaatca ccgcttttga ggttgatatt gataccgtct aataaaactt cgccgttgtg
     3301 gtggaacaga gcgacgtttt ccagtgcaat gccgtctgaa acttcgctaa tctcgggttg
     3361 ccgcgcgctg tgttgttctt cggtactcag caaaaagccg tacagacgct ccaatcgggc
     3421 gcggtaggcg gtgaatttgt tgtagaacat tcgaaagaaa gaaagcgcgt tttgcagtcg
     3481 cgcgaaggct tggacggttt gctggatgtc gccgattttg atttgtccgg caaacaggcg
     3541 cggagcttgc aaaataatcg ggaagagttt gatgccgttg gtgaacatat cgttaaagcc
     3601 gcttaagcag acgctttgcc gcgcaatgcg ccaacggttg cggataatgg ctttaaagcg
     3661 gtcggaaagc tggtcgtatt catgtttttc gccgttgtaa aacgccacgc tttcggcgtg
     3721 gtcgcgcacg cggatgaggg aataacggta gtcgccgttg agtttttcgt tttcataatt
     3781 gtaacgaatc aaagggttgc ctatccacat ggcgataaag gtcgccaaaa tcacaaatat
     3841 atagacaaac caaacgatgc cgtgcggaat gtcgaagccg aacacggtca ggatgccagc
     3901 caagccccgc aaaacaacgg caaattccag agaagtaacg accgaattga ccatgccgcg
     3961 cacaaattcg atggtcgaag cgataaattc ctgcgcgtcc tgttggatac gctggtcgat
     4021 gttgtctggc gcgtggcggc gcatttgcag gcggtagtag tttttgtcgg caagccagcg
     4081 tgctgtcagc acttcgttga gccgctccga ccatttaatc gccaagcctt gatcgaggaa
     4141 gtcgttgacg acgttgttaa acgcccgtat cagcaccacg cccgcgttca ttgcagcaaa
     4201 catccaaaat gccgaagcat ttcaaatcct gcatcgagtc gtaaagccct ttggacataa
     4261 aggtactcaa cacattcagc cgcatttcgg ttaacaccag cgtaatcatc gccgtaatca
     4321 gcagcaagac tttgaccgcg cttttcagtg tcagacaaag ccaaagcggt gtggaataaa
     4381 gctcggtttg ccatttctgc atgggaaatt tcttacggta tcaatgccgt ctgaaaaaga
     4441 cgggtacagt tgattttttg atgaagtttg gggaagtttt gcaggtcagg gcatattgcg
     4501 tgttaattta ccatacaacc acgccggaat taagtttaaa tttgaataaa aggttcgggt
     4561 tctgcaaaat acagaacccg aaccttgttc ggatattgaa accggccgcc cgattttggg
     4621 cggtgcggct tgcaagtatc aagattcgca tatgccgtct gaagctcgga gaggttcaga
     4681 cggcatatgc ttatttgggc tgctcttcga cgaatctcgg acctttcaag atgccgttgt
     4741 gagaataggg cgacagcagg ttgtatgcgg cggttttgga aacctgataa ccgcggtcgg
     4801 tcaggctgtt ggcaatctga ttgaccactg cgctgaccaa agcccccaac aggccgctgt
     4861 tgctgttgtt gctgccttcg cggatgctgg ccgaacccga ccacaactct tttccgttgc
     4921 gggaatcgac cagccgtgct ttggcggata cggtcgtcac gctgtctaaa atttgatatg
     4981 aagtgccgta ttcggtaacc gtaatgtaca aaaccgcatc attgccgaaa atctgatgca
     5041 gtttttccgg ccggacggcg tgaatatcgg cggcattggt caagccgttt tgtttgaagg
     5101 tttcctccac gactgcggcg gggaagacgt aatagccggc ttcggaaagc ggcgcggcgg
     5161 tcgaagccag tacaccccat gttccgttga catcgggcga ttcgttcagc ggcggaacca
     5221 ccaaaattga agccggtttg ctttccttga atgacgtgta gtcgaaatcg ggagcttttt
     5281 gaacttggca ggcagacagc gccaacacgg cggcaagccc taaaatcaaa ggtttcatcg
     5341 cttgcctcct ttaccggttt tcatcaggaa gtccataaat acgcccgatt cgggaaacag
     5401 ccttttctct tcttcaaact ggcggaacgc gccctctttg tctcccgaac gggaaagcag
     5461 cagtcccaga tgggcgtgcg cacccggggc ggcattcatt tttttgttgc cggcttccac
     5521 aaagtatttt tccatctttt cggtctgctt gcccaacgaa gtgtcgtcgt ttttcaaacc
     5581 ttcatagacg gtatcgggat agccgccgta ataatacagg gatttttgcc cgttgtcgcc
     5641 gcaggcggtc agagccaaga ccgccgcaca cagcgacaaa cagctcaagg ttttcggatt
     5701 catcatttct ccttaacggt tgggttgcca tgcgccgttg tcaacagcct gaaccaggct
     5761 gttgacggct tcgcggattg ccaagtctaa aactttgccg ttcaaagtcg catcgtagcc
     5821 ggaagtgccg ccgaaaccga tgatttcacg gttggaaagt gcgtattcgc ccgcgccctg
     5881 tgcggaatag acgatttcgg aagtattgac gttgacgata ttcagagcca cttttgcata
     5941 ggcgatttgc gatttgccgc gacccaaaat gccgaagagc tgatgatcgc cgacatctct
     6001 gcgtccgaat tcggttacat cgccggtaac gacataatct gcgcctttca ggttatgcgc
     6061 tttgccggaa atgccggatt cctgttttaa tgcgttcaaa ttggtgcggt tcagtacgtt
     6121 gaagcggttg gtctgttgca ggtgcgttac cagaatggtt tttgcctggc tgcccaaacg
     6181 gtcttccccg tcggagaaaa tgcctttttg gaagctggag cggttgtcga atgttccgac
     6241 ggaaatcggg gtacgaacac cgtgatattg cgtattgtag gaggcgactt tctctacctc
     6301 gagactgcgt gaggattcgg tcgcacagcc ggtcagtgaa acggcagcgg cggcaaggac
     6361 aacggcggtg gaaacggttt tcataaaatt taccctaagg tcaagttaag gaaataacgg
     6421 gttgtcatta ttgtccttat gtaaatttaa gtcaaggtgt ttgtctgtgc gggacggatg
     6481 cgcgcggaag gtacggatat ttttcaaacg gaatcgcggc gcgggcggga tgcctgcctt
     6541 ttccggattt agaggtaaac gatttcgcca ctccgccctt tgctttcggt acttgcccac
     6601 cagacaaatg cgggcagcac gtccccgtag cttttgcgtt cgcttttggc ttcgcccgga
     6661 tgggatttga tgcgttgcgg ggaattgatg gggccgggga cgaggacgtt ggcgcgcagg
     6721 ttgccgaagc gttcccattc gtcggcggcg actttgcaca ggtagttcaa cgcggctttg
     6781 gacgcgccga agccgcccca gtaggctttg ggtgtttcgc cgtggctttc gccgacgaag
     6841 atgacggacg cgtcgggcga ctgcttcagc agcgggaaca gggcgcgggt cagccccata
     6901 ggtgcgacgg tgttgatgcg gtattggttg acccattcgg cgacggtttg gaaatccagc
     6961 ggcgagaggg cgtaaaaata gccggcgcag tggacgatgc cgtccagttt gccttgcgtg
     7021 gcttcggcaa tggtggcggc gaactgattg aattcttttt cttccgcgct aataaggtca
     7081 aagcagatgg cgaatggttc ggggtatccg gcttcgacaa tcgcgtcata cactttttcc
     7141 agttttttct gatgacgggc aaccaaaatc acagtcgcgc ctgctgccgc ataggctttg
     7201 gcaacctgtt cgcccagccc ttgcgatgcg ccggtaacta agatggtttt gtcggtcagt
     7261 gtcgccatac tttttccttt ttggtggtcg gtcgggggta ttttagcgtt ttgccgcacc
     7321 ttgtaaagcg tcctgatgcc ggccggcgtt ttcagacggc atcgttcagg ctttgcaggc
     7381 aggcttccgc ggaagcgaaa cctgattgta cggcggcttc gagcgtggcg gggtagtatg
     7441 ggtggaggta gtcgccggcg gggaagatgc ggtgccggtg cagccacgac aaatccggtg
     7501 gtggggcatc ggctgcggtt gtggcgcgtt tttcggtgat gacgcgcacg gcttcgggtt
     7561 cgcccaaatg cggaaggatg cgtttgaggt cggcgtgggc tttgtccgcc cacgcccggt
     7621 ttgcaaacgc gccgacgcgg tcggaaacgc tgatgacggc ggacacttcg ttttcaggca
     7681 gtccgagcct gccccggcaa agcagccatt gcaccgtgcc gtcggcaagg ccggtcagcg
     7741 gggcgggcag gcggacgggt tcggcgtagc gcagatagac ggtggtgatg gcgtgatagc
     7801 gaaggttttg atatgccgtc tgaacgtgtt cgggcgtgcc ttcgggcagg agcgcggcgg
     7861 cgtggtaggg cgcggtggcg gggacggcgg catcgaaagc ttcgccgttg acgagcactt
     7921 tcccgtccgg gagggggttc agacggcata cgcgcgtttc gaggcggatg tccgcgccga
     7981 gccgttgaag ctccgccaag gcgggttcgg cgacgattgc gcccaaatcc tgcttgggta
     8041 ggagatagtc gctgccggat tttttcgtca gcacgccgtc ggacaaaacg ttgcacaaca
     8101 cgcgcaggct tgcggtttcc aaaggcgtgt tgagcgcacc ccacaccagc ggctgccaaa
     8161 actgcatcac ggcggcacgc ggcacgttcc gctgtttcag ccattgtgca actgtcgtgt
     8221 cgggctgtcc gaggcgtgcg gacttctgca aatcggacat atcggcaagc agtttggctt
     8281 tgaatgcagt cggtgcacgc cgggcaagca gcacgccgcc caaaatatgc agcggcgcgg
     8341 gcagggggag ggcgcggaac tgcaaaccgc cgtgcatatg ccagtgcagc ggtacgcgca
     8401 aaaaggcggc acggggatcc gaaccgatgg ttttcatcag gcgcaacacg ccccggtatg
     8461 cgccgagcaa aatgtgctgc ccgttgtcca aaaaaccgaa accgtcggta tttccggcca
     8521 gtgtgcgcgc cctgccgccc gcctgccggc cggcttcaaa cagggtaacg tcggcgtgcc
     8581 gcgccaaggt gacggcggcg gacagtcctg cccagcctgc gccgatgacg gcgattttcg
     8641 ggcgcggatg cggcgtgttc atcatttatt cctccaatgg ttgaaacccc gtttttcggg
     8701 gcggcggggg ttgggttttc ctgaagggcg ggcggtatgc gccttatgcc cgttccggcg
     8761 tgccggggcg cggtttgaat ccgaataacc aggttttcag ggcaatgcgt tttttgcgcg
     8821 gcgaagggag ggcgattttg tatttgagga cgttttgcgc gccgtcccgg tcgatttcgt
     8881 tcaatagctc gtaataaacc gccgccataa ccagtccgac tttttgggct tttttatcgg
     8941 cagccggcag cagcgatacg gcttcgcggt aggtttcacg ggcgcgtttg atttggaacg
     9001 ccatcaattc ggcaaaattg cccgtcgggc tgcattgcaa aatcacgctt gcggggacat
     9061 caaaccgctg catttcctcc atcggcaggt aaatccgtcc cctgcgcgcg tcttcgccga
     9121 catcgcggat gatgttggtc agttgcagcg caagtcccat cttgtcggcg tattccagcg
     9181 ttttgccgtc tgaaaacccc aaaatccgcg caatcaggca gccgactaca cctgcaacac
     9241 ggcggcagta cagtttcaat tcttcaaaac tgccgtaacg cgcccgaacc aaatccatct
     9301 gcatcccgtc gattaaggct tccagttcat atttcggcag cttgaaggtt tccttaacct
     9361 gccgcaaggc ctgattgacg gggtgttccg gcatcgcgcc gccgaatacc ttgtccaaat
     9421 cgccgcgcca ccagttcaat gtcgcctgtg caacatcggg gttggaacat tcgtcaacca
     9481 catcgtccaa ttcgcggcaa aaagcatata aaaccgttac cgcatcccgt ttttcctgag
     9541 tcaggaaacg gaagcccgac aaaaaactgg agcggctttc ttctgctttt tggcggcaat
     9601 agtcgagtcc tttcacgatt tatattccta atgatgggag ggaaaggcgg attttatcgg
     9661 catttggcgg tagagggcaa tttcggcggc acgacccaat ccttagcggt ttgctcaact
     9721 atcggcgcga attctgttaa aatgccgact tccttccttt acacaccgca ccgacaggca
     9781 gaatttatgg ctcttttgca gatttcagaa ccgggtatgt ccgccgcccc gcaccgacac
     9841 cgtttggcgg caggcatcga tttgggtacg accaacagct tggtcgccac cgtccgcagc
     9901 ggcagtgccg cctgtctgcc cgatgccgaa gggcgcgtta ccctgccttc cgtcgtccgc
     9961 tatctggaaa acggcggcat tgaagtcggc aaaaccgccc tgtccgccca aaaaaccgac
    10021 ccgctgaaca ccgtcagctc cgccaaacgc cttatcgggc ggacgcttgc cgatctgcat
    10081 caaaatacgc attacctgcc ttaccgtttc ggtgacaatc aacgcgttat cgaactgcat
    10141 acgcggcagg gggtgaaaac gcctgtcgaa gtgtcggcgg aaatcctcaa aacccttaaa
    10201 tcgcgcgccg aagaaacctt gggcggcgat ttggtcggcg tggtgattac cgtccccgcc
    10261 tatttcgacg atgcccaacg ccaggccacc aaagatgccg cgcgtctggc gggtttgaac
    10321 gtattgcgcc tgctcaacga acccaccgcc gccgcaatcg cctacgggct ggacaacgcc
    10381 tcggaaggca cgtttgtcgt gtacgactta gggggcggca cattcgacgt atccgtattg
    10441 caactgacca aaggactgtt tgaagtcaaa gccaccggcg gcaacagcgc gttgggcggc
    10501 gacgacttcg accatcgttt gttctgccac ctgctcgaac aaaacgagct ctccaaactc
    10561 aacgagcaag acagccggct gctgctttcc cttgtccgta gtgccaaaga aaaactgacc
    10621 gctcaaaccg aagccgtcat cgaaaccaca ctttcagacg gccataaagt tcataccgtc
    10681 attacccgcc aagagtttca caatctgacc caaaatctgg tacaaaaaac catcgagccg
    10741 gtcaaacagg ctttgaaaga tgcgggcgtg ggcaaggcgg atgtcaaggg cgtgattatg
    10801 gtcggcggtt cgacccgtat gctgcacgtc caacaagcag ttgccacatt ctttggtcaa
    10861 accccgctga acaacctcaa tcctgacgaa gtggtcgcgc tcggcgcagc catacaggca
    10921 aacgtcctcg ccggcaacaa agccgacggc gaatggctgc tgctggacgt tactcccctg
    10981 tcgctcggtt tggaaaccta cggcgggctt gccgaaaaaa tcatcccgcg caattccacc
    11041 atccccacct cgcgcgcgca ggacttcacc acgttcaaag acggtcagac cgcgatgacg
    11101 atacacgtcg tacaaggcga acgcgaactg gtttccgact gccgcagcct tgccaaattt
    11161 accttacgcg gcattccgcc gatggcagca ggcgcagcgc gtatccgcgt aaccttccaa
    11221 gttgacgctg acggattact atccgtttcc gcacaagaac aaagcaccgg cgtacaggcg
    11281 caaatcgaag tcaaaccttc ctacggcttg gacgacagca ctatcaccca aatgctcaaa
    11341 gacagcatga gcaacgctgc cgaagacatg gccgcacgcg cacgcgccga agccgtagtc
    11401 gaagccgaaa gcctgaccga tgccgtcaac gccgcccttg aattggatgg cgatttgttg
    11461 gaagccgaag aattgcaaca aattcaacaa gacatcgccg atttgcaagg ccgtctgaaa
    11521 gacggcaaag ccgaagacat ccgcgccgcc gtcgccaaac tcggcagcat taccgacaac
    11581 ttcgccgcca agcgcatgaa ccgcaacatc caacgtgcgc tggcggggca aagcgttgat
    11641 aatatttgat ggcaaatggt ttcagacggc acagatataa cctgatgccg tctgaaggct
    11701 cgaaaacact tgaaaaacat cgatatggaa aagtcaggca ttgtctatct gatgaaaacc
    11761 gtcatcaagg gcgtgtataa aatcggcatt tcggacgtaa gcaattttga aggcagaatg
    11821 cgccatttgg aaaacaacgg ctatgcgaac gttgccggat tggaacgcat cctcgccgtc
    11881 aaaaccgaca attacaaaga aaaagaaaac ctgctccatg aaattttcag caaaagcagg
    11941 ataggcgata ccgaattgtt cgccgtggac gaaaaccttg taaaacgttt gtttttatcg
    12001 cttcgcggcg aaatcgtgtt tccgaaaaac gaaacggcgg aatcggaatt tgaaaaaagc
    12061 gtccacgaac gcaggcagga agggaatgcc gggtccggcc gcaaacaact gcttgatttg
    12121 gtacggcgcg gacaccggga atacccttac gcgctgccct ggcttttggc gggcgcggca
    12181 tcctacaagc cgaaaaaatc gaaaatccgc ctttttaaag aagcatattt cggcaaaagc
    12241 ggcacgaggc tgaccgacga aattgcagac ggcatccata tttacacctg tttttcgcgg
    12301 gcggatttgg aaaaagccta ttccgaatat ttggaacttt tcaaatccga atcggatgcc
    12361 gaaggcagaa agccgcagta aggtgcaaac agataccgta cacgttgagg agcagatatg
    12421 atgggcgatt ccgtcattta ttatgtagaa caggcagaca aaccggtaaa ccgtgccggc
    12481 gaacgcgccc gtaaaacatt caaatatttt tggcgcgagc ttttttggga acgccgccgc
    12541 atcattcccg ccttagattt tgccatggtc aaagcccctt ttttccaaga cggcgaagac
    12601 ggcgaaattt gcgaacatat gtggatcgac gatatttatt ttgacggtct ttacatttat
    12661 ggcgtgctga acaatgaacc cggagaactg accaatgtcg aacaaggcga aagcgtttgc
    12721 gttccggttg acgacatcag cgactggatg ttcgtgtgca acggcattcc ctacggcggc
    12781 tttaccatac aggcaatgcg tggacagatg acggaagagg agcgtaccga acacgatgcc
    12841 gcatggggaa tcgatttcgg cgatcccggg caggtattgc tggtgtatga agaaaaagaa
    12901 catcccgaaa atctggaaga gcatccgatg tgccggaact gtattgacga ttttcggcaa
    12961 cagttgtccc aaaatccgga ttttctgcat gagcaggacg aagacggcta tacgccgctt
    13021 catcatgaag ccatcgcagg aaatgcactt atggttcaag ccatgcttga atacggcgca
    13081 aatcctgcct caaagacatc ggaaggctat accgccctcg attttgcccg cctggcgggc
    13141 tggcaaaatg ttgccgacct gctcgaaccg cgacattagg cggacagttt tccgaaaacg
    13201 aacacaaaca ctttttacag aaagacaata aaaatgccca aaatcaccgt acttccacac
    13261 acgacattat gccccgaagg cgcggttatc gaagacgcgc ccgaaggcaa aaccgtcctc
    13321 gacgtgctgc tcgaccacga catcgaagtc gatcacgcct gcgaaaaatc ctgcgcctgc
    13381 accacctgcc acgtcatcat ccgcaaaggt ttcgacagtt tggaagagcc gaccgaattg
    13441 gaagaagacc tgctcgatca ggcttggggt ttggaagccg attcgcgcct gagttgtcag
    13501 gcggttgtcg ccggcgaaga tttgattgtg gaaatcccca aatatactat caatcatgcc
    13561 cgagaggagc attgacgcaa tgccgtctga aacatcatgc ggttcagacg gcatcggata
    13621 tgcataagcc ggagcagaag ggtaatcccg tatttgataa gggtaaaccg gagtttagtt
    13681 tggacggcag ataaaccgtt gtcgggtaat tttcagaggt gtttgtgtct gatttgccgc
    13741 atattgctct attttggctt tcgtattgat catactcagg tttcgaccgg gcttttcgga
    13801 tagctttttc aatattgatt gctgctgcgt tttatcttcc ggatattctt gctgttgcag
    13861 tttcgcttcc ttcaatccaa aaagagcgat agttttatcg gcaatatcca gatagtattg
    13921 ttcaagttgg gtaggattgg cagtctcatt ttcaactgtt acggaaatct ggtcataacg
    13981 gtttgccata cgttcggcaa tttgggtata aatatgccac cgcttcatac gtattgttgc
    14041 ggcatcggaa tcagcccgtg caccgtctag gacaataagc cctctttggt tttgacagcg
    14101 gatgcaaagt gtcagccggg aaagggcatt ttgttcgtcg agcatctgat acagtctgcg
    14161 gaccaatagg atcagacggt caaattcccc tatcttggaa aggaaatcag gattgtccgg
    14221 agcggtataa accagtttgg aaagtttggc ggttaaggca ttattcattc agctatcctt
    14281 ggcgggtata catttggaac atgttctata cggttgaaag accggaaaat acaggaaaat
    14341 taatatgaaa tggacagaca cacaacgtat cgctgaagac ctttacgacc tgcacggcga
    14401 aaccatcgat cccaaaaccg tgcgctttac ccaactgcgc gacctgatta tggcattgcc
    14461 cgaatttgac gacgaccccg cccgttgcgg cgaacgtatt ctcgaagccg tgcagcaggc
    14521 atggatagac gaggcagaat aagtttcggg aatgccgtct gaaatacggc ggtacgcggt
    14581 tcgtgcttct gtttgcagtg ggaacggttt taccagtctc cttttttcag cctatccagt
    14641 tggcggcggt cgcgcttggt cggtctgccg tcgggatggg cggaagtgat gcggctgaat
    14701 tggtcgagct gtttgcgctc ttccctcaat gctgccgttt ttgcgtcttc ttcatacaga
    14761 agccgcgcct cggatgccgg gcggcgttgg tggttcaaac ctttaacctt gattttatag
    14821 ggaagggaat tgagcgtcag gtcgataata tcgccgatgt ctatggtttt actgtttttg
    14881 accttcgagc cgtttacttg aaccctaccc agttcgatgt gcttttgcgc aagggaacgg
    14941 gtcttgaaaa aacgtgccgc ccaaagccat ttgtccagcc gcatggcgga agaatcgtgc
    15001 ttgtctttca tacgattttg tttgaaataa ttgaatttgt ttcaagttta gcataagata
    15061 cgccgcctat aactagtata tatgcactaa tccactgttt tccatgctgt ccgaacacaa
    15121 aaagagggtg tggaaaagcc gttttggaca ataaattaac tgcggaatat gcacaaatag
    15181 cgtatgatag cggcagaatc tgttgatgag agcttcattc tatgaaacct gtttttttgg
    15241 attttgaaca acccatagcc gaactgacca acaaaatcga tgagctgcgt ttcgtccaag
    15301 acgagtctgc cgtcgatatt tcggacgaaa tacaccgttt gcagaaaaaa agcaacgacc
    15361 tgaccaaatc gattttcagc aaacttacgc ccgcccaggt ttcacaggtt tcccggcatc
    15421 cgcagcgtcc ctatactttg gattacattg atgcgctgtt tactgatttt gaagagctgc
    15481 acggcgaccg ccactttgcc gacgatcatg cgattgtcgg cggattggcg cgtttcaacg
    15541 gacaaagcgt gatggtcgtc gggcatcaga aagggcgcga caccaaagaa aaaatccgcc
    15601 gcaacttcgg tatgccccgt cctgaaggct accgcaaagc cctgcgcctg atgaagacgg
    15661 cagaaaaatt cggcttgccc gtcatgacct ttatcgatac gccgggcgcg tatcccggca
    15721 tcggcgcgga agaacgcggg cagtcggaag ccatcggcaa aaacctgtac gaactgacgc
    15781 gcctgcacgt ccccgttttg tgtaccgtca tcggcgaagg gggctcgggc ggtgcgttgg
    15841 cggtcgccgt aggcgattac gtcaatatgc tgcaatactc gacctattct gttatctccc
    15901 ccgaaggctg cgcttcgatt ttgtggaaaa ccgccgaaaa ggcggcggat gcggctcagg
    15961 ctttgggcat tactgctgac cgcctgcaaa agctggactt ggtcgatacc gtcatcaaag
    16021 aaccattggg cggcgcgcat cgggattttg ggcaaaccat gaaaaacgtg aaagccgttt
    16081 tggaaaaaca actgcacgaa gcgcaaagca tcccgcttgc cgatttgctt tcgcgccgtt
    16141 tcgaccgcat tatggcttac ggcaaatttt cggaacaata attcaggtag aacaagcagc
    16201 aagcagtttg tctgaaactg cttgcttttt ctttatcggg acggaaccgt gctgacttta
    16261 gatgcgtttg agcaatgctt gaaggattgt tttcctcaag gtctgaatgg aaaaaaaaca
    16321 gcggtggcat taagcggcgg cttggattcc gtcgttttgc tgcatctgct tgtccgcgcc
    16381 ggaaaaaagg gcggttttat tccggatgca ttgcatatcc atcacggctt gagtccccgt
    16441 gccgacgatt gggcagattt ctgccaaaac tattgcgata tgctcggggt ggggctggaa
    16501 acggttaagg tctgcgtgga aaaaaacggt ttgggcatcg aggcggcggc gcggcaaaag
    16561 cgttatgccg cgtttgccga aaaaggcttt gacgttttgg cgttggcgca ccacagggac
    16621 gatcaaatcg aaacctttat gctggcagtc gctcgcggcg gcggtttgcg cgctttggcg
    16681 gctatgccca ccgtccgccc ttttggggaa aaaggcatca tctggcggcc tttgctgcct
    16741 ttttcgcgcc aagacatatg ggattatgcc caaaaacacg gtttgccgaa tatcgaggac
    16801 gaaagcaata gcgatacggc ttatttgcga aaccgcttcc ggcaccgtat tttgcccgaa
    16861 ctttcggcgc agattcccca tttcgggcgg catgtactga acaatgtccg cgctttgcag
    16921 gaagatttgg ctttgttgga cgaaatcatc ggtcaggact gccgttgggt ctgtgaggaa
    16981 ggcagtttcg atacggtgcg ttggcaggcg ttttccccac gccggaaaac ccatattttg
    17041 cggtattttt tgaaggaaaa cggcatcccc atgccgaatc agaatgccct tgccgacatt
    17101 gcccgggttt tgacggaggc aaaaaccgga cgttggaact tgcaaggctt tgaattgcat
    17161 cattatgcag gcaggctgtt tgtgttccga ctggaaaaaa cggataaact gcggtttttg
    17221 aaagacaggc agataagcgg aaatttaagg gaaatattga cggggcaggg atttgtttta
    17281 aaacgtcatc cgtttgggct tcctgagcat cttttggagc aggacggaat tttgaggacg
    17341 gtagcggtat cggatacgtt ggctgtcggc ggcatccata aggatgtgaa aaaaatcctt
    17401 caggggaaac gggttttgcc tgtcctgcgc ccaatttggc cgcttgttgc cgacagcgga
    17461 aaccgtccat tggcgttggc aaactgttgt gcggattttc aaatctcggt ttcagacggc
    17521 attttgccct tccatcctga ctttcccatt ttattttgat aatatcgcaa acagatttcg
    17581 gcggcgttca gtcgggtatt gtccggttgc atatttctaa aaggcttgtg aagtgagaca
    17641 catcagttcg accaataatg aacacatccg gcacctgcac cgcctgttgt cgcaaggaaa
    17701 gttcagacgg caatacgccc aaaccgtttt ggagggcgtg cacctgcttc aggttttcct
    17761 gcaatccggc gggatgccgg tcggggtata tattcccgag ggcaaaatgc cgtctgaaga
    17821 aatccgcagg ctgagggcgg ttgtgccgga aggcaggatt ttttccgttt cagacggcat
    17881 attgaaaaaa atcagtagct tgagttgtgc agacgatata cttgcactga ttgatatccc
    17941 attgggcgga accttgccgg acaaaggcga ctgtgtggtt ttggacggcg tgcaagatcc
    18001 gggcaatgtc ggcaccgtct tacggagtgc ggcggcggca ggggtaggta cggtcgtttt
    18061 gggcaggggt tgcgcggacg cgtggtcgcc caaggtattg cgcgcgggga tgggcgcgca
    18121 tttcttgtcg ggcatttatc cgcaggcgga tttggaaata tggttggcgc gctataaagg
    18181 ccgtgtgttt gccaccgcct tgcgcgagga aaagcaggcg gttttgtacg gcgaagattt
    18241 gtgcggaccg acagcctggg tgtttggcaa cgaaggcgcg ggggtcggta aagcggtttt
    18301 ggacagggcg gacaagtgtg tcaggattcc gatgcacgat gcaaccgagt ctttaaatgt
    18361 cgcgatggcg gcgacaatct gcctgtttga acagatgcgg cagcggtcgg cgtattgagg
    18421 aaaagaaatg ccgtctgaaa aaatctatta cggcgtattg attttcttat gtatcgcttc
    18481 tatgctgctg tcgccgtttt tttatgcggg tgctttgaag cccaagaagg cggcattgcg
    18541 gaaggacggg cagtggaaac tcatcttatt gtccaatacc gtggcggcgg cggttttggc
    18601 ttgggtgtgg tggaaatggt tttgacagat attgctcaaa atcgtgctaa tggaatccga
    18661 acaaataaag aatttggtaa aaaatttgtt aaatcaacga attaaagttt tgtggaaaac
    18721 aaaacagctc taagcaaata gggcgtttgt cggtaaatac ggaagagttg cggcattatc
    18781 gggcatcttt aacaagtagt gccgtcttgg caggcaatcg gtttttatgg gcagcttgca
    18841 aaatcgcgga tataaaattg cgaatcggtt aaagtgtggg gacgctatga aaaattgcga
    18901 atttttttat gacccgacaa gggcaatcta tgatagcggt gcagattact taactaggga
    18961 aaaacataga ttagtcgtaa ttgcaaatag tgcttggggg ctattactta atttatcttg
    19021 ttattatgac gaggttttgg aaaagcggaa aataccgttc ggcaaacagg aaattgatga
    19081 cgatatggac aaagtgtccg cccttaagcg gaagtttaaa gatatttctg aaatcaaagt
    19141 aggggatggt tgggaatacc cgttcaatta tgagcaggga atgaaagaat tagatgaagt
    19201 gctattgaaa tacattccct tttttgaaga agaacgataa aggaggttga tatgcgcgta
    19261 tctaaaataa ttggaagtat gttgcttgtt acagcggttc agaccgtatt ttcggcaaat
    19321 gtttacgcgt gccgccataa tggtaaaacc agttacagcc aaactccggg aaaacattgt
    19381 accaacgcgg gtttggggcg ggaccgggtg tacagttcgg ttagaccggc agtaaaagac
    19441 agggcggaag acgcaggggt cggcgattat tcggacacgg tgagggacga acacgtccaa
    19501 aatccgagag agaatacgca taaggatgct tcgaataccg gtgcaaagac gcattgattg
    19561 aaaccgaatc agccatgacg gcgttgaata atagaatttg aacgattagg gaactctgat
    19621 gaaacacatc cacattatcg gtatcggcgg cacgtttatg ggtgggattg ccgccattgc
    19681 caaagaagca gggtttgaag tcagcggttg cgatgcgaag atgtatccgc cgatgagcac
    19741 ccagctcgaa gccttgggca taggcgtaca cgaaggcttc gacgcggcgc agttggagga
    19801 atttaaagcc gacgtttacg tcatcggcaa tgtcgccaag cgcgggatgg atgtggttga
    19861 ggcgattttg aaccgcgggc tgccttatat ttccggtccg caatggctgg ctgaaaacgt
    19921 gttgcaccat cattgggtac tcggcgtggc ggggacgcac ggcaaaacga ccaccgcgtc
    19981 tatgctcgcg tgggttttgg aatatgccgg actcgcgccg ggcttcctca tcggcggcgt
    20041 accggaaaat ttcggcgttt ccgcccgcct gccgcaaacg ccgcgtcaag acccgaacag
    20101 caaatcgccg tttttcgtca tcgaagccga cgaatacgac actgcctttt tcgacaaacg
    20161 ctccaaattc gtgcattacc gcccgcgtac cgccgtgttg aacaatctgg aattcgacca
    20221 cgccgacatc tttgccaact tgggtgcgat acagacccag ttccaccacc ttgtacgcac
    20281 cgtgccgtct gaaggcttaa tcgtctgcaa cggacagcag caaagcctgc aagacacttt
    20341 ggacaaaggc tgctggacgc cggtggaaaa attcggcacc gaacacggct ggcaggtcgg
    20401 cgaagtcaat gccgacggct cgttcgacgt attacttgac ggcaaaaaag ccggacacgt
    20461 cgcttggagt ttgatgggcg gacacaaccg catgaacgcg cttgccgtca tcgccgccgc
    20521 acgtcatgcc ggagtcgata ttcagaccgc ctgcgaagcc ttgagcgcgt ttaaaaacgt
    20581 caaacgccgc atggaaatta aaggtacggt aaacggcatc accgtttacg acgatttcgc
    20641 ccaccatccg accgccatcg aaaccaccat cgaaggcctt cgtcagcgcg tcggaaacgc
    20701 ccgcattctc gccgttctcg agccgcgttc caacaccatg aaactcggca ccatgaaagc
    20761 cgccctgccc gaaagcctca aaggtgccga ccaagtgttc tgctacgccg gcggcgtgga
    20821 ctggaacgtc gccgaagccc tcgcgccttt gggcggcaag ctgcacgtcg gcaaagattt
    20881 cgatgctttc attgccgaaa tcgtgaaaca taccgaagca ggcgaccata ttttggtgat
    20941 gagcaacggc ggtttcggcg gaatacacac caaactgctg gacgctttga gatagcccgg
    21001 gcgatgccgt ctgaaagccc ttcagacggc atcgtccggc tgcgcggcac aaaggcggaa
    21061 aaaccgtttg ccccgtattt tcaaacgcgt tacacttgcc gccgctgttt tcagccattt
    21121 gattacccgc aaccgccgtc attgcgccgg cggtttgcct gtcagcgtca ttgcgccgct
    21181 gtaaatacga aagaacacat tatgaccgta tcccccgtcg ccttgcgccg taagaccgag
    21241 tgcaagcctc atcccaccgc gcgctattgg aaaaaatgcg atgtcgaagc cctgttcgga
    21301 cttcccttcc tcgacctcat ttaccaagcc gccgaaatcc accgccaaaa tttcaacccg
    21361 cgcgaaatcc agctttccac gctgttgtcc atcaaaaccg gcggttgtcc cgaagattgc
    21421 gcctactgtc cgcaatcggc gcaccacaac accaatctgg gcaaagagca gatgatggat
    21481 gtggatgaaa tcgtcgaaaa agccaaaatc gccaaatcgc gcggcgcaag ccggttttgt
    21541 atgggcgcgg catggcgcgg ccccaaaccc aaagacgtgg agacggtttc cgcaatcatc
    21601 aaagccgtca agggcttggg tatggaaacc tgtggcacgt tcggtatgct cgaagaaggt
    21661 atggcggaag acttgaaaga ggcgggcttg gattattaca accacaacct cgacaccgac
    21721 cccgaccgct acaacgacat catccataca cgccggcatg aagaccgtat ggacaccttg
    21781 ggcaaagtcc gcaacgccgg tttgaaagtc tgctgcggcg gcatcgtcgg gatgaacgaa
    21841 acccgcgccg aacgcgcagg gctgattgcc agcctcgcca acctcgaccc gcagcccgaa
    21901 agcgtgccga ttaaccggtt ggtcaaagtg gaaggtacgc cgcttgccga tgccgaggat
    21961 ttggactgga cggaatttgt ccgcaccatc gccgtggcgc ggattacgat gccgcaaagt
    22021 tatgtccgac tgtcggcagg gcgcagcaat atgccggaag cgatgcaggc gatgtgcttt
    22081 atggcgggcg cgaactcgat tttttacggc gacaagctgc tgaccacggg caatccggat
    22141 gaagacggcg acagaatcct gatggaaaag ctcaacctgt atcccttaca gtttgagctg
    22201 gaaggcgagg tcgccgaagt ggaaaaagcc tctgggatta aagtggatta ttgacgattg
    22261 caaaaatgcc gtctgaaacc cgaaaaaagg ctttcagacg acatttgttc ggacggtatt
    22321 tccaatatct ttttaccggc gcgtgatgtt gccgtcgggc gagacatcca gcccgttccc
    22381 gttggggaag tcgctgcggt tcagataaat aatccgcccg ataacggttt ttcagggtcg
    22441 attttgggga ttttatcgtc ggcttgggta atagggatga tagttccgga aataaattta
    22501 ccctgtttgt cggtgataat tttgaaaatc ggcgcaatgc cgctgatgcc ggaggtgttg
    22561 attgcgccgt aggtggcaaa gttgccgccg ctgtaggaga tgaagcggtc gcggtaaagt
    22621 tcgacggcgc gagtaacgtg cgacccctgc ccgaatacga catccgcgcc ggaatcgacg
    22681 gcaagccgtg caaactcgac gacgttgccc ctgttttccc catagaagat ttcggtatcg
    22741 aacggcaggt gttccgcctg tttcccttcc gcgccgccgt ggaacatcac aatgacgatg
    22801 tcggctttct gtttggtttt ggtaatccgt tttctaactt tggcataatc gttcagtttg
    22861 acggcggcaa ggttgggggc gaaggagacg aagccggatc ttacgccgtt tttcttcagg
    22921 atggcggttt caaacctgtt ttcgatgccc gaatatttga tgttcaattc gtcaaggttc
    22981 gccctttatg ccgtttccgc accgcaaacc gccggggtca agccctcggc gcatcctgcc
    23041 ggaacggaat ccccgtgctg ccgattgacg gttcaaaccc cgcgcccgtt tcaaataccg
    23101 gcgatgtgga cggacaggat gcgcctgacg aaaagacagc cgataccgtt tccattatcg
    23161 gcgtgggcga cattatgctc ggcagcaatt atccggtcga ttacctgccc gataccaata
    23221 ttctgaaaaa cgtcgaatct gccttgcaag acgcggacat taccgtcggc aacctcgaag
    23281 gcacgctgtt tgacgaaggc ggtacgccga aaaaatgtgc aaacccccaa aatatgctat
    23341 gcattccgaa cgccctccgc atacgggcaa taccttgccg acgcgggatt cgactacctc
    23401 agcttcgcca acaaccacag caacgacttc ggcgcgcaag gcattacggc aacggcggcg
    23461 agcggcagct cttttacata ctcgatcgcg ctaaagccgc tgccgataac gaggccaaaa
    23521 ttgcggaaaa taccgccatc gcccagataa atttgtccat catcagacct ttactgttca
    23581 gacgagacag catttgccgc acgttttggg gcttatcttt cgatttgcgc tacgtcgcgc
    23641 accgcgcctt tgtcggcgga agtcgccatc gcgccgtaag ctcttaatgc ggcggagacg
    23701 tagcggtcgc ggttttccgg tttccacgct ttgctgccgc gtgcttccat ttcggcacgg
    23761 cgcgcggcaa gctcttcatc ggaaatgaca aggtggatgc tgcggttggg gatgtcgatt
    23821 tcgacggtat cgccttcgtg taccaaaccg atcgcgccac cttccgccgc ttcgggcgag
    23881 gcgtgtccga tggacaaacc tgatgtgccg ccggagaagc gtccgtcggt taagagagcg
    23941 caggctttgc cgaggccttt agatttcagg taggaagtcg gatacagcat ttcctgcatg
    24001 cccgggccgc ctttcgggcc ttcgtagcgg atgatgacga tgtcgccagc gacgatttgg
    24061 ttgcccaaaa tgccttcgac agcggcttcc tggctttcaa acacgcgcgc gcggccggta
    24121 aatttgagga tgctctcgtc cacgcctgcg gtttttacca cgcagccgcg ttcggcgatg
    24181 ttgccgaaca agaccgccaa accgccgtct tgcgagtagg cgtgtgccac gtcgcggata
    24241 cagccttttt cgcggtcgag gtcgagggtt ttccacatac ggttttgcga gaacgcttgg
    24301 gtggtgcgta cgccgccggg cgcggctttg aagcgttcga tggcgcgggt gttttcaggg
    24361 ttggttacgt cccatttttc aatcgcgtct ttcagcgtcg gtgcgtggat agtgtgcacg
    24421 tcggtgtgca gcttgcccgc tttgtccaat tctttcagga tggcgaagat gccgccggct
    24481 cggtgcacgt cttccatata gtagtcgtgg ttgttgggtg cggttttgca gatgcagggc
    24541 acaacgcggc ttaagcggtc aatgtcggcc attttgaaat cgacaccggc ttcgttggca
    24601 acggccaaca ggtgcaaaat ggtattggtg ctgccgccca tcgcaatatc catcgtcata
    24661 gcgttttcaa acgctttttt ggtggcaatg ttgcgcggca gtacggtttc atcgttttgc
    24721 tcgtaatagc gtttggtgat ttcgacaatc atacggccgg cttcgaggaa caattctttg
    24781 cggccggcgt gggtcgccaa atacgaaccg ttgccgggca gggaaaggcc gagtgcttcg
    24841 gtcaggcagt tcatcgagtt tgccgtaaac atacccgaac acgagccgca ggtcgggcag
    24901 gcgttttgtt cgacttcctc gacttgccgg ttgctgacat tgtcgtccgc cgattcaatc
    24961 atcgcgtcaa tcaagtccaa gcggcgttcg ggttggatgt ttgccacgcc gatgactttg
    25021 cctgcttcca tcgggccgcc ggaaacgaag atggtcggga tattcaggcg catggctgca
    25081 ataagcattc ccggggtgat tttgtcgcag ttggaaatgc acaccagcgc gtcggcgcag
    25141 tgggcgttga ccatatattc gatggagtcg gcaatcaaat cgcggctggg cagggagtag
    25201 agcataccgc tgtgtcccat agcgatgccg tcgtcgatgg cgatggtgtt gaattctttg
    25261 gcgattgcgc cggctttttc gatttcgcgg gcaaccagct ggcccatatt gtgcaggtgg
    25321 acatggccgg gcacgaattg ggtgaaggag ttggcaacgg cgatgatggg cttgccgaag
    25381 tcggtttcca tcacgccggt ggcgcgccac aatgcacgtg cgcccgccat attgcggccg
    25441 tgggtggagg ttttggagcg gtattctggc atagtgtgtt tccttgtgcc tataccgtct
    25501 gaaagacagg gctgtttcag acggtatcgg gtacggtttt ttagagtggg aaaagagggt
    25561 attttatacc aagtatcgga attttgcggg attgaaacgg cgtgcggcaa aaaagaaaat
    25621 ccccgcagga atgcggggac gggttcaggc gcgggcaatc gcgacggctt tggatgcgtc
    25681 ccaaaaatca acgggcgcat ccaacacggg tttgacgatg cccgtccgta tggcgaaatc
    25741 gccgaaacct tcgtcggtat cgcgtcccgc cgcccatttg ccgatcaggt cgtctaattc
    25801 ggaaaggatt tccggcaggg taatgttttc tttgtagaga cgggggatgc gtacgccttc
    25861 gcggtcgccg ccgatgtgga ggttgtagcg tccgacggct ttgccgacca gtccgatttc
    25921 cgccaacatc gctcgtccgc agccgttcgg gcagccggta atgcgggtaa cgatgtagtc
    25981 gtccgacgtg ccgtgtttcg ccataatctt atccagctcg ccgatgaagt ccggcagcac
    26041 gcgttcggct tccgccattg ccagcgggca ggtcggaaag gaaacgcagg acatcgcatt
    26101 ttcacgcagt ttactgacat cgttgcggat taatccgtat gttcgggcaa gttcttcgat
    26161 ttttgctttg tctgcttcgg cgacatttgc cacgatgagg ttttggttgg cggtgatgcg
    26221 gaaatcgcct ttgtggattt tggcgatttc caacacgccg gtcagcagct gtttctcgcc
    26281 ttcgtcaacc aaacgcccgc tttcgatgaa aagggtcaaa tgccagttgc cgtctatgcc
    26341 tttcacccag ccgatgcggt cgccgcgccc ggtaaatttg aacgggcgta tgggttcgaa
    26401 cggcataccc atacggcgtt cgacttccgc gcggaagttg tccaagccca tattttgaat
    26461 ggtgtagcgg gtgcgggcgt ttttgcggtc gctgcggttg ccgaagtcgc gctgcgtggt
    26521 taccaccgct tcggcggcct tcagcgcgtg ttccggaggc acgaaaccca gttccagtga
    26581 aatgttcgga taggttttgg tgttgccgtg ttccatcgaa agcccgccgc ctgccaaaac
    26641 attgaagccg gcaagctgtc cgttaccgtc tgaaacggcg acgaaatcca aatcgttgcc
    26701 gtagcagtcc acatcgttca agggcgggat gacgactgcg gttttgaatt ttcgcggcag
    26761 ataggttttg cccaaaatcg gctcgtcttc ttgaaggaag tcgtcggaac tttgaacttt
    26821 tttgccgtcc acccacacat ccagataacc gcgcgtgcgc ggcagcaagt gttcggaaat
    26881 ctttttcgcg tattcgtaag cctgccggtg cagttcggac tcgatcgggt tggacgtgca
    26941 aagcacgttg cggttcatat ccgccgccgt ggcgatggaa tccaaaccca gtttgtgcaa
    27001 gaggcggtgc atcgtctgca acttggcttt cggcacgccg tgaaattgga aggtttgccg
    27061 gttggtcagc cggatggagc ggtaatgact gttttcccgg gcaaatttgt ccagttctat
    27121 ccattgggac ggtttgatga tcccgcccgg cagccggcag cgcaaaagca taaatttcaa
    27181 gggctcaagt tttgcctcgg cgcgttcggc gcggatgtcg cggtcgtcct gctcatacat
    27241 accgtggaag cggatgagtt ggaagttgtc gcctttgaag ccgcccgtga gcgggtcttt
    27301 caaatcgtcc aaaatcgtgc cgcgtaaaaa attgctttcg gttttcagac gttcgttgtc
    27361 ggatagcggt ttttcttgcc acgccaaacc ttttgtcttg gtctgtacgg tcattttgtg
    27421 ttcctcccga ttatatttaa tcaataaaca tcacgctgat agcgtttttc ttcgcgcagc
    27481 atatccaaat attcttctgc gccctcttcg tccaaatgtc ctgccccgat aatcacatcc
    27541 agcaaggcgg cttccacgtc ttttgccatt tttgccgcat cgccgcacac atagatatgc
    27601 gcgccttcct gcagccattg ccaaagtcct tccgcctgtt cgcggatttt gtcctgcaca
    27661 tagatttttt cttcctgatc gcgggaccag gcgaaatcgt acctgtgcag gaaaccgtct
    27721 ttggcaaact gctgccattc ggtttgatag agaaaatcac gggcaaaatg cggattgccg
    27781 aaaatcagcc agtttttgcc ttccgcattt tctgcggcac gttgttggac gaaggcgcgg
    27841 aacggtgcga cgccggtgcc cgagccgatc atcacaatcg gcttgcggct gtcttcgggc
    27901 agcctgaagc cgtcgttgcg ttccacaaac acgcgcaccg tgccgtcctc ttccagccgg
    27961 tcggcaagga aacccgatgc gccgcccgcc ctggcgcgcc cttcgtgttc aaaacgcacc
    28021 gcgccgacgg tcaggtgcac ttcgtccccc acttccgcct gcgacgagga aatcgaatac
    28081 aggcgcggcg caagtgggcg cagcaggccg gcgaattgtt ccgccgtcag ttttgccggg
    28141 aagcggtgca gcacatcggc aatcggcgtg ctttgcacaa agccttgcaa aacggcgttg
    28201 tcggcagcaa tacggtcgag ttcgtcatca tcggcgaacg gggcatagcc tttgacaaag
    28261 gcgggggtgt tttgcgtgag ttcaaaatgg gataacagtg cggaggcaac cggcagggtt
    28321 tttccgcccg cctgtatttc cgttgccgga tcgatgccga gcaggtctag gatttccctg
    28381 accagtgccg gatcgttgtc aaaccaaacg ccgagcgcgt cgcccgggag gtagtgcaaa
    28441 tccgaaccgc tcaaatcgat ttcgatgtgg cgcacgtctt tatcggattg gcgggcggtg
    28501 attttctgat tggccagcag ggcggcggga aaggggtctg ccttgcagta cctgccctcc
    28561 gatgccgtct gaaggccggc ggggggcgtt gcctgcggcg cgggcgttgc ccggtttttt
    28621 gcggcttctt cttttaagag tgcggcgata ttgtctgtcc aggcgtttgc ggaggcggta
    28681 aagtccaaat ccgcatcaac gcgttcgagc aggcgtttcg cccccaattc ttcaaaacgt
    28741 ttgtcgaaat ctttgcccgc ccggcagaaa ttcggatagg aactgtcgcc caaacccaat
    28801 acggcaaatt ggagtttgtc caatttcggg gcttttttgc cgttcagcag tttgtgcagc
    28861 acgacggctt cttccggcgg ttcgccttcg ccttgggtgg acgtaaccag cagcaggcgg
    28921 cgttcgccgg cgatgttttt cgccttatag tctttcagtt cggcgcgact gacttggatg
    28981 ccggcggctt ccaggctgtc cgccgctttg tcggcaacgg atttcgcatt gccggtttgc
    29041 gaggcggaaa ggacggttac ggaaaaaggt tctgccgtcg gcaatgccgt ctgaagcgcg
    29101 ggcagtcctg cagatgcccc gtttcctgct tttgcccaag cgtagccgga cagccacgcc
    29161 cattgtgccg cgtccagccc cgacaggagc tgcgtgattt cgggcggcag aggcggtaat
    29221 ggcggatttg tgttctgcat atcgtgttca ctcataaaat catacctgcc gcaacagtgc
    29281 cgtatgtcgc ttcgtctatc aggataaacg aaccggcggc ggtgttttcc gcataaggcg
    29341 ttgccgtaac gggtttttga aggttgatgc ggactttggc gatgtcgttc atcttcaagg
    29401 attccgcgcc ggcctcttgt tccagcgtgc ggacatccaa aacgctttca atttccccga
    29461 cttttgccga cacggtttgc gtgccgtgct tgagcaggta tttgcgcgcg gtgttgagcg
    29521 gacgttcgtc aaaccagcaa agcgtgactt ccagatgttt ttgcggggcg agcggggaat
    29581 ttttatcgac aaaaaggtcg ccgcgcgaaa catcgatgtc gcgatccagc cggagggttg
    29641 ccgcctcgcc ggcaaaagcc tgcgccactt cgcctttggg tgtgatgatt tcggacactt
    29701 cggcggtcag cccgttcggt tcgatgcgga cggtttgccc gacggcgacc gaaccgcgtt
    29761 cgatgcgccc ctgatagcct cggaaatcat cggccttgtc ggcatcttgg cggacgacca
    29821 gttgcacggg gaaataaaaa tcgtcggcgg tgcggctgac ttcgtccgcc cccggcaggg
    29881 tttccaaaat ggacaataag ggttcgcctt tataccaagg catattgccg ccggggtaaa
    29941 caatgttgtc gcccaagagt gcggacaccg gtacgaaatg cgcgtctttc aaaccgagct
    30001 gttcggcaag tcggcggtat gcctccacaa tggcgttgaa tttgtcttcg ctgtaatcca
    30061 gcaggtccat tttgttgacc gccaccacaa tatgcgggca gttgagttgg cggaggatgg
    30121 cggaatggcg tttggtctgc ggcagaagct gcaagggctg cgcgccgaaa tccagttggg
    30181 atgcgtcaac cagcacgact gccgccgaag cggtgcttgc gcccgtaacc atattgcgcg
    30241 tgtattgttc gtgccccggc gtgtcggcga tgatgaattt ccgtttcgcc gtggaaaaat
    30301 agcggtatgc cacatcgatc gtaatgccct gttcgcgttc ggcttccagt ccgtcggtca
    30361 ggatggagaa gtctatggtt tctttcaaac ctttgctttt gccggattcc aaggttttga
    30421 tttggtcgga cagcagggct ttgctgtcgt agagcagtcg tccgattagg gtgcttttgc
    30481 cgtcatcgac gctgccggcg gtaatgaagc ggagcggggt ttggtgttgt gccgtcatat
    30541 tttcttcctc atatctgctt aaagggtttt tgaaatttag aaatagcctt cttttttgcg
    30601 tttttccatt gccgcctcgc ttgcctgatc gtccagccgg gtcgcgctgc gttcggaaat
    30661 gtcggcaacc gctgtttctc tgataatctc cgtcggcgtg gacgcggtgc tttctaccgg
    30721 gcaggtgcag ctgatgtcgc cgacggtgcg gaagcggaca tcaaggattt cggaggtttc
    30781 agacggcatt ttcggggtga gcggcgttac agggaccagc agccccctgc gtctgaccac
    30841 ttcgcgcctg tggctgtaat aaatcggcgg cagctcgagg ttttcgcggg cgatgtattg
    30901 ccagatgtcg agttccgtcc agttggaaat cgggaagacg cgcatatttt cgcctttgtg
    30961 cagcctggtg ttgtacagcg accacagctc ggggcgttgc gccttcggat cccattgtcc
    31021 gaactcgtct cggaacgaga aaatccgttc tttggcgcgg gctttttctt cgtcgcgccg
    31081 cgcgccgccc atcagcgcgt caaagccgtt tgcctcgatg gtttccaaca gcgtaaccgc
    31141 ctgcgccgca ttgcgcgaat cggtttcttt gcgtaaaact actgtgcctt tggcaatgga
    31201 gtcttccacg cgccccacta tcaggcgggc attgagtttt gccgcctgcg cgtcgcggaa
    31261 agcaatcact tcggggtagt tgtgccccgt gtcgatatgc accagcggga agggcagttt
    31321 taccggcctg ctgcccagcc ggaaggcttt gcaggcgagg gcgagcagga ccacggaatc
    31381 tttgccgccg gaaaagagca gggcggggtt ttcgcattcc gccgccactt cgcggatgat
    31441 gtggatggat tcggattcca accagtcgag ttgggcgttg ttcggttcgg ttttcgtcat
    31501 accatattcc ttatttcttc tgtctgatat ttatgaatta tttgtgcagc ccgcattctt
    31561 tgctgtttct gccttcccac caccaccgcc cggcgcggat gtcttcgccc gccttgacgg
    31621 ggcgggtgca ggggtcgcag cctatgctgg gaaatccttg ccggtacaaa tcgttgtaag
    31681 gcacattgtt ggcgaggatg tatgcccaca cgtcgtgttc cgaccagtcg aaaatcgggt
    31741 tgtatttgcc gatgccccgt ccggcatcgt attcggcaaa cggcagttcc gtgcgtgtgg
    31801 cggattgttc gcggcgttgc ccggtaagcc aggcgtccgc gcctgcaatg gcgcggttga
    31861 gcggttcgat ttttcggatg cggcagcatt cgcggcgcgc ttcaacgctg tcgtaaaagg
    31921 caaacctgcc tttgttttcc acataacggt cggcatcttc tcgaaccggc cggaaacgct
    31981 ttatccgcaa atggggatat gcgcgtccga gcctgtccag caggtttagg gtttccgtgt
    32041 ggagcagccc cgtatccaag gtaaaaatgc cgatattgag gttttcgccg gcgataaggt
    32101 cggtaatcac catatcttcc gcagcaaggc tgctggcaaa ccgtgcatcc cggtggctgc
    32161 cggcaatccg gtgcaggcgt tgtttgaggg tttccgtttt ttccgccaaa gctgtttcac
    32221 tgccattttc aatcggcgga attttccata aagcaggttt gaaaattttc attttgtccg
    32281 ccttgcttta tgcggcttgt tgttcttgtc cggtgcccag tccgtccaat gcaaaatacg
    32341 cgtgttcggc cggattgctt tcttttttgg cgttttcgcc gaaccaagcc agtttttcgt
    32401 gcagttccac cacttcgcct acaacaatta aagccggctt cggcgcgttt tcggcgaggc
    32461 gggcgaggtt ggcgagcgtg ccgattgccg ttgattgcgt tgattgcgtg ccgtggctga
    32521 tgacggcggc aggcgtgtcg ggcgggcggc cgtattgttg cagccgttcg gcaatcagcg
    32581 cggctttgag cgtgcccata taaatcacca aggtttggcg gctgcgggca agcgtctgcc
    32641 attcgatatc gggcgcatcc gccttgcggt ggccggtaac gaaaaccgca ctttgggcat
    32701 aatcgcggtg cgtgagcggg atgccggcat aggcggtcgc gccgacggcg gcggtaatgc
    32761 cggggacgac cgaaaacgga atgtggtggc gcgccagcac ttccaattct tcgccgccgc
    32821 gtccgaacac gaaagggtct ccgcctttca aacgcaccac gcgcctgcct tcgcgggcca
    32881 gcctgaccat aagcgcattg gtgtcctctt gcggggtgcg gtcgccccgg gcgcgcttgc
    32941 cgacaaaaat ccgttccgca tcgcggcgga cgagggacag tatgccgtct gaaaccagcg
    33001 cgtcgtaaag caccacgtct gcctgctgga tttcctgcag ccctttgagc gtcagcagcc
    33061 ccgcatcgcc gggacccgcg ccgaccagcg agacggagcc gccttgatca ttttgacgac
    33121 tttgttccaa ttggcctgcc aattcccgtt cggcaagggt gttttgccgg tttttgacga
    33181 gggctgcgaa acgtccgtta aactgctttt cccaaaagcg gcggcgttcg gtaacggatt
    33241 tcagtttgcc cttgaccgca tcgcgccatc ttccggagat ttccgccata tcgcccaaag
    33301 acggcggcag cagggcttcc agcctttcac gcagcaggcg ggcgaggacg ggcgcgctgc
    33361 cggagctgga aacggcaatc tgaaccgggt tgcggtcgat aaccgacggg aagatgaagc
    33421 tgcaatggtc gcggtcgtcc accacgttga ccggcttttg gcagctttcg gcaagatgga
    33481 aaacgcgccg gttgagggct tggtcgctgc ttgccgcaat gatgaggaaa actgtgcgga
    33541 tgtgttcggc acgaaattct tcggcaagcc acaggatttt gttttccgcc gccaacgcgg
    33601 agagttcggc attcaggtgt tttgcggcaa ccctgacctc tgcgcccgcc ttcagcagca
    33661 ggctgatttt gcgtgcggcg accgcgccgc cgcctacgac caatacgggg cggccggcga
    33721 ggttggcgaa aatagggaaa taattcactg gctgactcct ttgctgtttg cccgcacctt
    33781 gtttccgata cggtgcgtcg cggcattttt gtcggaatgc gggtcatttt agacaaaagg
    33841 attttcccgg ttaaataata aaaaggcatt tgttagaagc tgaaagctat atgagggcgg
    33901 ctgcggatac ggcggttttc cgttttatag tggattaaca aaaaccagta cggcgttgcc
    33961 tcgccttagc tcaaagagaa cgattctcta aggtgctgaa gcactaagta aatcggttct
    34021 gtactatccg tactgtctgc ggcttcgtct ccttgtcctg atttttgtta atccactata
    34081 aaacggtttg ggacggaaaa acggcctgaa gccgtttcgg gcattcagac cgtttgcgcg
    34141 gagaggggat gccgtccgaa gggcgaaaag ggcttcagac ggcattgatg tcgggtttca
    34201 ggacaggagc aggatggcgg ctgcggcaag cgcggcaacc gataatgcgg cggcaagcgc
    34261 ggttttgccg gcaaagcgga ttgaggtttt gccttcgatg tatttgaagc cggttatcat
    34321 cgggaggacg aggtttttct ttttgaacac gcggtatgcg gcgacggcgg cgatatgaat
    34381 ggcggaaaaa acggcgagca gcttgaaaaa gttgaggtgg attttccgca taaggctgcc
    34441 cgtatgctcg gaaaccaaat ggttgaggta gccgttggtg ctgaaggtgt tttcatcggc
    34501 ggcaaaaagc cctgtgccga cttgaaatga gacggcggcc aaaagcgcga cgaccatcag
    34561 tgcgcccaag gggttgtgtc cgggttggac gtgttcggga ataccgtttt tcatatactc
    34621 tctgataccc gaccatccgc ggacgaaacg ggagaaacgg gcggtatcgc tgccccaaat
    34681 gccccagcag aggcggaata cgagcaggaa aaggataaac agcccgacgc gcgtgtgcca
    34741 ttgcagcata tcgccgccgg ttttcgcgct ataccacata aagggtaggg atgcggcaag
    34801 cagccagtgg gaaaggcggg tggggaagtc ccagactttg cttttgtttt tcataatcgg
    34861 tttccggcgg tagaatcggt ttgttttcga gccttaattt taaccgattg gaggggaaat
    34921 gtttcccgtt ttccatcttt caggcgaaag ccgccgccgg atgcttcagg cggcatcgcg
    34981 ttttccctat gttttcaaag cccgtgcgga agattcgcac aaagggactt tcggcacgct
    35041 cgccgtagtc ggcggcgcgg tcggcatgag cggcgcgccc gtattggcgg catcggcggc
    35101 aatgtatctc ggctgcggca aagtgtgggc gggtttcaat caggaaacgc tgcctttcgc
    35161 cgtcattgcc ggttttcccg aaattatgtt ggacacggca gacggtttga ccaagcgtca
    35221 agatataaac gcctgggttg tcggttgcgg cttgggtaca ggtagggcgg cggtcagaac
    35281 gcttgccgga attttggctg aacacacgga caagcccgtt cttttggatg cggacgcgct
    35341 gaacatatta tcaaccgatg ccgaaacccg aaatctggca cgcgagtgta aaaacctgat
    35401 tttaacgcca gaccccgccg aagccgcgcg cctgcttgga acgacggttg cacaggttca
    35461 ggcggatcgg acggcggcag tgaggaagat aggggcaatt ttcggcgcaa ccgtggtttt
    35521 aaaggggcac aaaacattgg ttgccgcatc cgatacggaa atctatgtca acgaaagcgg
    35581 caacgcggga ttggcaacgg cgggcagtgg cgacgtattg ggcggcatca tcggcagtct
    35641 gctcgcacag ggcgtgccgg tttttgaagc cgcctgcgcg ggcgcgtggc tgcacggcgc
    35701 ggcggcggat gtcataaaag aatcggcagg cattgcggca gggctgttgg caggggaaat
    35761 cgccccggcg gcaaggtggc tgcgtaaccg gataactgaa agtatgtaag aagatatagt
    35821 ggattaacaa aaaccagtac ggcgttgcct cgccttagct caaagagaac gattctctaa
    35881 ggtgctgaag cactaagtga atcggttccg tactatccgt actgtctgcg gcttcgccgc
    35941 cttgtcctga tttttgttaa tccactatac cgtctgaaag gcaagggctt cagacggcat
    36001 cttcatttcc caaatactgt ccggtaaagc gtggtataat gcgcagttat ctcttacagt
    36061 ttttgaaaaa cattaattat gaaacaaatc cgcaacatcg ccatcatcgc ccacgtcgac
    36121 cacggcaaaa ccacattggt cgaccaactg ctgcgccaat ccggcacatt ccgcgccaac
    36181 cagcaggttg acgagcgcgt gatggacagc aacgacctcg aaaaagaacg cggcatcacc
    36241 atcctcgcca aaaacaccgc catcgattac gaaggctacc acatcaatat cgtcgacacg
    36301 ccgggacacg ccgacttcgg cggcgaagtg gagcgcgttt tggggatggt ggactgcgtc
    36361 gtcttgttgg tggacgcgca ggaaggcccg atgccgcaaa ctcgtttcgt gaccaaaaaa
    36421 gccttggctt tggggctgaa accgattgtc gtcatcaata aaatcgacaa accgtccgcc
    36481 cgtccgagct gggtcatcga ccaaactttc gagctgttcg acaacttggg cgcgaccgac
    36541 gagcagttgg atttcccgat tgtttatgct tccggtctgt ccggtttcgc caaattggaa
    36601 gaaaccgacg agagcaacga catgcgtccg ctgttcgata ctatcttaaa atatacgcct
    36661 gcaccgagcg gcagcgcgga cgaaacgctg caactgcaaa tttcccaact cgactacgac
    36721 aactacaccg gccgcctcgg tatcggtcgt atcttgaacg gacgtatcaa gcccggtcaa
    36781 gttgttgccg tcatgaacca cgatcaacaa atcgcccaag gccgcatcaa ccagcttttg
    36841 ggtttcaaag gtttagaacg cgtgccgctt gaagaagccg aagccggcga catcgtgatt
    36901 atttccggta ttgaagacat cggcatcggc gtaaccatca ccgacaaaga caaccccaaa
    36961 ggcctgccga tgttgagcgt ggacgaaccg acgctgacca tggactttat ggttaacacc
    37021 agcccgttgg cgggtacgga aggcaaattc gtaaccagcc gccaaatccg cgaccgcctg
    37081 caaaaagaat tgctgaccaa cgtcgccctg cgcgtggaag acaccgccga tgccgacgtg
    37141 ttccgcgtat ccggacgcgg cgagctgcac ctgaccattt tgttggaaaa tatgcgccgc
    37201 gaaggctacg aactcgccgt cggcaaaccg cgcgtcgtgt accgcgacat cgacggtcaa
    37261 aaatgcgaac cttatgaaaa cctgactgtg gacgtacccg acgacaacca aggcgcggta
    37321 atggaagaac tcggccgccg ccgtggcgaa ctgactaata tggaaagcga tggcaacgga
    37381 cgcacccgcc tcgaatacca tattccagcg cgcggcttga tcggtttcca aggcgaattc
    37441 atgaccctga cgcgcggtgt cgggctgatg agccacgtgt tcgacgatta cgcgcccgtc
    37501 aaacccgata tgcccggccg ccacaacggc gtgctggtgt cccaagagca gggcgaggcg
    37561 gtcgcttacg ccttgtggaa tcttgaagac cgcggccgta tgttcgtatc gcccaacgac
    37621 aaaatctacg aaggtatgat tatcggcatc cacagtcgcg acaacgattt ggtggtcaat
    37681 ccgctcaaag gcaaaaaact caccaacatc cgcgccagcg gtaccgacga agcggtgcgc
    37741 ctgactacgc cgatcaaact gactctggaa ggcgcggtcg agtttatcga cgatgacgag
    37801 ctcgttgaaa tcacgccgca atccatccgt ctgcgcaagc gttacttgag cgaattggaa
    37861 cgccgccgcc actttaaaaa gctggattaa tgtttaaatg atacgcgatg ccgtctgaaa
    37921 aatttcagac ggcatttttt attcggacgg gctttgcggc ttcttgaagc tgtttcagac
    37981 ggcgtttttc ctacccaatc aagaaactgc cgccattttt ccagcggcat atcgccccgt
    38041 cgcgtttcgg tatcgggttc ggcttcccgg cagcatgtcg aacaatgcct tttgaaggaa
    38101 tacgcctttt gagtccttac aagcttagga agaggatata gtggattaac aaaaatcagg
    38161 acaaggcgac gaagccgcag atagtacaga tagtacggaa ccgattcact tggtgcttca
    38221 gcaccttaga gaatcgttct ctttgagcta aggcgaggca acgccgtact ggtttttgtt
    38281 aatccactat aaaatatacc gcaaaacata ccataaaaga aacggtaacg gatagttgta
    38341 agcggttgat gacgattata gacaataacg ggttttccca atgaaattat tgtttgaata
    38401 ataaaaaatc ccaaaccgta aaagtttggg atttgtattt ggcagagagg aagggattcg
    38461 aaccctcgat acgctattca cgtatacacg ctttccaggc gtgcgactta aaccactcat
    38521 ccacctctct aatggcggaa attatcccaa tcgggataat ttattatttg gtgcccagga
    38581 gaagactcga acttccacac ccgtgaggat accagcacct gaagctggcg cgtctaccaa
    38641 ttccgccacc cgggcaatct aaatattgaa ataaagcaaa gcatttgatt tggtgcccgg
    38701 gagaagactc gaacttccac acccgtgagg atactagcac ctgaagctag cgcgtctacc
    38761 aattccgcca cccgggcttt gctttatttg ctgttttgcg gtacagtgtc ctgccgcaaa
    38821 gaagtcgcta ttatatagtt cataaagaga atgtcaacag tccaaatgaa taaaaatatt
    38881 aaatctttaa atttacggga aaaagacccg tttttaagtc gtgaaaaaca gcgttatgaa
    38941 catcctttgc ccagtcggga atggataatc gaattgttgg agcgcaaagg tgtgccttca
    39001 aaaatcgaat cgcttgcgcg cgagctgtcg attacggaag acgagtatgt cttttttgaa
    39061 cgccgtctga aggcgatggc gcgggacggt caggttttaa tcaaccgtcg gggcgcggtt
    39121 tgcgcggcgg acaaatcgga tttggtcaaa tgccgcatcg aggcgcataa ggacggtttc
    39181 ggttttgccg tgccgctcac gcccgccaaa gacggtgatt ttgttttgta tgaacgccag
    39241 atgcgcggca ttatgcacgg cgatattgtc actgttcgtc ctgccggcat ggaccgtagg
    39301 ggccgccgcg aagggacggt tctggatatt gtcgaacgcg cgcaaagcaa agttgtcggc
    39361 cgtttctata tggatagggg cgtggcgatt ttggagccgg aagacaagcg tctgaaccaa
    39421 agcatcgtat tggaaccgga cggcgtggcg cgtttcaaac ctgaatccgg tcaggtcatc
    39481 gtcggcgaaa ttgaggttta tcctgagcaa aaccggccgg cagtggcaaa aatcatcgaa
    39541 gttttgggcg attatgccga cagcggcatg gagattgaaa ttgccgtgcg caagcatcat
    39601 ttgccgcacc aattcagtga agcgtgtgcc aaagccgcga aaaaaattcc cgaccatgta
    39661 cgcaaaagcg atttgaaagg ccgcgtcgat ttgcgcgacc tgcctttggt aacgatagac
    39721 ggcgaaacgg ctcgagattt tgacgatgcg gtgtttgccg agaaaatcgg acgcaattac
    39781 cgtctggtcg tggcgattgc cgatgtcagc cattatgtcc gccccgatga cgctatcgac
    39841 acggacgctc aggaacgcag caccagtgtt tacttcccgc gccgcgtgat tcccatgttg
    39901 ccggaaaacc tgtccaacgg catctgctcg ctcaatcctc atgtcgagcg tttgtgtgtg
    39961 gtgtgcgata tggttatcac ttacgcgggc aatatcaaag aataccgctt ctaccccgcc
    40021 gtgatgcgct ctcatgcccg cctgacctac aaccaagttt ggaaatggct ttcaggcggc
    40081 atcgagcatc cgttcaaaac ccaaatcgac acgctttaca aactcttcaa aatccttcag
    40141 aaaaagcgtt tcgaacgcgg ggcggtggag tttgacagca tcgaaaccca aatgcttttc
    40201 gacgacaacg gtaaaattga aaaaatcgtc cccgttgtcc gcaacgatgc ccacaagctg
    40261 attgaagaat gtatgttggc ggcaaacgtt tgcgcagcgg attttctgtt gaaaaacaag
    40321 cataccgcat tgttccgcaa ccatttgggg cccacgcccg aaaaactcgc cgccttgcgc
    40381 gagcagctcg gtctgttggg gcttcaactt ggcggcggcg acaacccgtc gccgaaagac
    40441 tatgccgcgc ttgccggaca gttcaaaggc aggccggatg ccgaattgct gcaagtcatg
    40501 atgttgcgct ccatgcaaca ggcggtttac gaaccgcatt gcgacggaca ctttggtctt
    40561 gcctacgaag catacgccca cttcacctca cccatccgcc gctatcccga cctgaccgta
    40621 caccgcgcca tcaaagccgt gttgaatcag caaacctaca cgccaaaaaa aagctggcag
    40681 gctttgggcg tgcatacctc gttctgtgag cgccgtgccg acgacgccag ccgcgacgtg
    40741 gaaaactggc tgaaaaccta ttatatgcgc gataaggtcg gcgaagtatt cgaaggtaaa
    40801 atctccggca tgaccagttt tggtatcttt gtaacactgg acggcatcca cattgacggc
    40861 ttggtgcata tcagcgattt gggcgaagac tatttcaact tccgccccga aatcatggca
    40921 atcgaaggcg aacgcagcgg catccgtttc aacatggggg acagggttgc cgtccgggtc
    40981 gcccgtgccg atttggatga tggaaaaatc gattttgtcc tgattgccga ggggagcggc
    41041 agggggcgga aagctaaatc atccgcgtct gtcaaaccgg cagggacggc ggggaaaggg
    41101 aaaccgaaaa ccgccgccga gaaaaaaaca gcccgaggcg gcaaagtaag gggaaggggc
    41161 gcgtctgccg ccgcagaatc ggggaaaaag gcaaagaaac cggttccgat taaggtaaaa
    41221 aaacggaaag gcaaatcata atgctgacgg ggtggcttgg ggagggcggg gcatactttc
    41281 ccgaagccgc cgcccgattt tttgatatgg cggccggcgc atcatttcag gccaatcgaa
    41341 acgcccgtat tgaaagattg cgtttatttc caccgccgtt ttgaaggccg gcggtattcg
    41401 gcagacgggg cgcaaacggc gttcagacgg cattttcatt ctttcggcgt gtccgtccga
    41461 attgctttgc ccgtccgcgc aatcagcctg tgcggcttgc cttgaacaga caaaaaatgc
    41521 cgtctgaaac ccggaaatca ggtttcagac ggcattttgc cttgaaaagg ctgttcaaat
    41581 cagcgatggt agttcggtgc ttctttggta atttgaacgt cgtgaacgtg cgattcgctc
    41641 atacctgcgg aagtgatttc cacaaattcg gctttttcgt gcatttcggc aatattggcg
    41701 caacccaaat accccatgct ggagcgcagt ccgccggtca gttggtggat gatgttcaca
    41761 atcgggcctt tgtaaggcac gcggccttcg ataccttcag ggacgtattt gtcggtgctg
    41821 tcggttttgt cttggaagta gcggtcggca gaaccttggc tcatcgcgcc caaggaaccc
    41881 ataccgcgat aggatttgta cgagcggcct tggtagagtt cgatttcgcc cggtgcttct
    41941 tccgtgccgg caaacatacc gccgagcatg acgctgtacg cgcctgcggc gagggctttg
    42001 gcgatgtcgc cggagaagcg gatgccgccg tcggcaatca gcggaacgcc cgtgcctttg
    42061 agggcttcgg caacgttgtg aatggcggtc agttgcggca cgccgacacc tgccacgata
    42121 cgggtggtgc aaatcgaacc cggaccgata ccgactttga cggcatccgc gccggcggcg
    42181 accaaatcca atgcggcttt ggcagtggcg atgttgccgc cgatgacttg gatgtgcgga
    42241 taggtttctt tcacccattt cacgcggtca atcacgcctt ggctgtgccc gtgggcagta
    42301 tcgacgacaa tcacgtccac gccggcttcg accaaggctt tgacgcgctc ttcggtatcg
    42361 ccgccggtgc cgactgccgc accgacgcgc agacggcctt cggagtcttt gttggcgttg
    42421 ggaaactcgg tggtttttaa aatatcttta acggtaatca gacctttgag ttcgtctttt
    42481 tcgttcagaa ccaaaacgcg ctcgactttg tgcgtgtgca tcagttcgcg cgcttcgtct
    42541 atgcttgtgc cttcggggac ggtaaccaaa cgttcgcgcg gggtcataat ggcggaaacg
    42601 ggcaaatcga cgcggttttc aaaacgcagg tcgcggttgg ttacgatgcc gacgactttg
    42661 ccgttttcaa cgacgggcag gccggacatt ttgcgtttgc gctgcgcacg catttccaag
    42721 acttcgcgga tgagcgtcgt cggtgcaacg gttacggggt ctttgaccac gccgctttcg
    42781 tggcgtttca ctttggaaac ggcgcgcgcc tgcatttcgg gcggcatgtt tttatggatg
    42841 atgccgatgc cgccttcctg tgccatcgaa atggcgaggc gcgcctcggt aacagtgtcc
    42901 atcgcagcgg aaagcagggg gaggttgagt gtgatttcgc gggtgagctt ggtttgaagt
    42961 ttaacgtctc gcggcagcac ggtcgaatgt gcgggaacca acaaaacatc gtcgaaagta
    43021 taggcttttt ctacgatacg cataatgctc ggtctttcag tttgtgcaag atgcacggca
    43081 ttatagcacg ttaccggcgg cttgacagtt tatcaggttt aattttggtc ccctttgaat
    43141 agcccggttt tcctttgccg accactgttg ctcccgttct ttcaatttca ggaaaagctt
    43201 ttttctaatt tttggtaagt ggctcagtta ttgaagccct atatcgggcg gtaacttcca
    43261 gcacaaaaaa acggagtagt tctttattta ttttttcctt taattttcag tatattatct
    43321 taatatttcg agggtaacat atctgctaat ctagttacag ccccatatat tatagattca
    43381 attgaaaaat aacagattca actgtacctt tcttatacct gatttcttta aagtttttcc
    43441 cattgtcaaa actataaaaa agtctctctt cctcaccaga atatcccatt tctaatacta
    43501 aacaatctat ttcacaatta ccccctccac tcatatatgt agtatcaata gaaaatatat
    43561 tgcataattc cagagaatta tatttatcta tatcaaatag ttgctctcca aaatctaaac
    43621 cattgtttcc acctgcataa tacttctctt caaaggatga cttcaaacta ttaaaaactg
    43681 catttagcgt aaaatatcca tatgggcata tcttaggata tatattctct cctattatta
    43741 tctgaatttt tccataaata aaaccattac attcagagtt ttcttcccat aaaatcccaa
    43801 attttcttgt tgagtccata ataatattca tataaatcct tatattaata aattatttac
    43861 aatatccccc gctttcagac ggcatacggc gtggcggcgg aatgccgtct gaaggcgggc
    43921 gttatgataa ttgttccagc agcgtctgct tcaatgagga ctggattttt gggtttttca
    43981 ggtctaggct aaaaacggta aaactgtctt cggcgcgttc atccagtgtg gagattttgg
    44041 catagcgcag gctgacgttg tgggcgaaaa agacttccgc catatcggcg agcaggaagg
    44101 ggcggttgac ggcggtgatt tcgacggagt accaatcggg gtagtcttct tcgggggtga
    44161 tggtgatgct tggcgcgatc ggcatatagc ggctgcggcg gctgatgcgg cggctatggc
    44221 tggagatttc ggcaacggtg tgtccgtgga taaagctgtt gagttcggct tcgagcgcgc
    44281 tttggatgtc ggggtagtct tcgggagcgt gctgcgaggg gatttgcacg atgaaggtgt
    44341 cgaggatgta gtcgtgttcg gtgataaagg cgcgggcggc gaggatatcg aagccgtggc
    44401 ggctgaaaat gcggcagagg cgggcgaaca ggcgcgggcc gttgggcatg aaaaccatga
    44461 cttgaaagct gttgcttttg ggcaggatgc ggctgcggac gatgggggtt tcaaaatcgt
    44521 gtacgaggtt ggcggcgtgc cacaggattt cgcgggactg gtggcgggcg aagtaggcgg
    44581 aaccgagcgc gttccatagt tttttctgct gtttttcggg gacggcggcg cgggtgagta
    44641 agtcggcggc ttcctgccgg cggcggccga agagggtgtg cgggttgccg ccgttgcctg
    44701 taaggtagcg tccggcggca tggaagaggc tttccagcag gctggcgcgc catgcgttcc
    44761 acagcttggg attggtgccg cgtatgtcgg aaatggtcag aaggtagagc gcgctgaggc
    44821 gttcgtgggt ttggacgcgt ttgcagaagg catcgagtac gctggggtct tggatgtctt
    44881 ctttttgggc gacggtggac ataagaagat ggttttcaac cagccaggcg agcaggtcgc
    44941 tttcttcttc ggtcaggaag tggtcggcgg caaattggcg cgcgtctgcg atgccttgta
    45001 tggcatggtc gccgccgcgt cctttggcga tgtcatggaa aaaggcggca aggtagagga
    45061 tgtcttgttt ttcaaaggac tgcatcagtg cagaggcgta gggcagctcg tggctgtgca
    45121 tatccaaggc aaggcggcgg acgttgcgga cgacggtgag gatgtggtcg tccacgggat
    45181 agatgtggaa caggtcgtgc tggagcaggc cgacgatttt ttcccacgcg ggcaggtagc
    45241 gtcccaatac gccgtagagg ttgaggaagc gcagggtttg ggtcagtccg ttgccgttgc
    45301 ggaaaaagcc ggcgaagcgg tggcggtttt cagaattttg gtagaagctg cggttgattt
    45361 tgcgcgtcgc gccccaccat gcgcgcaggg tttgcggttc gagcgcggta atatcgttgc
    45421 gctgctgcat gatttcgacg attttgaaaa tgtgttcggg ccgtctgaaa aaaatatcgg
    45481 tgtgccgcgc ggcaatttgg ttgttgactt ggatgtagtc gtcgtcaatc cgcagggtaa
    45541 cgcgcaacgg ggtggaggaa acgcggcttt gcagcatagg cgtgaggatg ccgcccagtt
    45601 gtttgacggt tttaatcgcg cggtaaaaca cgcgcatcag ttcttcgctt tggcggcgga
    45661 ggttcaagcc ttcataaccc atgctttcgg cgacttgcgg ctgcaaatcg aacagcaggc
    45721 ggtcttcggc gcgcttggcg tttaaatgca gatggatgcg gatgtgggcg aggcggcggt
    45781 agccgtgcga aagcataccg gcttcggcac gcgtcaaaat ccgctgtttg agcaggtcgg
    45841 gcaggtcggt cgccaagcct tgcgccttcg ctatccaaag cagggtgtgg atgtcgcgca
    45901 gaccgcccgg acagcttttg atattcggct ccaatactgc ccccgaacct tgcgatttgg
    45961 cgtggcggtg ttccatctcc accagttttg cctcgacaaa cgccgccaca ttgcgctgcg
    46021 cgttcatttt tttcgccagt tcgtccgccg tttggcggtt gccgaacaaa aaacgggcct
    46081 ctaaaaacgc cgtgtccccc gtaatatcgt cgcgcacgct ttcgcacagt tcgccaatgc
    46141 cgccgctttt tacagacggc atcagtttgc agtcccatag ggtttgaaca aaccgggcaa
    46201 tctgttcctg aatgccgtct gaaagcgggg caggggagac aaccgccaaa tccacatccg
    46261 aacagggata cagttcgccg cgcccgaagc cgcccaccgc catcaggcat aacgcgctgt
    46321 tttgaaaata ctctgcccac aatgccgcca gtaaggtttc gactgccgcc gtgtattctc
    46381 tgaaaaatac cgacacgcgg ttggctttca aataatgcgc ttcggcggca tcgcgctgct
    46441 gtttgaaggt ttccagtgct gaagacaggt ttgcaggcat ttttattctt tcgattggcg
    46501 ggaaaaaagg agacggatgg ttcggcggtc aaataccgct ttcagacggc atttgtcggg
    46561 tatcggcgga attggtcatc aggttcaacc gttcctgcgg cggcagcagc aactgatacg
    46621 ccgccacgcc caaagccgcc gccatttttt cgatattcga cagggcgatg ttccagcgtt
    46681 tgcgttcgac tgccgacaca taagtcctgt ccaaaccgca ttgccgcgcc aattcttctt
    46741 gcgaccaacc cttgttcacg cggaaaagcc gcatattgta tgccaatacc gcccgcaaat
    46801 cctgttcgtc aggcagttcg gcaggcagag tcaatttgtt gcccatcatt tgcttccaaa
    46861 taaatggtta aagttaagca tttgctgtta cggattttac ttcacataaa agccaaaaaa
    46921 aaggggtggc ggcagccaca cccaaacaca cacacatcaa gaggaaagag gtaacatcaa
    46981 gacagattgg gaaacagcaa acaagcaatc tagatttcat atcggtatta tcaaacaaaa
    47041 accgttatcg agtattgatt caaatcaatt tagatttgat tcgcaaaccg gcaaagaaaa
    47101 acggcatacc cgtttatacg gatatgccgt ctgatgatgc aggggactgc tgtatttggc
    47161 agtggtttgt gttttagtcc tcttgcgccg ctgtttgcag gtagttcggc aaaccaattt
    47221 tgccgattag ttcctgctgg gtttcgagcc agtcgatgtg ttcttcgttg gtgtcttttt
    47281 gtttttccaa caaatcacgg ctgacataat cctgttgcgc ttctgccacg gaaattgccg
    47341 caatcaatgc ctcatgtttt tctttctctt tttccaaatc gcaggcgatg atttcttcgg
    47401 tggactcgcc aatcagaagc ttgcccagtt cttgcaggtt cggcagacct tcgaggaaca
    47461 gaatgcgctc gatcaaatcg tcggcagctt tcatttcaac gatggactgt ttgaagaaat
    47521 gttcgcccag ttcttcaaag ccccagtttt tcaaaatacg ggcgtgaagg aaatattggt
    47581 tgatggttac cagcagcaag cctaagtttt tgtttagctc gcggataacc agacggtcgc
    47641 ctttcatacg gactcctttt attccgaata cggattacag ttggctttgg tagtagttgc
    47701 cttcgccaat taactcgatc aggcgcagct gctgttccag ccagtgggcg tggtcttctt
    47761 cggtatcttt cagttgggca accatcaggt cgcgcgtaac atagtcttga gcctcttcgc
    47821 acagtttgat gccttttttc aacgcatcac gtacttcgta ttcggtttgc aggtcggctt
    47881 tgaggcagga aaccacgtcc gtgccgatat tcagttcggc gcgcgccatt ttcggcgtgc
    47941 cgcccagcat caggatgcgg cggatgaagt cttcggcgtg tgtggtttct tcttccatct
    48001 cgtgattgag acgttcaaaa agtttggtgt agccccattc ggagtagagg cgggagtgga
    48061 taaagtattg gtcgcgtgcc gccagctcgc cagacagcaa ttcgttcata taatcaacaa
    48121 cagcttgatt gccttgcata atatctctct ttctgtaact tggttttcgg tatgtctgaa
    48181 cagcatctgt gtcgctattc gggatgcgtg cattgtatgc aaaagctgtc ggcatgacaa
    48241 attttctatt taaaatacaa acaattatca aaagaaatag ggcgtattcc ccgatgcctt
    48301 ccaaatcagt atgctgtttc ttatcggttt ttttgctgct aaaactaaga atccatctca
    48361 tccataaaga acatttacac ttattaacca tagcgaaaag caaatactgt acggtttttt
    48421 atcaaaattt ataatgaatc gttctcatta accgacagat tcttttgaga ttatcggatt
    48481 ttgggaataa attatgccgt ctgaggggct ttcagacggc atagacaatt aaagctgatt
    48541 aaacaacgcg gcagacagca gcgtaggaaa ttatttttta cttcggaacg gtattttggg
    48601 tttaatgatg cccgcaaccg ccgtggcttt cgcgtaaggc ttcggcagcc tgttcgtcgg
    48661 cgtggtagct ggaacgtacc atcgcgccga tggcggcatt gctgaagccc agttcgtatg
    48721 cttctttttc aaagattttg aactgctcgg gcgtaacgta gcgcaggacg ggcaggtgtc
    48781 cgtctgaagg ctggaggtac tgtccgatgg taatcatttc gatattgtgc gcccgcatat
    48841 cgcgcataat ttcacgcacg tcttcgtctg tttcgcccaa gccgaccatg atgccggatt
    48901 tggtcgggat gtgcggcatc atttctttat aacgttttaa taagtctaaa gaatgttgat
    48961 aattggcacc gggacgggct tttctgtaca ggctcggatg ggtttctagg ttgtggttca
    49021 tcacgtcggg cggggtttcg gcaaggattt tgagtgcgat gtccaagcgt cctcggaagt
    49081 cggggacgag gatttcgatt ttggtgttcg ggctggtttc gcggatggct ttgatgcagt
    49141 cggcgaaatg ctgtgcgccg ccgtcgcgca ggtcgtcgcg gtcgacggag gtgatgacga
    49201 cgtaacgcag gttcatggct ttgacggatt cggcgaggtt tctcggttcg tcggggtcga
    49261 gcatattggg ccgaccgtgt cccacgtcgc agaacgggca gcggcgggtg cagatgtcac
    49321 ccataatcat gaaggtcgcc gtgcctttgc tgaagcattc gccgatgttg gggcaggagg
    49381 cttcctcgca aacggtgtgc atcttttgtt cgcgcaaaat gtctttgatt tcaaagaatt
    49441 tgcgcgatgg gagtttggcg cgtatccatt cgggcttttt cagtttttcc tgaaggggga
    49501 cgactttgat ggggatgcgc gcggttttgt ccgcgcctct gagtttgatg ccgcgtttgg
    49561 ggtcgtcggt tttgatttca ctcattgttg tctgctttcg gtgtgaattg tgtttcaagg
    49621 tgtgcggtaa gtttggcggc gacttcgtcc ggcgtggggc agggttggac aaaatccgcg
    49681 atttgcgtca tttccatacc ggcgtagccg caggggttga tgtgggtaaa cgggcttaaa
    49741 tccatattga cgttgagcgc aagcccgtga tagacggagc cgtttttgat acgcagcccc
    49801 agtgaggcga ttttgcgttc gccgacgtaa acgccggggc gtttggggtc tgccgccgct
    49861 tcgatgccgt attccgccaa tgtggcgatg atgctgtttt caagcgcgga aacgatgttg
    49921 cggacgctgg ttttgcgccg tttgaaatca atcatcgtat aaacgaccaa ttgcccgggt
    49981 ccgtgatagg taatctgccc gccccggtcg atttggacga cggggatgtc gtccctaatc
    50041 agcaggtgct cgggttttcc cgccagtcct tgtgtaaaga cgggcgggtg ttcgacgacc
    50101 cacagttcgt cttcggtgtc agcattccgt ccggcattaa aggttttcat cgcttcaaaa
    50161 gtcggcagat attcgaccaa acctttgtgt atgattttca tctcaaagta ccactttgac
    50221 cagttcgtgc gaagtcagcg cgcggtagat gttgtccaat tgttcttggt tttcaacctt
    50281 tacctgtacg gtggcgccgg tatagttgcc tttgctgctc ggacgcgtgg tgatgtggtg
    50341 cgcctgcgtg tcgggggcgt ggaggcggac ggtgtccaat accgcctgct cgaactcggg
    50401 atgcaccgcg cccattactt tcaatgggaa ggtgcaggga aattcgatga gggatgtttt
    50461 gtttttttgt tcggtcatga tgtgctgcct tgtcgtgtac ggtatgccgt ctgaaggcgg
    50521 gtttgccttt cagacggcat cggatgtgcg ttattttagc ctaaaccgcg ataacaggct
    50581 atcgggaagg cggaggcttt tttgacggcg cggcggttct gctatactgg cgcgcaatat
    50641 tatttttaga agggtggttt ttatgtatcg gaggaaaggg cggggcatca agccgtggat
    50701 gggtgccggt gcggcgtttg ccgccttggt ttggctggtt ttcgcgctcg gcgatacttt
    50761 gactccgttt gcggttgcgg cggtgctggc gtatgtattg gaccctttgg tcgaatggtt
    50821 gcagaaaaag ggtttgaacc gtgcatccgc ttcgatgtct gtgatggtgt tttccttgat
    50881 tttgttgttg gcattattgt tgattatcgt ccctatgctg gtcgggcagt tcaacaattt
    50941 ggcatcgcgc ctgccccaat taatcggttt tatgcagaac acgctgctgc cgtggttgaa
    51001 aaatacaatc ggcggatatg tggaaatcga tcaggcatct attattgcgt ggcttcaggc
    51061 gcatacggga gagttgagca acgcgcttaa ggcgtggttt cccgttttga tgaggcaggg
    51121 cggcaatatt gtcagcagta tcggcaacct gctgctgctt cccttgctgc tttactattt
    51181 cctgctggat tggcagcggt ggtcgtgcgg cattgccaaa ctggttccga ggcgttttgc
    51241 cggtgcttat acgcgcatta caggtaattt gaacgaggta ttgggcgaat ttttgcgcgg
    51301 gcagcttctg gtgatgctga ttatgggttt ggtttacggc ttggggttgg tgctggtcgg
    51361 gctggattcg gggtttgcca tcggtatgct tgccggcatt ttggtttttg ttccctattt
    51421 gggcgcgttt acaggactgc tgctggcaac cgtcgccgcc ttgctccagt tcggttcgtg
    51481 gaacggcatc ctatcggttt gggcggtttt tgccgtagga cagtttctcg aaagtttttt
    51541 cattacgccg aaaatcgtgg gagaccgtat cgggctgtcg ccgttttggg ttatcttttc
    51601 gctgatggcg ttcgggcagc tgatgggctt tgtcggaatg ttggccggat tgcctttggc
    51661 cgccgtaacc ttggtcttgc ttcgcgaggg cgtgcagaaa tattttgccg gcagttttta
    51721 ccggggcagg taggcggttc cgaaacatat ttgaagcgga atacaacctt gcccgggttt
    51781 aaataaaaat gccgtctgaa ccccgaaaac agagcttcag acggcatttt catcacggct
    51841 tatttggcgg ttttgctgct gtcgataatt ttcataccgg cagaaatcag gctgccgatg
    51901 tcggcaacat tggcgggcat aatcagcgta ttgctttctt tggcaagatt gttgaacgcg
    51961 gcgacgtatt gttccgcaat cttcagattg accgcatccg caccgccttg ggtttgaagg
    52021 gcggcggcaa tttgacggat ggcttcggca ttggcttcgg caacaaggcg caaggattcc
    52081 gcttcacctt tggcgcggtt gatgcgggcg attttttcgg catttgacgc attgaccgca
    52141 gcctgagcct cgccttcgga ttgttggatt tcggcttcgc gctgaccact ggcaaggttg
    52201 atttgttcga ttttacgacc ttcggattcg gcgatacggg cgcgtttttc gcgttcggca
    52261 gtaatttgcg cctgcattga gcgaaggatt tcttgcggcg gaaccaagtc tttaatctca
    52321 taacgcaaaa ccttcacacc ccaagccccg gccgcctcgt ccaaagccgc aacaacagta
    52381 ctgttgattt cgtcgcgttc ttcaaacgtt ttgtccaact ccatacgccc gataacggaa
    52441 cgcagcgtcg tttgggcaag ctgggtaatt gccatgatgt agttgctcga accgtatgag
    52501 gcgagtttgg ggtcggttac ttggaaatag atgatgccgt caacagtcag ctgcgtattg
    52561 tcgcgcgtga tgcagacctg gctggggaca tccaagggaa tttctttcag cgaatgacgg
    52621 taggcgacgc ggtcgataaa gggaatcaaa atattcaaac cggccgttag ggcgcgatgg
    52681 aaacgcccga gcctttcgac aacgtggact tcctgctgtg ggatgacaac aaaggatttg
    52741 aaaccgaaaa cggcaacggc tgccaacaag ataatgaaaa attccataat tcctccgagt
    52801 gttaaggttt tgcgatgata agaaggttgc cttccttgcg gacgattagg gcgcgcgttc
    52861 ctggttcaag ctcttcttgc cccgtatttt gcgcctgcca gtgcgtaccg cgataaaaaa
    52921 cttcgtaacg gttgccgcct gtgtgtcgga ggatttcgac atattgtccg gcatccaaat
    52981 cctgatatga atccgtttca acttttctaa cggcggtttt ggcgtgtacg aaccaaatac
    53041 ccagcgcgga aagcagagcg gcggtcaaga cggcggcagg cgtgctgccg gtcagcccgt
    53101 aagcaatgcc cgaacccgcc aaagccgcgc tgacaaccaa aagataaacc gttcccgtca
    53161 ataattcgat gattaagacg gcaacagcgg caacaaacca tacagtcata catttcccca
    53221 caaagcgcgt cgtttgacaa aataacgcaa tatcagcagt atagccgaat ttgaaaggat
    53281 agggcagata tggacacttg gcacgatgca ctcggcggcg aaaaacagca gccgtatttt
    53341 caggaaattt taaatgcagt caggcaggaa cgtttgtcgg gacaaatcat ctatccgccg
    53401 gcggcggatg tattcaacgc attccgcctg acagcgttcg accgggtcaa agccgttatt
    53461 ctcggacaag acccgtatca cggggcaggg caggcgcacg gtttggcatt ttccgtcagg
    53521 cagggcgtcc gcataccccc gtctttactc aatatctaca aagaattgga aaccgacatc
    53581 gaaggttttt ccattcccgc gcacggctgt ctgacagcgt gggcggagca gggcgtattg
    53641 cttctgaaca cggttttgac ggtgcgtgca ggacaggcgc attcgcacgc ccttttaggc
    53701 tgggaacgct ttaccgatac cgtcatcagg cagcttgcga cacaccgcaa gcaccttgtc
    53761 ttcatgttgt ggggtgggta tgcacaacaa aaagggaggc tgatagacag tcaaaaccat
    53821 ttgatattga ccgcaccgca tccgtctcct ctgtcggcat atcgcggttt tttcggctgc
    53881 cgccattttt cacaggcaaa cagctatttg agccggcacg gtatcgatcc gataaactgg
    53941 aagctgtgaa tgccgatata gccgttgccg ccggcgtgtt aaaatcgcgt ttgatttgta
    54001 atttccattt attaggcaaa accttatggc aatcaaaaaa aaatcagctt tatgcatcac
    54061 tttgggcggg ctgcgacgag ttgcgcggcg gtatggatgc gagcctttat aaagaactat
    54121 gtgcttacgc tattgttttt aaaatatgtt tctgataagc ataagtacgg cggcggcatg
    54181 attgagctgc acgccgatac gacttttgac gacatcgtca aactcaaaaa caccgccgac
    54241 atcggcgacc gcctgaataa gatcatcgcc caaattgccg aagccaacga cttaaaaggc
    54301 gtgatcgacg ttgccgactt caacgacgaa gacaaactgg gtaaaggtaa ggagatgatc
    54361 gaccgtttga gcaggcttgt cggcattttt gaaaagctca acctttcttc caaccaagcc
    54421 gaagacgacg atttgttagg tgatgcctac gaatacctga tgcgccattt tgcgaccgag
    54481 tcaggcaaat ccaaagggca gttttacacg cctgccgaag tctcgcgcat tatggcgaag
    54541 attatcggaa tcagcgcaga ttgcccgtcc agcaccagcg tttatgaccc gacctgcggc
    54601 tcgggttcgc tgttgctcaa agccgccgcc caagccggca gccaaatcag cctttacggt
    54661 caggaaaaag atgtggcaac cgcgtccctt gcccgtatga atatgatttt gcacaacaac
    54721 gaaaccgccg aaatcaacac cggcaacact ttgtccgaat cgacagcgga gaagttgaag
    54781 acctgcaaaa tctcgccgcc tatctttatg gcggcatacc tgcgcacgat atggacgcat
    54841 tggaagccta ttggcaagtt ttaggccgta tgaaaaacga gttgtttgcc gaacacgatg
    54901 gccgctttac cactatacaa cggaatcgat tgcaaatctt tcccactctc aacagcttaa
    54961 aatcctgcgg gattggtgtg gaatttaggg ctaatctagt acagccccaa atttaatcca
    55021 ctataaaatc gaaagcagcc aaatcaaagc ccatatattg gcgcaccccg attacgccgc
    55081 cttcaaagcc ggacacctgg caaagtttgc cgcgtggcac actcaaaacg accttgccgc
    55141 catccaaccg ggcaggctta tccggaaatg gagcgaaagc ctgctggacg cgttcaaacc
    55201 cggcagcctg attgaagaat acgatttcta ccaaatcctg acggactact gggcggaaac
    55261 cctgcaagac gatgtttatc tcatcgccca agatggctgg aaggcggtta aaaacctggc
    55321 cgaaatcacc aaagaaagcg atgaagacgc gaacctgacc gtcgtctttg aggaaaccga
    55381 aaccggcaaa aaaggcaaag ccaaaaccaa gcgcatcagc aaaaaatacc gcagcgaagt
    55441 catcgccccc gagctggttg cccgccgcta cttttcagac ggcatcgcca agctggaaga
    55501 aaaacaaagc gagctggaac gcctaagcca agaattggaa aaccacatag aagaacacgg
    55561 cggcgaagag ggtgcgctga acgacgtatt ggatgcaaaa ggcaaacttt ccgccaaact
    55621 tctgaaaacc gcattggaag aaagcggcat agaagaaggc gaacgggctg ttttacaaac
    55681 cacccaaaca ctgatgacgc aggaaaaagc cgcgaaagac gcagtcaaaa cccaaatcga
    55741 agccctgaac cttgccgtat tcaaacaatt cggccgactt tccgaagccg aaatcaagca
    55801 gcttgccgtt caagacaaat ggcttgccga tttacaaagc cgaatcgaaa atcgcttgga
    55861 aaacagtatt cagcagctta tcagccgctt gaacacgctg gaagaccgct accgcagccc
    55921 gatggccgag cttgcccgag aagtggaaaa gtggcaaagc aaagtcaatg cccaccttga
    55981 aaatatgggt tttggaggct gaaatggcgg cacagacagg ctataaggcg agcgggtttt
    56041 gagacctttg caaaattccc caaaatcccc taaattccca ccaagacatt taggggatcg
    56101 cggttcgggt gtccgcaccg cttaatacgt cgtcgtccac gaactgaccc atttgctcga
    56161 acgccatcgc aacgcccgtt ttatggcgca tatggacaac tttctcccaa actggcaaag
    56221 catcaaacaa cagcttaatg ccttggagtt atttgcacaa acatataatt taacataata
    56281 tatattatgc gaactatcgg aaacagttgt acgtgtccct gtggtctttc caagtaggaa
    56341 aattaaagta tggacaatgc ggctgaatgt atagcccgga gcatccgcgt atccgaaggt
    56401 actgaacgat atgccctgca tccctttcga tgcttccatc cagccggtat tgggaaatac
    56461 gttgaccttc aatataatat acaggcagcc gacacacagg atatgccgct gctttttcat
    56521 catttcttct gtcaaatcct tggaacagtt gatttgaaaa cacggcttta catacgtccc
    56581 aattctcttt gcagtgcttg gatattttgc tgcctgtcca atacattgct ttgcaatgcg
    56641 ttgatttctt gatggttgat gttgccgcct tttgccagac gtgcttgtga taacattttt
    56701 tgggcttcaa ccaatgcttt gcgttcgttg ctcaattctg tttcgagaat tgagcgtctg
    56761 ctgttgtttg agggtgcttg ttgcggcggc ggcgtattgg attttgccgg cttggatact
    56821 gttttgaccg gggctttata tttgacaacc tgtccgccgt ttgacggtga tgataccggt
    56881 tcgggcgttt ggggcgggat atagcgttcg ctgctgtagt tgccgattgg gggcaaatcg
    56941 gttgagtggc agcttttgga cggcttggtg gtgtaaacgg tttctccgtt gattgtgcag
    57001 gtgtagattt tggccgcatt cgcacccaat gggcttgaaa tcagggaaaa gttgattagg
    57061 attaagggga gttttgattt cataatgtgc ttgattctcg gataattatt tgattttttg
    57121 gtatttttta taatgctttc ttggcgtttt acgggctttt cttttcggtt gtttgctttt
    57181 gattttcttt cggggctttg gcagcctgct tgttcttttt cccggcaggt tttttgtttt
    57241 tctttttact ttctatgtgt attttggctt cttaactgag ttttttaatt ttcaggcggt
    57301 atccgcctcc ctgatggctg ctgattttag gtaaatccgc atcggcgcac gatcctgctg
    57361 gggctgatgt atatacgctt ttgccatttg agttgcaatg gtatacggag gcttgcgccg
    57421 ccgcgccgga aagggacagg agacccaagg cggcaaaaag tctgatgttt ataccggtaa
    57481 aaggcggtga taacgcccga attataccgt tattggcagg caaagataag caccctgccc
    57541 gcgcttcttt atcgctcggc aaactgtttc tcgatttcag tttcaatccg ttccaattct
    57601 tcttgacaag ccagccaaac ctcttcgatt tgggcaagtt gtgttttgac ttttgccagc
    57661 tgggataagg tattttgcaa tttttctttg tttgcttcaa ggtaagcgtc ttcttgtgct
    57721 aaaaatgctt cacatgccgt ctgaatttcg gaaagctgcg ccatttcttt ttcggcacgg
    57781 tctattttct gctgtatcgg cttgccgcgt cgggcttttt cctgacggat ttgcgcttcg
    57841 atgcgcttgg tgtctttgcg gctttggctt tgtgcggatg ctgcgggtgc ggcggcggcg
    57901 ttttcctgtg ccaaacgcca ttggcggtaa tcgttcaaat cgccgtcgaa gttcttcaga
    57961 cggcctttgt cgatcaggag gaagctgtcg gtagtcgctt caagcaggct gcggtcgtgc
    58021 gatacgatga ttaaggcgcc ttggaaactt tgcagagcga gcgtcagggc gtggcgcata
    58081 tccaagtcca gatggttggt tggttcgtca agcagcagca ggttcggctt ttgccaaata
    58141 atcatggcaa gggcgagacg ggctttttct ccgccggaaa aaggttcggt tttctgcaac
    58201 gccatatcgc cggcaaagtt gaagccgccg aggaaatttc ggatttcttg ttcgcgtact
    58261 tcgggagaaa gctgctgaat atgccaaaca gggttttggt cggcgcggat ggtatcgagt
    58321 tggtgttggg caaaatagcc gatattgagt ttttcggaac ggacgatgct gccggaaagc
    58381 aaatcgattt tgcccgccaa ggctttgata aacgtagatt taccgctgcc gttgacaccc
    58441 aatagcccgt agcgtgcgcc gctttccagc gacagggtaa tgtcatgcag aacggttttt
    58501 ccttcgtaac ccaaatcagc gtgttctagc tttaacaaag gattgggcag atggtcggga
    58561 tggtaaaact caaaggaaaa ctcgctgtcc agatgcgcgg gcgcaacgcg ttctagcttt
    58621 gccaaagcct tcatgcggct ttgcgcttga acggctttgg tggctttggc tttgaagcgg
    58681 tcgataaagg attgcaaatg tttgatttgc acctgctgtt tgacataggc agcttgttgt
    58741 tgcgcgagac gctgcgcacg ttcgttttgg taaaaatcgt aattgccgcc gtattgcgtg
    58801 agtttttgct gcgataattc aatggtttgg gtagttgccg cgttgagaaa atcgcggtca
    58861 tgggaaatga tgatttgcgt gcagggtaaa gaagcaaggt ggttttccag ccacaagacg
    58921 gtttccaaat ccaagtggtt ggtcggttcg tcaagcaaga gcaaatcggc gcggcaaatc
    58981 agggcttgcg caagattcag gcgcatacgc cagccgccgg aaaaggattt gacggggcgg
    59041 ctgtgttctt cttgcgaaaa acccagcccg ttcaacaatt ttgccgcgcg ggccggcgcg
    59101 gtataagcgt cgatttcttc caatttagca tgatattccg cctgcttcat gccgtcattt
    59161 tgcgcttccg cctgcctcaa tgccgtctga aaagcctgca actcggcatc gccctgcaaa
    59221 acgtaatcca aagcggaaat atccaaatcg ggcgtttctt gggaaacgga agcgagccgc
    59281 cagtttttcg gaatcgagac atcgccgccg tcctgagtga tttcaccctt gagtaaggca
    59341 aacaggctgg atttgcccgt cccgtttttg ccgatcaaac cgacgcgctg accgggattg
    59401 acagtggcgt tggctttgtc gagcaggatt ttcaaaccgc gttgcagggt gaggtttttg
    59461 atttcaatca taacggaaac atcgtcgggc ggaaaaagcc cgtattttac ctgaaaatca
    59521 gtgccgatgc cgtctgaaac gggaaattta cggctgaagc caagcccaag ccctgcgccc
    59581 ttccgagtgc aggaaaacca atgtcctgaa tgccgaatcg gtattcatgc attccacgct
    59641 gattccgatt cgggaaaaat ccgccatgat tttgggatgg ataaactcct gagccgcgcc
    59701 cgtcccgata atcaatattt ccggatagtc aacaggtttg atgtcggaca acaggttttc
    59761 cggagtcaga tcggacaagg ttcggcattg cgacaggcag accgaatcct tatgtacaag
    59821 cacgggttta tggaaacttt gacccgccaa tcggatttca cccgaccggt ggccatactc
    59881 tgtaaaatgt ccgtctatcg gattttcttc aaacaacatt tttttacccc gtcgccgcat
    59941 catctacacc gaaaggggtg caaaatcagg cgaattcatg taggattggc agatttcatc
    60001 tgacccgcct gccgatttca gacggcattt gattcaaagt gcggcacaat tatatcggca
    60061 gcggatattt tcgtctttca atatttacat ttcagtcggc ttacaaggag acacaatgaa
    60121 gccagtaaac atcggtcttt taggtttggg tacggtcggc ggcggtacgg ctgccgtgtt
    60181 gcgggacaac gcggaggaaa tttcccgtcg cttggggcgc gaaatccgta tttctgccgt
    60241 gtgcgatttg agtgaagaaa aagcccgaca aacctgcccg tccgcagcct ttgtcaaaga
    60301 tccgttcgaa ctggtcgcac gtgaagacgt cgatgtcgtc gtcgaattgt tcggcggtac
    60361 cggcattgcc aaagatgcgg tgttgaaagc cattgaaaac ggcaaacaca tcgttaccgc
    60421 caacaaaaaa ctgctcgccg aatacggcaa cgaaatcttc ccgctggcgg aaaaacaaaa
    60481 cgtcatcgtc caatttgaag cggcagtagc gggcggtatc ccaatcatca aagccctgcg
    60541 cgaaggtttg gcggcaaaca ggattaaatc catcgccggc attattaacg gcaccagcaa
    60601 cttcatcctc tccgaaatgc gcgaaaaagg cagcgcgttt gccgatgtac tgaaagaagc
    60661 gcaggcattg ggttatgccg aagccgatcc gaccttcgac atcgaaggca acgatgcggg
    60721 ccataaaatc accatcatga gcgcactggc attcggcacg ccgatgaact tttccgcctg
    60781 ctacctcgaa ggcatcagca aactcgacag ccgcgacatc aaatacgccg aagaacttgg
    60841 ctatcgcatc aaactgttgg gcattacccg caaaaccggc aaaggcatcg agctgcgtgt
    60901 ccaccctacc ctgattcccg aaagccgcct cttggcaaac gtcaacggcg tgatgaacgc
    60961 cgtgcgcgtc aacgccgata tggttggcga aaccttatat tacggcgcgg gcgcgggcgc
    61021 attgccgacc gcttccgccg tggttgccga tatcatcgac atcgcccgcc tggttgaagc
    61081 cgataccgcc caccgcgtac cgcatctggc gttccaaccc gcgcaagtcc aagcgcaaac
    61141 catcctgcct atggacgaaa ttaccagcag ctactacctg cgcgtccaag ccaaagacga
    61201 accgggcacg ctggggcaaa tcgccgcgct gttggcacaa gaaaacgtgt ccatcgaagc
    61261 actgattcaa aaaggcgtga ttgatcagac cactgccgaa atcgtgattc tgacccacag
    61321 cacggtcgaa aaacacatca agtcggcaat cgcagccatc gaagcactgg attgcgtgga
    61381 aaaaccgatt accatgatcc gcatggaaag cctgcatgac tgagccgaaa cacgaaatgc
    61441 tgacgaaaga gcaggttgcc gcgcgcaaaa aagcaaaagc caaaatccgc accatccgca
    61501 tttgggcgtg ggtcattttg gcgttgctcg ctttaaccgc cctgctctcc caatgcgcga
    61561 tgtccaaacc gcaggcaaaa cagaaaattg tcgagtcttg cgtgaagaat attccgtttg
    61621 ccgaaaaatg gcaaaacgat ttgcgggccc gcggtttaga ttcaaacaat acccgcctcg
    61681 ccgtcgacta ctgcaaatgt atgtgggagc agcctttgga cagattgagc gagaaacaga
    61741 ttagatcctt cggcaaactc ggcgcacaag aacagcttga cctgctcggc ggcgcaaatg
    61801 cctttgaagc acgtgacaag cagtgtgttg ccgatttgaa atcagaataa tgtggaccga
    61861 taaaaaagcc gattctttaa agaatcggct tttttcataa aaaacggctt acagtgcgtc
    61921 tttcaaagct ttgccggcgc ggaatttagg cgttttggcg gcggcaatgg tcagaggctc
    61981 gccggttttg gggttgcggc cttggcgttc cgcacgttcg cccacgtaga aagtaccgaa
    62041 accgaccaaa gtaacggtgt cgccttgttt cagggcggtg gttactgcat tggtagtggc
    62101 atccaaagct ttttgtgcgg cggctttgga aatgtcggct tcttgagcaa tcgcttcgat
    62161 caattcagac ttgttcacaa tcagtccctt cctgtcttaa aaaatgatga aatgcccgaa
    62221 tactcggggt ttgtactgct tgagcaactt tcgctttata gcaattctga aattgccgtg
    62281 tcaagcaaaa aatacggaat caccctattt gacaggcttt caggacgaaa ccgcattttt
    62341 acaacacatt tcctgcgttt caatgtttgg ttgccctgct gcggggtttt ggttttgaag
    62401 cggattccgc cgccgcttcc gcaccagaag gttctgccca aggctcaggc tggctttcca
    62461 aacccagagc caatacctcg tctatccatt tgaccggatg gatggtcagg ccggttttca
    62521 cgttttcagg gatttcttcc aagtctttga ggttgtcttt tggaattaaa acgtgtttga
    62581 tgccgccgcg aagcgcagcc aacagttttt ccttcagacc gccaatcggc aggacttcgc
    62641 cgcgcagggt aatttcgccc gtcatggcaa catcggcgcg caccgggatt ttggtgaatg
    62701 cagacaccat cgccaaagtc atggcaatgc ccgcactcgg accgtctttc ggcgtcgcac
    62761 cttcgggaac atggacatgg atgtcttttt tctcgtaaaa atcaggagcc aagcccactg
    62821 attccgcgcg ggagcggaca accgaccacg ctgcagacac ggattccttc atcacatcgc
    62881 ccaactggcc ggtgcactga atcacgccct tacccggcaa tgctgcggct tcgacggtca
    62941 gcagttcgcc gccgacttct gtccacgcca gaccggttac ttgtccgata cggttttcgc
    63001 tttcggcaac gccgtaatcg aagcggcgca cacccaaata gtcgtgcaga tttttctcat
    63061 ttactttaac cgctttaggt ttggctttgc tggttttctt ggtttcagac aacctcttct
    63121 tatcttcgtc caaggtaatc tgcatcacca ccttgcggca gattttggca atctcgcggt
    63181 cgagcgaacg cacgcccgcc tcgcgggtgt agtaacggat aatatcgcgc accgcgcttt
    63241 cttcgattgc caattcccct tctttcacgc cgttgcgctt catttgcttc ggcacgaggt
    63301 actgcatcgc gatattgatt ttttcgtctt cggtgtagcc ggacagacgg atgatttcca
    63361 tacggtcgag cagcggggtc gggatattca ggctgtttga agtcgcaata aacatcacat
    63421 cgctcaaatc gtaatccact tcggcataat gatcggcgaa cttgttgttt tgctcgggat
    63481 caagcacttc gagcaacgcg ctggcgggat cgccccggaa gtcgctaccc aatttgtcga
    63541 tttcgtcgag caggaacaat gggtttttta cgccggcttt tgccatattc tgcagaatct
    63601 taccaggcat ggagccgata taggtgcggc ggtgtccccg gatttcgctt tcgtcgcgca
    63661 cgccgcccaa agccatgcgg acatatttcc gccccgttgc tttggcgatg gattcgccca
    63721 aagaggtttt gcccacgcct ggagggccga ccaggcacag aatcgggcct ttgagtttgt
    63781 ccatacgttt ttggacggcg aggtattcca aaatccgttc tttgactttt tccaggccgt
    63841 agtggtcggc atccagcacc agtccggctt tggcgatgcc tttgctgacg cgggattttt
    63901 tcttccacgg cagttcgagc agggtgtcga tgtagttgcg tacgacggtg gattcggcag
    63961 acatcggcgg catcattttg agttttttca gttcggacag gcatttttct tccgcttctt
    64021 tactcatacc cgcctttttg atgcctgctt ccaaggcatc cagttcgccg ttttcgtctt
    64081 cttcgcccaa ttctttgtgt atcgctttaa tctgttcgtt cagataatat tcgcgctggg
    64141 atttttccat ttggcgtttg acgcgtccgc gtatgcgttt ttcggcctgc ataatgtcga
    64201 gttcggattc cagctgtgcc agcaggaatt ccatccgttt gccgatttcg gaaatctcca
    64261 aaatctgttg gcgttgcgtc agtttcaact gcaaatgcgc tgcgactgta tcggttagcc
    64321 ggctgttttc ggcaatgccg ttaatgctgc cgataatttc ggcggggatt tttttattga
    64381 gtttggcgta ttgttcaaac tgcgccaaca gggtgcggcg cacggcttcg aggtcggtat
    64441 tgccgcccgt gtcttcttcc acgaccgcct ctatatggga aacgaacagc ccgcccgtgt
    64501 cttcaatagt cagaacacgt ccgcgataca gcccttcgac caatactttt accgtgccgt
    64561 cgggtagttt caacacttgc aggacttgtg cgaccgtacc ggtctgatac aggtcggcgg
    64621 caaccggttc ttctaccgcc gcatcggttt gcgccaacag gaaaaccggc tcctcgcggg
    64681 taatggcgtt ttccagtgcg gcgatggatt tcggcctgcc gacaaacagc ggcagaacca
    64741 tatgcgggta aacgacgaca tcccgcaaag gaagggttgc caaggcggca tattcctcaa
    64801 aatgcttttc tttttgtgtc ataggtactc tcttgtgtct gacagattgc cgattttcgc
    64861 gtacattggg gttgaaggta ttatttcaag catatgtggt ttatttatgg agtttgatgc
    64921 gatgccgtct gaaacattcc ggcttcagac ggcatgggct tggaaagaca aggcgggaac
    64981 aaaaaactgt tctgtgttgc cgctccttgc tgtaccatcc gtatggtttg cggttctgcc
    65041 gccctatttt caaaacatga cgcaggtatt ccatgtcttc ttttattttt cccgataacg
    65101 ggttcgaatg gcgcaacgaa agtctgcaaa agccgcccaa aggttggcat tatgtccgcg
    65161 aaagtggtgc aaacggcatt ttgaaggctg cctatcaaaa tattgcaact gtctggcagg
    65221 gcgatttcca caatgccaaa caggtgcttt ccgcaatgaa gaagaaggtc cgcaaacctg
    65281 ccgccgtccg ttccgatgcg gatattgccg cccttttcca cgcccaccgt atgaagcagg
    65341 cgcagcagag ccgtattctg aatatgcttg ccgttgaaat ccgccccggt tttgtgttgg
    65401 acaacaaacg cgcgcccgat atacgcgccg ccctgctcga cgtgtacgga gaggcggacg
    65461 gcaaaccgtt tttcctgccg ctcaatctgc tgctggggtt tatgggtgcg cacgagtggc
    65521 ataagaaagg ggttgccgtt ccgcagctgg gcagcagcat acacgttcct ttcggcgtat
    65581 tctcgccgtt gcgcggcgaa tacctcgacc tgctcgccca tgcgccgtca acgggttttc
    65641 agacggcatt cgatatcggg acgggctcgg gcgtgcttgc cgccattttg gcgaaacagg
    65701 gcattccttc cgtcatcggc acggatacca atccgaaagc cgtcgcctgc gcccgtgcca
    65761 atattgcccg tttgggcttt gaaaaacagg ttgagatacg ggaaaccgat ctgtttcccg
    65821 aagggtttgc cgacctgatt gtctgcaatc cgccctggct tcctgccaag ccaacttccg
    65881 ccgtcgaatc cgcgttatac gaccccgaat ctgcgatgct ggctgcgttt ttgcgggatg
    65941 cgccgaaaca tctgaatccc gatggagaaa tccgtctgat tatttccgac cttgccgaac
    66001 atctgcacct gcgtccgtcc gattttctgg ataaggcatt tgctcaggca gatttgcgcg
    66061 ttgcggatgt tttgaaaacc aaaccggttc acaaaaaagc tgccgacccg gacgatccgc
    66121 tggcttttgc ccgaaacagg gaaactactt tcctataccg tttgaaaaag gcataagggg
    66181 ctgcggtgcg cttccggcgg caggcatcgg aacgcccgcg ccgccggcac ggcatcattc
    66241 gggcggatta ttcttgtgaa aatacccgct cgagcatact gcccaatgcc gtctgacgcg
    66301 ttttgacagt ggcggcagct tgccggaagg tttcttctcc gccgaagaat acgaacttct
    66361 ccctcgcgcg ggtaatggcg gtatataaca gctccttact caatccggac aatgcatcgt
    66421 ccccttcgtc cgaaggtgcg gcggaaggcg gcagcagcca gacttcccga tattccgaac
    66481 cttggctttt gtggacggtc atggcgaatg cgggttcgaa atcgggcagg cagcttaccg
    66541 ctaccttttt aaatccgtcc gcatcggcaa aataggcggc aaggctgccc tgccgtccga
    66601 catcttccat aatcaatccg atgtcgccgt tgaacagttc aagcgcgtag tcgttctgtc
    66661 tgatcataat cggttctccg gcgaaatagg cctgatgttc cggtatgttc attttgcggc
    66721 gtacatggcg gcaataggct tcgttgaagt cttccgcatc ctgccgccaa gctgccagga
    66781 ccacgatatc cgagatgcct gcgtatgcgg cttcgatatt gccgtctttt accgcctgcc
    66841 aataggcttt gtgcgcccgg tacaaccttt cgactcgagc gttcggactg cattccgaat
    66901 gttccagttc gtccggaaac cggtcaaaca atgcccacgc cccctcatcg cccgatacgg
    66961 cggcacgggc aaggcagccg atgccgctgt tgtcgccgaa gcggtggctg aacgacagat
    67021 gggcggtgtt ttgcgccaac acgggcggat ttgcgctgac gctgaaaccg tgttccggaa
    67081 ggaagccggc cagcctttgg tgcgtttctc cgtccaaaac ggttttttgc gacaaaacgg
    67141 acagcaccgc cccgattccg acggacggga gctggttttc atcccccagc agaatcacgc
    67201 gcgcgccggt tttgaccgct ttcaaaagtt gcagcatcaa tgcggtatcc aacatggagg
    67261 cttcatcgac aatcagcaca tcgaacggca gcggacggat atggtcgaac gccgcctgca
    67321 ttttgggcgg gcgcagcttc agcaatcggt ggacggtttg cccttccagt ttgagcaaat
    67381 ggcggcggac ggcctccggc gcgtcaaaac cgttgattgc acggtgcagc gcgcgcgcca
    67441 tatgtgctgc cgctttgccc gtcggtgcgg caagcgcgat atggggaaga ttttcgtctt
    67501 caccgcaaat cagcgccagc agtttggcaa ccgttgtcgt tttgcccgtt cccggcccgc
    67561 cggtaatcac cataaaagac tgtaacagtg ccaaggcggc ggcatcgcgc tgtccttcgc
    67621 tgcccgcgcc ttgaaaccat tttgcgaggt tttgccgcgc acctgtcgcg tcgggggcgg
    67681 atgtgccggc tgccgccaag cgttttatct cggcagccaa atcgtattcc aactgccaca
    67741 tcctgcccaa aaacagcctt ctgccttcca aaatcaaagg cgcggcggat gttccgacca
    67801 cgggtgcgag tgccgacagc gcatcagcct cgccaccgct caaacggata aacgaatgac
    67861 cgttctgcaa tgcctgaaac aggcgttcgg tacagtttgc aagcacttcg tcgccagaac
    67921 ccgcatagtg ttccaaaaaa cggattgccg cccttgccgc cgcttgggca aattcatctg
    67981 tctgccgttc catcgtattc cttgtcgaaa tgccgtctga aggcgtgggg cttcagacgg
    68041 cgcggtgttg tttcggctgt ttaggcgttt gcgccctgtt tggggtgcag gctgccgtct
    68101 ttcatgacca tcacgcgccc gaagcggccg gcgagttcgt cgtcgtgcgt tacgaccacc
    68161 agccccgttc ccaattctgt tttcagttcc agcatcatat ccaaaacatt ccttgcgttt
    68221 gctcggtcga gattgccggt cggttcgtcg gcaagcaggc atttgggttg cgtaaccaag
    68281 gcgcgcgcaa tggcggcacg ctgccgctca ccgcctgaaa gttcgcccgc gcggtgcgtc
    68341 gaacggtgtt tcagtccgac cttgtcgagc atcgacattg ccgtttctgc cgcctcttca
    68401 cggctttttt tgccgatcag aagcggcatc atcacgtttt ccagtgccga aaattcaggc
    68461 agaagatggt ggaactggta cacgaaaccg agatggcggt tacgtaaatc gcccaaacgc
    68521 cgctggttta aggtacgcaa atcctcgccc atcagcagca ccctgccttc agacggcata
    68581 tccagcccgc ctagaatatg cagcagcgtc gattttccgc tgcccgaaga accgatgatg
    68641 ccagtgcttt cccccgcgcg gatttccaaa tccaagccgt gcagcacccg aacgtccaaa
    68701 ccgccgtccc gatagcgttt gcccacgcct tcgcatttca aaatcaactc actcataacg
    68761 caaagcctcc gccggttggg tttttgacgc gcgccggctc gggtagagcg tcgccacgaa
    68821 agacagtccc aaagaaatgc aggcaatcag ggcaacgtcg cccatatcga catcgctggg
    68881 caggtagtcg ataaaataaa cctgtgaatt gatgaggtgg acaccgagca ggttttcaaa
    68941 aaacgccacg accctgccga cgttccaacc caaaagcacg ccgcagacca cacccgccag
    69001 cgtgccgaaa aagcctgaaa acgcgccctg caccataaaa atcttcatca cgccgccagg
    69061 ggaaagaccc aaagtccgta aaatcgcaat gtccgcctgc ttttccgtaa ccgccatcac
    69121 caaggaagag acaaggttga acgccgccac ggcgataatc agcgtcagga tgatgaacat
    69181 catccgtttt tccaactcga ccgcctcaaa atagcttcga ttgctgaacg tccaatcgcg
    69241 cacccaaacc gtatcccttt gcgcctccgg aatcagtgtt gccgtcaagg cgggggcgtt
    69301 ttgcgggtcg gcgagtttca gccgcagccc cgcaacttcc ttatccaagc ggtacagtac
    69361 gcgcgcgtct tggatatgcg tcatcgccaa tgagttgtcc acttcgtaaa cgcccgtctt
    69421 aaccagaccg accacggtaa actgcttcag gcgcggcacg actccggcag gggtaacgtt
    69481 gccctccggc gtgatgacgg taactttatt gccgacttcc gcccccaaag cctccgccaa
    69541 gccgacaccg aggataatgt caaactcgcc cggaatcagg tcttcaaatt tgcctgccgg
    69601 cattttgtcg ccgtattcca ccactttgcg ctcttcagac ggcaaaatgc cgcgcatctg
    69661 cacgcccctg atttcgcccg cattggccag caatgcctga ttggaaacat agggcgcggc
    69721 agccaaaata cctttgcggt tttcggtaaa ccgaagcagg ttgcgccaat ccgtatccgt
    69781 attgtcgata tagccgattt cggcgtgcgg cgcgacattc aggagctgcc cgcgtatttc
    69841 tttctgaaag ccgttcataa ccgacaagac gacaatcagc gcggttacgc ccaaggcgat
    69901 tccggcaatc gaaaccatcg tgataaacga cataaagccg ttgcgctttt tcgccctgag
    69961 atacctcaag cctatccaag cctctagaga aaacataacg ctaccttaaa aatgtctgca
    70021 aacgtgccgc cccggacggc ggtttggggg cggcggcaaa agtttattgt accgtaaaac
    70081 ccaggcagcg tccgaacgcc cgagtgcggg ggacggggcg gcagagcggg cggaaaactt
    70141 gcacaacgcg tcaaactgcc ctatcctttc cttcagaaaa accgtttctt gaggaaaaca
    70201 atgaatatcc gaactgcttt tgctttgtgc gccatcgcct tatccgccgc tgcggctgcc
    70261 tacgccaaag aaatcaaaat cgatgccaac aacacgcctt attccgaagc cgacgcgcaa
    70321 aagctggcgg caacggcagt cggtatgggt gttaaggaac ctatcagcct gaacggcggc
    70381 agcgacagca ttaccgtgtc cggcagcagc gcgacgcagt gcgtgttcaa agtcggcaac
    70441 ggaggcgcat tgcagattca agggctaaac tgcaagtaaa ccgcccggaa aaatgccgtc
    70501 tgaaggcttc agacggcatt ttgcattggc ggcgttatgc cccgccttct ttaatcaggc
    70561 ggcgttcgta caccgcctgc gccagcgttc ccgcatcgac atattccaat tcgccgccca
    70621 aggggatgcc ctgcgacagc ctgctgactt tgtaaggcag gtttttaaaa aactcggaca
    70681 ggacatacgc cgtcgcattg ccttctgcgg taaaagcggt tgcaataatg atttcttcga
    70741 cttccccgcc gcccagccgt tgcgccagcc tgtccaatgc gatggcggat acgtccattc
    70801 ccagtgccgt attgatttgc cccatcagga cgaaatacag cccgtcgtgg cagtttgccg
    70861 cttccatatt cgacacgtcg gcaggcatat gcaccaccat cagccgccgc ccgtcgcgtg
    70921 tttcatcggc acaaatatcg cacaatccgc cttcgcaaaa cgtgttgcac atcgcgcaat
    70981 gatcgacctg cttcaatgcc gtctgcaagg catccaccag tttttcagcc tctttgcgct
    71041 tgtgttggag caaatgatac gctatccgct gtgccgattt cggcccgacg ttgggtaaaa
    71101 ccttcagcgc gtcgatcaat ccttggaagg catcttgttt tttgtggctc atcatattcc
    71161 gccgtatggg aaaacggccg gaatattccg accgttattt tgtcaacaaa agtgtcaatt
    71221 actgaccgtc gccgttgtcg accgattgcg ctcctttggt ctgtttgatt ttgccgttga
    71281 aataacgtat caacaagtcg aaagtattgg cagactgctg ttgcgccaaa gcctgttttg
    71341 caagcggaag ctgtgcggcg atatcatccg gcggggttac agcctgtact tcgacaatca
    71401 cgggtgccgg cagaccgatc agcctgatgt aggcgggttt gccgtttgcc ggttttgctt
    71461 tcagcagttc cgcataagcc tcgggcggca tggactgcct tgcctgctgt gcgcccaaaa
    71521 cggacacttc cgaccatttc acgtcaacag ccttgccgcc gttcagttgg gtaagcacgt
    71581 cttttgcctt gttttcggca agtttggcgg cttcggtacg gatataagcc tgacgtaccg
    71641 cgtctttggc ttcggcaaac ggcagggttt tctcttcgcg gacttctttg gcgcggacga
    71701 cccacgcggt ttcgctgttg atggtcagca cttcggaatt gtgttttttc ttcaatacgt
    71761 cgtcgctgaa tacggcattg atcaggtttt cgggcatacc ggacatttgc gcatcctgcc
    71821 tgctcagcca agtttcttgg gtttcgactt tcaaaccgct gtttttggcg gcttcggcaa
    71881 gcgaggaagg atggttgaac gcgtcatcgc ccagcttttc ttttgcctta ttgaagtcgg
    71941 caaccgcctt tttcattttc aattcgtttt cgacggcggc tttttcctgc tcgaaagaag
    72001 gtttggcttc atttgtcggc aaacgcgcca cgcgctcttc aaacgcattt ttcacttccg
    72061 tttcactgac ggtctgtttg tctgcaaaat ccttcagatt caaggcgaca tattctaatt
    72121 tgaccgcctg cggcagcaga tagtcttttt tgttcgcatt ataaaatttc tgcaaatcgg
    72181 cttcagacac tttgacttgg gcgatgaact cgtcggggtt gaaagtgtgc gaacggatgg
    72241 tgcggttgac ctgcgtcagc ctgatcagct gttccgcctg cgcgtcgccg accaatacgc
    72301 cgttttggac gaggtttacc aaattctgca aggcaaactg atcgcggatt tcttcgacaa
    72361 actggtcttc agacatatga cgttgggaaa ggtagcggtt taaaagcgcg tggtcgaatt
    72421 tgccgtttgc gtcgtggaaa ttgggatcgt ccacgataat ctgcttgatt tgttcggaag
    72481 aaaccgaaat gcccatcagc ttcgcgccct gtttcaggta ggcgcgttgc agcagggatt
    72541 ggaacaccgc gtcgcgcgaa gggccgccgc cgtccgcctg ttcgttctgt atggcgttgt
    72601 tgatggagtg gtcgctgatt ttttcgtcgc ccacttggac gatgtagtcg gcacccggat
    72661 gggataccgt gctgaccccg aagccgacga aggttaatgc aatcaggccc aaaaggactt
    72721 gggcgggcgt tctgtatttt tcgatggaat ggaacatatt ttaaatcggg atatagaatg
    72781 ggaacgggaa attcaagtcg ggtattgtaa cggtttttat ccctgtctgc acggggcttg
    72841 ccggttgaag atgccgtcgt aggtttcttc gctgaagccg gcgtaaacct tgccgccgca
    72901 ctccaatacg ggacgcttga tcaggctcgg catttcggac atcagtttga cggcctccgc
    72961 tgtcgaggac agggcttttt gctgtgtttc ggcatcgagt ttgcgccagc ttgtcccgcg
    73021 tttgttgagc agggtttcca aaggcacttg ttccagccac gagcagattt ccgcttcaga
    73081 cggcatctgt tttttgaaat cccgaaactc aaactccaag ccgtatccgg caagccggtt
    73141 tttggctttt ttgaccgtat cgcaatttgg gatgccgtga aggactatca tttggaaacc
    73201 ttttgtctga aataataaaa cggatatttt actataagtg tctgaaaatt tgcccgtctg
    73261 tttcagacgg cggggcggtt atgttacaat ccgaaaattc aacaaattta atctcttgtt
    73321 caatataagg ttttacccat aatgatttct accaacggca ttaccatgca gttcggcgcg
    73381 aagccgctgt ttgaaaacgt atccgtcaaa ttcggcgaag gcaaccgcta cggcttgatc
    73441 ggcgcgaacg gctcgggcaa atccaccttc atgaaaatcc tcggcggcga tttggaacag
    73501 acggccggtg aagttgcgat tgaaaacggc gtgcgtttgg gtaaattgcg ccaagaccag
    73561 tttgcctacg aagacatgcg cgtgctggac gtggtgatga tgggccatac cgaaatgtgg
    73621 gcggcgatga ccgaacgcga cgcgatttac gccaacctcg aagccaccga agacgactac
    73681 atgaaagccg ccgaactgga agccaagttc gccgaatacg acggctacac cgccgaagcg
    73741 cgcgccgccg aactgttgag cggcgtgggc atttccgaag atttgcacaa tgcgaccatg
    73801 gcagaagtcg ccccgggctt caaactgcgc gtattgctgg cgcaagccct gttctccaaa
    73861 ccggatgtat tgctcttgga cgaaccgacc aacaacttgg acatcaatac catccgctgg
    73921 ttggaaggcg tgttgaacca atacgactcc acgatgatta tcatcagcca cgaccgccac
    73981 tttttgaacg aagtctgcac tcacatggcg gatttggact acaacaccat caccatctat
    74041 ccgggcaact acgacgacta catgctcgcc tccgcccaat cgcgcgaacg cgccctgaaa
    74101 gacaatgcca aagccaaaga gaaactgcaa gagctgcaag agttcgtcgc ccgcttctct
    74161 gccaacaaat ccaaagcccg tcaggcaacc agccgtctga aacaggccga caaaatcaaa
    74221 tcggagatgg tcgaagtcaa accttccacc cgtcaaaacc cgtatatccg ttttgaagcc
    74281 gatgaaaaag ccaagctgca ccgtcaggct gtggaagttg aaaaactggc gaaacgcttt
    74341 gaaacccagt tgtttaaaaa cctgaacttc atccttgaag cgggacaacg cctcgccatc
    74401 atcggcccga acggcgcggg caaatccacc ctgctgaaac tcttggccgg cgcgtacaac
    74461 cccgaatatt cagacggcct gttaccggac gaaggcacca tcaaatgggc ggaaaaagcc
    74521 agtgtcggct actatccgca agaccatgaa aacgacttcg acgtcgatat ggacctgagc
    74581 gaatggatgc gccaatgggg gcaggaaggc gacgacgaac aagtcatccg cggcactttg
    74641 gggcgtttgc tcttcggcag taacgatgtc gtgaaaaaag tgaaggttct ctccggtggt
    74701 gaaaaaggcc gtatgcttta cggcaaactg ttgctgttga aacccaatgt cttagtcatg
    74761 gacgaaccga ccaaccatat ggacatggaa agcatcgaat ccttgaacat ggcactggaa
    74821 aaatacaacg gcacgctgat ttttgtctcc cacgaccgtc agttcgtttc ctccttggca
    74881 acccaaatca tcgaactgga cggcaaaggc ggatatgaac actacttggg cgattacgaa
    74941 agttacttgg agaaaaaagg cgtagcataa ccgccggttg gaacaatgcc gtctgaagcc
    75001 gcttcagacg gcattgttga taactttaaa ataggaagca tatgcagact tacctcgtcg
    75061 gcggtgccgt ccgcgattat cttttgggct tgcccgtcaa agaccgcgat tgggtggtcg
    75121 tcggcgcaga cgcacaaacc atgctggcgc aaggcttcca gcctgtcggc aaagattttc
    75181 ccgtgtttct ccatcccgaa acgcacgaag aatacgccct cgcccgcacc gagcgcaaaa
    75241 ccgccaaagg ttacgctggc ttcagtttcc acgccgacaa agacgttacg ctggagcagg
    75301 acttgatgcg ccgcgacctg accatcaacg cgatggcgca agatgcggac ggcaagatta
    75361 tcgacccttt cggcggacaa cgggatttgg cggcaggcat tttgcgccac gtttccccgg
    75421 cctttgccga agaccccgtc cgcatcctgc gtactgcccg ctttgccgcg cgctacggat
    75481 ttgaaatcgc cgaagaaacc atgaagctga tgcggcagat ggtggcaaac ggcgaagcgg
    75541 acgcattggt tgccgaacgc gtctggcagg aattggcgaa aggtttgatg gaaaaaaatc
    75601 cgcgcaaaat gattgaagtg ttgcgcgaat gcggcgcact caaagtcttg ctgcccgaag
    75661 tcgatgccct cttcggcgtg ccgcaacgcg ccgactacca tcccgaaatc gacagcggca
    75721 tccataccct gatgacgctg caacgcgccg ccgatatggg cttgagcctg cccgaacgct
    75781 atgccgccct gctgcacgac ttgggcaaag ccaaaacacc gtccgacatc ctgccgcgcc
    75841 accacggaca cgacctcgcc ggtgtcgaac ccatgcgcga agtcaatcag cggctgcgcg
    75901 cgccgaaaca ttgcgccgag cttgccgaat tggtttgccg ttggcacatt attttccacc
    75961 aagtcggaca gcttaaaagc caaaccattc tgaacgtttt gaaaaaaacc gacgctttca
    76021 gacgacccga acgctttcag acggcattga acgtctgcat tgccgacacg caaggccgtc
    76081 tgaaccgcga acacacgccc tacccgcaac gcgcgcactg gctcgcctta ctcgaagccg
    76141 ccaatcaggc ggattcgggc aaaatcgccg ccgaatgccg cgcgcaggga aaagcgcacc
    76201 ttatcgccga acaaatcgac cgggcgcggc tggcacaaat cgccccattg caaaaagcgt
    76261 ttcgagcggc gcaagacaaa acagaaaaac attaaaacgt ccaatgcagc cacttttata
    76321 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaga tagtacggca
    76381 aggcgaggca acgctgtact ggtttttgtt aatccactat aaagttttga ggacgatacc
    76441 caatccaagc tttgcaacag ccgccgccat atccgctata attcacgctt cagccattcc
    76501 gcccccgaca taaaatcatg accctgaaaa ccgatttatt gcctaaaatc aacaacgaag
    76561 attatcaacg cctcatcctc aaacacagtg cggaatttag cggtggcgaa atccgcctgt
    76621 tgaacgaaat cctcgaaaaa ttcaatttcg acgttgttca ggcgcaggca ttggcgcaag
    76681 ccgtcatgca gcaaatccgc ttcgacccca acgcctatca catcgacagc gatgacgaag
    76741 acaccacagg catctgccca cactgcatca atccgcccat gccgcccctg cgcgattacc
    76801 tcgtttggcg cgaaacgcgc ggataaaacg cttttgaccg ttatcttttc aatgccgtct
    76861 gaaacgccgc cgaccgttca gacggcatac ccgacaaagg gaacactatg ctgcaaaccg
    76921 acaacctaac cgctgcccag ccccagcgca tcatcactgc ccaaagcatc tcctcacaag
    76981 aagagttgct cgaacgtgcc ctgcgtccta aaacgctgga cgactatatc gggcaagaca
    77041 aagccaaaga acagcttgcc attttcatcc aagccgccaa aaaacgcggc gaagcactcg
    77101 accacgtttt gctcttcggc ccgcccggac tgggcaaaac cacactggcg cacatcatcg
    77161 ccaaagaatt gggcgtaaat ttgcgccaaa ccagcggccc cgtcctcgaa cgcgcaggcg
    77221 acctcgccgc ccttttgacc aaccttgatc cgcacgatgt attgttcatc gacgaaatcc
    77281 accgcctcag ccctgttgtc gaagaaatcc tctatcccgc gctcgaagac taccggctcg
    77341 acattatgat aggcgaagga cccgccgccc gttccgtcaa aatcgacctg ccgcccttca
    77401 cgctcatcgg cgcgaccacc cgcgccggta tgctgaccaa tccgttgcgc gaccgcttcg
    77461 gcatcgtctc ccgccttgag ttttacgaaa accgagacct taccaccatc gtcagccgtt
    77521 cggcacaact gttgcagctc gatatgtccg aagaaggcgc ggaagaaatc gccaaacgca
    77581 gccgcggtac gccgcgcatc gccaaccgcc tgttgcgacg cgtgcgcgat ttcgccgacg
    77641 tgaaaaacaa cggcacaatc gacggcggca tcgccgatgc cgctttaagt atgctggacg
    77701 tggacgcgca ggggctggac gtgatggaca ggaaatttct cgaagccgtt ttgcacaaat
    77761 tcggcggcgg cccggtcggt ttggacaatg ttgccgccgc catcggcgaa tctacagaca
    77821 ccatcgaaga cgttatcgaa ccctacctta tccaacaagg cttcctgcaa cgcaccccgc
    77881 gcggcaggat ggcgaccgaa cgcgcctacc tgcatttcgg gctgcccgtc gaaaaataac
    77941 gcaatgccgt ctgaaacaga gctaattttc agacggcatt tctatttcaa tcattggcgc
    78001 aaggttcagc ctgccgcttt tttccagttc cgccctcatc gcatcaatca ccgccttata
    78061 gtctggtttg ccgaaaatcg cagaaccggc aacaaaggta tccgcaccag ctcgggcaac
    78121 ggcggcaata ttgtcggttt tgatgccgcc gtccacttcg atggcgatgt gccgcccgct
    78181 ttgtgcttcg taccgatcca gcatcgcccg cacccggcgg attttttcaa gggtgtgcgg
    78241 gatgaagctt tgtccgccga atccggggtt gaccgacatc agcaaaacca tatccagcct
    78301 gtccaatacg ttttccaaca gatatacggg cgttgccgga ttcaacacca gccccgcctg
    78361 acagcccata tcacgaatca ggctcaagct gcggtcgata tggcggctcg cctcgggatg
    78421 gaacgtgatg attgatgctc ctgctttggc aaacgactga atcaggtcgt caacgggttc
    78481 gaccatcaga tgcacatcaa tcggcacgct tgcataaggc ttcaacgccg cgcaaaccat
    78541 agggccgaag gtcaggttcg gcacataatg gttgtccatc acgtcaaaat ggatcagatc
    78601 tgcacctgcc gcaatgacgc tttccacctc ttctccaagg cgggcaaagt ctgccgacaa
    78661 aatactgggt gcgatacggt aggcggtcat attttttcct tcaatgcctt tacgggcgga
    78721 ctcaacgcgg gaaagccccg aaacagcatt attaacaaaa gttaactgcc ataataccat
    78781 ctttcacacg gcaatcaagt atattgtccg aaatatctgg attaaaggaa aagccgtgcc
    78841 actgcccgct ccctgccgtt tcgccaaacc tgccgcctct tttttaagta tggctttgct
    78901 ttcctgccag ctttcccacg ccgccacggc ttatatcccc ctgaacgatt ttcaaccgaa
    78961 ctgcgacata cgccgactcg gactgacaca gggtcagcac aatgaactgc gtaaaatccg
    79021 cgccgccttc aaaatggcgg gcgacagggc gcgtttgaag gttatgcatt ccgaacacag
    79081 ccgccgccgg tctgtcgtcg aaatcatttc ttcggatgtt tttaatcgga acgaggcgcg
    79141 cgattatgtc gaaagccgct atttgtccgg tatggatttt gcggtggacg aattggaaat
    79201 ccaacaccgc ttcttccata tcctcacacc gcaacagcag caaatgtggc tttcttcctg
    79261 cctcaaataa tccccgaaac gctcacaacg cccgttgttt cggcagcctg cccgcccagt
    79321 cgcaggcaaa ctgccacgcg gaacgtcccg aacggctgcc ccgcgtctgc gcccactgca
    79381 atgccgccat ctgtgcggtt tcatcatacg gcacgccgaa atcttccagc caattttgca
    79441 ccgccgccag ataatcgttt tgatcgaacg gataaaaact gagccacaat ccgaatcggt
    79501 cggacaggga tattttttct tccaccgctt ctttctgatg gatttccccc cgcatccccg
    79561 tcgtaccggc attctcgtca aaatattcgg gcatcaggtg ccgtctgttg gaagtcgcgt
    79621 aaaccaaaac gttggcgcaa cgttgagaca gaccgccgtc taacgcggtt ttcaatgcct
    79681 tataggtttc atcgccgctt tcaaacgaca aatcgtcgca aaatacgata aatttttccg
    79741 gacgttcctt caaaagcgtc aacaggtaag gcaggccgat taaatcgctt ttatcgactt
    79801 cgatcaggcg caatccctta tccgcatatt cgtgtagcag ggctttgacc agcgaggatt
    79861 tgcctgttcc gcgcgcgccg ctcatcaata cattgttcgc gggtctgccg acaatgaact
    79921 gttcggtatt acgcaccagc aattcggttt gcctgccgac tcccgccagc cttaccaagg
    79981 gaaaggtgtg cggatcgggc aagtgttcca aaaaaccttt tttgcccgca ctctgccagc
    80041 ggaaggcaag cgcgttccaa tccgtatgcc cgggttcggg cggaagcacg gcatccaaac
    80101 gccgcaaaac ggcataggct ttatcgaaaa attcgttcaa ttccatctct acctcacttt
    80161 gcatatcttt gcgccatcag ccgttcgacg gtgtcgacga ttgcctgcgt attcggatcg
    80221 atttcgatgt tgatcctgtc gccgaccttt ctgctgccga acagcgtccg ttccaaagtt
    80281 tcgggaatca gatggacatt gaaacggccg tcttcgactt tgcctatggt caggctgcaa
    80341 ccgtccaagc cgacgaaccc tttggtcagg atatagggtt tgagttcatg cgggagcgaa
    80401 aaccaaaccg tgcggttgaa cccgtcccgt tcgatttcga caataggcac ggttgccata
    80461 atgtgtccgc tcatgacgtg tccgccgatt tcgtctccga aacgcgccgc ccgttcgatg
    80521 ttgacgcaat cgccttcctt cagcagcccc aaattggttt ttgccaaagt ttccgccatt
    80581 aaatcgaaac taacgcggtt tccttcgatt tcggtaatcg tcaggcagca gccgttattg
    80641 gcaaccgatg cgccgcgttg cagattgtcc gccgcctctt gcggaagctc gacgacataa
    80701 gtgtgaaatg cctccgacgg gcggtggatt gccgtcagtt ttcccaagcc ttgaacaatg
    80761 cctgtaaaca taatcctgtt ttcctgtgtc ggtaaaatgg tgcaaattgt agcatctccc
    80821 cgcgaaaaat gccgtctgaa atgccttcag acagcattat gcctccgatt cgggcaaaaa
    80881 ccgcccggta cggcttgacc tttcctttct cggtttgcgt gttcaagtcg gcaaaatgcc
    80941 cgtcaaactc gaatctgacc gaccgcgccc tttgctgctg caaccagctt cttaatgttt
    81001 tcatacccga aaacagatag ggaatcgcgc tttgcagaat ctgcgggcgg atatacataa
    81061 tgttgtagtg catcgttatc ggcgtttcca cataatacgc attgcacaac ggtgtgcgtt
    81121 tcgacacggt ttcaaacctc gccaccaaat cgcccggcag atacggcata tcgcacggca
    81181 caaccaaaag ccagtcggca gtcgccaact gcaaatcgtt ggctgcggta cacaatgccg
    81241 aaagcgggcc gaaatgctgc cactgccgtg cgtccggaaa aatatgcgga cttcttcgag
    81301 catattcttc caaattccgg ttggtgctga tggcgatatg gctgacctgc ggcctgaccc
    81361 tgtcgatgac atgatctatc agtgccttac cccccaaaag agcaagccct ttgtcctcgc
    81421 ctcccatacg gctcgcctga ccgccggcca gtatcagggc aaaagttttc attgcggatg
    81481 ctctcttgga aaagttcgag gttttcatga ttgcagtctg ccgttcccaa tgactcaaaa
    81541 tgccgtctga agcagacggc aaataaattc atattatctg aattttatca taacatgatt
    81601 taacactgaa gcggtacgga ttatgttttc cttaaccaat tctttctgag cagttgtatc
    81661 taatcccaaa gaatgatatt gtttgcatta tttagaacaa tttttcgccg agcatgatac
    81721 tgccagcccg tttttcagac ggcatcagcc tttccctacg cctgaaactc ctgaccggag
    81781 tgtgggtcgg gttggcggca ttgtctgtcg ttttgacact gctgctttct ttgcgtctgg
    81841 aaaacgcggc ctccgtcatc gaagaggcgg gcaacttgag aatgcaggca taccgtctgg
    81901 catacatggc gggtgaagga tctccccgct cccaaataga caatcaggtt gacgaatttg
    81961 aaaaaagcct gaatcgtatt tcacaaagtg atgccatcca tccgctgatt ccttcggaca
    82021 cccctcttgc ttatgatttg atacaatcca tgctgattat agattggcag gcacacatcc
    82081 tccccccgct ccagtcctac cggcgaccga ctcaggtcga tctctaccgc tttgccggaa
    82141 acatcgaact gtttttgcag gcattggaaa atgccaacga aaaaaacaca tggtggctca
    82201 ggcgttttca atgggcaatt atgttgatga cgctggtgtc gtctgtactg atgctgtttt
    82261 ggcaccagat ttgggttatc cggccgctgc aggcgttaag ggaaggtgcg gaacgcatcg
    82321 gacggaggtg tttcgatatt ccggttcccg aaggcggtac gccggaattc aaacaggtcg
    82381 ggcgttgttt caatcaaatg ggcggcaggt tgaaaatttt atatgatgat ttggaaggac
    82441 aagtcgccga gcagacacgc agtctcgaaa aacaaaatca aaacctgacc ctgctgtacc
    82501 aaactacacg ggacctgcac caatcctaca taccgcaaca ggctgcagaa cattttctaa
    82561 accgtatcct gcccgccgta ggagcagatt ccggcagagt ttgtttggac ggcggatccg
    82621 atgtttatgt ttccattcat catgcggatt gcggcacagc agcttcggat ttggggaagt
    82681 accatgagga aatcttcccc attgagtacc agaacgaaac attgggcagg ctgttgctca
    82741 gctttccaaa cggcatttct cttgatgaag acgaccgcat cctgcttcaa acactaggca
    82801 ggcaattggg cgtatcgctt gccggcgcaa aacaggagga agaaaaacgc ctgcttgcag
    82861 tattgcagga acgcaacctg attgcgcaag gattacatga cagcatcgca caagcattaa
    82921 cgttcctaaa cctacaggta cagatgctgg aaaccgcctt tgccgaaaac aaacgggagg
    82981 aagccgtaga aaacatcggc ttcatcaaaa caggcgtgca ggaatgttat gaagatgtcc
    83041 gcgaactgct gctcaacttc cgtaccaaaa tcagcaataa agaatttccc gaagccgttg
    83101 ccgacctatt cgcccgcttt acgcaacaaa ccgggataac ggtcgaaacc gcctgggaaa
    83161 acggttcgtt cctgccgcct caggaagcgc agctccaaat gatttttatc ctgcaggaaa
    83221 gcctgtccaa catccgcaaa cacgcccgcg ccacccatgt aaaattcacc ctttccgaac
    83281 acggcggacg ctttaccatg accatccaag acaacggaca aggtttcgac acggagaaaa
    83341 taggagaacc cacgggcagc catgtcggac tgcacatcat gcaggagcgt gccaaacgca
    83401 tccatgccgt tttagaaatc cgttcccaag ctcaacaggg aaccaccgtc tcattgacgg
    83461 ttgcatctga agaaagcttg aaatgactat taaaattatt ctgatagacg accataccct
    83521 cttccgcagc ggcattaaag cccttttgtc gcgccaacac ggttttgaag tcatcggcga
    83581 agccgcagac ggcctctcgg gcattaaaat gatcaaccgg ctccaacccg atgtcgtcct
    83641 gcttgacctt gatatgcccg gtatgaacgg acgcgaagca ctctcccaaa tcatcagcat
    83701 caatccgcag caggcagtga ttatgctgac cgtttccgaa gacagcgacg atttgaccga
    83761 atgtatgcgc atcggcgcgc gcggctacct gctgaaaaac atcaacgccg actttctgct
    83821 cgaaagcata cgcaaagccg ccgaaggcga taatgtattc tcgcccgaga tgaccgccaa
    83881 actcgtcaaa agcctgattt ccccccaacc tgcccaaggg acgcaggcac tctcctcact
    83941 tacccctcgt gaactggaaa tcttgggcta tctcgccgca ggacacagca acaaaatcat
    84001 cgcccgccac ctcgatcttg ccgaatccac cgtcaaagtc cacgttcaaa acctgctccg
    84061 caaactcaac ctcagcagcc gggtgcaggc cgccgtttac gccatccggc acaacgtccc
    84121 ccaacctgtg ccggaatagg cgttcagacg gcatattagg ggttttaatc cccgtacggt
    84181 cattcggata acagaccaag catgtaagtt tatgccccca taagtacgct tggcatagca
    84241 gtaatattgt tcggttcagt gttttccgtt tgcccctatc tgatactgca atatcagcta
    84301 tgccgtctga aaacgcatca tcatgatatt ttcagacggc ataataaaaa gcggaaatac
    84361 taatgcaggg taaaatgttc cattccgaat cccataaata tacaatggct tatatcgttt
    84421 aagcatgtgt aacccaccct catatcaatc aatatatagt ggattaacaa aaaccagtac
    84481 ggcgttgcct cgccttgccg tactatctgt actgtctgcg gcttcgtcgc cttgtcctga
    84541 tttttgttaa
//
LOCUS       DAXHTY010000002        52754 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXHTY010000002 DAXHTY010000000
VERSION     DAXHTY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 52754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52754
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.002"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            52..1005
                     /locus_tag="ACFNMK_000087"
     CDS             52..1005
                     /locus_tag="ACFNMK_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC6363437.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVSKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1109..1432)
                     /locus_tag="ACFNMK_000088"
     CDS             complement(1109..1432)
                     /locus_tag="ACFNMK_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363438.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1605..2966)
                     /locus_tag="ACFNMK_000089"
     CDS             complement(1605..2966)
                     /locus_tag="ACFNMK_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC6363439.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAIRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASQEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVEGT
                     DWE"
     gene            complement(3324..5024)
                     /gene="recJ"
                     /locus_tag="ACFNMK_000090"
     CDS             complement(3324..5024)
                     /gene="recJ"
                     /locus_tag="ACFNMK_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC6363440.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQTLTNCEAAAGRLADAVERKEKILIVADYDADGATACSVGMSGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDAEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5093..5809)
                     /locus_tag="ACFNMK_000091"
     CDS             complement(5093..5809)
                     /locus_tag="ACFNMK_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363441.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPVDIYYTVTEKHTDKSKLFGNILYTPPLLILDAA
                     GAVLALPAAALGAVVDAARK"
     gene            complement(5856..6362)
                     /gene="yjgA"
                     /locus_tag="ACFNMK_000092"
     CDS             complement(5856..6362)
                     /gene="yjgA"
                     /locus_tag="ACFNMK_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC6363442.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAIVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPNADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            6516..7847
                     /gene="pmbA"
                     /locus_tag="ACFNMK_000093"
     CDS             6516..7847
                     /gene="pmbA"
                     /locus_tag="ACFNMK_000093"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC6363443.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AAERTLRRLNSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8144..8347)
                     /locus_tag="ACFNMK_000094"
     CDS             complement(8144..8347)
                     /locus_tag="ACFNMK_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC6363444.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8590..8892
                     /gene="clpS"
                     /locus_tag="ACFNMK_000095"
     CDS             8590..8892
                     /gene="clpS"
                     /locus_tag="ACFNMK_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC6363445.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8894..11185
                     /gene="clpA"
                     /locus_tag="ACFNMK_000096"
     CDS             8894..11185
                     /gene="clpA"
                     /locus_tag="ACFNMK_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC6363446.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLDRDLKNMVYGQENAIDALVTAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPGLGF
                     TAKREHGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKK
                     VEAEFTPALRKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDW
                     DAAKEEAVLKFKKNKVKPEAEPV"
     gene            complement(11232..14330)
                     /locus_tag="ACFNMK_000097"
     CDS             complement(11232..14330)
                     /locus_tag="ACFNMK_000097"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC6363447.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENQLEAELVADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRSSEKITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKG
                     EDNLPVYHQRVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKVATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKHTKNVVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELRERFPEPDEIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNEIA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGT
                     DI"
     gene            complement(14760..15347)
                     /locus_tag="ACFNMK_000098"
     CDS             complement(14760..15347)
                     /locus_tag="ACFNMK_000098"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6363448.1"
                     /translation="MVWKTLGEVFDLKNGYTPSKSNKEYWENGSIPWFRMEDIRENGR
                     ILDNSLQHISKSAVKGGKLFPANSIIMATSATIGEHALITVPFLANQRFTSLSLKPEF
                     ADKLSIYFLYYYCFNLSEWCKKNTTTSSFASVDMNGFKRFPIPIPPLPEQEKIVAILD
                     KFDTLTHSISEGLPHEIALRRKQYEYYREQLLAFR"
     gene            complement(15373..15972)
                     /locus_tag="ACFNMK_000099"
     CDS             complement(15373..15972)
                     /locus_tag="ACFNMK_000099"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6363449.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGGDS"
     gene            complement(16138..17148)
                     /locus_tag="ACFNMK_000100"
     CDS             complement(16138..17148)
                     /locus_tag="ACFNMK_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HFC6363450.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(17145..18695)
                     /locus_tag="ACFNMK_000101"
     CDS             complement(17145..18695)
                     /locus_tag="ACFNMK_000101"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC6363451.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSVSKLIARLAVHGQ
                     EKVNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYN
                     KFHIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKS
                     KADFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNL
                     FYGTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADV
                     PHIAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREID
                     EVIAEIEA"
     gene            complement(18768..19772)
                     /gene="rfaD"
                     /locus_tag="ACFNMK_000102"
     CDS             complement(18768..19772)
                     /gene="rfaD"
                     /locus_tag="ACFNMK_000102"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC6363452.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEEFIRYIPFPDALKGKYQSFTQADITKLREAGYKEEFFDVKSGVDRYVKWMLENLA
                     "
     gene            complement(19813..20932)
                     /locus_tag="ACFNMK_000103"
                     /pseudo
     CDS             complement(19813..20932)
                     /locus_tag="ACFNMK_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(20934..22169)
                     /locus_tag="ACFNMK_000104"
     CDS             complement(20934..22169)
                     /locus_tag="ACFNMK_000104"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6363453.1"
                     /translation="MTISTIAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(22206..23177)
                     /gene="hldA"
                     /locus_tag="ACFNMK_000105"
     CDS             complement(22206..23177)
                     /gene="hldA"
                     /locus_tag="ACFNMK_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC6363454.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(23232..23972)
                     /gene="pyrF"
                     /locus_tag="ACFNMK_000106"
     CDS             complement(23232..23972)
                     /gene="pyrF"
                     /locus_tag="ACFNMK_000106"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC6363455.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVADNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(24498..25145)
                     /gene="adk"
                     /locus_tag="ACFNMK_000107"
     CDS             complement(24498..25145)
                     /gene="adk"
                     /locus_tag="ACFNMK_000107"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC6363456.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            25360..26199
                     /gene="htpX"
                     /locus_tag="ACFNMK_000108"
     CDS             25360..26199
                     /gene="htpX"
                     /locus_tag="ACFNMK_000108"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC6363457.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(26593..27891)
                     /locus_tag="ACFNMK_000109"
     CDS             complement(26593..27891)
                     /locus_tag="ACFNMK_000109"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC6363458.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRVADLLHPEKLR
                     EKLDVVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWSESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(27995..29146)
                     /locus_tag="ACFNMK_000110"
     CDS             complement(27995..29146)
                     /locus_tag="ACFNMK_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC6363459.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAHEAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            29481..30860
                     /gene="norM"
                     /locus_tag="ACFNMK_000111"
     CDS             29481..30860
                     /gene="norM"
                     /locus_tag="ACFNMK_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC6363460.1"
                     /translation="MLLDLNRFSFPVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTGEVGETGRQGIW
                     FGLILGIFGMVLMWATITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            31039..32079
                     /gene="murB"
                     /locus_tag="ACFNMK_000112"
     CDS             31039..32079
                     /gene="murB"
                     /locus_tag="ACFNMK_000112"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC6363461.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTEAFVELANADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKEHFVPNLGYGDLAAAVAERCAGREITAKEVS
                     DAVCAVRRRKLPDPGILGNVGSFFKNPVVGAGKAAALLQQHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDKQALVLVNKNNASANDVRQLAQHIKFTVFAQFQVELH
                     AEPNWLPASFSL"
     gene            32091..32741
                     /locus_tag="ACFNMK_000113"
     CDS             32091..32741
                     /locus_tag="ACFNMK_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC6363462.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            32796..33584
                     /locus_tag="ACFNMK_000114"
     CDS             32796..33584
                     /locus_tag="ACFNMK_000114"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6363463.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTIDLNQDSAFQSANLARDTSFWRHHANKPVWFCLLPPSSLTHYADTVKQWA
                     ELARACNVQHLIFTSSTSVYGDTARECDEIALPDPQTESARQILAAEQHLLDSGVPNI
                     DILRLGGLYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFKPGGRRLKN
                     IIEPRHPTRREFYTEEAAKLGLPAPDFAPDDSMGKIIRTVCDNS"
     gene            33644..34210
                     /locus_tag="ACFNMK_000115"
     CDS             33644..34210
                     /locus_tag="ACFNMK_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC6363464.1"
                     /translation="MSALLPIINRLILQSPDSRSELATFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            34230..35120
                     /locus_tag="ACFNMK_000116"
     CDS             34230..35120
                     /locus_tag="ACFNMK_000116"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC6363465.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIAPRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            35145..35903
                     /locus_tag="ACFNMK_000117"
     CDS             35145..35903
                     /locus_tag="ACFNMK_000117"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6363466.1"
                     /translation="MNSKISSDHTEDTMRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADHIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NRWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(35958..36623)
                     /locus_tag="ACFNMK_000118"
     CDS             complement(35958..36623)
                     /locus_tag="ACFNMK_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6363467.1"
                     /translation="MTVLDREQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYRAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(36737..37510)
                     /gene="folE2"
                     /locus_tag="ACFNMK_000119"
     CDS             complement(36737..37510)
                     /gene="folE2"
                     /locus_tag="ACFNMK_000119"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC6363468.1"
                     /translation="MNAIADVQSSRDLRNLPINQVGIKDLRFPITLKTAEGTQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQYSEALDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIQSLLDYDVCLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTADAEVGIEEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            37571..37717
                     /locus_tag="ACFNMK_000120"
     CDS             37571..37717
                     /locus_tag="ACFNMK_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363469.1"
                     /translation="MPSEKTLLAACLAKAKLYHLLLAAALIVSIFGIVIKSRLIHILQ
                     GRNR"
     gene            37714..38871
                     /locus_tag="ACFNMK_000121"
     CDS             37714..38871
                     /locus_tag="ACFNMK_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC6363470.1"
                     /translation="MKFATQTIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQVLEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHNVKLVWLETPSNPLLRLVDIKALAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGVVVAKTKELA
                     QPLHDMMVHTGAIAGPMDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFY
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(38934..39935)
                     /gene="hemB"
                     /locus_tag="ACFNMK_000122"
     CDS             complement(38934..39935)
                     /gene="hemB"
                     /locus_tag="ACFNMK_000122"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC6363471.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(40118..40402)
                     /gene="yhbY"
                     /locus_tag="ACFNMK_000123"
     CDS             complement(40118..40402)
                     /gene="yhbY"
                     /locus_tag="ACFNMK_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC6363472.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            40512..41132
                     /locus_tag="ACFNMK_000124"
     CDS             40512..41132
                     /locus_tag="ACFNMK_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC6363473.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQYLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            41196..43163
                     /gene="ftsH"
                     /locus_tag="ACFNMK_000125"
     CDS             41196..43163
                     /gene="ftsH"
                     /locus_tag="ACFNMK_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC6363474.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            43385..43816
                     /locus_tag="ACFNMK_000126"
     CDS             43385..43816
                     /locus_tag="ACFNMK_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC6363475.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            43809..44087
                     /locus_tag="ACFNMK_000127"
     CDS             43809..44087
                     /locus_tag="ACFNMK_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC6363476.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            44140..44718
                     /gene="pth"
                     /locus_tag="ACFNMK_000128"
     CDS             44140..44718
                     /gene="pth"
                     /locus_tag="ACFNMK_000128"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC6363477.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            44779..44985
                     /locus_tag="ACFNMK_000129"
     CDS             44779..44985
                     /locus_tag="ACFNMK_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC6363478.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSVEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            44982..45344
                     /locus_tag="ACFNMK_000130"
     CDS             44982..45344
                     /locus_tag="ACFNMK_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC6363479.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            45575..46990
                     /locus_tag="ACFNMK_000131"
     CDS             45575..46990
                     /locus_tag="ACFNMK_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC6363480.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSSAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            47109..47618
                     /locus_tag="ACFNMK_000132"
     CDS             47109..47618
                     /locus_tag="ACFNMK_000132"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6363481.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            47781..49163
                     /locus_tag="ACFNMK_000133"
     CDS             47781..49163
                     /locus_tag="ACFNMK_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC6363482.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPK
                     NLQDLIVELKNGEAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSSMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQNISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNQFKAVI
                     ESNPNLSWPL"
     gene            complement(49219..49974)
                     /locus_tag="ACFNMK_000134"
     CDS             complement(49219..49974)
                     /locus_tag="ACFNMK_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6363483.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(49984..50700)
                     /locus_tag="ACFNMK_000135"
     CDS             complement(49984..50700)
                     /locus_tag="ACFNMK_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6363484.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(50690..51517)
                     /locus_tag="ACFNMK_000136"
     CDS             complement(50690..51517)
                     /locus_tag="ACFNMK_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6363485.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            51802..52710
                     /locus_tag="ACFNMK_000137"
     CDS             51802..52710
                     /locus_tag="ACFNMK_000137"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC6363486.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT        12467 a        14623 c        13068 g        12596 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatcttgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggc tttctgcccg gagacctgac ccaaaaagtc
      601 gacccctacc tccgtccgct ttatgacgcg ctttacgacc tgatgggctt cgaccgcgtc
      661 agcaaactga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 aacgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctctg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa tcgggcagag aaaaacggat acggaaacca agctccgtat
     1081 ccgtttcttt atccgtccaa ccgatacatc aatcgaattt tctgcatcgg attgcctcat
     1141 cttcgcaaaa cattgccgaa gtttcgcggt tgcacgcctg acgcactttc tgccgcgcca
     1201 cgccttcatt ccgatcgctt gcctcaaacg tcttctgaaa aggcgtaacg ctgcatacat
     1261 acatggtgga atttgaaccg aaaaggtcga ttttgacggt attgctgccg ctttccaata
     1321 cggcgacacg gtgttccaac tgcgccagac gtgcttccaa atcgccgatt ctgtcggcaa
     1381 atgcaggcat ggcggcacac gcggtcaaaa tgcccaaaat acattttttc atcaattttt
     1441 ccttgtttct tgaaccctac ccacccggac atcctttttc cgagaggcag ggtcagactt
     1501 tatttacggg gatgaatccc cttacaaatc aggataatac atagaacggc actttatgct
     1561 tcatcccatg tgttgaaact tcattgctcc ttgtctgttg acccttattc ccaatccgtt
     1621 ccctcaacct tcggcttggg tttgcgcctg cgacggcggc gttttttcgc ctgtccttcg
     1681 tttttttcgt gccgggcagc agcctcgttt ttggtcattt ccgtccgttg ttcctgcgat
     1741 gccgtctgaa acgccgtcca ccactcggca agggcgcggt ccgcattgcc ggtttcggcg
     1801 cgcaagagca ggaaatcata ggcggcacgg aaacgcgcct gtgcaaacag tttatgcgga
     1861 cgtgcgcctt tgcggttctc aaactgcggc tgaaacatcc aaatttcgcg catcgtggcg
     1921 gaaaagcgtt gcggcacgcc ccaaccgcgt tcgacggttt cacgcatcgt attgattgca
     1981 tcggacaggg cgggcgcagg tttcaaaccc tgttgcagat tgcttttcca atggcgttcc
     2041 aactcgggcc acatcagagc cgccagtaca aaaccgaccg aaaccgattt gtcagcacgc
     2101 agccgctcat cggtattttt cagggcaagc accgtcattt ttccggcgat gccgtctgaa
     2161 acgcgcaagg cattgagcag cggatggatg tcgtccggta tgtcaaatcc gttcaaacgt
     2221 ttcagacact cgcgcgcgtg ccctgaaaac agcagtttca tgatttcgtc aaacagcctc
     2281 gctaccggtt cgtgcttcag acggcatatc gattcggcaa tcggtgcggt ggtttcttcc
     2341 gacagctcaa agcccaattt gcccgacagg cggatggcgc gcaaaatcct gacagggtct
     2401 tcctgatagc gttcggcggc atcaccaatc ataaccagcc tgcgggcggc aacatcggca
     2461 atcccgttgt ggaaatccaa aatctcttct ttttcaggat cgtaatacaa ggcattgcag
     2521 gtaaaatcgc gccgcatcgc atcttcttcg atgctgccgt aggtgttgtc tttcatgatt
     2581 ctgccgtgcg cgttctgatg aaccttcgca ccgccgcgaa acgtcgttac ttcgataatc
     2641 tccgcaccgt tcatcacatg gacgatttga aaacgcctgc cgatgatgcg gctgcggcgg
     2701 aagagtttgt gcacctgttc gggcgtggca tcggttgcca catcgaaatc cttgggttcg
     2761 atgccgagca gcagatccct gatcgcgccg ccgaccacat aagcctgaaa ccccgcccct
     2821 ttcagacggc gtatgacgtt ttcggcggca aagctcaaca tttcggcacg gatgttgtgt
     2881 ctttcggcgg gaatgaccgt tttactttcc gcttttttac tgctccgacc ggaaggcagc
     2941 atcttattca accatttttt cagcataaat attctgaccc gaagagcggc atctccacgg
     3001 gcaaccgtgt tcagacggca tcaggcggta cgattccgca agatgccgta accataaaca
     3061 aaaggcgtag aaactacgcc gtccgaatcc caccgccctc gcggcggcaa cgcgccatta
     3121 taacagattg ccctttaaaa aaatcagacc ctgcttttgt ggcggagtct gaaatttgat
     3181 gcaccttata tattgcgtcc ctaagaaggg atgattaaca aaaattaacg ccctttactt
     3241 tcttttccga ttgggaacat tcccgtcgta caaacaggca aatgaaggaa cagaaatcac
     3301 attcaaacgg cgttccgcca cctctacgcc gcttcccagt agtcgatata aagctgcaat
     3361 tcgagattgt tgcgccattc gttggcaacg gggcggtaaa ccgtgcggat gtattcggga
     3421 atgtcttcgc tgcaacgcca aaacatggct tcaaattcgc agccgtcttt ttgcagccag
     3481 acttttttgt gtttgccctc cgcgcccaaa ggttgctggc ggacgacgtg gaactcgtcg
     3541 gtaaagctcg gcggcgcgaa gccctgcccc caaacgtggc gggcgaggtt ttgcgcctgt
     3601 tccaacgtga tgtcgcatgc aggcaggctg ccgtcggtga tgaaagtttg cgacaaatcg
     3661 tcttcgcaca ccatctcgcg cacggcttct tcaaaggccg tctgaaacgc aggaatattg
     3721 tgttcgagta tgctcaaacc tgccgccatc gcgtgtccgc cgaatttcaa aatcaaatcg
     3781 ggatggcgtt tggacaccaa gtccaaagca tcgcgcaggt gcaaattggg aatggagcgt
     3841 cccgaaccgc gcacttcgcc gttgtcggca ggcgcaaaca cgatggtcgg acgataaaaa
     3901 cggtctttga ggcggctggc aacgatgccg accacgcctt gatggaagtc gtcgcgatac
     3961 gccaccaaag tcgtctgacc tgaaggcagg gtttcaggaa aagcattcag cgcgtcttgc
     4021 agcatagact gctcgatttc gcgacgttcg atattgaggt tgtttaactg cgccgccagt
     4081 tcctgcgcct cggcatcatc tcgcgccaac agacaggcaa taccgaccga catatcgtcc
     4141 agccgtccgg cggcgttgat gcgcggcccc aacgcaaaac ccatatcaaa cggctgcgcc
     4201 ttgcgccaat cgcgccgcgc cacttcaaac aaggcgcgga taccggggcg cattttgccg
     4261 gagcgcatcc gtttcaaacc ttgcgacacg aggatgcggt tgttgtggtc gagagggacg
     4321 acatcggcaa ccgtgccgag tgcgaccaaa tccaaaagtt cgcccaaatt cggctctttg
     4381 atgccgtctg aaaaataatt gcggcggcgc aattcggcac gcaacgccat caatacataa
     4441 aaaatcacgc ccacgcccgc caagcttttg cttggaaaac cgcagccttt ttggttcgga
     4501 ttgacgatga tgcagtcggg cacggtctcg gcaggcagat ggtggtcggt aacgatgaca
     4561 tccaaaccca gagcctgcgc cctcgccaca cctgcgatgc tggcgatacc gttatcaacc
     4621 gtaatcagca aatccacacc ttgctctgcc gcgatttcag ccagctcggg cgttaagccg
     4681 tagccgtgct caaagcggtt gggcacaagg aaatccactt tcgcccccat cgccgccaaa
     4741 ccgctcatac cgacggaaca cgccgtcgcg ccgtcggcat catagtcggc gacaatcagg
     4801 attttttcct tgcgttcaac cgcatccgcc aaacggccgg cggcggcttc gcagtttgtc
     4861 agcgtttgat aaggcaggag ggaagcgagt ttgtcgtcca attcggcagg actttgcaca
     4921 ccgcgcgagg cacaaagccg ggcgattaaa ggatcggcac cggcggtgag caaatgatta
     4981 aaaacgtcgg tattgacgga tcgggtttgg attttgactg acatgacgaa gttttttcaa
     5041 gatatgatga ggctgaaatg ccgtctgaaa gcttttcaga cggcattgct tttcatttgc
     5101 gggcggcatc cacgaccgcg cccagagccg ccgcaggcaa agccagtacc gcacccgccg
     5161 catccagtat caacaagggg ggcgtatata agatatttcc aaacagcttg gatttgtcgg
     5221 tatgtttttc agtaaccgtg taataaatat cgacaggcac actctgttca aaatggtaat
     5281 cggcgttcag tttttgcggt gtggcgtagt atttgccttt ggcggatacg cagcgcgtgt
     5341 aaatagtccg attgtcaagt tcgaccgctt caaactcaag ctgtttcagc ttggcgatgt
     5401 cagcaggcct gtcggtatcg tagcgcaggc aaaggccttc ggtactgaaa ttctggctgc
     5461 caggcgattc gagtttgacc ggcagggctt ggtggcgagc atagctcggg gtatcctcaa
     5521 ctatttggaa gggtttgtcc aacccggcct tcaaaatgcc cgtcagcttc gccgaatctt
     5581 cgggattgac gacgaaccag tatttcccgc ccatcatcac cagactgccc ttttccaatt
     5641 gggcattgtc tttggcaacc acaccgaagg cgcggatttg atccttgtca acgtgtttat
     5701 aggcggtcgt ttggctgaac gggtcgttca tgccccacat cattgccgta cagccgttca
     5761 acatcaaggc tgccgccaaa acggcggcgg tcgttttacg gaacaacatt ttatcctcca
     5821 atctaaataa cggcggatgt cgtctgaaaa tatgcctaaa tttccgcgtc cccgttttcc
     5881 ataacggttt tcaactcttg aaacagggcg cggaaatttt tcggcggttt gttctgttcc
     5941 tgttcttttt tggtattgcg gatgagcgtc ctcagcttgc ccgcatcggc gtttggaaaa
     6001 tcggacataa actgcgtcag cgcgccgtcg tccgccagca gctccatccg cgtccgctcc
     6061 acacgttgca aaaaggcgtt gtgcgccgca tcgtcgccgc gcagcttggc aagaaatgcc
     6121 tcgatgggcg cgggatcggt atcgcgcatc agtctgccga taaattgcgc ctgccgcttg
     6181 agcgcgccgt tggatgtgat ttttttatag gtaacgatgg cttcgtacaa atcttcgtcc
     6241 aagccgattt ttttcaacgt gtcgcttgag agcttggtca gctccatacc caaatcctgc
     6301 aaaccgttca tctgcttttt catttgggtt ttgctgaccc attcgtcttc ttgttcaaac
     6361 atcttcattc tcaaatttta tccgacgggc gcattttaac agaatgattc cgcgttcaga
     6421 cggcattttg caccgtccgc cctgcaaacg gacgcttttg cccctgccgc ccgaaacggt
     6481 taaaatgccg caacgctcca ccaagaaaga tttctatgct gttcaaccac accgcttccg
     6541 aacttctcga cctctgccgc cgcacgctcg acttggcaaa agcagcgggc gcgaccgccg
     6601 ccgaagccga tttcagcgaa tccatcggac aaagcgtcag cgtgcggctg ggcgaaatcg
     6661 aacaaatcga gttccagcag gacaagtcgc tggacattac cgtttacgtc ggcaaacgca
     6721 aaggccgcgc tggcaccgcc gatttctccg aacaagccct gcgcgacacc gtccgcgccg
     6781 ccgccgacat cgcccgccac accgccgaag acggttgcgc cggacttgcc gatgcctgtc
     6841 tgatggcaaa acacatcggc gaccccgacc tttaccacga atgggatttg gatacggaag
     6901 ccgccgtcgg cttggcaaaa caatgcgaac aagccgccct gaacgaggat gagcgcatcg
     6961 aaaactccga aggcgcggcg gtgcaaaccg gccattacca atacgtttac ggcaacaccc
     7021 acggttttgc cgcacaccgg caaggcacgc accacagcat ttcctgcagc gtcgttgccg
     7081 ccgacgaaaa cggcatgcag cgcgactact ggtacgattc cgcctgccgc catccggata
     7141 tggacagccc cgaaaccatc ggtcaaaccg ccgccgaacg taccttacgt cgtctgaaca
     7201 gccgcagcat cccgacaggc agctaccccg tcctcttcga taccaccgtt tcgggcggtc
     7261 ttatcggaca cctcgtcggc gcactctccg gcggcgcgct ctaccgccaa agcagtttcc
     7321 tgatcgacag catcggcaaa aaagtcctgc ccgatttcct caacctgcgc gaagaaccgc
     7381 acatcccccg ctctttccgc agcagctatt ttgatgcgga aggcgttgcc accgcgccgc
     7441 gcttcgtgat tcaaaacggc attgtcgaag gctatttcct cagcagttac agcgcgcgca
     7501 aactcggtat gcagaccacg ggcaacgccg gcggcgcgca caacctgtat ttgaaccata
     7561 cgcacgaaac gcaatccgac ctgctgaaag aaatggggac gggattgttg gtaaccgaac
     7621 tgatgggaca gggcgcgaac accattaccg gtgactactc gcgcggcgcg gcgggttttt
     7681 gggtggaaaa cggcgtgatt gcctaccccg tccacgagat taccgtagcc ggacgcttgc
     7741 aggatatgta ccgcgacatt gtcggcgtgg cggatgacgc tttgcggcgt tcgtccaaca
     7801 aaatcggctc gattctgatt gcggggatga cggttgccgg aagctgaacg ccctgccgat
     7861 gccgtctgaa gacgaaggca gcacgttttg ccccgtgcct gcccgtttgc cgttttatgc
     7921 cgcaccgcct tttgccgaca acattgcaca ttttttcatc agcactgatt ttcccttatc
     7981 cgtcaaaata ctaagccact tgtccacaac agggtacaca caagttcagg ataaggcgtg
     8041 cggggattgg ggtttttccg ccgccggcaa aacggcagac aaaaacggcg ggacattgcc
     8101 tgtcccgccg ttttcaaata tacatttgac cgtacatcag gaattaagca gcctgaatgt
     8161 tggcggcctg tttgccttta gggccggtgg ttacgtcgaa agagacacgt tggccttctt
     8221 tcagggtttt gaaaccttcc atattgattg ctgagaagtg agcgaacaaa tcttcgccgc
     8281 cttcatcagg cgtgatgaaa ccaaaacctt tagcgtcgtt aaaccatttt acgataccgg
     8341 ttgccattag aaacttccta tactcaaaaa ttaacaaaat cagcaaaaca aggcatacgg
     8401 caaaccgagc cgtctggcgt tccttccagc ttgatatact cggcggatgc ccaatcctgc
     8461 ttaaccgtct ttttacattt gttaagacaa aacgtcaagc tatgttttca aaaaagtggg
     8521 aaaataggca aaagtaccga aaatacaaca aaaatgccgt ctgaacgcgg ctttcaaatg
     8581 ggacacacta tgaccgcgca acaccaatcc gacacgctct tgcaccgtct gaacaccctg
     8641 ccgccgaaac gttacggcgt tttcctattg aacgacgatt acaccacgat ggaatttgtc
     8701 gttgaaatcc tgaccgaaat cttcatgctc ggacaagaac aggcggtagc ggtaatgctc
     8761 ttggttcatc acgaaggcaa aggcctgtgc ggcacttaca cgcgcgatat tgcccaaacc
     8821 aaacagcaac aagtcatgca gcgggcaaaa gccgaagggc atccgctgca atgtattgtc
     8881 gaggagattt aatatgcttg cacccgaatt ggaacagatt ttgcagcagc tttaccgcga
     8941 ggcgcgtaag gctcattatg aatttatcag cctcgagcat ctgcttttgg tactcatcga
     9001 agaagatgcc tccgtgccga acgtcttaaa actctgcggc gcggatttga aagtggtgtc
     9061 cgaacagctc gccgccagcg ttgccgaaaa cacccccctg attcccgaac accttttaga
     9121 cacggtcgaa acccggccca cgctcggctt ccaacgcgtg atgcaacggg cgatggtgca
     9181 tacccagtct gccggaaaag ccgcagtcga accgttggac gttttggtcg cgctgatgag
     9241 cgaaaccgac agccacaccg tctatttcct caagctgcaa tcggttacgc gttttgaagt
     9301 tttgcgctgt attgcccacg gctctcccga tgaagatgaa gacgatggca actattcttc
     9361 agacggcatg gacgacgata atgaaaaccg caccaaaccg ggcaaaaacc ctttatcggc
     9421 gtacaccgtc aacctcaacg ccgaagtcaa agccggccgt atcgaccctt tgattggtcg
     9481 caaacacgaa atggaacggc tggtgcaaat cctatgccgc cgccgcaaaa acaatccgct
     9541 tttggtcggc gaagcgggcg tgggcaaaac cgcgctggcg gaagggctgg cacatcaaat
     9601 cgtcaacggc ggcattccag acgcgctcaa agatgccgaa gtgtacgcgc tggatatggg
     9661 ctcgctgttg gcgggcacga aataccgcgg cgactttgaa gcgcgggtca aatccgtctt
     9721 gaaacagctc gaaaaaatcc cgcacgccat tttgtttatc gacgaaatcc acaccatcat
     9781 cggcgcaggc agcaccagcg gcggcaccat ggacgcgtcc aacctgctca aacccgcgct
     9841 ggcaaaaggt tcgctgcgct gcatcggcgc gaccacttac gacgaatacc gcaccatttt
     9901 cgacaaagac cacgccttaa gccgccgctt ccaaaaaatc gacgtggtcg aacccaccgt
     9961 ttccgaaacc gtacaaatcc tgcgcggctt gaagccgatg tttgaagcct tccaccaagt
    10021 ccgctatacg caaggcgcac tcgaagccgc cgccgaactc tccgcacgct acatcaacga
    10081 gcgtttcctg cccgacaaag ccatcgacgt gatggacgaa gcaggcgcgg cgcaacggat
    10141 tctgcccaaa tccaaacaga aaaaagtcat cggcaaagca caaattgaaa ccgtcatcgc
    10201 caaagtcgcg cggattcccg aaaaaaccgt gtcgcacgac gacaaacaag tgttgcaatt
    10261 cctcgaccgc gatttgaaaa acatggttta cggtcaggaa aacgccatcg acgcgttggt
    10321 taccgccgtc aaaatgtcgc gctccggcct tgccctgccc gacaaaccga taggcagttt
    10381 cctcttctcc ggtccgaccg gcgtgggcaa aaccgaagca gccaaacagc ttgcctactc
    10441 gatgggcgta ccgctgcaac gctttgatat gtccgaatac atggaacgcc atgccgtatc
    10501 acgactcatc ggcgcgcctc cgggctacgt cggctttgaa caaggcggcc ttttgaccga
    10561 agccgtcaac aaacagccgc attgcgtgtt gctcttggac gaaatcgaaa aagcccaccc
    10621 cgacattttc aacgtcctcc tgcaagtcat ggacgcaggc aaactgaccg acaacaacgg
    10681 caagagtgcc gatttccgca acgtcatcct aatcatgacc accaacgcgg gcgcggaaag
    10741 cctcagccgc cctggcctcg gctttaccgc caagcgcgaa cacggcgacg aaatgcaggc
    10801 aatcaacaag ctcttcacgc ccgagttccg caaccgcttg gacgcgatta tcccatttgc
    10861 acccttgtcc gaacccatca tcgtcaaagt cgtggacaaa ttcctgcttc agctcgaaca
    10921 ccagctcctc gacaaaaaag tcgaagccga attcacgccg gcattgcgca aatatctggc
    10981 ggaaaaaggt tttgacccgc aaatgggtgc gcgcccgatg caccgcctga ttcaggaaaa
    11041 aatccgcaaa ccgctcgccg acgaactgct gttcggcaaa ctcgccgacg gcggctttgt
    11101 gcggatagac tgggatgctg caaaagaaga agccgtgttg aaattcaaga aaaacaaagt
    11161 caaacctgaa gcagaacctg tttaaccagt tgaaaaataa aaagccgtct gaaatttcag
    11221 acggcatttt gtcaaatatc ggtccctatt ccggcaaact tctccacaaa cgccgcaatc
    11281 ttttgaaaaa cactttgttt cttcgtcaga tattgcgggt tgagcggact cattttcggc
    11341 agggtttcgg tcagttccgt accgttttcg ctggcatagc cgcgtttgag cgagccggtc
    11401 aaatagcgtt tggcggcgat ttcgttcaga ccttcggcgg caatcaatcc tgccgcttcg
    11461 tgccgcatta cctcttgcgc gtaggtgtaa aaggtttcca gtatggcggg aacgtcgggc
    11521 actttgtcca aatccgtatc gttgatgaaa tccacaatca gaccctcttt ggcgcggtgg
    11581 ccgatgctgg cgcggatgat gcggcggatt tcttccacca gctccgcttt gcctttgatt
    11641 tttttgtggt gttcgaaaac cagttgcaag atgtaatcca gattgatttc ctgtgatttg
    11701 agcaaatcca cctcaaaaac cacatcgtcc caatcgattt ttgactgttc tttctgttcg
    11761 cctgcttttt ggcggcgcaa ccaatcgcgg atgtcgttgt aggcggaacg gtagtcctgc
    11821 accgcccttt cagacggcat cggcacttgc cgcatttcct gcacgtcttc atcgctcaag
    11881 tagtattttt cttgaaacgc cttcatcgca tcttcgtccg ccgcgtccac actctgcaac
    11941 tcgcgcagcg cggcaaattc atcgtagttc tgcaatacgt tttccgcccg caggtattcg
    12001 ccgaagagtt tggcaaaatc ttttttgtct ttttccgttt cgatttcgtc gggttcgggg
    12061 aaacgttcac gcaactcttt tgccacatcc aaataaccgc gccgcgcttc gccggtctgg
    12121 ctgtcggtat agccgttcat gtattcttcg tagctttttt ccagcaccac gtttttggtg
    12181 tgtttgtcgc cgaacagggt aatcgcatca atggttgcct gctccaaatc gcggaagcag
    12241 acaatgttgc cgaaggtttt ggtggcatcg taaatgcggt tggtgcgcga aaacgcctgc
    12301 atcaggccgt gatagcgcag atttttatcg acaaacagcg tattcagcgt cggcgcgtca
    12361 aaacccgtca aaaacatgcc gaccacaatc agcaaatcta cttcctgatt tttcacccgt
    12421 tttgccaaat cgcggtagta gttctgaaag gctttgctat ccgtgccgaa attggttttg
    12481 aaacaggcgt tgtaatcgtt gatggcagcc tgcaaaaatt ctttcgcgct gctgtccatc
    12541 gcttccggtt cgaaggtctc atcgacaatt tcaccgacgg cgttttgctc ttcgttggcc
    12601 gcaaaggaaa aaatagtggc cactttcaac gggtgcaggc tgcctgcctg ttgtgttttg
    12661 aacgcttcgt aatagcactt tgccgcatcc acgctgctga cggcaaacat ggcgttgaag
    12721 cctttgccgc ccgcattcag gcggtgcgtt ttctgcctga actgattcag gatatattgc
    12781 gtgatttcgc ggatgcgttc agggtgcagc agggcttggc ggttttcggc ggcactcagt
    12841 ttcttctcgt cctgttccgc ttccacggct ttgaactgcg ggcgtacatc gttgtaatcc
    12901 actttgaatt tcaatacttt ttcatcgcgg atggcatcgg taatcacata agaatgcagc
    12961 tcccgcccga acacgcccgc cgtggtttcc gcgcccaaag cattttcagg aaaaatcggc
    13021 gtaccggtaa agccgaactg gcagaatttc ttgaatttct ttttcaggtt tttttgcgct
    13081 tcgccgaatt gcgagcggtg gcattcgtcg aaaataaaga caactcgctg atggtaaacc
    13141 ggcagattat cttcgccctt catcaggttg ttcagctttt ggatggtggt aacgatgatt
    13201 ttgttgtcgt ctttttccaa attgcgtttc aagcctgccg tgctttccga accgttcacg
    13261 ctgtcgggcg aaaaacgttg gtattccttc atcgtctgat agtccaaatc cttcctgtcc
    13321 accacgaaga aaaccttgtc gataaacgcc gattccgtcg ccagacgcgc cgccttaaag
    13381 ctggtcagcg ttttgccgct gcccgtggta tgccaaacat agccgccgct ttccggcccg
    13441 ctccaattct tcgcctgcgc cgaactgttg attttccaca aaatacgttc ggcggcggca
    13501 atctgatacg gacgcataat cagcagcgta tcattcgcat cgaacacgct gtaatgcagc
    13561 aaaacgctca gcaatacgct tttctgcagg aacgtggcgg taaagtcttt caaatcctta
    13621 atcggatgat tgtccgaccg cgcccaattc atcgtgaaat caaagctgtt tttgtcgcgc
    13681 ttggtagtgt tggcgaaata gcgcgtgtcc gtgccgttgg aaatcacgaa gatttgcagg
    13741 aatttgaaca gcgaattttc gctgttgaag ctctctttgc tgtaacgatg cacctgattg
    13801 aatgcttcgc gcacagccac gccgcgcttt ttcaattcaa tctgcaccag cggcaggccg
    13861 tttaccaaca cggtaacgtc atagcggttt gcatgcgtgc ccgtctgctc aaactggttg
    13921 ataagctgca catggttgcg ggcaaggttt ttcttgtcca gcagataaat gtttttcaga
    13981 cggccgtcat caaaagcgaa atcgtaaata tggtcgtcgt ggattttgcg ggttttatcg
    14041 gtaatttttt cagacgacct gtccagatac tccgtcaaaa aacgcgccca ttcgccgtct
    14101 gaaaacgcca catcgttcag ccgctgcaac tgcgcgcgca ggttttccag cagcctgctt
    14161 tggctgttca aatccttgcg gtattcgtaa ccctgattct gcaaatcggc gactaactcc
    14221 gcttccaact ggttttccga ttggtagctg cccgactgtt cgattttttc atattggtcg
    14281 agcacgatga aattcggcgt ttcggcgatg ggtttggttt cgaggttcat agttcgtcct
    14341 ttgcagtgtc gggtgttaaa cggtttaatg ttcggattgg gctgcctgaa aatgtgcagg
    14401 ctgctttggt tggcttcggc ggaatgtggg gcggttgtgt ccgccgtagg gtgggcttca
    14461 gcccgccgcc ccgcctgccg ttgttttact gttgttgcag cttgccgttg tttcggtggg
    14521 ctgaagccca ccctactttg tctgcgactt ctaccccacc gatgccgtct gaatcatccc
    14581 aatcgggttt ttctctttgt tttgagcatc tatcgccata ttttccggtt tccacacgct
    14641 gcggcggaat gcggggcggt tgccgctcac cgtagggtgg gcttcagccc accgccccgc
    14701 ctgcccttgt tttactgttg ttgcagattg ccgttttttt cggtgggctg aagcccaccc
    14761 taccgaaagg caagcagctg ttcgcggtaa tattcgtatt gtttgcggcg cagggcgatt
    14821 tcgtgcggca ggccttcgct gatggagtgg gtcagggtat cgaatttgtc gaggatggcg
    14881 acgatttttt cttgttcggg gagtggaggg atggggattg ggaagcgttt aaaaccattc
    14941 atatccacag aagcaaaact cgatgttgtc gtattctttt tgcaccattc actaagatta
    15001 aaacaatagt aataaagaaa ataaatgctg agtttatcgg caaattcagg ctttaaagat
    15061 aaactggtaa atctttggtt ggctaagaat ggaacggtta ttagtgcatg ctcacctatg
    15121 gttgcagatg tagccatgat aatagaattg gctggaaata gcttaccgcc ctttactgcg
    15181 ctttttgata tatgctgtaa tgaattatct aaaatacgac cattttccct aatatcttcc
    15241 attctaaacc aagggattga gccattttcc caatattctt tatttgattt ggaaggggta
    15301 tagccattct tcaagtcaaa cacctccccc aacgtcttcc aaaccacatc tttcagacgg
    15361 cattgatagc catcagctat cccccccccc gatttgattg tcaaaatcta aaagaaagtc
    15421 gcggtaatac cggtattggc gtttgcgcag ggctaattca gcttccagct cggtgaattt
    15481 gtcaagtatt tttacaattt tttgttgggt ttccaggggt gggataggga ttaataattt
    15541 tttcaagtta ctggcattta gagcaggaat acctgtaaat gtacataagg catgaatctc
    15601 ttgttcaaat tcaagtaata tatgatagag aaaacgaaca tccaattctg ttctattctt
    15661 aatagtaact gcataattta agttgccacg aaaatatctt ccttcttgcc acgttattga
    15721 gccgactccc gctcctcttg ttgtgatgcc gatagggtca ttttcagtgt tccattcatc
    15781 aatatagcca agcggctctt tgccactgtt aataactgga taactaccaa tattatttga
    15841 gattttctgc ttacttacag cttgtcctgt ttttatgata gctatcccat tttctccacc
    15901 caacggcttc cactccaccg gtgccgtctg aatcatctca atcaattttt tcgctttgct
    15961 ttgcatatcc atggttgttt tccgtttctt tcccgctgtg gcgggatgtg gggcggttgt
    16021 ggggtgcttc ggctcactct gtccgtttgc cgttatttct gcgggctgaa gtccgtcctg
    16081 ctttcaggct gcctgcaatg gtgtgcaggc tgctttttca ggtagcctga atggtagtta
    16141 ttttgtgcgg tcttttttct gtttgatctg ttgcacggct gttttttcca acgctttaac
    16201 gctttccaga taggcttgct ccaccggcga caggatgcgc gcttcatagg cgcggtattc
    16261 gcgttcggct ttctgctctg cctgtttgcg gctgatgctg cctgcaccct gtaatgtgcc
    16321 ttgtccgtac agcccggaaa atttgtccaa ttcggctatc cagtcgcgca tatacatggg
    16381 gctttgctcc tgcgctttga tttccgctag gtcgaagaag gcggaaacca gacggttcag
    16441 gcggaacagt tcgtcttcgg tcagatagtt tttggcgatt ttggcttcat tcagcgtggg
    16501 gattgcgcct tgaaaggtgg tcagccccat aaagtctttg ctgctgtcgg cacggctgta
    16561 tatcagctca gctgcggttt gccggctggc ggcatagtgc agtttgtttt gaacggcggc
    16621 aaaaaaggtt tggctttcgc tgcttttggg gttgtagtct tggctggtgg catataaatc
    16681 aagcacttgc cggtataggg ctttttcgct gctgcggatg tcgcgaatgc ggttgagcag
    16741 ttctttccaa tagtcgccac cgcctgtgcc tttcaggcgt tcgtcgtcta tggcaaagcc
    16801 tttggtcaga tattcgtcca gccgttcggt tgcccattgg cggaattgga tgccccgcgc
    16861 ggaacggacg cggtagccga cggcaataat catgggcagg gaataatggg cgattttgcg
    16921 gtttaccttg cgcccgtttt cattttgaac tgtcaactgg aagttgacag ttgccttctc
    16981 ttccaattct tgctctgcaa gaatggtttt aatgtgtttg ctgatatttt gtttggtggt
    17041 ttggtacagt tctgccatgt ccgcctgcgt cagccaaagc tgtccgccaa attcctgcaa
    17101 ggcaaattgg gcagtgccgt cgttggcggt gtataggatg atgctcatgc ttcaatctct
    17161 gcaatcactt cgtcaatttc acgccgcagc cgttcgattt tggcaacggt ttcgctgatt
    17221 tcggcgttaa gctgtttgat gtcgataatt tcgcgggtgt cttcgggttc aacatagctg
    17281 ctgacggcga ggttgtagcc gttgtctttg acggtttgct gggcggcgtt ttgggcgata
    17341 tgcggcacat cggctttatc ggcgaagagt ttgacgattt cggcaatgtg ttcttcggtt
    17401 aagacgttgt tgttggtttc ttttttaaag aagccgcctg cgtcgatgaa ttggatgtcg
    17461 gtattgtctt tgtgtttgga caaaaccagt atattgacgg cgatgccggt gccgtaaaag
    17521 agattgggcg caagggcgat gacggtttcc acgtagttgc cctccaccag atattggcgg
    17581 attttctgct ctgcgccgcc gcgatagaaa atgccgggga atgagacgat ggcggcgcgg
    17641 cctctgccgg aaaggtagtt cagtgcgtgc agaatgaagg caaaatcggc tttggatttc
    17701 ggggcgagta cgcctgcagg ggcaaagcgg tcgtcgttga ttaaggtggg gtcgccgctg
    17761 cctatccagt tgatggaata gggcggattg gaaacgacgg catcaaaggg tttgctgtct
    17821 ttgagtttgg gattggtcag tgtgtcgccc aattcgatgt ggaatttgtt gtaattgacg
    17881 ttgtgcagaa acatattcat gcgggcgagg ttgtaggtgg tgtggttgat ttcctgcccg
    17941 aagaagcctt cttcgatgat gtgctcgtca aactgttttt tcgcctgcaa gagcaggctg
    18001 cccgagccgc aagctgggtc gtagattttg tttactttct cctgcccgtg caccgccagc
    18061 cgcgcaatca gcttggatac ggatacgctt tgcggggtga aaaattcgcc gccggatttg
    18121 cctgcgttgg cagcgtagtt ggaaatcagg tattcgtagg catcgccgaa aaggtcgatg
    18181 tggtggtctt caaaactgcc gaaatcgagt tccgccacgc cttttaggac ggcggcaagg
    18241 cgtttgttct tgtcggcaac ggtgctgccg agccggctgc tggtggtgtc gaagtcgtca
    18301 aacaggcctt taatgtcttg ttcggacgga tagccggagg cggagctttc aatcgcggta
    18361 aaaatttctt tcagcttggt gttgagctct tcgttttgat gggcttcggc ggcaatattg
    18421 caaaaaagct ggccggggta gatgaaatag cctttgactt tgacggcatc gtctttgatt
    18481 tcgggcgtga tgatgctgtc cggcatagcg gcgtaatcaa tactgctgtc gcctgcctgc
    18541 atatagtcgg taaagttttc gctgataaag cggtagaaaa gtgtgccgag aacgtattgt
    18601 ttaaagtccc agccgtccac cgcgccgcgt acttcgtcgg caattttcca aatttggcgg
    18661 tgcagttggg cgcgttgttg catttcggtc atcatcgaaa tccatataaa agttaaacaa
    18721 atcgaaatcg cctgatattt tcagacgatt ttttacgggc attcaaatta agccaaattt
    18781 tccagcatcc atttgacgta gcggtcgacg cctgatttga catcgaaaaa ttcttcctta
    18841 tatccggctt cgcgcaattt ggtgatgtcg gcttgggtga agctttggta tttgcctttg
    18901 agcgcgtcgg gaaatgggat gtagcggata aattcttctt ctaccaactc ttttaaactc
    18961 atttcaggtt tgccttcggc ggcgcggcag gcgttgacgg tggcagcggc gagttcgtta
    19021 aactgttggc tgcgacctgt tcctaggttg tagatgccgg aaagttcggg atggtcgaag
    19081 aagtagaggt tgactttggc aacgtcttcg acgctgacga agtcgcgggt ttgttcgccg
    19141 ttgccgtagc cgtcgttact gccgaacagg ttgacgtaac cgtgttcgcg gtattggtgg
    19201 aagtggtgga aggcgacgga tgccatgcgg cctttgtgtt gttcgtgttg tccgtaaaca
    19261 ttgaagtagc ggaagccgac gacttgggcg gtgagacctt ctttcatgcg gcgacgcaat
    19321 acttggtcga acaggaattt ggagtagccg tacacgttga gcggtttttc gagttcgcgc
    19381 tcttcgcgga agatttctcc tttgccgtaa accgccgcac tggaggcata aaggaagggg
    19441 atgcgttcgt cctgacacca gtccagcaaa tccagcgtgt actggtagtt gttttccatc
    19501 atatacaaac catcgtggtt catcgtatcg gaacacgcgc cttgatggaa aacggcttcg
    19561 atgttttgat aaggtaaaat gtgttccctc acttggcgga tgaattcgtg tttgtcgaga
    19621 taatgggcga tttcgcactc ggcaaggttt ttgaattttt cgcctttgct caaattatcg
    19681 acggcaacaa tgtcagtaat accgcgttga ttaagtgctt tgacgatgtt gctgccgata
    19741 aagccggccg cgcctgttac gatgatggtc atattcggtt tcctttgttt tgagtaaaat
    19801 taataaactt agttacaaga aatcatcgat attgtcctga aacacaaatt tgcgatgata
    19861 ttccaaatat ttttctcttc cttcgggata tattctcatc ttaatttttt gcatttggat
    19921 tgatattaag tttttcccat gtgtaacggc ttagtcgagt accgcaaatc aaacgaccat
    19981 catccaagaa agtaatataa ccttgatcga acaaccaatc ataagtcggc gttaaagcta
    20041 acccatttaa ataatctaat gcctctttct catttttctc agtaatacaa accatataag
    20101 gtttaatatg actagccctt aacaaaatct catctgtaat taatgtaaat ggacattgcg
    20161 acatatgatt tataacatcc ttgcgatatt tctgctgccc ttttctactt ttctgtttaa
    20221 tgatttcttg ctctacgaat tgattaaatt ctgaagccgg tatttctaat ttttctcttg
    20281 ataagagttg cgggtgcacg atagagcgaa attgataatc caaaaatatt cggaaataaa
    20341 ataatggttc tgaatcttct atatctttga caggcaatag tttaagaatt gataaataac
    20401 taattttggg caatacaatc ttacgccaaa ttgaccatat atcatcctcc gaacggatat
    20461 aacccctacg gtaaccttta gaattttcca aatggtctga tacatcatgg attgaaaaga
    20521 aaatattttc tgtaggcaga ttattaatac ttaaaacgta atcatcataa aaccttgaaa
    20581 tatcatgatg atatttctgt tcttgtttgt gatattcaac ttttgcatca accaaataac
    20641 gctgtaaatt tttcttatta aaaaaacaat ttttctgaat gaaaggatat tctaccctcc
    20701 ttccattttt tgtctcagta atcccccaat tttcatactc aaaaaatgta cttagttttc
    20761 gaccactatc atcaatataa ctacctacat atttgcgtgc ttccccatta ccaccaaaaa
    20821 cagataactt gttattcctg tgaataaaac tatcttccgc acgtaaattt tgaatactat
    20881 cgacaataaa atactctatg ttttcaatag taattctttg aaaatccggc attttactct
    20941 ccataaagcg taatatccat ttgttttaat atagcgatta atacatcaac aacaatactg
    21001 tttcccgctt ggcgatacat ttgagtatca ctaactaaaa ttttaaaatc atctctaaaa
    21061 cccattagcc gcaaacattc cctaggtgtt aatttacgaa tacgtccacg attatgagta
    21121 acataattat caaccccggc acgatgcatt ttatgcatag tttgcaataa tgggcgagct
    21181 acaggcaaat cagtttccgt actggtttta aaattttttg ttcctcctgc caaaacataa
    21241 tttttgattt tttcagaaag ataatatttt tcctcaacgt tatttacatc aaaaataaag
    21301 tcatcaaatt cagattcgcg ggctgcctga aaaataaaat caccatgcca attaaattgt
    21361 tgatttgctt tttggcataa ggcaatttct ccattaatct gtgtataacg tttttgtcta
    21421 tttttagaac tggtaacaaa tttcacaccc ttttcacgta aaaaatattt gctatcagta
    21481 tagtcctcca aaaaatcttg catagtatgt tctaattcaa tcttttcagg aaactgaaaa
    21541 ccatttatag gaggggtatg aaagccaaca acaaaaatac gctcacgatg ttgaggaatc
    21601 ccataatcct tactattcat tatttggaaa tataagtcat aaccaagtga ataaaaaaca
    21661 ctttttacaa ctttccaagt ttttccatta tcatgattga gcaagccctt tacattttca
    21721 taaataaata tctttggttg gacttcatcc acaacgcgag caaattcata gaataatgtt
    21781 cctcgtgtat cttctaatcc tgcacgtttg ccaaccatgg aaaatgcttg gcaaggactg
    21841 cctccaacta aaatatcaac ttgatttcta aactttctcg catcaaattg agtaatgtcg
    21901 ttatgccaaa aatcttcatt taatttatag tttccaagat aacttttttt aacgtatgga
    21961 tcaatatctc ctgcaaaaac aatggtatgg tttaaattta atcggtggaa tgcctgttca
    22021 accgcaccaa tcccgctgaa tacggttgct aatctaatat gtgaatcagg tttaagaaaa
    22081 gttttagatt tccaaccttg ttgactggga aagagcaaag ttttttgtaa tcgagtatcg
    22141 tgtgtctgtg ctattgtcga aatagtcata cttatatcgt tctgtttatc ttatcaatat
    22201 gaaaactaca ttgttgattg ccctgacaat gccttgatca attcggcaaa cgagcaaacc
    22261 gccgtaccga gtttcgccac gacaaccccg gccgcagtat tggcaaggta catggcttcg
    22321 ggcatggtgc agcctgccgc caaacccaag cccattccgg caatgacggt gtcgcccgca
    22381 ccggatacgt cgtaaacttc ttgggcgcgg gtgggctggt aaatcggttc gccttcgctg
    22441 aacaaggtca tgccttcttc gcttcgggtc agtaaaacgg cggtcaggtc gaggtggcgg
    22501 cgcaggtttt gcgctttttc ggtcagctcg ctttcgtttt tccaactgcc gaccacttct
    22561 ttcaattcgg cgcggttagg cgtaatcaga gttgcaccga catatttttc gtaatcgtcg
    22621 cctttggggt cgattaagac ggttttgccg gcgtgtttcg cccaatcgat catatcggag
    22681 atatgcgaca ggccgccttt gccgtagtct gaaaaaatga ttgcgtcgta ttcgggcaag
    22741 atttcgcggt atttctgctt gatttgttcc aacacttcgc agttgggatg ttcttcaaaa
    22801 tcaagacgga taagctgctg gttgcgggcg acgacgcgca gtttgacggt ggtggcgatt
    22861 tgtttgtcgc gcatcagata ggaggcgacg ccgtcctgca ccatcagcgc atcgagcgcg
    22921 tcggcggctt cgtcgttgcc ggttacggac aacagccctg ccctgccgcc caacgaagcg
    22981 atgttgcgcg cgacatttgc cgctccgccc gcgcgttggt cgattcgtcc gattttcgcc
    23041 accggcacgg gggcttcggg cgaaatacgg gacacatcgc cgaaccaata gcggtcgagc
    23101 atcacgtcgc cgacaaccag gactttggct tgcgcgaaac gggatttgag ggtttcttgt
    23161 tggaacttgg cggacatatt gcctccgttg tattttttca gacggcctca gcctgtaagg
    23221 ccgtctgaaa atcagtttgc ttcaaggttt gccacgcggt tcacttcgcg caatacggct
    23281 accggatcgg cagcttgggt tacaggacga cccattacca aataagtcga accggcagcc
    23341 aaggcttcgg ccggtgtcat gatgcggcgc tggtcatcat tattgtcggc aacgtccaag
    23401 cggatgccgg gcgtgaccaa gacaaaatcc tgtcccaatt cgcggcgcag cggcgcggct
    23461 tcttgggcgg aacagaccac gccgtccaag cccgaacttt gcgccagttt cgccaagcgg
    23521 atgacttgtt cttcaggggc ggtgttcaaa ccgatttccg ccaaatcgct ttgttccatg
    23581 ctggtcaaca cggttacgcc gattaagagc ggcttcgtgc cgtatccggc aacggcttct
    23641 gctgcggctt ccatcatacg gcggccgccc gatgcgtgca tatcgaccat ccaaacgccc
    23701 atatcggcag cgactttgca ggcttgcgcg acggtgtggg gaatatcgtg gtatttcaaa
    23761 tcgaggaaaa gtttgaaacc ttgattgatt aagctttctg ccaaattgcg tcccgtcgcg
    23821 gtaaacagct ctttgccgat tttgatttga cacaatgtcg ggtcaaggtt gcggacgaat
    23881 ccgagcgtgt ctttttcgtt ggcaaaatca agggcgacga taacgggggt gcgttgttgc
    23941 ggagtttgga agtcggagat taaaggattc atagtgtttt cgtttcgggt tttcagacga
    24001 cctttatcgt ctttttcgca gtatagtgga ttaactttaa accggtacgg cgttgtctcg
    24061 ccttagttca aagagaacgc ttctctaagg tgctgaagca caaagtgaat cggttccgta
    24121 ctatctgtac tgcctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaca
    24181 acgtaaattc aaaccgtagg tcgggcattg atgtccgacc tacgcggact caagcctccg
    24241 acaaactgtt tcggataaat ttgaaccggt acatcagccg tcattcccgc gcaggcggga
    24301 atccattgat aaaactcagt tcatcagatt taaacagcga ttgcccaaat tcagaaatgg
    24361 attcccgcct gcgcgggaat gacgccaaat gattattttt cggttgcaac acgctgttta
    24421 tcaaaaacag gtcgtctgaa aacctgattt tctgttttca gagtgcgaag cctgcccgtg
    24481 ggtacgacct tttcgattta tttgcccaat gcgcccaata cttcggcttt cacggcttct
    24541 accggctgag tgccgtcaac tttgatgtat ttaggcgcgt gttcgccttc cagtttgctg
    24601 taaaaatcga ccaaaacttc ggtttgctcg tggtaaacgg caaggcgttt tttcacggtt
    24661 tcttctttgt cgtcgtcgcg ctgaatcaaa tcttcgccgg ttacgtcgtc tttgccttcg
    24721 actttgggcg ggttgtaggt aacgtggtaa gtacggccgg aagccaaatg cacgcggcgg
    24781 ccgctcatac ggtcgacaat cacgctgtca ggcacgtcga tttcaacgac tgcatccaaa
    24841 tccacgcctg cttcaaccat cgcttcggct tgtgccaatg tgcgcgggaa accgtcgaac
    24901 aggaaaccgt ttttgcagtc gtcttgcgcg atgcgttctt tgaccatgcc gataatgatg
    24961 tcgtcgcgca ccaagccgcc ttcgtcaatg atttttttcg cttccaaacc caagggcgtg
    25021 ccagccttaa tcgcggcgcg gagcatgtcg ccggtagaga tttgcggaat gccgaacgcg
    25081 gcggtgatga attgcgcctg agtgcctttg cccgcgcccg gcgcgcctaa aagtaatgct
    25141 ttcatgaagt gatcctttga aatgtgttta ttggaatcaa acagatggtt tacgattcta
    25201 cacggcttgg cacaatagtt caaaaaccaa ctgcacaaac tgccatttgc aagcaaatgt
    25261 tttgcatacc tttgccgagt ctgatattca caagccggca actttttggg acaatcccca
    25321 aaatttcaaa acatcacacc ctaccaaaaa ggaacatccg tgaaacgaat ttttctgttt
    25381 ttggctacca atatcgctgt tttggtcgta atcaacattg ttttggcggt tttgggcatc
    25441 aacagccggg gcggcacggg cagcctgttg gcgtattccg ccgtcgtcgg cttcactggt
    25501 tcgattattt cgctgctgat gtccaaattc atcgccaaac aatcggtcgg cgcggaagtt
    25561 atcgacacgc cgcgcaccga agaagaagcc tggcttttga acactgtcga agcccaagcg
    25621 cggcaatgga acctgaaaac gcccgaggtc gccatctacc actcccccga acccaatgcc
    25681 tttgccacgg gcgcatcgag aaacagctcc ctgatcgccg tcagcaccgg tttgctcgac
    25741 catatgacgc gcgacgaagt ggaagccgta ttggcgcacg aaatggcaca cgtcggcaac
    25801 ggcgatatgg ttacgctgac gctgattcaa ggcgtggtca atacctttgt cgtgttcctg
    25861 tcgcgcatta ttgccaacct gattgcccga aacaacgacg gcagccagtc gcagggaact
    25921 tatttcctag tcagcatggt attccaaatc ctgttcggct tccttgccag cttaattgtc
    25981 atgtggttca gccgacaacg cgaataccgc gccgacgcgg gcgcggcaaa actggtcggc
    26041 gcgccgaaaa tgatttccgc cctgcaaagg ctcaaaggca acccggtcga tttgcccgaa
    26101 gaaatgaacg caatgggcat cgccggagat acgcgcgact ccctgctcag cacccaccct
    26161 tcgctggaca accgaatcgc ccgcctcaaa tcgctctgag ccgatttgaa acgacaaaaa
    26221 ccgtacgcgc aagcagtacg gttttttcat agtaaaccaa tgccgtctga aaacatcggg
    26281 tttgccatac cggcaccaac cgtatccatt tccgcaccgg atacgccgca ttgatatacc
    26341 ctgataaacc gtatccggac agaaaatata gtggattaac aaaaaccagt acggcgttgc
    26401 ctcgccttag ctcaaagaaa acgattctct aaggtgctga agcaccaagt gaatcggttc
    26461 cgtactattt atactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccatata
    26521 tcagataaaa cggcagcaaa aatgccgtct gaaaccaatt agggcttcag acggcatttt
    26581 tactgcaaaa ccttatgcga acggatgatg cagcacaatc gtttcttcgc ggtcggggcc
    26641 ggtggagacg atggcgaccg gcgcaccgca gacttcttca atccgtttca aatatgcttt
    26701 ggcattggca ggtaatgcgt cgtagctttt cacgccgaaa gtggattcgc tccaaccggg
    26761 catggtttcg taaatcggct tgcaggcttc taccgcatca gagccgcaag gcaggatgtc
    26821 ggttttgccg ccgtcgggca attcgtagcc gacgcagata ttgatggttt caacgccgtc
    26881 cattacatcg agtttggtaa tacacatacc ggaaatgccg ttgatttgga tggagcgttt
    26941 cagggcggcg gcatcaaacc agccgcagcg gcgtgcgcgt ccggttaccg aaccgaattc
    27001 gtgtccgcgt tctgccaaac ctgcgccgac ttcgtcgaac aattcggtcg ggaacgggcc
    27061 cgaaccgacg cgcgtggtat aggctttgac gatgcccaaa acataatcca gcatttgagg
    27121 acctacgccc gcgcctgcgg aagcggctcc cgccagacag ttggacgagg taacgaaggg
    27181 ataagtgccg tagtcgatgt ccaacaacgc accttgcgcg ccttcaaaca gcagtttttc
    27241 gccgtttttg tttttctcgt tcaacacgcg ggacacgtcg gtaatcatcg gcgcaatgcg
    27301 cggcgcgact ttttcgataa ccgccatcac gtcttccgct ttaaccggtt cggcattgtg
    27361 cagatgttgc agttggacgt tgtaataagc aaggacgaca tccagttttt cacgcagttt
    27421 ttcaggatgc agcaaatcgg cgacgcgaat ggcgcggcgt gccactttgt cttcgtaggc
    27481 aggaccgatg ccgcggccgg tcgtgccgat tttgcctttg ccgcgcgatg cttcgcgggc
    27541 ttggtcgagc gcgatgtgat aaggcaggat cagagggcag gtcggcgcga ttttcagacg
    27601 gccttcgacg tttttcacgc ctgccgcgtt caactcgtcg atttcgccca acagggcttc
    27661 gggggagacg acaacgcccg aaccgatgaa gcagtccaaa ctttcatgca ggatgccgct
    27721 cggaatcagg cgcaaaatgg tttttttgcc gccgacaacc aaggtatggc ccgcattgtg
    27781 gccgccttgg aagcgcacca cgccgccggc ttcttccgcc agccagtcaa cgattttacc
    27841 tttaccctcg tcgccccact gtgcgccgat tactacaaca tttttagcca tagccatata
    27901 acctatcgat attaaaaatt attgcggaaa tccccaaacc ggtgcggctt tgcgccccgc
    27961 cacgatttgc ctttcatccg ccatgaacgg gtatttagcg tttcacgacc tgccaaacgc
    28021 cgtccgtctt tttcagacgg cctgcaagct cttccgaaac attgtgtccg ataccgtaat
    28081 cgattacgac acactgccct tgttcacgca aggcttcgac cgcttcgtgc gccgcttcgg
    28141 catcttccgc atcgaccaac acggcgggct gccgttcgat ggcgggcaaa cgcccgataa
    28201 agctgcgcaa gtcgaaactg aatcccgttg ccgggcgcgc cctgccgaaa tatccgccca
    28261 atccgtcata acgcccgccg cgcgcgaccg cgtcgtggaa atcggcggca taggcggcat
    28321 acagcaagcc cgtgtggtaa ttgtcgacac gcagctcgga caagtcgata tggatttcac
    28381 aatcggggaa tgcgtcgcac accgcctgca attcgtctaa cgcgccgccg accgccgaca
    28441 aatccggcaa ccgtccgcgc gcgtcggaca acacttcacg cccgccgtac aggcgcggca
    28501 acagcgagaa tgcttttgcc cacatgccgt ccagcttcca agccttgacc tgcgcttcga
    28561 ccgccccggt atctttatcc tgcatcaagg caagcagcgt tgcggactgc cccgcatcca
    28621 aatgtgccgc atcggacaag gcgcgaaata tgccgatatg ccccagcgaa agcagcactt
    28681 tgcccatatc ggcaattttc atgcttttca gcataaggtc tatcagctcg atgtcgccac
    28741 ggatatcggc aaaaccgtac atttccgccc ctgcctgcaa gggttcgcgc atattcagca
    28801 gaccgtcgga ctgcgcgtgc aacaccggac cggcataaca caaccggtta atcccttggt
    28861 tggcggataa aagatgggca tcgatacgcg ccacctgcgg cgtgatgtcg gcgcgtatgc
    28921 ccaactgcct gccgctgagc ctgtccgtta ccaaaatggt tttcagggaa agccccgcat
    28981 cgatatgcgt cagcagggaa tgtgcgtact ccatcagcgg aggctgtacc agttcataac
    29041 cgtgtacgcg gaacagtgcc aacaactgct ccctcgcgct ttcaagctgc cgcgcgttcg
    29101 tgggcagtac gtcggcgata tgttccggaa gctgccatgt ctgcatgaga atctgccttc
    29161 tattttatcc tgtaaacaca aaagactgcc cgatccgcaa tcagacggca tctttgccgc
    29221 aacgcatcac gccgctgtca aaacggcgat gcttctttct gattcagaat ccgaacagcc
    29281 cctgtccgac ggctcaatgc cgtcaagtcg ttaaaaccaa actttaccat aaaatacaca
    29341 caatctgaat atacttttat accctgtatc cgccgtctga cggcacggca tttttttact
    29401 atataatgtg caccattatt tctcatcact tcctgctgcc gttttctgct tcagacggca
    29461 tttttgttga ttgaacactt atgctgctcg acctcaaccg cttttccttt cccgtcttcc
    29521 tgaaagaaat ccgcctgctg accgcccttg ccctgcccat gttgttggca caggttgcgc
    29581 aggtgggcat cggttttgtc gataccgtga tggcgggcgg tgcgggtaag gaagatttgg
    29641 cggcggtggc tttgggcagc agcgcgtttg ccacggttta tattaccttt atgggcatta
    29701 tggcggcgtt gaacccgatg attgcccagc tttacggcgc gggtaaaacc ggtgaagtgg
    29761 gcgaaacggg gcggcagggg atttggttcg ggctgatctt ggggattttc ggcatggtct
    29821 tgatgtgggc gacgattacg ccgttccgca actggctgac cttgagcgat tatgtggaag
    29881 gcacaatggc gcagtatatg ctgttcacca gcttggcgat gccggcggca atggtacacc
    29941 gcgcactgca cgcctacgcc tccagcctga accgcccgcg cctgattatg ttggtcagct
    30001 ttgcggcgtt tgtgttgaac gtgccgctga actatatttt cgtttacggc aaattcggta
    30061 tgcccgcttt gggcggcgca ggctgcggac tggcgacgat ggcggtgttt tggttcagcg
    30121 cgctggcatt gtggatttat atcgccaagg aaaatttctt ccgcccattc ggactgacgg
    30181 cgaaattcgg caaaccggat tgggcggtgt tcaaacagat ttggaaaatc ggcgcaccca
    30241 tcgggctgtc ttattttttg gaagccagcg cgttttcgtt tatcgtgttt ttgattgcgc
    30301 ctttcggcga ggattatgtg gcggcgcagc aggtcggcat cagtttgtcg gggattctct
    30361 atatgattcc gcaaagcgtc ggctcggcgg ggacggtgcg catcggcttt tcgcttgggc
    30421 ggcgcgaatt ttcgcgggcg cgttatattt cgggcgtgtc actggtgtta ggatggatgc
    30481 tcgccgtgat taccgtgctt tccttggtat tattccgttc gccgctggta agtatgtaca
    30541 acaatgatcc ggcggtttta agcatcgccg ccaccgtctt actgttcgcc ggcttgttcc
    30601 aaccggcaga cttcacccaa tgtatcgcct cctacgcctt gcgcggctac aaagttacaa
    30661 aggtgccgat gttcatccac gccgccgcct tttggggctg cggtctgctg ccgggctacc
    30721 tgctcgccta ccgtttcgat atgggcattt acggcttctg gacggcattg attgcctcgc
    30781 tcaccatcgc cgccatcgcc ttggtgtggt gcttggaatt gtgcagtagg gagatggtca
    30841 gatcgcataa ggccgtctga aaactccgcc gattaaaaat atagtggatt aacaaaaacc
    30901 agtacggcgt tacctcgcct tgccgtacta tttgtactgt ctgcggcttc gttgccttgt
    30961 cctgattttt gttaatccac tatagaaacg atgggctgaa gcccgccact ggcaatactc
    31021 attttaaagt tcaaacccat gcaacccatc cgataccgaa ccgaccttac cccctacaac
    31081 actttcggcc tgcgcgccca agcccgagcc tttattgcgc ttgaacacgc cgacgggttg
    31141 cgcgacatcg tccggctacc cgagttcaac cgagatactg ttttatggct gggcggcggc
    31201 agcaatatcc ttttgatgga ggactacgcc ggtctggtcg tacatatgga aaacaaaggc
    31261 atacgcgaaa ttgctcgttc agacggcatg gttctgattg aagcgcaggc gggcgaaatc
    31321 tggcacgatt ttgtcctgca cgccgtcgcg ctgggtttga acggtttgga aaacctgagc
    31381 ctgattccgg gtacggtcgg cgcgtcgccc gtgcagaaca tcggcgcata cggcgtagag
    31441 gcgaaagatg tgattcacag cgtgcgctgc tttgatttgg atacggaggc ctttgtcgag
    31501 cttgccaatg ccgactgccg cttcgcctac cgcgaaagcc tgttcaagca ggaaggtaaa
    31561 gggcgttatg tgattgtttc ggttgtgttt gcattaaaag agcattttgt gccgaacttg
    31621 ggctacggcg atttggcggc agcggttgcc gaacggtgcg ccggcaggga aataacggcg
    31681 aaagaagttt ccgatgcagt gtgcgccgtc cgccgccgca aactgcccga cccgggcatt
    31741 ttgggcaatg tcggcagttt ctttaaaaac cccgtcgtcg gcgcgggaaa agccgccgcc
    31801 ctgttgcagc agcatcccga tatgccgcat tatccacagc cggacggttc ggtcaaactt
    31861 gctgccggct ggctgatcga ccaatgccgt ctgaaaggct tccaaatcgg cggcgcggcg
    31921 gtacacgaca aacaggcatt ggttttggta aacaaaaaca acgcctcggc aaacgatgtc
    31981 cggcaattgg cgcaacacat caaatttaca gtatttgctc agtttcaggt agaattgcac
    32041 gccgaaccta attggctgcc tgcttcgttc agcctgtaaa tccaaggagt atgcccgtga
    32101 cccgccccgc caaaatcaat acttacacac gcatcatcga cgccagcctt gcgctcttca
    32161 acgaggaagg cgagcgcaac atcagcacca accatattgc cgcccacttg ggcatcagtc
    32221 cgggcaacct ctattaccac ttccgcaaca aagacgaaat catcgtccaa ctgttcaaac
    32281 gttacagcga agccctgctt gcctacctga atgaagctgt gttgccgtct gatgtggaag
    32341 actccatcaa ctatatggcg ggcatttacg acgtgatgtg ggaataccgc ttcctattca
    32401 gcgacgtgaa caccctgctt gcacgcagtg ccgaactatt aggcgaacac aatactttca
    32461 cccaagccaa agtctccccg ctcttggtca acctgctgac ccagctcaac ggtctgaaca
    32521 tcatccaagc cgaccaaacc gctatgaacg atctcgccgt caatatgtgg atggttacga
    32581 aatactggtt cgacttcgac agctccctgc gcggccgcac caagctgacc gaagactcca
    32641 aagcacgcgg catccgccgc accttaagcc tccttcgtcc ttatcttttg cccgaacacc
    32701 gcaagaaata cgaccggaaa atcggcaacg gcaacccgta aacccacagc gtccgaatca
    32761 gccttcagac ggcacttacc cgacggtatc agcgaatgcg cgcagtcccc ccgccccata
    32821 tctccatcac aggcctaggt tacctcggcc tgccgctggc acaaaagttt taccaacacg
    32881 gcagccgcgt cgccgccata aaacgcagcc tgacttcgga cgacatcaac ctgcccatac
    32941 acctcgatac catcgacctc aatcaagaca gcgcgtttca aagcgcgaac cttgcccgag
    33001 atacaagctt ttggcggcac catgccaaca aacccgtttg gttctgcctc ttgccgccat
    33061 catcgctgac acattacgcc gataccgtca aacaatgggc agaacttgcc cgggcgtgca
    33121 acgtgcaaca cctgattttc acaagcagta ccagcgttta cggcgataca gcgcgcgaat
    33181 gcgacgaaat cgccctaccc gatccgcaaa ccgagtccgc ccgccaaatc ctcgccgccg
    33241 aacaacacct gctcgacagc ggcgttccga acatcgacat cctgcggctg ggcgggcttt
    33301 attgcgccga acgccatccc gtcagccgcc ttgttcaaaa gcaaaacatc ccgggcggca
    33361 accgccccat caacattgtc caccgtaata tcgccgtcga aagcctgttt cagacggcat
    33421 tcaaacccgg cggcaggcgg ctgaaaaaca ttatcgaacc gcgccacccg acacgacgcg
    33481 aattctacac ggaagaagcc gccaaactcg gtttgccggc tcctgatttc gcacccgacg
    33541 acagcatggg caaaatcatc cgtaccgttt gcgataacag ctgaagcctg taaaataagc
    33601 gtcaacagca acaaacaggc attctccgcc ccgatcagaa aatatgtccg ccctcctccc
    33661 catcatcaac cgcctgattc tgcaaagccc ggacagccgc tcggaacttg ccacctttgc
    33721 aggcaaaaca ctgaccctga acattgccgg gctgaaactg gcgggacgca tcacggaaga
    33781 cggtttgctc tcggcgggaa acggctttgc agacaccgaa atcaccttcc gcaacagcgc
    33841 ggtacagaaa atcctccaag gcggcgaacc cggggcgggc gacatcgggc tcgaaggcga
    33901 cctcatcctc ggcatcgcgg tactgtccct gctcggcagc ctgcgttccc gcgcatcgga
    33961 cgaattggca cggattttcg gcacgcaggc agacatcggc agccgtgccg ccgacatcgg
    34021 acacggcatc aaacaaatcg gcaggaacat cgccgaacaa atcggcggat tttcccgcga
    34081 atccgagtcc gcaaacatcg gcaacgaagc ccttgccgac tgcctcgacg aaataagcag
    34141 actgcgcgac ggcgtggaac gcctcaacga acgcctcgac cggctcgaac gcgacatttg
    34201 gatagactaa ccttcagacg gcatccgaca tgaacagccc ttttcacaac atcggcatcg
    34261 taacccgccc caacacgccc gacatccaag acaccgcaca cacgctgatt acctttttga
    34321 agcagcacgg ctttaccgtc tatctcgacg aagtcggcat aaaggaaggc tgcatctata
    34381 cccaagacac cgtcggctgc catatcgtca acaagaccga actggggcaa tactgcgacc
    34441 tggtcgccgt tttaggcgga gacggcacct ttctctccgt cgcccgcgaa atcgccccgc
    34501 gcgccgttcc gattatcggc atcaaccaag ggcatttggg cttcctgacc caaattcccc
    34561 gcgaatatat gacggacaag ctattgcccg ttttagaagg gaaatacctt gccgaagagc
    34621 gcatcctgat tgaggccgca ctcatccgcg aaggcaaaac cgccgaacgc gccatcgccc
    34681 tcaacgatgc cgtcctctcc cgtggcggtg ccggacagat gattgagttt gaagtcttcg
    34741 tcaatcggga attcgtctat acccagcgtt cggacgggct gattgtctcc acccccaccg
    34801 gatcgaccgc ctattcgctt gccgccggcg gccccatcat gcaggcaggt ttgcacgcct
    34861 tcacactcgt acccatctgc ccgcaatcca tgaccaaccg ccccatcgcc attcccgaca
    34921 cgtccgaaat cgaaatcctc gttacccaag gcggcgacgc gcgcgtccat ttcgacggtc
    34981 aaacccatat cgacgtgcaa aacctcgacc gcatcaccat ccgccgctac cgaaacccct
    35041 tacgcatcct acatccgacc gactaccaat atttcaaaac cctgcgccaa aaactgcact
    35101 ggggcgaaca attagtctaa gccggccttt acccgaaagt acccatgaat tccaaaattt
    35161 cctccgacca tacagaagat accatgcgcc tgtccaaacg tatggcgcaa ctggggcttt
    35221 gttcgcgccg cgaagccgac ggctacatcg aacagggctg ggtaacggtc aacggcaaaa
    35281 ccgccgtact cggtcagaaa gtttcgccgg cagaccatat cgaactaaac aagaaagccc
    35341 acgaacagca ggcggcacgc attaccatcc tattgaacaa acccgtcggc tatgtcagcg
    35401 cacaagcgga aaaaggctat aaatccgccg ccgaactgat tacccctgaa aatcgctggg
    35461 aaggcgacac cggccgcatc cgtttcgatc cgaaacacaa aatcggtctt gcccccgccg
    35521 gcaggctcga tatcgattca gtcggattac tggtgttgac gcaggacggc cgtatcgcca
    35581 agcagcttat cggcgaaaac agcggcagtg aaaaagaata tttggtgcgc gtgcgcggca
    35641 aattggacga aaaaggactt gccttactga atcacggatt gagtttggac ggcgagaaac
    35701 tgcgtcccgc ccaagtagaa tggcaaaacg aagaccaact gcgcttcgtg ttgaaacagg
    35761 gtaaaaagcg gcaaatccgc cgtatgtgcg aactggtcgg actgcgcgtc gtcgggctga
    35821 aacgcatccg catgggcaag gtcaaactcg gcaggctgcc gcccggcaaa tggcgttatc
    35881 tcgcccccgg cgaatcgttt taaataagca ttaaaaaatg ccgtctgaaa atccttgcgt
    35941 tttcagacgg cattgcctta tgcccaaata acggtttctt ccaagggcct gcgcgctttc
    36001 gtggcgattt cctgaacgcg gtaaccgaat gtcgcggcga cggacacgcc ccattctgcc
    36061 gcatcgaaca aaccgaactg cccggacaat acgcgttcca tatcggcata gttgaaacct
    36121 tccaccgggc aggaatcgat acctgccatc gccgcacccg tcatcatgtt gcccaacgcg
    36181 atataggtct ggcggcaaca ccagtcaaac aaggcgcgcg aatcgtccaa aatcttgatg
    36241 tcgtcggctt gaaacgcccg atacctcgcc aaagattttg ccatggcatc cggttcggta
    36301 acgccgcgcc gtttgaggct ttccaacata aacgggctgt cgaagcgggc atttttcttt
    36361 gccaaaaaca ccaccaaatg actggcggta tccaaagcat ccgccatacc ccaagaaaac
    36421 ggcttgattg cctgtcggat ttcggggttt tgaaccacaa caaactgcca aggctccgaa
    36481 ccgaccgaac tgggcgacaa acgcccgagt tctaaaataa actgaaaatc ctcggcactg
    36541 attttacgtg ccgcatcgta atgccggcat gatttgcggt ttttaaatgc ggatagaacc
    36601 tgctctctgt ctaataccgt catagtgcat cctttcatta cttgtattgc aaattgattt
    36661 caaaccgata cggcggcaga ttcgccaata acgcccaaac gcctttcaga cggcatttgg
    36721 ttcatcgcaa acacgcctac gggtaggcga tataggcata agccgaatgg ttgtggatag
    36781 actcgaaatt ctcgctctcg acgacgaaac tcttgatgcg tttgtcggcg atcagcgaag
    36841 tagcgacatc gcgcaccata tcttccacga atttcgggtt ttcgtaggcc ttttcggtaa
    36901 cgtatttttc atcggggcgt ttgagcaggc cgtagagttg gcagctcgcc tgcgcctcca
    36961 cataatcgat gacttcctcg ataccgactt cggcatcggc agtcaggctg acggtaacgt
    37021 gcgaacgctg gttgtgcgcg ccgtattggg aaatttcttt ggaacacggg caaagcgagg
    37081 ttacgggaat catgaccttc atactgtggc cgtatgcacc gtctttcatt tcgcccgtga
    37141 ggcagacatc ataatccagt aaagactgaa taccggaaac cggggcggtt ttcttgcgga
    37201 aaaatgggaa agaaacgctg attttaccgg cgcgggaatc caaaagcgcg accatttcgg
    37261 cagtcagcct atgcaattgt gcgaaatcca aggcttcaga atattgctcc atcaatgcga
    37321 caaagcgcga catatgcgta cccttctgct cggcgggcag gtaaaccgtc atggtcaggc
    37381 gggcaaccgt ggattgcgtt ccttcggcgg ttttcagggt aatcgggaag cgcaggtctt
    37441 taatgccgac ctgattgatc ggcaggttgc gtaaatctct gctggattgc acgtctgcaa
    37501 tggcgttcat gcgttgtttg tccttaatat tcagataatt attgagatgt gtgtattgta
    37561 tggcaggcag atgccgtctg aaaaaacgct gttggccgcc tgccttgcaa aggcgaaatt
    37621 atatcacttg cttttggcgg ctgcattgat tgtttctatt ttcgggatag tgataaaatc
    37681 ccgacttatt catatcttac aaggcagaaa cagatgaaat tcgccaccca aaccatccat
    37741 tccggctacg attgcgacga acacaaccgc gcgctgatgc cgccgattta tcaaaacagt
    37801 atgtttgcgc tgcacgaaat cggcgaaaat gtgccttacc gttattcgcg cctgagcaac
    37861 ccgacccgtc aggtattgga agataccgtt gccgatttgg aacacggtgc ggccggcttt
    37921 gcgttttcca gcggtatggc ggggattgat gccgtgtggc gtactttcct gcgcccgggc
    37981 gatactattg ttgccgtcgc cgatatttac ggcggcgcgt atgatttatt ggtcgatgtt
    38041 tatcaaaaat ggggggtgaa cgttgttttt gccgatttgg gcaatccgga taatctggac
    38101 gaactgctca aagcgcacaa tgtcaaattg gtttggctgg aaacgccgtc caatccgctt
    38161 ttacgcttgg tagacatcaa agcgcttgcc gcgaaagcca aagcagctgg tgcgctggtc
    38221 ggtattgaca acacctttgc cacgccgtat ctgcaacagc cgttggatat gggttgcgat
    38281 tttgtgttcc attccgctac caaatatttg tgcggccatt ccgacgtact gatgggcgta
    38341 gtcgttgcca aaaccaaaga gctggctcag cctttgcacg acatgatggt gcataccggc
    38401 gcgattgcag gcccgatgga ctgctggctg gtgctgcgcg gtatcaaaac gctggctctg
    38461 cgcatgaacg cccattgcca aaacgcactc gaaatcgcac gccgtttgga agctcatcct
    38521 gccattgaaa aagtgttcta tcccggcctg ccgtcgcacg aacattatga actcgcccaa
    38581 gcgcaaatgc ctaaaggcat cggcggcgtg gtgacggttt atctcaaaaa cgacacgcgc
    38641 gaagcggcaa acagcgtgat taaaaacatg aaactggtca aaatggcttc cagcctcggc
    38701 ggcgtggaaa gtttggtcaa ccattgctat tcccagtccc acagcggcgt gccgcataat
    38761 gtgaaaatgg aaatgggcat caaagtcggc ctgctgcgtt tctccatcgg catcgaagac
    38821 gcggacgata tttggaacga tatttccgcc gcactcgata cgactttgta aaccgtaaaa
    38881 atgccgtctg aaaccatggt ttcagacggc atttcaatta acccggccga aaatcaacgc
    38941 ttcaacatct ttgccgcctc aatagcgtaa taggtcaaaa tcccgtccgc acccgcacgt
    39001 ttgaatgcca gcaggctttc caaaaccact ttgccgccgt ccagccagcc gttggcaatc
    39061 gctgcctgca acatcgcgta ttctcccgaa acctgatagg cataagtcgg cacaccgaac
    39121 tcgtccttta cgcggcggac aacgtccaaa tacggcaaac cgggcttgac cattaccata
    39181 tccgcacctt cctgaatgtc caacgccact tcgtgcaacg cctcatcggt atttgccgga
    39241 tccatctggt aggtcttttt atctgccttg cccaaattgc ccgaactgcc taccgcatca
    39301 cggaaagggc cgtaaaatgc agaagcatat ttggcggaat acgccataat ccgcgtatgg
    39361 atatgcccgg catcctccaa cgcctcgcga atcgcaccga tacgcccgtc catcatatcg
    39421 gaaggggcaa ccacctgcgc gcccgcttca gcgtggcaca aagcctgctt gaccaaaacc
    39481 tctacggttt catcgttcat gacataaccg ttttcgtccg tcagcccgtc ctgaccgtga
    39541 accgtataag gatcgagcgc gacatccgtc ataatgccca gttcgggaaa cctctcgcgc
    39601 aaggcgcgga cagttgacgg cacgagtcct tcgggattgt acgcctcctg cgcacgctca
    39661 gttttgtttg ccgtaaccac gggaaagagt gccaacatcg gaataccgag ctttaccgcc
    39721 tcttccgccg taaacagcag cctgtccaaa ctctgacgct tcacgcccgg catagaaggc
    39781 acatcctcct cgcgcgccga cccctccaat acgaacaccg gataaatcaa atcatcggcg
    39841 gtcagcgtgt gttcgcgcat caggcggcgt gaaaaatcat ccctgcgcat acggcgcata
    39901 cgcgaagccg gaacattgcg gtaaggaaac tgcataagcc ctccaatcat cctgtcaaca
    39961 attcaaacca tacggaagcc gccgcaactt gcagacagca tcgggcggca atcaaaatcc
    40021 gttttcaccg gaaagggttt cggcccgccg aaatgccgcg ccgaaggacg gcgtcccgcc
    40081 cattaaagaa caatagcaaa acaaccgctt caggctgtca gccttcgata ttcttacgcc
    40141 acaataccaa aagtttcccg atatgctgaa caggttgcgc gtcaaccgcc tcacataagg
    40201 cagtgcagat ttcgatacgt tcggcacggt cgtcgccgaa tacgcgcact ttaatcagct
    40261 catgcgccgt caatgccgca tcggtttcct tgatgaccgc gtccgtcaga ccctgctgac
    40321 cgaccatcac aacaggatgg agatggtgcg cgcgcgcttt cagttccaaa atttctttgg
    40381 tgttcaattt ggtatccgtc attttcctac tgcttgaata aaaagaataa tgcggcattg
    40441 tacgcgattt ggcacgacat cggcaaaatt aacgtacaat acgcggtttc cattccgacc
    40501 cgcgaaccac tatggctgta cgttccaaat cctcaaaagc gtggctgcac gaacacgtca
    40561 acgaccacta cgtccatatg gcgcaaaaag acggctaccg cgcccgtgcc gcatacaaac
    40621 ttttggaaat caacgaaaaa gacaaattaa tcaaacccgg cacggtactt gccgacttgg
    40681 gcagcgcacc gggaagctgg tcgcaggttg ccgccaagct gacgggtact tccggagcag
    40741 ttttcgcctt ggacatcctg cctatggaag ccataggggg cgtctccttc attcagggcg
    40801 acttccgcga gaacgacgta ctggcacaat tcgaaacctt gttggacaac cgcccgctcg
    40861 accttgtaat ttgcgatatg gcgcccaata tgtcgggaaa cgccgtaagc gatcaggcac
    40921 gcagctttta tctgtgcgaa ctggctttag acttcgcctc gcaatacctg aaaaccggcg
    40981 gcagcttttt ggtcaaagtc tttcagggtg caggctatca ggaatacatg gcagccatgc
    41041 gcgaaatttt cggcacggtg cagacgcgca aacccgaagc ctcgcgcaat cgctccagtg
    41101 agatttattt attgggcaaa aataaacgct gacaatacag acggcgtgct ttacaatcat
    41161 ttccgtttta cacctcaatt atggagcctt gctaagtggg gaacaccttt aaatcaatcc
    41221 ttgtctgggt cgccttgggt atcggcctga tggctgcgtt caacgcttta gacggtaaaa
    41281 aagaagacaa cgggcaaatc gaatattctc agttcatcca acaggtcaac aacggcgaag
    41341 tatccggcgt caacatcgaa ggatccgtcg tcagcggcta cctgattaag ggcgagcgca
    41401 ccgacaaaag caccttcttc accaacgcgc ctttggacga caacctgatt aaaacactgc
    41461 tcgacaaaaa cgtccgtgta aaagtaacgc cggaagaaaa accgagcgcg ctggctgccc
    41521 tgttttacag cctgctgccc gtcctgctgc tgattggcgc gtggttctac tttatgcgta
    41581 tgcagacggg cggcggcgga aaaggcggcg cattctcatt cggcaaaagc cgcgcccgcc
    41641 tactggacaa agatgccaac aaagttacct ttgccgatgt cgccggctgc gacgaagcca
    41701 aagaagaagt gcaggaaatc gtcgattacc tcaaagcgcc gaaccgctat caaagcctgg
    41761 gcgggcgcgt gccgcgcggc atcctgctgg cgggcagccc gggtacgggt aagacgcttt
    41821 tggcaaaagc cattgcaggc gaagccggcg tgccgttctt cagcatttcc ggttccgact
    41881 ttgtcgaaat gttcgtcggt gtcggtgcga gccgcgtccg cgatatgttc gagcaggcga
    41941 agaaaaacgc tccctgcatc atctttattg atgaaatcga cgcagtcggc cgccaacgcg
    42001 gcgcaggttt gggcggcggc aatgatgagc gcgagcaaac attaaaccaa ttgttggttg
    42061 aaatggacgg ttttgagagc aatcagactg tgattgtgat tgcggcaacc aaccgccccg
    42121 acgtactcga tcctgcgctg caacgccccg gccgtttcga ccgccaagtg gttgtccccc
    42181 tgccggacat ccggggacgc gaacagattt tgaacgtcca ttctaaaaaa gtgcctttgg
    42241 acgaatctgt ggatttattg tccctcgcgc gcggcacgcc gggtttttcc ggcgcggatt
    42301 tggcgaactt ggtcaacgaa gccgccctgt ttgccggccg ccgcaacaaa gtcaaagtcg
    42361 atcagagcga ttttgaagac gccaaagaca aaatctatat gggtccggaa cgccgcagta
    42421 tggtgatgca cgaagacgaa aaacgtgcga cggcgtatca cgaatccgga cacgcgattg
    42481 ttgccgaaag cctgcccttt accgaccccg tccacaaagt aaccattatg ccgcgcggac
    42541 gtgcgctggg tctgacttgg cagcttccgg agcgcgaccg catcagtatg tataaagatc
    42601 agatgttgag ccagctctcc atcctgttcg gcggacggat tgccgaagac atcttcgtcg
    42661 gacgcatctc caccggcgca tcaaacgact ttgaacgcgc aacccaaatg gcgcgcgaaa
    42721 tggtaacgcg ctacggcatg agcgacaaaa tgggcgtgat ggtttatgcg gaaaacgaag
    42781 gcgaagtctt cttgggacgc agcgtaaccc gttcgcaaaa catttccgag aaaacccagc
    42841 aggacatcga cgcggaaatc cgccggattt tggacgagca atatcaggtt gcctacaaaa
    42901 tcctcgatga aaaccgcgac aagatggaaa cgatgtgcaa agccctgatg gaatgggaaa
    42961 ccatcgaccg cgatcaggta ctggaaatca tggcgggcaa acaacccagt ccgcccaagg
    43021 attacagcca caacctgcgc gagaatgcgg acgcggcgga agataacgcg ccgcacgctc
    43081 cgactcggga agaaaccgaa gcacctgccc cggcagacac cgcttcgaca gagtccgagc
    43141 agcagcctga aaacaaggct taacttcccg aacaaacggc agcccgcaag ctgccgtttt
    43201 cccatcctgt tttcagacgg cattttccgc tcaatgccgt ctgaaacgcc aaagccgtat
    43261 cggtttttgc gaatctgcga tatggcggtt taaattgaaa tcagaacggg gcaaggctgt
    43321 accggtttaa aattaaaccg ctataatacc gcccttcgaa tacacgcccg cattggtgat
    43381 gcccgtgaaa aaagttgaaa aaaacatctt ggtgctgcac ggcgcggaca aaatgttcga
    43441 gctggtcgat aaggttgagg attacccgaa ctttctgccg tggtacagca aaaccgaggt
    43501 catcggacgc aacggcaacg aactgaaggc gcggctgttt atggactata tgcacgttcg
    43561 ccaatcattc gccacgcaca accgcaacat tccgggcagg gaaatccgta tggaactgct
    43621 cgaaggtccg ttcaaaacct tacgtggaac gtggaaattt atcgatttgg gcgacgatat
    43681 gtgcaaaatc gaattcaatt tggaatacga tttttccaat gccgttttgt ccgccttaat
    43741 ttcccccgtc ttcaaccacc tttccgccac gctggtcgaa gcgttcgtca aagaggcaga
    43801 ccgccgttat gcttgaaatt gagattgtgt acgggctgcc cgaccgacag gttttgaaaa
    43861 ccatgcagct tgccgaggga acaaccgtcc gcgccgccgc actgcaaagc ggtttggacg
    43921 gcatatttga agatttaaac ctgcattccg cgcctttggg cattttcggc aaagccgtca
    43981 aagacgacac gccgctgcgc gacggcgacc gcatcgaagt gtaccgcccg ctgttgatcg
    44041 accccaaaga agcgcgccgc aaacgcgttc aaaatcaaga agaataacca tgccgtctga
    44101 agccttcaga cagcattcga cagaaacacg gaaaatatca tgtcaaacaa aatcaaaatg
    44161 cttgtcggat tgggcaaccc cggcaaagaa tacgaacaaa cccgacacaa tgcgggtttt
    44221 tggttcctcg acgaactggc ttggaagtgg aaggcttcat ttaaagaaga aaaaaaattc
    44281 ttcggcgaag tcgcccgtgc cgccctgccc gacggcgatg tctggctgct caagccgacc
    44341 acgttcatga accgttccgg acaggcggtt gccgcccttg cgcagttcta caaaatcaaa
    44401 cccgaagaaa tcctcgtcgt ccacgacgaa ctcgacattc cctgcggacg gatcaaattc
    44461 aaactcggcg gcggcaacgg cggacacaac ggcttgaaag acattcaggc aaaactcggc
    44521 acggcagact attaccgcct gcgcctcggc atcggccacc cgggcgaccg caacctcgtc
    44581 gtcggctatg tcctgaacaa acccagtacg gaacaccgcc gacagattga cgatgccgtc
    44641 gccaaatccc tgcaagccat acccgacatc cttgccggca aatgggaaga agcaacccgc
    44701 ttcctgcaca gcaaatgacc cgatgccgtc tgaagccctt tcagacggca tgttcccgat
    44761 ttccatatcc gaacagtcat gaccgaactc aagcagctta tccaaaccga atccatcccc
    44821 gtcatcgaag aaaccctcga tttcctgctg tacgaatgca gcatcgacga cgcaccgtcc
    44881 gtcgaagaag tggcacaatg gcgcgacata cttgccgcac gcggcggcaa attcctgcgc
    44941 ctgtccaaaa tctgccaaac gtggctggac gaggaggcgg catgaagctg ccgcgcaacc
    45001 gcttcagcct gctttccgca ttgtggtttg ccggcggcat ctattcgctg ctcttcaaag
    45061 ctgccgacac cgcgccgccg ccgtttccgc atttcgacaa agcagcacac cttgccctgt
    45121 ttttcgcaca aatctggctt ttgaccaaag cattcaaaac cggaaaactt cccatcccct
    45181 accgcagcct gatggtcttt gccctctgtt tcgccctctt cagcgaatgc gcgcaggcat
    45241 ggtttaccgc aacgagaacc ggcagtttgg gcgatgttct tgccgatatg gcgggtacgg
    45301 ttctcgcact ctttgccgcc cgcgccgccg accgcccgga ctgaatcggt ttatttcccc
    45361 caaacaggat gcacctcttc ccgatacatc ctatttttcc ccttacatat caatcccatc
    45421 tcataaacaa aaccgacaaa tcgtttacaa tatatttaca ctacatcaga ttacaaatat
    45481 actcgaacca gttgtagaat cggcggttcc acaaaccgtt tcggattgca cgacacaaaa
    45541 gaaaaaccga ttttattgcc ttaaaggagt attcatgaac ctgcatgcaa aggacaaaac
    45601 ccagcatccc gaaaacgtcg agctgctcag tgcgcagaag ccgattaccg actttaaggg
    45661 cctgctgacc accattattt ccgccgtcgt ctgtttcggc atttaccaca tcctgcctta
    45721 cagccccgat gccaataaag gtatcgcgct gctgattttc gttgccgcac tttggtttac
    45781 cgaagccgtc cacattaccg taaccgcact gatggtgccg attctcgccg tcgtactcgg
    45841 tttccccgac atggacatca aaaaggcgat ggctgatttt tccaacccga ttatctacat
    45901 ttttttcggc ggcttcgcgc ttgccaccgc cctgcatatg cagcggctgg accgtaaaat
    45961 cgccgtcagc ctgttgcgcc tgtcgcgcgg caatatgaaa gtggcggttt tgatgttgtt
    46021 cctcgttacc gcctttctgt ccatgtggat cagcaacacc gccaccgccg cgatgatgct
    46081 gcctctggca atgggtatgc tgagccacct cgaccgggaa aaagaacgca aaacctacgt
    46141 cttcgtcctg ctcggcatcg cctattgcgc cagcatcggc ggcttgggca cgctcgtcgg
    46201 ctcgccgccc aacctgattg ccgccaaagc cctaaatctg gacttcgtcg gctggatgaa
    46261 gctcggcctg ccgatgatgc tgttgattct gcccttgatg ctgctctccc tgtacgtcat
    46321 cctcaaacct aatttgaacg aacgcgtgga aatcaaagcc gaatccatcc cttggacgct
    46381 gcaccgcgtg atcgcgctgt tgattttcct tgccacagcc gccgcgtgga tattcagctc
    46441 caaaatcaaa accgccttcg gcatttccaa tcccgacacc gttatcgccc tgagtgccgc
    46501 cgtcgccgtc gtcgtcttcg gcgtggcgca atggaaggaa gtcgcccgca ataccgactg
    46561 gggcgtgttg atgctcttcg gcggcggcat cagcctgagc acgctgttga aaacatccgg
    46621 cgcgtccgaa gccttgggac agcaggttgc cgccaccttt tccagcgcgc ccgcattttt
    46681 ggtgatactc atcgtcgccg ccttcattat ttttctgacc gagttcacca gcaacaccgc
    46741 ctccgccgca ttgcttgtac cgattttctc cggcatcgct atgcagatgg ggctgcccga
    46801 acaagtcttg gtattcgtca tcggcatcgg cgcatcttgt gccttcatgc tgccggttgc
    46861 cacaccgcct aacgcgattg tgttcggcac aggcttaatc aagcaacgcg aaatgatgaa
    46921 tgtcggcata ctgctgaaca tcctctgcgt agtattggtt gctctgtggg cttatgctgt
    46981 actgatgtaa accatcgacc taaacaacaa gaccgtctga aagaatattt ttcagacggc
    47041 cttgaagttt gtcgttcaga cacaatttgt cgaatcattc aaaaccggat tctaacgaaa
    47101 ggaaacccat gattatcctg cacaccaaca aaggcgacat caaaatcgaa ctcgatttcg
    47161 acaaagcccc cgttaccgcc aaaaacttcg agcaatacgt caaagacggc ttctacgacg
    47221 gcgtaatctt ccaccgcgtc atcaaaggct tcatgattca aggcggcggc atggatgaaa
    47281 acatgaacga aaaagaaacc cgcgatccga ttcaaaacga agcgtccaac ggcctgccca
    47341 acgataaata caccatcgcc atggcacgca cttccgaccc ccattccgcc agcgcgcaat
    47401 tcttcatcaa cactgccgac aacgctttcc tgaacttccg ttctaaagag ctgtacggca
    47461 aaaccgtcgt ccaagactgg ggctatgccg tattcggtaa agtcgttgac ggttttgatg
    47521 ttgtcgatgc catcgaaggc gtatccacca aacgccacgg ctaccatgac gacgtaccga
    47581 gcgaacctgt cgtcatcatt aaagccgaag cggtataaac cggcaatccg aaagcagcct
    47641 gcacaaaggc tgcttttttt gtttcaggcg gcataaaatc caagcacata tgccgtctga
    47701 acacctcccg caagccttcg gacaatgttg caacgtgtta aaatagcctc ctttcctgaa
    47761 accacaacaa ggaaaccatc atggcaaata ttgcccgcga catcttcaaa gcctacgaca
    47821 tccgaggcat tgtcggcaaa accctgactg acgatgccgc ctatctcatc ggcagggcca
    47881 tcgccgccaa agccgccgaa aaaggcatga cccgcatcgc gctcggacgc gacggacgct
    47941 tgagcggtcc cgaactgatg gagcacatcc aacgcggctt taccgacagc ggcatcgacg
    48001 tcctcaacgt cggtatggtc gccacgccca tgctctattt tgccgctatc aacgaatgcg
    48061 gcggcagcgg cgtgatgatt accggcagcc acaatccgcc cgattacaat ggtttcaaaa
    48121 tgatgctcgg cggcgacacg ctcgcaggcg aagccattca agaactttta gccattattg
    48181 aaaaagacgg ttttgttgcc gccgacaaac aaggcaacgt aaccgaaaaa gacatctccg
    48241 gcgaatacca caaccacatc gtcggccaca tcaaactcaa acgccccatg aaaatcgcca
    48301 tcgacgcagg caacggcgta ggcggcgcat tcgcaggcaa actctacaaa ggcttgggca
    48361 atgaagttac cgaacttttc tgcgaagtgg acggcaactt ccctaatcac caccccgacc
    48421 cttccaaacc gaaaaacctg caagacctca tcgtcgagct caaaaacggc gaggccgaaa
    48481 tcggcttagc atttgacggc gatgccgacc gcttgggcgt ggttaccaaa gacggcaaca
    48541 tcatctatcc ggaccgccaa ctgatgcttt tcgcgcaaga cgtgttgagc cgcaatccta
    48601 aagccaaagt gattttcgac gtcaaatcca cccgtctgct tgcgccttgg attaaagaac
    48661 acggcggcga acctgtgatg gaaaaaaccg gccacagctt tatcaaatcc tccatgaaaa
    48721 aaaccggcgc gccggttgcc ggcgaaatga gcggacacat cttcttcaaa gaacgctggt
    48781 tcggcttcga cgacggtctg tacgccggcg cacgcctctt ggaaatcctg tccgcctccg
    48841 ataatccgtc cgaagtgttg aacaacctgc cgcaaaacat ttccacgccc gaactcaaca
    48901 tcgccctgcc cgaaggcagc aacggccatc aggttatcga cgaactcgcc gccaaagccg
    48961 aattcgaagg cgcaaccgaa atcatcacca tcgacggtct gcgcgttgaa tttcccgacg
    49021 gcttcggtct gatgcgtgct tccaatacca cgccgattct ggtgttgcgt tttgaggcgg
    49081 atacgcaagc agccatcgag cggattcaaa accaattcaa agccgtcatc gaaagcaatc
    49141 caaatttaag ctggccgctg taaacatcga aaaaatgccg tctgaaggct tcagacggca
    49201 tttttcacaa atagttaatc atatcttagt agattggatt tggcttaaaa atctccgcgt
    49261 ccgttcgtgt ttggggtgat cgaacaactc tttcgggctg ccttgttcga caataacgcc
    49321 gccgtccatc acgacaacgg tagtcgccac ttcaagcgca aacttgattt cgtgggtaac
    49381 gacgaccatc gtccaacctt cccgcgccaa ttccttcatg gcgttcaaca cgtcttgcac
    49441 caactcgggg tcaagcgcgg aagtgggttc gtcaaacaac atcagctcgg gctgaatcgc
    49501 caatgctcgg gcaatgccga cgcgctgctg ctgaccgccg gaaagctggt agggatacaa
    49561 atccactttg tcgcccaagc cgactttttc cagcagtttc agagcctctt cgcgcgcttg
    49621 ggcggcaggc ttgccctgta cggcaaccgg tccttccatc acgttttcca aggcggtttt
    49681 gtgcggaaag aggttgtatt gttgaaacac catgcctgat ttgcggcgca gtgccaaaat
    49741 atcgtgtttg cttggttttt tagaaaaatc gattttcagc ggtcgctcgt tgtcgaactc
    49801 gatttgtccg tcttcgggca tttccaacgc gtttaggcag cgcagaaacg tcgttttgcc
    49861 tgagccggaa ggcccgagga tgacgaccac ctgccctttg cacacatcca aatcgatgcc
    49921 gcgcaaaatg gtattttcgc cgaaggtctt atggatattg cggattttaa tcatgacaac
    49981 tcctcatttg gcgacatagc ggtcgaaacg tttttccaaa cgcgcctgaa tcagaaacag
    50041 cactttgcag aaacaccagt aaaccaaagc ggcttcgata tagacgggca aaaagtcata
    50101 agtgcggttt gccgtttcct gcgcgacgcg gaataattcc gttaccgtca cgactgccgc
    50161 gagcgaggtg tttttaaaca aaccgataaa ctcgttgctc aaaggcggca cggcaacgcg
    50221 gaatgcctgc ggcgcgacaa tgcggcggaa cgtctgcata taggtcatgc cgatggagaa
    50281 acctgcttcc cattggcctt taggtacgga caaaattgcc gcgcgtatgg tttcggaagc
    50341 atatgcgccg acattgagcg aaaagccgat gatggcggca ggaatcgggt cgatatagat
    50401 gccgacggaa ggcagcccgt aaaacacaat cacaagctga accaacagcg gcgtaccgcg
    50461 aatgacggaa atataaaatt ccaccaattt cagcaggatt ttccgcacga tgccgccggc
    50521 gggcataatc cgcaccaaag ccacggctac cgcaatcatc ataccgataa cgaaagaagc
    50581 tgccgccaaa ggcagagaga ccgcgaagcc ggctttgacc ataggcaaaa acgcgctgac
    50641 aatcatatcg gcgcgtgttt ccgtcataaa cggcagcgaa gcaaggaaat tattgaacac
    50701 tgatgtcttt tccgaagaat tgttcgccca gttttttcag cgtaccgtcg gctttcagct
    50761 cgttgattgc cgtactgaat ttcgccacgg cttcgtcatt gcccttgttg acaatcaggc
    50821 cggaaccgac tttttcatcg gcaggtgcgg accaaacgat tttcacgccc gcattcgggt
    50881 ttttcttcag gtaatccaat actgccaatt cgtcgttcag ggttgcatcg gcacgttttt
    50941 gttcaatcag ggtcagcgat tgcgccaaac cgtcaacagc caccaaatct gcgcctgcag
    51001 ctttggcttt ttcgccgtag ttgctggtca gggattgtgc ggttttcacg cctttgatgt
    51061 cgtcgataga tttgatgttg ctgtcgttac gggcaaccaa tacggcaccg ctccagctgt
    51121 aaggatcgga tttgtcgaat gtcgcttggc gttcggggct ggtcagaccg acttggtttg
    51181 ccaccacgtc gaaacgcccc gccttcaaac ccgccatcat cgaatcccat tgcgtttctt
    51241 taaactcgac tttcacgccc agtttttccg ccacggcgcg ggttacttcc acatcgtaac
    51301 cggtcagttt gccgtccttg tcgtggtagg taaacggtgc gtaagtgcct tccgtgccga
    51361 cggtaaccgt gcctttattg ttgatgcgct cgattaaaga accggaaact gccgattgtg
    51421 caggcgcgga agatgctccg ctgccgcctt ccgaaccgcc gcaggccgcc aaaaccaatg
    51481 cggcaatacc gccgagtacg aattttttca acatcattct ctcctgtaaa aagtgtgcaa
    51541 gtgcggcatt ctaagtgaaa ttgcggcaaa cacaaagaat ccttttttta tatggttatt
    51601 ccaaatcgga atggaaaaga tatgccgaag ccgccggaag cggaaataaa gggggcggga
    51661 aaggcggcgg gcggcacgaa accgaaaagc ggcatcaggt atgcaaacgg cggattcacg
    51721 cacgccgttc cggtaaaaat ccgcctgccg attcggtgct ataatccatc cgaaacagat
    51781 gacaccaaaa aggaacatct catgaacacg ccccaatccg ccatcattcc cgaccacgcc
    51841 caagccggca tctttatcga agccgacttc gccgccaacc gcctcaacga tattaaagcc
    51901 gcctgccgcg cttcgctcga cgcgttaact gccttgaaag cccgctttcc ggacgatatt
    51961 ttgggtctga ccatcgcctt cggcagcaaa gcctgggcaa cattcggaca tacggacgaa
    52021 ggcagtgaaa tcaaaccctt ctccgaaatg ggcaacgggc ttgcgccgtc tacgcagcac
    52081 gatatgtcca tccacatcca atctttccgc caaaacgccg cctacgcgct tgcccaatcc
    52141 gttttgggcg cattcggcga cagcatatgc gtcgcgtccg aagaacacgg tttgcgcctg
    52201 tatcaggatc gcgggctgga cggtttcgtc gacggcacgg aaaacccgca gggcgatgaa
    52261 aatgtccgcg aagtcgccat catccccgaa ggaaaacccg atgcgggcgg cagctatgtc
    52321 ctgctgcaaa aatacctgca cgatttgaaa aaatgggatg ccgtccccgt cgccgaacag
    52381 gaggcctcgg tcggacgcgg caaggaaacc gacgacgaat tcggccgcga tgtccgcctg
    52441 cccgattcgc acctcggccg cgtcaacctg aaagaaaacg gagtcggcct gaaaatcgtc
    52501 cgccgcagcc tgcccttcgg caaaatcagc ggcgaacacg gtttgatgtt taccgcatac
    52561 tgccgcacgc tgcacaatat cgaagcgcaa ttattgagta tgttcggcga tacggacggc
    52621 aaaaccgacc tgctgctccg acacctctcc gccgccgttt cgggcggata ctactacgcg
    52681 ccgtctgtcg aacgcctgca aaacctctaa gccggacgat gccgtctgaa accatttttc
    52741 agacggcatt ttcc
//
LOCUS       DAXHTY010000003        40491 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXHTY010000003 DAXHTY010000000
VERSION     DAXHTY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40491
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.003"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            55..3669
                     /gene="recB"
                     /locus_tag="ACFNMK_000138"
     CDS             55..3669
                     /gene="recB"
                     /locus_tag="ACFNMK_000138"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC6363487.1"
                     /translation="MSAPIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQTDLPQARDAALASWQNICGNLAELKETFWRIHPLLNGNSYRKNSFAELF
                     DALEQKALSPDLPFLEAKLHERLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFRRD
                     LDALEEAEEATIIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRATDYCADEIAHALNEAAECRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRQTYDREKDGIAMLKRNWRRLTDNAPAGTDFAFTEDAPPPAAYRGNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACQKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICVIDYKSNHLGADASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            3708..4067
                     /locus_tag="ACFNMK_000139"
     CDS             3708..4067
                     /locus_tag="ACFNMK_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6363488.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPLSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAKAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            4199..4678
                     /locus_tag="ACFNMK_000140"
     CDS             4199..4678
                     /locus_tag="ACFNMK_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC6363489.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAAHRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(4953..6332)
                     /gene="radA"
                     /locus_tag="ACFNMK_000141"
     CDS             complement(4953..6332)
                     /gene="radA"
                     /locus_tag="ACFNMK_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC6363490.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTDGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(6510..7574)
                     /gene="hemE"
                     /locus_tag="ACFNMK_000142"
     CDS             complement(6510..7574)
                     /gene="hemE"
                     /locus_tag="ACFNMK_000142"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC6363491.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEDDIAKLHVPDMEKLQYVFDAVSSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEAARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(7649..8866)
                     /locus_tag="ACFNMK_000143"
     CDS             complement(7649..8866)
                     /locus_tag="ACFNMK_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC6363492.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLYAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            complement(8863..10137)
                     /locus_tag="ACFNMK_000144"
     CDS             complement(8863..10137)
                     /locus_tag="ACFNMK_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC6363493.1"
                     /translation="MPSETSSPRKENETEVHIPAAPFIVKQSGSNALAVCALVLAALG
                     LGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDRLD
                     SGVKANGEQILMTQKAYRELTKGRADWLVDETDTILNLAAQQLVLTGNIQTAVGVLEH
                     IDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLETAVFGLPLILDG
                     VLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENL
                     RLRLLDVRTALMQRNSEVYQGDLNNAEAAVRQYFDAKSPATQSWLKELAELKALDVRM
                     TADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPANENTASEPAAASDVKTIEAP
                     SLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            complement(10212..10973)
                     /locus_tag="ACFNMK_000145"
     CDS             complement(10212..10973)
                     /locus_tag="ACFNMK_000145"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC6363494.1"
                     /translation="METVKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRALERCLGRTV
                     IAPEDGNDSEAVLRLTVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(10991..11413)
                     /locus_tag="ACFNMK_000146"
     CDS             complement(10991..11413)
                     /locus_tag="ACFNMK_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC6363495.1"
                     /translation="MKKMFLSAVLLLSAATRTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            complement(11652..11972)
                     /locus_tag="ACFNMK_000147"
     CDS             complement(11652..11972)
                     /locus_tag="ACFNMK_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC6363496.1"
                     /translation="MNFQDYLATFSSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(11995..12621)
                     /gene="upp"
                     /locus_tag="ACFNMK_000148"
     CDS             complement(11995..12621)
                     /gene="upp"
                     /locus_tag="ACFNMK_000148"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC6363497.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(12727..13038)
                     /gene="grxD"
                     /locus_tag="ACFNMK_000149"
     CDS             complement(12727..13038)
                     /gene="grxD"
                     /locus_tag="ACFNMK_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC6363498.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(13139..13354)
                     /locus_tag="ACFNMK_000150"
     CDS             complement(13139..13354)
                     /locus_tag="ACFNMK_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC6363499.1"
                     /translation="MEAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            complement(13380..14159)
                     /locus_tag="ACFNMK_000151"
     CDS             complement(13380..14159)
                     /locus_tag="ACFNMK_000151"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6363500.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     AEVSAIAEVYEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            complement(14164..14514)
                     /locus_tag="ACFNMK_000152"
     CDS             complement(14164..14514)
                     /locus_tag="ACFNMK_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC6363501.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(14518..15495)
                     /locus_tag="ACFNMK_000153"
     CDS             complement(14518..15495)
                     /locus_tag="ACFNMK_000153"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC6363502.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(15529..16659)
                     /locus_tag="ACFNMK_000154"
     CDS             complement(15529..16659)
                     /locus_tag="ACFNMK_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6363503.1"
                     /translation="MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            16827..17528
                     /locus_tag="ACFNMK_000155"
     CDS             16827..17528
                     /locus_tag="ACFNMK_000155"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC6363504.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFTAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDDLLVGKAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            17671..19464
                     /gene="lepA"
                     /locus_tag="ACFNMK_000156"
     CDS             17671..19464
                     /gene="lepA"
                     /locus_tag="ACFNMK_000156"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC6363505.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            19607..20626
                     /gene="lepB"
                     /locus_tag="ACFNMK_000157"
     CDS             19607..20626
                     /gene="lepB"
                     /locus_tag="ACFNMK_000157"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC6363506.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(20845..20994)
                     /locus_tag="ACFNMK_000158"
     CDS             complement(20845..20994)
                     /locus_tag="ACFNMK_000158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363507.1"
                     /translation="MNWQAVKTGKTKHAGNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(21040..21945)
                     /locus_tag="ACFNMK_000159"
     CDS             complement(21040..21945)
                     /locus_tag="ACFNMK_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6363508.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEVEPVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
     gene            complement(22216..22349)
                     /locus_tag="ACFNMK_000160"
                     /pseudo
     CDS             complement(22216..22349)
                     /locus_tag="ACFNMK_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(22511..23443)
                     /gene="cysK"
                     /locus_tag="ACFNMK_000161"
     CDS             complement(22511..23443)
                     /gene="cysK"
                     /locus_tag="ACFNMK_000161"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC6363509.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAMAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(23637..24242)
                     /locus_tag="ACFNMK_000162"
     CDS             complement(23637..24242)
                     /locus_tag="ACFNMK_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363510.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(24239..25090)
                     /gene="dapF"
                     /locus_tag="ACFNMK_000163"
     CDS             complement(24239..25090)
                     /gene="dapF"
                     /locus_tag="ACFNMK_000163"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC6363511.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            25227..25523
                     /locus_tag="ACFNMK_000164"
     CDS             25227..25523
                     /locus_tag="ACFNMK_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363512.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLSPKNI"
     gene            25537..26205
                     /locus_tag="ACFNMK_000165"
     CDS             25537..26205
                     /locus_tag="ACFNMK_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6363513.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(26278..28398)
                     /gene="pnp"
                     /locus_tag="ACFNMK_000166"
     CDS             complement(26278..28398)
                     /gene="pnp"
                     /locus_tag="ACFNMK_000166"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC6363514.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNLEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLKVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(28635..29498)
                     /locus_tag="ACFNMK_000167"
     CDS             complement(28635..29498)
                     /locus_tag="ACFNMK_000167"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC6363515.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            29697..30560
                     /gene="rfbD"
                     /locus_tag="ACFNMK_000168"
     CDS             29697..30560
                     /gene="rfbD"
                     /locus_tag="ACFNMK_000168"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC6363516.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALSAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSSLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(31035..31268)
                     /locus_tag="ACFNMK_000169"
     CDS             complement(31035..31268)
                     /locus_tag="ACFNMK_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC6363517.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(31434..31634)
                     /locus_tag="ACFNMK_000170"
     CDS             complement(31434..31634)
                     /locus_tag="ACFNMK_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC6363518.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(31806..32681)
                     /gene="xerD"
                     /locus_tag="ACFNMK_000171"
     CDS             complement(31806..32681)
                     /gene="xerD"
                     /locus_tag="ACFNMK_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC6363519.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTCLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(32733..33173)
                     /locus_tag="ACFNMK_000172"
     CDS             complement(32733..33173)
                     /locus_tag="ACFNMK_000172"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC6363520.1"
                     /translation="MDVKYEFTLPSSSSADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            33494..34432
                     /locus_tag="ACFNMK_000173"
     CDS             33494..34432
                     /locus_tag="ACFNMK_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC6363521.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            34561..34854
                     /gene="hfq"
                     /locus_tag="ACFNMK_000174"
     CDS             34561..34854
                     /gene="hfq"
                     /locus_tag="ACFNMK_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC6363522.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPASTLVQVETVQQPAE"
     gene            34939..35505
                     /locus_tag="ACFNMK_000175"
     CDS             34939..35505
                     /locus_tag="ACFNMK_000175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6363523.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNNPPYLLAFEKLRQK"
     gene            35559..35933
                     /locus_tag="ACFNMK_000176"
     CDS             35559..35933
                     /locus_tag="ACFNMK_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC6363524.1"
                     /translation="MQYLIVKYSHQIFVTVTILLFNIRFFLLWKNPEKPLAGLWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSVKFYAV
                     YLLAICCVACIVYLAKTKVLPF"
     gene            35943..36437
                     /gene="folK"
                     /locus_tag="ACFNMK_000177"
     CDS             35943..36437
                     /gene="folK"
                     /locus_tag="ACFNMK_000177"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC6363525.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTDMDGISLLSLLNRIEADFGRERSFRNAPRTLDLDII
                     DFDGLSSDDPRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKQLGNQGIRL
                     LPDK"
     gene            complement(36564..37043)
                     /locus_tag="ACFNMK_000178"
     CDS             complement(36564..37043)
                     /locus_tag="ACFNMK_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC6363526.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
     gene            complement(37263..38000)
                     /gene="ubiE"
                     /locus_tag="ACFNMK_000179"
     CDS             complement(37263..38000)
                     /gene="ubiE"
                     /locus_tag="ACFNMK_000179"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC6363527.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(38043..38411)
                     /locus_tag="ACFNMK_000180"
     CDS             complement(38043..38411)
                     /locus_tag="ACFNMK_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC6363528.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            38531..40150
                     /gene="mnmC"
                     /locus_tag="ACFNMK_000181"
     CDS             38531..40150
                     /gene="mnmC"
                     /locus_tag="ACFNMK_000181"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC6363529.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITPAEAEKIAGIPLNTPYAEPLCGLFWQYGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSPR
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(40264..40381)
                     /locus_tag="ACFNMK_000182"
                     /pseudo
     CDS             complement(40264..40381)
                     /locus_tag="ACFNMK_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
BASE COUNT         9808 a        11705 c         9677 g         9301 t
ORIGIN      
        1 atcctgattt ttgttaatcc actataccgt ccaaccgttt tccgagcgcg cactatgtct
       61 gcacccatcc aagcatttga cccgctaacc gtccccattt ccggcaccaa cctgattgag
      121 gcctctgccg gcaccggaaa aacctacggc attgccgccc tgtttacgcg cctgatcgta
      181 ttggaacaaa aaaacgtcga gcgcgtattg gtcgttacct tcaccaaagc cgccaccgcc
      241 gagttgaaaa cacgcctgcg cgcacgtttg gacgatgtgt tgcaggtttt agaaagtaaa
      301 gaaattgcca aacttggaga cgacacactt tcagacggca tcgccgccta ctgcgccgaa
      361 caccacaaag gcgacacctt cctgcccgca ctcttaaaac aggctttgca aaaagagggc
      421 cggacgcgtc tgattgtccg cctcaaagcc gccatcgggc aattcgacaa cgccgccatc
      481 tataccatcc acggcttctg ccagcgcatc ctgcgcgact acgcgttttt gtgccaagca
      541 ccgttcgatg tcgaactgac cgaagaagac ggcgaccgcc tgcttgtccc ggcgcaagat
      601 ttttggcggg aacgcgtcag caatgatccg gtgcttgccg cattggcgtt taaacgcaaa
      661 gctgtgccgc aaaccgtcct tgcccaaatc cgcgcctacc tgtcccgccc gtacctgaat
      721 ttccgccgtc cgcaaaccga tttgccgcaa gcccgggatg ccgctttggc aagctggcaa
      781 aacatttgcg gaaatctggc tgaattaaaa gaaacttttt ggcgcatcca tcccctttta
      841 aacggcaatt catatcggaa aaacagtttt gccgaactgt ttgacgcatt ggaacaaaaa
      901 gccctgtcgc cggatttgcc ctttttggag gcaaagctgc acgaacggct cttgaaactt
      961 gcgtccgaca aactcgaagc cggactgaaa aaaggcaaaa cgcccgatgc ggcagtattt
     1021 gccgaattgc agaaactggc agacttcagg cgtgatttgg acgcactcga agaagcggaa
     1081 gaagcaacaa taatccggct gcaactggat ttaatcggat acctcaaccg cagccttgcc
     1141 gagatgaaaa aatcgcgccg cgaacgcggt ttcgacgacc tgctgctcga tgtccacacc
     1201 gcgctgaccg acaatccgca cgccgaaacc ctcgcccgcg ccgttgccga aaactgggaa
     1261 accgcgctga tcgacgagtt ccaagacacc gacccgctgc aatacgaaat cttccaaaaa
     1321 atcttcatcg cccaaaaccg accgctgttc ctcgtcggcg atcccaaaca ggcgatttac
     1381 agctttcgcg gagcggacat ttacgcctac cttcaggcgg cggaagacgc gcggcaccgc
     1441 tacacgctcg ccaccaacta ccgcagccac gccgcgctta tcggcagcat aggcgcgctg
     1501 ttccgcctca aagaacgccc gttcgttttg gaaaacatcg gctattcgga agtcggtgcg
     1561 gcgcgtgccg aaagcaggtt gtcccccgaa cgtcctgccg tacaggttcg ttggctgcac
     1621 gaaaacgaca atgaaaaagc caacaaagac gttttgcgcc gccgtgccac cgactattgc
     1681 gccgacgaaa tcgcccacgc gctcaacgaa gccgccgaat gccgtctgaa tttcaaaggc
     1741 tgcccgttgc agtcgggcga tattgccgtg ctggtacgca cgcacaacga ggcggtgatg
     1801 gtttccgctg ccctgaaaaa acggcaggtg caaagcgtcc tgctttcgcg cgaatccgtg
     1861 ttcgcctcgc ccgaagccgc cgccctttcc gcactcatcg gcttctggct cgaaccgcgc
     1921 cgcgccggaa cgctgcgctt tgtcctgaca agcggcatat tcggttatga cgcgcagcaa
     1981 ttgcacgact tcaaccaaaa cgaaagcgag attttgcatt gggcggaatc tgcccgaacc
     2041 gcgctcgaca actggaatca atacggcatt ttcgccgcca tgcagcaatt ttcccaaaca
     2101 cacggcatcg aaacgcgcct cttaagccgg aacaacgggc gcagcctgac caattatttc
     2161 caactgctcg aactgcttgc cgccgaagac gcgcaaaacc gcaaccccgc cgcgctgcac
     2221 aaatggttgc gcgaccaaat cagcctggcc ggcaacaaca gcggcgacaa ccgcgccatc
     2281 cgtctggaaa gcgacgaaga tttggtcaaa atcgttacca tgcacgcctc gaaaggtttg
     2341 cagtatccgc tggtatactg cccgtttgcg tgggacgcgc aagataccgg accgtccgac
     2401 tggcaaatcc tccaccaaag cgcaaaccga accgaactgt tggcaaaggc gcaactgtcg
     2461 gaagacgaac agaagcaata cgccgacgaa gaaatggcgg aacgcctgcg cctgctttat
     2521 gtcgcgctga cgcgtgccga ggaacagctc aacatctacg ccgcctattc ttccgatacc
     2581 gccgacaacc ccctcgccta cctgattgaa ggcttgccgc aagacagccg cgaaaccgtc
     2641 cgccaaacct atgaccggga aaaagacggc atcgcgatgc tcaaacgcaa ctggcggcgt
     2701 ttgacggaca atgccccggc cggcacggat ttcgccttca cagaagatgc gccgccccct
     2761 gccgcctatc gcggcaacgc cggtcaaacc gccgaatttg ccgcaaacag cattcccgaa
     2821 cgcagattcc gatttgtccg ccacaccagc tttaccgcct taagccgcca tacccaaacg
     2881 cccgacggcg gcgaagaaga tgcctgcccg tccttggatg ccgccgaaac ctcggtgccg
     2941 gcgatgccgt ctgaaacgcc gacggcttca gacggcatat cgatacacga ttttccgaaa
     3001 ggcacgcagg cggggctgtg cctgcacgaa attcttgaag atttcaaatt cggacaagca
     3061 gccgccggac aggaaactct cattgccgac aagctgaaaa aatacggatt tgaagaaata
     3121 tggctgcctg ccgttgccga aatggcggaa gcctgccaga aaacgccgct gacgggggca
     3181 tactgcctgt ccgacatccc gcccgagtgc cgctgtcccg aaatgggctt taccctccac
     3241 accgaagact tcggcctcaa acggctgcgc gactggtttg cccgcgacga tatccgattg
     3301 cccgaagtct gccgtgccgc cgccgaaacg ctcgacttcc acaccgtcaa cggcttttta
     3361 aacggcttta tcgatatggt ctgccaagac cccgacggca atatctgcgt catcgactac
     3421 aaatcaaacc atctcggcgc ggacgcatcc gcctacacgc aacaggcgat ggacgaagcc
     3481 gtcgcgcacc accactatta ccttcaggcg ctgatttacg ccgttgccgc cgcacgctac
     3541 ttcaaactgc gcggacaacc gcccgccgcc gtttccgtcc gctacctgtt tttgcgcgga
     3601 ttggatggca aaggcggcgg cgtttggcgt tgggacatag atgccgccgc tttggaacag
     3661 ataaaataac ttcccgtccc ttaagacgac aaccgcagga gaaccctatg aatatcaaac
     3721 acctgattac cgccgcactc attgcctcag ccgcctttgc cgcgcaggca gccccgcaaa
     3781 aacccctatc cgccgcccaa accgcgcaac atccggccgt ttggatcgat gtccgttccg
     3841 aacaggaatt tagcgaaggt catttgcaca acgcggtcaa catccccgtc gaccaaatcg
     3901 tccgccgcat acacgaagcc gcgcccgaca aagacacgcc ggtcaacctc tactgccgca
     3961 gcggacggcg tgccaaagcc gcccttcagg agctgaaaaa agcaggttat acaaatgttg
     4021 ccaatcacgg cggttatgaa gacctgctca aaaaagggat gaaatgacgc aatgccgtct
     4081 gaaaggacag gctttcagac ggcatacgac accccagcac gcggcgcaac atcgggcgcg
     4141 gcgcgtgccg ccccgtccga agcctgataa acaaaccgaa aaggaaaaag gaaatacgat
     4201 gaaacacata ctccccctga ttgccgcatc cgcactctgc atttcaaccg cttcggcaca
     4261 tcctgccagc gaaccgcaaa cccaaaacga aaccgctatg accacgcata ccctcacctc
     4321 aaaatacagt tttgacgaaa ccgtcagccg ccttgaaacc gccataaaaa gcaaagggat
     4381 ggacattttt gccgtcatcg accatcagga agccgcccac cgaaacggct taacgatgca
     4441 gccggcaaaa gtcatcgtct tcggcacgcc caaagccggc acgccgctga tggtcaaaga
     4501 ccccgccttc gccctgcaac tgcccctgcg cgtcctcgtt accgaaacag acggcaaagt
     4561 acgcgccgcc tataccgata cgcgcgccct catcgccggc agccgcatcg gttttgacga
     4621 agtggcaaac actttggcaa acgccgaaaa actgatacaa aaaaccgtag gcgaataagc
     4681 ccgtcgccga catatggcgg atggaaatca aacacacacg cccctcattc ccacgaagtc
     4741 gggaatccaa gcggttgggt taccgtgatt ttcaatcatt gccgaaacgg acgattccgg
     4801 attcccgccc ttgcggggat gacgggcgtg tgcgttttgc catatcacag gcaactttgc
     4861 cgcaaatctt atctgtcgcc ggtatgcctt cttgtttcaa tccgccacaa accaaatgcc
     4921 gtctgaaacc tgatttcaga cggcatttcc gtttattccc tgctgtcacg gcaaatatcg
     4981 atggcttcct gcaaactcga aacgccgtag attttcaggt tcggaaactc tttggcgttg
     5041 cgcggcatat tggctttggg aacgatggcg cgtttgaagc cgagtttttc cgcttctttg
     5101 agccgctctt gcccgcgtgc gacggggcgg acttcgccgc ttaagccgat ttcgccgaaa
     5161 accacggttt tttcaggcat agggcggttg cggaagctgg aaagcatcgc gaggatgacc
     5221 gccaaatccg ccgccggttc gccgattttc acgccgccga cggcgttgag gaacacatcc
     5281 tgatcgaaac aggcgatgcc gccgtggcgg tttaacacgg caagcagcat cgcaagacgg
     5341 ttttgttcca gtccgacggt gaggcgtttg ggcgtgaagc cgtgcgcgtc atcgaccaat
     5401 gcctgaattt cgaccaaaag cgggcggctg ccttcctgtg taaccaaaac gcacgagccg
     5461 ggcgtatcgt cgcggtagct ggcgaggaag atggcggacg ggttggacac acctttcaaa
     5521 ccgttttccg tcatcgcgaa cacgcccagt tcgtttgccg cgccgaagcg gtttttgatg
     5581 gcgcgtatca tgcggtagtt ggaatgttgg tcgccctcga aatacagcac ggtatcaacc
     5641 atgtgttcca gcacgcgcgg gccggcaatc gcgccgtctt tggtcacgtg tccgaccagt
     5701 atcatggcga tgcccatctg tttcgccatg cgcgtcagtt gggcggcaca ctcgcgcacc
     5761 tgcgacacgg agccgggggc ggacgtgatt tggtcggaat acatggtttg gatagagtcg
     5821 atgacgacaa cttcgggctg atgctgtttc aaggccgcct gaatcgcttc catgcggatt
     5881 tcggcaagaa ggtttacgcc gtcggtcggc agttccaaac gctgcgcgcg cagggcgact
     5941 tgttgggcgg attcttcgcc ggaaacgtat agcactttac ggctttgcgc cattttggcg
     6001 atggtttgca acagcagcgt ggatttgccg atgccagggt cgccgccgag caggatgacc
     6061 gcgccatcga ccaaaccgcc gcccaatacg cggtcgagtt cgcccatgcc ggtcgggttg
     6121 cgcggcactt cggtcgcggt aacggcggag agggattgga cggtcgaggt atccgccgcc
     6181 caagattgga aacgggcgtt tttcggctcg ggcgcggcaa ggctttcctg aagcgtgttc
     6241 cactcgccgc aatgcgggca tttaccctgc catttcgggg aggtgccgcc gcattcggtg
     6301 cattggtaaa gggttttaag cgtttttgcc atcggttttc cctgcatctg aaaacaaaaa
     6361 tgccgtctga aaatagggac ggttgggatt acgccaatac ggcaacaccg ccgccgtcat
     6421 gtactatccg tacacggcgg atattgccgt attacctaat tttccatccc tcggctttca
     6481 gacggcatat cgggcggttt cctgccggct tacccgccgt gatactgccg cgacagctcg
     6541 tgtacggtat cgactaagat tttggcgtgt tcggggtcgg cgtgttggtt gatgccgtgt
     6601 ccgaggttga agacatggcc gctgccgtgt ccgtagtcgg ctaggatacg tgcggcttcg
     6661 gtgcggatgg attccggcgt accgaagagg gcgaacgggt cgaagttacc ttgcagggcg
     6721 acttgcttgc cgacgcggcg gcgtgcttcg ccgatgttgc acgtccagtc caagcccaat
     6781 gcgtctgcgc cgatttctgc catgctttcc agccacagcc cgccgccttt ggcaaacacg
     6841 ataacaggca cgcgcctgcc ttcgctttcg cgtttgagtc cggcaacaat ttggcggatg
     6901 tatttgaggc tgaattcttt aaacgccgcg tcgctcaaca cgccgcccca agtgtcgaaa
     6961 atctgcaccg cctgcgcgcc cgcgtcgatt tgggcgttga ggtaggcggt aacggcttgg
     7021 gcgttggtat cgaggatttt gtgcagcaaa tcagggcgcg agtacatcat ggttttgatg
     7081 gtgcggaatt ctttgctgcc gccgccttcg accatataac aggcgagcgt gaacgggctg
     7141 ccggagaagc cgataagcgg tacgcggccg tccagtgctt tacggatgga gctgacggca
     7201 tcaaagacgt attgcagttt ttccatatcg gggacgtgca atttggcaat gtcgtcctcg
     7261 tgttgcaggg cgcgtttgaa tttcgggcct tcgccttcgg caaaatacag tcccaagccc
     7321 attgcgtcgg ggacggtcag gatgtcggaa aacagaatcg ccgcgtccaa atcgaagcgt
     7381 tctaagggct ggatggtaac ttcggtcgcc aattcggtgt ttttacacaa atcgaggaag
     7441 ctgcccgctt tcgcgcgtgt ggctttgtat tcgggcagat aacgccccgc ctgacgcatc
     7501 atccaaatcg gcgtgtattc gacgggctgt ttaagcaggg cgcggaggaa ggtatcgttt
     7561 tttaaaagag tcatacgggt tttctgtcag tctgtttgtc aagtgaaatg gttttcagac
     7621 ggcatttcaa cgatgccgtc tgaaggtttc aatgggtttc ggcggaaggt cggttttcct
     7681 cggcaacgct tgccaaaacc aagttgcgct gcgcctccgc cttctgcggt tctccggttt
     7741 cgtcaaaaac ctttgccaga accaaacgcg cggaaatact cggctttaat gcaatgctcg
     7801 cttcaaggta gccttttgcc ttgccccaaa gtttgcggcc gtaggcgagc cgaccgagat
     7861 acatcagcag aagcgcatta tcgggctgtt ctttcagcca agcatcggca aaatcgatgg
     7921 ctttctgctg atcgcgttcg cccaaaaagc gcacgctttc gacaaaggct tccaaaagtt
     7981 cgggtcggcg gttgtgcgga taatgctgtt tgacccattt gaccgcatcg gcatacagtc
     8041 ccaaacgttc gtacttttcc gcaaccgata cgctcaattc cccgtttttg aggctgtcgg
     8101 gaatccgctt caggcaggtt ttcaaagcgg cggcatcggc agcatccgcc agctggcggc
     8161 ggtatgccca attttgatac cgttccattt ccgatttgcc caacgcgccc gccttggaaa
     8221 gtttttcggt ttttgccaga acctgcaacg cgtcgcccct gtcgaaagcg taacgaagtt
     8281 gcagacgcac gaggcgcgta aggttggcat tcatcttcgc cgccgcataa agattggctt
     8341 ccgccgcttc gtaatcgcgc cggttcaacg ccgattccgc caacaaaaga taacgggaaa
     8401 gctgctgttt ttccggcagt ttggcgattt ccgcaagata acggtcgcgc agctcgatgt
     8461 tttccatctg tccggcggcg tgcgcaccca gcatcaatgc caaagtccgg ttgtcgcccg
     8521 cctctttgtt gcccaacacg cgcgaggctt caagttccgc cttttcaaaa cgcccttcaa
     8581 aatacgccaa acccgccttg ttcaaggcaa gcgcggcctt gcggccttta cgcgccgaac
     8641 cgaaacgctg catcttttcg gggatattga atacgccgat aatgaattta aacaggaaat
     8701 accacaccac gacggcaatc agcgaaccta acacaaaagc gtgcaggttg attctgagca
     8761 tggtctgtcc gagtacgata tacacgtcgc cggtgtaaat gcccgaagcc agcgccagtc
     8821 cgacggcggc ggcaaacagg acgacaatcc agactaccgt tttcatgcgc gttctccttt
     8881 agcggacggc gaacgccctg ccttttccgg tacgggctgt ttttttgcag gctgttccgg
     8941 tttgcgttcc gaaggcaggg acggtgcttc tatggtcttc acatccgatg cggcagccgg
     9001 ttcggaagct gtgttttcgt ttgccggttc ggaagccgcc tgttctgctt cttgattttc
     9061 cgccgccgtc atgcgcgtac cgtcgcgata ggcgcggacg gcatttaggc tgtttttcaa
     9121 accatcatcc gcagtcatgc gcacatccaa cgccttcaat tccgccagtt ctttcagcca
     9181 cgactgcgtg gcgggagact tggcatcgaa atactgtctg acggcggctt cggcattgtt
     9241 caaatcgccc tgatagactt cgctgttgcg ctgcattaat gcagtgcgca catccaaaag
     9301 gcgaaggcgc aggttttcac gcacaaaata tgcctgttcg ggagaaatca gcatggcatc
     9361 gttgttttcc aaacggcgga tttcgaccag ccccttcaat gtgccgaggg atttttccca
     9421 tacgttctgc caccatgaag cggaagcggc ttcgttcttc acctgtacgc ccggtttcaa
     9481 tacgccgtcc aaaatcagcg gcagtccgaa tacggcggtt tccagcctgt cgaggcgcaa
     9541 tgccgtgccg gaaatatcga catagggacg gtttttcagt tccgccaagt cgctgctgac
     9601 tgcctgtttg atcggcagaa gctctgcctg atcgaaacgg gacaggcggc tgtcgatatg
     9661 ctccaacacg ccgactgccg tttggatatt gccggtcaac accagctgtt gcgctgccaa
     9721 attgagtatg gtatcggttt cgtccaccag ccaatcggca cgccctttag tcagctcgcg
     9781 ataggctttt tgcgtcatca gaatctgttc gccgttggct ttgacaccgc tgtccaaacg
     9841 gtcgagctct gattgtatgg cggcttgccg gttgaggttg tctttcaaca gggcggcgtt
     9901 ttccgactcg cccaaagcgg ctttgtcgat tttttggttg aatgccagct cttggttttt
     9961 caagacattc tgtccttgga caaacaaaaa accacttgta cccaaaccca atgccgccaa
    10021 taccagggcg cagactgcca aagcgttgct gccggactgt ttgacgataa aaggagcggc
    10081 aggaatgtgt acttctgttt cgttttcttt gcgtggggaa gaggtttcag acggcatttc
    10141 ttttgatgcc tgtatctcgc gtacgggttc ggatgatttg ttttcaggtt cgcccaccgt
    10201 tttgctcctt attaatttga ggttccggga aagaccatgc cgtctgaaac ggaaatggaa
    10261 gaatgggaaa gcgcggcttc cagcgtaggg acggtttcga ccgaacacac gccttcgcgc
    10321 ttcaatgcct ccgcaatgcg cggatgatgg gtaaagtata gcaaggattt gaagaatcgg
    10381 gaaaattgcg gcggaagctg tccgaacagc aagcgcacga gttctgtgga cgtaatatag
    10441 gcggcggcaa tattttcggt ttggaaattt tgaaagttca aaggtttatg ccgtctgaaa
    10501 tagacttctg ccacctccgt ccgaaaacct ttctcctgca aggcattcat cagaaaatcc
    10561 cgcccgccgt gtccgcgcac aaacaatacg cgcgcacctt cgggcagact gttccaaacc
    10621 gtcaggcgca aaaccgcctc gctgtcgttg ccgtcttcgg gcgcgatgac cgttctgccc
    10681 aaacagcgtt ccaatgcacg gcggctgccc tgccctacgg aaatgtgcat ctttatgccg
    10741 tctgaaaggt taaggtacgg gacggcggtt tcaacggcgg tcggactgac ccaaaacacg
    10801 gcatccgcac gggcatattg ttcggacaaa agttccagtc cggcagcatc tgcttcgatt
    10861 tcaatcgggc tcaacacttc cgcctgccat ccggcacgac ggcaggtttc cacatcgtcc
    10921 ttcgccctac ccgacggtcg gacaatcagc atgatgggtt tcactgtttc catcgcctta
    10981 tccgccccga ttaccgggaa aaatcagcat cgttgaaatt cgcctgcgcg gtttgcggcg
    11041 tacagccgac ccgtgcgact tccttgcccc tacgctccac aacaacaccg ctgctttccg
    11101 tatgagtcac ggaatcgaag cccgtccata cggtgtacat aaattcgccg ttttggaatt
    11161 tcatcgttgc ccggcgtccg ctgcccatgc cttgccacct gtcggaacgc cccaacaggt
    11221 cagctttgct gttgcgtatg gcaatttctt tttttgccgc actgccgaac gaatattcgt
    11281 aaagattgcg gttgattttt tgaacttcta tatatttgtt gttgtccgtt ttacaggaaa
    11341 acaccgtatc cgcccacacg gttcgggtcg cagccgacag aagcaataca gcagaaagga
    11401 acattttttt catcgtattt ccttaatggt tgaaactccg ccatttggac atccgtcctt
    11461 cgtggcagca gcatcagatt ttatttgaga ggggtgtaac cccttccaaa ttagggcaac
    11521 acatagggca acgctttatg tgtcgtcctg cgtgttgaaa catcattgcc cgtatcctga
    11581 aatagaagtt ttacggtctt ctttccgaca aaatgccgtc tgaaacggat ttcagacggc
    11641 atggtcgaaa attattgcgc ggcaagcggc ttgagcagca aggtttcgcc gctttgcaca
    11701 actttttcaa gcaggtcgat attgggatgc ttgccgggat gggcgcgcgc gtcggcaacg
    11761 tgttcggcaa accatatcaa accctgttct gccgcttctt tgtccaattt tccgtcaaaa
    11821 cgttctgcca aagcgttgta caacttgagc gaaccgagct taccctgaac ggcaggaatg
    11881 tggtgaaccg ttttgccatc tgcatcctga acgtccaaac cgcccagatg gtcgattgaa
    11941 gaaaatgtgg cgagatagtc ttgaaaattc atatcaaatc cgaaaatcag tcagttagcg
    12001 cgtgccgaaa atcttgtcgc ccgcatcgcc caagccgggg atgatgtagc cgttctcgtt
    12061 caagtggctg tcgagcgcgg cggtgtaaat cgtaacgtcg ggatgcgcgt cgttgaccgc
    12121 cttcacaccc tcgggcgcgg caaccagcac cagtgccttg atgtttttgc agcccttggc
    12181 tttcaaaagg tcgatggtgg caaccatcga accgcccgtc gccagcatcg ggtcgataat
    12241 cagggcagga cgttcgtcca tgctgtccac gaatttctcg aagtaggaaa cgggtttcag
    12301 cgtttcctcg tcgcgctgca gtccgactac gctgattttg gcagtcggaa tcaggtcgag
    12361 tacgccgtca agcataccca aacctgcacg caggatggga acgacggtca atgttttgcc
    12421 cttgatgcga tcgccttcaa tctgaccgca ccatccgtcg ataaggtatt tttcgatttc
    12481 aaaatcacgg cttgcctcgt atgccatcag gcgcgccagc tcgatggcaa gcgtccggaa
    12541 tttgtaggtg ctgcaatccg cctccctcat cagggttaat ttgtggcgga cgagcggatg
    12601 gttgataaca ttaacgttca tcgcggtgtt ccagtgtgat tttgagacgc gccattatac
    12661 ttttttttga tttgccggaa aagaaaatgc cgtctgaaac acgttcagac ggcattgccg
    12721 aatccatcag gctttcagca gctcttgcag ctcgcctgcc tcatacattt ccatcaggat
    12781 gtccgaaccg ccgacaaact cgccgttcac ataaagttgg gggatggtcg gccagtcgct
    12841 gtattcctta atgccttggc gtacttcggg attttccaat acgttgacgg taacgtaatc
    12901 ggtgcagcct gccgcgttca ggatttgcac ggcgcgggaa gagaaaccgc attgcggaaa
    12961 ctgcttcgta cctttcataa acaatacgac gcggtgtgtc gttactactt ctttaatttg
    13021 gtcgtggatg gaagccatcg cttattcctt ttgataaagt tgacaaaatt tgtcaggtag
    13081 gacgcattat acgcaaaagc ggaaacttcc cacaatattt gccgtatatc gcgcagtatc
    13141 aatgttgtga acggaaatat tcccactcct cgacaatcct actgtccggc aaatggcaca
    13201 gggcatatcc gccgccgctt tcgtcttttt ttacttcccg tctgccgcct ttggcaatgc
    13261 agaatttgga agcggaattt gccatgcctg tccgaaacgt gttggcaggc agccggcagc
    13321 agcgcggaca tgcatacgga tgaattgcct ccatggtttt ccttatcgtc ggtacagggt
    13381 cagacggtcc gcatatcggg tactttttta aacagccggt aaaagttttc cgtcgtgtat
    13441 gccgcaacct gttccaatgt ttgattccgc aatttggcga tatgttcggc ggtatggcgc
    13501 acaaaagccg gttcgttctg cctgcctcgt ttgggaacgg gggcgaggaa cggcgcatcg
    13561 gtttccacca aaatgcggtc gtccggcaca tattttgccg cctcctgaac caagggtgcg
    13621 tttttaaagg taacgattcc cgagaaagaa atataaagcc ccaaatccat tgctgcacgg
    13681 gcaaaaccga tgtcttcgga aaaacagtgg ataacgcccg aattaacccg gcattctttc
    13741 aggatagaca aggtgtccgc cgccgcatca cgcgtatgga cgataacggg cagtccggtt
    13801 tgattggctg cttcgatgtg gtctgcaaag cgtttgtgtt gccaggacaa atcgcctttg
    13861 caccagtaat aatccaaacc cgtctcgcca atgccgacca ctttcggcga ggcggctgcg
    13921 gcaaccattt ccgcaataga aaattcttcg gcttccttgc tgtcgggatg tacgcctatt
    13981 gtgcagtaaa tatgttcata aacttcggcg atagcggata cttcggcaaa actttccctg
    14041 ctgacgctga tggcgagcgc ctgccccacg ccgtttgctt ccatgttgga caaaacttcg
    14101 ggcaggcgtt ctttcaaacc ttcaaaattg aggtggcagt gcgaatcgat gatatgcatg
    14161 gcgttacatg gtaaacgtag gtctgctgcc gccgattgtg ttgcccagtt cgacttcgat
    14221 ctggtctttg actttcaggc agttttcgtg ttttgtgaat gccagcccca cccctttggg
    14281 tttggaggaa gtacgcgcag gattgatcca ggcgactttg gtcggcagga acagtttggg
    14341 gaagttgagg atttctacgg caagcagaat atcgtccccg atggaaaata cgtcgttggt
    14401 ctgcacaaac agaccgccgt gttccaaaaa cggcatgtag gagctgtaca gcagattcat
    14461 gtctttcagc tgcaacgaca tcatttttgc cggaatattt tgtccgtctg acataattca
    14521 cccgttctct ttcttcaatt ccaaatagtt gatgagcaga tgctcgatct gcattttgac
    14581 atttaaagta tgaaacccgt agggggcaag ctgtttgagc atatcctccg ccgcaaatac
    14641 attgcgcgga cggaaaccgg ataccgtctg aagcagcctg tcttcataag cgggataata
    14701 gacgggtttc atgtgttgca ggcacaatcc caaatcgacc agccatttct gcatccaccc
    14761 gacaaatacg gcgagcggaa gtttttcctt atcgaaaagc gcggcgtaat ccaaaatctt
    14821 caacaacctt ggttctgcca aaatatccaa cagtttaatc cgcaacgcac ggacaccgtc
    14881 cgcctcatca aacagcggcg ctccggaatg gaaagccaga cgttcctcag gttccgccac
    14941 acccctttca cgcagatatg ccaatgcctc ttcatgggaa ggcgcgggca aaaccatctt
    15001 ccggcagcgg cttttaatgg tcggtaaaac cttgtccgcc gcgtggctga ccagcaaaaa
    15061 gaccacttgt ggcggcggtt cttccaacac tttcaacaaa ctgttggcgg cttggacatt
    15121 catactttcc gcaggatgaa tcaggatcac gcgcaaaccg ccccgtaccg aagtcaggta
    15181 cacattatcg atgatttccc tgacggcatc gattttgatc tgcaacagtt tgcgtccgtt
    15241 ttcgggttcg tccgacaagg gggtgatttc gtaaaaatcg ggatggcttc cctgtccaaa
    15301 cagatggcag gacatacatt cgccacaggg tttgcagccc ggtgcagggg tttcgcacaa
    15361 cagtgccttc gccgcaaagc gggcaaatgt agttttcccc gtatcttttt tgccggcaaa
    15421 cagccatgca ttgggacgac gctcccaatg ttccgcaatc tgccgccatt gctcattatg
    15481 ccacggataa atcatttcaa accatccgtc cggaaatcca taccgccatc agagcagttc
    15541 caaatcgggg ctggaacttt gcgcgcggcg gctgcgcaac tgtaccgcca aacgcaaatc
    15601 gtgtgcggaa tcggcatttt tcaatgcttc ttgcagggaa atatcgcctt tttcatacaa
    15661 ttggtaaagg tgttgatcga aggtctgcat acccagggta gtggattttt tcatcacttc
    15721 tttgatttca tggatgttgc cgttgtgaat caactccgaa atcaggggcg aattgagcag
    15781 cacctcgact gccgccaccc tgcccttgcc gccgtctcgc ggaacgaggc gttgcgaaat
    15841 aaacgcctga aggttgagcg acaaatccgt cagcaattgt tcgcgccgct cctcggggaa
    15901 aaagttgatg atgcggtcga gtgcctgatt ggtgctgttg gcgtgcagcg tcgccataca
    15961 caaatgcccc gtttcggcaa aggcgatggc gtagtccatt gtttcgcggt cgcggatttc
    16021 gccgataagg atgacatccg gcgcctgacg cagcgtgttt ttcaacgccg ccatccagtt
    16081 ttccgtatcc acgccgacct cgcgctgggt gatgatgcag tttttgtgtt cgtggacaaa
    16141 ctcgatcggg tcttcgatgg tgatgatgtg tccgaacgaa ttttcattgc ggtaatcgat
    16201 aagcgaggca agcgaagtcg atttgcccga gccggtgccg ccgacaaaaa taaccagccc
    16261 gcgttttttc agcgcgacat ccttcaagac cggcggcagg ttgaggcttt caaacttggg
    16321 aatcttgctg gtaatcgcac ggaataccaa cgccgtcgcg ccgcgctgta tcatcgcatt
    16381 gacgcggaag cggctggtgt ccggcaggct gatggcgaag ttgcactcgt tggtcgatga
    16441 aaattcttcc gcctgcttcg cactcataat cgaaaaggcg atttccatac atttttccgc
    16501 cgtcagcggc tcgtccgtga tgcgggtgat tttgccgtcc aacttcatcg cgggcgggaa
    16561 atgggtcgtc acgaacaggt cggaaccttt gttttggttc atatggcgca gccatgcgaa
    16621 cagttcttcc tttgcggtca tattttcatc ggtaaacatc ctcgtcccct aatcttcaaa
    16681 caatctgtac cttcccattc taccaaaaac ccgccgtgcc gtctgaaacc gtttcagacg
    16741 gcacggacaa accacctcca ctcaatcctg ccgtgttagc gtgtataatc aggcattcta
    16801 tccgaaattc acaacaggtc agcaatatgt ctttgaaaac agtagccgtt atcggcgcaa
    16861 tggaacaaga aatcgagctt ttgcgcgaga tgatggaaaa tgtcaaagcc gtctctttcg
    16921 gcaagtttac cgcctatgaa ggcgaattag cgggaaaacg catggtgctt gcattgagcg
    16981 gcatcggcaa ggtcaacgcg gcggttgcaa cggcttggat tatccgtgaa ttcgcagcgg
    17041 actgcgtcat caacaccggc agcgcgggcg gtttgggcaa gggtttgaaa gtcggcgacg
    17101 tggtgattgg cacggaaacc gcacaccacg atgtcgatgt aaccgcattc ggctatgttt
    17161 ggggacaagt gccgcaactg cccgccgtat tcgtttcaga cgacctcttg gtcggcaagg
    17221 caaaacgggc ggcgcggacg tttgaaggcg cggcggtaga acaaggtctg attgtcagcg
    17281 gcgaccgctt cgtccacagc agcgaaggcg tggcggaaat ccgcaagcac ttccccgaag
    17341 ttaaggcggt agaaatggaa gcggcggcaa tcgcccaaac ctgtcatcag ttggaaacgc
    17401 ctttcgtcat catccgcgcg gtttccgatt cggcagatga aaaagcagac atcagctttg
    17461 acgaattttt gaaaacggcg gcggcaagtt ctgcaaaaat ggtggcagaa atcgtcaaat
    17521 ctttataaaa cctttatcaa atcatgccaa aacagcggtt ttccgttaga atatcgggct
    17581 atttccacgt tgcgcccgat attcggacgc aacgccgttt gttcagacgg catttcccaa
    17641 acccactgtc cgacattcaa aacaactcta atgaaaaata tccgaaattt ctccatcatt
    17701 gcccacatcg accacggcaa atcgacgctt gccgaccgct tcatccaata ttgcggcggt
    17761 ttggatttgc gcgaaatgag tacgcaggta ctcgattcca tggacatcga aaaagagcgc
    17821 ggcattacca ttaaagcgca aaccgccgcg ctcaactata aagcacgcga cgggcaggtg
    17881 tatcaactca acctgattga cacgccggga cacgtcgact tctcttacga agtctctcgt
    17941 tcgctctctg cctgcgaagg cgcgcttttg gtcgttgacg cgtcgcaggg cgtggaagcg
    18001 caaactgtgg cgaactgcta taccgcgatt gatttgggcg tggaggttgt gcctgtttta
    18061 aacaaaatcg acctgcccgc cgccgacccc gaacgcgtgg aacaagaaat cgaagacatt
    18121 atcggcatcg atgccgtcgg tgcggtgcaa tgttccgcca aaagcggcat cggcgtggaa
    18181 gacgttttag aagaaatcgt tgccaaaatc cctgcgccga ccggcgacga aaacgcgccg
    18241 ctgcaagccg tgatcgtcga ttcgtggttt gacaactatg tcggcgtggt catgctgatt
    18301 cgagtgaaaa acggcaccat caaactgaaa gacaaagtgc gctttatgag caccaaggcg
    18361 gaaacgcagg tcgagcagct gggcgtattc acaccgaaat cggttcagaa acaagaactc
    18421 aaagccggcg aagtgggctt tttgattact ggcgtaaaag aattgggtca ggcgaaagtt
    18481 ggcgatacgg ttacactggt tgccaaccct gccactgagc cgctgcccgg cttccaagaa
    18541 gtacaaagcc aagtattcgc cggactttat cccgtggaaa gccacgacta cgaagccttg
    18601 cgcgacgctt tggaaaaatt gcagcttaac gacgcttcgt tgaaattcga gcctgaagtt
    18661 tcccaggcat tgggcttcgg cttccgttgc ggcttcttgg gtctgctgca cttggaaatc
    18721 gtgcaggaac gtttggagcg cgagttcgac atggacttga ttaccaccgc gccgacggtg
    18781 gtttacgaag tcgtgttgaa aaacggtgag aaaatcgaag ttgaaaaccc gtccaaactg
    18841 cccgacatcg gcagcatcga aaccattctc gagccgatta ttaccgcgac catcctcgtg
    18901 ccgcaggaat acgtcggcaa cgtcatgact ttgtgtaacc aaaagcgcgg cgttcaggtc
    18961 aatatgcaat atatgggccg ccaagtcatg ctgacttatg atttgccgat gaacgaagtc
    19021 gtcatggact tcttcgacaa actcaaatcc acttcgcgcg gttatgcttc gttggactac
    19081 catttcaaag agttccaacc gtctgatttg attaagctgg atattatggt caacggcgaa
    19141 aaagtcgatg ccttaagcct gattgtgcac cgtcaaagcg cggttcaccg aggccgcgag
    19201 ctggcatcga aaatgcgcga gctgattccg cgccaaatgt tcgacattgc cgtccaagcc
    19261 gccatcggca gccagattat cgcccgcgaa aacgtcaaag cactgcgtaa aaacgtcttg
    19321 gcgaaatgtt acggcggcga tattacgcgt aagaaaaaac tgcttgaaaa acaaaaggca
    19381 ggtaaacgcc gtatgaaaca agtgggcaat gtggaaattc cgcaaagcgc gttcttggcg
    19441 attttgcagg tgagcgacaa ataactctgg cacacagggc ggcgcgtcgg gaacacccct
    19501 caaaccgccc tgatttcagg ggcacgcgcc cgcaaacaaa ttttgcaatt tgccgcctta
    19561 aaacagttgc acggccgggc gggtttttaa ccgttgagga acaacaatga acacaatgct
    19621 aatgtcgggc gcggctgccg cgctgcttgc cggcatcatc ctttatttca aaagcgacaa
    19681 gaagcggcag gaaaacgggg aatggagttc cggccttgaa tacgcctata tcctgacagc
    19741 ggtcggcgtg tttgccgctt tgtccctgtt tatgagcttt accgccgttt tcctgatttt
    19801 cgttgtattg tgcggtacgg cttggggggt atataaatac cgcctgaaga ctcatcccga
    19861 aatctcggaa agcagccact tcggcgatta tttcggcagt ttcttcccta ccgttttggt
    19921 attgttcctc atccggtcgt ttatcgccga accgttccaa atcccgtcca gctcgatgcg
    19981 cccgggcctg atcaagggcg atttcatttt ggtcggcaaa ttttcctacg gcctgcgcgt
    20041 acccgtttta aacaatgtat tcatccccac gggcaaaatc gaacggggcg atgtggtcgt
    20101 ttttaattat cctttgcaac cggagatgac ctacatcaag cgtattgtcg gcattccggg
    20161 cgatgtggtc gaatatcgga ataaagtttt gacggtaaac ggaaaacctg cttccgatat
    20221 tcctgacggc acataccgtt atcccgacga caccgaccct tccgaaatcc acaatacgga
    20281 tatgttccgc agcggtttgg acggcaaatc cttcaatatt ctgaaaaaag aaggacagcc
    20341 tgccgtttcc ctgcccgtat tgggcaaata tacctccgat attatgtctg aaaacggata
    20401 ttccatagag caaagcggtt tggaacactg ccaatatgcc gacgacggca gcggtttcgt
    20461 gtgcaaagtt cccgaaggac gctatttcgc tatgggcgac aaccgcgaca acagtgccga
    20521 ttcgcgctac tggggatttg tggatgacaa gctggttgtc ggcaaggcaa tgttcatttt
    20581 gatgaacttc ggcgatttcg gcagggccgg tacggcaatc cgttaggccg gaaaattacg
    20641 ccggaacatc aaaaatgcca tccgaaccct gtttcagacg gcatttttat catcaaacgc
    20701 cttgtttccc tgccggcagc ctcggggata atatagtgga ttaaatttaa tccactatat
    20761 ttccgtttgt gtggaaaccc atcccattga ataaagggca tttcgtccga ctgacggtgt
    20821 gggcggcata tggaaatacg gacattattt tagttgcatt gccattccag cggaggcggc
    20881 ttttttgatt ctgcccgccc cgatgccgtc tgaaagggct tcagacggca ttttccgctc
    20941 cgcgccgttg ccggcgtgtt tggtcttgcc ggttttgact gcctgccagt tcaatcgtac
    21001 gcaggtttgc cacggcagaa aatctgatgc cggacggagt caaggctcgg actgctgttt
    21061 cagcgaaccg agataaattg ccgaaagcgt cagcgccacc cctgcccact gcacgccttc
    21121 aatcggtttg cccaatccga aataatcgat gaacagggcg gcaaccggtt cggacaaaag
    21181 cagcagcccc gtcagcgaca gcgaaagcag cggaatcgca taggcaacca tcgcccacgc
    21241 gaagcactgc atcaccacgc cgtacacaag caccaaaccc gcatctttcc aagtcgtcgg
    21301 ataaagcgcg ccgccgtcca tcagcaatgc cggaacaacc aacgataccg cgccgcctaa
    21361 actcaaaatc atcattgaag ggaaaagcgc caccggctcg acttcatgag tcttgcggac
    21421 gaacaccatc gacagggcaa gcatcagccc ggacaccaaa ccgctgacga atccccaaac
    21481 cgcattaccg ttgtagccga attccgcacc ggcaatcatc gccacaccgg caactgccga
    21541 tattaagcct gcctttttca gcccgctcaa acgctcgccg aaaaagaaaa caccgattgc
    21601 cgacaagaaa aagatttgca ggctgttgag cagggtggaa atacccggcc cgaccgcgtg
    21661 tatgctttcg tgccacaacg ccaaatcgaa agcgagaaac acgcccgccg tcagggcata
    21721 tcggacggtt ttcctgtttt ttgggaattt ttgcccgaaa aaccgtgcta aaaaccaaaa
    21781 tacgaacacc gaaatcagca accgccaaaa tgcgattgca tacgaaccga cggggacgga
    21841 tctgacaatc agactgccca gaccgaacac cacgcaaccc agcaccagca atggtgcggc
    21901 atattttttg ccgttaccga ccacaatatc ccctcccttc cccatacggc tgccgcgccg
    21961 acggcaagaa tgccgccatt ccgaaaatat gccgtctgaa gcccaaagcc ccggctgcac
    22021 ccggacaaac ggctgatttt acgggcaggc cgactatttt ccgttggggc aaaaaaccgt
    22081 cctgccagat aaaacccgaa tatagtggat taacaaaaac cagtacagcg ttgcctcgcc
    22141 ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact
    22201 atagtggatt aacaaaaacc agtacagcgt tgcctcgcct tgccgtacta tctgtactgt
    22261 ctgcggcttc attgccttgt cctgattttt gttaatccac tatatctgta ctgtctgcgg
    22321 cttcgttgcc ttgtcctgat ttttgttaat ccactataaa aaatatttcc ctcaagcaat
    22381 gcggcggcac caatgcctga ttaaaacaca accggcgttt tcggcatata tgcaaaaacg
    22441 cattttccgg cacggcgtta caccgatacc gaaaaatgcg tctgaatgtt tcgacaatcc
    22501 gattaaagca ttatgccaaa tctgcaaaaa gtggcgtaga gagatagcgt tcgccataag
    22561 aaggcagcag cacgactatc agcttgcctt cgttttcagg ctgtttggca agctgcaacg
    22621 cactccaaac cgccgcaccg gaagaaatac ccaccaaaat gccttctttt tccgccattg
    22681 cgcgggcggt ttcaaaagcc gcttcgttcg acactttggt aatgctgtcg taaattttgg
    22741 tattcaaaac ggtcggaata aagcctgcgc cgatgccttg aatcgggtgc gggccttttt
    22801 cgccgccgct caatacaggt gaagcttcag gctcgacggc aaccacttta acttcgggtt
    22861 tgtatttttt caacacttcg cccacgccgg taatcgtacc gcccgtgccg acgccggcaa
    22921 cgaagacatc gactttgccg tccgtatccc gccaaatttc ctcggcggtt gttttgcggt
    22981 ggacttcggg gtttgcctca ttgtcgaact ggcgcggcat aaaataagtg tcgggatgcg
    23041 cgtccaccaa ggatttcgct ttggcaatcg cgcccgccat accttcggcg gcaggggtca
    23101 gaatcagctc cgcaccaaac gcgcgcaaca gcattttgcg ctccttactc atgctttccg
    23161 gcatggtaat cgccagcttg tagccgcgtg cggcacatac cattgccaaa ccgacacccg
    23221 tattgccgct ggttgcttcg acaatgacgg tgtttttgtt gattttgccc gctttttcgg
    23281 caccctcgat cattgcttcg gcaatgcggt ctttgacgct gctgcctgga ttgaaaaatt
    23341 ccagtttcac ggcaacctcc gccttcaaac cttcggtcag acggttcagt ttgaccaaag
    23401 gcgtgttgcc gattagttcg gtgatgctgt ttgcaatttt catcttctac tcctgaataa
    23461 taaggtttcg acagtaaggc gcaatcccca taggcatcct gtccgattgc aggggatttg
    23521 cgccgaaaca taaacaaatt tcggatatgg ctttatccta aacctatgcc gcctaaaaaa
    23581 ccaatatcca aaatttcttt ctttataact ttttgttccg atacggccgc accgtattat
    23641 cttccaattt ctgaaagcag cagcaccagt accgagacct gcatcaacgc tgccgtcaac
    23701 ccgccgagtc gcagctgcga gccgccgaac gttgacggcg gctgccggtc tataccgcag
    23761 cggtggacat aggcaaccgc catcagcaca aactgcaaca cgaaccaata aaccggcttc
    23821 agcgcataaa gtttgagcag tgccgcaaac acaaacactt gaaccgttcc cgaaaagcaa
    23881 taatgcacca acgcacatat tccgacaaaa gcaagcgtca tcgatacatt actgacggca
    23941 aacagcccga gcagaggatg ttcggggtct gcctgccatc cgtttccatc cgttttccgg
    24001 ttccaatgcc cgatgaaaaa aaatatgctg cccaaagtca ggtaaaaatg ggggatgaac
    24061 aagggcgcgg cgcgggtcat tccccatatg ccgggcatca gctttgcccc caaaaccgat
    24121 atgcccagcc acagcataat actcgcccaa aaccattgca acattcctgc gcggaatatc
    24181 agaaacagaa aaaccgccgc atacagtaag gcagacaaaa aatccaaatg cagcaaaatc
    24241 atgaatacgc caactcgcct tcaaacaccg tttccgcagg gccggtcatc atcacatcgc
    24301 cgccgcaggc ccattcgata tataaagttc cgcccggcaa aatcacctct accgttttcc
    24361 cttcatccaa caaccccaga cggatacccg ccaccacagc cgcacacgcg cccgtaccgc
    24421 acgcttgggt ttcgcccacg ccgcgctcga acacgcgcaa acgaatcgcg gttcggctga
    24481 caatctgcat aaagccgacg ttgacgcgtt cgggaaactg cctgtgcggt tcgataagcg
    24541 aaccggtttc gcgcaccggc gcgcattcca cgtcatcgac cacaatcacc gcatgggggt
    24601 tgcccatatt gacgcagctg acaggctgaa tgccggattc gagatgcacc ccgtaaatac
    24661 aggcatcatc cccctcgccc gattcgggga caaacggtat ttcagacggc ataaacttcg
    24721 gtttgcccat attgaccgta accataccgt tatcggacaa tttcggaaaa ataacgccat
    24781 ttgccgtttc aacacaaatt tctttcttat cggtcaaacc cttgtctgca acaaaacggg
    24841 caaaacaacg cgctccgttg ccgcattgcc cgacctcgct gccgtcggca ttgaaaatac
    24901 ggtaacggaa atccacgcct tcggtttccg aacgcccgac caccaaaagc tggtcgaagc
    24961 ccacgccccg gaaacggtcc gcccatgccg caatcggcgc gtcctcgggg gtaaaatcct
    25021 gactgaccgc gtcaatcacc ataaaatcgt tgcccaaacc gtgcattttg gtaaatttca
    25081 aagtgttcat attgttccga aatcccgtat cgtctgttgc ttccgaatcc gaaatccccc
    25141 gcaccgaagg gaaatgcttc catctcccgc cgcctgagga ttaaaatcag ccattatact
    25201 gtttgttacc ccttacaaga aacaccatgt tccgcatatt actgttgctt ttcccttgtg
    25261 caatccttgc cgcctgcacc cgaaacatac cggcaaaccc ggccgtttca aattgcgcgc
    25321 atcttcaaaa ccgcaccatc ctgtattttg acgcacacgg caaacccgcc gacggcgcag
    25381 taaagggcgg attttacaaa attatcgaaa tacagggtgc aaactgcatc cttattcaag
    25441 actttaacag cacgggcaat gccctcggcg gatcctatgt gccggactgc cgcaaatcat
    25501 ccctttcacc gaaaaatatc tgacttcaaa ctgcctatga accacgacat cactttcctc
    25561 accctgttcc tactcggttt cttcggcgga acgcactgca tcggtatgtg cggcggatta
    25621 agcagcgcgt ttgcgctcca actccccccg catatcaacc gcttttggct gatcctgctg
    25681 cttaacacag gacgggtaag cagctatacg gcaatcggcc tgatactcgg attaatcgga
    25741 caggtcggcg tttcactcga ccaaacccgc gtcctgcaga atattttata cacggccgcc
    25801 aacctcctgc tgctcttttt gggcttatac ttgagcggta tttcttcctt ggcggcaaaa
    25861 atcgagaaaa tcggcaaacc gatatggcgg aacctgaacc cgatactcaa ccggctgtta
    25921 cccataaaat ccatacccgc ctgccttgcg gtcggaatat tatggggctg gctgccgtgc
    25981 ggactggttt acagcgcgtc gctttacgcg ctgggaagcg gtagtgcggc aacgggcggg
    26041 ttatatatgc ttgcctttgc actgggtacg ctacccaatc ttttggcaat cggcattttt
    26101 tccctgcaac tgaaaaaaat catgcaaaac cgatatatcc gcctgtgtac gggattatcc
    26161 gtatcattat gggcattatg gaaacttgcc gtcctgtggc tgtaagcccc attcattccc
    26221 gatacccgta aaaaataccg tctgaaacct gttcagacgg cactttcacc ctaagcgtta
    26281 ctcggcggca ttttcctcac gggcaggcgc gtccagcagg gctttgatgg acagacggac
    26341 acggcctctg tcgtccactt ccaatgcttt cacgttcacc acctgaccga ctttcaggta
    26401 gtcgccgaca ttgcgtacgc gctcgtgggc gatttggctg atgtgtacca aaccgtcttt
    26461 gcccggcatc acgctgacaa tcgcgccgac gttgttgtcg aggattttca ccacagtgcc
    26521 ttcatacact ttgcccactt ccacttcggc agtaatctgc tcgatgcgtt ttttcgccgc
    26581 atcgccggct tcttgagtgg tcgcggcaat ggtaatcgta ccgtcttcgg caatattgat
    26641 ttccgtaccg gtttcagcgg taatcgaacg gatggtttcg ccacctttac cgataacttc
    26701 gcggattttg tcttggttga ttttcatcgt aaacaaacgc ggcgcgtgtg cggacaactc
    26761 ttgcgggccc gcaacggcgg ctttcatctg atccaagatg tgcagacgcg cttctttggc
    26821 ctgtgccaaa gcgatttgca taatttcttt ggtaatgcct tggattttga tgtccatttg
    26881 cagcgcggta acaccttcgg tcgtaccggc cactttaaag tccatatcgc ccaagtggtc
    26941 ttcgtcgccc aaaatgtcgg tcaggacggc aaatttgttg ccttccagaa tcagacccat
    27001 cgcgataccg gcaacgtgtg ctttcaaagg cacgccggca gacagcaggc tcaaacagcc
    27061 gccgcagacg gaagccatag aggaagagcc gttagattcg gtaatttcgg agaccacgcg
    27121 catggtgtag ctgaaatctt caggtttcgg caatacggcc aacaatgcac gtttagccaa
    27181 acgaccgtga ccgatttcac ggcgtttcgg cgcgcccatg cggcccactt cgccggtaga
    27241 gtacggcgga aagttgtagt gcagcataaa gcggtcggtg tattcgccgg acagcgcgtc
    27301 gatgatttgc tcgtcgcgcg aagtacccaa agtcgctacg gcaagggctt gggtttcgcc
    27361 acgggtaaac aatgcggaac cgtgcgtgcg cggcaatacg ccggtttgga tgttcagcgg
    27421 acggacggtg cgggtgtcgc ggccgtcgat gcgcggttgg ccgtccaaaa tttggctgcg
    27481 gacgacatcg gcttccaagt gtttgaaaat gcctttgatt tcgttggctg ccagcgtgtc
    27541 ggtttcttcg gtaatcaagg cttcttttac cgcactccaa gcttcgtcca atttggcaga
    27601 acgcgcttgt ttttgacgga ttttgaacgc ttctttaatg gtttcgccgg caatcccgcg
    27661 gactttggca accagttcct cattggtttc aggtgctttc caatcccaaa cttccagatt
    27721 gacttcgtcg gcaaattcat tgattgcatt gatggcaacc tgcatttgat cgtggccgta
    27781 aaccaccgcg cccagcatca cgtcttcggg caggattttg gcttcggatt ccaccatcaa
    27841 cacggctttg gaagtaccgg cgaccaccaa gtccaattgc gatttcgcca attcggcttt
    27901 agtcgggttc aaaacgtaca cgccgtttac ataaccgacg cgtgccgcgc cgatcgggcc
    27961 ggcaaacggt acgccgctca acaccagcgc ggcagacgcg cccaacattg caggaatatc
    28021 agaatcgatt tcaggatcga cggacacgac catcgctacg atttggatgt cgtggtagaa
    28081 accttcaggg aacagcggac gaatcggacg gtcgatcaga cggctggtca ggatttcttt
    28141 ttcgctttgt ttgccttcgc gtttgaagaa accgccggga attttgcctg cggcgtaagt
    28201 gcgctcgagg taatcgacag tcagagggaa gaagtcttgg ccttctttca cttctttgtt
    28261 ggtggtaacg gcaaccaaaa ccacggtgtc gcccatagag actttaacgg cagcggcggc
    28321 ttggcgggca atttcgccgg tttccaaagt aacggtgtgg ttgccgtatt ggaaagtttt
    28381 aacgtgtttg tcgaacatta ttgttccttt caaaataccg cactgctaaa acactaataa
    28441 tgcacactaa aatccgaatg tgcatagtta gggtttcaga ccgtgcggca ggttgtaaac
    28501 aagctttcag acgacatttc ggacgctgaa agcaattcca aattataaag gcaaccatca
    28561 ttaaaatcca gtattgatac aataaaaagg ccgtctgaac catatttctt tcagacggcc
    28621 ttcaaactta aaaatcaatc ctgagtcagc aaagtcaatg cttcccgata cttctcgaca
    28681 gttttctgaa tcacatcggc aggcactttc ggcgcagggg cttttttgtt ccaaccgctt
    28741 tgttccagcc agtcgcggac gaattgtttg tcaaaagacg gcggattggt gccgactttg
    28801 tattggtcgg caggccaaaa acggctcgaa tcgggagtca atacctcatc catcagcgtc
    28861 agcgtaccgt ttgtatccaa accgaattca aatttggtat cgcaaatgat gatgccgcgc
    28921 gatttggcat attccgccgc ttctgtataa agctgaatcg ctttggcgcg cacttccgcc
    28981 gccaattctt ttccgataat gcgttcgcat tcttcaaagc tgatgttttc atcgtgatcg
    29041 ccgactgcgg ctttggttga gggcgtaaaa atcacttcag gcagttgttg cgcttcctgc
    29101 ataccttcag gcagttgaat accgcaaacc gagccggttt tttgataatc tttccaaccg
    29161 ctgcctgcca gataaccacg cacaatcgcc tctactttca ccggagtgag ctttttagcc
    29221 acgacggcgc gtttctctaa agctttggct tcgttttcag gcaaaacatc gtaaaccgtt
    29281 tgaccggtaa agtggttggg cataatatgc accagttttt taaaccaaaa attggaaatc
    29341 tgcgtcagaa tctccccttt gctcggaatc gggtcgtcca aaatcacatc gaatgcagac
    29401 aggcggtcgg aagcgaccat cagcatacgt ttatcgtcga tttcatataa atcgcgcact
    29461 tttccaaaat agatctttac caaaccaatc tcactcattt cgccccccct gaaaatatct
    29521 tgaaaatacc gacccgacac ccgacaggtt tgaatcacaa accaatattc tagccgaagt
    29581 cggcgcaaaa caatacccat ggcacaaaaa accaacccgt caaccgtcgg caaagttttg
    29641 gcactataat accgacagca agtcctacaa tacactttta ccaaaggaaa tacctcatga
    29701 gaatcctatt gacaggctcg aaaagccaac tggcacgctg cctgcgcgac cgtcttccgg
    29761 aagactggga aaccattgcg acggattccg catccctaga cattaccgat gccgatgccg
    29821 tctgcaacat ggtcaaaagt ttccaacccg acgccattgt caacacggct gcctatactg
    29881 ccgtcgacaa agcggaaggc gatgcggcag cggcatttgc cgtcaatgct tccgccgttt
    29941 acaaccttgc cttgtcagca catcgcgccc atgcccgatt catccacatc tcaaccgact
    30001 atatctttga cggtaaaggg aaaagaccct atcaggaaag cgactttacc aatccttcca
    30061 atgtatacgg acaatccaaa accgcaggcg agctgctcgc actgtctgcc aatcccgaca
    30121 gccttatcct gcggacttct tggctgttta gcgaatacgg ggacaacttt gtccgcacga
    30181 tgctgaacct tgcacgggaa cgttcctcgc tgtccgccgt ccacaaccaa atcggctgcc
    30241 cgacctatgc cggcgattta gctgccgcca tcatccgcct tttgcagcaa acgtcgcccg
    30301 tgcgcggcat ttaccactac gccggcggca aatccgtatc ctggtacgaa tttgcccaac
    30361 atattttcca agcggcatcg caacagcaga catccttccc cgttcccgaa ttgactgccg
    30421 tttcagacaa ggaatatccg accgccgccc ccaggcccgc atacagcatt ttggactgcc
    30481 gcaaaatcga aaacgacttc agcatcaaac cgtcagactg gcaaaaagcc cttgcacagg
    30541 tcgtttccaa gctgctctaa tgccgcccgc ctctccgttt ccgccgtcaa gcaccgcctt
    30601 ggcggttttc ttatatagtg gattaacaaa aaccagtacg gcgttgcctc gccttgccgt
    30661 actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataaa
    30721 atctaccgat tacacaaaca catatcatct ttacacaatc atgcttccat caacagtaaa
    30781 acatgatatg attgccaaca ataacatctc acaataaatt ttctaatttt tattgaaaaa
    30841 atcaataaat aagaattctc ccaaccgaca aataaaataa agaaagggtt aatatgcaac
    30901 atcgaagaag actagcaatt taccaagcat ccaaacgtgc ttcctttacc ggcaggtcat
    30961 cagccccgca aaaacgtaaa gaacgttaat ttgaaaaaaa tgccgtctga agtcctgctt
    31021 cagacggcat ttttttaccg ttcgagaaac tgtttcaacc tgtcctcatc caaaaaccag
    31081 tggcagattt cctcatctcc cgccaacaaa acgggaacca gctcattgta tttttcttcc
    31141 aaaacaggat tttcatccac atcgaccact tccagcccga acccgtattc attttgaaaa
    31201 ggtttgagtg catcgcgcat tttgtggcac aagctgcaat attcacgaaa catcaaggtc
    31261 aatttcatcc gcgtttcctt atctgtcaat ttgcacacgc caaagcctta gacgcagcag
    31321 aatcatggtc tatttgggaa aaaacaatgt tttcaaggaa gatgatactc aagtcctgcc
    31381 aaaaacagca aaaatgccgt ctgaacagtt cagacggcat ttccaaaacc gttttacgct
    31441 tgaacattga tacccgccgt aaccgtcggt tgcgcattgt gcgccgtcag aaacgaagcg
    31501 gcaagctccc cacagcagcc gcagcagctc gctacgccca attgcgactg caaatcgccc
    31561 attgtggtcg cgccggcggc gatggtttcc ttgatttgat ggtcggtaac ggcattgcag
    31621 atgcagacaa acattttgtg ctccgtgtgt tctcaaacta tcgtaccgat agcggctttt
    31681 attatcgtat gcgaatataa ataaaaacgg ttcgcattgc aaggtcggta tacacggttt
    31741 gtcttggtaa ttttttatca agtttgcatt ttcgattgat ttcttattaa accaaagtag
    31801 aagcctcagt ttcgggaatg gtgttccttc actacaccct gcaaccatac gttggcaaca
    31861 tgggtataaa tctgcgtcgt attcaaatcg gcatgtccca acatatcctg aaccacgcgc
    31921 aaatccaagc cgtgccgcac cagatgcgtg gcaaaggcgt ggcgcaggct gtgcgggctg
    31981 atgtgcccga tgcctgcctg acttgcatat tctttgacaa tcatccatgc caactgacgg
    32041 gaaatgcccg tctttttctg actgacaaac aatgcgtcgc aattcctgcc tttcagcaga
    32101 agtgggcgtg cctccgtata atagcgttcc acccaatacg ccgactcctg ccccatcggg
    32161 accatcctct gcttatcacc ctttcccagc gcggtaatac agcccctgtc caaatccaca
    32221 ttgccgaagt tcagcccgac cgcctcgctg acgcgcaagc cggtcgcgta catcaattcg
    32281 agcaaagcct tatcccgcaa accgtgcggc gtgtcggtat ccggggcggc aagcagtcgg
    32341 gaaatctgct gctcggtgat cagggtcgga atattcttgt cgattttggg cggtttcagc
    32401 aggcaggtgg gattgtccgt ccttatgcct tcacgctcca tccatatata caggcgtttg
    32461 cacgccgata atgcgcgcgc ctgcgaactc cgttgctctc cgtcaacata aaccgccgcc
    32521 gccaaatccg cttcgtccgc atccttcagc gttctgcccg attgggacag gcggcgggcg
    32581 attttttcca aatcgcgccg gtaaccgttt aaagtattct gactgagccg cctgtccaac
    32641 cacagcgttt caagcagcct gtcgatcaaa ccttcttcca taccgttcca aacaaatgcc
    32701 gtctgaatct tcttcagacg gcatggttca cattatcggg aaagcgtttc caatacttcc
    32761 tgcgcgtgac ccgccacttt gactttccgc cattcatggg cgatttctcc atccttattc
    32821 aagacgaacg tactgcgctc gatacctaac gactctttcc cgtacagttt cttcaatttg
    32881 atgacatcaa acaggcggca cactgtttca tccttgtcac tcaacagctc gaaccggaaa
    32941 ccctgcttgg cgcaaaaatt ctgatgcgcc tttacgccgt cgcgggaaat accgaccacg
    33001 gtataaccca atgcctcaaa ctgttccaaa cgcgcattga aatccaagcc ttccgtcgta
    33061 cagccaggcg tactgtcttt cggataaaaa tacacgacca aaggcagatg ttctgccgaa
    33121 tgaaaatccg cactgctgct cgaaggcagg gtaaattcat atttcacatc catagtccta
    33181 ctcccgatat tcccattatt caaaacggca cgcagacgca ccgccgcaat tgccaaacca
    33241 accccgattc taccgcccca aaggacaagg attcaaccgc cggaaacatc caaaccgaca
    33301 cacgacggca tgaaaaatat ccatgtcaaa ccacaaaata tgttccgatt taaaaacaga
    33361 atgttataaa acccaatccc caaaacacaa caagaccgcc cgcctacggg cagtctcctg
    33421 tcagacgaca tactttacag atggctgttt tttcaacaaa ataacgccaa tactcaaaaa
    33481 tatggaatca aaaatgtcca tccatactct gaaacgcctg ccctcatcgc tgctgctcgg
    33541 tctctgcctt tccctgccgt cagcccacct ttttgccgac aacgacattt tagggcaatt
    33601 tttagaacag aacatgctta cctcctccga tccgatagaa atattcgccg aaagcacaat
    33661 acaccccacc aacacccaag ccattacagg cggtctgatt ctctcctcac agtctgccct
    33721 ggtcgtcaac aacaaaaccg gacagatact gtatcagaaa aacgccgaca ggattatgcc
    33781 catcgcctcc atttccaaac tgatgagcgc gatggtcgtt ttggatgcaa acttggacat
    33841 gaacgaaacc gttaccatta cgcccgacga aatcgaccgc atcaaaggga ccggcagccg
    33901 tcttgccata ggtacggcac ttacacgcaa agaactgctg cacctgagcc tgatgagcag
    33961 cgaaaaccgc gccacccatg cattgggcag aacctacccc ggcggcatgg gcgcatttgt
    34021 cgccgccatg aaccgcaaag cccaaagcct cggtatgtac ggcagccgct tttacgaacc
    34081 gaccggactc aacttccaaa acgtttccac cgccaaagac ctgagcctta tggtcaacgc
    34141 cgccgcccaa tatccgcaaa tccgcaccaa ctcgacttcc aactacgcct cggtacagac
    34201 caaaaacggg cagcagaact acaaaaactc caatgccctg gtcagagaag gcatgtggaa
    34261 catcgaattg cagaaaaccg gctacatacg cgaagcaggc aggtctatgg ttgtcaaagc
    34321 caacattcaa aatcaacccg ttaccatcgt attgctgaac tcgcccacat ccgccacacg
    34381 cgtcaacgac gcccgcaaaa tcgaatcgtg gatgctgcag caacgctcct gacatacaaa
    34441 tgcccggcgg aaaaccgccc aaacacaaaa aaaattggta atttttccta ttcaaggcta
    34501 caattcgaca cgcacagggc gtctgcccga tttcacaatc ataataacgg agttaaaaat
    34561 atgacagcta aaggacaaat gttgcaagat cccttcctga acgccctgcg taaagagcac
    34621 gttccggttt cgatttacct ggtcaacggc atcaaactgc aaggtcaggt tgaatctttc
    34681 gaccaatacg tcgttcttct gagaaacact tccgtcaccc aaatggttta caaacacgcc
    34741 atttccacca tcgtaccggc tcgctccgtc aacctccaac acgaaaacag accccaagcc
    34801 gcaccggctt cgacccttgt ccaagtggaa accgtccagc agcctgccga ataatccgcg
    34861 cgaagcatga cgtgtcatat ctttcaatac cttaccggac aacggtaagg tatttttatt
    34921 ttcagacagc atttaaaaat gttattgcaa aacatccttc cattcgccca ttgccttttg
    34981 cggaaggcac ttcccgaagg tggcaatgct ttggacggca ccgccggcaa cggacacgac
    35041 acccttttcc tcgcacaaac cgcaggcatc cgggggaaag tgtgggcatt cgacatccag
    35101 ccgcaagccc tgagccgcac tcgatgccgt ctgaaagaag cgagatacga gaatgtgcaa
    35161 ctcatcttag acggacacga aaacctgaaa caatatgttt cagaaccact aaatgccgcc
    35221 attttcaact ttggctggct gcccggcgga gataaaagcc ttaccaccca tacggaaacc
    35281 agcattcccg ccctttgcgc aaccctctcc ctgctgaaag aaggcggtat gcttattgcc
    35341 gtcctctatc cgggacacga aaacggcaaa caggaggcag aagcaatcga acaatgggca
    35401 aaaaacctgc ctcaagaaca gtttgccgtt ctgcgttacg gctttaccaa ccggaaaaac
    35461 aacccgccct atctgttggc atttgaaaag ttacgtcaaa aataaatgtt tgcggtaaaa
    35521 taagccgatt ctaaccctta ttttgatacg agccgaccat gcagtatctg attgtcaaat
    35581 acagccacca aatcttcgtt accgttacca ttttgttgtt caacatccgc tttttcctgc
    35641 tttggaaaaa tcccgaaaaa cctttggcgg gcttgtggaa ggcactgccc cacctcaacg
    35701 acacgatgct gctgtttacg ggattgtggc tgatgaaaat cacccatttc tccccgttca
    35761 acgcgccttg gctcggcaca aaaatcctgc ttctgctcgc ctatatcgca ttgggtatga
    35821 tgatgatgcg cgcccgcccc cgctcggtca aattctatgc cgtttacctg cttgccattt
    35881 gttgcgtcgc ctgtatcgtt taccttgcca aaaccaaagt cctgccattc tgaaacactg
    35941 ccatgaacaa cagacatttt gccgtcatcg ccttgggcag caaccttgac aaccccgcac
    36001 aacaaatacg cggcgcattg gacgcgctct cgtcccaccc cgacatccgg cttgaacagg
    36061 cttcctcact gtatatgacc gcgcccgtcg gttacgacaa tcagcccgat ttcgtcaatg
    36121 ccgtctgcac cgtctccacc gatatggacg gcatctcgct gctctctttg ctcaaccgta
    36181 tcgaggcgga tttcgggcgc gaacgcagtt tccgaaacgc gccgcgcaca ttggatttgg
    36241 acatcatcga ctttgacggc ctatccagcg acgacccccg cctcaccctg ccgcatccgc
    36301 gcgcacacga acgcagtttc gtcatacgcc ctttggcaga aatcctccct gattttgttt
    36361 taggaaaaca cggaaaagtt gccgaattgt caaaacaact gggcaatcaa ggtatccgtc
    36421 ttttaccgga taagtaattc cgcacgcgga tgccgtctga atgccggttt cagacggcat
    36481 ttttcctttg ccgccaacac gcgtgcaaaa aaatcgcccc ttggaaaagg ggcgcaaaag
    36541 gaacacaaac cactaccaaa actttaaatc tgcaacattg cctgccgcat actgtatccg
    36601 acaggatata aagccctcac taaatcgttt cgagaaatcc aaacttcttc atcgccgaca
    36661 gaaaatctgc ctttctccgg caccagctcc aacaaaaacg gttgaaccgc cgtatgcagc
    36721 ctgtccttac accgccccag ctttctggac aaggcggaca gggcacgctt atagacacta
    36781 tccttgcggt caagttcccg cgcactgccg acccagctca aacgaaggtt gcggctttca
    36841 tcctcaatca gcaaaatgcc tgttttgact tcccccacct ctggctcgca tgaaagcgaa
    36901 atataatatt tgccgtcgat gttgacatac gccgcctcat gcacctgaca gttgcagccg
    36961 gaagtggcaa gcagcgaata aacagaatcc cgatgcagtt tcaaaatatc aagggaaatc
    37021 tgtttgaaaa tactttccgg catttttccc tcctttcgtc tgctgcgttg tttactggat
    37081 tgactgcatt atacagacat ctcccaatct tgcaaatgat aattgttcct attttttaaa
    37141 aaatttataa attattgatg gatatgtgaa ttaattttaa gatttaaaat ctctttctca
    37201 aaaaaacaca aaaatgccgt ctgaaacgtg ttcagacggc attggctgca cagccagtcc
    37261 gtttaaaatt tcacgccctt atgcagcgcg acgatgcccg cactcatatt gtgataatcc
    37321 acgctgtcga agcccgcatc cagcatcatc tgtttcaaag tttcctgatc ggggtgcata
    37381 cggatggatt cggcaagata ctggtaactc tccgcatctt tcgcaatcag cctgcccatg
    37441 accggcagca gcttgaacga ataaaaatca tacgcgcctt cgagtggctt gtagattttg
    37501 gaaaactcca acaccagcaa cgtgccgcca ggtttcaaaa cacgatacat ctctttcagc
    37561 gcggcatctt tatgcgtcat gttccgcaag ccgaacgcca cggaaactaa gttgaaataa
    37621 ttgtcaggga aaggcagttt ttccgcatcg gcaagcgaca cgggcaaaat catgccttcg
    37681 ttcaacagac ggtcgcgccc gacggtcagc atagaggaat taatatcggt cagccaaacc
    37741 tcgccttcct tgccgacccg tttcgcccaa ccgcgcgaca aatcgcccgt accgcccgca
    37801 atgtccaaca ctttatcgcc ttttttcagg tgcgccgtgt tgatggtgaa atgcttccac
    37861 accctgtgca gccctgccga catgacatca ttcataatgt cgtagttttt ggcgacggag
    37921 tggaacactt ccgccacttt tccggctttc tcatcttcgt tgaccgtact gaatccgaaa
    37981 tgcgttttct gtccgcccat attctaacca cctccctgat gccgccctac ggcatcccaa
    38041 acttaacaat ctgattgctt ttcaaaacgc gacgcgcccg ccgccgccaa tttgtccaaa
    38101 tattcctgcc acatcgcatc gtatccgtat gccagtctgt gcagatacgc ccaatcgtaa
    38161 agaccgctgt cgtgcccgtc cgaaaaactg attttcagcg cgtactgtcc gacaggctgc
    38221 aaatccgtaa tttgcacatc tgccttaccg gtctgcaaaa catcctgtcc cacaccgtgt
    38281 ccgcgcactt ccgcactcgg cgaatagacg cgtaaaaatt cggcaggcag atttttaggt
    38341 tcgtccccat aagtcaaaac caacacggta cggtttttca acaaacggat ttcaacagga
    38401 atattattca aaaaacacct cggttcggca tacaatatcc ggcaggcgca ggcgtgcctt
    38461 caaacggcgc gtattttaac agctttatcc cgataccgat attcaaaccc acactttaaa
    38521 cggcattgat atggacaatc tcgtatggga cggcgttccc gacatccgca cactcgacca
    38581 aaccatccgc aaacacgcac acccgctcaa cctgattgtc tgcctccccg ataatcagat
    38641 tcccgatttt caaaccgcac aagatgcttc ggacgcggaa tgccgtctga agcaccgttt
    38701 ggatcaggca acgcagtgcc tccagttcga cagcatcaac ctcatcgaac acatcctgcc
    38761 cgatgtccgc ttctggctgg ttcccccttc acgcacccgc cgcctgcacg aacacttcca
    38821 ccacatttcc tggcagaccg aagccatccc gcaaaccgaa agcaaacccg acaaaccctg
    38881 gtttgcactt ccacaaacat ccgaacggca aaaaccggaa cacatcctcg ttatcggcgc
    38941 gggcatatcc ggcgcggcaa ccgcccacgc cttagcatca cacggcattt ccgttaccgt
    39001 attggaagcc cgaaaagccg cccaagccgc cagcggcaac cgccaagggc tgctctacgc
    39061 caaaatctcg ccgcacgaca ccgaacaaac cgaactgctg cttgccggct acggctacac
    39121 caaacgcctg ctcggacaca tcctgcccga atccgaaacc tggggcggca acggcatcat
    39181 ccacctcaat tacagccgca ccgaacaaca acgcaatcac gaattgggtt tgcaaaaaca
    39241 ccataaccac ctctaccgca gcatcacgcc ggcagaagcc gaaaaaatcg ccggcatccc
    39301 gctgaacacg ccctacgccg aaccattatg cggactgttt tggcagtacg gcgtatggct
    39361 caatccaccc gcattcgtcc gcaccctcct caaccatccg ctcattggac tacacgaaga
    39421 cacaccgtta accgacattt cccacgacgg ggaaaagtgg attgcaagca cgccaaacgg
    39481 catattttcc gccacacaca tcatctactg caccggcgcg cacagccctc gcctgcccga
    39541 aaccaacctc gccgccctgc ctctcaggca aatacgcgga caaaccggcc tcacaccgtc
    39601 caccccgttt tccgaacaac tgcgttgcgc cgtttcaggc gaaagctaca tcagcccgtc
    39661 gtggcacgga ctgcactgct acggcgcgag ttttattccc aacagcagcc ataccggatg
    39721 gaacgaagcc gaagaagcct caaaccgcca agcattggca caccttaacc ccgccctttc
    39781 cgaatcattg tttgccgcca acccaaaccc ccaaaaacac caagggcacg ccgccatacg
    39841 ctgcgacagc cccgaccacc ttcccctagt cggcgcactc ggcgacattg ccgccatgcg
    39901 gcagacctac accaaactcg cgctggacaa aaactaccgc atcgacaccc catgcccata
    39961 cctgcctaat gcctacgtca acaccgcgca cggcacccgc ggactcgcca ccgcccccat
    40021 ctgcgccgcc gccattgcag cccaaatcct aggcttgccc catccctttt cacaacgcct
    40081 gcgccacgcc ctacacccca accgcaccat catccgcgcc atcgtcagaa ggaaggatct
    40141 aaccccttaa gcctttgccg catgccgtcc cgaccacaga aaatcatatt caaataaaca
    40201 taccaatcct gacggtaaaa tatcgaaaac acccataacg ttttcttccc aaaataagga
    40261 gtaaggaatg cctaacaaat tgcctttgct gtccaagatg aatgttccgt aacattcgaa
    40321 gacttcctta accctgccag ccgtttcggt ttctgtaccc cctgttagat aaggaaggaa
    40381 tgaaaaagcc ctgttgattg tatgaagtaa agggcgttaa tttttgttaa tcttcccttc
    40441 tcaggaactc aatatataag gatgttaatt tttatttccc ttctcgagga c
//
LOCUS       DAXHTY010000004        38894 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXHTY010000004 DAXHTY010000000
VERSION     DAXHTY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38894
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.004"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            307..951
                     /gene="rplC"
                     /locus_tag="ACFNMK_000183"
     CDS             307..951
                     /gene="rplC"
                     /locus_tag="ACFNMK_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC6363530.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKTEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            951..1571
                     /gene="rplD"
                     /locus_tag="ACFNMK_000184"
     CDS             951..1571
                     /gene="rplD"
                     /locus_tag="ACFNMK_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC6363531.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1568..1882
                     /gene="rplW"
                     /locus_tag="ACFNMK_000185"
     CDS             1568..1882
                     /gene="rplW"
                     /locus_tag="ACFNMK_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC6363532.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1888..2721
                     /gene="rplB"
                     /locus_tag="ACFNMK_000186"
     CDS             1888..2721
                     /gene="rplB"
                     /locus_tag="ACFNMK_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC6363533.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2728..3006
                     /gene="rpsS"
                     /locus_tag="ACFNMK_000187"
     CDS             2728..3006
                     /gene="rpsS"
                     /locus_tag="ACFNMK_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC6363534.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3015..3344
                     /gene="rplV"
                     /locus_tag="ACFNMK_000188"
     CDS             3015..3344
                     /gene="rplV"
                     /locus_tag="ACFNMK_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC6363535.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3354..4046
                     /gene="rpsC"
                     /locus_tag="ACFNMK_000189"
     CDS             3354..4046
                     /gene="rpsC"
                     /locus_tag="ACFNMK_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC6363536.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4030..4446
                     /gene="rplP"
                     /locus_tag="ACFNMK_000190"
     CDS             4030..4446
                     /gene="rplP"
                     /locus_tag="ACFNMK_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC6363537.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4446..4637
                     /gene="rpmC"
                     /locus_tag="ACFNMK_000191"
     CDS             4446..4637
                     /gene="rpmC"
                     /locus_tag="ACFNMK_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC6363538.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4637..4900
                     /gene="rpsQ"
                     /locus_tag="ACFNMK_000192"
     CDS             4637..4900
                     /gene="rpsQ"
                     /locus_tag="ACFNMK_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC6363539.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5122..5490
                     /gene="rplN"
                     /locus_tag="ACFNMK_000193"
     CDS             5122..5490
                     /gene="rplN"
                     /locus_tag="ACFNMK_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC6363540.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5502..5825
                     /gene="rplX"
                     /locus_tag="ACFNMK_000194"
     CDS             5502..5825
                     /gene="rplX"
                     /locus_tag="ACFNMK_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC6363541.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5835..6374
                     /gene="rplE"
                     /locus_tag="ACFNMK_000195"
     CDS             5835..6374
                     /gene="rplE"
                     /locus_tag="ACFNMK_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC6363542.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAI
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6377..6682
                     /gene="rpsN"
                     /locus_tag="ACFNMK_000196"
     CDS             6377..6682
                     /gene="rpsN"
                     /locus_tag="ACFNMK_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC6363543.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6698..7090
                     /gene="rpsH"
                     /locus_tag="ACFNMK_000197"
     CDS             6698..7090
                     /gene="rpsH"
                     /locus_tag="ACFNMK_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC6363544.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7104..7637
                     /gene="rplF"
                     /locus_tag="ACFNMK_000198"
     CDS             7104..7637
                     /gene="rplF"
                     /locus_tag="ACFNMK_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC6363545.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7651..8004
                     /gene="rplR"
                     /locus_tag="ACFNMK_000199"
     CDS             7651..8004
                     /gene="rplR"
                     /locus_tag="ACFNMK_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC6363546.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8023..8541
                     /gene="rpsE"
                     /locus_tag="ACFNMK_000200"
     CDS             8023..8541
                     /gene="rpsE"
                     /locus_tag="ACFNMK_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC6363547.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8534..8719
                     /gene="rpmD"
                     /locus_tag="ACFNMK_000201"
     CDS             8534..8719
                     /gene="rpmD"
                     /locus_tag="ACFNMK_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC6363548.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8721..9155
                     /gene="rplO"
                     /locus_tag="ACFNMK_000202"
     CDS             8721..9155
                     /gene="rplO"
                     /locus_tag="ACFNMK_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC6363549.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9167..10477
                     /gene="secY"
                     /locus_tag="ACFNMK_000203"
     CDS             9167..10477
                     /gene="secY"
                     /locus_tag="ACFNMK_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC6363550.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10482..10700
                     /gene="infA"
                     /locus_tag="ACFNMK_000204"
     CDS             10482..10700
                     /gene="infA"
                     /locus_tag="ACFNMK_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC6363551.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10721..10834
                     /gene="rpmJ"
                     /locus_tag="ACFNMK_000205"
     CDS             10721..10834
                     /gene="rpmJ"
                     /locus_tag="ACFNMK_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC6363552.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10900..11262
                     /gene="rpsM"
                     /locus_tag="ACFNMK_000206"
     CDS             10900..11262
                     /gene="rpsM"
                     /locus_tag="ACFNMK_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC6363553.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11282..11677
                     /gene="rpsK"
                     /locus_tag="ACFNMK_000207"
     CDS             11282..11677
                     /gene="rpsK"
                     /locus_tag="ACFNMK_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC6363554.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11697..12317
                     /gene="rpsD"
                     /locus_tag="ACFNMK_000208"
     CDS             11697..12317
                     /gene="rpsD"
                     /locus_tag="ACFNMK_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC6363555.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12343..13329
                     /gene="rpoA"
                     /locus_tag="ACFNMK_000209"
     CDS             12343..13329
                     /gene="rpoA"
                     /locus_tag="ACFNMK_000209"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC6363556.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13353..13721
                     /gene="rplQ"
                     /locus_tag="ACFNMK_000210"
     CDS             13353..13721
                     /gene="rplQ"
                     /locus_tag="ACFNMK_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC6363557.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHEAIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13868..14581
                     /gene="minC"
                     /locus_tag="ACFNMK_000211"
     CDS             13868..14581
                     /gene="minC"
                     /locus_tag="ACFNMK_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC6363558.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14609..15424
                     /gene="minD"
                     /locus_tag="ACFNMK_000212"
     CDS             14609..15424
                     /gene="minD"
                     /locus_tag="ACFNMK_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC6363559.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15428..15691
                     /gene="minE"
                     /locus_tag="ACFNMK_000213"
     CDS             15428..15691
                     /gene="minE"
                     /locus_tag="ACFNMK_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC6363560.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15695..16615
                     /gene="oxyR"
                     /locus_tag="ACFNMK_000214"
     CDS             15695..16615
                     /gene="oxyR"
                     /locus_tag="ACFNMK_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC6363561.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(16612..16845)
                     /locus_tag="ACFNMK_000215"
     CDS             complement(16612..16845)
                     /locus_tag="ACFNMK_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363562.1"
                     /translation="MAIVRQGEATPYWFLLIHYTWHCIGTDENIHSDAKVEYLQDGSF
                     RGDAILKIDDDGNILAYRVVGSGKWRFENNACA"
     gene            17189..17578
                     /locus_tag="ACFNMK_000216"
     CDS             17189..17578
                     /locus_tag="ACFNMK_000216"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6363563.1"
                     /translation="MRYTIKTFQTLADETFPSKNFKILSFNGVARPITIVCPTHGVQT
                     VSAGQAFIRSKHGCPACGLVLSTSVLASRGRSVSILDTATGETLSFPSVQAAAKALNT
                     SYGSIRTKLDGRSSPDNLVCNRYKVMP"
     gene            complement(17949..20786)
                     /locus_tag="ACFNMK_000217"
     CDS             complement(17949..20786)
                     /locus_tag="ACFNMK_000217"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC6363564.1"
                     /translation="MLDKYNPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(20883..21692)
                     /gene="zupT"
                     /locus_tag="ACFNMK_000218"
     CDS             complement(20883..21692)
                     /gene="zupT"
                     /locus_tag="ACFNMK_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFC6363565.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(21873..23129)
                     /locus_tag="ACFNMK_000219"
     CDS             complement(21873..23129)
                     /locus_tag="ACFNMK_000219"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC6363566.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTASRYQ
                     INKERMVRMSEYSREMFRRFEAQTGMNFEGRKKGTLQVFRQTKEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETETGRFEADAVVCALGCFSRTVLAQLDLDLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQALFWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(23208..24599)
                     /locus_tag="ACFNMK_000220"
     CDS             complement(23208..24599)
                     /locus_tag="ACFNMK_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6363567.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKHEDGEDHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWITAIFGMATKYGEGVLAVKYRVTNSKGEMSGGPMYYIENGLGKNWKWMAVA
                     FALFGTLASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIILVMGLLGA
                     GGEFVKPELSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(24956..25732)
                     /gene="lpxA"
                     /locus_tag="ACFNMK_000221"
     CDS             complement(24956..25732)
                     /gene="lpxA"
                     /locus_tag="ACFNMK_000221"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC6363568.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(25819..26268)
                     /gene="fabZ"
                     /locus_tag="ACFNMK_000222"
     CDS             complement(25819..26268)
                     /gene="fabZ"
                     /locus_tag="ACFNMK_000222"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC6363569.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVS
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(26302..27348)
                     /gene="lpxD"
                     /locus_tag="ACFNMK_000223"
     CDS             complement(26302..27348)
                     /gene="lpxD"
                     /locus_tag="ACFNMK_000223"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC6363570.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPSATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(27381..27881)
                     /locus_tag="ACFNMK_000224"
     CDS             complement(27381..27881)
                     /locus_tag="ACFNMK_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC6363571.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(27947..30340)
                     /gene="bamA"
                     /locus_tag="ACFNMK_000225"
     CDS             complement(27947..30340)
                     /gene="bamA"
                     /locus_tag="ACFNMK_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC6363572.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(30397..31737)
                     /gene="rseP"
                     /locus_tag="ACFNMK_000226"
     CDS             complement(30397..31737)
                     /gene="rseP"
                     /locus_tag="ACFNMK_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC6363573.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGGA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(31772..33007)
                     /gene="ispC"
                     /locus_tag="ACFNMK_000227"
     CDS             complement(31772..33007)
                     /gene="ispC"
                     /locus_tag="ACFNMK_000227"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC6363574.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADTEHAARLEALLKHDGTATQVLHGAQ
                     ALVDVASADEVGGVMCAIVGAAGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NSAAVLPVDSEHNAVFQVLPRDYTGRLNEHGIRSIILTASGGPFLTTDLNTFDSITPD
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGVAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(33012..33809)
                     /locus_tag="ACFNMK_000228"
     CDS             complement(33012..33809)
                     /locus_tag="ACFNMK_000228"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC6363575.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMSGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAIMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADVFAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(33812..34558)
                     /locus_tag="ACFNMK_000229"
     CDS             complement(33812..34558)
                     /locus_tag="ACFNMK_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC6363576.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(34614..35171)
                     /gene="frr"
                     /locus_tag="ACFNMK_000230"
     CDS             complement(34614..35171)
                     /gene="frr"
                     /locus_tag="ACFNMK_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC6363577.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAVKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(35351..36478)
                     /locus_tag="ACFNMK_000231"
     CDS             complement(35351..36478)
                     /locus_tag="ACFNMK_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC6363578.1"
                     /translation="MNTSQRNRLSSRWLNSYERYRYRRLIHSVRLGGAVLFATALARL
                     FHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHDN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(36552..36627)
                     /locus_tag="ACFNMK_000232"
     tRNA            complement(36552..36627)
                     /locus_tag="ACFNMK_000232"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36592..36594),aa:Thr,seq:cgt)
     gene            36732..37217
                     /locus_tag="ACFNMK_000233"
     CDS             36732..37217
                     /locus_tag="ACFNMK_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363579.1"
                     /translation="MYVNEKYPYAALFAGLVFLTLPFALSVHDALAFAFGRTGLLVSV
                     SDGGFGWRGGWDSTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSAALGGLRGEAVLEYAFAVWLVAMLTLPKRLTRAPVQLVVFHRK
                     K"
     gene            37313..37936
                     /gene="rsmG"
                     /locus_tag="ACFNMK_000234"
     CDS             37313..37936
                     /gene="rsmG"
                     /locus_tag="ACFNMK_000234"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC6363580.1"
                     /translation="MERKERLQSGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVITSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIDHLPQDVCVEKVQRLDVPGLDAERHIVILRKR"
     gene            38035..38808
                     /locus_tag="ACFNMK_000235"
     CDS             38035..38808
                     /locus_tag="ACFNMK_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC6363581.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSATVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
BASE COUNT         9097 a         8791 c        10732 g        10274 t
ORIGIN      
        1 cagcatacga aaactaccat ctcaaagaaa taatggatgc agtcatcaac catattccgg
       61 tttgtcggag aaagatgcat acgctgtgat gaccggatac cgacccttta aaagagtccg
      121 gccctatgcc gtctgaagat tcaaaacgct tcagacggca tattgaagat atttctgata
      181 tttctgttga tatttctttg acttgtcaga tataatgccg agcttggtac atttgtgcca
      241 agtttaactt tgtctgaaag acaggccaat cgtagcctgt ccctttactt taaaaggaaa
      301 ataatcatga ctttaggtct ggttggacgc aaagttggta tgacccgcgt gttcgacgaa
      361 cagggtgttt ctgttccggt aaccgttttg gatatgtctg ccaaccgcgt tacacaagta
      421 aaatccaaag atactgacgg ctatactgcc gttcaagtta cctttggtca gaaaaaagcc
      481 aatcgtgtca acaaaaccga agccgggcac tttgcaaaag caggtgttga agccggtcgt
      541 ggtttgatcg agtttgcttt gactgaagaa aaactggctg aattgaaagc cggtgacgaa
      601 atcaccgttt ctatgtttga agtcggccag ctggtcgatg taaccggtac ctctaaaggt
      661 aaaggtttct ccggcacgat caaacgtcat aacttcggtg cccaacgcac ttcccacggt
      721 aactcccgtt ctcaccgcgt tccaggctct atcggtatgg cgcaagaccc gggtcgcgtg
      781 ttccccggta agcgcatggc cggccaatac ggcaacacca aagcaactgt tcaaaaattg
      841 gaagttgtcc gtgttgatgc agaacgccaa ctgctgttgg ttaagggtgc tgttccgggt
      901 gcggtcaaca gcgatgttgt agttcgtcct agcgtgaaag taggtgcgta atggaattga
      961 aagtaattga cgctaaagga caagtttcag gcagcctgtc tgtttctgat gctttgttcg
     1021 cccgcgaata caatgaagcg ttggttcatc agctggtaaa tgcctacttg gcaaacgccc
     1081 gctccggtaa ccgcgctcaa aaaacccgtg ccgaagtaaa acactcaacc aaaaaaccat
     1141 ggcgtcaaaa aggtaccggc cgtgcccgtt ccggtatgac ttcttctccg ctgtggcgta
     1201 aaggtggtcg cgcgttcccg aacaaacccg acgaaaactt cactcaaaaa gtaaaccgca
     1261 aaatgtaccg tgccggtatg gcgactattc tgtctcaatt gactcgtgac gagcgtttgt
     1321 ttgcgattga ggcgttgact gccgaaactc ctaaaaccaa agtttttgct gaacaagtga
     1381 aaaatctggg tctggagcaa gtgttgtttg taaccaaaca gctcgacgag aatgtttact
     1441 tggcttcacg caacttgcca aacgtgttgg ttttggaagc tcaacaagtt gatccttaca
     1501 gcttgctgcg ttacaaaaaa gtaatcatca ctaaagatgc agttgcacaa ttagaggagc
     1561 aatgggtatg aatcaacaac gtttgactca agtgattttg gcacctatcg tttctgaaaa
     1621 aagcaacgta ttggctgaaa aacgcaacca aatgacgttt aaagttttgg caaatgcaac
     1681 caaacctgaa atcaaagcgg ctgttgagct gctgtttggt gttcaagttg cttctgtaac
     1741 taccgttaca actaaaggca aaactaagcg ttttggtcgt actttgggcc gccgcagcga
     1801 tgttaaaaaa gcttatgtaa gcttggctgc cggtcaagag ttggatttgg aagccgctgc
     1861 tgcagctgca gataaggaat aaacaaaatg gcaatcgtta aaatgaagcc gacctctgca
     1921 ggccgtcgcg gcatggttcg cgtggtaaca gaaggtttgc acaaaggtgc accttatgcg
     1981 cccttgcttg aaaagaaaaa ttctactgcc ggccgtaaca ataatggtca tatcaccacc
     2041 cgtcacaaag gcggcggtca taaacaccat taccgtgttg tagactttaa acgtaacaaa
     2101 gacggcattc ctgccaaagt agagcgtatc gaatacgatc ctaaccgtac tgccttcatc
     2161 gcactgttgt gctatgcaga cggcgagcgt cgttacatca ttgctcctcg cggtattcaa
     2221 gccggtgctg tattggtttc cggtgctgaa gctgccatca aagtaggcaa caccctgccg
     2281 attcgcaaca ttcccgttgg tacgactatc cactgtatcg aaatgaaacc cggtaaaggt
     2341 gctcaaatcg cacgttctgc cggtgcttct gcggtattgc tggctaaaga aggcgcgtac
     2401 gctcaagtcc gtctgcgctc tggcgaagtc cgtaaaatca acgtaaattg ccgtgcgacc
     2461 attggtgaag ttggtaacga agagcaaagc ctgaaaaaaa tcggtaaagc cggtgccaac
     2521 cgttggcgcg gtattcgtcc gaccgtacgc ggtgttgtca tgaatcctgt cgatcacccg
     2581 catggtggtg gtgaaggccg tacgggcgag gcccgcgaac cggtcagccc atggggtact
     2641 cctgctaaag gctaccgcac tcgtaataac aaacgcacgg ataacatgat tgttcgtcgc
     2701 cgttactcaa ataaaggtta atttagtatg gctcgttcat tgaaaaaagg cccatatgta
     2761 gacctgcatt tgctgaaaaa agtagatgct gctcgcgcaa gcaacgacaa acgcccgatt
     2821 aaaacctggt ctcgtcgttc taccattctg cctgatttta tcggtctgac cattgctgtg
     2881 cacaacggcc gcacccatgt gcctgtgttt atcagcgaca atatggttgg tcataaatta
     2941 ggcgaattct cattgacccg tacctttaaa ggccacttgg ccgataaaaa ggctaaaaag
     3001 aaataaggtg aatcatgaga gtaaatgcac aacataaaaa tgcccgtatc tctgctcaaa
     3061 aggctcgttt ggtagctgat ttgattcgtg gtaaagacgt tgcccaagct ttgaatattt
     3121 tggctttcag tcctaaaaaa ggtgccgagc tgattaaaaa agtattggag tcagctattg
     3181 ctaatgccga gcacaataac ggtgcggaca ttgatgaact gaaagtggta actatctttg
     3241 ttgacaaagg cccaagcttg aaacgttttc aagctcgcgc caaaggtcgc ggtaaccgca
     3301 tcgaaaaaca aacttgtcat atcaatgtga cagtgggtaa ctaaggaaaa gctatgggac
     3361 aaaagattaa ccctacaggc tttcgcctgg cggtaactaa agactgggct tcaaaatggt
     3421 ttgctaaaag caccgacttt tctactgttt tgaagcagga tatcgatgtt cgcaattatt
     3481 tgcgtcaaaa attggccaat gcttcggttg gtcgagtggt tattgaacgc cctgcaaaat
     3541 ctgcacgcat taccattcac tccgctcgtc cgggtgtggt tatcggtaaa aaaggtgagg
     3601 atatcgaggt tttgaaacgt gacttgcaag tcttgatggg tgtacctgtt catgtaaata
     3661 ttgaagagat tcgccgtcct gagttggatg ctcaaattat tgctgacggt attgcccagc
     3721 agttggaaaa gcgcgttcaa ttccgtcgtg ctatgaaacg agcaatgcaa aatgcaatgc
     3781 gttctggtgc taaaggcatt aagattatga cttcaggccg tctgaatggt gcggatattg
     3841 cccgtagcga atggtatcgt gaaggtcgcg tgccactgca tactttacgt gcaaatgtag
     3901 attatgcaac cagcgaagcg cacaccacat atggtgtatt gggtctgaaa gtttgggttt
     3961 atacggaagg caatattaaa tcttccaaac ctgaacatga gagtaaacaa agaaaggcag
     4021 gtagacgtaa tgctgcagcc aactagactg aaataccgta agcaacaaaa gggtcgcaat
     4081 actggcatcg ctactcgcgg taataaggta agtttcggtg agttcggctt gaaagccgta
     4141 ggtcgtggtc gtttgactgc ccgtcaaatc gaagctgctc gtcgtgcaat gacccgtcat
     4201 atcaaacgtg gtggtcgtat ttggattcgt gtattccctg ataaaccgat tactgaaaag
     4261 cctattcaag ttcgtatggg tggcggtaaa ggtaacgtgg aatattacat tgccgaaatt
     4321 aaaccaggta aagtgttgta tgaaatggat ggcgttccag aggaactggc tcgtgaagca
     4381 ttcgagttgg ctgctgccaa attgcctatt cctacaacct ttgtagtaag acaggtgggt
     4441 caataatgaa agcaaatgaa ttgaaagaca aatccgttga gcagttgaat gcagatttgt
     4501 tggacttgtt gaaagctcag tttggcttac gtatgcaaaa cgctaccggt caattaggca
     4561 aaccaagtga attgaaacgt gtacgtcgcg atattgctcg tattaaaacc gttttaactg
     4621 aaaaaggtgc taagtaatga gcgaaactaa aaatgttcgt actttgcaag gcaaagtagt
     4681 aagcgacaaa atggataaaa ccgtaacagt attggttgag cgtaaagtaa aacatccgct
     4741 gtatggtaag attattcggt tatctactaa aatccatgcc catgatgaaa ataatcaata
     4801 tggaattggt gatgtggttg ttatatcgga atcccgtcca ttgtcaaaaa ctaaatcttg
     4861 ggttgtcagt gagctggttg agaaagcacg ttctatttaa gaattaaagc aacgtgcttg
     4921 gaatgggaaa cgaagtattg cagcaaattt aatttgcgtg taaacttcgt ttcctgtctt
     4981 tcagtttctt ctggaagttt cttccctttc ggggtccaag actggtttac ttgaaccgca
     5041 aggtttcatt taataagcag cggctttgct gtaagttatc tgaaagtggt aaattaagtt
     5101 ggttaattta aaggtaataa catgattcaa atgcagacca tcttagatgt ggctgataac
     5161 tctggtgcgc gtcgcgtaat gtgtatcaag gtattgggcg gatctaagcg tcgctacgct
     5221 tctgttggcg atattattaa agtggcagtt aaagatgcgg ctccgcgtgg ccgtgtcaaa
     5281 aaaggcgatg tatataatgc ggtagttgtt cgtactgcta agggtgtacg tcgtcctgat
     5341 ggtgcgttaa ttaaattcga taacaatgcc gccgtgttac tgaataataa acttgaacct
     5401 ttgggtactc gtatctttgg tccggtaacc cgtgaattgc gtactgagcg atttatgaaa
     5461 atcgtttcat tggcacctga agtattataa ggaatggcac gatgaataaa atcattaaag
     5521 gcgatagggt tgtagtaatt gctggtaagg ataaaggtaa gcagggtcaa gtagttcgag
     5581 tgttgggtga taaagttgtt gttgagggcg ttaatgttgt aaaacgccat caaaaaccta
     5641 atccaatgcg tggcattgag ggcggtatta ttactaaaga aatgcctttg gatatttcta
     5701 atatcgcaat cctgaatccg gaaactaata aagcggaccg tgttggtatt aagctgattg
     5761 aaaatgaagg caaagttaaa cgcgttcgtt tcttcaaatc aaatggctct atcattgggg
     5821 cataaggaga taacatggct cggttgagag agttttataa agagacagtt gttcctgaat
     5881 tggttaaaca atttggttac aaatcagtaa tggaagtccc gcgtattgaa aaaatcacct
     5941 tgaatatggg tgtgggtgag gctattgctg ataaaaaagt tatggaacat gctgtttccg
     6001 atttagagaa aattgccggt caaaaaccgg ttgttactgt tgcccgtaaa tctatcgcag
     6061 gttttaaaat ccgtgataac tatccggttg gttgcaaagt aacattgcgt cgtgatcaaa
     6121 tgtttgaatt cttggatcgt ttgattacta ttgcattacc tcgcgtacgt gacttccgtg
     6181 gtgtgagcgg taaatcattt gatggccgtg gcaattacaa tatgggtgtt cgtgagcaaa
     6241 ttatttttcc ggaaattgaa tacgataaaa ttgatgcttt gcgtggtttg aatattacta
     6301 ttactactac agcaaaaacc gatgaggaag cgaaagcttt attgtcattg tttaaatttc
     6361 cgttcaaagg ataatcatgg ctaagaaagc acttattaat cgtgatctga aacgtcaagc
     6421 tttggctaaa aaatatgcgg ctaaacgcgc ggcaattaaa gcggtaatca atgattcgaa
     6481 tgcaactgag gaagagcgtt ttgaggctcg tttgaggttt caatccattc ctcgtaatgc
     6541 ggcacctgtg cgtcaacgtc gtcgttgtgc tttgacaggt cgccctcgtg gtactttccg
     6601 taaatttggt ttgggtcgta ttaaaatccg tgaaatcgcc atgcgtggcg aaattccggg
     6661 tgttgttaaa gccagctggt aataggagta attaagaatg agtatgcatg atcctatttc
     6721 cgatatgttg actcgtatcc gcaatgcgca acgtgctaat aaagcagcgg ttgcaatgcc
     6781 ttcttcaaaa ttaaagtgtg ctattgcaaa ggtattgaaa gaagaaggat atattgagga
     6841 cttcgcagtt tcatctgacg taaagtctat attggaaatt caattaaaat actatgcagg
     6901 tcgtcctgta attgaacaaa tcaagcgtgt atctcgccct ggtttgcgta tttataaagc
     6961 gtctagtgag attccaagtg ttatgaatgg cttgggtatt gctattgtta gtacttctaa
     7021 aggtgtaatg actgatcgta aagcacgttc tcaaggtgtt ggtggtgagt tgttatgcat
     7081 tgtagcctag tggaggaaaa gaaatgtcac gtgtcgcaaa aaacccagtg actgttcccg
     7141 ctggtgtaga agtaaaattt ggaacagagg cattagttat taagggtaag aacggtgaat
     7201 tgtcttttcc tttgcattct gatgtagcca ttgaatttaa tgatggcaaa ttgacttttg
     7261 ttgcgaataa cagcagtaaa caagcaaatg caatgtctgg tactgctcgc gcattagtca
     7321 gcaatatggt taaaggtgtt tcagaaggtt ttgagaaaag attgcaattg ataggtgtgg
     7381 gttatcgtgc tcaagcacaa ggtaaaatct tgaatctgtc tttgggtttt tctcatccga
     7441 tcgtatatga aatgcctgaa ggtgtctccg ttcaaactcc tagccaaaca gagattgttt
     7501 taaccggctc ggataaacaa gttgttggtc aagttgctgc tgagattcgt gcgttccgtg
     7561 ctcctgagcc ttataaaggt aaaggtgttc gctatgtagg cgaagtagtg gtaatgaaag
     7621 aagccaagaa aaaataattg aggttcacta atggataaac atacaacccg actccgtcgt
     7681 gcacgcaaaa cccgtgctcg tattgcggac ttgaaaatgg taagattatg tgtgttccga
     7741 agcaataatc atatttatgc tcaagtaatt agtgctgaag gtgataaagt attggctcaa
     7801 gcctctacat tggaagctga ggtgcgcggt agtctgaaat ctggaagcaa tgttgaagca
     7861 gctgcaatag ttggtaaacg tatcgctgag aaggctaaag cagcaggtgt agaaaaggtt
     7921 gcttttgatc gttcaggttt ccaatatcac ggtcgtgtga aggctttggc tgaagctgct
     7981 cgtgaaaatg gtttaagctt ctaaatattt ggagactttc agatggcaaa acatgaaatt
     8041 gaagaacgcg gtgacggtct gattgaaaag atggtcgcag ttaaccgtgt aaccaaagta
     8101 gttaaaggtg gtcgcattat ggctttctct gcgctaactg ttgttggtga tggagatggt
     8161 cgtattggta tgggtaaagg taagtcaaaa gaagtgcctg ttgcagtcca aaaggcgatg
     8221 gatcaagcac gacgctctat gattaaggta ccattaaaaa atggtacgat tcatcatgag
     8281 gttattggtc gacatggtgc tactaaagta tttatgcagc ctgctaaaga gggtagtggc
     8341 gtaaaagccg gtggacctat gcgtttggtt tttgatgcta tgggcattca taatatctcc
     8401 gccaaagtgc acggatctac taacccatat aatatcgtac gtgcaacatt agatggtttg
     8461 tctaagttgc atactcctgc tgatatcgca gccaaacgtg gcttgacagt ggaagacatt
     8521 ttgggagtta accatggctg aacaaaaaaa gattagggtt acattggtta aaagcctgat
     8581 tggtacaatt gaatctcatc gtgcatgtgc acgcggttta ggtttgcgtc gtcgcgagca
     8641 tacggtagag gttttagata cccctgaaaa ccgtggtatg attaataaaa tcagctactt
     8701 gttgaaagtg gagtcttgat atgtttttga atacaattca acctgctgtt ggtgctacgc
     8761 atgctggtcg tcgtgttgga cgcggtattg gtagtggtct tggcaaaacg ggtggtcgtg
     8821 gtcataaagg tcaaaagagc cggtctggtg ggtttcataa ggtgggtttc gagggtggtc
     8881 aaatgccctt gcaacgacgc ctccctaaaa gaggttttaa atctttaaca gcatcagcta
     8941 atgcacagct tcgtttaagt gaactggaat caattgctgt taatgagatt gatattttgg
     9001 tcttaaagca agcgggtctg attgcatcta cagtctctaa tgttaaagtt attgcttctg
     9061 gtgaaatttc taaggcagtt gctttgaagg gtattaaagt taccaaaggt gcgagagctg
     9121 ctatcgaggc tgttggtggt aagattgaaa tgtaaggttt aatattgtgg ctaatcaaca
     9181 aacgtcatca ggttcatcca aatttggaga tcttaagaaa cgtcttttgt ttctatttgg
     9241 agcattgatt gtttttcgaa ttggtgccca tatacccgta cctggagttg atgctgttgc
     9301 tttagctaaa ttatacgaaa gcgctggaaa cggcatcctg ggaatattga atatgttttc
     9361 cggtgggtcg ttagagcgct ttagtatatt tgcaatagga attatgccat atatttcagc
     9421 ttctattatt gtacagctcg cttctgaaat tttgccatca ttgaaggctt taaaaaaaga
     9481 aggggaggct ggtagaaagg taattacgaa atatacgagg tatggtactg ttttgttagc
     9541 aattcttcaa agtctaggtg ttgcatcttt cgtatttcag caaggaattg ttgtaacaag
     9601 ttcatttgag tttcatgttt ccacggtagt ttctttggta acgggaacca tgtttcttat
     9661 gtggcttggg gagcaaatta ctgaaagggg tatcgggaac ggtatttctt taatcattac
     9721 ggcaggtatt gcttcaggta ttccttcggg tattgcaaag ctggttacac tgacgaacca
     9781 aggttctatg agcatgctta cggcgttgtt tattgtattt ggtgccttat tattaattta
     9841 tttggttgta tactttgaaa gtgcacagcg gaagattcct attcattatg caaaacgcca
     9901 gtttaatggt agggcgggta gtcaaaatac gcatatgcct ttcaagttga atatggctgg
     9961 tgttattccc ccaatttttg cttccagtat tattctattt ccatctactc ttttaggttg
    10021 gtttggttcg gctgatacaa atagtgtttt gcacaaaata gctggattgt tacaacacgg
    10081 tcaattgctg tatatggctt tatttgcagc gacagttatt ttcttttgtt atttttatac
    10141 ggctttggtt tttagcccta aagaaatggc agagaattta aaaaagagtg gtgcttttgt
    10201 tcctgggatt agacctggtg agcagacctc taggtattta gaaaaagttg tattacgttt
    10261 gacattgttt ggagctcttt atattacaac tatttgttta attccagagt tcttaactac
    10321 ggttttaaat gtaccttttt atttgggtgg cacgtctttg ttgattctag ttgttgtaac
    10381 gatggatttt agtacacaaa taaattcgta taggcttact caacagtatg ataagttaat
    10441 gactcgttca gaaatgaaat cattttctcg gaaatagaat tatggcgaaa gaagatacta
    10501 tccaaatgca aggtgaaatt cttgaaactt tacctaatgc aacatttaaa gtaaaacttg
    10561 agaatgacca tattgtattg ggtcatattt ctgggaagat gcggatgcat tacattcgta
    10621 tttctccggg agataaggtc acagtagagc tgacacctta tgatttaact agggctcgaa
    10681 tcgttttcag agcaagataa accaataaaa ggaaaataaa atgcgtgtac aaccatctgt
    10741 taagaaaatt tgccgaaatt gcaagattat tcgtcgaaat cgtgtagttc gtgtaatttg
    10801 tactgatctc cgtcacaaac agcgtcaagg ttaatggaat atttctttta atgtgattct
    10861 gtgatatagt gacacacttt gccctaaaaa ggaaaaaata tggctcgtat tgcaggggta
    10921 aatatcccta ataacgcaca catcgtaatt ggtcttcagg ctatttacgg tattggtgct
    10981 actcgtgcta aattgatttg tgaggctgca aatattgcgc ctgatactaa agcaaaagat
    11041 ttggacgaga ctcaattaga tgctttgcgt gaccaagttg ccaagtatga agtagaaggt
    11101 gatttgcgtc gtgaggtaac tatgagtatc aagcgattga tggacatggg ctgctatcgt
    11161 ggcttccgtc atcgtcgcgg cttaccatgc cgcggtcaac gcactcgtac aaatgcgcgt
    11221 acccgcaaag gtccgcgtaa agcgattgct ggtaagaaat aaattttaag gaattttatt
    11281 aatggctaaa gcaaacacag cttcacgtgt acgtaaaaaa gtacgtaaaa ccgtgagtga
    11341 gggtattgtg cacgttcatg catctttcaa caataccatc attacaatca ctgaccgtca
    11401 aggcaatgcg ttgtcttggg ctacctctgg cggcgctggt tttaaaggtt ctcgtaaaag
    11461 tacaccattt gcagcacaag ttgcagcaga agcagctggt aaagttgccc aagagtatgg
    11521 cgttaaaaat ttagaggttc gtattaaagg tccaggtcca ggtcgcgaat cctctgtacg
    11581 tgctttgaat gctcttggtt tcaagattac cagcattact gacgttaccc cgttgcctca
    11641 taacggttgc cgtccgccta aaaaacgtcg tatttaatat tggagtgatt tgaaacatgg
    11701 cacgttatat tggccctaaa tgtaagttgg cacgtcgcga aggtacggat ttgtttttga
    11761 agagtgcgcg ccgctctttg gattctaaat gtaaaattga ttccgctcct ggtcagcatg
    11821 gtgcaaaaaa accgcgtttg tcagactatg gtttgcagtt gcgtgaaaaa caaaaaatcc
    11881 gccgtattta tggcgtatta gaacgtcagt tccgtcgtta tttcgcagaa gctgatcgtc
    11941 gtaaaggttc taccggcgag ttgctgttgc agttgctgga atctcgtttg gataatgtcg
    12001 tttatcgtat gggtttcggt tctacccgag ctgaagcaag acagcttgtt tctcataagg
    12061 cgatagttgt gaatggacaa gttgtcaata ttccttcttt ccaagtgaaa gctggtgatg
    12121 ttgtctcagt tcgtgaaaaa gccaaaaaac aggtacgtat tcaagaagca ttgggtttgg
    12181 caactcaaat cggcttgccg ggttgggttt ctgtagatgc ggataaactt gagggtgtgt
    12241 tcaaaaacat gccggatcgc tcggaattga ccggtgatat taatgaacag ctggtggtag
    12301 agttctactc taaataatgc tagctcagtg agggacagtt aaatgcagaa tagcacaacc
    12361 gaatttttga aacctcgtca aattgatgta aatacttttt ctgcaactcg tgcaaaagta
    12421 tctatgcagc catttgaacg tggtttcggt cataccttag gtaatgcttt gcgccgtatc
    12481 ttactgtcat ccatgaatgg ttttgctcct actgaagtag ctattgccgg tgtattacac
    12541 gaatattcta ctgttgatgg tattcaggaa gatgttgttg acattttgct gaatattaaa
    12601 ggtattgtgt ttaaactcca tggtcgtagc caagttcaac ttgtgttgaa gaaatcaggt
    12661 tcaggtgtcg tatctgccgg tgatattgag ttgccgcatg atgtagaaat tctgaatcct
    12721 ggtcatgtca tttgtcattt ggctgataac ggtcaaattg agatggaaat taaagtagag
    12781 caaggtcgtg gttatcaatc tgtttcaggt cgtcaggtag ttcgtgatga gaaccgtcag
    12841 attggtgcaa tccagttgga tgcgagcttt tcgcccatca gccgtgttag ctttgaggtt
    12901 gaacctgcac gtgtggagca gcggacggat cttgataagt tggttttgga tatcgaaacc
    12961 gacggttcta ttgatcctga ggaagctgta cgcagtgcgg cacgtatttt gattgatcag
    13021 atgtctattt ttgctgattt gcagggtacg cctgtggagg aggttgaaga aaaagcacct
    13081 cctatcgacc ctgttctttt gcgtccggtg gatgatctgg aattgacagt acgttcagct
    13141 aattgtttga aagctgagga tatttattat attggcgatt tgattcaacg cactgaaacc
    13201 gagcttctta aaacgccgaa tttgggacgt aaatctttga atgagattaa ggaagtattg
    13261 gcatctaaag gtttgacact gggttctaag ttggaagcat ggccacctgt aggcttggaa
    13321 aagccttaat gaagaattaa aggataattg atatgcgtca tcgtaatggc aatcgcaaat
    13381 taaaccgtac cagcagtcat cgtgctgcaa tgctgcgtaa tatggcgaat tcattattga
    13441 ctcacgaagc tattgtaaca actctgccta aggccaagga attgcgccgt gtagtagagc
    13501 cgttgattac attgggtaaa aagccgtcat tggcaaaccg ccgtttggca tttgaccgta
    13561 ctcgcgaccg tgatgttgta gtaaaactgt ttggcgattt gggtcctcgt tttactgctc
    13621 gtaacggtgg ttatgttcgg gtgttgaaat acggattccg taaaggtgat aatgcacctc
    13681 tggcactggt tgaattggtt gacaaaccgg ctgctgagta attttagtca tataacgcca
    13741 tctgccgaaa agcaggtggc gttatttttg caatatctga taggtaatag ggtattggct
    13801 atcatgttta aaatattaat tgaatagcta aggtttgcgc ggtaaactta catcattaaa
    13861 aaattctatg atggtttata taatgaatgc tttcgatata aagtcgacaa agatggacgt
    13921 attgtctata tctttgcata cgtcagactt gtttgatttg gaagatgtgc tggtcaaatt
    13981 gggcaagaag tttcaagagt ctggtgttgt tccatttgtt ctggatgttc aagagtttga
    14041 ttatcccgag tctttggatc ttgctgcatt ggtttcgttg ttttcaaggc atggtatgca
    14101 aattttgggt ctgaagcatt ctaatgaacg ttgggctgct gcggctatga agtatcattt
    14161 gctgttttgt ctgtctcatt cggaaaatgt taaagaactg ggtcaggttg aggtgcagaa
    14221 cacggaggat ggtcagaaag caaggaaaac agtattgatt acatcccctg tccgtaccgg
    14281 tcagcaggtt tatgccgaag atggcgattt gattgttacg ggggcggtca gccagggggc
    14341 ggaattgatt gcggatggca atatacatat ttatgcgccg atgagggggc gtgctttggc
    14401 cggtgccaag ggtgatactt ctgcccgcat atttatccac tccatgcagg cagaactggt
    14461 ttctgtggcg ggtatttacc gtaattttga acaggatttg ccgaaccatc tgcacaagca
    14521 gccggtacag atattgttgc aggataaccg attggttatc agtgcaattg gctcagagta
    14581 attgtttgat atttaaaaag gaaatattgt ggcaaaaatt attgtagtaa cttcaggtaa
    14641 gggcggtgtc ggtaaaacga ctaccagtgc cagtattgcg acaggtttgg cattacgcgg
    14701 atataaaact gcggtaattg attttgatgt gggtttgcgt aacctcgacc tcattatggg
    14761 ttgcgagcgt cgtgtcgttt atgacctgat caatgtcatt cagggggagg cgacgctcaa
    14821 ccaggctttg attaaagata aaaattgtga aaacctgttt attttgccgg cttcccagac
    14881 tcgggataaa gacgctttga cacgcgaggg cgtagaaaaa gtgatgcagg agctgtccgg
    14941 caagaaaatg ggctttgagt atattatttg cgactctcct gccggtattg agcagggtgc
    15001 attgatggcg ttgtattttg ctgatgaagc cattgtaacg accaatcctg aggtttccag
    15061 tgtgcgtgac tccgacagga ttttgggaat tttgcaaagc aaatcccata aggcagagca
    15121 aggcggttcg gttaaagaac atctgttgat tacgcgttat tctcccgaac gtgtggcaaa
    15181 aggcgaaatg ctgtctgtac aggatatttg cgatattctg catattcctt tgctgggtgt
    15241 gattcctgaa tcccaaaacg tcttgcaggc atccaattcc ggagaaccgg tcatccatca
    15301 ggacagcgtg gcggcttccg aggcatataa ggacgttatt gcccgtcttt tgggcgagaa
    15361 ccgtgaaatg cgtttcttgg aagctgagaa aaaaagcttc ttcaaacgtc tgtttggagg
    15421 ataaggtatg tcattaatcg aatttttatt cggcagaaag cagaaaacgg caaccgttgc
    15481 ccgcgaccgc cttcaaatca tcattgccca agagcgcgcc caagaaggtc aggctccgga
    15541 ttacctgccg actttacgta aagagttgat ggaagtcctg tccaaatatg tgaacgtttc
    15601 attagacaat atccgtattt cccaggaaaa acaggatggt atggatgtgc ttgagttgaa
    15661 cattactttg ccggaacaga aaaaggtata ggacatgacc ttaaccgaat tgcggtacat
    15721 cgtcgcagtc gcccaagaac gtcatttcgg cagggcggcg cggcgttgtt ttgtcagcca
    15781 gcccactttg tctattgcca ttaagaaatt ggaagaagag cttgccgtct ctttgtttga
    15841 ccggagcagc aacgatatta ttacgaccga ggcgggggaa cgtatcgttg cacaggcgcg
    15901 taaggtgttg gaagaggcag agcttatcag gcatttggca aatgaagaac aaaacgagct
    15961 ggagggtgcg ttcaaactcg ggctgatttt tacggttgcg ccgtacctgc tgccgaaact
    16021 gattgtctcg ttgcgccgta ctgcaccgaa aatgcctttg atgttggaag agaattacac
    16081 gcatactttg accgagtcgc tcaaacgcgg ggacgttgac gcgattatcg ttgccgaacc
    16141 gtttcaagag ccgggcattg ttaccgaacc cttgtatgac gaaccgtttt tcgtgattgt
    16201 cccgaaaggg cattcatttg aggaactgga tgccgtttcg ccacggatgc tgggtgagga
    16261 gcaggttttg ctgctgacgg aaggcaactg tatgcgggat caggtactct caagctgttc
    16321 cgaattggcg gcgaaacaac gtatacaggg gttgaccaat acattgcagg gcagctcgat
    16381 taatacaatc cgccatatgg ttgccagcgg tttggcaatc agcgtgttac cggcaaccgc
    16441 gctgaccgaa aacgatcata tgctgttcag cattattccg tttgaaggta cgccgccaag
    16501 ccggcgggtc gtattggcgt accgccgcaa ttttgtccgt ccgaaggcgt tgtcggcgat
    16561 gaaggcggcg attatgcagt cgcagcttca cggggtaagt tttatccgcg actaggcgca
    16621 ggcattgttt tcaaaacgcc atttccctga gccgacaaca cggtatgcca agatattgcc
    16681 gtcatcatcg attttgagta tagcatcgcc acggaaactg ccgtcctgaa gatattcgac
    16741 ttttgcatca ctgtgaatgt tttcatcagt gccgatgcaa tgccatgtat agtggattaa
    16801 caaaaaccag tacggcgttg cctcgccttg ccgtactatt gccaaggcgt accagcctga
    16861 tttgttgatg tgtgtactac tgcatgggtt gttaagtcct gcaagtgatt taagtgtgaa
    16921 gattgagtaa tgcccactag tcgcaggcgc ggggggcagg tgctaagtca ttggttatct
    16981 ccttactatc tatctaacta cagggtgtat tatgcgcctc cttgttgttt tgtgcaagtg
    17041 gttttctgat aatttatgta aagatttggt aaagacgtat agcctttttc tttgcattgt
    17101 gcttgcgcag ggtatcaggt aggtctcaaa aaatttgaaa atttttatgt tttcgtataa
    17161 aatcttttgc acttatacgg agtgagttat gcggtacacc attaaaacat ttcaaaccct
    17221 tgctgatgaa actttcccct ctaagaactt caaaattctt tcgttcaatg gggtggcacg
    17281 acctataacc attgtttgcc ctacacacgg ggtgcagact gtttctgcgg ggcaagcatt
    17341 tatccgttcc aaacacggct gtcctgcctg tggtttagtt ttatcaacct ccgttttggc
    17401 ctctaggggt cggtcagtct ccatcctcga caccgccaca ggcgaaacac tctcgttccc
    17461 cagcgtacaa gctgccgcca aagccctaaa cacctcctac ggctctatac gaaccaagct
    17521 cgacggacgt tcgtcccccg acaaccttgt ctgtaacagg tacaaagtaa tgccgtaatc
    17581 tcgaaacttc gtgctataat aaactcctag cttttgcagg agtttgtttt ttatggattt
    17641 aaatgtcgaa atcaagaatt ttggcaaggt tgcccatgct agggtttcca ttcgcccgct
    17701 taccgtcatc acgggttgca actccacact cgcgttcccc agcgttactg ccgcaggcac
    17761 tgctttgggt gtgcattttc agcaaattaa ccacaggctc aagggtcgaa cgtcctgtca
    17821 ggaggtgttg atttctacac cgattatact gtctgcggct tcgtcgcctt gtcctgattt
    17881 ttgttaatcc actataaaag aggccgtctg aaaaacattt ttcagacggc cttgttgatt
    17941 caatcaaatc agtctttcaa cttcgacaac tgagtttgca cttttgccat tttgtcttcc
    18001 aattccgcca aatcggcttt gtctttttcc accaaatgcg ccggggcttt ttcggtgtag
    18061 ccgggtttgg agagtttggc gttgagtttg tccaaggctt tttgcagctt ctcggcttct
    18121 ttgctcaaac gggcggtttc ggcggctttg tcgatttcga ctttcagcat caggcgcgcg
    18181 ccgttgcaga cggcgacggg cgcgtcttcg ctttcgggta gggcggcgac ttgccgtgct
    18241 tcggtcaggc gggtcatcat cggcaggtat ttgaggtagt ccgccaagtc gtccgcgctt
    18301 tcgacaaaca gcggagcttt gacattaggc tggatgccca tttcgccgcg caggttgcgg
    18361 actgcgccaa tcaaatcctg caacacggtc atttgctcga atgccgtctg aacaatatcg
    18421 ccgccgtcgg cttcggggaa gcgggcgagc atgatgctgt cggcggtttt cgcgtcgcac
    18481 ataggagcga cggtttgcca cagttcttcg gtaatgaacg gaataatcgg gtgcagcagg
    18541 cgtagggcgg cttcgagtac gcgcaataag gtatggcgtg tggcgcgctg gcggctggcg
    18601 cagccggttt gaagctgcac tttggcgagt tccaaatacc agtcgcaata gtcgttccat
    18661 acgaagctgt acagggtttc cgccgccaaa tcaaagcggt aggtttcgta ggcttgcgta
    18721 acctgttcga tggtctgatt cagacggcct atgatccaca tgtctgggaa agagtagccg
    18781 cgaggctcgg tggcggttgc tccgtagccg cagtcttggt tttcggtgtt catcaagacg
    18841 aagttggtgg cgttccagat tttgttgcag aagttgcggt agccttcggc gcgtttgaag
    18901 tcgaagttca cgctgcggcc gaggctggcg tagctcgcca tggtaaagcg caatgcgtcc
    18961 gcgcccatgc tggggatgcc ttcggggaag agttttttgg tggcttcttc cactttcggt
    19021 gcggtttcgg gtttgcgcag accggttgta cgttttacca gcagtttttc caagtcgatg
    19081 ccgtcaatca aatccacagg gtcgatgacg ttgccttcgg atttggacat ttttttgcct
    19141 tcgtggtcgc gcacgatgcc gtggatgtac acgtctttaa acggtacttt gccggtaaag
    19201 tgggtggtca tcataatcat gcgcgccacc cagaagaaga tgatttcgta gccggttact
    19261 aagacattgg acggcaggaa ggctttgagt tcgtcggttt cagacggcca gccgagtgtg
    19321 gagaacggca caagcgcgga ggagaaccat gtatccaata cgtcttcttc gcgagtcaag
    19381 cctgttttgc cggcttgttt ttcggcttct tcctgattgc gggcaacata cacattgcct
    19441 tcgttgtcgt accatgcagg gatttgatgg ccccaccaca gttggcgtga gatacaccag
    19501 tcttggatgt tgttcatcca ttggttgtag gtgttgaccc agttttcggg gataaagcgc
    19561 accgcgccgc tgtcgacggc ttttttggct ttgtcagcaa ggcttaagcc tttgaattcg
    19621 ctgtcaggct cgccgccgtt tggcgtggcg gacatggcga caaaccattg gctggtcagc
    19681 atcggctcaa tcacggaacc ggtacggtcg cctttcggcg tcatcagcgt gtgcgctttg
    19741 atttcgacca agaaaccttg ttcctgcaaa tcggcaacca tttgtttgcg cgcggcaaag
    19801 cggtccaagc ctgcgtattt ttcaggcagg gcaaagcttg gttgcgcttc gcctttgaag
    19861 ttgaacactt cggcgtttgc caatactttg gcttccaagt cgaacacatt aaccaagcgc
    19921 gtatcgtggc gtttgccgac ttcgtagtcg ttgaagtcgt gcgcaggcgt gattttcacg
    19981 cagccggtgc cgaagtcttt ttcaacgtat tcgtcggcaa tcacgggaat ggtgcggccg
    20041 gtcagcggca ggattaattc tttgccgatt aagtgggtat aacgttcgtc ttcgggattg
    20101 acggcaacgg caacgtcgcc caacagcgtt tcgggacggg tggtggcgac gatgacggct
    20161 tcggcaggat tgtccgccag cggatagcgg atgtgccaca tagagccttg ttcttccacg
    20221 ctttccactt ccaaatcaga cactgcggta cccaataccg gatcccagtt caccaagcgt
    20281 ttgccgcgat aaatcaagcc ctgttcatac aggcgtacga acacttcggt tacggtttcg
    20341 gcgcgtacat cgtccattgt gaaatactcg cgcgtccagt cggcagaaca gcccacacgg
    20401 cgcatctgct gcgtaatcgt gccgccggaa acttctttcc attcccacac tttttccaag
    20461 aatttttcac gacccaagtc atgacgggac acgttttgcg cggcaagctg acgctcgacc
    20521 acgatttgcg tggcgatgcc cgcgtggtcg gtgccgggaa tccaggcggt gttgcagcct
    20581 ttcatacggt agtagcgggt caggccgtcc ataatggttt ggttgaaggc atgacccatg
    20641 tgcagcgtgc cggttacatt gggcggcggc agttggatgg aaaaagacgg tttggtcaaa
    20701 tccatatcag gttggaaata gccttggctt tcccagtttt gatagtgctt ggattcgatt
    20761 tcggctggat tgtatttgtc taacatgatg gaactttgtg aaattaaggt tatttttgat
    20821 gtgcggatta taacgcaaaa aggccgtctg aatcatttca gacggccttt ggcatacagg
    20881 ttttaaaaat ggaacaatac caggctgacg gcaatcaccg ccatgcccat tgtcaggccg
    20941 taaacggttt catggccgtc tgaatagcgt ttggcagccg gcagcagctc gtccaacgcc
    21001 aaaaacacca tcacaccggc tatcacgccg aataccgaac caaacacggc aggcgacaaa
    21061 aacggctgca aaaccaaata gcccaaagcc gcccccaacg gctcggccaa gccggatagc
    21121 agacacgccc acaccgtttt cttacggctg cgggtggcaa aataaaccgg cgcggcgatg
    21181 gaaatgccct ccggaatatt atggatggca atcgccaagg ccaaaggcat cccgactgct
    21241 ggattttcca atgtggcaaa aaacgtcgcc aagccttcgg ggaaattgtg cgcagtaatc
    21301 gcaaacgccg ccatcatgcc gactcgcgcg atatggcggc gtttgctttc ttgaaacgac
    21361 gggtcttgcg cgtctaaagt ttcatgcggg ttcggcacca gacggtcaat cagcgcaatg
    21421 ccgcccatcc ctgccaaaaa tgccatggtc gccgccgcaa acgcgtggtc tttatcataa
    21481 atttcagcga acgcctcgct ggacttactg aaaatctccg tcagggaaac atacaccatc
    21541 gcaccgccgg caaatgccaa accaaacgac aacacgcgcg gattgggcgt tttggaaaac
    21601 atcaccaagc cgctgcctaa tacggtaaac aaaccggcag ccaatgtgat ggaaaaggca
    21661 acggccaaat tggacatcga aaaatcgggc atgagaaaac ctgcgctaaa agctgggaca
    21721 ggttcagact aacacttttt aatgtatatg ataatagtta ttatttattt tattgattgg
    21781 ataaccgaat tttgaaacaa aaggccgtct gaaaaatgat tttcagacgg cctttaaatt
    21841 tgaaatgtcg ctaaacctta tcgctttcca gcttaagcct gatagcgcga caggctcaaa
    21901 tcgtcgcttc ggatttcggt gtctttgccg ctcacgatat cggcggttaa ttttgccgaa
    21961 cccagcgaca tggtccagcc caaagtgccg tggcctgtat tcaagaataa gttttcaaag
    22021 cgggtgcggc cgattaacgg cgtgctgtcg ggcgtcatcg gtctgaggcc gctccagaac
    22081 aatgcttggc tcaaatcgcc gccttccggg aacaaatcgt tgacgaccaa agccaaggtt
    22141 tcgcggcgtt tttcgggcag tttgatttcg tagcccgaca attccgccat gccgccgacg
    22201 cggattctgt tgtcaaagcg cgtgatagcg actttgtagc tttcatccaa aacggtggac
    22261 accggcgcgc cgtctgaatt ggtgaccggc agggtcaagg aatagccttt gacgggataa
    22321 atcggcagat cgaggtctaa ctgcgccaac acagtcctac tgaagcagcc gagcgcacag
    22381 acaacggcat ctgcttcaaa ccgccctgtt tcggtttcaa cggctttgat gcgcagcccg
    22441 ttatggtcga tgcggctgat ggtttggttg aaatgaaact gtacgccctt ttcctgacac
    22501 aatttgtata ggttttcagt gaagaggtgg cagtcgccgg tcgcatctgc aggcaggtgc
    22561 aggccgccgg caattttggc ggtaacgcgt gccagcgcag gctcaaattc tgcacattct
    22621 tcgggtttca gacggcggta cggcacgccg tagcgttcca aaacggcaat gtcttgtttt
    22681 gccgcttcga cttctttggt ttggcggaaa acctgcaacg tccctttttt gcgtccctca
    22741 aaattcatgc cggtttgcgc ttcaaaacgg cggaacattt cacggctgta ttcggacatc
    22801 ctgaccatgc gctctttatt gatttgatag cgggatgccg tgcagttttg cagcatccgc
    22861 cacagccatt cgatttgata caggctgccg tcggggcgaa acagcaaagg cggatggctt
    22921 ttaaacagcc atttcagtgc tttggtcgga ataccgggtg cagcccaagg cgtggtatag
    22981 ccgtaagaaa gctggcctgc attggcaaaa ctggtttcca tcgccacacc ctcggcgcgg
    23041 tcgatgaccg ttacttcatg tccggcctct gccagatacc acgcggaaga cacgccggca
    23101 acacccgcac ctaaaacaag cactttcatg tttctccctc cgactttttc aaaacagact
    23161 taatatgccg tgccgtctga atattcggat tcagacggcc tcggatatta atgcggcaat
    23221 tcgccgtttg tgattttttg tttgaagtcg cgcgtttcat tgacgatgac tttcgccatc
    23281 aataaaagtg caatcaggtt gggcaatgcc atcaagccgt tgaatgtgtc cgaagccagc
    23341 cacaccaaat caaggctcaa cacggtaccc agcataacgg aagaaacata acccacgcgg
    23401 tacaaaccgg caaatttctc gccgaaaaca tacaccgcgc atttttcgcc gtaatagcac
    23461 cagcccaaaa tggttgagta ggcaaagaaa atcaggccga tggtaacaat ccagccgccg
    23521 atgccgggca gcattttttg gaatgtgacg gttgtcagtg ccgcgccgct caattcaggt
    23581 ttgacaaact cgccgcctgc gccgagcaga cccatcacca aaataatgcc ggtaatcgag
    23641 caaacgacga tggtgtccaa aaacgtaccg gtcatggaaa ccaaagcctg acggacgggg
    23701 tggtcggttt tcgcggctgc ggcggcaatc ggtgcagaac ccatacccgc ctcattggag
    23761 aacacgccgc gcgccacgcc gtagcggatg accgtaccga tagcgccgcc cgccactgcc
    23821 tgcgcgctga acgcatcgga gaaaatcagc ttgacggcgg gcatcagtgc atcggaatta
    23881 atcgcgataa tggaaagacc gcccaacaca taaaacaccg ccatagcagg cacgatgaaa
    23941 gaagcggctt tggcgatgcc tttaatacca cctaaaacga caacggcagt cagaacggtc
    24001 aacgtaatgc cggtataggc aggttcgata ccgaagctgg tttgcaccgc ctgtgcaacc
    24061 gagttggact gcaccgagct gccgataccg aaggaagcga gtgtgccgaa cagcgcaaat
    24121 gcgacggcca tccatttcca gtttttgccc aagccgtttt cgatgtaata catcgggccg
    24181 ccggacattt cgcctttgga gttggtgacg cggtatttca ccgccaacac gccctcgccg
    24241 tatttggttg ccataccgaa aatggcggtt atccacatcc aaaataccgc gcccgggccg
    24301 ccggttacca ccgcagtcgc cacgccggcg atgttgcccg taccgatggt ggcggacagc
    24361 gcggtcatca acgccgcaaa atgggaaata tcgccttcgt ggtcttcgcc gtcttcatgc
    24421 ttctttgacg gcacaaacgc ctgtttcagc gcatagccca acatagtgaa ctgcaagcct
    24481 ttcagcataa cggtcagcac aatgccggtg ccgaccagca gcatcaacat aacagatccc
    24541 cagacccagc cgctgacggt ttcgaaaaag gctttgagat tgtctaaaaa caattgcatg
    24601 gctttctcct ttgtctgttt tatttttaaa acaccacttt tgtagtgtcc tgtaatttca
    24661 gcacagaata ttcaataaga caatatgttc ttttgaaaaa tacttttggt tttttctccg
    24721 aaagcaggtc ggttcaagct gcggaaattg tttgcaattc tttaaaagca gcggcggagg
    24781 tcacaatgaa atgtccgaat ggggatgtgg cgggcggcag aaatcatcaa tgctgccgac
    24841 tgccatactt ctgaaatcta caaaatgatg catcgatcaa acaatatacc gctttaaaaa
    24901 aaccgatgcc gtctgaaacg ctttcggggt ttcagacggc atcaaaaggg tacggtcagc
    24961 ggatgatgcc gcgcgccgat tgtgcgaaaa agtctcggaa tacggcaagc tcggcttggg
    25021 tttcggcgcg gcggagaatg tctgccttgg cttcttcaaa cggaatgccg cgatggtaga
    25081 gggttttgta cacgtctttg acggcggaaa tctgctctgc ggtaaaaccg ttgcggcgca
    25141 taccttcgct gttgagcccc gccggttcgg cgcggtagcc cgatgccata aagtagggcg
    25201 gcacgtcttt gtgtacgcct gcggcaaacg cggtcatggc gtagtcgccg atgcggcaga
    25261 attggaaaac cagcgtgtag ccgcccaaaa cgacgtagtc gccgacggta acgtgtccgg
    25321 caagcgaggc gttgttggcg aagatggtgt ggttgccgac cacgcagtcg tgcgcgaggt
    25381 ggcagtacgc cataatccag ttgtcgtcgc cgatacgggt ttcgccgatg ccggttaccg
    25441 tgcctaaatt aaaggtggtg aattcgcgga tggtgttgcc gttgccgata atcagcttgg
    25501 tcggctcgtc gcggtatttt ttgtcctgcg gaatttcgcc gaggctggca aattggaaaa
    25561 tgcggttgtt ttcgccgatg ctggtgtggc cgttgatgac ggcgtgcgga ccgatttcgg
    25621 tattcgcgcc gatttggacg ttggggccga taacagtgta cgcgccgact ttgacgccgg
    25681 agtcgagttc ggctttgggg tcgatgacgg cggtcgggtg gatgagggtc atgtttttcc
    25741 tttcctgtcg tgttgccgcg aagatgcgcg acggcaacag gtcgtctgaa aactttcaga
    25801 cgaccttttt ctgaacactc aaaccacgcg tttggcacac atgatgatgg cttcgacggc
    25861 gacttgcccg tccactttgg caacggcgtt gaatttgccg atgccgcgcc ggctggtcag
    25921 cagctcgact tcaaagacga gttggtcgcc ggggatgact tggcgtttga aacgggcttc
    25981 gtctatgccg gcgaagaaga agaattcgtt ttctttgcgc ccgccttcgc tcaaaatcgc
    26041 caacgtgccg caggcttgcg ccatcgcttc gatgatgagt acgccgggca tcacgggcag
    26101 gtcgggaaag tggccttgga actggggctc gtttatgctg acgtttttaa tcgcggtcag
    26161 ggttttcatc ggctcgaagg cggtgatgcg gtcgagctgg agaaacggat agcggtgggg
    26221 gatgagtttt tggatgtctt tggcttcgat ggggagttgt acgtccatgt ctgtcgtatt
    26281 ccttgaataa agtcggtttg gttatttgct gtcttgaccg gcatctgaaa gctgctgctc
    26341 cagtgttttg agccgtttgt tcatttcgct taagcggtgg atgtaaacag cgttgcgcgc
    26401 ccattcttta tgggtggaca tcgggaagat gccggcgagg tgtttgccgc tttcggtaat
    26461 gctgtgggtg acggacgtgc cgccgccgat ggtggttttg tcggcgattt cgatgtgtcc
    26521 gaccgtaccg acgccgccgc cgatgatgca gtagctgcct atggttacgc tgcctgagat
    26581 gccggttttg gcggcgatga cggtgtgcga accgattttg cagttgtgtc cgatttggac
    26641 ttggttgtcg attttggtgc cgttgccgac ggtggtgtcg ctcatcgcgc cgcggtcgat
    26701 gttggtgttc gagccgattt ctacgtcgtc gcccagcgtt accgcgccgg tttgcgggat
    26761 tttaaaccat gaatcgccgg caaaagcgag tccgaaaccg tccgcgccga tgaccgcgcc
    26821 gctgtggatt tcgacgcgtc tgcccagtgt gcagccgtaa taaacgacgg cgttgggatg
    26881 caggacgact tcgtcgccca gtttgcaatc gtgttggacg acggcgtttg ccaagatgcg
    26941 gcagccttcg ccgagcacgg tgtttgcgcc gatgtaggcg ttcgcgccga tttcgcagct
    27001 tgcgggaacg gtggcactcg gttcgacgac ggcggtcgga tggatgccgc cgcgcgcttt
    27061 gacgacgggt gaaaacaggc gggcgacttt ggcgaaatag agataggggt cgtcggcgac
    27121 aatcaggttg cgcccttcaa atccgtctgc cgctttggcg gaaacgatga ccgcgcccgc
    27181 gctgctgtcg tggacttcgg ctttgtattt cggattggca aggaagctga tgtgttccgc
    27241 ctgcgcgtct gcgagcgggc gcacggcggt aacggaaatg tcctcgccgc gccattcgcc
    27301 gccgagccgc gcggtgattt gggacagggt gtaggtggcc ggaatcatgg ttttcctgtt
    27361 cggtatgccg tctgaaaggg tcagcgggcg ttcatttctt taatgacgct gtcggtaacg
    27421 tcgtattggg tgttgacgta aatcacgttc tgcaaaatga catcgtaacc ttcctgtttg
    27481 gcgattttga cgatgacgcg gttggcgttt tgctggaggg aggcaaactc ttcgttgcgg
    27541 cggaggttgt agtcttcttc aaactgcgcc tgttttttgc ggaacgctgc gaccagcccg
    27601 cgccattttt cttcggcttg cgcctttttt gcgtttctga gtttgccttc ggcaagctgc
    27661 ctttccaaat ccagaccttc gcgttgcagt ttttgcaatt cgtcctgacg ggcggaaaat
    27721 tcgctgtcca gcgttttttg aatcttgcgc gcctgcttgg attcgaggta gatgcgctcg
    27781 gtgttgataa agccgatttt ttggaaggtg tcggcgtgcg cgcctgcggt gcagcacaaa
    27841 ccgatcagag ccgcggcaaa cgcgcgggtc aaacgggtca tggtaaaact ccttcgaatg
    27901 ttgccgcgaa atgccgtctg aagggcttca gacggcattt gcgggattag aacgtcgtgc
    27961 cgagttggaa ttggaagcgt tggatttcgt cttccggttt tttcttcagc gggtaggcgt
    28021 agctgaattt catcgggcct aaaggcgaga gccaggtaac cgcgccgccg gcggaatagc
    28081 gcaattcgtt ggtaaaggtg gatttatggg tattgccggc gccgtaaatg ttttgaaccc
    28141 tgccgccggt cgcggaactg ctgttgtcgt cgtaggtttt gccgtcccac acgctgcctg
    28201 cgtcggcaaa caggctcagg cggacggtgc gcgcgtcttt cgcgccaggc atcgggaaga
    28261 gcagctcggc ggagacgttg gcttttttgt tgccgccgta gctgattttt tcgccgtatt
    28321 cgtcatacac tttcggaccg agcgtgccgc tttcgtatcc gcgcaccgaa cccaggccgc
    28381 cgccgtagaa gttttcaaag aaggggattt ctttggttct gccgtagccg cccgcaatgc
    28441 cgacttcgcc gccgagcatc agcgtgaagg ttttgcttaa ggggaagaac caggtttggt
    28501 tgtgggtggc ggagtagtat tgcagtttgc tgccgggcag ggcgatttcg gcgttcacgc
    28561 ccgtcaggta gccgcgcgtc ggccataacg cgctgtcggt tttgttgcgc ccccagccga
    28621 cggtaccttt gtacagccag cctttgaagc tgccgtctgt gccgtcggtt ttgccgtatt
    28681 tcttgataaa gtcggcatag tgtttgggcg ctttgttgta ggtgttgacg gtcaggtgtt
    28741 ctgccaccaa accgaaattc acgcggtcgt attcggtaac aggcacgctc atgcggatgc
    28801 ctgcgcctgc cgtggtggtt ttatattgtt tgatgctggt cgatgctttg cgcgggtcga
    28861 aggcttttcc gtaaacatcg tagcccaggc tgaccccgtc tgccgtgaag tacgggtcgg
    28921 taaacgacag cgagccgttg agcgtggttt tgcttcgtga ggcgcgcagg gcggccgact
    28981 tgcccgtacc gaacaggttg tcttgggaaa cgcctgcgga catgaccagg ccggtatcct
    29041 gtacccagcc cgcgctcaag tcgagcgagc cggtggaacg ttcggtcagg ctcatgttca
    29101 aatcgacttt gtcgggtgtg ccggcaagcg ggacggcatc aaactgtacg ttgtcgaagt
    29161 agcccaaaag ctcgacgcgc tctttggagc gttgcagctt ggaggtgtcg taaggcgcgg
    29221 attccatttg gcgcaattcg cggcgcacga cttcgtcgcg ggttttgttg ttgccggtga
    29281 tgtggatttc gttgacgtag attttccggc ccggttcgat gtgcaggacg aaatcgacgg
    29341 ttttggtttc ggcgtttggc agcggctgta cgctgatttc gctgtatgcg tagcctgccg
    29401 agcccatgcg gttctgaatc tcacccaaaa cggcggtcat ctgctggcgt tcgtaccatt
    29461 tgccgggctt catggtcagc agtttttcca gttcggcttt ggggacttcg ttggtgtcgc
    29521 cttcgatgga gactttgccc caacggaaac gtccgccttc gtggacggtg attttgatgg
    29581 tctgcttggt tttgtcttcg ttggtttgga tgtcggtatc gaggatacgg aaatcgaagt
    29641 agccgttgtt ctggtagaag tcggttactt tttccatgtc ttgggcaaat ttctgctcgt
    29701 tgaattggtt gcttcgtgtc agccatgtcc aaatgccgcc ttcggtcagc gacatctgcc
    29761 gcatcagttt gcggtcggaa tagacttggt tgccttcaaa ttcgatgtcg gtgattttgg
    29821 cggatttgcc ctcgtcaatc gtgatgtcga tgtcgacgcg gttgcgggcg agtttggtta
    29881 ctttgggcgt gatttggata ttgagtttgc cgcgcccgag gtattcttct ttcaggccgg
    29941 cgactgcctg attgagtgtc gcctgattaa agtattgcga ctgcgccagc ccgaacgatt
    30001 cgaggttttt cttaatggcg tcgttttgca gcatttttgc gccggtgatg ttgagcgagc
    30061 cgatggtggg gcgttcgata acggtcagca ggagctgccc gtccgcagtt tcgacgcgta
    30121 cgtcgtcaaa gaaaccggtg gcgtacaggc ttttgatgat ggcactgccg tgtgtgtcgt
    30181 tgtaggtatc gccgactttg acgggcaggt agttgaatac ggtgctcggc tcggtacgct
    30241 gcaagccttc gacgcggatg tcttggatgg tgaagtcggc aagtgccaaa ggcgatatgc
    30301 ccaagaccat cagtgcggaa gcaatctgtt tcagtttcat tgtcagttcc ttgtggtgcg
    30361 gaatgcggtt tcagacggca ttccgaaacg taaaatctaa ccgagcagcc gggtaacgtc
    30421 gttgaagaag gcgaccgcca tcatcagcat catgagggca agcccgaagc gcaaaccgat
    30481 gttttggacg cgttcgccca aaggtttgcc gcgtatccat tcggcagtat aaaacacgag
    30541 gtggccgccg tccaaaacgg ggacgggcag caggttcagc acgccgaggc tgatgctgac
    30601 cagtgccaaa aattccaaat aactttgcaa gccgagttcg gcggactgtc cggcaatgtc
    30661 ggcaatggtc agcgggccgg aaatatggct gacggaggcg ttgccgctga ttagtttgcc
    30721 gaaaaatttg agggttgtcc acgagtggga aacggttttt tcccagccca tgccgaatgc
    30781 gcggataaca gacggacggt agctgcggcg gatttgcgcg tcccacgccc tgtccggctg
    30841 cggacggagg ccgacgcgcc cgatcagggt gtggtcggac tgttcgacag tatcggggcg
    30901 gatgtcggcg gtatgggttt gtccggcgcg ttcgtaggtc agggtgattt ttttgccggg
    30961 gctttggcgg gtcaggtttg cccattcctg ccatgaggcg atgggtttgc cgtcggcggc
    31021 agtcagcctg tcgcccggtt tcaggcctgc tttttcggcg gggctgcctt tttccacgcc
    31081 gccggcaacg gttgtgattt taaagggcat cagtccgatg tagccttggt tttttgcgat
    31141 tttaccggct tccggcgtgc ctgcggcatc gatggtgcgg acggtttgcg cgcccgatgc
    31201 cgtctgaacg ccgacggcga ctttgccggc ttcgaggttg aggacgattt cggtttgcgc
    31261 gccgccccaa tctgcaacgg gtgtgccgtt gacggattgt attttgtcgc cgctttggaa
    31321 gccggcgcgg gcggcaatgg tgtcgggttc gactgtgccg acgtaggggc gcagttcggt
    31381 tacgccgaag gaaaagctca gtccgtacag caaaaccgcc agtgcgagat tggtcagcgg
    31441 gccggcggcg acgatggcga tgcgtttggc ggggtgttgt ttgtcaaaag cgtagggtaa
    31501 atcggcttct gatacttcgc cttcgcgcgt atcgaccatt ttgacgtaac cgcccaacgg
    31561 aatcggggcg aggcaccatt cggtgtcgcc gcgctttcgg gtgaaaaacg gtttgccgaa
    31621 gccgacggaa aagcgtacga ctttgacgcc gcacaatctg gcaacgatgt agtgtccgaa
    31681 ctcgtgcagg ctgaccaaaa tcaggatggc gaagataaaa gctagaaggg tgtgcaaatg
    31741 gttttccttt gataacggtg ttcagacggc atcagcgcag tgtgccgata aatgcccgcg
    31801 cttgtgcgcg tgtccgggca tcttgcgcca agagcccccc tatgtcgcct atgccgtctg
    31861 aaaagtcttg tgaaagacaa tgggcgacgg ttttggcaat gtcggtaaac ttaatctgtc
    31921 cgtccaaaaa ggcggcgacg gcggcttcgt tggcggcgtt caatacgcag ggcgcgaccc
    31981 cgcccgcgtt catggcttca taggcgagct tcaggcaggg gaagcggtca aagtcgggct
    32041 tttggaaggt cagcgcggac aatgcgtcga aatccaggtc gccgacaccc gaatcgatgc
    32101 gctcgggcaa acccaaacaa taagcgatgg gcgttcgcat atcgggattg cccagttgcg
    32161 ccaacacgga gccgtcgcgg tagcgcacca tgctgtgtat cacggattgc ggatggatga
    32221 cgacttcgag tttgtcgggc ggacagttga acagccaatg cgcttcaatc agctccaaac
    32281 ctttgttcat catggtggcg gaatcgacgg agattttgcg tcccatacgc caattggggt
    32341 gtttgaccgc ttggtcgggc gtaatgctgt cgaacgtgtt taaatcggtc gttaaaaatg
    32401 ggccgccgga agcggtcagg ataatcgaac ggatgccgtg ttcgttcaga cgacctgtgt
    32461 aatcgcgcgg caaaacttgg aaaacggcgt tgtgttcgct gtcgacgggt aacactgccg
    32521 cgctgtttgc acgggcggtt tccataaaca acgcgccgga aaccaccaac gtctctttgt
    32581 tcgccagata aatagttttg cctttttgcg ccgctgcgag cgcggaaggc agccccgccg
    32641 ccccgacgat ggcgcacatg acaccgccga cttcgtcggc agacgcaacg tcaaccaatg
    32701 cctgcgcgcc gtgtaaaacc tgtgtcgccg tgccgtcgtg tttcaacagg gcttcaagcc
    32761 gggcggcgtg ttccgtatcg gcaacgacgg catattcggg gcggaacgtt tgacattgag
    32821 ccgccagttt ctcgacctgc ttatgccctg ccagcgcgaa tacgcggaat ttttcggggt
    32881 ggcgggagac aacgtccagc gtgctttcgc ctatgctgcc ggtactgcct aatatggtca
    32941 ggacttgtgg tgtcataatg gggataactt tataccggat gccgtctgaa gcgttttcag
    33001 acggcataga atcaatttaa aaccgacatc atcgccgcat agacgctgat aacggcaatc
    33061 aggctgtcgg tacggtcgaa cacgccgccg tgtccgggca gcaggttgct gctgtctttg
    33121 atgcctgccg cgcgcttgag ccagctttcc aaaaggtcgc cgcatacgct gacgacggtc
    33181 agcaccaaac cgattaacac ggtatcgaac cagcctgtat cgaatgccag ccagccggca
    33241 cttcgtacgg cggtcatgta cacggccacg caaaccgcgc cgccgattgc gccttcccag
    33301 cttttgccgg ggctgattgc cggtgcgatt ttgtgtttgc cgaacgcctt gccgctgaaa
    33361 tacgcgaaaa catcggcaac ccacaccaaa cccatcacgg cgagcagcgg cagggcatca
    33421 tcagggtgcg ggcgcaggga tacgagcgcg aaccaaaacg gcatgaccag aagccaaccg
    33481 acggcataaa cctgccaacc gccgttgagc ctccatttga atctcaacca caaaggcatg
    33541 atggcgagcc aaaatgccaa aacaacatac caaaccaaat taggcagcat ccagccgccc
    33601 gcataggcaa ccacgccgaa aaccaaggtt gcggcgaggt aatggttggt tttaattttg
    33661 cacaaaccgc tcatacgggc atattcccac aaggcaatca gggcaatcag tccgcaaaat
    33721 gcagcccaca accattgcgg cgcgtaaaac agcatgccca gcatcagcgg cagcagccac
    33781 atagcagtta tcacccgttg tttcagcata ttcagttcct ttgctgttcg ataggcagtt
    33841 gctcggaggt gcgtccgaac cgccgttcgc gtttttggaa cgaagcgacg gcatcgtcca
    33901 aagccttgcc gtcaaaatcg ggccacaaaa tatcggtgaa atacagttct gcatatgcca
    33961 tctgccagag caggaaattg ctgatgcgcg tttcgccgcc ggtgcggatg aacaaatccg
    34021 gttccggtgc atcgcccagc atcaagtgtt tcgccagcgt gtcttccgta atctcggata
    34081 cgccttcggc aatcagtttg tttgccgcct gcaaaatatc ccagcggccg ccgtaatcgg
    34141 cggcaatgct cagggtcagg ccggtattgt ttgccgtcaa cgcttccgcc tcttcgatgc
    34201 cttgcagaat ctgccggttg aagcgttcgc ggctgcccaa tatcttcagg cgcatattgt
    34261 tttcgtgcag gcgacgtacc tgtttttgca aagcctgtaa aaacagcccc atcaggaacg
    34321 aaacttcgtc ttcggggcgg cgccagtttt cggttgaaaa ggcaaacacg gtcagatatt
    34381 gcacacccag tttggcgcaa tgcttcacca tattttccaa tgcgtccaaa ccgcgtttgt
    34441 gtcccattat gcgcgggagg aaacgttttt tcgcccaacg gccgttgccg tccataatca
    34501 cggcgatatg cttgggaatg gcggtgtgtt ccaaaacggc ctgcgtgctg cttttcatgt
    34561 ctgcctttcg cggttcggca ttcaaatgcc gtctgaacgc cgaaccgtgc aggttaaatt
    34621 gccatcaaat cttcttcttt ggcagtcagg agtttgtcgg cttcggtaat gtatttgtcg
    34681 gtcagttttt gaaccgcttc ttcgccgcga cgtgcctcgt cttcggaaat ttctttgtct
    34741 ttgaggagtt ttttgatgtg gtcgttggca tcgcggcgca cgttgcggat ggagacgcgg
    34801 ccttcttccg cttcgccgcg tacgacttta atcaggtctt tgcggcgttc ttcggtcagc
    34861 atgggcatcg gcacgcggat caggtcgccg acagctgccg ggttcagccc caagtttgaa
    34921 tcgcggatgg ctttctcgac tttgaccgcc atattgccct caaacggttt cacgccgatg
    34981 gtgcgcgcgt ccagaagcgt tacgttggca acttggctga cggggaccat gctgccccag
    35041 tattcgactt ccacttggtc gagcaggccg gtatgcgcgc ggccggtacg cactttcgcc
    35101 agattttctt tcagtacttc gaccgaacgc tgcattttgc cttcggctgt tttttgaata
    35161 tcgttgatca tattgttctt tcggtgggat aaggtgggcg ggagaccgtc tgaacgcgtt
    35221 tcaagccgtt cagacggcat aaagaccgtt aaccgcgaat agtaccgtta ttcgggcata
    35281 acgacaaggt aggcggattg gggatgccgt ctgaagcgac aggcgtttca gacggcatcg
    35341 tgtccgaccg tcagccgtgt tcccgtgttt caagcaggct ttggcgcagg tgttggcgtt
    35401 cgtgggcatc cagccatttg cggcgggtgc gttgcagcag gatgacgagg gcggaaattt
    35461 cctgacgcat attggtgctg agccagagga agccctgcca ttggtagtgg aggtgttcgg
    35521 cgagggcttc cagttcgggg ttgatagcgg tgtcgatgcg gatgcggcgg gcgtgtctgc
    35581 cgttgataag ggcgacggtt tgttgcaggt cggtttggag cagtgtgaag tggcggtcga
    35641 gcagccggat ttcgctgccg ttgagtttgg gagattgcag cttggcggca gtggtcagga
    35701 gcagctcggt ggtgttgaca attttacggt gggcgtgctg catggcttcc atcatggcgg
    35761 ggctgatgcg gctttcgccc gatgtggcgg cgaggtggct gcggcttttg accatgcgtg
    35821 cgttgatttg gcgcattttc gccatgttct cttcgaggcg ttcgcgggtc atgcgcctgc
    35881 cgttgctgat ttcggcaatc attttgctgc agtcggtcag gttgtcggca agcatgaaac
    35941 gccacatcag tgtggatttc agcggcagca gtttggcggc ggcgatggcg atggccgcgc
    36001 cgatgaggac gttcatcgcg cgcatgagtc cgctgtcgag ccattcgctg ccgttgtcgc
    36061 cgatgagcat gcacatcgtc agccccgcca gcatggggac gtaaccgttt ttgccgaccg
    36121 ccgcccaacc ggccagtgcg cttgccgtgc cgacggtgag gtagaagagg agattgtcgt
    36181 ggaaataatg ctggttcagc cataaaacgc ccaaacccgc gcccagcccg atgaccgtac
    36241 cgagcatacg ttccaccgcc ttggagtaaa tcgccccttg aaactggagc atgccgagga
    36301 cgacgaagac ggtcatgcct atccattcgc cgtgttggag gtggaacagc cgggcgagtg
    36361 cggtggcgaa caggacggct ccgccaagcc ggacggagtg gatgaggcgg cggtagcggt
    36421 agcgttcgta ggagttgagc cagcggctgg aaaggcggtt gcgttgcgag gtgttcatat
    36481 cggttgccgt ctgaagcgga aatgtgaaaa agcacaggct tcctgggata gggagggtct
    36541 gtgcttggta ttggtgccgg agaagggaat cgaacccccg accttcgcgt tacgaatgcg
    36601 ctgctctacc gactgagcta caccggcttt tttcgttatg atatatatga acggttgttt
    36661 ctgcaacttt tcgggctgct ggcaaagttt tgcacggtat aatgcggttt gcttcgtgag
    36721 gagagtcggt gatgtatgtg aacgagaaat atccctatgc ggctttgttt gcggggctgg
    36781 tgtttttgac gctgccgttc gcactgtcgg tgcatgatgc cttggcattt gcgttcggac
    36841 ggacggggct gctggtgtcg gtgtcggacg gcggattcgg ctggcgtggc ggttgggaca
    36901 gcactgtttg gtttgtgttc ggtgtgtttg cgtttttgaa tgtggttgtg tcggcgggtc
    36961 tgacgaaact ggcgtacaaa aagatgatgc ggcggcattc gcgttacgcg ctgtttctgt
    37021 caggcgtggc ggcttgcgcg gcggcggcgg tggcttggat tttcgaactg ttgttgggca
    37081 gtgccgcctt gggcgggctg cgcggggagg cggtgttgga atatgcgttt gccgtgtggc
    37141 tggtggcgat gctgacgctg cccaaacgct tgacgcgcgc gccagtgcag ctggtggtgt
    37201 tccacaggaa aaaataggac ggaacgggaa atgccgtctg aaacccgaca cgcggtttca
    37261 gacggcatgt ttttccgcta acattacgcc tgaatatgga taggaagcag atatggaacg
    37321 taaagaacgc ctacaatcgg gcattgctgc gatggggctg gatatttcgg aaacggcgca
    37381 ggacaggctt ttggcctatg tggatttgtt gaaaaagtgg aacaaaacct acaatctgac
    37441 cgccctgcgc gacgaggaaa aaatgattgt ccatcatctt ttggacagcc tgacgctgct
    37501 gccctatatc gaaggcgcac agacgatgct ggatgtcggt tcgggcggag gacagcccgg
    37561 cattccggcg gcggtgtgcc gtccggatgt gcagataacc cttttggatg cgaatacgaa
    37621 gaaaacggct tttttacagc aggcggttat cgagttggga ttggacaatg tgcgcgtggt
    37681 atccggacgc gtggaggcgg tttcggacgt gcgtgccgac gtgattacca gccgtgcgtt
    37741 tgcagaactg gcggattttg tgtcgtggac ggcgcatctg ttgaaagacg gcggctactg
    37801 ggcggcgatg aagggcgtgt atccgcaggg ggaaatcgac cacctgccgc aggatgtgtg
    37861 cgtcgaaaaa gtccaaaggc ttgacgtgcc gggcttggat gcggaacgcc atatcgtcat
    37921 actccgcaag cgttgagcgc acttcagacg gcatgaatac cttttttgtg cggataaagg
    37981 taaaattccg cactgttttt cttttttcaa catcagacgg gacacgggcg ggacatgagt
    38041 gcgaacatcc ttgccatcgc caatcagaag ggcggtgtgg gcaaaacgac gacgacggta
    38101 aatttggcgg cttcgctggc atcgcgcggc aaacgcgtgc tggtggttga tttggatccg
    38161 cagggcaatg cgacgacggg cagcggtatc gacaaggcgg gtttgcagtc cggcgtttat
    38221 caggtcttat tgggcgatgc ggacgtgaaa tcggcgacgg tacgcagcaa agagggcgga
    38281 tacgctgtgt tgggtgcgaa ccgcgcgctg gccggtgcgg aaatcgagct ggtgcaggaa
    38341 atcgcccggg aagtgcgttt gaaaaacgca ctcaaggcag tggcggaaga ttacgacttt
    38401 atcctgatcg actgtccgcc ttcgctgacg ctgttgacgc ttaacggctt ggtggcggcg
    38461 ggcggcgtga ttgtgccgat gttgtgcgaa tattacgcgc tggaagggat ttccgatttg
    38521 attgcaaccg tgcgcaaaat ccgtcaggcg gtcaatcccg atttggacat cacgggcatc
    38581 gtgcgcacga tgtacgacag ccgcagcagg ctggttgccg aagtcagcga acagttgcgc
    38641 agccatttcg gggatttgct ttttgaaacc gccatcccgc gcaatatccg tcttgcggaa
    38701 gccccgagcc acggtatgcc ggttatggct tacgacgcgc aggcaaaggg taccaaggcg
    38761 tatcttgcct tggcggacga gctgatggcg agggtgtcgg ggaaataggt caatccaaat
    38821 cgggctgccc gtgcctttat gctgtttggc cgagtgcgtt aatatggcgg attaaaataa
    38881 aaatacttat atcg
//
LOCUS       DAXHTY010000005        38382 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXHTY010000005 DAXHTY010000000
VERSION     DAXHTY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.005"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            320..754
                     /locus_tag="ACFNMK_000236"
     CDS             320..754
                     /locus_tag="ACFNMK_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC6363582.1"
                     /translation="MNFALSVITFTLASFLPVPPAEAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(803..2146)
                     /locus_tag="ACFNMK_000237"
     CDS             complement(803..2146)
                     /locus_tag="ACFNMK_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC6363583.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(2151..2804)
                     /locus_tag="ACFNMK_000238"
     CDS             complement(2151..2804)
                     /locus_tag="ACFNMK_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC6363584.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2955..3245
                     /gene="gatC"
                     /locus_tag="ACFNMK_000239"
     CDS             2955..3245
                     /gene="gatC"
                     /locus_tag="ACFNMK_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC6363585.1"
                     /translation="MALTLADVDKIARLSRLHLTAAEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3308..4753
                     /gene="gatA"
                     /locus_tag="ACFNMK_000240"
     CDS             3308..4753
                     /gene="gatA"
                     /locus_tag="ACFNMK_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC6363586.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4750..5682
                     /locus_tag="ACFNMK_000241"
     CDS             4750..5682
                     /locus_tag="ACFNMK_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC6363587.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5725..7155
                     /gene="gatB"
                     /locus_tag="ACFNMK_000242"
     CDS             5725..7155
                     /gene="gatB"
                     /locus_tag="ACFNMK_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC6363588.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEVEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAATLNKAGLELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7247..8257
                     /locus_tag="ACFNMK_000243"
     CDS             7247..8257
                     /locus_tag="ACFNMK_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC6363589.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQIGGHSEQALSEAEKAQGKILMCCTTAQSDISLNIPGYKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLLGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSGRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8386..9018)
                     /gene="pdxH"
                     /locus_tag="ACFNMK_000244"
     CDS             complement(8386..9018)
                     /gene="pdxH"
                     /locus_tag="ACFNMK_000244"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC6363590.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLSEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLLEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9249..10298
                     /locus_tag="ACFNMK_000245"
     CDS             9249..10298
                     /locus_tag="ACFNMK_000245"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6363591.1"
                     /translation="MSKQPTSKRQWRDGVAPSAKKTAKPFKSKARPKDETGKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGESANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(10367..11908)
                     /locus_tag="ACFNMK_000246"
     CDS             complement(10367..11908)
                     /locus_tag="ACFNMK_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC6363592.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVSTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVVETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQASVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMIFVKA
                     LDLFS"
     gene            complement(12519..13874)
                     /gene="xseA"
                     /locus_tag="ACFNMK_000247"
     CDS             complement(12519..13874)
                     /gene="xseA"
                     /locus_tag="ACFNMK_000247"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC6363593.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPARPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALSHAFHQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            14071..14892
                     /gene="nadE"
                     /locus_tag="ACFNMK_000248"
     CDS             14071..14892
                     /gene="nadE"
                     /locus_tag="ACFNMK_000248"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC6363594.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(14962..15435)
                     /gene="dnrN"
                     /locus_tag="ACFNMK_000249"
     CDS             complement(14962..15435)
                     /gene="dnrN"
                     /locus_tag="ACFNMK_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC6363595.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPIGVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            15733..16065
                     /gene="trxA"
                     /locus_tag="ACFNMK_000250"
     CDS             15733..16065
                     /gene="trxA"
                     /locus_tag="ACFNMK_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC6363596.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(16118..17065)
                     /locus_tag="ACFNMK_000251"
     CDS             complement(16118..17065)
                     /locus_tag="ACFNMK_000251"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6363597.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAV
                     DVYGDQIHLQEYDTGWLMQPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGEDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            17393..18781
                     /locus_tag="ACFNMK_000252"
     CDS             17393..18781
                     /locus_tag="ACFNMK_000252"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6363598.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPSALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDADIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYIGRKLPELTIEGMEPTRKRKPAGGKPKG
                     KGGWGDRKAGGQRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            complement(19209..19409)
                     /locus_tag="ACFNMK_000253"
     CDS             complement(19209..19409)
                     /locus_tag="ACFNMK_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363599.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(19423..19977)
                     /locus_tag="ACFNMK_000254"
     CDS             complement(19423..19977)
                     /locus_tag="ACFNMK_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363600.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            complement(20062..20562)
                     /locus_tag="ACFNMK_000255"
     CDS             complement(20062..20562)
                     /locus_tag="ACFNMK_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363601.1"
                     /translation="MNAMPQTAAQNETLHICSIIVDDARDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            20966..22159
                     /locus_tag="ACFNMK_000256"
     CDS             20966..22159
                     /locus_tag="ACFNMK_000256"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinyldiaminopimelate
                     transaminase"
                     /protein_id="HFC6363602.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALADALNTQMHKLWHISNIYTTRPAQELAQKLVKNSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATETIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVCSQGQKLQTALRSLGEQTGVFKQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(22224..23468)
                     /gene="clpX"
                     /locus_tag="ACFNMK_000257"
     CDS             complement(22224..23468)
                     /gene="clpX"
                     /locus_tag="ACFNMK_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC6363603.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTSSESVGQTSEEPGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGKEPELVFES"
     gene            23658..24029
                     /gene="rbfA"
                     /locus_tag="ACFNMK_000258"
     CDS             23658..24029
                     /gene="rbfA"
                     /locus_tag="ACFNMK_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC6363604.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTVLNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGMNLSALIDQVAAEKPVED"
     gene            24062..24985
                     /gene="truB"
                     /locus_tag="ACFNMK_000259"
     CDS             24062..24985
                     /gene="truB"
                     /locus_tag="ACFNMK_000259"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC6363605.1"
                     /translation="MNTGTPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIVATARAD
                     ISLAEFQTACKALTGDIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(25027..25137)
                     /locus_tag="ACFNMK_000260"
                     /pseudo
     CDS             complement(25027..25137)
                     /locus_tag="ACFNMK_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            25307..25771
                     /locus_tag="ACFNMK_000261"
     CDS             25307..25771
                     /locus_tag="ACFNMK_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6363606.1"
                     /translation="MKTDIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDLRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRSNSQPASQPASQPANYTLN"
     gene            26005..27462
                     /locus_tag="ACFNMK_000262"
     CDS             26005..27462
                     /locus_tag="ACFNMK_000262"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6363607.1"
                     /translation="MIYIDPPYYFVKKKQQDSFGYNTNFKLSTWLTFMKNRLSIAKEL
                     LTDDGIIVISIDDDGNAYLKILLDEIFGFENFIGNLPTIMNLKGNNDEYAFAGTHEYT
                     LVFAKNKDKSTFYEFPIDEDNFLEKWEEDEIGFYKKGAPMRATGTEDKREDRPEMFYP
                     FLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQKLKEKYENLGYNFILPIADKQWGRW
                     RWGYSIKNKARLQTDIIVSQSKNGISLYKKQRPELDDLPTKKPKTIFYKPEYSSGNGT
                     EQMKNLFGEKAFKNPKPEELIQDFITITTNESDIVLDYHLGSGTTAAVAHKMNRQYIG
                     IEQMDYIETLAVERLKKVIDGEQGGISKAVNWQGGGEFVYAELAPFNETAKQQILACE
                     DSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQEPEFQSLALDEQKQMVLEMLDLNQMY
                     VSLSEMDDEQFAGCLNDDDKALSRAFYQTEKKDGE"
     gene            27452..30190
                     /locus_tag="ACFNMK_000263"
     CDS             27452..30190
                     /locus_tag="ACFNMK_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC6363608.1"
                     /translation="MANNKMLFEVIENRKAVYLEDGDDEKCRLPEFVERNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIGSTHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAPTKSKKRDAEIEHKGWEH
                     MVLELLLNKNGNPSENVLLEFTATPPEKADVQQKYADKIITKFGLKEFLQKGYTKEIN
                     LVSSTLSKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKTVDESKADYLAFLNW
                     VENVQVDDFSFLKTFSTSLNDSDNANEQGKTRTEQALKFMQDNKFGFAHLANWVKQNY
                     QKHNIIITNSENNKTKTEKTDSETEKLLNNLEAADNPIRAIFTVGRLTEGWDVLNLFD
                     IVRLYEGQNGGGSNKKSCKTANTTVSEKQLIGRGVRYFPFAFEGKQPNKRKFDNDMQH
                     ELRILEELFYYAHDEQSRYISELKNELRKDGYLPEKDDDKVLATFKLKSEFAANKDFR
                     ELLIWANKKIPNPNAKSNNADSLKANPQTLSFQVHGNQLLQETQFTADENDETARQIG
                     TQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLDIQNRNELQNNLLKD
                     WQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIGTKEFTPKKLWEIFGT
                     PKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARLGDLKSKYDVHLIRN
                     EEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFIEPKGEHLVETDGWK
                     EEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTESFPLGAASLEKMK"
     gene            complement(30334..31506)
                     /locus_tag="ACFNMK_000264"
     CDS             complement(30334..31506)
                     /locus_tag="ACFNMK_000264"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC6363609.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            31796..32242
                     /gene="iscR"
                     /locus_tag="ACFNMK_000265"
     CDS             31796..32242
                     /gene="iscR"
                     /locus_tag="ACFNMK_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC6363610.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            32271..33485
                     /locus_tag="ACFNMK_000266"
     CDS             32271..33485
                     /locus_tag="ACFNMK_000266"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC6363611.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            33551..33679
                     /locus_tag="ACFNMK_000267"
     CDS             33551..33679
                     /locus_tag="ACFNMK_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363612.1"
                     /translation="MNEQDLDLDNLDNLLEDFDGVTVEGGVDSENDDGCEGGACKI"
     gene            33745..34131
                     /gene="iscU"
                     /locus_tag="ACFNMK_000268"
     CDS             33745..34131
                     /gene="iscU"
                     /locus_tag="ACFNMK_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC6363613.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            34218..34538
                     /gene="iscA"
                     /locus_tag="ACFNMK_000269"
     CDS             34218..34538
                     /gene="iscA"
                     /locus_tag="ACFNMK_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC6363614.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(34498..34719)
                     /locus_tag="ACFNMK_000270"
     CDS             complement(34498..34719)
                     /locus_tag="ACFNMK_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC6363615.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            34801..35301
                     /gene="hscB"
                     /locus_tag="ACFNMK_000271"
     CDS             34801..35301
                     /gene="hscB"
                     /locus_tag="ACFNMK_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC6363616.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKKSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLLCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            35399..38149
                     /gene="gyrA"
                     /locus_tag="ACFNMK_000272"
     CDS             35399..38149
                     /gene="gyrA"
                     /locus_tag="ACFNMK_000272"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC6363617.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIRLIKESPNAVEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            38306..>38382
                     /locus_tag="ACFNMK_000273"
     CDS             38306..>38382
                     /locus_tag="ACFNMK_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6363618.1"
                     /translation="MRKSRLSQYKQNKLIELFVAGVTART"
BASE COUNT        10182 a        10420 c         9315 g         8465 t
ORIGIN      
        1 atttcagcca tcttcaataa attatagtgg attaacaaaa atcagtacgg cgttgcctcg
       61 ccttagctca aagagaacga ttctctaagg tgctgaagca ccaagtgaat cggttccgta
      121 ctatttgtac tttctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatattt
      181 ttgggaatct gttttacccc aatatataaa gcaccatatt aaggcggagt ttcttcccca
      241 ctttgaaccg aacccggaaa agacaccgcc caagccaatc ctgatgctgc cccggcagcc
      301 aaccatcaag gaaaccctaa tgaactttgc tttatccgtc atcacattta ccctcgcctc
      361 tttcctgccc gtcccgcccg ccgaagccgc cgtctttact tggaaggacg gcggcggcaa
      421 cagctattcg gatgtgccga aacagcttca tcccgaccag agccaaatcc tcaacctgcg
      481 gacgcgccaa accaaaccgg cggtcaaacc cgcccaagcc gacgcaggga agcgcacaga
      541 cggcgcggca caggaaaaca atcccgacac tgccgagaaa aaccggcagc ttgaggaaga
      601 aaagaaaaga attgccgaaa ccgaacggca gaacaaagaa gaaaactgcc ggatttcaaa
      661 aatgaacctg aaagcggtgg gaaattcaaa tgcaaaaaac aaggatgatt tgattcggaa
      721 atacaataac gccgtaaaca aatactgccg ttaatcggct ctagcgcaaa ccccatgccg
      781 tctgaaacgg cacggggttt gtttatttct gccagtaggt tttgacgttg acgaactcgt
      841 acagcccgaa ttcggacaat tcgcgcccgt aaccggaatc tttgactccg ccgaaaggca
      901 ggcgcaaatc gctgctggta tggcggttga taaacaccga tcccgcctgt attttttcgg
      961 caaaccgcca agcgcgttcg gtatcggcgg tataaatgca ggcacctagc ccgaacgggg
     1021 aatcattggc aagggcgatg gcatgttctt cgttttcggc gcgcaaaatc agggcggccg
     1081 gcccgaatac ttcttctctc cagacgcggc aggcaggatt taccctgtct aaaaccgtcg
     1141 cgggataaaa ccagcctcgc ccttgtggga tttttccgcc ggtcaggcat accgcgccgt
     1201 ttgaaacggc atcttcaacc tgcccgtgaa ccctgtcccg caaatcttcg cggtgcagcg
     1261 gtgcaagcgt agtatcggga tgtttggggt cgcccatttt caatttggcg cattcggcaa
     1321 gaaacagcgt gataaaacga tcggctgcgg cttcggttac gatgatgcgt ttggcggcgt
     1381 tgcacgattg ccccgcatcg cggaaacggg aataacaggc ttctgcggcg gcgcgttcca
     1441 aatcggcatc gggcatcacg ataaaggcgt tgctgccgcc gagttccaag acggttttct
     1501 tgaggtttgc gcccgcatgt gccgcaagga tgcgccccgt atgcgtcgag ccggtaaacg
     1561 ccatcgcatc ggtatcttca accgccttga gcgtgcccgc ctcatccagc cacacgcctg
     1621 ccagaggaat gccgtctgaa gccaaatcga acagtgcctg actgacgcgt gccacgctgg
     1681 gcgcgggttt gacggcgcac gcgttgcccg cgcacatagc gggaacggcg aaacgcaata
     1741 cctgccagac gggatagttc caaggcatga cggcaaacac cacgcccaaa ggctcgaagc
     1801 gcacctgact caaactcgcc tgcgtcgcga tggttttgtg ggcaagcagt tcgggggcaa
     1861 ggcgggcgta atagcgtatc agttcgatag acttgccgat ttccgcacgg cattcgtgca
     1921 agcagcgtcc gacttcctca cacaccattt ccgcaaaacg ctctttctcc gcctccaaac
     1981 ggtcggcaaa tttttgcagg cgcgcggcac gttcggttac gcccagttgc gcgaacgccc
     2041 cgccgcgcat tttcaaatcc gccagccgcc gttcaaactc cgcataatct tgagctggac
     2101 ggcggtaaag cgtttcgccc gtaaatacat tgacactgtg aaacatcgaa tcaacctgcc
     2161 agttgcggga atatcgtttt cagtcccgac acaataatct ccaccgatac cgccgccagc
     2221 atcataccca taatgcggtt taaaatcgtc agccccgtcg cgcccagcag gcggctgacc
     2281 ttcccggcaa cgattaaaat ggcataacaa atcgcactga ccaccaaacc ggccgcgata
     2341 atcaacgcga tgtcgccgta tgttttagcc gccgaagcgt aaataatcac ggtcgaaata
     2401 ccgcccgggc cgatggtgat cggtatggcg atgggcacga cggcaatcgc tccggcattg
     2461 cgggcggggc gcgcctgccc cgtttccggc tgcgcgccga gattctgctt ggcgggattg
     2521 tcgttgccgt tcatcataga aatggcaatc agcaacacca aaattccgcc gccgacctga
     2581 aacgaaccga cgctgatgcc caaaaccttc agcagcgtac cgccgatcag cgcaaatacc
     2641 gcaatcacgg caaacacggc aacggcggcc gtccgcgcga ccttcctgcg ctccttcgtg
     2701 ctgtgcccgt tggtcaggtc aaggtaaagc gacaacgcgc taaacggatt gatcagcacc
     2761 aaaaaagcca caatcagctt gccgatttcc atgcccaatc ccattatttc cccctccttc
     2821 aaacccgtgc ggcaggcatc cgatgctgca aattgccgcc gcaacggatt tttccgttat
     2881 aattaaaaat tcaagcaata cgccccatca tacccgaacg acggtatctt taccatcaga
     2941 caaggatgct tttcatggca ctgacacttg ccgacgtaga caaaatcgcc cgactctccc
     3001 gactgcacct gactgcggca gaaaaagaaa aatcgcttca agaattaaac gacattttca
     3061 ctatggtcga acagatgcaa accattaaca cagacggcat cgaaccgatg gcgcacccgc
     3121 acgaggccgc cctgcgcctg cgcgaagacg aagtaaccga aaccgaccgc gccgccgaat
     3181 atcaggcggg tgctccggaa gtacgcaacc gtctgtacat cgtaccgcaa gttatcgaag
     3241 aataatccga atatgcttca gacggcatca gcaataccgc ccgaagccct ttaaggatgg
     3301 aagatttatg acccaataca cattgaaaca ggcaagcgtc ctgttgcagt ccaaacagat
     3361 ttccgccgtc gaactggcaa gcgcatacct tgccgccatc gccgaaaaaa atcccgccct
     3421 caacggctat atcaccatcg accaagataa aacccttgca gaagcccgtg ccgccgacga
     3481 acgtatcgcg cagggcaacg cctccgcgct taccggcgta cccgtcgcct acaaggatat
     3541 tttctgccaa accggctggc gcagcgcgtg cgcttccaaa atgctcgaca acttcatctc
     3601 cccctacacc gccaccgtcg tccaaaacct gctcgacgaa ggtatggtaa cgctcggccg
     3661 caccaatatg gatgagttcg ctatgggttc gaccaatgaa aactcattct acggtgcagc
     3721 caaaaaccca tggaatcttg agcacgtccc cggcggttcg tcaggcggtt ccgccgccgt
     3781 cgttgccgcg cgcctcgccc ctgccgcgct cggttcggac accggcggct ctatccgcca
     3841 acccgcatcg cactgcggca ttaccggcat caaacccaca tacggcacgg tttcccgctt
     3901 cggtatggtc gcctacgcct ccagcttcga tcaaaccggc ccgatggcgc aaactgccga
     3961 agactgcgcg attctgttaa acgcgatggc aggtttcgac cccaaagact ccaccagcct
     4021 cgagcgcgaa aaagaagact acacccgcga tttgaaccaa ccgctcaaag gtttgaaaat
     4081 cggcctgccc aaagaatatt tcggcgaagg caacagcgcc gatgttctga cggcattgca
     4141 aaacaccatt gatttgctga aagcccaagg cgcggaattg attgaagttt ccctgccgca
     4201 aaccaagctg tccatccccg cctactacgt cctcgcctcc gcagaagcca gcaccaacct
     4261 ttcacgttac gacggcgtac gttacggaca ccgtgccgcc caattcgccg atttggaaga
     4321 aatgtacggc aaaacccgcg ccgaaggttt cggcagcgaa gtcaaacgcc gcatcatgat
     4381 cggcacttat gtactgtcgc acggctacta cgatgcctac tatctcaaag cccaaaaact
     4441 gcgccgcctc gttgccgatg attttcagac ggcatttgca cggtgcgacc tcatcctcgc
     4501 gccgaccgca cccactgcag ccccaaaaat cggagcggat gcttcgccgg ttgaaaccta
     4561 cttgagcgat atctacacca tcgccgtcaa cctcgccgga ctgcccgcat tgaccctgcc
     4621 cgcaggcttc agcggcggcg gactgcccgt cggcgttcag cttgtcggca actacttcgc
     4681 cgaagccaaa atcctcggtg cggcgcatca aatccaactc aacagcgatt ggcacggcaa
     4741 acgacccgaa tgaagcagaa ccgcaccttt accttccccg attttcgcac cgtttacagc
     4801 tatgcgcctt tatatcggct gcaacattta aaatacacat tgcgaaaatt tttcggaaaa
     4861 aaagaaattt acgccttcga gcagtttgtc aacgcctccc ctatccgtca ggggctgttc
     4921 ctccactgcc cgcaaaatgc ctatccgctg ctgcgcgaat ttgttgacag gcgttttaac
     4981 tgcaaacgcc gtttagatgc gatgacggca gattttctca tggcggaaaa actgttcggc
     5041 acagacatcc tgcaccaaat ggaagactac cgcttccatt tggtcttggc gcacctttca
     5101 gacggcatca gcttgtggct caaccgcaac gacaactgcg tcgaagaagg cgcgtggtct
     5161 ttatctttgc gcgacgaagc aggcaaccgg ctgtatatgg cgactttcgc ctttgtcggc
     5221 acacacctgc tgacagcctc cgtacaaggg cctgcgggtg aagaagccaa agacactgtc
     5281 cgccgcataa ccaaacaact tcacggcttg cgtccccaac aactgatggt aaccgccctg
     5341 caatatttcg ccgccgtact cggcttggac ggcgcaatgg gcattgcaca aaaacatcag
     5401 gtcaaactgc gctggaaact taaaaagcgt gtcaaaatga attacgacgc attctggcag
     5461 gaatacggcg caagtttgga acgggacggc tactggcatc tcccccaaac ccccgcccgc
     5521 aaagaccttg ccgacatcga aagcaaaaaa cgctcgatgt accgcaagcg ttatgaaatg
     5581 ctggacaaca tggttgcaga gatgaaggac ggtctgaaaa cagaagcacg cggcatttca
     5641 gacggcatcc aaacggaaaa accgctccgc cggacagcct gacgcgaaga ctatcgaatt
     5701 gatattttag agaaagaagc tcttatgacc tgggaaaccg taatcggctt ggaaatccac
     5761 gtccaattga acaccaaatc caaaatcttc agcggcgcat cgaccgcatt cggcgcagaa
     5821 cccaacgcgc acgccggcgt ggtggaatgt gctttgccgg gcgtactgcc ggtaatgaac
     5881 cgtgaagtcg ttgaaaaagc catcaaatta ggcttggctt tggatgcgaa aatcaaccgg
     5941 aaaaacgtgt tcgaccgtaa aaactacttc tatcccgact taccaaaagg ctatcaaatc
     6001 agccagttgg acttaccgat tgtcgaacac ggcaaattgg aaatcgtagt cggcgacgat
     6061 gtgaaaacca tcaacgtaac ccgcgcccat atggaagaag acgcgggcaa gtccgtacat
     6121 gaaggcttga acggcgcgac cggtatcgac ctgaaccgcg ccggcacgcc tttattggaa
     6181 gtggtgtccg aacccgaaat gcgctccgcc gccgaagccg ttgcctacgc caaagcctta
     6241 cacagcttag taacctggct ggacatttgc gacggcaata tggcggaagg ttcattccgc
     6301 gtcgacgcaa acgtatccgt gcgtccgaaa ggtcaagcag agttcggcac gcgccgcgag
     6361 attaaaaacc tcaattcctt ccgctttttg gagcaggcaa ttaattacga agtggaagcg
     6421 caaatcgaga ttttggaaga cggcggcaaa gtacagcagg caaccatgct gttcgaccct
     6481 gaaaaaggtg aaacccgcgt gatgcgcttg aaagaagacg cgcacgacta ccgctacttc
     6541 cccgaccctg atttgctgcc cgtcatcatt tcagacgccc aaatgcaaaa agccaaagca
     6601 gaaatgcccg agctgccgaa agaaatggca gcgcgtttcg tggcggatta cggcgtgtcc
     6661 gaatacgacg cgcgcctgct caccgcaagc cgcgcacagg ccgcctattt tgaagaagcc
     6721 gccaaagaga gcggacaagg caagccgact gccaactgga tgaacggcga actcgccgcc
     6781 acgctgaaca aagccggcct ggaacttgcc gacagcccga ttaccgcccc gcgcctcgcc
     6841 gcgctggtag gcaaaatcgc cgacggcaca ttaagcggca aactggcgaa aaaagccttt
     6901 gaagccatgt gggcagaacc cgaagccacc attgccgaaa tcatcgaaaa acacggtttg
     6961 caacagatga ccgataccgg cgcggttgaa gcgatggtgg acgaagtgct agcaaacaac
     7021 gccaaagccg tggaacagtt taaatccggc aacgaaaaag ccctgaatgc gattgtggga
     7081 caagtgatga aagccagcaa aggcaaagcc aaccccgcgc aagttcaaga actgattaaa
     7141 gccaaacttg cctgataagc aagatgccgt ctgaaaccca gatccgacgt tcagacggcg
     7201 ttttcacagt ctgtccacat taaaacagca taaaaaacaa tattccatga accacaccgt
     7261 taccctgccc gaccaaacca cctttgccgc cggcgacggt gaaaccgttt tggccgctgc
     7321 cgcccgtcaa aacctcaatc tgccccattc ctgcaaaagc ggtgtctgcg ggcaatgcaa
     7381 agccgaactg gtcagcggcg atattcaaat aggcggacac tcggaacagg ctttatccga
     7441 agcagaaaaa gcgcaaggca agattttgat gtgctgcacc actgcgcaaa gcgacatcag
     7501 cctcaacatc cccggctaca aagccgatgc cctacccgtc cgcaccctgc ccgcacgcat
     7561 cgaaagtatg gttttcaaac acgatgtcgc cctcttgaaa cttgccctgc ccaaagcccc
     7621 gccgtttgcc ttctatgctg ggcaatacat cgatttactg ctgctgggca acgtcagccg
     7681 cagctactcc atcgccaatt cgcccgacca agaaggcatt ttggaactac acatccgcag
     7741 gcgcgaaaac ggtgtctgct cggaaatgat tttcggcagc gaacccaaag tcaaagaaaa
     7801 aggcatcgtc cgcgttaaag gcccgctcgg ttcgtttacc ctgcaggaag acagcggcaa
     7861 acccgtcatc ctgctggcaa ccggcacagg ctacgccccc atccgcagca tcctgctcga
     7921 ccttatccgc caagacagcg gccgcgccgt ccatttctac tggggcgcgc gtcatcaaga
     7981 tgatttgtat gccctcgaag aagcacaagg gttggcatgc cgtctgaaaa acgcctgctt
     8041 cacccccgta ttgtcccgcc ccggagaggg ctggcaggga agaaaagggc acgtacaaga
     8101 catcgcggca caagaccacc ccgacctgtc ggaatacgaa gtatttgcct gcggctctcc
     8161 ggccatgacc gaacaagcaa aaaatctgtt tgtgcaacag cataagctgc cggaaaactt
     8221 gtttttctcc gacgcattca cgccgtccgc atcataattc cccggtataa aaaagattca
     8281 ggctttccgt tcggaacaca aaaaacttcc cgtccgtatt ttccccataa aaaatgccgt
     8341 ctgaaaccca attccggctt tcagacggca tatgtttttt cctgttcaag gcgacagccg
     8401 ctcgcgtatc cagccaccat ccagcaaacg gtattggatg cggtcgtgca gcctgctcgg
     8461 cctgccctgc cagaactcaa gcaaatcggg aatcacaata tagccgcccc aatgcggcgg
     8521 acgcggcaca tgcagaggat gtttgagtcc gaccgccgcc gcctttgcca ccaataccgc
     8581 cttgttcgga atcacctcgc tctgcgcgct tgcccacgca cccaaacggc tctgatacgg
     8641 gcggctctca aaatattcgt ccgacaactt ctccgacagc ctttcaacac gcccttccac
     8701 gcgcacctga cgctccagct ccggccaaaa aaacgtcatc gccgcaaatg gatgagcatc
     8761 cagcgaacgt cccttacggc tgtgataatt cgtaaagaaa acaaaacctt cagaattaac
     8821 ttccttcagc agcaccatac ggctgttggg tctgccgcgt ccgtcgaccg ccgccacatt
     8881 aaccgccgtc ggctcgttga cctgtgcgcg taccgcctcg tccaaccacc gctcgaactg
     8941 ctcgatcgga ttgtcggcgc aatcgccttc cgacaattcc cgtttactgt aatcttcccg
     9001 aatattgtgc aaatccattt accgctcctc ttgtcatttg aaagattcta cccccgcacg
     9061 caaaccgatt tcaaccgttg cacaaacttt gcctcgcttc tccaaacctc aggacacaac
     9121 gatttcatcc gcaaaaccgc cgcataaggt acaatatcga accgtccgac cgagggcggc
     9181 attttatcaa cccgtcctgc cgcacacgcc gcagaagaac cgccttatca ggcgagttag
     9241 gaaaaatgat gtccaaacag cccaccagca aacgccaatg gcgcgacggc gtagccccgt
     9301 ctgccaagaa aaccgccaaa cctttcaaaa gcaaagcccg tcccaaagac gaaacgggca
     9361 aaaccgcatc ccaaccttac ggacaaaaag cttcagacgg catcaaacct caaaacctcc
     9421 ccaaacagcg cgccgccaaa gccaaaaaac tcgtcgtccg caatcccaac caaaaaatta
     9481 tggaacacgc gcgcgattta aaagaacgcc gcagcgacct atcgcgcatg gaacccgaac
     9541 gcctgcaaaa agtgcttgcc gcgtccggcg tcggctcgcg ccgcgaaatg gaagaatgga
     9601 tcaacaacgg ctgggtaacg gtcaacggca aaaccgcgca actgggcgac aaagtcaccc
     9661 ccgacgacca cgttaccgtc aaaggcagca tcatcaaact caaatgggcc gaccgcctgc
     9721 cgcgcatcat cctatattac aaacaagaag gcgaaatcgt ttcccgcgac gacccgcaag
     9781 gccgcgtcag catattcgac cgcctgccgc aggccgccag cagccgctgg gtcgccatcg
     9841 gacgcttgga catcaacacc agcggacttc tgattctcac cacctccggc gaactcgtcc
     9901 aacgtttcgc ccaccccagc ttcgaagtcg aacgcgaata cgccgtgcgc gtactgggcg
     9961 ggctgaccgg cgaacaaatg cgcgtcctca ccgaagaagg cgtgatgctc gaagacggct
    10021 tggcaaaagt cgaacgcatc cgcgaacaag gcggcgaaag cgcgaacaaa tggtacaacg
    10081 tcgtgattaa agaaggccgc aaccgcgaag tgcgccgcat ttttgaaagc caaggactca
    10141 ccgtcagccg cctcgtgcgc gtcggcttcg gtcccatcgg actgcccaac cgcctcaaac
    10201 gcgggcagtt ctacgaactc aaccccgccg aagtcgccaa catcatcaaa tgggcggaca
    10261 tgctgctgcc gggcgaacgc cgccgcaaaa aagcctaaac ccgccaaaac acaaaaatgc
    10321 cgtctgaaac atctactgtt tcagacggca ttttgttcgg gcgttttcag gagaaaaggt
    10381 cgagtgcttt gacaaagatc atcaccacgc cgtaggcgag cggtatgacg gccaatgccc
    10441 aacgccacca gaccgatatg ccgccgccgg aaactttttc gcccacaagg taagcgtcgg
    10501 atatggcggt ttcgtcatcg gggttgccgc tgtgtgcggc ggttttgatg tctttttcgt
    10561 ggtgtttttc gtgtacggat ttgacggcga ggttgcacaa caaaccgata atcagcaggc
    10621 acgccatgat gtacatggtt acgctgtatg cctgtgccgc cggtatgccg ctgtcgattt
    10681 ggctttggcg tatgtaattg accagtaccg ggccgatgac ggcggcggtt gaccaggcca
    10741 gcaggatgcg cccgtgaatc gcgccgacct gataggtgcc gaacaggtct ttcaggtagg
    10801 cgggaatggc ggcaaatccg ccgccgtaca tggaaataat cacgcaaaag ccgatgatga
    10861 acagggcttt gctgccgccc tcgccgatgg agggaacggc gaaatacagc agcgaaccga
    10921 gtacgaagaa aatggtgtag gtgtttttgc gtccgatttt gtcggaaacg ctcgaccata
    10981 aaaagcgtcc gcccatgtta aacaggctca ggaggctgac gaagcctgcc gccgcacctg
    11041 cgccgactga cgcctgcctg cctacggagg tttcggaaaa gagttcctga atcatcacgg
    11101 atgcctgacc caatacgcca atgccggcgg ttacgttcag gcacaatacc cagaacaaca
    11161 gccaaaattg cggcgttttc atggcttgtg acacgttgac atgattgctg ctgaccagct
    11221 tgttttgcgt tttcggcgcg gtatagcctt caggtttcca gccgtcggca ggtacgcgga
    11281 tggtaaacgc gccgaacatc atcagtgcga ggtaaagcag gcccaatacg gcgaaggttt
    11341 caacaacccc gactgaagcg gcgtttgaaa aggcgttcat cagtgatacg gaaagcggag
    11401 aggccagcat tgcgccgccg ccgaaaccca taatcgccaa accggtcgcc atacccggct
    11461 tgtcgggaaa ccatttcatc agtgtggaaa ccggcccgat gtagcctaag cctaagccta
    11521 cgccaccgat gacgccgttg cccaaataaa gcaggaagag gttgtgcgta ctcacgccga
    11581 atgcggatac gaagaagccc aggctgaaac agcaggcggc ggcaaatatg gctttgcgcg
    11641 gccctacccg ttccatccat gtgccgaaca gggcggccga cgcgcccagc atcgcgagtg
    11701 cgatactgaa aatccaacct acggtcgtca gcttccaatc tccggccgcc gattcggtta
    11761 tgccgataag tttggtcagt ggcgcgttga atacggaata ggcgtaaatc tgcccgatgg
    11821 caaggtgtac cgccaatgct gcgggcggta cgagccaacg gttgaaaccc ggcttggcaa
    11881 tgcttgcctc acggtctaaa aacttcataa catcctcttt ctgtcagttg aaaaataaaa
    11941 tttcatttgc tcaatggaaa cttattgaaa actataaaaa aatatcggat cggtttttta
    12001 tccgccccaa gatgccccgt ctgaaacatt tcgggtgtac ggaaaggttt ctgttttttc
    12061 cgacaaattc ctgcggcttt tcgcttccgg attcccgctt ttgcgggaat gacgaattaa
    12121 agattatctt aaggtcaagg gactggattc ccgctttcgc gggaatgacg acggcgggtt
    12181 gctgtttttt gtttataaat ttctgtaact ttggatttca gccatcttca ataaattatc
    12241 gaaacgtatc aatcgtcatt cccgcgcagg cgggaatcca tccagttcgt tcgatttcgg
    12301 ttttttaagt ttcgagtaac ttctgactcg tcattcccgc gtaggcggga atccggtttt
    12361 ttgagtttca gtcatttcta atacattccc accacttttc gcttccggat tcccgctttt
    12421 tcaggaatga cgaattaaag attatcttaa ggtcaaggga ctggattccc gctttcgcgg
    12481 gaatgacggc ggagggggag cggtttttcc gattgggttt aaatgcaatc gaacaaatcc
    12541 tgctgccctt gttctttgct tacgcgcacg tcggtttcgc cgtcggcgaa gataatgtgc
    12601 agcttctgcc cctgcttcaa aacatcggcg ttgcggatga cttgtccgcg tgtgtttttg
    12661 acgacggaaa agccgcgctc cagaatgtgc tgcggcgaaa cggcttcgag caatgctgct
    12721 tgggcggtca ggctttggcg gcggtgggta agcagttggt ggaaggcgtg cgacagggcc
    12781 gtctgaaggc ggacgatgtt gtttttgcaa acggaaacat caggacggta atgttgcagg
    12841 gcttgggttt ggcgttcgaa acgggcggtg tgggcacgga ggttttgctt catcgagtaa
    12901 gacagcgttt gcgccagctt gccgattgaa gcgcgctgct catcaagttt ttggcgcgga
    12961 tgacggattt gccgcgcaag ccagtcgagt ttttggctgg catcgaaata gcgttgttcc
    13021 aaaacggttt tcagacggcc ttgcgcctga gcgagacgat gtagcgattc ttggcggttg
    13081 gggctgacca gctccgccgc accggtcggc gtgggcgcgc gcacgtcggc gacaaaatcg
    13141 gcaagcgtaa aatcggtttc gtgtcccacg ccactgacga cgggaaccgt gcaggcttcg
    13201 attgcgcgca cgaccggttc ttcgttaaac gcccacaagt cttcaatgct gccgccgccg
    13261 cgacagacaa tcaacacgtc gcattcggcg cgttgcgagg cggttttaat cgcttgggca
    13321 atttgtaact cgctgcctgc gccttgaaca ggtgtcggat aaacgataac gggaatttcg
    13381 ggcgcgcggc gtttcaaggt ggtaacgaca tcgcgcaatg ccgccgccgc caaactggtt
    13441 acgatgccga tacattgcgg tcgggcaggc agaggttttt tgcgttccgc cgcaaacgcg
    13501 ccttccgcct gcaactgcgc cttcaaccgc tcataggctt cgtaaagctg ccccaaacct
    13561 ttgagccgta cttcgtttac ggtaatttga aattcgcccc gcgcctcgta aatactgatt
    13621 tttcctgata cctcgatatg atcgccttct ttcaaaggct tcgccaaacg cgccgccgca
    13681 cctttgaaca tcgcgcaacg cacctgcgcg cggctgtctt taagcgagaa gtaataatgt
    13741 ccgctggcgg cgcgggtcag gttggacact tcgccggcaa tccacaaacc ggcaaggtgg
    13801 ttttccaaaa tacttttggc aaatgcattc aactcggaaa cggacaacac gtcagaatga
    13861 aaaaaatcag acatcgaatc aatcaaataa taaaaaatat gaatatgttt tgaagcccaa
    13921 ggcggcatcg ggccgcctaa attgtcaaca atattataac acgcgccatc ttgccgcccg
    13981 ccttttcccg tatgactttt ttaagcgggg aatgggaaaa atattcatca acccgccctc
    14041 aatctattca aattacaccg ccgacaagct atgatgcgga tattttcgac aggaggaaaa
    14101 atggatacgc aggcagttat cacacatatt gtccgatggt tggacgaata cgccgcccgg
    14161 gcaaatgcaa aagggtttgt cgtgggcgtt tccggcggca tcgattccgc cgtcgtctcc
    14221 gcactcgccg cccgcaccgg ccgcaccacg ctgcttctgg atatgccgat acgccaacac
    14281 cccgaccagc ttgagcgggc aaggctgcac atccgcaatc tgcaacggca atatgccaat
    14341 gtaagcgcac aaacggtcga tctgaccgac accttccaga cctttgaaca aaccgtcggc
    14401 gttcatcaga cggcatttga caatcagccg ctttccctcg ccaacgccag aagccgcctg
    14461 cgtatgctga ccctgtacta ctacgggcag atacacggac tactggttac ggggacaggt
    14521 aacaaaattg aagatttcgg tgtaggattt tttaccaaat acggcgacgg cggcgtggac
    14581 atcagcccga ttgccgacct gaccaaaacc caggtttacc ggcttgccga agcattgggc
    14641 gtggacgagg cgattcaaaa agccccgccg accgacggcc tgtgggatac ggaacgcacc
    14701 gacgaagaac agatgggcgc aagctatccc gaactggagt gggcaatggg cgtgtacggc
    14761 acgcgcaaac ccgaagattt tgaagggcgg cagcgcgaag ttctggaaat ctatacgcgg
    14821 ctgcaccgcg cgatgcagca caaaatcaac ccgattcccg tgtgccgcat tccgcccgaa
    14881 ttgctgggct gaaacacgga aatgccgtct gaaacggaaa accgtatttc agacggcatg
    14941 gaaatatccg actcctatcc cttaagaatc gagtacgcgg gcaaacaaaa tatcgttttc
    15001 caaatgaatg tggtcgttca aatcctccac catttctttc gccagcgcgt aaagccgcgt
    15061 ccagcttccg caagcccctt ctggcggttg gaaattgtcg gtcagctctt tgagccgtgc
    15121 gatggcgcgg tcgtgttctt cgtgttcgtg catcatcacg ccgatgggca tcgccgcacc
    15181 gcgtccgaca ccctgattaa tcatcggaaa cagcatccgc tcctccttca tcatatgcat
    15241 cagcagttcg ttctgcatat aggcaagcag cccggcaatt tctgccggaa aggtatcggc
    15301 atggacttgg gccactttct gcgccagcgg caccaattct tcaaattgtg cgcggtggac
    15361 attgtggtag cgttgcagga tatgatcgac ggttgcacca aagggggcgg tctcccaaac
    15421 ggaaaaatca gtcatcgcag tgttcctttt acagggtttc gggtttggtt ttgaacattc
    15481 atactttaag aatcaattca aacggagcat acaccgcccg cgcgcttctg tacagcctca
    15541 aacgtattcc ttacattttg ataataaaag taattttcag aaataaaata ctgtccgaac
    15601 cgttttttag aatttgcaaa ggcgattggg gcggtacaga aaaactatta tcccgcccgc
    15661 ccacttgaaa tttttatgcc caagccctat cctgcacgct atcgtgccaa tcccaaccga
    15721 aaaggaaaaa taatgagcag cgaattaatt gtacacacca gcgatgccgc ttttgaaaaa
    15781 gacgttttaa atgcagatat ccccgtcctg ctggactttt gggctccgtg gtgcggcccc
    15841 tgcaaaatga ttgccccgat tttggacgac attgccgccg aatttgaagg ccgtctgaaa
    15901 gtggtcaaaa tcaacatcga cgacaacgaa gccaccccgt cccgtttcgg cgtgcgcggc
    15961 attccgaccc tgatggtgtt caaaaatggc gaagtcgtcg ccaccaaagt cggcgcattg
    16021 gcaaaaggtc agctgaccgc ctttgtcgaa gcctctatcg cctgataaag cgcaatcgaa
    16081 aaagccgccg gaagattccg gcggcttttt cgcaccctta agatttgtgg cggatttccc
    16141 agcacctatg gatttttttg ttgcggaaat cttcgggaac ggattgtttg gaaatgtctt
    16201 tgacggcgta ttgttccgat accaagtcgt ctaagacgaa gctgcgcagg ttgttggaaa
    16261 agtacaaaat gccgtctgaa gcgagcagct tcaccgcgcc gtcaatcagc tttttgtgat
    16321 cgcgctgaat gtcgaggatg tcggacattt tcttgctgtt ggaaaaactg ggcggatcca
    16381 tcacaatgag gtcgaactgc ctgccttccc catatgccgt ctgaagatat tggaacacgt
    16441 cggcgcggac gattttgtgc cgttccgtat cgatgccgtt caattcaaaa ttgcgtctcg
    16501 cccaatcgag ataagtgttc gacaaatcga cagtttcgct ggatgccgcg ccgccggtgg
    16561 cggcatagac ggtgaagctg ccagtgtagg aaaacaggtt taaaaaacgt ttgcccgccg
    16621 ccgtttcgcc gacttttttg cgcgtgtttc gatgatccaa aaaaagcccc gtatccaaat
    16681 acttatcaag gttaacccaa aacttgcggc cgttttcggt gatgacgaaa tcttcgcccg
    16741 ccttgccggt tttctcgtac tgctgcaaac ctttttggcg ttcgcggcgt ttgaggcgga
    16801 tttgttcggg cgcaaaaccg gtaacgaagg cgacggcttc caagacttcg gcaagccacg
    16861 cttcgtattc ttcgggctgc atcagccagc cggtatcgta ttcctgaagg tggatttggt
    16921 cgccgtaaac atcgacggcg aaggggaatt gtgggatgtc gcggtcgtaa atgcgccagg
    16981 cttcgatgcc gttgcgtttc gcccatttca taaggtgttt gatgtttttg cccaagcggt
    17041 tggcaaacgg tgtgatgtcg gtcattggtt tcaggcggaa taaagtggaa aacggcaatt
    17101 ttactgtaat taacgcccga ttgcttgacc gtttcgggca aaccctatac catccaccgc
    17161 ttatcttgtc atacgaagcc atcgccttcc aacctaaacc gcccttacgg gcgcgtttct
    17221 tctgttgctt tgattttaca aagcgtatct gtgcaggttg ccgtcgatgt aaaccacaag
    17281 caagccgctt gcgacaaccc tgtaacttca cattccccgt atcgttaccc ttccctgctt
    17341 caggccgtct gaacctttcg gacgcgggcg ttgttgtctt ccaaggatag ccatgtctat
    17401 taaatttgcc gatttgaacc ttgataaaaa catcttgtcc gccgtcagca gcgagggtta
    17461 cgaaagcccg accccgattc aggcgcaggc gattccgtcc gccttggaag gtcgcgacat
    17521 tatggcttcg gcgcaaaccg gctccggcaa aaccgccgcc tttctgttac cgactttgca
    17581 aaaactgacc aaacgcagcg aaaaaccggg caaaggcccg cgtgctttgg tgttgacccc
    17641 gacccgcgaa ctggcggctc aagtcgagaa aaacgcgctg gcgtatgcca aaaatatgcg
    17701 ttggttccgc accgtcagca tcgtcggcgg cgcgtctttc ggctaccaaa cccgtgccct
    17761 gagcaaaccg gtcgatctga ttgtcgccac gccgggccgt ctgatggacc tgatgcaaag
    17821 cggcaaagtt gattttgaac gtttggaagt gctgattttg gacgaagccg accgtatgtt
    17881 ggatatgggc tttatcgacg acatcgaaac catcgtggaa gcaacgccga gcgaccgtca
    17941 gactttgttg ttctccgcca cttgggacgg cgcggtcggc aaactggcgc gcaaactgac
    18001 caaagaccct gaaatcatcg aagtcgaacg cgtggacgat caaggcaaaa tcgaagaaca
    18061 actgctgtac tgcgacgata tgcgtcacaa aaaccgcctg ctcgaccaca tcctgcgcga
    18121 tgccgacatc gaccaatgcg tgattttcac gtccaccaaa gcgatgaccg aagtcatcgc
    18181 ggatgaattg tacgaaaaag gtttcgccgc caactgcctg cacggcgaca tgccgcaagg
    18241 ctggcgcaac cgcacgctga tggatttgcg caaaggccgc tgcaaaattt tggttgccac
    18301 cgacgttgcc gcacgcggta tcgacgtacc gaccattacc cacgttatca actacgacct
    18361 gccgaaacag gcggaagact acgttcaccg catcggccgt accggtcggg caggacgcac
    18421 gggtattgcg attacttttg ccgaagtgaa cgaatacgtc aaagtccaca aaatcgaaaa
    18481 atacatcggc cgcaaactgc ccgaactgac catcgaaggc atggaaccga cacgcaaacg
    18541 caaaccggcc ggcggcaaac cgaaaggcaa aggcggctgg ggcgatcgta aagccggcgg
    18601 ccagcgcggc gatcataaac cgggcaaaga aggcttcggc ggcaaaacgc gcggcgaagg
    18661 tttcaagaaa gaaggcttta agaaagacgg tttcaaaaaa accggcgaag gcttcaaagg
    18721 caaacgcaaa gccggcgatt cttttgcagg caaaagcgaa cgccgttaca aagaccgcta
    18781 agccccaacc tgcctcataa accaatgccg tctgaaaccg atttcgagtt tcagacggca
    18841 tttttgcaat gtttcaacac cgcccggctt tgatacccaa aggattaatc tgcaatatag
    18901 tggattaaca aaaaccagta ccgcgttgcc tcgccttggc tcaaagagaa cgattctcta
    18961 aggtgctgaa gcaccaagtg aatcggttcc gtactatttg tactgtctgc ggcttcgccg
    19021 ccttgtcctg atttttgtta atccactata aaaacccttt tccgctttgg caacgattga
    19081 aaatttccgt aaattcaaat atctagattc cttcctgcac gggaatgaca cggaagggtt
    19141 tcagatgcag ggtgggcatt cctgcccacc aaatcccgcc cttgcaacgg tgggcaagaa
    19201 tgctcgccct acggcttgac tgttcgatat gatgccgtct gaaaacccaa cggcggcatg
    19261 acaatgccgc cctgccaacg cacgtaaatc agaattgcca tcccgacatc aaacgcttgg
    19321 aaacaaaatg ccgtctgaaa atcaaacggc aacataacaa tgtccctaac aaatgcaaaa
    19381 atgccgtctg aaagctcttc agacggcatt ggtgcgctgg gtttaccgcc tcctgccgaa
    19441 accgcgcata gcggggcggc ggtaattggc gggcgggcgc gttgtcgggc ggtaacgctg
    19501 cgcctgcgcc gcctgttgtt ttgcacggag gctgcgcgtg ttcaaatccc tgctggtgcg
    19561 cgcattggga tgtgcggact gatggtaggc ggcacgcgcg cgccgggcga cgggactgtc
    19621 ttggttgcct gcccgtgtga atttgtttgc cagcgcgttg ccgataaacg cgcccgccgc
    19681 cgcgccgacc aggctttgca gcagccagct tcctgtcgat tggtcgtaaa tatactgctg
    19741 cccgtcttta ccggtaacgg gttgcccgtt gttgccgttt gcctgtgctt cggcaggaat
    19801 ggtgtctttg actgcttcgg gagtcagttg gtaaaccgta tcgtctgcct gctgtgcgag
    19861 ctgctgttgc agggcttcaa tctgtttctg ctgctgttcg agccgcgcct gcgtgtcgtc
    19921 ttggcaggcg gcgagtgcga atgttgcgat aagcgcggag gcgatgattt ttttcatgtg
    19981 tgtcctgttt gggtggaaaa tcggttttat tgtatcgccg tcgggaattt tggcaagcat
    20041 tctgccggca aatcgtgatg tttacagggg cagggtgtgc aatttgcgga caaatgcgag
    20101 gctgttggcg actgggttgc ctttgttttc gacttcgtgt tcggtttcta cggtcagcag
    20161 gcggcggttt ttgtgtttgt tcaggctcaa ttcggcttgt gcgggtgtga aaaacaggcg
    20221 gtgtttttcg gcaaagcggc gggcgcggct gttggcggtt ttcaaaatct gaagcagcga
    20281 tacgtccaga tggaagcgtc gcacgcccaa tacgagaatc cgtgcggcaa aaaaatcggc
    20341 ggcatcggta aactcggcgg ggctgctgcg gctgaagtcg tgccgttcgg cggctatcag
    20401 ggcggcaaca gtgcggattt gcggaatcat cgattcgctg atgagtgtgt cgtcccgccc
    20461 tgcatcgaga agcatggggg aaaaatcccg tgcatcatcg acaataatgc tgcaaatgtg
    20521 cagggtttcg ttttgtgcgg cggtttgggg cattgcattc atggtcattt tcctgattct
    20581 gtcgtgtgtt gccgaatcgg gcgacctgtg tgaaggtaac aaaaaagccg ccccattttc
    20641 gagcggcctg ttttgcgtat gggatggatt tcaagcaagc gcaaaaaagt accgcacgtc
    20701 tgtgtggtac caatagcaat aagcggttgt aaattttttg ccttgcatga tgaaatgccg
    20761 tctgaagata aaaatattgg ggagattcta aatcaaaacg ctgccgcgcc tcaagcattt
    20821 tatcgaaatt tttttgattt ttcatctatc cgattgaaaa tatttcggtt tatttttacc
    20881 gctgcccgat attgtcggca atttcccttt atctgctttg aaaaacggtg cataatcccg
    20941 agcaaaaccg caatcaggag caattatgca aaactatctg acccccaatt tcgcctttgc
    21001 accgatgatt cccgaacgcg cttcaggcag ccgcgtttgg gatacggaag ggcgtgaata
    21061 tattgacttt tcaggcggca tcgccgtcaa tgcgctggga cactgccacc ctgcccttgc
    21121 cgatgcttta aacacgcaga tgcacaagct gtggcacatt tccaatatct atacgacgcg
    21181 tccggcgcag gaattggcgc aaaaattggt gaagaacagt tttgccgaca aggttttttt
    21241 ctgcaactcg ggcgcggaag cgaatgaggc ggcgttgaag ctggcaagga aatatgcgcg
    21301 cgaccgtttc ggcggaggaa aaagcgaaat cgtcgcctgt atcaacagtt tccacggacg
    21361 cacgctgttt accgtgtccg tcggcggtca gccgaaatac agcaaggatt atgcacccct
    21421 gccgcaaggc attacgcacg ttccgtttaa cgatattgcc gcgctggaag ctgccgtcgg
    21481 cgaacagacc tgcgcggtca tcatcgagcc gatacagggc gaaagcggca tcctgcccgc
    21541 cactgcggaa tatttgcaaa ccgcgcgccg tctgtgcgac cggcacaatg cgttgttgat
    21601 tttggacgaa gttcaaaccg ggatggggca tacgggcagg ctgtttgcct atgaacatta
    21661 cggcgttgtt cccgatattt tgagttcggc aaaagccttg ggctgcggct ttccgatcgg
    21721 cgcgatgctg gcgacagaaa cgattgccgc cgccttccaa ccgggcacgc atggctcgac
    21781 tttcggcggc aacccgatgg cgtgtgcggt cggcagccgc gccttcgaca tcatcaacgc
    21841 gccggaaacc ttgcacaacg tctgcagtca ggggcagaaa cttcagacgg cattgcggtc
    21901 tttgggagaa caaacgggcg tgtttaagca agttcgcggc atggggctgc tgctcggctg
    21961 cgtgttggac gcgccttatc gcggacgcgc atccgaaata accgccgccg ccttgaaaca
    22021 cggcgtgatg attttggttg cgggtgcgga cgtattgcgt ttcgcgcctt cgctgctgtt
    22081 ggacgatgag gatacggcgg aaggtttgcg gcgtttggaa cacgtgctga cggaatttgc
    22141 cgccgcaaac cgcccctagg catcaaaaac aaaaaaccgc ttcggaaacg tggttcaacg
    22201 ttccgaagcg gttttgtttg ccatcaggac tcgaacacca attccggttc cttgccctct
    22261 tcgatgactg ccttaccgac caccactttt ttcaagcctt gcaaatcggg caggcggtac
    22321 atcgtatcca acaggcagcg ttcgacgatg gaacgcaggc cgcgcgcgcc ggttttgcgc
    22381 tccattgcct gacgcgcgat ggaacgcaat gcgccttctt caaactccag ttcgacattt
    22441 tccataccga acaaggcttg atactgcttg accaaagcat ttttcggctc ggtcaaaata
    22501 ttaatcagcg cgtcctcatc cagttcttct aacgttgcaa tcacaggcaa acgtccgatt
    22561 aattccggaa tcagaccgaa tttgattaaa tcttccggtt cgacgatgcc gaacagcttg
    22621 gtaatgtcgg cattttcgtc cttgctgtga acggacgcac cgaaaccgat accgcctttt
    22681 tcagtacgct ggcggatgac tttttccaag cctgcaaacg cgccgccgca gataaacagg
    22741 atgttggtgg tatcgacgtt gataaattcc tgattcggat gcttgcggcc gccttggggc
    22801 ggaacgctgg ccaccgtacc ttcaatcagt ttcagcaagg cttgctgcac gccttcgccg
    22861 gacacgtcgc gcgtgatgga cggattgtcg cttttgcgtg aaatcttatc gatttcgtcg
    22921 atatagacga tgccgcgttg ggctttttcg acatcgaaat cacatttgcc caagagtttg
    22981 gtgatgattt gctcgacatc ttcgccgaca taacctgctt cggtcagcgt ggttgcatct
    23041 gccatcacga acggcacatc cagtttgcgc gccaaagatt gcgccagcag ggttttaccc
    23101 gatccggtcg ggccgataag caggatgttg gatttcgaca attcgacatt agctccggct
    23161 ttcgggtggc gcaggcgttt gtaatggttg tacaccgaca ccgccaaggc tttcttggct
    23221 tgttcctgac cgataacgtg gtcgttgagg ttggcgacga tttcggcggg cgtgggcagc
    23281 ttgccgggct cttccgatgt ctgtccgaca ctttccgaag acgtgccgtc atttccgtct
    23341 tcatgcaata tttcaataca gtttgagacg cattcgtcac agataaaggc gttttcgccc
    23401 tcaattaaat gtttgacgtg tgatttggat tttccgcaaa aggaacaggt acggttttcg
    23461 ttggacatag ctttctttac aatgtatgcg ttacagaaac ggcacgcgcc gttcgggttg
    23521 ccaagtataa taactatatc cgttcttatc aatgtattac cttaaaatcc cgccgattcg
    23581 gctataatac gccctttcgc aaccgccccg gcggcaaaaa tgccgtctga aaccaaatct
    23641 gaaatctgag gatatttatg agaaaacccc aacgcggcta tgcccgccaa gaccgtgtca
    23701 aagaacaaat tatgcgcgag cttgccgaac tcgtccgtac cggactgaaa gacccgcgcg
    23761 ccggcttcat taccgtcaac gaagtcgaag ttacccgcga ttacagccac gccaccgtgt
    23821 tctacaccgt tttgaaccaa gatgcgcgcg aaattacgga agaagtgctg gaacacgcgc
    23881 gcggacacct ccgcagcgaa ttggccaaac gcatcaagct gttcaaaatc cccgagctgc
    23941 atttcaaata cgacgagtcc ttggaacgcg gtatgaacct gtccgccctt atcgaccaag
    24001 tggcggcgga aaaaccggtt gaagactgac ggatatgccc atgccgtccg aacatcgaac
    24061 catgaataca ggcacacccc aaaaacgtgc cgtcaacggt gttttgctct tggacaaacc
    24121 cgaaggcctt tccagcaaca ccgcgctgca aaaagcgcgg cgtttgtttc atgccgaaaa
    24181 agccggacat accggcgtgc tcgacccttt ggcaactgga cttttgcccg tctgcttcgg
    24241 tgaagcgacc aagttcgccc aatacctgct ggatgccgac aaagcctaca ccgccacgct
    24301 gaaactcggc gaagccagca gcacgggcga tgccgaaggc gaaatcgttg ccaccgcccg
    24361 cgccgatatt tccttagccg aatttcagac ggcctgcaaa gcattaacgg gcgacatccg
    24421 ccaagtgccg ccaatgtttt ccgcgctcaa gcacgaaggc aaaccgctgt acgaatacgc
    24481 ccgcaaaggc atcgtcatcg aacgcaaagc gcgctacatt accgtttacg ccatcgatat
    24541 tgccgaattt gacgcgccca aagccgtcat cgacgtacgt tgcagcaaag gcacctacat
    24601 ccgcaccctc agcgaagaca tcgccaaaca catcggcacg ttcgcccacc tgaccgccct
    24661 gcgccgcact gaaaccgccg gctttaccat cgcccaaagc cacacgcttg aggccttggc
    24721 aaatttgaac gaaacagaac gcgacagctt gctgctaccc tgcgacgtat tggtttcaca
    24781 ctttccccaa accgttttaa acgattatgc cgtccatatg ctccactgcg gacaacgtcc
    24841 gcgtttcgaa gaagacctgc cttccgacac gccggtacgc gtttacacgg aaaacggccg
    24901 ctttgtcggt ctggcggaat atcaaaaaga aatatgccgt ctgaaagcct tgcgcctgat
    24961 gaacacggcg gcatccgccg cctgaacggc ggttaaaaat acgggctgtg tttgaataat
    25021 gtgttgatat agtggattaa atttaaacca gtacggcgtt gcctcgcctt gccgtactat
    25081 ttgtactgtc tgcggcttcg tcgccttgtc ctgatttaaa tttaatccac tatatttccg
    25141 caaaattccg acacactcgg acacccgccc cgcttatcgc aactttgcga acgcccccgg
    25201 aaacagcaaa gacatcaaag gattgatttt attagaattt atttgcaaag ccatttgccg
    25261 ttacacaaga atggcacatt aaaatgactg atgaggattt ataacgatga agacagacat
    25321 tcaaaccgaa ttaacccaag ccctgctatc acacgaaaaa gtatgggcga acgaagaaaa
    25381 aaccatttta gccaaaaaca ttctgttgga tttggtggaa aaaaccgacc cgaccattat
    25441 cggtttgtta ttgaataatg atgagttaaa acgccatttc tttgtggaag tgaatggtgt
    25501 tttggtgttt aaattgcagg atttacgttt tttcttggac aaacacagcg tcaataattc
    25561 ctacacaaaa tacgccaacc gcattggttt gacggacggc aaccgctttt tgaaagacaa
    25621 ttcggatatt gtgttggatt ttccgtttaa agattgcgta ttgaatggcg gacaaagcac
    25681 cgaagaaggc gaagagattt attttaaacg cagtaatagc cagccagcca gccagccagc
    25741 cagccaacca gccaattata cactaaatta acccgaaaaa gacaagaaat cttttttaat
    25801 caaacgcttg cttttgatga aatcgatcgg ctttttgacg caaaagcatt ctcaaaattc
    25861 tctcgctata ccgcagacgg caaacaagcg gttggcgaaa tcaaacgtca ttcagacggc
    25921 acacccgccg aaaatctcat tatcaaaggc aataatctga tagccctaca ttcgcttgcc
    25981 aaacagttta aaggcaaagt gaagttgatt tatattgatc cgccttatta ttttgttaaa
    26041 aagaaacaac aagatagttt tggttataac actaacttta aattatctac ttggcttaca
    26101 tttatgaaaa atcgtttatc aattgccaaa gaattattaa ctgatgatgg gattattgtt
    26161 atttccattg atgatgacgg aaatgcttat ttaaagattt tgttagatga aatttttgga
    26221 tttgaaaatt ttatcggaaa tttacccact attatgaatt taaaaggtaa taatgatgaa
    26281 tatgcttttg caggcactca cgaatacaca ctagtctttg ctaaaaataa agataaatct
    26341 actttttatg aatttccaat agatgaagat aattttttag agaaatggga agaagatgaa
    26401 attgggtttt ataaaaaagg agctccaatg cgagcaacag gaaccgaaga taaaagagaa
    26461 gatagacctg aaatgttcta tccttttttg gtaaaaaata acacagttag tacaattaca
    26521 gatgaagaat tttctcaaat ctataataaa gatttagaag tattcaatga tgattttatt
    26581 caaaaactaa aagagaaata cgaaaatctt ggatacaatt ttattttacc tattgctgat
    26641 aaacaatggg ggcgttggcg ttgggggtat tcaataaaga ataaggcaag gctgcagacg
    26701 gatattattg ttagccaatc taaaaatgga atttccttgt ataaaaaaca aagacctgaa
    26761 ttagatgatt tacctacaaa aaaacctaaa actatttttt ataaacctga atacagtagt
    26821 ggtaatggaa ctgaacagat gaaaaatctt tttggtgaaa aggcatttaa aaatcctaaa
    26881 ccagaagaac ttatccaaga ttttattacc atcacaacca acgaatccga catcgtctta
    26941 gactaccatc ttggtagtgg cacaaccgcc gccgttgccc acaaaatgaa ccgacaatat
    27001 atcggtattg aacaaatgga ttatattgaa acgcttgccg ttgaacgctt gaaaaaagtg
    27061 attgatggcg agcaaggcgg tatttccaaa gccgtgaatt ggcaaggtgg tggcgagttt
    27121 gtttatgccg aacttgcccc atttaacgaa accgcaaaac aacaaatttt ggcttgcgaa
    27181 gattcagacg gcatcaaaac gctgtttgaa ggtttatgcg aacgctattt cttgaaatac
    27241 aacgtcagcg taaatgaatt tagtcaaatc attcaagagc ctgaatttca atccttggca
    27301 ttagacgaac aaaaacaaat ggtgcttgaa atgttggatt taaatcaaat gtatgtttca
    27361 ttatccgaaa tggatgatga gcaatttgca ggttgcttaa atgatgacga taaagcgtta
    27421 agccgtgcgt tctatcaaac ggagaaaaaa gatggcgaat aataaaatgt tgtttgaagt
    27481 gattgaaaac cgcaaagctg tgtatttgga agacggcgat gatgaaaaat gcaggctgcc
    27541 tgaatttgta gaacgcaatt taaaatatcc gttttttgaa tggcaaaaat cggctttaga
    27601 aaactttgtg atttttgacc gcacttcaaa attaaaggat tttcccgata tcaaaaaccg
    27661 ccctacccat ttgctgttca atatggcaac aggtgcaggc aaaacgatga tgatggcggc
    27721 gttgattttg tattattttg aaaagggtta tcggcatttt ctgtttttcg tcaatcaaaa
    27781 caatatcgta gataaaacgg aaaataattt tattggctca acgcacgcaa aatttttatt
    27841 taccgagaag attttgcaag gtgatacggt aattcctatt cgcaaagtgg aaatgtttag
    27901 cccacattca gacggcattg aaattaaatt taccagcatt caaaaactat ataacgatat
    27961 tcacaccgaa cgggaaaacc aaaccacatt ggcggatttg cataaattga accttgtgat
    28021 gctgggcgat gaagcgcacc atttaaacgc cccaactaaa agcaaaaaac gcgatgccga
    28081 aattgaacac aaaggctggg agcatatggt tttggaattg ttactcaata aaaatggcaa
    28141 tcccagcgaa aatgtgctgt tggaatttac cgccacgcca cctgaaaaag ctgacgtgca
    28201 acaaaaatac gctgataaaa tcatcacaaa atttggttta aaagaatttt tgcaaaaagg
    28261 ttataccaaa gaaatcaatt tggtatccag tacactgagc aagaaagagc gggtgttgca
    28321 cgctttattg tttgcttggt atcgccatca aatcgctttg aaatatggca ttgccaattt
    28381 caaacctgtg atgttgttta gaagtaagac ggttgatgaa tcaaaagcgg attatttggc
    28441 atttttaaat tgggtagaaa atgtgcaagt agatgatttt tcgtttttaa agacattttc
    28501 aacaagttta aacgatagcg ataacgccaa cgaacaaggc aaaacccgca ccgaacaagc
    28561 cctaaaattt atgcaggaca ataaatttgg gtttgcacat ttagcaaatt gggtaaaaca
    28621 aaattatcaa aaacacaata tcattattac caactccgaa aacaacaaaa ccaaaaccga
    28681 aaaaaccgac agcgaaacgg aaaaattgct aaacaatctg gaagcggctg ataatccaat
    28741 ccgtgccatt tttacggtgg gtagattaac cgaaggttgg gacgttttaa atttgtttga
    28801 tattgtgcgt ttgtatgaag ggcaaaacgg cggcggttca aataaaaaat catgcaaaac
    28861 ggcaaataca acggtatcag aaaaacagtt gattggtcga ggcgtgcgtt attttccatt
    28921 tgcgtttgaa ggtaaacagc cgaataaacg caaatttgac aacgacatgc aacacgaatt
    28981 gcgtatcttg gaagaattgt tttattacgc gcacgatgag caatcccgct atatttcaga
    29041 actgaaaaat gagttgcgaa aagacggtta tttgcctgaa aaagacgatg ataaggtatt
    29101 ggcaacattt aaactcaaat ctgaatttgc cgctaataag gattttagag aattgttaat
    29161 ttgggcgaat aaaaaaatcc ccaacccaaa cgccaaaagc aataatgcag acagcctaaa
    29221 agccaatcca caaacgcttt cattccaagt tcacggtaat caactgttgc aggaaacgca
    29281 atttacagcc gatgaaaatg atgaaacagc ccgacaaatc ggcacacaaa ataattttac
    29341 tcaaaccata aaaatgagtg aaatggagcg gcacattttc aataaagcat tacatatcaa
    29401 agggaaaaat agtcaatctt tattccattt caaccgtttg caaagcaagc tcgacattca
    29461 aaatcgcaat gaattgcaaa ataacttgtt gaaagactgg caaattgaat ttttaggatt
    29521 agggcaagac aaacaggttc gcctggacga taaacttgca ggctgcctga aaatcttgga
    29581 aatggttgaa aaacatctga atgaaagtga tatgccgttt atcggtacaa aagaatttac
    29641 acctaaaaaa ttatgggaaa ttttcggcac gccaaaacaa aaatgggtca aaaaagatga
    29701 tgtaaaaacg actattgcca cgcaaaatga ttggtatgtg atgaataatt ttgccggaac
    29761 gggtttggaa gaagcgttaa ttcaatttat ttcagcgcgt ttgggcgatt tgaaatctaa
    29821 atatgacgtt catttaatcc gtaatgaaga agtgtttaaa ttgaataact ttgccgatgg
    29881 tgaaggattt atgccggact ttattttatt gctgaaagat aaacaaaaat cttcttccaa
    29941 tggtgtgaac gactttttgc attaccaaat tttcattgag cccaaaggtg agcatttagt
    30001 ggaaactgat ggatggaaag aagaattttt agaagccatc actgtggaat atgggatgga
    30061 taagatcctg caaaaagata caccgcatta tcgtttgatc ggtttaccgt tttttactga
    30121 tcatcagaaa aatggacaat ttacggagtc attcccttta ggtgcggcat cgcttgaaaa
    30181 aatgaagtag tgcattgcag gcaaccccgt ttgacaaaac ttcctttaca aaagggcgtt
    30241 ttgtcagata tttaatcaac acattattaa aatacagcca aattttaatg ccgtccgaac
    30301 cctgtgttca gacggcatcg tatttttcag tatctaaacc gtttccctgc cccaatcttt
    30361 gcctctcaaa atcgaagcat cgacatcttg aatatcgcgg tgtcccgtaa acgccataga
    30421 tatatccatt tctttataca ggatttccag cgcacgggtt acgccttctt caccatacgc
    30481 acccaaacca tacagaaacg cccgacctat cattgtacct ttcgcaccca aagcccacgc
    30541 cttcaaaata tcctgaccgc tgcggatgcc gctgtccatc caaacttcga tgtcgctgcc
    30601 gactgcacta acgatgtcgg gcaaggcttt gatggcggac acggtatcgt cgagctggcg
    30661 gccgccgtgg ttggaaacga ccaatgcgtc cgcaccgctt ttcgctgctt tttccgcatc
    30721 ttcaggttcc ataatgcctt tgataatcag cttgccaccc cacaaatctt taatgcgtgc
    30781 aacgtcgtcc cagctcaggc gcgggtcgaa ttgttcggaa gtccatgaag acagcgagga
    30841 caaatcgccg acgtttttgg cgtgtccaac gatattgcgg aacgtgcggc gttccgtgtt
    30901 cagcattttc atacaccatt cgggcttggt cgccagattg attaaattgg cgatggtcgg
    30961 tttcggcggc gcggacaggc cgtttttgat gtctttgtgg cgttgcccca aaacctgcaa
    31021 atcagcggtc aataccaatg ccgaacattt ggcatccttc gcgcgcttaa tcaggttttc
    31081 cataaactcg cggtcgcgca tcacataaag ctgaaaccaa aatggtgcgg aagtgttctc
    31141 ggcaacgtct tcaatcgagc agatagacat cgtggacagc gtaaacggaa tgccgaactt
    31201 ctccgccgcc cgcgccgcca agatttcacc gtcggcgtgt gccataccgg taaaacccgt
    31261 cggcgcaatc gccaccggca ttttcacatc ctgcccgatc attttggttt ccaggcttcg
    31321 accttccata ttgaccaata ccttttgtcg gaagcggata tctttaaaat ccgaagtgtt
    31381 ttcgcggtag gtagtttccg tccacgaacc cgaatcgatg taatcgtaaa acatacgcgg
    31441 cattttgcgc ttggcaacgc ggcgcaagtc ttcgatgcag gtcattttgc tcaaatcacg
    31501 tttcatttgt cgccccctga atacctgaat aactttatat gaaatagata atgtatatca
    31561 atattgatta taaggcaaat catttcaaca tttactgcac ccgccgccgc tcccacttca
    31621 agcggcataa ggtttagaat tcaaaaataa tatattaaaa tcaaaatatt aaaaatcaat
    31681 caatctgccg atttaaacag ccaatcgcgc aatccgcccc catacttgac tgaaacactc
    31741 agatattgga caattccgcc cactataaaa aaagccgaca cgggcaacca ccaccatgag
    31801 actgaccacc aaagggcgtt tcgccgttac cgctatgctg gatttggcga tgaacgcgca
    31861 aaccggcgcc gtcaaactca gtgccatcag cgaacgccaa aacatatccc tctcctatct
    31921 cgagcaattg ttcggcaaac tccgccgcgc cggacttgtt gaaagcctgc gcgggcccgg
    31981 cggcggctac atcctcgccg caccggcggc acgcatcaac atcgcccaaa tcatcgccgc
    32041 cgccgaagac cggctggacg caacccaatg cggcagcaaa gccaactgcc accacggcgc
    32101 gccctgcctg acgcacgatc tttgggagaa tttaaacaaa accatcaacg actacctcgg
    32161 cagcgttacc ctgcaaagca tcatcgaaca gaaaaacaac ggcgacggca gccgcgtcgt
    32221 ccaatttaca cacatccatt aaataacacc cgaaaaagaa agagcaaacc atgaccgtca
    32281 aaacccccgt ttacctcgac tacgccgcca ccacccccgt tgacaaacgc gttgccgaaa
    32341 aaatgattcc ctatctgacc gaaaccttcg gcaacccagc ctccaacagc cacagcttcg
    32401 gctgggaagc agaagaagct gtagaaaaag cacgtgcaga cattgccgcc ctgattaacg
    32461 ccgactctaa agaaatcgtt ttcaccagcg gcgcaaccga gtccaacaac ctcgctatca
    32521 aaggcgcggc gcacttctac aaatctaaag gtaatcacct catcactgta aaaaccgaac
    32581 acaaagccgt actcgacacc atgcgcgaac tcgaacgcca aggttacgaa gtaacttatc
    32641 tggacgtaca agaaaacggt ttggttgatt tagacgtact gaaagccgcc atccgcgaag
    32701 acaccatcct cgtttccgta atgtgggtaa acaacgaaat cggcgtggtt caagatattc
    32761 ctgccatcgg cgaaatctgc cgcgaacgca aaatcatttt ccacgttgac gcagcacaag
    32821 catgcggcaa agtgcctgtt gatgttgaag ccgcaaaagt tgatttgctg tctatgtccg
    32881 gccacaaagt atacggccct aaaggcatcg gcgccctgta tgtacgccgt aaaccacgtg
    32941 tccgcctcga agcccaaatg cacggcggcg gtcacgaacg cggcttccgc agcggcacat
    33001 tgccgaccca ccaaatcgtc ggtatgggcg aagccttccg catcgccaaa gaagaactcg
    33061 aacaagatat ggcgcactac cgcaaactgc gcgacatctt cctcaaaggc atcgaaggca
    33121 tcgaagaagt ctatgtcaac ggcgacctcg aacaccgcgc cccgaacaac ctgaacgtca
    33181 gcttcaactt cgtcgaaggc gaaagcctga ttatggcggt gaaagaactc gccgtatcca
    33241 gcggctccgc ctgtacttct gccagtctcg aacccagcta cgtcctgcgc gcgctcggcc
    33301 gcaacgatga actggcgcac tcatccctgc gcatcacctt cggtcgcatg accaccgaag
    33361 aagaagtgca attcgccgcc gaactgatta aatccaaaat cggcaaactg cgcgaactgt
    33421 cgccgctgtg ggaaatgttc aaagacggga ttgatttgaa ctcgattgaa tgggcagcgc
    33481 attaaagcgt accaacatgc cgtccgaacc tttagacggc attccaaaaa caaagcaatc
    33541 aagagaaaat atgaacgaac aagatttaga tttggacaat ctcgacaacc tgcttgaaga
    33601 ttttgacggc gttaccgtgg aaggcggcgt cgattcggaa aacgacgacg gctgtgaagg
    33661 cggcgcgtgc aaaatctaac tcaggccgtc tgaaaaccgg caaacaaacc acttaaatca
    33721 tcaaaacatt aaggaaacca catcatggca tacagcgata aagtaatcga ccactacgaa
    33781 aatccccgca acgtcggtac tttcgacaaa aacgacgaat ctgtcggcac cggcatggtc
    33841 ggcgcaccgg cctgcggcga cgtgatgcgc ctgcaaatca aagtgaacga tgaaggcatc
    33901 atcgaagatg cgaaattcaa aacttacggc tgcggttccg ccatcgcttc gtccagcctg
    33961 attaccgagt gggtcaaagg caaaagcctg gatgacgcgc tggcaatcaa aaacagcgaa
    34021 atcgccgaag agttggaatt gccgccggta aaaatccact gctccatctt ggctgaagat
    34081 gcggtaaaag cggccgttgc cgactaccgc aaacgtcagg aaaacagata aagcccttca
    34141 gacggcatcg tcccgcaatg ccgtccgaac cacccgccgc ttcaggtccg tccggggcgg
    34201 tacaacaagg aagaaatatg attaccctta ccgagaatgc cgcaaaacac atcaatgact
    34261 atctcgccaa acgcggcaaa ggcttgggcg tacgcttggg tgtgaaaacc agcggctgct
    34321 cggggatggc gtacaacctt gaatttgtcg atgaagccga cggcgacgac ctgattttcg
    34381 aaggacacgg cgcacgcatt tatatcgacc cgaaaagcct ggtttatctg gacggcacac
    34441 aagtcgatta caccaaagaa ggtttgcagg aaggtttcaa atttgaaaac cccaatgtca
    34501 aagactcctg cggctgcggc gaaagcttcc atgtttaaga cataaaaatg gcgggaccgt
    34561 atcaaaaccg tcccgccatt ttttcgcttc ctgcctgttg tagctgcctt tgcctttcct
    34621 tttccgttcc accttgtgcc ggaacaaatc ggatttcact aaggctttta aagcattgtc
    34681 gcgtattttg cctttattgt gctgcacttt gccgcccata ttcagtcctt tcgtttaaga
    34741 agctgcagat tataaggcaa aaacagtttt ctgccaaaat cttacattta tcatcctact
    34801 atgtcccaat atttcaccct cttccggatt gaacccgctt tcgatatcga caccgaaaac
    34861 ttggaacaaa cctaccgcgc cttggccgcc cgtttccatc ccgataaatt cgcttcagct
    34921 tccgcctttg agcaaaagca ggcagtgatg atgtcttcca ccatcaacga tgcctaccgc
    34981 accttgaaaa accccatcga ccgcgccgcc tacctgctga aaaaatcggg catcgatgcc
    35041 gacgcgccgg agcataccgc tttcgccccc gaattcctta tgcagcaaat ggaatggcgc
    35101 gaaacgctga tggaggcacg ggcaggcaac gaccttgaat ccttgaaaaa tctcgacaac
    35161 gaaatccgcg acgaacaaga aaaactgctc tgcggtctga aacagtcgtt tgcccgacaa
    35221 gattacgaca cagccgcaca acaagtccgc caaggcaggt ttctcgacaa actccgcaac
    35281 gaaatttcct cggcattata atccgcaccg tgtttcagac ggcgtaaccg ccgcaccgtt
    35341 ccgcgtcaaa atatgctaaa ataagcaaca attttttgcc atacgaaaca ttgaaaccat
    35401 gaccgacgca accatccgcc acgaccacaa attcgccctc gaaaccctgc cggtaagcct
    35461 tgaagacgaa atgcgcaaaa gctatctcga ctacgccatg agcgtcattg tcgggcgcgc
    35521 gctgccggac gttcgcgacg gtctcaagcc ggtacaccgc cgcgtactgt acgcgatgca
    35581 cgaattgaaa aacaactgga atgccgccta caaaaaatcg gcgcgcattg tcggcgacgt
    35641 catcggtaaa taccaccccc acggcgatac cgccgtatac gacaccatcg tccgtatggc
    35701 gcaaaatttc gctatgcgtt atgtgctgat agacggacag ggcaacttcg gatcggtgga
    35761 cgggcttgcc gccgcagcca tgcgctacac cgaaatccgc atggcgaaaa tttcccacga
    35821 aatgctggca gacattgagg aagaaaccgt caatttcggc ccgaactacg acggtagcga
    35881 acacgagccg cttgtactgc cgacccgttt ccccacactg ctcgtcaacg gctcgtccgg
    35941 catcgccgtc ggcatggcga ccaatatccc gccgcacaac ctttccgata ccgtcaatgc
    36001 ctgcctgcgc ctgctcgatg cacccgacac cgaaatcgac gaactgatcg acattatcca
    36061 agcccccgac ttcccgaccg gggcaaccat ctacggcttg agcggcgtgc gcgaaggcta
    36121 taaaacaggc cgcggccgcg tcgttatgcg cggtaagacc catatcgaac ccataggcag
    36181 aaacggcgaa cgcgaagcca tcgttatcga cgaaatcccc tatcaggtca acaaagccaa
    36241 gctggtcgag aaaatcggcg atttggttcg ggaaaaaaca ctggaaggca tttccgagct
    36301 ccgcgacgaa tccgacaaat ccggtatgcg cgtcgttatc gagctgaaac gcaacgaaaa
    36361 tgccgaagtc gtcttaaacc aactctacaa actgactccg ctgcaagaca gtttcggcat
    36421 caatatggtg gttttggtcg acggacaacc gcgcctgttg aacctgaaac agattctctc
    36481 cgaattcctg cgccaccgcc gcgaagtcgt tacccgacgt acgcttttcc ggctgaagaa
    36541 ggcacgccat gaagggcata tcgccgaagg caaagcggtc gcactgtcca atatcgatga
    36601 aatcatccgg cttatcaaag aatcgcccaa cgcggtcgag gccaaagaca aactgcttgc
    36661 gcacccttgg cgcagcagcc tcgttgaaga aatgctgacg cgttccggtc tggatttgga
    36721 aatgatgcgt ccggaaggat tggctgcaaa catcggcttg aaagagcaag gttattacct
    36781 gagcgagatt caggcagatg ctattttacg catgagcctg cgaaacctga ccggcctcga
    36841 tcaagaagaa attgtcgaaa gctacaaaaa cctgatgggt aaaatcatcg actttgtgga
    36901 catcctctcc aaacccgaac gcattaccca aatcatccgc gacgaactgg aagaaatcaa
    36961 aaccaactat ggcgacgaac gccgcagcga aatcaacccg ttcggcggcg acattgccga
    37021 tgaagacctg attccgcaac gcgaaatggt cgttaccctg acacatggcg gctatatcaa
    37081 aacccagccg accaccgact atcaggcgca gcgtcgcggc gggcgcggca aacaggcggc
    37141 tgccaccaaa gacgaagact ttatcgaaac cctgtttgtt gccaacacgc atgattattt
    37201 gatgtgcttt accaatttgg gcaagtgtca ttggattaag gtttacaaac tgcccgaagg
    37261 cggacgcaac agccgcggcc gtccgattaa caacgtcatc cagttggaag aaggcgaaaa
    37321 agtcagcgca attctggcag tacgcgagtt ccccgaagac caatacgtct tcttcgccac
    37381 cgcgcaggga atggtgaaaa aagtccaact ttccgccttt aaaaacgtcc gcgcccaagg
    37441 cattaaagcc atcgcgctca aagaaggcga ctacctcgtc ggcgctgcgc aaacaggcgg
    37501 tgcggacgac atcatgctgt tctccaactt aggtaaagcc atccgcttca acgaatactg
    37561 ggaaaaatcc ggcaacgacg aagcggaaga tgccgacatc gaaaccgaaa tttcagacgg
    37621 catcgaagat gaaaccgccg acagcgaaaa cgcactgccg agcggcaaac acggtgttcg
    37681 cccgtccggt cgcggcagcg gcggtttgcg cggtatgcgc ctgcctgccg acggcaaaat
    37741 cgtcagcctg attaccttcg cccctgaaac cgaagaaagc ggtttgcaag ttttaaccgc
    37801 caccgccaac ggatacggaa aacgcacccc gattgccgat tacagccgca aaaacaaagg
    37861 cgggcaaggc aatattgcca ttaacaccgg cgagcgaaac ggcgatttgg tcgccgcaac
    37921 cttggtcggc gaaaccgacg atttgatgct gattaccagc ggcggcgtac ttatccgcac
    37981 caaagtcgaa caaatccgcg aaaccggccg cgccgcagca ggcgtgaaac tgattaactt
    38041 ggacgaaggc gaaaccttgg tatcgctgga acgtgttgcc gaagacgaat ccgaactctc
    38101 cgacgcttct gtaatttcca atgtaaccga accggaagtc gagaactgaa aatcatctcc
    38161 cgatgccgtc tgaagattca gacggcattt attttatccc tcatccgtca tccagcttct
    38221 cacaatatag cggattatag tcaattaaaa acaaggggct gtcctagata actagggaaa
    38281 ttcaaattaa gttagagttg cccctatgag aaaaagtcgt ctaagccagt ataaacaaaa
    38341 taaactcatt gaactgtttg tcgcaggtgt aactgcaaga ac
//
LOCUS       DAXHTY010000006        37952 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXHTY010000006 DAXHTY010000000
VERSION     DAXHTY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37952
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.006"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(91..450)
                     /gene="rnpB"
                     /locus_tag="ACFNMK_000274"
     ncRNA           complement(91..450)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFNMK_000274"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(529..1632)
                     /gene="prfB"
                     /locus_tag="ACFNMK_000275"
     CDS             complement(529..1632)
                     /gene="prfB"
                     /locus_tag="ACFNMK_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC6363619.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIATGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPAGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1735..2628)
                     /locus_tag="ACFNMK_000276"
     CDS             complement(1735..2628)
                     /locus_tag="ACFNMK_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC6363620.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2970..3419
                     /locus_tag="ACFNMK_000277"
     CDS             2970..3419
                     /locus_tag="ACFNMK_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6363621.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3493..3801
                     /locus_tag="ACFNMK_000278"
     CDS             3493..3801
                     /locus_tag="ACFNMK_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363622.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3917..4759
                     /locus_tag="ACFNMK_000279"
     CDS             3917..4759
                     /locus_tag="ACFNMK_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC6363623.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDAERVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4749..5495
                     /locus_tag="ACFNMK_000280"
     CDS             4749..5495
                     /locus_tag="ACFNMK_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC6363624.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIQQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5517..6104
                     /locus_tag="ACFNMK_000281"
     CDS             5517..6104
                     /locus_tag="ACFNMK_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC6363625.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6110..6289
                     /locus_tag="ACFNMK_000282"
     CDS             6110..6289
                     /locus_tag="ACFNMK_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC6363626.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            6521..6961
                     /locus_tag="ACFNMK_000283"
     CDS             6521..6961
                     /locus_tag="ACFNMK_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC6363627.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(7218..7565)
                     /locus_tag="ACFNMK_000284"
                     /pseudo
     CDS             complement(7218..7565)
                     /locus_tag="ACFNMK_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7593..8195)
                     /locus_tag="ACFNMK_000285"
     CDS             complement(7593..8195)
                     /locus_tag="ACFNMK_000285"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6363628.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(8229..9500)
                     /gene="purD"
                     /locus_tag="ACFNMK_000286"
     CDS             complement(8229..9500)
                     /gene="purD"
                     /locus_tag="ACFNMK_000286"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC6363629.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPIQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            9658..9975
                     /locus_tag="ACFNMK_000287"
     CDS             9658..9975
                     /locus_tag="ACFNMK_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC6363630.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            complement(10027..10638)
                     /locus_tag="ACFNMK_000288"
     CDS             complement(10027..10638)
                     /locus_tag="ACFNMK_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC6363631.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(10701..11636)
                     /locus_tag="ACFNMK_000289"
     CDS             complement(10701..11636)
                     /locus_tag="ACFNMK_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC6363632.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            complement(11647..12396)
                     /locus_tag="ACFNMK_000290"
     CDS             complement(11647..12396)
                     /locus_tag="ACFNMK_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC6363633.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDAKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            complement(12623..13591)
                     /gene="waaC"
                     /locus_tag="ACFNMK_000291"
     CDS             complement(12623..13591)
                     /gene="waaC"
                     /locus_tag="ACFNMK_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC6363634.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPGVELHWLCEAGFTDI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPIADLVYQALMDCVAADKG"
     gene            complement(13629..14264)
                     /locus_tag="ACFNMK_000292"
     CDS             complement(13629..14264)
                     /locus_tag="ACFNMK_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC6363635.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIVGGLATDYCVKTTVLQL
                     LKGGNWQVIVNEAACRGIAPDTIETAWQEMRSAGAVILKNAEEIKKHINNQ"
     gene            14378..15058
                     /gene="tsaA"
                     /locus_tag="ACFNMK_000293"
     CDS             14378..15058
                     /gene="tsaA"
                     /locus_tag="ACFNMK_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC6363636.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAKACIELNPKFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            15127..15246
                     /locus_tag="ACFNMK_000294"
                     /pseudo
     CDS             15127..15246
                     /locus_tag="ACFNMK_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(15316..16320)
                     /gene="gap"
                     /locus_tag="ACFNMK_000295"
     CDS             complement(15316..16320)
                     /gene="gap"
                     /locus_tag="ACFNMK_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6363637.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            16764..19337
                     /gene="mutS"
                     /locus_tag="ACFNMK_000296"
     CDS             16764..19337
                     /gene="mutS"
                     /locus_tag="ACFNMK_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC6363638.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKSIADIERIAARPRDLAALRDSLFALSEIELSAECSSLLGTLKAVFPENLSTAE
                     QLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERERTGLSTL
                     KVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVLTAQEQAL
                     ALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEFADYPVIH
                     IENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIVLLAHTGC
                     FVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSLVLMDEVG
                     RGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAALNMHLSALEQGQ
                     DIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRPQLDIFST
                     MPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRLKDLCKSV
                     S"
     gene            complement(19460..19951)
                     /locus_tag="ACFNMK_000297"
     CDS             complement(19460..19951)
                     /locus_tag="ACFNMK_000297"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6363639.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(19955..20350)
                     /locus_tag="ACFNMK_000298"
     CDS             complement(19955..20350)
                     /locus_tag="ACFNMK_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363640.1"
                     /translation="MPSDVGIRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            complement(20371..21765)
                     /gene="gltX"
                     /locus_tag="ACFNMK_000299"
     CDS             complement(20371..21765)
                     /gene="gltX"
                     /locus_tag="ACFNMK_000299"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC6363641.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMEWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTRWADL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNEKLAELVKPRLA
                     LRDIHETEKPALEDVLAQVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAESLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            complement(21957..22613)
                     /locus_tag="ACFNMK_000300"
     CDS             complement(21957..22613)
                     /locus_tag="ACFNMK_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC6363642.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIIFTGGRSKKGYM
                     TEAEVGRRYALKQGIPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGWDFFNWLFH
                     "
     gene            complement(22610..22963)
                     /gene="arsC"
                     /locus_tag="ACFNMK_000301"
     CDS             complement(22610..22963)
                     /gene="arsC"
                     /locus_tag="ACFNMK_000301"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC6363643.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            23087..23569
                     /locus_tag="ACFNMK_000302"
     CDS             23087..23569
                     /locus_tag="ACFNMK_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC6363644.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGKVNEKSLTDAVKLAHSKCR
                     "
     gene            23764..24414
                     /gene="ftsE"
                     /locus_tag="ACFNMK_000303"
     CDS             23764..24414
                     /gene="ftsE"
                     /locus_tag="ACFNMK_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC6363645.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            24411..25328
                     /gene="ftsX"
                     /locus_tag="ACFNMK_000304"
     CDS             24411..25328
                     /gene="ftsX"
                     /locus_tag="ACFNMK_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC6363646.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITIYMETAAAQSDSDTVRSLLTRDKRLDNIRFISKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPDQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            25419..25667
                     /locus_tag="ACFNMK_000305"
     CDS             25419..25667
                     /locus_tag="ACFNMK_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6363647.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNAKAQ"
     gene            complement(25734..26912)
                     /locus_tag="ACFNMK_000306"
     CDS             complement(25734..26912)
                     /locus_tag="ACFNMK_000306"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC6363648.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMEKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(26993..28246)
                     /gene="murA"
                     /locus_tag="ACFNMK_000307"
     CDS             complement(26993..28246)
                     /gene="murA"
                     /locus_tag="ACFNMK_000307"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC6363649.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            complement(28466..28541)
                     /locus_tag="ACFNMK_000308"
     tRNA            complement(28466..28541)
                     /locus_tag="ACFNMK_000308"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28506..28508),aa:Lys,seq:ttt)
     gene            complement(28610..29584)
                     /locus_tag="ACFNMK_000309"
     CDS             complement(28610..29584)
                     /locus_tag="ACFNMK_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC6363650.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            complement(29617..30066)
                     /locus_tag="ACFNMK_000310"
     CDS             complement(29617..30066)
                     /locus_tag="ACFNMK_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC6363651.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            complement(30107..31378)
                     /gene="waaA"
                     /locus_tag="ACFNMK_000311"
     CDS             complement(30107..31378)
                     /gene="waaA"
                     /locus_tag="ACFNMK_000311"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC6363652.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRGERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGVLMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRGDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            complement(31441..32889)
                     /gene="gnd"
                     /locus_tag="ACFNMK_000312"
     CDS             complement(31441..32889)
                     /gene="gnd"
                     /locus_tag="ACFNMK_000312"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC6363653.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIIIDGGN
                     ANYSDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            complement(33953..34876)
                     /gene="lpxC"
                     /locus_tag="ACFNMK_000313"
     CDS             complement(33953..34876)
                     /gene="lpxC"
                     /locus_tag="ACFNMK_000313"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC6363654.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
     gene            complement(35834..36340)
                     /locus_tag="ACFNMK_000314"
     CDS             complement(35834..36340)
                     /locus_tag="ACFNMK_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6363655.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNHGKWPDGNSNAGVASSSTIKGKYVEKVEVANGVITATMLSTG
                     VNKEIQGKKLSLWAKRQDGSVKWFCGQPVTRDDANAKDDVAADNDATKKIDTKHLPST
                     CRDAASAS"
     gene            complement(36850..37254)
                     /locus_tag="ACFNMK_000315"
                     /pseudo
     CDS             complement(36850..37254)
                     /locus_tag="ACFNMK_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(37462..37896)
                     /locus_tag="ACFNMK_000316"
                     /pseudo
     CDS             complement(37462..37896)
                     /locus_tag="ACFNMK_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT         9421 a        10342 c         8820 g         9369 t
ORIGIN      
        1 tttttgttaa tccactataa aaacacaacc taaataaaaa tgccgtctga accatatttc
       61 aggtttcaga cggcatttgc gtgtcggatg cacaccggac aggcggtaag ccgggttctg
      121 tctcggacag tcattcctct aggcataccg ttaccagtat gctcaagcaa cctacccgaa
      181 cgctcggcgg gcagcgtcat tgcgttctgt ttggtcttgc tccgaatggg gtttggcctg
      241 ccgcatattg ttaccaaatg cgcggtgcgc ccttaccgca ccttttcacc cttgcctgtg
      301 ctgccaaagc agccatcggc ggttttgctt tctgttccac tttccgtcgc gttaccgcgc
      361 ccggccgtta accggcattc taccctgcgg agcccggact ttcctccccg tatgccttac
      421 gcgatacgcg gcgactgtct gctcgtcccg tgtgcggcgc ggattataac acgaaacgca
      481 aaaatgccgt ctgaaacggt acaggtttca gacggcatac ggttcaaact acacgccctg
      541 tttcaggctg gcttcgataa agccgtccaa atcgccatcc aatacggctt tggtgttgcc
      601 gacttcgtag cctgtacgca agtctttgat acgtgaggaa tccaaaacat acgaacggat
      661 ttggctgccc caacctacat cggatttacc ttcttccaac gcctgtttct cttcattgcg
      721 tttgcgcatt tccaattcat acagtttgga tttcaacatt tccatcgcgg ccgctttgtt
      781 ggcgtgttgc gaacggtcgt tttgacattg caccacaatc cccgccggct cgtgggtaat
      841 gcgcacggcg gagtcggttt tattgatgtg ctgaccgccc gcgcccgatg cgcgataggt
      901 gtcgatacgc aaatcggcgg ggttgatttc gatttcaatg gaatcatcga tttcagggta
      961 aacgaacacg gaggcaaacg aagtatggcg tttgttgttc gagtcaaacg gtgaatagcg
     1021 caccaagcgg tgaacgccgg tttcggtacg cagcaaacca taggcgtatt cgccttccac
     1081 tcggatagtc gcgcggttga tgcccgcgat ttcgccgtcg tcttcttcga ggatttcgat
     1141 tttgaagcct ttgcgctcgg cgtagcggct gtacatacgg aacagcatac ccgcccagtc
     1201 ttccgcttcc gtaccgcccg cgcctgcggt gatgtcgata aagcagttgt tcgggtcggc
     1261 gggctggttg aacatccgtt tgaactccaa atccgccatc tgtttttcca gccccgccac
     1321 atcttcctgc acggcggcaa aaccttcttc gtcgttttct tcgacggtca tttcaatcag
     1381 catgcggttg tcttcgatgc ccgtcgcgat gttgtcgagc gtcaacacga tgccttcgag
     1441 gattttgcgc tctttgccga tttcttgggc gcgtttcggg tcgttccaaa gttcggggtc
     1501 ttcggaaaga ccgataactt cttccaatcg gtctttctta ccctgataat ccatataaac
     1561 tcggatgtct tcgctgcgct tttccaaatc attcagggta ttgttgagct ggttgattac
     1621 ttcggcttcc atgattcttt tgttctttca aaattttagg ggcgtattgt acgggattcg
     1681 ggtatttttt tctatggata aagccttctg gaaacacgtt cggacggcat agcgtcaata
     1741 acggtatgcc gccagtttgc gtttgatttc aggcaatgcg gcacgcgctg cctcctcgcc
     1801 caaccggatg gcgcgttttt tctgatcgaa accgccgact gcacccaaat ccaaaacctg
     1861 tggtttgata actacatccg cctgccccaa ctcattttgc aacgcggaaa cgctcattac
     1921 gttcagcgtc tgatcgagat aagagaagaa gccttggctg atgtttttgc ccggacgggc
     1981 ggaaatatcg acggcaatca cgaaattcgc cccctgccgc cgggcggcac tgacgggcac
     2041 gggctgcgac agaccgccgt caacatatgt atgcctgccg atgataacgg gttggaacac
     2101 attgggaatg gcggcggaag cgcgcacagc ctgcccggca ttcccctgat tgaaagcgac
     2161 ggccttgccg gtttcaaaat cagtagcaac agcggcaaat ttgatgggaa actgctgaat
     2221 ctgcctgccg ccgacttttc ggttgatgta attttgcagc ttttcgcctt tgataaaacc
     2281 actggtggac aaggttaaat cgactaaatc ggttttgcct aaaatctcgg cttccaattc
     2341 gaggcggtcg ggcgacatac ccgatgcaaa aaggctgccg acaatcgaac ctgccgatgt
     2401 accggtaacc accttcacag gaataccgtt ttctttcaaa accttaataa tgcctacatg
     2461 ggcaaatcct ttagatgcgc cgccaccgag tgccaaaccg accactgcgg cgggtttggc
     2521 ggtttgcacc ggcttgcggg cggcattatt tcccgccgtg ccgcaggcgg caagcaacgc
     2581 ggcggcggcg attgccaaaa gcggtctgat ttttgaaaac gttaccatat tttccattcc
     2641 tttatatatc gcaccccgtc aaaaagaggg attgcttttc ttaacaaccc cctttgacag
     2701 ccaagcaaat gggggctttg ttaagtcatc atcaaaatta atatttcttt ttttccttta
     2761 cggaaattat atttgaaggc atactatcca aggtgggaat tatctcacaa caccgccgtt
     2821 atccaaatat cccgcctttt tcccttcctt tccatcaaaa tactttcttt ttatattcat
     2881 taacttgtta aatcattggc tgccgggtgt cagtttttcc gacaaaatcc gtctaatggg
     2941 gtatcaacag aaccaaaaca ggaacactta tgaaaatcgg aacaacttgg cagacggcat
     3001 ccgctatgct ggttttgcgt ctgtttgccg catatgaatt tttggaatcg ggtttgcaaa
     3061 aatggaacgg ggagaattgg ttttctgaaa tcaacgatca attcccattc ccgttcaact
     3121 tgctgccgga cgcgttaaac tggaatctcg ctatgtatgc ggagctttta ctgcccgtac
     3181 tgttgctttt gggtttggca acgcgtctgt cggcattagg gctgatggtc gttaccgccg
     3241 tcgcttgggc tgcggttcac gccggttcgg gttacaatgt ctgcgacaac ggttataaaa
     3301 tggctttaat ttatatcgtg gtattaatcc cgctgctttt ccagggtgcg ggcggatggt
     3361 cgctggatac gctgctgaaa aaacggtttt gcccccaatg ccgtctgaag caagattgat
     3421 tcagtcgtgg aatctgactt taaacattcc aaccttatct cgttaacttg atattctgaa
     3481 aaggaaatag aaatgaacaa aaacattgct gccgcactcg ccggtgcttt atccctgtct
     3541 ttggccgccg gtgcagtggc tgccaacaaa ccggcaagca acgcaacagg cgttcataaa
     3601 tccgcccatg gctcttgcgg cgcgtccaaa tctgccgaag gttcgtgcgg cgcggctggt
     3661 tctaaagcag gcgaaggcaa atgcggcgag ggcaaatgcg gtgcgaccgt aaaaaaaacc
     3721 cacaaacaca ccaaagcatc taaagccaag gccaaatctg ccgaaggcaa atgcggcgaa
     3781 ggcaaatgcg gttctaaata atcccacccc ttcaaaccaa gccgcgtttt tcagtaaaat
     3841 gcggcttttt taacggcaaa caaagatttt ttaacaagca catcattctt ttgtgccatc
     3901 cgaaccgggt aaaaatatga ttcaacacgc aggcttgggc taccgccgcg acttggcgga
     3961 agactttctc tcgctttccg aaaacagccc gatatgcttt atcgaagccg caccggaaaa
     4021 ctggctgaaa atgggcggct gggcgcgcaa acagtttgac cgtgtagcgg aacggctgcc
     4081 gctggcgttg cacggattgt ctatgtcgct gggcgggcaa gcaccgctgg atactgattt
     4141 gatagacggc atcaaagaaa tgatgcgccg ttacgattgc acgtttttct ccgaccattt
     4201 gagctactgc cacgacggcg gtcatcttta cgatttgttg ccgctgccct tcaccgagga
     4261 aatggtgcat catacggcgc ggcgtatccg cgaagtgcaa gaccgtttgg gctgccgcat
     4321 cgccgtggaa aacacgtcct actatctgca ttccccgctt gccgagatga acgaggtcga
     4381 gttcctcaac gccgtcgcac gtgaggccga ttgcggcatt catctggatg tgaacaatat
     4441 ctacgtcaac gccgtcaatc acggtctgct gtcgccggag gcttttttga aaaacgttga
     4501 cgcagagcgc gtgtgctata tccatattgc cggacatgac gtggaaacgc cggaattgtt
     4561 gattgataca catggcgcgg cagttttgcc gactgtttgg gacttgctcg aacttgccta
     4621 tgccaagctg ccgacgattc cgcccaccct gttggaacgc gattttaatt tcccgccttt
     4681 tgccgaactc gaagccgaag tcgccaaaat cgccgattat caaacgcgtg ccggaaagga
     4741 ataccgccgt gcagcctgaa acctccgccc aataccagca ccgtttcgcc caagccatac
     4801 gcgggggcga agccgcagac ggtctgccgc aagaccgact gaacgtctat atccgcctga
     4861 tacgcaacaa tatccacagc tttatcgacc gctgctatac cgaaacgcgg caatactttg
     4921 acagcgaaga atggagccgt ctgaaagaag gtttcgtccg cgacgcgcac gcccaaacgc
     4981 cctattttca agaaatcccc ggcgagttcc tacaatattg ccaaagcctg ccgcttttag
     5041 acggcatttt ggcactgatg gattttgaat atacccaatt gctggcagaa gttgctcaaa
     5101 ttccggatat tcccgacatt cattattcaa atgacagcaa atacacacct tcccctgcgg
     5161 cctttatcca gcaatatcga tatgatgtta ccgatgattt gcatgaagcg gaaacagcct
     5221 tgttaatatg gcgaaacgcc gaagatgatg tgatgtacca aacattggac ggcttcgata
     5281 tgatgctgct agaaataatg ggttcctccg cgctttcgtt tgacaccctc gcccaaaccc
     5341 ttgtcgaatt tatgcctgag gacgataatt ggaaaaatat tttgcttggg aaatggtcag
     5401 gctggactga acaaaggatt atcatcccct ccttgtccgc catatccgaa aatatggaag
     5461 gcaattcccc gggccaaaac catctatccg cataaaatta ccttgttccc gatactatgc
     5521 cgctacccga cctgaccgat gccgaattaa tagagtcgcg taaactgctt ctgcattttg
     5581 cgcggcttca gttgcccgac caccctgatt tggctgaaga tttagtgcag gaaacattgc
     5641 tgtccgcata cagcgcaggc gacagttttc aaggcagggc acttgtcaac agctggcttt
     5701 ttgccatatt gaaaaacaaa attattgacg cattacgtca aatcggaagg cagaggaaag
     5761 tctttaccac actggatgac gagctactgg atgaagcatt tgaaagccat ttttcccaaa
     5821 acgggcattg gacgccggaa gggcagccgc aacattggaa cactccggaa aaatcattaa
     5881 acaacaacga attccaaaaa attctgcaaa gctgcctata caacttgcct gaaaacaccg
     5941 cacgggtatt taccctgaag gaaatactcg gtttttcatc cgacgaaata caacaaatgt
     6001 gcggtatcag cacgtccaac taccacacca ttatgcaccg cgcccgagaa tcattgcgcc
     6061 aatgcctgca aatcaaatgg ttcaaccaag aaaacccgaa gtaaacgtta tgaaaaaatg
     6121 ccgcgatatc gccctgcttc tttccaaaca tcaggaccgg gaaaccaccc cgggcgagaa
     6181 gatttccata tacacacacc tgctgttctg cccacattgc cgagaatata aaagacagct
     6241 tcaaaccatc aaaatatcac tggcaaaaac aaccagaact tcaaaataaa tgccgtctga
     6301 aaaggcttca gacggcataa gctgacggaa aaaaatcaaa ccgatttact gttatcggca
     6361 gttcatccat aatacacgct tcaaaagcag catattcctc catacggaat atataaatac
     6421 gcaaaacacg aaggctgcat caatttgctt tatttgcctt atttcacaga cggcgctacc
     6481 cctcccgccc aacccgttct ttctgaatga gcagatttca atgattaagg aaaccctaat
     6541 gcgcccaatc ttcctatctt tcgttttact ccctattttg ataaccgcct gcagcacacc
     6601 ggacaagtct gcccgatggg aaaatatcgg cacaatctca aacggcaata ttcatacata
     6661 tatcaataaa gacagcgtga gaaaaaacgg aaatctgatg attttccaag ataaaaaagt
     6721 tgttaccaat ctaaaacaag aacgttttgc caacaccccc gcatacaaga ctgccattgc
     6781 cgagtgggaa atccactgca acaacaaaac ataccgctta agttcgctac agttgtttga
     6841 tacaaaaaac acggaaattt ccacacaaaa ctacacagcc tcttccctcc gcccgatgag
     6901 catcctgtcc gggacattaa ccgaaaaaca atatgaaacc gtatgcggaa aaaaactctg
     6961 attgcaactt atacacaaac ctacccacaa accttgtcat aaaaatgccg tctgaaatac
     7021 tgaaatatca gcatttcaga cggcattttg ccattccctg aaaattatcc acaaagttat
     7081 ccacattatt tttaaaaccg gcttccatcc gaaatatagt ggattaacaa aaatcaggac
     7141 aaggcgacga agccgcagac agtacaaata gtacggcaag gcgaggcaac gccgtactgg
     7201 tttaaattta atccactata caaatacaga aactcaagaa aataaccttg tgtattgacc
     7261 atctcaagca attcagaaaa atcaagaaat tttctgaccg taaacaaacg tttccctaaa
     7321 aaaacgatgt cttcaaaaat atcgaacaaa taatcatctt cggcgttttc atttttatgg
     7381 gttaaaacaa cacgggaaaa atctgttttc agatgcttgc ccgcttgatt gttcggatta
     7441 ttgtccggaa cgacaaaacc gtcctcaaaa ttaaagcaga cgttgcgtcc ttctaccttt
     7501 atctctgtgc aataacaatc atgtagagaa atgctatccg aaaaattttt tctttgtgta
     7561 tgcaaaaaaa gttttcattc aagtacccat atctaacgca aacgtttacc tgtttccccg
     7621 tcaataatct gactcggcga tttctgcctg ccgattctcc caccaacaat ccacacatcg
     7681 cgtccgaatt gccttctgac ttccctctcc gtccgacacg cgcgtttgcc tgcgcggttg
     7741 cacgaagtcg agaccaaagg cgtttgcaaa gcctgacaca agcggcgcgc acctacatgg
     7801 gcgggaaccc tgaccgccaa cttgctgcgc tgtttcccgc gtaactcggg taagacacag
     7861 gatttggcgg ataataggaa cgttttaggg gcgggccatt cttttctaag catatcctga
     7921 agattttcag acggcatttg aagtaaaggc tgcaattgct caaattgatt cccgatgaca
     7981 atcataccct tgtgttgcgg tctttttttc aaatgcgcca acttaccgag tgctttggct
     8041 aatgtcggaa gacaccccaa gccataacaa gattcggtcg gataagcgac caaaccgcct
     8101 tttttcaaat aaacgcttaa cttacgttgc gctgatgctg cgataattct cggaaataac
     8161 ataatataaa ataccgtctg aagcacatca gtcatacttg gcttcagacg gcatcatcct
     8221 ctttctaatt aacggttaat cgctttatcg gcaatgtctt tacggtattg catcccgtcg
     8281 aaactgattt tttccaacgc gccatatgcc ttagctttcg cttgcgccac attatcgccc
     8341 aatcccacaa cacacaatac gcgtccgccg ttggtcaata cgtcaccttt ctcgtttgcc
     8401 gttgtacctg catggaaaac tttgcctact tggttggcag catccagacc ggaaataaca
     8461 tcgccttttt tgggcgtttc ggggtaattt tgcgccgcca gtaccacgcc cacggcagtt
     8521 tgcgggctcc attccgcggt tacgctatcg agtttgccgt ctattgccgc ttcaaccaaa
     8581 tccgataagt cgctgttcag tcggctcata atcggctggg tttcaggatc gccgaaacgg
     8641 cagttaaact caatcgtatg gggtgcaccg ctttgatcaa tcatcaaacc tgcgtacagg
     8701 aaaccggtga actcatgccc ctccgctttc atccctgcta cggtcggcaa aataatttca
     8761 ttcatcgcgc gttcgtacac aacaggcgtt accacaggcg cagggctgta cgcacccata
     8821 ccgcccgtat tcagaccttt gtcgccgtct aaaagacgct tgtggtcttg gctggttgcc
     8881 ataggcagta cattattgcc atcaaccatg acgataaaac tcgcttcttc gccttgcagg
     8941 aaatcttcaa tcacaatacg cgcgccggca ttgcccattt tgttgtccag cagcatatca
     9001 tcaatcgcag catgcgcttc atccaaagtc atcgccacaa tcacgccttt acctgccgcc
     9061 aaaccatcgg ctttgataac gataggcgca cctttctgat tgacgtaatc atgtgcggca
     9121 tcggcgtttt caaaggtttg atattgcgcg gtcggaatat tgtatttcgc cataaatgct
     9181 ttggcgaaat ctttggaact ttccaactgc gccgcatatt gtatcggacc gaatattttt
     9241 agtcctgcag cacggaaatc atccacaaca cctgccgcca aaggcgcttc agggccgacg
     9301 acggtaaaaa caatattttc tttacgacag aattcaatca aatcctgatg cgcagtcaag
     9361 tcgatgtttt gcagcttggg ttcaatcgct gtaccggcat taccagacgc aacaaatact
     9421 gtttccactt taggcgactg cgccaatttc caagccagcg cgtgttcgcg accgccatta
     9481 ccgataacca gcagtttcat gccatctcct tgacaaatat gtacttttaa cgaaaactcg
     9541 atacaaaggg acttttatcc catctgaaga aattttagta gaatcaaaca aaagaccgct
     9601 tcattccact ctgcaaccta ttcaacttat tcataaatta aaaaaggaca agcaaccatg
     9661 caaaaacgta ttgatgaaat ccaaagcaaa taccgcgaat ggtgtcattt actaccgcaa
     9721 ttggaagaag acatccgccg ttggaaacat gccgtcgctt tgattcgcga tatggacaat
     9781 ttttataccc acgaatatca ggcttgtcat cgggctattg aagatggagc ggaggtggat
     9841 ttgcgcacag aaggcgaata cagcattatg agtgaagatg cactatggaa cgcgctgggc
     9901 gaattccatc aactggcttg gttatatttg cgctccagcg tcgatgcctt agacaaatat
     9961 acacaagaag attagtcagc gaagaggtcg tctgaaatac catcacaaag catttcagac
    10021 gacctttcat tcaaaaggct tttccgtatt tacttcaatc tgccgtgtac tcttccaagc
    10081 cgcaacacag gcctcataat ttaccaacga caaactgacc gtcaatcggc aatccaactg
    10141 caaatcccgc tccaatacat ccgcctgata ttgtttagca atgcgtatcg cttcattcaa
    10201 aaacggatat tcacatttca gccaaacagt tttttcaata ttcttttcaa ctacttctgc
    10261 aactgccaac gcttgagccg tcgcctcttt gtacgcatgt atcagacctg gaacacctaa
    10321 caaagtacca ccgaaatagc ggacgaccac gaccaaaacg tcggtaatac ccaccgaatc
    10381 aatctgtccc aaaattggtc gtccagcact tcctgatggc tctccatcat cgttggcacg
    10441 aaattgcaca ccatccacac ccaaacgata ggcatagcac cagtgtcgtg ctttatgatg
    10501 ctcttccttt aacggatcga ggtatttttt cacatcagcc aatgtccgaa tcggataggc
    10561 aaatgcaata aaacggctgc ctttatcttt aaactcagcc tgcgtcgagg aagtaatggt
    10621 tttataagtc gtaatcatgc tgaaatgttt tcagacgacc tcattaataa caaggtcgtc
    10681 tgaacgtttc acgtgaaaca ttaatttttc aatacttctg ttaattgtgg aacgatttca
    10741 aataaatcgc caaccaatcc gtaatcggct acattgaaaa tcggcgcatc agcatcttta
    10801 ttgattgcaa caatcacctt actgtcttgc ataccggcaa cgtgttgaat cgcacctgaa
    10861 ataccgattg caaaatagag ttgcggcgca actactttac cggtttgtcc gacttgagca
    10921 tcgtttggcg catattcggc atcaactgct gcacgggatg caccgattgc cgcacctaaa
    10981 acatccgcca acggtgtcag cacttcattg aatttttccg cactacccaa cgcacgacca
    11041 ccggaaacaa tcacttttgc ctgagtcagt tcaggacgat cggaatggga aagctgacgg
    11101 ttaacaaaac gactcaggtt ttgggcaggg gttgcttcaa cattaattac ctcagcatta
    11161 ccaccttgcg ccgccactgc gtcaaaaacc gtcgcacgga aggtcagcac caatttttct
    11221 gaatcagctt gcacggtttc aaatgcatta cccgcataaa tggggcgcac aaaagtcgtg
    11281 ttatccacaa tttcggtcaa atcagaaatt tgcggtacgt ctaataaggc tgctacgcga
    11341 ggcaaaaggt ttttaccgaa tgtagttgcc gttgctgcaa catagcagta atccgctgcc
    11401 aatttaacaa ccagcggagc caactcttca gccaaacctt cggcataatg ggcagcatct
    11461 gcaaccaaaa ctttttccac acccgccact tgttttgcga actctactac agcagatgca
    11521 ccgcttccgg caaccaataa atcgatttta cccagcttag cggcagcggt aacagcatgc
    11581 aaagtggtag gattcaactg tttgttgtcg tgttcgacaa taatcaatac actcatttca
    11641 gcctcctcaa atcactttgg cttcgttttt caatttttca accaattcgg caacgcttgc
    11701 tacttttacg cctgcctgac gcgccttagg ttcggcaaat ttcaccgttt tcaaacgagg
    11761 tgaaatgtcg gcaaccaaat cgtcaggagt cagtttttcc aaaggttttt tctttgccgc
    11821 cataatattg gggagtttga caaagcgcgg ctcgttcaaa cgcaaatccg cgctgataac
    11881 agcaggcagt ttcaatgcga tggtttcttc gccgccatcg atttcccgca caatctgcac
    11941 ttcgtcgcct tcaatttgta ctttggacgc aaacgtacct tgcgccgcat tcagcaaagc
    12001 agccagcatt tgcgccactt ggttggcatc gtcatcaatc gcctgtttgc ccaaaaagaa
    12061 aatctgcgga ttttctttgt ccgcaacggc tttcagcaac ttggcaactg ccagcggctc
    12121 caatttcgca tcggtttcaa catgaattgc acggtcggca cccatcgcca aagctgtacg
    12181 caacgtttct tcgcatttct tttcgcccaa agaaaccgct acgatctcgc ttacttttcc
    12241 agcttctttc aaacggacgg cttcttccac agcgatttcg tcaaacggat tcatcgacat
    12301 tttgacattg ccgatatcca catccgaacc atcggctttt acacgaactt tgacgttgta
    12361 gtccactacg cgctttactg cgaccagtgc tttcattgaa ccctcctaaa aagaacgctg
    12421 ctttcaccat ccagcgaaac caaaccttct tccctataaa accaaatccg ttttccttaa
    12481 aaacgaattc attcaaaaat ctttcggata atgcttgccg attataccat ttttaaagca
    12541 tttactcaga ctagcggata cacattcctg tatctaataa attggaaaat atcatgccgc
    12601 catatcagtt ttagacgacc ctttagcctt tatctgctgc aacacaatcc atcagcgctt
    12661 gataaaccaa atctgcgatc ggaatctgcc cgatattgcc caaatttttt gcaattggcg
    12721 aaacctgaac gcctgtttta atcggatcgg tatcggtata aatgccgacc acaggttttt
    12781 ccaaggcatt tgccaaatgc agcaaaccgg tatccacgcc gacaattccg accgcgtatt
    12841 tcagcagata cgctgcctgc aataaattta ttttgtcgca cacaatagca aacggcagtc
    12901 cgtctgcaat ttgtttggca cgcgtttttt catcttcatt tccccaaggc aggtaaatat
    12961 tgcattgctg ttcttcattc aacttttgca gcaacgatcg ccagttttcc acaggccata
    13021 acttactgtc ccggctggtc gcatgcaaag ccgcataata cggctgcgct aaatttttca
    13081 gacggcctgc ttcaggaaca gtcaagccaa atacctgcgt ttccggcatc gcatacccaa
    13141 atacttgggc aaacagttca cgattgcgcc aaacagcatt tttccctttc ggtacagcgt
    13201 atgttttcgc atacgccagc gcagccagtc cctcgcgcgc gctattttta tccaaaccat
    13261 aaatagggga tttcgccatt ttagcgaaac acgcgctttt aatcagacct tgactgtcca
    13321 atacaaaatt aaatgcttcc tgccgcaaag tctgtttcag atgacccatt tcccgccaag
    13381 tttcagcctg aaagagatgt ttgcgccatt gccgccattt catcacatgg atttttttca
    13441 caaacggatg caggcgcgca atatctgtaa atccagcttc acacagccaa tggagttcta
    13501 caccagggca ttgtcgcgcc aaatcctcaa ttgcgggcaa agtgtgaatt aaatcgccca
    13561 tactagacaa acggacaagc aaaattttca tattttaaga agtatgtttc acgtgaaaca
    13621 attttaactt attgattatt aatgtgtttt ttaatttctt cggcgttttt taagatgaca
    13681 gcaccggcag aacgcatttc ctgccatgcc gtttcgatgg tatccggcgc aataccccga
    13741 caagccgctt cattgacgat aacctgccaa ttaccacctt taagcaactg caaaaccgtt
    13801 gttttcacac aataatccgt agccaagccg ccgacaatga ccgtatccgt attttgacaa
    13861 cgcagccatt caatcagccc tgtgctcagt ttttcctcaa tatcgtgaaa acacgcgccg
    13921 taaggatgca attcaggatc aacacctttc caaacgcaat aatcgtattc tttggcagaa
    13981 ggcagtccgt ccaataattc atagccgcgc gtaccgacca tcgcatgagc cacccaagtc
    14041 aaatccgcat caggcaaacc tgtcggcttc aacatatcaa cagggttatc cacaagccat
    14101 ttcgctacca tatgatgcgc atctttcgtc atcacgcgca aatccgccaa agcggcttga
    14161 gcattcaact cctcgacaat caaatgcccc tcgttcacgg gcagttcgtc aggacacagt
    14221 ggcgtaaacg ttttttgtgc atcaacatca atggaaacaa tcatctcatt atttcaacgc
    14281 gattaaaatg ccctgtatta taacaaatta ctgcccaaaa gcggtaaaac cgattgtgat
    14341 aagataaggt ttttccaaaa aacttaccca caaccttatg acttatacca ttacccccat
    14401 cggcaccgcc cgctcgccct acaaacagaa attcggcatc gcccgccagc ccggtttggt
    14461 ctccgccgca aaagcctgca tcgagctgaa tcccaaattc accgcagaca gcgtgcgcgg
    14521 gctggaagat ttcgattatg tgtggataag ttttattttt cacggcgtat tggatgaagg
    14581 ctgggcgcaa atggtgcgcc cgccacggct cggcggcaaa caaaaaatgg gcgtgttcgc
    14641 cacgcgcagc ccccaccgcc ccaaccatct cggactctcg ctcctgaaac tcgaacgcat
    14701 cgaaaccggc aaacccgtcc gcctctattg cagcggcgca gacctgctgg acggcacacc
    14761 gattgtggac atcaaacctt atatcccctt tgtcgaatcc aaacccgatg ccgcatccgg
    14821 tttcgtcagc ggcaaacccg tagagttgga agtcgtttgg caggaaaaca tcggcgcgga
    14881 aaatttatct gcaaacacca aaaaccttat cagccaaagc attgcccaag atccgcgccc
    14941 cgcctatcag aatattcccg aacggattta tgtgatgaat attgcagatt acgaagtcag
    15001 atttcaaatc gaggaaaacc gtgcaaccgt tattgatctt tccccaaccc cgctttaaat
    15061 cgggcaaaaa tccggttttg ccgcatagca gttgaacaaa cggctgttgt ttgttcacca
    15121 taagccgcaa tatcaagtta tagcggatta aatttaaatc aggacaaggc aacgaagccg
    15181 cagacagtac aaatagtacg gcaaggcgag gtaacgccgt actggtttaa atttaatcca
    15241 ctatacagat aaacaatgcc gtctgaacgc aatgtgttca gacggcattt acttatccac
    15301 aggtttgttc aagccttaga ttttgcctgc gaagtattcc aaagtgcgga cgagttggca
    15361 ggtgtaggac atttcgttgt cgtaccaggc aacggttttc accagttgtt taccgcccac
    15421 ggtcatcacg cgggtttggg tcgcatcgaa gagcgaaccg tattcgatgc cgacaacgtc
    15481 ggaagaaaca atttgatctt cgttgtagcc gtaagattcg ctggcggcgg ctttcatcgc
    15541 ggcgttgatt tcttctttgg ttacagggcg ttcgaggacg gaaaccaatt cggtcagcga
    15601 gccggtggca acagggacgc gttgggcgga gccgtcgagt ttgccgttca attcggggat
    15661 aaccagaccg atggccttgg cggcaccggt gctgttgggc acgatgttga gcgcggcggc
    15721 tcgggcgcgg cgcaaatcgc ctttgcggtg cggcgcgtca agggtgtttt ggtcgccggt
    15781 gtaggcgtgg atggtggtca tcagaccttc gactacgccg aactcttttt gcaggactgc
    15841 cgccatcggg gcaaggcagt tggtggtgca ggaagcggcg gagataacgg tttcgctgcc
    15901 gtccaaaatg tcttggttta cgccatatac gacggttttc acatcattgc cgccgggtgc
    15961 ggaaatcacg actttgcgcg cgccggccct gatgtgtgct tcggctttgg ttttattggt
    16021 aaagaagccg gtacattcga ggacgacatc cacacccaac tcgccccaag gcaattcttc
    16081 gggattcgga ttggcgaaaa ctttgatttc tttgccgttt accacgatgg catcgtcttt
    16141 taattcggca gtaccttgga aacggccttg cgtgctgtcg tatttgaaaa ggtgcagcag
    16201 catttcggca ggggtcaggt cgttgacagc gacgacttcg atgccgtggg ctttttcaat
    16261 ttgacgcaat gcgaggcggc cgatgcggcc gaaaccgtta atcgctactt taatgctcat
    16321 gtatatactc caagctgtga aacgaaattt caatatctgt attgtattct gaaataaagt
    16381 tacattccac tattacatct aactacttga tgcttatttg atatagatga attttactgt
    16441 ttgcacagat ttccaaaact tttaccatca atatttgaat ttaaaatttt aatgatgatt
    16501 ttgatgattg cggatctgct tgtgtataag ttgcaaatat ccaatatttt cattaccttt
    16561 tcgtcaaata agtttgagtt taaggcttgc cgtataggac agataaacgt ggatgttttt
    16621 tgacttaata atatctccgt ggataacttt gctgttttcc tacttgtctc cacaacctta
    16681 ttgacaggct tacggtcagt ctcattccgt cgaagacaaa accttttgct acaataccgt
    16741 tttcctaatg ataaggcagc cccatgtcca aatccgccgt ttccccaatg atgcagcaat
    16801 acctcggcat caaagcgcaa cataccgaca aactggtgtt ttaccgcatg ggtgattttt
    16861 acgagatgtt tttcgacgat gcggtagaag cggcaaaact tttggatatt accctgacca
    16921 cgcgcggaca ggtggatggc gagccggtca aaatggcagg cgtgccgttt cacgccgccg
    16981 aacaatatct ggcgcgcctg gtcaagttgg gcaaaagcgt ggcgatttgc gaacaggtcg
    17041 gcgaagtcgg cgcgggcaaa ggaccggtgg agcgcaaagt cgtgcgcatc gtaacgcccg
    17101 gcacgctgac cgattccgca ttgctggaag acaaggaaac caaccgcatc gttgccgtgt
    17161 cccccgacaa aaaatacatc ggtttggcgt gggcatcgct gcaaagcggc gaattcaaaa
    17221 ccaagctgac aactgtggat aaattggacg acgaactggc gcgcctgcag gcggcggaaa
    17281 ttctgttgcc ggacagtaaa aacgcaccgc aacttcagac ggcatcgggt gttacgcgcc
    17341 tgaacgcgtg gcagtttgcc gccgacgcgg gggaaaaact gctgacggaa tatttcggct
    17401 gccaggattt gcgcggcttc ggtttggacg gcaaagaaca cgccgttgcg attggcgcgg
    17461 caggtgcact gttgaactat atccgtctga cgcaaaacct gatgccgcaa catttggacg
    17521 gcctgtcgct cgaaaccgac agccaatata tcggtatgga tgccgccacg cgccgcaatc
    17581 tcgaaatcac gcaaaccctc tccggcaaaa aatcgccgac cctgatgtcc acgctcgacc
    17641 tttgcgctac ccatatgggc agccgcctct tggctctctg gctgcaccac cctttacgca
    17701 accgcgccca catccgagcg cgccaagaag ccgttgccgc gctggaaagc caatacaaac
    17761 ccctccagtg ccgtctgaaa agcattgccg acatcgaacg catcgccgcc cgcccgcgcg
    17821 acctcgccgc cctgcgcgac agcctgtttg ccctgtccga aatcgaattg tccgccgagt
    17881 gcagcagtct cttaggaacc ctcaaagccg ttttcccgga aaacctatcc acagccgaac
    17941 agctccgcca agccattttg cccgaacctt ccgtctggct gaaagacggc aatgtcatca
    18001 accacggttt tcatcccgaa ctggacgaat tgcgccgcat tcaaaaccat ggcgacgaat
    18061 ttttgctgga tttggaagcc aaggaacgcg aacgtaccgg tttgtccaca cttaaagtcg
    18121 agttcaaccg cgttcacggc ttttacattg aattgtccaa aacccaagcc gaacaagcac
    18181 ctgccgacta ccaacgccgg caaaccctta aaaacgccga acgcttcatc acgccggaac
    18241 tgaaagcctt tgaagacaaa gtgctgactg ctcaagagca agccctcgcc ttagaaaaac
    18301 aactctttga cggcgtattg aaaaaccttc agacggcatt gccgcagctt caaaaagccg
    18361 ccaaagccgc cgccgcgctg gacgtattgt ccacattttc agccttggca aaagagcgga
    18421 acttcgtccg ccccgagttt gccgactatc cggttatcca catcgaaaac ggccgccatc
    18481 ccgttgtcga acagcaggta cgccacttca ccgccaacca caccgacctt gaccacaaac
    18541 accgcctcat gctgctcacc ggccccaata tgggcggcaa atccacctac atgcgccaag
    18601 tcgcgctgat tgttttattg gcacacaccg gctgtttcgt gcctgccgat gccgccacaa
    18661 tcgggcccat cgatcaaatc ttcacccgca tcggcgcatc ggacgacctc gcctccaacc
    18721 gctccacctt catggtcgaa atgagcgaaa ccgcctacat cctgcatcac gccaccgaac
    18781 aaagccttgt tttaatggac gaagtcggac gtggcacttc cactttcgac ggcctcgccc
    18841 tcgcgcacgc cgttgccgaa cacctgctgc aaaaaaacaa atccttcagc ctgtttgcca
    18901 cccactattt cgagctgacc tacctgcccg aagcccacgc cgccgcactc aatatgcacc
    18961 tttccgcgct cgaacaggga caggacatcg tgtttctgca ccaaatccaa cccggtcccg
    19021 ccggaaaaag ctacggcatc gccgttgcca aactcgccgg cctgcctgta cgcgcattga
    19081 aatccgccca aaagcatttg aacggactgg aaaaccaagc cgccgcgaac cgtccccaac
    19141 tggatatttt cagtaccatg ccgtctgaaa aaggagatga accgaatgtg ggcaactttg
    19201 tggacaaagc agaggaaaaa cattttgaag gtatattggc agcagccttg gaaaaactcg
    19261 atcccgacag cctgaccccg cgcgaagcat tgtcagaact gtaccgtctg aaagatttgt
    19321 gcaaatccgt atcttaattt ccgttgtcgg aacagcatca aaccatatgg aaaaatctgt
    19381 ggataaacat tatctgacaa gaagtttcca aacataaaaa tgccgtccga acagctcaga
    19441 cggcatttgt ccattcggct taaaccttat ccatatccaa acgcataacc gtaacccatt
    19501 caccgttatg gaaatgtcgc ccgacaaccg cccagccgaa tgattcataa aatatttgca
    19561 catcaggcgt ataaagatac aagaacttta tccccagcga acgcgctgcg cctatgcagt
    19621 gggcgaccag cctcctgcca atgccttttc cgcgatattc aggtaaaaca aagacatcac
    19681 ccaaccaata ttcatactgt ggaaaacttt ccatatcatg ccgcttgacc gtagccgaac
    19741 ccaacaggat tccggaatca tccacagccg caaatgccag cggcagttcg tcatccttca
    19801 aacacctgcc gtaataggca tgaatcttat ccacagaaga ccacggttca aatccgtgcc
    19861 actcctcaaa caacgcctga accaacctgc cgatatgccc ggctttcagc cgtgtaatga
    19921 aaacagtatt gtctacaaag agggaattca tcggtcaatt ccccgacgct ttcgttcccc
    19981 ctgcgccgta aaccgcattc caagcatggt ccaaacgcac tccgatttgc ctcagctctt
    20041 cagcctgccg ggctttttgc gccattgctg caggaatttc cgcttccaaa cgggcgatgt
    20101 ctgcctgagc cgcctgcaac cgtctgcgcg catcttccaa atccgactgc atcccgatga
    20161 tttttccgtc cagattgttt tgtttttgca gtaaggcacg atagccggat tggatgctga
    20221 gcagattgtc ttccgcatcc ccggcccata cgcttgtaga aaaaacaaac atcagaaaat
    20281 acaatatttt tttcatgttc aacttccgtt tgaatgccgt ctgaagccgt attccaacat
    20341 cagacggcat cacccacgcc tgtggataac ttaagcgcgg atgcgtttca acacttcctc
    20401 tttgccgatt aatgccaaca cggcatcgac gctcggggtt ttcgccgtac cgcagacggc
    20461 aaggcgcagg ggcataccga gtttgcccat tttgatacct tcttcgtcac agaagggttt
    20521 aaacaagtcg tggatggctt cggcgttcca gtcttccagc ccttcgaggc tttcggcgaa
    20581 gcgcagcatg cgtgccgctg cttcgtcatc ccagtgtttg gcaacatcgg cttcggcagg
    20641 gacttgtttt ttgtagaaat acaggcattc gtctgccaag gcgttcaaat cttgggcgcg
    20701 gtctttaacc tgtgccaaca cgtcttccaa cgcaggtttt tcggtttcat ggatatcgcg
    20761 cagagcgaga cgcggtttga cgagttcggc gagtttctcg ttgggcgtga ttttgatgtg
    20821 ttcgccgttg atccaataga gctttttcaa atccatacgg cttggggacg gggaaacgtc
    20881 tttcaaatca aaccattcga tgaattgttc catcgtaaag aactcgtcgt cgccgtgcgc
    20941 ccagcccaaa cgtgccagat agttgagcat ggcttcgggc aggatgccca tcgcgccgaa
    21001 atcggtaatg gcaacggtat cgccgctgcg tttggagatt tttttgcctt gttcgttgag
    21061 aatcatcggc agatgaccgt attcgggcag gtttgcgccg atggctttca agatattgat
    21121 ttgtttcggg gtgttgttca catggtcgtc gccccggata acgtgggtaa cgcccatgtc
    21181 gtagtcgtcc accacgacgc agaagttgta ggtcggcgtg ccgtcggcgc gggcgataat
    21241 caggtcgtcg agggcttcgt tggggataga aatttcgcct ttgaccaggt ccgcccaccg
    21301 ggttacaccg tccaaaggcg ttttgaagcg gacgacgggt tgcacgccgg cagggatttc
    21361 gggcagggtt ttgcctgctt ccggccgcca gcggcggtcg taagtcgccg tgccttcttt
    21421 ttcggctttc tcgcgcatcg cttccaattc ttctttgctg caatagcagt agtaggcatc
    21481 gccttttgcc aagagttcgg cgatgacttc tttataacgg tcgaagcggc gggtttggta
    21541 caccacgttg tcggcgttgt cgtaatcgag accgacccat tccataccgt caaggatgat
    21601 attgacagat tcggcggtgg aacgcgccaa gtcggtgtct tcgatacgca ataaaaattc
    21661 gcctttatga tggcgggcaa acgcccacga aaacaaggcg gtgcgaacgc cgccgatgtg
    21721 caggtagccg gtagggctgg gggcgaaacg ggttttgacg gtcatgatgg ctccagaatc
    21781 tttaaaacgg cttattttac tgtttttacc gtgcttgggc attaaaaatg ccgtccgaac
    21841 cctgcctgcg gataagtttc agacggtatt ttccttgttt tcaatgcttc ggcacgcgga
    21901 acagtgtatc acgcgccgcc gaccgggttc ctttgggatt gcgtccgaaa aaaggttcaa
    21961 tgaaacagcc aattgaaaaa atcccacccc catttttcca aacggtagag ggataacgca
    22021 tatccctctt gcagcataaa gatttttttc ttatttcccg catcaaagcg cgtggtcggc
    22081 gtggcagaca tataaacgcg gacacccaaa tcctccgcca tttccgccgc ccgcgccaaa
    22141 tggtagggat cgctgacaat caccacgctg gcaataccgt tggcacgcaa aaccggacgg
    22201 atgttgttca ggttttcata agtgttgcgc gaagtgtttt caaacaggat gttgcgcgcc
    22261 ggaattcctt gcttgagcgc gtaccgccgc ccgacttccg cctccgtcat ataacccttt
    22321 ttactcctgc cgccggtaaa aatgattttg cctaccctgc ggctttggta aagcgcgatg
    22381 gcgtggttga tgcgttcgcg gaaaaccggg gaagggcgtt tgtcccacgc ggcggcgccc
    22441 aacaccagcg cggcatccgc ccggacatac ggcggcaaaa cctgcccgcc cgtccgataa
    22501 accgcccaaa cggatgaggc aaacaccagc aaaagcggaa aaacactcaa acagaaaccg
    22561 cccaacaggt aatagcgcaa gccgttgcgg ctgcaaaaca gccgtttgtt cacaataccg
    22621 cttcgatatt ttccaacggc ctgccgacag ccgccttacc gttcgccaaa acaatcggac
    22681 gctccaacag ggcgggatga tcggcgatgg cacgcagcag cgcgtcattg tccaaatcgg
    22741 ggttgtccaa acccaattcc ttgtacaaat catctttcac gcgcatcatc ccgcgcgccg
    22801 atgccaaacc caatttgttg aaaatatcct tcaattcgga caagtcgggc ggcgtatcca
    22861 aatatttgac cacttcggca gcaatgccgc gttcttccaa tagggacaag gcggcacgcg
    22921 atttgctgca acgcggattg tggaaaattt tgatttcaga catgacattt ccttacttct
    22981 cgacaatccc cttattatcg gcttacacag ggttttactc aatatcccgc ctacaaccgt
    23041 accaaacggt ttacaatacc cgaatcgaca tacaaaggac aaaacgatga aatacttgaa
    23101 tcttgccgca atcacccttg ccgccacatt tgccgcacat accgcctcgg cagacgaact
    23161 ggccggatgg aaagacaaca ccccgcaaag cctgcaatca ctcaaagccc ccgtacgcat
    23221 cgtcaacctt tgggcaacct ggtgcggtcc gtgccgaaaa gagatgcctg ccatgtccaa
    23281 atggtacaaa gcgcagaaaa aaggcagcgt cgatatggtc ggcatcgcgc tcgacacatc
    23341 cgacaatatc ggcaacttcc tcaaacaaac tcctgtttcc tacccgattt ggcgttacac
    23401 cggggcgaac agccgaaact ttatgaaaac ctacggaaac actgtcggcg tactgccctt
    23461 taccgtcgtc gaagcaccga aatgcggata caggcagacc attaccggga aagtgaacga
    23521 aaaaagcctg accgacgccg tcaaactcgc ccattcaaaa tgccgttaaa cgccggatgc
    23581 cgtctgaatc cgcttcagac ggcatttttc ccgcccgacc ttcatatccg ccaaacttat
    23641 ccactatcta aaaacaggcg gaatctttat aatcggcact gtcttaccta ttgttcagac
    23701 ggcatatccc tgcggacgca accgcccgaa acgatatgcc gcccattcct tacaggacct
    23761 cctatgatcc gtttcgaaca agtttccaaa acctatcccg gcggttttga agccctgaaa
    23821 aacgtcagct tccaaatcaa caaaggcgaa atgatattta tcgcgggaca ctccggttcg
    23881 ggcaaatcca ccgtcctcaa gctgatttcg ggcatcacca agccgagcag gggcaaaatc
    23941 ctgtttaacg ggcaggatct cggcacattg tccgacaacc aaatcggctt tatgcgccaa
    24001 cacatcggca tcgtgttcca agaccacaaa atcctctacg accgcaacgt cctgcaaaac
    24061 gtcatcctgc cgcttcggat tatcggctat ccgccgcgca aagccgaaga gcgtgcccgc
    24121 atcgccatcg aaaaagtcgg cctgaaagga cgcgaactcg acgacccggt aaccctctcc
    24181 ggcggtgaac aacaacgcct gtgcatcgcc cgcgccgtcg ttcaccagcc cggcctgctg
    24241 attgccgacg aaccctccgc caacctcgac cgcgcctacg cgctcgatat tatggaattg
    24301 ttcaaaacct tccacgaagc gggaacaacc gtcatcgtcg ccgcacatga cgaaaccctg
    24361 atggcggact acggacaccg catcctgcgc ctctcgaaag gacgactcgc atgagcatca
    24421 tccactacct ctcgctgcac gtcgaatccg cgcgcaccgc gctcaaacag cttctgcgcc
    24481 aacctttcgg cacactgctt accctcataa tgctcgccgt cgccatgacc ctgccgctgt
    24541 ttatgtattt gggcatccaa agcggacaaa gcgttttagg caaactcaac gagtcgccgc
    24601 aaatcaccat ctatatggaa accgccgccg cacaaagcga cagcgatacc gtgcgcagcc
    24661 tgctgacgcg cgacaaacga ctcgacaaca tccgctttat cagcaaagaa gacggtttgg
    24721 aagaattaca gtccaatctc gaccaaaatc tgatttccat gcttgacggt aaccccctgc
    24781 cggatgtctt tatcgttacc cccgacccgg caaccactcc cgaccaaatg caggcaatct
    24841 accgagacat taccaaactg cctatggtcg aatccgcgtc tatggatacc gaatgggtgc
    24901 aaacgctgta ccaaatcaac gaattcatcc gtaaaatctt atggtttctt tccctgacgc
    24961 tggggatggc gttcgtcctt gtcgcgcaca acaccatccg actgcaaatc ctcagccgca
    25021 aagaagaaat cgaaatcacc aaactcttgg gcgcgcccgc gtcgtttatc cgccgcccat
    25081 tcctttatca agccatgtgg cagagcatcc tttccgccgc cgtcagcttg gggctttgcg
    25141 gttggctgct ctctgccgtg cgccccttgg tcgatgccat cttcaaaccc tacggactta
    25201 atatcggctg gcgtttcttc tacccgggag aaatcgcact ggtgttcggc ttcgtcatcg
    25261 cgttgggcgt attcggcgcg tggcttgcca ccacccagca cctgctcggc ttcaaagcca
    25321 aaaaataaaa caccgtcaaa aatgccgtcc gaacccgttt tcagacggca tttcaatttg
    25381 ccagtataat ggcgcatttt tccaacaagg aacctaccat gctgacctcg gaacaagtaa
    25441 aagccatgat tgaaggcgtg gcaaaatgcg aacatatcga agtagaaggc gacggacacc
    25501 attttttcgc cgtcatcgtt tcatcagaat ttgaaggcaa ggcacgcctc gcgcgccacc
    25561 gcctgattaa agacggactc aaagcccaac tggaaagtaa cgaactgcac gcactttcca
    25621 tttcggttgc cgccactccg gcagaatgga atgccaaagc acaataaccg ccacacaaaa
    25681 atgccgtcag aaacagcaca acgtttcaga cggcattttt tatatcaaac cgcttacgcg
    25741 ccgcgttttt ccaaagcagc tacggcaggc agctctttgc cttccaagaa ctcaaggaac
    25801 gcgcctccgc cggtagaaat atagccgatt tggtcggtaa cgccgaattt agcaatcgcc
    25861 gccagcgtgt cgccaccgcc tgcaatcgag aatgctttgc tttgggcaat ggcttcggca
    25921 agggctttcg taccgcctgc gaactggtca aactcaaaca cgccgaccgg cccgttccat
    25981 acgaccgtgc cggcggcttt gagcaactcc gcaagcgcgg cggcggattt cgggccgatg
    26041 tccaaaatca tctcatcttc ggcaacgtcg gcaatgtctt tcacgaccgc ttcggcatcg
    26101 gcagcaaagg ctttggcaac gacgacatcg gtcggcagcg gcacagaacc gcctttggcc
    26161 gccattttct ccatgatttt tttggattct tccaccaaat cgtgttccgc caaagatttg
    26221 ccgatggctt taccttccgc caacaggaag gtgttggcga taccgccgcc gacgatgagt
    26281 tggtcgactt tgtccgccag cgattcgagg atggtcagct tggtggacac tttgctgccg
    26341 gcaacgatgg caaccatcgg gcgcgcgggc tgtttcaagg ctttgcccaa agcgtcgagt
    26401 tcgcccgcca tcaatacgcc ggcgcaggca acgggagcgg cttgggcgac ggcttcggtc
    26461 gaggcttggg cgcggtgcgc ggtgccgaac gcgtcattga cgaacacgtc gcacaaagaa
    26521 gcgtaggctt tgcccagttc caaatcgttt ttcttctcgc ctttgttgat acgcacgttt
    26581 tgcagcatca cgacatcgcc cgcgttcaaa gccggtttgt tttcgcgcca gtcgttcaat
    26641 actttcacgt ttttgcccaa caggccgccc aaatgcgcgg caacgggcgc aacgtcgtct
    26701 tcggggtgaa actcgccttc ggtcgggcgg cccaagtggg tcatcacgat aacggatgcg
    26761 ccgttgtcca tgcagtattg gatggacgcg agcgaggcgc ggatacgggt gtcgtcgctg
    26821 attttgccgt ctttgaacgg cacgttcata tcggcgcgga tgaggacggt tttgccctgc
    26881 acgttttgtt cggtcagttt taaaaatgcc ataatcagtc cttttcaatc agtgtttgcg
    26941 atacagaaac aattgatgcc gtctgaaggc ttcagacggc atcgcaaccc gatcagccgg
    27001 atacgcgctc gattttcgcg ccgacgctgc cgagtttttt ttcaatattt tcataaccgc
    27061 gatccaagtg gtaaatctgt tcgaccacgg tttcgccgcg cgccgccaaa ccggcgataa
    27121 cgaggcttgc ggacgcacgc aaatccgtcg ccttaacgac cgcgccggaa agctgttcca
    27181 caccctgcac aaatgccgta ttgccctcgg ttgtgatgtt cgcccccatc cggttcaact
    27241 cggggacgtg cataaagcgg ttttcaaaaa tcgtttccac cacgcggcag cttccctctg
    27301 ccacggcatt caatgccata aactgcgcct gcatatccgt ggggaagccg gggtggacga
    27361 ccgtgcggat gtccaccgcc ttcggacgct gccgcatatc gatggcaatc caatcgtcgc
    27421 ccgcctcaat caccgcacct gcctcaacca gtttgtccaa caccacttcc atcgttttcg
    27481 gggcggcatt ccgcaaaacc accctgccgc cggttatcgc caccgcgcac aggaacgtcc
    27541 ccgcctcgat ccggtcgggg acgacgctgt gttcgcagcc gtgcagctcg tccacacctt
    27601 ccacgatcat ggtcgatgtt ccgatgccgc tgattttcgc gcccatttta accaggcatt
    27661 ccgccaaatc gaccacttca ggctcgatgg cgcagttttc caaaaccgtc gtaccttccg
    27721 ccagcgtcgc cgccatcagc aggttttccg tgccgccgac ggtaacgaca tccatcgcca
    27781 cgcgcgtacc tttgagttta cccttagcct tgacgtaacc gtgttcgatg acgatttccg
    27841 cccccatcgc ttccaagcct ttcaaatgct gatcgacggg gcgcgaaccg atggcgcagc
    27901 cgcccggcag gctgacttgc gcctcgccga aacgcgccag cgtcgggccc agcaccaaaa
    27961 tcgaagcgcg catcgtccga accaactcgt aaggggcgca cgtgttgttg accgtaccgc
    28021 cgttgatttc aaattcgctg atattgtcgg tcaggacgcg cgcgcccatc ccctgaagca
    28081 gtttttgcgt ggttttgaca tctgccagca tagggacgtt tttcaggcgc aacgtacccg
    28141 atgtcagcaa acccgcgcac atcagcggca atgccgcgtt tttcgcgccc gagaccgtta
    28201 tttccccgtt gagcgggccg tttgcggaga ttttcagttt gtccacgttt gttctttcct
    28261 ggtgggtact tgtaatattt caattctcgg gacaacgcat aaagcatcac ccgatgaagg
    28321 ttgcagaggc ggaattataa gggattttcg ggaaaaatac ggaagccgca ccaaagaatt
    28381 tgacgaaatg ccgcgctttc cgaacaagga ttgtcggaaa acaaaaaaag ccgagttttg
    28441 aaaactcagc tttttttgtt ttatctggtg ggtcgtgagc gattcgaacg ctcgaccaac
    28501 ggattaaaag tccgctgctc taccggctga gctaacgacc cgacaagctc gaaattctat
    28561 acatcaagcc tgatgctgtc aaccattttg tcggcgttga gacggcattt tatttatcag
    28621 gctgtttttt cgtataaatt aacgaggtca gtatcgatgc acccaacgcg ccgaacacga
    28681 ccgacagcga aacggaaatc gggatatgca cccaatgcat taccagcatt ttcacaccga
    28741 taaaactcaa cacgaatgcc aagccgtatt tcaggaagat aaagcgttcc gccacatccg
    28801 ccagcaggaa atacatcgcc cgcaaaccca aaatagcgaa aatattggag gtcagcacga
    28861 taaacggatc ggtggtaaca gcaaagacgg cgggaatact gtccacggca aacacgacat
    28921 cgctcaattc aatcatgacc agcaccaaaa acagcggcgt ggcgattttt ttgccgtttt
    28981 cgacggtaaa aaatttctcg ccgtgaaatt ccgtgccgac cggaacgact ttcttgacgg
    29041 cattcagcag cctgctgttt gccaaatcct ctttctcatc gccttcgggc ttcatcatgt
    29101 gtataccagt atagagcagg aacgcgccaa acagatacag aatccactca aactgctgaa
    29161 ccagtgccgc gccgacgaaa atcatgacag tgcgcaatac caatgcgccc aatacgccgt
    29221 acagcagcac gcggtgctga aactgtggtg cgactttgaa gtagccgaat atcatcagga
    29281 acacgaaaat attgtcgact gccaacgatt tttccaaaat gtagccggta aagaattcca
    29341 atactttttc ttttgcgact gccgcgccgt agccgagatt gccggcgagt tcgaaataca
    29401 gccagcccgc gaacaggcag gatacggcaa cccacaagcc gctccatgcc aaggcttctt
    29461 tgacgccgac tttatggctg ccgtttttct tcagcgaaaa catatccaag gcaatcatga
    29521 ccagcactgc cgcaaaaaaa acgccgtaaa acaacggcga cccgatgccg ggatattctg
    29581 tcatggttca atctcctgat ttgaaatgta attgtgttac cagctgatat aaaacatcgc
    29641 ttttgccaaa aagacaatca gcagcatatg ggtaaagacg acggcgtgta tgtatttcga
    29701 ccagccgacg gtcagtgtgg aacgcgccat tttgacgacg gcgatggcga agtgcgccaa
    29761 cacgctgaac gccaacagga ttttcagcgt cagcatcgta ccgaaggaag tggcaaacgg
    29821 ttcgcccaat atagaaagat agcggtttgc cgccatcacg acgccgctgg cgaacagcag
    29881 tccgaccaca aacggcatca ccctgacggc gcggtaagac attgcctttt ccacttcgcg
    29941 ccgcgcctcg cacgacaccc gtcccgtatg caggacggac aaaaccagca cttcaaaaaa
    30001 cacgccgccg acaaaggcaa tggcgcaata caggtggacg atgtgcgcga cggcataaat
    30061 actcatacga tgctccaacc ggaaaactcg gatacggatt gtatcactat cgtccccgat
    30121 gtccgcatac cgcttcccgt accgcctcgg cgattctcgc gcccgctccg cgatgttgtg
    30181 cgataaagcc gtccacgtgc gcctgcatct gcattccgcc cccctcggac gataaggttt
    30241 tttcaacggc ttcccgccac gcgtccgccg actcaacccg aaccgccgcc cccgatgcca
    30301 aggcgtgccg gcaggcttcg gaaaaattgt aggttgaaaa gccgaatatc gtcggaacgc
    30361 cgcaggaaag cggttcgata atgttctgac agcccgaatc gaccagactg ccgccgacaa
    30421 aagcgacatc ggcgcacagg taatacgcat acagctcgcc catactgtcg cctatccaca
    30481 cctgcgtatc aggttcgacc ggcaaaccgt cgccgcgccg ctgaacctta aacccgaagc
    30541 gttttgccgt ttcaaatacc gtctgaaaat gctcgggatg gcgcggcacg acgaccagca
    30601 gcgcatcgcc gcgatattgt tgccacgccg ccagcagttt ttccgcctcg tcttcgcccc
    30661 gataaacgcg cgtgctgccg cacacggcaa ccggtctgcc tccgatgcgt ttttcaaact
    30721 gccccgccag cgttttcatc tgttccgacg gcatcaggtc gtatttggta ttgccgcaca
    30781 cctgcacgga tgccgcgccc aatttcgcca accgcgccgc atccgcctcc gtctgcgcca
    30841 gacaacccgt cagcgaagcg gcggcaggac ggatcaggcg gcggactttc agataaccgt
    30901 tcaacgattt ttccgacagc cgcgcattcg ccaaaaacag cggcacaccc gcgcgccggc
    30961 attccttcat taggttgggc cagatttccg tttccatcaa tacgccgaac atcgggcggt
    31021 gttcgcgcaa aaactgccgt acccacgttt ttttgtcata cggaagatag cggcattgcg
    31081 catcgggaaa cagaacttgc gcggtttccc gtcccgtcgg ggtcatctgc gtcatcagca
    31141 gcggcgcatc gggaaaacgc tgccgcaact cgcgtatcaa aggttgggcg gcacgcgttt
    31201 ccccgaccga aacggcgtgt atccaaaccg cgccggtaac gggattcgga tacggcttgc
    31261 cgaaacgctc gccccgatgc gcccgatatg ccggggcact tccggagcgt ttgtccaaat
    31321 aacgccgtat ccatatcggc gcaagcagcc acaatacatc ataaagccat tggaacatct
    31381 ttctatttcc tgcaaaacaa atgccgtccg aacggttcgg acggcatttc ggcaacggaa
    31441 tcaaatatcg taggttgtcg aagcggtatc tccgcccttg cccgtccagt tggtatggaa
    31501 aaactcgccg cgcggtttgt cggtgcgttc gtaagtgtgc gcgccgaagt agtcgcgctg
    31561 tgcctgcaag aggttggcag gcagacgttc ggtcgtgtag ccgtccaaga acgtaatcgc
    31621 cgaagccatg cagggcatag ggatgccgca ttcgaccgcc ttggcaacca ccttgcgcca
    31681 cgccggcagg cagttttcca aaatattttt gaaatacgga tccgcaccca agaacaccaa
    31741 atcgggattg ttttcatagg catcgcggat attgcttaaa aatgcgctgc gaatgatgca
    31801 cccctcgcgc cacagcagcg cagtgttgcc gtagtccaaa tcccagccgt agctttcgcc
    31861 cgcttcgcgg atcagcataa agccttgtgc gtaggaaatg attttggatg caagcagggc
    31921 ctgtctcaac gcctcaaccc attcttgttt gccgccttcg acgggcgtaa cggttcgggc
    31981 gaacagcttg ccggtctgca cgcgctgttc tttgaacgac gaaacgcagc gggcgaatac
    32041 cgcttcggaa atcagcgtca acggaatacc caaatccaag gcattgatgc ccgtccattt
    32101 gcccgtgcct ttttgccctg ccgtatcgag gattttctcg accagcggtt caccgccttc
    32161 gtccttatag cccaaaattg ccgctgtgat ttcaatcaga taagaatcca gctcggtttt
    32221 gttccactcg gcaaacacgc ggtgcatttc gtcgtaagac agtcccaaac cgtctttcat
    32281 aaactggtac gcttcgcaaa tcaactgcat atcgccgtat tcgatgccgt tatgcaccat
    32341 tttgacaaaa tgccccgcgc cgtccttgcc gacccagtcg caacacggtt cgccctgcgg
    32401 cgttttggcg gcaatcgcct gaaaaatcgg tttgacggct tcccaagcgc gcttatcccc
    32461 gcccggcata atggacgggc cgtgccgcgc gccttcttcg ccgccggata cgcccgcccc
    32521 gacaaacaaa atcccttttt cggcaaggta atgtgtccgc cgtgtcgtgt cggagtaatt
    32581 ggcattgccg ccgtcaataa tgatgtcgcc ttcttccaac agcggaagca gttgttcgat
    32641 aaagtcgtca accaccgaac ctgcgcgaac catcatcatg atttttcttg gttttgccag
    32701 cttatcgacc aagtcttgca gggagtacgc gccgataata ccggtccctt ttgccgcgcc
    32761 gtttaaaaat tcgtccacct taccgattgt tcggttgtag gcgacaacct taaatccgca
    32821 atcgttcata ttcaaaatca ggttttgccc cataaccgcc aaaccgatta cgccaatatc
    32881 gcctttcatt gcaggaagct ccgttataga tttaatttat cgaccgcaac tctacctgat
    32941 ttacacttgt ttaacaatcc ttaactcttt aattttttga aaagatgcct ttacgcttta
    33001 ccgtgcgttt ccctgaaggc ttataaataa aatataaaaa ttaaataaaa aaacgatgat
    33061 tatattgata ggagaaattt tctgtgggta actttttttt attttaaaaa tcatcaggat
    33121 tacctttttt tagggtgtcg gtaaggcgga ttcccttttg tgcatacctg tggattgttt
    33181 ttcatgaaga atagtttttg tggacagttt gcttgttgtg cagatggcat cccacttttc
    33241 tttaccgaat ggctgccgat gtctttaaga accggaatgc tgtggagatt taagccggaa
    33301 gcgtgtttgg aacttgtggg aatgagaaag tgtcggcacg aatgtctttt tttctgcata
    33361 ttggcagagt gtgcatccga atttgtgtat aagtggtgga aaaaatgaga tttgcgggta
    33421 aatctcacaa tatttcagtc agataacttt ggattgcttg tgtataagta aactttcgga
    33481 tggggatacg taacggaaac ctgtaccgcg tcattcccac gaacctacat tccgtcattc
    33541 ccacgaaagt gggaatgatg aaattttgag ttttaggaat ttatcggaaa aaacagaaac
    33601 cgctctgccg tcattcccgc gcaggcggga atccaggtct gtcggtacgg aaacttatcg
    33661 ggtaaaacgg tttctttaga ttttgcgttc tggattcccg ctttcgcggg aatgacggga
    33721 ttaaagtttc aaaatttatt ctaaataact gaaactcaac gaactagatt cccactttcg
    33781 tgggaatgac ggcatcagtc tgccgtttac agcacggttt ctttagattt tacgtcctag
    33841 attcccactt tcgtgggaat gacgaatttt aggtttctga ttttggtttt ctgtttttgt
    33901 aggaatgatg aaattttaag ttttaggaat ttaccggaaa aaatagaaag cgttatccac
    33961 aagttctgat gttcagctcg tgaaatgcgt cgggcaaatc atcgctgtcg gcaaattcca
    34021 cccggtcgta agccgtttcg tctgccaaaa ccgcgcgcaa gagtgcgttg ttgatggcgt
    34081 gtcccgattt gtagccttca aacgcgccga caatcggatg tccgacgata tacaaatcac
    34141 cgatggcatc aaggattttg tggcgcacaa actcatcggg atagcgcaag ccttcaggat
    34201 tcaggacatc cgtgtcgtca atcacgatgg cgttgttcaa attgccgccc aaacccagat
    34261 tgtgggcgcg catcatttcc acttcgtgca taaagccgaa agtgcgcgcg cgcgcgattt
    34321 cgtcgatgta ggatttgccg gcgaaatcaa tttcaaaagt gggcgagctg cggttgaaaa
    34381 ccggatggtc gaattcgatg gtcagcgtta ccttaaagcc gtcatacggc gtaaagcgta
    34441 cccatttgcc cgcttctttg atttcgacag gcttgaggat tttcaaaaaa cgcttttgcg
    34501 ccttttgatc gaccacgccc gcatcttgca aaaggtaaat aaacggcagg ctggagccgt
    34561 ccataatcgg gatttcgggc gcgttcagct caatcagcgc gttgtcgata ccgtaggcgg
    34621 acagcgcgga cataatgtgt tcgatcgtgc cgacgcgcac gcctttgtcg gtaacgatgg
    34681 tggaggaaag gcgggtatcg ttgatcaaat aaggggtcag cttgatttgt tcgcccatct
    34741 cgccgtccaa atcggtgcgt cggaaggaaa tcccgctgtt ttcaggcgcg gggtgcaggg
    34801 tcagcgcgac gcgttcgccc gaatgcagcc cgactccggt aacgctgatg gatttcgcca
    34861 aagttctttg cagcataaac cgcttcctta tcaatggggt aagttttgga ataatacgat
    34921 aaaaccggaa aaacaagcta tgtttttcca tagtatttgc caatgtatcc gttttcaata
    34981 cgtaagccgc ataaaaatgt atagtggatt aacaaaaatc aagacaaggc gacgaagccg
    35041 cccccccctc ctgaaaaacg caaaaaatgc cgtccgaaaa cctttcggac ggcattttcg
    35101 cgtaaaccgt cattcccaca aggacataaa accaaaacag gaaacccaaa acagcaacct
    35161 aaaattcgtc attcccgcaa aagcgggaat ctagttcgtt gagtttcagt tatttagaat
    35221 aaattttgaa actttaatcc cgtcattccc acgaacctac attccgtcat tcccacgaaa
    35281 gtgggaatct agaatcccgg actttcagat aatctttgaa tattgctgtt gttctaaggt
    35341 ctagattccc gctttcgcgg gaatgacggc gacagggttg ctgttatagc ggattaacaa
    35401 aaaccagtac ggcgttacct caccttagct caaagagaac gattctctaa ggtgctcaag
    35461 caccgagtga atcggttccg tactatctgt actgtctgcg tcttcgtcgc cttgtcctga
    35521 tttttgttaa tccgctataa agaccgtcgg gcatctgcag ccgtcattcc cgcgcaggcg
    35581 ggaatccagt ccgcttgttc ggtaaatgag agggcggatt gcgcgcctgt cagataaacc
    35641 acgtgtttaa acgggcggca atgaggtacg cgcagagcct tgaagtgcag tcgatatatt
    35701 attttccacc aaaacggacg accccgcttg ccttgcaaac ctttaaaaat gaagccaccc
    35761 ggattaatcc gagtggccgt ggaaaatcat ttaccgcttg atttatttaa aatttatggt
    35821 ataatttgcc ttgttagctg gcagatgctg cgtcgcggca ggttgacggc aggtgcttgg
    35881 tgtcgatctt cttggtggcg tcgttgtcgg cggcaacgtc gtctttggcg ttggcgtcgt
    35941 cgcgcgtaac cggctgtccg cagaaccatt ttaccgaacc gtcttgacgc ttggcccaca
    36001 gggagagttt tttgccttgg atttctttgt ttacgccggt tgaaagcatt gtggcggtaa
    36061 tgacgccgtt tgcgacttca actttctcaa catatttgcc tttgattgtt gaagaagatg
    36121 ccacgccggc attactgttg ccgtcaggcc atttgccgtg attcaggtaa tactcggtga
    36181 cggctgattt ttgaccttca gccaaaagaa tggcttcgga aacttgtgcg cgggctgtgt
    36241 agtcttgata agcaggaagg gcgactgccg ccaaaatgcc gacgatggca atcacaatca
    36301 tcagctcgat aagggtaaaa cctttttgaa gggtgttcat aaaattactc ctaattggaa
    36361 aggaaatgct tcaagcttac gccatcggca ttatgcaatg tatttgacca tcggtatttt
    36421 gttgcgatac ctgtgtatta taaagcaaga ttggtatcaa gtttgtgttt tgaggtgaaa
    36481 atttatgcgt ttatctttat atggttgttt ttattttaca ttttctttcg tttgggcgtg
    36541 gtttgaggaa gcagggggtt accgtttttt gtcagcagtg ccgaaaattg tcagttttag
    36601 tgccgatttt cggcactttt ttattggcgt ggggtatctc tattggcatg gggcatcggg
    36661 tgtgttgatt gggtcggaat ttgagatttt tgaatttgcg cggtagcatt agggtgggtt
    36721 gggtgggaaa ttttaaattt aatttttaaa aatttccgtt ttcttggaaa gtgattgaaa
    36781 tcggcgcgtg gtgttcctgt gcaaccggca gttggatcgt cgcggcaggt ttccgtgcgg
    36841 atggcttcgt cattcccgcg caggcgggaa tctaggtctg tcggtacgga aacttatcgg
    36901 gtaaaaaggt ttctctagtt tggtgtcgat tttcttgtcg gtgccgttgg cggcggtgat
    36961 ggcggcgtcg gtggcggtgc tagcgcgctg aaccggctgt ccgcagaacc attttaccga
    37021 accgtcttga cgcttggcct acagggagag ttttttgcct ttgatttcgt tgtttacgtt
    37081 gcttgaagcc atttgggcgg taacgacgcc gtttttgact tcaacgcttt gaacatattt
    37141 gcctttgatg tcggtggagg ttgctacgcc ggcagaattg ttgttggcgg gccatatgcc
    37201 gtgattcagg taatactcgg tgacggctga tttttgacct tcagccaaaa gaatgacgaa
    37261 ttttaggttt ctgtttttgg ttttctgtcc ttgtgggaat gatgaaattt taagttttag
    37321 gaatttatcg aaaaaacaga aaccgctccg ccgtcattcc cgcgcaggcg ggaatccagg
    37381 tctgtcggta cggaaactta tcgggaaaac ggtttcttta gattttacgt cctagattcc
    37441 cactttcgtg ggaatgacgg tttaggtatt tttatagaaa gccgtaggtg gtgtttctat
    37501 gcaaacggca gatgaagcgt cgcggcaggt tgacggcagg tgcttggtgt cgatgttggc
    37561 ggtgcccttg ccgttggcgg cggtgacttc gtcgttgttg gcgccgttgg cgttggcgcg
    37621 cgcaaccggc tgtccgcaga accattttac cgaaccgtct tgacgcttgg cccacaggga
    37681 gagttttttg tctttgattt cgttgtttac gttgcttgaa gccattgtgg cggtaatgac
    37741 gcctttttca actttaaccc ctgcaacata tttgcctttg atgtcggagg cggaggttgc
    37801 cacgccggca gaactgttgt tggcgggcca ttcgccgtga ttcaggtaat actcggtgac
    37861 ggctgatttt tgacctttga ccaaaaggat cgcttcgtca ttcccgcgca ggcgggaatc
    37921 cagccttgtc ggtacggaaa cttatcggga aa
//
LOCUS       DAXHTY010000007        35842 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXHTY010000007 DAXHTY010000000
VERSION     DAXHTY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35842
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.007"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(194..265)
                     /locus_tag="ACFNMK_000317"
     tRNA            complement(194..265)
                     /locus_tag="ACFNMK_000317"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(230..232),aa:Arg,seq:cct)
     gene            complement(285..731)
                     /locus_tag="ACFNMK_000318"
     CDS             complement(285..731)
                     /locus_tag="ACFNMK_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC6363656.1"
                     /translation="MNFSFAPLFLVTLILLGVVSNNNSITISATILLLMQQTALIQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(736..1710)
                     /gene="ispB"
                     /locus_tag="ACFNMK_000319"
     CDS             complement(736..1710)
                     /gene="ispB"
                     /locus_tag="ACFNMK_000319"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFC6363657.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQIANDVRTALENADCSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1934..2242
                     /gene="rplU"
                     /locus_tag="ACFNMK_000320"
     CDS             1934..2242
                     /gene="rplU"
                     /locus_tag="ACFNMK_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC6363658.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2267..2539
                     /gene="rpmA"
                     /locus_tag="ACFNMK_000321"
     CDS             2267..2539
                     /gene="rpmA"
                     /locus_tag="ACFNMK_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC6363659.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            complement(2802..4688)
                     /gene="uvrC"
                     /locus_tag="ACFNMK_000322"
     CDS             complement(2802..4688)
                     /gene="uvrC"
                     /locus_tag="ACFNMK_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC6363660.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYHDSVREAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(4752..6914)
                     /locus_tag="ACFNMK_000323"
     CDS             complement(4752..6914)
                     /locus_tag="ACFNMK_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6363661.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHTAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRDGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTLLGDMMNALSEAQGSKAPIARMADKA
                     AAVFVPTVVGIALLTFIVTWLIKSDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDSGFDENALYRI
                     AAAVEQNAAHPLARAIVSATQVRGLEIPTAQNAQTVVGAGITAEVEGAGLVKAGKAEF
                     AELTLPKFSDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            7053..8003
                     /gene="trxB"
                     /locus_tag="ACFNMK_000324"
     CDS             7053..8003
                     /gene="trxB"
                     /locus_tag="ACFNMK_000324"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC6363662.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            8159..8527
                     /gene="rpsF"
                     /locus_tag="ACFNMK_000325"
     CDS             8159..8527
                     /gene="rpsF"
                     /locus_tag="ACFNMK_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC6363663.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            8528..8830
                     /gene="priB"
                     /locus_tag="ACFNMK_000326"
     CDS             8528..8830
                     /gene="priB"
                     /locus_tag="ACFNMK_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC6363664.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQHRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            8837..9067
                     /gene="rpsR"
                     /locus_tag="ACFNMK_000327"
     CDS             8837..9067
                     /gene="rpsR"
                     /locus_tag="ACFNMK_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC6363665.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            9084..9536
                     /gene="rplI"
                     /locus_tag="ACFNMK_000328"
     CDS             9084..9536
                     /gene="rplI"
                     /locus_tag="ACFNMK_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC6363666.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            9550..9657
                     /locus_tag="ACFNMK_000329"
     CDS             9550..9657
                     /locus_tag="ACFNMK_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFC6363667.1"
                     /translation="MPSETLPIVGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            complement(10324..11130)
                     /locus_tag="ACFNMK_000330"
     CDS             complement(10324..11130)
                     /locus_tag="ACFNMK_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6363668.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKRAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(11131..11877)
                     /gene="pssA"
                     /locus_tag="ACFNMK_000331"
     CDS             complement(11131..11877)
                     /gene="pssA"
                     /locus_tag="ACFNMK_000331"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC6363669.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            12460..12942
                     /locus_tag="ACFNMK_000332"
     CDS             12460..12942
                     /locus_tag="ACFNMK_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC6363670.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(12994..14205)
                     /locus_tag="ACFNMK_000333"
     CDS             complement(12994..14205)
                     /locus_tag="ACFNMK_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC6363671.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIV
                     LNSLLPDPKES"
     gene            14428..16866
                     /locus_tag="ACFNMK_000334"
     CDS             14428..16866
                     /locus_tag="ACFNMK_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6363672.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSSTD
                     LNGSRKILAHKDHL"
     gene            17074..18384
                     /gene="tig"
                     /locus_tag="ACFNMK_000335"
     CDS             17074..18384
                     /gene="tig"
                     /locus_tag="ACFNMK_000335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC6363673.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQVKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            18480..19094
                     /gene="clpP"
                     /locus_tag="ACFNMK_000336"
     CDS             18480..19094
                     /gene="clpP"
                     /locus_tag="ACFNMK_000336"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC6363674.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(19150..20415)
                     /gene="ispG"
                     /locus_tag="ACFNMK_000337"
                     /gene_synonym="gcpE"
     CDS             complement(19150..20415)
                     /gene="ispG"
                     /locus_tag="ACFNMK_000337"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC6363675.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKKLSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(20431..21192)
                     /gene="pilW"
                     /locus_tag="ACFNMK_000338"
     CDS             complement(20431..21192)
                     /gene="pilW"
                     /locus_tag="ACFNMK_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC6363676.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(21195..22289)
                     /gene="rlmN"
                     /locus_tag="ACFNMK_000339"
     CDS             complement(21195..22289)
                     /gene="rlmN"
                     /locus_tag="ACFNMK_000339"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC6363677.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(22433..22858)
                     /gene="ndk"
                     /locus_tag="ACFNMK_000340"
     CDS             complement(22433..22858)
                     /gene="ndk"
                     /locus_tag="ACFNMK_000340"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC6363678.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPAEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(22917..24068)
                     /gene="zapE"
                     /locus_tag="ACFNMK_000341"
     CDS             complement(22917..24068)
                     /gene="zapE"
                     /locus_tag="ACFNMK_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC6363679.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            complement(24459..25286)
                     /gene="fghA"
                     /locus_tag="ACFNMK_000342"
     CDS             complement(24459..25286)
                     /gene="fghA"
                     /locus_tag="ACFNMK_000342"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC6363680.1"
                     /translation="MKLMEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNSENQPLG
                     VIYWLSGLTCTEQNFITKSGFQLYAAEHQVIVLAPDTSPRGEQVPNDAAYDLGQGAGF
                     YLNATEQPWATNYQMYDYILNELPDLVEANFSTNGKRSIMGHSMGGHGALVLALRNRK
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGEDREKWQQYDASSLIQQGYKVQGIRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(25295..26404)
                     /locus_tag="ACFNMK_000343"
     CDS             complement(25295..26404)
                     /locus_tag="ACFNMK_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HFC6363681.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFDLMHEGKSIRAVIHY"
     gene            26552..26959
                     /locus_tag="ACFNMK_000344"
     CDS             26552..26959
                     /locus_tag="ACFNMK_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC6363682.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(27310..27624)
                     /locus_tag="ACFNMK_000345"
     CDS             complement(27310..27624)
                     /locus_tag="ACFNMK_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC6363683.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(27635..29320)
                     /gene="rpsA"
                     /locus_tag="ACFNMK_000346"
     CDS             complement(27635..29320)
                     /gene="rpsA"
                     /locus_tag="ACFNMK_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC6363684.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(29475..30131)
                     /gene="cmk"
                     /locus_tag="ACFNMK_000347"
     CDS             complement(29475..30131)
                     /gene="cmk"
                     /locus_tag="ACFNMK_000347"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC6363685.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSDSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRASLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            complement(30375..31806)
                     /locus_tag="ACFNMK_000348"
                     /pseudo
     CDS             complement(30375..31806)
                     /locus_tag="ACFNMK_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174056.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(31901..34225)
                     /locus_tag="ACFNMK_000349"
     CDS             complement(31901..34225)
                     /locus_tag="ACFNMK_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC6363686.1"
                     /translation="MRHSHYFQWLSLPLLSVAVTQQLYAQPNESLPTVELEPVVITID
                     KSGMALANRITQMPHTTKVIYEEQIQEQATGSRQLADVMAQLIPSLGVSSGTTSNFGQ
                     TMHGRQVQFLLNGVPLTGSRDISRQLNSINPNQVARIEVLSGATSIYGSGATGGLINI
                     VTKSDLEEEQFETRIGVHGSKLSSEGIGYQVGQSVAGVSENGNVLARLDVDYRTTGGA
                     FDANGKRIAPEPAQTDKQDSKSLSVNTNVDWQLDDKQNINLALTHYNDKQDTDYAPDY
                     GNRLAVLFGEKPSLNAIKGLSLSEQPKTTKSTFNINYHHDDLWGNTINTNAYYRREKG
                     RFYPFVAPFSIAKALPILQSMNSLDAYTKDLQASAYGVLQSESKADVLGARLALQRKF
                     DLAQKPVLLSYGIDYEQEKNRQGATGYDLTTFMQSNGLAFKDTGNRYTYGPDTTLKTY
                     GIFANANVALTDDLKISAGVRHQIIDSKTDGFVPPSESLLADLLNQNKVPYQAGTVNS
                     GKVKHHKTLFNLGANYSLTPNHSIFANFGQGYSLPDLQRVLRDVGTGFAVNSDNVEPI
                     SVNSYDVGWVGTFGDSHAKLTAFYNTSDKVTQFLKDYSVVVADTDERIYGAEFELNHK
                     INDSWTMGGSIAYTRGQYKDASGQYRELGAFRISPTKATAFAQYTTPNEHKLRLQLLA
                     IDGTQRAYKDSLIAKTNQNVRKTPEARISGYVVADLLGEIKLPKGQLNFGIYNLTNKK
                     YKSVFSQAAKVTYGELSSLSAQGRSYGISYTLNY"
     gene            complement(34272..35528)
                     /locus_tag="ACFNMK_000350"
     CDS             complement(34272..35528)
                     /locus_tag="ACFNMK_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6363687.1"
                     /translation="MIISQQKNRHLLTMVALLYVAHALPLYFFNVALPAILRHQGVDI
                     RVIGMLSLLYLPWAFKFLWASYVDNYYCAKIGKRKTWLFFTQIIVVVGLFGLGFLQFS
                     ESEIVLFLSISFLVSLASATQDIAIDGYTVEQFDKSSFGLASAMQSFGVAFGSMVGGA
                     GTLWLYQRFDWQMATISLATTVVLISCSLWFLKDQDNPKHATNLSKASLIRLIKRPQT
                     KWLLALIVVFRFVEAPMMAMLNPMLVDFGFSLSQIGLLFSVFGAIVGIGSALLAGVLI
                     KYIDIVRCLILFGWLRTAVYALLCLGLLYPLLGDFLPAVGLLKTGFMSLMAISVLLIL
                     SVRYLTMTALYALFMNAANPDQAGTDFTLFVCVELLVYFIGGAMSGFLVHQFGYDKLF
                     LWLAIGSGVSMLILPTLIKKFSVTDC"
     gene            complement(35528..>35842)
                     /locus_tag="ACFNMK_000351"
     CDS             complement(35528..>35842)
                     /locus_tag="ACFNMK_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363688.1"
                     /translation="DAFDDWLIAMSDLLASEQSFALIYHSRTGLTLPNNYRQIEAVWY
                     KTHKESFFKYCKGLVRIVDDETQRQALDSPAFHKAWRVPYAVVLSLTEAKAWINTKIG
                     AV"
BASE COUNT         9081 a         8788 c         9046 g         8927 t
ORIGIN      
        1 taaaacaaca gatttccaag ccggaacatc ttttacggag cctgaaaatc atttcatgat
       61 tctaccgtcc taaaggtcgg tttttcaagc aggaagaaaa agttttcaga tggcaaaaaa
      121 gccctccaac actgaaaggc cttatatcgg aaacttcccg caacacggga aacagacaaa
      181 tgaaatcgtc aaacctcgcc aacaggaatc gaacctgtat tttacgctta ggaggcatac
      241 gttctatccg ttgaactatg gcgagccgaa atgaaaagga gattttaacc ctttccgacg
      301 acaaaagaca agatgccggc cgcaatcagc ggcccgacag ggataccgcc cataaatgcc
      361 accccgataa ccgtcccgat taacagccct gtaattaaaa caggctgctg tcccatcaaa
      421 ggcacgccgc gtcccgccag ccaagccacg aaaataccga taaaaacggc ggatatcatt
      481 ttaaaattca aaaattcggc aacgggagga acctgcgcct ttcctgaaac caacggactc
      541 aaaaccccta tggtcaaaag aatgataccg agattcaacc cgtgcttctc gaccaacggg
      601 acaaactgta tcaatgccgt ctgctgcatc agcagcaata tggttgccga gatggtaatc
      661 gaattgttgt tgctgaccac ccccaacaga atcagcgtaa ccagaaacag gggagcaaaa
      721 ctgaaattca tcgcctcaag acaccctgac caaagattcc ttcgccagct gaatcatggc
      781 atccttcact tcgctgtcgg gcagggcatc caacgcggca acggcacaat cgactgcttt
      841 gcgcgcctcg cctatcgaat atgccaacgc atccgaacgg acgacataat cgtggatttt
      901 ctcaaaatag ctgcaatctg cattttccaa agcagtacgc acatcgttcg caatctgttc
      961 ggaaccctga cgcatcagat aaatcaaagg caaagtcggt tttccttccg ccaaatcgtc
     1021 gccgacgttt ttgccggttt cttcggtttc gccagaatag tccagcacat cgtcaataat
     1081 ttggaatgcc gtgccgacgt acataccgta gtctttcaac gcctgttcgt gttcggggga
     1141 agccttgccc aaaattgcgc cgacttgagc ggcagcttca aacaattttg ccgttttata
     1201 ttggataact tggatatatt gttcttcggt aatgtccgta ttgccgatgt tcatcagctg
     1261 catgacttcg ccctcggcaa taatgttggt tgcatccgcc atcacttcca aaacgcgcat
     1321 actgcccgag gcaaccatca gttgaaaggc gcgcgtgtat aaaaagtcgc caaccaacac
     1381 agccgccgca ttgccgaaca gattgtttgc cgttgcccgc ccacggcgca aatcgctttc
     1441 atcgacgaca tcgtcgtgca ggagggtgga agtgtggata aactcgacca tcgccgccag
     1501 cgaatacagt ttctcgtcat cataaccgac cgccttaccc gccaaaatcg tcataatcgg
     1561 acgcaggcgt ttgccgcccg cgctgatgat atatgtaccg atttgcgaaa tcagtgcgac
     1621 atcggattgc accgcacggt tgatgacttc attgactttg gcaaggtctt caggcagatg
     1681 tcgctggaaa tagggcagat tctcgagcat ggcagactct caggtttacg tttccgtcaa
     1741 acggcagata ccgcttgaaa gctgattcaa atagcgggca attatatcag caaagccatc
     1801 agggtacatc ttttcagacg gcatatggtt atccgaaccg cgcaaacttt gacaaaacag
     1861 gcaaataaca atacaatgcc gagttccgca caatggtgtg cggatattaa ccataaccct
     1921 tatggagttg agtatgtacg cggtcgtaaa aaccggcggc aaacagtata aagtttccgt
     1981 cggcgaaaaa ttgaaagtag aacagatacc agccgaactc gacagccaaa tcgaactgac
     2041 cgaagttttg atgattgctg acggcgaatc tgtaaaagtt ggcgcaccct ttatcgaagg
     2101 tgcaaaagta acggctaaag tagtggcaca cggtcgtggc gaaaaagtcc gcatcttcaa
     2161 aatgcgccgc cgcaaacact accaaaaacg ccaaggccac cgccaaaatt tcacccaaat
     2221 cgaaatcgtg gcaatcgcct aaccttaaaa cttaggagca ttacaaatgg caagtaaaaa
     2281 agcaggcggc agcacccgca acggtcgcga ttcagaagcc aaacgcttgg gcgttaaagc
     2341 ctacggcaac gagctgattc ccgcaggttc catcatcgta cgccaacgcg gtaccaaatt
     2401 ccacgcaggc gacaacgtag gtatgggcaa agaccacact ttgttcgcca aagttgacgg
     2461 ttatgtcgaa ttcaaaacca aaggcgcgct gaaccgtaaa actgtcagca tccgtcctta
     2521 caccggttct gaagaataat ccaaccgata cctgaagccg catcttttca cgatgcggct
     2581 ttttatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     2641 tacggaaccg attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagctaagg
     2701 cgaggcaacg ccgtactggt ttaaatttaa tccactatat atcggtatac cgacctgctg
     2761 tatcatattt tttacagacg gattttgtct ttgacagcat gctaatgcag atgattgtaa
     2821 atcgtttccg ccaatgcctt gctgatgcct tccacttttt ccaagtcctc gcggctggcg
     2881 gcaatcacgc cgcgcagacc gccgaagcgg gtgagcaggg cttggcggcg tttgctgcct
     2941 acgccgggga tgtcgcttaa ggaggaggta acgcgggctt tgtcgcgttt tttgcggtga
     3001 ccggtaatgg cgaaacggtg cgattcatcg cgtacggttt gcaatagatg caaggccggg
     3061 ctgttgggcg gcaggcggaa gacttcgccg gtaaaaggca gtatgagctc ctccataccg
     3121 gctttgcgct ccgggccttt ggcaatgccg accaaaggga tgtgcagccc gagttcttcc
     3181 cataccgata cggctacgcc gatttgccct ttgccgccgt caatcaacac ggcatccggc
     3241 catttgacgg tctcgccgtt ggcttcggcc tcctgcattt tgccgtaacg gcgcgtcaac
     3301 acttcgcgca tggcggcgta gtcgtcgccg ggtttggcgg tcgtgatgtt gtagcggcgg
     3361 tattgcgaag gctggatgtt ttgctcatcg tacacaacgc aggacgcaat agtggcttcg
     3421 ccttgtgtgt ggctgatgtc gaaacattca aggcggttga ggccgtctga atccatgccg
     3481 aggatttttg ccagttcatc aatgcggtgt tgctggctgc tttgttgcag gcggcgttgt
     3541 gcaatcgcca tttgtgcgtt ttgttccgcc attttcaacc agactttgcg ttcgcctatg
     3601 gttttggtga caaattgcat ctgcttgccg tgttcgcctt ccaaagcctc tttcagcgca
     3661 tcgggaacgg aaaagttgct aataatgatg tcgggtttgc ttttgcccaa ataatgttgg
     3721 gcgacgaagg cttcggcgta atcttgtccg tttggctcgg gatcgttttt ggtgtcgggg
     3781 aaaaagcttt tatcgcctac atgccgtccg ccgcgtatgc tgacccagtg tacgcagacg
     3841 aggccgtctg aaacggcgag cgcgagcagg tcgatgtcgt tggggttgtt cgggtttttg
     3901 ctgtcgatga actggttgct ctgcataatg ccgagtgctt ggatttggtc gcggtagcgg
     3961 gcggcttctt caaattgcag attagcggcg gcggtttgca ttttgtgttg cagggtacgc
     4021 gtcagttcgt cggttttgcc attgaggaaa gttgctgctt cacgcacgct gtcgtgataa
     4081 tcttcttcgc tgatatgacc gacacaaggt gcggtacagc gtttgatttg gtaaagcaga
     4141 caaggacggt cgcggtgctc gaatacactg tcttcgcagg tacgcagcat aaagactttt
     4201 tgcaacactt gaatactgtc gcgcacggcg ttgctgttgg gatacgggcc gaaatattga
     4261 tttggctttt tcagtgtgcc gcggtaatac gccatttgcg gatattgatg gccactgagc
     4321 atcaaataag gatagctttt gtcatcgcgg aaaagaatat tgtatttcgg cgacagagct
     4381 ttaatgaagt tgttttcgag aatcagtgct tcggcttcgg aacgcgtgat ggtggtttcg
     4441 atatgatgca cctgttttac catcaatgcg atgcgcggcg aatggtcgtt tttttggaaa
     4501 tagccggaca cgcgccgctt gaggttgacc gctttgccga cgtataagac tttgccgcct
     4561 tcgtcaaaaa aacggtacac gcccggcagc ttgggcaggt ttttgaggaa gagcgggatg
     4621 tcgaattgtt ccggagaacg gttttctgta ttcatatcaa tcatttgaaa aacggcttca
     4681 gacggcatat taccatatcg ctggaggcag ccttatccgt tcggacaagg ctgcagggcg
     4741 gttacatgct gctaatcgat ttttacccgt ttcaggcgca aggcgttgct caacacggaa
     4801 accgagcttg ccgccattgc cgcgcctgcg atgacagggt ttaaaaaacc gagcgcggca
     4861 agcggaatgc ccaaaatatt gtagaagaag gcgaaaaaca ggttttgctt gatgtttctc
     4921 aacgtcgccc gcgataccga tagggcatcg gcgagttggt tgaccgaatg ttgcatcagc
     4981 gtggcggatg cggtgtgttc ggcaacgtcc gcgccgcctt tcatcgcgaa gctgacgttg
     5041 gcggcggcga gtgcgggcgc gtcgttgatg ccgtcgccga ccatcgccac ggttttgccg
     5101 gcggctttga gtttctgtac ttcggcggct ttgtcgcgcg gactcatgtt gccgaaggcg
     5161 tgtgcgatgc ccagttgttt ggcgacatat tcgaccgtac cttggttgtc gccgctcata
     5221 atatagacat cgatattgtg ttttttcaga cggcctatgg cttcggcggt atcggctttc
     5281 aatgcgtcag caagtgcgaa tgcaccgata ggtttgttat cgactgagac tgcaacaatg
     5341 cttgcaatgt cccaaacgcc gtctgaaaat ttcggcaagg tcagttcggc aaattcggct
     5401 ttgcctgctt tcaccaatcc cgcgccttcc acttcggcag taatgcctgc gccgacaacg
     5461 gtttgcgcgt tttgtgcggt aggaatctcc aaaccgcgca cttgggtggc ggaaacgatg
     5521 gctcgggcaa gcggatgggc ggcgttttgt tcgacggcgg cggcgatgcg gtacaaagca
     5581 ttttcatcaa agccgctgtc gggaacgcaa taaacggcgg caacctgcgg cctgccttcg
     5641 gtcagcgtgc cggttttgtc caacacgacg gcatcgacgt gggcggcttc ttccattgct
     5701 gccgcgtctt taaaccaaat accgtgtttc accgccttgc ccatgccgac cataatcgcg
     5761 gcgggcgtcg ccagaccgag cgcgcacggg caggcaatca ccaaaacggc aacggcgtgc
     5821 atcagcgcaa ccgtccaatc gcttttaatc agccaagtaa caataaaagt caacagcgca
     5881 atgcccacga cagttggcac gaacaccgcc gccgccttgt ccgccatgcg cgcaatcggt
     5941 gctttgctgc cttgcgcttc ggatagcgcg ttcatcatat cgccgagcag ggtttggctg
     6001 ccgagttggg tggcgcggta aaccacgctg ccttcggtca tcagtgcacc tgccaacact
     6061 ttgccgcctg cctttttctc ttcagggttg gattcgccgg taagatggct ctcatccgcc
     6121 cagccgctgc cgctttcgat aatgccgtcg gctgcaatgc gttcaccgtg gttggcgcgg
     6181 ataaggtcgc cgatttgtac ttggtcgatg ggaagctgtt tccattcgcc atcgcgttgc
     6241 acgttcactt gggtcggtgt aagtttcaaa agcaggccca agctgttcag gctggatttt
     6301 tttgtgcggt gttccaaaaa tttgcccagc gacacaaaac caatcaccat cacgcccgct
     6361 tcaaaataca cgtgcgccat accgtgcgcc gtgtgcgggc tgaaaaacag catataaacg
     6421 gaatacaggt aaatcgagac cgtgccgatg gtaacgagta cgtccatatt cgccagcccg
     6481 cctttaatgc tcgcccacgc gcttttgtaa aatggcaccg ccagccaaag ttgcaccaca
     6541 ctcgccaatg cgaactgcca caacggcgga atcatccaat cgtgtctgcc gatcatcatc
     6601 cccgccatgc cgataaggaa cgggacgttg atggtgaaca gcagccacag ccgccagccg
     6661 atatggtgtt ctgcttcggg ttgcggcaat gtatcttccg ttttttcctt cgcgccgtaa
     6721 ccggttttct caatgatttt ggcaatgtcg gctactgagg ttttgctgtc gtcaaacact
     6781 acctgcgcct cttcgctggc gaagtttacc cccgccgatt cgacaaaatc ttttttgttc
     6841 aacacttttt caatgcgcga agcgcaggcc tggcaggtca tgccttcgat ttggaaacgg
     6901 attttttgtt gcataagcgt gattcctgct ttgatattca gacggcagtt aaggctgcgg
     6961 gatgccgtct gagcgccggt atggtcgaaa tttctcaaac gcgccgataa ttcgagtaga
     7021 atccgaaaac ttgacacagc aaaggaaaaa caatgtccca acaccgcaaa ctgattattt
     7081 tgggttccgg ccccgccgga tacaccgccg ccgtctatgc cgcgcgcgcc aatttaaacc
     7141 ccgtcattat tacaggtatc gcgcaaggcg ggcaactgat gaccaccacc gaagtggaca
     7201 actggcctgc cgatgccgac ggcgtgcaag ggccggaatt gatggcgcgg tttctcgccc
     7261 acgccgaacg tttcggaacg gaaatcattt ttgaccaaat caacgccgtc gatctgcaaa
     7321 aacgcccgtt tacactcaaa ggcgatatgg gcgagtacac ttgcgatgcc ctgattgtcg
     7381 caacaggcgc gtccgccaaa tacctcggtt tgccgagtga ggaagcgttt gcaggaaaag
     7441 gcgtttccgc ttgtgccacc tgcgacggtt tcttctacaa aaaccaagat gttgccgtag
     7501 ttggcggcgg caatacggca gttgaggaag cactctacct tgccaatatc gccaaaaccg
     7561 ttacgctgat tcaccgccgc agcgagttcc gtgccgaaaa aatcatgatt gacaaactga
     7621 tgaaacgcgt ggaagagggc aaaatcatcc tcaagctgga aagcaatctg caagaagtac
     7681 tgggcgacga tcggggcgta aacggcgcat tattaaaaaa caacgacggt tctgagcaac
     7741 aaattgccgt cagcggcatc tttatcgcca tcgggcacaa gccgaatacc gatattttca
     7801 aaggtcagtt ggaaatggac gaagccggtt acctgaaaac caaaggcggc acggcggaca
     7861 atgtcggcgc aaccaatatc gaaggcgtat gggcggcggg cgacgtaaaa gaccatacct
     7921 accgtcaggc aattaccagc gcggcttccg gctgtcaggc ggctttggac gcggaacgct
     7981 ggctgggcag ccaaaacatt tgaatgccgt ctgaaaccga aattttcggc ggaatgcaag
     8041 tttttcgcac caaatccttg attccgtcgg caaaccccaa tataatgccc gttctttgaa
     8101 ccttgccttc tgctttcgca tggcagaagg ctgtttttac catccataag gagataacat
     8161 gcgtcattac gagatcgtgt ttatcgttca tcctgatcaa agcgagcaag tgcccgctat
     8221 ggttgaacgt tacaaaacca tgattgctga agcaaacggt aagattcacc gtttggaaga
     8281 ttggggtcgc cgccaactgg cttacccgat taacaaaatc cataaagccc attacgtttt
     8341 gatgaacatc gaaaccactc ccgaagtggt tgaagagctg gaaaccgcat tccgcttcaa
     8401 tgatgccgta ttgcgccatc taaccatcaa aaccaaacac gccgttaccg aagcatcccc
     8461 tatgttgggt ggtgaaaagg ctaagaacct gttgagcggt gcgtctgaag aagcggtcgc
     8521 ccaataattg ggattcaata atcttgtttc gcttgccgcg ttaattgaaa aggttttccc
     8581 tattcgatat acgcctgccg gaatccctgt tttagatatt attttaaagc acgaatcgtg
     8641 gcaggaggaa aacgggcagc aatgccttgt ccaattggaa attccggcac ggattttagg
     8701 caggcaggcg gaagagtggc agcatcggca aggtgtatat gttcacgtcg aaggtttttt
     8761 agctcaaaaa agcagacgtt cccttatgcc gatgctcagg atacaaaata ttcaagaata
     8821 taaaggttaa acgacaatgg ctcgtcaatc attcaaacgt agaaaattct gccgtttcac
     8881 ggctgaaaaa atccaagaag tcgattacaa acaagttgat ttgctgaaag actttatctc
     8941 tgaaaacggt aaaatcattc ctgcacgcat tacagggacg aaggcattct accaacgcca
     9001 attggctgtt gccgtaaaac gcgcacgctt cctggctctg ctgccttaca ccgaccaaca
     9061 caaataattt tggagattga atcatgcaaa ttattctgtt agaaaaaatc ggcggtctgg
     9121 gcaacttggg cgacatcgta accgttaaaa acggctacgc ccgcaacttt ctaattcccg
     9181 caggtaaggc aaaacgtgcg accgaagcga atatgaaaga gtttgaagca cgccgtgccg
     9241 aattggaagc caaacaggct gaaattttgg cagatgcccg agtccgtcag gaaaaattgg
     9301 acggtcaaac cgttaccgtt gctcaaaaag ccggtgtgga cggccgcctg ttcggttccg
     9361 ttaccaatgc cgacattgct gctgcaatcg ttgctgccgg catcgaagcc gtgaaagcaa
     9421 atgtacgtct gccgaacggt cctctgaaag ccgttggcga gtacgaagtg gaagtggctt
     9481 tgcacactga tgccgttgct aaaattaccg ttgccgtcgt tgccgcaacc gagtaattga
     9541 aattatcaga tgccgtctga aaccctgccg attgtcggaa tgtggtttca gacggtattt
     9601 gttttgtttt ccgtatattc tgcaaaaatc gggatggttg tttttatagt ggattaaatt
     9661 taaaccagta cagcgtacct ggcttcaata tactcaatag gataggtctc ttcctaaccg
     9721 aaaaaataat tgtcttttcc cttgcaaata attgaaatca acagattact aaaacacagc
     9781 caaaatattc aattgacaaa aacttttctg attttgagaa atctgtgaaa atcaggggat
     9841 tatttatttt gaaaatggta tcgggcataa aatttggctg tgtttcaata tggtgctgat
     9901 aacatacttt gtttcaaaat gttcaatagg ataacttcct gattgaccga aaaataatgg
     9961 ccttttccct tgcaagcagt tgaaatcaac agattgtcaa aatacatcct aaaaatttaa
    10021 taacttatat atatgattgc aaaggttacg gcttgtatgc cggtcattat ggcggaaata
    10081 ccgtttggga ggcagccgca tcgcgtggaa tcggcacggc aggcgatgcg ccgatgcctt
    10141 cgggcgtgaa ggcggggcaa tcggcagttt ggaaaaaggc aatggaatct gtgggcagaa
    10201 gaagttaagg gtaaaagtcg ggcgcacggt caggttgtct gtccttttgt cggaacagga
    10261 gactgggcag ctttgtccta attaattgct tgtgcaaacg gtaaaaaggt tttttcgtgt
    10321 gttttaaagc aggttgtaca gcatttttcc ggcaatcaaa agcaacataa tgccgaagga
    10381 ttttttgagt ttggcagaag aaagtttgtg agcggttttg acaccgagcg gggcaaaggc
    10441 aatggttgcc gcgctgagga cggcgacggc gggcaggtaa aggaagccca gtgacccttc
    10501 gggcaatcct gcaatattca ggccgttgag cagatacgat attgcgccgg agagtgcaat
    10561 cggccaggca aggccggatg atgtgccgat ggctttatgg gcggggaagc cgcagtggat
    10621 taagaagggg acggaaagtg aaccgccgcc tatgccgacc cagctcgaca ttgtgccgaa
    10681 cagtgtggaa accgcagtca gtccgggcag tccgggcagc gggcgggatg ccgtctgagg
    10741 gtcggtatgc agtgttttga atgcgacggc ggttaaaaac aggatgaaga aaatttgaag
    10801 cccgaacgcg gggatatatt ttgcggagag tgcgcccgtg aatacgccga atatcatacc
    10861 cggcatcatc gcaaatacgg ttttccagtc gaccgcccgt tttttgtgct gccccaacat
    10921 actggaaaag gcggtgaaga ccatgacggc gaaggatgtg ccgacggcga ggtgttgcgc
    10981 gtaaggatgt tgtgccaaac cctgcaaatc aagtacccat aaaacgacag ggacaatcag
    11041 cgtgccgccg cctacgccga acagaccggc aataaaacct gccgcactgc ctacggcaag
    11101 caggattaag ataatgtccc aatgccacat ttaatccgcc tttctgtatt ttttccagaa
    11161 tcggcgtgcc gccataatgt agccggacag gctgtatccg agaaagaaca ggaagaggac
    11221 gagcgacggt tcccaagtga ccagaagcag caataagacg gcaagcagca ttccgacaaa
    11281 ggggacttgt ctgcggatgt tgatttcttt aaaactccaa aaagggattt ggacaatcat
    11341 cgacaggccg gcaaacagtg tgatgcccaa tgcccaccag tggacggcgg ggaatttttc
    11401 gacgctgtgg ttgacccaaa tcagcccgac aatcagcgcg gcggcagtcg gactgggcac
    11461 gccgataaac cagcgtttgt ccaccttgcc gatgagcgtg ttgaacaggg cgaggcgcag
    11521 ggcggcgcag gcgcagtaga tgaaggcgac ggaataaccg attttgccga actgccaaag
    11581 ctgccatttg taggcaataa gcgcgggggc aacgccgaag ctgaccatat cggcaaggct
    11641 gtcgagctgc tccccgaacg cgctttggct gttggtcagc cgcgccacgc gcccgtccat
    11701 accgtccagc aacatagaga tgaataccgc gatggcggcg gtttcataac gtccgtgcat
    11761 ggattgggtg attgcgtaaa atgcggaaaa cagcgcggcg atggtaaagg agttgggcag
    11821 caggtagata ctgttttgac gcagggagcg ttgcggggtg ttggtcgggg tttccatagc
    11881 tgaagtgtct cacggacgtg gagaatcgat aggggattat aacccaaacc attatcgaac
    11941 aatacgccct ttaggatgaa gccctcggag gcggtttgtt tttctgatgg cggatgcttt
    12001 gcggggcggt ttgactctgc ctgccaccgg gtgtgttgtt tcggattttt gccggatgga
    12061 tattgttgcc gggcatttct gggcttgccg gaaggtatcg gggggagggc atcggaatat
    12121 gcggtgcgcc gttcaaaccg gggcggcgtt tccctatttt cagggagttg caggtttcaa
    12181 caaattgaaa gatatttttt gcatttccga ccgtatgctg attttgaaac gggataaatt
    12241 ataggacaag gtttttcgcc cgccgcaaag gttggccgca atgccgtctg aaaacaacgg
    12301 cttgccgcta aggcggcggt atgcggattt gcgctgccga aatcggtttg ccatacccgg
    12361 cggtgcaatc tgcggggagg gataagccga taaggcggga cttatcctgt tttttgttta
    12421 taatgcaaca gaatttggac gaaccgtcgg gaatttatca tgatgaaaaa gatactggcc
    12481 gtatcggcac tatgcctgat gactgcggcg gcacgggctg ccgatactta cggctatctc
    12541 gccgtttggc agaatccgca gaatgcggac gatgttttgc aggttaaaac tacaaaagaa
    12601 gattcgacga aaagcgaagc gtttgccgag ttggaagcct tttgcaaagg tcaggacacg
    12661 cttgcgggca ttgccgaaga cgagccgacc ggatgccggt cggtcgtgtc gctgaacaat
    12721 acctgtgtcg cgctggcata cccgaaagcc ttgggcgcga tgcgcgttga aaacgccgtt
    12781 gtgattactt ctccgcgttt tacgagcgtt catcaggtcg cactcaacca gtgcatcaaa
    12841 aaatacggcg cacagggaca atgcggcttg gaaacagtgt attgcacgtc ttcttcttat
    12901 tacggcggaa ctgtgcgctc tttgattcaa aatctcaaat aaaacggaaa atgccgtctg
    12961 aaagatgttc agacggcatt tccatatcga cggtcaggat tctttcggat cgggcagcag
    13021 gctgttcaac acaatggcaa gtacggcgca caagcccacg ccggcaaagc tgaagctgcc
    13081 caatttgagc gtcatgccgc cgatgcccgt ggtcagtacc gagctgacga tgaccaggtt
    13141 tttcggcagc atcaaatcga ctttggcatc aatcagcgtt ttcacgccca aagaagcaat
    13201 cgtgccgaac agcagcagca taatgccacc catcacgggc atcggaatgg aagccaaaaa
    13261 cgcattgaat ttgccgaaaa acgccatgca gacggcaaaa accgccgccc aagtcatgat
    13321 gacagggtta ctgtttttgg taatcatcac cgcacccgtt acttcgccgt aggtcgtaac
    13381 cggcgggccg ccgatcagac cggcaacgca cacgcccaaa ccgtcgcccg caagggtttt
    13441 gtccaagccc gggtctttcg tatagtcttt ccccgtcaca ttgccgattg ccatgatgcc
    13501 gccgatgtgt tcgatggcgg gggcgacggc aacgggcagc ataaacagtg cagcctgcca
    13561 gttgatctga ggcgtttcaa aatggggaac ggcgaaccag ggcgcgtgtg caatgcttgc
    13621 cgtgtccacc agtcccatca gcagtgccaa aacataaccc gaagcgacac cgatcaagat
    13681 gggaatcagc ttcatcatcc tgctgccgaa aaccgatacg atggcggtaa cggcaaaggt
    13741 aaagccggaa agaatcagcg aatcggtata gtcgatgacc tgtttgccgt ccgcctgacc
    13801 cattgccatg ctgcttgccg ccacggcaac agacagaccg atgaccatga tgacggggcc
    13861 gatgacgacc ggaggcagca gtttgtgtac cgctgccagt ccgcgccaac ggatcagcgc
    13921 ggcaaacaca aaatacataa agccggcggc aaacagtccg aacatggtgg aaggcagccc
    13981 ccattcgccg acggagtaga taatcggtgc gataaaggca aacgaagaac caagaaaaat
    14041 cggcactttg cgtttggttg tgatttggaa cagcagcgtt cccaagcctg cgcccaaaag
    14101 cgcaagagcc ggattcagac cggtcagcag gggaaccagc accattgcgc cgaatgccac
    14161 aaataaaatc tgtgcaccgg aaacggcaag tttcagttgg ttcataccat cttctttcaa
    14221 tcggttcaaa cggcacggat tatactggtt tgccgagggt ctgcgtaaaa atacatatgg
    14281 cgggcaacag atatgccgtc tgaaaggcaa tgtctgtctg ttgcaggatg aaagcaggag
    14341 aaatggagta taatccgaca ctttccatat tcaatccgca agccgtccgg cttccttatt
    14401 tcagacggca ttttccggta atacggcatg acagaaaaat cccataaaaa aacagcaaaa
    14461 ggcagggcgg gaagcccgtc cccaacgtcc gcacgcaaca agaaagccga caacggcgca
    14521 cggggcaata aagtttccga gcggctcaag gcggtcaaag agttgcagaa aaccgaaacc
    14581 aaaaaggcgc gtcccgaaca tgtcgtcaac cttatcggcg acgcactgtg gctgatgggt
    14641 ttggcggcaa ccctgtattt ggcgatttcc ctgatcagtt tcgatatggg cgatccgtct
    14701 tggtcgcaca gttcgccggt tgtggaagat gtcgccaatt ggggcggact gttcggcgcg
    14761 tatgttgccg atgtcggcta ttatcttttc ggctggtcgt tctggtggtg gatagcggct
    14821 gcctgcgtcg tgctgtataa aaatttccgc ctgcacgcaa aacagacgga aaacgaggca
    14881 tacaaccaca aaatcgctgc cgccgcgctg tttgtcctga cggtcttcag ccccgtcttg
    14941 gagtattttg tgctgggcgg aaaatatgcc gactccctgc ctgtcggagc aggcggtatg
    15001 gtcggcatac gcgtcggcgc agtgtttgcg tggctgctgg ggaaatcggg cagcctgctg
    15061 attatcttgg ttgttctgct gttgtcgttg tccctgctgg tgcagatttc atggctggaa
    15121 tttttgaacg gcgcgggcag ggcggttcaa aaccgcctga gtgccttatc cggcaaggtc
    15181 atggctttag gaaaacgccg gccgaatacc aaaacagacg gtgtcgatac ccaaaataca
    15241 cggcgcatgg taaaagaagc caagaatatt acggccaaac ccgttgcctt gcccgaaggc
    15301 agcagcagca accgcaaatc cgtcgcggtt tccgtcgcgc cgccgcccaa aattcaggtt
    15361 tctctgtttg aagatgacga acctcggcag gcgggcgaat accacaagcc tacattgaac
    15421 ctattgcgga ttcctgacag cgaacccgtc agcatcaatc ccgccgaatt ggagcgcact
    15481 gccgaactga tcgaatccaa actggcagaa ttcggcatcg gcgtacaagt cgtatccgcc
    15541 acatccggcc ccgtcatcac gcgctacgaa atcgaacccg cgcaaggtgt taaaggcagc
    15601 caaattgttg ccttgtcgaa agatttggca cgctctatgt cgctgcagtc cgtgcgtatc
    15661 gtcgaaacca tcgcaggtaa aaacacgatg ggcatcgagt tgcccaacga caaacgccaa
    15721 gacgtgatgt tgagtgaaat cttgtcctcg cccgtgtttg ccgaagccaa atccaagctg
    15781 accgtcgcgc tgggcaaaga cattgccggt acccccgttg tcggcgactt ggcgaaaatg
    15841 ccgcaccttt tggtcgccgg tatgactggt tcgggcaagt ccgtcggcgt gaacggcatg
    15901 attatgtcta tgcttttcaa agctacgccc gacgaagtcc gctttatcat gatagacccg
    15961 aaaatgcttg agttgagcat ttacgacggc attccgcacc tgctctgccc ggttgtaacc
    16021 gatatgcgcg aagcagggca ggcgttgaac tggtgcgtcg ccgaaatgga aaaacgctac
    16081 cgcctgcttt cccatgccgg tgtgcgtaat ttggagggct tcaaccaaaa agtcgaagcc
    16141 gcaaaagcgg caggcaagcc gctgctcaat ccgttcagcc tgaaccccga cgagcccgag
    16201 ccgctggaaa aactgccgtt gattgtggtc gttatcgacg aacttgccga cctgatgatg
    16261 accgaacgca aagccgtcga gcagcaaatc gcccgtctcg cccaaaaagc gcgcgccgcc
    16321 ggtatccata tgattgtcgc cacccaacgt cccagtgtcg atgtcgttac cggcctgatt
    16381 aaagccaaca tcccgacgcg tatggcgttt accgtgcaaa gcaaaatcga cagccgtacc
    16441 atcctcgacc aaatgggcgc ggacgaactg ctcaaatatg gcgattcgct gttcctccag
    16501 cccggcagtg ccgaaccgac tcgcctgcaa ggcgcgtttg tttcagacga cgaagtacat
    16561 caagtcgtca actacgtcaa atcgcaagcc ccagccgact atattgaagg tctgctcagc
    16621 ggcgaggccg cgctggaaac tgccaatatc gttaatccga atgcagacag cgacgaattg
    16681 ttcgatcagg cagtcgccta tgttttggaa agcaaaaaaa cctccatttc gtctttgcag
    16741 cggcagctgc gcatcggcta taaccgcgcg gcaaacctga tggaggcact ggaaaatgcg
    16801 ggtgtcgttt cttccaccga cctcaacggc agccgtaaaa ttttggcgca caaggaccat
    16861 ttgtagcccg tattgcaaaa tgccgtctga acggcggaat tggcgtttca gacggcatat
    16921 tatgtttcag gcgaaacatt gtgatatact tgccagctaa aatttcccct ttgcggcaat
    16981 gcggttcaaa tatcgtaccg ttgcgctgtt tgcttcccca tgtagggaag aaagtttatc
    17041 attttatcaa cacaacaaat ttaagggctt atgatgagcg taactgttga aactttagaa
    17101 aatctggaac gcaaagtagt gttgtccctg ccttggtccg aaatcaacgc agaaaccgat
    17161 aaaaaactga aacaaaccca acgccgtgca aaaatcgacg gtttccgtcc gggtaaagca
    17221 cctttaaaaa tgattgccca aatgtacggt gcgagcgcac aaaacgacgt gatcaacgag
    17281 ctggtgcaac gccgcttcta cgatgttgcc gttgcccaag agttgaaagt ggcaggcttc
    17341 ccccgttttg aaggcgttga agaacaagac gataaagagt ctttcaaagt tgccgccatt
    17401 tttgaagtgt tccccgaagt cgttatcggc gatttgtctg cacaagaagt tgaaaaagta
    17461 accgcttccg tcggtgatgc cgaagtggac caaaccgtag aaatcctgcg caaacaacgc
    17521 acccgcttca accatgtcga acgcgaagcc cgaaacggcg accgcgtcat cattgacttt
    17581 gaaggcaaaa tcgacggcga accttttgcc ggcggcgcat ccaaaaacta cgccttcgta
    17641 ttgggcgcaa gtcaaatgct gcctgaattt gaagccggcg tagtcggcat gaaggctggc
    17701 gaaagtaaag acgttaccgt caatttccct gaagactacc acggtaaaga cgttgccggt
    17761 aaaactgccg tgttcaccat tacgctgaac aacgtttccg aagcgactct gcctgaagtc
    17821 gatgcagatt ttgcaaaagc cttgggtatt gcggatggcg acgttgccaa aatgcgcgaa
    17881 gaagtgcaga aaaacgtaag ccgcgaagtg gaacgccgcg taaacgaaca aaccaaagaa
    17941 tccgtaatga acgcgctgct caaagccgta gagctgaaag cacctgttgc tttggtcaat
    18001 gaagaagccg cacgcttggc aaacgaaatg aaacaaaatt ttgttaacca aggtatggct
    18061 gatgctgcca acttggatct gcctttggat atgttcaaag aacaagccga acgccgcgtg
    18121 tctttgggtc tgattttggc caaactggtt gatgaaaaca aactggaacc gactgaggag
    18181 caagtcaaag ccgttgtcgc caacttcgca gaaagctacg aagatcctca agaagtaatc
    18241 gactggtact acgcagagcc ttcccgcctg caagccccga cttctttggc gatagaaagc
    18301 aacgtcgttg atttcgtttt gggtaaagcc aaagtaaacg aaaaagcttt gtcttttgac
    18361 gaagtgatgg gcgcgcaagc ctgattatcc tgaaaatgcc gtcttagggc agtttttgaa
    18421 agcaccgaag ccgcttttaa agcccgcttt ggtgctttta catcatgaaa ggagacgaaa
    18481 tgtcttttga taactatctt gtccctaccg ttatcgagca gagcggtcgc ggtgagcgtg
    18541 cattcgatat ctattcccgg cttttgaaag agcgcatcgt attcttggtc ggcccggtaa
    18601 ccgacgagtc cgccaatctg gtggttgccc aactgttgtt tttggaaagt gagaatccgg
    18661 ataaggatat tttcttctat attaactcgc caggcggttc ggtaaccgcc ggtatgtcga
    18721 tttacgacac catgaatttt atcaagcccg atgtatcgac tttgtgcttg gggcaggcag
    18781 caagtatggg cgcgttctta ttgtcggcag gcgaaaaagg caaacgtttc gccctgccca
    18841 acagccggat tatgattcac cagcctttaa tcagcggagg cttgggcggt caggcatccg
    18901 atattgaaat tcacgcacgc gaactgttga aaatcaaaga aaaactcaac cgcctgatgg
    18961 cgaaacattg cggccgcgat ttggcagatt tggagcgcga caccgaccgt gataatttca
    19021 tgtccgccga agaggctaaa gaatatggtc tgattgatca aatcttggag aaccgtgctt
    19081 ctttacagtt ttaataagaa atctgagaga aacaaatgcc gtctgaaaac agttcagacg
    19141 gcatttgtct tatagggact gtattgggat gattttgttc tggttgcgtt tggagccgtt
    19201 ttcgccgtag ttggttttga catactcttc aacaatagcc aaaaattccg ttgcaatgtt
    19261 gtcgcctttc agtgtgactt tgcgctcccc gtccacataa accggcgcga cgggtgtttc
    19321 tcccgtaccg ggcaggctga tgccgatgtc ggccaatttg ctttctccgg gcccattgac
    19381 aacgcagccc attacggcaa cgttcaggga ttcaaccccg ggataaaggg tacgccatat
    19441 agacattttt tggcgcaggt aattttgaac atcttgtgcc agctcttgaa atacggtact
    19501 ggtggtacgc ccgcagccgg ggcaggcggt aaccatcggc gtaaacgaac gcaatcccat
    19561 agtttgtaaa atctcttgcc cgacgacgac ctcctgagta cgcgggctgc caggttccgg
    19621 agtcagtgaa atgcggatgg tgtcgccgat tccttcttga agcaagacgg ataatgccgc
    19681 cgttgatgcg acaatgcctt tgctgcccat accggcttcg gtcaaaccca aatgcagcgg
    19741 ataggcgcaa cggctgccca gttcgcggta aacctgaatc aaatcctgaa ccgcgctgac
    19801 tttgcacgac aggatgattt tgtcttcggg cagtcccaat agaacggctt tttcggcaga
    19861 ttccaaagcg gagacaatca gtgcttcctt cgtcacttct tcgggcggtt tcggcgcgga
    19921 agaagcgagg ttggcatcca tcatacgttt ggcgaggctt tgatccaaag aaccccagtt
    19981 tacgccgatg cggacggctt tatcgttttc agcagcagtc cgaatcataa aggcaaattt
    20041 ttcatcgcct tttacgcctt tgccgacatt gccgggattg atgcggtatt tggacaatgc
    20101 tttgccgcat tcgggaaatt ccgccaacag gcgttcgccg ttgaagtgga aatcgccgat
    20161 aagtggtgtg gcatagccca tatcgtccaa gcggcggcgg atttcggcaa ctttggacgc
    20221 ggcttcgggg ctgttgacgg taatacgcac catttcggat ccggcatcgc tcaatttctt
    20281 aatctgcaat gcggtggctt ttgcatcggc agtgtcggtg ttggtcatag attggataac
    20341 gacgggtgct tctgaaccga cggtaatatg atcgatgcgg acttgatgcg tcttgcggcg
    20401 ttggagtgtg ttcatatggt ttgaatctgt ttattgaccg atgaggacgg tttgcaattc
    20461 ttccgagtag gggaaattcg cctgcaattg tgcttcatat tcgtatgccg cctgtgcgtt
    20521 gccgagggct ttggcaattt tccagcctag cagcaaatca tcggcctgaa ggacttctac
    20581 cctgctttgg tattttttaa agtagtaatc ggcatcgccc aactgcccgg ccagcatttt
    20641 ggtgcgcgcc agttctttaa atgcgggtgg gaactgcggc tgggcggcga gggaacgttt
    20701 caaataggct tccgccaatc cgaattgccc ctgttttgcg ctgcatatgc ctttattcag
    20761 gttggcaata taaggggtcg ggtaggtggg gtcggccagg gctttgtcga aatatgccat
    20821 agattcggca gggcggttga gcctgccgca caggaaccag ccgtagttgt tgttgatttc
    20881 ggcactgtcg ggtttgatgg agagggcttg ccggaaactt tcctgcgcct tgtcgttaac
    20941 tttcaggtat tgatagattt cggcacggac cagccaggca agctcgtttt tagggtctga
    21001 tttcaaggcg tcttcaatac ttgccgtcgc ctgacggtag tcctgaccgc gcatatattc
    21061 cattgccaac tgggttttga tattggaaac ctgattggct ttttctgccc gcgaggggcg
    21121 gtaggaagtg ctgcacgcgc ccaaggcaag gacaagtaat aaagagattc gtttggatgg
    21181 cttaaaaggc ataattaccc ctgttgtccg attaaaatct gctgccattt ttgttggcgg
    21241 cgcgttttat cctgaacctg ccccgccaac tgtccgcagg cggcatcgat gtcgtcgccg
    21301 cgcgtttttc gtacggtaac gacaaatcct gcctgctgca aaatatcgcg gaacacacgg
    21361 atgttctcat tgctggagcg ttcgtatccg gagtttggga agggattgaa cggaatcaga
    21421 ttgaacttgc agggaacatc tgtgaccagt tcgatcagtt cgcgcgcatg ttgcgcctta
    21481 tcgtttattc cgtccaacat gacgtattcg aaagtgatga aatccctggg tgctttgacc
    21541 agatagcgtt ggcatgcggc catcaattct ttcaagggat attttttgtt caacggtacg
    21601 atttggttgc ggacttcgtc attggaagcg tggagggaaa ccgccaaagc caccggcatg
    21661 acatcgcgca acctgtccat ttggggaacc atacccgaag tggaaacggt tacgcggcgg
    21721 cggctcaaac cgtagccgtg gtcgtccagc atgatgctta aggcggtaac gacattgtcg
    21781 aagttcgcca tcggctcgcc catgcccatc atgacgacgt tggaaatcac gcgctcgttt
    21841 ttcggtgtaa cgcccatcgc tttgtttgcc caccacaatt gcccgatgat ttcggcagca
    21901 gtcaaattgc ggttgaagcc ctgccggccg gtcgaacaaa atgtacattc caaagcgcag
    21961 ccgacttgtg aggaaatgca gagcgtgccg cgatccgatt cggggatgaa gacggtttcc
    22021 accccgttgc ccgtaccgac atccaaaagc cattttcgag tgccgtctga agatttttga
    22081 gacatcatca gcttgggaat ttcgatgcct gcctgttcgt tcagtttatg gcgcaacgat
    22141 tttgccaaat cggtcatttc gtcaaaattt tgcgcgccgg attggtgcat ccaacgcata
    22201 acctgtttgg cacggaaagg tttttcgccc atatcggcaa aatgtcgggt cagcccttgg
    22261 aggtcgtagt tgagcagatt ggttttcatg tatttgtgtt tttcttaaat taaggcttca
    22321 aataaaaaat tgcagggcag gcaagtttga tttgaagcct tttgcaacag agttttcaga
    22381 cggcatggat atattttatg ccgtctgaag aggagttgtg cggtgtattg tatcaacggg
    22441 ggcagatttc ggtttggctg aaaaagtagg caatttccaa agcggcattt tccggagtgt
    22501 ccgagccgtg tacggcattg atgctgaccg aagtggcaaa gtccgcgcgt atcgtgcctt
    22561 cggcggcttc ggcgggattg gtcgccccca tcagttcgcg gtttttcaga acggcgtttt
    22621 cgccttccag tacttgaatc ataaccggac cgccggtcat aaattcaacc aatccggcgt
    22681 agaaggggcg gtctttatga accgcataaa attcttgcgc ctctttgagg gtaagctgct
    22741 tcattttggc ggcaacgatt ttcagaccgt tctcctcaaa gcggctgtat attttgccga
    22801 taacgttttt gccgacggca tcgggtttga tgatggagat ggtacgttca atcgccatgc
    22861 tatatcctta tttttactta agaagaatca aatcggtatt ctatcaaaaa aataacttat
    22921 ccgcctgaag ccttgggaga tagggtcagg tgcggctgtt ccaaataaac ttcggactgc
    22981 atttcgacca tccggctggc ggtgcgggta aattcttcgg caaaatcgcc ttccgtatag
    23041 atatggttga catctaccgc gccggtggca cacagtttga cccggaaatc gtagagtacg
    23101 tcaatcagcc aagtcagccg ccgcgcctcc gccttttctt gcggtgagag ttgttccaaa
    23161 cctgaaataa aaaccatttc ataatgttcg gccaaataca gatagtcgga ctgtgagcgg
    23221 gggccgaagc acagtgcgcg gaaatcaaac catatggcac ggccggactc ggctttgtgg
    23281 ggaatctccc gaccgtggat ggtgctgatg ccggggttca aatcggtaat gcctgtcatt
    23341 tctttgaaca gttttgccag ttttgcctca ttttcttcat tggcaggcgt aaagaaaatc
    23401 tcggcggggc ggagggtacg cagtcggtag tcttcaccgc cgtcaacgtt taagacggtc
    23461 aggctggact cgatgagcgc gattgtggga agaaaactgc tccggttttg accttgcggg
    23521 tagagttcgg aaggcgcgta gtttgaagtc gccaccaaaa caacgccctc gttaagcagg
    23581 ttttccagca gacggcctaa aatcattgca tccgcaatat cgctgacatg aaattcgtca
    23641 aaacataata cgcgggtttc tttggcaatc tcggcggcaa cggatttcaa cgggttgctt
    23701 tcgcttttca gggttttcag cctctggtgg atttctgcca taaaggcatg aaagtggacg
    23761 cggcgtttgc ggcggtaagg gaggcagccg aaaaaagcgt ccatcagaaa gcttttgccg
    23821 cgtccgaccc cgccatagaa ataaagccct ttggggactt gcggggaacg caaactcctg
    23881 cctaaaaaac ggtttctttt gcgtttgaac atcatcaatt cggtccaaag ccgatcgagg
    23941 tgttcgatgg cggctgcctg cgcgtcgtcg cggatgaagt tgggcagttg tgaggcagcc
    24001 tgataccaag tcagcgggct gtgattttca aacggcgggg ctttaaaaag ttggtctcta
    24061 ttactcatat ttaaccttgt tatacttctt gcggctaaga aagaaaatgc ttaccgtcgg
    24121 tattataaat gatttccttc aaacttggca ttttccgtgg aaaagaataa tgacgcgcac
    24181 aagggggtat aagcaaaggc ggcgtttcaa tatggtgcag atatgagctc aaatgccttt
    24241 tatagtagat taaatttaaa ccagtacagc gttgcctcgc cttgccgtac tatctgtact
    24301 gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataatcc gtagagtcgg
    24361 ctccttccgg ctcagacggc atacgtttat gccggcactt cttaccgccc tcgtgccggc
    24421 tatcccaaaa ctgctttgaa cactgaacgc tctttggttt acttcaaaaa cgcggcgtga
    24481 taagcaatat gctcgccaat aaaactggcg atgaagtaat aactatggtc gtagcctttg
    24541 tggaagcgca catcgaccgg ctggtttgcc gcacgacagg tttcgataaa atcttcggta
    24601 cgcaattgtg tcggcaaaaa ctcatcttcc aagccctgat caatgcgtat accttgcact
    24661 ttatagcctt gttgaatgag cgagctggca tcatattgct gccatttttc acgatcttcc
    24721 cctaaataag cagtaaaggc cttttctccc caaggcacga ggcttggcga taaaataggc
    24781 gaaaaagcag aaacgctttg ataacgtttc cgatttcgca gtgccaatac caatgcaccg
    24841 tgtccgccca ttgaatgccc cataatggaa cgtttgccgt tagtagagaa atttgcttca
    24901 accaaatcag gcaattcgtt caagatataa tcatacattt gataattcgt cgcccaaggc
    24961 tgctcagttg cattaagata aaagcccgca ccttgtccta aatcgtaagc cgcatcgttc
    25021 ggcacttgct ctccgcgagg gctggtatca ggggcaagca caatcacttg atgttctgct
    25081 gcataaagct gaaagcctga tttagtaatg aaattttgtt ccgtacacgt caagccggaa
    25141 agccaataaa tcacaccaag cggttgattt tccgaattat ttggcaaata gacggcaaat
    25201 ttcatttcgc attgcagcgt ttgggcatga tgcgcccaaa cttgttgcga accgccaaaa
    25261 atttgatgtt gttccatcag tttcatcgca taccttagta gtgaataacg gcgcggatcg
    25321 atttaccttc gtgcattaag tcaaaggctt cattgatttg atcgagtgtc attgtgtggg
    25381 ttacaaacgg ttctaactca atgtcgcctt tcattgaatc ttccaccatt ttcggcagtt
    25441 cagagcgacc tttcacaccg ccaaatgctg aacctttcca aacacgacct gttaccaact
    25501 ggaacggacg cgttgaaatt tcttgtcctg cacctgctac gccgatgata atggattgac
    25561 cccaaccacg gtgtgcactt tctaatgcct gacgcattac gtttacgttg ccgatacatt
    25621 caaaggtatg gtcaatgccc catttattaa tgtctaacaa cacatctttg atcggtttat
    25681 cgtaatcgtt cgggttcaaa caatccgttg caccgaactg ttttgccaac tcaaattttg
    25741 atggattggt atcaatggcg ataatgcggc cggctttggc ttgacgcgca ccttgcacca
    25801 ccgccaaacc aatcgcgcct aagccaaaca cggcaacaga gtcgccttct tgcacttttg
    25861 ccgtattatg taccgcacca atacctgtgg taacgccgca gccgagcaaa catacttgtt
    25921 catggttggc ttcagggttg attttcgcca gtgaaacttc ggcaacaacg gagtattcac
    25981 tgaaagtcga acagcccata tagtgataga ttggctgacc ttgataagaa aaacgcgtcg
    26041 tgccgtccgg cattaagcct ttaccttgtg tatcacgcac tgagacgcac aagttggttt
    26101 tacctgaaca acaaaactca cattcgccac attcggcggt gtaaagcgga atcacgtgat
    26161 cacccggttt tacgcttgac acaccttcgc ccacagcaac gaccacaccc gcaccttcgt
    26221 gtccaagcac cacagggaat acgccttcag gatcgcttcc tgataacgta aacgcatcag
    26281 tatggcacac gccagtgtgg gtattgcgga ttaacacctc gcctttacgc ggcatttcta
    26341 cgtcgatttc cacaatttgt aagggttggt taggggcgaa tgccaccgcc gcacgagatt
    26401 tgatggttga atcggcttgt ttcatttcca ttgtctttcc tcttaattca ttgacaaagt
    26461 tgccttgcct gagcaacata gcaacagttt acactttaga gttaactcta aagcaagtgt
    26521 tttttactgt ttttgtttag aaggagggaa aatgacttat actactgcca aagccgccga
    26581 aaaaataggc atctccgccc acaccctacg tttttacgac aaagaaggtt tgttgcccaa
    26641 tatcggacgt gatgaatacg gtaaccgctg ttttaccgat aacgatttgc aatggttggg
    26701 cttattgcaa tgcttgaaaa atacgggaat gagcttaaaa gacatcaaac gctttgcgga
    26761 atgtaccgtc attggcgacg ataccattga agaacgcctt tccttgtttg aaaatcaaat
    26821 agaaaatgtg aagtgtcaaa ttgccgaatt aaaacgctat ttagatttgc ttgaatacaa
    26881 attggcgttt taccaaaaag cgaaagcatt aggctcggta aaagctgtaa atttgccgca
    26941 aattcctgaa acggcttagt tttagtgcaa taagcaggat ttttgtcctg ttcgtaatag
    27001 aaatcaaaaa tttgttgttt aaaaagcgga ttgtaagcaa tgatatagtg gattaacaaa
    27061 aatcaggaca aggcgacgaa gccgcagaca gtgtaaatag tacggaaccg attcacttgg
    27121 tgcttcagca ccttagagaa ttgttctctt tgagctaagg cgaggcaacg ccgtactggt
    27181 ttttgttaat ccactataaa aaaatccacg ctctaatccg ttgcttgctt ggctcaaggt
    27241 attgaagcaa cggatgaagt taaaccacag aaaaaacgcc gcgtaacagc ggcgttttga
    27301 tgctaaggtt taattggcat tttcttttaa agccaagtcg acccgctcgc gcaattcttt
    27361 accgggcttg aagtggggta catgtttttc aggtacttcc acacgctcgc cggttttggg
    27421 attgcgaccg atgcgggcag gacgatggtt caaatcgaag ctgccgaaac cgcggatttc
    27481 gatgcgttga cctcgggcaa gcgatctagt catggtgtca accaagactt ttacgctgta
    27541 ctctacgtct tttgccagaa gatgcgtgcc gtttttggcg gcaaacactt ctgccaaacg
    27601 aaccattaac tcagactttg tcatgtctgc aaccttattc ttgttcgccg gagagtttgg
    27661 ctttcagcag gtcgcccaag ctggtggtgc cggcattcgc attggcggcg gcattgacgg
    27721 agttcagtgc ttcgcggctt tctttggcat ctttggcttt aacggaaagt ttgatgctgc
    27781 ggtttttgcg gtcaacggta acgatgacgg cttcaacttc gtcgccttct ttcagtttgg
    27841 tggtcaaatc ttcaacgcgg tcggctgcaa attcggaagc aggcaggtag ccttctactt
    27901 cgtcagacag ggcgataaca gcacctttgg cgtcaacaga tttcacggaa cctttaacca
    27961 aagaaccttt gtcgttcacg ctgatgaagt tgccgaacgg atcgccttcc agttgtttga
    28021 tacccaagga gatgcgttct ttttccacgt cgattgccaa tacgacggct tcgacttctt
    28081 cgcctttttt gtatttgcgt acggcttctt cgccggattc ggtccaggac aggtcggaca
    28141 ggtgaaccag accgtcgatg ccgccgggca ggccgacgaa tacgccgaaa tcggtaatgg
    28201 atttaaccgc accggagatt ttgtcgcctt tgttgtggtt ggcggcaaat tcttcccaag
    28261 gattggcttg gcattgtttc atacccaaag agatacggcg gcggccttcg tcgatttcca
    28321 aaatcatgac ttcgacttcg tcgcccagtt gtacgacttt gctcgggtgt acgtttttgt
    28381 tggtccagtc catttcggag acgtgtacca aaccttcgat gccttgttcg atttcgacga
    28441 atgcgccgta gtcggtcagg ttggatactt tgccgaacag gcgggtgcct tgcggataac
    28501 ggcgggtcag accgctccaa ggatcttcgc ccagttgttt catacccaag gaaacgcgtt
    28561 gtttttcttg gtcgaatttc aatactttgg cttcaacttc ctgaccgact tccaagactt
    28621 cgctcgggtg tttcacgcgc cgccatgcca aatcggtgat gtgcaacaga ccgtcgatgc
    28681 cgcccaagtc aacgaatgca ccgtaatcgg taatgttttt aacgatgcct ttgatgacgg
    28741 agccttcttg caggttttcc agcagggctt tgcgttcttc acccaaagtg gcttccagaa
    28801 cggcgcggcg ggaaacaacg acgttgttgc gttttttgtc cagtttgatc actttgaatt
    28861 cgatctcttt gccttcgaag tgagaagtgt cttttacagg acgtacgtcg accaaagaac
    28921 ccggcaggaa tgcgcggatg ctgctaatca taacggtcag gccgcctttg actttgccgt
    28981 tgatgatgcc ggacaggatg ttgccgtttt ccatggcttc ttccagggca atccaatcgg
    29041 cagcgcgttt ggctttttcg cgggacagtt tggtttcgcc gaagccgttt tcgacggatt
    29101 cgatggtaac ggtaacgaag tcgccgactt taacttcaat ttcgccttga gcgtttttga
    29161 attcagctac atcaatcagg gattctgatt tcagacctgc gtttacggta acgaagtttt
    29221 ggtcgattgc cactacttca gcggtaatca cctcacccgg gttcatttct tgcagggtaa
    29281 agctttcttc caacagctga gcaaaatttt ccatagacat atataactct tttcggtaca
    29341 ccgccaaggg gtgcggggtg ggttggtgga tgtctgccgt ccttggcagg gcagacgggt
    29401 aaataaggtt tcagacggca tatggggaaa ttcatgctgt ctgaaacgca atgcgggcga
    29461 ttatacctga aaatttaaac tttacgatac caatcaagca ctttttttac agtttcttct
    29521 atagtcaggc ggctcgtatc caaaagcagg gcatcgggtt gttgtttcag gggggcaacc
    29581 ttgcggtttc ggtctgcctc gtctctggct tcgatgtcgg acaggatgcg ctcgaatgcc
    29641 aaaccttcgc aggggatgcc gatttgtttg gcgcggcgtt cggcacggat tttggattct
    29701 gccgtcagga agattttaag ttcggcttgg gggaagacga ccgatccggt gtcccgtccg
    29761 tcggcgacca gtcctttttc ggtcagaaaa tcgcgttggc gttgcagcag ggaggcgcgg
    29821 actttaggca actgtgcgac tgcggatgcg cccatgccga tggcttctgt ccggatgccg
    29881 tctgaaacgt cttcgccgcc gagcaggatg cggctgtctg aaaatacggc gggcagtttt
    29941 tttgccagtt cggaaacgtt ttcttcatcg tgccatccta cgccttgttt ttgtgcatat
    30001 agggcggtca ggcggtagag tgcgccggta tcgagataat cgtatcccaa tgcggcggca
    30061 acgcgggcgg caaccgtgcc tttgcccgat gcgcccgggc cgtcgatggc gatgactttt
    30121 tgtctgttca taagggggat tcctgatggt ttggggtatg ggttttgccg tctgaaggat
    30181 gtatttccca ttggggcgga ttctacctgt tttaaaggac gattgtctaa gcagacggaa
    30241 cgccgcctgc ccggaaacat ccgacagaag tcggcaagcc ggttcggatt tcggcgttgc
    30301 gggtattgtg gcgggcatca aggcaatgct gtctgaaaga ggtatgacct tcagacggca
    30361 tcgattgctg cggattagaa caggttgctg acgaaaatca gcagaatcac cagcggcaca
    30421 agatatttca cataagcaaa ccaaatattg accgtcgtat ggttgccttt ataaagcaat
    30481 tcgtccttcg cttcgtcctt catcacaaaa ccggcaaaca gcgcggaacc gagcgcggtc
    30541 agcataaaca agatgttgcc gctgatgtag tcgaaggcat cgaaaatatt tttgccgaac
    30601 acggaacgtc tttccacgga ccatagctca gaatggacgg gatgttgccg aaaatgaaga
    30661 tggcagccaa tacaatcgta atcgcggcgg tacggcggat tttggttttt tcctgaatgg
    30721 tcgtaatcaa cacttcataa atggtcagcg aagttgtcaa cgcggcaatc aggagcagcg
    30781 agaagaaaat cacggcgaac acagatcccg cccacatatg tgagaacaca atcggcaagc
    30841 tttggaacac caaagtcggg ccggaatcgg gggcaacgcc gaagctgaag agcgacggga
    30901 aaatcataaa gcccgcaagt atggcgatga tggtattggt aattgccgtg ataactgccg
    30961 tctgaaccag attttcgttt ttatccaaat agctggacaa ggtaatcatc acgccgaaac
    31021 ccaagctcag ggcaaaaaat acctgcccca aaacgaagac gaacagttcg acggtaatct
    31081 tgctgaaatc aggtttcaga tagaaagcaa ccccttccat tgcgcccgga agggtaacgt
    31141 tgcggacgac catcgcgatt aggaacaaaa acagcagcgg catcagctat tttgccgctt
    31201 tttcaatgcc gccgataacg cctttgacca aaatccattg gttcacggcg acaaaaagca
    31261 gcgtataaaa cgcaatttcc caagggctgt ttcaatgtgt tcggcaaaga agccttttgt
    31321 aaccacaccg tcgacggggc tggaaatatt caaatttcct ccaataatat taacgatata
    31381 gctgattacc cagccgccga gtaccatgta ataagccatg atgccgaacg cgccgagcag
    31441 ccccatccag ccgaccagtt tccaaatttt ggcaatgggt ttgccgttca tcgggccgcc
    31501 gaacgcatcc agcgcgttca cgcctttgcg ccgtccgatg acattttcca ccaaaatcat
    31561 cgggatgccg ataaccagca tcgcgataca gaataaaaac acatacgcgc caccaccgtt
    31621 ttcaccgacc aaatacggga aacgccacgt cgcgccgaaa ccgacagtcg cgccggcaat
    31681 ggtcaggata aaggcttgat ggtgggtaag tttgtcatca tcggtgacgg atttggcttt
    31741 gttggcgatg accaagccca aatggtgctg gcgagatagg ggctgtaagg tggggtgtcg
    31801 tttcatgggg gttcctgttg gtaaattaaa ttttattata aaataaaaaa aaccacaaac
    31861 actagggcga tgtttgtggt attgtggcac ataatgctga tcaataattt aaggtataac
    31921 ttatcccata acttctcccc tgtgcagaca agctggataa ttcaccataa gtcactttgg
    31981 cagcttgact aaatacactt ttatattttt tatttgtcaa gttataaatc ccaaagttta
    32041 actgaccttt aggtagtttg atctcaccca ataaatccgc cacgacatag cctgaaattc
    32101 tagcctccgg ggttttgcgg acattttgat tggttttggc aatcaggctg tccttgtaag
    32161 ctctttgtgt accatcaatg gcaagtagtt gtaagcgtaa cttatgttcg tttggcgtag
    32221 tatattgagc aaaagcggtg gctttggtag ggctgatacg aaacgcccca agttctcgat
    32281 attgaccgct tgcatctttg tattgccccc gtgtataggc aatactaccg cccatggtcc
    32341 agctatcgtt aattttatgg tttaattcaa actctgctcc atagatacgt tcgtcagtat
    32401 cggcaacaac cacgctataa tcttttaaaa actgagtgac tttgtctgat gtattataaa
    32461 atgctgtcag tttagcatga ctatcgccaa atgttcccac ccaacccaca tcataactat
    32521 tgactgaaat tggctcaaca ttatcagaat taaccgcaaa acccgtccca acatctctta
    32581 gcaccctttg taaatcaggt aaactatatc cttgcccaaa attggcaaaa atactgtgat
    32641 ttggagttaa tgagtaattt gctccaagat taaataaggt tttgtgatgc ttcactttgc
    32701 cagaattgac agttcctgct tgatacggca ctttattctg gtttaataaa tcggctaata
    32761 gcgactcact gggtggcaca aaaccatctg ttttactgtc tataatttga tgccgtacac
    32821 ctgccgatat ttttaaatca tctgtcaaag caacattagc attggcaaat atgccgtagg
    32881 tttttaaggt ggtgtcaggt ccataagtgt aacggttgcc agtgtcttta aatgccaagc
    32941 cgttgctttg cataaaggta gtcaaatcat agcctgtcgc cccttggcgg tttttttctt
    33001 gttcataatc gataccataa ctaagtagta caggtttttg agcaaggtca aattttcttt
    33061 gcaaagccag tcttgctccc aatacatctg ccttggattc ggattgtaac accccatagg
    33121 cgctagcttg tagatccttg gtataagcat ccaaagaatt catgctttgt aaaataggca
    33181 gggctttggc gatcgaaaac ggggcaacaa agggataaaa tctgcctttc tctctgcgat
    33241 aataagcatt ggtattgatg gtgttacccc acaaatcatc atgatgatag ttgatattaa
    33301 aggtgctttt ggtggttttt ggctgttctg ataatgataa gcctttgatg gcatttaatg
    33361 aaggcttttc tccaaacaac accgcaaggc gattaccata atcaggtgca taatcggtat
    33421 cttgtttgtc gttataatgc gtcaatgcca gattgatatt ttgcttgtcg tcaagttgcc
    33481 aatcaacatt tgtattgaca cttaggcttt tgctgtcttg cttatcagtt tgggcaggct
    33541 caggagcgat gcgtttaccg ttagcatcaa atgcccctcc tgtggtgcga tagtcgacat
    33601 caagtcgtgc aaggacatta ccattttcgc tgacacctgc tacactctga cctacctgat
    33661 aaccgatacc ttcactggat aatttactac catgtacacc gatgcgggtt tcaaattgct
    33721 cctcttccaa atcagactta gtaacgatat taatcaaacc gcctgttgct ccagacccat
    33781 aaatactggt tgctcctgat aaaacttcaa ttctagcaac ttgattggga ttgatactat
    33841 taagctgtct agagatgtct cgcgaacctg tcaaaggcac gccatttaac aaaaattgca
    33901 cttgacgacc gtgcatggtt tgcccaaagt tactggtagt gccactactt acccccaaac
    33961 ttggaatgag ctgtgccatc acatcggcaa gctgtcgaga gcctgttgct tgctcttgaa
    34021 tttgctcttc ataaataact ttggtggtat ggggcatttg cgtgatacga ttggcaagtg
    34081 ccataccgct cttatcaatg gtaataacca caggctctaa ttcaaccgtt ggtaatgact
    34141 cattgggttg agcgtacaac tgctgagtta ctgccacact tagtaaaggc aaagataacc
    34201 attgaaaata gtgggaatga cgcataaatt actccttatg gaaaagtaaa attttaacag
    34261 tctgttacgg tttaacagtc tgttacggaa aattttttta tcaatgtcgg caaaataagc
    34321 attgacaccc cagaaccaat cgcaagccac aaaaatagct tgtcatagcc aaactgatgt
    34381 actaaaaatc cactcatcgc cccgccgata aaatacacaa gcaactccac gcacacaaac
    34441 aaagtaaagt ccgtgccagc ttggtcggga ttggcagcat tcataaacag agcatataag
    34501 gcagtcattg ttaaatagcg aacagataaa attagcaaaa cactaattgc catcaagctc
    34561 ataaaccctg tttttaataa acctaccgca ggtaaaaaat cacccaataa aggatatagc
    34621 aatcctaaac acaacaaagc ataaactgcc gttcttagcc atccgaataa tataagacaa
    34681 cgtacaatgt cgatatattt gattaatacc cctgccaaaa gtgctgaacc aatcccaaca
    34741 atcgctccaa ataccgaaaa caaaagccca atctgagaca gtgaaaaacc aaaatccaca
    34801 agcattggat ttagcattgc catcatcgga gcttccacaa agcgaaacac cacaatcaaa
    34861 gctagtaacc atttagtttg cgggcgtttg ataagcctaa taagacttgc tttggaaaga
    34921 ttggtagcat gtttgggatt gtcttgatct tttaagaacc atagtgaaca cgaaataagc
    34981 acaacagttg ttgccaaact gattgttgcc atttgccaat caaatctctg atatagccac
    35041 agtgtgccag caccgcctac catactacca aacgccactc caaaactttg catggcactt
    35101 gccagcccaa aggatgactt atcaaattgt tccactgtat agccatcaat agcgatatct
    35161 tgggtggcac ttgccaaaga caccaaaaaa cttatgctca gaaataatac aatctcagac
    35221 tcagaaaact gtaaaaagcc aagcccaaac agcccgacta ccacgataat ttgagtaaaa
    35281 aataaccaag ttttgcgttt gccgattttg gcacaataat aattgtccac ataacttgcc
    35341 cacagaaatt taaacgccca cggcaaatat aaaagtgaaa gcatgccaat caccctaata
    35401 tcaacgcctt gatgtcttaa aatggcaggc aatgccacat taaaaaaata caaaggcaag
    35461 gcatgggcaa catataacag tgccaccatc gtcaaaagat ggcgattttt ttgttgtgag
    35521 ataatcatca gaccgcccct attttagtgt ttatccatgc tttggcttca gtcagtgaca
    35581 acaccaccgc ataaggcact cgccatgctt tatgaaaagc aggactgtct aaggcttgtc
    35641 tttgagtctc atcatcaaca attctgacta atcccttgca atatttaaag aacgattctt
    35701 tgtgcgtctt gtaccaaacc gcctcaattt ggcggtagtt attagggagt gtcagccctg
    35761 tccgactatg gtaaatcaag gcaaaactct gctcactggc taacaaatca ctcatagcaa
    35821 tcaaccaatc atcaaatgca tc
//
LOCUS       DAXHTY010000008        34634 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXHTY010000008 DAXHTY010000000
VERSION     DAXHTY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34634
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.008"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            1..111
                     /locus_tag="ACFNMK_000352"
                     /pseudo
     CDS             1..111
                     /locus_tag="ACFNMK_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            517..1572
                     /locus_tag="ACFNMK_000353"
     CDS             517..1572
                     /locus_tag="ACFNMK_000353"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6363689.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPVKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            1586..3232
                     /locus_tag="ACFNMK_000354"
     CDS             1586..3232
                     /locus_tag="ACFNMK_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HFC6363690.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIRRSKGIYQPEFRKESVESNIESGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLNVDFIDNDDEDLIATDRTSLDWKHSKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIEDWKNKLPKKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            3236..3658
                     /locus_tag="ACFNMK_000355"
     CDS             3236..3658
                     /locus_tag="ACFNMK_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC6363691.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEILVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAIGRERLNRLVREIKDAV"
     gene            complement(3681..6923)
                     /locus_tag="ACFNMK_000356"
     CDS             complement(3681..6923)
                     /locus_tag="ACFNMK_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC6363692.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLAYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMCEWRELHHQLAQT
                     AIEMGLTTKETAFRRPPEIRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAG
                     YEQIHRALLTGLIANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TKLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYNERLPEMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSR
                     QNENHGNTVGWVENPTSAATAKTVGFDNPTYATQQPTPSPAREGRGEGKTVAAQTNFS
                     ATAANPLPQEREQSASASTFSDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATST
                     LSDDPKPKKQPAPPKGRLKPLPLADIRTFQAWIKTAERDNLRLLFLSRDDLMQHAAAH
                     ITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRLHAPSLEWLVPGMLR
                     EKIQLLIKALPKQIRRICVPVPDFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRILE
                     QINQDEWAAFRLPEHCYFNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNTQEF
                     ERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTSPAAEQAHRLG
                     VIELMKLQLKEQVKYLNKGIQGFTQAAMLLKHINADTLCDDLTQAVCDRAFIGEDELP
                     RNEKAFKEQIKRARSRLPAVKEALSRYLQETAAVYAELNSKLGKHPLTHLLRLRLQTL
                     LAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDS
                     LIKQGLPISDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLKMWEDLN"
     gene            complement(6989..8566)
                     /gene="lpt3"
                     /locus_tag="ACFNMK_000357"
     CDS             complement(6989..8566)
                     /gene="lpt3"
                     /locus_tag="ACFNMK_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC6363693.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKATRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGG
                     AGASMLDKLWLPALWGMLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQSAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTQLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLSIRDGKAEYVYPQ"
     gene            complement(8744..9871)
                     /locus_tag="ACFNMK_000358"
     CDS             complement(8744..9871)
                     /locus_tag="ACFNMK_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFC6363694.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            complement(9972..11363)
                     /gene="hrpA"
                     /locus_tag="ACFNMK_000359"
     CDS             complement(9972..11363)
                     /gene="hrpA"
                     /locus_tag="ACFNMK_000359"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC6363695.1"
                     /translation="MFPDLSQTLSKDRHFLRSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAASPKPEFDNTLPVHEKLEEIKKAIAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            complement(11553..12962)
                     /locus_tag="ACFNMK_000360"
     CDS             complement(11553..12962)
                     /locus_tag="ACFNMK_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6363696.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            complement(13022..14242)
                     /gene="argJ"
                     /locus_tag="ACFNMK_000361"
     CDS             complement(13022..14242)
                     /gene="argJ"
                     /locus_tag="ACFNMK_000361"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC6363697.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVVD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQSAATVYTCD
                     LSHGYVSINADYRS"
     gene            complement(14309..15001)
                     /locus_tag="ACFNMK_000362"
     CDS             complement(14309..15001)
                     /locus_tag="ACFNMK_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC6363698.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            complement(15001..15345)
                     /locus_tag="ACFNMK_000363"
     CDS             complement(15001..15345)
                     /locus_tag="ACFNMK_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC6363699.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            complement(15490..15708)
                     /locus_tag="ACFNMK_000364"
     CDS             complement(15490..15708)
                     /locus_tag="ACFNMK_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363700.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            complement(15727..16482)
                     /locus_tag="ACFNMK_000365"
     CDS             complement(15727..16482)
                     /locus_tag="ACFNMK_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6363701.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            16727..17635
                     /gene="hslO"
                     /locus_tag="ACFNMK_000366"
     CDS             16727..17635
                     /gene="hslO"
                     /locus_tag="ACFNMK_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC6363702.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENEWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIGEDESLVDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            complement(17721..19175)
                     /gene="mgtE"
                     /locus_tag="ACFNMK_000367"
     CDS             complement(17721..19175)
                     /gene="mgtE"
                     /locus_tag="ACFNMK_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC6363703.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(19407..23882)
                     /gene="mspA"
                     /locus_tag="ACFNMK_000368"
                     /gene_synonym="ausI"
     CDS             complement(19407..23882)
                     /gene="mspA"
                     /locus_tag="ACFNMK_000368"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HFC6363704.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIVRNDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLIHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPTALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGDSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSKFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKSENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMKNSTSWQFIGNNRQQAAKQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNGPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPAPTSHGMPTA
                     PKTGNFSDMTGEAAIEKNRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYNLSGIDLGFTQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPAT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPLKLEGDSRGAFQI
                     HVKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNP
                     LKEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARI
                     DSQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFETELD
                     TYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNL
                     AELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHR
                     PYQQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENG
                     ALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSN
                     GNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSALS
                     VNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            complement(24196..24954)
                     /gene="gloB"
                     /locus_tag="ACFNMK_000369"
     CDS             complement(24196..24954)
                     /gene="gloB"
                     /locus_tag="ACFNMK_000369"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC6363705.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGEGLITVW
                     ATPGHTDRHISYLMETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            complement(25049..29011)
                     /gene="purL"
                     /locus_tag="ACFNMK_000370"
     CDS             complement(25049..29011)
                     /gene="purL"
                     /locus_tag="ACFNMK_000370"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC6363706.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGRLNDDEKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPIDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGADKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALHDQ
                     FAAFFTDPNTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG
                     "
     gene            complement(29168..29380)
                     /locus_tag="ACFNMK_000371"
     CDS             complement(29168..29380)
                     /locus_tag="ACFNMK_000371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363707.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRTETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            29475..29813
                     /locus_tag="ACFNMK_000372"
     CDS             29475..29813
                     /locus_tag="ACFNMK_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC6363708.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVACSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            complement(29879..30756)
                     /locus_tag="ACFNMK_000373"
                     /pseudo
     CDS             complement(29879..30756)
                     /locus_tag="ACFNMK_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
     gene            complement(31049..31807)
                     /locus_tag="ACFNMK_000374"
     CDS             complement(31049..31807)
                     /locus_tag="ACFNMK_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6363709.1"
                     /translation="MITIRNVSYRIGTRPILDNISLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKAVLTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(31891..32517)
                     /locus_tag="ACFNMK_000375"
     CDS             complement(31891..32517)
                     /locus_tag="ACFNMK_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC6363710.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAQDGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGIQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSINAALKPFAPE
                     MRELMPKAVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            complement(32562..33536)
                     /locus_tag="ACFNMK_000376"
     CDS             complement(32562..33536)
                     /locus_tag="ACFNMK_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC6363711.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCGLFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFALGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            complement(33526..34494)
                     /locus_tag="ACFNMK_000377"
     CDS             complement(33526..34494)
                     /locus_tag="ACFNMK_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6363712.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFVAY
                     EFEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAILWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAYAV"
BASE COUNT         7471 a         8215 c        10344 g         8604 t
ORIGIN      
        1 aaactgatta accgacaact gaccgaaaaa aacctaaaaa tagagaaagc atccgctgcc
       61 gtcgttgacg ccaccattat tcagaccgcc ggcagcaaac agcgccaggc ccgataacgc
      121 ccggcggaag tttttcggac atcgcgaacc ttgccctgct gaaaatacaa aaggccgcgt
      181 tcggcgatat ggggcgggaa caggtgagga gggtggatac ggggcatggt tggcttccaa
      241 gaagttgttg aaatatgggg agtttactgg ttcgggcgac gtttggggag ggggtgggaa
      301 ttcttgtttt gggaagtgga aaaaggtcgt ctgaaaatgg gtttcagacg acctttctgt
      361 gaagtgtgca tttgaagaca tccgctcgtt tggaggaaat taagttctgc ttatttagat
      421 gcatgaattt taataaaatc tcaaattatt tttgataagc aataaattat atgttatata
      481 aagttagttc tcactagtct aattatttaa atagaaatga aaaacagtaa gttaaaggcg
      541 gttgattttt tctgtggcgg aggtggaatg agctatggaa tgcaatccgc cggtattcag
      601 gttttagccg ggatagatta cgaacccagc tgcaaagaaa catatgaagc caatataaat
      661 gccaaattta tacaggctga tgtttttgaa ttgcagcctg aaaccttgga aaaagaactc
      721 gggttaaaaa agaatgatga tgatttgatt cttataggtt gtagtccttg tcaatattgg
      781 agtgttattc aaacagacaa aagaaaatcg gaaaaatcca agagtttgct tttggagttt
      841 cagcggtttg tagaatattt caatcccgga tatgttgttg tagagaacgt accgggaatt
      901 ctgagcagaa tgaaagaaag caggcttgat aatttcataa agttattgga agaaaaagga
      961 tttaccgtcc atttcggtat tcacaatacg gctgattacg gaattcccca aagccgtaaa
     1021 agatttacgt taattgcaaa cagaataacc aaagaaaagc tggaaccagt caagtattcg
     1081 ggcaaacggc ttacggtacg cgatgttttg ggaatggaaa atggctttcc caaaattatt
     1141 gcaggacacc aagacgaaac ggattttatg catagctgtg cgggattatc tgatatcaac
     1201 ttgaaacgat tggctttgat acctaaaaac ggaggaaacc gtttggcttt tgcgcatatt
     1261 cccgaattac agctggaatg ttttatcgga aaagacaata gttttaaaga cacgtttggt
     1321 aggctatggt gggataagcc cgcacctacc ataacgacca aattctttag tatttccaac
     1381 gggaggtttg cccatcctga agaagatagg gcgttatcac tgcgcgaagg tgcaacattg
     1441 caatcgttcc ctcgtaacta tgtctttaaa gcgggcagca gggacaagat agcccgtttg
     1501 attggtaatg ccgttcctcc gatgtatgcg gaaaaaatag gcagggcaat tgttgataat
     1561 atcgaatgtt agaaaaggag aaaacatgtc tgaagaaaaa ttgaaaatga gtttcgagcc
     1621 aaccgtaatc gaacatttgg gtgtaaagat gtattcgcac actgttcctg cgattgccga
     1681 gttgatagcg aatgcctacg atgcatgtgc tacggaagtg gaagttaggt tattcgataa
     1741 accggagcat aaaatcgtta ttaaagataa tggtatagga atgagcttcg atgaaatcaa
     1801 tgatttttat ttgagaatcg gtcggaacag aagggaagaa aaacaagctt ccccgtgcgg
     1861 aagaattcca acgggtaaaa aaggccttgg taaattggca ttattcgggc ttggcaacaa
     1921 aattgaaatt tctactatcc agggaaacga aagggttact tttactttgg attatgcaga
     1981 gattcgaaga agcaagggta tttatcaacc ggagtttcga aaagaatctg ttgaatccaa
     2041 tatcgaaagc gggacaacca taactttaac cgaactgacg aaaaagcaag gatatccgtt
     2101 agataattat gtagagcatc tttcccgctt gtttgatttt ccggctcagg attttaaaat
     2161 caaagtaagc ttgaacggct ctgaacctaa aatcattgat ggaaatctaa aatatgatct
     2221 tgttacccca caattcgaat gggaatacca ggatttagca accaatattt catcgttatc
     2281 ttcaaaattc gaacagtatg aatacagcgg attaatacaa ggtaagttca ttacaacgga
     2341 aaaaccttta aagaataata tgaaaggtat taccttgttt gccaacggca gaatggtaaa
     2401 tatgcccgag tttttcactg atagcgaatc cagccatttc tattcatatt tgactggatg
     2461 gctgaatgtg gactttatag ataatgacga tgaagattta attgctaccg acaggacatc
     2521 attagactgg aagcattcaa aaacctcaaa attacgtgtt ttccttgggg agatcgtccg
     2581 agccatcggg caagattgga gaaaaagaag aaaaaaagag aaagatgacg aagtgaaagg
     2641 cgaaagcgga gttgatatcg aagattggaa aaacaaattg ccaaaaaagg aaagggaacc
     2701 agtagaagtt attttgaatc gtttggaaga ttcggagctt acaaataaag agcaagcgga
     2761 agtaatctct gccttacata gtattattcc agaatatcct tactatcatt ggcggcattt
     2821 gcatcaggat ttgcatactg cctgtaatga tttttacaat gaaaagaagg attatttaag
     2881 tgctgcaatc gaggcagtta aagttttcga agataaggtt cagaagcaaa caggtcttca
     2941 tagcatagac ggaagagaat tgattgaaca agctttcggg agtaaaaatt ctatacttct
     3001 attaacgaat aacaaaacga aggcggaaca aaatttagaa gatggtcttg aacagcttgc
     3061 atgcggaaca tggactggat ttagaaatcc tgtacaacat gagttaagag caaatttatc
     3121 tcctagtatt tttaatgata aggatgcttt ggatttaata agcttggttt cgtatttgtt
     3181 aagaaaagtg gaacagacaa aaaaacgatc gaaggtggta tcttccaagt aatttatgac
     3241 cgatattttc actacatcca aacgttcttt cgtgatgtca aaaatccata gcaaagaaac
     3301 caaaccggaa atattggtaa ggaaatttct tttctcccaa ggttttcgtt atcggaaaaa
     3361 cgataaacgc tatgcaggca aaccggatat cgttttgccg aaatataaaa cagtcgtgtt
     3421 tatacacggc tgtttttggt acgggcattc ttgtaataaa ggacatatcc cgaaaagcaa
     3481 tacggatttt tggttggaaa aaatcacaaa aaattgcgaa cgtgatatta aaaatgaaac
     3541 cgagctggaa aagatcggtt tcaaagtgat tgtcgtttgg gagtgcgaat taaaaaataa
     3601 agctattggt agggagcgat taaatcggtt ggtcagggaa ataaaagatg ccgtctgaaa
     3661 aaatcagacg gcatcttcct ttaattaaga tcctcccaca tcttcaacaa ccgcttcacc
     3721 gacaccggat acggtgtctt caattcctgc gcgaacagcg acaccctcaa ttcttcaatc
     3781 atccatttaa acgcggcgag gccgtctgaa atggggagac cttgtttaat caggctgtct
     3841 gttttttcct gccacatttg ttccagctct tggatatcgg cttcgcgggc tgcgtcgcgg
     3901 gcggggttgc tgctgtattt ttcgaggcgc agggtcatgg ctttgaggta gatggggagg
     3961 cgcggccatt gtgcccacgg ggtgcgggtg gcgaagccgg cggcgagcag ggtttgcagg
     4021 cgtagtctta gaaggtgggt caatgggtgt ttgccgagtt tgctgttgag ttcggcgtag
     4081 acggcggcgg tttcctgcaa gtagcggctg agggcttctt tgacggcggg caggcggctg
     4141 cgggcgcgtt tgatttgttc tttgaaggct ttttcgttgc gcggcagctc gtcttcgccg
     4201 ataaaggcgc ggtcgcagac ggcttgggtg aggtcgtcac acagggtgtc ggcgttgatg
     4261 tgtttgagca gcatggcggc ttgggtgaag ccttggatgc ctttgttcag gtatttaact
     4321 tgttctttta gttgtagttt catgagttcg atcacgccta atcggtgggc ttgttcggcg
     4381 gcgggggatg tgtcgaacag gcgcagggcg atgcggccgt ctttttcttt ttgcaggccg
     4441 aggtagccgg tgagctgttg tttgccgcgt gcgaatttga tggattcggg cagggtgcca
     4501 atgtcccatg tggtcacgtt gtcgcgctcg aattcttggg tgttgtcgcg gaaggtaacg
     4561 gcggcggctt ggccgagttg ttgttgcaat tcatgcagtt tgcggccgcc ggcaagctct
     4621 tgtccgccgt cgtcgataat gcggaggttg aaatagcagt gttcgggcag cctgaacgcg
     4681 gcccattcgt cttggttgat ttgctcgagt atgcggatgt cgcctgcggt tttggcgatg
     4741 gcttgggcaa gttggggcag gatgggggcg ttgcgatcag ggttttggct taaaaattgg
     4801 gtgatgaaat cgggtacggg cacgcaaatg cggcggattt gcttcggcag agctttaatc
     4861 agcagctgga ttttttcgcg caacataccg ggcaccagcc attcgagcga cggcgcgtgc
     4921 aggcggttga ggacggtcag cggtagggtg agggtaacgc cgtcgagcgg atggtgcggc
     4981 tcgaagcggt aggaaagttt gaacttgccg tctgcggttt gccagaattt ggggaattgc
     5041 tcttcggtaa tgtgtgcggc ggcgtgttgc atcaaatcgt cgcggctgag gaacagcagg
     5101 cgcagattgt cgcgttcggc ggttttgatc caggcttgga aggtacggat gtcggcgagg
     5161 gggaggggtt tcagacggcc ttttgggggt gcaggctgct ttttgggttt ggggtcgtct
     5221 gaaagtgttg aggttgcgga aaagttggtt tgggtggcaa ctgttttgcc ctctccccag
     5281 ccctccccca cgggggaggg ggcggtttgc tgcagatttg caggacgcag gtcgtctgaa
     5341 aacgttgaag ctgaggcact ctgttccctc tcctgcggga gagggtttgc tgcggttgcg
     5401 gaaaagttgg tttgtgcggc aactgttttg ccctctcccc gaccctccct cgcgggggag
     5461 ggggtgggtt gctgagtagc gtaggttggg ttgtcaaacc caacagtttt tgcagttgcg
     5521 gctgatgttg ggttttcaac ccaacctacg gtgttgccgt gattttcatt ttggcgagac
     5581 ccattacgct tgtttttacg aaactcgttg cgctcatttt cagacgacct ctcggctttg
     5641 tcagattcaa taatccgtac ggaatcttca cttccccaaa cgctgccttg cgcgtctttc
     5701 caagccattt cgggcagtcg ttcgttataa aacgcaaaca gggcttcgtc atcgaccagc
     5761 acgtcttgct tgcgcgattt gtgttcgagt tcggtaattt ctttaatcag ctttttgttg
     5821 tggacaaaaa aatccgcttt caaatcgcat tcctgcgcca ccaacgcgct gcggataaag
     5881 atttcgcgcg cttcttcggg ggcaactttg ccgtaagaca cggggcggcg cggcaatacg
     5941 gtcagaccgt aaagcgtcac gcgttcgctg gcgacgactt cgccgcgttt ttgttcccag
     6001 tgcggctcga aatagtggta gcggacgagg tgcggggctt cctgctcaat ccattcgggc
     6061 tggataacgg cgacgtcgcg cgcataaagt tttgtcgttt caacgagttc tgccgccatc
     6121 acccatttgg gtttggcttt gaacagggcg gaggcgggga aaaggtggaa gcggctgccg
     6181 cgcgcgccgg tgtagtcgtt gccgtcgggc gatttcatgc cgacgttggc gataaggccg
     6241 gtcagcaggg cgcggtggat ttgttcgtag cccgcttctt tggcggcgcg gatttgggcg
     6301 cggtgttgct ttttatccag ttgtttttgt ttgagcttgg cggataagtc ttggtcaccc
     6361 gcattttcag acgacgtgag ctgcctgatt tcgggaggtc gtctgaaagc ggtttccttg
     6421 gtggtcaaac ccatttcaat cgcggtttgg gcaagctggt ggtgcagctc gcgccattcg
     6481 cacatacgca ggtgcgacag aaaatattgg cggcaccact gcaccagctg cttgttggac
     6541 aaacctttgt cgcgctcgcg ctgaaagctg tcccaaatat tcagataggc aagaaagtca
     6601 gattgcttgt cggtaaaacg ctcgtgcgcc ttggccgcgg catcacgcgc ttccagcggc
     6661 cgctcgcgcg ggtcttgaat cgacagcgcg gacgcaatca ccaatatttc cgccatgcag
     6721 tcgtgtttct tcgccgccag caagatgcgt gcgattttcg ggtcgatggg caggcgcgcc
     6781 atttgttcgc cgagtttggt caggcggtaa cgcgtgttgg taccgatatg acccgaactg
     6841 ttttgcaggc cgtctgaaac attggccgga ttggatcgtg catccatgcc tgatccgcgt
     6901 acttggtttt tcatattttg catagtcttg tcggtcgggc atcttccccg acatcatcta
     6961 aatttgtttt tattggtttt cacgtcgctt attgcggata aacatattcc gccttgccat
     7021 cgcgaatgct caagctgcct gcatcgcccg taatcaggtt gcccgttacc gagccttcgc
     7081 gacaacctga aaccggcata tcgtagccca acgtgtgaat caggaacgtt gaaagctgct
     7141 gatggaaggc aatctcgcaa ggtgcaaaag cctggttggc agcctgttgc acggccttat
     7201 ccgggctgta caacaccagc ggcacgagat agctgtcggg ctgcaccgtg ccttgattgt
     7261 agatatcttg gcgaacatac tggccatgat cggaggtata ggcaaacagc cagttgccgt
     7321 caggctgctt ttgcagctgc tcgaatacgg tttgaatcat ttggtcggtt ttgtggatgg
     7381 tgttgtcgta cttatccaca atatcggctt cgccgaatac tttatcttga ggctgcaaca
     7441 atgcgccgta tggggcgtgc gaaccgcgtt ggtgcaacac gataaaatgc ctgccctgct
     7501 gcaaattgat tttgtcgaac agcggcagca gcttctcatc gggcatattg tcgccgttgc
     7561 cgtagccaag ctgcgtcggt tgaatcagat ggtctatcca tttcttaccg attaagttca
     7621 aaattgccat ttggttttca gcctgagcac tgtaaaaata cgtctcatag ccctgctctt
     7681 tggcgaggcg gaacatattg gtatcgccgc cgctgatttg ttccaagccg ttggcatgcg
     7741 gtatgacgtt aaagaaactg ggcagggata ctgccgtcat aaagcctgcg gaataacttt
     7801 gtttcacaat cggcttaaaa tcggcttgcg acagctgggt taaaaacggc gaagtttcgc
     7861 gcccgtagcc aaacaatttc aaatgcgccg cgctttcgct ctcgcccata atcaggacga
     7921 tattttgaac actgccctgc ccgattttgc ttggcgcaga ctgtttgaac acagggattt
     7981 tgcttaaatc aaataactga tacggcaata cgcgtccgac aaaataaccg aagctgaaat
     8041 aattggcttt gatacggctg tatgtcggtt tgggcgaaat accgtgctct tgtttcgtgt
     8101 cgaacgaacg cacgaaaatc atcagcatta ggaaggcaaa cagtatatcg gcagaaaaat
     8161 gcgtcttacg gcggaacttg gcaaggctgc aaaacaacat gacttccaac atgccccaca
     8221 acgcaggcag ccacaactta tccaacatcg acgcccccgc gccgccaact tcggtaatct
     8281 ctttcagcat cagccaataa ttgatgcccg tcatccaact ttgataaacc gcgtaatgca
     8341 cattgttggc aataatgctg aacgcgaaaa acaccgcaat caacaaacgc gtcgccttat
     8401 aacgcgcaaa cagatacagc gcagcaatca caaatgtcag cgcaaacgtt tctgccattt
     8461 ttgcagccgg taaggtttca attccgaata caaagcgata agcaatttcg ctggcagtaa
     8521 gtagggacga atacagaaag agaacgaaaa gggatttttt cataatggga agttcacaat
     8581 aaaagttgca ggacgggttt tggggctcgg caacggtatt cttcagcagc tatctgttta
     8641 cagattgttt ttcaaaaaac gaggttgtct gaaaagggtt tgtggagttg attaagggtg
     8701 gtaggttgtt tttggttttg taggtcaggc ataaatgccc atcctacttg atatacacaa
     8761 ttttagtacc gtcaatacgt aggacttggg tatcttgtaa atgttgtagc agtttgtcag
     8821 tttcttgtgc attggatttt gtgagtgcct ggattgtatt gcgtaaagtt ttggttttat
     8881 taggacggtt ctttaacgat aacgcggcaa tcactttttt ccaaataatt tcatcaggtt
     8941 cccatgcggt ttttttaggc tgcttgggcg ttggctgtgg tttaggttca gtaaacggtg
     9001 catattcaat tttcccattg ttttggcgga tcaggttttg gtcgcgcaaa tgtcgcgcaa
     9061 aggattgaat gtcgttggta ccgactgtta agtgaagtgc atctgctcgg ttttgcacga
     9121 cggcttgaaa atcagcgtag gttttgggac gttggcttaa aatatggcgt tggattcttt
     9181 gtaacaaatc attatcactg agatggtagg aaacggtttc ctgttcatca atgctgatga
     9241 agttttgtgc tttgagttta ttgataacgt tagcagtggt tatttcgcat tcttcatcgg
     9301 ttttttcttt aaacaaatca ctcaaaatat gcttacgcag attttgtcgc aggttatgca
     9361 ggcggcaggg gcggaaagcg tcggggcggc gcagtgcagt tagggctgct tggaaatagg
     9421 aagttaatgg ttgctgtggt ttgagggtgg tttcaggctg aggagtgttt tccagcaatg
     9481 ctgacggcgg ttcggtagta attttctgat gttgtacttc atctatattg gctaggcgca
     9541 cgataccctt cgcctggtgg tttttcaaaa tatgttcgac cagaacatcg tatcctccat
     9601 cacgcgagag tatgccgatt tgggcattgg ggtctgtggc ggtaatttgt ccgaggtaat
     9661 aggacaggta aaaatccaat gcgtttttcc ctgttttttg taactggaca agatggacac
     9721 gttcgccgaa acgtagtagg gattgcacca gactaatagg taacattttg tgtaatacac
     9781 ctataaatag ccaaatatgg gtattttcgg ttagtaattt gtctaagttt tgcggctgga
     9841 cgttttcaaa atcgatgagt aagtgtttca tgtttttacc gtgtgcaatg ttgtaggttg
     9901 ggcatctaga tgtgccgtag ttttatcgtt tgttggtctt tgtcaggtag aagtgtatat
     9961 cctacttgtt ttcaggcggc ctccactgcc cctaattcca gcaacacctg aaacccatca
    10021 ttaatatacc gcgaatcggg catttctaaa aatgggaatg ccgctacgtc gccgagtttc
    10081 agcgatgcca tgcgcaggat gacggcggcg aggttgctgc gggcgatttc ggggtcggta
    10141 aattcggggc ggttgttgaa atcttcttct gaaaacaatc ggatacatac gcctgcggag
    10201 acgcgtccgc agcggccgga gcgttggcgg gcggcggctt gggagatttt ttcgatatga
    10261 agctgctcca ctttcgcccg tgcggaatag cgtttgacgc gtgcgaggcc ggtgtcgatg
    10321 acgtatttga tgcccggcac ggtaagcgag gtttcggcga cgttggttgc cagcacgatg
    10381 cggcgtttcg cgcctgaggg gtggaagatt ttgtgctgct cggcgtgcga caggcgtgcg
    10441 aacaggggca ggatttcgtc gttgcggcgt agcgtggatt tgcgcagggc ttcggcggct
    10501 tcgcggattt cgcgctcgcc cggcaggaat accaagatat cgccttcgcc gtgtcgcgcc
    10561 aattcgtcgg ctgcatcgac aatcgcgtcg gtcagctcga cttctgcatc gtcttcgtct
    10621 ttgccggtca gcggtcggta gaggatttcg acgggatagg tgcgtccgct cacttccagc
    10681 acgggcgcgc cgttgaagtg ttgggagaag cgttctgcgt ctatggttgc cgaggtgatg
    10741 atgactttca aatcggggcg gcgcggcagc agttgtttca aatagcccaa aaggaagtcg
    10801 atgttcaggc tgcgctcgtg cgcttcgtca atgataatcg tgtcgtaggc ggcgagataa
    10861 cggtcggtct gcgtttccgc cagcaggatg ccgtcggtca tcagcttgac gcaggcatcg
    10921 cgcgaggtgt ggtcggtgaa gcgtacttta tagccgaccg cgctgccgat ttcggatttc
    10981 agctcttcgg caatccgctc tgccacggag cgcgcggcca aacggcgcgg ctgggtatgc
    11041 ccgatcagcc ccgccgcccc acgcccgagt tctaaacaaa tcttgggcag ctgcgtggtt
    11101 ttgcccgaac cggtttcgcc gcaaataatc gttacctgat gctcggcaat ggcttttttg
    11161 atttcttcga gcttctcgtg aacgggcagg gtgttgtcga attcgggttt gggcgaggct
    11221 gccaaacgtt tcaaaaagat ttcgtgcgat tttcggtatt tttcttcgac tttggacaag
    11281 ccgccgtatt tgttgggatt tttgaaggca gaacgcagga agtggcggtc tttggagagg
    11341 gtttgggaga ggtcgggaaa catgggtgtg cgtgtgcggt aaaaagtgca attatagcaa
    11401 acgcttcggt tttgtttagg caatatcggg atggggaaat gccgtctgaa aaccgcattc
    11461 tgtcgttcag acggcattgt tgctgaaatt tcaagcggcg gcggaacggt attcgaatca
    11521 aggcggctgc cggcgtttga tcggggggcg ttttaatttt cagacggcat tcccgttttg
    11581 ctgtttgctg tttgcggtct tgcgggcgca ttgccggttt gggcggcggt ttcttgaagc
    11641 acgcgctggc ggaagcgcat tcccgatgcg tggtagaacg gacgtggcga aaactggcgc
    11701 gaaacctgcg aggcgaaaat gcaggcaatc agcatccaaa acagcaggct ttgtccgcca
    11761 gtcatttcca tgacgacgac ggcggaagta atcggggatt gtgtcgcgcc cgccagaaat
    11821 gccgccatgc agatgaggac gatgatgttt gcaccctgcg atatgtcggc gatggcggcg
    11881 atatgctcgc ccaaaaccgc gcctatggtc agcgagggag tgaaaatgcc gcccggaatg
    11941 cctgcccaat agctgaatac ggtggcgagc catttggcgg cggcgagtcc gaagggggct
    12001 tcgtagatgc cgtgcagggc ttgggcggct tcgtggtagc cggtgccgta ggttttgcct
    12061 tggtagaacg tgccgagcag ggcgagcagc agccccatca gtgccgccag cagcagcgga
    12121 cggttgcgga tgaagccgcg tatcttgcgc ggtgcaaacg ccgccgcacc gcgatagagc
    12181 aaacgtccga acaggccgcc cgccgcgccg caaaccaggc cggacagtgc gacccacaga
    12241 aagatatgtt ccaatacgcc gccgttgaaa cccgaaaaat acgggttgtt gccctgaatg
    12301 gcgacctgta tgaaaccgga ggcgagcacg cccaaaagaa tttgcctctc ccagcgcaac
    12361 atgatgccgc gcccgagttc ctcaatggcg aaaatcacgc ctgccagcgg cgcgttgaac
    12421 gcggctgcca aaccgcccgc cgcgcccgcc gccatcaaat cgttttcctg catccctttg
    12481 aatgccaagc cgtgtttctt gcaccacgcg ccccacgcgc ccatcactgc cgcgccgacc
    12541 tgcacggacg gaccttcgcg tccgatggac gcgccgaaca gcatacccaa aaaggttagc
    12601 ggaatcttca gcagcgtctg cccgaggcgg atcagccgcg ttttctgtgc gccgtagggc
    12661 agcgacagtg aggcgatgac ctgcgggatg ccgctgccgg cggtgaaggg ggcgaatttg
    12721 cgtgtgagcc acgcaataag cggcaaaccc aaaggaagcg cgacccacgc gaaccacggg
    12781 tattgttgaa ccagtttggc gttcagttcc agcgcaaaat cggcaagatg ggcaaaaaac
    12841 agcgcggtca gggcgacgag tgccgaacct gccaaaagga acagaaaggc gatgcttttg
    12901 cgcgacaggc ggcgggtttg gcggattttg cgggcgaggc ggcgggggaa tgtggaggtc
    12961 atggcatgtt gtccggcggt tcagacggca ttttatgtat gccgtctgaa gccgtgtcgg
    13021 gtcaggaacg gtagtcggcg ttgatggaaa cgtatccgtg cgacaggtcg caggtataga
    13081 cggtggcggc actttgtccg cgatgcagct tgatgcggac ggtgatttcg gccttcgaca
    13141 tcaccgcctg cccttgtgct tcggtgtagc ttgcggcgcg tccaccgtgt tcggcaacca
    13201 aaatatcgtc gagatacatt tccacgaggt cagtatcgag gtcgacaacg ccggcataac
    13261 cgatggcggc gagcagcctg ccgaggttgg ggtcggaggc gaaaaaggcg gttttgacca
    13321 gcggcgaacg tgccacggcg taggcggctt ggcgggcttc gtcgcgggtt ttggcgtttt
    13381 cgacgcggac ggtgatgaac ttggtcgcac cttcgccgtc gcggacgatg gcttgggcga
    13441 gttcgagcgc gaggctgcac aacaattctt tgagttgggc gtaacgcggg tcggcgatgt
    13501 tgtcgatttc gctttggctg tttttgccgg tggcgatgat gacgaagctg tcgttggtgc
    13561 tggtgtcgcc gtcaacggtg atggtgttga aggtttcgtc ggcgatttcc tgcgtcatca
    13621 gttggaggac gggttgggaa actttggcat cggtggcgat gaaaccgagc atggtcgcca
    13681 tattgggatg aatcatgccc gagcctttgg cgatgcccgt ggcgcggacg gtgtgtttgt
    13741 cgccgacctt gccttcgcgc gaggcggctt tgggcacggt gtcggtggtc atgatggcgc
    13801 gtgccgcttc gttccagaag gcaggctgca ttttgggcag ggcggcgatg attttgtctg
    13861 cgggcagcgg ttcgagaatc acgccggtgg agaagggcag cacctggttc ggtttgcagc
    13921 cgatttgccg ggcggcggcg gcacacactg ccaaagcatc gattctgccc tgtgcgcccg
    13981 tacccgcgtt ggcgttgccc gtgttgatga cgagggcgcg cacgccgtct tcgtcgaaaa
    14041 ggtgcgattt ggcgatgtgg acgggcgcgg cacagaaacg gttggtcgtg aagactgcac
    14101 cgacggtgct gccggcggct acggcaatca gtgtcaggtc ggtatgcccg ggcttcttca
    14161 cgcctgcttg ggcggtgtag agggcaatgc cgtcgatgtc gggcagttgt tcggcggttt
    14221 tttcggtcag gttgacagcc ataacggttc tccttgcgag tgtgtcggcg ttcagacagc
    14281 atgggctgaa atgccgtctg aaacgggttc aaaatcccaa aacggggatg agcagcggcg
    14341 caatcagcgc ggtcagtacg ccgttgaacg tcagccccag tcccgcgtat gccgccatgc
    14401 ggcggctgcg ttcgagcgag gcggcaatcc ccatcgcgtg cgaagccgtg ccgagcgaca
    14461 tacccacgga cgagggcatg acgactgtgt tcttcagcat tttgtaaccg gcaatctgtc
    14521 cgaccagacc ggcaatgatg acggtggcgg cggtaatggc gggaatgccg ccgatggagc
    14581 gggtgatttc aatggcgatg gggttggtaa cagatttgga cgcgagcgag aggacgactt
    14641 cgcgttccgc gcccagccat ttggcaaaat acatccccgt aacaatgccc gtaacgctgc
    14701 ccgcaagctg tgaaacgatg acgggcagcc actggttgaa gattttacgg cggttttggt
    14761 agagcggcac ggcaagcacg acgacggcgg gtttcagcca aaaatcaatg aattgcgcgg
    14821 cgttgtggta caccgcataa tcgatgtcga ggattttcag gtaggcaatc agcacgatag
    14881 tgctgacgag tacggggttg cagaagatat tgcccgtgcg cgtgcgcacg ataatcgcaa
    14941 gcgcgtacac ggcaagcgtg aggaaaagca gaacgctggg ctgcctgagg atttcgttca
    15001 tcagataatg ctccgtatcc agcggtgaac cttgcctgta accagcagta cgcacaaagt
    15061 gctggcggag gcggaaacca gtatcgaaaa ccaatcgtcg gcaatcaaat ccaaatagct
    15121 gatgaccgcc acgcagggcg gcacgagaaa cagcgtcaga ttcgccatca gcgcgtcggt
    15181 aagctgttgt agccaagacg ttttgaccca acctgcctgc aaaagcgcaa acagcacgcc
    15241 catgccgacg atgctgccgg gcagtttgat gcctgctagg aaaacggcgg tttcgccggc
    15301 ggcgaggcag ccgaggatga tgaggagcgc gcggatgatg ttcatgttgt gtgtctgtct
    15361 tttgacggcg ttgtgataaa tcttgttaac tgtttaatca taacgtcaaa tatgccgtct
    15421 gaaaagccga agccgcgcac aaacgcaaaa acggcacgga taaaccgtgc cgcagcaacc
    15481 ggggcgtgtt cagactttgc ggtagggatt ttccaaacct tcaatcagcg cgagcaggta
    15541 atcgcgcaac tgacgttcgc cgcaacccat cagcagcgcg tcttcaaacg cgtcttgtgc
    15601 cgtctgatac agctcggcca tattttcgga catcactttg accttttcgg tacaggacac
    15661 gatctgtccg tcatcgtcgt accatttggg catttcgggc atactcattg cgtattcctt
    15721 gggaaatcag ttcagaaagc gggacgggtc gtttttcttg acccggcggg cgaacgcgta
    15781 tttgccgctc caatatttgt ggctcaggct ggtgatttcg atatttttcc ccgtgcgcgg
    15841 cgcgtggatg aagcggttgt tgccgatata aagtccgaca tgggaaatgc ggctgccgcc
    15901 gagcgtgcgg aaaaacacca tatccccggg ctgcaattcg cttcgggcaa ccggcgtacc
    15961 catccgcgcc tgttctgccg acgtgcgcgg caggttgatg cccatggcgc gtttgaagat
    16021 gtgctgcatg aagccgctgc agtcaaaacc ggtagaaatc gatgtgccgc cgtagcggta
    16081 ggcaataccc aaaagtccca tcgcgttgcc gatgagttcg tcggcattgc ccgcccgccg
    16141 ggcgggggct cggttgacgg gtaaaacggg ctgttcgtta agccccatcg cgttgccgat
    16201 gagttcgtcg gcattgcccg cccgccgggc ggggactcgg ttgacgggta aaacgggctg
    16261 ttcgttaagc cccatcgcgc tgccgatgag ttcgtcggca ttgcccgccc gccgggcggg
    16321 ggctcggttg atgggtaaaa cgggctgttc gtcttcggca aactgtctga gaatctgctc
    16381 gcggctgctg agcaggttgg tcaactcgtc ggcaagggcg gggcggacgg caaacatcag
    16441 ccacaaaacc gcccaaactg ccggtttgaa aaaagaatcc atatcggtgt ttccgcgcag
    16501 gaggcatcgt gccgccgtgc gatgttatgc tgtatcagtc gaaaatatct tgttgattgt
    16561 atataaaaaa acggctgttt ggcaaaaaac ggcttgttag attgagttaa tatgttgatt
    16621 tttatataat ttattgataa tttgggaagc gcatccgccg tccgtcttgt tttcgcgccg
    16681 accgcaacca tatagccgcc atccgaacaa tcagtcagag aaaatcatga atcaaaccgc
    16741 catcaaccgc gccgatgtgc gtacccgctt tatcttcgac gatatgcccg tgcgcgggct
    16801 gcacgtccgt ctggaaaacg agtggcagca cattgtaaaa cagaaaaact atcccgccgc
    16861 catccgccgc gccttgggcg agctgttggc tgccggtgtt ctgctgtcgg gcaacctcaa
    16921 aaacgaaggc acgctgattg tgcaggttca ggggcagggg cggctgaaaa tgctggttgc
    16981 ggaagcgact tccgaccgta ccgtccgtgc gaccgcgcgg tgggacgagg cggcagaaat
    17041 cggtgaagat gaaagtttgg tcgatctttt gggcgagggc ggcgtattcg tgctgacgct
    17101 gcagcccaaa gacggcgaac cctggcaggg cgtagtgcct ttggaaggcg acggtatcgc
    17161 gcaaatgttg atgaactata tgaaacgttc cgaacagctt gatacgcaca tcgtcctgtc
    17221 tgcaagcgat gaagcggcgg gcggtctgct ggtgcagcgt ctgcctgaag aggtgttgga
    17281 tgaagaggca tgggaacacg tcagtacgct ggcgcgcacg ctgacggcgg gcgagttggc
    17341 agaattggat gcgcaacatg ttttgtaccg cttgttccac gaaacgccgc cgcgcgtgtt
    17401 cgagccggaa acgtttgaat tttcatgcac ctgttcgcgc ggcaaagtca gcgatatgct
    17461 gttgatgctg ggcggggaag aagtcggcgg cgtggtggtg gaacaaggca gcatcgaagt
    17521 cgattgcgat ttctgccaca gcaaatatgt gtttgacgaa accgatgtca acgcgctgtt
    17581 cggggaggat gtggtcggcg ttgccaaagg gctgccccgg cataccgtcc aataatcttg
    17641 tgcaacaagg ataaatagtc aaatgccgtc tgaagcagct tccgcttcag acggcatttt
    17701 tgcgcgggtt cagacggcat ttaaagcagg aatagggtgg cgagccccaa gaaaatcagg
    17761 aagccgccgg agtcggtaac ggcggtaatc agcaccgagc tgcccagtgc gggatcgcgt
    17821 ccgaactttt ccattaccac gggaattaat acgccgacgg ttgccgccag caggaggttg
    17881 agcgtcatcg cggcaatcat aaccagcccg atgccgaggc tgccgtaaag cagccaagat
    17941 actgcgccca tgaccgttcc ccaaatgatg ccgttgacca aggcgacacc gacttctttt
    18001 ttcaacagcc tgcctgcctg catgcctgtc agttgcccca tcgccatcgc gcggacaatc
    18061 atggtaatcg tctggttgcc cgagttgccg ccgatgccgg cgacgatggg catcagcgcg
    18121 gcgagtgcga cgattttttc gatgctgcct tcaaacgcgc cgatgacgcg gctggcgagg
    18181 aaggcggtgc agaggttgac ggcgagccac atccagcggt ttttgacgga atccaagacg
    18241 ggggcgaaca ggtcttcctc ttcctgcaaa cctgccatgt tcaacatatc cgcttcggat
    18301 tcttcgcgga tcacgtccac catttcgtca acggtcaccc tgccgatgag ctttttgttt
    18361 tcatcgacga cgggcgcggt aaccaagtca tagcgttcga atgcctgcgc tgcttcttcc
    18421 acgtcgtctt cggcgcggaa acgcacgaca tctttcgcca tcacgttttc caccaaatct
    18481 tcgggatcgg cgaccaaaag tttgcggatg ggcagcacgc cttgcagtac gtcgttttca
    18541 tcgaccacaa aaatcttgtc ggtatggtcg ggcaggctgt cgaagcggcg cagatagcgc
    18601 agcaccactt cacaggcgac atcggcgcgg atgctgacca actcgaagtc cataatcgca
    18661 ccgacttggt tgtcttcgta ggacattgcc gctttgactt gtgcgcgctc ttcctcgtcg
    18721 cgcgtctgca aggcttcata aaccacttgg tgcggcaaat cgtctgccag ctccgccaat
    18781 tcgtccgcgt ccaaatcatc gaccgccgcc aacaattcgt ctttgtccat cgactcgatc
    18841 agcgtttcgc gcaccgcatc ggacacttcc agcaagactt cgccgtcgtc ttccggtttg
    18901 accagaatcc agacgatatt gcgttcgcgc ggcggcagcg attccaacac tgccgccacg
    18961 tcggcagggt gcagctcgct caagaggacg gtcagctcgg tcagcttgtc gcgcagcggc
    19021 gcgtcttcga gcggtgtgcc gttttcgatt tgttcaaagg caggttcgag gatttcgcag
    19081 agggagtgga cacggtcgaa atcagcggaa gggcgttcgg catggttttg accggtttcg
    19141 ccgtcgtgtg tcggaggagt gtgttcgatg ctcataaatg ctccgcccgt cgtgtgcggg
    19201 gaggcattcc ggcgggatgg ttattgtcgt ttggaatcgg aaggggcgtt cagtaaaaac
    19261 gaactgggaa ggtccataat aaaagcctga cggtacaggc gcagggttgg aaacggcact
    19321 attctactcc cttttggaac ggtttactat ttttaacgca aatgccgtct gaaacgcggg
    19381 ttccagacgg catttttatc ggcttgttac cagttgtagc ctattttgat tcctgcctgt
    19441 ttgtggcggg cggtgttgct gtctttgccg taagcggcat gaagtttggc gttccagcct
    19501 ttgtatccgg cgtcgatttg cagggtatgc agtgtgccgt gggcggcttg ctgcagtaag
    19561 gtacggtcgt tgacgctgag ggcggaaccg ctgttttggc gggtatggta gtaatcgctg
    19621 ctgaaagcgg gggtcagctt ggcttgaccc agttcgacgg ttttatcgag acggatgccg
    19681 gcatgccatg tggtttggat ttgcgccggg ctgtttatct ctgcgccgtc gagtacgtac
    19741 cggttgccgt tgctgcggtt tatacggatg ccggcatagg gtctgaggtt gatgccggta
    19801 tcgattttga tgccggtgtt gatgcctgca tcccatgcgt ggcggcggac ggcagccccg
    19861 tcataatcgg taaatcgggt acggctgttg ctgtagccta aatctgccgc ggcaaagagt
    19921 gcgccgtttt cccctttgac gaacagatgt gcgccgttgc tgcggtttcg ggcggatacg
    19981 ccttcatcaa aacggttgtt tgtgcgctca tcggttaaaa tcgtaccgac actgagacgg
    20041 tcggtgatgc cggtttggat gccgatatgt gcatagttgg tagtttgttg gtagggacgg
    20101 tgtgtgccgc tatggtagtc ggtttgttgc gtaccggttt ccagccagat gttttgctgc
    20161 tgcggatcgg taaggtggcg gtcgatgcgg cgtcccgcct gttgccggcc ggtattgtag
    20221 gcggcctgtt cggaaacggc ggtgttggcc gaccggctga tcagttcggc caggttcggc
    20281 atacggatgc cggcaacgcc ggttttcaaa ctgtggattt gtcgggacag acggttgagt
    20341 gcgttcaggt aggcgtgccg ggctgtttcg acggcttgcg catcgccgcc ttgccgtgct
    20401 ttgtcaagtt cggattcggc catttctaca cgttctatat acgtatccag ttcggtttcg
    20461 aagagtgtca ggtcgttcgt gtcggcggct ttggcaacct gacggcagat atcgacactg
    20521 tatccttgtg cggcacacag gttttgggca cgcgtcagaa tgccggttag cgggtctgtc
    20581 tgtttcaatt gggcggacag atattggact tggctgtcga tgcgggcaag ttcggtttga
    20641 ctgttctgcc aggcctgtag tgcctgcctg gtcgcgtcag agtcatgttg aacctgtctg
    20701 ctgatgtcgg ttgcctgtaa ttggttgtat gcctgttggt tgcgctcatg ttccgcacgc
    20761 gtggcttcaa tttgaagttc ggcctctttg agcgggttgt acaggctgta tccgttgttg
    20821 tttttgcgga ggatgtagcg gtaggcaccc aaatcggcat agccgttttg gagggtgaat
    20881 cgggcttggt ggctgtgttt cggattgagg ctcacaagtg caagcgattc ggttgtttga
    20941 ggttcttgtc cggtgttttt gacgtggatt tggaatgcgc cgcggctgtc cccttccagt
    21001 ttgagggggg gggcattttg ttttcggacg atgccggtca ggaatcggaa tgtgccgaac
    21061 ccgtcaagtg tgccgttgac ggtcagtgtg ttgaagcggt tgttgtgtgt attattggcg
    21121 aaatcggggt tcagggtaat ttgtgcgccg tcaagcgtca gtgcgccggt gtggctggac
    21181 tgggaaagtg tccagttgct gtctgcttcc atgcggactg cggtgtcttt gccggcacgg
    21241 atgctgccgt acaggtgtgc tttgcccagg cggagctctg aacggtcgtt aagggtaatg
    21301 tcgccgcgta cttgcgttgc aggtagtgca cgatagtttt cggcttttaa aacggcgttg
    21361 ggtgtgcagt gggtgctgcc gctatggtag gagcggtagc attccggtgt tttgccttgg
    21421 gtaaagccga ggtcgatgcc ggacagattg tatgcggtta tgtcgccgtc cagatgcgcg
    21481 gtattgcgcc ctgccgtcag tcgggcatgg tttcgcaggg tgaaccgtgc agccttgaag
    21541 ctgccgtcgg tccattcgtt ttcaagaacg ggttcgcgtt tggcctggtg gtcgtaggca
    21601 tggggtacgg gcctgcctga cacaatcata ttgccgccct caatcaagac ttccccgtta
    21661 aggttcatgc cgccgttgag caacagcgtg ctgtcggtgc gtttgggacg atagtgcagg
    21721 ttgagtgcgc cgttgtaacg gtattcgggc cggccgtata cggcggcatt tgcgatatgg
    21781 cggttttttt ctatggctgc ttcgccggtc atgtccgaga aattcccagt cttgggtgct
    21841 gtaggcattc catgggaagt aggcgccggg gttgccgcct tctttgaggc ggtaatcgtc
    21901 ggtacgcctg cccctgtgcg ggttggcgta ttcgtatagg gcgagggctt gtgtggggtg
    21961 tttggaccat tcacccattc gatattggtt ttgctgtaac tcggtgcggc tttgcccgtt
    22021 tgcgcgttct cacccaagta tccgccgaag gtaattaggt cggggcgggc attttcggct
    22081 tgggcgactt gtttggcggc ctgttgcctg ttgttgccga taaattgcca gcttgttgag
    22141 tttttcatat ttaacgggaa aaattcgcgc gggttgccgc cgggtttgag tatgaagtag
    22201 tcggtccgac ggttgcggtg cgggttgatg tattcgtaaa ccgccgcgtt gccttgcgga
    22261 cggttgcccc attgcaccca ctcgacatgc tcgggactga ggacggggtt gccggtcagc
    22321 gtcagtgtcg cggcttggtc agggttgtgg ttgacgattt gcgcgccgcc gtccgcatgg
    22381 cggatatggg taaaggcaag gttgttgccg ttgaggtcga gccgtccgcc cctgaagccg
    22441 aaatagaggt tttcggattt gatttgctgg ctgtcggcga ggacggccgt gcccctgccg
    22501 ctggtgatgc cgacttggtt gaatgcttgt ttgctgccgt ctgaagcagc tttttgggcg
    22561 agtacgacag tgccttcccc gatgctgatg tcgccctggt tgatgccttg tccgttggcg
    22621 ataagcgtgc ctgcgcccag tttggagagc cggtcgcctt tggggttgct gacttgccag
    22681 aagacgcgtt tgccgtcggc tacgataacg cctgcacctt gccatgtgtg gtttttaccg
    22741 acgacggtga agttgctgtc gaactgcaat gcgcctgcgc cttggttgat attgtctgcc
    22801 agcatcagtg ttttgttgtc gaacttgctg gataggatga gcgttttgcc ggcatcttca
    22861 gacggcatgt gcctgctgtc gttttgtggg gcaagcgaag ggtttgcaat gggcagggtg
    22921 atgcgttcgt tgagcccttg cagggtgctg tcgccattac cgttgtcgcg ccatatgagt
    22981 tcgttggtgg tcagcccgac atccagccgg gtttcgtatt ggcggatggt ggaacggatg
    23041 aattcgggtt gcgtgacgat gtatttgttg aagaaattat cgaagccggc gtaggtgctg
    23101 agtacgcccg caagcaccca gcggttttca tgcttgtcga aggcaaacag gggggaaccg
    23161 ctgtctccgg cgattgcgta ggtgttaagg ggttggtcgg tcaggctgcc gccgacgagt
    23221 aagccgtggt tttggaaccc caatactttc agcggcgtgc cgccggtcag gtattggtat
    23281 gccggggcgg ttcgtgtacg cgtgccgtct gctttgcgga cttgttgcgt gcctgagccg
    23341 agtcgtacaa agtaggggaa gcggtcggta tcgaggtagg cattggcctt tggctggccg
    23401 tttccaagca agggtacgct gctgagtgcg gtaggtgaga tttcggtaac cagtttgttg
    23461 aggcggggaa ggtggtagtc gtagtccggg tgcgggttgc gtgatacgag gcggtaggta
    23521 taggcttgtt cttctggatt ttgggtgtcg ttgccgaatt gtatggaacc gtagccgacg
    23581 ttgtgtttga cactgttgac gtattggggg tgaatcaggg tggcgatggc ggtttgccgg
    23641 ttgctgactc ggaagtcggg catggggatg ccgttgagaa ccctgcctaa tattttgcct
    23701 tgcttgtctt ggatggaaat attgcttgca cctacggcga acgcgccttt attttcggca
    23761 aagtcgcgaa aatattggta atcgacatcg ttgcggacaa tggatgagta agcaggggaa
    23821 acggcaaaaa gaccgagggt ggacaatacg gaacaaaatg gggtggtgtg tgtgaagcgc
    23881 atgatgatta tccgtgtaat agagaaaatc aacgattctt gcggacaaga atcaccgatc
    23941 ccgttttgtg ctggatttat atagtggatt aacaatatag tggattaaca aaaaccagta
    24001 cagcgttgcc tcgccttgcc gtactatctg cggcttcgtc gccttgtcct gatttaaatt
    24061 caatccacta tattatatat tttgtttttg tttgattata aggcaacggt gtcctttcgt
    24121 ctattggcga tgattgtatg attttattta agattttaat aaaaatccta ggaatggctg
    24181 cgttttcccg ttgttttatt tcgtccggta ttggttttta agttcacgca gcgcgacaaa
    24241 ggtatcgagg ctgctgttta acgttttccc gctcaatgcc tcggcgcggt ctctgacgtg
    24301 cggcaggtcg acgcgcaaaa acggattgac gcggcgttcg tgcgcgaggg taacgggcag
    24361 ggtaggcgta tgcgccgccg ctttcagtgc cgtctgaatg tcggcgttgt ccggctcgat
    24421 atgggcggca aaatccaggt tggcggcggt gtattcgtgt gccgggtaga acagggtgtt
    24481 ttcaggcagg cggttgaagc gttggaagct gtcgtaaagc tgttcgattg tgccggtaaa
    24541 cacgcgtccg cagccggcgg aaaacagggt gtcgccgcaa aagacgtgta tgccgtctga
    24601 agtttccata aggtagctga tgtggcggtc ggtatggccc ggggttgccc aaacggtgat
    24661 taacccttcg ccgaaggtaa attgggtgcc tgcggttacg gtgtgggttg ccgcttcgat
    24721 gtcggattcg ccgtaaacgg gcgattccat gtagccgcgc cagagtgccg ccgcaccgcc
    24781 ttcgtggtcg gggtgggggt gagttaccca tgtttgggca agcatgaggc ggttgcggac
    24841 gaggaattcc aagacgggcg agggttcgga agggtcgacg cagacggcat ggttgccgtg
    24901 ctgtatcatc cagatgtagt tgtcggttag ggctttgacg ggggtgattt tcatgagtgt
    24961 tcctcggtgg acggataccg tttaaaggat ggtatgccag aacgccgtct gaaatattct
    25021 tcagacggca ttggtttgag tagggcggtt agcccaaggc tttacgtgcg ccggcaaaga
    25081 ggcggtacca gccggacagt tccgtccagc cttccggttt ccagctcatt tgtgcggcgc
    25141 ggtacacgcg ttcggggtgg ggcatcatga tggtcacgcg gccgtcggcg ttggtaatgc
    25201 cggcgatgcc ttgcggcgag ccgttggggt tgagcggata ggtttgggta acttggtttt
    25261 gaccgtccac atattgcagc gcaatgccca aatcggcgga aatattgccg ccgtgaagcg
    25321 cgaagtcggc gcggccttcg ccgtggctga cgacgacggg caggctggag ccttgcattt
    25381 cgttcagaat cagcgacgcg gatttgggga catgaaccat gctcaggcgc gcttcgaact
    25441 gttcgctcag gttgcgcttg aacttcggcc agcctgccgt gccggggatg atttcggcga
    25501 ggttgctgac catctggcag ccgttgcaca cgcccaatgt cagcgtgttc gggtcggtga
    25561 agaaggcggc gaactggtcg tgtaaggcgg ggtggaacag gatggatttc gcccagcctt
    25621 cgcccgcgcc gagtacgtcg ccgtagctga agccgccgca cgccgccagc attttgaagt
    25681 cggcaaggcg gaaacggcct gccatcaggt cggacatatg cacgtcgtaa gcatcgaatc
    25741 cggcgcgggt gaaggcggcg gccatttcga tttgcccgtt cacgccctgt tcgcgcagga
    25801 tggcgatttt gggtttcgcg ccgctgttga taaacggcgc ggcgatgtct tcgttcacgt
    25861 cgaatttcag gttggcaaac aatgcgctgc ggtcgttgtc gccaatcagg gcaaactcgc
    25921 tgtcggcgca ggcagggttg tcgcgcaggc gttggatttg atggctggtt tcttgccagg
    25981 tttgttgcag tttgattagg ttgtcagaaa taaggtgcgt ttgcccgtcg cggatgatta
    26041 acgtgttctc atcagttaac gtaccgattt caaagacatt atgatgcagc tgttgttgat
    26101 agaataaatt gataatatcg gcaacatctt gttttctaac ttggataaca gcacccaact
    26161 cttcattaaa taatgtgcgg gcaatggttt cttgccattc agccaacgct tttacctctt
    26221 cagtccgcaa tgattgagac agagcggtat ggttggtaat aaatgtttgt gcaagcaata
    26281 aatttaaatc tatatccaag ccgcaccgcc ctgcaaacgc catttctacc aaaacggcaa
    26341 acaagccgcc gtcgctgcgg tcgtggtatg ccaagagttt gtcttcggcg acaagctgtt
    26401 gaatcacgtt gtaaaacgct ttcagacggc ctgtgtcgtc caaatcgggc gcgtcgccgc
    26461 tcatgttgtt gtatacctga ccgaatgcag agccgcccat acgcgctttg ccgaagccca
    26521 aatcgacaaa cagcaatacg ctgtcctcga cgtttttcaa ttcgggcgta acggttttgc
    26581 gtacgtcttg aaccggcgcg aacgcggaga taatcaggct caacggcgaa acgacggatt
    26641 tcttgtccgc gccatcctgc caaacggttt tcatcgacag gctgtctttg cccacgggga
    26701 tgctcaaatc cagtgcctga caggctttag aaaccgcttc gacggtgcgg tagagttttt
    26761 cgtcttcgcc ttcgttgccg cacgccgcca tccagttggc ggagagcttg atgttgccga
    26821 tgtcgccgat gttgaccgcc gcgatgttgg tgatggcttc gccgacgcac attctgcccg
    26881 aagcaggcgc gtcaaacagg gcgacggtcg gtttttcgcc catagacatc gcttcgccgc
    26941 gataggtgtt gaagcccatc atggtaacgg cgcaatcggc tacgggggtt tggtatttgc
    27001 cgaccatttg gtcgcggtgg gtcatgccgc cgacgctgcg gtcgccgatg gtaatcagga
    27061 agtttttggc ggctacggca ggcaggcgca aaacgcggta ggcggcttcg gtgatgtcga
    27121 tatcgtcggc gtgaaacggt tttttagatg gcgtaactgt tttgtcggta cgcgtggttt
    27181 tgggcggttt gccgagcaag acgttcaacg gcaaatcgat ggggttgttg gcgaacaaat
    27241 cgtcgcgcac tttcaaatga ccgtcgtcgg tcgctgtacc gaccacggca aacgggcagc
    27301 gttcgcgttc gcagatggcg cggaaggtat ccaaatcttt ttccaaaatc gacaagacat
    27361 aacgctcttg cgattcgttg caccagattt gcaaaggcgt taaaccgtgt tcttccaacg
    27421 gcacttcgcg cagctcgaat accgcgccgc gtccggcatc gttgaccagt tcggggaagg
    27481 cgttggacaa acctcctgcg cccacgtcgt ggatggagat aatcgggttt ttgtcgccga
    27541 gctgccagca gcggtcgatg acttcttgcg cgcggcgttc gatttcgggg ttgccgcgtt
    27601 gtacggagtt gaagtctaaa gaagcgtcgt tcgtgccggt atccatcgaa gaagccgcgc
    27661 cgccgcccaa gccgataagc atgcccgggc cgcccagttg gatcagcaac gcgccttcgg
    27721 ggatttcgtc tttatgcgtc tgctgcgcct gaatgctacc caagccgccg gcaatcataa
    27781 tcggtttgtg gtagccgcga acctgaccgt caaatttttc ttcaaaagtg cggaagtagc
    27841 ccaagaggtt cgggcggccg aattcgttgt tgaacgccgc gccgccgatg gggccttcga
    27901 tcatgatgtc cagcggcgag gaaatatgtt ccggcttgcc gtagtcttgt tcccacggct
    27961 gtttgaggtc ggggatgttg aggttggaca cggtaaagcc ggtcaagcct gctttcggac
    28021 gcgaaccttt gcccgttgcg ccttcgtcgc ggatttcgcc gcccgcgccc gttgccgcgc
    28081 ccgcaaacgg cgcgatggcg gtcgggtggt tgtgcgtttc cactttcatg atgatatgcg
    28141 tgtcttcctc gtggaaacgg tagccttggt tttccgccgc attcggatag aaacgctcga
    28201 ttttcgcgcc ttcaatcacg gacgaattgt ctttataggc gacgaccgta ccttcgggat
    28261 gcgcgttgtg cgtgtcgcgt atcataccga acagggattt cggctgtttt tcgccgttga
    28321 ggatgaagtc ggcgttgaag attttgtggc ggcagtgttc gctgtttgcc tgcgcgaaca
    28381 tcatcaattc aacatcggac ggattgcgct gcaaagcctg atagttttca accagataat
    28441 cgatttcgtc ggcggagagt gccaagccca tttcggtatt ggctttgacc aaagcctctt
    28501 taccgccgcc caaaacatca acggtggaaa aggtttcgga ttcgagatgg tggaataatt
    28561 tggatgccgt ctgaaaatcg ggcagcacgg attcggtcat gcggtcgtgc agcaacgccg
    28621 cccattgctg tttctcatcg tcgttcagac gaccttccag ccacaccgcc atgccgcgct
    28681 cgatgcgttc gatgcctgcc aaaccgcagt tttccgcgat attggtcgcc ttggaagccc
    28741 acggcgaaat cgtacccaaa cggggcgtta ccaaaaacaa atgcaagccc tcgcgcgctt
    28801 ttggcgtttg ttcaacgctt tgcgccgcca acaaggcttg cagtttttcg acagtcgcgg
    28861 catccaatgc tttctcgctg ccggcgaaat accaaaattc gctgcttaat ttgacttcgg
    28921 gcagtccgag tgcggcggct ttttgcaaga gtttttcgac acggaaatcg gaaagggcgg
    28981 taacgccgcg caagggcaaa acgacagaca tggattcggc tctcaaatgc ggttggaaaa
    29041 tctgtcatta tacgcgcaac ggggctgtat tgcttgggta ttttggctta aaatgttgac
    29101 aatcttcaga cggcatacag ttggggaaaa tacaggtgcc gtctgaagat attaaggaat
    29161 aagggtttta tctgtttttc tgatttttac ccaaagccgg gtatgcggat aattgcagga
    29221 agtagagggg cgggcaactg cttccgtatg tttttagatc aaatgccgtc gtttccgtac
    29281 ggttaggaat atggaagttt ggatttacag aaacctttaa atattgctga aatgcagaga
    29341 tcgggattcc cgcctgcgcg ggaatgacgg tttccggcaa atcgccgcat cgccgggcga
    29401 acagtaccgt ttatcgtttg ccggcggttt ctgctatatt tgtcgtttgt ttacgttaat
    29461 taatggagaa tgtgatgaaa aaaatcgagg cgattgtcaa accgttcaaa ctcgacgacg
    29521 tgcgcgaggc gttgacggaa atcggcatta cggggatgac cgtcagcgag gtcaaagggt
    29581 tcggcaggca gaaggggcat acggaaatct atcgcggtgc ggaatacgcc gtcgatttcc
    29641 tgcccaaggt caaaatcgag ctggtgttgg cggatgacgc tgtggaacgc gcgattgacg
    29701 tgattgtcga ggtggcgtgt tcgggcaaaa tcggcgacgg caagattttt gtgctgccgg
    29761 tcgaggaggc aatccgtatc cgcacgggcg aacgtgcgga cgcagcagtc tgacggccgg
    29821 atagaaaata aaaaccgcag ccttgcaggg ctgcggtttc tttatcggga gatgctgctt
    29881 accactcgta attgacgccg acatggtagg ctgcggaaga accggacgaa gtgccgactg
    29941 ccacgcctgc tttggcggca aagttttcgg taaagcggaa gccggtaccg atggcgactg
    30001 ccgattcgga tttgtagccg ccgactgcag ccgttacatt gaaccgaccc acgttgtaag
    30061 gttggaacag accggagagc gcggcttgtt ctgcaaggcc ttggcgggtt tctttgcgca
    30121 ggtctgacac tgttttatcc aaaccgttca ggcgagtatc gtgatcggca atggattttt
    30181 cagcagaagc caagcgtgtg tccaattttt cggtagtagc gttcagacca tcaattctga
    30241 caaatttgct gtcagactct tctctggtgt acacgtcggc actgtttgct tttttagcaa
    30301 tattatcttt gttcgtagcg atatcagctt tgatgtcggt aacttttgca gcgacagctt
    30361 cggccttgtc ggctgcagta ttagctgtgc cagcggcagc ttcggctttg cctgctgcag
    30421 tttctgcagc ttttactttg gcatcgacgt tttgtttgtt ttcattgacg gttttggtca
    30481 ggttagtcac gactttttca gacccagacc tttaaagtcg tcggcttcaa catcggctgc
    30541 agttgcgtct tttttggtaa ttgtgccgtc ttcaccaatg tcgtagatgg tctctccagc
    30601 tttgaaaccg ttgatttctt ggccattgtt gtaggcagca gcaatggcca cagtggcagc
    30661 ttttttaaca tcgtcgtcgt ttgtggctgc cagtgcgccg ctacagaaag tggcaaggat
    30721 ggctgcggtc agtactttgg attggaaatg tttcatggct cattaccttt gtgagtggtt
    30781 aaaaaatggg gcgaaaatct tttgcaagta ttttatttcc accaagtttg tttatatatt
    30841 gaagtatata tgaagggagg catatataca atacattgtc gcaatttatt tatttattta
    30901 tttatttgat tcatgcaggt aggtagttcg gcattaatat ctgttaatat gtgcatatta
    30961 tgctgatgtc aattctgccg gtgtcgtgtc ttaattttgt caggatatgg gttgccgtct
    31021 gaatgttcag acggcctttt tgtcggattt agatatggtg gacaatcagt tttttgcctt
    31081 cgtagtcgag tacgtccacg tccatgtcga acagttcttt gatgttgtgt acggtgaaca
    31141 cttcttcggg cgtgccggtc aggacggctt tgccgttttt catggcgacg acgtggtcgg
    31201 cgtaagcggc ggcctggtta atgtcgtgca atacggcgac ggtggtgcgt ttgtgttcgc
    31261 gcgtgagctt ttgcagaatc tgcatgagcg agcgggcgtg atacatatcg aggttgttca
    31321 gcggttcgtc caaaaggacg tagtcggtgc tttggcagaa caccatcgca atcatggcgc
    31381 gttggcgttg tccgccggag agttcggtca gatagcggtc ggagaggtct tgcaggtgga
    31441 attcttcgat tgcaccgtta acgatacggc ggcattcggc agtcggcctg ccttggtggt
    31501 agggatagcg gccgaacatc agcaggtcgc gcacggtgat gcggctcatg atgctgtttt
    31561 cttgggtgag gatggagagc gtgcgggcga gttcggcggt gggagtgccg gcaaggtttt
    31621 tgcctcggta ggcgatgctg ccgctttcaa gcgggcgcag ccgcgccata aaggaaaaca
    31681 gggtggactt gcctgcgccg ttcgggccga tcagcgcggt aatgccgcct tcgggaatgt
    31741 cgaggctgat gttgtccaga atggggcgcg tgccgatgcg gtagctgacg ttgcggatgg
    31801 taatcatagg cggatgcttg tcgggcgtag gtttgttgtt gtttggcggg gttcagacgg
    31861 catttgaaat tcggaatgcc gtctgaacgc tcacacttcg cggacgatga cgaactgcgg
    31921 gtcgtccgcc gattcggtca aaaatgcttt aacgcgtgcc tgccagagtt gtccgaagcg
    31981 ttcgagttct tctgcgcttg cgctgccgcc gacggctttg ggcataagtt cgcgcatttc
    32041 gggtgcgaag ggtttcaggg cggcgttgat gctgacggcg acggttttac cgttgtcttt
    32101 gcggcgcagg gtcagtgtgc cgttgatttc gcctgcacca aaggataaga ggttgcggcg
    32161 ggcgaagcgt ccctgtattc cgatgcctcc gaagccggtt tcgggggctg cgccggtaag
    32221 gagttggacg acggacgcgg ttacgccgac cgtgccttcg tcgcgcgctc cctgcatggc
    32281 ggcttcgatg ccgccgcgtt cgggcagctc ttcgccgtaa agggctttca gtcctttgat
    32341 aaccatcagg tacgcgcccg cgacggtcgg gcaggaatgt ccgcacaggc gcacggcatc
    32401 ggcgtagcgg taagtgagga tgccgtcttg cgccgcgccg aggaatgcgg caagcgggtc
    32461 ttgtacggtc agcgtcgggg cgcggtcgaa aaattcgggt aaacgttctt gcgtcatatg
    32521 gtttgtcctt tcggggcggg ctgccgttca gacggcatcc gtcatttttt gtgttttaaa
    32581 acgagataga ggaaaacgag tccgcccgca aattcgacca ccacgcttaa taccgccttc
    32641 atgcccaaga agtgttcgaa tacggtctgt ccgccgacca agaggatgcc gccgacacaa
    32701 accgtcatcg gcaggcggac ggaatggcgc acggacgggg aaaagtggtt ggcaagcgag
    32761 gcggcgagaa gcccgaaaaa gcttaccggg ccgacaacgg cggtcgccgt cgccaccagc
    32821 gcggcaatcc aaagcagtat ccataaggtg ttgcgcgtgt agctgatgcc caaattgacg
    32881 gcttggtcgc gccccaaaag gtgtacgtcc aagcggtagc gttcacgcca aacgaccgcc
    32941 gcgctgacga gcagaatcag cgcgcctatg cccaaaagct cgctgtggac ggtattgaat
    33001 ccggcaaaca tattcgcctg cgccgccgta aattcttcgg ggtctatcat gcgcgaaagc
    33061 agcgaggaaa ggctgcggaa caaaatcccg aaaatcacgc cgattaaaat catacgcggc
    33121 aaatcgcgcc cgccctgacg gatgagcgtg tagaacaaca gcagcgagcc gcccatcata
    33181 acgaccagtt caaagccgaa tttgcccgtc aacggcaggg aagcatagcc cacgccgccc
    33241 agagcgaaaa ccaaaagcgt ctgcaaaaac acatacagcg aatcgaaacc caaaatcgaa
    33301 ggggtcagaa tcggattgtt ggtcagcgtt tggaagagtt gcgtggacac gccgaccgca
    33361 taggcgacca tcagcagcgc ggcaagtttg gtcagccgca ggtgcagtac aaaatcccaa
    33421 tcgcctttga cgttgagcgt cataaacagt ccgcaggaaa ccagcaacag cgcaaaggcg
    33481 acccacaacg gacggctgct tcctgccata aaaccgatat ttttttcaga cggcataggc
    33541 gggtttcctc aacaaaagcc acaaaaacaa cgccgtaccc aatacgccga agacggtcga
    33601 taccggaatt tcaaacggaa acacaatcac gcgtccgata atgtcgcaca ataaaaccaa
    33661 agacgcaccc agcaaagcca ccgcaggcag gctttggcgc agcctgtcgc ctatcaggcg
    33721 gctgatgatg ttcggcacga ccagcccgat aaacggaata ttgccgaccg taacgataac
    33781 cagcgacgta atcaaagcca caataatcag ccccgaccac agaatcgccg tccggttcag
    33841 ccccaagttc acgcttaccg tttcgcccaa acccaaaatc gtcagccggt cggcaatcag
    33901 ataggcaaac accgccaaac cgcccgtaat ccaaagcagc tcgtaccgcc ccagcagcac
    33961 gctcgaaaaa tcgccctgct gccatacgcc caacatttgc agcatctcaa actcatacgc
    34021 gacaaacgtc gccaccgcct caaccacacc gccgaaaata atccccacca gcggcaccat
    34081 cagctgcgcc gtcggcggca ggcggcggat aagcagcata aacaccagca tcccgattaa
    34141 cgcggcaacg gcggcaaccg acattttgac cggcagcggc gcggcaggca gcagcaggga
    34201 catcagaagc aaacccaaag ccgcactctg acccgcgccc gccatagaag gctcgacaaa
    34261 acggttacgc atcaaaatct gcataatcat ccccgccacc gccatcgacg cgcccgtcag
    34321 cacaatcgca aacgtgcgcg gcaggcggct gatgaacata acctgctggc tgtcggacag
    34381 cgaaaacaca tccgaccagc ggaaatcggc aacgcccacc gacaggctga cggcaaacaa
    34441 caccgccagc agcaggaggt tggtcaggtt gagggaaaaa ggtttggcag tcataaacag
    34501 aagggaaaag cgttaaggcg tagaagattc aaacaaggca gtccgaaccg tcggagcgga
    34561 aagccttgtt tgaagcctcc ggatgggcag aggatagggc aagttccaat aaaaacgctt
    34621 gccgccattc ccgc
//
LOCUS       DAXHTY010000009        34462 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXHTY010000009 DAXHTY010000000
VERSION     DAXHTY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.009"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..77)
                     /locus_tag="ACFNMK_000378"
     CDS             complement(<1..77)
                     /locus_tag="ACFNMK_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6363713.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTART"
     gene            complement(383..1642)
                     /gene="rho"
                     /locus_tag="ACFNMK_000379"
     CDS             complement(383..1642)
                     /gene="rho"
                     /locus_tag="ACFNMK_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC6363714.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(1862..4246)
                     /gene="ppsA"
                     /locus_tag="ACFNMK_000380"
     CDS             complement(1862..4246)
                     /gene="ppsA"
                     /locus_tag="ACFNMK_000380"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC6363715.1"
                     /translation="MADNYVIWFENLHMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGNRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4657..5478
                     /locus_tag="ACFNMK_000381"
     CDS             4657..5478
                     /locus_tag="ACFNMK_000381"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC6363716.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5717..6379)
                     /locus_tag="ACFNMK_000382"
     CDS             complement(5717..6379)
                     /locus_tag="ACFNMK_000382"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6363717.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPPKSMDEIRNQ
                     ASHGAAGLLKLGAGITPDHPDYARWRTEYLDEYDSRYAQDTELFDGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLAPKLGFIIPPAVVVSGDTCGEPKPSVKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QS"
     gene            complement(6392..7219)
                     /locus_tag="ACFNMK_000383"
     CDS             complement(6392..7219)
                     /locus_tag="ACFNMK_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC6363718.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAETNAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPVALFRSLITRYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
     gene            7498..8121
                     /gene="lolA"
                     /locus_tag="ACFNMK_000384"
     CDS             7498..8121
                     /gene="lolA"
                     /locus_tag="ACFNMK_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC6363719.1"
                     /translation="MMKPHNLFQFLAVCSLIVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            8394..9533
                     /locus_tag="ACFNMK_000385"
     CDS             8394..9533
                     /locus_tag="ACFNMK_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6363720.1"
                     /translation="MKKTLVAAAILSLALTACGGGNDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            9666..9893
                     /locus_tag="ACFNMK_000386"
     CDS             9666..9893
                     /locus_tag="ACFNMK_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6363721.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKTTNNKQHPIPNTKHQIYISG"
     gene            9901..10659
                     /locus_tag="ACFNMK_000387"
     CDS             9901..10659
                     /locus_tag="ACFNMK_000387"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6363722.1"
                     /translation="MSGARNTGIKNSNGKYIVFIDSDDFINCQVLLDFLSKDDIDMPD
                     VVFLNAVKYDKGSVSHFGEDYQPEKILNQSKVEVLKGLCRFRKFPGSACNKIIKRELI
                     IKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQGRKDSKTGTVSEKSIKS
                     LLYILEKNAEMEFDRDISSYLYSFLSYEYLVLLFIMTSKNIECDADIKRRAYHLRFML
                     LKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            10700..11230
                     /locus_tag="ACFNMK_000388"
     CDS             10700..11230
                     /locus_tag="ACFNMK_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC6363723.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYIGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(11264..11380)
                     /locus_tag="ACFNMK_000389"
     CDS             complement(11264..11380)
                     /locus_tag="ACFNMK_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HFC6363724.1"
                     /translation="MPSKPEKRASDGIFACNSNADGQNQGASDTVIGCIPSA"
     gene            complement(11605..13200)
                     /locus_tag="ACFNMK_000390"
     CDS             complement(11605..13200)
                     /locus_tag="ACFNMK_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC6363725.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGRFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(13307..13702)
                     /gene="hisI"
                     /locus_tag="ACFNMK_000391"
     CDS             complement(13307..13702)
                     /gene="hisI"
                     /locus_tag="ACFNMK_000391"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC6363726.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(13733..14500)
                     /gene="hisF"
                     /locus_tag="ACFNMK_000392"
     CDS             complement(13733..14500)
                     /gene="hisF"
                     /locus_tag="ACFNMK_000392"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC6363727.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPGNTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(14513..15250)
                     /gene="hisA"
                     /locus_tag="ACFNMK_000393"
     CDS             complement(14513..15250)
                     /gene="hisA"
                     /locus_tag="ACFNMK_000393"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC6363728.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPADTALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPSRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(15283..15921)
                     /gene="hisH"
                     /locus_tag="ACFNMK_000394"
     CDS             complement(15283..15921)
                     /gene="hisH"
                     /locus_tag="ACFNMK_000394"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC6363729.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCIAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDRQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FDPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(16052..17554)
                     /gene="pta"
                     /locus_tag="ACFNMK_000395"
     CDS             complement(16052..17554)
                     /gene="pta"
                     /locus_tag="ACFNMK_000395"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC6363730.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDIVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(17739..18797)
                     /gene="fbpC"
                     /locus_tag="ACFNMK_000396"
     CDS             complement(17739..18797)
                     /gene="fbpC"
                     /locus_tag="ACFNMK_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC6363731.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(18818..20335)
                     /gene="fbpB"
                     /locus_tag="ACFNMK_000397"
     CDS             complement(18818..20335)
                     /gene="fbpB"
                     /locus_tag="ACFNMK_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC6363732.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(20403..21398)
                     /gene="fbpA"
                     /locus_tag="ACFNMK_000398"
     CDS             complement(20403..21398)
                     /gene="fbpA"
                     /locus_tag="ACFNMK_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC6363733.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(21649..22119)
                     /locus_tag="ACFNMK_000399"
     CDS             complement(21649..22119)
                     /locus_tag="ACFNMK_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HFC6363734.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAIHSAMLIHRAADALRKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(22176..23552)
                     /gene="argH"
                     /locus_tag="ACFNMK_000400"
     CDS             complement(22176..23552)
                     /gene="argH"
                     /locus_tag="ACFNMK_000400"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC6363735.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLIMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMSA"
     gene            complement(23571..24440)
                     /gene="galU"
                     /locus_tag="ACFNMK_000401"
     CDS             complement(23571..24440)
                     /gene="galU"
                     /locus_tag="ACFNMK_000401"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC6363736.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYERSGNSILGA
                     ETVEPSQTGSYGIVETEHLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEAIVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(24468..25067)
                     /gene="rdgB"
                     /locus_tag="ACFNMK_000402"
     CDS             complement(24468..25067)
                     /gene="rdgB"
                     /locus_tag="ACFNMK_000402"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC6363737.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSTFGIPECP
                     EPYSTFVENALAKARHAAKYSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALADLIHKLAL"
     gene            complement(25060..25293)
                     /locus_tag="ACFNMK_000403"
     CDS             complement(25060..25293)
                     /locus_tag="ACFNMK_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC6363738.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAQSAAKENRYV"
     gene            complement(25401..25934)
                     /locus_tag="ACFNMK_000404"
     CDS             complement(25401..25934)
                     /locus_tag="ACFNMK_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC6363739.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(26035..26493)
                     /gene="nudB"
                     /locus_tag="ACFNMK_000405"
     CDS             complement(26035..26493)
                     /gene="nudB"
                     /locus_tag="ACFNMK_000405"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC6363740.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFDMEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            26703..26779
                     /locus_tag="ACFNMK_000406"
     tRNA            26703..26779
                     /locus_tag="ACFNMK_000406"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26737..26739,aa:Pro,seq:ggg)
     gene            26937..28763
                     /gene="mafB"
                     /locus_tag="ACFNMK_000407"
     CDS             26937..28763
                     /gene="mafB"
                     /locus_tag="ACFNMK_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC6363741.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VSDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            28766..29230
                     /locus_tag="ACFNMK_000408"
     CDS             28766..29230
                     /locus_tag="ACFNMK_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363742.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            29455..29601
                     /locus_tag="ACFNMK_000409"
     CDS             29455..29601
                     /locus_tag="ACFNMK_000409"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363743.1"
                     /translation="MWYEANIGLINTMSRSNQAGTRLLYSNYGLLYITTDHYISATRF
                     VAWK"
     gene            29613..29900
                     /locus_tag="ACFNMK_000410"
     CDS             29613..29900
                     /locus_tag="ACFNMK_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFC6363744.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            30414..31652
                     /locus_tag="ACFNMK_000411"
     CDS             30414..31652
                     /locus_tag="ACFNMK_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HFC6363745.1"
                     /translation="MIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEALKKDKQ
                     TARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKESLYYLN
                     KHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGSCAETFG
                     LGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEYVQFSID
                     LFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAH
                     AQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVE
                     IKKPDGSWEMKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSK
                     SGIKIEGFTEPNRTAYPIYE"
     gene            31683..32102
                     /locus_tag="ACFNMK_000412"
     CDS             31683..32102
                     /locus_tag="ACFNMK_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363746.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            32139..33113
                     /gene="mafB"
                     /locus_tag="ACFNMK_000413"
                     /pseudo
     CDS             32139..33113
                     /gene="mafB"
                     /locus_tag="ACFNMK_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            33124..33585
                     /locus_tag="ACFNMK_000414"
     CDS             33124..33585
                     /locus_tag="ACFNMK_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363747.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            33572..33859
                     /locus_tag="ACFNMK_000415"
                     /pseudo
     CDS             33572..33859
                     /locus_tag="ACFNMK_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
     gene            33886..34305
                     /gene="mafI"
                     /locus_tag="ACFNMK_000416"
     CDS             33886..34305
                     /gene="mafI"
                     /locus_tag="ACFNMK_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MafI"
                     /protein_id="HFC6363748.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            34395..34462
                     /locus_tag="ACFNMK_000417"
                     /pseudo
     CDS             34395..34462
                     /locus_tag="ACFNMK_000417"
                     /inference="COORDINATES: protein motif:HMM:NF026862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
BASE COUNT         8248 a         7991 c         9679 g         8544 t
ORIGIN      
        1 gttcttgcag ttacacctgc gacaaacagt tcaatgagtt tattttgttt ataccggctt
       61 agacgacttt ttctcataaa ggcaactcta acttaatttg aatttcccta gttatctagg
      121 acagccccaa atttatacaa aaatgagcgc ggttcggcgc aaccttgaat caagttcccg
      181 catcggtttt cattgccggt acggatgcgt tcaagccggc tttgcaaagg ccgcgctttc
      241 ggcaagcgga cacggacact gccgacggtt gcgccgttaa cggggggagg gaggagctgc
      301 gccgaccgtg tgaatgaaag tgccgtctga aacccgattt tcaggcttca gacggcattt
      361 cgcattaatg cgggcggcgc gtttatttgc cgcgcatcag ctcgaagaaa tcgtcgttgt
      421 ttttagaggc tttgattttt ccgattaaaa attcggctgc ctcgatttca tccatcgggt
      481 gcaggaactt gcgtaagagc cacatacgtt gcaactggtc gttcggcaca agcagctctt
      541 cgcggcgcgt gccggatttg ttgatgttga tggcggggaa gaggcgtttt tccgccatac
      601 ggcggtcgag gtgcaattcc atattgccgg tgcctttgaa ttcttcgtaa atcacatcgt
      661 ccatacggct gccggtttca accaatgctg tggcaatgat ggtcagcgaa ccgccttctt
      721 ccacgttgcg cgccgcgccg aagaaacgtt tgggacggtg cagtgcgttg gcatcgacac
      781 cgccggttag gattttgccc gaggtaggca cgacggtatt gtaggcgcgg gcaaggcggg
      841 taatcgaatc aagcaggatg accacgtctt ttttgtgttc caccatacgc ttggcttttt
      901 caagtaccat ttcggcaact tggacgtggc gttgcgccgg ctcgtcaaat gttgaagaaa
      961 ccacttcacc tcggacggaa cggctcattt cggttacttc ttcgggacgc tcgtcaatca
     1021 agaggacgat gagttcgact tcgggatagt ttgcggtaac ggcgtgggca atgttttgca
     1081 gcatcacggt tttaccgctt ttgggcgggg caaccaagag ggcgcgctga cctttgccga
     1141 tgggggaaat caggtcgatg gcgcgtccgg tcaggttttc ttcggacttt aagtcgcgtt
     1201 ccagcttcaa ctgttcggtc ggaaacagcg gggtcaggtt ttcaaacagg attttatggc
     1261 ggcatacttc cgggtggtcg ccgttgatgg tatcaagcct gaccagggca aaatagcgtt
     1321 cgttgtcttt tgggacgcgc acgcttcctt cgatggtgtc gcccgtatgc aggttgaagc
     1381 ggcggatttg ggtgggcgag acatagatgt cgtcggggcc ggcaagatag gacgtgtccg
     1441 cgctgcggag gaagccgaag ccgtcgggca ggatttcaag cgtgccggag caggtgaaac
     1501 cctcgccttt tttcatcatc tggcggacga tggcaaatac gaggtcttgt ttgcggaatc
     1561 ggttggcgtt ttcgatgccg tgttcttccg ccaattctaa gagtttggaa atgtgcaggg
     1621 tttgtaattc ggagacgtgc ataataatga tgtattttga agaggaaaaa gacaggcaga
     1681 tgccgtctga aagaagaagc tgaccgttgc cggttgctcg gggaaggggg aattgtaggc
     1741 agtcggcgcg tgggtgtcaa atattatcgc ggacggggca tcggcaggaa atgccgtctg
     1801 agcggagctg cttggaaaaa aatacccccg cgcttttcag gctcgggggt atgggcattg
     1861 attatttgtt caattcattc gccaaatata gccaagtttc gatgacggta tccgggttca
     1921 gggaaacgct ttcgatacct tcctcaacca gccatttggc gaagtccgga tggtcggacg
     1981 ggccttgacc gcagatgccg acatatttgt tctgcttgcg gcaggcggag atggcaaggt
     2041 gcagcatcac tttgacggca gggttgcgtt cgtcaaacga ttcggatacc aagccgctgt
     2101 cgcggtcgag accgagggtc agttgggtca tgtcgttcga gccgatggag aagccgtcga
     2161 agtattgcag gaattgttcc gccaataccg cgttgctcgg cagttcgcac atcataatca
     2221 ggcgcaggcc gtttttgccg cgttccaagc cgttttcttt cagggctttg acaacggctt
     2281 cggcctcgcc caaggtgcgt acgaacggaa tcatgatttc aacgttggtc agccccattt
     2341 cgtcgcggac gcgtttcaag gctttgcatt cgagggcgaa gcagtctttg aaattgtcgg
     2401 cgacataacg cgctgcgccg cggaagccca gcatcgggtt ttcttcgtgc ggttcgtata
     2461 cgctgccgcc gaccaggttg gcgtattcgt tggatttgaa gtcggacata cggacgatgg
     2521 ttttacgcgg ataaaccgat gcggccaatg tcgccacgcc ttcggcgatt ttatcgacgt
     2581 agaagtcgac aggggacgcg taaccggcga tacggcgggt aatttccgct tttaattcgt
     2641 catcttgttt gtcaaattcc aacaaggctt tcgggtggat accgatttgg cggttgatga
     2701 taaattccat acgcgccaag ccgatgcctt cgctgggcag gttggcgaag ctgaatgcga
     2761 gttcggggtt gccgacgttc atcatgactt tgacgggtgc tttgggcata ttgtccaagg
     2821 cgacatcggt gatctgtacg tccaacaggc cggcatagat gaagccggta tcgccttcgg
     2881 cacaggatac ggtaacttct tgaccgtttt tcagcaattc ggttgcattg ccgcagccga
     2941 caacggcagg aatacccagt tcgcgcgcga tgatggcggc gtggcaggta cgtccgcccc
     3001 tgttggtcac gatggcggaa gcgcgtttca tcaccggttc ccaatccggg tcggtcatat
     3061 cggtaacgag tacgtcgccg gcttcgacgg aatccatctc ggaagcatct ttaatcaggc
     3121 gtaccttgcc ctgtccgact ttctgaccga tggcgcggcc ttcgcataat acggttttgt
     3181 cgccgttgat ggcgaagcgg cgcaggttgc ggttgccctc ttcttgggat tttacggttt
     3241 cgggacgggc ttgcaggatg tagagtttgc cgtccaagcc gtcgcgtccc cattcgatat
     3301 ccatcgggcg gccgtagtgt ttttcgatgg tcagtgcgta atgcgccaac tcggtgattt
     3361 cttcgtcggt aatggagaag cggttgcggt cttcttcggg gacatcgacg ttggttaccg
     3421 atttgccggc ttcggctttg tcggtaaaaa tcattttgat gtgtttcgaa cccatggttt
     3481 tacgcaggat ggcgggcttg cccgctttga gcgtgggttt gaacacataa aattcgtccg
     3541 ggttgaccgc accttgtacg acgttttcgc ccagaccgta agaggaggta acaaagacga
     3601 cttgatcgta gccggattcg gtgtcgaggg tgaacatcac acctgatgcg ccgctgtcgg
     3661 aacgcaccat gcgttgaacg ccggcggaaa gggcgacgat gtcgtgttcg aagcctttgt
     3721 ggacgcggta ggaaatggcg cggtcgttgt acagggaggc gaatacatgg tgcatcgctt
     3781 ctttaacgtt ttccaatccg ttgatattca agaaggtttc ctgttggccg gcaaatgatg
     3841 cgtcgggcag gtcttcggcg gttgcggaag agcgtacggc gacggaaatg tccgcgcctc
     3901 cggcatcggc gaccattttg ttccatgccg cttcgatttc ggcatcgagc tgttcgggga
     3961 aaggcgtgtc caaaatccat tggcggattt ctttgccgac gcgtgccagt tcggcaacgt
     4021 cttcgacatc taattttgcc agtgcggcgg aaatgcgttc gctcagaccg ttgtgtgcga
     4081 ggaatgcgcg gtaggcatcg gctgtggtgg caaagccgcc ggggacgcga acgccttttt
     4141 cggtcagctg actgatcatt tcgcccagcg aggcgttttt accgcccacg cgttcaacat
     4201 ctgtcatatg caggttttca aaccagatta cgtagttgtc ggccatttgt gtgtccaatc
     4261 caaaatatgt taaaaaagaa acaaatccgc gtgcttattt taagcgattc gttcctctgc
     4321 tgtcatgtgt tttatccgtt ttaaaatcat gatgccgtct gaaaaattgc ggtttcggcg
     4381 tgtgtagcgg tttgaaactt gcagccggta tacttctttt tttgggtatt ttctttgtaa
     4441 aacaggtggt ttgaataggt taatgttttt tctgtttgat ttttttgttt attttttaaa
     4501 attttctgcc aaaaaatact ttatataaat aattttattt caaaattata ttgtgtctgt
     4561 ttgggtgtaa tccgaggtag gcgtgctgcg ggtgctttcc ttgtgtctgc cgctgctgtt
     4621 atgatgatat ttttattctg tatttaagga tggaagatga gcagtccgcg ccatgtgttt
     4681 tacatttccg accgtaccgg tctgactgcc gagaatatcg gcgaggcttt gctgaaccag
     4741 tttggcaatc tgtcgttcaa acgccatacg catccgtttg tcgatacgcc ggaaaaggcg
     4801 cgcgcggtgg tggagaaggt caatcggagc cggcaggaaa acggtcagcg tccgattgcg
     4861 tttgtcagtg tggttgatga cgaaatccgt cggattatca aaggggcgga tgcttttcag
     4921 attaatttct ttgagacttt tttgggactg ttggagaaag aactcaatac cgaagccacg
     4981 gcatccgagc aggggcatca cagtatcggt aatacgaagc gttacgatgc gcgtatggag
     5041 gcggtaaatt tctcattgaa ccacgatgac ggggtcagcg ataagaacct tcaggaagcg
     5101 gatgtaatct tgatgggtgt atcgcgttcg ggcaaaacgc cgacctgcct ttacctcgcc
     5161 ctgcaatacg gcatccgtgc ggcaaactat ccgctgattc ccgacgattt ggaatcggcc
     5221 gatctgccgc gtatggtcaa gccttatagg gataagctgt tcgggttgac catccagccg
     5281 gaacgtttgc aggccatccg ccaagagcgc cgcccgaatt caacttatgc caaaatcgat
     5341 acatgccgca gcgaggtggc ggacgcgcag agtatgttca gacggcatgg cattccgttt
     5401 gcgaatacga cggataaatc agtggaagag cttgccgtac acatccttca ggcgtgcaag
     5461 ctcaaacgca ggttttgacg ggctttgatt cggtttgaag gcggaaatgc cgtctgaaat
     5521 caggtttcag acggcagttt tatagtggat taacaaaaat caggacaagg cgacgaagcc
     5581 gcagacagta caaatagtac ggagccgatt cacttggtgc ttcagcacct tagagaatcg
     5641 ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca ctatatgttt
     5701 gtggggcgga tatttttcag gactgtattt tgtccagaca ttcgagcaga tcgagcggcg
     5761 tgcggatgtg gaaatccgcc tgccatgagc cggtatcgtc ttcgggagcg atgtagcccc
     5821 attcggcgag gacggttttc atgccggcgt tgcggcctgc ttggatgtcg cgttcggcat
     5881 caccgacgta gagtgtgtgt tgcgggtcgg cgtggatttg tccgcacgca tacagcatgg
     5941 gtttgacgct gggcttgggt tcgccgcagg tgtcgccgct gacgacgacg gcgggtggga
     6001 tgatgaagcc gagtttgggg gcgagtttgt cggtgaagcg catgggtttg ttggtgatga
     6061 tgccccattt gatgccgcgt ctgtcgagtt cggcgatgag ttcgtttacg ccgtcaaaga
     6121 gctcggtgtc ttgggcgtag cggctgtcgt attcgtcaag gtattcggtg cgccatcggg
     6181 catagtcggg gtggtcgggg gtgatgcctg cgccgagttt gagcagtcct gccgcgccgt
     6241 ggctggcttg gttgcggatt tcgtccatgc ttttgggcgg cagtccgtgg cgggcgagca
     6301 gggtattgag tgcgccgccg aggtctaggg cggtgtcggc aagcgtgccg tcgaggtcga
     6361 agagtacggc gtgtatcatg tgtgttcctt ttcagacggt atttttgttc tgtttcaaac
     6421 cctgctcgaa atcttccaac atatccaatt caaagcggct gaagcccgct ttttcccgcg
     6481 cttcgatgtt cacatagcca cggaagataa acatatcgta acgggtaatc agactgcgga
     6541 acagggcgac aggttccaaa ccgcgttcgc ggcagaggtg ttgataccaa tggttgccga
     6601 tggcgacatg gcccacttcg tcgcggtaaa tgatgtccaa tacgccgcag gtttccgaat
     6661 cgccgcgctg cgccaccttc gcgcgtatcc ccggcgtaac gtccagcccg cgcgcttcca
     6721 acacgcgcgg taccaacgcc atacgcaaca gtgggtcgta ggcggttttg tacgccatat
     6781 cccacaaatg gttgtgcgcc tcgaaactgc cgtaatcgaa gccgaaagcg cgcaatctgt
     6841 cgcgcatcag gcggaaatgg tacacctctt ccttcgccac tttcacccag tcgcggacaa
     6901 actgaaacgg cagcgtgcgg aaacggtatg ccgcgtccaa agccagattg acggcgttga
     6961 actcgatatg cgcaatcgcg tgcagcatcg ccgcatagcc ttctgccgtg ttcattttgc
     7021 gcggggtcag ttgtgacggc gcgaccaaaa ccggcttgtc cggccgtccc gcgcggggga
     7081 aatctgccgg tggcgcgttt gtttccgccc cgtccgcatt ttgaacggcg gcaaacgcct
     7141 catccgtcag ccgtcctttt tcatcggggt cgcccgaaag cagggcgcgt tccagcaaag
     7201 cataaatatc gggtttcatt tcaagtccgc cgtgttcgga aaacaaatat tatagcgttt
     7261 aaaaaaaaac aagatgaggc atataatctc cgcgattcgg cattccgcgc ccaaaccgtc
     7321 aaatcacgcg gcacacaatt aaagggcagc gtggcgcgcc taccttttcc gagcggtcaa
     7381 aaaaatcagc cctcggaaaa cgctgtttgt aaaatgcaaa ccgcccgtaa cgccgcccgt
     7441 atgattgttt tgccgcgccg atactttacg ccacacccat cccaacaagg aaaaataatg
     7501 atgaaaccgc acaacctgtt ccaatttctc gccgtttgct ccctgatcgt cgccgtcgct
     7561 tccgcacagg cgggcgcggt agacgcgctc aagcaattca acaacgatgc cgacggtatc
     7621 agcggcagct tcacccaaac cgtccaaagc aaaaagaaaa cccaaaccgc gcacggcacg
     7681 ttcaaaatcc tgcgcccggg cctcttcaaa tgggaataca cttcgcctta caaacagact
     7741 attgtcggcg acggtcaaac cgtttggctc tacgatgtcg atttggcaca agtgaccaag
     7801 tcgtcccaag accaggccat aggcggcagc cccgccgcca tcctgtcgaa caaaaccgcc
     7861 ctcgaaagca gctacacgct gaaagaggac ggttcgtcca acggcatcga ttatgtgctg
     7921 gcaacgccca aacgcaacaa tgccggctac caatacatcc gcatcggctt caaaggcggc
     7981 aacctcgccg ccatgcagct taaagacagc ttcggcaacc aaacctccat cagtttcggc
     8041 ggtttgaata ccaatcccca actctcgcgc ggcgcgttca agtttacccc gcccaaaggc
     8101 gtggacgtgt tgagcaactg atgccgtccg ccccgatgcc gtctgaaagc cgccgaggct
     8161 tcagacggca ttttcacgca ggcggaacaa tgtcccgcat taccgcccga tcgggcatcg
     8221 gaacggcaaa cccgtgaaaa ttaacggttg cgccccggct gtttttgccg tttaatgcaa
     8281 accttgctgc accaagggcc aagaaagccg accggccgcc cccacagctt ccgatgcagg
     8341 cggcccggcc gtccctgcaa tgttttttat ttttgaacga aaggtcgaaa accatgaaaa
     8401 aaacactggt ggcggcggca atcctgagcc tcgccttgac tgcgtgcggc ggcggaaacg
     8461 ataccgccgc ccaagccccc tccgccaagc ccgaagccga acaatcgggc aaactcaata
     8521 tctacaactg gtcggattat gtcgatcccg aaaccgtcgc cgcctttgaa aaagaaaccg
     8581 gcatcaagac gcgttccgat tattacgaca gcaacgaaac actggaggca aaagtcctga
     8641 ccggcaaatc cggctatgac ctgactgcgc cgtccatcgc caacgtcggc cggcaaatca
     8701 aagcgggcgc gtatcagaaa atcgacaagg cgcaaatccc ccattacggc aacatcgata
     8761 aagatttgct gaaaatgatg gaagccgtcg atccgggcaa cgaatacgcc gtcccctatt
     8821 tctggggcat caacaccttg gcaatcaaca cgcggcaggt gcaaaaggca ttgggtacgg
     8881 acaagctgcc cgaaaacgaa tgggatttgg tgttcaaccc cgaatacacc gccaaactca
     8941 aatcctgcgg catcagctat ttcgacagcg caatcgaaca gattcccttg gcgttgcact
     9001 atttgggcaa agaccccaac agtgaaaatc ccgaagacat caaagccgcc gtcgatatga
     9061 tgaaagccgt ccgaggcgac gtgaaacgct tcagctcttc cggctatatc gacgatatgg
     9121 cggcgggcaa cctgtgtgcc gccatcggtt acggcggcga tttgaacatt gccaaaaccc
     9181 gtgccgaaga agccgcaaac ggcgtggaaa tcaaagtatt gaccccgaaa accggcgtgg
     9241 gcgtgtgggt ggattccttt atgattccgc gcgacgcgca aaacgtcgcc aacgcccacc
     9301 gctatatcga ctacacgctc cggcccgagg tggcggcgaa aaacggcagc ttcgttacct
     9361 acgcgcccgc cagccgtccc gcgcgcgagc tgatggatga aaaatacacc tccgacgcat
     9421 cgattttccc gaacaaagaa ctgatggaaa aaagtttcat cgtatcgccc aaatccgcag
     9481 aatccgccaa actgggcgtg aagctgtggc aagggctcaa ggccggaaaa taaccgcacc
     9541 ccctgccgtc tgaaaccttt cgggcggcag gaaacagcgc gtccgttatc aaacaggggg
     9601 gcgtttcccc tcctgccggt tatgattggg ttaagattaa aatgatttag taaaatgaga
     9661 aagatatgga tttaagtatc gtagttccca tttataatgt cgaaagttat ttggaagcgt
     9721 gtttaagttc catagaacct atattaagta atgaaaatgt cgaacttatc cttgtgaatg
     9781 acgggtcaaa agacggaagt gaagatatat gttataaata tatagataaa acaacaaaca
     9841 acaaacaaca cccgataccg aacaccaaac atcaaatata tatatcagga taaccaagga
     9901 ttgtcggggg cgagaaatac cggaataaaa aattcaaacg gaaaatatat agtctttatt
     9961 gattcagatg attttattaa ttgtcaggtc ttgctggatt ttcttagtaa agatgatatt
    10021 gatatgccgg atgtggtttt tttaaatgca gttaaatatg ataagggaag tgtttcacat
    10081 tttggcgaag attatcagcc tgaaaaaata ctcaatcaat ccaaagtcga agttttgaaa
    10141 ggattatgcc gatttagaaa atttccgggt tcggcgtgta ataagattat aaaaagggaa
    10201 ttgattatca aagaaaaact gttttttgaa aaagaggttt atgccgaaga tatcgaatgg
    10261 tcaatgagat tatttaatgc ggcaacaact ttttcttatt tggacggttg ttattactat
    10321 tatcggcagg gaagaaaaga ttctaaaaca ggaactgttt cggaaaaaag tataaagtca
    10381 ttattatata ttttggagaa aaatgcggaa atggaatttg atagggatat atcaagttat
    10441 ctttattctt ttctttccta cgaatatctc gttttgcttt ttatcatgac gagtaaaaat
    10501 atagagtgtg atgctgatat aaaaagaagg gcgtatcatt taaggtttat gctgttaaag
    10561 tccaataaat tgatatataa gctgatattc ccgataatca cattattcgg ggtcgatatt
    10621 acaggcagga ttttaaaagc aatcaggggg aatatttaat aaatccttta acactataca
    10681 tcttaccgaa ggaggaaaaa tgaacgcaat ccgaactttc caaaaccgca cgcccgaaat
    10741 ccacgaaagc tgtatgatag acgaagcgtg cgtcgtcatc ggcgaagtgt cgcttgccga
    10801 agacgtttct gtgtggccgt gcgccgtgtt gcgcggcgat gtgaacagca tcaccgtcgg
    10861 cgcgcgcagc aatatacagg acggcagcgt cctgcacgtt tcccacaaaa ccgccgccaa
    10921 acccgaagga tcgccgctgg ttatcggcga agacgttacc gtggggcaca aagtgatgct
    10981 gcacggctgc cgaatcggca accgcgtctt ggtcggtatg ggcagcaccg ttttggacga
    11041 tgctgtgatt gaggacgaag tgatgatcgg cgcgggcagc ctcgttccgc cgcgcaaacg
    11101 cttggcgggc ggctaccttt atatcggttc gccggtcaga caagtgcgcg tgctgaccga
    11161 tgaggaaaaa gcctttttga aatattccgc cgcgcattat gtgaagctgt cgaaacagta
    11221 cgggatgtga aatcacatcg gcgttcttgc gtcagcccca aattcatgcg gacgggatgc
    11281 atccgataac ggtatccgat gcgccttgat tttgaccgtc tgcgtttgag ttgcaggcaa
    11341 aaatgccgtc tgaagccctt ttttcgggtt tagacggcat tttatcgccg attgcttttt
    11401 acagtttgac cgaatgttcg cgcgtttcgt ggaacacgat gtccggccaa cgttcttgcg
    11461 tcagccctaa attcatgcgg acgggatgcc cgataacggt atccgatgcg ccttgatttt
    11521 gatcggtgca tttgagttgc aggcaaaaat gccgtctgaa gccctttttt cgggttcaga
    11581 cggcatttta ttgccgattg ctttttaaag tttgaccgaa tgctcgcgcg tttcgtggaa
    11641 cacgatgtcc ggccagcgtt cttgcgtgag tcccagattc acgcggttag gggcgaggta
    11701 ggcgaggttg ccgcccgcgt cgatggcgag gttgcctgca ttggcttttt caaattccgc
    11761 cagttttttc ttgtcgtcgc acgataccca gcgcgccgac cagatggatg cgctgtcgaa
    11821 cacggcttct acgccgtatt cgttggccag ccgcgaggta acgacttcaa attgcaacac
    11881 gccgaccgcg cccaaaatca aatccgcgcc gctcatcggt ttgaaaacct gtaccgcgcc
    11941 ttcttcgccg agttgttgca aacctttttg cagttgcttg attttcagcg ggtttttgat
    12001 gcggacgctg cggaacagtt cgggcgcgaa gaatgggatg ccggtaaacg tcagttgttc
    12061 gccttcggag aagctgtcgc cgatttggat gttgccgtgg tttgggatac cgataatgtc
    12121 gccggcgtag gcttcttcaa ccagctcgcg gtcgtgggac atgaaggtta ccacgctgga
    12181 ggcggcgatt tcgcggttga tacgcaggtg tttcatcttc ataccgcgct cgaatctgcc
    12241 ggagcagacg cgcaagaagg cgatgcggtc gcggtgtttc ggatccatat tggcttggat
    12301 tttaaagata aatccggaaa acttagcctc gtccggctcg accatacgta cggtcgcgtc
    12361 gcgcggtttc ggcgcgggcg cccagtcaat taatgaattg aggatttcct gaataccgaa
    12421 gttgttaatc gcagagccga agaatacggg cgtgagttcg ccggcgagga attcgtcgag
    12481 attaaactcg ttggaagccg cctgcaccaa ttcgatttcg tcgcgcaact gttggatttc
    12541 caatggaaag cgttgttcca attcgggatt gtctatgcct ttgatgatgt cgaactcgtg
    12601 cggcaggcgt tcgccgcccg cttcaaagag atagatttcg tcgttcagga tgtggtacac
    12661 gcctttgaag tttttgccca taccgattgg ccaggtgacg ggcgcgcagc ggatttgcag
    12721 gatgttttcc acttcgtcca gcaattccag agaatcgcgt acttctcggt cgtatttgtt
    12781 catgaacgta acaatcggcg tatcgcgcag gcggcagacg tttaagagct tgatggtttg
    12841 cgcttccacg ccttttgccg cgtcgatgac catcaacgcg ctgtccacgg cggtcaaaac
    12901 gcggtaggtg tcttctgaga aatcttggtg tcccggcgtg tccaagaggt tgacggtgtg
    12961 gtctttatag tcgaactgca tcacgcttga tgccacggaa atgccgcgct gcttctcgat
    13021 gtccatccag tcggaagtgg cgaatttgcc ggtttttttg ccttttaccg tacccgcgct
    13081 ttgaatcgca cctgaaaaca gcaagagttt ttcagtcaac gtggttttac ctgcgtcagg
    13141 gtgggagatg atggcaaacg tgcggcggcg gcgcacttgg tcgaggattt cttgggacat
    13201 ggttttcttt gcaaaaaggt tcaggccgct tttcagacgg cccggacagt gtttgagacg
    13261 gcgaaattgt acaaaaaaat gcctgataat tcaatgttgg aggcggtcag tgcgtgctgc
    13321 cgtaaatctc tttttcgtct ttcaggacgg catcggcggt ttcccacgcg ctgccacgcc
    13381 agactttgta aaagcagctt tctcgcccgg tgtggcaggc gatgccgccg ttttgggcga
    13441 tgagcatcac aatggcgtcg ccgtcgcagt cgaggcgcag ttcgcggact ttttgcgtgt
    13501 gtcccgactc ttcgcccttc atccattgtt tttggcgcga acggctgtaa tagtgggcaa
    13561 agccggtttc gacggttttt tgcagggctt cggcgttcat ccacgccacc attaaaacac
    13621 gtttggtttc ggcatcttgg gcgatggcgc aaaccaaacc tttttcgtca aatttgacgg
    13681 cttcaagcag gtttttatcc atattttctt tcagacggca tggtcggggc ggtcagaggc
    13741 gcacttcgat gccggcttcg cgcatagcgc gtttggcttc gcggatggcg atttccccga
    13801 aatggaaaac gccggcggca agtacggcat cggctttgcc ttcggttatg ccttcaatca
    13861 ggtgccggac attgccgacc ccgccggagg cgatgacggg gatgtcgacg gcttcggcaa
    13921 cggcgcgggt cagcggcagg ttgaaaccct gtttcgtacc gtccctgtcc ataccggtaa
    13981 gcaggatttc gcccgcgccg cgtttttgca tttcgatcgc ccattccacc gcatccaaac
    14041 cggtcggatt gcgcccgccg tgggtaaaga tttcccagcg tgtgtttccg gggttgacgg
    14101 ctttggcatc gacggcggcg acgatggctt gcgaaccgaa aaatccggcg gcttcgtcaa
    14161 tcaggtcggg acgggtaacg gcggcggtgt tgatgctgac tttgtccgcg ccggcattga
    14221 gcaggcggcg gatgtcggca acggtgcgta cgccgccgcc gacggtcagg gggatgaaga
    14281 cttgtccggc aacctcttcg atgatgtgca ggatggtgtc gcggttgtcg gatgaggcgg
    14341 tgatgtcgag gaaggtcaat tcgtccgcgc cttcgccgtt gtagcgtttg gcggcttcga
    14401 cggggtcgcc cgcgtcgcgc aaaccgatga agttcacgcc tttgacgacg cgcccgtctt
    14461 ttacgtcgag acaggggatg atgcgttttg ccagtgccat aatcggatgc ctttagtcga
    14521 gggaatctgc cagttgctgc gcttgggcaa aatcgatgct gccctcgtaa atcgcgcggc
    14581 cggtaatcgc gcctgctacg ccatgttttt cggcggcaca cagggcgcgg atgtcgtcca
    14641 agccggtcag tccgccggag gcgatgacgg gaatgcggac ggcttgggcg agtttgaccg
    14701 tcgcgtcgat gttcacgccg ctcatcatac cgtcgcgccc gatgtcggtg tagatgatgc
    14761 tgttgacgcc gtcgtcttca aagcgttttg ccaaatcaat tacatgatgc ccggttacgg
    14821 ttgcccagcc gtcgatggcg gccataccgt ctttggcatc cagcccgaca ataatcctgc
    14881 tggggaaggc tttgcacgcc tcgcgcacga agtcggggtt tttgaccgcc gccgtgccga
    14941 taatcacgtc gttcagaccc aaatccaaat attgtccgat ggttttcaaa tcgcgtatgc
    15001 cgccgccgag ctgtacgggg atgtctttgg cgacggcggc aaggatgtct ttgatggcgg
    15061 gcaaattttg cggcacgccg gcaaacgcgc cgttcaaatc caccagatgc aggcggcgcg
    15121 cgccttgttc aaaccagtgc agcgcggtgt cggcgggcga atcggaaaag acggtcgcct
    15181 cttccatcag cccttgtttc aggcggacgc agcgtccttc tttcaaatcg atggcgggta
    15241 tcagcagcat agtttttctc cttgtgcggg gccgtgtccg gcttaccagt ttaaaaagtt
    15301 tttcaacatc gtcagcccgg catcgtggct tttttcggtg tgaaattgcg tggcgaatac
    15361 gttgtctttg ccgacgatgc aggcaaacgg ggacgggtag tcgctttcgc ccaatatggt
    15421 ttcgggattt tcggggtcga aatagtagct gtggacgaag taaaaacgcg tgtcttgggg
    15481 aatatcttta aacagcgggt ggttttgggt ttggcgcacg gtgttccagc ccatatgcgg
    15541 gactttcaga cggcatccct gccggtcgcg gaggtcgcgc tcaaagcgtc tgactttgcc
    15601 gccgaaccag cccaagccgt cggtgtttcc ttcttcgctg tggtcgaata aaagttgcgc
    15661 gccgacgcag attccgaaaa agggtttgtt ttttaaggca tctttgactg cctcgtccaa
    15721 gccgccgcgc gtcagtgccg caatacagtc gggcatcgcg ccctgcccgg gaaaaatgac
    15781 tttgtcggcg cgggacacgc gttcggggtc gccgcttaaa aagatttcgg tattttttcc
    15841 ggcaagctgc cccgccgtcc ggacggattt caatacggaa tgcaggttgc ccataccgta
    15901 atcgataatg gcgatttgca tggcttcctc ctcttttttt gcaatatggc tgcgatttta
    15961 acaaacaaat gtgccgtgct gataaaaatg ccgtctgaaa acgggagtct gtcttcagac
    16021 ggcatagggt ttaaacccgg aaagccgttt gtcagccttc catttgtttt gcctgaacgg
    16081 cagttagggc gatggtgaac acgatgtctt ccaccagcgc gccgcgcgac aggtcgttga
    16141 ccggtttgcg caggccttgc agcagcgggc cgacgcttaa gacgttggca ctgcgttgga
    16201 cggctttata ggtgcagttg ccggtgttca ggtcggggaa gaccaaaacg gttgcctgtc
    16261 ctgccaccgg gctgcccgga gctttggatt tgcccacacc cggcacggtt gccgcatcat
    16321 attgcagcgg gccgtcgatg gcgaggtcgg ggcgtttttc ccgggcaagt ttggttgctt
    16381 cgatgacgat atcgacatcg gggccgctgc cggagttgac ggtggagtag gaaatcatcg
    16441 ccactttcgg gtcgatgccg aaggcttttg cggaatcggc agactggatg gcgatgtcgg
    16501 caagctgttg cgcggtcggg ttcggattaa ccgcgcagtc gccgaagacg aggacttggt
    16561 tgggcagcag cataaagaat acgctggaca cgaggcttgc gcccggtgcg gttttaatca
    16621 gttgcaaagc ggggcggatg gtgttggcgg tggtgtgaac cgcaccggat accaaaccgt
    16681 ccacatcatt ttgcgccatc atcatcgtac cgagtaccac ggtgtcttgc agttgcttgc
    16741 gcgcgtcttc gggtgtcagg cctttggatt tgcgcagttc gcacatcggt ccgacgtatt
    16801 gttcgaccaa tgaggcggga tcgatgattt ccaaagagtc gggcaggctg ataccgcgtt
    16861 ctttggcaac ggcttcgact tcttcgcgtt tggcaagcag gacgcagcgg gcaatgcctt
    16921 tttcgtggca gatggcggcg gcttggacgg tgcggggttc tgcgccttca ggcaggacga
    16981 tgcgtttgtc ggcttggcgg gcgaagtcga tcaggttgta gcggaattgc gccggcgaca
    17041 ggcgttttgc ttcgcggcct gccaatacgg atacgtcttt cagcgcgtcg ctcgaaccga
    17101 agaaggtcag gccggttttt tcggctgccg cttcggcaac ggaggctgcc gcgccgtcca
    17161 cgacaaaacc ttccaatacg cccggcgcgg cggcgaagaa ctgtttggca aggttcaccc
    17221 gatttgccag ttcgtcggca tcggcgttgt cggaacggac ggcgaagacg gctgccgcgt
    17281 caagggacaa tgccagttcg acgtttttgc ctgcgaggta gattttgtcg gcatcgggcg
    17341 cgatgccttc gatgacgagg ttggcggcat cgagtgcggc aactttgccg accagtgcgt
    17401 cgaaccagtc gtcgcttttg ccttgcgcga gcagggtttc ggcggttgcg tcaacggctt
    17461 ggaaaatttg tgcgtccagt gcttttgcaa aggcttgtgc ggcggcggag gcgtccagtc
    17521 cggcagatac gggtacgatg agtacttttg ccatgatata tcctttcgta tgctgcggtg
    17581 tgcggcatat gtggttggaa ggggcggcat ataggcagaa acggctgcct gcgtgccgtg
    17641 cgtgccgtgt ttggcttgag gcgcgcaggt tgaatatagc aaacaaattc tgtttccaac
    17701 aagataaata tccgcaggct tgtggatgct gccgcctttc agagggtatt tccggggaag
    17761 aacagggcgg gaccgtccaa atggaggacg gcggaaatgc cgtctgacag ggtgggggcg
    17821 gaagggaggt tgagcgtgag gacggtttgt ccgacccgga gactgatttc ggtatgccgc
    17881 gctttgggcg tggttttgag aaccacggcg tgaatggagg cggtgggtgc ggaatggggg
    17941 tgaaggctga actgttccgg acggatgagc agtgtgccgc gtgtgcctgc gggtgcgccg
    18001 ctttgaacgg gcaggcggcc caatccgcaa tcggcggtgc cgtcggcgtt gagcgcggcg
    18061 gggaacacga tgccttcgcc gataaacagg gcggcatcaa ggtcggcagg ttgtcggtac
    18121 aattcgtgag ggcttgcagt ttggaggatg cgcccctgtt tcatcacggc aatccggtcg
    18181 gcgtattgca gggcttcttc gcggtcgtgg ctgacgaaaa cggcagattt gccgttggcg
    18241 cgcagggcgg caatcatgtc ttcgcgaatc tggcggcgca actgttcgtc cagcgcgctg
    18301 aagggttcgt ccaacaaaat cagttcggga tcgggtgcga gggcgcgggc gagggcgacg
    18361 cgctgttgct gtccgcccga aagttcgtgc ggatagcgtc cggcaagttc ggaaatgccg
    18421 gtcaattcca acatagcttc gatgcgctgc cgctcttgcg ccgtcttgcc tttgccgttg
    18481 cccagcccgt aggcggtgtt gcggtaaacg gtcaggtggg ggaacagcac accttcctgt
    18541 acgacataac ccaaacggcg ttcgcggacg gggaggttgg tatttttcga gaagatggtt
    18601 ctgccggaaa gcgaaatttc gccaaaatcg ggttgttcaa aaccggcaag gcagcgtaaa
    18661 agggtggttt tgccgcagcc ggacgcgccg acgataaaga ggatttcgcc cgggtcgagg
    18721 ctgagcgaaa tgtcgtttaa aaccggggtg ttttgaaaac ttttggacag gtgtccgatg
    18781 tgcagggcgg cggtcatggc ggtacttcct caagctgtta tttgaaggcg tatttcttca
    18841 gcaggaatac ggggatgccg gaaaataata ccagcatcag cgcgtaaggg gtggcggcgg
    18901 cgtattgtgc gtccgatgtg tattcccaaa cggcggtgga gagtgtgtgg acatcgtcgg
    18961 tggtcagcag cagggtggcg gtcagctctt tcatcagttt gaggaagacg agtgcgaatg
    19021 cggcggtaat gccgggcagg atggacggca gtaccaacgt cctgaaaata aagaagtgtc
    19081 cgcgccccaa tgttgcgccg acctgttcca tccctttggg gagttgttcc aaggaagtcc
    19141 tcagggtggt ttgcgccatc ggcaggtaaa gcatgaaata ggcaaggatg acgacgataa
    19201 aggtttggta aacggcaggg gtgtagttga tgctgaaata aaccaaggat agggcgataa
    19261 ccaaaccggg gacggcgtgc agtaaaaacg gcagcctgtc tatccaaacg gttaaaaaat
    19321 tgcgatagcg aaccgatgcc caaacaaggg gcaaggcaca taatatagtc aaaatcgcac
    19381 ctaaagccga tacgcttaag gaacggataa aggcatcaaa tacggatacg agcgcgaatg
    19441 tgccggaagt gccgaccatc atccaatgta tcaatacgcc aaaggggata ataatgccca
    19501 aagtcaacaa gctgcttaaa aaaacaatcg cgccaatctg accgggcagt ttgagggttt
    19561 tgacgggata aggacgggca acgcctttgc cgctgtggta aatcttggct ttgccgcgaa
    19621 atatgctttc tccaaatacg acgatgccgc acaccgccat taaaacagcg gaaagcaggg
    19681 cggcggtatt gttgttgtag gacatttcgt attcttggaa aatggcggtg gtaaaagtgg
    19741 ggtagttcaa aatagatacc gcgccaaatt cgaccagcat atgcagggca atcagtaaca
    19801 cgctgctgcc gatggcgggt ttgagctggg ggaggatggc ggaaaaaaag gtttgcaggc
    19861 ggcttttgcc caaggacagg ctgacttctt cgtaagacag gctgatgcgt ttgagtgccg
    19921 cctcgacggg caggtaggcg agcgggaacg aggacaggct cataatcatc actgtccccc
    19981 aaaagccttc gacacggaag gtcaggctga tccaggtgaa acagctgaca aatgcgggga
    20041 tgcacaaagg cagggtgatt gccgtctgaa aaaaggtttt gccgaagaag cggtaacgtt
    20101 ggaacaaaag ggcgcaggca atgcccaaaa caatggaaat cagggtaacg cccgccatca
    20161 tcgtcaaggt gttggagagc aaatcccaca tacgcgggcg gaacaacagt tcgacggcgc
    20221 ggttgatgcc gacctgccac gaacgcatag cgacatataa aaaaggcagg gtaagcggta
    20281 gggcaatcag taggatgagg ccggtaagcc aaatgggtat ttttttagga gacatagtgt
    20341 tttttatcgg caaaacgggc ggacagtata aatgtccacc cgtttgacaa tccgaaaacg
    20401 gcttatttca taccggcttg ctcaagcagc cgggtggcgt gttctttttc ggaaacagtg
    20461 gtggcggaca cttggggtgc ttccaacttg gcgatgggtt ccaaattgaa agtggatacc
    20521 acgtgcggat tcaaaggata ttcggcacgg acggcggtca gggcgcgctg tccttccttg
    20581 ctggcgagga aggcgacgaa ttttttcgcc tcatccttgt tttgggagga ttttaacacg
    20641 gctgcgccgg aataggtaac gagtgcgccg ggatctctgt ggcggacgaa attcaggcgg
    20701 gtgtggacat tttgtacgcc tttttcacgc gcaaaagcgt gccagtagta gttgttgatg
    20761 agggcggcat cgatttcgcc gttttcaacc gcttgaaggg cgacggagtt tttagcgtaa
    20821 ggcttgccgt attctttcag acctttgagc catttcaatg cggccgcttc gcctttcagt
    20881 ttgacgatgg cgacaacctg ttccaagaac gcgccggaag tgggggcgta accgatgcgg
    20941 tttttccatt tcggcgtggc gtaattcagg acggattttt ccaaatcttt ttcagacagt
    21001 ttgcgggtgt cgtaaacgac gacgcgcgaa cgtccgctca gtgccaccca gtcttttttg
    21061 gcggcaaccg gcacgccctt gccgcgtgtt tcgttgatgg tggaggcggg caggggctct
    21121 aggaggttgg ctgcggaaag ggtggcgagt gccgggattt gttcggaata gaatacgtcg
    21181 gcggggcttc ggctgccttc ttctttgatt tgaccggcaa gctggtcgcc tttggcgctg
    21241 ttgagtttga ctttgatgcc ggtagcccgg gtaaaggcat ctgcaacggc ttgtgccgct
    21301 tctttgtgtt ggccgttgta cacggtaatg tctgccagcg cgggggttgc ggcggtcagg
    21361 gctgcggcaa gcagtgcgta tcggatagat gttttcatat cgattttctc ctaatgaatg
    21421 agagtgtaca ccttgttaag acataacggg gtgtagtgta ttccttcttc tttataaatg
    21481 caaataatta ttttttaaat ttgttattat ccgatccggt tattgtttgt tctgacttgt
    21541 attttttccg tgcatcgcgc ccgtaaggcg gaagcggcgg gcaatgcgtg gcggaatgtg
    21601 ggtaaaggcg gcattttgat ttgtcggaat gcttgagaac ccctctcttt aaaacagcgt
    21661 ctgaacaggg ttgccggaat agtattgcca tcccggcaga tacagtttgt cggggtcttg
    21721 ccaatattgt tcatccagac tgttcggcag tgaggcggtt ttgttgtcga gatgttttgt
    21781 tatatcccac atttctttca ggtttttacc ttccgattgg aggtggcgga tttcttttct
    21841 caatgcgtct gccgcccgat ggatcagcat tgcgctgtgt atcgcgccga tttttttcag
    21901 aacggaacaa gtcttttcgg cggcgggaaa accccaattg cagacaaatt gcagtatgcc
    21961 gccgttaccg atatcggctt ctgtccgcca aatcaaaacc agctcctgtt cccgcccgtc
    22021 catgctttcg agtttgccgt catgctgttc aaaaagtttg tccaccgcct gccacatcat
    22081 ctgttcgaag cggtcggctt gggtatccgt atcggtcatc gtgttcttgc cttttaaaaa
    22141 tgccgtctga acatttcttc agacggcatc gggggttaag ctgacatttc ccgccagcgt
    22201 ttgacttgga aacggacttg ttcaggcgcg gtaccgccca agtggttgcg cgcgtttaag
    22261 ctgccttcgg gtgtcagcac gccgtaaacg tcgtcggcaa tcaaatcgct gaaaccttgt
    22321 aagacttcga gcggcagttc gctcaaatcg acgccagctt catcggcgtg gcgcacggct
    22381 tgggcgacga cttcgtgggc atcgcggaag ggcatacctt ttttaaccag ataatccgcc
    22441 aagtcggtgg cggtggcaaa accctgcatc acggcggcgc gcatattgtc gggtttgacg
    22501 gttacgccgc gcatcatatc ggcgtaaatc cgcagcgtgt cgatgagcgt gtcggcggtg
    22561 tcaaacaaag gttctttgtc ttcctgattg tctttgttgt acgccaaggg ctgggatttc
    22621 atcagggtaa tcaggccgat aaggtgtccg atgacgcgtc cggatttgcc gcgcacgagt
    22681 tcgggcacgt cgggattttt cttctgcggc atgatggacg aacctgtgca gaaacggtcg
    22741 gcgatgtcga taaagccgaa acgcggactc atccacaaaa tcaattcttc ggacaggcgg
    22801 ctcaggtgaa ccataatcag cgaggcggcg gcggtgaact cgacggcaaa atcgcggtcg
    22861 gatacggcat cgagcgagtt ttggcagatt tgctcgaaac ccagtaattc ggcggtaatt
    22921 tcgcgccgaa tcgggtaggt cgtaccggca agggcggctg cgccgagcgg catacggttg
    22981 acgcggcggc ggcagtccgc catccgttcg ttatcgcggc cgagcatttc gacgtaggcg
    23041 agcatatggt gtccgaagct gacgggctgg gcgacttgca ggtgggtaaa gccaggcata
    23101 acggtttcgg cgttttgttc cgccaaatcc agcaatgccg tctgaaggtt ttgaatcagg
    23161 ttttgaataa cggtaatctg gtcgcgcagc caaaggcgga tgtcggtggc gacttggtcg
    23221 ttgcggctgc ggccggtgtg caggcgtttg cccgcgtcgc cgattttgtc ggtcaggcgg
    23281 cgttcgatgt tcatgtggac atcttccaaa tcggacgacc attcgatttt gccgctgcgg
    23341 atttcttcga ggatttccgc catgccccga cggatgtctg ccaaatcgcc ttcgtccaac
    23401 acgccggttt ctttcaacat ttgcgcgtgt gccagcgagc cttgaatgtc ccattcggca
    23461 agccgtcggt cgaaaccgat ggaggcggtg tattgtttga cgagttcgga aacgggttcg
    23521 ttgaaacgtc cggaccaggt tttgtcgtgc ataaggattc cttgatgggg ttattcggta
    23581 cggtattttt ccaaaaggcg gcggaagggt tcgccggttt cgggatgttt cagaccgtag
    23641 gcgacgatgg cttcgaggta gcccagtttg ctgccgcagt cgtagcgcgt accttcaaag
    23701 gggtgcgcca ggacaaattc gtgatcgagc agcttggcga tgccgtctgt aagctggatt
    23761 tcgttgcccg cgccgcgcgg aagattggtt aagaggtcga aaatgcgcgg cgtgagaatg
    23821 tagcgtccga cgacggcaag gttggagggc gcgtcttcgg gcttgggttt ttcgacaatg
    23881 ccggtaatgc gttggaactg tttgagatgt tcggtttcga cgatgccgta tgagccggtt
    23941 tgcgacggtt cgacggtttc tgcgcccaaa atgctgttgc cgctgcgttc gtacacttcg
    24001 accatttgtt tgagcgcgcc ttttggggca tcgattaagt cgtcagccaa aatcacggca
    24061 aagggttcgt cgccgatggc ggcgcgggcg cacaagacgg cgtgtcccaa gcccagcgct
    24121 tccgcctgac ggatgtagag gcaggtgatg ttcggtggca ggatgttgcg gacgtgttcc
    24181 agaagtttgt ctttatggcg catttccaac tcggtttcga gttcgtatgc cttgtcgaaa
    24241 tggtcttcga tgctgcgttt gttgcgtccg gtaacaaaca ccatttccgt gcagccggct
    24301 tccacggctt cttctacggc gtattggatc agcggcttgt cgacgatggg cagcatttct
    24361 ttcgggctgg ccttggtggc gggcaggaag cgggttccca tccctgcgac ggggaagacg
    24421 gcttttttta tcggtttcat tctttttcct ttgtattgtt ttgatgttta aagggcgagt
    24481 ttgtgaatta aatcggcgag tgcctgtgcg cggtggcttt cgcggttttt gacctccgaa
    24541 tccaattcgg cggcggtttt gccgtgtccg ggcagataaa aatacgggtc gtaaccgaaa
    24601 ccgttttcac cggcgggaac gccgttccat tgtccgtgcc atacgccttc ggcaataatc
    24661 ggacgcgggt cgtctttatg gcggacaaaa accaatacgc agacgtagca acagcttttg
    24721 tcggctttgc cggcaagttc ggcggcaagt ttcaggttgt tggcggtatc ggatttggga
    24781 ttgctgccgg cgtaacgtgc ggaatggatg cccggagcac cgtttaaggc ggcggcacag
    24841 atgccgctgt cgtcggcgag tgcgggcagc ccgctgtatt tggcggcatg gcgtgctttt
    24901 gccagcgcat tttcgacaaa ggtggaatag ggttcggggc attcgggtat gccgaatgtg
    24961 gattgcggca atacggtgat gctgtaaggt ttgaataagt tgccgaattc ttcgagcttg
    25021 ccggcattgc cgcttgccaa aacgattttt tccggttttt cagacatagc ggttttcctt
    25081 tgcggcggat tgggcggcgc gtagggattt gtgccgcagg tagagggcga gtgcgccgat
    25141 ttgcccgaac agcgcgccga atgcgaaaag aaaggaggcg acggcgaagg ctttgctgcc
    25201 gacggtcagc agataagcac ccaagaggac gagcagcata aatgccagtg tgaagagggc
    25261 aacagacaac agatagattt ttcggcggtt catggcgttc ggtcggaaac ggtatgttcg
    25321 gattatatcc gattgggacg gtattcccta gagcttggaa aaatgccgtc tgaagcggcg
    25381 ttcagacggc atggcgggtg ttatgcctgt ttgttccaac gttcgatgga ttctttaatg
    25441 acttttttcg cttcttccgc atcgccccac gattcgactt tggtcgtacc tgcttttttc
    25501 aggtctttgt aatggttgaa gtggaactcg atttgtttga tgagctgttg cggcaaatcg
    25561 gacaaagttt tgtaggcgtt gccgttgttg cggtcgtcgg caggaacgca gacgattttg
    25621 tcgtctactt cgccgtcgtc aacgaatttc atcacgccga taacgcgcgc ttccaagaat
    25681 acgccggttg ccaaaggttg ttcggtaacg agcagcacgt ccaattcgtc gccgtcttcg
    25741 tccaaagttt ggggaatgaa gccgtagttg gtcggtttgg cgaagatggc gggttcgacg
    25801 cggtcgagtt ggaatgcggc cagtttgcgg ttccactcga ttttgtggtt gctgccggcg
    25861 gggatttcgt tgacgacgtt gatgatgccg ccgtccacat cgccgggggt caggatttgg
    25921 ttgaagtctg ccatttggtt tcctttgttt gggaaagttt gaagtttgaa agtatagcac
    25981 aaacgtccgg ctggaaatgc gcccgatgcc tctgaaaggg tgtacgggcg cgtgttaccg
    26041 tttgcccaaa aacctgccca gttccaaaat cgcgcgcctg ttggacgggg aaaatacttt
    26101 ttccgctgct tcttccatat cgaaccaacc gtaggagacg tgttcttcgg gttgcagggc
    26161 gatgggcgta tcgcgcggga tttcggcaga gaagaggtgt tcgcggtttt caaacacgcc
    26221 ttttggatag cggtgccgcc agtggtggta gatttcgtaa accgtgctgt cgtgccagtc
    26281 ttgaagctgc ccgtccgcca gcaggatgcc ggtttcttcc caaacttcgc gccttgccgt
    26341 ttgggcgacg gtttcgcccg gttcgaggct gccggttacc gactgccaaa atcctttcgg
    26401 atgcgtgcgt tcgatgagca ggatgccgcc gtccccgcta taaaggacga ccagtgcgga
    26461 aacggggtat ttgagcggtt ttgccatcgg catctttcgg cgggctgcgg taatgaaggg
    26521 gcggattata gcaaacgcgc acgttatggc gtttatcctt ttccgtatcc ttttttctgc
    26581 acggatggga cgcgccggtg tttgccggta aattttccgt tgtgtcaaaa agataagggc
    26641 ggttgtgatt ttaatgcttg ccaaagcgtc gggcggaaac tataatccga aacttatcga
    26701 gtcggagtgt ggcgcagtct ggtagcgcac ttgcatgggg tgcaaggggt cgaaggttcg
    26761 aatcctttca ctccgaccaa aaattccgaa agccgctttc aaaagcggct tttttgccgt
    26821 ccgtatgatt atgatgtaga gtacgcggcg acagacattc aaatgccgtc tgaaaaccgt
    26881 tcagatggca tctctttatc ttagtttcat tccgtaccat cttaaggaac atcaaattgg
    26941 gcatttcccg caaaatatcc cttattctgt ccatactggc agtgtgcctg ccgatgcatg
    27001 cacacgcctc agatttggca aacgattctt ttatccggca ggttctcgac cgtcagcatt
    27061 tcgaacccga cgggaaatac cacctattcg gcagcagggg ggaacttgcc gagcgcagcg
    27121 gccatatcgg attgggaaaa atacaaagcc atcagttggg caacctgatg attcaacagg
    27181 cggccattaa aggaaatatc ggctacattg tccgcttttc cgatcacggg cacgaagtcc
    27241 attccccctt cgacaaccat gcctcacatt ccgattctga tgaagccggt agtcccgttg
    27301 acggatttag cctttaccgc atccattggg acggatacga acaccatccc gccgacggct
    27361 atgacgggcc acagggcggc ggctatcccg ctcccaaagg cgcgagggat atatacagct
    27421 acgacataaa aggcgttgcc caaaatatcc gcctcaacct gaccgacaac cgcagcaccg
    27481 gacaacggct tgccgaccgt ttccacaatg ccggtagtat gctgacgcaa ggagtaggcg
    27541 acggattcaa acgcgccacc cgatacagcc ccgagctgga cagatcgggc aatgccgccg
    27601 aagccttcaa cggcactgca gatatcgtta aaaacatcat cggcgcggca ggagaaattg
    27661 tcggcgcagg cgatgccgtg cagggcataa gcgaaggctc aaacattgct gtcatgcacg
    27721 gcttgggtct gctttccacc gaaaacaaga tggcgcgcat caacgatttg gcagatatgg
    27781 cgcaactcaa agactatgcc gcagcagcca tccgcgattg ggcagtccaa aaccccaatg
    27841 ccgcacaagg catagaagcc gtcagcaata tctttatggc agccatcccc atcaaaggga
    27901 ttggagctgt tcggggaaaa tacggcttgg gcggcatcac ggcacatcct atcaagcggt
    27961 cgcagatggg cgcgatcgca ttgccgaaag ggaaatccgc cgtcagcgac aattttgccg
    28021 atgcggcata cgccaaatac ccgtcccctt accattcccg aaatatccgt tcaaacttgg
    28081 agcagcgtta cggcaaagaa aacatcacct cctcaaccgt gccgccgtca aacggcaaaa
    28141 atgtcaaact ggcagaccaa cgccacccga agacaggcgt accgtttgac ggtaaagggt
    28201 ttccgaattt tgagaagcac gtgaaatatg atacgaagct cgatattcaa gaattatcgg
    28261 ggggcggtat acctaaggct aagcctgtgt ctgatgcaaa accgagatgg gaggttgata
    28321 ggaagcttaa taaattgaca actcgtgagc aggtggagaa aaatgttcag gaaataagga
    28381 acggtaataa aaacagtaac tttagccaac atgctcaact agagagggaa attaataaac
    28441 taaaatctgc cgatgaaatt aattttgcag atggaatggg aaaatttacc gatagcatga
    28501 atgacaaggc ttttagtagg cttgtgaaat cagttaaaga gaatggcttc acaaatccag
    28561 ttgtggagta cgttgaaata aatggaaaag catatatcgt aagaggaaat aatagggttt
    28621 ttgctgcaga ataccttggc aggatacatg aattaaaatt taaaaaagtt gactttcctg
    28681 ttcctaatac tagttggaaa aatcctactg atgtcttgaa tgaatcaggt aatgttaaga
    28741 gacctcgtta taggagtaaa taaaaatggc aatttggaat tatcggtact accttatccc
    28801 atcagctgct atcagaaata aatttaatgc aaataaagat atcatacttg atgagtatcg
    28861 atctaatggt tttcagaatt ttaatgagaa taaaagtttt gaaaattact ttatcgataa
    28921 tgatgttata ttattatcaa taataaatga agcaaaaaaa cagcttaaat tgaaagaatc
    28981 ttgggataaa gacgcaatca tgttttgtga taattttggt aatagtctta ccgtttggcc
    29041 agatgatata gagtgcgaac ttgatttaag atttgattat actaaattta ttcagaaaac
    29101 cattgattgg gcaataaaat ataattgtct acttgtaata gaaaaaacag gaaatgtagt
    29161 ttcccctaat ataaataatc tgatgtatga aataaaagca tatttggaaa gcaagccgtg
    29221 gcccatatga aacctaaact caacaagtag gatgtgtgcg gaacgcacgt atgcggttct
    29281 caaggtttga gctaagaggc cgtctgaaaa cagaaaaact gtttcagacg acctttcttt
    29341 taaccagttg ccacagcaac cggacaaaag cagcctacct ccacatccat ataggcaata
    29401 caggggagat attttgtaaa ttctacgaat attttacctg ctaaacaggg taggatatgg
    29461 tatgaagcga acattggctt aataaacact atgtcaagat cgaatcaggc tggtactaga
    29521 ttgttgtatt ccaattatgg attgctatat ataacaactg atcattatat ctctgcaaca
    29581 cggtttgtag cttggaaata ggagtataac ttatgcaatt agagattatc ggtagtaaaa
    29641 tttatacgga acaagatttt cataatcaaa tttcaaaaat attttctata caagattatt
    29701 atgggaacaa tcttgatgct ttatgggatt tattaagcac aaatgtagaa cgaccgatta
    29761 ctttggtatg gaaagatgct atgttctcaa aaaatcaatt agaaaatata tttattgaaa
    29821 tcgtaaatgt tctagaaaga gttaagaaac aagatgagga ttatggattc gaagaaaaat
    29881 ttaattatat tttagagtaa gtaaacccta attacattac gtacacaggc ttaaaactcc
    29941 ccagagccaa ttaagcaagc cgtaacccat ataaaactta aactcaacaa gtagcatgtg
    30001 tgcggaacgt acgcatgcgg ttcttaaagt ttgagctaag aggccgtcta aaaacagaaa
    30061 aaccgtttca gacggtcttt gtttaacgcc accgatccag cgggttacaa agcgcagtca
    30121 atgccgctgc gccttatgcc tccgaagcaa taggcagaac atttggacac ggtgaaaaca
    30181 aaaacgaaac cgcccaagcc gtcggacatt tccttttagg agcagctatt gcccgcgtaa
    30241 acggtggtaa ttttgctgcc ggcggctcgg cagcagttgc agctgaaaag gcggcggaac
    30301 atcttgccca acagtataac gacggtaaaa ccgcaatcga tccgcaaaca ggcgagttca
    30361 atgccaacct gctgccggaa catatcaaag aggaaatcaa atcaaagagc ggggtgattg
    30421 catcgctgac gggcgcggcc gtgggcggca cgccggtaga tgcgcaaacc ggaggtgcgg
    30481 tcggacagaa tgcggtggaa aacaacctct atctgacatc ggaagcctta aagaaggaca
    30541 agcagacagc tcgtaaaatt tattccgtca taaaagagca agtcaagcat gaatgcagtt
    30601 ccacaggaag aattaccgaa tgtcgtcaaa atataggacg cattatcgaa tttacccaag
    30661 acaaacgctt tgacagtagg tttaaggact taaaaaaaga atccttatat tacctaaata
    30721 aacatcctga tttagtagcc tcttatttga aggctgaata cgaaaagctg gatagggaag
    30781 acaaaagtat cctgcaccgc tacatctcac ccggggctga aatcgtttcg ggcagtttgg
    30841 gggttgttct ttcaggagta gccggaggcg gatcttgtgc cgagactttc ggcttaggct
    30901 gtgccgccgc tttggttggt gtaacgtctt cctacgatca tgtcattact gggacgaaaa
    30961 acttcggaaa aaaagccagc gagcaacgac cgacgattgc ggttcaggcc ttgaagcagt
    31021 tggggctgtc ggagcaggct gcggaatatg ttcagttctc tatagatttg ttcagtgtgg
    31081 gtaaatcggg gggcggtata cctaaggcta agcctgtgtt tgatgcgaaa ccgagatggg
    31141 aggttgatag gaagcttaat aaattgacaa ctcgtgagca ggtggagaaa aatgttcagg
    31201 aaacgagaag aaggagtcag agtagtcagt ttaaagccca tgcgcaacga gaatgggaaa
    31261 ataaaacagg gttagatttt aatcatttta taggtggtga tatcaataag aaaggcacag
    31321 taacaggagg gcatagtcta acccgtggtg atgtacgggt gatacaacaa acctcggcac
    31381 ctgataaaca tggggtttat caagcgacag tggaaattaa aaagcctgat ggaagttggg
    31441 aaatgaaaac gaaaaaaggt gggaaagtga tgaccaagca caccatgttc ccaaaagatt
    31501 gggatgaggc tagaattagg gctgaagtta cttcggcttg ggaaagtaga ataatgctta
    31561 aggataataa atggcagggt acaagtaaat cgggtattaa aatagaagga tttaccgaac
    31621 ctaatagaac agcatatccc atttatgaat agtaatattt atgaaaaatt aggagattaa
    31681 tgatgaaaag aattaagtgc ttttgtgata aatttccatc aggagataca tttagaatgt
    31741 gtatcattct ggatgactat gataataggg ttgattatta tgtaggaata tatgattaca
    31801 ttacgtctac cttaatgagc gatatttact atcgatccac gattgatgag catttcaaga
    31861 ttatagaatt aatagaaaat aatccaaatg aaatttatga tgatggcggt ggtcaacaat
    31921 tttgcctaga atttcatcat gataaggtca ttttttatca caatgaattt gatgaagaag
    31981 atggttatcc agtattaagc tgttcgctgc atacttttaa aactgcttta attgcttgga
    32041 atgctttttt gcaattgcct aaaagtattc attcggtggt ggagactgtg attgaggaat
    32101 aagcataatt agcttaatga atagaatcag cgatatagaa aggtagaatc agatatatcc
    32161 cacctaaaaa ttactctcct tcagcaccgc taccaaaagg acctaataat ggatatagtg
    32221 ccgggaattt aagcaaaaaa gtcctgactt tagtttacat aaacggcaaa ctcaaattcg
    32281 ggccaagcag tctgccgtct tcggcattga aagcacctgt tcccattgtc ggcaaggctg
    32341 aggctccgcc cggaaaagct gtgcctgtcg gcgaagcccg cattccttca aatccaaaag
    32401 cggtaaaagg aacaaccgtt accctgtcgc aggaacaacg cctagccaac ggagagctga
    32461 taccgaaagg ctctgtggta acgtggtcgg acaatgcggt gaaggttgtg cggcctgatg
    32521 ggaaaaagcg gagatttaat agtcgggatt gggaacagaa ggcattgccg aaaccgcaac
    32581 taccgaaaga ttacagagat gacatcaaca taggtggaaa taatcctaga tttaatcagc
    32641 aacggcatga aaaaatcgaa tcccacaacc caaacagaat agaggagata cgccaacgta
    32701 atgccgtttc ttctgcagaa aggcaggctg atgcattaaa aaggatgcaa acgtctggcc
    32761 gcacccgcct gcctgggcag ccacaaaaag gaaaacccgg cgctgctaga gaaatgtcct
    32821 cttcagacaa tccgaatcaa acagccgagg cttttgcacg tcgatattta ggcaggaaac
    32881 ctacactaat ggaacgagtc gaaggtgaaa aattatacgg aggtaaatgt gtgggctgtt
    32941 ggaaagcgca aatgccggac ggaacctttg tggtgtatag gccagcaggt aatgcttcta
    33001 tgaccaaggg aactactgcg tctgttgata ttaataaccc aacattaaat aacttaaata
    33061 ttaatttaaa aggcaagcca acagagttaa aatttaaatt ccctgctaga taaggatgtt
    33121 ttaatgaatt tagagacagc agaaaatatt caatgcgaat ttgagtattt agcattagat
    33181 ggttattttc caatgcattt tgcatcgcat ggtcaaggaa acaaagattg gcaatttgca
    33241 gttgaattca tatatagatt attaatatgt caattagcaa ctcttgaacc tatcaacttt
    33301 gaaacgaaaa atgatatttt agacttttgc cataatttag caaaacaaag tccttttaat
    33361 aatgataatg aggtgtggta tcagggggaa attgtattaa caaaaaaagg gatagattta
    33421 ataaaggaat atattccaga ggcgtttgaa gagtggaatg gaaagaaatt tgaattaaac
    33481 attcctttca ttaaaacatt aaaaaatata tttgttaatt atgaggtagc atgggatgag
    33541 aataatccac tattccccat tatatcttta aacctcggca cctgataaac atggggttta
    33601 tcaagcgaca gtggaaatta aaaagcctga tggaagttgg gaaatgaaaa aaggaagggg
    33661 cggaaaacct atgacaaatc attctatgtt cccaaaagat tgggatgagg ctagaattag
    33721 ggctgaagtt acttcggctt gggagaaaag aaaggaatta gaaggtcaaa aatggagagg
    33781 tacaagtaaa tctggtgttg aaatagaagg atttacggaa cctaatcgaa cagcttatcc
    33841 actttataat agtaaatgaa gtattgatta aaaaggtgtt tttaaatgta taaaataaaa
    33901 tgtttttatg atcagtttag ttctggtgaa ttatttaact actgccagtt tttagataat
    33961 tcttctaatc aaaagataaa tacacgagga acggtgtatc aatatattat ttatgtttta
    34021 acaggagacc tttatctaca aaaagacata gatgaaaatt tagaatttat ccatcaagca
    34081 gaaaataatc ccaatgaagt ttattcagga ggtggacaag gtttttgctg ggatatttct
    34141 gctgaaaaag tagttttcta tcacaacgaa tttgatgaag aagacggctg gcctgattta
    34201 agctgttcgc tgcatacttt taaaactgct ttaattgctt ggaatgcttt tttgcaattg
    34261 cctaaaagta ttcattcggt ggtggagact gtgattgagg aataagcata attagcttaa
    34321 tgaatagaat cagcgatata gattggactg caaatccacg cttatacgct gtgccatgat
    34381 taacaggaag agagcaactt ggatgggcta gtagggatgg taagcattta aatatatcaa
    34441 ttgatggaaa gattacacac aa
//
LOCUS       DAXHTY010000010        32542 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXHTY010000010 DAXHTY010000000
VERSION     DAXHTY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.010"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(93..1151)
                     /gene="trpD"
                     /locus_tag="ACFNMK_000418"
     CDS             complement(93..1151)
                     /gene="trpD"
                     /locus_tag="ACFNMK_000418"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC6363749.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(1215..1805)
                     /locus_tag="ACFNMK_000419"
     CDS             complement(1215..1805)
                     /locus_tag="ACFNMK_000419"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC6363750.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            2227..4503
                     /gene="znuD"
                     /locus_tag="ACFNMK_000420"
                     /gene_synonym="tdfJ"
     CDS             2227..4503
                     /gene="znuD"
                     /locus_tag="ACFNMK_000420"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC6363751.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETDRSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLHQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHAHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(5623..6420)
                     /locus_tag="ACFNMK_000421"
     CDS             complement(5623..6420)
                     /locus_tag="ACFNMK_000421"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC6363752.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSICCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVKTVLARLPLTAP
                     QIESEPESEPALQTASVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            6575..9433
                     /gene="uvrA"
                     /locus_tag="ACFNMK_000422"
     CDS             6575..9433
                     /gene="uvrA"
                     /locus_tag="ACFNMK_000422"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC6363753.1"
                     /translation="MCNHRPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPGRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGHLKGKGNSIVIIEHNLDVIKTADYIVDLGP
                     EGGDGGGKIIASGSPEEVAKVKGSYTGKYLKSILLINM"
     gene            9460..10524
                     /locus_tag="ACFNMK_000423"
     CDS             9460..10524
                     /locus_tag="ACFNMK_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFC6363754.1"
                     /translation="MIDNKLKKLEEEQMFESDVNNIPPSDIVAFNELRSCADLLRLHS
                     SGQLNIQPDFQRDIVWSTLDQTRFIDSLTKQLPIPSLCISYDYKTDKRLVIDGLQRLN
                     SIINFLSDDEWKLSKLEDIDQRLAGKTVYTIKTREKELFERVQNVVIPITVIRCDYSK
                     KEHNQYLFTIFHRLNSGGVRLNNQEIRNCIYNGSFNKLLKELANSEETIRLFKKKKRF
                     ANEEMILRFFSFYYNLDDYNGSLAKFLNDMMFEKIYLDENEAESMKELYLRTVNFIYE
                     KIFDHYSSSNFGKTFLEGLLIGVAKNLDYLKNKENEEIKMLFDNFRNLPEYSIDSLKE
                     GLSNKDKVKNRIRASINCFS"
     gene            10543..11037
                     /locus_tag="ACFNMK_000424"
     CDS             10543..11037
                     /locus_tag="ACFNMK_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease"
                     /protein_id="HFC6363755.1"
                     /translation="MDKILIEQTLNNLDSLYQNNPADQVYYSKLAILELSGWLEETID
                     ELLIHYSQSKLNSDNLKYFEEKIKNTWGANYSSNIRNLLIIVVGLINVETIENNLKQN
                     GGYLDLLKNTTKSLKKARDDCAHTSTAGVTQQFQAPSYYKQVLINIYDSLIELERELN
                     LINP"
     gene            11462..11791
                     /locus_tag="ACFNMK_000425"
     CDS             11462..11791
                     /locus_tag="ACFNMK_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6363756.1"
                     /translation="MDVQKQYSFETVAVCVLPNHIHAIWTLPPDDADYSLRWRLIKTK
                     FSAHFPHAENLSASKQRRHERGIWQRCFYEHTVRDETDLQHRADYIHSNPIKQRMSRA
                     KTESRKK"
     gene            complement(12028..12918)
                     /gene="sucD"
                     /locus_tag="ACFNMK_000426"
     CDS             complement(12028..12918)
                     /gene="sucD"
                     /locus_tag="ACFNMK_000426"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC6363757.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPDECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKTKGLA"
     gene            complement(12929..14140)
                     /gene="sucC"
                     /locus_tag="ACFNMK_000427"
     CDS             complement(12929..14140)
                     /gene="sucC"
                     /locus_tag="ACFNMK_000427"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC6363758.1"
                     /translation="MEANSGHSTNLKEKSMNLHEYQAKELLASYGLPVQGGILAHNGE
                     EAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTD
                     ANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKI
                     FKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLA
                     VRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIG
                     CMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINI
                     FGGIVRCDMIAEAIVAAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLND
                     AAEKIVAAVNA"
     gene            complement(14194..14481)
                     /locus_tag="ACFNMK_000428"
     CDS             complement(14194..14481)
                     /locus_tag="ACFNMK_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363759.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(14606..16039)
                     /gene="lpdA"
                     /locus_tag="ACFNMK_000429"
     CDS             complement(14606..16039)
                     /gene="lpdA"
                     /locus_tag="ACFNMK_000429"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6363760.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADTKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            16236..16349
                     /locus_tag="ACFNMK_000430"
                     /pseudo
     CDS             16236..16349
                     /locus_tag="ACFNMK_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(16404..17600)
                     /gene="odhB"
                     /locus_tag="ACFNMK_000431"
     CDS             complement(16404..17600)
                     /gene="odhB"
                     /locus_tag="ACFNMK_000431"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC6363761.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAAPA
                     EAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAA
                     AAPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFE
                     KEHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPI
                     LRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPP
                     QSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPA
                     RLLLDL"
     gene            complement(17700..20528)
                     /locus_tag="ACFNMK_000432"
     CDS             complement(17700..20528)
                     /locus_tag="ACFNMK_000432"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC6363762.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDTVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHLGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNARSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(20733..22016)
                     /gene="gltA"
                     /locus_tag="ACFNMK_000433"
     CDS             complement(20733..22016)
                     /gene="gltA"
                     /locus_tag="ACFNMK_000433"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC6363763.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(22128..22376)
                     /locus_tag="ACFNMK_000434"
     CDS             complement(22128..22376)
                     /locus_tag="ACFNMK_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC6363764.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(22380..23087)
                     /locus_tag="ACFNMK_000435"
     CDS             complement(22380..23087)
                     /locus_tag="ACFNMK_000435"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC6363765.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCIDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(23207..24970)
                     /gene="sdhA"
                     /locus_tag="ACFNMK_000436"
     CDS             complement(23207..24970)
                     /gene="sdhA"
                     /locus_tag="ACFNMK_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC6363766.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(24973..25314)
                     /gene="sdhD"
                     /locus_tag="ACFNMK_000437"
     CDS             complement(24973..25314)
                     /gene="sdhD"
                     /locus_tag="ACFNMK_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC6363767.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFALPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(25308..25685)
                     /gene="sdhC"
                     /locus_tag="ACFNMK_000438"
     CDS             complement(25308..25685)
                     /gene="sdhC"
                     /locus_tag="ACFNMK_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC6363768.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(25964..27367)
                     /locus_tag="ACFNMK_000439"
     CDS             complement(25964..27367)
                     /locus_tag="ACFNMK_000439"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6363769.1"
                     /translation="MKKIQADVVVLGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEAKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(27616..28353)
                     /locus_tag="ACFNMK_000440"
     CDS             complement(27616..28353)
                     /locus_tag="ACFNMK_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC6363770.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(28630..30906)
                     /gene="metE"
                     /locus_tag="ACFNMK_000441"
     CDS             complement(28630..30906)
                     /gene="metE"
                     /locus_tag="ACFNMK_000441"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC6363771.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHEAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTIVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWV
                     EAYKDVYATLNKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSTGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(31042..31920)
                     /gene="metF"
                     /locus_tag="ACFNMK_000442"
     CDS             complement(31042..31920)
                     /gene="metF"
                     /locus_tag="ACFNMK_000442"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC6363772.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            32120..32395
                     /locus_tag="ACFNMK_000443"
     CDS             32120..32395
                     /locus_tag="ACFNMK_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC6363773.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            32395..32520
                     /gene="ykgO"
                     /locus_tag="ACFNMK_000444"
     CDS             32395..32520
                     /gene="ykgO"
                     /locus_tag="ACFNMK_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC6363774.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
BASE COUNT         8263 a         8364 c         7725 g         8190 t
ORIGIN      
        1 acagccccaa gtttttatat aaaaacagtt tggtaagttc tcttcgtgaa gtgcttatgt
       61 tttgccacaa aagttccata atcatggcgg catcacccaa gctccatctt tccaagaaaa
      121 tggcatcttt gttgtggttg aaaaccgaca aactcctctt ttttcgattt ggccctgcct
      181 gaatcgatgg cttcccgtgc ggcagatatg ccgtctgaaa gcgaagcagc gacatttccg
      241 gcatacaggg cggcggcggt gttgagcaat acgatatcgc gcgcagcccc ttctcttcct
      301 tccagcacct cattcatttt caacaaagat tcctgagtat tggcaacttt gatttcatcc
      361 aaattgcggc gggtttcgat accgaaatct tctgggcgga tgtcgtattc gctgattttt
      421 ccgtctttga gctcggcaac gcgtgttttg cccgtcagtg taatttcatc caaaccgccc
      481 tccccgcaaa caaccaaaac gtgttttgaa ccaagttgtt gcaagacccg cgacaaaatg
      541 ccacacaaat cggtgtggaa cacgcccaaa agctggttcg gcgcgcccgc aggattcgtt
      601 aacggaccca atatgttgaa aatacttcgg aaaccgagcg aacggcgtac aggggcgaca
      661 tggcgcatgg cactgtggtg attgggcgcg aacataaacc cgatgccggt ctgcctgata
      721 ctttgggcaa cctgttcggg agtcaggttg aggtttgcgc ccatctgctc caccacgtca
      781 gccgcaccgc tggaggaaga gaccgaccgg cctccgtgtt tggcaacctt cgcgcctgcc
      841 gctgcggcaa caaacatcga agtcgtcgaa atattgaagg ttttcgcgcc atccccgccc
      901 gtaccgacga tatcgaccag cccctctgca ttctccagcg gcacttttgt cgcaaactcg
      961 cgcatgacgg ctgcagctgc ggtaatttcg gaaacggttt caaccttgat acgcaatcct
     1021 gtcaaaatcg ccgcaatttg ttcgggcggc acttttccgc tcatcatttg gcgcatcaag
     1081 tcggtcattt catcgtaaaa caactcatta ttgctgatta atcgttcaat agcctgttgc
     1141 ggtgtaatca ttttttgtcc tccgttcaat attcggacga aaatgccgtc tgaagggctt
     1201 cagacggcat cgcgtcagat tttttgcggt ttgaagtttt gaaattcgat taaaaaattg
     1261 ttcaacatat catgtccgtg ttcggtcagc agagcttcag ggtggaattg taccccctca
     1321 acagcatatt ccttatggcg cacacccata atctcgccgt cctcagtcca agccgttact
     1381 tccaaacatt cgggcatcgt attccgatcg ataacgaggc tgtgataacg cgtacaggta
     1441 accggattgg gcaaaccttt aaacataccc ttgcccgaat gggacacggg cgacacctta
     1501 ccgtgcatca gcgttttggc gcggactatc ctgccgccga acgcctcgcc tatcgtctga
     1561 tgcccgaggc acacgcccat aatcggcagc cggccggcaa aatggcgcat cgccgccacg
     1621 gaaatccccg cttctttggg cgaacacgga ccgggtccga taacgagata ttgcggattc
     1681 aatgcctcga tttcttccaa cgtaatatca tcgttgcggc gcacggcgac ttcctgcccc
     1741 aattcagcga aatactggac gatgttgtaa gtaaaactgt cgtaattgtc gataaacaaa
     1801 agcattttgt gattaaccta ttgatttata atttgtttgt tattaactgc atttccgata
     1861 cccccatcca taccctctta tatcttagcg tgcccgatgc gccctcgtga acctagcgca
     1921 gagcctcgcg ttctgacaag tacgccattt tgccaccttt tcgaccgttc gggaacgtac
     1981 cgtttttatg cactcataaa ttttggttat cccgcaaccg aacatagtgg gcaaacatag
     2041 cggaatctgc gtcaacatca accgcgcccc cgaatgacgg cggatacaaa tggacaccgc
     2101 ccgatgccgc aaccgtttca tttgaaaaac ttttgcctat gccctctgag tgttctcaaa
     2161 atcttatgat ataaaaaatg taatgttata taataacaaa cttttgaaat acgacgggaa
     2221 gccaaaatgg cacaaaccac actcaaacct attgttttat caattctttt aatcaacaca
     2281 cccctcctcg cccaagcgca tgaaaccgat cggtcggtgg atttggaaac ggtcagcgtc
     2341 gtcggcaaaa gccgtccgcg cgcgacttcg gggctgctgc acacttctac cgcctccgac
     2401 aaaatcatct ccggcgatac cttgcaccaa aaagctgtca acttgggcga tgctttagac
     2461 ggcgtacctg gcatccacgc ttcgcaatac ggcggcggcg cgtccgctcc cgttattcgc
     2521 ggtcaaacag gcaggcggat taaggttttg aaccatcacg gcgaaacagg cgatatggcg
     2581 gatttttcgc ccgatcacgc cattatggta gataccgcct tgtcgcaaca ggttgaaatc
     2641 ctgcgcggtc cggttacgct cttgtacagc tcaggcaatg tagccggttt ggtcgatgtt
     2701 gccgacggca aaatccccga aaaaatgcct gaaaacggcg tatcgggcga actcggattg
     2761 cgtttgagca gcggcaatct ggaaaaactc acgtccggcg gcatcaatat cggtttgggc
     2821 aaaaactttg tattgcacac ggaaggcttg taccgcaaat cgggcgatta cgccgtaccg
     2881 cgttaccgca atctgaaacg cctgcccgac agccacgccg attcgcaaac gggcagcatc
     2941 gggctgtctt gggttggcga aaaaggcttt atcggcgcag catacagcga ccgtcgcgac
     3001 caatatggtc tgcctgccca cagccacgaa tacgatgatt gccacgccga catcatctgg
     3061 caaaagagtt tgattaacaa acgctatttg cagctttatc cgcacctgtt gaccgaagaa
     3121 gacatcgatt acgacaatcc gggcttgagc tgcggctttc acgacgacga tgatgcacac
     3181 gcccatgccc acaacggcaa accttggata gacctgcgca acaaacgcta cgaactccgc
     3241 gccgaatgga agcaaccgtt ccccggtttt gaagccctgc gcgtacacct gaaccgcaac
     3301 gactaccgcc acgacgaaaa agcaggcgat gcagtagaaa acttttttaa caaccaaacg
     3361 caaaacgccc gtatcgagtt gcgccaccaa cccataggcc gtctgaaagg cagctggggc
     3421 gtgcaatatt tacaacaaaa atccagtgct ttatctgcca catccgaagc ggtcaaacaa
     3481 ccgatgctgc ttgacaataa agtgcaacat tacagctttt tcggtgtaga acaggcaaac
     3541 tgggacaact tcacgcttga aggcggcgta cgcgtggaaa aacaaaaagc ctccatccgc
     3601 tacgacaaag cattgattga tcgggaaaac tactacaacc atcccctgcc cgacctcggc
     3661 gcgcaccgcc aaaccgcccg ctcattcgca ctttcgggca actggtattt cacgccacaa
     3721 cacaaactca gcctgaccgc ctcccatcag gaacgcctgc cgtcaacgca agagctgtac
     3781 gcacacggca aacacgtcgc caccaacacc tttgaagtcg gcaacaaaca cctcaacaaa
     3841 gagcgttcca acaatatcga actcgcgctg ggctacgaag gcgaccgctg gcaatacaat
     3901 ctggcactct accgcaaccg cttcggcaac tacatttacg cccaaacctt aaacgacgga
     3961 cgcggcccca aatccatcga agacgacagc gaaatgaagc tcgtgcgcta caaccaatcc
     4021 ggtgcggact tctacggcgc ggaaggcgaa atctacttca aaccgacacc gcgctaccgc
     4081 atcggcgttt ccggcgacta tgtacgaggc cgtctgaaaa acctgccgtc cctacccggc
     4141 agggaagatg cctacggcaa ccgtcctttc atcgcgcagg acgaccaaaa cgcccctcgc
     4201 gttccggctg cgcgcctcgg cttccacctg aaagcctcgc tgaccgaccg catcgatgcc
     4261 aatttggact actaccgcgt gtttgcccaa aacaaactcg cccgctacga aacgcgcacg
     4321 cccggacacc atatgctcaa cctcggcgca aactaccgcc gcaatacgcg ctatggcgag
     4381 tggaattggt acgtcaaagc cgacaacctg ctcaaccaat ccgtttacgc ccacagcagc
     4441 ttcctctctg atacgccgca aatgggtcgc agctttaccg gcggcgtaaa cgtgaagttt
     4501 taaaatcgga caggcaaaca aaaaaggttt tcagacggca ttctgaaaac cttttttctt
     4561 tttaatcaat ttgttaggaa atatggcaaa gacaaagaac aaaccactat gccatcattc
     4621 ccattttctt tgtttcatca ttcttgcttt cacgccgtca ttctcgcaaa agcagaaaaa
     4681 tcaaaagcag aaacccatca ttcccgcgca ggcgagaatc caattcattc ggtttcagcc
     4741 gtttccgata aatcgcctta gcatttgggt tctagattcc cactttcgtg ggaatgacgc
     4801 ggaaaggttt ctgttttttt taagttttag gtaactaatg tgccatcatt cccgcgcagg
     4861 cgggaatcca attcgttcgg tttcagccgt ttccgataaa tcgccttagc atttgggttt
     4921 tagattccca ctttcgtggg aatgacggcg taaaggtttc tgttttttaa gttttaggta
     4981 actaataagt cgtcattccc gcgcaggcgg gaatccaatt cgttcggttt ctgttatttc
     5041 cgataaatca ctgtaacgtt aagttcccgg attcccactt tcgtgggaat gacggcgtaa
     5101 aggtttctgt tttttaagtt ttaggtaact aataagtcgt cattcccgcg caggcgggaa
     5161 tccaattcgt tcggtttcag ccgtttccga taaatcgcct tagcatttgg gttctagatt
     5221 cccactttcg tgggaatgac ggcgtaaagg tttctgtttt ttaagtttta ggtaactaat
     5281 aagtcgtcat tcccgcgcag gcgggaatcc ggtctcttcg gtttcagccg tttccgataa
     5341 atcgccttag catttgggtt ctagattccc actttcgtgg gaatgacggc gtaaaggttt
     5401 ctgtttttta agttttaagt aactaatgag ttgtcattcc cgcgcaggcg ggaatccagt
     5461 ccgtccggtt tcagtcgttt ccgataaatc gtcgtaacgt taagtttcta gattcccact
     5521 ttcgtgggaa tgacggtgtg aacattagca aaaattcaaa aaaccaaact tcctcattcg
     5581 tgtgaatgat ggtactgata ttttctattt cagttcgcaa aattaatctt tcaacacatc
     5641 ttgcacagcc gcctgaatct tagccgccgc tgcttcaatt tgccgttgtt cggcagatgg
     5701 gtttgccgct gtttcaaccg aagcagtttg taaagctggc tcagattcag gctcggattc
     5761 gatttgcggc gcagtcagcg gcagacgcgc caatacagtt tttacgccgg ttactttatc
     5821 gccaatcgcc acttgcgcct gcgcatcgac aggcagatac atatccacgc gcgaaccgaa
     5881 gcggataaag ccataacgtt cgccgcggga cagtttcgca cctgcttggg tgtagcacaa
     5941 aatacggcgc gccaccaaac cggccacttg aacaaaagta atttcacgac cggaagccgt
     6001 agtcgccaac accgcattac gttcgttttc cgtgctggct ttgtccaaat ccgcattcac
     6061 gaatttgcct ttgttataga ccactttcgt taccgtacaa tcggcaggcg atttttgcga
     6121 atgcacgttg aacacgttca taaaaatact gattttcaaa gcatcgacat cacgatacgg
     6181 atcgcgtgcg cgctcgacca ccacgatacg gccgtcaaca ggactcaaca ccgcttcagg
     6241 attttgcgga atctcacgcg cagggtcgcg gaaaaactgc aatgcaaata cggtaaacac
     6301 ccaaaacggc aaagaccacc agccgcaaca tatcgacacc agcaggctca aagccaaacc
     6361 gccgccaata atcggccagc cctcacgggc gataatcggg tgggggtaaa gacggttcat
     6421 ttgtcatcct tttgttcaag cgagttaaaa ttgccgccat tatagcggaa tccgccgcgt
     6481 tccgccgaca aatgccgtct gaacgcggct tcagacggca tccgagaaag tgctacaata
     6541 tcggttttcc tttatttttc aggcggcatc cattatgtgc aaccatcgtc cccaacattc
     6601 gcacgacaac gacaccatcc gcatacgcgg cgcgcgcacg cataatttga aaaacatcga
     6661 tttggacatt ccgcgccaca agctggtggt ggtaacagga ttgtcgggca gcggcaaatc
     6721 gtcgctggca tttgatactt tatatgccga aggtcaacgc cgttatgtcg agagcctttc
     6781 cgcctatgcg cggcagtttt tgcagatgat ggacaaaccc gatgtcgatt tgattgaagg
     6841 tctgtcgccc gcgatttcca tcgagcaaaa atccaccagc cacaatccgc gttccaccgt
     6901 cggcacggta acggaaatcc acgactatct gcgccttttg tacgcccgcg tcggtacgcc
     6961 gtattgcccc gaacacaatc tgccgctatc cagccaaacc gtatcgcaga tggtcgatgc
     7021 cgtattgaaa ctgccggaag acacgcgcgt gatgattctg gcgccggcgg tacgcgagcg
     7081 taagggcgag tttgtcgatt tctttgccga cttgcaggcg cagggttttg cgcgcgtgcg
     7141 cgtggacggc gaggtctatc agcttgacga agtgccgaag ctggaaaaaa acatcaagca
     7201 caacatcgac gtggtcatcg accgcgtgaa agtgaaggcg gacatcaagc agcggctggc
     7261 ggaaagtttt gaaaccgcgt tacgccacgg caacgagcgc gcgctggcga tggagatgga
     7321 cagcggcgaa gaacattggt tttccgcgcg tttcgcctgc cccgtatgtt cgtacagcct
     7381 gccagaatta gagccgcgcc tgttttcctt caacaacccg atgggttcct gcccgacttg
     7441 cgacggcttg ggcaacacca atttcttcga ccccgaaaaa gtggtcgccc accccgaatt
     7501 gtcgctggct tcgggcgcga ttgacggctg ggacaaacgc aaccagttct atttccaaat
     7561 gattcagtcg ctggcacggc attacggttt tgatgtgcag gctgcttggg aaaccctacc
     7621 tgcaaaaatc aaaaaagtcg tgttgcacgg ttcgggcaag gaagtcattg atttcaccta
     7681 cctatccgaa cgcggcacca ccttcaaccg cagccacgcc ttcgaaggca tcattcccaa
     7741 tctcgaacgc cgctaccgcg aaaccgacag cgaaaccgtg cgcgaaaaat tgcgcgaata
     7801 ccaaaaccac cgcgcctgcc cgagctgcgg cggtgcacgt ttgcgcaaag aagcgcgcta
     7861 cgtttacgtc agcggcgaac cgttgcacga agtctccgcc tggccgctga ccaaaaccca
     7921 ccgattcttt gaaacgctgg atttggacgg caacaaaaaa caaatcgccg aaaaaatcct
     7981 caaagaaatc accgagcggc tcggcttttt gattaacgtc gggctggatt acctgaatct
     8041 ctcccgctcc gccgaaaccc tttccggcgg cgaagcccag cgcatccgac tcgccagcca
     8101 aatcggcagc ggcctgaccg gcgtgatgta cgttttggac gaaccctcca tcggcctgca
     8161 ccagcgcgac aacgaccgcc tgctcgccac cctcaaacgc ctgcgcgatt taggcaacag
     8221 cgtgattgta gtcgaacacg acgaagacgc catccgcgaa gccgatttcg tcgtcgatat
     8281 gggcccgggt gcgggtgagc acggcggcaa cgtactgatt gccgataccc ccgaaaacgt
     8341 cgccaaatgc gaaaactccg ttaccggaca atacctcagc ggcaaaaaat ccattgccgt
     8401 gccgtctgaa cgcacgcccg tcaatcccgg ccgaatgctc gtcctcaaag gcgcgcgcgg
     8461 caacaacctc aaaaacgtta ccctcgaact gccgctcggt ttaattacct gcatcaccgg
     8521 cgtatccggc agcggcaagt ccaccctgat taacgacacc ctcgccaaaa tcaccgcccg
     8581 cgaactcaac cgcgcccaag aagaacccgc cccatacgac gacatccgcg gcctcgaaca
     8641 cctcgacaaa gtcatcaacg tcgaccaatc ccccatcggt cgcaccccgc gctccaaccc
     8701 cgccacctac accggcctgt tcacccccat ccgcgaactc ttcgccggcg tacccctctc
     8761 gcgcgaacgc ggctacaacg tcggcagatt ctccttcaac gtcaaaggcg gccgctgcga
     8821 agcctgccaa ggcgacggcg tgattaaagt cgaaatgcac ttcctgcccg acgtgtacgt
     8881 cccctgcgaa gtctgccacg gcaaacgcta caaccgcgaa accctcgaaa tccaatacaa
     8941 aggcaaaaac atcagtcaag tcctcgacat gaccgtcgaa gaagcccgcg aattcttcga
     9001 cgccgtcccc accgtatcgc gcaaactgca aaccttgatg gacgtaggct taggctacat
     9061 ccgtctcggc caatccgcca ccaccctctc cggcggcgaa gcccagcgcg tcaaacttgc
     9121 tttggaactc tccaaacgcg acaccggcag aacactctac atcctcgacg aacccaccac
     9181 cggcctgcac ttcgccgaca tcgccctgct gctggaagtc ataggtcatc tgaaaggcaa
     9241 aggcaactcg attgtgatta tcgagcataa tcttgacgtt ataaaaacgg ctgattatat
     9301 tgtggatttg gggccggaag ggggagatgg aggtggtaaa attattgctt caggtagccc
     9361 agaagaagta gcgaaagtta aggggagtta tactgggaag tatttgaaaa gtattctcct
     9421 aataaatatg taaatcttaa attttatgga gtatctaaaa tgattgataa caaattaaaa
     9481 aaacttgaag aagaacaaat gtttgaaagt gatgtaaata atatccctcc atctgatatt
     9541 gttgctttca atgaactaag atcatgtgca gatttattac gtttgcattc ttcaggccaa
     9601 ttaaatattc agcctgattt ccagagagat atcgtttggt ccactctaga ccaaacaagg
     9661 tttattgaca gtttaacgaa gcaacttcca attcctagtt tatgtattag ttacgattat
     9721 aaaacagata aacggttagt tatagatggc ttacaacgtc taaacagtat tatcaatttt
     9781 ttgtctgatg atgaatggaa actttcaaag cttgaggata ttgatcaaag acttgctgga
     9841 aaaactgttt atacaatcaa aactagggaa aaagaattat ttgaacgcgt acaaaatgta
     9901 gtaattccta taacagtgat cagatgtgat tactcaaaaa aagagcacaa tcaatattta
     9961 tttactattt ttcatcgact gaattcaggt ggagttagat taaataatca agaaataaga
    10021 aattgtattt ataatggatc attcaataaa cttcttaaag aattggctaa ttctgaagaa
    10081 acaataagat tatttaaaaa gaaaaaacgg tttgctaatg aagaaatgat attaagattt
    10141 ttctcctttt actataattt agatgattac aatgggagtt tggctaagtt tctaaatgat
    10201 atgatgtttg aaaaaattta tttagatgaa aatgaggctg aatctatgaa agaattatat
    10261 ttacgcacag tcaattttat ttatgaaaaa atttttgatc actattcatc atctaatttt
    10321 ggaaaaacat ttcttgaagg tttattgatt ggtgttgcaa aaaatttaga ttatttaaaa
    10381 aataaagaaa atgaagaaat taaaatgcta tttgataatt ttagaaactt accagaatat
    10441 agcattgata gtctaaagga gggcttatct aataaggaca aagtaaaaaa taggatacgt
    10501 gcatctatta attgtttttc ttaagcaaca ctaggtataa acatggataa aattttaata
    10561 gaacagacac ttaataactt agatagcctt tatcagaata atccagctga tcaagtttat
    10621 tattcaaagt tagcaatatt ggagttaagt gggtggttag aggagactat tgatgaactt
    10681 ttgatccatt attcacagag taaactaaat tcagataatc tcaagtattt cgaagaaaaa
    10741 ataaaaaata cgtggggagc aaattacagt tctaacataa gaaatctttt aattattgtt
    10801 gtaggcttaa ttaacgtaga gactattgag aataatttaa aacaaaatgg aggttatcta
    10861 gacttactga aaaatactac taaatcctta aaaaaagctc gagatgattg tgcgcacact
    10921 tccacagctg gagtaacaca acaatttcaa gcaccatctt attataaaca agttctaatt
    10981 aatatatacg attcattgat agaattagaa agggaattaa atcttatcaa cccgtgaatc
    11041 acacgaaact tcgaaaccaa cgtgtagcaa gccgtagcct gcatactcaa ttaaccgcgt
    11101 gcgtgactac gctacacacc ctgcctatga atcaaacgag acttcaaaac caacgtgtag
    11161 ggtatatgcg gtacgcacgc ggttgctgct tgattgacgg tttgccgttt caatttttaa
    11221 cttcgttgaa gctagcgctt tcagacgacc tccgatatcg tctgaaaacg tcaaatttaa
    11281 caaagctaaa acctaaaaac aacaaaccat tccttctcca acacccaaac cacaaacaaa
    11341 aatcctatgt cgcgttatcg cagaaacttc attgccggcg gcacattctg tttcactgtc
    11401 aaaccctccg ccccgaaatc gcgcctgctt gtcgaacata tcgacttcgc gggcagctta
    11461 tatggatgtg caaaaacaat attcctttga aaccgtcgcc gtatgcgtgc tgccgaacca
    11521 tatccacgcc atttggacgc tgccacccga cgatgcggat tattccctgc gctggcggct
    11581 gattaaaacc aaattctccg cacatttccc tcatgccgaa aacctgtctg ccagcaaaca
    11641 gcggcggcac gaacgcggta tttggcaacg gtgtttttac gaacacaccg tgcgcgatga
    11701 aacggatttg cagcatcgtg cggactacat ccattccaat cctatcaaac aaagaatgtc
    11761 gcgtgctaaa acagaatccc gcaaaaaata atattagtct tttcaaatgc gatacttatc
    11821 cattcccgga cttcaaacaa ggcgtgtgca acctgactta cgggtttgct cctgtttgac
    11881 cgataaatat tctgactgcc tcaaagggca aaaaataatg tgggtatgtt gaggctgcct
    11941 ctttttgaaa tgacaaacaa aatgccgtct gaaacctgat ttccggtttc agacggcatt
    12001 tgatgttcaa tcagttgtca aacctgatta tgccaaacct tttgttttca acacttccag
    12061 catggtagtg cccaactcgg cagggctgcg ggtgtaagcg ataccggctt tttcgaaagc
    12121 ggcgaatttt tcctcggcag tacctttacc gccggaaata atcgcaccgg cgtggcccat
    12181 acgtttgcct ttaggagcgg taacaccagc aatgtaacct acaacaggtt tggttacgtt
    12241 ggattggatg tattcggctg cttcttcttc cgcagtaccg ccgatttcac cgatcatgat
    12301 gatggcgtcg gtatccgggt cttcttggaa gagtttcagt gcgtcgattt gattcatacc
    12361 aggaatcggg tcgccgccga taccgataca ggttgattga cccaagccca gtttggtggt
    12421 ttgtgccacg gcttcgtaag tcaatgtacc ggaacgggaa ataataccga tgcggcccgg
    12481 agtgtggatg tggcccggca taataccgat tttgcactca tccggagtaa tcacgcccgg
    12541 gcagttaggg ccgaccaagc gggtaccgtt gccgttggtt tccaaataac gtttggcttt
    12601 gagcatgtcc agagtaggca cgccttcggt aatcacaacg accaagccta cgcctgaatc
    12661 aacggcttca acgatagaat ccaacacaaa cggagcggga acgtaaatta cagatgcatc
    12721 cgcgccggtt tctttaaccg cttctttcat ggtgttgaat acaggcaggt tcaggtgggt
    12781 ttgaccgcct ttgcccgggg taacgccgcc gacaacttta gtgccgtaag ccagagcttg
    12841 ttcggagtgg aaagtaccgt ttttaccggt gaaaccttga accaatactt tggtgtcttt
    12901 attaatcaat acgctcattc ttttctcctt aggcgtttac ggctgcaaca attttttcgg
    12961 ctgcgtcatt caggccgtct gcagaagtca gtttcaggcc tgattcgttc aggattttcg
    13021 cgccgagttc ggcgttgtta ccttccaaac gaacaacgac aggaacgtcg acgttgattt
    13081 ctttaacggc tgccacgatg gcttccgcaa tcatgtcgca acgtacgata ccgccgaaga
    13141 tgttgatcaa tacgccttta acggatttgt cttccaaaat cagtttgaac gcttcaacca
    13201 cgcggtcttt ggttgcgccg ccgccaacgt ccaagaagtt ggcaggttgg ccgcctttca
    13261 gtttgatgat gtccatggtg gccatcgcca aaccggcacc gttaaccata cagccgatat
    13321 taccttctag ggcaacatag ttcaggtcga attcagaagc tttcagttca cgttcgtttt
    13381 cttgagattt gtcgcgcaat tcagcgattt ttggcaggcg gtagagcgcg ttgctgtcga
    13441 tgccgatttt gccgtccacg caagccagtg cgccgttttc gcgaactgcc agcgggttga
    13501 cttcaaacag ggcgaagtcg ttttcgacaa acgctttgta cgcgccggtc atcagtttga
    13561 cgaactcgtt gatttgtttg tctttcaagc ccagttggaa ggcaacttcg cgagcttggc
    13621 aaggttgcag gccgaccagc ggatcaacgg ttactttgaa gattttttct ggagtttcgg
    13681 cagcaacttt ttcgatttcc acaccgcctt cggtagaagc catgaatgta acgcggcggg
    13741 tagaacggtc aaccactgcg cccaagtaca gctcggtttg aaccggatac atatcttcgc
    13801 aaaccaaaac gctgttgaca ggttgaccgt tggcatcggt ttggtaagtt accaagttgg
    13861 tgccaatcag gctttcagcc acttctttag cttcttcgcg gcttttaacg acttttacgc
    13921 cgcccgcttt accgcggccg ccggcatgta cttgtgcttt gacaacagcg aatttgccgc
    13981 ccaatttgtc gtaagctgca gcggcttctt cgccgttgtg tgccaaaata ccgccttgta
    14041 cgggcaaacc gtagctagcc agcagttctt tagcctgata ctcgtgtaaa ttcatggatt
    14101 tctcctttag attagtggaa tgccccgagt ttgcctccaa atggcaagcc cgtgcgtttc
    14161 agactacctt tcggcggtcg gaaaatcgtc gtttcacttg ctgaaaggca accacatccg
    14221 cgccagcaga tacatggctg caccgcctcc cccgagaata aaaaagacga tgcctttttt
    14281 acgcgaattc ggacagagca gataaacggc aaggctgaaa ctgataaaca acaatgccac
    14341 gcgccaaacc aattctttat ctttgaaacg gttgagcgta taagtttcgg tcatcatcac
    14401 atacatctgc cacaatgcgg ccatacacat gctgatgatg cccatcggta taaagaaaat
    14461 accgactatt tctttgttca taacgaatgt aaatgctgtt tgtttgtctg acggaattat
    14521 aaatcagaaa acggagctct cggaacattc aatttgtcga aggcattaag gtcgtctgaa
    14581 aaatttcaga cggccttaat gtctatcaac cgtgcaaagc gcgtttgtcg gccgccaatg
    14641 cagcttcgtg aaccacttcg gacaaggttg ggtgggcatg gataatgcgg gcgatgtctt
    14701 cgctgctggc gaagaattcg agcgcagtca cgccttcggt aaccaattcg ctgacaaccg
    14761 ggccaatcat gtgtacgccc aagatgcggt cggttttggt atctgccaat actttaaccg
    14821 tacctttagc tttgcccatt gccaatgcgc gaccgtttgc accgaaacct gaagtacctt
    14881 ttttgtactc cacgccttcg gctttgagct gctcttcggt tttacccacc caagcgattt
    14941 cagggtcggt gtaaatcacg aacggtacgt tgttgaagtc gatatgcggt ttttgaccgg
    15001 caatgcgctc ggcaacggca acgccttcgt cgctggcttt gtgtgccaac atcgggccgc
    15061 gaaccacgtc gccgattgcc catacgttag gcaggttggt acggcattcg ccgtccactt
    15121 tgataaagcc gcgctcgtct ttttccaagc ctacggcttc cgcgttcagg cctttggtgt
    15181 ttggaatacg gccgatggca acgatcagtt tgtcgaatac ttcggttttg gcttcgccgg
    15241 cagcagtttc gtaagcaacg gaaacgcctt tgccttcaga tttaatgtca ccgattttta
    15301 cacccaattc gatgctcaag ccttgctctt tggtgaagta tttgaaggct tctttggcga
    15361 tttgttggtc ggcggcagcc aagaaggtcg gcgcagcttc aagaatggta acttctgcac
    15421 ccacgcggtt ccatacggaa cccatttcca aaccaatcac gccggaaccg atcacgccga
    15481 gtttggcagg tacttcggtc aggttcaatg cgccttcgtt gtccaatacg ttcacgttat
    15541 cgatagcgac ttgtggcagc ggacgcggta cggaaccggt ggctacgatg acgtgtttgg
    15601 cttcgataac ggttttctcg cctttgttat cgacttcgat ttggtaagca tcgccatttt
    15661 taccagcaaa ggaagccgta ccgaacagac tggttacttt gtttttttgg aacaggaatt
    15721 tcacgccgcc ggtcagtttg gtcacgatgg catctttgcg ctcaatcatt ttggccgcgt
    15781 cgaattttac gtcgccgaca gtgataccgt gttcggcaaa ctcgtgttgc gctgcgtgga
    15841 aatgttcgct ggattgcaac agggctttag aagggataca gcctacgttc aagcaagtac
    15901 cgcccaatgc aggggcattg cctgctttgt taacgcctgc atcgacacag gcagttttga
    15961 aacccagttg tgcggcacgg atggcggcaa cgtatccgcc cgggcctgca ccaatcacta
    16021 ctacatcata ttgagacatc gtgtcatcct ttgcttttag cagtatctca aaataaaaac
    16081 gatacttttc gtttcttttt tattttgaat gactgcgttt tgatggtttt aagaaaaagc
    16141 cgtctgaaag tccatatctg ccgattcatt cgaatcaaat gtttcagacg gcctcaatcg
    16201 ttcgttgagc ctattgtagc acgatactac attacgctac atctaaatat agtggattaa
    16261 atttaaatca ggacaaggcg acgaagccgc agacagtaca gatagtacgg caaggcgagg
    16321 caacgccgta ctggtttaaa tttaatccac tataaaaatt cagacggcct tttcattaac
    16381 aaataaaagg ccgtctgaaa cgattacaga tccaacaaca ggcgggccgg gtcttccaac
    16441 gcgtctttaa tggctaccaa ggtcaataca gcttcgcgac cgtcgatgat acggtggtcg
    16501 taagacagag ccagatacat cattggacgg acaacaactt gaccgttttc aaccacagcg
    16561 cgctctttag tggcatgcat acccaaaatc gcagattgag gcgggttgat gatcggggta
    16621 gacatcatag aaccgaaagt accgccgttg gtaatactga atgtaccgcc ggtcagatct
    16681 tcgatagcga ttttgccatc tttggctttt ttcgcgtaat caacaattgc ttgttcgatg
    16741 tcggcaatgc tcatttggtc ggcatcgcgc aggattggca caaccaaacc gcgtgggctg
    16801 ccaatcgcga taccgatgtc gaagtagccg tggtacacga tgtctttgcc gtcaacagaa
    16861 gcattcacaa ccgggtattt tttcagggcg gcgacagcgg ctttaacgaa gaaggacata
    16921 aagcccaatt tcacgccgtg ttctttctcg aatttttctt tgtacttcgc acgcaagtcc
    16981 atgattggtt tcatgttgac ttcgttgaat gtagtcagaa tggcgttttc ttgttgagaa
    17041 gccaagaggc gttctgcaac acgggcacgc aggcggctca ttggtacgcg ttcttcagga
    17101 cgtgcgccgg caggggctgc ggctgcggca ggtttggcag cggcattttg tacgtcttct
    17161 ttcaatacgc gaccgtcacg gccggaacct tgcaatgcgt tcacgtcaac accgctctct
    17221 gcagccagtt tggcggcggc aggcatagcg gcgttgtttt gtgcagcagc aggagcggcg
    17281 gccggggcag cttcggcagg agcggcggca ggtgcttcag cagcggcagt agcagcggca
    17341 ggtgcttcag cagcggcagt agcagcggta tcgatacgtg ccaaaacttg gtcggcaaca
    17401 acggtttcac cgtcttgcgc tacgatttca accaatacgc cggcttgtgg agaaggtact
    17461 tccaaaacca ctttgtccgt ttcgatatcg atcaggattt cgtcacgggc aactgcttcg
    17521 ccaacttttt tcttccattc caagagcgtg ccttcagata cgctttcaga cagcataggt
    17581 acttttacat caataatcat tttgtgtctc caatggccct ttcagacggc ctgttgtgtg
    17641 tttttcattc tgcatatggg tgttccgcct gagcagcctc agacggaatg ctacttggct
    17701 tacaatgcca aagcgtcttc aaccaattgt ttcaattgag caatgtgttt gcttgagtag
    17761 cccactgcag gcgatgcgct gcttggacga ccggcataag acagtttttg ctcttcgctg
    17821 ataacgtctt cgatgcggtg gcggatttgg tagaacgcgc cttggttttt cggctcttct
    17881 tgtgcccaaa ccacagatct tgcgttcgga tatttcgcca gttcggcttt aacctcgtcg
    17941 tatgggaacg gatacagctg ctcaacgcgg acaatagcga catcatcttc cagtttacgt
    18001 tcggctcgac cggcttccaa gtcatagtaa acctgaccgg cacacaatac cacgcgtttc
    18061 acgctgtcgt tgcttgcgcg ttcggcggta tcgccgataa ctggacggaa agtcgaacct
    18121 tcggtgaagt tttccagcgg gctcattgcc cctttgaatc gcaacaggcg tttggacatg
    18181 aaaattacca gcggtttgcg gtatgaacct aagacttgac gttgcaagag gtggaacatt
    18241 tgcgacgctt cagacggcat gatgacttgc atattgttct cagaacacag ttgcaaccaa
    18301 cgttctacgc gtgcagaaga gtgctcggga ccttgaccgt cgtagccgtg cggaaggatg
    18361 gtggtcagac cacacaaacg accccatttg gtttcgcctg aagacaggaa ttggtcaata
    18421 gtcacttgcg cgccgttggc gaagtcacca aattgagctt cccaaatggt cagcttgtca
    18481 ggcgcggagc aggcaaagcc gtactcgaac gccatcacgg cttcttcgtt caaaatggag
    18541 tcgataacca agaactcgcc cataccttcg cccatatggc gcagaggaac ataagtaccg
    18601 tcatcccatt tttcacgttt ttgatcgtgc aatactgcat gacggtgtga gaatgtaccg
    18661 cgcccggagt cctcaccgga gatacgcacg ccatgacctt tggttacaag gcttgcgtat
    18721 gccaaggttt cggccatacc ccagtcgatg gcttgtttgc cggatgccat ggctttacgc
    18781 gcttcaatca cacgttttgc agtcggatgc agggcaaagc cttccggtac ggcggtaaat
    18841 ttctcggtaa gacgctcaat atccgcggca ggcaaaccgg tttcgatgtg ttcgcgccaa
    18901 tctttacctt ggtatttgct ccagtcaatt tgtgtgcgtt ggaagttgct caacgttgtt
    18961 tgctcgacgt gttcgccttt atccaaagca tcacggtagg cttggatgta accgtcagcc
    19021 tcggcttggg ttaccacgcc ttcggcaatc agttgctcgg tgtacaaagc acgcgcaccc
    19081 aggtgttgcg atactttttt gtacatcatc ggttgggtca aggtcggatc gtcgccctcg
    19141 ttgtggcccc atttacggta gcagacaacg tcgatcacga tgtctttatg gaattttttg
    19201 cggtaatcca aagcggcttg gatggcaaag caaacacgct caggatcatc gccgttcaca
    19261 tggataaccg gagcggaaac catttttgcg atatcggtac agtgtacggt tgaacgggta
    19321 tcgcggatat cggaagtggt aaagccgatt tgattgttga tgacgatatg aaccgtaccg
    19381 ccggtggtat aaccgcgcgt tttagacagg ttgaatgtcg cttggttgac acccaaaccg
    19441 ataaatgcgg agtcgccgtg aatcaatacg ggcaagactt tatcgcggcc gttttcgccc
    19501 aaacgttttt gcttggcgcg tgcagaacct tccactaccg gattgacgat ttccaagtgg
    19561 gacgggttga acgccaaaga aacatgcatc gggccgtgcg gggtagcaat atcggagctg
    19621 aagcccatat ggtatttcac gtcgccgctg ggcagtttga tttcggcacg accttcaaat
    19681 tcggcaaaca aatcgccggg ttttttgccc aaaatgttca ccaaaacatt cagacggcca
    19741 cggtgcgcca taccgatgat gacttcttcc acgccgtctt taccggcgtt ttgaatcagg
    19801 tagttcaaac cggcaatcgc gctttcgccg ccttcgacac cgaaacgttt ctgaccgaca
    19861 tatttggtat gcagataacg ttccaaagtc tcggcagcag tcatctcttt caagatacgg
    19921 cgtttttgat cggcattgta atgcggtgtg gacaatacgc tttcaaaata attgcgtacc
    19981 cagcggcgct cttcggtatt gggaatatag atatattcca atgcgatgtg gccgcagtag
    20041 gtttgtttga ggttgctgat gatttgggac aaaggcagtt tgccgcgatt ggcaaaatcg
    20101 ccctcgccca tattgaattg aagcgccata tcggcatcag acagaccgtg gaatttcgga
    20161 tcgagggctt caatatcgcg cggggggata cgtttgagcg gatcaagttg ggctgcaccc
    20221 acgccttgga tacgataggc agaaatcagc cgtaaaacgc tgacttgctt tttcagcatt
    20281 gcctcatccg caccgcccgc aacggcagat gcaattttct ttttcgccaa agtaacaaat
    20341 gattcgcgaa tcggtgtgtg tgcgacatcg acagcaaccg tccccggctg tttgctcaaa
    20401 tcggtgaaat actgcttcca tttttcatca accgtatcgg ggttttccaa aaaagcctcg
    20461 tacaattcct caatgtaggg tgcgttcgaa ccgaacaggt aagagaaatt gagtttttcg
    20521 tccatcatgg cgtgcgctct tttctttaaa atataaacaa agggtagaag tccgcgtcct
    20581 gacggctaaa ctgatgccgt ctgaaaagac attttattct accctatttt aaaccggatt
    20641 gcttgtcttg atttcatccg actacaatca gacggattcg ggaaacaatc cggttctctg
    20701 atattgcctg tttgacaata tattaatgta tgttacctct cgcctggcgg cacataatcg
    20761 cggcgttccg aaccggtata aagctggcgc gggcggccga ttttcagcga aggatcgcta
    20821 atcatctcgt gccagtgcga aatccaacct acactgcgcg acagggcgaa gatgacggta
    20881 aacatttcgg tcgggatgcc cagcgcggac aggacgatgc cggaatagaa atcgacgttt
    20941 ggatacagtt tgcgttcgat aaagaacggg tctttcagcg caatctgttc caattccatc
    21001 gccagtttga atttcggact gtcttccaag cccaattcct tcaaaacttc atagcaggtt
    21061 tcgcgcataa tgctggcacg cggatccata ttgcggtaca cgcggtgacc gaagcccatc
    21121 agacggtatt tgcgttgttt cacaccttcc atgtatgcgg caacattaga cacatcgccg
    21181 atttcgtcca acattttcaa cacggcttcg ttcgcaccgc cgtgcgaagc accccacagg
    21241 caggcgatac cggcagcaat acaggcaaac gggttcgcac ccgaagaccc tgccagacgg
    21301 acggttgaag ttgaggcgtt ttgctcgtgg tcggcatgca aaataaagat gcggtcgagc
    21361 gcgcgtgcca aaacgggatt gggtttgtag tcttcacacg gcgtggcgaa catcatatga
    21421 aggaagtttt cggaataaga aagattattc ttcggataat tgaacggcag accgtttgaa
    21481 tagcggtagc acattgccgc aatggtcggg attttagaaa tcaggcggta aatcgcgatt
    21541 ttgcggtgtt cgggattgct aatgtccaag ctgtcttggt agaacgcaga cagtgcgccg
    21601 accacgccga ccatcatcgc catcggatgc gcgtcgcggc ggaacccccg gaagaaccaa
    21661 gtcagctgtt catgcaccat cgtgtggcgg cggacggtat tgtcaaattc tgccttttgc
    21721 tcgggagtcg gcagttcgcc gtaaatcaac aggtagcaga cttccaaata atcggacttt
    21781 tcggccagct gctcgatggg gtatccgcga taataaagca agccttgatc gccgtcgatg
    21841 taagtaattt tagactcaca gcttgcggtt gaaacaaatc cggggtcgaa ggaaaacaaa
    21901 cctgtatttt ttgtcagccc ccgaatgtca accacatcgt gcccgatgct ggcttccaat
    21961 accggcagct ccaaacctgc ctgacccggt acgttgagtt tgattgattt ggacatatta
    22021 ttgctccttt gcaagttata acccacgtta ctgcatccgc agcacgttct ttgccttcag
    22081 acggcttttg cattttgaaa tctgcctgca ggcagatttc agatgcctca tgcccgtctg
    22141 attttttcaa gcatcgggat aaggtgccct ttgtccgttt ccgaatgccc gttaatcaag
    22201 gcaagcaatt cttgatcttg aaattcaagg atttcggaaa actcggacag ctctttatcg
    22261 ctcaaatgct cgaattcttt ttccataaac ctgccgaaga ttaaatctaa ttccaacaat
    22321 ccccggcggg tttgaaaacg gattttccgt ttggcaatat cgtcaaaaac catcatttct
    22381 taaacggccc gtttcaacat aatctcttta atcttaccga tggctcgggt cggattcaag
    22441 tgtttaggac atacgtctat gcagttcata atggtgtggc aacggaacaa acggtatggg
    22501 tcgttcagat tatccaaacg ctcattagtg atggtatcac ggctgtccgc aatgaaacgg
    22561 taagcattca gcaaaccgga cggaccaacg aatttatcag ggttccacca aaatgacggg
    22621 caggcagtcg aacagcaggc gcacaaaata cactcgtaca aaccgtccaa ctctttacgc
    22681 tcttcctgag tttgcagacg ctctttgtcc gcatcaatcg gattatcgtt gacaacataa
    22741 ggtttgacgg aatggtattg tttgaagaac tgggtcatat ccacaatcag gtcgcggata
    22801 acaggcagac ctggcagagg acggatttta actggctgtt tcaagccacg cagatcggtc
    22861 aaacacgcca agccgttttt gccgttgatg ttcataccgt ccgatccgca aatgccttcg
    22921 cggcaggagc ggcggaaaga caaggtatcg tcttgtgctt tcaggcgtac caaagcatcc
    22981 aaaagtttca cgtcggtcgg ttccaattcc aactcgtaac gctgcatata aggcttggca
    23041 tcaacatccg ggttgtaacg gtaaatttca aaactcattt tttccatgag aagtgttcct
    23101 tttctcatca actgcccaat gattttgtat tataaattca agcagttcaa tgtgggtatg
    23161 gttagatttc agacgacctt tgaggcgaag accgtctgaa aacgcatcaa taaacgcgct
    23221 tggccggttt gatgtattcc acgctcaaag gcttggtgtg caccggtttg taggacaagg
    23281 tattggtatc tgaatggtac agcgtatgtt tcatccagtt ttcatcatct cgctcaggat
    23341 ggtcgtctga agcgtgcgca ccgcgtgatt ctttacgtgc ttcggcagac accaaagtcg
    23401 ctttcgccac ttcaatcagg ttatccaatt ccaaagcctc gatacgcgcg gtattccaca
    23461 ctttgctctt gtctttgatt tcggtacgtt tcacacgctc ggcaatcgcc atgacttctc
    23521 gaacgccttt gctcagaatc tcatcagtac ggaacacgcc ggcgtgcagt tgtacggagc
    23581 gttgcaattc gcggcgcaat gcatcaacgt tttcgccatc agtttgattg tccaaacgct
    23641 cgatacgttg gcgggtcagc tcgcccgcat ccgcaggcaa aggtttccag tcgctttgct
    23701 ctttgatgaa tttaatcatg ctgtcgccgg cagctttacc gaataccacc aagtccaaca
    23761 gggagttggt acccaagcgg ttcgcaccgt gtacggaagc acaagcgcac tcacctgccg
    23821 catacagacc ttttacaggc acttcgtatt cgtcgccttg aggaacgaca acttcgccat
    23881 ggtagttggt cggaataccg cccatcatat agtgggtagt cggcacaacg ggaatcgggt
    23941 ctttaatcgg atcgataccg gcgaactgaa tggaaatctc gcggatgccc ggcagttttt
    24001 ccataatttt ttctgcgccg atatggtcga ttttcagtaa gacatggtct ttgtttttac
    24061 cgcagccgcg accttcgtag atttccatcg ccatcgcgcg ggaaacaacg tcgcgagaag
    24121 ccaagtcttt tacggtcggc gcatagcgtt ccataaagcg ttcgccgtcg gcattcaaca
    24181 gaataccgcc ctcgccgcgt acgccttcgg taatcaacac gcccgcacct gccacgccgg
    24241 tcgggtggaa ttgccagaat tccatgtctt ccaacgggat acctgcacgc gcacaaatac
    24301 ccaaaccatc gccggtattc atataggcat tggtagaaga cgcataaata cggccgccgc
    24361 cgccggtagc aaacatcaca gctttagcgt ggaaaatata aacttcgccg gtttccattt
    24421 ccatggcggt tacgccgacg acatcgccgt tttcatcacg aatcaaatct tgtgccgtcc
    24481 attccacaaa gaattgcgta ttggcacgga cattttgttg gtacaaagta tgcagcatcg
    24541 catgacctgt acggtcggca accgcacagg cgcgttctac cgcgcgttta ccgtgttcgg
    24601 cagtatggcc gccgaaagga cgctgataaa ttttaccgct ttccacacgg tcaaaaggca
    24661 tacccatgtg ttccaactca attacggctt caggcgcggc gcggcacata aactcaatcg
    24721 catcttggtc gcccaaccag tcggaacctt tcacggtatc gtacatgtgc cagtcccaac
    24781 ggtcttcctg cacattaccc agagaggcgg aaataccgcc ctgcgccgct acggtatgcg
    24841 aacgggtcgg gaacacttta gacaaaacgg cacaattcag accggatttg gataattgga
    24901 gggctgcgcg taaacctgca ccaccaccgc cgacaatcac ggcatcaaac ttgcgaacag
    24961 gaaaacccat acttaccccc aaattacttt aattgaatac accaagcagc cgaccagcca
    25021 gacgatggtg gcaacctgca aaaacaaacg cacgccgaag ggtttgatat agtccatcca
    25081 caaatcgcgg atacccaccc aagcgtgcaa gaatacggcg atgaagctca cttgggtaaa
    25141 tacttttacc caagtttgac taaaaaatgc ctgccatgcc gaatattctt taggcagagc
    25201 aaatagaacc actaaaagtg caacggtata aatcaacata ataaccgcgg tcgcacgttg
    25261 catcgcccaa tcccgcaaac cgtaatgggc accggtcaat ttacgttcta ccataacaac
    25321 gctcccaaaa cgacagtcaa aaccaatgca gaagcaaata cggctttagc ggtattgcgc
    25381 gcagtattca gctcaaggcc tttgtgcgca tccaaaaata aaaagcggat accggcgaga
    25441 gaatggtgca gataagccca caatacaccg attaaaacca gcttgaccaa aggatgggaa
    25501 acaatggcac ggtaagtttc aaatgcagac tcttgactca gggtaccgga caggaaatac
    25561 agcaggaaag gcagcataat aaacagcccg accccgctga tgcggtgaag gatggaaact
    25621 atcccgggta tcggcagacg gatgttcggc aaatccagat aaacaggacg cggtttggca
    25681 gacatagtca attcctctgt agttatatat cgactcaaaa accgtaaacc cattgctaca
    25741 ctgggttaca taaacgttaa tttacctgtt ttatcccatt ttgaacagtg tattttaaaa
    25801 acaaactgat agattttgac aaacacgcgt ggaaacagca gattaaaaga aagattttta
    25861 gttttgccgc ccccttcccg acacggtatc agcatccgac cccatcccca aaaacaaaat
    25921 gccgtctgaa aaaatttcag acggcattgt tttgccatat cggtcaggct ttcaatttcg
    25981 catcggcatc gcgcaacgcg gtacgcaaac cttcctcgat aacgggatgg tagaacggca
    26041 tatccagcat ttgcggaacg gtcatcttca tttgatgtgc ccaagccaac aggtgcgcca
    26101 aatgttcggc ggccggacct acgatttctg cgccgatgaa gcggccggtg gctttttcgg
    26161 catacaggcg catatggcct ttgtttacca gcatcacgcg gctgcggcct tggtttttga
    26221 acgatacttc gccgatgaca aattcgtcgg cttggtattg cgcggcaacc tgcgcgtatt
    26281 tcaaaccgac aaagccgatt tgcggactgg tgaacactac gccgatggtg ctgcggcgca
    26341 aaccgccgcc gatattcggg tagcggcctg cgttatcgcc ggcaatcttg ccttggtcgg
    26401 cggcttcatg cagcaggggc agttggttgg acgcgtcgcc cgcaataaag atatgcggaa
    26461 tactggtctg catagtcagc ggatcggcaa cgggtacgcc gcgcgcatct ttttcgatat
    26521 tgatgttttc caaaccgata ttgtcaacgt tcggacggcg gcctacggct gccagcatat
    26581 attcggcaac aaatacgcct ttttcgccat cctgctccca atggacttct acattgccgt
    26641 ctgcgtcgag tttggcctcg gttttagcat ccaaatgcag tttcaattct tcaccgaaca
    26701 cggctttcgc ctcgtctgaa acgacggggt cggaaatgcc gccgatgatg ccacccaaac
    26761 cgaaaatttc aactttcacg cccaaacggt gcaatgcctg acccagttcc aaaccgataa
    26821 cacccggccc gaacacggca acgcgcttag gcagcgtatc ccatgagaaa acgtcatcgt
    26881 tgataatcaa acgattgccc aaagactgcc attgcggcag gatgacggga cgcgaaccgg
    26941 tagcaatcac gaaacttttt gccgtaattt gagtatgctc gtcgatttgg acggtatgct
    27001 cgtcgataaa tttagccgaa cccataatgc gcttgtcggc aggccactct tccacatcgg
    27061 caacgacaaa gccgacaaaa cggtcacgct cggatttaac gcgctgcatg acctcttcac
    27121 cgttgacgac gatgctgtct ttgtccaaat gcacgccgaa cgggtcggta tgcaatgcgt
    27181 ggtggcgcgc ctctgcggcg gcaatcaaga gtttggaagg catacaaccc acgcgcgcgc
    27241 aggttgtgcc gaacacattg ttttcaatca ggtaaacatt atccgaatgt aaacgggcat
    27301 tgcgaaacgc acccataccg gcagtaccgc cgccgagtac gacgacatcc gcttgaattt
    27361 ttttcataat ctttgtcctt ccgttttcgt ctgaaacatt gccgacacag aacatatcgg
    27421 tttcagacgg catttaaata ttttcccaat caaaaacatc attcagataa ataaaaacaa
    27481 aatttcgatt gagtgcaaca atatccaaat tctcttataa atatagagtt attcggagcg
    27541 gcagtgtaga accgcccgaa taactctatc cggtatatca gacagtttaa tcaaaccgcc
    27601 ttaagcaaac agctatcagt ttttagccaa gtaagcttcc aaatcttcgc tgccgccgat
    27661 gtatttaccg ccgatgaaga cttgaggagc agtcatcttg ccggtaatgg cgcgtacaga
    27721 agtaacggtt gcatctttgc ccaatacgat ttcttcgtat gacaaaccct tgtcttgcaa
    27781 agcttgtttg gctttggcgc agaattgaca acctggttta gtgaaaattg ccacagactc
    27841 ttgagccttc caatcaggag caacgaattt cagcatagta tctgcatcgg atactttgaa
    27901 cggatcgccc ggttcttcag gctcgatgaa cattttttca accacgccgt cgttaaccag
    27961 catggagtaa cgccaagagc gtttgccgaa gcccaagtct tctttaccga ccaacatacc
    28021 catgccttcg gtaaattcgc cgttgccatc aggaatcatg tagatgttgt cagattcttc
    28081 ttcggcagcc caagcgttca ttacgaaagt atcgtttaca gatacgcagt agattgcatc
    28141 aacgccgttt tctttgaacg cgccgaacaa ttcattgtaa cgtggcaaat gggaagaaga
    28201 gcaagtcgga gtaaatgcgc caggcagaga gaatacgact acttttttgc ctttgaacaa
    28261 atcatcggta gacacatctt tccaagtgtc gccgacgcgg gtgcggaata ctacggaagg
    28321 tactttttga ccggtacgat cttgcaaagc cattttgagc tccttaatag ataggtttaa
    28381 gaaatgattg tgaatcgctg ggcattctac cccgaataag tcgatttgta taatttatag
    28441 ttcaaataat attaattatt caaaactatt aattaccaac ctcgaccaac ttcttgcagt
    28501 atataaagcc atgcctaaaa aaaacaagtc ttatttgaga aaaattctta gaaactcccc
    28561 tacgctcatt tgaaaacaga aatgccgtct gaaaagtatt cagacggcat ctgatcacgt
    28621 tatccctgct tatttagcca gttcggcacg cagtttgtgg gttacgttca tcatcacttg
    28681 gagttgttcc agagtttctt tccagccacg tgttttcagg ccgcagtctg ggttaaccca
    28741 cagacgttca accggtacaa cctcgatggc tttgcgcaac agatgttcca cttcagcttc
    28801 agtcggtacg cgcgggctgt ggatgtcgta aacgcccggg ccgatgtcgt tcgggtattt
    28861 gaattcgccg aacgcggtca agagttccat gtcggaacgt gaagtttcga tggtgatgac
    28921 gtccgcatcc attgcagcaa tcgcaggcag gatgtcgttg aactcagagt agcacatatg
    28981 ggtgtggatt tgagtgctgt cttcgcaacc agtagaggac aggcggaagg attcgccagc
    29041 ccagttcagg taggcatccc aatcggcacg tttcaaaggc aggccttcgc ggatggcagg
    29101 ttcgtcgatt tggatgactt tgatgccggc tttttccaga tccaatactt cgtcgttcag
    29161 ggccagtgcg atttgtttgc atacagtaga gcgaggaata tcgttgcgga caaaagacca
    29221 ttgcagaatg gttacagggc cggtcaacat gcctttcatc gggcgtttgg tcaggctttg
    29281 tgcgtaagta gaccaagcta cggtcatggc ttcaggacgg cttacgtcac caaagatgat
    29341 cggtggttta acgcagcgtg agccgtagct ttgtacccag ccgtattggg tgaatgcaaa
    29401 accgctcaac aattcgccga agtattcaac catgtcgtta cgctcggctt cgccgtgtac
    29461 cagtacgtcc aagtccagtt tttcttgctc ttcaaccacc aaggcgattt cttttttcat
    29521 cgcggcttcg taatcggcgg cagacagttc gccttttttg aaggcggcgc gtgcctggcg
    29581 gatttcggta gtttgcggga aagaaccgat gttggtagtc ggcagcagag gcaggttcaa
    29641 ccatgcttgt tgcgctttga tacgatcggc aaatggagat ttgcgttggt ctgcgttggc
    29701 aggcaaatca gccaggcgtt tggcaacgtc tgcacggtgg atttcgctgc tgttggcacg
    29761 agaatcggca gcagcttggc tagcggccag ttcttcggca acggaatcac ggccttcatt
    29821 taatgcggct ttcagaacgc gcaattcttg ggttttttgc agggtaaatg ccaaccaaga
    29881 gtacaagtcg ggtttgttgg ctttcagttt ttcttcaact gacaagtcaa atggagtgtg
    29941 cagcagagag caagagctgg aaatccacaa acggtcgccc agtttggctt gcagaggctc
    30001 aacagtttcc aaaactttgt tcaggttggc gcgccaaatg ttgcgaccat caatcacgcc
    30061 ggcagacagg actttgtcat agtcggcgaa cgcgtccagt tgctcgggag cgcgtaccaa
    30121 gtcgatgtgc aggccgtcaa caggcaggga tttcaacaat gcggcgtgtt cggcaacaga
    30181 accaaagtaa gtgctcaaca ggattttggc acttactttg ttcaaagtgg cgtaaacgtc
    30241 tttgtatgct tctacccatt ctttaggcag gtcaacagtc aaagcaggct cgtcgatttg
    30301 aatccactcg gcaccggctt caaccaaagc agtcaggatt tcaacgtaaa caggcagcag
    30361 tttaggcaac aggctcaggc ggtcgaattc gactgcgcct ttttctttac ccacccatag
    30421 gaaggtcagc ggaccgacga tggtcggttt ggctttcagg cccaaagctt gggcttcttg
    30481 caactgttga acgtaatgtt tggcgttggc tttgaattcg gtatcggcgt ggaattcagg
    30541 caccaagtag tggtagttgg tgtcgaacca tttggtcatt tcgatagcaa attggtcttt
    30601 gttaccacgc gccagttgga agaattgttc caaagacagg ttttggctgt cgaagccgaa
    30661 gcgggcaggg atggcaccgg tagcaacttg caggtcgagg atgtgatcgt agaaagtgaa
    30721 atcgcctaca gcaacgtaat cagcgttggc agcagcttcg tgtttccagt ttttctcgcg
    30781 caagtcttta gcaacagcca gcaattcttg ctcgctgatt tctttgcgcc agtatttttc
    30841 ttgtgcgaat ttcaattcgc ggaaggcgcc gacacgcggg aagcctgaga aatgtaatgt
    30901 tgtcatgttg aactctccag ttggattttg taaggaccgg ttcaatccga atcggccggt
    30961 attgtaaatc agaatggctc ggatggggcg gcgttttgct tttcagacgg ccctggatgc
    31021 cgtctgaagc cgttttcggc tttaagggcg cacacctaaa atatggcaga tggcgtaagt
    31081 cagctcgctg cggttaagcg tatagaagtg gaaatctttc acgccttcgc gggacaggac
    31141 tttgaccata tcgatggcga tacttgccgc caccagattg cgcgtacctt ggtcgtcgtc
    31201 caaaccttca tacatttgcg acagccagct tgggattttg acgttggtta cttgagccat
    31261 tttaccgagc tgcttgaagt tggtaacagg caaaataccg gggacgattt ccacatcgat
    31321 acccaacatc acgcagcggt cgcggaagcg caggtagcgt tccacatcga agaagaattg
    31381 ggtgatgacg tggttcgcgc ccgcatcgat tttgcgtttc aaattaatca ggtcggcttg
    31441 tgcggatttc gcttcgggat gcacttcggg atatgctgct acagagatgt cgaagtcggc
    31501 gacggagcgt aatagcttaa ccaagtcttc ggcgtaaaac ggttttttct cataaccggc
    31561 cggctcgtct ccgcgcaggg cgacaatgcg gcggatgccg ctgtcccaat aatctttggc
    31621 aatttggcgc aattcgtcgg gagaagcgtc gataccggtc aggtgcggcg cggcttccaa
    31681 gccggtttcc tgtttgatgc gtttgacgat gccgtgcgtg cggtcgcgtt cgccggaatt
    31741 tgcgccgtaa gttacggata cgaacttggg atgcagggtt tgcagacggt ggatggagtc
    31801 ccacagcatc gtttccattt gttcgttttt cggcgggaaa aattcaaacg aaacgttaat
    31861 gtcgcctttc aaatcagaaa gactgttatt taacgcattg atttcttttg cgtaattcat
    31921 ggctgcgtac cttttattat cttttatatg atgtttcagg caacataata aagttgcgcc
    31981 aaacatgagt caatttcaaa attttcagat ttatcatgaa aatttctaat atattacaaa
    32041 aatagttaaa tttccttgta caagccttct aatttatgat aaagtttgtt atgttataac
    32101 aattcaacta aggcgatata tgaaaccgaa tattcatcct gacaattatc gaaccgtcct
    32161 ttttttcgac agcagcgcca atgaaggctg gttgattcgt tcttgcgccg aaacacacgg
    32221 aaaaacgatg gtttggacag acggcaaaga atatccgctg ttttcgctgg atacttcttc
    32281 cgcatcccat cccgtctata ctggcaaaca gcgcaacgtc aacaccgaag gccgtgccag
    32341 caaattcaac caacgtttcc aatccgtcat gtcatcgttt agaaaggaca aataatgcag
    32401 gttctgtctt cactcaaaac cgccaaacaa cgccaccgcg actgccaaat cgtcagacgc
    32461 aggggcaaag tttacgtcat ctgcaaaagc aatccgcgtt ttaaagcacg ccaacgctga
    32521 ctccctgccc ttcctgcttc gg
//
LOCUS       DAXHTY010000011        32374 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXHTY010000011 DAXHTY010000000
VERSION     DAXHTY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.011"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(68..814)
                     /locus_tag="ACFNMK_000445"
     CDS             complement(68..814)
                     /locus_tag="ACFNMK_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6363775.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(798..977)
                     /locus_tag="ACFNMK_000446"
     CDS             complement(798..977)
                     /locus_tag="ACFNMK_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363776.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(993..1664)
                     /locus_tag="ACFNMK_000447"
     CDS             complement(993..1664)
                     /locus_tag="ACFNMK_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC6363777.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFNMK_000448"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFNMK_000448"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC6363778.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFNMK_000449"
     CDS             complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFNMK_000449"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC6363779.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFNMK_000450"
     CDS             complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFNMK_000450"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC6363780.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTLGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKRADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIHPLLVQGDARNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFNMK_000451"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFNMK_000451"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC6363781.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAEHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="ACFNMK_000452"
     CDS             complement(4571..5881)
                     /locus_tag="ACFNMK_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6363782.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLSETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFNMK_000453"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFNMK_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC6363783.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="ACFNMK_000454"
     CDS             complement(6056..6355)
                     /locus_tag="ACFNMK_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363784.1"
                     /translation="MHYFSIHDQWGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="ACFNMK_000455"
     CDS             6706..7902
                     /locus_tag="ACFNMK_000455"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6363785.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTAGSKAIAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8538..10379)
                     /locus_tag="ACFNMK_000456"
     CDS             complement(8538..10379)
                     /locus_tag="ACFNMK_000456"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC6363786.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10470..10940
                     /locus_tag="ACFNMK_000457"
     CDS             10470..10940
                     /locus_tag="ACFNMK_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC6363787.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKHGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAENQARLNPHEIA"
     gene            complement(11013..11825)
                     /gene="hpnC"
                     /locus_tag="ACFNMK_000458"
     CDS             complement(11013..11825)
                     /gene="hpnC"
                     /locus_tag="ACFNMK_000458"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC6363788.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(11912..12394)
                     /locus_tag="ACFNMK_000459"
     CDS             complement(11912..12394)
                     /locus_tag="ACFNMK_000459"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6363789.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDVGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            12867..13193
                     /locus_tag="ACFNMK_000460"
     CDS             12867..13193
                     /locus_tag="ACFNMK_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363790.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPATEAPAA
                     EAPAAEAPAAEAPAAEAPAAEAPATEAPAAEAPAAEAAATEAPAAEAAATEAPAAEAP
                     AAEAAK"
     gene            13303..14670
                     /locus_tag="ACFNMK_000461"
     CDS             13303..14670
                     /locus_tag="ACFNMK_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC6363791.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLTQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(14851..16878)
                     /locus_tag="ACFNMK_000462"
     CDS             complement(14851..16878)
                     /locus_tag="ACFNMK_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC6363792.1"
                     /translation="MKLEASRSLKKSFIVSLFFSILYTSPLLAVDYVYDNSKLTNEQI
                     DRLKKLRDKSSEYWKQETYLLKPTNGSSQNSLKVPALYSKTHQFENINNSKKISFYDK
                     EYTEDYLVGFARGFGVEKRNGEEEKTLQQYFKDYVNTENSNNDKCKQPSLGSYAQMLI
                     KSDIFSLAPQIKNSHINSEILSVGNYIEWLRPTLNQLTSWQEQLYSGLDPFHYIDVTD
                     NSHVIGQTISLDEFRLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAGGA
                     RFRFAYGLKDKVPETPILTFEKNITGTSDIIFEGKALDNLKHLDGHQIIKVNGTADKH
                     AFRLSGKHQKGIYTLSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFALRLNDKNSD
                     IFDRTLPRKGLWLRLISGHLSQDVQGKTAPVEGNRKGVQLGGEVFTWQNESNQLSIGL
                     MGGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWMQYQRFRH
                     RINTEDATERFTSKGITASIEAGYNALLAEHVTKKGNSLRVYLQPQAQLTYLGVNGKF
                     SDSENAHVNLLGSRQLQSRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEMDGE
                     RRVINNKTAIESQLGVAVKIKSHLTLQASFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(17251..17697)
                     /gene="smpB"
                     /locus_tag="ACFNMK_000463"
     CDS             complement(17251..17697)
                     /gene="smpB"
                     /locus_tag="ACFNMK_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC6363793.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(17752..18762)
                     /gene="waaF"
                     /locus_tag="ACFNMK_000464"
     CDS             complement(17752..18762)
                     /gene="waaF"
                     /locus_tag="ACFNMK_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC6363794.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAVIALAT
                     GIAKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(18893..18968)
                     /locus_tag="ACFNMK_000465"
     tRNA            complement(18893..18968)
                     /locus_tag="ACFNMK_000465"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18933..18935),aa:Asn,seq:gtt)
     gene            complement(19159..19911)
                     /locus_tag="ACFNMK_000466"
     CDS             complement(19159..19911)
                     /locus_tag="ACFNMK_000466"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC6363795.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLEASYPDSLNELVCAAIATPLGEMLAVFGSKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPNAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            20222..20746
                     /locus_tag="ACFNMK_000467"
     CDS             20222..20746
                     /locus_tag="ACFNMK_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFC6363796.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGDNAPPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHWVVADIPADVRRRN
                     AASLQLSRCANIADDQPAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     TLCGTFSSASTAAA"
     gene            complement(20971..21114)
                     /locus_tag="ACFNMK_000468"
     CDS             complement(20971..21114)
                     /locus_tag="ACFNMK_000468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363797.1"
                     /translation="MPSNYFHTRERLGALGGIVQHHTALYRSVAVYEPAVLIVRRGSK
                     KLR"
     gene            complement(21440..21601)
                     /locus_tag="ACFNMK_000469"
     CDS             complement(21440..21601)
                     /locus_tag="ACFNMK_000469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363798.1"
                     /translation="MPSEKKMGCRIWMSDHLIRPNVGLRYSGLNSNQYGVASPCRTIC
                     TVCGFVALS"
     gene            complement(21591..22736)
                     /gene="dapE"
                     /locus_tag="ACFNMK_000470"
     CDS             complement(21591..22736)
                     /gene="dapE"
                     /locus_tag="ACFNMK_000470"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC6363799.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEAL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNKYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYEGILARLLAGNAV"
     gene            complement(22849..23469)
                     /locus_tag="ACFNMK_000471"
     CDS             complement(22849..23469)
                     /locus_tag="ACFNMK_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HFC6363800.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     RLQNDEDFFCIGKHIERDDLGRQDVVKWIAETVEDLLSLYEACHGK"
     gene            complement(23503..24012)
                     /locus_tag="ACFNMK_000472"
     CDS             complement(23503..24012)
                     /locus_tag="ACFNMK_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6363801.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQLVLQTIRQITRYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHVSLHGN
                     WDAVAAEFAKLEADNAYIPDAEAFKRFVAGYDVHLAIEEPLFDMGKMFIPKEKLTEIG
                     EIMAARRRR"
     gene            complement(24107..24658)
                     /gene="azu"
                     /locus_tag="ACFNMK_000473"
     CDS             complement(24107..24658)
                     /gene="azu"
                     /locus_tag="ACFNMK_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC6363802.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKTSMGH
                     NIVIGKTEDMDGIFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(24939..25361)
                     /locus_tag="ACFNMK_000474"
     CDS             complement(24939..25361)
                     /locus_tag="ACFNMK_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC6363803.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAAKLEKRHPELAEALANLVRRHGA"
     gene            25492..28242
                     /gene="secA"
                     /locus_tag="ACFNMK_000475"
     CDS             25492..28242
                     /gene="secA"
                     /locus_tag="ACFNMK_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC6363804.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            28388..30160
                     /gene="dnaG"
                     /locus_tag="ACFNMK_000476"
     CDS             28388..30160
                     /gene="dnaG"
                     /locus_tag="ACFNMK_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC6363805.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            30347..32275
                     /gene="rpoD"
                     /locus_tag="ACFNMK_000477"
     CDS             30347..32275
                     /gene="rpoD"
                     /locus_tag="ACFNMK_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC6363806.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         8193 a         9195 c         7608 g         7378 t
ORIGIN      
        1 ataaatttac cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa accttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaagg aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccatataccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgga cgctgacttt cctgccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacaaggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatctctga accggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tatgccgttt tcgagcacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggctg atcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaaggg
     1981 aacgcgcgct ttcgacgact tccacgggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag atgaattttt ctgatgcgct ggcgacgatt ttttcgttga
     2101 ggtgcgcgcc gccgccgccc ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 cgatatagac cgccaaaccc gatacttcgt tcagggaaac gacgggaatg tcgtactggg
     2221 caagcagttc gctggatttt ttggaggttg aaacagcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgagccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatccgt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggtggat gcccaatttt tcgacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagcccgg cgcggaaaag
     3181 ctgcggcgtt tgtgcctgcc aaaggctcgt ccgctcgacg gttgcactaa tgttcccacc
     3241 gtccgcgcgc ttgagcgtat cggcaacggg aattgccaaa atcccacctt cggcagcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcaac gcgcggcatc
     3361 gtgtaccaga atattgtcgg tttccgccgc caaaccggtt tccaacaatt ttgccacacc
     3421 gtttcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacgccctca tgccgttcaa aaatcccaag cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgttc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcaccacg
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattatgg
     4141 ataatcagtt ccgcaccgcg cagaaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctctatc gtcagaccgt gcaccctcgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcta aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc ggcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaagt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaggcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcagcc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacaggg caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc gtttcactca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacagacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacaggtgct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgcgcctc tgactcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtccccatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat gcccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgctagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc catcaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaataaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aggcacgctt tggcaacgcc
     7801 ggcgtgatta ccactgccgg cagcaaagcc attgccgtgg tcattccgac caacgaggaa
     7861 ttgatgattg cacacgatac cgcccgcttg agcggtctgt aagattcaat ccccacgcga
     7921 actgcctccg aaaacggagg cagttttttt atccggcttt ccatgcttaa acagcaccgc
     7981 ctcttttcag acgttgacgg ttgcagccgc ttacctgaac cttatagtgg attaacaaaa
     8041 accagtacag cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctcaagca
     8101 ccaagtaaat cggttccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
     8161 tttgttaatc cactatatcc gactttccat gcttaaacag cactgcctct tttcagacat
     8221 tgacggttgc agccgactac ctgaacctta atgattgact attttttaat catgttatta
     8281 ttttccataa aatacatgac attaagatgt ttttccacaa aagatacaca caccggcaaa
     8341 caccggctgt gtttatcttt tcttatgcct attttttaat catcgtattt ttatctttta
     8401 atttcaatac gcaaactaac ttatacacac ggttttcaca tctttggact gcttccgtgt
     8461 gtatagcgga tattgccgtt ttcctttctg acaaaaatgc cgtctgagaa cttcagacgg
     8521 catttgaaac atcggaatca gcggttttgt tcataccact cgataaactt gtctgctttg
     8581 acaaaaccca gcagcggctc gctgcggctg ccgtcggagc ggacgacaaa cacgcccggc
     8641 ggcccgaaca gaccgtattc tttcaacaac gcctgatgtt cgggcgtgtt ggcggttacg
     8701 tcgatttgga aaaagcgttc catatcgact gcctgatgca cttccggctg attaagcgtg
     8761 taagccgcca tttctttgca ggaaatgcac cagtcggcat aaaaatccaa aacgacgggt
     8821 ttgtcgggat gttctttcaa cgccgtatcc atcgctgcct tcagcgcggc agtatcggca
     8881 aacatttttc cgtgttccga agatttgcct gcttcggccg gcggattgag ggtcaggaaa
     8941 tggtgcagcg cggtcgtttt gccgtttgcg ccctgccagc cgaaccacgc gccgcctatc
     9001 agcaatatgc cgcccaatgc gaatgccaca gctttcggac ggcgtttctg cctgcgtccg
     9061 ttgaccagca gcatacaggc aggaaccagc atcagcagcg tgtacagcgc gacgacgaga
     9121 taatagggca agtgcggcgt ggcgaggtaa acggcgacgg caagcaggat aaagccgaaa
     9181 gcgtatttga cggcattcat ccaatcgcct gccttaggca ggatatgccc gccgaacgtg
     9241 ccgatggcaa tcagcggaac gccggtgccc aacgccaaag tgtaaagtgc caaaccgcct
     9301 aaaaccgcat cgcccgtctg accgatgtag cccaaagcaa atgccagcgg cggggcgacg
     9361 cacggcccga caatcagcgc ggacaatatg cccataataa agacggaaac gattttaccg
     9421 cctgaaagcc tgctgctttg attctgaaaa tacgactgca cggcgttggg aagctggatg
     9481 ttgaacagcc cgaacataga cagtgccaag acgaccatca aagccgatgc cgccaacacg
     9541 acccaagcct gctgcaacca tacggtcagc agcgcgcccg tcagtccggc aacaatgccg
     9601 accagcgtat aagtcagagc caaaccctga acataaacga cggacagcac aaacgcccgc
     9661 gccttgcccg cctttttgtc gccgaccaca atactggaaa caatcggcaa caggggatac
     9721 atacaggcgg taaaactcag acccaaaccg gcgagaaaaa acgccaaaag attggcgttg
     9781 agcgtatccc aagacagctt gaaacggctg tcgccgccct catccccctt cgggggcggc
     9841 agcgccccgc tgccgttttg agaggaaggc tgcaaaaagc gatctttggc ggatgccggt
     9901 tcgtcggttt gcggatggta agtgccgttg ccggaaatat caaactcggt atccacgggc
     9961 ggatagcaca cgccggcttc ggcacagccc tgataggtca aaaccaattt atacggttcg
    10021 ccgacagcct ttgcataagg aaaggcaacc tgcgcttcgt gatggtaaac cgtctgcctg
    10081 ccgaaaaact cgtcttcctt ctcttcgccc ttgctgaaag aaggctgtcc caacaaatcc
    10141 gccggatcgg tcttgccgac gattttcgcc tgatacatat agtatccgtc ggcaatcctg
    10201 aaacggacgt tcacaccgtc gtcggcaacg gcaagctccg gcacgaatgc cttttccggc
    10261 ggcagcagat cgttcgcatc cagcgcgaaa gctcgtccgc acaacatcaa aaatacggcg
    10321 aacaggcaaa tcagtttttt cataatcgaa tccgtttcag acaaataatt tgtctgcatt
    10381 ataaatggta aggttgacgg tgggatttaa tttatgtaaa acccgccatt atccgaacct
    10441 atttccataa acatcttatc gaacccgcca tgtacgatgt caatacccac gatgtccgcc
    10501 gctttttcgc ccgcgtgtgg cagcagcggc tcaatccgct gcaactgagc gcactggaac
    10561 agaaagccct ccgcattatc gaagcccatc ccgaatacca ccgttatctc gaacgcatcg
    10621 aagaccatct ggacaccgac tggctacccg aaaacggcga aagcaacccc ttcctgcata
    10681 tgtcgctgca tctgtccgtc caagaacagg cgggcataga ccagccgcac ggcatacgcg
    10741 caatccacga caccctgtgc gccaaacacg gctggctgga agccgaacac gaaatgatgg
    10801 aggcactggc ggaaacactg tggacggcgc aacgctacgg caccggtttg gatgtcaatt
    10861 tctacatgac ccgactgcgc aaactcatcg gcttgggtgc agaaaatcaa gccaggttga
    10921 acccgcatga aatcgcctga ccataccaac cgcctgcaaa atgccgtctg aagcggaaca
    10981 acccctttca gacggcattc attttccccc aatcatttcc acaacgcctt tttcagcata
    11041 atcaaccaat ccttcttatc caaaacgggg cgttgtgcaa acacatcgta tcggcacgcg
    11101 tccagtttct gcaaaatcaa ctgcgccccc aacacaatca tacggagttc caaaccgata
    11161 cgccccttca gttcccttgc caaaggcgaa cccgccttca gcatacggaa tgcgcgtcgg
    11221 cactcatacg ccatcagccg ctgaaacgcc gcatccgccc gtcctgccgc aatctgttcc
    11281 tcagaaacac cgaatttcaa caaatcgtcc tgcggaatat aaaccctgcc cttttgccaa
    11341 tccacagcca catcctgcca aaaattcacc agttgcaaag ccgtacagat gccgtcgctt
    11401 tgcgccacgc acaccgcatc cgttttcccg tacaaagcca gcataatgcg tccgacaggg
    11461 ttggcggaac gccggcagta atcggccagt tcgccgaaat gcgcgtaccg cgttttaacc
    11521 acgtcctgcg aaaacgccga aagcaaatca taaaacggct gcaaatccaa accgaacggc
    11581 acaaccgcct cggcatccaa tcgtgcaatc aaaggatgcg ccgaccggcc gcccgatgcc
    11641 aacacgtcca actcgcgctg caaaccctcc aaccccgcca acctggcttc agacggcata
    11701 ctgccctcgt ccgccatatc gtccgccgtc cgtgcaaacg cgtacaccgc gtgaaccggc
    11761 ttcctcaacc tgcgcggcaa aatcagcgaa ccgacgggaa aattctcata atgcccaacc
    11821 gacatacctt ctccatccat caaacaaaat gccgtctgaa acggaacaaa cccttttcag
    11881 acggcatcag atacctctaa gctgccggca atcagtggtg gtgatgaccg tgcgggccgt
    11941 ggacatgacc gtgtgcgatt tcctcatcgg atgcatcgcg cacgctttca accgtagcct
    12001 taaagcggat tttcatgcct gccaaaggat ggttgccgtc caccaccgcc ttgccgtcgg
    12061 caacatcggt tacacgatag acgacaacat cgccggtttc aggatcgtcg gcttcaaaca
    12121 tcatgccgac ttcgacttca acagggaaca cgcccacatc ttcaatacga accaactccg
    12181 gatcctgctc gccgaacgca tcgtcgggcg acagcgccac atcgaccgta tcgccggcat
    12241 ccttaccgtg caacgcctct tccaccaaag ggaaaatgcc gtcgtaaccg ccgtgcagat
    12301 acgcaatcgg ttcttcggtt ttgtccaaaa gctgattgtt ggcatcatac atctcataat
    12361 gcagcgaaac cacggaattt ttcacgatag ccatatttgt cctttcagga acagcagatt
    12421 aattacaggc gcattctaac acaaccgccg cgccggccga ttaccgttaa cctattcata
    12481 aactgtacag cacatatttc aatgtaaatc tttgttattt tattgcggtg taactttttt
    12541 acaacattct taaaaccatt ccgacctgtc tgccgacttt cccaatccgc cttaataaat
    12601 catacaagat actgaaatta tattaatctc tataatattt atccctatcg aatttttaac
    12661 agcaaaaccg ttttacagga tttatcaatc cgcccgccag aaaacttttc attcaaacct
    12721 ttttcccatc tgtacgacat tgcaatccct tattccatag tgcataatta cgcaaattca
    12781 gcgatgaatt tccaacccgg tttgtagtat ggtcgataaa gacctatttg tttcaataat
    12841 ttaaattggt tctaaaggtt actaaaatga aaaaatccct gtttgccgct gctttgttgt
    12901 ctttggttct ggcagcctgc ggcggtgaaa aagccgctga agctcccgct gctgaagcac
    12961 ctgccgccga agctcccgct actgaagcac ctgccgccga agctcccgct gctgaagcac
    13021 ctgccgccga agctcccgct gctgaagcac ctgccgccga agctcctgct actgaagcac
    13081 ctgccgccga agctcccgct gctgaagctg ccgctaccga agcacctgcc gctgaagctg
    13141 ccgctaccga agcacctgcc gctgaagctc ctgctgccga agctgcaaaa taagcatttt
    13201 ccgcttgcaa aaaagcagga tacgttcagt atcctgcttt tttgattttt cagacggcat
    13261 cagattccct tcctcaatct tctccctacc cttccgacaa acatgcctga ccttcatacc
    13321 gaattttccc gactcctgcc ggcagatgaa attgccgaac tttctccgac gcttttaaaa
    13381 gaccagcgca accgctttac gtctgcacca gacatcattt tgcagccgcg cagcgttgaa
    13441 agcgtgcaaa ccattatgcg tttttgttac gaacaccgta ttccggttac gccgcaaggc
    13501 ggcaataccg gtttgtgcgg cgcggcagta tcggaaaacg gcgtattgct gaacctttcc
    13561 aaactcaacc gcatccgcag catcaatttg tcagacaact gcataaccgt cgaagcaggt
    13621 tccgtactcc aaaccgtcca acaggcagcc gaagcctcaa acaggctgtt cccactcagt
    13681 ctcgccagcg aaggctcctg tcaaatcggc ggcaacatcg cctgcaatgc cggaggtttg
    13741 aacgtattgc gttacggcac gatgcgcgac ctggttatcg gtttggaagt cgtcctcccc
    13801 aacggcgagc tggtttccca tctccatccc ctgcataaaa acaccaccgg ctacgacctg
    13861 cgccatctgt ttatcggcag cgaaggcaca ctgggcatca tcactgccgc cacgctcaag
    13921 ctttttgccc gtccctcgga caaggcaacg gcatgggtcg gcatacccga catcgaatcc
    13981 gccgtccgcc tgctgaccga aacccaagct cactttgccg aacgcctatg cagttttgag
    14041 ctgatcggcc gttttgccgc cgagttgtct tccgaattca gcaaattccc cctgccgaca
    14101 cattcagaat ggcatatttt acttgagttg accgactcat tacccgacag caatcttgat
    14161 gatcggcttg tcgaatttct ttataaaaaa ggatttaccg acagcgtatt gacacaaagc
    14221 gaacaagaac gtatccatat gtgggcattg cgcgaaaaca tctccgcatc gcaacgcaaa
    14281 ctgggcacca gcatcaaaca cgatattgcc attcctatcg gacgcgttgc cgactttgtc
    14341 cgccggtgcg ccaaagattt ggaacagaat tttaaaggca tacaaatcgt ctgcttcgga
    14401 catcttggcg acggcagcct gcactacaat actttcctgc ccgaaatcct cagcaatgaa
    14461 gtctatcgtt acgaaaacga catcaacgac gcagtttatc gcaacgttct tgcctgcaac
    14521 ggcacgattg ccgccgaaca cggcataggt atcatcaaaa aacagtggct ggacaaagta
    14581 cgcacgcctg ccgaaatcgc cctgatgaaa agcatcaaac aacaccttga tccatataac
    14641 attatgaatc cgggcaaact gcttccgtaa ccggcatttc tgatttgcat acacaacaaa
    14701 gaaagggaca atagatccga ttgtcggttt agcgcgagct cgtgagtgcg gtaaaaaatt
    14761 ggtggaaatg acacgaaaaa tgaccgcatt tttaaaataa aaaaatcggc agtgaatttc
    14821 cctgccgatt ttattttgtt acaacttaac ttaaaacgtc cactgcaaat tcaacgcacc
    14881 ttgtttagcc tgatgatgtt tgcctgtttg gcggttgaat gaggcttgta aggttaagtg
    14941 agatttgatt ttcactgcta cacctaattg gctctcaatt gccgtcttat tgtttatcac
    15001 tcgacgctct ccgtccattt ccacaccgaa aggtttgttg tggtaaagcg cgttcacagc
    15061 ggcgaaaggt tcaatagcga tatttttata gagtgaaaat tgcgctttag cttgaacgcc
    15121 cactcggctt tgtaattggc gggagccaag taaattcacg tgggcatttt cgctatcgct
    15181 gaattttccg tttaccccca aataagtcaa ttgtgcctgt ggttgtaggt aaacacgaag
    15241 gctgttgccc tttttagtta cgtgttccgc caataaagcg ttgtaacctg cttcaattga
    15301 ggcagtaata ccttttgaag taaaacgttc tgtagcatct tcagtgttga tacggtggcg
    15361 gaagcgttga tattgcatcc agctatccgc atacgcacct gtctgtttgt cctgaagctg
    15421 atgccaagtg gcgtaaacgc ctgcaccaaa gcctttcaca tttcccgttg taagattgtc
    15481 tgtatctgga ttgcggaaag tactgcgttg ttctgcctgt ccgcccatta agccaataga
    15541 aagttgatta ctttcgtttt gccatgtgaa tacttcgccg ccgagttgca caccttttcg
    15601 atttccctct actggtgcgg ttttaccttg tacatcttgg cttaagtgac cactaattaa
    15661 acgcaaccac aagcctttgc gtggtaaagt gcggtcaaaa atatcgctgt ttttgtcgtt
    15721 caaacgcaag gcgaataagg tattggcggc ttgagcctgt tgtgcataaa tcgcaatatc
    15781 atcgcgttct tgcactttgg taaaaaagcc ctctgggcgt tgttgtaaag aaagcgtata
    15841 aattcccttt tggtgtttgc cagaaagacg gaatgcgtgt ttatctgctg tgccatttac
    15901 tttgataatt tgatgcccgt ctaggtgttt taaattatcc aacgcttttc cctcaaaaat
    15961 aatatctgat gtgccagtta tatttttttc aaaagttaaa attggtgttt cagggacttt
    16021 atcttttaga ccgtaagcaa aacgaaaacg agctccgcca gcataatctt cttttacgag
    16081 taaactttca cttttagtgt taaaacggat gtctgcattc gttgttttta attccccaac
    16141 atcagaatcc caacggggtt cccagagaga attttctaag cggaattcat ccaaactaat
    16201 tgtttgtccg ataacgtgtg agttgtctgt aacatcaata tagtggaatg gatctaaacc
    16261 agaatatagc tgctcttgcc aactagtaag ctgattaagt gttgggcgaa gccattcgat
    16321 ataattacct acagataaaa tttcgctgtt gatatggcta ttttttattt gtggagctaa
    16381 ggagaaaata tcgctcttta tgagcatctg ggcatagctt cctaaggatg gttgtttaca
    16441 cttatcatta ttggaattct cagtatttac atagtcctta aaatattgct ggagtgtttt
    16501 ttcttcttcc ccatttcttt tttcaacccc gaaacctcgg gcgaagccaa caaggtaatc
    16561 ttcagtgtat tccttgtcat aaaaagaaat cttttttgag ttgttgatgt tttcgaattg
    16621 gtgtgtttta gagtatagtg cgggaacttt gagagagttc tgagagctac cattcgtcgg
    16681 ttttagaaga taagtttctt gtttccagta ttcgctgctt ttatctctaa gtttttttaa
    16741 gcgatcaatt tgctcatttg ttaatttaga attgtcgtaa acgtaatcaa cagccaaaag
    16801 cggagaggta taaagaatag aaaaaaatag acttacaata aatgattttt ttaaacttct
    16861 gcttgcttcg agtttcataa taaattttcc tttgtcaagc aaaaataaat ggggcgtgga
    16921 ttttagcata aaactgaaca aaaaatgtca tttagctcac atttttctct atttatttct
    16981 tatttattaa aggtaaacgt ttgctttttg ctattttgtc aagccagttt gaaaatgtgt
    17041 ataattgccc tcgtttttta caaaaatttc aggaaaaatg accgcacttt acccttggct
    17101 aatgccaatt tatcatcaaa ttgctcaaac ctttgacgaa ggtttggggc atcatgctgt
    17161 gctgattaaa gcggatgctg gtttaggtgt agaacgttta cacatcaggc ggcagccttg
    17221 cccataccct ctgaagcact gtttccacaa tcagcgcgta tgcttaatca accgctgttt
    17281 ctcgcgtttc caatccgcct ctttcatact ctgacgtttg tcgtgctgtt tcttaccttt
    17341 tgccaaaccg atttccatct tgatttttcc gcgcgaaaaa tgcaaatcca gcggcacgat
    17401 ggtgtagccg gcacgttcgg ttttgccgat taatttgttg atttccgact ggttcaacaa
    17461 gagcttgcgc ggacgtacgg catctggttt aatgtgtgtc gaggctgtgg gcaaagccgt
    17521 aatatggcag ccgaccagat aaaacgcgtc ttttttccaa tagatataac tctctttaag
    17581 ctgtacgcgc gcggcgcgga ttgctttgac ttcccagcct tccaagacca aaccggcttc
    17641 aatccggtct tcaatgaaaa aatcgtgaaa tgctttttta ttgttcgcaa tagccataaa
    17701 catcctatca atatccgccg tcagacggca taaacccgaa aacagaaccc atcataccgc
    17761 ctcttcaacc gcctgcacaa tcttctcggg atacagcctg ttgaggcagt cggtatgccc
    17821 cagcggacat tcccgcttaa aacagggcga acattccaag tgcaggctga cgattttcgc
    17881 cctgtcgctc aaaggcggcg tatgcgtcgg gctggaagaa ccgtaaaccg ccaccacctt
    17941 cctgcccaat gccgccgcca agtgcatcag cccgctgtcg ttgcacacaa ccgtgtccgc
    18001 caacgacagc aaatccattg cctgcgacaa atcggttttg ccgcacaaat tgacacacat
    18061 accgtctgaa aggcagttga tttcctcggc aatttcatca tctttttgcg aaccgaacag
    18121 ccaaacctgc caacccgccg ccagataatg tttgcccaac tcggcaaaat gccttgtcgg
    18181 ccaacgcttt gccggcccga attccgcgcc cgggcaaaat gccaaaacag gctttccaag
    18241 attcaaaccg aaagtttcga cagaaatttc ccgccgccgt tcatcaatgg aaaactcggg
    18301 gaatcccgaa tgcccgtcaa aatcttcctg actcggatgc gcgagagccg tatatctgtc
    18361 caccatcaaa ggcagacgtt ccttatccag cctgcgtata tcgttcaaca gaaaataacg
    18421 gctttcaccg acataacccg tccttttggc aatacccgtt gctagcgcga tgactgccga
    18481 tttcaaagaa ccgggcaaca cgataacctg atcgtatccg cgccgcccca aatccctgcc
    18541 gacccgccag cggcgtttca actccaacgc accgtgtccg aacggattct caagaatttc
    18601 attcacttcc ggcatacgct cgaacaccgc catcgaccac ttcggcgcga acacatcaat
    18661 cgtgcaaccg gggtgaagtt ccttcaaacg gcggaacaag ggctgggtca tcacgcagtc
    18721 gcctatccaa ctgggagaaa taatcaggat tttgatggac ataacaagaa accgaaatca
    18781 gacaggcaga attttaccgc gaaaccgttg gaaaacctat cttgtctcat tccgaacgcc
    18841 gtacgtgcaa atatgaaaaa gcccgaacat tcaagttcgg gcttcaaaat tctggctccc
    18901 cgacctgggc tcgaaccagg gacctgcgga ttaacagtcc gtcgctctac cgactgagct
    18961 atcggggaat ggggcgtatt atagcgtccg gaaaaaatgt gtcaatcctt aattttggaa
    19021 aaatgggcga caaaacgaca agcatatgaa tcagaaagac attaagaccg atgccttaaa
    19081 aggattgccg ttgtatgaat ttccacagcc gtcatcacac catatttaag cccgatgagc
    19141 cgttctgccc tcccccgctt aaaacaatgc cgtctgaact tcgccgcgtt ccaaagccag
    19201 taaaaactgt ttgcggttca accctcccgc gtagccggtc agtttgccgt cgctgccgat
    19261 gacgcggtgg cagggaatca agatagacac cttgttctgc ccgttggcgg cggcaacggc
    19321 gcggacggcg ttggggttgc ccaaacgctg cgcctgctct ttgtagctgc gcgtttcgcc
    19381 gtaaggaatc gccaagagcg cgtcccatgc ctgcttttga aactcggtgc caatctgctc
    19441 caaaggcgtg gcaaaggttt tcagacgacc cttgaagtat aagtccaatt cctgccgcaa
    19501 aagttgcgtc cgctcatcct cccgaaacac aaaccgtccg cgcaaggctt tttggacggc
    19561 ggcaatttcc tgttccaaat gcttctgtcc gacaaactcc agcagacaca agcctttact
    19621 gccgaacacc gccagcatct cgcccaaagg cgtggcaatg gcggcacaca ccagctcgtt
    19681 caaactgtcg ggataactcg cttccagcag acggatggcg cggcggatac ggacatattc
    19741 ttcaggcgca cagccgatat tgtcccaaaa atcccgctcg aactgcccgg cttcgcattc
    19801 cgtcaaattg ggatgcggca taacgccgca ctcacaaacc cgggtttcga gccaatagcg
    19861 gatttcatcc cattttgacg gcagattgtt taaggaaggc agggtaatca ttcgtttgct
    19921 ccgtatctct atcatggatt tggcggcaaa atccccaatt tttgccattc ccgcacgccg
    19981 gagcaggaac gggctatgac gtaaatcttg agggttaggt tgcggcaatg cctaaatatg
    20041 cgatatttct aaagcctcag ggaaaggaat taaagacttc agacgacctg caaagggtcg
    20101 tctgaaacca cgatttttgc atttacgcat tccggcacat catccaaccg tttcggcaca
    20161 ttcctgccgc cgttgacagc ctataatgaa tccacttatt catcaagcaa aggaatcatc
    20221 tatgcaaacc cgcatcctct ccgccgtact gctggctttt tcaaccgctg cctttgccgg
    20281 gggcgcattc acgctgcaat tcgacaaccc gtccgaagac ggcggcttca cgcaaaacca
    20341 gcttttgagt gcgccttacg gcttcggctg ttcaggcgac aatgctccgc ccgcgctgtc
    20401 gtggaaaaat ccgcccgccg ggacaaaaag tttcgtcctg accgtttacg ataaagacgc
    20461 gccgaccgga ctgggctgga tgcactgggt ggtcgccgac attcccgccg atgtccgccg
    20521 ccgcaacgcg gcctcgctgc aattaagccg ctgcgccaac atcgccgacg accagcccgc
    20581 agccatatcg gcagtaatca gtttgcggat ttgccgcatc aggttgacgc cttcgtacac
    20641 ggcaaaaccg atgccgtcat gctgcaacca cgccaacacg ccgcaaagcg cggcctccgc
    20701 cacattgtgc ggcacttttt catccgccag caccgcagcc gcataatcaa acgccgcgtt
    20761 catctgcctg ggatgcggca gcttcaccgc atcgcacact gcctgcgccg tcccgtattg
    20821 tgcggcaaac ctttctatgg cttcctgttc gaaagcaatc cattgcgcct gatagaggtc
    20881 gtctgaatcg ggaatattga tgacgtcaaa cgtctgcccg cctgccaacg caaccgcctc
    20941 acccgccgca atcctcaatt cgcgcctcgc tcaccgcagc ttcttgcttc cgcgccgcac
    21001 gataagcaca gccggttcat ataccgccac gctgcggtac aaggcggtat gatgttgcac
    21061 gatgccgccc aaagcaccca atcgttcgcg cgtatgaaag taatttgacg gcactattat
    21121 ctccaacacg cagggccgca cataaggcgc agccctgttt accctgattt ggaaagggtt
    21181 acttcccaaa aaataaacca aaatcccacc gccccgaatg gccgggtttc aaccatattc
    21241 cgtcggcgtg cctgaaactt gacccctccc ctgtcgggga gggtcgggga gagggtaaaa
    21301 cggggcagat acaggcaata tttccgttgc cgccccgatg ccctctccct aaccctctcc
    21361 cacgaaagag ggaatggatt gccgtttaaa taaaaaaatc tacataaaaa tcaatgtttt
    21421 atagtggatt aaatttaaat caggacaagg cgacgaagcc gcagacagta caaatagtac
    21481 ggcaaggcga ggcaacgccg tactggtttg aatttaatcc actatatctc aaacccacat
    21541 taggtctaat caaatggtcg gacatccata tccgacagcc cattttcttt tcagacggca
    21601 tttccagcca acaagcgcgc caatatcccc tcatacaccg ccgacagctt cggaatatcg
    21661 tccagccgca cgttttcgtt gatttggtgg atggtcgcat tggacgggcc taattcgatg
    21721 agttcttttg caatggcttt gatgaagcgt ccgtccgaag tgccgccggt ggtggacaat
    21781 tcggcctcaa tgccgcaggt ttcggcaatg gctgcgcgtg ccacgtcggt cagtttgccc
    21841 gcttgggtca gaaagggctg ccccgaacac gaccactgta aatcgtattg cacgccgtgt
    21901 ttgtccaaaa tggcgtgaac acgttgtttc agccctgctt cggtggactc ggtggagaag
    21961 cggaaattga atttgacgct cagctcgccc ggaatgacat tggtcgcgcc tgtaccgccg
    22021 ttgatattgg aaatttgaaa gctggtcggc gggaagtatt tgttgccttc gtcccagact
    22081 tcctgcgtca gctctaacaa ggccggggca aaagtatgca cgggattgat tgccaaatgc
    22141 ggataggcaa tatggccttg cttgcctttg acggtcaggt tgcccgacag cgagccgcgc
    22201 cggccgtttt taatcatatc gcccaatttg tccacggctg tcggttcgcc gacgatgcag
    22261 tagtcgataa gctcgtcgcg cgctttcaat acatcgacga ctttggtcgt gccgtccaac
    22321 gcgtcgccct cttcgtcgga agtaatcaga agcgcaatgc tgccttggtg gtcgggatgt
    22381 tcggcaacga agcgttcaca ggcggtaacg aagcaggcga tgctggtttt catgtctgcc
    22441 gcgccgcgcc cgtataatct tccgtcgcgc tcggccggtt cgaacggggg cgaatcccat
    22501 ttttcgacag gacctgtcgg tacaacgtcg gtatgccctg caaaacagac gacgggagct
    22561 ttcgtgccgc gtcgcaacca gatgtttttg gtgtcgccga aatggagtgc ttcagccgca
    22621 aaaccgattt tgtgcaggcg ttcggcaagg agtttttggc aatccctgtc gtcaggggta
    22681 acggatggtc gggaaatcag ctctttggca agctctaggg attgagtttc ggtcatattt
    22741 gttcactttt gaaattagac cgtctgaaac gttctgaatg tgattttcag acggcattta
    22801 ggttaggttg gcatacgggg tgggtatttt acccatcagt cttctgaatc atttgccgtg
    22861 acacgcttca taaagcgaca gcaagtcttc cactgtttcc gctatccatt tcacgacatc
    22921 ctgcctgccc aaatcgtcgc gctcgatgtg tttgccgatg cagaagaaat cttcgtcgtt
    22981 ttgcaacctt cggtcggatt cattttgctg cgcgacagtg cggtagtcgt cgtattcgct
    23041 ttccgcgccg tgccacatat cgaaagaagc gtatttttca gtatcaaaat tatccaacca
    23101 acggttgtaa tcaggcagcg caatcggcga cacgtcggct ttgtagcaat gccagtccaa
    23161 gctgacactc aaacggcggc ggttgagcag tatcgacaaa atcgcggcgg aatttttata
    23221 ttgttcgtat ttgaagtagg caaagaaatg ggcgcgaacc tgccagccgt tgcaccagcg
    23281 ttcgatgtgc ggcggcgcaa acggcgcacc caattcggcg gcaacctgct gaatcagctg
    23341 ctgccatatc tgccagtttt ccttgtagtc ggctttgatt tgcggaatgc tttcaggctg
    23401 gtatttttta agctgggaaa attggaaaaa cgggatattg aacaaatcgc aacttttcgg
    23461 ggtcagcata atatatcctt gagacgattg tttcagacgg cattatctgc gccggcgcgc
    23521 cgccatgatt tcgccgattt cggtcagttt ctctttgggg atgaacatct tgcccatgtc
    23581 gaacaagggt tcttcaatcg ccaaatgcac atcatatccc gccacaaagc gtttgaacgc
    23641 ttccgcatcg ggaatatagg cattgtctgc ttcgagtttg gcaaattcgg cagccacggc
    23701 atcccagttg ccatgcaggc ttacatgttg gcgtaaaagc tcgtccacgc tttcttgggc
    23761 ttgcggcgca tattgcagca ggagcgggaa aaagttttct tcttcgtcct catggtgcag
    23821 cggcgcggca acgttgaaat accgggtgat ttggcggatg gtttgcaaaa caagctgatt
    23881 gcagccgttt tcggcgatgt agtccgacag catcgcgact tggccgcaaa aacggcgtac
    23941 tttgccgtgg caggcataca gcatttcaat cggttcggca aaggtaacgc tttgggtttc
    24001 aaacggattc atgttttcgt tctcaacggg cacttttcaa gcagtcattt tataataaaa
    24061 cagcctgcac aaagcaggct gtccgtcttt tgagacttta agcggattaa tcgaccaaag
    24121 tcactttgcc gttcatcaaa gcaccgtgac ccgggaaggt gcaagcgaat ttgtattcgc
    24181 cgtcagccag tttggcagga tccagagtca aagaagattc ttcgccgcca ccgatcagtt
    24241 tggtgtgggc aacaacgcgc gcatcgtcag gtttaacgta gtcagtgtca gctgcgccaa
    24301 cgccatcttt gaaaataccg tccatgtctt cagttttacc gatgacaatg ttgtgaccca
    24361 tgctggtttt aggttgggta ccggtgtgtt tcagggtgat ggtgaattcc ttacaagctt
    24421 tgcttacttg gatgtcttta gtgttgaact gcatattgtc gttggattcg acagttgccg
    24481 cacagttgcc ggcagcaggg gcttcggcag catctgcagg agcagcttcg gcggcaggcg
    24541 cttcggaagc gggtgcttca gcagcaggag ttgcctcggc agcaggcgcg gcaggttctt
    24601 gagagcaggc agccaaaccg ataacggcgg cagaaatcag agccagatac gctttcataa
    24661 caaatctcca atcgataaaa taatattcgg ttttacagaa atcaaagtgc aaccgccatt
    24721 aacaaaacct tgaaaaagat tccgccgcgt tgcacaaaca gatgtttcgg agcggcattt
    24781 tgctacaaat ttcatttgaa atcaaagcct gtctgcaagt ttacaatcgt ttactttaaa
    24841 aggggcaatt ttaccccgaa cctatttctt tagtattaga cccattatcc tttacttctt
    24901 aatattaacg gatgtttaca caaattcccg tatacatttt atgcgccatg ccttctaacc
    24961 aagtttgcca atgcctccgc caattcggga tgccgttttt ccaactttgc cgccgccgaa
    25021 ccgaaactct ccagcgcagc cttactcaaa tgcagggtat tggttttcgg cggtttttcc
    25081 ggtttcggga ccagcctgac cgaaacagag cgtatcgaag cgtcaagccc tgccaactgc
    25141 ggcaataccg acggtgcaat cattttcaag cgcgatgccg ccatattgtt tgccgccaaa
    25201 aggacaagcc tgccgtcttc gatacatgcc gtctgaaaat gcgggtgcag gttggcaggc
    25261 agcagttttt tcacggcggc atccaaccgc cgccactgtc ccgcctgttt caaaagtccg
    25321 gaaagcagcg cgtcccgcct gcccaactgt tccaaattca taaaacatac acccaaaaag
    25381 attgaaatac cgcaaacgcg cctttatttc agacggcatt agcactttgc acaaacgctt
    25441 gtgttaaaat cgcgttttcg cccactatta tatcaggcgc aggaattatt catgctgaca
    25501 aacattgcca agaaaatctt cggcagccgc aacgaccgct tgctgaaaca ataccgtaaa
    25561 tccgttgcca gaatcaacgc gctcgaagaa cagatgcaag ccctaagcga tgctgatctg
    25621 caagccaaaa ctgctgaatt caaacaacgc ctcgccgacg gtcagacttt ggacggcatt
    25681 ttgcccgaag ccttcgccgt ctgccgcgaa gcgtcccgcc gcaccctcgg tatgcgccac
    25741 ttcgacgtgc agcttatcgg cggtatggtg ctgcacgacg gcaaaatcgc cgaaatgcgt
    25801 accggcgaag gcaaaaccct ggtcgccacc ctcgccgtct atctcaacgc gctggccggc
    25861 aaaggcgtac acgtcgttac cgtcaacgac tacctcgcct cgcgcgacgc aggcattatg
    25921 gagccgctct acaatttcct cggcctgacc gtcggcgtga ttatttcaga tatgcaaccg
    25981 ttcgaccgtc aaaacgccta tgccgccgat atcacctacg gcaccaataa tgaattcggc
    26041 ttcgactacc tgcgcgacaa tatggttacc gaccaatacg acaaagtgca gcgcgaactg
    26101 aatttcgccg tcgttgacga agtggactcc atcttgattg acgaagcgcg cactccgctg
    26161 attatctccg gccaggcaga cgacaacatt cagttgtacc aaatcatgaa caccgttccg
    26221 ccccacctcg tccgccaaga aaccgaagaa ggcgaaggcg actactgggt agatgaaaag
    26281 gcgcaccagg tcatcctgag cgaagcaggt catgaacacg ccgagcaaat cctgacccaa
    26341 atgggattgc tggcagaaaa cgactccctc tattccgccg ccaatatcgc cctgatgcac
    26401 caccttatgg cggcattgcg cgcgcattcc ctcttccaca aagaccaaca ttacgtcatc
    26461 caagacggcg aaatcgtcat cgtggacgaa ttcaccggcc ggctgatgtc cggccgccgc
    26521 tggtcggagg gtctgcatca agccgtcgaa gccaaagaag gcgtggaaat caaacgcgaa
    26581 aaccaaaccc tcgcctccat caccttccaa aactatttcc gcctgtacac caagctctcc
    26641 ggtatgaccg gtacggcaga taccgaggct ttcgagttcc aaagcattta taacctcgaa
    26701 accgtcatta tcccgaccaa ccgccccgta cagcgcaaag acttcaacga ccagattttc
    26761 cgttccgccg aagaaaaatt cgaagccgtc gtcaaagaca tcgaagaatg ccacaaacgt
    26821 gggcagcccg tcctcgtcgg caccaccagc atcgaaaact ccgaactggt ctcccacctt
    26881 ctgcaaaaag ccggactgcc gcacaacgtc ctcaacgcca aagaacacga acgcgaagcc
    26941 ctgattgtcg cccaagccgg caaagtcggc gcgattaccg ttgccaccaa tatggcggga
    27001 cgcggtacgg acatcgtttt aggcggcaac ctgaagcacc aaaccgatgc catccgtgcc
    27061 gacgaaacct tgagcgacga agagaaacag gcacaaatcg ccgcactcga aaacggctgg
    27121 caggcagaac acgataaagt aatggaagca ggcggcctgc acatcatcgg tacggaacgc
    27181 cacgaaagcc gccgtatcga caaccaattg cgcggacgtt ccggccgtca gggcgacccc
    27241 ggatccagcc gtttctacct ttcgtttgaa gacccgctgc tgcgcttatt cgcactcgac
    27301 cgcgccgccg ccatcctcaa ccgtctcgcc cccgaacgcg gcgttgccat cgaacacaac
    27361 ctgctgacgc gccaaatcga aggggcgcaa cgcaaagtcg aaggcagaaa cttcgatatg
    27421 cgcaaacagg ttttggaata cgacgacgtt gccaacgaac agcgcaaagt catttacagc
    27481 cagcgcaacg aaattctgac cagcaaagac atcagcgacc tgatgcagga aatccgttct
    27541 gatgtcgtca gcgacctcgt ggatacctat atgcctcccg acagcatgga agaacaatgg
    27601 gacatcccga ctttggagaa ccgtctggct gccgaattca gactgcacga agacatccaa
    27661 tcctggctga aggcggacaa tgcgattgac ggtcaagaca tcaaagaacg cctgatcgaa
    27721 cgcatcgaaa acgaatatgc cgccaaaacc gaactggtcg gtaagcaggc aatggccgat
    27781 ttcgagcgca acgtgatgtt gcaggtcatc gacaaccaat ggcgcgaaca cctcgccgct
    27841 atggactacc tgcgacaagg catacacctg cgcagctatg cccaaaaaaa tccgaagcag
    27901 gaatataaac gtgaagcctt taccatgttc caagacctgt ggaacggcat caaattccat
    27961 attgcctccc tgcttacctc ggttcaaatc gaacaaaacc ctgtcgcggt ggttgaagag
    28021 caacccatcg gcaacatcca gtccatccat tccgaatcgc ccgatatgga agaacttttg
    28081 ggtcagtcgc aaaccgattt ggttaccgag gcctttaatc ccgatggaac ggatttcagc
    28141 cccgaagcct tggaagcgcg gggacaaatc gtccaccgca acgacccctg cccctgcggc
    28201 agcggtttga aatacaaaca atgccacggc aaactggctt aagcgtttga acgcaaatgc
    28261 cgtctgaaca tccgatttcc ggtttcagac ggcattttgc ctgaaccgcc acatccgact
    28321 gccattccga aaaatcccga tttcgtaccg tccgtaccaa aaacagacat cccgtccgcc
    28381 ccacatcatg attccatccg acttcattga cgagctttta gccaaaaccg atattgtcga
    28441 tattatcgac gagcaggttc cgctgaaaaa aggcggggcg aactatatgg cgtgttgccc
    28501 gttccacaag gaaaaaacgc cgtcgttttc ggtcagtccg accaaacagt tttaccactg
    28561 tttcagttgc ggggcgcacg gttcggcgat tggttttgtg atggaacatc aggggctgtc
    28621 gtttccggag gcggtgcagt tccttgccga ccgcgtgggt atgattgtgc ctaaagtgcg
    28681 cgggcaaaac gataatcccg aagtccgtgc cgagcgcaag aaaaaacagc agacgctgga
    28741 ggaaacgacg gctgcggcag ctgattttta tgcgcaacag ctaaaattca atccggcggc
    28801 aaaagcttat ttggacaagc gcggcttgag tgcagaagtt atcgcgcatt atggtttggg
    28861 ctacgcgccc gacggctggc agcctttggc gcaagtgttc caaccgtatc cgaataccgc
    28921 gttggtggat acggggatgg tgattgacaa tgaggggcgg tattacgacc gcttccgcca
    28981 tcggattatg ttccctatcc gcaatccgcg cgggcaggtc atcggtttcg gcggcagggt
    29041 gctggacgac tccaaaccca aatatctaaa ctctcccgat acacctttgt ttgacaaagg
    29101 gaaaaacctt tacggcttgt atgaggggcg tgccgctgtc aaggaagcgg agcggattct
    29161 ggtggtcgaa ggctatatgg acgtggtcgc gctggcacag ttcggcgtgg gctacggcgt
    29221 ggcggccttg ggcacggcaa caacagcgga acacgtcaaa atcctgatgc gtcaggcgga
    29281 cagtatttat ttctgtttcg acggcgacag cgcggggcgc aaagcggctt ggcgcgcgct
    29341 ggaaaacgcg ttgccgcagt tgaaagacga caaatcgctg cattttttgt tcctgcccga
    29401 ggaacacgac cccgacagtt acatccgtgc ctacggcaaa gcgcaatttg aagacgcgct
    29461 tctgaatcaa agcaagcctt tgtcggagta tttctgggaa cacctttcag acggcattca
    29521 tctcaatacg caggaaggca aggcggaatt ggtgaaaacc agttcgccgc ttttggcgca
    29581 gattaccgcg ccggcattgg cttatttgtt aaaacaacgg cttagcgaac tggtcggcat
    29641 cgaccccgac aacctcgcgc aactgctagg acaggaagcg ccgaagcggc acgtcaaaca
    29701 aaaaaactac aaactgcctc cgatttccgt caaacagccc gtcatgctga cgctggtaca
    29761 gcggcaaatc cgcagcctct tgataaatcc ggattgggct gcatatatag acctgcccga
    29821 ttatctggcg ttggacggtg atttcgcctg ccttgccaat cttgccgaat cgattaaaaa
    29881 ccatgccgcc gtacccgaaa ccgctcaggt tttagagtat atgcgcggct cgccttacga
    29941 agaaacgata acccgaatct tccattcaac gcaccaatcg gaagaaatga acagcagcag
    30001 tgaagaagat tgcgagaatt tccaaatcgg catgaaaaaa ctgctcaatg agttaaaata
    30061 cagccaaatc gaagcattga aacaaaaaag cctgcaatcc ggcttaaatg aaagcgagaa
    30121 aaaacttttg ctgtcgttgc tgaccgcaaa acaaaattga ccggcggatt ccgccacccg
    30181 taaacccgtt atgccgtctg aaaagcactt accccggctg caacaacgac acctgcagaa
    30241 cacccatccc aaaaagcctt cagacggcat cagagtaccc gctctgccac gccttcaggt
    30301 gcgttcaaac gcaaaccgtc ggcaccttac caacagaaag cagacaatgt ccagaaacca
    30361 aaatcacgaa gaatatcaag acgacacccg tccgttaagc attgaagagc aacgcgcgcg
    30421 cctgcgtcag ctcatcatca tgggtaaaga acgcggctac atcacctact ccgaaatcaa
    30481 cgacgccctg ccagacgata tgtctgatgc cgaccaaata gacaatatcg tcagcatgat
    30541 ttccggtttg ggcatccaag ttaccgaaca cgcccccgat gcggaagaca tattgttaag
    30601 cgacaatgcc gccgttaccg acgatgatgc cgtcgaagaa gccgaggccg ccctttccag
    30661 tgcagattcc gagttcggca gaaccaccga ccccgtccgt atgtatatgc gcgaaatggg
    30721 gcaggtcgac ctgctgaccc gcgaagacga aatcatcatc gcaaaaaaaa ttgaaaacgc
    30781 cctgaaaaat atggttcagg ccatctccgc ctgcccggga tccattgcgg aaatcttaga
    30841 actcatcgaa aaaatccgca aagacgaaat ccgcgtcgac gaagtcgtag aagccattat
    30901 cgacccgaat gaagtattgc tcaacgaatt gggcttgggg cacttggaaa ccacagcgcc
    30961 cgagaaacct tccaacgaca attcggacga aaacgaagac gacgaagaat cggaagaaga
    31021 tgcggatgaa atctcggcag ccaatctcgc cgaattgaaa caaaaagtca tcggccactt
    31081 tgcccaaatc gaaaaagact acaaaaaaat gattggccgt ttggaaaaac accacagccg
    31141 gcacaaagac tatctcgcct accgcgacgc gattgccaac aaactgctgg aagtccgttt
    31201 cgccacccgg caaatcgaca gcctcagcag cagcctgcgc gggaaagtag aaaacatccg
    31261 caaactcgaa cgcgaaatcc gcgacatctg cctcgaccgc gtccatatgg aacgcgacta
    31321 cttcatccaa aacttcctgc ccgaaatcac caatctagaa tggattgaag aagaaatcgc
    31381 caaaggcagg gtttggagcg acgcgctcga ccgcttccgc cacgccatcc tcgaaaaaca
    31441 aaccgagttg gcggatatgg aaaaagaaac ccgcatttcc atcgaagagt tgaaagaaat
    31501 caacaaaaat atggtgtcga gcgaaaaaga aaccgcagcc gccaaacagg aaatgattca
    31561 ggcaaacttg cgcctcgtga tttccatcgc caaaaaatat accaaccggg gcttacaatt
    31621 ccttgatctg attcaggaag gcaacatcgg tttgatgaaa gcggtcgata agttcgaata
    31681 ccgcagaggc tataaattct ccacctacgc aacctggtgg atccgccagg caattacacg
    31741 ctcgattgcc gatcaggcgc gtaccatccg cattccggta catatgattg aaaccatcaa
    31801 caagatgaac cgcatctcgc gccaacacct tcaagaaacc ggcgaagaac ccgattccgc
    31861 caaacttgcc gaactgatgc agatgcccga agacaaaatc cgcaaaatca tgaaaatcgc
    31921 caaagagccg atttcgatgg aaacccccat cggcgacgac gacgattcgc acttgggcga
    31981 cttcatcgaa gatgccaaca atgttgcgcc ggccgatgcg gcaatgtaca ccagcctgca
    32041 cgaagtaacc aaagaaatcc tcgaaagcct gacaccgcgt gaggcaaaag tcctgcgtat
    32101 gcgtttcggc atcgatatga acaccgacca cacgctggaa gaagtcggca gacagtttga
    32161 cgtaacgcgc gaacgcatcc gacaaatcga ggcaaaagca ctccgcaagc tgcggcatcc
    32221 gacaagaagc gaccgtttga gaagtttctt ggacagcgaa gacagcaagc tgtaaaccaa
    32281 aaaaccgcag gtttcaaata cctgcggttt tttcttacac aataaacaac gcttccacat
    32341 atcccacact cctatcccga gacctttgca aaat
//
LOCUS       DAXHTY010000012        31826 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXHTY010000012 DAXHTY010000000
VERSION     DAXHTY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31826
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.012"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(187..462)
                     /locus_tag="ACFNMK_000478"
     CDS             complement(187..462)
                     /locus_tag="ACFNMK_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6363807.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(459..1742)
                     /locus_tag="ACFNMK_000479"
     CDS             complement(459..1742)
                     /locus_tag="ACFNMK_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6363808.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(1898..3316)
                     /gene="glnA"
                     /locus_tag="ACFNMK_000480"
     CDS             complement(1898..3316)
                     /gene="glnA"
                     /locus_tag="ACFNMK_000480"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC6363809.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDAGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3627..4436
                     /gene="aroE"
                     /locus_tag="ACFNMK_000481"
     CDS             3627..4436
                     /gene="aroE"
                     /locus_tag="ACFNMK_000481"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC6363810.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFVQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLILLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKGHRPARIVVANRTHAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPEIFLGCRLAYDMVYGDA
                     AQEFLNFAQSNGAAEVSDGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            4438..5139
                     /gene="mtgA"
                     /locus_tag="ACFNMK_000482"
     CDS             4438..5139
                     /gene="mtgA"
                     /locus_tag="ACFNMK_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC6363811.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHQTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(5464..5539)
                     /locus_tag="ACFNMK_000483"
     tRNA            complement(5464..5539)
                     /locus_tag="ACFNMK_000483"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5504..5506),aa:Phe,seq:gaa)
     gene            complement(5730..6464)
                     /gene="lptB"
                     /locus_tag="ACFNMK_000484"
     CDS             complement(5730..6464)
                     /gene="lptB"
                     /locus_tag="ACFNMK_000484"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC6363812.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(6538..7068)
                     /gene="lptA"
                     /locus_tag="ACFNMK_000485"
     CDS             complement(6538..7068)
                     /gene="lptA"
                     /locus_tag="ACFNMK_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC6363813.1"
                     /translation="MIQKICKLFVLIEFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7049..7630)
                     /gene="lptC"
                     /locus_tag="ACFNMK_000486"
     CDS             complement(7049..7630)
                     /gene="lptC"
                     /locus_tag="ACFNMK_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC6363814.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(7627..8163)
                     /locus_tag="ACFNMK_000487"
     CDS             complement(7627..8163)
                     /locus_tag="ACFNMK_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC6363815.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(8381..9355)
                     /locus_tag="ACFNMK_000488"
     CDS             complement(8381..9355)
                     /locus_tag="ACFNMK_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC6363816.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKNITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQCRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            9451..10506
                     /gene="tal"
                     /locus_tag="ACFNMK_000489"
     CDS             9451..10506
                     /gene="tal"
                     /locus_tag="ACFNMK_000489"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC6363817.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQNLSPKQRYETMAVADVRAACGICLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            10521..10655
                     /locus_tag="ACFNMK_000490"
     CDS             10521..10655
                     /locus_tag="ACFNMK_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363818.1"
                     /translation="MPSERLSDGIARLTGHSAAYQGFTLKILMGKKLMHDITHQHKRK
                     "
     gene            10660..10951
                     /locus_tag="ACFNMK_000491"
                     /pseudo
     CDS             10660..10951
                     /locus_tag="ACFNMK_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10968..11855
                     /gene="gluQRS"
                     /locus_tag="ACFNMK_000492"
     CDS             10968..11855
                     /gene="gluQRS"
                     /locus_tag="ACFNMK_000492"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC6363819.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAAAVHGADGFVYNGRCRNPQQRPAPQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITTP"
     gene            complement(11923..12933)
                     /gene="dusA"
                     /locus_tag="ACFNMK_000493"
     CDS             complement(11923..12933)
                     /gene="dusA"
                     /locus_tag="ACFNMK_000493"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC6363820.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYLARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQCLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDAMLLKDNDGGLILEAWEEVERANT
                     RE"
     gene            complement(12964..13455)
                     /gene="dtd"
                     /locus_tag="ACFNMK_000494"
     CDS             complement(12964..13455)
                     /gene="dtd"
                     /locus_tag="ACFNMK_000494"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC6363821.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDIEK
                     DARYVADKIAHLRVFEDEAGKLNLSLKDVGGSVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(13529..14287)
                     /locus_tag="ACFNMK_000495"
     CDS             complement(13529..14287)
                     /locus_tag="ACFNMK_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6363822.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(14358..15224)
                     /locus_tag="ACFNMK_000496"
     CDS             complement(14358..15224)
                     /locus_tag="ACFNMK_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6363823.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(15273..15551)
                     /locus_tag="ACFNMK_000497"
     CDS             complement(15273..15551)
                     /locus_tag="ACFNMK_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6363824.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(15551..15841)
                     /locus_tag="ACFNMK_000498"
     CDS             complement(15551..15841)
                     /locus_tag="ACFNMK_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC6363825.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(15844..16374)
                     /locus_tag="ACFNMK_000499"
     CDS             complement(15844..16374)
                     /locus_tag="ACFNMK_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC6363826.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVIFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            16683..19235
                     /gene="tspA"
                     /locus_tag="ACFNMK_000500"
     CDS             16683..19235
                     /gene="tspA"
                     /locus_tag="ACFNMK_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
                     /protein_id="HFC6363827.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENVTETV
                     AETPDFNATADDLSALLQPSEVPAVEENAAEIVADDLSALLQPAEAPAVEENVTETVA
                     ETSDFHTAADDLSALLQPAEAPAVEENVTKTVAEIPDFNATADDLSALLQPSEVPAVE
                     ENAAEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTGFPSESVG
                     SDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(19408..19824)
                     /gene="gloA"
                     /locus_tag="ACFNMK_000501"
     CDS             complement(19408..19824)
                     /gene="gloA"
                     /locus_tag="ACFNMK_000501"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC6363828.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(19848..21017)
                     /gene="lapB"
                     /locus_tag="ACFNMK_000502"
     CDS             complement(19848..21017)
                     /gene="lapB"
                     /locus_tag="ACFNMK_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC6363829.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(21028..21342)
                     /locus_tag="ACFNMK_000503"
     CDS             complement(21028..21342)
                     /locus_tag="ACFNMK_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC6363830.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            21652..22650
                     /gene="ilvE"
                     /locus_tag="ACFNMK_000504"
     CDS             21652..22650
                     /gene="ilvE"
                     /locus_tag="ACFNMK_000504"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC6363831.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLVPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(22894..23679)
                     /gene="fabI"
                     /locus_tag="ACFNMK_000505"
     CDS             complement(22894..23679)
                     /gene="fabI"
                     /locus_tag="ACFNMK_000505"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC6363832.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFVDLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(23834..24655)
                     /gene="dapD"
                     /locus_tag="ACFNMK_000506"
     CDS             complement(23834..24655)
                     /gene="dapD"
                     /locus_tag="ACFNMK_000506"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC6363833.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPTGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(24865..26508)
                     /gene="pgi"
                     /locus_tag="ACFNMK_000507"
     CDS             complement(24865..26508)
                     /gene="pgi"
                     /locus_tag="ACFNMK_000507"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6363834.1"
                     /translation="MNAFTRAWHALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYVDGRDVLPEIRRELNRALEFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSSVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            27018..28250
                     /gene="pilG"
                     /locus_tag="ACFNMK_000508"
     CDS             27018..28250
                     /gene="pilG"
                     /locus_tag="ACFNMK_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC6363835.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            28324..29184
                     /locus_tag="ACFNMK_000509"
     CDS             28324..29184
                     /locus_tag="ACFNMK_000509"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC6363836.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRARQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLS
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSLWLLCAVYKLLTRKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            29186..29818
                     /gene="coaE"
                     /locus_tag="ACFNMK_000510"
     CDS             29186..29818
                     /gene="coaE"
                     /locus_tag="ACFNMK_000510"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC6363837.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FNDAPYGIIEIPLLTEKRQFMKLIQRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLRDKTMRLHAFYSGIFASKPTQGKHNG"
     gene            29811..30020
                     /locus_tag="ACFNMK_000511"
     CDS             29811..30020
                     /locus_tag="ACFNMK_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC6363838.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSSQTESLPEISEPDMAYR"
     gene            complement(30105..31781)
                     /gene="pilB"
                     /locus_tag="ACFNMK_000512"
     CDS             complement(30105..31781)
                     /gene="pilB"
                     /locus_tag="ACFNMK_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC6363839.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         7435 a         8993 c         8185 g         7213 t
ORIGIN      
        1 caaatccaac aaacagttca tcgtattctt ttctgttggc gtataaaaaa tactgatata
       61 tgccttcact ttcatagggc gacgctttat gtgccgtcct gtacgttgaa acatcggttt
      121 tccttgcccc ctgctttgtc ccgattgttg ccaactgctg taaccatacc gtccgaacct
      181 aaaccgtcag gtttcagacg gcattttttc tgcctgctcc cacaattgcc gtttttgtgc
      241 ggcggtcagt tttttgacgg cgatttcctg cctgagtgcc gtgcctttat ccatcccgcc
      301 tgcaacgata cgcatcgcca ccggtttgaa tacgcgggta tatttcgcgc ctttaccggt
      361 tgtgtgggcg gcaagccgct gttgcggatt cgggctgatg ccgcaataga acgcgctgtt
      421 ttcacacaat atcaggtaaa cgctccagtt tgacgcgttc atctgcctgc atcctgagtt
      481 ttctccccgt tccaaggcgc aactttcagc agcagcagca tacccggtag ggcgagtgcg
      541 aaacacagtt ggaaaaacgg tacataaccg agccattcaa tcagataacc ggcaaaggaa
      601 ttgatgaccg tgcgcgggac ggcggacagg ctggtaaaca gcgcaagctg cgttgcggta
      661 aatgcgggat tggtttcacg cgccatatac gatacgaacg ccgccgtccc caaccccacg
      721 ccgaccgctt ccgcgccgat aactgccgcc agcatcagcc tctcgcctgt gccgaccgtg
      781 tcgaaaggtc cgaaccctgc cagccataca aaccccaaaa cggttacagc ctgcaccgcg
      841 ccgaatagcc acaaggcttt gtttacgccg attttcagca tccacacgcc gcccaagata
      901 cctgccgcca ctgccggcca cagtcctgca tttttcgcaa tcaaaccgat gtctgtcttg
      961 ctgaaaccca tatccagata aaacggcgtt gccaacgcgg ttgccatact gtcgccgagt
     1021 ttgtaaagga agataaacag cagcacgcac accgccgaag cgatgccctt gcgcgtaaaa
     1081 aattctttaa acggctctac cacggtctgc ttcaacgttt taggaacggc aggcggcaac
     1141 acgggttcgc gcgcaagaaa cagcgtcatc agaaggccgg gcagcataaa taatgaagtg
     1201 ataacaaata cttctgacca cggcatcctg tctgccaaca ccaaactcaa tgaaccggga
     1261 atcagggcgg caatccggta ggcgttcaca tgaaccgagt tgcccaaacc caattcttcg
     1321 tctgacaaaa tctcgcgcct gaacgcatcc aatacaatat cctgactggc ggaaaaaaaa
     1381 gcgacaagca ccgacaagcc ggcaatcagc ggcagatgat tacgggggtt taaaaaggca
     1441 taggccgcca aagccgccag caaccctgcc tgcgtcagca gcatccaccc gcgccgccgt
     1501 cccaaaacgg gcagcctgac cgcgtccatc agcggcgacc acaaaaattt ccaagtaaac
     1561 ggcagaccga ttaacgccat cagcccgatg ctcttcaaat ccacctgctc gctgcgcaac
     1621 cacgccggaa tcaggttaat cagaaagtac agcggcagcc ccgaggtaaa ccccgtaaaa
     1681 atacagacaa gcatatttcg ggaaaagatt tgcccgataa aacctgattt cgatgcagtc
     1741 atcgttcgtg cagcccaaaa aaaacaaaag cctattgtag caaatatcgc ccgcctgctg
     1801 aaaaatgccg ccccgcgccg ccggtttgcc gttcaaatgc aaaatgccgt ctgaaactgt
     1861 gttcagacgg cattgctttt ctgaaaacca gacgtgctta caggctgtaa tacatttcaa
     1921 attccagcgg gtgcggcgcc atgcggatgc ggcgtacgtc ttcttctttg aaagcgatgt
     1981 agctgtcgat ccagtctttg ctgaacacgc cgccgcgcag gaggaattcg tggtcggctt
     2041 tgagggcggc cagtgcttct tccaaagaag cgcaaacggt cggcaccaat gcatcttctt
     2101 ccggcggcag atcgtacagg tttttatcgg cagggtcgcc cggatggatt ttgttttgaa
     2161 tgccgtccaa acccgccatc aacagggcgg caaatgccaa atacgggttg gcggtcggat
     2221 cggggaaacg cgcttcgatg cggcgcgcct tgctgctgtt cacggacgga atgcggatgg
     2281 aagcggaacg gtttttggcg gagtatgcca gtttggtcgg cgcttcaaag tgcggcacga
     2341 ggcgtttgta ggagttggtg gacggattgg taatcgcgtt caaggctttg gcgtgtttga
     2401 tgatgccgcc gatgtagtag agcgcggtgt cgctcaagcc ggcatagccg tcgcctgcga
     2461 acaggttttg accgtctttc caaatggatt ggtgaacgtg cataccgctg ccgttgtcgc
     2521 ccataatggg tttgggcatg aaagtggcgg ttttgccgaa gttgtgggca acgttttgaa
     2581 tcacatattt catgtcttgg gtttggtcgg cgcgtttgac caaggtggcg aaacgcgtgc
     2641 cgatttccat ttggctgccg gtaccgactt cactgtggtg gacttcgact tcgatgccga
     2701 gttcttccaa aatgtttacc atcgcggagc gcaggtcttg tcccgcgtca atcggagcaa
     2761 cgggcgcgta accgccttta acggcagggc ggtggccggt gttttgaccg tccatgtgca
     2821 ggccgcttgc ccatgcgccg ctttcggacg tgatttcgta acgggttttg tgcatgtcgg
     2881 tttcaaattc tacgccgtcg aagacgaaga attcaggctc ggggccgaag tatgccgtgt
     2941 cgccgatacc ggaagatttc aaataggcct cggcgcggcg tgcgatggag cgcgggtcgc
     3001 ggtcgtaacc ctgaccgtcg gcgggatcga taacgtcgca agtcaacaca acagtcgcat
     3061 catcataaaa aggatcgacg aaggccgtag acgcatcggg acgcaactgc atatcggaag
     3121 cctgaatgcc tttccagccg ccgatagacg aaccgtcaaa cgcctgaccg ttttcaaacc
     3181 actcttcggg gtcttccaac acgatgcgcg caggcacggt aaagtggtgc tgcttgcctt
     3241 tggtatcggt aaagcgcaaa tcgacaaagc gggcttcgct ttcttcaatc aattttacgg
     3301 cgtttttaat agacattttt cagctcctgg aaaagggaaa tagcaaaata tgcggaacgc
     3361 ggcagattag ctcaaagcca gcatgattct accacgtcca tgccgtctga aactggccct
     3421 gcgtcaaatt ccaagctgta aaatatagtt tcagacggca atattgtcaa caatatgctt
     3481 cggtttgtct gttcaaaagc ggaatacccg cccttttgtc caaatgccga caaaacagat
     3541 gccgcctgaa aacagtttag acggcatcgt tcccatcttc gcgccgccgt tataaaatga
     3601 ccgccccgcc ttgatggaat atgactatgc acgccctccc ccgctacgcc gtttttggca
     3661 accccgtcgc ccacagcaag tcgccgcaga ttcatcaaca atttgccctt caggaaggcg
     3721 ttgacattga atacgaacgc atttgcgccg acatcggcgg ttttgtacag gcggtttcga
     3781 cattttttga aacaggcggt tgcggggcaa acgttactgt accgttcaag caggaagcgt
     3841 ttcatctggc ggacgagcat tccgaacgcg ctttggcagc aggtgcggtc aatacgctga
     3901 ttctgttgaa aaacggcaag ctgcgtggcg acaataccga cggtatcggt ttggccaacg
     3961 acatcacgca ggtcaaaaac attgccattg agggcaaaac cattttgctt ttgggcgcgg
     4021 gcggcgcggt gcgcggcgta attccggttt tgaaaggaca ccgccctgcc cgtatcgtcg
     4081 ttgccaaccg cacccacgcc aaagccgaag aattggcgcg gcttttcggc attgaagccg
     4141 tcccgatggc ggatgtgaac ggcggttttg atatcatcat caacggcacg tccggcggct
     4201 tgagcggtca gcttcctgcc gtcagtcctg aaattttcct cggctgccgc cttgcctacg
     4261 atatggttta cggcgacgcg gcgcaggagt ttttgaactt tgcccaaagc aacggtgcgg
     4321 ccgaagtttc agacggactg ggtatgctgg tcggtcaagc ggcggcttcc tacgccctct
     4381 ggcgcggatt tacgcccgat atccgccctg ttatcgaata catgaaagcc atgtaatatg
     4441 ttccgcatcg tcaaatggct gattgccctg cccgtcggca tctttatctt tttcaatgcc
     4501 tatgtgtacg gcaacatcat tacctaccgc gccgtcgcgc cccatcagac tgcctttatg
     4561 tcgatgcgga tgaagcagtt tgaacaggaa ggtcgcgatg tcgcactgga ttaccgctgg
     4621 atgccctaca accgcatctc ggtcaacctg aaaaaagccc tgattgcttc cgaagatgcc
     4681 cgtttcgccg ggcacggcgg cttcgattgg ggcggcattc aaaacgccat caggcgcaac
     4741 cggaacagcg gcaaagtgaa ggcgggcggc tcgaccatca gccagcagct tgccaaaaac
     4801 ctcttcctca acgaaagccg cagctatatc cgcaaaggcg aagaagcggc gattaccgcg
     4861 atgatggaag ccgttaccga caaagacagg atttttgaac tgtatttaaa ctcaatcgaa
     4921 tggcactacg gcgttttcgg cgcggaagcc gcgtcccggt atttttatca aatacccgcc
     4981 gccaagctga ccaaacagca ggcggcaaaa ctgacggcgc gcgtccccgc cccgctctac
     5041 tacgccgacc atccgaaaag caaacggctc cgcaacaaaa ccaatatcgt gctcagacgc
     5101 atgggttcgg cagagttgcc tgaaagcgac acggactgat tgtttcagat atggaaatgc
     5161 cgtctgaact gcggttcgga cggcatatgt tttctggttt ttatagtgga ttaacaaaaa
     5221 tcaggacaag gcgacgaagc cgcagacagt acaaatagca cggaatcgat tcacttggtg
     5281 cttcagcacc ttagagaatc gttctctttg agctaaggcg agccaacgct gtaccggttt
     5341 aaatttaatc cactatacct gcccgacagc cgcaaagacc gtatcataca tagcaggcat
     5401 tgcggtgcgg gattactgcc gcaaacggca ggcaaaaaaa ataaccgctt caaggcggtt
     5461 ttttggtggc cagaggcgga atcgaaccgc cgacacacgg attttcaatc cgttgctcta
     5521 ccaactgagc tatctggcct ttgtctgtct cgttaagaga tgcgtattaa accaaattac
     5581 gggacgacat gcaagcatat cgggcaatat tctttgaaaa caggtttaag tcaatgtttt
     5641 aaatttgaat taatttatgt aaaaacaaca taaatccgta tttttgccgc ctgcccgacg
     5701 atattaggtc gcccgtctga aaaatatttt caatatttga agttcttacc cagataaaca
     5761 gaacgaacct gttcgtttcc gaccaaatca tcaggttttc ccgatgccaa caccgtgccg
     5821 tctgaaataa tgtaggcccg atcgcagatg ctgagggttt cgcgtacgtt gtggtcggta
     5881 attaatacgc cgataccgcg cgacttgaga aatccgatga ttttttggat gtcgatgacg
     5941 gcgatgggat cgacgccggc aaaaggctcg tccaacaaaa taaaatgcgg tttcatggca
     6001 agtacgcggg cgatttcgac gcgtcggcgt tcgccgcccg acaaagatgg ggcaggatta
     6061 ttgcgcaaac gctcgatatt caggtcggca agcagttttt caacttcgcc gtcgatgcgg
     6121 cttttgtctt tggtactgat ttctaaaatg gcgcggatgt tttgttcgac ggtcatttta
     6181 cggaaaatag aggcttcctg cggtaggtag ccgacaccca ggcgggcgcg ttcgtgtatg
     6241 ggcaggtgac gcaattcctg cccgtccagc atcacgctgc ccgcatcggc ggcaatgagt
     6301 ccaacaatca tgtagaagct ggtggtttta cccgcaccgt tgggcccgag caggccgacg
     6361 acttccccgc tttcgatttc aagggagaag cttttaacga cttggcgttt tttgaaactt
     6421 ttttgcaggt tttgaacaac aaggcggctg acgtttgcgc tcataaatat ctcttcaata
     6481 tcttcggtcg gcaaatgccg tccgaactag gtttatgttt cagacggcat tttgggatta
     6541 ttcggatttt tgcgtactcg aaggctggat aacgacgctg accctgccgg atttggaagc
     6601 ggattttgcg ccggattttg tgctgccgct gatggtatag acttcggttt tggtgttgta
     6661 tgtaatcacc gcaccttcgg cgacatcgcc gccgcgctgt actttggcat taccggttaa
     6721 gactacggtg ctgcctgcag atgaataagc aacgttgttt gcctgtccgc gcaccgtgcc
     6781 tttgccgccg tccaatgtct ggctgaagcg gactggcgaa ccttccgccc tcacggattc
     6841 gccgcctttg ccgccgcgtg taacattgac gcgggcggcg gaaatattga gcgtaccctg
     6901 tctgatgacg acgtttccgc tgaatgtggt gctttggttg gcttgatcga gcgaaccttg
     6961 gtcggcctca atctgaatag gctgcctgct gtcgctttga agggcaaaag cgggggacgc
     7021 cgaaaaaaat tctattaaaa caaatagctt acatatcttt tgtatcataa atcgtggctt
     7081 tcactttaga tgagaagttc aacatgcctg ttttgtggtc gtaagtcatg ccgcccgcct
     7141 gaccgtgcga tgcaccatat tggaaactga caggcgtatc ggtttgggca tattgagatt
     7201 cggtatcgac gtgcagcttt tcggcttcaa ctttacccgc ctgccgtttg ccgtcggcgg
     7261 ttttggtcag cacaacgttg tttttaaaaa gaacctgttt gttttcggta tggtaaacgg
     7321 cttcatcgct gccgacttcg tacaacaacc tgccttcttg gaagaacacg agatgcggtg
     7381 agtcgaaatg aatgtcgctg ctttcgggaa actgtttcgc gcccttcgaa ctcaaatgtt
     7441 ctttcaagta tccctgttcg tcaaaccgcc tgccgtccaa gccgtccatt gtgtattgcg
     7501 gttcgtcggg attgagcctg acttcctcga tttcgacttc gctgatgcgt cccaaccatg
     7561 ccgacaggct gcccaaggca accgccaata tcaatgggaa cgcaattccg taccgccatc
     7621 ttactttcat ttgatgtact cgttcaaagc cgcgcccaaa gtcccttgcg cctgcataat
     7681 caggtcgcac acttcgcgca ccgcgcccgc accgccgggg cgtttggcaa tataggcggc
     7741 gtgttgcagc gtaaaccaat gcgcgtcggg gacggcaacc ggcaatccgc agcgtaccat
     7801 aacgggcaaa tcgaccacgt cgtcgccgac aaaggcacac tcggcttctt ccacgccgac
     7861 ctgagcgcgc agctcttcat acgcggcgcg tttgtccgaa atgcctttga agtagtaatt
     7921 tatgcccaac tgtttgacgc ggatgccgac ggagggcgcg tcccgacctg tgataatcgc
     7981 agtctgcacg ccgcttgcct gaagcatttt cagaccgtgt ccgtccagtg tgtgaaacga
     8041 tttgatttct tcgccgttat cgcggataaa gattcgcccg tcggtcaaaa cgccgtccac
     8101 atccaatatc agcagtttga ttttggcggc gcgcgcctgt aattcgggag aaattgcctg
     8161 catgggattt ccttgttttc agacggcata tcttaccaca aatgccgtct gaaacggctg
     8221 caacgcctta tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
     8281 cagatagtac ggaaccaatt cacttggtgc ttcaacacct tagagaatcg ttctctttga
     8341 gctaaggcga ggcaacgccg tactggtttt tgttaatcca ctatacaatc cgcgccgcca
     8401 aaaggtcgtg catattcagc gcgccgatca gtacgccgtc ggcatcagta accagaagcc
     8461 cattcacatg gtttgcctgc atgactttca gggcttcggt ggcgagacgt tcggcggaga
     8521 tggttttagg atgcgtatgc atgacttcgt ctatcgaaag accggtaaaa ttgtcgcatt
     8581 cttgaaacag gcggcgcaaa tcgccgtcgg tgaatacgcc tttcagacgg cattgcccgt
     8641 ccgttaccgc caacatgccc agcccttttt cactcatgct gacgatggct tctttcaagg
     8701 gcgtgccgag tcggacggca ggcaggccgc cgcctttgtg cataatgtcg gcaacgcgca
     8761 aaagcaggcg tttgccgagg ctgccggcag ggtggctcaa ggcgaagtcg tcgggcgtga
     8821 acgcacgcgc acgcagcagg acgaccgcca acgcatcgcc caaagccatg acggcggtgg
     8881 tgctggtggt cggggcaagc cccagcgggc aggcttcttg cgaaaccgat gcggtgatat
     8941 ggatgtcggc atggcgcgcc atggttgaat cggggcgggc ggtaatgcag acaagcgtga
     9001 tgtttttgcg tttgagcgcg gggatgatgg cggcgatttc atcgctttca ccggaattgg
     9061 aaatggcgac gaccacgtcg ttgtccacaa tcatgcccaa atcgccgtgt gccgcttccg
     9121 cagggtggac gaaaaacgcg ggcgtgccgg tcgaggccat ggttgccgcc attttgcgcc
     9181 cgatatgtcc cgacttgccc atgcccgtga taacgaccct gcctttgcag tgcaacaacg
     9241 cgtccgccgc aagggcgaag ttttcgtcca aatccgccgc aatttcgcgc aagccttccg
     9301 cttcggtgtg caacacttcg cgcgcccagt cgagatattt tccgttttct gccattgcca
     9361 tccgcctttg ctgtaacaaa actacccaaa atttccgaat cggggtagca taagcattca
     9421 aaccccgcca tccatccaaa ggaatcagaa atgactattt tatcggacgt taaagcatta
     9481 ggacaacaaa tctggctgga caacctttcc cgctcgctag tgcaaagcgg cgaattggcg
     9541 caaatgttga aacaaggcgt gtgcggcgta acttccaatc ccgccatttt ccaaaaagcc
     9601 ttcgccggcg acgcgcttta tgccgacgag gttgccgccc tcaagcagca aaacctcagc
     9661 cccaaacaac gctacgaaac catggcggtt gccgatgtgc gtgccgcctg cggcatctgt
     9721 cttgccgaac acgaatccac cggcggcaaa accggtttcg tcagcctcga agtttcgccc
     9781 gaattgtcca aagacgcgca aggcacggta gaagaagcgc gccgcctcta cgccgccatc
     9841 gggtgcaaaa acgcgatgat taaagtgcct gccaccgacg caggcatcga tgcgctcgaa
     9901 accctcgttt cggacggcat cagcgtgaac ctcacgctgc tgttctcacg cgcccaaacc
     9961 ctcaaagcct acgccgccta cgcgcgcggc attgccaaac gcttggcagc tggacaaagc
    10021 gttgcccata tccacgtcgt cgccagcttc ttcatctccc gcgtggacgg cgcgctggac
    10081 acaacactgc ccgaccacct caaaggcaaa atcgccatcg cccttgccaa agccgcctac
    10141 caagactggg ctcaatactt cggcagcccg gaatttgccg cgctggaagc ccaaggcgca
    10201 aaccgcgtgc agcttttatg ggcatccacc ggcgtgaaaa acccggccta tcccgacaca
    10261 ctctacgttg acagcctgat cggcgcgcac accgtcaaca ccgttccaga tgccacgctc
    10321 aaagccttta tcgaccacgg cacagccaaa gccacgctga ccgaaggcgt agaagaagca
    10381 caagcgcagc ttgccgaaac cgccgcgctc ggcatcgatg tcgaaacctt ggcggcacgt
    10441 ttgcaggaag acggtttgaa acagtttgaa gaagcgtttg aaaaactgct cgcaccgttg
    10501 gtttaaactt ttcatccccg atgccgtctg aaaggctttc agacggcatc gcccgtttga
    10561 ccggacacag tgcggcatat caaggattca ccctaaaaat attaatgggc aagaagttaa
    10621 tgcacgacat tacccaccaa cacaaaagga aatagaatca tggcaaaagc actcgaaatc
    10681 atttcaccca atgaaattta ttcagatctg attttttaag gatccggtac ctccccatac
    10741 aatatataca gaattgatga aactggttag tagaaatgca agtaatgaga gaatcgcaga
    10801 tagagccttc gattttttct cgtccggcct tatggatggt tcagctaccg aagagcaata
    10861 taatgcccta tatgatttaa ccttgcttga agagcccggg atggaactga ataaagatga
    10921 gataatggct ttgattcata gtcttaaata aaggggctgt accggatatg tacacaggac
    10981 gtttcgcccc cagcccgacc gggctgctcc acatcggctc gctgctgacc gccgccgctt
    11041 cctatgccga tgcacgctca aacggcggca aatggctggt ccgcatggaa gacctcgatc
    11101 cgccgcgcga aatgccgggg gcggcaagcc atatcctgca cacgcttgag gcattcggat
    11161 tcgagtggga cggagaagtc gcctatcaga gccgccgtta cgccctgtat gaagaaaccc
    11221 tatgccgtct gaaaaccgcc ggactggtct atccctgcca ttgcagccgc aaagactggc
    11281 aggctgcggc agtccacggc gcagacggat tcgtctataa cggacgttgc cgcaatccgc
    11341 agcaacgccc tgcaccgcaa ggcaaacagc cggcgtggcg catccgcgtc cccgaccgca
    11401 ttatcggttt ttcagacggc atcgtcggcg gttacgccca aaacctcgcc cgcgacatcg
    11461 gcgatttcgt cctgcttcgt gcagacggtt actgggcata ccaactcgcc gtcgttgccg
    11521 acgatgccga acagggcgtt acccacatcg tccgcggaca agacctgctc gtttccactc
    11581 cgcgccaaat ctatttgcag cagtgtttgg gcgttccgac accgcaatat gcccacctgc
    11641 cgctgctgac caacgcacaa gggcagaaat ggtcgaaaca gacgctcgcc cccgcattgg
    11701 atttaaaccg ccgcgaacaa ctcctccgcc aagtgttccg ttacctcaag ctgcccgaag
    11761 caccggaaac cgaccgccct gccgaactgc tcgactgggc ggtggcacac tgggatatgg
    11821 acaaagtgcc gaaacacgcc attaccaccc cctaaacacc gcccgacaaa cgcacaaatg
    11881 ccgtctgaaa ctttcagacg gcatttcaca tacagccccg ccctattcgc gcgtatttgc
    11941 ccgttcgact tcttcccacg cttcgagaat caggccgccg tcgttgtctt tcaagagcat
    12001 cgcgtcggaa agcatacgcc gccaagtccg cgcacctttc aaaccgtgca tcagcccgag
    12061 gctgtggcgg acgatgtggc gcaagattgt gccgcgtccg gcttggatct gggcttggct
    12121 gtatgtgtag aggcactgca ccaaatcggc gtattcgatc gggctgcgag tatcgccgta
    12181 aaacaatctg tcccattcgc gcatcaccat cgggttgtgg tacgcctcgc gcccgaccat
    12241 cacgccgtca acgtgttgca ggtgtccggc gattgcttcg ttggtggtga tgccgccgtt
    12301 gatgatgatt tccagcccgg gaaactcctg cttgaggcgg taaacgtaat cgtatttcaa
    12361 cgggggaacg tcgcggtttt ctttggggga aagaccgtcc agccaagcgt tgcgggcgtg
    12421 gacgatgaag gttttgcagg cggttttgtc gcgcagcgtg ccgacgaaat cggcaacggt
    12481 ttggtattcg gtctgcctgt ccacaccgat acggtgtttg acggtaacgg gaataccgac
    12541 cgcgtcctgc atggcgttga ggcagtcggc aaccagcccg acttcgttca tcagacacgc
    12601 gccgaacgag cctttctgca cgcgcggact ggggcagccg cagttgaggt tgacctcgtt
    12661 gtaaccgtat gcctcggcgg ctttggcggc tttcgccaaa tcggacggat cgctgccgcc
    12721 cagttgcagg gcgacgggct gctcgccttc gttgaacatc aaaaagcggt ctttgtcgcc
    12781 gtaaacaatc gcaccggcat tgaccatttc gctgtacagc caagtatttc gggtaatctg
    12841 gcgggcaagg taacggtagt gcctgtccgt ccagtcgagc atcggggcga cggacaggcg
    12901 gcggggagga agggtttgcg tgttgtcgtt cattgtcgat atttcatcat tgcggattca
    12961 atttcaatcc ggaacaacct tcatttttgg ggaaatccgc gtcatgaaag agtccagcag
    13021 tatggttacc ggcccgtcgt tgcagagcga cacctgcata tgcgtgcgga aacgccctgt
    13081 ttcgacatga attccgtgtc cgcgcaacag ttccgccgtt cgcaggtaaa gctgctgcgc
    13141 ctgttctgca ggtgcggctt gcgaaaacga aggccgccgg ccgcttgccg catcggcata
    13201 aagcgtaaac tgcgacacca gcagcacgga tcccccgaca tccttcaaag acaggttcaa
    13261 cttgcccgct tcgtcttcaa acacgcgcaa atgggcgatt ttgtcggcga catagcgtgc
    13321 atctttttct atgtcgctat gcgttacacc gagcagtacg acgaatccgc cgtcgatttt
    13381 gccgcaggtt tccgtgccgt tttcggacac gacatccacc tttgcaccta ccgttttctg
    13441 tatgaccgca cgcataagcc gtcctcctta cctgaacgtg caatgccgtc tgaaaaggct
    13501 tcagacggca tcggtattgg aaatgccatt acggtttgac accgttttct tccaaaatgg
    13561 catcgatttt caagcgggct ttttcctgtc tcaaaccttg ttccaactgg tttctgacca
    13621 actcgaaagg ctgcgcgtcg gggtttttcc cgacctcgct gagtttgaac agataatagc
    13681 gttcgcccaa tttgaccgga tcgcgggtaa cgtcgccccg attcatcgct gcaaactgcg
    13741 aagccagcgg ctcgggaagc tgctgcgcca taatgaaacc gtcaaacgcc tgctcgtcgt
    13801 tcggataacg cttcatcagc ccttcaaaag acagcccttt gagcaggagc tgctgcgcct
    13861 gacgcgcctc ctcttcggtt gcgaagctga cctgctgcaa tttgatcatg cggatttgcc
    13921 gctcataaaa ctgacgcagt gcgctttcgg aaaccgtttc cgaacgttcc agaaaacgga
    13981 cgtactcctc ggcataaaaa gacgcttcgg cgattttaaa gcggttttgg acatccttat
    14041 ccttatccaa accttccttc aatgccctgt ttttcaaaac ttccaaagtt tgcagccgac
    14101 ggacggcatc gtttcggatt gcctgcccgt ccggtttttg ggactgctcc gcatgccggt
    14161 ctgcctgctg catgatctgc gccaccagcg tatccaccaa agccgggtcg atttcgggtg
    14221 ctttggctgc cgcaaaacct gccaacattg cagcaataac tgcggcagcg gtttttttct
    14281 gtttcatggt attccctgtc ctgcgcggcg gcaaaacgcc tgtctgaacg tcttgccgcc
    14341 gtatcccgtt ttcagaatta ttttgcaggt ttgatgtttg ccttgcccaa cagtgcaccg
    14401 acggcacggt caatccgttc cgcctgaagg ttgccggcaa tctgtccttt catttcatca
    14461 aaagaaggca ctttcacctc gcggctgtcg ttgacataat aaacgccgta gaaatcgccg
    14521 tttttcagcg gcgttgccgt aaattcgcct tttttcaagt ccttaattgc ctgataaagc
    14581 ggcggaacac cctgttccaa atctttcagc ggcacatatc cgaccggcgc gccggtctgt
    14641 ttggtacggt cgttgaggga atattgtttc aagacggcat cgaaaccttt tttcgccttc
    14701 aagtcggcaa ccgctttttt tgcattttct tccttgtcgg tcaggatttc gcccaactgg
    14761 acttcctgcg tacctttgta aaaaccgctg atattgtcat atgcggcttt tacttcctgc
    14821 tcggaaaccg gttgggtttt ggcgatatgc aatgcgtatg cctcgccgtt caagccatat
    14881 tttaccgcct gccaaacggt tttgaaggac ggtttcttgt cgtcgcccga ctttttcgct
    14941 tcggcacgca atttggcaag cgcattttta aactctgccg accggtcgag tttcaggcgt
    15001 ttcacttcct gtgcgaccac ggtgttgacc acttcgtttt ccagcaggga ttggcgcagt
    15061 tgcggcgtgt cttcggcacg gctgttttcc gcacggaatg cggcaacctg cgcatcgatg
    15121 acggaactgt cgattttctg accgttgacg gttgccagcg tttgggcaaa caggctgccg
    15181 gaacaggcaa gcagtgcaac ggaagtcagg atttttgctt tcataatctt ctctctttaa
    15241 attttaaatt gaaatgttcg ggcagacttc attcagtccg ccgtatggaa atactcgtca
    15301 ggggtagccg cccggatgcc gagcgcgtga atcatgccgc ctgaaaacaa atctttgagc
    15361 agcgatttga ccgttttctg gcggttcagg cggcttacgc cttcaaaacg gccgctgacg
    15421 accaaaacgg cataatgtcc gccgccggta ttgcccgcgt gtcctttgtg cagatggctc
    15481 tcatcgccga tttccaacac cagcggatcg agcgtctgca ggcgttcgcg gatcaaatcg
    15541 acggccggca ttatttgaac accgctttaa acggcttgat cagcacttcg ctgtacacgc
    15601 ctgcatgaac atagggatcg tcttccgccc acgcctctgc cgcatccaaa gactcgaact
    15661 gcgccacaat caaactgccc gaaacgcggt tggagtcctc cggcaaagga ttcgggcctg
    15721 ccgtcaacag ccggccttcc gacttcagcg tctccagccg tttgaggtgt tcgggacgtg
    15781 ccgccatacg cgcttcatac acattctccc cgtctgttgc cagcaacata aaatattcca
    15841 cagtcaatcc tcctttttca gacaggtact cagataaata ccctgaataa taaagaaaac
    15901 aagcatcagt gcagtcgagc cgaacatttt atagttgacc cattgcgact cgaaccgggt
    15961 aaacacaaac cagttggcga tacccataaa aatcaggaaa ccgacccaca tatacgtcaa
    16021 tttcgcccat acggcatccg gaagctgaat ctccctgccg atactcgcct tcaagccgtt
    16081 tttaccggcg aggtggctgc cccacaggaa cagcgcgccc agccaaaaca aaacgctcgg
    16141 cttccacata atgaagcggc tgtcgcccaa aacaatggtt gcgccaccga atatcacaat
    16201 cagcaccagt ccgacccact gcatcgtatc cagcttttta tatttccaat acagaaaagc
    16261 cgcctgaacc acaccagcaa ccaaggcgac cgccgtagcg gcaatcatat ttttggtaac
    16321 ggtataggtg gcgaaaaaca ggatgacgga caaaaggtcg ctgacgaatt tcatatccaa
    16381 atgagaaagc aaatcaaata aacgctaaat catacaggca gaaccctgtt tttgcacctg
    16441 caaaaccaac aaattttgtt ttgattgatg caaatatgca cagatgtttt tgaaaaaaga
    16501 tggagatatg tcataatttg taaaaacggc gaatgattgc cgtttaaaat gtggcggcgg
    16561 tcggtacatt cacatattaa atgcccgccg gccgactgcc ccgccgatgc ccgatgatga
    16621 cgaaatttac agactgtacg cggtcaaacc gtattcagcc gccaacccac aggggataca
    16681 tcttgaaaaa caacagacaa atcaaactga ttgccgcctc cgtcgcagtt gccgcatcct
    16741 ttcaggcaca tgctggactg ggcggactga atatccagtc caaccttgac gaaccctttt
    16801 ccggcagcat taccgtaacc ggcgaagaag ccaaagccct gctaggcggc ggcagcgtta
    16861 ccgtttccga aaaaggcctg accgccaaag tccacaagtt gggcgacaaa gccgtcattg
    16921 ccgtttcttc cgaacaggca gtccgcgatc ccgtcctggt gttccgcatc ggcgcaggcg
    16981 cacaggtacg cgaatacacc gccatcctcg atcctgtcgg ctactcgccc aaaaccaaat
    17041 ctgcactttc agacggcaag acacaccgca aaaccgctcc gacagcagag tcccaagaaa
    17101 atcaaaacgc caaagccctc cgcaaaaccg ataaaaaaga cagcgcgaac gcagccgtca
    17161 aaccggcata caacggcaaa acccataccg tccgcaaagg cgaaacggtc aaacagattg
    17221 ccgccgccat ccgcccgaaa cacctgacgc tcgaacaggt tgccgatgcg ctgctgaagg
    17281 caaacccaaa tgtttccgca cacggcagac tgcgtgcggg cagcgtgctt cacattccga
    17341 atctgaacag gatcaaagcg gaacaaccca aaccgcaaac ggcgaaaccc aaagccgaaa
    17401 ccgcatccat gccgtccgaa ccgtccaaac aggcaacggt agagaaaccg gttgaaaaac
    17461 ctgaagcaaa agttgccgcg cccgaagcaa aagcggaaaa accggccgtt cgacccgaac
    17521 ctgtacccgc tgcaaatact gccgcatcgg aaaccgctgc cgaatccgcc ccccaagaag
    17581 ccgccgcttc tgccatcgac acgccgaccg acgaaaccgg taacgccgtt tccgaacctg
    17641 tcgaacaggt ttctgccgaa gaagaaaccg aaagcggact gtttgacggt ctgttcggcg
    17701 gttcgtacac cttgctgctt gccggcggag gcgcggcatt gatcgccctg ctgctgcttt
    17761 tgcgccttgc ccaatccaaa cgcgcgcgcc gtaccgaaga atccgtccct gaggaagagc
    17821 ctgaccttga cgacgcggca gacgacggca tagaaatcac ctttgccgaa gtcgaaactc
    17881 cggcaacgcc cgaacccgct ccgaaaaacg atgtaaacga cacacttgcc ttagatgggg
    17941 aatctgaaga agagttatcg gcaaaacaaa cgttcgatgt cgaaaccgat acgccttcca
    18001 accgcatcga cttggatttc gacagcctgg cagccgcgca aaacggcatt ttatccggcg
    18061 cacttacgca ggatgaagaa acccaaaaac gcgcggatgc cgattggaac gccatcgaat
    18121 ccacagacag cgtgtacgag cccgagacct tcaacccgta caaccctgtc gaaatcgtca
    18181 tcgacacgcc cgaaccggaa tctgtcgccc aaactgccga aaacaaaccg gaaaccgtcg
    18241 ataccgattt ctccgacaac ctgccctcaa acaaccatat cggcacagaa gaaacagctt
    18301 ccgcaaaacc tgcctcaccc tccggactgg caggcttcct gaaggcttcc tcgcccgaaa
    18361 ccatcttgga aaaaacagtt gccgaagtcc aaacaccgga agagttgcac gatttcctga
    18421 aagtgtacga aaccgatgcc gtcgcggaaa ctgcgcctga aacgcccgat ttcaacgccg
    18481 ccgcagacga tttgtccgca ttgcttcaac ctgccgaagc accgtccgtt gaggaaaatg
    18541 taacggaaac cgttgccgaa acacccgatt tcaacgccac cgcagacgat ttgtccgcat
    18601 tacttcaacc ttctgaagta cctgccgttg aggaaaatgc agcggaaatc gttgccgatg
    18661 atttgtccgc actgttgcaa cctgctgaag caccggctgt tgaggaaaat gtaacggaaa
    18721 ctgttgccga aacgtccgac ttccacaccg ccgcagacga tttgtccgca ctgttgcaac
    18781 ctgctgaagc accggccgtt gaggaaaatg taacgaaaac cgttgccgaa atacctgatt
    18841 tcaacgccac cgcagacgat ttgtccgcat tacttcaacc ttctgaagta ccggccgttg
    18901 aggaaaatgc agcggaaatc actttggaaa cgcctgattc caacacctct gaggcagacg
    18961 ctttgcccga cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct
    19021 cggaagaaaa tatcccaaat aatgcagata ccggtttccc ttcggaatct gtaggttctg
    19081 acgcgccttc cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg
    19141 atgccgctgc cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac
    19201 gtgcccaagc attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca
    19261 aggatgccgt ttcagacggc atcttttttt gccttatcgg tgtaacggat aaagtttgaa
    19321 ccggcacagg ctcaaacagc aggccgacgg caacaaaatg ccgtctgaaa cccctaaagg
    19381 cttcagacgg cattggcggc ggcggtatca ggcagtttga taggcaaccg aatcgtcacc
    19441 gcttttcttt tgaatgaact cgattttgta tccgtcgggg tcttcgacga aggctatcac
    19501 ggttgtgccg tgtttcatcg ggccggcttc gcggacgacg tttccgccct gccgcttcac
    19561 acgttcgcag gcttcgtagg catcgtccac ttcaaccgcg atgtgtccgt aggcgttgcc
    19621 caagtcgtat cgttccgtat cccagttgtg cgtcagttcc aaaaccgtgc tgtcggtttc
    19681 gtcgccgtaa ccgacgaagg caagggtaaa tctgccttcg ggataatctt ttcggcggag
    19741 cagtttcata cccaaaacgt tttggtagaa atcgagggat ttttcgagat tgcccacgcg
    19801 gagcatagta tggagtaagc gcatttttgt gttcctctcg gtagtggtta aacttcgatt
    19861 ttgtttggcg taaacgtctg ccatttgttg caggcaggac aatgccagaa aaagacttgt
    19921 gatttgaagt ggcagtttcg gcaccggtac atcacgctgc gctgtagctg ccgtccgata
    19981 accgaacgca tcatatcggc atcggctttc caagccggat ccaaatcgct gagtttcaaa
    20041 ccaagcaggc ggtacacgcc gttgaggtcg ggcttgcggc ggacaagctc gacggcggtt
    20101 tgcgcggctt ctttctcgca cttaagcagc agggatttct cgtacacgac attgatcagg
    20161 tcaagttcgg gaaacgtctg catatatcct gtcagacggt tcaagccttc ttcaggtttt
    20221 ccctgcgcgg cataggcttc gtaaagcttc tcgccgacca tgctcaagta tgcatggttt
    20281 tgctgctcga tggcggcata ggcttcgacg gcggcaggga aattgccttg tcggtgttcg
    20341 atgtcgccca aaatcatgtt ggcacgggtg cattttttgt tggcttcgag tgccttgccg
    20401 acattgaaac gcgcgacatc gaaattggac ttgaacagcg cggcttgggc aagttcgcaa
    20461 taaaactggg cgatttcaaa ctgataggtc tgatcgtcat ggctgagcag ccgggcggtt
    20521 tcaaccgctt tttcccaatc cctgtcctgt tggtagatat tgagcaggtg ctgtctggct
    20581 tcacgcgcca ttttaccgtc ttgcagcccc aaaaaaatct gttcggcacg atcgaccaac
    20641 cccgcacttt ggtagttttg cgccaattca aacaggacgc gcgcgcgctt ttcgccgacc
    20701 gtatcgggag aatcgagcat tgtccggtgt atgttgatgg ctttgtcgtt ttcgccacgc
    20761 tgacggtaaa gcttgcctag ggtgaggttt aggtcgtatg actgaggctg ctgatcaatg
    20821 acctctgcca attccctggc ggcacgtccg ctgttgcggt caaccaaggc atccagactt
    20881 ttataaaatc ccgacggtat gctttttgcc tgctttaata cagtcttcat atccacacgg
    20941 gcggcaaacc agcccatcgc gaagaaaacg ggcaaaagga taatcggcag caggataatc
    21001 cacaattcat tgtccatatc ggctttctca aggctgtttg gcagattcgg gcgcattttg
    21061 cgccggtggt gcggtcagct ctttccctgt caaacgcgca tttttcttta cttcggcacg
    21121 caacctgccg ttctcgccac gtaacgacaa caaccgtccg aacaaggcaa acattccaaa
    21181 aacaataccg actacaaatg cgccgaacaa tacgacaatc agcggcaaat cgaatttttg
    21241 ccccggcagg taggaaaagg taacggcatc cgtattaatg acggcaagca gcaggaagag
    21301 cagcaggata atgattttga tgacggtata gataagtttc atgtttgctc cgtaaataaa
    21361 aacgccgccc ggacagcttc gcactctgaa ccaagattgc gctagtttaa acgatttgca
    21421 cggtttcgga taggatgcgg cagcgtgttc ggacgacata cggagtatgc gattgccgac
    21481 aattttacca aatacaccgt tcctttccat ttgaaaaata acggattgga caccgcatcg
    21541 acagaaaaac ccgccgcgca cttgtcaaaa ccctgtttgc aggcgtatct ttacaatctt
    21601 caaattcaaa ccgttcattg aaacatatca gaataagaaa ggctttacat catgagcaga
    21661 cccgtacccg ccgtattcgg cagcgttttt cacagtcaaa tgcccgtcct cgcctaccgc
    21721 gaaggcaaat ggcagccgac cgaatggcaa tcttcccaag acctctccct cgtaccgggt
    21781 gcgcacgccc tgcactacgg cagcgaatgt ttcgagggac tgaaagcctt ccgtcaggca
    21841 gacggtaaaa tcgtactgtt ccgtccgact gccaatatcg cgcgtatgcg gcaaagtgcg
    21901 gacattttgc acctgccccg ccccgaaacc caagcctatc tcgatgcttt ggtcgaactg
    21961 gtcaaacgtg ccgccgacga aattcccgat gcgcctgccg ccctgtacct gcgcccgacc
    22021 ttaatcggta ccgatcccgt catcggcaag gccggttctc cttccgaaac cgccctgctg
    22081 tatattttgg cttcccccgt cggcgactat ttcaaagtcg gttcccccgt aaagattctg
    22141 gtggaaaccg aacacatccg ctgcgccccg cacatgggcc gcgtcaaatg cggcggcaac
    22201 tacgcttccg ctatgcactg ggtgctgaag gctaaagccg aatacggcgc aaatcaagtc
    22261 ctgttctgcc cgaacggcga cgtgcaggaa accggcgcgt ccaactttat cctgattaac
    22321 ggcgatgaaa tcattaccaa accgctgacc gacgaatttt tgcacggcgt aacccgcgat
    22381 tccgtactga cggttgccaa agatttgggc tataccgtca gcgaacgcaa tttcacggtt
    22441 gacgaactca aagctgcggt ggaaaacggt gcggaggcga ttctgaccgg tacggcagcc
    22501 gtcatctcgc ccgttacttc cttcgtcatc ggcggcaaag aaatcgaagt gaaaagccaa
    22561 gaacgcggct atgccatccg taaggcgatt accgacatcc agtatggttt ggcggaagac
    22621 aaatacggct ggctggttga agtgtgctga tgctttaaat aaaaaatgcc gtctgaaacc
    22681 cgtttggcgt ttcagacggc attttcgcat ccgaaccgtt tccgccgcac ctgcagcaag
    22741 tcggcacaaa ggcaatcggt taaaacaagc gtccgcattt cccatcccgc ctgccgtaag
    22801 tcgggcattt ctctagaaat acgcttcaga cggcaaaccg ccgcccgaaa ccgatatgtg
    22861 gcacggacgg cgcacggatt tgaaaacggc ggattatccc tcggtgctca aggcattgat
    22921 gctgtaaccg ccgtcaacgt aagtgatttc gccggtaatg ccggacgaca ggtcggacag
    22981 caggaaggcg gcggtattgc cgacttcttc gatggtaacg ttgcggcgca gcggattgtg
    23041 ggcggcgacg tgtcccaaga gtttgccgaa atcggcgatg ccggaggcgg cgagtgtttt
    23101 gatcggaccg gcggaaatgc cgttgcagcg gatgccttct ttgcccagac aggcggcggt
    23161 aaaacggatg cccgcctcaa ggctggcttt tgccataccc atcacgttgt aattcggaat
    23221 cgcgcgcacc gcgcccaagt agctcagggc aacgatggcg gagtttctgc cgcgcatcat
    23281 cggacgggcg gcttttgcca atgcgggcag gctgtatgcg gaaatttcgt gtgcggtgtt
    23341 gaacgcttcg cggctgatgc tatcgaggaa gtcgccgctc aaggcttctt tcggtgcgaa
    23401 gccgatggaa tgcaccaaac cgtccaagcc gtcccaatgt ttgcccaaat cgacgaacac
    23461 ttggttgatt tcgtcgtcgc tggcgacatc gcagcggaac acgagttcgg aatccaattc
    23521 cgccgccatt ttgcggacgc gctcttccag tttgtccaca acgtaggtaa acgccagttc
    23581 cgcgccttgt tcgcggcagg ctttggcgat gccgtaagcg atggaacgct cggaaatcat
    23641 gccggtaatc agaatttttt tgccttgcag aaaacccatt ttgtatcctt caaatagtgt
    23701 tcggttcgtt ctaaaccgtt gattatagca aattgtccct gtttctgtgt tttcacgttg
    23761 cagcatgcaa acggcaatgc cgtccgaagc ggatttcaga cggcatcgga cgtttcaaat
    23821 acggtttaag gcatcagatg ccgcgcaaca attcgttcac gctggttttc gcacgggttt
    23881 gcgcgtccac gcgtttgacg atgacggcgc agtaaaggct gtggctgccg tctttggaag
    23941 gcatactgcc ggacacgacg accgaacctg tcggtacgcg gccttggtag atttcgccgg
    24001 tggtgcggtc gaagattttg gtggattgac cgatgaacac gcccatcgaa atcacgctgc
    24061 cttcttcgac aatcacgccc tcaacgattt cagaacgcgc accgatgaag cagttgtctt
    24121 caatgatggt gggggcggcc tgcaggggtt cgagtacgcc gccgataccg acgccgccac
    24181 tcaagtgcac gtttttaccg atttgcgcgc aagagccgac agtcgcccaa gtatcaacca
    24241 tcgcgccttc gtcgacgtat gcgccgatgt tgacgtaaga cggcatcagc acgacatttt
    24301 tcgccacaaa gctgccgcgt cgggcaaccg cacccggaac ggcgcggaag cctgcgtttt
    24361 tgaactcgtc ttcagaccag tcggcaaact tggtcggcac tttgtcgaag tatttgttca
    24421 cgccgtcgtt gaggacttcg ttgtcttgga tgcggaagga cagcaacacg gcttttttcg
    24481 cccattcgtt gactttccac tcacccacgc ccaagcgttc ggcaacgcgc agtttgcctg
    24541 aatcgagttg gcggatggtt tccaacacgg cttctttgac ttcgggagta acggtggtcg
    24601 gggtaatgtc cgcgcggttt tcaaaggcgg tttcgataat gttttgcaaa gacataatgt
    24661 ttccttatgt gagatgtttc gtgggacgct tgccggtatt ttcaagcagt atgttcattt
    24721 gaaatgaatg cgctattgtg ccacgcaaac gcatattcgt cagcaataca cgggcggttg
    24781 ccgaacattg gcgtttcgga atcgggcggg cggcaggtat tgccttttcc gtgcccgaat
    24841 caatacagaa cggcggcggc acttttatgc cgcgccgccg cctttcagac ggcattcgcg
    24901 gtaaaacgcc atcagccctt cggtcgaggc atcgtgtacg gacgtgccgc cttccagttc
    24961 gccgatgatg gttttcgcca actgtttgcc gtactcaacc ccccactgat cgaaggggtt
    25021 gacgttccat atcgcgcctt ggacgaaggt tttgtgttcg taagccgcca tcagcatacc
    25081 cagattgtag ggcgtgaggc ggtcgagcag gatgctgttg ctggggcggt tgccggggaa
    25141 ttctttgtgc ggcgcgaggc gttcgcgttc cgcttcgggc aaatctgcca gttcggcgcg
    25201 tgcttcgtcc aaggttttgc ccttcatcag ggcttccgct tgggcaaagg cgttggcaac
    25261 ggtaaaacgg ctgcgtccgt cctccacgcc ctgcgccgtc atcgggacga taaagtcgca
    25321 gggaatcagg cgcgtgcctt ggtggagcag ttggaaatag gcgtgctggc agttgacccc
    25381 ttcaccgccg aacacgatgc cgcccgtttt gcacacggcg ggactgccgt ctgaagcgcg
    25441 gcttttgccc aaactctcca tatcgagctg gttcagccac accggcagca ggcgcaggtt
    25501 gtggctgtac ggaacggcgg tctgcccgtc cgcgtgttgg aaattgttat accacacggc
    25561 aatcagtgcc atcagaacgg gaatgttgcg gcgcggcggc gtgtggaaaa aatggctgtc
    25621 catcgcgtgc gcccccgcca acaactcgcg gaaacgcgcc ccgccgaccg caaccatcac
    25681 gggcaaaccg acggacgacc agacggaata gcgtccgccc acccagtcgt acatcgcaaa
    25741 cacgcgttcc gccgcgatac cgaaagccgc agctgccgca gtgtcggcag acaccgcgca
    25801 aaaatggcac gccgtttcgg attccgagaa ccccgcaccg cgataccacg ccttgactgc
    25861 ctgtgcattg agcagggttt ccggtgtttt gaaggacttg ctggcaacgc aaaacactgt
    25921 cgtttcgggg ttcagacggc ataaaaccgc atccaggcag gcaggatcgg cgttggcggc
    25981 aaaatggacg gcgatatgcc gtctgaacgg ctcgagtgcc tgcacgcaca ttgccggccc
    26041 gaggtcggat ccgcctatgc cgatgtggac aaaatccgta atccgtttcc cagttatccc
    26101 ctgatacgaa ccgtcgtcca aactgtgtgc aaactccaac gcacgattta actcgcggcg
    26161 gatttcgggc aacacgtccc tgccgtccac ataaacggca tccgcaccgt cgggcaggcg
    26221 caaagccgta tgcagcgcgg cacgcccctc gctgccgttg actttcgcac ccgtccgcaa
    26281 agcacgcatt ttcccttcca aatccgccgc gtcggcaaga ttgcagagca gttgcagcgt
    26341 atcttcgccc aaacggttct tgctgtaatc gaacaacatc ccgtccaaac gctcgtgcat
    26401 acgctcaaaa cggtcgggtt cgcaggcaaa gcggtcgcgc aaaaggacat gacgcgtatc
    26461 ctgataatgg cgttcgagcg catgccatgc acgggtaaaa gcattcatct gttttccttg
    26521 atttttcaga accggattaa aatgtagcag aatgtagttt aacaaacggc agcggctttg
    26581 gcgaatctcc ggaacaccgc ccgccgcgtc caatagcacc ttatttccat atggcgaaaa
    26641 tatagtgaat taacaaaaat caggacaagg cggcgagccg cagacagtac aaatagtatg
    26701 gaaccgattc acttggtgct tcagcacctt agagaatcgt tctctcttga gctaaggcga
    26761 gccaacgccg taccggtttt tgttaattca ctataccctc cggccggcat ttgacccgca
    26821 acaatatcct gcaagattta ttgtgtacgc ataaatgccg gacagccgcc taaataaggt
    26881 ataatcgcat ccgattctgt cccgtttgcc gcccgcaggc gggatgtcgg cggttttcat
    26941 cttaccggga acacgccggt actgtttttt cacgatgttt cttacatttt attccaatta
    27001 ctttacgggg ctagaatatg gctaaaaacg gaggattttc tttgttcgca aagaaagaaa
    27061 aacgctttat ctttgaaggc aggcattccg cctccgacaa actggtcaac ggcgaagtat
    27121 ccgcgtttac cgaagaagag gcgcgcaaaa aactggcaaa acgcggcatc cgcccgttgc
    27181 agattacccg tgtgaaaaca agctccaagc gcaaaatcac acaagaagac atcaccgttt
    27241 tcacccgcca gctttccacg atgattaaag cgggcctgcc gctgatgcag gcatttgaaa
    27301 tcgtggcgcg cggacacggc aacccgtcta tgacggaaat gctgatggaa atccgaggcg
    27361 aagtggaaca gggcagctcg ttgagccgcg cattctcaaa ccacccgaaa tatttcgacc
    27421 gcttctactg caacctggtt gcggcgggcg aaacgggcgg cgtattggaa agcctgctgg
    27481 acaaattggc aatttacaaa gaaaaaaccc aggccatccg caaaaaggta aaaaccgcgc
    27541 tgacctatcc ggtatccgtc atcgccgtcg ccatcggttt ggtattcgtg atgatgattt
    27601 tcgtactgcc cgcctttaaa gaagtttacg ccaatatggg cgcggagctt cccccactga
    27661 cccaaacagt gatggatatg tccgactttt tcgtctcata cggctggatg gtgctgattg
    27721 cactgggctt tgccatatac ggcttcctta aattgaaggc gcgttcgatt aaaatccaac
    27781 ggcgtatgga tgccatactg ctgcgtatgc cgattttcgg agacattgtc cgcaaaggga
    27841 cgattgcccg ctggggcagg acgacggcga cgctgattgc ggcaggcgtg cctttggtcg
    27901 atgtattgga ctccactgcc ggcgcggcgg gcaatttaat ctatgaagaa gccacccggg
    27961 aaatccgtac gcgcgtcatc caaggtttgt ctatgacttc ggggatgcgt gcgacggaac
    28021 tgttccccaa tatgatgttg cagatgtctt ccatcggcga ggaatcgggt tctttggacg
    28081 atatgctcaa caaagccgcc gaattttacg aagacgaggt ggacaatgcg gtcggcaggc
    28141 tgtccgctat gatggagccg atcattatcg tgattttagg cttggtcatc ggcacgcttc
    28201 tggtcgccat gtatctgccg ctgtttaatt tgggcaacgt ggtcgcctga tttgccgcac
    28261 agatccggcg cggattcgct ctgcgccggt ttgtttttgt tttgaataca ttgagaacaa
    28321 aatatgtctg atttgtctgt attgtcgccg tttgccgtgc ctttggcagc ggtgttcggg
    28381 ctgctggtcg gcagtttctt aaatgtcgtc atttaccgcg tgccggtcat gatggaacgc
    28441 ggctggacgg tatttgccaa agaatattta aacctgccgc tgaccgaaga ggaaagccgt
    28501 accttcaacc tgatgaaacc ggattcctgc tgtcccaaat gccgcgtgcc gatacgcgcg
    28561 cggcagaaca tcccgattgt cagctacctg ctcctgcgcg gcaaatgcgc ctcctgccaa
    28621 accaaaatca gcatacgcta tcccttaatc gaactgctga ccggcgtatt gttcgggctg
    28681 gttgcctggc aatacggctg gtcttggatt acattgggcg gtttgatact gaccgcgttt
    28741 ctgatttccc tgacctttat cgatgcggac acccaatatc tgcccgactc gatgacatta
    28801 tccttgattt ggctggggct gatatttaat ttggacggcg gcttcgtgcc tttgcagtct
    28861 gccgttttag gtgcggttgc cggctatggt tcattatggc tcttatgtgc agtgtataaa
    28921 ctgctcacaa gaaaaaccgg tatgggcaac ggagatttca aactgattgc cgcattgggc
    28981 gcgtggctcg gcatatccgc attgcccgtg ctgatttttg tttcctcgct gatcggtttg
    29041 gtcgcggcaa tcgttatgcg cgtcgccaag gggcagcatt ttgccttcgg ccccgcactg
    29101 acagtttcgg gctggataat ttttacggca aacgattccg tatggcgggc ggtcaactgg
    29161 tggctgaccc atccggtgct gtaagatgac ggtatgggtc ggactgaccg gcggaatcgg
    29221 cagcggcaaa tcggcagccg cgcaatgttt tgccgatttg ggcgtaccgc gtatcgatgc
    29281 ggatgcggcg gcgcactcgc tgacggcttc agacggcatc gccctgccgg aaatcaggcg
    29341 gctgttcggc gacaccgttt tcgacacaca gggtttgttg cgacgcgaca tattgcgtaa
    29401 agaagtcttt gcctccccat cgcgaaaagc cttgctcgaa tccgtgatgt tgccgctgat
    29461 tttctcagaa atcaaaaaac agcaagaaac atttaacgac gcaccctacg gcattatcga
    29521 aatcccactg ctgacagaaa aacgtcaatt catgaaattg atacagcgcg tgctgaccat
    29581 aagtgcccct gtggaaaaac gtatcggcag ggtgatggcc cgcagcgggc tgacgcgcgg
    29641 cgaggtggct gccgtcatca gccatcaggc atccgaatcc gaacgcctgc tgcttgcaga
    29701 cgatgtgctg ctcaatgacg gcagcctcaa aagcctgcgt gataaaacaa tgcgcctgca
    29761 cgcgttttat tcagggattt tcgcctcaaa accaacacaa ggaaaacaca atggctgaat
    29821 cgcggcaaac acgccttcaa gtcaaatgtc cgacctgtca aacagcagta gtatggaaac
    29881 cggaaaacgc attccgcccc ttctgttcgc aacgctgcaa actgatcgac ttgggcggat
    29941 gggcagacgg gaaatatacg gtttccagcc aaacggaaag tttgccggaa atatccgaac
    30001 ccgacatggc ataccgctga ccgccccgcc ttcccggcaa acaccctgaa agtcaaatgc
    30061 cgtctgaaac aaacacgctt cagacggcat tttcattctc aaacctaatc gttggtattt
    30121 gccgttacct cttccaatga agtaatgccc tgcataactt tcaaaatacc ggcccggcgc
    30181 aaatccacca taccctcctt ataggcaacg tccaaaatat ccacttccgt accgttgttc
    30241 ataatcacac gctgcatttc ttcgctgatg ggcataacct catacacgcc cgcacgcccc
    30301 ttataaccct gcccccggca acggtcgcaa ccgacggcgc ggtaaagttt ccaatctttt
    30361 gcaaggtcct catcggtgaa gccgacttcc ttcaaagcag aggcagacgg gcgttccact
    30421 tcctgtttgc agctcgaaca cagcctgcgt aaaagacgct gcgccataat caggctgacc
    30481 gaactggcaa tattaaacgg cgcgacaccc atattcagca tacgcgacaa cgtcgccggc
    30541 gcattattgg tgtgcagggt ggaaaacacc atatgccctg tttgtgccgc cttaatcgca
    30601 atatcggcag tttccaaatc acgaatctca ccgaccataa tgatgtccgg gtcctgacgc
    30661 aggaaagact tcaaagcagc ggcaaaagtc agaccctgct tatcattgac gttaacctga
    30721 ttgatgcccg gcaggttaat ctcggcaggg tcttccgccg ttgcaatatt taccgactcc
    30781 gtattcaaaa tattcaaaca ggtatagagc gacaccgtct tacccgaacc cgtcggaccg
    30841 gttaccagca ccatcccgta gggacggtga atcgcttcca acaacaattt tttctgaaac
    30901 ggctcaaaac cgagctggtc gatgttcaaa gacgcggcat cggaattcaa aatccgcatc
    30961 acgacctttt cgccaaacag cgtcggcaat gtgctgacac ggaaatcgac aggcttgccg
    31021 cccttttgaa aggtcagctg catcctgccg tcctgcggta tccgtttttc ggaaatgtcc
    31081 aaacgcgaca ttaccttaat ccgggaagca agctgccccc ttaccgcaat gggcggctga
    31141 accacctcgc ggagctgccc gtccacacgg aaacggatac gggcattgtg ttcgtaaaac
    31201 tcgaaatgga tgtccgatgc cccgctgcgc aaggcatccg acaaagtctt atggataaac
    31261 ctcggaacag ggccgtcttc tgcctcctcg ttgtcgatat acagggtgtg gctttcctct
    31321 tcctcctgcc cctccccaag ctcctgaagc agcgatgtcg aacgcgaacc cacccaatcg
    31381 agcaaacccg ccaactggtc atcctcgaca atgaccaact caacctcaat ccctgcggca
    31441 gaaacggttt tctgaatttg cggcatctgt gtcggatcgg aaaccgcaaa aaatactttg
    31501 tcgccccgac ggaaaaccgg cacacagtgg aactccacca tctgctcctc cgtcaacacc
    31561 cccatcagca ccctgtggcg cggataatga cgcaaatcaa gaatcgaata actgaacacc
    31621 ctcgcaatca atgccgcaag cgacttgggc gaaatgacac cgtctgaaaa cagcatcggc
    31681 aacacttcct tacccgcctg cgactcattg tagtaatgct cggcctgctc aacagtaacc
    31741 acctggtttt gaaccagaat cctcagcaaa cctacgctca tacgacctta tccccaaatt
    31801 tattcattgt tatacctgta cagctt
//
LOCUS       DAXHTY010000013        31301 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXHTY010000013 DAXHTY010000000
VERSION     DAXHTY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.013"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(17..1543)
                     /locus_tag="ACFNMK_000513"
     CDS             complement(17..1543)
                     /locus_tag="ACFNMK_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC6363840.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
     gene            complement(1655..3061)
                     /locus_tag="ACFNMK_000514"
     CDS             complement(1655..3061)
                     /locus_tag="ACFNMK_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6363841.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLNMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGNDTALDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLISTLMKRFGYRKVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            3345..4124
                     /locus_tag="ACFNMK_000515"
     CDS             3345..4124
                     /locus_tag="ACFNMK_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC6363842.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            4121..4822
                     /locus_tag="ACFNMK_000516"
     CDS             4121..4822
                     /locus_tag="ACFNMK_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC6363843.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            4819..6273
                     /locus_tag="ACFNMK_000517"
     CDS             4819..6273
                     /locus_tag="ACFNMK_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC6363844.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(6456..6941)
                     /gene="purE"
                     /locus_tag="ACFNMK_000518"
     CDS             complement(6456..6941)
                     /gene="purE"
                     /locus_tag="ACFNMK_000518"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC6363845.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEACVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVSVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(6989..7705)
                     /locus_tag="ACFNMK_000519"
     CDS             complement(6989..7705)
                     /locus_tag="ACFNMK_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC6363846.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGRIVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            complement(7705..8373)
                     /locus_tag="ACFNMK_000520"
     CDS             complement(7705..8373)
                     /locus_tag="ACFNMK_000520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6363847.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPKHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(8384..8569)
                     /locus_tag="ACFNMK_000521"
     CDS             complement(8384..8569)
                     /locus_tag="ACFNMK_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC6363848.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            8712..10688
                     /gene="mutL"
                     /locus_tag="ACFNMK_000522"
     CDS             8712..10688
                     /gene="mutL"
                     /locus_tag="ACFNMK_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC6363849.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIKLALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQDGSAHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVGNAGEVLHDITGVTPAPMPSENNSENLFDRASNYPTGNKPDTRNAFGASGKTAPMP
                     YQAARAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELNRLEQARFGNMPSETPTPKT
                     DAPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLNELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            10783..12912
                     /gene="dnaX"
                     /locus_tag="ACFNMK_000523"
     CDS             10783..12912
                     /gene="dnaX"
                     /locus_tag="ACFNMK_000523"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC6363850.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTSQQVADHLAHVLDSEKIAYEPVALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALLSKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTIGGEQIQLYYQIAVHG
                     KRDLSLATDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETSAK
                     KPQPRPEAETAQTSVQTASAAAMPSESKTAEPVTNQENNDVPPWEDAPDEAQTAACTA
                     QASAKSIQTASEAETPPENQVSKNKAADNVTDAPLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDGAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWHDEAGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    11972..12011
                     /gene="dnaX"
                     /locus_tag="ACFNMK_000523"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            12992..13327
                     /locus_tag="ACFNMK_000524"
     CDS             12992..13327
                     /locus_tag="ACFNMK_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC6363851.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(14158..15204)
                     /gene="recA"
                     /locus_tag="ACFNMK_000525"
     CDS             complement(14158..15204)
                     /gene="recA"
                     /locus_tag="ACFNMK_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC6363852.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGVEMHIT
                     EGKLDETDGERPEE"
     gene            15458..16222
                     /gene="aroD"
                     /locus_tag="ACFNMK_000526"
     CDS             15458..16222
                     /gene="aroD"
                     /locus_tag="ACFNMK_000526"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC6363853.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAVLSAVLDQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGAFPCSDGYYFELL
                     DALIESRLPDIIDIELFAGETAVRRAVADAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPVVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            16242..18257
                     /gene="rep"
                     /locus_tag="ACFNMK_000527"
     CDS             16242..18257
                     /gene="rep"
                     /locus_tag="ACFNMK_000527"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC6363854.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYQLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQTFMDMFVSYLAKAETSEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(18324..19382)
                     /gene="dinB"
                     /locus_tag="ACFNMK_000528"
     CDS             complement(18324..19382)
                     /gene="dinB"
                     /locus_tag="ACFNMK_000528"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC6363855.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQGLWA"
     gene            19616..20395
                     /locus_tag="ACFNMK_000529"
     CDS             19616..20395
                     /locus_tag="ACFNMK_000529"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6363856.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(20595..21980)
                     /locus_tag="ACFNMK_000530"
     CDS             complement(20595..21980)
                     /locus_tag="ACFNMK_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFC6363857.1"
                     /translation="MIVASRWPLLEKAFLRFGMPVAVVDLESTGGNLYEDRVTEVALV
                     KFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELFSVLKGCVLV
                     AHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSIIERLGIVVED
                     RHRAMADVSALCDYLEYSLSEHGVEEWIRQCFRLMNPKPLPAALPERLREQLYGLPDG
                     MGVLACFDGGGAINYIGTFERVYSEVSALLDSGKASFDWCNTEAVRFFPALGRLHAHK
                     VKAELVRHYGADCYVSAKNLLKTFTTVRFEKGSDGMLDAKTAALKNGVADNPPTGLFV
                     NKKAARRALSLWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACGRSDEQVLAF
                     AHKLPVLDWGKMHEVEITETDPLTREKVVLHGMGGALEMDGGLWYFDKDLPDVFKAKF
                     KTDRKNIKEIG"
     gene            22098..22174
                     /locus_tag="ACFNMK_000531"
     tRNA            22098..22174
                     /locus_tag="ACFNMK_000531"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22132..22134,aa:Arg,seq:acg)
     gene            22192..22266
                     /locus_tag="ACFNMK_000532"
     tRNA            22192..22266
                     /locus_tag="ACFNMK_000532"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22225..22227,aa:Glu,seq:ttc)
     gene            22825..24000
                     /locus_tag="ACFNMK_000533"
     CDS             22825..24000
                     /locus_tag="ACFNMK_000533"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC6363858.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATVGR"
     gene            complement(24060..24608)
                     /locus_tag="ACFNMK_000534"
     CDS             complement(24060..24608)
                     /locus_tag="ACFNMK_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC6363859.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(24611..26107)
                     /gene="ccoG"
                     /locus_tag="ACFNMK_000535"
     CDS             complement(24611..26107)
                     /gene="ccoG"
                     /locus_tag="ACFNMK_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC6363860.1"
                     /translation="MTTENQAGNPESGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            26634..28613
                     /gene="tkt"
                     /locus_tag="ACFNMK_000536"
     CDS             26634..28613
                     /gene="tkt"
                     /locus_tag="ACFNMK_000536"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC6363861.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            28754..30142
                     /gene="fumC"
                     /locus_tag="ACFNMK_000537"
     CDS             28754..30142
                     /gene="fumC"
                     /locus_tag="ACFNMK_000537"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC6363862.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSSLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(30207..31130)
                     /locus_tag="ACFNMK_000538"
     CDS             complement(30207..31130)
                     /locus_tag="ACFNMK_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6363863.1"
                     /translation="MENQRPHLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAKPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
BASE COUNT         7778 a         8923 c         7986 g         6614 t
ORIGIN      
        1 ttaatccact atatttctat aataaacttt ctatgggcag cagggatagg atttttgcgg
       61 cgatgcgttt ccaaagtttg gcttcgggtt cgttcgggta ggtttttcgg gtggcgggat
      121 cgtgccattg caggcggttg tgcctgtcga gggtaacgcg gtaggcgtat tcgggtgtgg
      181 tatcggcaag ggtgcgctcc atctgttctg cgattttggg gctttcgata acaacgccca
      241 tttcagtatt gagccgtgcg gaacgggggt cgaggttgaa tgagccgatg aagatgcgtt
      301 tgccgtccac aatgaaggtt ttggcatgca ggctggttac ggagctgccg gtcaggcctt
      361 tgtcttttgt ggcagggacg gcatggttgg gttgcagctc gtagagtttg atgccggctt
      421 tgagcagcgg ttttcggtat ttgacatagc cggaatggac ggcggcaacg tcggtcgcct
      481 gtagcgagtt ggtcaggacg gtaacgtcta tgccgtcctg caccagtttt gccagtgcgt
      541 ctgtgccgga ttttgtgggg acgaaatagg gtgaaaccag atagacgctt ttttcgggct
      601 gtttgagcgc gtcttgcagc cgcccggcaa tcggcggttt gcggcggtcg cggtcgagtc
      661 cttttgcagg gtcgtcgctg atgaggcggg tttggacgct ctgccagtcg atgcgtcctg
      721 cctgtatttt ttggtagagg ggcgactgtt cgacggtttc gcggtagcgc aggagcgcgt
      781 gtctggacgt ttcgtcgttg tatccgagtg cttgaagacc cttgccgatg ttgccgctgc
      841 ggatgatgcg cgtggcgttg tgggcggaat ggcttgccca gtagcggtcg aagtcgtgcg
      901 atacttcgcc gacgacgctg ccggtggcga ggatgtccag gtcggcgaaa acggtgtcct
      961 caccgacttt gaagtattcg tcgccgatat tgcgtccgcc gagtatggtg gcgcggttgt
     1021 cggcggtaaa ggatttgttg tgcatgcggc ggttgaggcg ggggaagtcg gtcaggtagc
     1081 cgagtgcgcg ccattttcgt aagacgaagg ggttgaacag gcgcacttcg atattgggat
     1141 ggctgtcgag ggcgagcagg aggtcgtcca agccgcgcgt gttgttgtcg tccaacagca
     1201 ggcgtacgcg cacgccgcgt tctgcggcaa ggtacacgag gttgaacagc agtcggccgg
     1261 aaatgtcgtt gcgccagatg tagtattgca aatcgaggct gtgttcggca gattcgataa
     1321 gggcggcgcg ggcagcaaag gcttcgtggg ggtcgttcag cagatagata tcggatagcc
     1381 cgttggtatg aggggtgtgc cggatttgca ggatgttgtc caggcggacg ggtttggaag
     1441 tattgaaatg acggctttcc gtccgttctt ccagtggggg caaccatgaa gaacatgaac
     1501 agagaaggag gcataaaagg gaaattaggc tgcgtgtttt catcagggat atggtttcag
     1561 acggcattgc ctgtgttttg gggttggcgc gcatggaagt gcggcatcat aatccaaacg
     1621 ttgaaagggg taaaagtttt gcgtgcggac cgctttagga cggtgtgttc cgtgtcaggt
     1681 ttgcgccgtc tgaaacgtgc agacgcttga aaaccaacga cgaggcaagg gtgatgccgc
     1741 cgatgctgag cagggtcata cggaaggcgg aatgcagacc tgaagaagcc ggtatcagaa
     1801 atgtccagtt tttaaggatt aatgcgccga caacaatgcc catgctgatg gcaagctgtt
     1861 ggttgaccgc catcaggctg ttgccgctgc cggtctgttg cgggcgcaaa tcggcgaggg
     1921 tcagtgtgtt catggcagaa aactgtaggg agttgcacgc gccgatcgcc agcgagagga
     1981 aaatccaaat ccacagcggc gagtttccat caggcagggc gagcagcatg atgaaggcgg
     2041 caagcagttt ggtgttccaa agcagcacct tgcggtagcc gaaacgcttc atcaaggtgg
     2101 agattaacgg cttgaccagc agcgaagaca gggcgacggg tgcgaccagc caacccgaca
     2161 ggcttgcgcc gaagccgaaa gcgacttgaa acatcagggg catcagaaaa ggaatcgagc
     2221 tgatgccgag acggctgaac agattgcccg ccagtcctag acggaaagtg cgtatcagaa
     2281 acaggtcggc ggaataaatc ggtttggacg cggttttcat atgtcggaaa taacggcgtg
     2341 caaacagcag tccgccgcac agcggcaaca gtgcaaaata cggaggcagc gcgtgcgaca
     2401 ggctttctgc cgaaagtaac aagaggcacg cggcggcaga aaaaatcaga tagcctttaa
     2461 aatccagagc ggtatcgttg cctttgacat cgggcatgat gttgcgtccc aatatgaaac
     2521 ccagaagacc gatgggcagg ttgagcagga aaatccagtg ccacgaagcg tattcgacca
     2581 gataaccgcc cgccaaaggc cctaaaaccg gtccgattaa tgcgggcata accgcataat
     2641 tgatggcgtt gagcagcttg gacttgtcgt acacacgcaa gatggtcaga cgcggtatcg
     2701 gaaccagcat cgaaccgccg atgccctgaa cgacacggga aagcgtcaat tcaaacagcg
     2761 aacccgatgc ggcgcacaat gccgatccga gcataaaaac ggcaatcgaa ccgaaaaaga
     2821 cttttttcgt tccgaacctg tccgccaaat aaccgctcaa aggaatcagc agggcaaccg
     2881 tcagcgtgta ggaaataacc gccagctgca tgttcaatgg cgactcattc aggtcggcgg
     2941 caatttcagg cagtgcggta tttaaaatgg tcgcatccaa catctgcata aaaatggcaa
     3001 ttgccagcag aagcggcagc caaggggatg gtgcgcgggc ggatagggtg tttttttcca
     3061 tagagcgatt gtaccccatc cttgtgccgt tattgttttc agatgctgtc tgaatgccgt
     3121 cagagtcggc atcttgaatg ttcacaagca aacgaaccgg cattgcattg taatgataat
     3181 tattatcgaa aactatcaga ttaaggtaca gtaagcgtta tggggtagtt tgtaagaaaa
     3241 accggattat tttttaaaat tagacttgac ccgcaacagt caattactta aagtaaacgc
     3301 ttacctttct acagaaaaaa acgggtttcc cgttatcaaa aaacatgagc gcaaccattc
     3361 ccccaaaaat catccgatac gacagcaatc cgaccgatgt ctattttttc ggcacttgcg
     3421 tccttgatct ttttatgccc gaagcaggca tggatgccat taccctaatc gagcaacagg
     3481 gcatacgcgt ccatttcccg atggcgcaaa gctgctgcgg ccagcccgcc tattcatccg
     3541 gccatccgac cgaagccttc gatgtcgcca gagcgcaact cgaccttttc cccgaggagt
     3601 ggccgattgt cgtaccgtcc ggttcgtgcg gcggtatgat gaagcaccat tggccgacgc
     3661 tgtttaaagg cagcgagtac gaggaaaggg ctgtggattg cgccggccgc atcatcgagt
     3721 ttacccattt cctgcttgcc atcggtttca aacccgaaga caagggcgaa ccgctcaaag
     3781 tcgccgttca cacttcctgc gccgcccgcc gcgaaatgaa tgtccatctt tcaggctggc
     3841 aactgattga cggtatggaa aacgtcgaac gcatcgtcca cgaccacgaa agcgaatgtt
     3901 gcggcttcgg cggcacattc tccgtcaaac aagccgatat ttccggcgca atggtaacag
     3961 acaaagtcgc cgcgctgaaa gaaaccggcg caaccgaaat catcagcgcg gactgcggct
     4021 gtatgatgaa catcggcggc aaaatcgcca aggacgagcc ggatatgccg cgtccgaaac
     4081 atatcgcatc cttcttgttg gaacgcaccg gaggcaaagc atgagcgcgc gcgaaaatat
     4141 tttggcaaaa ctgaaaaaag ccgacgcatt gccgatggaa gaacctgcgg tttttgatta
     4201 ttaccgtgaa atgggtgttt cttggggcag cgaagttgag cgtctgaaac attgggctgc
     4261 cgcaatgcgc gcggtcaaaa ccgaaattta ttgggtgacg aaaagcaatt ggatgcaggt
     4321 tttccgcgaa gcggcagaag gcaagggttt gaaaaacatc ctgctgccct tggcgaccga
     4381 acacggacaa attgcccgtg ccgcattggc ggacagcaat atcgaaccga ttgccttcga
     4441 gcgcgaaatc gatacttgga aaaccgagtt tttcacgaac atcgatgcgg gcttcagcgg
     4501 cgcgcaatgc ggcatcgccc gcaccggcac gctgatgctg ttttccagcc ccgaagaacc
     4561 gcgtactttg agtctcgttc cgcccgtgca tttctgcctg ttcgatacgt ccaagatgta
     4621 caacgagttt cataatgccg tcgaaggcga aaaactggtg gaaaacggta tgccgaccaa
     4681 tgtattcctg atttccggcc cgtccaaaac cgcagacatc caactgacgc ttgcttacgg
     4741 cgcgcacggc ccgcgcgatt tggtcatcct cgccatcctg cccgaccaca tttcccctgc
     4801 cgatttggag gaaaacgcat gactacgcaa accatcaaat ttcacatgaa gccggaaact
     4861 ttcaagcaaa acgccgcaat ttcccttcaa gacaagcctt tgcgcaaaag cctgcgtacc
     4921 gcgatggata tgctgatgac caaacgcaaa gccgttttga ccgacgaaga agagctgcaa
     4981 agcctgcgcg atttgtgcga acacgtccgt cagcgctcat tgtctaaatt gccagccctg
     5041 ctggagcagc tggaagaaaa cctgactaag ttgggcgtga aagtgcactg ggcagaaacc
     5101 ccgaccgaag cctgccaaat tatccacgac atcatcacag ccaaaaacgg caagctgatg
     5161 gtcaaaggca aatcgatggt cagcgaggaa atcgagctga accattatct tgaagcaaaa
     5221 ggcattaaag cggtagaaag cgacttgggc gagttcatcg tccaaatggc aggcgaaaaa
     5281 ccgacccata tcgtgatgcc tgcaatccat aaaaccaaag aacaggtgag cgaactgttc
     5341 caccaaaacc tcggcacacc gcttacagac gatgtggacc aactgaccgg cttcgcccgc
     5401 aaagccctgc gcgatattta cagcactgcc gatgtcggct tgagtggcgt aaactttgcc
     5461 gttgctgaaa caggtacgct gtgtctggtg gaaaacgagg gcaacggtcg cttgagtacc
     5521 accgttccgc ccgtgcatat cgctattacc ggcattgaaa aagtggtggc gaaattgtcc
     5581 gacatcccac ccttgtacag tctgctgccg cgctccgcca tcggtcagaa catcacaacc
     5641 tatttcaata tgattaccgg cccgcgccgc agtgaagaat tagacggtcc gcaagaaatg
     5701 cacttggttc tgctcgacaa cggccgcagc caggcttatg ccgaagacca aatgcgccgc
     5761 accctgcaat gtatccgttg cggcgcgtgt atgaaccatt gcccggttta tacccgcatc
     5821 ggcggcgcgg catacggcac aacctatccc ggcccgattg gcgagattat ttcccctcac
     5881 ctgttgggtc tggatgccac ccgcgacctg ccgaccgcct gtaccatgtg cggtgcgtgt
     5941 gtggaagtct gtccggtacg catcccgatt accgaacaaa tgcagcgttt gcgcgttgaa
     6001 gcgcaacgtt cgccgaccga aaccgtgccg caccccatcc gagggcaagg cgcatcgcat
     6061 accttcggcg aacaaatggc gtggcgcaca ttcaacggta ttttcagcgg cagcaaaacc
     6121 taccgcgcct tcggttgggc agccaccaag ttccgcaacc tgaccccgcg caaacagttg
     6181 ggttggacgc aaaaccgcgt gccgatgaaa ccggcgaaga aaaccctgca cgaactaatg
     6241 gcagaaaaaa tgcgccaaaa agaacaggca taaaaagttg ttcgcaaaaa tgccgtctga
     6301 aacccgaaac agggcttcag acggcatttg tatagtggat taacaaaaat caggacaagg
     6361 cgacgaagcc gcagacagta caaatagtac ggcaaggcga gggaacgccg tactggttta
     6421 aatttaagcc actatatatt cgcggacggc gggttttaaa tctgttccaa ttccatactc
     6481 aaaacagcct gttcctgttt ggctcggaag tctgccagtt tttgcgccag ttcgggggtt
     6541 tcgttggcga gcatggaaac ggcgaacaat gcggcatttg ccgcgcctgc ctcgccgatg
     6601 gcgaatgtgg cgacgggtac gcctttgggc atttgtacaa tcgataaaag cgaatcttcg
     6661 ccgcgcaggt atttgctggg gacgggtacg cccaaaacgg agacggtggt cttggcggca
     6721 accataccgg gtaaatgcgc cgcgccgccc gcacccgcga tgatggcttt gatgccgcgc
     6781 gcccgtgcgg tttcggcgta ttggaacatt aaatccgggg tgcggtgtgc ggaaacgacg
     6841 cacgcctcat attctacgcc gaactcttca agaaactgcg ctgcctgccg cataacgggc
     6901 caatcgctgt tgctgcccat gatgatgccg atttgaatca taaatcctcc ttggtgcgga
     6961 tggggtaaaa agcggaaaaa tggaaaaact atcgtttgcg tacggctgcg gcggcgcgtt
     7021 ttgccgccgg gctgctcggg taagcctgta tcaggctgcg ccaagtcgcc cttgcaatgt
     7081 ctttctgctg caacctgtat tggcattcgc cgattttgaa catggcttca ggcgcggttg
     7141 ggctgtcttt gaaacggttg gcgtaacgcc ctccgatttc gatgacggat tcgcagttcc
     7201 ccatacgcgc cctgctttgc agcaacaggt acatactgcg ttgcgcgata ctgccgccgt
     7261 cgcctccgtc cgcgcctttc aacagggagg cagcggcaga aaacctgccg cttttatagt
     7321 gtttgagtgc ctgattgtag aggttttgtg cggtttcgac ggtatgtgcg gatgcgctgc
     7381 cgccttcggt attgaggtaa tgctctttca acttgcggtc gtcgagtttt tggacgtatg
     7441 ccctgctgga agggtgtgtt ttcgcatgct ccagtgcttt gactttgccg tttaaggttt
     7501 ccacttcgtt cgacagccgg acgattctgc cttccagata gtccaaacgg tcttgcaagg
     7561 tcggaacggg atagggaata gcgtccgaac tacctgcctc tgcctgcggt tcgagccgca
     7621 tatcctgaat attgcgggaa acaggggaag aacaggcggc ggatacggac agccaaatga
     7681 taaaaagcgg taatttggtc ttcattattt tttcagaagc agggtcaaac cgtcgccgac
     7741 gggcagggtg atggggacaa tccgcgtatc gttcggcagg ttttgattaa aatctttgag
     7801 gatgccgacg ctgggcggcg catcggaagc cgcttcgcgc atcacccttc cgttcaacaa
     7861 aatattgtcg atggcaatga tgccgccttg ccggacaagc ttgaggcaac gctcgaaata
     7921 ttgcggcgtg ggcggtttgt ctgcgtctat cagtgccaaa tcgtagcttc cggcttcacc
     7981 ctgtgcaatc aaatcatcca atgtcagcaa tgcgggttgc agatgcaggc tgattttatg
     8041 tgccacgcct gcctcgttcc aaacctgacg cgccgtatcg gtaaaggtta cattgatgtc
     8101 gcaggcggta atccgcccgt gttttggcaa cgccaatgca agcgcggtgc tgctgtatcc
     8161 ggtaaatacg ccgacttcca gatatttttc cgcgcgaatc agctttgcca gccaaaccaa
     8221 aaccgccgcc tgttcgcgcg caatcgccat tttgcccata cggtgatgcc cggtcttctc
     8281 acgcagccgc gtcaaaacgg gatgttcggg ttcgccgatg gcgttcaaat agttttgcag
     8341 gtcgggggcg acattggaca gatgggtcgt catttcggcg ggttcagtct tggtaatagg
     8401 tataaggttt tttcgctact tttgccgcct cgaagttttc ctgttcttcg ggattgagtt
     8461 cgacatccca caaaagcccc cggttttcca aacgctgctg ttccaactca ggtttttctt
     8521 caatcaggcg gttgaggaat tgtgtggctt cggattggta gtgatacatc tttgcgctcc
     8581 aattttacgg gatacggcgt gattatactg gtattttcca aacgggataa acggctttta
     8641 tcaagaatac aggcagaaaa gtaaggggtt ttattataga ataaagcgtt tttgcaacgg
     8701 aagcctacct tatgccacgc attgccgccc tgcccgacca tctcgtcaac caaatcgccg
     8761 ccggcgaagt ggtcgaacgc cctgccaacg ccctgaaaga aatcgttgaa aacagcatag
     8821 acgcaggcgc gacggcgatt gatgtcgaac tggcgggcgg cggcatccgt ctcatccgcg
     8881 tcagcgacaa cggcagcggt atccatccgg acgacatcaa actcgcactc caccgccacg
     8941 ctaccagcaa aatcaaaacc ttaaacgatt tggaacacgt cgccagtatg ggctttcgcg
     9001 gcgaaggctt ggcaagcatc gcctccgtca gccgcctgac cctgaccagc cgccaagacg
     9061 gcagcgcaca cgcgacacaa gtcaaagccg aagacggcaa actcagcagc cctacggcag
     9121 ccgcccaccc cgtcggcacg accatcgaag ccgccgaact cttcttcaat acccctgcgc
     9181 ggcgcaagtt cctcaagtct gaagccaccg aatacgccca ctgtgccacg atgctcgaac
     9241 gcctcgcgct ggcgcatccg cacattgcct tctcgctcaa acgcgacggc aaacaagtgt
     9301 tcaaacttcc tgcccaaagc ctgcatgaac gtattgccgc cattgtcggc gatgattttc
     9361 agacggcatc cttagaaatc gacagcggca acggcgcatt gcggctctat ggcgcgattg
     9421 ccaagccgac tttcgccaaa ggcaaaaccg acaaacaata ctgcttcgtc aaccatcgtt
     9481 ttgtccgcga caaagtcatg ctccatgctg tcaaacaggc ataccgcgac gtattgcaca
     9541 acgcgctgac gcctgctttc gtcctctttc tcgacctgcc gcccgaagcc gtggatgtca
     9601 acgtccaccc taccaaaacc gaaatccgct tccgcgacag ccagcaggtg caccaactcg
     9661 tgttccacac gctcaacaaa gcccttgccg acacacgcgc cgacctgacc gaaagcgtcg
     9721 gcaacgcagg cgaagtgttg catgacatta ctggcgttac gcctgcacca atgccgtctg
     9781 aaaacaacag cgaaaatctg tttgatcgcg catccaacta cccgacgggc aacaaacccg
     9841 atacacgcaa tgcctttggt gcatcaggca aaaccgcgcc tatgccttac caagccgctc
     9901 gtgcgccgca acaacgcagc ctgtccttgc gcgaaagccg cgcagccatg aatacttacg
     9961 ccgagcttta caaaaaaacc gacgacatcg accttgagtt gaaccgatta gagcaggcac
    10021 gtttcggcaa tatgccgtct gaaacgccta ctcccaaaac agatgcgccg ctttcagacg
    10081 gcatcccgtc ccaatccgaa ctgccgccgc tcggcttcgc cattgctcaa ttacttggca
    10141 tctacatcct tgcccaagcc gaagacagtc tgttgctcat cgatatgcac gctgccgccg
    10201 aacgcgtcaa ctacgaaaaa atgaaacgcc aacgtcaaga aaacggcaat ctgcaaagcc
    10261 agcgcctgct tattcccgta acctttgccg cgtcccacga agaatgcgcc gcccttgccg
    10321 attatgccga aacgctggca ggcttcgggc tggaattatc cgatatgggc ggcaacaccc
    10381 tcgccgtccg tgcagttccc gccatgctcg gcaaagccga tgtcgtctcg ctcgccaaag
    10441 acgtattaaa cgaactcgcc caagtcggca gcagccaaac catcgaggaa cacgaaaacc
    10501 gcatcctcgc caccatgtcc tgccacggct cgatccgcgc cggccgccgg ctcaccctgc
    10561 ccgaaatgaa cgcccttctg cgcgatatgg aaaatacgcc gcgcagcaac cagtgcaacc
    10621 acggcaggcc gacttgggtc aaactgactt tgaaagaatt ggacgcactg ttcttgcgcg
    10681 gacagtaagc cgaaagtgct agaatacgcc gcccgagacc gccgttcaga cggcattccg
    10741 acgcaccgac agaaacatca cgaccgaaac caagagaaaa acatggccta tcaagttctc
    10801 gcccgaaaat ggcggcccaa aaccttttcc gacttagtcg gtcaggaaca cgtcgtcaaa
    10861 gccctgcaaa acgctctaga cgaaggcagg ctgcaccacg cctacctgct gaccggtacg
    10921 cgcggcgtag gcaaaaccac cattgcccgc atccttgcca aaagcctcaa ctgcgaaaac
    10981 gcgcaacacg gcgaaccttg cggcgtgtgc gaaagctgca cccaaatcga tgccggacgc
    11041 tatgtcgacc tgctcgaaat cgacgccgcc tccaacacag gcatcgacaa catccgcgaa
    11101 gtcttggaaa acgcccaata cgcgccgacc gccggcaaat acaaagtcta tatcatcgac
    11161 gaagtgcata tgctttccaa aagcgcgttc aacgctatgc tcaaaacgct ggaagagccg
    11221 cccgaacacg tcaaattcat ccttgccacg accgatccgc acaaagtccc cgttaccgtc
    11281 ttgagccgct gcctgcaatt cgtcttgcgc aatatgactt cgcagcaggt tgccgaccac
    11341 ctcgcccacg tcctcgacag cgaaaaaatc gcctacgaac ccgtcgccct gcaacttttg
    11401 ggacgcgccg ccgccggatc gatgcgcgat gccttgagcc tgctcgacca agccatcgcc
    11461 ctaggttcgg gcaaagttgc cgaaaacgat gtccgccaaa tgatcggcgc ggttgacaaa
    11521 caataccttt acgaactctt gacaggcatc atcaaccaag acggcgcagc cctgctctcc
    11581 aaagcgcagg aaatggcggc gtgtgccgtc ggctttgaca acgccttggg cgaacttgcc
    11641 atactgctgc aacacctcgc cctgatacag gcagtgccga atgccttggc gcacgacgac
    11701 cccgattccg atattttgca ccgcctcgcc caaaccatag gtggcgaaca aatccagctt
    11761 tactaccaaa tcgccgtcca cggcaaacgc gacctgagcc tcgccaccga cgaatacgcc
    11821 ggctttatga tgaccctgct gcgtatgctg gcgtttgcgc ccttggcggc agcttcgtgt
    11881 gatgcaaacg ccgtgattga aaataccgaa cttcaatccc catcggcaca aaccgccgaa
    11941 aaggaaacct ccgcaaaaaa gccccaaccg cgccctgaag cggaaaccgc gcaaacatcc
    12001 gttcagacgg catccgcagc ggcaatgccg tctgaaagca aaaccgccga acccgttacc
    12061 aatcaagaaa acaacgatgt tccgccttgg gaagacgcgc cggacgaagc ccaaaccgca
    12121 gcctgcacgg cgcaagcatc ggcaaaaagc attcagacgg catccgaagc cgaaacgccg
    12181 cccgaaaacc aagtttccaa gaacaaggca gccgacaacg taaccgatgc ccccttgtcc
    12241 gaagtgccgt ctgaaaaccc cattcaggca acaccgaatg atgaagccgt tgaaacggaa
    12301 acatttgcac acgaagcccc tgcagaacct ttctacggtt acggctttcc ggataatgac
    12361 tgccccgtag aagacggcgc agaaatcccg ccgcccgatt gggaacacgc cgcccctgcc
    12421 gataccgcag gcggaggcgc ggacgaagaa gcagaagcag gcggcatcgg cggaaacaac
    12481 acgccgtccg ccccgccgcc cgaattttcc accgaaaact gggcagccat cgtccggcat
    12541 ttcgcccgca aactcggcgc ggcgcaaatg ccggcgcaac attccgcgtg gacggaatac
    12601 catcccgaca ccggcctgat ggttttggca atgaccgccg aagcacgcgc caccgccgac
    12661 aaaaaacgtc tcgacaaaat ccgcgacacc cttgcccaag cctacgggct gcaactcacc
    12721 ctgcaaaccc aagactggca cgacgaagcc ggcagagaaa cccccgcgat gcaggacaag
    12781 cgcgtccaag ccgaagacag gcaaaaagca caagcattgc tcgaagccga ccccgccgca
    12841 caaaaaatcc tccaagcatt cggcgcgcaa tggcagcccg aatcactgga attagcggca
    12901 aaccggccat aaacagatat aatgccgccc aaacctttcg gacggcattg ccgtttcccc
    12961 attcattcaa acacagacag gagttttacc tatgttcgga aaagccggat taggcggcct
    13021 gatgaaacag gcgcagcaaa tgcaggaaaa tatgaaaaaa gcgcaagcca aacttgccga
    13081 aaccgaaatc gaaggcgaag caggcaacgg cctggtcaaa atcacaatga cctgcgcgca
    13141 cgaagtacgc aaaatcgaca tcagccccga tttgattcaa gaagccgccg acgacaaaga
    13201 aatgctcgaa gacctcatcc tcgccgccct caaatccgcc cgaggcaaag ccgaagaaac
    13261 cgcaaacaaa acaatgggcg cattcacgca aggtctaccc cccggagtgg gcgacttctt
    13321 ccgctgatac ccgaccgtca ttcccacgca ggcgggaatc tagaacgtag aatctaagaa
    13381 accgttttac tcgataaatt tccgtgccga cgggtctaga ttcccgcctt cgcgggaatg
    13441 acggcatcag tttgcaggat tcggcgtgaa cggtaaaaac agtgagaatg ataagaacgc
    13501 aaaaacggca agaatagcgg gaatcggcag gctgaagccc accctaccat tatttacaca
    13561 tccgtaccgc ttaaatgccg tctgaaactt cgtcattccc gtgaaaacgg gaatccagcc
    13621 ccgtgggagc agaaacttac accccgtcat tcccgcgaac gcgggaatcc agtaaccgaa
    13681 aaaccacagg aatctatcgg aaaaacagaa acccccgacc gtcattcccg cgaacgcggg
    13741 aatccagtaa ccgaaaaacc acgggaatct atcggaaaaa acagaaaccc tcgaccgtca
    13801 ttcccgcgaa cgcgggaatc tagaacgtag aatctgagaa accgttttac tcgataaatt
    13861 tccatgccaa caggtctgga ttcccgcctt cgcgggaatg acggcatatt ttttgcattt
    13921 gatataaagg gtcgtttgaa ttttgttcag caagtgcaaa gtgttgcaca taaaagggcg
    13981 taggcttcat ccgtaaagtc ggcgcaggat agaggcaaag cgggcgtagg tcgggctgta
    14041 gcaactgtat ttttcacccc gtcgggcaaa aataccaaaa ctcaaatcaa gccgttcgga
    14101 ggcagctcaa aaaacggtac ttcgcaacag aagtaccgtt tatcgggatt ttaagtttta
    14161 ttcttctggg cgttcgccgt cggtttcgtc caattttcct tcggtgatgt gcatttctac
    14221 gccgttgagg gcgcgaattt ttgcgtcgat ttcgtcggca atttcgggat tttccttcag
    14281 ccagacgcgg acgttgtctt tgccctgacc gattttcgcg ccgttgtagc tgtaccacgc
    14341 gccggatttg ttgatgatgt cgtttttcac gccgatgtcg atcaattcgc cctcccaact
    14401 gatgccttct ccgtagagga tgtcaaactc tgcctgacgg aacggggggg cgactttgtt
    14461 tttgatgact ttgacgcggg tttcgttgcc caatacttct tcgccttttt tgatggatcc
    14521 ggtgcggcgg atgtcgaggc ggacggaaga atagaatttc agcgcgttgc cgccggtggt
    14581 ggtttcgggg ctgccgaaca ttacgccgat cttcatccgg atttggttga tgaacacaac
    14641 cagcgtgttg gtttttttga tgtgtccggt cagtttgcgc aaagcctgac tcatcaggcg
    14701 cgcctgcagt ccgacgtggc tgtcgcccat atcgccttcg atttcggctt tggggacgag
    14761 tgcggcgacg gaatcaacga ctaccatatc tatgccgccc gaacggacaa gtgtgtcgca
    14821 gatttccaaa gcctgttcgc cggtatcggg ctgggacagg taaagctctt cgacttttac
    14881 gccgagtttg cgggcgtaaa cgggatcaaa ggcgtgttcg gcatcgacaa aggcgcacac
    14941 gccgccgttt ttctggcatt gtgcgacggc ttccaagcat aatgtggttt tgccggaaga
    15001 ttcgggaccg aagatttcga cgatgcgccc gcgcggcaga ccgccgactc cgagtgcgag
    15061 gtctaatccg agcgatccgg tggaaatgac ttcgaggttt tcttcctgct ggctgccgtc
    15121 cattttcatg atggcgcctt tgccgaaact tttttcgatt tgcgccagtg cggcggcaag
    15181 ggctttgctt ttgtcgtctg acattggggt tactccggaa caaatgcggt atgtgggatg
    15241 cggcgcaaca cgggctgcgg cgcgggattt gtatcgtttt cccgatgtgc gggctatcgg
    15301 taatgctgct tcacggggtt gccattatcg catatttcct tgcttgccga tatgcggcag
    15361 gacgcggcgg cttgtgccgg aatggaatct ggatgccgtc taaaaggcgg ccggctttgt
    15421 tataatggcg gctgtttttt ctgtgtgtgc ctgttttatg tgttcctgcc ttgttgtcaa
    15481 aaataccgtt atcggaagcg gacgcaccaa aatcgccgtg ccgcttgtcg cccgcgatgc
    15541 cgccgtactt tccgccgtac ttgaccaaat caaaaacctg cccttcgata tcgtcgagtt
    15601 ccgcgccgac tttttggaat gcgcgggcag catcggcgaa gtattgcgcc acacgcagac
    15661 cgtccgcgac gcgctgcccg acaagccgct gctgtttacg ttcagacggc atggcgaagg
    15721 cggcgcgttc ccgtgttcgg acggttatta ttttgaactg ctcgacgcgc tgatcgaaag
    15781 ccgcctgccc gacatcatcg acatcgagct gtttgccggc gaaaccgccg tccggcgcgc
    15841 cgtggcggat gctcaaaaaa acggcatcgc cgccctgctc tgcaatcatg agtttcaccg
    15901 cacgccgccg caagaagaaa tcgtatgccg tctgaaacag atggaggact gcggcgcgga
    15961 catctgcaaa attgcggtga tgccgcaaag cgcggaagat gtgctgactt tgctttccgc
    16021 cacgctcgaa gcgaaacggc ttgccgccaa accggttgtt acgatgtcga tggggcagac
    16081 gggtgcggtc agccgccttg ccggacaggt gttcggctca agcatcacgt tcggttcggg
    16141 aacgcaaaac tccgcgccgg ggcaaatcgg cgtatccgcc ctccgtgcga cactcgactg
    16201 cctcgaaaac ggcgcagact gatttcagac agcatcaaaa catgatgaaa ctcaatcccc
    16261 aacagctcga agccgtccgc tacctcggcg gcccactgct cgtccttgcc ggtgcaggca
    16321 gcggcaaaac cggcgtgatt actcaaaaaa ttaagcattt gattgtcaat gtcggctacc
    16381 tgccgcatac cgttgccgca attaccttta ccaacaaagc cgctgcggaa atgcaggagc
    16441 gcgttgccaa aatgctgccc aaaccgcaaa cgcgcgggct gacgatttgc acgttccact
    16501 ctttgggcat gaagattctg cgcgaagagg cgaaccatat cggttacaaa aaaaacttct
    16561 ccattctcga ttctaccgac agcgcgaaaa tcatcggcga actcttaggc ggtacgggca
    16621 aagaagccgt attcaaggcg cagcaccaga tttccttgtg gaaaaacgat ttaaaaacgc
    16681 ctgaagatgt cgttcagacg gcatcgaaca tttgggaaca acaaaccgca cgcgtgtatg
    16741 cgagctatca ggaaacctta caaagctatc aggcagtgga cttcgacgac ttgatccgcc
    16801 tgcctgccgt gctgttgcag caaaacagcg aagtgcgcaa caaatggcag cggcggctgc
    16861 gttatctgct ggttgacgaa tgccaagata cgaatacctg ccaatttacg ttgatgaaac
    16921 ttctgaccgg cgcggaaggt atgtttaccg ccgtcggcga cgacgaccag tccatctacg
    16981 catggcgcgg cgcgaacatg gaaaacctgc gcaaaatgca ggaaaactat ccgcagatga
    17041 aggtcatcaa actggagcaa aactaccgct ccaccgcgcg gattctcaaa atcgccaaca
    17101 aagtcatcga gaacaacccc aagctgttca ccaaaaaact ttggtcgaaa ttgggcgaag
    17161 gcgagcccgt caaagtcgtt gcctgccaaa acgagcagca cgaagccgac tgggtcgtca
    17221 gccaaatcgt caaacaaaaa ctcatcggcg gcgacaaaac ccaatatgcc gatttcgccg
    17281 tgttataccg cggaaagcat caggcgagga ttttcgagga agcattgcgc ggtgcgcgca
    17341 tcccctacca gctctccggc ggacaaagct ttttcgacaa agccgaaatc aaagacgtgt
    17401 tgtcttatgt gcggctgctt gccaacccca atgacgatcc cgcctttctg cgtgccgtta
    17461 ccacgcccaa acgcggcatc ggcgatgtca cgctgggcaa gctcaacact tacgcgcacg
    17521 aacacgaatg cagcctgtat gaagccgcgc aaaacgaaga agcccttgcc acgctgaaca
    17581 ataccaaccg ccaacacctg caaaccttta tggatatgtt cgtcagctac ctcgccaaag
    17641 ccgaaaccag cgaagcgggc gagttcatca acagcctgct cgaagaaatc gactatgaaa
    17701 accatttgat gcaaaacgaa gaaggcaaag ccggcgaaat caaatggcgc aacgtcggcg
    17761 atttggtatc atggtttgcg cgaaaaggcg gggaagacgg caaaaacatc atcgaactcg
    17821 cccaaaccgt cgccttgatg acgcttttgg aaggaaaaga cgaagaagaa accgatgccg
    17881 tctcgctatc cacgctacac gccgccaaag gtttggagta tccgtatgtt ttccttgtcg
    17941 gttgcgaaga aggcgttttg ccgcacaacg acagtatcga agagggcaac gtcgaagaag
    18001 aacgccgcct gatgtacgtc ggcatcaccc gcgccaaacg ccaactcaca ctgacccact
    18061 gcgtcaaacg caaaaaacaa ggcacatggc agttccccga acccagccga ttcatagacg
    18121 aaatgccgca ggaagatttg aaaatcctgg ggcgcaaagg cggcgaaccg attgtcagca
    18181 aagaagaagg cagacgcaac cttgccgata taatcggaag gctcgacaac ctaaaaaaaa
    18241 gcggcgcggc ggattaaacc ggagccgcaa tgccgtctga aggcttcaga cggcatgttt
    18301 tttggacggc gtggtaaatc ggtttacgcc cacaagccct gctgctggtt tttcggcaca
    18361 agatgtccca cgccgatgcc gataaggcgg aacgcgtctt ccgtctgcgg cgggacgcgc
    18421 gccatcaaca tttgcgcagc ctgcagcaga gctgcgcagt cgggcaatac ggaggaataa
    18481 gtcagtgtgc gcgtgatgat gcggaaatcg taggtcttca gcttgagcgt tacgctttgg
    18541 gcttcgacgt ttttgcgcgt gatttgccgc cacaagtctt cggcaagatg ggggaggtgt
    18601 ccggcagcct gctcgagcgg caggtcttcg ggcagggtaa tttctgtgga gatttggagg
    18661 cgttcgcgtt cggctttgac ggggcgttcg tccgtaccgc gcaccaaatc atagaggcgg
    18721 tatccgtagc gtccgaaatg gtttaagagt tcgccgcgct cgaaacggcg caagtcgccc
    18781 gccgtccgca tacccagcga ctgcattttt ttcagcgtta ccttgcccac gccggggatt
    18841 ttgcccaaag gcagggtttc caaaaatgcc atgactttgt gcggcggcaa cacaaactgc
    18901 ccgttcggct tgcgccagtc cgacgcgatt ttcgccagaa atttgttcgg cgcgatgcct
    18961 gcggatgcag tcaaacctgt ttccgcaaaa atggcggcac ggatttcttt ggcaacttca
    19021 ctggcgtaag ggatgttttt gaaattacgg gtaacgtcaa gataggcttc gtccagcgac
    19081 aagggttcga ttaaatcggt ataacgcctg aatacggcgt gaatctgcgc ggaaacctga
    19141 cggtacaaat cgaaatgcgg cggcacatac accgcttgcg gacacagcct tttcgccgtt
    19201 gccaccgaca tcgcggaatg cagcccgaac tgccgtgcct catacgatgc ggcgcaaatc
    19261 accgaacgcg cgccctccca cgcgacgacc accggccgcc ctttcaaatg cggctgttcg
    19321 cgcagctcta ccgatgcgta gaatgcgtcc atgtcgatgt ggataatttt gcgtgaagac
    19381 atcggctctt ctgaggataa aagggatatt ctactgccgg catcgggcaa attccaaata
    19441 tacgccccga tatgggaagc cccgcatccg cagcgttgga aacaggcaaa cgcctgcaaa
    19501 caaagccggc aaacggataa cggcatattg cctgattgtc attcagacat aggaaatgcg
    19561 gaaaaaaaca cccaaattgg ctataatccc gacaaacaca ctcaaggaca acaacatggc
    19621 agcctcgccc gaagcaaaat tcactgaaga aaagattttg tgggtcaaac accacacgcc
    19681 gaaactcatc actttcgcca tcagccgtcc cgaatcctac cgttttaaag ccggacagtt
    19741 ctcccgactc ggtttctacg aaggggaagg ctttatttgg cgtgcctact ccatcgtttc
    19801 cgcagaatat gcagacacgc tcgaatattt tgccgtactc atccaagacg gccccatgtc
    19861 ggcccgtttc gccaaaatgc aacagggcga caccatcctg ctcgataaaa atgccaccgg
    19921 cttcctcctg cccgaacgct tccccgacgg caaggatttg gtgatgctct gcaccggctc
    19981 cggcatcgcc cccttccttt ccattctcga acaacctgaa atccgacagc gtttcgatac
    20041 cgtcaacctg atacattccg tatcttttcc cgaagaattg attttcaacg accggctcgc
    20101 cgcattgtcc gaacatcccc tggtaggcga atacggacac tcgttccatt tcgtccctgt
    20161 taccacccgt gccgccaacc cctcgggctt aagcggaaaa cgcattccgg aactcttaaa
    20221 aaacagcagc atcgaacagg cgctgcatac caagttcacc ccggaatcca cacggtttat
    20281 gatttgcggc aacccggaaa tggtcaaaga cactttccaa acgctgctcg acatgggtta
    20341 cgccatgcac cgcaaccgca ttcccggtca aatcatgatg gaaaacggct tctaaaaacc
    20401 accctgcttg tccgatgcct cggacgggca aaccggcacg acacgcaaac cgcgtcggca
    20461 aaaatgccgt ctgaagcctt cagacggcat tttcggatac atacaaacgg caacatattc
    20521 caacccaaac cgattaggca atcaatacga acggctgttt acatacttac cggctttcac
    20581 caaccgatat cgatttaacc gatttcctta atatttttcc tgtccgtttt aaacttcgcc
    20641 ttaaacacat ccggcaaatc tttatcgaaa taccaaagcc cgccatccat ttccaatgcg
    20701 ccgcccattc catgcagaac gactttttcc cgcgttaaag gatcggtttc ggtaatttcc
    20761 acctcatgca tttttcccca atccaaaaca ggcaacttgt gtgcaaacgc caaaacctgt
    20821 tcgtcagaac ggccacacgc cttatcgcat cggcctgaaa catatacggg acaaggctca
    20881 tcctctgcat aaccgtccgg aaggataccg gctgcggcag gacacaatcc gtatgtttcc
    20941 gcccacaacg acaaagcccg tctcgctgcc tttttattga caaataatcc ggtaggcgga
    21001 ttgtccgcca caccgttttt caaagccgct gttttcgcat ccaacatgcc gtctgaacct
    21061 ttttcaaacc tgacggtcgt aaatgtttta agcagatttt tggcagatac gtaacaatcc
    21121 gccccataat gcctgaccaa ttccgcttta accttatggg catgcagcct gcccaatgcg
    21181 ggaaaaaaac ggactgcctc cgtattgcac caatcaaacg aggcttttcc ggaatccaat
    21241 aaagccgaaa cctcgctata tacccgttca aacgtaccga tataatttat tgctcccccg
    21301 ccgtcgaaac aggcaagcac ccccataccg tcaggcaaac cgtacaactg ttccctcaac
    21361 cgttcgggca gcgcggcagg cagcggtttc ggattcatca aacggaaaca ctgcctgatc
    21421 cactcctcga ccccgtgttc cgacagactg tattccaaat aatcacacaa tgccgataca
    21481 tccgccatcg cacgatgcct gtcttccaca acaatcccca acctttcgat gatactgtct
    21541 aggctgtgct tgtaaaattg cggatacaga caccgggaca gctgcacact gcacaaagca
    21601 ggcgatgaaa atccgatacc cgcacgatga aactcatgct ttaaaaacgt atagtcgaaa
    21661 cggctgttat gtgcaaccag cacacaaccc ttcaataccg aaaacaactc gccggcaatc
    21721 tctgcaaaaa caggcgcatc ggcaaccatg ccgtctgaaa tccccgtcag ccccgccaca
    21781 aactgcggaa tcggtttttg aggattaacc aaccactcat gcctcaccac ccttccctgc
    21841 tcaaacttga ccaaagccac ttcggttacc ctgtcttcat acagattgcc gcccgtcgat
    21901 tccaaatcga ccacggcaac cggcattcca aaccgtaaaa acgccttttc cagcaagggc
    21961 caacgggaag caacaatcat tttattctct ttaaattcaa acaacaaacc gatattttac
    22021 acttttaagg catttcatcc aacaaaacaa ttgacagaat ccgatgatta ccctaaaatt
    22081 caaatctttc ttgcagcgca cccgtagctc agttggatag agtatctggc tacgaaccag
    22141 agggtcgggc gttcgaatcg ctccgggtgc gccagtaaga aaatacaata tgcgcccatc
    22201 gtctagcggt taggacatcg ccctttcacg gcggtaaccg gggttcgatt ccccgtgggc
    22261 gtgccaaatt ccaaatcccc gagattatcc ctcggggatt ttttattgtc tcggcaactc
    22321 gttaccatat ctttacctac ccccttcatc agaatctcag acacaatcga atcatattca
    22381 aacctttgcc gtgcaaaccg atatcccata accggatgcg gtgtccgtcc aacattttac
    22441 ccgattgaaa cgcctgatat attgcacccc atcaacgtgg cattactttt cttaacaatc
    22501 ccctttgaca gcaactgact agggcttttt tatgccatca tcaaatttat agtggattaa
    22561 ctttaaacca gtacggcgtt gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg
    22621 tcgccttgtc ctgatttttg ttaattcact ataatatttt ctctcccgat tgaaacaggc
    22681 gtaacagaat atgcccgaag ctccggctgc tttcttgttt accgccgcga tatttagagt
    22741 ataataccaa atttgagcaa tagttctaaa acagttagaa ccatttttca tgagcctgac
    22801 tgattcgtat actcggagaa actgatgcaa aatatttttg accctttggt tattcgtgga
    22861 aaatccctta cccccatcgt gcaaggcggt atgggggtcg gtgtttccgc atcgggttta
    22921 tccagcgcgg tggcacgtga aaacggtatc ggaacgattg ccagtgtgga tttgcgccac
    22981 cttcacgaag acctactcgc cgaatcacaa atcaatccga gtgaagagaa atatacatct
    23041 ttgaactgta ccgcattaga cagggaaatc caaaaagcca aaagcgcttc agagggaaaa
    23101 ggactgattg cggtcaacgt gatgaaggca gtcaaagacc acgccgcgta tgtccgccag
    23161 gcttgcgaat caggggcgga tgcggtcgtg atgggcgcag gtctgccttt agacctgccg
    23221 gaaatgaccg agggctatca taaagatgtc gcgctgctgc cgattctgtc cgaatcgcgc
    23281 ggtattaata tcgtcttgaa acgttggatg aaaaaaggca tattgcccga tgcgattgta
    23341 gtcgaacatc ctgcccacgc ggccggacat ttgggtgcat caaccgttga aggcgtaaac
    23401 gatgccaagt tcgacttcaa acgcgtgatt gaggaaacgt ttgaagtttt caaaagttta
    23461 gggctggaaa gcgaaaaaat cccgcttatt cttgcgggag gcatggcaaa ttttgaaaaa
    23521 gtcaaaaccg ccctaaagaa ctggggagcc tctgccgttc aaatcggtac ggcttttgcc
    23581 gttaccgaag aaggagatgc acaccttaac ttcaaaaaaa cgctcgccgg tgcggaaact
    23641 gaaaaagtag tcgaatttat gtctgttgcc ggtttgccgg cgcgcggtgt ccgcaccaaa
    23701 ttcctagaca gctacatcaa gcgtgaaagc aaacttcaga caaacgccaa agccgacccg
    23761 cgccgctgta cccaaggttt aaactgccta accagttgcg gtctgcgcga cgggctttcc
    23821 aaagccggac aattctgcat tgatatccag cttgccgccg cattccgtgg agaagtagat
    23881 aaaggcctgt tcttcagagg taaagacccg ctgcccttcg gcaatgccat ccgcaccgtc
    23941 cgcgagacga tacaatatct gctgacgggg agcgaacctg ttgcaacagt aggacgctga
    24001 tgtcaaaaca gccgatactg tatcaaaatg ccgtctgaaa attttcagac ggcatctctt
    24061 tatttgcttt cagtattatt gaaaagttta tccatcgggg tcagatccac cgcattgcct
    24121 tggctggtaa tccatttgtt ctcgacgcgc cacacgcctg ccgcgtcctc cacgcgcaca
    24181 taccagtggt tggtcggagg aagggttttg aacaccgcct catattccgc cctgccgttc
    24241 tgcgcgctgc cgacgggctt gagggcgacg gtttgatcgt ccgccttgcg ggtcgggtgc
    24301 atcagcagca ggttcaaagg ctgtttgccg tcaaactcgc cgccgacaaa cacttttgcc
    24361 gcattcatat cgggggaaat gagaacctgc accccgatat gccgtctgac agcctcttca
    24421 tcccgatgaa gctggatgtc gatatgtttg ccgtctttat aataatcgtc cgtaaccaaa
    24481 tctgtcgcgt gctgctgtgc gacaaaaaac atagcgacgc tggcgatgac gacaaaaatc
    24541 ggccccgcca tcaagatcca cggccagacg tgtttgtacc aaggtttgat tggagtgttt
    24601 tgagacacgg ttattctccg ataaaggttg catcttcttc caagacgacc ggcttgccgt
    24661 cgggcgcgcc gctttcgcgg tattggaagg taaattcgat agggtggctg cctttgtccg
    24721 cgtattccgg aatggtggac acttggacgg gaagggttac cgtttcgcgc ggggcaacct
    24781 tgataccgcc ttcgggcagc ccggtcaggg cgatttcgtc aaagcctttg acacttgcgg
    24841 taatgagctg ttctttttca cttttgttga tgatacgcag gctgtatgcg ttttccagcc
    24901 agcctttggc gttttcgcgc accagtacgc cacggtcttt caaaatatcg acctcgacca
    24961 ttttgcgcgt ggacaaaccg gctaggaagg caatgataac taacgccaac accgcgccgt
    25021 aacctgccac gcgcggtctg agcagccgtt ttttaatgtc tttttcagaa tattcgtgtt
    25081 ccagcgcgct ttcggtcgta taacggatta atccgcgcgg atagcccatt ttgtccataa
    25141 tctcatcgca cgcgtcgata caggcggcgc agccgataca ttggtattgc agaccgttgc
    25201 ggatgtcgat gccgacgggg cagacttgga cgcacatcgc acagttgatg cagtcgccca
    25261 aacccgcctc ttccttattg accgttttct tgcgcgcgcc gcgcggttcg ccgcgttccg
    25321 cgtcataaga aacaatcagc gtgtccttgt cgaacatcgc gctttggaaa cgtgcatacg
    25381 gacacatatg caggcatact ttttcgcgca taatgtgggc gaagaagaag gtcataaagc
    25441 cgtaaaacgc tgtggcaaac atcgcgccgc cgcctgctgc tccagtgaat aaatcgggaa
    25501 cgaactggcg gatagggaca aaccagcctg caaacgtgat gcccgtccac gcgcagacaa
    25561 ggaaaatcag caggtatttg gtggctttga tgcggatttt agtgaaattc cacggcgatt
    25621 tttccagttt cagccgtttg ttcctatcgc cttcgaccag gttgtcgatc cacagcataa
    25681 tttcggtgta aaccgtttgc gggcaggaat agccgcacca cagtcgcccg gcaatcgtcg
    25741 tccaccaaaa cagcccgaag gcgcaaatca tcagcagcaa ggcaaggtaa atcaaatcgc
    25801 ccacccccaa cgacaatccg aaaatgaaga aatgccgttc ggggatattg aaaacgacag
    25861 cctgcctgcc gctccagttg aaccacggaa tgacgtaaaa cacaaactgc gtcgccaata
    25921 cggcggcgat acgcagtttg gcgaaccgtc cttccgcctt tttgggatgg atgcgttcgc
    25981 cttcgggatg gatttgaatc acgctggctc gcggatcgaa tgtttttttc gctttcggcg
    26041 cggcttttgt ttgttcggac gtgccgattc cggattccgg attgccggct tggttttccg
    26101 tggtcattct gcattcctta gatttttgat tgatggtttg cccgttaccg ccgccgtttg
    26161 cttttcagac gtcatttttc ttgtttttta aggcgttgtg tttcaagttt tgagaaaatc
    26221 cgtttttccc aaaatatatt tccgctattg tacaacttta tgcgccgtcc ggatgtatgg
    26281 ggcggataca tttcccatcc gcatcaaaac gcctggattt taccttaccg cccgaacaaa
    26341 atccgaatac ggttaaaaaa aagactaaaa aaaccgacac ccccatatcg gcagaaccga
    26401 cggcgcaagc tcataaacaa acgctatcga caatccggca cacaatctat aactttttat
    26461 ttcaaaagga ataatgtcag gcttcgcccg caaatcgaaa atccttcccc gcctgtcccc
    26521 tgccgccgcc ttcccacgcg tccgcccttt tcttgaaagc ataagcgaat cgggcgataa
    26581 tcaacgcctt ccgattatcc acttatccga aacaccagca aggaaaatac aaaatgtctc
    26641 aactggcaaa cgcaatccgc ttcctctcgg ccgatgccgt tcaaaaagcc aattccggcc
    26701 accccggcgc gcctatgggt atggcggaaa tggcggaaac attgtggacg aaattcctca
    26761 atcacaatcc cgccaacccc aaattctaca accgcgaccg cttcgtcctc tccaacggcc
    26821 acgcgtctat gctgttgtac agcctgctgc acctgaccgg ctacaaccta agcattgaag
    26881 acttgaaaaa cttccgccaa ctgcacagca aaacccccgg ccatcccgaa tacggctaca
    26941 ccgacggcgt ggaaaccacg accggcccgt tggggcaagg gattgccaac gcggtgggta
    27001 tggcattggc agaaaaaatc cttgccgccg aatttaataa agacggtttg aacatcgtcg
    27061 atcattacac ctacgtcttc atgggcgacg gctgcctgat ggaaggcgta tcgcacgaag
    27121 cctgttcgct cgccggcact ttaggcttgg gcaaactgat tgttttgtat gacgataaca
    27181 atatttccat tgatggtaaa gtggacggct ggtttaccga aaacatcccg caacgctttg
    27241 aaagctacgg ctggcacgtc gttcctaacg taaacggtca tgacaccgcc gccattcaag
    27301 ccgccatcga agccgcacgt gccgaaaccg gcaaaccgtc catcatctgc tgcaaaacct
    27361 taatcggcaa aggcagtgcc aacaaagaag gcagccacaa aacccacggc gcacctttgg
    27421 gcgcggacga aatcgaagcc acgcgcaaac atttgggctg gacttacccc gcctttgaaa
    27481 tcccgcaaga aatttacgat gcgtggaatg ccaaagaaca aggcgcgaaa ctggaagccg
    27541 actggaacga actgttcgcg caatatcaag ccaaatatcc tgccgaagcc gcagaatttg
    27601 tgcgccgtat ggataaaaag ctgccggaca atttcgatga atacgttcag acggcattga
    27661 aagaagtgtg cgccaaagcc gaaaccatcg ctacccgcaa agccagccaa aacagcatcg
    27721 aaatcttggc aaaagagttg cctgaattgg tgggcggttc ggccgacctg accccgtcca
    27781 acctgaccga ctggtcaaac agcgtctccg ttacccgcga caaaggcggc aactacatcc
    27841 actacggcgt gcgcgagttc ggtatgggtg cgattatgaa cggtcttgcc ctgcacggcg
    27901 gcgtaaaacc ctttggcgcg accttcctga tgtttagcga atacgaacgc aacgccttgc
    27961 gcatggcggc actgatgaaa atcaaccctg tatttgtgtt cacccacgat tccatcggtt
    28021 tgggcgaaga cggcccgacc caccagccga ttgagcaaac cgccaccctg cgcctgattc
    28081 cgaacatgga cgtatggcgt ccgtgcgaca ccgccgaatc tctggtggct tgggcagaag
    28141 ccgtcaaagc cgccgatcac ccgtcctgcc tgattttcag ccgtcaaaac ctgaaattcc
    28201 aagcgcgcag cgaacaacaa ctggacgaca tcaaacgcgg cggctacatc atcagcgaag
    28261 cccaaggcaa cgcccaagcc gtcatcattg ccaccggctc agaagtcgag ctggctttgg
    28321 aagcgcaaaa agccctcgcc gcgcaaaaca tcgccgtgcg cgtcgtttcc atgccgtcca
    28381 ccaacgtatt cgaccgccaa gacgccgcct atcaagccgc cgtcctgccc gaaagcctgc
    28441 cgcgcatcgc cgtagaagcc ggccacgccg acggctggta caaatatgtc ggactgaacg
    28501 gcgcagtcgt cggcatcaac cgcttcggcg aatccgcccc tgccgactta ctcttcaaag
    28561 cattcggctt taccgtggac aatgtggtcg atacagtgaa atcagtgctg taaccccact
    28621 cctaaacaaa tgccgtctga acgcttttca gacggcattt ttttgccctc ccgccttgat
    28681 gcttcctcat tccaccaatc tccattataa tatttgcgaa tcactcttat tcacatttca
    28741 aaaggagaaa cgtatgaaca ctcgcaccga acacgacacc atgggcaatg tcgaagtccc
    28801 gtccgaagcc tattggggcg cgcagaccca gcgcagccgc aacaatttca aaatcggcgg
    28861 cgaaaccctg ccgcagccgc tgatttatgc tttggcgttg gtgaaaaaag ccgctgccgc
    28921 gaccaatgtt tccctcggca ggattaagcc ggaacaggcg gatttgatta cgcaggcggc
    28981 ggatgatgtg ttgaacggca agctcgacgg acagtttcct ttagtggttt ggcagaccgg
    29041 ctccggcacg cagtccaata tgaacatgaa cgaagtgctg gcgaaccgcg ccaacgaaat
    29101 cgccggtacg ggtttggcgg cgtatcagcc cgtccatccc aacgaccatg tgaaccacgc
    29161 gcaatcgacc aacgacgcat tcccgaccgc catccacgtt gccgccgcga ttgaaatcaa
    29221 ccgccacctc atccccgccg taaaagccct gcgcgacacc ttggacaaaa aagcccaagc
    29281 cttcgcccct atcgtcaaaa tcggccgcac ccacttgcag gacgcgacgc cgctgacttt
    29341 aggccaagaa ttttccggct acgtttccca gctcgaccac ggcttaggcc gtctgaacga
    29401 cgcgcttaaa gacttgtatg aacttgcttt gggcggtacg gcggtcggca cgggtttgaa
    29461 cagccatccc gaatacgccg aaaaagccgc cgccaaactc gccgaattgt ccagcttgcc
    29521 gtttgtcagc gcgccgaaca aatttgaagc cttgggcgga cgcgatgccg ccgttgccgc
    29581 ttcgggcgca ttgaaaacgc tggcggcaag cctgaataaa atcgccaacg acatccgctg
    29641 gctggcaagc ggcccgcgct gcggtttggg cgaaatcaaa atccccgaaa acgagccggg
    29701 ttcgtccatc atgccgggca aagtcaaccc gacccaatgc gaagcgatga ccatggtgtg
    29761 ctgccaagtg ttcggcaacg acgttaccat cggcatggcg ggcgcgtcgg gcaatttcga
    29821 gctgaacgtc tatatgcccg ttatcgccta caacctcttg caatccatcc gcctcttggg
    29881 cgacgcgtgc aacagcttca acgaacactg cgccatcggc atcgaacccg taccggaaaa
    29941 aatcgactat ttcctgcacc attccctgat gctcgttacc gcgttaaacc gtaaaatcgg
    30001 ttacgaaaac gccgccaaag tcgccaaaac cgcctacaaa aacaacaaat cgttgcgcga
    30061 aaccgccgtt gagttgggct tgctgacggg cgaagaattt gacgaactgg tcgttcctgc
    30121 cgatatggtt catccgcgct aatctttccc tcaaataaaa tgccgtctga aacctcgttc
    30181 ggacggcatt ttccgttgcc tgcgaactag cggcgtttga acagcctgtc ccccaccgcc
    30241 gccgtaaccg cacccccgac cacgaccagt gcgccggcat aacccaaacc gttcatatcc
    30301 ggcgcggcaa aagtatcagg catcacataa tgcccgagca aagaaaatat tacggtaaac
    30361 acggggagca aggttgttac cgcgctgact ttggaagcct cccaatgttt caacgcctcg
    30421 ccgaacgagc cgtaaccgat taacgtattc aagcagcaat acgcaaaaca aacccacgcc
    30481 aacgtaccgt ccaaacttcc gatgtgtgcc ggtttggcaa acggcaggaa cacggcggca
    30541 cttgccgcat aaatcaacag cagaatctgt tgcggtccga attgcgccga cagcagcttt
    30601 tgcgccacgg cataacacac ccatgccata ctgcctgccg cacacagcaa cacgcccttc
    30661 gcatacgcgc ccaaacccga caactcgccg aatttatcgt taaaaaacat aagcaaaccg
    30721 gcaagcagca aaaccaagcc gattttctga gcggcagtca tccggtcttt aaacaccaac
    30781 acgccgacaa caatcatcgt aaacggcgaa atctgccaca aaacctgcgt cgtggtcggc
    30841 gaaatataat gcagcccttg ggcaatcagc acaaagtttg ccgaaatgcc cgccacgccg
    30901 agcagcagca gcctgaatga gcaccaagaa aaatcccgcc gcttcggcag ccgcccgccc
    30961 aatgccagca aaacaaacaa taccgccgcc gccacggtaa aacgcaccca caccagcgtc
    31021 ggcgcatcga caaacttcaa tacctgccgc acggcaatcg gcagcgttcc ccacgtcatc
    31081 gccgccaaaa gtgccaacgc gaagcctagg tgcggccttt ggttttccat cctgattttc
    31141 ctatttttaa acaaccgtat tgccggacga tgccggtttg ccgcatcggg caataatggt
    31201 tcaagcgttt ggcgtttaat tccaaccctt tgatttcaaa caaaccggct gaagctcggc
    31261 tattgcttcg cgctatttga aaacaccgcc tgaattttaa a
//
LOCUS       DAXHTY010000014        31048 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXHTY010000014 DAXHTY010000000
VERSION     DAXHTY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.014"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(78..680)
                     /locus_tag="ACFNMK_000539"
     CDS             complement(78..680)
                     /locus_tag="ACFNMK_000539"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6363864.1"
                     /translation="MPTLSQGLPNNYIYRENGVEFGLVQIRYPEHIELLHRWMNMPHV
                     IPQWQLNKSLSELSVYFEKMLADDHQRLYLVSINGEWVGYAEVYEGARDRLSGYYTAD
                     KNDLGWHLLLAEAQAVGKGYLRAIMRLLSFFIFEHSPAKKIVGEPDASVKPYEVVAQE
                     LNYMPQYQIVMPEKTAMLYFCDKEGFYCKFAHYLNASIKE"
     gene            complement(865..1113)
                     /locus_tag="ACFNMK_000540"
     CDS             complement(865..1113)
                     /locus_tag="ACFNMK_000540"
                     /inference="COORDINATES: protein motif:HMM:NF018031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family C-terminal-domain containing
                     protein"
                     /protein_id="HFC6363865.1"
                     /translation="MSEFEFWNELSKIIDEFHHQHPELNERIALFDLKRPKFEKVCLN
                     RVRFFTRGYQDNANRPEPVVCEPICNPISPQFLRCVEH"
     gene            complement(1293..1604)
                     /locus_tag="ACFNMK_000541"
     CDS             complement(1293..1604)
                     /locus_tag="ACFNMK_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6363866.1"
                     /translation="MEYLTTTIPTHFEIERTRLADVILTKLSDDKAVKLAKQMLDEHE
                     YIESLLVNTDPSVDDVKELANALYDHIRFEERELFPIAETVLSDDELFAIYEASDENV
                     K"
     gene            complement(2133..2825)
                     /locus_tag="ACFNMK_000542"
     CDS             complement(2133..2825)
                     /locus_tag="ACFNMK_000542"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC6363867.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHSLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            complement(2893..4869)
                     /locus_tag="ACFNMK_000543"
     CDS             complement(2893..4869)
                     /locus_tag="ACFNMK_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6363868.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIAAPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPNTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIVPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            5047..5712
                     /locus_tag="ACFNMK_000544"
     CDS             5047..5712
                     /locus_tag="ACFNMK_000544"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6363869.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGIPSQILSEVHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SIGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPEWDGYLGGKALAAGAAR
                     VAD"
     gene            5764..6591
                     /gene="mutM"
                     /locus_tag="ACFNMK_000545"
     CDS             5764..6591
                     /gene="mutM"
                     /locus_tag="ACFNMK_000545"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC6363870.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYERLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPRCGGLVVKETLGQRGTFYCPNCQK"
     gene            6679..7446
                     /locus_tag="ACFNMK_000546"
     CDS             6679..7446
                     /locus_tag="ACFNMK_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC6363871.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(7757..8068)
                     /locus_tag="ACFNMK_000547"
     CDS             complement(7757..8068)
                     /locus_tag="ACFNMK_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC6363872.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYKEQKTQCQAAYEAFDDFDGSRFR
                     R"
     gene            8597..10876
                     /gene="nrdA"
                     /locus_tag="ACFNMK_000548"
     CDS             8597..10876
                     /gene="nrdA"
                     /locus_tag="ACFNMK_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC6363873.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            10934..11947
                     /gene="dcm"
                     /locus_tag="ACFNMK_000549"
     CDS             10934..11947
                     /gene="dcm"
                     /locus_tag="ACFNMK_000549"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFC6363874.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            11940..13127
                     /locus_tag="ACFNMK_000550"
     CDS             11940..13127
                     /locus_tag="ACFNMK_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HFC6363875.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            13188..14321
                     /gene="nrdB"
                     /locus_tag="ACFNMK_000551"
     CDS             13188..14321
                     /gene="nrdB"
                     /locus_tag="ACFNMK_000551"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC6363876.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            14546..14836
                     /gene="yfaE"
                     /locus_tag="ACFNMK_000552"
     CDS             14546..14836
                     /gene="yfaE"
                     /locus_tag="ACFNMK_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC6363877.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(14877..15155)
                     /gene="ftsB"
                     /locus_tag="ACFNMK_000553"
     CDS             complement(14877..15155)
                     /gene="ftsB"
                     /locus_tag="ACFNMK_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC6363878.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(15169..16455)
                     /gene="eno"
                     /locus_tag="ACFNMK_000554"
     CDS             complement(15169..16455)
                     /gene="eno"
                     /locus_tag="ACFNMK_000554"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC6363879.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYLGKGVLKAVDHVNNQIAQALIGIDASEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDTGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEVAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            complement(16501..16941)
                     /locus_tag="ACFNMK_000555"
     CDS             complement(16501..16941)
                     /locus_tag="ACFNMK_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC6363880.1"
                     /translation="MMQTFRKISRYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GILFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKEAV"
     gene            complement(16956..17798)
                     /gene="kdsA"
                     /locus_tag="ACFNMK_000556"
     CDS             complement(16956..17798)
                     /gene="kdsA"
                     /locus_tag="ACFNMK_000556"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC6363881.1"
                     /translation="MDIKINDITIGNNSPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LSIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPYQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASSGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            complement(17820..18203)
                     /gene="panD"
                     /locus_tag="ACFNMK_000557"
     CDS             complement(17820..18203)
                     /gene="panD"
                     /locus_tag="ACFNMK_000557"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC6363882.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            complement(18352..22050)
                     /gene="mfd"
                     /locus_tag="ACFNMK_000558"
     CDS             complement(18352..22050)
                     /gene="mfd"
                     /locus_tag="ACFNMK_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC6363883.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHTLPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIR
                     DPVVHEEHGIGRYMGLVTIDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     NIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINELDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIE
                     SHHLRLMAKELGINAIDAAGEAVTVTFGKNNNIDPTEIILLIQNDKKYRLAGADKLRF
                     TAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            22235..23662
                     /locus_tag="ACFNMK_000559"
     CDS             22235..23662
                     /locus_tag="ACFNMK_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6363884.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKRHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDTAAFLLNDIRKDILDCRYCG"
     gene            23811..24902
                     /gene="mltB"
                     /locus_tag="ACFNMK_000560"
     CDS             23811..24902
                     /gene="mltB"
                     /locus_tag="ACFNMK_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC6363885.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRVNEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAVIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(25025..27031)
                     /locus_tag="ACFNMK_000561"
     CDS             complement(25025..27031)
                     /locus_tag="ACFNMK_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC6363886.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQVHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNS
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGVIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            27320..29347
                     /locus_tag="ACFNMK_000562"
     CDS             27320..29347
                     /locus_tag="ACFNMK_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC6363887.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPNQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLVTFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(29439..30992)
                     /locus_tag="ACFNMK_000563"
     CDS             complement(29439..30992)
                     /locus_tag="ACFNMK_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC6363888.1"
                     /translation="MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTGQSRPDGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
BASE COUNT         7302 a         7136 c         8607 g         8003 t
ORIGIN      
        1 cgcctgaaaa atcgagtaaa aatacaggta aggagctatt tgggttctga atagcattta
       61 gaaaaatagg cataacatca ctctttaatg ctggcattta aataatgggc aaacttacaa
      121 taaaaccctt ctttgtcaca aaaatataac attgcagttt tttcaggcat tacaatttga
      181 tattggggca tatagtttaa ttcttgagcg actacctcat agggttttac acttgcatca
      241 ggttcaccca caattttttt agcaggtgaa tgttcaaaaa taaaaaagct tagtagacgc
      301 atgatggcac gcaagtaccc tttgccaaca gcctgtgcct ctgctagtaa caaatgccaa
      361 cccaaatcat ttttgtcagc tgtataatac cctgatagtc tatcccttgc cccttcataa
      421 acttcggcgt aaccaaccca ttcgccatta atactaacca aatacaaacg ctggtgatca
      481 tcagccaaca ttttttcaaa ataaacagac agctcagaca atgatttatt gagttgccat
      541 tgagggataa catggggcat attcatccaa cgatgcaaca attcaatatg ttcagggtag
      601 cgtatttgca ccaacccaaa ttcaacccca ttttctctgt aaatatagtt gtttggcaac
      661 ccctgagata aagttggcaa tcgattgatt ttactgcatt gatcttgatt aaaagaatcg
      721 gtatagattt ctagcatttc tattctctca ttagggtgtg ttgaatattt ggtatttgca
      781 atgaaaaatg agaaaaatcg cacgtttttc atgcaaaaac taataaaagc gttaaaattt
      841 tcatcatttt tacaaataat gttatcaatg ttcaacacac cttagaaact gtggtgatat
      901 tggattgcaa attggttcgc aaaccacggg ttctggacgg ttggcgttat cttgataacc
      961 tcttgtaaaa aagcgaacac gatttaggca caccttttca aattttggtc tttttaaatc
     1021 aaataaagca atcctctcat ttagctctgg atgttgatgg tgaaattcat caataatctt
     1081 tgataactca ttccaaaatt caaattcaga tatattataa tattaaagct actttgtggc
     1141 ggacagcgtg gagcatagca ccgataaacc tgtgtttaat cagattgtgg gattaaaaga
     1201 tgggtttaaa taacttttag caattttaaa attaaacgaa agccaaacgt ggtgtttggc
     1261 tttttaattc cattgcccag cttttaactc gttcatttta cattctcatc actcgcctca
     1321 taaatggcaa aaagctcatc atcgcttagc accgtctcag caatgggaaa aagctctcgc
     1381 tcttcaaagc ggatatggtc atacagagca tttgcaagct ccttgacatc gtcgacgctt
     1441 gggtcggtat tgacaagtag gctctcaatg tattcatgct catcaagcat ttgctttgcc
     1501 aatttaaccg ctttgtcatc ggataatttt gtcaaaataa catcggcaag gcgtgttcgc
     1561 tcaatctcaa aatgcgtggg aatggtagtg gtcagatact ccacttaggt taatcggctg
     1621 gatcaggttt gacgattggt gttggaagac atattgcaac agtgtttgat taagatgaga
     1681 tggaatttct acacgcgttt tttaacagga actgactgat tgttttttaa gtgtgtagtt
     1741 attttcacta atagagttat attttataat ggatatttct gatttgcata aatcaacaag
     1801 gattgaatca gattttatag atagatgaat cgtttatttt tctacactgg gacggtgaaa
     1861 taaaatttca tatgtcatat gatcgggaat gctgcgtcag taacatatgt agtatgtgta
     1921 gtaaacgaag ataaaaaaac catctataca aggggagaag tatagatggc aaaacacatt
     1981 acggggaaaa cgtcttactc ataagcctgc ttgaacaggc gttactcaga caaatggatt
     2041 atatcgtaaa ttgatttttt gcgttaaatt gggttaaacc atatatttaa tgagtatgtg
     2101 gatgaatata aatggaattc ttggatgtta tttcagaatt ttggacattc gataaatttc
     2161 cattccccgg gtttgaggtt ttctgtttcc agatgtgcga atcgccggcg atgaaggtgt
     2221 tgcacgcggt tgccggcggc ggcgatcatg cgtttgactt ggtggtattt tccttcggta
     2281 atggtcagca gcagggtggt cgggtttttc aaaacggcat cggcggcaca aacggtttcg
     2341 ttttcgtcgt ggagcagcac gccgtttttc aaggtttcgc agagcgtttc tcctgtgggg
     2401 tgtttgagcg ttacttcgta cagcttggga atttttctgc tcggcgaagt caggctgtgg
     2461 ttcagtttgc cgtcgttggt aatcagcaat acgccggtcg tatctgcatc cagcctgccg
     2521 accgcctgca tatcgatgtt ccgcatattg tcggggaaca ggctgaatac gctgcggtag
     2581 tgcttgggtt tgtgcgaagt ttcgtaatct tcaggcttgt tgagcatgat gtagaaatag
     2641 ggttcgggaa cgacggttac tgcttccccg tcaatatcca acgtttcgac ggatgaggaa
     2701 tcgatgtctg catcggtgtc gtccatgcag gttccgttga tgaaaacata accgccggca
     2761 atcagccatt ggcactgctt gcggcttcct atgccttgat attgcaggta tttgataagt
     2821 ttcatgatgg tatgggaaat gtgggatgaa aaagacagga ctgagaaagt cctgtcatgt
     2881 gccgtatccg aatcagcttc caatcagttt gaccctctgt cccggattga tggtgttcag
     2941 gttgggattg agccggcgga tgtcgtcgat atggatattg aagcgcgcgg cgatactttt
     3001 gaaggtgtcg cctttgcgcg cggtgtagga tactttttca atacgggttt gggcaggggc
     3061 tgcctgcgcc aggcggagta cctgtccttt ttggatggtg ttgcctttga tgttgttggc
     3121 gacaatcagg tcggctacac tgacgttgta gcgtttggca atgttgaaca gggtgtcgcc
     3181 ttctacaacg cggtggatgc tggcatggag cggggagatt tcggcttcgc cgtcggcagc
     3241 tttggctacg cgcgtttcca aacgtacccg gcgtttgccg tcggcggcaa cggtatgttg
     3301 cggagatgag gaaatgttgt gttcggcaac tgtgtcaggc gtgccgatga cggcagagat
     3361 ggtttcttca gcctgtcggc gcaggttgtt tcgggcaaca agctgcatga gttcgtctgc
     3421 cgcctgcatg tcgttttggg ggacgatctg cgcgagtgtt gcggtttggg tttcagacgg
     3481 catggcggtc tgtttttcgg tttgaggttg cgctgcggct gtcgcaaggg caggttcggc
     3541 aatacggaca aggggatcgg gttccgtacg gacggttttc tgcggcaaag gtgcgacggt
     3601 aatgtccgct gtctgtgcgg cggcgggtcg gattcgggca atgccgacgt tcaccgtgcc
     3661 tgccggcata ttggaacggt aggtgttggg cgtattgtcg atgtcgatgg aaacgacgga
     3721 ttccgatgcc gtctgaaggg ttttgccgtt cttggcgaca aggatgctgc gtcctgcgtt
     3781 gacaaggttg ccgttcaggt tgttgaggcg tttgatgtcg gcaatgctca tgccggttgc
     3841 cgtcgagatg tcggacaagc tggttttggc ggcaggcgta tagacttccc atgaaaacag
     3901 gctgtcgggt gcggcgttga ggtagttgct ttggaaggtt tgtacggacg cgacaggaag
     3961 cagcagtttg cgtttgcttt tggggatgaa cgcggggacg ttgaatgcgg ggtttagggc
     4021 gagcagctcg ctttgcgtga tgccggcaag ccgggcgatg gcttcgttgt cgagcggacg
     4081 gtccggttcg actgcctgaa aatacggttt gttgtctatg tcgctgatat tcatgccgaa
     4141 agattggggg gcggcaatga tgttgcgcac ggcgagcagc ttggggacat agttgcgcgt
     4201 ttcgttgggc atacgcaggt tttcgtaggt cggttcgagc ccttgggcgc gggcgcggtt
     4261 gatggcgcgt ccgacgttgc cttcacccca gttgtaggcg gcaaaggcaa gcggccagtc
     4321 gccgaacagt ccatagaggt attgcagata gttgagtgcg gcatcggtgg cggcgtaaac
     4381 gtcgtgcctg ccgtcgtaaa ccggtgtttt ttccaggccg taatgcctgc cggtagcggg
     4441 cataaactgc cataatcctg atgcgccgac gtgtgatttg gctttggtga cgaacgcgct
     4501 ttcgatgaag ggaagcaggg cggcttcggc gggcatattg cgttttttga cttcgttggc
     4561 gatatggtac atatagggtc tactccggtt gatgaccctg ttgaaatagc tgtggcttgc
     4621 gatgaatttg ctttcgtggc ggcgtaccag ttcgggattg acttcgccca tccggaagcc
     4681 ttggcgcagc tcgctccaca ggctgccgga ttggaaatat tgttttgtcg gtggcaggtc
     4741 gagtattgaa gagtttaagc gcataatcgc caaaccgatt tggtgtgatg aggtgttttg
     4801 ggcgtatagg aaacccggac aaacggacag acctgacgcg gtcagggcga tggttttgag
     4861 tttggacatg atttatatcg gcggaataaa catattttat cgtccgatgg tactgtttga
     4921 ctgaaaaagc gtcaagtttt acaggaactt tatgcggatt cggaaatggg cggccgatgt
     4981 gttttttgac agaaatcctg tttggaatag aatatcggca ggcaatctgt ttgaaacgga
     5041 cgcaatatgg atgcatggtt tgaagatacg gcgatggggc ggtatgttgc aaaattggaa
     5101 caggatttct tcgggcggta tctagattca taccgttttt cgggaatgtg tgcggttcag
     5161 gtgggcggtc cgtggctgag cctgtctgaa gatgttgtct gtgtgccgcg agatatgtcg
     5221 atgtcggcgg agaatatggc tttggcggat gtttctgcgg atatgctgct ttttccgcat
     5281 acgcttgaag gcggtatccc ttcgcaaatc ctgtccgagg tgcaccggat attgaaaccg
     5341 tgcggacgct tgatgctgac gggtttcaat ccgtattcgc tctggggatt cagccgttgg
     5401 tttgacggcg aacgcctgcc ggaaaaacgg ttttgtctgc cgctgcccga gttgaaaagg
     5461 cagcttgcgg atgtcggttt cgatattgaa tttgggaaat ttatggtgta tctgccgccc
     5521 gtttcgtcga tcgggcaaat acgcttttgg cggtttatgg aaaaggcggg cgaccgttgg
     5581 tggccgcaat gtgccgcagt gtacggtttg gttttggtca aaagggcggc gggcgtaacg
     5641 cccctgcccg agtgggacgg gtatttgggc ggcaaagccc ttgcggcggg tgcggcaagg
     5701 gttgcggatt agggacagcc gtctttcaga cggcatctgt cagaagaaat gtcgggaggg
     5761 gttatgcctg aattgccgga agtggaaacg acgttgcgcg gcatcgcgcc gcatattgaa
     5821 gggaaaacgg tggaagccgt ggtattgcgc caattgaagc tgcgctggca gatcaatccc
     5881 gatttggggg agattttgtc cggccggcag gttttgtcct gcggcaggcg ggcgaagtat
     5941 ctgattgtcc gtttccaaac ggggattttg ctgattcact tggggatgtc gggcagcttg
     6001 cggattttta cgccgtcgga cggacgtatc ggcaggccgg acagacacga tcacgtcgat
     6061 attgtgtttt cagacggcac ggtcatgcgt taccgcgatc cgagaaagtt cggcgcgata
     6121 ctttggtatg agggaattga agagcatcat ccgctgctgg aaaaactggg gccggaacct
     6181 ctgtcggaag cattttgtgc ggattatctg tacgaaaggc tgaaggcgca gaaacgggcg
     6241 gtcaaacttg ccctgatgga caatgcggtc gtggtcggtg tgggcaatat ttatgccaat
     6301 gagagcctgt tcagagcggg catttcgccc caccgtcctg ccaaccgtct gaaaaagaaa
     6361 gagtgcgcgc ttttggttga aaccgtcaaa gcggtgttgc agcgcgccat tgaaacgggc
     6421 ggcagtacct tgagggattt tgtggacagc gacggcaaaa gcggctactt ccaacaggaa
     6481 tatacggtgt acgggcggca caatcagccg tgcccccggt gcggcggttt ggttgtgaaa
     6541 gaaactttgg ggcagcgcgg cacgttttat tgcccgaact gtcagaaata gggctgaaaa
     6601 cggtttcaga cggcatttta tcggtatgcc gtccgaacgt ttcaacaaca aacaccgatt
     6661 atcgggaaag aattgctcat gtcttcaaat aaagcttcat tttttacacg tctgcgccgc
     6721 ttgtgccgtt tggcggtctg gctgttcaaa accgggaaaa acctgcgcgg tattgacggc
     6781 ggttgccccg agtcgcgcaa tcgggcggta atcgagttgg gcaggggggt tttggcggct
     6841 ttggatatcg gattggaggt gggcagaccc gcacccgaac atccgaacgg tgtcttggtt
     6901 gccgccaacc acgtgtcctg gctggatatt ttcgcgatga gcgcggttta tccgagcagc
     6961 tttatcgcca agcaggaaat caaaagctgg ccggtattgg gcaagatggg gcagaacgcg
     7021 ggaacggtgt tcatcaaccg caattcgcgg cgcgacatcg aaccgattaa ccgcgccgtc
     7081 tgcgaaacct tgcaacgcgg tcaaaacgtc agttttttcc ccgaagcgcg gacttcctcc
     7141 ggattggggc ttttgccgtt caaagccgcg ctgttccaat ccgccatcga tgcgggggca
     7201 aaggttttgg cggtcgcgct gcgttattat gacgaaacgg gaaaaaggac ggctcgtccc
     7261 tcatatgccg atgtcggttt gccgacctgc ctgtggcgca tcgtgtctat gaaaaaattg
     7321 acgataaaag tcgatttcgt ttgcgtggcg gatgcggcgg aaagcgaaga ccgttatgct
     7381 ttaaaagata aaatcgaaga aagcatccgt gccgttgtcg ccgacgatgc ggatatcgct
     7441 gtctgaaacc ggttgtcgga atgtggcagt atgattcgct tttgtggatg tatagtggat
     7501 taacaaaaac cagtacagcg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg
     7561 ctgaagcacc aagtgaatcg gtttcgtact atctgtactg tctgcggctt cgtcgccttg
     7621 tcctgatttt tgttaatcca ctatattaag gctgcggcaa cgggaggacc tcaaacgaaa
     7681 tggcgtaagg atagtttgtt gtattcataa aatatgccgt ctgaacctgt gttcagacgg
     7741 catattttat atgggattaa cggcggaacc tgctgccgtc gaaatcatcg aaagcctcat
     7801 aagccgcctg gcattgggtt ttctgttcct tataaggcag ggagcggaat gctttgcgcg
     7861 cctttttgtt ttcgcgcaat tcctgcacgt tgccttgcga tcggtaccac gcggcacggc
     7921 gttccaaata tttcctgcat tcggggtgca gcatctgctt gtttgccttg cggatatgtt
     7981 tggtatggcg gtgggcgggt tctgacgtac cggcagcggc agttgtcgat accagtccca
     8041 atattgcaga caacagtatg gcaagcatct ttttgacaga tatgacgatc ctttcagttt
     8101 gtcattcctt ccagtatagg atggaaggat ggaacgggca aattgtcggc agtttcagac
     8161 tcattcagcc gacaaaactt gccgtttgat atgcggattc aaataacaca ttaatctcaa
     8221 taataaattt caatcggtaa gaaaatggaa tttgtcggcg gcggcgcggc aaaatcatac
     8281 tttgcaaaat ttaacaattc gcaggggcag aaaaccggaa gctttccttt ttcgtcggaa
     8341 aatccttatt tcaccgcctt gtagccggag ccggtcaaaa ggcaaaaaat ttacccgttt
     8401 tttatcggta aagaattatc agataaaaca aatattatag gaaaaatacg acaggcgggt
     8461 tttatcgcgc attgcctgaa actgaaaaat gcaaccgttg tcaagactgg agaaaatgcc
     8521 aaaaatccac tatattgtct gccttaattt atttgaaaag actgtgtctt gaatatcaag
     8581 agtggaagag gaagcgatga atacaccgac tgatttgaaa gtaaccaaac gagacggaag
     8641 attagaagcc attgatttgg ataagattca ccgtgtcgtc acttgggcgg cggacggatt
     8701 ggaaaatgtt tccgtatctc aggtcgagtt gaaatcgcac atccagttct acaacggcat
     8761 ccgcaccgac gacatccacg aaaccatcat caaagccgct gccgatttaa tttcggaaga
     8821 taccccggac taccaatacc ttgccgcgcg tttggcgatt ttccatctgc gtaaaatagc
     8881 ctacggcgag tacgagccgc cgcaccttta cgaccacgtt aaaaaactta ccgatgccgc
     8941 aaaatacgac aggcatatcc ttgaggatta cagccgcgaa gaatttgacg agctgaacgc
     9001 ctatatcgac cacgaacgcg atatgtcctt ttcctatgcc gctgtcaaac agctcgaagg
     9061 caaatatctg gtacagaacc gcgttacccg ccagatttat gaaacaccgc agttcttata
     9121 tgttctggtg gcgatgtgtc ttttcagcaa atatcccaaa gaaacacgcc tcgattacgt
     9181 caaacggttt tacgatgccg tttccacatt taaagtatcg ctgccgaccc cgattatgag
     9241 cggcgtgcgt acgcctacgc gccagttctc aagctgtgtg ctgattgaat gcgacgatag
     9301 tttggattcc atcaatgcca ctaccagcgc gattgtgaaa tacgtttccc agcgtgcggg
     9361 catcggcatc aatgccggac gtatccgcgg tttggacagc gaaatccggg gcggcgaagc
     9421 gcggcatacc ggctgcatcc cgtttttcaa aatgtttcag gcggcggtca aatcctgttc
     9481 gcaaggcggc gtgcgcggcg gcgcggcaac cttgttctac cccttgtggc atatcgaagc
     9541 cgaaagcctg ctggtgttga aaaacaaccg gggcgtggaa gacaaccgta tccgccagct
     9601 tgattacggt gtgcaaatca accgcctact gtacacccgc ctgattaagg gcggcaacat
     9661 tacgctgttt tcgcccaacg aggttccggg attgtacgaa gcgttttttg ccgaccaaga
     9721 cgaatttgag cggctctata cgaaatacga gcaagaccct gatatccgca agcgcatcat
     9781 tccggctgcc gacctgtttt ccacgctgat gcaggagcgt gccggaaccg ggcgcatcta
     9841 cattcaaaac gtcgatcact gcaatacgca cagcccgttc gatccgcgcg tcgcgcctgt
     9901 tcatcagtcc aacttgtgta tggaaatcgc cctgccgacc aaaccgctgg acaatatcaa
     9961 cgatccgaac ggcgaaatcg ccctgtgtac cctgtccgcc ttcaacttgg gcgcattaaa
    10021 cagcctggac gaattggaag ggcttgccga tttgactgtg cgcgcactcg atgcactttt
    10081 agattatcag ggatatccgg tagaagccgc gcgtacctct actatgggtc gccgttcgct
    10141 cggcatcggc gtcatcaact atgcttatta tctggcgaaa aacggtgtcc gctacagcga
    10201 cggctccgcg ctcggtctga cccaccgtac ctttgaagcc atacagtatt acctgctcaa
    10261 agcatcggca aaccttgcca aagaatacgg tgcgtgtacg ctctttaacc aaaccgttta
    10321 ttcgcaaggc aaactgccca tcgacaccta caaaaaagat ttggatgccg tctgcggcga
    10381 gcctttgcat tacgattggg aaagcctgcg tgccgaaatc gtcaaatacg gcctgcgcaa
    10441 ctctactctg accgcgctca tgccgtctga aaccagctct caaatcgcca acgccaccaa
    10501 cggcatcgag ccgccgcgcg gattggtaac ggtcaaagca tcgaaagacg gcattctgaa
    10561 gcaagtcgtg ccggagtttg aaaccctgaa aaatgcctat gaaaccctgt ggcagcttcc
    10621 cggcaacgaa ggctacctga aacttgtcgg cgtgatgcaa aaattcgtcg atcaatcgat
    10681 ttccgccaat accgcctacg acccgggcaa attcgaaggc ggcaaagttt ctatgaaaca
    10741 aatgctcaaa gacctgctga ccgcctacaa atacggcgtc aaaaccctgt actaccataa
    10801 cacccgcgac ggcgcggacg atacgcagac cgatattcag gatgacggct gcgcgggcgg
    10861 ggcttgtaag atttgatgaa agggggagtt ttcagatggc ctttagatta ataaatcatc
    10921 tgaaatataa aatatgaaaa ataaaataca aaatcaatta gataatatag ttaccttaaa
    10981 taatgagaaa tttactttta ttgatttatt tgcgggaata ggtggttttc gcattgcaat
    11041 ggagaatgtt ggtgggcgat gtgtattttc tagtgaatgg gatgataaag cccgtcaaac
    11101 ctaccaagta aattttaatg atattcctta tggagatatt acattaaagg aaaccaaagc
    11161 agctattcca agtaagtttg atgtattaac agcaggattc ccgtgtcagc cattctctat
    11221 agctggtgtt tcaaagaaaa aaagcctagg acgagaaaca ggcttcttag ataaggcgca
    11281 gggaactcta ttttttgatg ttgctgaaat tattggaaaa catcgaccta aaatttttct
    11341 tttagagaat gtgaaaaacc ttgtttcgca tgacaaagga aatacattta aagtaattaa
    11401 agggacttta gaagagcttg actatcagat attttatcaa gttatgaatg caaaatatta
    11461 tgttcctcaa aatagggagc gtatttttat tgtaggtttt gatagacaat attttaataa
    11521 ggaaataaat ttcaattttc cttccccacc agaatcacaa ccaaaattaa agcaaatttt
    11581 ggaagatgat gtagataatt cttttactct ttctgataac ttatggcttt accttcaaaa
    11641 ttacgctaaa aaacataagg caaagggtaa tggatttggt tttggattag ttgatttaga
    11701 tggaatatca cgaactctat ctgcacgata ttacaaagat ggttccgaaa tactcatccc
    11761 tcagaaagga aaaaatccta ggaagctgac acctagggag tgctcgcgtt taatgggatt
    11821 tcctaaagat tttgttattg atgcagtatc caagacagct gcatacaagc agtttggcaa
    11881 ttcaattgct gtaccgttgg ttcaagctat tgctaaacaa attataaatg agttaaaaaa
    11941 tgaatgactt ggcagctcag acaatacaag tagtaaataa atccgatgac ttagtagctt
    12001 cagcaataca gacagcaaat aaatcaattg cagtatactg tcgttatatt cgtcccaatg
    12061 atgttggtac tactggtagt caccaatcag gattttatat tcaaaaacat ttttcagacg
    12121 acctctttga tgtagtttgc caaaagggaa caaataaaac tatttcaatc aaaattaatt
    12181 ggcaagatgg aagcgtcact aatagcaatt ttaaatatta cggccaaggc acgagaaatg
    12241 aggcgagaat tacgggtttt ggcaagaatt ttgaattttt aagtgataaa tatagtggtt
    12301 ccttattggt attgtgcaga gcttgttata aagatttatt attccatgct tttgttttat
    12361 cttcagatga agatattgaa atatttatcg cagaaactaa cattttacca ggaagtttat
    12421 atttacctaa aaaacaagaa gtagaggata acctaacaaa attattttca ctatttccta
    12481 acttccccaa aactgaagaa atggcagttt tggcaaggga aaatattgca ttaaataaaa
    12541 ctaacgttag taatgtgtta aaacaatggg tttcaaagga atatgaatta ttttccattt
    12601 ttgaaaaacg tgaatttgaa attattaaat caaaaattac tgatttggat agcttcatta
    12661 attttgcaca ttcttttaca aatcgcagaa aagcaagagc aggaaaatct ttagagttac
    12721 atttatcaag aatatttgac gaattttcct taaaatttga gacgcaagcg aagacagaag
    12781 ggaaaaagaa accagatttt ttatttcctg gaagtgaaga gtaccatgcg atggatgaat
    12841 cagggaattt catatttcaa actgaaaagc tgacaatgct aggttcaaag actacttgta
    12901 aggatcgctg gcgccaagtt ttaaatgaag cagatcgaat tcctcataag catttattta
    12961 ctctacaaga aggtatatct gatactcaaa ttcaggaaat gagtgatgag aatttaacct
    13021 tagttgtccc taaagaatcc gtaaaaacat ttggaacttt tgggaaaacc catgttttaa
    13081 ctttagaaaa ttttattaaa tatataaagt ctcagcaggt tagttaaaac ttacagatca
    13141 tttttaatta atatagagaa tagattatgt cctgcgaaca cttaactatg tcatacagca
    13201 cctttcccaa aaccaaaaac gacgcgctga atgagccgat gtttttcggt cagccggtta
    13261 atgtcgcccg ttatgaccag cagaaatacg aggtatttga aaaactgatt gaaaaacaat
    13321 tgtcgttttt ttggcggccg gaagaaatcg acgtgtcgcg cgaccgtatc gactacgcca
    13381 acctgccaga acacgaaaaa catattttca tcagcaatct gaaataccaa acactgctcg
    13441 attccatcca agggcgcagt ccgaatgttg ctttgctgcc tttggtttcg attcccgagt
    13501 tggaaacgtg gattgaaacg tggagcttca gcgaaaccat ccactcgcgc agctataccc
    13561 acatcatccg caatattgtg aatgatccgt cggtcgtgtt tgatgatatt gtcgaaaacg
    13621 aatacattac cgcccgcgcc gaagacattg cctgctatta cgatgactta atcgaataca
    13681 cccagtatta caacctgttg ggcgaagggg tgcacaatgt cggcggcaaa cccgttaccg
    13741 tgtctttgcg cgggttgaag aaaaaactct atctctgcct gatgtgcgtc aatgtgttgg
    13801 aagccatccg tttctacgtt tcattcgcct gctcgtttgc ttttgccgag cgcgagttga
    13861 tggaaggcaa cgccaaaatc atcaaactga ttgcccgcga cgaagccctg cacctgaccg
    13921 gcacgcagca tatgcttaat ctgatgcgtt cgggtgtcga tgattctgaa atggcagaaa
    13981 ttgccgccga gttgcaggac gaatgtttcc aactcttcaa aaaagcggcg gaacaggaaa
    14041 aagagtgggc ggcatatttg tttaaggacg gttcgatgat cggtttgaac aaagaaatct
    14101 tatcccaata cgtcgaatat attaccaatc tgcgtatgca ggcggtgggg ttgccggccg
    14161 gatttgaagg cgcaaatcaa aacccgattc cgtggattaa tgcgtggctg tcgtccgata
    14221 acgtacaggt cgcgccgcag gaagtggaaa tatcctctta tttgatcggt cagatcgatt
    14281 ctgaagtgaa tacggatgat ttgggcgatt ttgagttgta aacgtttttt gaaaaaatgc
    14341 cgtctgaagc cggatgtttc agacggcatt tgtatgttgt atgatgggaa aaacagcggg
    14401 taggatcggt tattgttcct tttatagtgg attaacaaaa atcaggacaa ggcgacgaag
    14461 ccgcagacag tacaaatagt acggcaaggc gaggcaacgc cgtactggtt taaatttaat
    14521 ccactatatt tttgattggt cggaaatggc gcgcattggt acgaataagg gtttttttga
    14581 gctcttggag ggcgagaccc tgttggaagg cttggaacgg acggggcata tggtggaata
    14641 tcagtgccga agcggatatt gcggatcgtg ccgagtgaag attttggagg gaagcgttac
    14701 ttatcgggag ccgcctttgg cttttttggg gcgggacgag atactgccgt gctgctgttg
    14761 cgtcgagggg gatgtcaggc tggattgcgg tttgtcgggg gaggatgagg ggctgtctga
    14821 cagtttgttg aaataaaagc cgtccgaaca ttggttcgga cggctttttg acggtattac
    14881 cggttatgcc tgatgagtcg gtaaaaggtt tcaccgtctt ggatataacc cagttctacc
    14941 cgggcgattt ccgaaatggc ttcttgaccg ttttccaaat catagacttc ggcggcaagg
    15001 gaatgattgc gtagggcgag tgtctggttt ttttcttctt gaacggcaat ctgttctctc
    15061 agactgctgt tgcgtccgat gctgcctttg ccgaaccaga ggctgtattg gcaacagaca
    15121 agtgcgaagg ataaaacgac agttacccac ttcatacctt aacctttttt atttgcccag
    15181 ttggtagaat gcggctttgc cggggtagtc ggcggcttcc gccaattctt cctcgatacg
    15241 cagcagttgg ttgtatttcg ccatgcggtc ggaacggctc aaagaaccgg ttttgatctg
    15301 catacagttg gtggcgactg ccaagtcggc aatggtgctg tcttcggttt cgccggagcg
    15361 gtggctcatc acgctggcgt agcggttgcg ttttgccaga tcgacggctt tcagggtttc
    15421 actcaaagta ccgatttggt tgactttgac caatagtgcg tttgccacgc ctttttcaat
    15481 gccttcggca aggattttcg ggttggtaac gaagaggtcg tcgccaacga gttggacttt
    15541 gccgcccagt ttttcggtca gcagtttcca gccttcccag tcgttttcat ccatcccgtc
    15601 ttcgatggag atgatgggga actcgttgac cagactttcc agatattcgg caaattccgc
    15661 gttggtgtag gagcggcctt cggcttccaa gtggtatttg ccgtctttgt agaactcgct
    15721 ggacgcgcag tccaatgcga ataatacgtc ttcgcccgct ttgtagccgg cgacttcggt
    15781 ggcttcaacc atcagttgca gggcttcttt gtggctgttc aggttggggg cgaaaccgcc
    15841 ttcgtcgccg actgtggtcg ggaagccttt gctgtcgcac agttttttca aggcgtggaa
    15901 aatttccgca ccgcagcgca acgcttcgcg gaaagatttt gcgccgacgg gcataatcat
    15961 aaactcttgg atgttcaggc tgttgttggc gtgttcgccg ccgttgatga cgttcatcat
    16021 cggtacgggc agggacatcg gacctgcgcc gcccaagtag cggtaaagcg gcagccctgt
    16081 atcttcagcg gctgcgcgtg ctacggccat agaaacggcc aaagtcgcgt tggcacccaa
    16141 gttacctttg ttttcagtac cgtccaattc gatcatgatt tggtcgatat aagattgttc
    16201 gctggcatcg ataccgatga gggcttgcgc gatttggttg ttgacgtggt caacggcttt
    16261 caatacgcct ttgcccaagt aacgggattt gtcgccgtcg cgaagttcta aagcctcttt
    16321 ttgaccggtg gacgcgccgc tcggtacggc tgcgcgtccc attacgccgg attcgagcaa
    16381 tacatcacac tcgactgtgg ggttgccgcg tgagtccaaa atttcgcggg cgaaaatatc
    16441 aacgattgcg ctcatgaatg ttctccaagt gaagtgaagg gaggtaaaaa tgggagggct
    16501 tcagacggcc tctttgccgg aaagccgcca acttaaaact gctgaaagta gggcggttgc
    16561 cataaaaaca atactggaaa tgccgtgcca cattccgaag gatccgccta caaacgacaa
    16621 cagccaattc tcatgcccgt atttcagcgc ctcgataacg ggggtaacca aaaattggtt
    16681 ggcggcaagg gcggacaata aaaaaagcag tagggcggtt tgccgccggg ttagggcggc
    16741 aaaggcggca gccagtaccg cgccccaaac ggcaagcccg ctccaagaga ggatgtcgaa
    16801 caatatgccg gcaatttcgc ccgcctgcat tttgggcagc attttgaaca gcaccggtgc
    16861 ggcgatataa cccgccataa tctgcatacc gagccacaag gttgcggcat accggctgat
    16921 ttttcggaaa gtctgcatca taaggcgaaa ccgtgttatt cgattgttaa aatcggttgt
    16981 gatttgatta aatcgtccaa tgctttgatg cggattaaaa aatcttctaa aaggtgcagc
    17041 ggcagcgcgc tggggccgtc gcattttgcc agtttcggat cggggtgcga ttcgaggaac
    17101 aggccggcaa ggcgggttgc catgcctgca agtgccaaat ccaaagcctg tgcgcgacga
    17161 ccgctggatg cggcagaacc ggcatcgcgg gtttgcaggg agtgggtaac gtcgaaaata
    17221 accggcaggt tgccgcaagt ctgtttcatc acgccgaaac cgagcatatc gacaacgagg
    17281 ttgtcgtagc cgaagctgct gccgcgttcg cataaaatca gtttcccgtt gccggcttcg
    17341 cggaattttt ccacaatgtt tttcatttga gaggggctga ggaattgtgg ttttttgatg
    17401 ttgatgacat tgcccgtttc cgccattgcg gccaccaaat cggtctgccg cgcaagaaag
    17461 gcgggaagct ggatgacatc gcacacttcg gcaacgggtt ggcactgata gggttcgtgt
    17521 acgtcggtaa tgacggggat gccgaactct gctttgactt tttcaaaaat ctttaagcct
    17581 tcttccaagc ctacgccgcg ataggaatgg atggacgagc ggtttgcctt gtcgaaagag
    17641 gctttaaaga tatagggaat actgagtttt cgggtaactt cgacgtaatg cgcgcaggtc
    17701 tggagggtgg aatccaaatc ttctaaaacg ttgatgccgc cgaataggac gaagggcgag
    17761 ttgttgccga tggtgatgtc gttgatttta atatccataa tcaatgtccg ttttcagggt
    17821 tacagtacgg tgtgcggcgg ctcgtaggag atgatatcgc ggattttgtt gtttccgtct
    17881 accaagacga ctttgggctc gtgtgcggcg atttcgggtt cggagagttg gacgtaagac
    17941 atgatgatga cgatatcgcc tttctgcacc agccttgccg cagcaccgtt cagacaaatc
    18001 acgccgctgc cgcgtttgcc tgaaatggta taggtttcaa aacgttcgcc gttgttgttg
    18061 ttgacgatgg cgactttttc gttgggatag atgcctgccg cgtccaacag gtcttgatcg
    18121 acggtaatgc tgccgacgta gttcaaatcg gcttcggtta cggtggcgcg gtggattttt
    18181 ccgccaagca tagtacggaa catgtatttc cttttggttt gtgtgcggtc tgacgggcgc
    18241 gatgctgtct gaaaatctga atgccgtatt atagtggagt ttccttcaaa ttcataatgg
    18301 cggctttcgg acaaggaaaa cggtgtttca gacagcattg cggtgtcggc tttatttggg
    18361 caggcatctg ttttgcaagg tttttaaaac gttttttacg gtgttgattc tgacctcgat
    18421 attttccatc tctgcggtaa accgcagctt atcggcgccg gcaaggcggt attttttgtc
    18481 gttctgaatc agcaggatga tttcggttgg atcaatattg ttgtttttgc caaaggttac
    18541 cgttaccgct tcgccggccg catcaatggc attgataccc aattcttttg ccataagccg
    18601 taagtggtgg ctttcgatga gggttttgac gggttgttcg ggcagtccga agcggtcgac
    18661 gagttcttcg tgtatggcgt tgatttgttg cacggtttcg cagacggcga ggcgtttgta
    18721 gaggacgagc cgttcgtgga tgtcggggca gtagctttcg ggcagtaggg cggggctgtg
    18781 cagtttgatt tcggtggtga tgcccagcgg cgcgtcgagg tcgggttggc ggcctttttt
    18841 gagatcgcgc acggcttgtt tgagcatttc ggtgtagagc gtgaagccga cctgtatcat
    18901 ttcgccggat tgtccttcgc cgaggatttc gcctgcgccg cggatttcca aatcctgcat
    18961 ggcgagggtg aaacctgcgc cgagttcgtc tgctgccgcg atggcgtcga ggcgtttttc
    19021 tgcgtctttg gtgatgtatt cgggcgtgag caggtaggcg taggcttggt ggtggctgcg
    19081 gccgacgcgc ccgcgaagct ggtgcagttg cgccaatccg aatttgtcgg cgcggttgat
    19141 gatgatggtg ttggcgttgg ggatgtcgat gccggtttcg atgatggtag agcagagcaa
    19201 cacgttgaat ctttgctgca aaaagtcgcg catcacttgt tccagctcgc gctcgcgcag
    19261 ttgtccgtgc gccacgccga tgcgggcttc gggcaacagg gtttccagcc gctcgcgcat
    19321 attctcaatc gtatctactt cattgtgcag gaaaaatacc tgcccgccgc gtttgagttc
    19381 gcgcaacacg gcttcgcgca cgctgccttc gctgaacggt ttgacaaagg ttttgacggc
    19441 gaggcggcgg ctgggcgcgg tggtaatcag cgagaagtcg cgcagacctt cgagtgccat
    19501 gctgagggtg cgcggaatcg gtgtggcggt catggtgagg atatcaacat tggcgcgcag
    19561 gcgtttgagc tgctctttct gacgcacgcc gaagcggtgt tcttcgtcga taatcactaa
    19621 acctaagttt ttgaatttga tgtcgtcctg caccagtttg tgcgtaccga taacaatatc
    19681 gaccgtgccg tctgccatgc cttccagcgc ggctttggtg gctttgctgt tgttgaaacg
    19741 cgaaaggctg gcgactttca cggggaaatc ggcgaaacgg tcggcgaagt tttgcgcgtg
    19801 ctgctcgacc aaaagcgtgg tcggggcgag tacggcaact tgtttgccac ccattaccgc
    19861 cacaaacgcg gcgcgcaggg cgacttcggt tttgccgaaa ccgacatcgc cgcacacgag
    19921 gcggtccatc ggcttcgcct gcgtcaaatc tttaatcacg gcggcgatgg cggctgcctg
    19981 gtcttcggtt tcttcgtagc cgaagccgtc ggcaaacgcc tgataatcca actcgttgat
    20041 ttcaaacttg tgtcccgatt gggcggcgcg ttgggcatag agattgagca actcggcggc
    20101 ggtatcgcgc gctttttcgg cagccttgcg ttttgccttg ttccacgcgc cgctgccgag
    20161 cttgtgcagg gcaatgtttt catgcgcctg accggagtag cggctgatta aatgcagttg
    20221 cgaaacaggc acataaagct gcgcttcgcc tgcatattcg agcaacatca tttcgttggt
    20281 ttcgccgccc aaatctatcg ttaccaagcc catataccgc ccgatgccgt gttcttcgtg
    20341 aacgacgggg tcgcggatgt tgatttcggc aaggtcgcgc aacagaccgt ctgaaacggc
    20401 ggcgtgtttc ttgcggcggt tgtggacgcg cgaacgggcg acgtattggt agagatcgga
    20461 ttcggtgatg acggcgatgg cactttgagc ggcaactgct ttgccctctc cccaaccctc
    20521 ccccacgggg gagggggcag gttgctgttg gatttgcccg tgcagattac ttttaccggg
    20581 gggcaggctg ctttcggttg aaacggcggc tgctttcaga ctgtctgaaa cgacggctgc
    20641 actttgttcc ctctcctgtg ggagagggct agggagaggg tttgttgcgg atgcggaaaa
    20701 ttcggtttgg tcggtaaccg catcgccttc tccttcggag gcggaagtag tttgctgttg
    20761 gttcggcgat tgcagtccgc ccagtttaaa cccgtaagcc aacggcgcca ctgtaatcat
    20821 cagcggctca tgcgccgaca agaagccctg ccagtcggac acaggtttgg ctttcaaacc
    20881 gttttgctgc aagaaaccga gcatggtttc gcgccgtccc aagctttcgg cgcacagcaa
    20941 aatccgcccg tcaaaggtcg tctgaaaatc cttcaatgcc tgcaacggct cgtcggattg
    21001 gcggttgacg gcaaggtcag gcagggtgtg ttccttgccg gaaacatcgg gcagcacttg
    21061 tccgtagttt ttcagacggc ctgcaaaaac atcagcagag agatacaaat gctgtggaag
    21121 caaaggcgga taggtttcgt cgccctgcgc catcgcgtaa cgggatttga catcgctcca
    21181 aaagcggttt gcctcggcgt gaacatcgcc caaagagaca aacagcgcgt cttcgccgat
    21241 atagtcaaac agcgtttcca gctcgttttc aaaaaacagc ggcagataat attccacgcc
    21301 cgcaccgaag tggccgttgc ttactgcttt gtacacagcc gcatcgttcg gattaccatc
    21361 gacttcctcg cggaagcggc tgcggaagat tttttgcgcc tcgctgtcgg tggggaattc
    21421 gtgcgccggc agcaggcgga tttcggaaac gggggagatg gtgcgctgcg tgtccgtatc
    21481 aaaggttttg atgctgtcga tttcatcgtc aaacaaatcg atgcggtagg gcatttcgct
    21541 gcccatgggg aacaaatcga caatgccgcc gcgcacagcg aactcgcctg ccgcaacaac
    21601 gtgggaaaca tggttataac ctgcatccac caaatcgctt ttcagacggc ctatatccaa
    21661 agtctgtcct gttttcagcc aaaacgtgcg ccccgccaaa aacggcacgg gcggcagctt
    21721 ctgcatcgcc gtggcaaccg gcacaaacaa cacgtccgcc gcgccgctct taatctgcca
    21781 caacgccgag agccgctccg acaccaaatc ctgatgcgga gaaaaacgct cgtaaggcag
    21841 cgtttcccag tccggcaaga acaccgccgt gtcgtgcgga cggaaaaacc gccatgccgt
    21901 ctgaaggcgc aacgcctgtt ccgcatcttg ggtcagcacg accttgagcc gcttgtgcgg
    21961 cagataacgc gccaaagcca agggcagcga gccttgcgaa agattgggcc aacgggattt
    22021 ttcacggggt ttggggatgg ggtaggtcat agtgcggcaa tcggcggaca gtgatgcaaa
    22081 cggtcatttt agcacgggag ggtgcgcgat gccgtctgaa aagccggcgg attgttgccc
    22141 ggcgcacttt gtggtaaagt ttagaaaatc aactctaaac tgtggcaggc ggtttgaccg
    22201 gccgccgatt aatgggaacc gacagaggaa gccgatgata cacaccgaac cgagcgcgca
    22261 gccgtcaact atggacacgg ctgctttttt aaagcacatc gaatccgcat tccgccgcat
    22321 tttttcagac ggtatcgacc tgatgcgata cctgcccgaa gacaaatggc ttgccttgaa
    22381 gcaggcgggt ttgctgttgc ccttcctcga caaaagacac ggcggccgca agggcagcca
    22441 gtttgaaatt caggaagtat tgcggattgc ggggcattac ggcgtacccg ttacgctgcg
    22501 taccggcatc gaaggcgcgc tggtgttgca gccactgcaa gagttcggcg atgaagcgca
    22561 aatcgcacag ggtttggaca tggttttcaa aggcgagggc ggcggtttgg gcgttaccga
    22621 acccgaaacc tccggcgcgg cgattgcccg agaaatgcag tcttactacg aatataccga
    22681 cggacaaacc atttacgtca acgccgcaaa atactggcag ggcaactcgc aaagcgactt
    22741 cctcctcgtt gccgccaaag aacgcaaaaa cggcaaactc gccaaagtca tcgacctgct
    22801 gctcgtcccc aaaacataca tccgctgcga aaccctcgca tccgaaggct tgcgcgccgt
    22861 ccgttacgcc gtcaaccgca tcgatgccga aatgcctgca accgccgtga tgaaactctc
    22921 ccagagcgac gccgcaggtt tgcgcgcgtt ccaaaacatc tttatccgca gccgcctgca
    22981 actgatcggc atgacgcacg gcattatgga atacatcctt gaaaacctgg aacgatacgt
    23041 ccgcaacgac atcagattcg tcgattacga acgccgcgaa atccggcgcc gccatcaggt
    23101 ttccgagatt ctttaccgct acgtctgcca ttccgtttcg cccgttgcac ccgtcgccca
    23161 tcagctgatg gaggcgaaca tcgtcaaaac cctcgccacg gaatacactt acgccgccgc
    23221 gcaaatgttg caaaaacttt tgggcgcgaa gggttttgaa cgcggacaca ccgccggcaa
    23281 tatcgctatc gacatccgcc ccttcacgat ttttgaaggc ccgaacgata tgctttatgc
    23341 cgaaatttac gaccagttcg tccgcgccac cgccgaagaa aaagaagcag gcatgaagtt
    23401 ggacaaaaac caaaccctgc tcgaccgcct gcaaaccgat gcccgctttg ccgccgtcgc
    23461 ccgcgactac actttgcccg aagacatccg cagcttcctg caggaacaca ccctgaccga
    23521 cgcctgcgcc ctgcaaaaag tcttcatcgg caaaatcatc gcccgactct ttgtcttcgt
    23581 acaggcggaa cacgaagaca ccgcagcctt cctgctgaac gacatccgca aagatatatt
    23641 ggactgccga tattgcggat agcgggcaaa tacggcggca tacggatttc aggcggtgca
    23701 aaaggcaacg cgcggtattc cacaaagccg ccgattgcgg cggatgccgc atttcgcacc
    23761 gcgccccttg cgccgctttc ccacttaccc tatttgttcg gaacatattt atgaaaaaga
    23821 gaaaaatact gccgctggca atttgtttgg cggctttgtc tgcctgtacg gcgatggagg
    23881 cacgcccgcc ccgggtaaat gaagcccaag ccccccgcgc ggatgaaatg aaaaaagaaa
    23941 gccgccccgc gtttgacgcg gcagccgtac cggtatccga cagcgggttt gccgccaatg
    24001 caaatgtccg ccgttttgtg gacgatgaag tcgggaaagg ggatttttcc cgggcggaat
    24061 ggcaggattt ttttgacaaa gcggcttaca aggcggacat cgtcaagatt atgcaccgac
    24121 cctccacatc gcgtccgtgg tatgtgttcc gcacgggaaa ttcgggcaag gcgaaatttc
    24181 gcggcgcgcg ccggttttat gcggaaaacc gcgcgcttat cgatgatgtg gcgcaaaaat
    24241 acggcgtgcc tgccgaactt atcgtggcgg ttatcgggat tgaaacgaat tacggcaaaa
    24301 atacgggcag tttccgtgtg gcggacgcat tggcgacctt aggctttgat tacccccgcc
    24361 gcgccgggtt tttccaaaaa gaattggtcg agcttttaaa gctggcaaaa gaagaaggcg
    24421 gcgatgtttt cgcctttaaa ggcagctatg cgggcgcaat ggggatgccg caatttatgc
    24481 cttcgagcta ccggaaatgg gcggtggatt atgacgggga cggacatcgg gacatatggg
    24541 gcaacatcgg cgatgtcgcg gcatcggttg ccaattatat gaagcagcac ggttggcgca
    24601 cgggcgggaa aatactggtg tctgcaacat tggcgccggg tgcggatgtt caggcaatca
    24661 ttggcgaaaa aaccgccctg acgcggacgg tggcggattt gaaggcgtac ggcatcatcc
    24721 ccggcgaaga gcttgccgat gatgaaaagg cggttttgtt caaactggaa accgcacccg
    24781 gcgtgtttga atattatttg ggcttgaaca atttttatac ggtatggcag tacaatcaca
    24841 gtcggatgta tgtaacggcg gtcagggaca ttgccaattc gcttggcggc ccgggattgt
    24901 aatgtttttt aaaatgccgt ctgaaccacg tttcgattca gacggcattt ttgcatcctt
    24961 ttatggggat ttcccgcatc agggcggtat tcggggcgaa tcccgcttca gacggcattg
    25021 ccggtcaagg cttgtccgaa ggaggatgtc cctgttcttt ggcgggcggg aggaaaagta
    25081 tgcagggatt gcttttaatc tgattccaaa aacttttaat cagattgcgg atgctgccga
    25141 ttttcgtgcc gcgcgagcgc agggcgacga agagggcgag gctgaaactg acgcagaggt
    25201 tgaccaggcc gatggcgagg acgctgaaaa tgccgagtac gaacgtgccc aaaccgacgt
    25261 tgccgctgac ggcggcatag ccgaggttgg cggaggaaaa ggcgacgtgg cggatgtcca
    25321 gcggcagccc gaggaggtgt ccgaaatagc cggtcatacc caagagcatc ccgaagatga
    25381 agttgcctac caacgagccg tagtgtttgt ggatgtaggc ggcgaggacg cggcggggcc
    25441 cggggcgcat gattttacgc agcaaggggt tgaaaggcag gcgttggcgc aggttgaggt
    25501 agtcggcgcg gttgtcgaaa aaacctgcga tgatgccgga gcagaacagc cacagacctg
    25561 caatggcggc ataccacagc gtcgggtaag cgatgatgtc tatggatttg aactggtagg
    25621 cggcggtgtg ggcatcgagt atgggctgcc ggtacagatg ggcatagccg aacgatatgg
    25681 cgcacgccaa aaggatggcg atggaaacgt tgccgaagac ggcgacgctt tgggagcggc
    25741 atacgtcgat gaggagcttg gcgagtttgt tgtccaccgc tttgccgcct tcgttgagat
    25801 cgacctgttc ggcaaagctg gcggcagtca tcgcgggctg cttggtggcg acggtgcagt
    25861 gcagcatatg gatgatcata aagccgatgc cgtagttgaa cccggccgac aaggaagtga
    25921 ggaaggggct gaggccgagt gtgccgatgc ggattttgag cagcgccatc agggcgatga
    25981 tgacgccgcc gcctgccgcc gagtagaaca ttttgaaata ttctttgcgg ttgcgggtga
    26041 tgtagtgttc gccgtggtgg cttttgtttt cggtaatgct gcgggcgagc atacggatgc
    26101 tgctgcggcg caggtggcgg gtgctgtatt gttcgaccgc cgcgtagatg agggagttca
    26161 tcagcgcgat ggtcaggcgg ttgctgttgc cggcgccggt ttggatgtcg gtgagcagct
    26221 tcagacggtc tacggtctgc cggagccgtt cgagcaggtg ggcgactttg accgacgaac
    26281 cggagccggc gcccgtccct ttgcggcgca aatagtcaat ctggctgaaa cattggtcga
    26341 acatcacctc gaggtgggcg gtgtcgtaag gcgcagtgct gttgcggtag tgttcgacca
    26401 gtttggcggt ttcgcgctgg agggcgacga aggaagaatc ggcttccagc aggcgcgggg
    26461 cgatgcggat gaggtcgggt tcgatggctt cggatgccgt ccagacagac agcatttcga
    26521 tggcgcgcaa acgcgcatcg gcaaggcgga tgcccgccgt ctgcaatttg gacggttcgg
    26581 cgtggcttcg gatgagttcg tataaaacca gccattggcg gatattgagc gtttgcagcc
    26641 atttgtcgtc gttttcggaa tggaaaagat aaaggaagac ttcgcccaag ttggcaaaat
    26701 ctttatatga cgggctgaag cgttcgtata tgcgtatgcc catttcccgg gcaaagctgt
    26761 ggcgcgagaa gatgccgagt ttgatgagtg ccggataaat gtgcacctgc gcgagccaga
    26821 tgtaaaaaca ccggctgaag cgggacagta attccctgtc ttgtttgaat gtgtcgataa
    26881 tcaggtcgaa acgttccccc gcacattttt tgccgccacg gcgcaaaaat ttaatcagtg
    26941 cgttgaggac gttgacaaaa tcggtatgtc ccaagctttc cgaaagcagg gggcgcaggt
    27001 tttgaggggt aattttcttc atatcagcca ttgtaccgca tcggcaatcc agcgttaaac
    27061 gccccgatgg cggggagggc gcgcctgcgg aaggcggcgc attttccgct agtttgaaat
    27121 caaactatat taatgatata ataaatatag ataaatcaat ctgatagaat aatccgtatt
    27181 gatttttaat tttttcgatt ataacaattt cggccggttt gtgcatttcg tcatccggaa
    27241 gccggtattt taccggcagg ttttgaccgc tttgcccgat gaccgggttt gtctgttttt
    27301 aacaaaggag tgagaaggtt tgtccctgtc tgaatttata gaacgccgaa cgtcatttaa
    27361 tccgatggtt attttgacga ctttgttttt tgtgtgtgtt ttggtggtat tggttttaac
    27421 cgtgccgaat caggtgcaga tgtggctcga tcgggcaaaa gaagtcattt ttaccgagtt
    27481 cagctggttt tatgttttaa cgttttccat ttttctgggt ttcctgctga tactctcggt
    27541 cagcagtttg ggaaacatca ggctcggacg ggatgaagat gtgccggaat tcggcttcct
    27601 gtcgtggctg gcgatgctgt ttgcggccgg gatgggcgtg ggtctgatgt ttttcggcgt
    27661 ggcagagccg ttgatgcatt atttttcgga cattacggcc ggcacgccgg aacacaggca
    27721 gcagcaggca ttgctgcaca cggtgttcca ttggggcgtt cacgcttggt cggtgtacgg
    27781 tacgattgca ttggctttgg cttatttcgg tttccgctac aagctgccgc ttgccctgcg
    27841 ttcttgtttt tatcccctgt tgaaagaaaa aatttccgga aggttcggcg atgccattga
    27901 tattatggcg ttgcttgtta cttttttcgg catcatcacc acattggggt tcggggcttc
    27961 gcaactgggc gccggattgc aggaaatggg ctggattgcc gaaaacagct tcagcgtgca
    28021 ggttttgatt atcgccgccg tcatgtccct cgccgtcgtt tcggcaatat ccggcgtggg
    28081 gaagggcgtg aaggtgttga gcgagttgaa cctgagcctt gcgtttttgc tgctgttttt
    28141 tgttttggcg gcgggtccca ctgtttacct gttgtcggca ttcggcgaca acatagggaa
    28201 ctacctcgga aatctggtgc gcctcagttt taaaacttat gcgtacgaac gggaacacaa
    28261 gccgtggttt gaatcttgga cggtgcttta ttgggcgtgg tggtgttctt gggcgccgtt
    28321 tgtgggtttg tttatcgcgc gcatttcaaa ggggcgcacc atccgcgagt ttgtcttcgg
    28381 ggttttgctc atccccggcc tgttcggcgt tttgtggttt accgtcttcg gcaatacggc
    28441 gatttggctg aatgacgggg ttgcgggggg aatgctcgaa aagatgacct cctctccgga
    28501 aacgctgctt tttaaattct ttaattacct ccccctgccc gaattgacga gcatcgtcag
    28561 cctgctggtc atttctctgt tttttgtaac ttctgccgat tccgggattt atgtcctgaa
    28621 caatattacc tctcgggaca aaggcttgag cgcgccacgg tggcaggcgg ttatgtgggg
    28681 cgtgctgatg tctgccgttg ccgttttgct gatgcgctcg ggcggactcg gcaacctgca
    28741 gtctatgacc ctgattgttt ccctgccgtt tgccctgctg atgctgataa tgtgtttcag
    28801 cctgtggaaa ggcttgagtg cggataagaa atattttgag acccgggtta accctaccag
    28861 tgtattttgg acgggcggca agtggaaaga acggctggtg cagataatga gccagacgca
    28921 ggagcaggat attttaaaat tcctcaaaca taccgcatcg cccgctatgc acgagttgca
    28981 acgggagctt tcggaagaat acggcttgag cgtccgggtc gataagatgt ttcatcagga
    29041 cgagcccgca atcgagttcg tcattcggaa agagacgatg cgcgatttta tgtacgggat
    29101 taagtctgtc gggcaggatg tatccgacca gttgattaac gacggcaagc tgccgcatat
    29161 ccggcatcag acaacttaca aaccctacgc ttattttttc gacgggcgcg tcgggtacga
    29221 tgtgcagtat atgaacaagg acgagctgat tgccgacatt ttgaaaaact acgaacgtta
    29281 tttgatgttg ttggatgatg tcggtcagga actgatggcg cacgagcagg tggaattggc
    29341 agagtaagtg ccttatctct ggcgtttgcc aaaaaaaatg cggtgaatca gattcaccgc
    29401 attttttttg ccggacgggg cttcggacgg cacggcgttc atttgtttcc gtcaaactgc
    29461 tccttcaaca cccgtttcaa taccttgccc gtagcgttgc gcggcagccc gtctttaaag
    29521 tggatttgtt tggggatttt gaaatttgcc agcacggtac gcaggtggcg gcggatttcg
    29581 ttctcgccca aatccatacc ttccttcaat tggacgaagg cgacgatttc ctcgtcggca
    29641 taacggtctt tcacgccgat gacggcggcg gcttcgacgg catcgagttt gtagatttct
    29701 tcttcgattt cgcgcggata gacgttttga cctttggaaa taatcaaatc ttttttgcgg
    29761 tcgacgataa agataaagcc gtcttcgtct atggtaacga aatcgcccgt tttcaaccag
    29821 ccgttgacga tggtttcatc ggtggcggca ggcatattga ggtagccccg catcaccgaa
    29881 ccgcccctga cgatcagttc gcccacttcg ccgcgcggca cttcgaccaa ttcttcatcg
    29941 acggctttgg cttccaaacc gggcaggggg atgccgacgc tgcgggcttt ttgcctctcg
    30001 ggcgtattga cggcgacgac gggagaggct tcgctcagtc cgtagccttc cagcaatttg
    30061 gcgcgaggga acttggcttt gaaatcgagg atggtttgtt ccgccaaagg cgcaccgccg
    30121 ctgataaaca ggcgaatgcg gttgaaccat ctgaaatacc aagggatttt cgccttgctc
    30181 attgcggtgt aaatcgcggg tatgcccaaa aacacggtcg cgcgtttgag cagggcctgt
    30241 ttcaaaacgt tggaaaacgg aaaaacggat ttgaccaaaa taatcgaaca cgccatataa
    30301 atcggcagca gcaccatagc cgtcagcgtg aagctgtgga acatcggcag gaaaacgata
    30361 aagcggtcgc gtttggagat tttaaagatg cgttcgatgc cgttcaggtt ggcgaacagg
    30421 ttggcgtagc tgattagcgc acctttggga tgccccgtcg tgccggaggt gtagatgatg
    30481 tgtgccaaat catttatccg gggttggcgg cccaagtcgg gtttttcggg gaagcggcgc
    30541 acgtcttcaa aaaaggcatc gccttccgcc atttcgccgt ccggacggct ttggcccgtc
    30601 caaatgattt tttcgacggg cgtttgcgcc ttcaagcccg ccaattcttt tgacaggccg
    30661 gccgaggcga acaggaagcg cgccttgcag tcgttcagga tatacgcgta ttcgctgttt
    30721 ttcaaaaatg tgttcatcgg tacggcaacc gcgccgatgg cggatacggc gaaataggcg
    30781 gtaataaatt ccgtggaatt ggaaaccgcc agcgcgaccg tgtcgccgaa cttcacgccg
    30841 atattttgca gatacgccgc aacggcttcg gcttcctgct tgagcgcgcg gtaggcggtt
    30901 ttttccttgc cgtcgaacac tgccgtgccg ttgccgtttt tgcggcaggc ggcggtcagc
    30961 atttcgtaga aattggcata agtccggttc atgaaacttc ctttatggtt gcggacggag
    31021 aacgccctca tttgaaaaca atatgccg
//
LOCUS       DAXHTY010000015        30289 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXHTY010000015 DAXHTY010000000
VERSION     DAXHTY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.015"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(13..399)
                     /locus_tag="ACFNMK_000564"
     CDS             complement(13..399)
                     /locus_tag="ACFNMK_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC6363889.1"
                     /translation="MRKILLDTHALLWWLLDDKELGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(396..644)
                     /locus_tag="ACFNMK_000565"
     CDS             complement(396..644)
                     /locus_tag="ACFNMK_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFC6363890.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLTGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
     gene            complement(1007..3385)
                     /locus_tag="ACFNMK_000566"
     CDS             complement(1007..3385)
                     /locus_tag="ACFNMK_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFC6363891.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVFAADEAATETTPVKAEVKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIEIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLDDRQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGNRGYPVEGAGKETNIRGSARGIPDPSKHKYHSFLGKIAYQINDNHRIGASLNG
                     QQGHNYTIEESYNLLASYWREADDVNRRRNTNLFYEWTPESDRLSMVKADVDYQKTKV
                     SAVNYKGSFPIEDSSTLTRNYNQKDLDEIYNRSMDTRFKRITLRLDSHPLQLGGGRHR
                     LLFKTFASQREFENLNRDDYYFSGQISRTTSSIQHPVKTTNYGFSLSDQIQWNDVFSS
                     RAGIRYDHTKMTPQELNADCHACDKTPPAPNTYSGWSGFAGLAAQLNQAWRVGYDITS
                     GYRVPNASEVYFTYNHGAGTWLPSPNLKAERSTTHTLSLQGRGEKGTLDANLYQSNYR
                     NFLSEEQKLTTSGDVSCTQMNYYYGMCSNPYSEKLEWQMQNIDKARIRGIELTGRLNV
                     DKVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLT
                     YLGAKKVKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPAKNLTLRAG
                     VYNVFNRKYTTWDSLRGLYSYSTTNAVDRDGKGLDRYRAPSRNYAVSLEWKF"
     gene            complement(3560..4189)
                     /locus_tag="ACFNMK_000567"
     CDS             complement(3560..4189)
                     /locus_tag="ACFNMK_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC6363892.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPYE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALGLSPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            4528..5988
                     /locus_tag="ACFNMK_000568"
     CDS             4528..5988
                     /locus_tag="ACFNMK_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HFC6363893.1"
                     /translation="MGSAVGGQPLEKIFLPDTKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPQLDRGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMAVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            5981..7642
                     /gene="pqiB"
                     /locus_tag="ACFNMK_000569"
     CDS             5981..7642
                     /gene="pqiB"
                     /locus_tag="ACFNMK_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC6363894.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            7642..8160
                     /locus_tag="ACFNMK_000570"
     CDS             7642..8160
                     /locus_tag="ACFNMK_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC6363895.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            8164..8715
                     /locus_tag="ACFNMK_000571"
     CDS             8164..8715
                     /locus_tag="ACFNMK_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC6363896.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWTLMLKKQQAFQTAFERFDIDTVAAFDEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDTHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            8705..9346
                     /locus_tag="ACFNMK_000572"
     CDS             8705..9346
                     /locus_tag="ACFNMK_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC6363897.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(9398..9622)
                     /locus_tag="ACFNMK_000573"
     CDS             complement(9398..9622)
                     /locus_tag="ACFNMK_000573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC6363898.1"
                     /translation="MPSEPAGRLFQKTPYRCNFMRLYGVGFALQTAYASRSSLVFQSV
                     HDYSGLNLNQYGVASPCRTICTVCGFVALS"
     gene            9621..9980
                     /locus_tag="ACFNMK_000574"
     CDS             9621..9980
                     /locus_tag="ACFNMK_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363899.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            10011..12863
                     /gene="gcvP"
                     /locus_tag="ACFNMK_000575"
     CDS             10011..12863
                     /gene="gcvP"
                     /locus_tag="ACFNMK_000575"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC6363900.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNMINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVSADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDAFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPETQTAGYRELLTDMENSLKAITGFDAISLQPNSGAQGE
                     YTGMLSIRRYQEANGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDTLSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKDDNPLVNAPHTAADITGDWARPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(13328..14167)
                     /locus_tag="ACFNMK_000576"
     CDS             complement(13328..14167)
                     /locus_tag="ACFNMK_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC6363901.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            14452..14527
                     /locus_tag="ACFNMK_000577"
     tRNA            14452..14527
                     /locus_tag="ACFNMK_000577"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14485..14487,aa:Arg,seq:ccg)
     gene            complement(14592..15785)
                     /locus_tag="ACFNMK_000578"
     CDS             complement(14592..15785)
                     /locus_tag="ACFNMK_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC6363902.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVRRAETARAATPAPSPYLPMEGLDTYRSAVQHLLFGTDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(15806..16894)
                     /gene="trmA"
                     /locus_tag="ACFNMK_000579"
     CDS             complement(15806..16894)
                     /gene="trmA"
                     /locus_tag="ACFNMK_000579"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC6363903.1"
                     /translation="MSDYTQQLQDKKDRLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASMIRCDRFEAASEAVNRLMPELIAAAAQSPELKKRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSISAAQWNIEANRIGNIKIARLSAEEFTEAYTRKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            17041..18141
                     /gene="aroC"
                     /locus_tag="ACFNMK_000580"
     CDS             17041..18141
                     /gene="aroC"
                     /locus_tag="ACFNMK_000580"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC6363904.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLALTESDIQAD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGCEHISQNPFFAANQSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFGSVVQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGDPVELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            18232..18657
                     /locus_tag="ACFNMK_000581"
     CDS             18232..18657
                     /locus_tag="ACFNMK_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC6363905.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAVAETPSVEAEAAESSAAE"
     gene            complement(18731..20716)
                     /gene="parE"
                     /locus_tag="ACFNMK_000582"
     CDS             complement(18731..20716)
                     /gene="parE"
                     /locus_tag="ACFNMK_000582"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC6363906.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDGGTEVRVWPDGKYFESPNYSILELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(20783..21307)
                     /locus_tag="ACFNMK_000583"
     CDS             complement(20783..21307)
                     /locus_tag="ACFNMK_000583"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC6363907.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIEFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            21473..22768
                     /gene="serS"
                     /locus_tag="ACFNMK_000584"
     CDS             21473..22768
                     /gene="serS"
                     /locus_tag="ACFNMK_000584"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC6363908.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(22846..23844)
                     /locus_tag="ACFNMK_000585"
     CDS             complement(22846..23844)
                     /locus_tag="ACFNMK_000585"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6363909.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAHDPFCNPEAEKIGGRYVDLDELYAQSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSSAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(23969..25045)
                     /gene="prfA"
                     /locus_tag="ACFNMK_000586"
     CDS             complement(23969..25045)
                     /gene="prfA"
                     /locus_tag="ACFNMK_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC6363910.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKINTLDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITNALIAEHQAELLAAMGD"
     gene            complement(25135..25905)
                     /locus_tag="ACFNMK_000587"
     CDS             complement(25135..25905)
                     /locus_tag="ACFNMK_000587"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6363911.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPQQRERQIQVFSRQLEIAQTLRRRVIIHNLKAAADIAAAVKRTGFTWGGIVHA
                     FSGSIEEAHVLAKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKNTV
                     NTPANILRIAEIAAQIRGTGAAEIAAITERNADSLLRP"
     gene            complement(26020..27009)
                     /locus_tag="ACFNMK_000588"
     CDS             complement(26020..27009)
                     /locus_tag="ACFNMK_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC6363912.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDTADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVRDFTRQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGAEDVEKELARLKL"
     gene            complement(27076..27759)
                     /locus_tag="ACFNMK_000589"
     CDS             complement(27076..27759)
                     /locus_tag="ACFNMK_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6363913.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(27948..29282)
                     /gene="glmM"
                     /locus_tag="ACFNMK_000590"
     CDS             complement(27948..29282)
                     /gene="glmM"
                     /locus_tag="ACFNMK_000590"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC6363914.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(29412..30263)
                     /gene="folP"
                     /locus_tag="ACFNMK_000591"
     CDS             complement(29412..30263)
                     /gene="folP"
                     /locus_tag="ACFNMK_000591"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC6363915.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNARTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSSEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVIMEKALALGGIDIINDVAALTDEGAVELLARQTDTGICLMHMRGLPENMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMVGELTGETDAAARVHGSVAAAVAAVARGAQIVRVHDVKATADALKVW
                     EALGINL"
BASE COUNT         6888 a         8334 c         7901 g         7166 t
ORIGIN      
        1 tttttgtatt tgtcagcttg atgcgttgac aactctaatt ccatactgcg gaatatattc
       61 atcgacagtc atcagttcaa agccttccgc ttgggtttgt gcaatcaaca tcctatcgaa
      121 agggtctttg tgtatctccg gaaggcttcc agcctgtttt gcatgaaaca gacctatagg
      181 caacatttca aaatcctctt cttgaagcac atcaaaaaac tcttccggta atttcaacaa
      241 ccccttgttc tgcttgatgg aaatttccca aatactcgct gcactgacaa agatcgcatt
      301 tctcggattt tctatcagtt tgcgtgcaga tatccccagt tccttgtcat ccaacaacca
      361 ccacagcaaa gcatgggtat caagcagaat ctttctcaca gagccgactc ctcaaaaaat
      421 aaagctgccg tttcattgtc atcctcaaga atacgtgaaa tatccgtatt ttccatatga
      481 ctgaattttt tcaaccttcc tgcatttcgt gccggttttt caataccggt tagttggacg
      541 caaggcttac ctgccttcgc aataataaca atttcccctg cttctgctct ttgaatcaat
      601 tgactcaaat tggtttttgc ctgatgaata tttgcttgaa acataacact tctcatgatt
      661 agctaactta actaatatac atcattacca agattttggg aatctcatta catatatttg
      721 attatatccg ccgttttatt cacaccttgc tatttatagt ggattaacaa aaaccagtac
      781 ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctgaag caccaagtga
      841 atcggttccg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
      901 aaaccccatc acacagattt acggtaaaaa accgtccgaa tgagttcttg aacacaattc
      961 ggacggcttt aattttcaac aaggcaatta attcaataat accagattaa aacttccatt
     1021 ccagcgatac ggcgtaatta cggcttgggg cgcggtagcg gtctaagcct ttaccatcgc
     1081 ggtcgaccgc attggtggtg ctgtagctat acaggccgcg cagggaatcc caagtggtgt
     1141 atttgcggtt gaacacatta tatacgcctg cacgcaaagt caggttttta gccggtttgt
     1201 agaagccgta catatcaaac acataagccg acttgttcag ccacgggtaa tcttttacct
     1261 ttttctgcaa aggcgtaccc cagcccttgt tttcataaac ggtgtattgc gcgtctttga
     1321 cctttttcgc gcccagatag gtcaggcggg agaacacgcc ccatttttcg ctcggacttt
     1381 catagtcgat accggcaatc actttcaacg gctgtgtgga cagcaggctg ttgtcgcccg
     1441 acagtttgct tttcgcataa cccagcgagc cgaacagttt ccagccctca ggaacaaaag
     1501 acgctacttt gtccacattc agacggcctg tcagctcgat accgcggatt ctggccttgt
     1561 cgatattttg catctgccat tccagttttt cggaataagg attgctacac ataccgtagt
     1621 agtaattcat ctgagtacag ctgacatcgc cgctggtggt cagcttctgc tcttccgaaa
     1681 ggaaattgcg gtagttgctt tgatacagat tggcatccag cgtacctttt tcaccgcgtc
     1741 cctgcaaaga cagggtgtgg gtggtgctgc gctcggcttt cagattagga ctaggcagcc
     1801 atgtgcccgc accgtggttg taagtgaaat acacttcgga cgcattgggg acgcgatagc
     1861 cggaagtaat gtcgtaaccg acacgccaag cctgattcag ttgagccgcc aaaccggcga
     1921 aaccgctcca gccgctgtaa gtattcggtg cgggcggggt tttgtcgcag gcatgacagt
     1981 cggcattcaa ttcctgaggc gtcattttgg tgtggtcgta acggatacct gcgcggctgc
     2041 tgaacacgtc gttccattgg atttggtcag acagcgagaa accgtagttg gtggttttca
     2101 cagggtgttg aatcgagctg gtggtacgag atatctgacc actgaagtag taatcgtcgc
     2161 ggtttaagtt ttcaaactca cgttgactgg caaaggtttt gaacaacagg cggtgtcgcc
     2221 cccccccgag ttgcaacgga tggctgtcca aacgcagggt aatgcgtttg aagcgggtat
     2281 ccatactgcg gttgtagatt tcatccaagt ccttttgatt gtagttacgt gtcaaggtgg
     2341 aagaatcctc tatcgggaac gaacctttgt agttgaccgc agatactttg gttttttgat
     2401 aatcgacatc cgcttttacc atagacaacc ggtcggattc cggcgtccat tcgtaaaaga
     2461 ggttggtgtt acgccgtctg ttgacatcgt cagcttcacg ccaataagaa gcaagcaggt
     2521 tgtaagactc ttcaatcgtg taattatgcc cctgctgacc gttgagcgat gcgccgatgc
     2581 ggtggttgtc gttgatttga taagcaatct tacccaagaa gctgtggtat ttgtgtttgg
     2641 acggatcggg gatgccgcgg gcggaaccgc ggatattcgt ttctttaccc gcaccttcta
     2701 ccggatagcc gcggttgccc gcgctttcgg tttcatggcc gcgccgttgc gaatacagca
     2761 aagcagcatc cacgcggtcg ttgctcacgc cgaaaccgag ggtatttgtc cattcacggt
     2821 tacgcgtgct gtaaccgttt ttcatcatca cgccgaattg cctgtcgtcc aacagcaaat
     2881 cacgtccttg cagggtttgg taattcacac cgccgcccaa tgcaccactg ccggtattga
     2941 aagagtctgc gcccttcacg atttcaatat tgcgtacgag ttcggggtcg atagacaaac
     3001 gcgagctgtt gaagttgcca taacgggcgt acagcgagtt ttcttcggaa tcaggcaggt
     3061 ttacaccgtc tatgctcacg ccgacacggt tgccttccac gccgcgaaca gcaaagcctt
     3121 tttgatggcg gccgctgtcg ctcaagccga catcggtgga atagcgcacc aagtctttgt
     3181 tgtcgcgtat catttcttgt ttgatacggt taaggttgac gcgttccaca gccgcaggcg
     3241 cattgcgctg gcctttaacg cgcactgctt ttacctctgc cttaacgggt gtggtttcag
     3301 ttgcagcttc atctgccgca aagaccggat tgccgaaaat actgccgacc agcgcggcga
     3361 tagggagcat ttgtaatggt ttcatattat caactcaaga tgtatggatt gttcatccat
     3421 cggttagcga ataatagatt ttatgataat cattaatatt taataagaca gtaatccatg
     3481 taaacaaagc cgcgccgtgt aaataaaggc ccctgcaaac agctatgccg agaccttgtt
     3541 tatttggttc gattattttt tagtgcctgt gcggcatcat tccttccggt gcttcggcat
     3601 cggctgccaa gccgaaggtt tcgcgcaaca cgactttgta gaatgcaaag gcttcgcgcg
     3661 cgccttggat ggcttcggct tcggcttcgg ggcttaagcc caatgcgttc agatgctcga
     3721 cgaaggcgcg ccagtgtttg ccgcgcccgt cgggatgcgg agcgaggtgg cgcgcgccgt
     3781 gttcgccgtt gtaatcgagt ttttgggcgt gtttgaacaa aaacgccgcg cccaaattgg
     3841 atccttcggc gcaataaagc cagccgattg ctttgttgcc agtttcgtat ggcaattctt
     3901 tgtcgaattt gtaaggctct tcgcccaaat ctttcaagtc ttgcgttacg gcatcgtatc
     3961 gcgccatgta ttccagctcg ggaatggctt tgtttaattc ggcatcttta tagatgtggt
     4021 cgacggcctt gtggaaaacg gattggagtt tcaaaaattt gatgtagttt tctttgctga
     4081 caaacggttg gacagacata acgaggttat ccacgctgtc gtgaaccgcc gtggtatccg
     4141 ccttcagacg tttggcaaac gtcagggctt gattttcggt ttcactcata agttttcctt
     4201 tgtcggtaag ggataaggat gcggggcggg caaaatccgc acttgcccgc atattatagt
     4261 ggattaacaa aaaccagtac ggcgttgcct cgccttagct caaagagaac gattctctaa
     4321 ggtgctgaag caccaagtga gtcggttccg tactatttgt actgtctgcg gcttcgccgc
     4381 cttgtcctga tttttgttaa tccactataa aaataataaa gaatcataaa cgaaatttat
     4441 tatcacatat ttttggaaaa aatatcattt tcgtgatgtt tttaagcagg tattttacta
     4501 ttctttacag aatcgggatt ttatcaaatg ggttcggcag tcggcggaca gccgctcgaa
     4561 aaaatatttt tgccggacac caagggtttg ttcatactgc cgaacctgcc ggttttgcat
     4621 cctgattggg tgtatcgcct tttttccttt ataatgccgc cacttatatt tgccactttc
     4681 ccgatgaagc cgtttgccga aaatatcccc cacagccttc gcggcaactg ccgcgatgaa
     4741 gccctgccgc cgcatacggt agattgcccg gaatgcggct gccgcgcgga tgtgccgcaa
     4801 ttggacaggg gggaggcggc gttctgcccg cgctgcggct ataagctctt cagggtgggc
     4861 aggcatcctt tttccgcccc gcccgcctat gcggcggctt cgctgatttt aatggcgttt
     4921 gcttacggta tgacgtatat cgaggtcgga ataccgggtg cggcatccgt cctttcgctg
     4981 cccgagatga tgcgcctgat ggtgtttcag gattatggtt ttttggccga agtgatgttt
     5041 gtgctgacct tcggcgcgcc ggttctgttt ctgctgctgt gcctgtatgt ctatgccgcg
     5101 ctgatacgga aacaggcgta tcctgcgctg cgtttggcaa cgcgtgtgat ggtgcgcttg
     5161 agacaggcga tgatggtgga tgtgtttttt gtttccactt tggtggcgta tatcaagctc
     5221 tcgtctgtgg cagaggttcg cttcggatcg gcgttttatc tgatgttcgc gctgtcggtt
     5281 atgctgattc ggacttcggt atcggttccc cagcattggg tgtattttca aatcgggcgg
     5341 ctgacggggg ataatgcggt tcagacggca tcggaaggta aaacctgttg cagccgctgc
     5401 ctgtatttcc gcgacagtgc cgaatccccc tgcggcgtgt gcggtgcgga actgtaccgc
     5461 cgacggccga aaagtctgag tatttcgtcg gcgtttctga cggcggcggt tattttgtat
     5521 ttccctgcca atatcctgcc gattatgatt tcgtccaatc ctgccgccac ggaggtcaat
     5581 accatcctta acggcatcgc ttatatgtgg gacgagggcg acaggctgat tgcggcggtt
     5641 attttcagcg cgagtatttt ggtgccggtg ctgaagattg cggcaatggc ggttctgatt
     5701 gcgtccgccc gcttcgcttt gccgacaggc gcaaagaaat tgtcgcacct ctaccgcatt
     5761 accgaagcgg tcggccgctg gtcgatgatt gatatttttg tgattattat tttgatgtgt
     5821 tcgttccaca cttatgccgc gcgcgtcatt ccgggcagtg cggcagtcta tttctgcctg
     5881 gtcgtgattc tgacgatgct gtccgcctat tatttcgacc cgcgcctgct ttgggacaaa
     5941 cgcgcttcag acggcattgc tttcaacgaa acggaaaaat atgactgaca acagccctcc
     6001 tccaaacgga cacgcccaag cacgcgtccg caaaaacaac accttgctct ccgccgtctg
     6061 gctggttccg ctgatcgcgc tgattgccgg cggctggctt tgggttaagg aaatccgcaa
     6121 cagggggcct gtggttacgc tcttgatgga cagcgcggaa ggcattgagg tcaacaatac
     6181 ggtcatcaaa gtattgagca tcgatgtcgg acgcgttacc cgaatcaaac tgcgcgacga
     6241 ccaaaaaggc gtggaagtaa ccgcccaact caatgcggac gtatccggcc tcatccgcag
     6301 cgatacccag ttttgggtgg tcaagccgcg tatcgaccaa agcggcgtaa ccggtttggg
     6361 tacgctgctt tcgggttcgt acatcgcctt tacacccggc aaaagcgacg aggcaaaaga
     6421 cgtgttccaa gtgcaggaca ttccgcccgt taccgccatc gggcaaagcg ggctgcgctt
     6481 gaatttgatt ggtaaaaacg accgcatcct caacgtcaac agccctgttt tgtatgaaaa
     6541 ctttatggtc gggcaagtcg aaagcgcgca tttcgacccg tccgaccaaa gcgtgcatta
     6601 caccatcttc atccaaagcc ccaacgacaa actgattcat tccgccagcc gtttttggct
     6661 ggaaagcggc atcaatatcg aaaccacagg cagcggcatc aaactcaatt ccgcccctct
     6721 gcctgccctg ctgtcgggcg cgatttcatt tgattcgccg aaaaccaaaa acagtaaaaa
     6781 cgtcaaaagc gaagacagct tcacgcttta cgacagccgc agcgaagtcg ccaacctgcc
     6841 tgacgaccgc tcgctgtatt acaccgcgtt tttcaaacaa tccgtgcgcg gactgaccgt
     6901 cggttcgccc gtcgagtaca aagggctgaa tgtcggcgtg gtttccgatg tcccttattt
     6961 cgaccgcaac gacagcctgc atctgtttga aaacggttgg attcccgtac gcatccgcat
     7021 cgagccttcc cgtttggaaa tcaatgccga cgagcaaagc aaagaacatt ggaaacaaca
     7081 atttcagacg gccttaaaca aaggcctgac cgccaccatc tccagcaaca acctgctgac
     7141 cggcagcaaa atgattgagt tgaacgatca gccttcgtct tcgcccaagc tgcgaccgca
     7201 taccgtttat gcaggcgata ccgttatcgc gacccagggc ggcggtttgg acgatttgca
     7261 ggtcaaattg gcggatttgc tggacaagtt cgacaaactg cctttagata agacggttgc
     7321 cgaattgaac ggttcgcttg ccgagctcaa atccacactc aaatctgcca atgccgccct
     7381 aagctccatc gacaaactgg tcggcaaacc gcagacacaa aacattccga acgaactgaa
     7441 ccaaaccctg aaagagttgc gcacaaccct gcaaggcgta tcgcctcaat cgcctatcta
     7501 cggcgacgta caaaatacgc tgcaaagttt ggacaaaacc ttaaaagacg ttcaacccgt
     7561 cattaacact ttgaaagaaa aacccaacgc gctgattttc aacagcagca gcaaagaccc
     7621 tatcccgaaa ggaagccgat aatgcgcctc ttccccatcg ccgccgccct gacgcttgcc
     7681 gcctgcggta ctgtgcaaag cacacaatat ttcgtgttgc ccgacagccg ctacatccgc
     7741 cctgcaacgc aaggcggcga aaccgccgtc gaagtccgcc ttgccgaacc gctcaaacgc
     7801 ggcggactgg tctatcaaac cgacccctac cgcctcaaca ccgcacaaaa ccacgtttgg
     7861 gcggacacct tggacgatat gctcgaagcg gcgttgagca atgcattcaa ccgtttggac
     7921 agcacacgca tctttgttcc tgcctcacgc agcggcagta ccgaaaaatg gacggtctat
     7981 attgacgcat tccaaggcag ctacacgggc aaaaccctca tcagcggcta cgccgtccta
     8041 cccgacggca cgaacagacc cttccatatc gaaaccgaac agcagggtga cggctacgcc
     8101 gcgatgaccg ccgcactcga acagggacta aaacaggcgg cgcaacagat ggtcgagtaa
     8161 accgtgaact attgcgaatt tgccgcctca cttcccgaaa acaccgacaa cccgaacaaa
     8221 cattatcacg acacgcaata cggttttccg attgaggacg acaatgaatt gtttgagcgg
     8281 ctggtgttgg aaatcaatca ggcaggattg agctggacgc tgatgctgaa gaagcagcag
     8341 gcatttcaga cggcatttga aaggttcgac atcgatacgg ttgccgcatt tgacgaagcc
     8401 gatattgagc ggctgcttgc cgatgcgggc atcgtccgca accgcctgaa aatcgatgcc
     8461 gccattttca atgcacggca aatccaagcg ttgcaacaag aatacggctc gttcaagaac
     8521 tggctcgaca cgcaccatcc gcgaagcaaa gacgaatggg ttaaactctt taaaaaacat
     8581 ttcaaattcg tcggcggcga aatcgtcggc gaatttctga tgagtaccgg ctacctcaaa
     8641 ggcgcgcacg ccgaaagctg tccggtttac cgtgaaaccc tgaaatacca cccgaaatgg
     8701 ctcgatgcca tctgaaaaac caatgaacag aagaaccttc ctcctcggcg caggcgcgtt
     8761 gctccttacc gcctgcggca gaaaatccgc ccgaacccac gccaaaattc ccgaaggaag
     8821 caccgtactt gccttgggcg attcgcttac cttcggctac ggcgcaaacc ctggcgaatc
     8881 ctaccccgcg caactgcaaa aactgacggg ttggaatatt gtcaacggcg gcgtatcggg
     8941 cgatacatcc gcccaagccc tgtcgcgcct gcccgcgctg ttggcacgca aacccaagct
     9001 tgtgattgtc ggcataggcg gcaacgactt tctgcgcaaa gttcccaagg agcagacccg
     9061 cgccaatatc gcgaaaatca tcgaaaccgt gcagaaggaa aacatccccg ccgtcctcgt
     9121 cggcgtgccg cacattacct tgggcgcgtt gttcgggcat ttgagcgatc atccgctgta
     9181 tgaggatttg tccgaggaat acggcattcc gctgttcggc ggcgcgtggg cggaaatttt
     9241 gggcgataat aatctgaaat ccgaccaaat ccacgccaac ggcaaaggct atcggaaatt
     9301 tgccgaagat ttgaatcaat ttttgagaaa acaggggttt agataaacaa aggtttatcc
     9361 gcacccaagt tgtttatata tagtggatta acaaaaatca ggacaaggcg acgaagccgc
     9421 agacagtaca aatagtacgg caaggcgagg caacgccgta ctggtttaaa tttaatccac
     9481 tataatcatg aaccgactgg aacaccaaac tgcttcggga cgcatatgcc gtctgaagtg
     9541 caaaacctac gccatacagc cgcatgaagt tgcagcggta tggcgttttt tgaaaaagac
     9601 ggcctgccgg ttcagacggc atgaccgacc gtccgagcct gctgtggata aagcccggac
     9661 aggctgaaat catggaatat tgcgaacctg aagaagcatc cgacccgtac gcaacataca
     9721 ggcgtgccaa cctgatggcg ggtctgccgc tgtttgtcgt gattttggtt ctgctcaata
     9781 ttgtttttcc gcttccggcg tatccattgg cttggctggt gcctgcaggt ttcatggttt
     9841 tgggcggcgg ctttccctta tcgctgccgc ttgtggcgct gcttgtcctg acctgctgca
     9901 ttctggcgca ttgtccgcca ttatcccgtc ttttgtgcta cccttgcccg aatcatccga
     9961 tgtctaaaaa ttctgcctga tggcagccct acaaacccga aggagtagaa atgaaactgt
    10021 ccgaactgtt caaccccaac gaatttgccg cgcggcattt gagtttcggc gacgaagcgg
    10081 cgttgcttgc cgctgtcggc gagaaaagta tggacgattt tgtcggcaac accgtgccgc
    10141 aaagcatccg tatgccgtcc gaactcgatt tgcccgaagc cctgaccgaa gcggacgcat
    10201 tggcgaaatt gaaaggcatt gcgtcgaaaa acatgatcaa caaatcctat atcggtttag
    10261 gctattaccc gacccgcgtg ccgaacgtga ttttgcgtaa cgtattggaa aatccgggtt
    10321 ggtacaccgc ctacacgccg tatcaggcgg aaatcgccca ggggcgtttg gaagcgttgt
    10381 tgaacttcca acaagtgtgt atcgatttga ccggcttccc cgtggcaggc gcgtctttgc
    10441 tggacgaagc gactgccgcc gccgaagcga tggcgatggc gcaccgcgtg ggcaaagtga
    10501 aatccgagcg tttcttcgtg gatgcgcgcg tgtatccgca aactttggac gtgatgaaaa
    10561 cccgcgccaa atatttcggc tttgaattgg tggtcggcga ttttgctcaa gccgacgagg
    10621 gcgaatactt cggcgcgctg ttccaatacg tcggcaaaga cggcgatgtg caagacttgc
    10681 aggacgttat cggccgtctg aaagcaaaag gcacaatcgt tgccgtttcc gccgacatta
    10741 tgagcttggt tttgctgaag tctccggctg aattgggtgc agatattgct ttgggcaaca
    10801 cgcaacgctt cggcgtgccg atgggcttcg gcggtccgca tgctgcttat ttcgcgttta
    10861 aagacgagtt caaacgttcc gccccgggcc gtatcatcgg tgtatccaaa gacgcatcgg
    10921 gcaaacctgc cttgcgtatg gcgttgtcca cccgcgagca acacatccgc cgcgaaaaag
    10981 ccacgtccaa tatttgtacc gcgcaggcat tgctggcgaa tttggccggt atgtatgccg
    11041 tttaccacgg ccctgaaggc gtgaaacgca tcgccaaccg cattcacgcg ctggcttctg
    11101 cctttgccga tgcactggtt tcagacggcc tgaatgtggt tcataaggtc ttcttcgata
    11161 ccgttaccgt cgatttcggc aacaaagaga aagcagacca agtgtttgcc gctgctttgg
    11221 aatcgggcta taacctgcgc cgcgttaacg atactcaagt tgcggctgca ttccacgaaa
    11281 catcggcatg cgaagatttg gttgatttgt accgcgcgtt taccggcaag gatgcgttcg
    11341 catttgccga tgatgtcaaa ggccgtctga acgccgaatt gctgcgtcaa gatgacattc
    11401 tgcaacatcc tgtgttcaac cgttaccaca ccgaacacga aatgttgcgc tatctgaaaa
    11461 aactcgaaga ccgcgatttg gcgatgaacc gcagtatgat ttcattgggc agctgtacta
    11521 tgaaactcaa cgcgactgcg gaaatgttgc cgattacttg ggcggagttc tccgacatcc
    11581 acccctacgc ccccgaaacc caaaccgcag gctaccgcga actcttaaca gacatggaaa
    11641 acagcctgaa agccatcacc ggctttgacg cgatttcctt gcaacccaac tccggcgcac
    11701 aaggtgaata caccggtatg ctctccatcc gccgttatca agaagccaac ggcgagggac
    11761 accgcaacat ctgtttgatt cccaaatccg cccacggtac caaccctgcg actgcggcca
    11821 tgctgggctt gaaagtcgtc gttgtcgata ccgacgaaca cggcaacgtc aacatcgacg
    11881 atttgaaagc caaagccgag caacaccgcg acactttgtc cgccatcatg attacctatc
    11941 cgtctaccca cggcgtgtac gaagaaggca tccgcgacat ctgccgcatc atccatgaaa
    12001 acggcggaca ggtttacatg gacggcgcca acctcaacgc ccaaatcggc atcatgcagc
    12061 ctgccgaagt cggcgcggac gtgttgcaca tgaacctgca caaaaccttc tgtatccctc
    12121 acggcggcgg cggtccgggc atgggtccga ttggcttgaa agcccatctg gctccgttcg
    12181 caccgggaca cgctttgacc gacacccaca gcgccagtgc cgaccaaacc gccgttgctg
    12241 ctgcggcctt cggttctgcg tccatactgc cgattacttg gatgtatctg accatgatgg
    12301 gcaaacaagg catggaacag gcgacacgct gggcattgct caacgccaac tacgttgcca
    12361 aacgcctgag cgaagactat ccgattctgt atacaggcaa aaacggccgc gtcgcgcacg
    12421 aatgtatcgt cgatttgcgt ccgctcaaag ccgaaagcgg cattaccgaa accgacatcg
    12481 ccaaacgcct gatggactac ggcttccacg ccccgacggt ttccttcccc gttgccggta
    12541 cgctgatgat cgagccgacc gagagcgaaa gcaaagccga actcgaccgc ttcatcgccg
    12601 ccctgaaaca aatcaaacag gaagtgctga aagtcgggcg cggcgaatgg ccgaaagacg
    12661 acaacccact ggtcaacgcg ccgcacaccg ccgcagatat aaccggcgac tgggcgcgcc
    12721 cgtattcccg cgaagaagcc gtcttcccgc tgcccttcgt gcgcgaacac aagttttggc
    12781 ccttcgtcaa ccgcgtggac gacgtgtacg gcgaccgcaa tctcgtgtgc agctgcccac
    12841 cgatggaaaa ttatgaagac tgaccgttga aaccccgaag acaaatgccg tctgaaacac
    12901 tttcagacgg cattttcgtc gacggcagac tggttgcacc gatacacgta tctcgactat
    12961 aactttaaaa caaatgggtt aaaccagtat ccatacatca gcttttttat catcctactt
    13021 tttattcatc cgatcgggca gacagatttc aaagatgaaa agcctattca caccctttga
    13081 tgtcatttcc acacggacaa acaaatatag tggattaaca aaaaccagta cagcgttgcc
    13141 tctccttagc tcaaagagaa cgattctcta aggtgctcaa gcaccaagtg aatcggttcc
    13201 gtactatctg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
    13261 aattcccata aaaaaacgga gcagatacct gccccgtttt tatttaatcc gaaattttaa
    13321 tctaaattta gaattttgca ccggattggt ttgccatata gtcaacagcc gctttgactt
    13381 cgtcatcgct caaacctgca ttgccgcctt tggccggcat cgcgttaaag ccttcaaggg
    13441 cgtgtttgtg caaggtttct ttgccttttt tgatacgcgg tgcccaatcg tcttttttgc
    13501 ctatgccggg aataccggga atcgaaccgc cgtggcacac ctgacaggtt gcttcgaaga
    13561 cttttttacc gtcaacgccg accgcagggg ctgccgcacc cttgtcttct gccttcgctt
    13621 ctgccggagc tgcactatcg gcaggagcag aagctgttcc tgaagcggca ttgtcggcag
    13681 gcgcagcctc atcaggattc gggaaagaac cgccgctttt gttcgccatg taagtaatcg
    13741 cccgtttgag ttcctgatcg gtcaggtctg ccgcaccgcc tttggcaggc atggcgttaa
    13801 agccgttcag cgcgtgttgg aacaaggtat cgaagccttg cgcgatacgc ggtgcccaat
    13861 cgccgttgtg ttccagtttc ggagcgttcg gcacattgct gtccgccgcg tggcattgga
    13921 tacagatttt gccgaaaatc tgttcgcctt ggcgttcgcc gacggggatg ccgtcgccca
    13981 tcgtcaattg tccgacgggc tggatacggg tctgcgttgc cgcttccgta gtggcatcga
    14041 catcgccgaa cgagccgctg cccgccagct taatcaggaa ataaaggact gcaataacaa
    14101 taacgatacc gctcacaagg gtaaacagtg cagagccttg ggctttgttg tcgcggagtt
    14161 gtttcatttg gtaggcctcg ccgtcaggtt aggttgtgct gtaaattata gtttggtgtg
    14221 ttaaacgcag ttaacaatat tttgctggat tatactgaat tcacagggtc tttccaatcg
    14281 ctatcattga aaatatgaaa aaatttgcca acggtatctg tataaaacaa ataatccttt
    14341 taaaataatg gtttatcctc aagaaaactc tccttatgcc gccatacgcc gcctgccggc
    14401 gcaagataac ctttgccaat ttgcagaatt tacgttaacc ttacgtttta cgcacccata
    14461 gctcagttgg aagagtgtca gtttccgaag ctggaggtca caggttcgat ccctgttggg
    14521 tgcgccaatt ataaagagac cgtctgaaag ataaatattt ttcagacggt cttttgactt
    14581 acttcaaact cttatttcaa gacttccgca aatgcgcggg caacatagtc ggtattcgac
    14641 gtattcagtc cggcgacgca catcctgccg gaatccagca ggtaaacggc aaattcgtcg
    14701 cgcagcctgc ggacttgttc cacgctcaat cctgtgtagc cgaacatgcc gcgctgtttg
    14761 atgaaataag tgaaatcgcg atcggggatt tgcgcagtta agacatcata aagtttctgc
    14821 cgcatcgcac ggatgcggtc gcgcatcata taaacctcgt tttgccacaa ggcgtaaagt
    14881 tcggggctgt tcatcacgtc ggcggcgata tacgcgccgt gcgcgggcgg gctggagtag
    14941 atgcggcgga cagtgaattt gagctgtccg aacaccaaat ccgcttcttc cttattcggg
    15001 caaaccacgc tcaggccgcc gacgcgctcg ccgtagagcg acaggttttt tgagaaggaa
    15061 ttgctgacga acaagggcaa ttccatttcc accgctttgc ggacggcgta ggcatcgctg
    15121 tccaaatcgc cgccgaagcc ttggtaggca atgtccataa acggaatcag tttgcgcgtt
    15181 ttgatgatgt gcaacacttc gtcccattgc tgttccgaca tatccacgcc ggtcgggttg
    15241 tggcagcagg gatggaggat caggacgctg ttttcgggca gggtgttgaa aaacgcggtc
    15301 atttcgtcga atttcacgcc aacagtggca gggtcgtaat atgggtaagt gccgacctcg
    15361 aaacctgcgc cttcaaaaat gccgcgatgg ttgtcccaag tcgggtcgct gacgtaggcg
    15421 cgcgcttcgg gaaaccagcg gtgcaggaag tccgccccga ctttgagtgc gcccgagccg
    15481 cccaaggttt gtacggtaac gatgcgccct tgcgcgagcg cagggttgtc tgtgccgaac
    15541 aataaatgct gcaccgcgct gcggtaagtg tccaagcctt ccatcggcag gtagggcgac
    15601 ggcgcaggcg tggcggcgcg ggcggtttcg gcacggcgca cggattccaa cacgggcatt
    15661 ttgccttcgt cgtcgaaata aatgcctatg ctcaaattga ctttttcagg gcgcgggtcg
    15721 tttttgaagg tttcgaccaa actcaaaatc gggtcgcccg gatagtattc gatgtgtcgg
    15781 tacatagtcc ttacctttta ctttttcaaa ggattttctt tttcaacaat acaccacttt
    15841 cgatatggtg cgtaaacggg aattggtcga acagggcggc gcgttcgacc gcatgggttt
    15901 ccaccaaggt gtccaaattg gcacgcagcg tttcgggatt gcaggaaatg tagatgatgt
    15961 tgtcaaactg cgacaccagc ttcaaagttt cctcatcgat accggcacgc ggcggatcga
    16021 cgaaaatagt ggaaaatgcg taatccgtca aagcaatacc gccatcctta aggcgtttaa
    16081 actcacgttt tctggtatag gcttcggtaa attcttcagc agacagacgg gcgattttga
    16141 tgttgccgat gcggttggct tcgatattcc attgcgccgc gctgatggag gttttggaga
    16201 tttcggttgc caaaacacgt tcaaactttt cagataaagg aagcgtaaag ttgccgtttc
    16261 cgcaatacag ttcgagcaaa tccccatcca atccttctgc tgcaccgcag gcccattcaa
    16321 gcattttctg acacacggcg gcattcggtt gggtaaaact gccttcaatt tgccgataac
    16381 gaaaatcccg attaccgacc tttaaagttt ccgttacata gtcctgtttt aagactattt
    16441 tctgtcccct gctccgccca ataacggaaa tatccaactg ttgctgtaac gcttgcgccg
    16501 cctgcatcca ctcattatca agccttttgt ggtaaatcat ggtaaccagc atttccccgc
    16561 tgagcgtgga cagaaattcg acggcatacc agcgtttttt gagttcgggg gattgcgcgg
    16621 cggcggcgat cagctcgggc atgaggcggt tgacagcctc ggaagctgct tcaaaacggt
    16681 cgcagcgtat catgcttgcg ccgctggctt tctgcccttt ttcaaacatg gcataaaaca
    16741 tttccccgcc ttcgtgccaa atacggaact cggcacgcat acggtagtgt ttgtccggag
    16801 attcgtacac ctcccactcg ggaacatcca aacctgcaaa aagggtttta aggcggtctt
    16861 ttttatcttg aagctgctgc gtgtagtcgc tcatatcgct atcctgaaaa atcagaccgg
    16921 attataagcc gattttcctc gccgcacgca aactctttga ctgccgcccc ttcaatgacg
    16981 gacgggattt tgtgctaaaa tccgccatct ttccacacta tactgataaa gggaaaaatc
    17041 atggcaggca acactttcgg acaactcttc accgtgacca cgttcggcga aagccacggc
    17101 gcaggtttgg gctgtatcat cgacggctgc ccgcccggac tggcattaac cgaatcagac
    17161 atccaagccg acctcgaccg gcgcaaaccc ggcaccagcc gccacgttac ccaacgccgc
    17221 gaagccgacc aagtcgaaat cctctccggt gtcttcgaag gcaaaaccac cggcacgccc
    17281 atcgccctct taatccgcaa taccgaccaa cgcagcaaag actatggcaa catcgcccaa
    17341 agtttccgcc ccggccacgc cgactatacc tactggcaca aatacggcac gcgcgactac
    17401 cggggcggcg gtaggagttc cgcccgcgaa accgccgccc gcgttgctgc cggagccgtt
    17461 gccaaaaaat ggttgaaaga aaaattcggc acggaaatca ccgcctacgt tacccaagtc
    17521 ggcgaaaaaa aaatccggtt tgaaggctgc gaacacattt cccaaaatcc tttttttgcc
    17581 gccaaccaaa gccaaattgc cgagctggaa aactatatgg acagtgtgcg caaatcgttg
    17641 gattccgtcg gagcgaagct gcatattgaa gccgccaacg tccccgtcgg cttgggcgaa
    17701 cccgtattcg accgcctcga tgccgaaatc gcctacgcga tgatgggcat caacgccgtc
    17761 aaaggcgtgg aaatcggcgc aggttttggc agcgttgtcc aacgcggcag cgaacacggc
    17821 gacgaactga ccccgcaagg cttcctgtcc aaccactcag gcggcatcct cggcggcatc
    17881 agcaccggac aagacatcca cgtcaatatc gccatcaaac ccaccagctc cattgccaca
    17941 ccgcgccgca gtatcgacat caacggcgac cccgtcgaac tcgccaccca cggcaggcac
    18001 gacccctgcg tcggactgcg cgccgccccc attgccgaag ccatgctcgc gttagtcctc
    18061 atcgaccacg ccctgcgcca tcgcgcgcaa aatgcggatg ttgccgtgga tacgcccgat
    18121 atcgcccgct cggacaagta aaaagattta gccaaaacac gggctttata ctaaaatacc
    18181 gaccattgcc gtgccgtctg aacgcacgat tcttagagga atacaaccga aatgacacaa
    18241 gaaaccgctt tgggtgcggc actgaaatcc gccgtccaaa ctatgagcaa aaagaaacag
    18301 accgaaatga tcgccgacca catctacggc aaatacgatg tgttcaaacg cttcaaaccg
    18361 ttggcactcg gcatcgatca ggatttgatt gccgctttgc cgcaatacga ttccgcactg
    18421 attgcacgcg tcctcgccaa ccactgccga cgtccgcgct atctgaaagc cttggcgcgc
    18481 ggcggcaaac gtttcgattt gaacaaccgt ttcaaaggcg aagttacccc cgaagaacag
    18541 gcgatcgcgc aaaaccatcc tttcgtgcag caggctttgc aacagcagtc cgcccaagct
    18601 gtcgccgaaa cgccgtctgt tgaagccgaa gcagccgaat cttccgcagc agaataaatc
    18661 cccaaacgaa atgccgtctg aaaaccgatt tggtttcaga cggcattttt tcgtatgcgg
    18721 caatcacggt tcaaatatcg agttccgccg tatcgccttc gcgttccatc caggcacggc
    18781 gggcggcggc ttcgcctttg cccatcagtt tgacgaagat gtcgcgcgtc tcgtcatccg
    18841 cgccttcggg gatttgcacc tgcaacaggc ggcgggtgtc agggtgcatg gtggtgtctt
    18901 tgagctggtc ggggttcatt tcgcccaagc ccttgaaacg gctgatggaa taggcggttt
    18961 ctttgacgcc ttctttttgc agccgctcca aaatgctgtc gagttcgttt tggtcgaggg
    19021 cgtagaattt gcgggcaggt ttgcttttgc cttgtgcgtt gacatcgacg cggaacagcg
    19081 gcggctgggc gacgtagatg tgtccgtcgg caaccagttt cgggaagtgg cggtagaata
    19141 aggtcagcag caaaacttga atatgcgagc cgtccacgtc ggcatcggac aggatggcga
    19201 ttttgccgta gcgcaggccg cttaaatcgg gatggtcgtt aataccgtgc ggatcgacgc
    19261 cgatggcgac ggaaatgtcg tggatttcgg cgttgccgaa gagttggtcg gggtggactt
    19321 caaagctgtt gagcactttg ccgcgcaggg gcaggatggc ttgggtggct ttgtcgcggg
    19381 cgagtttggc ggagccgccg gcggaatcgc cttcgacaag gaagagttcg ttttcgcgga
    19441 tgtcttcgct ttcgcagtcg gtcagcttac cgggcaggac ggcgacgccg ctgccttttt
    19501 tcttttcaat ctttttaacc gaacgcatcc gcgcctgtgc ctgacggata gcgagttcgg
    19561 cgattttttt gccgaagtcc acattttggt ttagccacaa ttccaaagga tcgcccgata
    19621 cagtggcaac gagtttcagc gcatcgcggt tggtcagctt gtctttggtt tgaccttgga
    19681 actgagggtc gaggacacgg gcggagagga cgaaggcggt tttaccgaac acgtcgtcgc
    19741 tttgcacttt cacgccgcgc ggcaagaggt tgtgcaggtt gatgaagttg ttgacggcgt
    19801 tgaacacggc ttgtttcaag cccgcttcat gcgtgccgcc caaaggggtg ggaatcaggt
    19861 tgacgtagct ttcgttggca cacgagcctt cttccagcca agtcagggca aaggcggcgc
    19921 cttcgccgat gctgaaatcg ccgttgtgtt cgtctgaaat gtagttttcg caagagaaca
    19981 gcggtacggc ttcctgcgcg tcggcaatca ggtcggtcag atagcttttc aggccgtcgg
    20041 ggtaatgcca ggtttgggtg tgcgcttcgt cttcgccttt gaccggacgg gtcagggaaa
    20101 cgcgcacacc cggcagcagc acggctttgg cacgcagcag gcgttcgagt tcgagaatgc
    20161 tgtaattcgg gctttcaaaa tatttgccgt ccggccacac gcgcacttcg gtgccgccgt
    20221 ctttgacggc gcatttgccc acttgtgcca acggttcgac cacgtcgccg ccggcaaaca
    20281 cgatacggtg gattttgcct tcgcgtttaa ccgttacttc aaggcgggtg gaaagggcgt
    20341 tggtcacgga tacgcccacg ccgtgcaggc cgcctgaaaa ggcatacgcg ctgccgccgt
    20401 cttttttgtt gaacttgccg cctgcgtgca gacgggtgaa cacgagttcg actacgggta
    20461 cgccttcttc agggtgcaga ccgacgggaa taccgcggcc gttgtcgcgt acggaaagcg
    20521 aaccgtcttc atgaatttgc acgtcgattg ccgtagcgaa accgcccaac gcctcgtccg
    20581 ccgcgttatc aatcacttcc tgacagatat gggtcgggct gtcggtgcgg gtgtacatgc
    20641 cgggacgttc tttgaccggc tccaagcctt tgaggacggt gatgctggat tcgctgtatt
    20701 ggttgttttt agccatagga ataatctgaa agtaagaaaa acaacgcttt cagacggcct
    20761 gaaagcgttg cgttccgttg ttttaccggt tgccggaagg ttggcgggcg gcaaagtctt
    20821 cataactttc cataccgcgc aggaagcggg aagagagttc tttcaacgcc cccaaataaa
    20881 cgtcgcgttt gaactcgatg acttcgtcaa cgggcgccca atattgatgc caacgccagc
    20941 cgtcaaattc ggggtggcgg gtggcgcgca ggttgacatc gcaatcccgg ccggtcaggc
    21001 gcaggagata ccaaatctgc ttctgcccgc gataagagcc gcgccattcg cggcgcaccc
    21061 agttgttcgg cacgtcgtaa cgcagccagt cgcgcgtccg cccgacgatt ttgacgtgtt
    21121 gcggcagaag tccgacttct tcgtaaagct cgcggtacat cgcggtttcg gggctttcgc
    21181 ccggcttgat gccgccttgc ggaaactgcc aagaatgttc gcgcacgcgc ttaccccaaa
    21241 agacttcgtc gcggttgttg gttaagatga taccgacatt ggggcgatag ccttctctgt
    21301 ccaacacggt gtcgccctcc gttaaattca atcttgggat tttcccacaa atcgaacggt
    21361 tttgacaaat cagacggtat ggttcaagca tgtcgaaaca cggacggatt cgggaaaata
    21421 tcttaaattt ggtttacaat aatgtgtttc aaattaattc gggaatcaga ccatgttaga
    21481 tatccaattg ctccgcagca acaccgccgc cgttgccgaa cggcttgcac ggcgcggtta
    21541 tgactttgat accgcacgtt ttgacgcact ggaagaacga cgcaagtccg ttcaggtgaa
    21601 aactgaagaa ctacaagcct cgcgtaacag catttctaaa caaatcggcg cactgaaagg
    21661 tcagggcaaa cacgaagagg cgcaggcggc catggatcaa gtcgcccaaa tcaaaaccga
    21721 tttggaacag gctgccgccg atttggatgc cgttcaaaaa gaattggacg catggttgtt
    21781 gagcatacct aacctgccgc acgaaagcgt acctgccggt aaagacgaaa ccgaaaacgt
    21841 cgaagtccgc aaagtcggca ccccgcgcga atttgacttt gaaatcaaag accatgtcga
    21901 tttgggcgaa cctttgggtt tagattttga aggcggtgca aaactctccg gcgcacgatt
    21961 taccgtgatg cgcggacaaa tcgcccgtct gcaccgcgcc ttggcacagt tcatgctgga
    22021 tacgcacacg ctgcaacacg gctacaccga gcattacaca ccctatatcg tggacgacac
    22081 caccttgcaa ggcacgggtc agctgccgaa gtttgcagaa gatttgttcc acgttacccg
    22141 tggcggcgac gagagcaaaa ccacgcaata tctgattccg acagctgaag ttaccctgac
    22201 caataccgtt gccggcagca ttatcccgtc cgaacaactg ccgctgaagc tgaccgcgca
    22261 ttcgccctgt ttccgcagcg aggcgggttc gtacggcaaa gacacgcgcg gtctgattcg
    22321 ccagcaccag ttcgacaaag tggaaatggt tcaaatcgtt catcccgaaa aatcatacga
    22381 aacgctggaa gaaatggtcg gccatgccga aaacatcctg aaggctttgg aactgcccta
    22441 ccgcgtgatt accctgtgta ccggcgacat gggcttcggc gcaaccaaaa cgtatgattt
    22501 ggaagtttgg gtgccggcgc aaaataccta ccgcgaaatc tcaagctgct ccaactgcga
    22561 agatttccaa gcccgccgca tgaaggcgcg tttcaaagac gaaaacggca aaaaccgctt
    22621 ggtacatact ttgaacggct ccggcttggc tgtcggcaga acgctggtcg ccgtattgga
    22681 aaaccatcaa aacgccgacg gcagcatcaa catccctgcc gcactgcaac cgtatatggg
    22741 cggcgttgcc aagttggaag tgaaataagt ttgcaagctg cctgaacgtc aaatgccgtc
    22801 tgaaacctgg ttcagacagc atttccttta aacttttaaa acacgtcagc cgtcggcacg
    22861 aaccgcattg ccgcaatcgc cggtctgtcc gacctcgcgg atattggaca gcgtaacttc
    22921 cgaaatatta cccaacgcct cttccgtcaa aaacgcctga tgtccggtaa acagcacatt
    22981 atgacaagac gacaggcggc ggaacacgtc gtcggtaatc acatcgttgg atttgtcttc
    23041 aaaaaacaat tcgcgttcgt tctcgtacac gtccataccc aatgcgccga ttttccggcg
    23101 tttcaacgcc tcaatcgcgg cagaactgtc aatcagaccg ccacggctgg tgttgataat
    23161 catcacaccg tctttcattt tgtcgaacgc cgcttcgttc agcatatagt ggttttccgg
    23221 cgtggcgggg caatgcagcg tgatgatgtc cgactgggca tacagctcgt ccaaatccac
    23281 atacctgccg ccgatttttt ccgcttcggg gttgcaaaac ggatcgtgag ccagcaggtt
    23341 catgccgaaa ccctttaaaa tccgcatggt tgcaataccg attttccccg tgccgataac
    23401 gcccgccgtt ttgccgtaca tattgaaacc ggtcagacct tccagcgaaa aattcgcatc
    23461 gcgggtacgc tgataggctt tgtggatacg gcggttcaaa gtcagcatca gaccgaccgt
    23521 atgttccgca accgattcgg gcgaataggc aggcacgcgc acgactttca agcccaactc
    23581 ttcagccgcc tttaaatcca cattattgaa gccggcacaa cgcaacgcca cagttttcac
    23641 gccaatttgc gccaattttt ccaacacggg ccggctgccg tcgtcgttta caaaaataca
    23701 aaccgcttcc gcgccttccg ccattttcgc cgttttcgca tccagcataa aatcaaaaaa
    23761 ctccagctcg aagccgaaat gccggttggc gcgggtaaaa tgttcgcggt cataactttt
    23821 cgtaccgtaa atcgcaatct tcatcaatat gtccagttgt cgcctatggt tgagaaacgg
    23881 cataatacac tgaattcaaa caaatcagta gaatatggcg aattaaaatt gattgcatgc
    23941 acggcatttc catttcaaaa cacaaaactc aatcgcccat cgccgccaga agttctgcct
    24001 gatgttcggc aatcagggca ttggtgattt ctgccaaatc gccgtccatc acgaaatcca
    24061 gcttgtgcag ggtaaggttg atgcggtggt ctgttaccct gccttgggga tagttgtagg
    24121 tgcggatgcg ttcgctgcgg tcgccgctgc cgatgaggga tttgcgttcg gcggcttctt
    24181 tggcttgggc ttcgcgtttt tgcgcgtcgt tcaggcgggc ggcgaggact ttcatcgcct
    24241 gcgctttgtt ggcgtgttgg ctgcgtccgt cttggcattc gaccaccatg cctgtgggca
    24301 ggtgggtgat gcggacggcg gaatcggttt tattgatgtg ctgcccgccc gcgccggatg
    24361 cgcggaaggt gtcgatgcga aggtcgacgg ggttcaactc gatgtcttcc agttcgtccg
    24421 cttcgggcat gacggcaacg gtgcaggcgg aagtatggat gcggccttgg ctttcggtgg
    24481 cggggacgcg ctgcacgcgg tgtccgcccg attcaaattt caaacggctg tacgccccga
    24541 gtccgacaat acgggcgatg acttctttat agccgcccaa ctcgctttcg ttggcggaaa
    24601 cgatttcaac ctgccagcgg ttgcgttcgg cgtagcggct gtacatacgc agcaaatcgc
    24661 cggcaaacag cgcggcttcg tcgccgcccg ttccggcgcg tatttcgatg aagatgtttt
    24721 tgtcgtcgtc ggcatctttg ggcagcagca gtttttgcag ttcggtatcg agcgtgttga
    24781 ttttggcttt tgccgcttcg atttcttcgg cggcaaagtc tttcatttcg gggtcggaca
    24841 gcatttcttc ggcatctgcc aagtcgcttt gcgccagttg atagttttgg aatacttcga
    24901 cgacgggggt cagttcggcg tgttcgcgcg tgagcttgcg gtagttgtcc atatcggatg
    24961 tggcttcggg ttgccctaaa aggtgggtaa cttcttccaa ccggtcgccg agttgttgca
    25021 gtttttctaa gatagacggc ttcataattc ttccataaca aacgccgcct gaatttcaga
    25081 cggcatcaaa ccgcggtcat tataatatat tttctgaata ttcaaaaaga taatctaggg
    25141 acggagcagg ctgtcggcat tgcgttccgt tatcgcggcg atttccgccg cgccggtacc
    25201 gcgtatctgc gcggcaattt cggcaatccg aagaatgttg gcaggcgtat tgacggtgtt
    25261 tttcaacata aacgggctat ccgtttccaa tacgaaatcc ccgtcgttca aggctttaag
    25321 cgtatcgcgc actttacgcg cgttcgggtt gagcagcagc gaaccgatgc cgattttgaa
    25381 acccagtttc gccaacacgt gcgcttcttc aatactgccg gaaaaggcgt ggacaatgcc
    25441 gccccaggta aagcccgtcc gtttgacggc ggcggcgatg tcggcggcgg ctttgagatt
    25501 atggataatc acgcgccgac gcagggtttg cgcgatttca agctggcggc tgaagacttg
    25561 gatttgccgt tcgcgctgct gcggcgtttg ggttttgtcg taaaaatcca agccgatttc
    25621 gccgacccat gcctgcggat aacgtgccaa catcgcttcc aggcggacgc aatcacgctc
    25681 ggcaatgccg tctgaaaacc aaggatggat gccgagcgca atacggattt gaccgtgttc
    25741 ggacggcatt tccgacagtt ccgccacgct ttgccaatcc tgcggacagg tggcgggaac
    25801 gataaaccgc ttcaccccaa ctttccgcgc ggcggtcagg atgtgcggca ggttttcgcg
    25861 cagggcggca tcggcaaggt ggcagtgggt gtcggtgaag ttcatttcga ttccgcaatg
    25921 cggcaggttc acataaaaat aaggcagggc ggcaagggat gccgtgtacg ggcggcgcat
    25981 aaggcgcggt caacgccccg tcattgccat ttcaatccgc tataacttca gtcttgccaa
    26041 ctctttttct acatcttccg cgccccagcc ttgcgatacg gcgagggtca tcagcgcggt
    26101 ggcggtttcg aggttgcatt tgccgccgtt gatgatgccc gagttgcgga acgcattgcc
    26161 ttgcgcgtaa acggcggcag ttctgccttg tcggacttgg ctgatgttga gcaacaattt
    26221 tccttgccgc gtgaagtctc ggacagcgcg gataaagcct tcgtctgcgg gcgtgttgcc
    26281 gtgtccgtaa ctttgcagga taagggcgtg ggcgggaagc tgtccgagtc cgtcggcaag
    26341 ttcttggacg gcaaagccgg ggataagcgt gcggacggcg atttttgcct gcgggtcggg
    26401 ataacggatt ttgaggccgt ctgaaacatc tgccgtgtcc tgattcggga tgcggacatt
    26461 gtgccagccc tgggtttcgt cccactccgc cagtgcgccg aaatgcggat tgtcgaaacc
    26521 ggcggcggtt tcggtgctga ctttgctgct gccgacggcg ggataaagtt tgccgtcaaa
    26581 ggcaataagc gtctgcttca atccgagtgg gaatgcggca acggcagtcg ccagattgcg
    26641 cggcgcgtcg ctgcccacgg cgttgtacgg ccattgggag ccggtcagga cgattggctt
    26701 gtccaaaccc tgcagcgcga gggcgaacag attggcggta taggcgagcg tgtccgtgcc
    26761 gtgcagcacc aaaacgccgt cgtactgcgg cagcgcggcg gcaatgatgt ccagccaagc
    26821 gcaccagttt tgcagttgga cggcggaaga atcaatcagc gtcccacaaa cgtgccaatc
    26881 gaaatccatg ccgtttgaaa acggcgcgag tgcctgcccg accagcgcgg tatcggggcg
    26941 caggccttcg ctgctttgcg tcatgccgat ggtgccgccg gtataaagga caaaaatttt
    27001 ctgtttcatg gctcatcctg aaaatgcaaa gaccgcctga aacgcgaaat gcgtccggac
    27061 ggtcgaaaat ctgatttact gctgcttctg tgccgctttt ttcgctgcct tttccgcctt
    27121 gcgtttggcg cgtttttcgc tcaattgtgc gcggtaaagc tgataatgtc ggcgtttgcg
    27181 ccaccagaac cacgccagcg ccgccgccag cacgcccaat gcgatgaaga tgccggattg
    27241 caggctgtgc attttcgcca tcagccaatc gatgttgtgc gcgccgtact cgcccaagta
    27301 aatccaaacg ggcacggaaa tcagcgcggc aagcccgtcc ataatcagaa agcgcagata
    27361 cgatactttg cggctgatgc cggcggtaac gaaaacggca gtccgcaaac ccggcaggaa
    27421 acgagcgaca aacagaaccc agttgccgta tttgtcgaat ttttcctgaa cctgtgcgta
    27481 acgtttcggc gtcatgatgc gcgcaatcgg tttgaacttg aggattttct gcccccagat
    27541 gcgtccggcg gcgaacatga tgccgtcgcc cgccaacacg ccgagcatac cgactgcaaa
    27601 cataatatgc ggattggtat aacccatacc cgaaatcacg ccgcctgtta ccaaggtcaa
    27661 atcctcgggg atcggcacgc cgaaaccgca gatgaccaaa acgaaaaaca cggccgcata
    27721 gccgtattcg acaaaaaagg cttccaaaag ggcaaacata gcggatattc cattgtcgga
    27781 gataaaaagt cagaacaagc cgaaacattt tctgcgcgaa acggcattct atcaaagatt
    27841 atgccgtctg aaagcggcaa ggggcagatt cgcgccctct gccggacgtt cagacggcat
    27901 ttaaacagga aaaccgccgc tgttgaaacg gttttgcctg attttgccta aaggctgccg
    27961 atggcggaag caatacgttc cgcaccgtcg cgcgcccaat ccgcctgccg cgcctccacc
    28021 atcacacgca cgacgggttc ggttcccgaa gcgcgcaaca cgacacgccc tttgccttcg
    28081 agttcttttt ccacttccgc caacacgtct tttgaggctt cctgccattt ctgacctttt
    28141 tggatgcgca cgttaatcat agtttgcgga tacggctgcc aatcggcgca aacggtggcg
    28201 aggtcttggt tcagcgtttg cagtgccgcc aaaacttgca gcgcggaaat aatgccgtcg
    28261 ccggtgttgt gtttgtccat gcacaaaata tggccgctgg cttcgccgcc gatgagccag
    28321 ccgcgttggt tcagctgttc caacacatag cggtcgccga ctttggcgcg gcagaaatcc
    28381 acgccctgct ctttcagggc gatttccatc gccatattgg tcatgaccgt gccgaccacg
    28441 ccgccgatgt tgataccttc tcgggcgcgg gctttagcaa tgacgtaaat caggctgtcg
    28501 ccgtcgtaaa ccttaccgtt tttatcgacc atcatcaggc ggtctccgtc gccgtctaag
    28561 gcgatgccgt agtcggcttc atgctgtaaa acggctgcct ggagtgtctt ggtataagtc
    28621 gcaccgcatt tttcattgat attgtagccg ttgggttcgt cgccgatgct gacgacttgt
    28681 gcgcccagtt cgtgaaacac cttgggtgcg acaccgtacc ccgcgccgtt ggcagtatcg
    28741 ataaccaact tcaaaccccg aaggtcggaa tggctgggaa aggtagattt gcaaaattcg
    28801 atatagcggt cgtccgcacc actgatgcgg cgtgcgcgac cgaggcgtgc agaaggctgg
    28861 gttttcattt cttcgtcgat tttggcctcg atttccaact cgatttcatc gctcagtttc
    28921 acgccgccct cggcaaagaa tttgatgccg ttgtcggaat aggcgttgtg cgacgcggaa
    28981 atcatcacgc cggcggacag gcgcaacgcg cgggtcagat aagccacgcc gggcgtgggc
    29041 agcggtccgg tctgtaccac attcacaccc gccgccgtaa aaccggccac caaagcggct
    29101 tccagcatat agccggaaat acgcgtatcc ttgccgatta aaacggtcgg tttctggtcg
    29161 gtgtcgtgct gaaccaaaac ctgacccgcc gcatagccga gtttcaatac aaaatcgggc
    29221 gtaatcggaa actgcccgac ttcgccgcgc acgccgtccg tgccgaaata tttttttgcc
    29281 atgtgttgct ccgagaatgt gaaccgttgt ccgagattat acagtcagtt tgtgccttgc
    29341 tgtctgcacc gttgatgccg tctgaagccg ccccgtcctt ttcagacggc atgaagtatg
    29401 cgaaccgctg tttacagatt gatgcccaac gcttcccaca ccttcaacgc gtccgccgtc
    29461 gccttcacgt catgcacccg cacaatttgc gcgccgcgcg ccacagccgc cacagccgcc
    29521 gccacgctgc cgtgtacgcg cgccgccgcg tcggtttcgc ccgtcagttc gcctaccatg
    29581 cgtttgcgcg atacgccgat cagcagcgga aagcctgttt ccgccatcaa ttcgggcaaa
    29641 tgcctcatca gcgcgatatt gtgttgcagg gttttgccga agccgaaacc cggatcgagc
    29701 gtgatgcgtt gcggtgcgat gcctgccgcg atgcattccg ccgcccgcgc tttcaaatac
    29761 cgcgccactt caccgacaac atcctgatat ttcggattaa tctgcatgtt ttcaggcaaa
    29821 ccccgcatgt gcatcaggca aatgcccgtg tccgtctggc gcgccagcaa ttcgaccgca
    29881 ccttcgtcgg tcaacgccgc cacatcgtta ataatatcga tgccaccgag tgccaacgct
    29941 ttttccataa tcaccgtgcg gcgcgtgtcc aaactgacgg gaacgcccca ccccgccact
    30001 tctgccaata caggctcaac ccgcgcccat tcttcttcgg acgaaacata atccgcaccc
    30061 ggccgcgtcg attcgccgcc gatgtcgaga atgtccgcac cctctttcaa aagccgttcg
    30121 gcgtgcgcca aagctgttcg ggcgttttgc gaatacgcgc cgccgtcgga aaaagaatcg
    30181 ggcgtgagat tcacgatgcc catgattttc ggcttgtcca aaccgatttc aaaccgtcct
    30241 gcctgccaaa cgtgtcgtgc catctgaact cctcccaaaa taaaaaaac
//
LOCUS       DAXHTY010000016        29315 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXHTY010000016 DAXHTY010000000
VERSION     DAXHTY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29315
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.016"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            182..538
                     /locus_tag="ACFNMK_000592"
     CDS             182..538
                     /locus_tag="ACFNMK_000592"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC6363916.1"
                     /translation="MLSAYFPVFVFILIGLAAGVLFILLGTILGSKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKELGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            529..1011
                     /locus_tag="ACFNMK_000593"
     CDS             529..1011
                     /locus_tag="ACFNMK_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC6363917.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1024..1617
                     /locus_tag="ACFNMK_000594"
     CDS             1024..1617
                     /locus_tag="ACFNMK_000594"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC6363918.1"
                     /translation="MASIQNLYETVVGVLGDQASKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            1607..2863
                     /gene="nuoD"
                     /locus_tag="ACFNMK_000595"
     CDS             1607..2863
                     /gene="nuoD"
                     /locus_tag="ACFNMK_000595"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC6363919.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2863..3336
                     /gene="nuoE"
                     /locus_tag="ACFNMK_000596"
     CDS             2863..3336
                     /gene="nuoE"
                     /locus_tag="ACFNMK_000596"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC6363920.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPVQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            3726..5027
                     /gene="nuoF"
                     /locus_tag="ACFNMK_000597"
     CDS             3726..5027
                     /gene="nuoF"
                     /locus_tag="ACFNMK_000597"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC6363921.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVSFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            5027..5464
                     /locus_tag="ACFNMK_000598"
     CDS             5027..5464
                     /locus_tag="ACFNMK_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363922.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            5754..8015
                     /gene="nuoG"
                     /locus_tag="ACFNMK_000599"
     CDS             5754..8015
                     /gene="nuoG"
                     /locus_tag="ACFNMK_000599"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC6363923.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIIQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVHSAIKCIAKDGNQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLASSKEELFMPLLSQEAVHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQIAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8018..9094
                     /gene="nuoH"
                     /locus_tag="ACFNMK_000600"
     CDS             8018..9094
                     /gene="nuoH"
                     /locus_tag="ACFNMK_000600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC6363924.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            9175..9654
                     /gene="nuoI"
                     /locus_tag="ACFNMK_000601"
     CDS             9175..9654
                     /gene="nuoI"
                     /locus_tag="ACFNMK_000601"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC6363925.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            9685..10392
                     /locus_tag="ACFNMK_000602"
     CDS             9685..10392
                     /locus_tag="ACFNMK_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363926.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            10428..11099
                     /locus_tag="ACFNMK_000603"
     CDS             10428..11099
                     /locus_tag="ACFNMK_000603"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC6363927.1"
                     /translation="MTFQLILFYIFAVIILYGAIKTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            11096..11401
                     /gene="nuoK"
                     /locus_tag="ACFNMK_000604"
     CDS             11096..11401
                     /gene="nuoK"
                     /locus_tag="ACFNMK_000604"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC6363928.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            11435..12010
                     /gene="fic"
                     /locus_tag="ACFNMK_000605"
     CDS             11435..12010
                     /gene="fic"
                     /locus_tag="ACFNMK_000605"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6363929.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            12134..12475
                     /locus_tag="ACFNMK_000606"
     CDS             12134..12475
                     /locus_tag="ACFNMK_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363930.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            12524..14548
                     /gene="nuoL"
                     /locus_tag="ACFNMK_000607"
     CDS             12524..14548
                     /gene="nuoL"
                     /locus_tag="ACFNMK_000607"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC6363931.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLILFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYVVI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQIFRPIYVLFENKYYLDVLYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSARLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            14763..15083
                     /locus_tag="ACFNMK_000608"
     CDS             14763..15083
                     /locus_tag="ACFNMK_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HFC6363932.1"
                     /translation="MSNTQTICSADFTASRAWGALDIANMNGTTVRLHWTNQPYKWHV
                     NDGEEVFAVMDGEVDMHYRENGEEHIARLRSGDIFYAGIGAEHVAHPRGETRILVIEK
                     EGSV"
     gene            15158..16873
                     /locus_tag="ACFNMK_000609"
     CDS             15158..16873
                     /locus_tag="ACFNMK_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFC6363933.1"
                     /translation="MANLNVSSKKIINLLGNQDMQEKYFIIPEYQRPYRWDTEKCAVL
                     WSDIVDFRQSSTGKNEEYFLGTIVVCPNEKGNLEVIDGQQRLTSLLLLLRAFYRVLEQ
                     TESTPDTAAKITTLKSKIAPCIWDVDVLTAEITDRTAIRLHTQVATETDNEILHEILA
                     NGEISEECRSNYAVNYLYFLKQCQEYARTNPMDWYHLCLTILNDCILLPIESDSLNSA
                     LTIFSTLNDRGLPLSDADIFKARLYKMAQQKDKFVEQWKELVALAEGASISVDDLFRY
                     YTHVLRAKEKITDKEIGLRRFYTDNRNKQLENENLFNHLIELAGFWQDINNSKTNQDV
                     ETSNFVNDEAAKWLQCLNVYPNEFWKYAASVFFIANKGAQSFKDDFAALLKQLTAFLY
                     AKFLVAPTVTAIKDDIYKLCVAIYHGEKNALSYVMPAPIHYEQNTGRITKGLLMINAY
                     LFDEKQKLLPTKTEIEHIFPKKWQETNYNGWSIIDAEQYLEQLGNKIVFEKRLNIQAG
                     NGYFGKKKEQYQKSDIVEVRELAGENANDWLPSDIQKRQQQIVDRLKQFFDNCFEAAS
                     IPKKN"
     gene            16910..18406
                     /locus_tag="ACFNMK_000610"
     CDS             16910..18406
                     /locus_tag="ACFNMK_000610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC6363934.1"
                     /translation="MFSNHLLSLAIWIPIAAGVLVLATGSDSRALLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIYGAIHNPHVAEM
                     KDINCREFAILAILAVVVLGMGLYPQAFIEVVHQAANDLIAHVAQSKI"
     gene            18416..19861
                     /gene="nuoN"
                     /locus_tag="ACFNMK_000611"
     CDS             18416..19861
                     /gene="nuoN"
                     /locus_tag="ACFNMK_000611"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC6363935.1"
                     /translation="MNWSDLNLMPAMPEVALLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKVAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            19973..20272
                     /locus_tag="ACFNMK_000612"
     CDS             19973..20272
                     /locus_tag="ACFNMK_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC6363936.1"
                     /translation="MTASMYILLLLALIFANAPFLTTKLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(20363..21259)
                     /locus_tag="ACFNMK_000613"
     CDS             complement(20363..21259)
                     /locus_tag="ACFNMK_000613"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC6363937.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAV
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGGKALRLRQLAEFAVARKY"
     gene            complement(21243..21551)
                     /locus_tag="ACFNMK_000614"
     CDS             complement(21243..21551)
                     /locus_tag="ACFNMK_000614"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC6363938.1"
                     /translation="MPSEILLFQTASGFQKCYNSVFTETPETMKKNAPKSFEEALSRL
                     ESLTQSMQGEMPLEDALAAYQEGNELVRYCQTKLAQVEQKLQVLDADGLKELNLESDE
                     "
     gene            complement(21579..22502)
                     /gene="rsgA"
                     /locus_tag="ACFNMK_000615"
     CDS             complement(21579..22502)
                     /gene="rsgA"
                     /locus_tag="ACFNMK_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC6363939.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENADLLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGKIRTERLTFLQRITDDLLK"
     gene            complement(22507..24363)
                     /locus_tag="ACFNMK_000616"
     CDS             complement(22507..24363)
                     /locus_tag="ACFNMK_000616"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6363940.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAY
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLNVPQGAIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAVGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(24389..24526)
                     /locus_tag="ACFNMK_000617"
     CDS             complement(24389..24526)
                     /locus_tag="ACFNMK_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363941.1"
                     /translation="MPPEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(24526..25110)
                     /gene="ruvA"
                     /locus_tag="ACFNMK_000618"
     CDS             complement(24526..25110)
                     /gene="ruvA"
                     /locus_tag="ACFNMK_000618"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC6363942.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPMGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(25192..25518)
                     /locus_tag="ACFNMK_000619"
     CDS             complement(25192..25518)
                     /locus_tag="ACFNMK_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6363943.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQYSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(25572..26291)
                     /locus_tag="ACFNMK_000620"
     CDS             complement(25572..26291)
                     /locus_tag="ACFNMK_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC6363944.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASASSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            complement(26288..26410)
                     /locus_tag="ACFNMK_000621"
     CDS             complement(26288..26410)
                     /locus_tag="ACFNMK_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6363945.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(26421..26885)
                     /gene="trmL"
                     /locus_tag="ACFNMK_000622"
     CDS             complement(26421..26885)
                     /gene="trmL"
                     /locus_tag="ACFNMK_000622"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC6363946.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(26950..27675)
                     /locus_tag="ACFNMK_000623"
     CDS             complement(26950..27675)
                     /locus_tag="ACFNMK_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC6363947.1"
                     /translation="MDFLSRWRRIADAPTIRRCVLCHGSSGVSDGICAGCRDDLAAYR
                     TDAANSCPLCFMHVQGGSVCGGCQKKPPAFDRMWASLHYEPPVSNMIHALKHLADLSM
                     ARPLANLMMQNPPDRLSDECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQIL
                     PRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            27674..28417
                     /gene="bioH"
                     /locus_tag="ACFNMK_000624"
     CDS             27674..28417
                     /gene="bioH"
                     /locus_tag="ACFNMK_000624"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC6363948.1"
                     /translation="MPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTAAEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLAR
                     CGTPQALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRRLKGSRL
                     VVMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            28419..29204
                     /locus_tag="ACFNMK_000625"
     CDS             28419..29204
                     /locus_tag="ACFNMK_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6363949.1"
                     /translation="MNHQDARWQVHRHLAEHTDQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAARKGGFWQRFAGKGVVQHCQSPTAPLPEACADM
                     LWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSVM
                     FPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAAR
                     SCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
BASE COUNT         6691 a         7194 c         8012 g         7418 t
ORIGIN      
        1 cgaattacgg tgtaaaaatt tatatgacct tataaaatca aataagaatc gttatcataa
       61 catgattgta tttattgggt ttttttgggc gttttgccga tatttacctt ttaatggttt
      121 ttgaaattcg ctaaaatacg aaattattgt agaaattttg ttaacggatt tgggtgtaac
      181 catgttgtcc gcttactttc ccgtctttgt ctttatcctc atcggcctcg cggccggcgt
      241 actgtttatc ctgctcggca caattttggg ctcgaaacgt cactatgccg aaaaagacgc
      301 accttacgaa tgcggttttg aagcctttga aaacgccagg atgaagttcg acgtgcgcta
      361 ttacctcgtc gccatcctct ttatcctgtt tgatttggag gtcgcgttta tgctgccgtg
      421 ggcggtggtg tttaaagaat tgggcgcgta cggcttctgg tctatgctgg tgtttatcgt
      481 cgttctgacg gtaggctttg tttacgaatg gaaaaaaggt gcgctggaat gggaatagaa
      541 ggcgttttga aaaaaggttt catcaccacc agcgcggata cggtgttgaa ctatatgcgt
      601 accggttcat tgtggccggt tactttcggc ttggcctgct gcgccgtgga aatgatgcac
      661 gcgggtatgg cgcgttatga ccttgaccgc ttcggcatca ttttccgtcc gtccccacgt
      721 caggccgacc tgatgattgt ggcaggtacg ctgaccaata aaatggcgcc cgccctgcgc
      781 cgcgtatacg accagcttgc cgagccgcgc tgggtattgt ctatgggctc gtgtgccaac
      841 ggcggcggtt attatcacta ttcttattcc gttgtgcgcg gtgccgaccg cgtcgtgccg
      901 gtagatgttt atgtgccggg ttgtccgccg actgcggaag ccctgattta cggcctgatt
      961 cagctccaac aaaaaatcaa gcgcacttcc accatcgcgc gtgacgagta aggagaggac
     1021 gatatggcaa gcattcaaaa cttatacgag accgtcgtcg gtgtgcttgg cgatcaggca
     1081 agcaaagtca tttcagcttt gggcgagatt accgtcgagt gtctgcccga acactatatt
     1141 tccgtcatga ccgcattgcg cgaccatgaa gaactgcatt ttgagcttct ggttgacttg
     1201 tgcggtgtcg attacagcac ttacaaaaac gaagtatggc agggcaaacg cttcgccgtc
     1261 gtcagccagc ttctttccgt taaaaacaat cagcgcatcc gcgtacgcgt ctgggtttca
     1321 gacgacgact tccccgtggt cgaatccgtc gtcgatattt acaacagcgc ggattggtac
     1381 gaacgcgaag ccttcgatat gtacggcatt atgtttaaca accacccgga cttgcgccgc
     1441 atcctgaccg attacggttt cgttggacat ccgttccgca aagacttccc gatttccggc
     1501 tatgtggaaa tgcgttacga cgaagagcaa aaacgcgtga tttaccaacc tgttaccatc
     1561 gagccgcgcg agatcacgcc gcgtatcgtc cgtgaggaga actacggtgg ccaataaatt
     1621 aagaaactac accatcaact tcggcccgca acaccctgcg gcgcacggcg tattgcgtat
     1681 gattttggag ttggacggcg aacaaatcgt ccgtgccgac ccgcatatcg gcctcctgca
     1741 ccgcggtact gaaaaactgg ctgaaaccaa aacctatctg caagccctgc cttatatgga
     1801 caggctggac tacgtttcca tgatggtcaa cgagcaggca tactgcttgg cggtagaaaa
     1861 actcgccgga attgatgtac ctatccgcgc ccaatacatc cgcgtgatgt tcgccgaagt
     1921 aacgcgcatc ctcaatcact tgatgggcat cggttcgcac gcattcgaca ttggtgcgat
     1981 gaccgccatc ctttatgcct tccgcgaccg cgaagagttg atggacttgt acgaagccgt
     2041 gtccggcgca cgtatgcacg cagcctactt ccgtcccggc ggcgtttacc gcgacctgcc
     2101 cgacttcatg cccaaatacg agggcagcaa attccgcaat gccaaagtat tgaagcagct
     2161 caacgaatcc cgcgaaggca cgatgctcga ctttatcgat gccttctgcg aacgtttccc
     2221 gaaaaacatc gacacactcg aaaccctcct gaccgacaac cgtatttgga aacagcgtac
     2281 cgtcggcatc ggcgtcgtct cccccgaacg tgccatgcaa aaaggcttta ccggcgtgat
     2341 gttgcgcggt tcgggcgtgg aatgggacgt gcgtaagaca cagccttacg aagtgtacga
     2401 caaaatggat ttcgacatcc ctgtcggcgt gaacggcgac tgctacgacc gctacctctg
     2461 ccgtatggaa gaaatgcgtc aatccgtacg catcatcaaa caatgttccg agtggttgcg
     2521 tgtcaatccg ggtccggtca ttaccacaaa ccacaaattc gctccgccca aacgtaccga
     2581 aatgaaaaca ggtatggaag acctgattca ccatttcaaa ctctttaccg agggtatgca
     2641 cgttcccgag ggcgagacct acaccgctgt cgaacatccg aaaggcgagt tcggcgttta
     2701 catcatttca gacggcgcaa acaaacccta ccgcctgaaa atccgcgcac ccggcttcgc
     2761 ccatctgcaa ggcatggacg aaatggcaaa aggccacatg ctcgccgacg tcgttgccat
     2821 catcggtacg caggacatcg tattcgggga ggttgaccga taatgttatc cgcagagtcc
     2881 ttaaaacaaa tcgacacaga actggcgaaa tatcccgccg accaacgccg ttccgccatc
     2941 atgggtgcgt tgcgtatcgc ccaaaccgaa aaaggctggc tcgcccccga aaccatcgct
     3001 tttgttgccg actatatcgg catcacgcct gtacaagcct acgaagtcgc cactttctac
     3061 aatatgtacg accttgagcc tgtcggcaaa tacaaactga ccgtttgtac caacctgccc
     3121 tgcgccctgc gcggcggtat ggctaccggc gaatacctca aacaaaaact cggtatcggc
     3181 tacggcgaaa ctacccctga cggcaagttt acccttgtcg aaggcgaatg catgggcgca
     3241 tgcggcgacg ctcccgttat gctggtcaac aaccacagca tgtgcagctt tatgaccgaa
     3301 gaagcgattg agaagaaact ggcggagttg gagtaggtcg tctgaaacga cgatttaaac
     3361 gtaggtcgga tacttgtagc cgacagagtg ggtaaaaagg caaaatgtcg gatttaagaa
     3421 tccgccctac tgaaataccg aaatgccgtc attcccgcgc aggcgggaat ccaccggtaa
     3481 gattcggttt ctgaatttaa taagacattg cttaccattg aggatggatt cccgcctgcg
     3541 cgggaatgac gacagacaag caagtggtcg agatccaaca aaaacgatta aaggtcgtct
     3601 gaaaatatcg atttgataaa ctagatttta tttcagacga cgttacaagc cggtacacaa
     3661 agacatctta aggtcgtctg aaacagcggc cgcaaccgat acgaaaacaa acaggcacac
     3721 caaaaatggc tatttaccaa tcaggcgtga tttttgacca agtggatacc gccaatcccg
     3781 attgctggac attggacgaa tacgtcaaac gtggcggtta taccgccctg cgtaaaatcc
     3841 tgtccgaaaa catctcgcaa accgatgtga ttgacgaagt caaaacctcc ggcttgcgcg
     3901 gtcgtggcgg tgcgggcttc ccgaccggtt tgaaatggag ctttatgccc cgttctttcc
     3961 ccggcgaaaa gtacgtcgtt tgcaacaccg acgaaggcga gccgggtaca tttaaagacc
     4021 gcgacattat tatgttcaac ccccatgccc tgattgaagg catgattatc gccggttacg
     4081 cgatgggcgc gaaagccggt tacaactata tccacggcga aatttttgaa ggctaccaac
     4141 gctttgaggc cgctttggag caggcgcgtg ccgcaggctt tttgggtaaa aatattttgg
     4201 gttcggattt tgaattcgaa ctcttcgccc accacggcta cggtgcatac atctgcggcg
     4261 aagaaaccgc attgcttgaa tcgctggaag gcaaaaaagg ccagccgcgc tttaaaccgc
     4321 cattccctgc ttcgttcggc ctgtacggca aaccgactac catcaacaat accgaaactt
     4381 tctcctccgt ttcattcatt atccgtgacg gcggacaggc atttgccgat aaaggtattc
     4441 cgaatgcagg cggtaccaaa ttattctgta tttccggcca tgtcgagcgt ccgggcaact
     4501 atgaagtgcc attgggcaca ccgtttgccg aagtcttgaa aatggcaggc ggtatgcgcg
     4561 gcggcaaaaa actcaaggcc gttattcctg gcggttcgtc cgcgcccgta ttgcctgccg
     4621 acatcatgat gcagacaaat atggactacg actcgatttc caaagcaggc tcgatgctgg
     4681 gttctggcgc gattatcgtc atggacgaag acgtgtgcat ggtgaaagcc cttgagcgcc
     4741 tgagctactt ctactacgac gagtcttgcg gccaatgtac gccttgccga gaaggcaccg
     4801 gctggcttta ccgcatcgtc caccgcatcg tagaaggcaa aggccgcatg gaagatttgg
     4861 atttgctgga ttctgtcggc aaccaaatgg caggccgcac catctgcgcc cttgccgatg
     4921 ccgccgtctt ccccgtccga agctttacca agcatttccg tgatgagttt gtgcattaca
     4981 tcgaacacgg cggaccgatg aaagagcata agtggggagg gtggtaatgg tggaagctaa
     5041 aatttttatt ctatacggtg cagccaacaa aggtaagagt acgacactca atacgctttt
     5101 taatcagatt tgttggaaat tttctaaatt tctagtcttt tttgaaagac atggaaatgg
     5161 cttagatttt gttgcagtat ttgatcatga aggtcagaga attggttttt attcatctgg
     5221 tgataatgaa tacgaggtta ggagaaattt atacaaactt tattcgcata attgtgattt
     5281 tatttttggc acgtcaagga cacggggtgg tagttgcgat gcagtaggat gttatgcaga
     5341 gttattgcat ggcgatgtaa atataattaa ttggtgtgaa aagtttgagc ctacagatga
     5401 agacaatgag cgtgctgtta aagagttatt taagtcattt aaaaatataa taaatgagtt
     5461 atagttttag ttggttttat attggttaaa agcaaaatgc taaaaattta actttgccgt
     5521 cattcccgcg taggcgggaa tccatagtgg aatttacaga acccgatatt tgaaaagcag
     5581 ttgccgaaat tcaaaaaatg gattcccgcc tgtgcgggaa tgacggcaag gtggcagtaa
     5641 acgttttaaa cagtattgat tgtcaatgaa actcaaaagg ccgtctgaaa cccatttttc
     5701 agacgacctc cataaaagat tatttatcaa atacccgtaa ctaggaacga accatgttac
     5761 aaatcgaaat cgacggcaaa caagtatctg tggagcaggg cgcgacggtg attgaagccg
     5821 cgcacaagct cggtacttat attccgcatt tctgttacca caaaaaactt tccatcgccg
     5881 ccaactgccg tatgtgtctg gtgaacgtag aaaaagcccc aaaacccctg cctgcctgtg
     5941 ccacgccggt tacagacggc atgattgtgc gtacgcattc ggcaaaagcc cgagaggcgc
     6001 aggaaggcgt gatggagttc ctgctcatca accatccgct tgattgtccg acctgcgacc
     6061 aaggcgggga atgccagttg caggatttgg cggtgggcta cggaaaaacc accagccgct
     6121 acaccgaaga aaaacgttcc gtcgtcggca aagacatggg gcctttggtt tccgccgagg
     6181 aaatgagccg ctgtatccac tgcacccgct gcgttcgttt cactgaagaa atcgccggtt
     6241 tgcaggaaat tgcgatggtg aatcgcggcg aacactccga aatcatgccc tttatcggca
     6301 aaacggtgga aaccgagctg tcgggcaacg tcatcgattt gtgtcctgtc ggcgcattga
     6361 ccagcaaacc gttccgcttc aacgcgcgta cttgggaatt gaaccgccgc aaatccgttt
     6421 ccgcccatga cgctttgggc agcaacctga ttatacaaac caaagaccat accgtccgcc
     6481 gcgtgttgcc gctggaaaac gaagcgatta acgaatgttg gctgtctgac cgcgaccgtt
     6541 ttgcctatga aggcctgtat cacgaaagcc gtttgaaaaa cccgaaaatc aaacagggcg
     6601 gcgagtggat ggacgtggat tggaaaaccg cgttggaata tgtccacagc gcaattaaat
     6661 gtatcgccaa agacggcaac caaaatcaag tcggcgtttg ggccaatccg atgaatacgg
     6721 ttgaagagct gtatttggcc aaaaagcttg ccgacggctt gggtgttaaa aactttgcca
     6781 cccgtctgcg ccaacaagac aaacgtcttt cagacggcat taaaggtgcg caatggttgg
     6841 gacaaagcat tgaatcgctg gcggacaacg atgccgtatt ggtagtcggt gcgaacctgc
     6901 gcaaagaaca gccgcttctg accgcccgcc tgcgccgcgc cgctaaagac cgcatggctt
     6961 tgagcgtatt ggcaagcagt aaagaagaat tgtttatgcc gcttctgtct caagaagccg
     7021 tacatcccga cgagtgggca ggtcgtctga aaaacctgtc tgccgatgcg gaacacgccg
     7081 ttaccgccag cctgaaaaac gctgaaaaag cagcggtgat tttgggcgcg gaagtgcaaa
     7141 accatcctga ttacgccgcc atttatgccg ccgcgcaaga gctggcggac gcgaccggcg
     7201 cagtgctggg tattttgccg caagccgcca acagtgttgg tgcggatgtc ttgaatgtaa
     7261 actccggcga gagcgttgcc gaaatggcaa acgcgccgaa acaggcagtc ttgttgctca
     7321 acgtcgagcc ggaaattgat acggttgacg gtgcaaaagc cgtagccgcg ttgaaacagg
     7381 caaaaagcgt gatggcgttt acgccgtttg tcagcgaaac gctgctggac gtgtgcgacg
     7441 tgttgttgcc gattgcaccg tttaccgaaa cctcaggcag cttcatcaat atggaaggcc
     7501 gtctgcaatc tttccacggc gtggtacaag gcttcggcga ttcgcgtccg atgtggaaag
     7561 tattgcgcgt attgggcaat ctgtttgacc tgaaaggttt tgaatatcac gataccgctg
     7621 cgattctgaa agacgcgctg gatgcagaaa gcctgccgtc caaactggac aaccgtagca
     7681 catggacagg ggagggcgtt cagacggcct cagaccgcct tgtccgtgtc ggcggcgtcg
     7741 gtatttatca cactgattct atcgtgcgcc gttccgcacc gttgcaagaa accagccatg
     7801 ccgccgtgcc tgccgcgcgt gtaaatccga acactttggc acgcttgggc ctgcaagacg
     7861 gacaaattgc tgtcgccaaa caaaacggcg caagcgtatc ggttgccgtc aaagccgatg
     7921 ccggtttgcc tgaaaacgtg gtgcatctgc cgctgcatac cgaaaatgcc gcgctgggtg
     7981 cgttgatgga cactattgaa ctggcgggag cttgatcatg caagaatggt tccaaaacct
     8041 ctttgccgca acgctcggtc tgggcgattt gggtattacc gtaggcttgg tggtatccgt
     8101 catcgtcaaa attgtgatta tcctgattcc gctgattctg accgttgcct acctgacgta
     8161 tttcgaacgt aaagtcatcg gctttatgca gcttcgtgtc ggcccgaacg taaccggccc
     8221 gtggggtctg attcagccgt ttgccgacgt gttcaaactc ttgtttaaag aagtaacccg
     8281 tccgaagttg tcaaacaaag ccctgttcta catcggcccg attatgtcgc tcgccccgtc
     8341 ttttgcggca tgggcagtga ttccgttcaa cgaagaatgg gtgctgacca atatcaatat
     8401 cggccttttg tacatcctga tgattacctc gctgtcggtt tacggcgtga tcatcgcggg
     8461 ctgggcttcc aactccaaat attcgttcct gggcgcaatg cgtgcctccg cgcaaagcat
     8521 ttcttacgaa atcgccatga gtgccgcgct ggtgtgcgtc gtgatggtat cgggcagtat
     8581 gaacttctcc gacatcgttg ccgcgcaagc caaaggtatt gccggcggtt cggtattctc
     8641 gtggaactgg ctgccgctct tccccatctt catcgtttat ctgatttccg ccgttgccga
     8701 aaccaaccgt gcgccgtttg acgtggcaga gggtgagtct gaaatcgttg ccggtcacca
     8761 cgtcgaatat tccggcttcg cgttcgcgct gttcttcctt gccgaataca ttttcatgat
     8821 tctgattgcc gcgctgacat cgttgatgtt cctcggtggc tggttgtctc cgttcccgca
     8881 aagctggggc tttatcggta cgccttccgc attctggatg ttcgtgaaaa tggcggcggt
     8941 tctgtactgg tacctgtgga tccgtgcaac cttcccgcgc taccgttacg accaaatcat
     9001 gcgtttgggc tggaaagtgc tgattccgat cggcttcgcc tacatcgtgg ttttgggcgt
     9061 gtggatgatt tcaccgctga atttgtggaa ataagtttca gacggcatct tgaggccgtc
     9121 tgaacaaagc gattttgaat acctaacgaa atccctgttt tgagggaaca taatatggct
     9181 aacttagtaa aaacctttct gcttggcgaa ttggtaaaag gtatgggcgt aacgctcaaa
     9241 aactttttcg cccgcaaaga cacgatttat tttcccgaag agaaaacgcc gcaatccgtg
     9301 cgtttccgcg gcctgcacgc gcaacgccgt tatccgaacg gcgaagagcg ctgtattgcg
     9361 tgtaaattgt gtgaggcagt ttgtccggca atggcgatta acatcgaatc ggaagaacgt
     9421 gaagacggta cccgccgcac caagcgttac gacatcgatc tgaccaagtg catcttctgc
     9481 ggtttctgcg aagaggcctg cccgaccgat gcgattgtgg aaacccatat ttttgaatac
     9541 cacggcgaga aaaaaggcga cttgcacatg accaagccga ttcttttggc cattggcgat
     9601 aaatacgaag ctgaaatagc caaacgcaaa gccgctgacg cgccgtatcg ttaatgcttt
     9661 ggggcttctt ggaaggtttt aaatatggaa ggactgatta atgcattgaa atatttagcc
     9721 gaacatgagc caatagataa ttttgaagaa attagaacta gaaatagtcc gattgagttg
     9781 ccaagtggat taagtaattt tgaacaaaat atttttttaa aagaaaattt atccccaaaa
     9841 ttacaaaatg atgatagctt gaagacgcat tattggatta tccgtgaatg gggtgggatt
     9901 aaaagtttta aacaatctgc tgaaaatagc cagcttattc gtcaattttt atcggaactt
     9961 aattcgggaa aattgagtag tggtttgttg aaaatttcat cattatctaa attggcttct
    10021 tttatagatt gtgagcgatt cgccatttat gattcacgcg ctattttttc gttgaattgg
    10081 ttgttgttta aatttacaaa tgcagatttg ttttttcagc cacaaggtag aaatagggaa
    10141 ctagaaatcc gaaatatgaa cgtattgttt catttttctg atatcaaacc gaattatcgg
    10201 aaaccagacg tttcgtttca tcaatattgt ggattgttac aagatttggc gaaacaagtt
    10261 tatggtaaac aagcaaaacc gtatcacata gaaatgttgt tattcaaaat tgcgacaacg
    10321 tggatttgtg cggatatgga tcaactgatt aagtttgatt gtttgcgtaa ccaggatttt
    10381 cagactgctt gaaaccatat ttttgattaa taaagaaagc atagactatg actttccaac
    10441 tgattttatt ttatattttt gcagtgataa ttctttatgg cgcgatcaaa accgtcaccg
    10501 ctaaaaatcc tgttcacgcc gctttgcatc tggtgttgac cttctgcgtg agcgcgatgc
    10561 tttggatgct gatgcaggct gagtttttgg gcgtgacgct ggtggtggtt tacgtcggcg
    10621 ccgtgatggt gttgttcctg ttcgtcgtga tgatgttgaa catcgacatt gaagaaatgc
    10681 gtgccggttt ctggcggcac gcgcctgttg ccggtgtggt cggcacattg ttggcagttg
    10741 ccctgattct gattctggtc aatccgaaaa ccgaccttgc cgcatttggg ctgatgaaag
    10801 acattcccgc cgattacaac aacatccgcg atttgggcag ccgtatttat accgactatc
    10861 tgctgccgtt tgaattggcg gcggtattgc tgctgttggg catggtagcc gcgattgcgc
    10921 tggttcaccg taaaaccacc aatccgaaac gcatggatcc tgccgaccaa gttaaagtac
    10981 gtgccgacca aggccgtatg cgtctggtga aaatggaagc ggtcaaaccg caagtcgaat
    11041 ctgccgaaga aagcgaagtt tcagacggtc tcaagccgga aggggagggc aaagcatgat
    11101 taccttgacg cattatctgg tattgggcgc gctcctgttc ggtatcagcg cgatgggcat
    11161 ctttatgaac cgcaaaaacg tgctggtctt gctgatgtcc atcgagctga tgcttttggc
    11221 ggtgaacttc aactttatcg ccttctcgca acatttgggc gataccgccg gacagatttt
    11281 cgtattcttc gtactgaccg ttgccgctgc cgaatctgcc atcggtttgg cgattatggt
    11341 attggtgtac cgcaaccgac aaacaatcaa cgttgccgat ttggatgagt tgaaagggta
    11401 aaggtaggtt gggtcgaaac ctgacaagac accgatgccg tctgaaaacc cgataggaaa
    11461 aacgatgaaa tccatagacg aacaaagcct gcataatgcc cgccgcctgt ttgaaagcgg
    11521 cgacatcgac cgtatcgaag tcggtaccac cgcgggcctg caacagattc accgttacct
    11581 gttcggcggc ttatatgatt ttgcgggtca aatcagggaa gacaacattt ccaaaggcgg
    11641 ttttcgtttt gccaacgcca tgtatttaaa agaggctttg gttaaaatcg agcagatgcc
    11701 cgagcggact tttgaagaaa tcatcgccaa atatgttgaa atgaacattg cccatccgtt
    11761 tttggagggt aatggcagaa gtacccgcat ctggctggat ttggtgctga aaaaaaacct
    11821 gaaaaaagtc gtgaactggc aaaatgtaag taaaaccctg tatttgcagg cgatggaacg
    11881 cagccccgtc aacgatttag aactgcgctt tctgttaaag gacaacctga ctgacgatgt
    11941 ggacaaccgt gaaatcatct ttaaaggtat cgagcagtcg tattattacg aagggtatga
    12001 aaaaggctga gggtcgtctg aaaagcgatt tcagactgtt tcagacgacc tgattcggta
    12061 ggtgatcaga cgggagcgga tgagaaaaga aattctgggt aagaataatc cggtctgaaa
    12121 tattggaaga agaatgatgg ataaaaatca gttagaacaa gaatttcata aagccatgtt
    12181 aaatatttat caggaggctt tgaatttgcc gcaaccttac aaggcgacac gatttttaca
    12241 aattgtaaat gaatttggtg gtaaagaggc ggcggataaa ttattgagta cgggggaaaa
    12301 gaagactcag accggtttta cagagctgat tttgagtggt ggcggagtcc acgccttgaa
    12361 atacagtatg gaatatctgg tgttacaaaa gccgtggtgt gatttattta ctgaagagca
    12421 attagctgtg gcacgcaaac gattggagcg tgttggattt gtttttccga agtaattttg
    12481 tacgaaacaa acatagattt ttaaatcaat cggattcaat caaatgaacg acatgacttt
    12541 atatctcatc atcgccctcg tgcctttggc aggctcgctg attgcgggtt tgttcggcaa
    12601 caaaatcgga cgtgccggcg cgcatacggt tacgatactc ggtgtggcgg tgtctgccgt
    12661 gctgtcggct tatgtgctgt ggggattcct caatggcagc cgtgccaagt ttgacgaaaa
    12721 cgtctatacc tggctgacga tgggcggttt ggatttctcc gtcggcttct tggttgatac
    12781 gatgacggcg atgatgatgg tcgtggtaac gggcgtgtcg ttgatggtgc atatctacac
    12841 catcggctat atgcacgacg aaaaagtcgg ctaccaacgc ttcttcagct acatttcttt
    12901 gtttacattc agcatgttga tgctgattat gagcaacaac ttcatccagc tcttcttcgg
    12961 ttgggaagcg gtgggtttgg tgtcgtatct cttgatcggt ttctatttca aacgcccgag
    13021 cgcgacattt gccaacctga aagccttttt gatcaaccgt gtcggcgact tcggcttttt
    13081 gctcggtatc ggcttggtgc ttgcctattt cggcggcagc ctgcgctatc aggacgtatt
    13141 tgcctatctg cccaacgtcc aaaatgccac tatccagctt ttccccggtg tggaatggtc
    13201 tttgattacc gtaacctgcc tgatcctgtt tgtgggcgcg atgggtaaat cggcacaatt
    13261 cccactgcac gtttggctgc ctgattcgat ggaaggcccg accccgattt ctgcattgat
    13321 tcacgccgca accatggtta ccgccggttt gttcatggta tcgcgtatgt cgccgattta
    13381 tgagatgagc agcactgcgc tgtctgtcat catggtaatc ggtgcgatta ctgccctgtt
    13441 tatgggcttc ttgggcgtga ttcaaaacga catcaaacgt gtggttgcgt attccaccct
    13501 gtcgcaattg ggctatatga ccgtggcttt gggcgcgtcc gcctattccg tggcgatgtt
    13561 ccatgtgatg acccacgctt tcttcaaagc cttgttgttc ttggcggcag gcagcgcgat
    13621 tatcggtatg caccatgacc aagacatgcg ccatatgggc aacctgaaaa aatatatgcc
    13681 gattacttgg ctgaccatgc tgatcggtaa cttgtcgctg attggtacgc cgttcttctc
    13741 cggcttctat tccaaagatt caattatcga agcggcgaaa tacagcaccc ttcccggcag
    13801 cggctttgct tattttgccg tcctcgccag cgtgttcgtt accgcgtttt acgcattccg
    13861 ccaatacttt atggtgttcc acggcgaaga aaaatggcgc agcctgcctg aacatcatga
    13921 cgatcatcat ggcgaagagc atcacggctt gggtaaaaac gacaatccgc acgaaagccc
    13981 gttggttgtt accctgcctt tgattttgct tgccattccg tccgtcatca tcggctacgt
    14041 tgttatcgag cctatgctct acggcgattt tttcaaggat gtgattttcg tcaacgccga
    14101 cgcgcatccg accatgcaca tcatgaagga agagttccac ggcgcattgg caatggtatc
    14161 gcacagcctg cattcgcctg tactctacct cgctatcgca ggtgtattga gcgcatggct
    14221 cctgtacgtc aaactgccgc acctgcctgc gaaaattgca cagatattcc gtccgattta
    14281 tgttttgttt gaaaacaaat actacctcga cgtcctgtat ttcaacgttt ttgccaaagg
    14341 cacgcgcgca ttgggtacct tcttctggaa agtcggcgat accgccatta tcgacaacgg
    14401 tattgtcaac ggctctgcca gactggtcgg cgcgattgcc gcgcaagtgc gtaaagccca
    14461 aaccggcttt atctacacct acgccgccgc tatggtattc ggcgtattgg tcttgctcgg
    14521 catgaccttc tggggcttgt tcagataaag gcgggtttca gacgacctgt tgatagtttt
    14581 gtagaggtcg tctgaaaaga accattcaat ttgtaggtcg gatacttgta tccgacatga
    14641 acaagaaaac agtacagcgt gtcggattcg agaatccgac taaggcatat cgggattgtt
    14701 cagataaaaa tgagattttc agacgacgtt taggtcgtct gaaaaaagaa acttgagaaa
    14761 caatgtcaaa cacacaaacc atctgctctg ccgacttcac cgcttcccgc gcatggggcg
    14821 cactcgacat cgccaatatg aacggcacca ctgtgcgcct gcactggacg aaccagccct
    14881 acaaatggca cgtcaacgac ggcgaggaag tgtttgccgt gatggacggc gaagtggaca
    14941 tgcactaccg tgaaaacggc gaagagcaca tagcacggct gcgaagcggc gacatctttt
    15001 atgccggcat cggcgcggaa cacgtcgccc acccgcgcgg cgaaacgcgg attttagtga
    15061 ttgagaaaga aggcagtgtt tagggcggat acgcataagt attatgtgct ttttcaggta
    15121 tcctgaaaat cataaatcta tcaacgagac tttaatcatg gcaaatctaa atgtgagtag
    15181 taaaaaaatt attaatctgc taggtaatca agatatgcag gaaaaatatt ttatcattcc
    15241 tgaatatcaa cgcccctatc gctgggatac cgaaaaatgc gccgtattat ggtctgatat
    15301 tgtggatttt cgccagtcgt ccacaggaaa aaatgaagaa tattttcttg gtacaatcgt
    15361 tgtttgcccc aatgaaaaag gtaatttgga agtaattgat gggcaacagc gtttaacatc
    15421 attactgctg ttattgcgtg cgttttatcg agttttagag caaacggaaa gtacccccga
    15481 taccgcagcc aaaattacca cacttaaaag caaaattgcc ccctgtattt gggatgttga
    15541 tgttctaacg gctgaaatta ccgatagaac cgcaatccgg ctgcatacgc aagttgccac
    15601 agaaacagat aacgaaattt tgcacgagat tttggcaaac ggtgaaatct cagaagagtg
    15661 tcgttctaat tacgccgtta attatttgta tttcctgaaa cagtgccaag aatacgccag
    15721 aaccaaccca atggattggt atcatctttg tttaactatt ttgaatgact gtattttact
    15781 tccgattgaa agtgacagtt taaatagtgc attaaccata ttttccacat taaacgatag
    15841 gggcttgcct ttgtcggatg cagatatttt taaagccagg ctttataaga tggcgcagca
    15901 gaaagataaa tttgttgaac aatggaagga acttgttgcg ttggcagagg gtgcatctat
    15961 ttccgtagat gatttgttcc gctattacac ccatgtatta agggcgaaag aaaagattac
    16021 ggataaagaa attgggcttc gccgttttta tactgacaac cgaaacaaac agttggaaaa
    16081 cgaaaatttg ttcaatcact taattgagct ggctggattt tggcaggata ttaacaacag
    16141 caaaactaac caagacgtgg aaacttcgaa ctttgtaaat gacgaggcgg ccaagtggct
    16201 gcaatgtctg aatgtctatc ctaatgagtt ttggaaatac gccgcgagtg tgttcttcat
    16261 tgcaaacaag ggtgcgcaat cctttaaaga tgattttgca gcattattaa aacagcttac
    16321 tgcatttttg tatgctaaat ttttagttgc gccaaccgta accgcaatta aagatgatat
    16381 ttataaatta tgtgttgcga tttatcacgg tgaaaaaaac gctttatcat atgttatgcc
    16441 tgcacccata cactatgaac aaaatacagg acgtataact aaagggttgt taatgattaa
    16501 tgcttatcta tttgatgaaa agcagaaatt attgccaact aaaactgaaa tcgaacatat
    16561 ctttccgaaa aaatggcagg aaactaacta taatggttgg tcaatcatag atgcggaaca
    16621 atatttagaa caactaggta ataaaattgt ttttgaaaaa cggttaaata ttcaggcagg
    16681 taacggctat tttggtaaga aaaaggagca atatcaaaaa tccgatattg ttgaagtgcg
    16741 tgaattagca ggtgaaaatg ccaatgattg gttaccgagt gatattcaga aacgccagca
    16801 gcagattgtg gatagattga aacagttttt cgataattgt tttgaagcag ccagcatccc
    16861 caaaaaaaat taacttttcc aataacctcc aaaccaaaca ggtaacgcaa tgttttctaa
    16921 ccatcttctc agcttggcaa tatggatacc catcgccgca ggtgtgctgg ttttggcaac
    16981 ggggtcggac agccgtgcgc tgcttgcccg cgtgctcgcc ttcatgggtg cgcttgccgg
    17041 tttcttggta acgctgcccc tgtttaccgg tttcgaccgt ttgagcggcg gctatcaatt
    17101 taccgagttc cacgagtgga ttccgctgct gaaaatcaac tatgcattgg gcgtggacgg
    17161 tatttcagtg ctctttatca tcttgaatgc gtttattacg ctgttggtgg tattggccgg
    17221 ttgggaagtt attcagaaac gtccggcgca gtatatggcg gcattcctga tgatgtcggg
    17281 tttgattaac ggcgcgtttg ccgcacagga tgcgattctg ttttatgtgt tcttcgaggg
    17341 tatgctgatt ccgctgtacc tgattatcgg tgtatggggt ggcccgcgcc gcgtttatgc
    17401 gtcggtcaag ctgttcctct atacgctgat gggttcgctt ttgatgctgg ttgccatggt
    17461 ttacctgtac tatcagacag gcagcttctc tattgtcgat ttccaaaaca tcaagcagat
    17521 tccattgggt gtacaacagc ttttgtttgt ggcattcttc ctgtcatttg ccgtaaaagt
    17581 gccgatgttc cccgtacaca cttggctgcc ggatgcccac gttgaagcac cgaccggcgg
    17641 ttcgatggtg ttggctgcga ttacgctgaa actgggtgcg tatggtttct tgcgctttat
    17701 cctgccgatt atgccggatg cggcacgcta ttttgccccc gtgatcatcg tattgagtct
    17761 gattgccgtg atttatatcg gtatggtggc tttggtgcaa accgatatga agaaactggt
    17821 ggcgtattcg tccatcagcc atatgggttt tgtaacgctt gggatgtttt tgtttgttga
    17881 cgggcagttg gacgactggg cgttgaaagg cgcaatcatt caaatgattt cccacggttt
    17941 tgtgtctgcc gcgatgttta tgtgcatcgg tgtgatgtac gaccgcctgc acacgcgcaa
    18001 tatcgccgat tacggcggcg tggtcaacgt gatgcccaag tttgcggcgt ttatgatgtt
    18061 gttcggtatg gcgaacgccg gtttgccggc gacttccggc ttcgtgggcg agtttatggt
    18121 gattatgggc gcggttaaag tgaatttctg ggtgggcgcg ttggcggcta tgacgctgat
    18181 ttacggcgcg tcttataccc tgtggatgta caagcgcgtg atttacggcg cgatccacaa
    18241 tccgcacgtt gccgaaatga aagacatcaa ctgccgcgaa tttgcgattc tggcgatttt
    18301 ggcggttgtg gtattgggta tgggtctgta tccgcaagcg tttatcgaag tggtgcatca
    18361 ggcggcaaac gatttgattg cccatgtggc acaaagcaag atttgaggtg tgtaaatgaa
    18421 ctggtctgat ttgaatttaa tgcccgccat gcccgaagtc gcgctgctgt cgctgctggt
    18481 gttattgttg ctggcagact tgtgggtcag tgatgacaaa cgccgttgga cgcattacgg
    18541 cgcgttggca acggtggcgg ttacggctgt ggtgcagctt gccgtttggg aacagggcag
    18601 cacgtcttcg ttcaacggca tgtatattgc agacggtatg tcgcgtttgg caaaaatggt
    18661 tttatatgcc ttgacgtttg ccctgtttgt ttatgccaag ccctacaacc aagtgcgcgg
    18721 tattttcaaa ggcgagtttt acaccctgtc gctgtttgcc ctcttgggca tgagcgtgat
    18781 ggtgagcgcg gggcattttt taactgccta tatcggtttg gaactcttgt cgcttgccct
    18841 ttacgccctg attgccctgc gccgcgattc cggctttgcc gccgaagccg ccttgaaata
    18901 ttttgttttg ggcgcgctgg catccggcct gctgctctac ggtatttcta tggtttacgg
    18961 cgcaaccggt tcgctggaat ttgccggcgt gctcgcctct tccttcaatg aagaagccaa
    19021 tgaatggctg ttgaaactgg gtctggtgtt tatcgtcgtc gccgttgcgt tcaaactcgg
    19081 cgcggtgccg ttccatatgt gggtgcccga cgtgtatcac ggcgcgccca cttctgttac
    19141 cgccttggtc ggcactgccc cgaaagtcgc cgccgtcgtt ttcgctttcc gcatcctcgt
    19201 taccgggctg ggaaccgtgc atcatgactg gtctctgatg tttgccctgc ttgccgccgc
    19261 ctcgctgctg gtcggcaacc ttgccgccat catgcagacc aatatcaaac gtatgctcgc
    19321 ctattccacc gtatcgcata tgggctttat cttgttggcg tttatggcag gcgcggtcgg
    19381 ctttgcggcg ggtctctatt acgccattac ctacgcgctg atggcggcgg cagggttcgg
    19441 cgtgttgatg gtgttgtcgg atggggacaa cgagtgcgaa aacatcagcg atttggcagg
    19501 gttgaaccaa caccgcgtat ggcttgcctt tttgatgctg ctggttatgt tctctatggc
    19561 gggcattccg ccgctgatgg gcttttacgc caaattcggc gtgattatgg cactcttaaa
    19621 acaaggctat gtttggttgt ccgtatttgc cgtcgtgatg tcgctgattg gcgcgttcta
    19681 ctacctgcgc gtggtcaaag tcatgtattt tgacgaatcc ggccgcgccc gtcccaccgt
    19741 cggcggcaac aatgccgcaa aattcctttt gagcgtcaac gcgctgctgt tggtgctgtg
    19801 gggcattatg ccgcaaaccg ttatcgactg gtgcgccaag gcgttggaaa acacgctgta
    19861 agccgccgca acggcagccg tgtcagaggc tgccgttttt gttaagatat gccgttccgc
    19921 aacgcagttc agacggcatc gccgccgaca atgcccaaac agaaagcccg ccatgaccgc
    19981 atccatgtac atccttttgc tgcttgcctt gatttttgcc aacgccccct tcctcacgac
    20041 caagctgttc ggcatcgtac cgctcaagcg caaacatttc ggacaccacc tgatcgagct
    20101 ggcggcaggt ttcgcgctga ccgccgttct tgcctacatc ctcgaatccc gtgcgggagc
    20161 ggtacacgat cagggttggg agttttacgc caccgtcgtc tgcctgtacc tgatttttgc
    20221 gtttccctgt ttcgtgtggc ggtatttttg gcacacgcgc aacagggaat aggcaggcat
    20281 aggaatgccg tctgaaaccc tttcagacgg catttgtttc attcaagtgt cggcacgttt
    20341 caatcggcga tatacgccgg tttcaatatt tgcgggcgac tgcaaattct gccaactgcc
    20401 gcaggcgcag ggctttgccg ccgaagggtt cgagcagcgc gaccgcttcg gcaaccagtt
    20461 tgtgtgcgta tgagcgcgcc gcttccaagc ccatcagttt cacataagtc ggcttgtcgt
    20521 tgtctgcgtc tttgcccgcc gttttgccca aagtcgccgt gtccgcttca caatccaaca
    20581 catcgtcaat gacttggaac gccagcccca gttttgccgc ataatcgtcc aataccgcca
    20641 gactgtcttc agacagattc gggcaggaca tcgcccccaa taaaaccgcc gcacggatta
    20701 gcgcacccgt tttcaggctg tgcatctgtt ccaaatcggc ttgaaccatt tgtttgccga
    20761 cattcgccaa atcgattgcc tgaccgcccg ccatgcccgc gctgccgccc gctttcgcca
    20821 acaccgacaa cattgccaac tggcgtgctg cgggcagttc tgtcggacgg cttaacacgt
    20881 cgaaggcttg ggtttgcaaa gcgtcgccga ccagtagcgc ggtggcttcg ccatatttga
    20941 tgtggcaggt cggtttgccc cgacgcaggc tgtcgttgtc cattgccggc atatcgtcgt
    21001 gaaccaacga ataggcatgg atcatttcga ttgccgccat tgcctgttct actgcttcat
    21061 gcacggcttc gcctaattcc gaagctgcca gaaccagcat cggccgcaga cgtttgcccc
    21121 catccaaaac cgcataacgc atggcttcgt gcagtgtgtg cggtatttcg ttttcagacg
    21181 gcaaaaagcg ttccagcagc agctctgttt gcgcctgcgc cctctgttgc cacgctttca
    21241 aatcattcgt cggattcaag gttcaactcc ttcagcccgt ccgcatccaa aacctgtaat
    21301 ttctgttcga cttgtgccag cttggtttgg cagtacctga ccagctcgtt gccttcctga
    21361 taggcggcaa gcgcgtcttc caagggcatt tcaccctgca tagactgcgt cagcgattca
    21421 aggcgcgaca gtgcctcttc aaacgatttc ggggcgtttt tcttcatagt ttcgggagtt
    21481 tctgtaaaaa cggaattgta gcatttctga aaaccggatg ccgtctgaaa cagcaggatt
    21541 tcagatggca tggtttttgc cctgcaaacg gcaagaggtt atttgagcag atcgtcggta
    21601 atgcgttgca ggaaggtcag ccgttcggtg cggattttac cggtttgcgc ggcggctttg
    21661 aaggcgcagc cgggttcggc gcggtgggtg cagttgtgga agcggcattg cccgacaagg
    21721 tggcggaaat cggggaaata gcgcggcaaa tcggcggctt ggaggtggtg taaaccaaat
    21781 tcttgcaaac ccggggagtc gatgagttgg gtttcgccgt tcaaatcata aagccgggcg
    21841 tgggtggtgg tgtgttttcc cgagtcgagt gcggcggaaa tgtcgccggt gcgggcggtt
    21901 tggctgccta aaagggcgtt ggtcagggtg gatttgccca taccgctctg cccgagcagg
    21961 atgttgctgt gcccttgcag gacggggcgc aggaggtcgg cgttttccag cgcacgggtt
    22021 tcgatgacgg gataacccag cgtttcgtag aatttgagtt tttcgcgcca aagggcggtt
    22081 tcgggcaggt cggctttgtt caggacgatg acggcttcaa taccggcggc ttcggcggca
    22141 agcagggcgc gttgcagcag ccgcacgctc ggactcggga cggcggcggt tacgatgagg
    22201 agttgggtaa cgttggcggc gatgagtttg gttttccacg cgtcttggcg gtagagcagg
    22261 ctttggcgcg gtaaaaaatc ttcaatcaca acttgttcgg cgttgacggg gctgatgcgg
    22321 acgcggtcgc cgcaggcgaa atcgacgcgt tttttgcggg tgctggcttc gtaggttgtg
    22381 ccgtcgggcg tgcggacaat gtagcggcgg ccgtagctgg cggtaatttg ggcggtgtcg
    22441 ttcatggttt ctttggggtt gggtgtggaa atgccgtctg aaaacgggtg ttcggacggc
    22501 atcggttcag tcgtgctgcc actcgacgtg ttcgttgagg aagccgccgc tctggtgcgc
    22561 ccagagtttg gcgtaaagcc cgcgtttttc gaggagttcg gcgtgtgtgc cttcttcgat
    22621 gatgcggcct ttgtcgagga cgacgagcct gtccattgcg gcgatggtgg agaggcggtg
    22681 ggcgatggcg atgacggttt tgccgtccat cattttgtcg aggctttctt ggatggcggc
    22741 ttcgacttcg gaatcgagcg cgctggtggc ttcgtccaaa agaagaatcg gtgcgtcttt
    22801 gagcatcacg cgggcgatgg cgatgcgctg gcgttgcccg ccggagagtt tcacgccgcg
    22861 ttcgccgacg tgtgcgtcgt agccgcgccg ccctttggca tcggaaaggt cggggatgaa
    22921 gccgacagct tcggcgcgtt cggcggcaga aaccatttcg gcatcggtcg cgtcggggcg
    22981 gccgtaaata atgttgtcgc gcacggaacg gtgcagcagc gaggtatctt gcgtgaccaa
    23041 accgatttgg gcgcgtaaag attcttgggt aacgccgctt atgtcctgcc cgtcgatcga
    23101 aaccgtgccg ctttgcggtt cgtagaagcg caaaagcagg ttgacgatgg tggatttgcc
    23161 cgcgccgctg cgtccgatca agccgacttt ttcgcccggg cggatggtga ggttgaagcc
    23221 gttgagcagc ggtttgcccg cttcgtagca gaaatcgatg tgttcaaatt tgattgcgcc
    23281 ttgcggcacg ttcagcggca gggcgttcgg cttgtcgagg atggtgtgcg gtttggacag
    23341 ggttgccatg ccgtcgccga cggtgccgat gttttcaaac agccgcgcgg attcccacat
    23401 aatgtattgc gacaaaccgt tgacgcgcaa cgccatggcg gtggctgtag caaccgcgcc
    23461 cacgccgacc tgcccgttgt gccagagcca gatgcccagt gcggcggtgg agagggtcag
    23521 ggaggtgttg acgatgaagc tgcacgaatg cagcagcgtc gccagccgca tttgggcgcg
    23581 caccgtaacc ataaattctt ccatcgactg cttggcatag gcggcttcac gcgcgccgtg
    23641 ggagaagagt ttgacggtgg cgatattgga ataggcatcg gtaatgcggc cggtcatcag
    23701 cgatcgggca tccgcctgcc atgcggcggt ttgccccaat ttgggaatca gcaggcgcat
    23761 caccgaagcg aaaccgacaa tccagccgat aaagggcagc agcagccatg agtcgagcga
    23821 ggcgagaatc acgccggagg taatgaaata caccgacaca taaacgacca tatcggcaac
    23881 cgtcatcacc gcgtcgcgca acgccagcgc ggtctgcatg actttggcgg acacgcgtcc
    23941 ggcaaattcg tcctgataaa aaccgaggct ttggttcagc atcaggcggt ggaagttcca
    24001 gcgcaggcgc atagggaaca cgccctgaag ggtttgcagg cgcacgttgg acgcggcata
    24061 cgcccacacg accgaaaaca ccatcatcgc cgccattgcc gccagttccc aacctttttc
    24121 ggcaaacagt tcggcgggcg cgtatttgcc gagccactcc acgattttgc ccataaattg
    24181 aaaaaccagg gcttccataa tgccgatgcc ggcggtcagc gcagccaggg cggctatcca
    24241 tttccgcacg ccggccatgc tgctccagac aaaccgccac aagccttttt ctggcgtttt
    24301 cggggcggct tcgggataag ggtcgattcg ggactcgaac caggaaaata ttttgttcaa
    24361 cattgttttc gatttcggta aaacagtttc agacggcatc aaacacaatg ccgtctgaaa
    24421 ggaaggacaa taacgccatt ttacgggaaa agccgtcggg aagacagcgc gaggcggaaa
    24481 cgcagggttt cgtcagggca aacgccgcgc cgccttcagg cggcattatt tcagcaggtt
    24541 tttcaaagca aggcgcacgc cttcgcccac gtccgtcccc tccggaacgc ctttgaccgc
    24601 cgcttttgct tcgcgttcgc tgtaacccag cgcaagcagc gtgctgacga tgtcttccgt
    24661 ttcgtcggcg gcgggtgcgg cggcaaacag cccgtccgtt accgtatgcg cgaccagctt
    24721 gccgcgcagt tccaagacca tacgttcggc ggtttttttg ccgattcccg gcgcggaaga
    24781 caggcgtttg acatcttctt cggcaaccgc ccgcgccagc tcgtctgccg tcatcgctga
    24841 caaaatgccc aaagccgttt tcgcgccgat gccgccgacc ttaatcagtt ggcggaaggt
    24901 cttgcgttct tccgcagtgg caaaaccaaa taaaagatgt gcgtcttccc gaatgataag
    24961 ctgggtaaac agttgtacgc tttcacccat gggcggcagg ttgtagaagg tctgcatcga
    25021 tacgtcggcc tcatagccga caccgttgac atcgatgacg atttgcggag ggtttttttc
    25081 aaccagtttg ccggtcagtc tgctgatcat gtgtgccgaa tcctgaagtg tcgggtgcaa
    25141 aatgccgtct gaaaccggtt cgggcttcag acggcacgga ttgtatcaaa ttcagtcgtc
    25201 gcggcgggag gaaatcacgc ggccggtacg ggcatcgaca acgactttgt attcctgtcc
    25261 gtttttgacg atttcgacat catagtgcgg acggccgttg tcgtgttcga gatcgatgtc
    25321 ggtgattttg ccgccgacac gcgccaacgc tgctttttcg gcttgggcgc ggctgatgat
    25381 tttgtcttgt ttgctgtatt ggtgtgcggc gtgtccgtgg tcgtcatcgc cgtgtccgtc
    25441 gtggtgggcg agtgcggggg cggaaatgcc catcagtgcg gttgcggcgg cggtcaagag
    25501 aaggcgtttg aagttcatgt tttgctcctg cgaagtatct ggtggtgttt gaaagacgta
    25561 aaggcgggac atcaaccggc agtcaatacc gcccgaacca taccgcgcgc ctgagcttcg
    25621 gcttcggcgg cacgttcctg cgaggcaaac gggcccattt tgacgacgta ttcgtaacgg
    25681 cgtttttcaa tggcgaggtt cgggcttgat gccgaagcgg cgaagttttg ggcggcttgg
    25741 ttcagatagg cttgtgcttc gtgttccgta ccgaaagatt tcaagtcgat gaagatgtct
    25801 ttgttttcgg caaccggtgc ggattggccc gggacgattt gttcgatttt gacgtgcgcc
    25861 gtcccttggt tgacaaagcc caatttttgc gcggcggctt tggatacgtc gatgatgcgg
    25921 ttgccgtgga aggggccgcg gtcgttgacg cggacgatga cgcttttgcc gtttttggta
    25981 ttggttacgc gcacatagct ggggatgggc agggttttgt gggcggcggt aaaggcgttc
    26041 atatcgtatc gttctccgcc ggaagttttg cgcccgtgaa acctgccgcc gtaccacgag
    26101 gcgttgccgg tttgcgtgaa ttcggcgact tggtttttcg gcgtgtagcg ttttccggcg
    26161 actttgtagc tgcggttggc ggaggcgtgc agtttttctg ccttgaccac tgcgtcggcg
    26221 gatgccgtct gaagggagtg tgtgccgaat gcggcggtga gaaggaaaag ggtttttcgg
    26281 gttaaagtca aaacgtgttc cgttcttgag ttgaagacga atgggcatca tgcccgccgg
    26341 atacgttccg aaccgccgta cagtgcggac ggcggttcgg aatgtgtccg gataggtttt
    26401 cagacggcat gaacttgcgt tcaaacgccg cctgcgtaac cgtgttgccg ccacgcttca
    26461 aagagaatca cggcaacggt attggaaagg ttcatactcc gactgccggg ctgcatgggc
    26521 aggcggattt tttgcgcggc gggcaggctg tcgaggatgt cggcaggcag tccgcgcgtt
    26581 tccggcccga acagtaaaac gtcgcctttt tgaaacgcgg tttcatcggg gcgcgccgtg
    26641 cctttggtgg tcagggcgaa aatgcgcctg cctgcgagtg ccttgaggca gtcgtcgaag
    26701 ttttcgtgca ccgtcaggct ggcgaactcg tggtagtcga gcccggcgcg tttcattttg
    26761 gcggaatcca atgggaagcc gagcggtttg acaaggtgca aatccgcgcc ggtattggcg
    26821 cacaggcgga tgatgttgcc cgtgttcggc gggatttccg gctggtataa aacgatggta
    26881 aacataaata tcaatcactt ataggcgcgt aaccttgcca caaggcggat ggggtgtcaa
    26941 aaaatttagt tattttttca ttggcgtgcg tgccagcgtc cagcagcaga ttcggtttgc
    27001 gcccgatttt ttcagcgtct ttgccaattc gtccagcgtc gcgccggtgg taaagacatc
    27061 gtcgattaac agaatattac agttttccgg tatcggtgtg cggatttcaa aggcgttttt
    27121 gatgtttcgc cgccgttcgc cgcctttgag cgtgctttgc ggcgggcggt ggtgtcggaa
    27181 aacggtgtgt cggggcagta tctgccagcc gtagcgttgt gccaacaacc cgacgatgct
    27241 ttcactttgg ttgaacccgc gttgcagcag ccgctccctg cttagcggta cgggcaggac
    27301 gaaatcgaaa cattcgtctg aaagccgatc aggcggattc tgcatcatca ggtttgccaa
    27361 cgggcgcgcc atactcaaat ctgccaagtg cttcagcgcg tgtatcatat tgctgacggg
    27421 cggttcgtaa tgcagcgaag cccacatccg gtcgaatgcg ggcggttttt tctgacagcc
    27481 gccgcacacc gatccgcctt gaacgtgcat gaaacacagg gggcagctgt ttgccgcgtc
    27541 ggtgcggtat gccgccaaat cgtcgcggca gccggcgcag atgccgtctg aaacgccgga
    27601 cgaaccgtgg cataatacgc aacgcctgat agtgggcgcg tctgcgatgc gccgccaacg
    27661 agagagaaaa tccatgcctg atgccgtcaa aaaagtttac ctgatacacg gttggggggc
    27721 gaaccgccac atgttcgacg atttgatgcc gcgcctgcct gcaacgtggc cggtgtccgc
    27781 cgtcgatttg cccggacacg gcgacgcgcc cttcgcgcaa cccttcgata ttgaggcagc
    27841 cgccgacgct gttgccgcac aaatcgatac gcccgccgac attctcggct ggtcgctcgg
    27901 cggattggtc gcgctgtatc tggcggcgcg ccatcccgac aaagtccgtt cgctctgcct
    27961 gacggcgagt ttcgcacggc tgacggctgc cgaagattat cccgaagggc ttgccgcgcc
    28021 tgcactgggc aaaatggtcg gtgcgttccg ttcggattat gccaaacata tcaaacagtt
    28081 tctacaatta cagcttctgc acacgcctga tgcggacgga atcataggca gaatcctgcc
    28141 cgatttggcg cgctgcggca cgcctcaagc cttgcaggag gcgttggacg cggcggaaag
    28201 ggcggatgcg cggcatttgt tggacaagat agatgttccg gtactgctgg tgttcggcgg
    28261 caaagacgcg attacgccgc cgcgtatggg tgaatatctg caccgccgtt tgaagggcag
    28321 caggttggtt gtgatggaaa aggcggcgca tgcgccgttt ttgagccatg cggaagcgtt
    28381 tgccgcgctg taccgcgact ttgtcgaagg ggtttgagat gaaccatcag gacgcacgct
    28441 ggcaggttca ccgccatctt gccgaacata ccgaccaacg cctgacactc gtccgcaacg
    28501 cgcccaagta tatcctgctt gcaggtgcgg atgcggacat cagccgcagc ctgctggcga
    28561 aacgctatcc gcaggcggta tttgaagaat acgattcccg tgcggatttt ttggcggcag
    28621 ccgccgcccg caaaggcggt ttttggcaaa ggtttgcggg taagggcgtg gtgcagcact
    28681 gccaatcccc gaccgcgccg ctgcccgaag cgtgtgccga tatgttgtgg tcgaatctcg
    28741 gactgttggc ggcggaacaa atccttcctg tgctgcacaa ctgggcgcgc gccttgaaga
    28801 cggacgggct gctgtttttt acctgcttcg ggcgcgacac ttttaccgaa ctgaaatgcc
    28861 gtctgaaaga aaacggcatt gaaagccgca gcgtgatgtt tcccgatatg cacgacttgg
    28921 gcgatatgct tgctgaaaac ggcttttacg accccgttac cgatacggcg aagctggtgt
    28981 tggattacaa aaaggcggaa acgttttggg cggatatgga cacgctgggc gtttggcggg
    29041 cgatggcgtg gaacgatgaa aacgccgcgc gttcgtgtgt cgggacaata tttgaacggg
    29101 aaggcggttt gagtattacg ctggaaacag tgtacggaca cgccgttaaa aaactgatgc
    29161 tgccgcaagg ggagaacgtg gtgcagtttt ttccgaagag atgatgtgca gatgccgtct
    29221 gaagccgttt ccaagtttca gacggcattt gtttgtgaaa accgacagaa ctaaaggaaa
    29281 tgccgatgta tagtgaatta aatttaaacc agtac
//
LOCUS       DAXHTY010000017        26827 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXHTY010000017 DAXHTY010000000
VERSION     DAXHTY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.017"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..215
                     /locus_tag="ACFNMK_000626"
     CDS             <1..215
                     /locus_tag="ACFNMK_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6363950.1"
                     /translation="MELETVVPPEEGEGEGEGADGEMPEYLELHGGREDDISAPSQPE
                     PPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            250..1761
                     /gene="ubiB"
                     /locus_tag="ACFNMK_000627"
     CDS             250..1761
                     /gene="ubiB"
                     /locus_tag="ACFNMK_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC6363951.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVCLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFADGRRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     DMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(2118..2936)
                     /gene="cysE"
                     /locus_tag="ACFNMK_000628"
     CDS             complement(2118..2936)
                     /gene="cysE"
                     /locus_tag="ACFNMK_000628"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC6363952.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
     gene            3120..3698
                     /gene="grpE"
                     /locus_tag="ACFNMK_000629"
     CDS             3120..3698
                     /gene="grpE"
                     /locus_tag="ACFNMK_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC6363953.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRTLANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(3822..4037)
                     /gene="nqrM"
                     /locus_tag="ACFNMK_000630"
     CDS             complement(3822..4037)
                     /gene="nqrM"
                     /locus_tag="ACFNMK_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC6363954.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(4063..5118)
                     /locus_tag="ACFNMK_000631"
     CDS             complement(4063..5118)
                     /locus_tag="ACFNMK_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6363955.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAYVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEQEKLSVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(5738..6955)
                     /gene="nqrF"
                     /locus_tag="ACFNMK_000632"
     CDS             complement(5738..6955)
                     /gene="nqrF"
                     /locus_tag="ACFNMK_000632"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC6363956.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVSVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(6969..7562)
                     /gene="nqrE"
                     /locus_tag="ACFNMK_000633"
     CDS             complement(6969..7562)
                     /gene="nqrE"
                     /locus_tag="ACFNMK_000633"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC6363957.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(7566..8192)
                     /locus_tag="ACFNMK_000634"
     CDS             complement(7566..8192)
                     /locus_tag="ACFNMK_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC6363958.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(8192..8968)
                     /locus_tag="ACFNMK_000635"
     CDS             complement(8192..8968)
                     /locus_tag="ACFNMK_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC6363959.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKNTDISKTFAERIEQRVVDLATGEYVKDAPKDFSARVAAKDPA
                     QSIQIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(8961..10193)
                     /locus_tag="ACFNMK_000636"
     CDS             complement(8961..10193)
                     /locus_tag="ACFNMK_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC6363960.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(10196..11539)
                     /locus_tag="ACFNMK_000637"
     CDS             complement(10196..11539)
                     /locus_tag="ACFNMK_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC6363961.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGCLFTTGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(11888..13309)
                     /locus_tag="ACFNMK_000638"
     CDS             complement(11888..13309)
                     /locus_tag="ACFNMK_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC6363962.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(13324..13671)
                     /locus_tag="ACFNMK_000639"
     CDS             complement(13324..13671)
                     /locus_tag="ACFNMK_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HFC6363963.1"
                     /translation="MNAVLKGIRANKRGGKPVSLQIYVKINSFFVFDRLFIKDIYLFV
                     LNKIIYQKEHIFPSTAIRLPAAFRFGKLVYPDYLLYNQSENFFQKQTGSTVRPCPGGT
                     VPVSLPRAAQGSA"
     gene            complement(13668..14027)
                     /locus_tag="ACFNMK_000640"
     CDS             complement(13668..14027)
                     /locus_tag="ACFNMK_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC6363964.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFYRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(14024..14629)
                     /locus_tag="ACFNMK_000641"
     CDS             complement(14024..14629)
                     /locus_tag="ACFNMK_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFC6363965.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(14676..15158)
                     /locus_tag="ACFNMK_000642"
     CDS             complement(14676..15158)
                     /locus_tag="ACFNMK_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC6363966.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      15362..15459
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            15670..16770
                     /gene="gcvT"
                     /locus_tag="ACFNMK_000643"
     CDS             15670..16770
                     /gene="gcvT"
                     /locus_tag="ACFNMK_000643"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC6363967.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAGVFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            16933..17319
                     /gene="gcvH"
                     /locus_tag="ACFNMK_000644"
     CDS             16933..17319
                     /gene="gcvH"
                     /locus_tag="ACFNMK_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC6363968.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNDDLPSAPETANSDPYGAGW
                     FFKIKPVNPADYDGLLTAEQYAGEVD"
     gene            17466..18713
                     /gene="hemA"
                     /locus_tag="ACFNMK_000645"
     CDS             17466..18713
                     /gene="hemA"
                     /locus_tag="ACFNMK_000645"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC6363969.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            19041..19562
                     /locus_tag="ACFNMK_000646"
     CDS             19041..19562
                     /locus_tag="ACFNMK_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
                     /protein_id="HFC6363970.1"
                     /translation="MSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAKI
                     QPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDVVNTRGYSSKPIDTLMALA
                     KDGTIAGAKLVDHHESIMLIGIPHLPAPGRAIRSNWLPAYIKPNFTSTNRLRLKGLPT
                     VLQPSKETGAGVP"
     gene            19451..20650
                     /locus_tag="ACFNMK_000647"
     CDS             19451..20650
                     /locus_tag="ACFNMK_000647"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HFC6363971.1"
                     /translation="MASGVYQTKLHIDKPITIEGPADRSATIEGDRSGRTIAVHAPDV
                     TLRNLTVTRSGMSLPAMDAGIYLEETAPRALIEHNNILDNSVGVYLHGSADAMVRENK
                     IVGDATLRVNERGNGVTVWNAPGAQVVGNDISKGRDGIFSNTSTHNTYKNNRFSDLRF
                     AVHYMYTNDSEISGNISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIH
                     DNIINKAGKCVFAYNANYDKLSANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKY
                     VSTRFLDWSEGGHGNYWSDNSAFDLNGDGFGDSAYRPNGIIDQIIWRAPVSRLLMNSP
                     AISIVKWAQAQFPAVLPGGVVDSKPLMKPYAPKIQTCYQAMKDELLKEAETRQSEWGR
                     AENGSLN"
     gene            20708..21322
                     /locus_tag="ACFNMK_000648"
                     /pseudo
     CDS             20708..21322
                     /locus_tag="ACFNMK_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(21360..21422)
                     /locus_tag="ACFNMK_000649"
                     /pseudo
     CDS             complement(21360..21422)
                     /locus_tag="ACFNMK_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            21418..21549
                     /locus_tag="ACFNMK_000650"
                     /pseudo
     CDS             21418..21549
                     /locus_tag="ACFNMK_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            21546..22040
                     /locus_tag="ACFNMK_000651"
     CDS             21546..22040
                     /locus_tag="ACFNMK_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC6363972.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTKWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVSFDDMPDT
                     YIFK"
     gene            complement(22174..23835)
                     /locus_tag="ACFNMK_000652"
     CDS             complement(22174..23835)
                     /locus_tag="ACFNMK_000652"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC6363973.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIAYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMINVNYKEYASPETRYCPAGVYEIIE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(23965..24543)
                     /locus_tag="ACFNMK_000653"
     CDS             complement(23965..24543)
                     /locus_tag="ACFNMK_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6363974.1"
                     /translation="MHYNPVAYGKIKVQSWKERRDFNIVKQDLDFSCGAASVATLLNN
                     FYGQKLTEEDVLKKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVY
                     LKYRKDDHFSVLRGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPK
                     GRDAGGDKAFFTRSPKRQTEFAVGQVKWWRAY"
     gene            complement(25580..26232)
                     /locus_tag="ACFNMK_000654"
                     /pseudo
     CDS             complement(25580..26232)
                     /locus_tag="ACFNMK_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         6524 a         6665 c         7163 g         6475 t
ORIGIN      
        1 atatggagtt agaaacggtc gtgccgccgg aggaaggtga gggcgaagga gagggcgcgg
       61 atggggaaat gcccgaatat cttgaacttc atggcgggcg ggaagatgat atttccgcac
      121 cttcgcaacc tgaaccgccg acccgcaata tcaaaccgct gcttttcggg ctgctgattg
      181 cctttttggg ctatctgctc ggtaagattt tttgattgtc cgataaatgc tgtattcggg
      241 attttatata tgaaatggtt gaaacgcctg acggtcattg tcgggacttt ttaccgctat
      301 cggctggcag gtctgtgtgc ttcgctgatg ggtagcggtt ggatatgcgc tctgctgaaa
      361 atgatgccgc agtcgtccaa attgaaaaac gaaccgcctg ccgtctgtct gcgccttgcc
      421 ttggaaagcc tggggccgat tttcatcaag ttcgggcagg ttttgtccac acgccccgat
      481 ttgattccgc acgattacgc cgtcgaactg gcaaagctgc aagacaaagt gccgcctttt
      541 gacgcgcggc tttcgcgtga acaaatcgaa aaatcgctgg gtcagcccat cgaaaagctg
      601 tatgcggaat ttgaaaccga gcccatcgcc agcgcgtcca tcgcccaagt acacaaagcc
      661 cgcctgcatt cgggcgaacg agtggctgtc aaagttttgc gccccaacct tttgcccctt
      721 atcgaacagg atttgtcgct gatgcgtttc ggcgcggctt gggtcgagcg tctgtttgcc
      781 gacggcaggc gtctgaagcc gcgcgaagtg gtggcggagt tcgacaaata tctgcacgac
      841 gagttggatt tgatgcgcga agccgccaat gccggacaac tcggacgcaa tttccaaaac
      901 agcgatatgc tgattgtgcc gaaggtgttt tacgactact gcaccagcga cgtactgacc
      961 atcgaatgga tggacggtac accggtatct gacatcgcta aactcaaagc agacggcatt
     1021 gatttgcaca aacttgccga ttacggcgtg gaaatctttt tcacgcaagt tttccgcgac
     1081 ggctttttcc acgcggatat gcaccccggc aatattttgg ttgccgccga caaccgctac
     1141 atcgccctcg atttcggcat cgtcggcacg ctgaccgatt acgacaaacg ttatctcgcc
     1201 atcaacttcc tcgccttctt caaccgcgat taccggcgcg tcgccaccgc ccacatcgaa
     1261 tcgggctggg tgcccgccga cacgcgcgcg gaagagttgg aagcggctgt ccgcgccgtg
     1321 tgcgaaccag tgttcaacaa accgatttcg cagatttcct tcggcttggt gctgatgcgc
     1381 ctgtttgaag tcagccgccg cttcaatgtc gaaatccagc cgcagctggt attgctgcaa
     1441 aaaacgctgc tcaacatcga aggcttggga cggcagcttg atcccgattt ggacttgtgg
     1501 aaaaccgcca aaccgttttt ggtgaaatgg atgaacgggc aggtcggccc taaagccctt
     1561 tggcgcaacc tcaaaaacga agcccccgac tgggcgcaaa tcattccttc attgccgcgc
     1621 aaaatcagtg cgttgattga tgaaaaccgc cagcaggaaa tgcgtgatgc ctatatccat
     1681 ttggtcaaag tgcagcagcg gcaaagcctg tggctggctg tgattgcggt tgttttgctg
     1741 ctgattttgc ttttgaaata ggctttgtcc gaatcatcgc ccgactccgc ccgtttataa
     1801 ggaaatcggt tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa
     1861 gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg attccgtact atccgtactg
     1921 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatatttcc ggttgcgtgg
     1981 gaatcgggtg tatcgaataa aaggcatttt gtccgactgg caagtgccga catcggcggc
     2041 atatcaaggc gcaggcttga agcgggcaat gtcgtctgaa gcccgtttgg cgtttcagac
     2101 ggcattggtg cggatattca aatcataaag tcgatttcgg caaactggat attttgatcc
     2161 atatccgccg acggtgtttt gagcgatcgc gccacgggtt tggcgggtac gccgacaacc
     2221 gtgatggacg gcggcacgtc tgaaaccacg acgctgcccg ccccgatttt ggcattgctg
     2281 ccgatgcgga tattgcccaa tatcgaggcg tttgcgccga tcatcacgcc gtcgccgatt
     2341 ttagggtggc ggtcgccgcc ttctttgccc gaaccgccga gcgttacgcc gtgcaaaatc
     2401 gaaatattgt tgcccaacac ggcggtttcg ccggcaacaa agccggtggc gtggtcgagc
     2461 atcagcccgt atccgaaacg ggcggcggga tggatgtcca cgccgaatac ttcggacata
     2521 cggttttgca ggaaatacgc cagcgttttg cgcccgtcga gatacagccg gtggttgatg
     2581 cggtgtgcct gaatcgcgtg gaagcctttg aaatataaaa gcggcagcga atattcgtcg
     2641 caggcgggat cgcgttcgta gatggctttt aagtctgctt cgacgcattt gccgatttgg
     2701 gtgtcgctgc ccaacgcctg ctggtagatt tcaaacagcg cgcgcacgtc cataatcggg
     2761 ctgccgagtt tgctggaaag gtggtaggca aggacggagc cgagggactc gtggcgcaac
     2821 acggtttggt gcaaaaaact tgccagcatc ggttcggcgg agaccgcggc cgcggtttct
     2881 tcgcggatgg tgtgccagag gtcgaaaccg gttgtgttta aatggtcttt tttcatgagt
     2941 gatgatgttt gaaaatcgat atggtcggca gtatcttacc gtctatatta ttttttcggt
     3001 aggggatttg aaaatgaatt tgaaattctc tgcttttgct tgaagtttct tgaaaatgtc
     3061 cttatcttgc gcgggtaata actggatttt gatttccaat ttgttttaag ggatacgata
     3121 tgagcgaaca gacacagcag caaaacagtg aagaagcggt tgaaaatgtg gaggcggtgg
     3181 aaaccgtcga gacagtagga aatgcggacg gtgtgcagga acaggctgcc gcagagccgg
     3241 cttatgagga tttgcaggcg cggattgccg agctggaagc gcagttgaaa gacgagcagc
     3301 tgcgcacttt ggcaaacgag caaaacctgc gccgccgcca ccagcaggaa attgcggata
     3361 cgcacaagtt cgccggacag aagtttgccg tggaaatgct gccggtcaag gattatctgg
     3421 aaatggcgct tttggatcag agcggcaatt tcgatgcgct gaaaatgggc gtgcagatga
     3481 ctttgaacga gttgcagaaa gcatttgatg ctacgcaaat caaggaaatc aatcctaaag
     3541 cgggcgataa gctcgatccg aatatccatc aggcgatgca ggcggtggca agcgaacagg
     3601 aacccaatac cgtggtgggc gtgatgaaga agggttatac gctgtccgac cgcgtgttgc
     3661 gcccggctat ggttacggtg gcacagaagg aagcctgaag gcgtctgggg aataatctga
     3721 tttatttcct gaagcgcgtt ttgcgtataa accgatcgaa gtaaagcggc aatgccgtct
     3781 gaacccgcct gtcgggcttc agacggcatt ttgtttcggt gttaacggtc aacccgtatg
     3841 ccgcctgcct gtttttcttc atccagtttc ttttgcaggg tgtcgcaagg tgtgtcgcag
     3901 tcgcacattt ttttcatacc caaggcagta atgccgccgc aactgccttt gatgctgcgt
     3961 ttggagaaaa tatagccgac cgccataccg atgatgacgg tcaggaagat gccgaaggta
     4021 aggagcaggg ttttcatggt gtttcctaat cggtttgtat gtttagcgga gcagtttttc
     4081 aaattcggaa gacatggcgg tgcggtagcc gcctttatcc ctgacaatca ggaaaacaga
     4141 gagtttttct tgttctgcca gctttaaggc ttcggtttcg cctaaaacaa ataatcctgt
     4201 ggacaagccg tccgccgtca tcgcgctatc tgcgaccacg ctgatggagg cgaggttgtg
     4261 gctgatgggt cgtttgttgt tcggattaat gatatgggag aggcgtttgc cgtttttatc
     4321 gacgtggaaa atacggtaat cgccggaagt ggcaagcgaa cggttgttca gcgggacgat
     4381 aatctgcgta ttgccgcctt ggacgatatt gggctgctcg ataccgatgc gccacggttc
     4441 gccgcgcgcg tttttgcctt tgccgtgcaa ctcgccgccg atttcgacca gataattttg
     4501 aatgccgtat ttttccagtt cgcccgcaac tttatcaacg ccgaagcctt tggcaatcga
     4561 agataaatcc aaataggctt tggggtgggt tttgctcaag gaggcgtaat ctttgccttg
     4621 tttcaaaatg attttgtcta tgcccgtata agatgctgct tgtttgattt gttccggcga
     4681 cggttcacgg gtaacggatt tgtcggggcc gaatccccaa aggttgacca aagggccgac
     4741 ggttacgtcc agcgcgccgt gtgtcaggcg gtttaggcgg acggcttcgg cagtaacgta
     4801 tgcgaagtcg cttgaaatgc ggaggggctt gccggctgtg tgttggttga accggctgat
     4861 ttcggagtcg ggctgatagg tggacatctg ccggttgact tctttaagcg cgtcatcgat
     4921 gcgcttttgt atttcggcag gggaggggag tttgtcccga ttgtttgaaa ggtatttgac
     4981 ggtataggtc gtgcccatcg tttcaccttg cagggtaacg gtttgcgcgg tttgttccga
     5041 acaggcgttc aggaagataa aactcagggc aaatatcaag gtgcggataa agttcggcag
     5101 gcgtgtttca gacggcatag tgtttgacgg ttttggcaaa tggtttgaat tatatcgcaa
     5161 aacggccggt atgtttctat gccgatgccg tctgaagggt gttcggacgg catcgcccgt
     5221 cgtttaggtg gcggtaatat agtggattaa atttaaatca ggacaaggcg acgaagccgc
     5281 agacagtaca aatagtacgg caaggcgagg caacgccgta ctggtttaaa tttaatccac
     5341 tataaaaaac aaaccggtgt tttaccaacc ggtttgtcaa ggtttaatgg tcagcgcgtc
     5401 ctgcgttgcc atttattgta gtagtattcg taatcttcat cacgaccgcc cgattcaatc
     5461 caatcatcgt attcggggct gttttcaaag ctgtcagaca ttatccgtac ctcgcaaaat
     5521 gggtttatcg atactttttt agttatggaa tcaaccataa ctaaaaagaa gtatacttct
     5581 ttgcttttcc gccagaatat ttaaccgcac atccgccgca gggtaaaaat ggaagttcaa
     5641 cgatattgat actggtatgg gcggatgccg tctgaagggt gttcggacgg tatcggataa
     5701 aaaaaggaag aaaccgaagt ttcttccttt ttgtctgtta gccgccgaaa tcgtccaaga
     5761 ggatgttttc gtcttccaca cccaagtctt tgagcatttt gatgacggac tggttcataa
     5821 tcggaggccc gcacatataa aattcgcagt cttccggcgc ttcgtgattt ttcaggtggt
     5881 tttcgtaaac cacgttgtga atgaagcctg tgtaaccgtc ccagttgtct tcaggcagcg
     5941 gatcggacag ggcgacgtgc cacgtgaagt tcgggaattc tgccgcgagt tggtcgaagt
     6001 cttcgacata gaacatctcg cgtttggaac gtgcaccata ccagaaggta attttgcgtt
     6061 tggagttcaa acgtttcagc tggtcgaaaa tgtgggaacg catcggagcc atacccgcgc
     6121 cgccgccgat aaataccatt tcggcatcgg tatctttggc aaagaattcg ccaaacggac
     6181 cggaaatcgt tactttatca ccgggtttga gcgaccagat gtaggacgac atttgtcccg
     6241 gaggcgcatc aggtacgcgt gggggcggcg tggcgatacg cacgttcagc ataatgatgc
     6301 ctttttcttc agggtatgaa gccatagagt aggcgcgcaa aatcggctcg tccactttgg
     6361 aaacgtattg ccacaaattg tatttgtccc agtcttcgtg atattcctta ggaatgtcga
     6421 agtctttgta ggcaacagtg tgaggaggag cttcaatttg aatgtagccg ccggcgcgga
     6481 aggggacttc ttcgccttcg ggaatggcaa gcttgagttc tttaatgaac gtggctttgt
     6541 tatcgttgga gatgacggtg cattcccatt ttttcacgcc gaacacttct tcgggcactt
     6601 cgatgtccat gtcggttttg acgctgactt ggcacgacag acggcagcct tcgcgtgctt
     6661 cgcgtttgct gatgtgggac agctcggtcg gcaggatgtc gccgccgccg ctttttacga
     6721 cgacgcggca ttgtccgcac gaaccgcccc cgccgcaggc ggaggggata aagatgcctt
     6781 cgttggcaag cgcgcccaag agtttgccgc cggcgggcat cgtcagctct ttttcgccgt
     6841 tgactttgat ggtgatgtcg ccttcgctga ccagtttgga tttggcaaat agaatcatca
     6901 gtgccaaaac caaaacgatg acggtaaaca tcacgatacc taaaataatc tccataccgg
     6961 tccctttctt ataactggat gccggagaac gacataaacg ccatcgccat caggccggcg
     7021 gcgataaagg taatgcccaa gcctttgagg cctttgggag cgtccgaata tttcattttt
     7081 tcggtaatgc ccgccaaagc gacaatcgcc aacatccagc ccaagcccgc gccgaagccg
     7141 tatacaacgg actcgccgaa gttgtattcg cgttgcgcca taaacgatac ggcgccgaaa
     7201 atcgcgcagt tcacggtaat cagcggcagg tagatgccca acgcgttata gagggcgggg
     7261 acgaatttat ccaagaacat ttccaaaatc tgcaccaaag cggcaatcac gccgatgaag
     7321 gtgatgaatt tcaaaaaggt caaatccacg ccttcgacaa tcgcgccgtc tttgagcagc
     7381 gagtaaacga gttggttgac agggacggac agcccgagta cgaaaattac cgccacaccc
     7441 aaaccgaatg cggtggatac ttttttggat accgccaaaa acgtgcacat acccaaaaag
     7501 aaggatagtg ccatattttc aatgaagacg gatttgatga agaggctcaa atagtgttcc
     7561 atagcttatt cctccgcctg ttcgggtttc caggtacgca gtccccaaat caaaaagccg
     7621 atgatgaaga acgcgctggg ggcgagcagg aacaagccgt tggtctgata ccagccgccg
     7681 tcctgcacgg tttggaaaac ggtgtagccc aagagtttgc ccgagccgat cagttcgcgg
     7741 acggtggcga cgacaagcag catcatcccg tagcccgcgc cgttgccgat gccgtcgatc
     7801 aggctttcca gcggcggctc tttcatcgca aatgcttcgg cgcggcccat cacgatacag
     7861 ttggtaataa tcagaccgac gaatacggaa agctgtttgg acaattcgta ggcaaatgcc
     7921 tgcaagagtt ggtcgaccag cgtaaccagc gacgcgataa tcgccatttg cacgataata
     7981 cggatgctgt tggggatgta gttgcgtacc agcgaaatga agaagctgga aaaaccggtt
     8041 accaaagcta cggaaatacc catcacgatg gccgtctgaa gtttggtggt aaccgccaaa
     8101 gccgaacaaa tccccaaaac ctgcaaggca atcgggttgt tgtcgataaa gggtgaaaac
     8161 attaagtgtt tcaagcgttt catatcagcc attattgcgc tcctgctgat ttcaatttgt
     8221 tcaggtaggg gatatagccg ttttcgccga accagtaggc gaacgaacct tgcacgcctt
     8281 tggatgtcag cgatgcgccg gagagggcat ctacgccgtg ttctttgtcc gaacccgcgc
     8341 ctttgccgac gtgcagggcg agtttgcctt gtccgtcaaa cagttttttg ccgacgaatt
     8401 tttgctgcca caaaggattg ccgatttcgc cgcccaagcc cggggtttcg ccttgttcgt
     8461 agtaggtaat gccattgatg gtgttgccgt caggttggat ggcgacgaag ccgtacatga
     8521 ccgaccacaa accgttgccg tgcataggca ggatgatttg cccgattttg ccgtcttcac
     8581 cttttaccaa ataaacctca gtgtatttgg cacggctttt gatgcctgcc aaatcgtctt
     8641 cgggtttgat ttggatgctt tgcgcgggat ctttggcagc aacacgcgcg ctgaagtctt
     8701 tcggcgcgtc tttcacatat tcgccggtcg ccaaatcgac aacacgttgc tcgatacgct
     8761 cggcaaaggt tttgctaatg tcggtgtttt tatccatcaa accggctacg ctcaagatat
     8821 agccttgttt gtcttggagt ttttgtttct cttggatggg tttcaagccg acgaccgcac
     8881 cggcaacgat gaccgagcaa atcaggctga ccgccaacac gacaatcagc gtgccgctga
     8941 agctgtcttt atcgaatttc ttagccattg ctgcgcgcct ttctgcgttt gatgttcgct
     9001 tgtgcgacga aatagtcgaa aatcggggca aacaggttgg caaacagaat cgccaacatc
     9061 atgccttcgg ggtaagccgg attgaccacg cggattaaca cgcacatcac accgatcagc
     9121 gcaccgtacc accatttgcc gacattggta aaggaagcgg aaacagggtc ggtcgccata
     9181 aacagcatac cgatggcgaa gccgccgacg accaaatgcc agtaccaagg catagcaaac
     9241 atggcgttgg tgtccgaacc gatgaagttg aacagcgaag acatcgcaat cataccgatc
     9301 atcacgccgg caataatgcg ccaagaagcg atgcgggcaa acacgataaa cgcgccgccg
     9361 attaagagtg ccaaagtgga gacttcgccg atggagccgg gcagtttgcc gataaacgcg
     9421 tccatccaag tgatggtttg accggttacg gcgtttttca ggccgtctgc accgtgtgcc
     9481 gcccattgcg ccagcgcggt tgcgccggaa tagccgtcaa ccgccgtcca aaccgcatcg
     9541 ccgctcaagt tggcggggta ggcgaagaac aggaaggcgc ggcctgccag cgcagggttc
     9601 ataaagtttt tacccgtacc gccgaatact tctttcgcaa ccacaacgcc gaaagaaata
     9661 cccaaagccg cctgccacag cggcagcgtg ggcggaacga ttaaggcaaa cagaatcgag
     9721 gtaacaaaga aaccttcgtt gatttcgtgt ttgcgcacgg tggcgaacaa gacttcccaa
     9781 aaaccgccca cgacaaatac ggtcgcgtaa atcggcagaa agtaaatcgc gccgaacagc
     9841 attttgccca acacgcccgc ttcagacgac atattgatgc ccaaagcgtc ggcaagggtg
     9901 taatgccagt cgttggcgat gctttgttgc agcaaatcgg gcgttaaggc accgaatgcc
     9961 tgtgcgccga cgttgtacat gccgtaaaac atggcgggga acaaagccag ccacaccaaa
    10021 atcatcatgc gcttggagtc gagcgcgtcg cggacgtgcg ccgctttgcg cgttaccgca
    10081 ccggatgtgt agaaaatcgt cgccgccgct tcgtagaggg cataccattt ttcatgtttg
    10141 ccgcccggca ggaagtgcgg ttcgattttt tccagaaaat gtttcaagcc cataatcagc
    10201 cttccttctc aatggtttcc agcactttgc gcaacagcgg gccgtattcg tatttgcccg
    10261 ggcagacgaa gctgcacaaa gcgaggtctt cttcgtccaa ttccaagcaa cccaatgcct
    10321 gcgcgctgtc ggtatcgccg acgattaaat cgcgcaaaag cagggtgggc aggatatcca
    10381 agggcatcac gcgctcgtaa gtgccgatgg gaaccatagc gcggtcgccg ccgttgacgg
    10441 ctgtcgtgaa cttgaagagt ttgtttttca ggaaatggcc gagggtcgtc cgcgtgatgg
    10501 agtatttgtc cggctgcggc gcaacccagc cgaacagctc tttgctgcgg ccttcttcga
    10561 taacggaaat ctgattgtgg tagcgtccca aataatcatg cgcgccttgt gcaatcgcgc
    10621 cgttcaatac tgagccggaa atcacgcggt tgtccgcgtc aaccaattcg cccgcagtaa
    10681 tttgcgatac tttcgcaccc aaaacggtac gcaagaggcg tggtttgttg acttgagaac
    10741 cacccaaggc aatcacgcgt tcggtgttca gacggcctgt tgtaaataaa cagccaatgg
    10801 caatcacatc ttgataattg atggtccaaa cggttttgtt tgcaccgacc ggctcgatga
    10861 aatgaatgtg cgtgccactc aaaccggccg gatgcgggcc gccgaattca tgtgtttcga
    10921 tgttggcagc attttcagac ggcacgtctg cgccagctgc cttacacaca tggattttac
    10981 gctcggtcaa acggctcaat accagcaaac ctcgtctgaa atcctcggcg gcttctttga
    11041 tcacaaccac agggtctgcc gccagcggat tggtgtccat cgcattgacg aagatggcga
    11101 acggctcggc atcgacggca gggattttgc tgaacggacg ggtacgcagc gcagtccaca
    11161 aaccggattg gatcagattg cggcgcactt cttcgccgct taagtttgcc aacgcttcgg
    11221 gcgcgtagcg ttcgaactcg atttcgtcgt tgccttcaac ggcaatcacg accgactgaa
    11281 gtacgcgctt ttcgccgcga tggatggcgg cgattttgcc tgaaaccggc gcggtaaaca
    11341 ccacgcccgg attctttttg tcttcaaaca gcacttggcc ttttttgacg gcatcgcctt
    11401 ccttgacttt catcgagggg cgcataccgg catattcttc gccaagcaac gcgacttcgg
    11461 taatggccgg gccgtcataa atgacttgct ccggtctgcc cgcgatgggc aggtttagac
    11521 cttttttgat tttaatcata tatttgcatt acttgtgatg gttaaggtaa aaacggcgtg
    11581 ttttgatacc gtgtcgcgtg gcatcaaaag cattgaataa attaatgtag caaagtgtta
    11641 gattctatca ggaattgtac ctgtttgtca gatttgctgc ttttttcctt gcggaagccg
    11701 tttttatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgcc gtactatttg
    11761 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata attgtcggaa
    11821 aggaaaaata ttgatttgat tatgccggaa tttaaaatgc cgtctgaatg ttcagacggc
    11881 atagcgttta cagcagtttg aaaacgaaaa agataagggt atgtacgatg aagacgggtg
    11941 tcaggaaggc gaccgaccac atcatatagc cgaagaaagt cggcatcggt acgccgcgct
    12001 gttcggcaat ggccttgacc atgaagttcg gtgcgttgcc gatgtaggtc agtgcgccca
    12061 tgaataccga acccatagaa accgccagca gcgaatgaaa cagggtaccc gtcatcaagg
    12121 cttgggcatc gccgcccgcc atattgaaaa aaacgagata agtgggcgcg ttatccaaga
    12181 atgccgacaa tatgccgctc atccaaaaat acatcacatt aatcggatga cctgccgtat
    12241 cgtgaaccag cgataccacc ccgcccagcg cgcctgcctc gcctgctttc agaatgctca
    12301 ggacgggaaa gatggtgatg aagatgccga ggaagagttt gcccacttcg gcgatgggtt
    12361 caaagttgaa ttcgttgcct gcgcggactt gtttgggcgt gattgccata gatacggcgg
    12421 tcaatgcaat caggatgaca tcgcggacga ggttttgcag ggcgtaacgg ctgccgagga
    12481 tttcaaatcc cgggtgttcg ggtttccaaa ggccggacat tagaaccgcg ccgaccacgc
    12541 ccgaaagcag gaggaagttc catttgccga agatggcgat tttttcgggt ttttcctgtt
    12601 gtgccggcgt atcttgtgca atgctttcct gtttgaagaa acggttgtcg atgaaataga
    12661 aggcggtcaa caggacagcg gtgctgatca ggacgggggc gaacatatgt ttgaccgtcc
    12721 acatgaaatc tacgcctttg aggaagccga ggaagagtgg ggggtcgccc aaaggggtca
    12781 gaccgccgcc gatgtttgca accaggaaaa tgaagaagat gacgatgtgc acgcggcggg
    12841 tacggttttg gttggctttc agcagcggac gaatcatcag cattgctgcg ccggtcgttc
    12901 ccatgataga ggcaagtgcc gtaccgacgg caagcagggc ggtgttgagc ttgggtgtgc
    12961 cgttcaagtc gccccaaacc aaaatgccgc ctgaaatggt gtacagggca agcagcagca
    13021 ggatgaaagg gatgtattct tcaacgagtg cgtgtgcgac ggtatggata ccggcggacg
    13081 cgccaaaaac caaactgaac gggatgagga agagcaatgt ccaaaaggcg gtaattttgc
    13141 cgtaatggtg atgccaggta tgcgaaaaaa acaagggacc caatgcgata gacagcaaaa
    13201 tcagggcaaa gggcaggccc cacagcaggt ttaggtttgc gccgtccaaa tctgcggcgt
    13261 aaaccgatgc tgggaaaagc attagtgaaa acaggggtag gtggcgcatc gtgtttcctc
    13321 gattcaagca ctgccttgcg cggcgcgtgg gagtgataca ggcaccgtgc cgcccggaca
    13381 tgggcggact gtgctgcctg tttgtttttg aaaaaagttt tccgattgat tgtaaagtaa
    13441 ataatcagga taaaccagtt tcccaaaccg gaaggcggcg ggaaggcgga ttgctgtgct
    13501 tgggaatata tgttcttttt gataaataat tttatttaaa acaaataaat atatgtcttt
    13561 aataaataat ctatcgaaaa cgaaaaatga atttatttta acatatattt gcaatgaaac
    13621 aggtttgccc ccccgtttgt ttgcccttat ccctttcagt acggcattca agattcgggc
    13681 ctgcgccaca tccatatggc gacaagggaa caaaaaaccg atgaaaccgc cccgacccac
    13741 cagcgttggg gaaactgcca aaacattatc aggcaggatg cggtcatcat actgatggcg
    13801 aatattttgg ctttgcgcgg cactgcgccg ttttgttccc agttatgaac catcgggccg
    13861 aaatagcggt gccggtgcag ccagcggtaa aagcgcgggg atgcctttgc ccagcaggcg
    13921 gcggagagca gtacgaacgg cgtggtcggc aacagcggca aaaaaatgcc gatgataccc
    13981 aacagcaggg agatgcagcc gcaggcaatt aaaagataac gtatcatttt gaaatatttt
    14041 tcttattgtg cggataaggg caggatgtga taccgagttt tgcccagcct tcatgtccca
    14101 ttttttccag cagggcgata ttgcgttcga atattgccga agcgtcggga aaggcttgtg
    14161 cggctttggc aatgctgtct tcgcggatga ggtgcagcgt cggataggga gaacggttgg
    14221 tgtagttgcc gatgtcgtcg ggttcggtat tctgaaagcg gaattgggga tggaacggtg
    14281 cgatttggac gatgccttcc aagccgtttt cgataacggc ggcatcggca atatcgagca
    14341 tatcgttgaa tacgtcgaaa tcggggaata gggtcgggtg aaccagcagg gtggtttcca
    14401 gttcggtggc gggtgtattg cccagtcgct gcagttcttc gtccaagtct tccaaaaaac
    14461 tgtcaaggtg tttggcttcg ctgattgcga tgcggacaag gtttttaacg tggggggctt
    14521 tggcaaaggg acacaggttc agaccgatga cggctttttc caaccattgt ccggtgtgtt
    14581 cggcaacagc atctttattt tcggaagtat tgatattcat tattgtcatg taaatgtgtt
    14641 tgcagattgc acgtgcggga aaatcgggaa gggcactatt ccttcagcag gtggttgagc
    14701 ggcagggagg tggtgtgttt gatttctttt aaaacaaagc tcgattgcgc atcttgtacg
    14761 ccgtggtggg acaggagcgt atccaaaaca aaatgggaaa acgcgttcat atcggtaaaa
    14821 aacgcctgaa gcaggtagtc ggtttcccct gtcagggcga agcagctcaa gacttcaggc
    14881 cattttcgaa ccgatgcggc aaagtcttcc cgcgcgtctt ttgctttgcg gatggaaacg
    14941 cggataaatg cctgaagccc caggttgacg gattccggag acagcagcgc ggcatattgg
    15001 cggacgatgc cggcatcttc caactgcttc agacggcgca ggcacggaga aggcgaaagt
    15061 gcgacacgtt cggacagttc gacattggtc agcctgccgt tttcctggag aacctgtaag
    15121 attttaatat cggttttgtc taaagtgagt tggggcatat ttgcgttccg ttttaaggaa
    15181 ttcggattgt ctgtccgtat gtttgcggca atccgcacag atggagacca tattaacata
    15241 taaaaagtta taccgtcatc cgggacaaat tttgttttcg gaaaatcatg tgaaaacaga
    15301 ggcggtcggt ttgcatctct tttaagacgg cttgcccaaa ccgccgattc aagacataat
    15361 cgggaaatgt gcaggagagt gttacaccca actacaatgt aaccaccgaa ggcgcagaca
    15421 cccttaaatc gctcaggtat cagggactgc acattgaaac aaacaatctg gagagcggcg
    15481 ttggaataac gtccaccgaa ggggagaagg ccgtctgaac caccattcag acaaccgcgc
    15541 aaagcagtga gcagactggt ttgccatcat gcggatacgg ccgaaaatct caggttcaag
    15601 gacagatagg gtcatccgcg cacaggtgcg cgggtggcat ctgaacaaaa aatccggaga
    15661 aacttgagaa tgattactct gaaaaccacc ccatttcatc aagcccatca agatgcaggc
    15721 gcgaagccgg tagattttgc cggctgggag ctgcccatcc attatggttc gcaaatcgcc
    15781 gaacacgaag ccgtgcgcac cgacgccggt atgtttgacg tatcccatat gcttgttacc
    15841 gacgtagcag gcgcaaatgc caaagccttt ttccgcaaat tgattgccaa cgatgtcgcc
    15901 aagctcgctt ttgtcggcaa agccctttat tccgctttgc tcaacgacaa cggcggcgtg
    15961 attgacgact taatcgttta ccgcaccaac gaagccgaaa cccaataccg catcgtgtcc
    16021 aacggagcga ctcgcgaaaa agacacggcg caattccaca aagtcggaca agagttcggc
    16081 gttgccttta atccgcgcta cgacctcggc atgctcgccg tacaaggccc taaagccatc
    16141 gaaaaactcc tgaccgtcaa acctgaatgg gcggatgtga ttcacggcct caaaccgttc
    16201 caaggtgcag acttgggcaa cgactggttt gtcgcccgca ccggctatac cggcgaagac
    16261 ggcgtcgaag tgattctgcc gggtactgaa gcaggcgtat tcttcaaagc cctgcaagca
    16321 gcaggcgtac agccttgcgg cctcggcgca cgcgacaccc tgcgcatgga agccggtatg
    16381 aacctctacg gcaacgatat ggacgacgac accagcccgc tcgaagcagg catgggttgg
    16441 acggttgatt tgaaagacga aagccgcgat tttgtcggta aagccgcctt gctggcattg
    16501 aaagaaaaag gcgttgccgt caaacaggtc ggtctgttgc tcgacaaagg cggcatcctg
    16561 cgcgcgcata tggaagtgtt gaccgacaaa ggcaaaggcg aaaccaccag cggcgtattc
    16621 tctccaagtc tgaaacaatc catcgccatc gcccgcgtgc cgaaagactt tgacggcgat
    16681 accgccaaag tgctgatgcg tggcaaggaa gtggacgtgc gcgtattgaa gttgccgttc
    16741 gtgcgtaacg gtcaaaaaca gtttgattaa tatcggttca gacggcattt tcatttcata
    16801 tgccgtctga aagcaggttt taattgtcgt ccgatacgga tatttgtaga aagcattgaa
    16861 caaggcatct gtggatattg attcatgcag atgccgtctg aaaataaccc cctaccaatg
    16921 gagtatcaaa ccatgagcaa caacatcccc gccgaactga aatacgttgc cagccatgaa
    16981 tggctgcgcc ttgaagaaga cggtacgatt accgtcggca ttacccacca cgcgcaagag
    17041 ctgttgggcg acatcgtgtt cgtcgagctg cccgaagtcg gcgcgaacct tgccgctgaa
    17101 gagcaagccg gtgtggttga gtctgtaaaa gccgcgtccg acgtgtacgc accgattgca
    17161 ggtgaagtcg ttgccgtcaa cgatgactta cccagcgcac ctgaaactgc caacagcgac
    17221 ccttacggcg caggctggtt cttcaaaatc aaacctgtca atcctgccga ttacgacggt
    17281 ctgctgactg ccgaacaata cgcgggcgaa gtggactaaa tctctatcct gataatggga
    17341 tgactgattt gagaaactgc actttcagac ggcaaggatg cgtttggaag tgcagttttt
    17401 gtctgtgtct tcaaagttca gttaggctat aataacggtc tatattcatc tttaccgatt
    17461 ttttcatgca acttaccgct gtcggactca atcatcaaac cgcaccttta agcatacggg
    17521 aaaagctggc gtttgccgcc gccgccctgc ctaaagccgt ccgcaatctt gcccgaagca
    17581 atgcggcaac ggaggcggta atcctttcta cctgcaaccg caccgagctt tactgcgtcg
    17641 gtgattcgga agaaatcatc cgatggcttg ccgattacca cagtttgccg attgaagaaa
    17701 tccgtccgta tctgtacgcg ctggatatgc aggaaaccgt gcgccatgcc ttccgcgttg
    17761 cctgcggctt ggattcgatg gtgttgggcg agccgcagat tttaggacag attaaggatg
    17821 cggtcagggt tgctcaagag caggaaagta tgggtaagaa actcaatgcc ctgttccaaa
    17881 aaaccttttc tgttgctaaa gaggtccgta ccgataccgc tgtcggcgag aactcggttt
    17941 ccatggcttc cgcttccgtc aagttggcag agcagatttt ccccgacatc ggcgatttga
    18001 atgtcttgtt tatcggtgcg ggtgagatga ttgagctggt tgccacttat tttgccgcca
    18061 aaagtccccg gctgatgacg gttgccaacc ggacgctggc gcgtgcacag gagttgtgcg
    18121 acaagctcgg tgtcaacgcc gaaccgtgcc tgctgtccga tctgcctgcc attctgcacg
    18181 attacgatgt ggtggtttct tcaacggcga gccagcttcc gatagtcggc aaaggcatgg
    18241 tggagcgtgc attgaaacaa aggcagagta tgccgttgtt tatgcttgat ttggccgtgc
    18301 cgcgtgacat tgaggcggaa gtcggcgatt tgaacgatgc ctatctttat acggtggacg
    18361 atatggtcaa catcgtccaa agcggcaagg aggcaaggca gaaggccgcc gccgccgccg
    18421 aaacgctggt atccgagaag gttgccgaat ttgtcaggca gcagcagggc aggcagagcg
    18481 tccccttgat taaagccttg cgggacgagg gcgagaaagc gcgcaagcag gtgttggaaa
    18541 atgcgatgaa acagcttgcc aaaggcgcaa cggcagaaga ggttttggaa cggctgtccg
    18601 tccaactgac caacaagctg ctgcattcgc cgacccaaac tttgaataag gcgggggaag
    18661 aagataaaga tttggttcat gccgtcgcgc agatttatca tttggacaaa taacggtgcg
    18721 ccggaaaagg atgccgtctg aaggggtttc agacggcata tttttgtgct gtctgacaac
    18781 atcgtgaaat cccacattat atcgatgtaa tcacaaagta tagtggatta acaaaaatca
    18841 ggacaaggcg acgaagccgc agacagtaca gatagtacgg caaggcgagg caacgctgta
    18901 ctggtttaaa tttaatccac tatattatcc cgtatgcgga ttggttttaa gatttgtaaa
    18961 tttgatttgc atcaaaaaat cgccgataga tgattcatat aatatcaata ttaaagagta
    19021 tcggtatatc ggggatagtc atgtcctgtt tttcaatcaa acgtatgtcc gcgtttcggg
    19081 cgcggataac ggcgtttttt gccgcctttg tctttttgac ggcggcactg cccgcttatg
    19141 cggagcgtct gcctgatttt ctggcgaaaa tacagccttc ggaaattttt ccgggtgcgg
    19201 accgttacgg caagccggaa ggtaagccta tggttgcccg cgtttacaaa ggcgatgagc
    19261 agctcggttt ggtctatatc acaaccgatg tggtcaatac gcgcggttat tcgagcaaac
    19321 cgattgatac gctgatggcg ttggctaaag acggtacgat agccggagcg aaattggttg
    19381 atcaccatga gtcgattatg ctgatcggta tcccgcattt gcccgcgccc gggcgggcga
    19441 tacgctcaaa ctggcttccg gcgtatatca aaccaaactt cacatcgaca aaccgattac
    19501 gattgaaggg cctgccgacc gttctgcaac catcgaagga gacaggagcg ggcgtaccat
    19561 agccgtacac gcgccggacg taacgctccg caacctgacc gttacccgtt ccggtatgag
    19621 cctgcctgca atggatgccg gtatttatct cgaagaaacc gccccgcgcg ccctgatcga
    19681 acacaacaat attttggata attcggtcgg cgtatatctg catggttctg ccgatgcgat
    19741 ggtgcgcgag aataaaatcg tcggcgatgc gactttgcgc gtgaacgagc gcggcaatgg
    19801 cgttaccgtt tggaacgcgc ccggcgcgca ggtcgtcggc aacgatattt ccaaagggcg
    19861 ggacggcatt ttttccaata ccagcacgca caacacctat aaaaacaacc gcttcagcga
    19921 tttgcgtttc gccgtccact atatgtacac caacgacagc gaaatcagcg gcaatatttc
    19981 cgtgggcaac aatatgggct atgtgctgat gttttccgag cggctcaaag tgtttgacaa
    20041 tatcgccgtc ggcagccgcg accaaggcat catgctcaac tatgtcaact attccgatat
    20101 tcacgacaac attatcaaca aagcgggcaa gtgcgttttt gcctacaatg ccaactacga
    20161 taaactgtcc gccaatcatt ttgaaaactg ccaaatcggc atacacttta ccgccgccat
    20221 cgaaggcacg tccctgcacg acaattcctt tatcaacaac gaaagccagg tcaaatacgt
    20281 cagcacgcgc tttctcgact ggagcgaggg cggacacggc aactattgga gcgacaacag
    20341 tgcgttcgat ttgaacggcg acggcttcgg cgacagcgcg taccggccca acggcatcat
    20401 cgaccaaatc atctggcgcg cgcccgtatc gcgcctcttg atgaacagtc ccgccatcag
    20461 catcgtcaaa tgggcgcagg cgcagtttcc cgccgttttg cccggcggcg tggtggacag
    20521 caaaccgctg atgaagcctt atgcccccaa aattcaaacc tgttatcagg cgatgaagga
    20581 cgagctgctc aaagaagccg aaacgcggca gtcggaatgg ggcagggcgg aaaacggttc
    20641 tttgaactag tctgcttcag acggcatccg gattcaaatg ccgtctgaaa acacaaaagg
    20701 aacaaccatg accacacatc atgtcgaatt gaggaaggta accaaacgct tcggggcgca
    20761 aaaagccgtt aaccaagtcg atttggtttt gaaggcagga gaaagcgtcg ggcttgccgg
    20821 acacaacggc gcgggcaagt ccaccattat gaagctgata ctcgggctga ttaccccgac
    20881 cgaaggcgaa gtgatgcttt tgggcgaacg taccggcagc aaagcggggg cgcggcttcg
    20941 cagccaaatc ggctacctgc ccgaaactgt tgcgttgcac ccttcgctga ccggcatcga
    21001 aacgctggat ttttatgcca aacttaaaaa acagccgctc acgcagaatc gggggctgct
    21061 cgagcgcgtc ggcatttcac aggcggcaca ccgccgcgtc ggcacttatt ctaaagggat
    21121 gcgccaacgc cttgccttgg cacaagccct gctgggcgag cccaaagtcc tgctgtttga
    21181 cgaaccgaca accggcctcg accctgcatc aaggcaaatg ttttacgaag tcgtgcgcga
    21241 actcaacggg cgcggcgcga ccgtattgct cagtacccac gcccttgccg agttggacgg
    21301 acacgccgac cgcattatcg tggattaaat ttaaaccagt acggcgttac ctcgccttac
    21361 cgtactattt gtactgtctg cggcttcgtt gccttgtcct gatttaaatt taatccacta
    21421 tatgcgggta tggcgggttt gagcggacaa atcagcctga ccgtccccgt tttgctgacc
    21481 gctcaggttt tatgggttat cattccgctt gttttggcag ccggaatttt tagaaagcga
    21541 caaatatgaa aaaaaccctg ttggcaattg ttgccgtttc cgccttaagt gcctgccggc
    21601 aggcggaaga ggggccgccg cctttacccc ggcagattag cgaccgttcg gtcggacact
    21661 attgcagtat gaacctgacc gaacacaacg gccccaaagc ccagattttc ttgaacggca
    21721 aacctgacca gccagtttgg ttctccacca tcaagcagat gttcggctat accaagctgc
    21781 ccgaagaacc caaaggtata cgcgtgattt acgttaccga tatgggtaat gttaccgatt
    21841 ggacaaatcc caatgccgac acgaagtgga tagatgcgaa aaaagccttt tatgtcattg
    21901 acagcggttt tatcggcggt atgggtgcgg aagacgcact gccgttcggc aacaaagagc
    21961 aggctgagaa atttgcaaag gataaaggcg gtaaggttgt cagtttcgac gatatgcctg
    22021 atacctatat tttcaaataa atccaaacaa aaatgccgtc tgaacgttga agccaaggtt
    22081 cagacggcat tttttgtcat aggttcaaac ggggcaggtt tgaacaactg ccccattcct
    22141 tattcctgac cgggcgtaac ggcggaataa ggtttacatc ccgccgtaat tcggcccgct
    22201 cgcgccttcg gggcaaatcc aagtgatgtt ttgcgtcggg tctttgatgt cgcaggtttt
    22261 gcagtgcacg cagtttgccg cgttgatttg caggcgcgga ttgccgtttt cttcaataat
    22321 ttcgtacaca cccgccggac aatagcgcgt ttcgggcgag gcgtattctt tgtagttcac
    22381 gtttatcatc gtttgcggat tgttcagcac caaatggtcg ggctggtttt cttcgtgcgc
    22441 gagattggca aggaagacgc tgctcaagcg gtcgaaggtc aacacgccat cgggtttcgg
    22501 ataagcaatc ggcttgcacg cggcggcttt tttgagctgc tcgttgtctt taccgtgatg
    22561 tttcaaggtc cacggggctt tgcctctgaa aatcatctgg tcgatgccgg tataaagcga
    22621 gccgaggtaa acgccccatt tgaatgacgg acggacgttg cgcgcggcgt aaagctcttg
    22681 atacagccag ctttgttcaa aacgttgctg ataatccgcc gcctctttgc cgctgtcgaa
    22741 accctccact tcttcaaggt tttccaacaa ggggaacacg gcttcggcgg cgagcatggc
    22801 ggatttcatc gcggtatgaa tgcctttgat gcgcggcata ttgaggaaac ccgccgcatc
    22861 gccgaccaaa atgccccctt tgaacgagag tttcggcagg ctttgcaaac cgccttcaat
    22921 cagcgaacgt gcgccgtaag caatgcggcg gccgccttca aaggttttgc ggatttcggg
    22981 atgggttttg aaacgttgga actcttcaaa cggcgacaga taaggatttt gatagtccaa
    23041 accgaccacg aagccgacgg cgactttgtt gtcatcgaaa tggtaaacaa acgcgccgcc
    23101 gtaggttttg ctgtccagcg gccagcctgc gctgtgcacc accaaaccgg gctgatgctg
    23161 ttcggacggc acttcccaaa cttctttaat gcccaagccg taagtttgcg gctggctgtt
    23221 ttggtcgagt ttgaagcgtt cgatgatttg tttggaaagc gaaccgcgac aaccttcggc
    23281 aaacagggtt tgctgcgccc aaagctccat gccgggttgg aatgaatcgg tcggctcgcc
    23341 gtctttgcca atgcccatat tgccggttgc aatgcctttg accgaaccgt cttcgtgata
    23401 cagcacttcg gcggcggcaa agcccggata gatttccacg cccatatttt ccgcctgctc
    23461 cgccaaccag cgcacgactt cgcccaagct gacgatgtag ttgccgtgat tgtcgaaatt
    23521 tggggtaata ggcagattga acgctttttt ctcagtcagg aacaacactt tgtcctgcgt
    23581 taccgtacgc gtcagcggcg cgcctttttc tttccagtcg ggaatcaact cattcagcgc
    23641 aatcggatcg ataaccgcgc ccgccagcga atgtgcaccc gcctccgaac ctttctccac
    23701 cacgcaaacg ctgatttcgc gcccgttctg ttcggcaagc tgcttgagtt tgatggcggc
    23761 ggacaaaccc gacgggcctg cgccgacaat cacgacatcg tattgcatac tgtcgcgggt
    23821 gatggattct gtcatggcgg ttcctgtgta tttattattg aattgcaaat ccgtaattat
    23881 acaacgggaa catatagtta ccaaatacaa caaaggtcgt ctgaaaacca tattttcggt
    23941 tttcagacga cctttgtcga aatttcaata agcacgccac cattttacct gtccgaccgc
    24001 aaactccgtc tgacgtttcg gactgcgtgt gaaaaacgcc ttatccccgc cggcatccct
    24061 ccctttcggc acaaccgcca aaatcttacc tgccaaattt ccctcacggg tttgccaagc
    24121 atccaaaaac tgcgccctgc tcatcgaaac atgacccagc gacgggtcgg caagcaaaac
    24181 cgtattgccg tctataccgc gcaataccga gaaatgatca tccttgcggt atttcagata
    24241 cacgatgacg gggatttgca actgtgcaag ctgctcgaaa gacagggcat agcctttcgc
    24301 ttcaaaaccc aaatcaggca taatgcgccg catatcctca aacgacgcgg gcatctgctc
    24361 cttatccagt ttttttaaca cgtcctcttc cgtcagcttt tgcccgtaaa aattgttcaa
    24421 aagcgtcgcc accgaagccg ccccgcagga aaaatccaaa tcctgcttta caatattgaa
    24481 atcgcgcctc tctttccaac tctgcacttt gatttttcca taagcaacag gattatagtg
    24541 gattaaattt aaaccagtac ggcgttgcct cgccttgccg tactatttgt actgtctgcg
    24601 gcttcgtcgc cttgtcctga tttttgttaa tccactatag gtttccgtgc ggacgtgttc
    24661 agattcccgc cttcgctgga ataacggcgg agcagtttct gcttttccga taaatgaccg
    24721 taacttaaaa tcccgtcatc cccacgaaag caaaaatcct gcctgtcgga tttcggtttt
    24781 tttgggtgtt tcgggaaact tataaatcgt cattcccgcg caggcgggaa tccggtttgc
    24841 tcggtttcgg tttttcgggc gtttcgggaa actgatgaat cgtcattccc gcgcaggcgg
    24901 gaatctagaa cgcgggacgg cggcaatatt caaaggttgt ctgaaaattc agaggttcta
    24961 gattcccact ttcgtgggaa tgacgggata taggtttccc tacggacgtg ttcagattcc
    25021 cactttcgcg ggaatgacgg cggagcgatt tctgcttttc cgataaatga ccgcaaccta
    25081 aaacccatcc ttcccgcaaa aacagaaaaa caaaaaccta aaatcccgtc atccccacga
    25141 taacagttgc gtaattgcgt agagtgggct tcagcccaac gttttttctt tttcggtcgt
    25201 tgattggtgg gctgaagccc acccttgtat atcggaactc ccgtatcata gcaacaaacc
    25261 gcccggccgc cacccgcgcc cacccaaggc acacaaccgt tgcgcagcac agggagcggc
    25321 agggcaaccc atcgacacaa ccggacagtt gccggacaac acaaccgaat gcaaggcagg
    25381 ttgatgatga gtacccgata ccattacgca ggtatagtga attaaatcta aaccagtaca
    25441 gcgttggttc gccctagctc aaagagaacg attctctaag gtgctgaagc accaagtgaa
    25501 tcggttccgt actatttgta ctgtctgcgg cttcgtcgct ttgtcctgat ttaaattttg
    25561 gggctgtact agattagccc taaattccac accaatcccg caggatttta agctgttgag
    25621 acggtgtgcc gaagttaaat cgaaattcgc attctttcaa gaacagcggg aaagatttac
    25681 gatcgattcc gttgtatttt cgcaagacgc gttttgcctg attccaaaag ttctcaatgc
    25741 cgttaatgtg gttctgacgg tctgcacact ccttggaatg gttgatgcgg taatggataa
    25801 aaccgctcac gtccaacttg tcgcagctgc tcagactatc ggtataaaca atactgtccg
    25861 gcatgatttt ctttttgatg acagggagta acgtttcaga cttggcattg tttacgacaa
    25921 cggtatagac ccgtccgttg cgtttcagaa tgccgaagac aaccactttt cctgccgccc
    25981 gcgaccacgt ctgcctttac gccgtccgcc gaaatcgctt tcgtccggct cgacagggcc
    26041 ctcaaaaacc tcatcggcag ccaaggccaa atgatggttg ataaccgtgc ggattttacg
    26101 gtagaacagt gctgccgaat tgggatggat acccaaaata tcggcggcag aacgggcggt
    26161 aatttccagc gcaaaaaaac ggagcagttc tttctgtact tttttcttta atttgcagtg
    26221 tgttatcttc atatttcgag ggtaacatat ctgctaatct agtacagccc caaaaatata
    26281 ccaaaaacag caaaacaaat tgtaaggata ggtataggct ttgtaaaggt aaattgtgaa
    26341 aaaagcagtt ttttaaacga atgaaacggc ttcgggctga aatatatgct gatgccctgt
    26401 ccttcccgta tatcttgtgt gttgtcaaag tgcaggctgc tttgaaattg gcattacagt
    26461 cgatgaacca ccattttgct ttatttcagc gggcttgaga tgtgtataag aatattgttt
    26521 tgaataaatt taaagaaaat gataatcgtt attgacgatt tttaaaggaa agcgtagagt
    26581 gccaattcta tgaagcaata cggtaagtaa caatgaaaat atctactgct tgggtataga
    26641 gcatatttca caacccgtaa ctattcttgc ggaaacagag aaaaaagttt ctcttctatc
    26701 ttggataaat atatttaccc tcagtttagt taagtattgg aatttatacc taagtagtaa
    26761 aagttagtaa attattttta actaaagagt tagtatctac cataatatat tctttaacta
    26821 atttcta
//
LOCUS       DAXHTY010000018        24914 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXHTY010000018 DAXHTY010000000
VERSION     DAXHTY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24914
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.018"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(93..398)
                     /locus_tag="ACFNMK_000655"
                     /pseudo
     CDS             complement(93..398)
                     /locus_tag="ACFNMK_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     repeat_region   838..1598
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=904..939
                     /rpt_unit_seq="gttgtagctcccattctcatttcgcagtgctacaat"
     gene            complement(1662..1988)
                     /gene="cas2"
                     /locus_tag="ACFNMK_000656"
     CDS             complement(1662..1988)
                     /gene="cas2"
                     /locus_tag="ACFNMK_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFC6363975.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     gene            complement(1981..2895)
                     /gene="cas1"
                     /locus_tag="ACFNMK_000657"
     CDS             complement(1981..2895)
                     /gene="cas1"
                     /locus_tag="ACFNMK_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HFC6363976.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKTELTLGIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            complement(2962..6211)
                     /gene="cas9"
                     /locus_tag="ACFNMK_000658"
                     /gene_synonym="csn1"
                     /pseudo
     CDS             complement(2962..6211)
                     /gene="cas9"
                     /locus_tag="ACFNMK_000658"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes.;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
     gene            complement(6489..9923)
                     /gene="dnaE"
                     /locus_tag="ACFNMK_000659"
     CDS             complement(6489..9923)
                     /gene="dnaE"
                     /locus_tag="ACFNMK_000659"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC6363977.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNIHHAELNPEWLANGDNSGLICLNGAHYGEVGVNLLNGNEDAARTAALKY
                     AAWFPDAFYMELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPDQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVRDKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLVDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVQLLA
                     AHQLPDTPRIPLQLSYANGKASGRFQVPPKWTVTPSSALFDELEKLLGSRSVRVNW"
     gene            complement(10142..10270)
                     /locus_tag="ACFNMK_000660"
     CDS             complement(10142..10270)
                     /locus_tag="ACFNMK_000660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363978.1"
                     /translation="MTLMHILKHELPDTLVIGISHQNEIKNLFERKINLTACHPSE"
     gene            10469..10702
                     /locus_tag="ACFNMK_000661"
     CDS             10469..10702
                     /locus_tag="ACFNMK_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6363979.1"
                     /translation="MEAIVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            10680..10883
                     /locus_tag="ACFNMK_000662"
     CDS             10680..10883
                     /locus_tag="ACFNMK_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363980.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGDACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            11328..12164
                     /locus_tag="ACFNMK_000663"
     CDS             11328..12164
                     /locus_tag="ACFNMK_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC6363981.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RY"
     gene            complement(12216..13508)
                     /locus_tag="ACFNMK_000664"
     CDS             complement(12216..13508)
                     /locus_tag="ACFNMK_000664"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC6363982.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALAAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKEGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIATPLLESGEMKRLADNVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(13566..15476)
                     /gene="pglD"
                     /locus_tag="ACFNMK_000665"
     CDS             complement(13566..15476)
                     /gene="pglD"
                     /locus_tag="ACFNMK_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HFC6363983.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTALLTIGLFVRMGLYRAVTRFVSFHVLTTAFAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRCPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVRTFVLISTDKAVRPTNTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            complement(15524..16699)
                     /locus_tag="ACFNMK_000666"
     CDS             complement(15524..16699)
                     /locus_tag="ACFNMK_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC6363984.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPTTKAVIVVHLAGMPAEMDGIMALAKEHNLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNGKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKSFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            complement(16777..18018)
                     /locus_tag="ACFNMK_000667"
     CDS             complement(16777..18018)
                     /locus_tag="ACFNMK_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFC6363985.1"
                     /translation="MNKFFKRLFDIVASASGLIILSPVFLILVYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMIKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMGYLPLYNEFQNRRHEMKPGVTGWAQVNGRNALSWDEKFACDIWYI
                     DHFSFWLDMKILFLTVKKVFIKEGISAEGEATMPPFTGNCKLAVIGAGGHGKVVAELA
                     AALGTYGEIVFLDDRAQGSINGFPVIGTTLLLENSLSPEQFDIAVAVGNNRIRRQIAE
                     KAAALGFALPVLIHPDAYVSPSATVGQGSVVMAKAAVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTHIGEESWIGTGACSRQQIRIGSRATIGAGAVIVCDIPDG
                     MTVAGNPAKPLAGKNTETLRS"
     gene            complement(18011..19183)
                     /locus_tag="ACFNMK_000668"
                     /pseudo
     CDS             complement(18011..19183)
                     /locus_tag="ACFNMK_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221486.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(19197..20268)
                     /locus_tag="ACFNMK_000669"
                     /pseudo
     CDS             complement(19197..20268)
                     /locus_tag="ACFNMK_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            complement(20265..21686)
                     /locus_tag="ACFNMK_000670"
     CDS             complement(20265..21686)
                     /locus_tag="ACFNMK_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC6363986.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVSKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANTPPARLSATAESASALLAAAVCLIGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAVYLAG
                     CILRHWKDLHKLFHYLKKQGFPL"
     gene            complement(21881..22990)
                     /gene="ribD"
                     /locus_tag="ACFNMK_000671"
     CDS             complement(21881..22990)
                     /gene="ribD"
                     /locus_tag="ACFNMK_000671"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC6363987.1"
                     /translation="MFSDTDISMMETALRLAALGRFSTSPNPRVGCVIAHGSQVVGQG
                     FHVKAGEPHAEVHALHQAGEMAQGATAFVTLEPCSHYGRTPPCAEALVRAGVSRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLRPYRKHAHVRILMPSETAGGK
                     IDLNSLMRLLADEGIGELIVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLFSLP
                     ENRAALSAPPLWMPVSTEILGHDIKTVFRKNGNAF"
     gene            complement(23023..23487)
                     /gene="nrdR"
                     /locus_tag="ACFNMK_000672"
     CDS             complement(23023..23487)
                     /gene="nrdR"
                     /locus_tag="ACFNMK_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC6363988.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPSCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTDRKA"
     gene            complement(23516..24358)
                     /locus_tag="ACFNMK_000673"
     CDS             complement(23516..24358)
                     /locus_tag="ACFNMK_000673"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC6363989.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     NDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT         5244 a         6191 c         7286 g         6193 t
ORIGIN      
        1 aaccgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgaga
       61 caacgccgta ctggtttttg ttaatccact atactgtaat cagggatgct cagttcgtca
      121 aagcggcaaa acaggttgaa atcgatgcgg gtgatgaggc tgtgttcgag ttcgggatcg
      181 gagaggctgt gccattgtcc gagcaggacg gctttgaaca tggatagcag tggataggca
      241 ggacggccgc ggtggtctct aaggtaacgg gttctttgac gattcaggta ttgttcgatc
      301 ggctgccaat caatcacctg gtccaacttc aatagcggga agcggtcgat gtgtttggca
      361 atcatggctt gggcggtttg ctgaaagaag atgctcatga gaaatcccct aaatgtcttg
      421 gtgggaattt aggggatttt ggggaatttt gcaaaggtct cattttgcaa gattttagtt
      481 taatcatcaa acagggaaca gcaaaaatcc ccgctgcaac ggggattttt gttcggctct
      541 cgcctgtgtg attaacttca ctaggagaag ctaaaatgct taaattcctt atgcgtggga
      601 aaaagttgct gctgtccgtt gaagtgaacc acaagttcat tttagcggtt gctttgctgt
      661 taagtcaatg cagtagctaa cccgaccaca atcaaaaaaa cgcaagtttt ccgcctactc
      721 tgtgagtggg cggttttttc ttatttcaaa tctgtgacac aatatctgtc gcctttcttg
      781 ccataattgc actgtgtctc aatgacttta acgcacgttc gcttatcgca acggctggtt
      841 gtagcgccca ttctcatttc gcagtgctac aatggacggc ggttctaaaa tatgaaaact
      901 caagttgtag ctcccattct catttcgcag tgctacaatg gtcagattat aataacgttg
      961 agcgcgtgtg ttgtagctcc cattctcatt tcgcagtgct acaatctcaa cattgttgta
     1021 atctgaccgc aacgcgttgt agctcccatt ctcatttcgc agtgctacaa tactccctgt
     1081 ttatcggttt ttcagcttaa agttgtagct cccattctca tttcgcagtg ctacaatgtc
     1141 aagcatacta aacgattaat agcttaggtt gtagctccca ttctcatttc gcagtgctac
     1201 aatacaaagc cgcaaatcgt ccgccgcgta tcagttgtag ctcccattct catttcgcag
     1261 tgctacaatc gacaacaaag ccgtctatga cctgaccgag ttgtagctcc ctttctcatt
     1321 tcgcagtgct acaatcaccc tgatattccg caactcgata tactggttgt agctcccttt
     1381 ctcatttcgc agtgctacaa tgtaaagtcg gcacaaagtc ttcaggtttc agttgtagct
     1441 ccctttctca tttcgcagtg ctacaatcat cgcgctgaac ataccggcgc agccgcagtt
     1501 gtagctccct ttctcatttc gcagtgctac aatcttccgg tatgacatca cgaagcgggg
     1561 cggttgtagc tccctttctc atttcgcagt gctacaatac ttcgacggga aatccttatt
     1621 tcataagggt ttcccgtttt atttgttatg aaaaaatggg cttaaaataa caataattgg
     1681 tctgaattga cctttttttc ttgggttttc agctcgccca acagcagttt catggcggca
     1741 tattgctttt ctgttatctc caaacagcgg attgagcctt cttgcggaag gtttgcacac
     1801 agtctgttgt ggtgtttttg caacgaatcg cggcctttga cgatacggct gtatacggaa
     1861 agttgcagca tttggtatcc gtcttttaat aaaaactggc ggaattgatt ggcggctttg
     1921 cgctttgccg ccgtgataac cggtaggtcg aagaagacga taatcctcat aaatttggcc
     1981 tcactcatat tggtattctt tcaatggtag gatttcaggc agtttcagtt gtttggcgtt
     2041 tttatcggtt acgccggctt gaaatgacga aatcattttg tcgatggcag ccaaggtgct
     2101 gaaatgttgc cgttcgatgc tggtttgata gtagaggatt ttgatgaggt gttgtttgat
     2161 gccgagcgtc agttcagttt tcagacggcc ttgttcgtac aaatgtatca cggtcaggtc
     2221 ggccaatggg cgtaagggtt cgataaagtc atcggcgaga ttaaatggat tgagttcact
     2281 tctatgaaac aagcctaatg caggtaacca accgtataag gtcagggcgc gtgcaactgc
     2341 ggcacggagt acggcgtagg tgtagttcaa tgcggcattg acggcgttgt tgtcattacg
     2401 ggtaaatttt tctccaaaga gggcttgaaa gtagagggcg gcagcttggg cttcacggtt
     2461 tcctgtatcg cctgaacgga cttcagatgc caaagtgcgc agccgtttgg ctgcaagatc
     2521 attgcctgtt tcgtctgcga caaatgcttg gttcaggatt ttttggcgga cgatatgttg
     2581 ccagagttgc ttttttaagg gttcgcttat gttcagttgc agtttgagga ttttaagttg
     2641 gcggtgatat tgggcataag gcagccattg tccgcagggc aggaactgct cgtcgcaggt
     2701 cagcagggtt gcaccatgct cggctaaggc tgataggagc ggggcggtaa tcagggtttc
     2761 gcgattttcg atgatgataa cggcgatgtc ctctaatggt acggtgtggg actcgccgtt
     2821 ttgttgaatc agcagttgcc ggcgttgcaa ggatagtttg ccgccgtttt gaataagcag
     2881 gctgcgccaa gtcatgtttt tccccttatt tttatataaa aaatccctgc ttcaggccgc
     2941 ctgaaacagg gatagattaa tttaacggac aggcgggcgt tttttcagac ggcatggtct
     3001 gatttctttg cccagttcgt caatttggta tttttggaat gaaagggcgg ttttgacgcc
     3061 gataccttcc agtattccat ttttgccaat tttatgatca agatcatgaa tgcgtatatt
     3121 gatattacct gtgcctcgat ggcagctggc aaagtaacca aacattctag ctttttttgt
     3181 tataacctcg actaaatcat tagggtgtaa tgagaattta aagttgaaac tatcatcaat
     3241 aagttgccaa tcttcttcat cttttccttg tacaacagcc ctatccggcg aaaatccctt
     3301 tcgctacctg ccaactgtaa atcggtacca gataatactt gtcgcctttc tcaaacacat
     3361 ctacgcgcac catggttgcg ttgtcggcaa taccgttatg gttgcgcacc catacgccgg
     3421 ttttctgtac ttgctctacg cgtacggctt ttacctgttg ggtgcggttg cctgctttat
     3481 cgtatttgta aaacggctcg gcaaaggctt tggcaggatc gtctttatgt gcttccagcc
     3541 gtgctttcag tgcttcgtat agcttaggtt cgcgctcccg attgaccatt ttttccaagt
     3601 ctttcagttt taactgtgtt aacggtacgc gcaacacgct gacgccttcg tccagtcgtt
     3661 tggcggattt gacggtctcc atatgccctt gcccgctcat cttccgattg ggcgcgcgtg
     3721 aaacaaacag tggcgtaacg tattcgtgta cggcttcggg gcgagatgat aatttttcgg
     3781 caagcaacgt gcgcagtttt tctggggtat cggcttcttc gaattcgggt ttgccgtccg
     3841 gtttgccgaa gacgcgaatc atgacttctt gtgcgaaaaa ttcccaaggt tgtgggaagt
     3901 gtgttttttg atgcagcact tctcctgttt ctttgtctat ggttttaccg tcaaacgcgt
     3961 tcatctcttt atagcgtaca aaacgggtaa ttttctgctg catggcaacg gtcgagcagg
     4021 caacgacgac ggcgtccaag gcgtgatggc ggtcgttttc cgcacgcact ttgcgcaatc
     4081 cccaaaagcc gcgcaacaga ttggtaattt gtccgttgga tgcaaagaca cgtttcttgc
     4141 ctttacctgt cagccgcata cggtcggcaa caaattgaca caggaaacgg ttgacgtagc
     4201 gcgtgtcgtt cagattgcgt tctttaaagc cgtcttcatc gaatttttgc agcagaatcc
     4261 gttgtttttt actgcgcggg aaacggctgg tttcgacacg cgctttaaat tcctgccatt
     4321 cgcggctgtt gtctttgccg ttgaagtatt cgtaaggggt ttgattgcct ttgttttggt
     4381 tttcgctgcc caataccagt actttattgt tgaaactgtc gtcccatgtg cgcgagaacg
     4441 gcagggcatg gtcgatttcg acatagcctt tttcgttcag acggcctaag ttgatttctt
     4501 tgcccgaata caggcatttg ccgtgttgtt gctcgtacag gcgcagtttc agaatatctt
     4561 tggatttggg ttctccgaca aaattgggga aatactctcg gaatttggcg gcggcttttt
     4621 ccctgtcttt gcggttttct tcttggcgtt tctcaatttc tttgcggtct ttaaacgatt
     4681 tacctacttc ccttgcagtt tcaatatgga tacgagctgg ggagccgtaa cggcgtacca
     4741 cgccgttaat gaccttacgt gcttgagata aggcgcgcaa gacgacgggg ttgcggattt
     4801 cgtcggcggg aatcggcggc agataaatct tttcttccgt attcttcttg ccgtaatggt
     4861 ctccgtagat ttcggcgcag gcttcatcgt aacgtttgcc ttgttccatt agaggcacaa
     4921 ttcggcgcaa tgctttcaag gaaatttgga cgaacttatc gaagctgatg tgtttcaaca
     4981 gcgcttctaa gatttcgggc tgtatacggt ctttcagacg gcctgtaatg tcttcatcgg
     5041 ttttgaacag ggagaatgcc gtgccgattt cgtcttgtaa ttcgggagaa aggtttaatg
     5101 gggatttttt gtctttcaat ccttcttttt ccagtgcacg gctgatggca tggtaggcct
     5161 tcatttccat caatgttgag gcttcggcat tgtctttacc atagcgcaag cctttgaaaa
     5221 aggcggtatc ttctaaaccc agcagcttac gggcttgtgc gtaagtcagt ttggattttc
     5281 tgtatggctc gtccataagc gtggcgcgtt cggtatcggt caatggccgc tcgctgcctt
     5341 gctctaaaat acgcaggttg ttcagcttgg tcagccagat gaaacgttcg gctgtgtagg
     5401 tgtttttagc ggctttcggc tctgccggtt cgaaggtgca atgccccaac attttttgaa
     5461 cggcatcgcc ggacagggca gggcgttgcg tcatcagtag ggtttcaata ccttctttaa
     5521 ggccgcctga aacatgcgga ttgccaaatt ctttttgttt ttcaaacagc aaaatcagct
     5581 ccgcctgtaa atctttgcgg ctgaacgtat gcgaataatc gccgcgctga ttgcggatat
     5641 ggccgctttc tttctcaaat ttatttaaag ccaattcggc cggtgtgcgg aaatcgcctg
     5701 tctgtaaggc atgggcattg tcggctacgc ctttgagcaa agcgccaagc tctttatcgg
     5761 cagtttcgcc ctcgtttttc cgttgcgata aatagccgcg atgtttgatt aaatgcaaca
     5821 agactgccga ccactctaaa ggcgtcagtt tgcggtctaa tgcggctgcg cgaagttgcc
     5881 atggtgtatt cggtaaggat ttgatcaagc cgttttcgtc aaaatcagcg gcttgtaata
     5941 cgccttcgcg ttttaatagg cgacgagctc gaagcaggcg gtgggcgcga cggcgggtca
     6001 ggcggcggac gctgcgtgcc aagcgtcttg ctgctgcgag agagtcacct gttttcggta
     6061 cttctgcgcg ctcaaatacg cgcacgccta aatcaatcag gcagattggg ttttcatctt
     6121 cgtcaatttc taccatcgcc cagccgacgg atgcgatgcc gatatcgagg ccgaggatgt
     6181 agttgattgg attaggtttg aaggcagcca tttattactc cgtaaacaac gttgcaaata
     6241 atgataatat atcttattgt cttatttata tacaaataga ttattgactt atcatctcac
     6301 aacggctagt atcccaaaca tattgtcgca ctgcgaaatg agaaccgttg ctacaataag
     6361 gccgtctgaa aagatgtgcc gcaacgctct gccccttaaa gcttctgctt taaggggcat
     6421 cgtttatttc ggttaaaaaa tgccgtctga aaccgatttt gggcttcaga cggcatttat
     6481 attttgggtt accaattcac gcgcaccgac cggctgccga gcagtttttc cagttcgtcg
     6541 aacaatgcgg aactcggtgt aaccgtccat ttcggcggca cttgaaacct gcccgacgct
     6601 tttccgttgg catacgacag ttgcagcggg atgcgcggcg tgtcgggcag ttggtgggcg
     6661 gcaagcagtt gtaccagtcc gccgatgtcg tgatgcgggg cgagggcgag gctgaggctg
     6721 cgggcgtagc gttcgcgcgc cgtttgcagg gtcatgactt ggtttgccag aatacgcagc
     6781 ccgtcgccgc cgccgtagtc gtcgcggctg actttggatt cgataatcag cacttggtcg
     6841 gctttgaggc agtcggcgca gttttccagc gtctgaccgc cgaccatgat ttcaacctgc
     6901 ccgctcaaat cttcaaggct gacgaaggcg attttgccgc gcttgcccat catcgtacgc
     6961 acggcggtaa cgaatccggc gaggcgcacg ctgtcttgcg gcttcagacg gtctaatttg
     7021 gtcggtgcga tttggcggac ttcttgggca tacgggccga acgggtggcc ggagaggtaa
     7081 aagccgatga cggttttttc ttcggcgagt ttttccgatt cgctccacat gggtgcatcg
     7141 acgagccgca ccggttcgat ggcgtcttcc atcatatcga acagcccgcc ctgattggcg
     7201 ttggcggctt tttggtcggc gttgtccata gcgaggtcga tgtttgccaa gagcatggcg
     7261 cggttgggtt cgatgctgtc gaacgcgccg ccgcgtatca gggcttcgag ggtgcggcgg
     7321 ttcatgtgtt ctttgccgac gcgctcgcag aagtccaaga ggccggtaaa tttgccgccg
     7381 ctttgccgcg cggcgatgat ggattcgacg gcggcttcgc ccgtgccttt aatcgcgccg
     7441 agcgcgtagc ggattttcat gttcggatac ggcgtgaagc ggtagtctga ttcgttgatg
     7501 tcgggcggca ggaactcgat gccgttggca cggcagtcgt cgtagaaatg cttgagctgg
     7561 tcggtgttgt ccaattcgga cgacatggtc gccgccataa attcggcggg gtagtgggct
     7621 ttgagccatg cggtctggta ggaaatcagg gcgtaggcgg cggcgtggga tttgttgaaa
     7681 ccgtagccgg cgaatttttc catatagttg aagatttcgt cggatttttc gcgcgaaatg
     7741 ccttgtttcg ccgcgccttc ggcgaagatt tcgcggtgtt tcaccatttc ttcgggtttt
     7801 ttcttaccca tggcgcggcg cagcaagtcc gcgccgccga gcgagtagcc gccgataatt
     7861 tgcgccgcct gcattacttg ttcctgatac accataatcc cgtaagtcgg cgcgaggatg
     7921 ccttcgagca gcgggtggat gtattggaat tcctggcctt tcatgcgcgc gacgaagtcg
     7981 ggaatattgt ccatcgggcc gggtcggtag agcgatacga aggcgatgag ttcttcaaac
     8041 ttggtggtgt gcgccgtttt cagcattttt ttcatgccgg tcgactcgaa ctggaagacg
     8101 gcggtggtgt tcgcgtcgcg gaagatttgg taggcggcct ggtcgtcaag cgggattttg
     8161 ccgacatcga tgatgtcgcc ggtggtgttt ttgatgttgt tctgtgccat ttcgataatg
     8221 gtcaggttgc gcagacctaa aaagtcgaat ttcaccaaac ccacgtcttc cacgtcgccc
     8281 ttgtcgtaca tggatacggg agaggcggat tcatccgcct gatacacggg gctgtaatcg
     8341 gaaatcttgc ccggcgcaat caacacgccg cctgcgtgca tacccaaacc gcgcgtcagg
     8401 tcttccagct ttttcgccag cgtaatcagt tcgtccgctt cttccgcttc gattaattcc
     8461 tgaatctgcg gctccgcctt catcgcgtcg tccaaactca ggggtttgtt ggcttccaac
     8521 ggaatcagct tggacagttt gtcgcacagc ataaacggca gctccaacac gcgccccacg
     8581 tcgcggatga ccgctttgga cgacatcgtg ccgaaggtaa caatctggct gaccgcctga
     8641 gcgccgtatt tatcgcgcac atattcgatc acgcggccgc ggtttgcctg acagaagtcc
     8701 acgtcaaagt cgggcatgga gacgcgttca gggtttaaga aacgttcaaa cagcagcgcg
     8761 tatttgagcg gatcaaggtc ggtaattttc agcgaatacg ccaccagcga acccgcgccc
     8821 gaaccgcgtc ccggcccgac cggacagccg tgcgttttcg cccagttgat aaagtcttgt
     8881 acgataagga aatagccggg gaatttcatc tggatgatga tgttcagctc aaaatccaaa
     8941 cgttcctgat attccggcat ttttgccgca cgctccgcct cgtcgggata aagctgaacc
     9001 atgcgttcct gcaaaccctc gtttgagagt ttgatgagat agtcatcgcg cgataaaccg
     9061 tcgggcgtgg ggaaaagggg caggaagttt ttgcccaatg tgatgtgcag gttgcagcgt
     9121 ttggcaattt ccaccgtgtt ttccaaggcc tcgggcaaat cggcaaaacg ctccagcatc
     9181 gtttccggcg gaatgaaaaa ctggtccggc gtgaaatcgc gcggacgttt cttgtccgtc
     9241 agcacccagc cgcctgcgat gcatacccgc gcctcgtgcg cgttgaaatc gtcgcggctc
     9301 ataaactgtg tcggatgcgt cgccaccacc ggcaaaccca attcctccgc cagcttcacg
     9361 ctgcccgaaa cgcaagcctc ccattcgggg cgttcgggca ggcgttgcag ctccatatag
     9421 aacgcatcgg ggaaccacgc cgcatacttc aacgccgccg tacgcgccgc gtcttcattg
     9481 ccgttcaaca gattcacgcc cacttcgccg taatgcgcgc cgttcaaaca aatcaagccg
     9541 ctgttgtcgc cgtttgccag ccattcggga ttgagttccg catgatggat attgcggtct
     9601 ttgccgacat aggccgccgt taaaagctcg ctcaagcgca gatagcccgc atcgttgcgg
     9661 ataatcagca tggcgcgaaa cggcttgtcg ggcgcattcg gattgcctat ccatacgtcc
     9721 gccgcgccga cgggcttaat ccccgcgctg cggcaggctt tatagaattt caccaagccg
     9781 aattcgttca tcaaatcgct gatgcccaaa gcaggcaaac cgtattcctg cgctttggca
     9841 atcagttttt taatccgcac cataccgtcg gtaatcgaaa actcggtatg caggcgcagg
     9901 gggatgtagg tcggctcggt catggcaaaa tcggcgtgaa caataaaagg cgtattgtag
     9961 cagggttgtc tttagatggc ggtgtaggtc atgccgcttc ggattcagac ggcatgacct
    10021 gcaaatgttt ttgagctttt acgacggcaa aaaaaatgcc tcctgccgta tggcggaggc
    10081 ttcccaagga gtattgatag atataaagga ctatcaaact agttataaag aactatatac
    10141 cttattcgga cggatggcaa gcagttaaat taattttacg ttcaaacagg tttttgattt
    10201 cgttttgatg gctgatgccg atgaccagtg tatcgggcag ttcgtgtttg aggatgtgca
    10261 tcagcgtcaa tgcggattcg tcggggaaca ggatttgcag tctgccgttg gacaggattt
    10321 ctttgatcgg atggcggcat atggggtttc agacggcatt tttactttta cggaacaggc
    10381 ggtgcggata catcgctggt tttgcggtaa ttttgggtac ggcggcggca ggtgctacaa
    10441 taacggtctc tttctgcaag gggacatcat ggaagccatc gtctatctcg aagacaacga
    10501 atacatcgcc ctgtgcgacc tcttgaaatt ggccggactt gccgaaagcg gcggacaggc
    10561 gaaggcgttg attgccgaag ggttggtgtc gcgcaacggc gaaaccgaaa cccgtaaaac
    10621 cgccaaaata cgcggcggcg aagtcatcga gtttgacggc gcgcgcttgg aaatcgccga
    10681 tggatacgac cctgaagcat aaagccgaag cccttttggg cgagccgctt ttagacgaac
    10741 ccgtccgccc cgagtcgtgg gaatgctgcg gcagcgactg cggcgacgcg tgcattcaga
    10801 cgatttactg ggcggacaag gcccgctacg acgcgcaacg gcggaaattg aaagaagcgg
    10861 gttggtcgga cgatgccgtc tgaaaacggt tcggcttgat atagtgaatt aacaaaaatc
    10921 aggacaaggc ggcgagccgc agacagtaca aatagtacgg aaccgattca cttggtgctt
    10981 cagcacctta gagaatcgtt ctctttgagc caaggcgagc caacgctgta ccggtttaaa
    11041 tttaattcac tataaaacca cgttttcaga cagtctttta tagtagatta aaattgaaaa
    11101 catccctatc gtcattcccg cgcaggcggg aatccggaag tttgaaatag cggttaacct
    11161 taaacatttc caataaatca aagtctggat tcccgcctgc gcgggaatga cggtataggg
    11221 actttttctt tgcatttgcc atacacctgt tttttaaaat gaccgccgcc gccacactgc
    11281 ccgacaatat tgcccccgac cttcattcac cacacatata acaccccatg atagacctgc
    11341 actgccattc caccgtttcc gacggtatgc tttcccccgc cgaagtcgtg cgcctcgcgc
    11401 atcaaaacgg ctgcacgctg ctggcgttga ccgaccacga ccacaccggc ggcatcgccg
    11461 aagcgcgtgc cgaagccgac aggctcggtt tgcgcctgat taacggcgtg gaaatctccg
    11521 taacgtggcg cgggcgtacc atacacgttg tcggcttaga ctttgacgaa caagacgaaa
    11581 acctgcaaaa cctgttggcg caagtgcggc aaggccgtct gaaacgcctc gaagccatcg
    11641 ccgccaagct ggaaaaacgc ggcatttcgg gcgcatacga cggcgcgctc gcgctggcgg
    11701 caaacaaaga aatggtcagc cgcacacacg tcgccgagtt cctcattcgg gtgggacacg
    11761 tcaaaaacaa gcagcaggcg ttcaccaaat acttgggcga cggcaaatcc tgcgccgtcc
    11821 gccacgaatg ggcgacgctg gaagactgcg tctccgccgt caacggcgcg ggcgggttgg
    11881 cggtcatcgc ccatccgatg cgttatgatt tgtccgccac cgccaagcgc aatctgtttg
    11941 aagaatttaa aaacctcggc ggcgcaggca tcgaagtcca cagcggcaac tgctgcaaaa
    12001 acgaccgcct caactacgcg cttttggcag aacgcttcgg tatgctcgcc agcgcgggca
    12061 gcgacttcca ccgtttaaac gactttagcg gcggcattct cggcgcgtgt cccgaactgc
    12121 cggaaaactg caggccggtg tgggcgcatt tttcccgata ttgaatgcag ataaaaatgc
    12181 cgtctgaaaa cctttcagac ggcatttttt gcggtttaaa cgttgtcgta cagttttcgg
    12241 acggttttac cgatggcggc aatgcctttt tccagcgttt gagcgtcctg cgcgatgctc
    12301 atgcggatgc actcgcccgc gtgcgggtaa tcccgcgtgt cgatgccgac gaagaaatgc
    12361 tcgcccggaa taatcagcgt accttcggct ttgagcattt cgtacagggt ttgcgaagaa
    12421 acgggcaggt tttcaaacca gagccacagg aaaatcgcgc cttcgggttt gtggattttc
    12481 atcgggtacg cgcccagctc gcgcttgagc agcgagacgg cggtttgcgc ctgattgcgg
    12541 taaaacggcc ggatgacgtt gtcggcaagc cgtttcatct cgccgctttc cagcagcggc
    12601 gttgcgatgg ccgcgccgaa gcgcgtgggg gcaaggttca caatcgcgtt caggctgctg
    12661 acggctttga ccacttccgg cgcggcgacg atgatgccgg tgcgaacgcc cggcaggccg
    12721 actttggaaa ggctgaagca gaggatgatg ttttcatgcc aattcagcgt tacgtcgctg
    12781 taaatgatgt tggggaacgg cattccgtag gcgttgtcga taatcagcgg aatcccgtgt
    12841 tcacgcgcca aagcgtccaa acgcgccatt tcgccgtcgg tcaacacatt gccggtcggg
    12901 ttggtcgggc gcgaacagca aatcgcgccg attttgcctt ctttcagttc gggcaggttt
    12961 tccagtgcgt caaagtccac gcggtatttg aagaagcctg cttcgccttc gtgttcgacg
    13021 ttttcgattt tgggcttgac ggaaacgaag tgctgccctt cgacatgcac gtcggcatag
    13081 ccgatgtatt cgggcgcgag cggcaacaaa atggcttttt ctgcggatgt gccgtctgaa
    13141 aggttgaatt tgccgccgaa gaggttgaat aaatagaaaa acgcgttttg cgaaccgttg
    13201 gtcagcgcga tattgtcggc ggtcaggttc cagccgtatt cgcggttgag gaaggcggcc
    13261 agcgcgtcaa tcagcgcggc atcgccttgg gggttggagt agttgccgat gttttcgacg
    13321 gcatgttctg ccgccagttt ggagaatatg tcggcaaacg cccgatcgat ttccggaatg
    13381 cgcgccgggt tgccgccgcc gagcatgttg acgggcttgt cgcttttgag cgcgtcgccg
    13441 aggtcgtcca tcagttgcag gatgccgctg tgttgcgtga atttttcgcc gaatgctgaa
    13501 aactgcatat caaactccgt gtgtgtgtca ggcaggttgg tttgtacgtt atgccgtctg
    13561 aaggttcaga cggcattttt tctgtgtgtt tctcgtaccc aaagcaggtc gcagataccg
    13621 tcgcttgggg taaagccggt cggggcgttg atgagcaggg tgcggattgc ctgctggtcg
    13681 taacggtcgc aggccgcacg gatgcggtcg agcaggacag agagctcgtg ccacggcagc
    13741 atcgcctcgt cagccgtcat aatgcgcgga tggccggttt tgcggacgtt gtcgccgatg
    13801 agcagctctt cgtagagttt ttcgccggga cgcagtccgg taatgaggat ttcgatgtcg
    13861 ccgtcgggtt gttcgggtgt tttgggtttg aggccgctta aggtaatcat ttggcgggca
    13921 aggtcgatga ttttgacgga ttcgcccata tcgaggacga atacgtcgcc gccagtaccc
    13981 atcgcacctg cctgtatgac gagttgggcg gcttcgggta tggtcatgaa atagcgcgtg
    14041 atttcagggt gggtcagggt aagcgggccg ccttcggcaa tctgtttttc aaacagaggg
    14101 acaacggagc cggacgaacc taaaacattg ccgaaacgca ccatgctgaa gcgggttttt
    14161 tgtccgggtt cggcggcgag cgcttgaagg cagagttccg ccatgcgttt gccggcaccc
    14221 atggtgttgg tggggcggac ggctttgtcg gtggagatga ggacgaaggt tcttacgccc
    14281 gatgccgtgg cggcaagcgc gcactcgagt gtgccgaaga tgttgttgcg tatgccttcg
    14341 acggtgttga actcgaccat ggggacgtgt ttgtaggcag cggcgtgata gacggtcgca
    14401 acggaaaagg cggtcatgac gtgttcgagc agcgtgcggt tttgcaccga accgagaaag
    14461 ggcaggattt cggtgtcgag gcgtttttgg atgcaggttt cgcgcaattc tttttcgatg
    14521 gcgtacaggg cgaattcgga taactcgaac agcagcagtt tttcggggca gcgacggata
    14581 atctggcggc agagttccga accgatggag ccgcccgcgc cggttaccat gacggttttg
    14641 ccttcgatgt cggcactcat gaggcggttg tcgggcgcga cggaatcacg cccgagcagg
    14701 tcggacacag agattttttt gagcgtgccg atgctgattt ttccgtccat caggtctttc
    14761 attccgggaa tggtcaacac ttcgcacgga taggcttcca gtttgttgat gattcggcgg
    14821 cgttgttcct gggtcgcgcc ggggatggcg agcaggattt tttccacgcc gtagcgttcg
    14881 atgaggaagg cgatggcatc gggctggtaa acggcaaggt cgtagatgac ggtgtgccac
    14941 agtttggggt cgtcgtccac aaaggcggcg gcggaatatt cgcgcatttg tttgacggcc
    15001 tcaagcagtt gtctgcccga ccgtcccgcg ccgtaaatga tgacggggat catctgtttt
    15061 ttggggtgtt cggacaacag tccgcgcaaa accatacgcg agccggtcac ggaaacaaac
    15121 agcagtaaga aatagacaat cggcagggcg aggcgcagcc tttcttcaaa aatcaatgtg
    15181 ttgaggaaga acaggacggc ggaaacaatg ctgccggcaa aagcggtggt caggacgtgg
    15241 aaactgacaa agcgtgtaac ggcacggtag agccccattc tgacgaacag gccgatggtc
    15301 aacagcgcgg tcaataggaa agactgccag ttggcagggt cgaaccattc gttcgagtag
    15361 tcggccttca ggctttgggt aaaccaaaag gcaatgaaaa tcatcagaaa atcgtgtatg
    15421 aggaaacaga ttttcttgat gttgcgcggt agggcaagca gggtttccag agtcatatcg
    15481 tggggcggta tgtgctttca ggcggcatat gccgtctgaa gggttatcgt gtggcttcgg
    15541 tcaatacggc ttcgatgtgt tctttgcaga acgcgatttc gccgtcggtc agcgtcggat
    15601 gtactaagaa catcaggctg gtgtcgccca actcgacagc atttttcagc cgctctttcg
    15661 gacgccaagg cgtgttgtcg aaggatttct ccaaatacac ttcggagcag ctgccttgat
    15721 agcaggggac tttgcgcgcg ttcagttcgt tgacgatgcg gtcgcgcgtc cagccgtctt
    15781 tgaggtgttc gggtttgacg aaggcgtaga acttatattg cgcgtgtccg atgtagtcgg
    15841 cgacttcaac caagcggata cttttgaatt tgcctaggct ttccgccagc ttggcggcgt
    15901 gcgcttgacg gcaggcagtc cattcgggca ggcgtttgag ctgaatgcgc ccgattgccg
    15961 cctgcatttc catcatacgc cagtttgtgc cgaaactttc gtgcagccag cggaaaccgg
    16021 gcgcgtgttc gcggtggtac acggcatcgt agcttttgcc gtggtctttg tacgaccaca
    16081 ttttttccca cagggttttg ccgttggtcg taaccatacc gccttcgccg ccggtggtca
    16141 tgattttgtc ttggcagaac gaccacgcgc cgacgtgtcc gatagagccg acggatttgc
    16201 ctttgtattt tgcgccgtgc gcttgggcgc agtcttcgat tacccaaaga ttatgttctt
    16261 ttgccaaagc cataatgccg tccatttcgg cgggcatacc ggcgaggtgg acgacgatga
    16321 cggctttggt agtcggtgtc agcgcggctt tgacggtttc cgcgctgatg ttttggctgt
    16381 tcaaatccac atcggcaaac accggatttg cgcccgcgtt cacaatgcag gacgcggaag
    16441 cgaggaaggt gcgcgaggta acaatcacat cgtcgcccgc gcctatgccc attgctttga
    16501 gcgcgacatc gagtgccagc gtgccgttgg caagggcgac ggcgtaccgc gtgccggcaa
    16561 aggcggcaaa ttctttttca aattcgcggc attcgttgcc cgtccagtag ttgactttgt
    16621 tggacagcag gactttggaa acggcatcgg cttcttcttg ggtgaagcag ggccacgggg
    16681 aaagggaggt gttcagcatg gtgtttggtc cgtcggtttt cagacggcat ttcctcattt
    16741 ctgccgtctg ttgcttaggc tgtttatttt aatcgtttac gaccgcaggg tctcggtatt
    16801 tttgcctgcc aatggctttg ccggattgcc cgcgacggtc atgccgtccg ggatgtcgca
    16861 cacgataacc gcccccgctc caatggttgc gcggctgccg atacggattt gctggcggct
    16921 gcatgcgccc gtgcctatcc agctttcttc gccgatatgc gtgttgcccg acaggtgcgc
    16981 gcccgggctg atgtggacga aagcgtcaag caggcagtcg tgatcgacgg tggcggcggt
    17041 gttcacaatc acgccgtctt tcaatacgct gcctgcctgt acggcggctt tcgccatcac
    17101 gacgctgccc tgaccgacgg ttgccgacgg ggaaacatat gcgtccggat gaatcaggac
    17161 gggcagggcg aagccgagtg cggcggcttt ttcggcgatt tggcggcgga tgcggttgtt
    17221 gccgacggcg acggcgatgt cgaattgttc gggcgataaa ctgttttcaa gcagcagcgt
    17281 cgtgccgatg acggggaagc cgttgatgct accttgggcg cggtcgtcca aaaaaacgat
    17341 ttcgccgtat gtgccgagtg cggcggcaag ctcggcaacg actttgccgt gtccgcccgc
    17401 gccgataacg gcaagtttgc aattccccgt gaaagggggc atagtggctt ccccttccgc
    17461 cgaaatgcct tctttaataa agactttttt gactgtcaga aacaaaattt tcatatccaa
    17521 ccaaaagctg aagtggtcga tgtaccaaat atcgcaggcg aacttttcgt cccacgaaag
    17581 cgcgttgcgc ccgttgactt gcgcccagcc ggtcacgccc ggtttcattt cgtggcggcg
    17641 gttttgaaat tcgttgtaaa gcggcaggta gcccatcaaa agcggacgag ggccaactag
    17701 actcatgtcg ccttttaaga cattccataa ttcaggcagt tcgtccaaac tagcggcacg
    17761 cagttttttg ccgaagggtg tgagccgctc gctgtcgggc agcggaatgc cgtctgaatc
    17821 aagcgcgtcg cgcatggagc ggaatttaat cattttaaac ggcttgccat ccttgcccgg
    17881 ccgctcttgg atgaagaaca cgggcgagcc taggtttttg cggatgaggt acaccaaaat
    17941 caaaaacaca ggcgacagga taatcagtcc tgaagcggag gcgacaatgt caaacaggcg
    18001 tttgaaaaat ttattcatga gctaatcttt caatcaggtt gacgattttt tgataggcaa
    18061 cgtcgcgctt gaaacggcgg acgatttctt cggactgaac aggatcgttt ttgcgcttca
    18121 aaatatcttt ggcggcttga acgaaaccgt ccacatcgcc ggaacggtag ttcgcatacg
    18181 gcagcagggt aaggactttg gcaacttcgt cgttgaccta gctgttcaga atcggttttt
    18241 gcaaagccat atagtcggag agtttgttgg taatcgatgc cattgaatag gactggatgg
    18301 cgttgatggc aatgtcgcag cccttggcaa ccgacatcat ttcggcatag ggaatgtagc
    18361 cgtaaaattt gatgtcgtcg caggcgtatt gtttgagcct gtccaaatca gggccgccgc
    18421 ccatgatgtg caactcgacg ttttcgccgg cgtccaacag cttgagaacg cctttgcaca
    18481 cggtttccac gtcatagctg taactgagcg tgcccaagta gaaaaagcgg gttttgtcgt
    18541 cgccaaaatc tttggcaggc gcggcatcaa gtttgggaaa atccgcgccg atatagacga
    18601 cttcgccggg tacgttcgga ttggtttctt tggcgcggtc gagataagtc tgcgatacgg
    18661 caaccagcgc atcggcgtag cggtaggctt ggttggcacg tgaagcaaag ggcagcaggt
    18721 tgtgcggtac ttttttcaaa aacggtacga ctgaggagaa ggactccggc catacgtcct
    18781 gcacatcgac aatcagtttg tagcccaaac gtgctttgtg tttgcccaac agcaggttgg
    18841 tggcaatcag cggataggcg gaatagacga tgtcttgttc gcccggacgg cagttttcca
    18901 accatttttc aaaatgtctg acgaagcggt gatggctggc caagcgtcct aaagacacgt
    18961 ttttgccgta gccgctttct tccaacagca tgactttcag acggccttgt gaggcggcct
    19021 cggcatcttc gggccgtctg aaagatttgt cgtagtgttt gaagttgctg gtaatcagca
    19081 gcacgtcatg cgactgcgac aacagctcgg caagataccg aaagcggttg aagtgcggct
    19141 cggacggcag cgagcaataa ggggcgacga tggtaatgtt catgtttcag acggccttac
    19201 atttcgtacg cagccatggt ggtgcggtag atttcgtcgt cggagcataa ggtttcgaca
    19261 tgtttgtgca gggctttgcc catttggctg cgcaactctg gatgcttgat gagtgtatcg
    19321 acggcttcga taaatgcttc gtcgtcgccg aaaggaatgc agtagccggt ttggccggta
    19381 atgaccattt cggaaatgcc cgccatgttg taggtcacga caggcgtgtc gtaaaggccg
    19441 gcttctaaga tgttgttgcc cacgcctgcg ccgtggtcgc cgatgcagtg gggcgtgttc
    19501 accaaaatat ccacgtcttt gaagtaggcg gtcaaatcgc gcacgccgcc gaggaaggtg
    19561 actttgtctt caatgcccaa acgtttggct tgggctttga ggttgtccat ttcttcgccg
    19621 atacctgcaa cgttgaggcg tacaggcata ccgcggtcaa ccattttctt caaaatatcc
    19681 aacatcagat gcacggcgcg gacggtgtcc aaacgggaaa gtgtgccgag tacggcgcat
    19741 tcttttttgg ttttccggaa atgaaattcc cggggggggg agtgcgttgt aggcgtaggc
    19801 aatccggccg gcggggaagc cgtggcggat gagtttttcg cgctcgtgtt tacagttgcc
    19861 gatgatgtac acgcccagct tgtcgaagag cttggcgatt ttggggtagg ttgccgcgtc
    19921 caaaccgtgc gagtggtaga tgattttggt tttcggcgaa acgattttgg cggcaagggc
    19981 gcaggctgga acaacgcgcg ccatttggca gtcgatgatg tcgggttgtt cgcggcgcag
    20041 catccgggcg taggcaaagg tgcttttgag gtagccggca agcccgcccc ggtaaaaatc
    20101 aaccggctgc caacggatgc ccgatgcttg cgcctctccg acaaaaggcc cgtccgaaga
    20161 agcaaggatg atgtcgtgcc cgcgccgttt gagcagccgc ccgaggcgga cggtggcggt
    20221 ttccgtgccg cccaagcccg acatagaagt ggtcaggatg attttcataa tggaaaccct
    20281 tgttttttca aataatgaaa cagtttgtgc aaatctttcc agtggcgcag gatgcagcct
    20341 gccagatata ccgcccatac gccggcaaac agggggtagt ttgccggtgt gccgaagcag
    20401 gtgtaggccg ccgaggaggt caggcagagc aatgtgtgcg tataaagcgg caggcgtttg
    20461 agcggctgcc acaggcgaca ggagctttct gtcttgaaaa caaaaaacaa ccagaatgag
    20521 gcggcacagg caaccgccgc gccgcgcgcg ccgccggacg gtacggcaag ccccagcagc
    20581 agcaggtttg ccgccagcgc gcccaaggtg gcgagcgcga tcgggcgtgt ttttcggacg
    20641 acgttcaaac cgatgccgct gatttctacc agcgtgcaaa acagcggcgg cagcatacac
    20701 gatacgacga taaaccggac ggcggcgtag ttttccggca gcaggaggga ggcgaggggc
    20761 gagaaaatgc cgatcaggca gacggcggcg gcaagcaggg cggaggcaga ttctgccgtt
    20821 gccgagaggc gggcgggcgg ggtgtttgct tcgattgcgc ggaaaatata cggtgtccag
    20881 accgttgaaa agatgctttg gaacaataat gccgccccgc cgaacgaaat acccatcgaa
    20941 taaacgccga gctgttctag gccggcatat tttttcagga acaaacggtc ggcggatgcc
    21001 agcccccaat aggcgatgct gcttagtgcg atcggtatgc cgtagcgcag gccgcgatgc
    21061 aggacggcgg atgaaaacgg tgcgcgccgg acggccttca gacggcatcg gttttgaaac
    21121 agcaaaaagg cggcggcggc aaggtttgcc agcgcgtaaa cggcggtcag gacggcggtg
    21181 ttcgccggaa agtgcagcag cccgaccgtc agcggcagca gcagcaggat ggcgagcttg
    21241 gacacgagtt gcgcggacga aaaggcaagg gcgcgccctt ccatacgcaa aaccagtaag
    21301 agaaagcgga tgggcaggaa gctcagttca aacagcacca gcccgatgcc ggcggcggca
    21361 tcgtcgagcg aaaacaggat ttcagacggc agggacgggc gggaaagcag cagggcggct
    21421 atcgcggcgg cagacagcag cggcggcagg aacagggttt tgaacaaggt gtctttgtcg
    21481 gcggcggcat agtattcgcg gacgtatgcc tgatccagcc cgaggcacaa taccgacaca
    21541 gtcagtcccg ccgccgtctg catcagcacg atgcgcccga tgtcgtcggc ggggaaatac
    21601 cacgacagca gcggcaggat gatgacggct aaaaccgcgc tgccgatcga gcctgccgcg
    21661 tagccgagga tttcttttgt gtccattttt gatgtccggg cggcggcggg atgctgcctg
    21721 tgtcgtctga agcctttctt gatcggaatt tgacggcttt cagaccgtcg cggctgccgg
    21781 cggggtgcgg cagtccgggt cgcgcttttc cgggcgggcg gcggtctgaa cggggccgtt
    21841 ttttatcggc gttattatat agtgaaacgg cgcaaacctt ttaaaaggcg ttaccgtttt
    21901 ttcggaacac ggttttgatg tcgtgtccga ggatttcggt ggaaacgggc atccacaagg
    21961 gcggtgcgga aagggcggcg cggttttcgg gcagggaaaa caggtctttg ccgctgccga
    22021 ggattttggg cgaacggtac aggacgattt cgtctgccag attttctgcc aaaaatgcag
    22081 atgtgagttc ggagcctgct tcgactatga gttcccctat gccttcgtcg gcaagcaggc
    22141 gcatcaggct gttcaaatcg attttgccgc ctgccgtttc agacggcatc aggatgcgga
    22201 cgtgggcgtg tttgcgatag gggcggagtt tgtcttcatc gcgttccaag gtggcgatgt
    22261 aggttggaga ttgtccgtcg gtcagaaggt ggctgtccaa gggcaggcgc aggcggctgt
    22321 ctaaaacgat gcgtgcgggt tggcgcaaag ttggaaaagc gcggacgttg agccggggat
    22381 tgtccgccaa caccgtgccg atgccggtca gcaccgcgca gctttcggca cgcaaaacct
    22441 gtacgtcggc acgcgcgtct tcgccggtaa tccaaaagct gctgccgtct gaaagggcgg
    22501 ttttgccgtc cagcgaaacg gcgcatttga ggcggacaaa ggggcggcgg cgttcgatgc
    22561 gcgacaggaa gcctcggttg agttcccttg cctgatgttc gagtaaaccg cattccgtct
    22621 tgatgcctgc tgcttcgagc agggcaagcc ctttgcctgc aaccagcggg ttggggtcgc
    22681 gcatggcggc aacgacgcgg gacacgcccg cccgcaccag tgcttcggcg cagggcggtg
    22741 tgcgtccgta atggctgcat ggttcgaggg taacaaaggc ggtcgcgcct tgagccattt
    22801 cgcccgcctg atgcagggcg tggacttcgg cgtgcggttc gcccgctttg acgtggaagc
    22861 cctgcccgac aacttggctg ccgtgtgcga taacgcagcc gacgcgcgga ttgggcgaag
    22921 tggaaaaacg ccccaaagcg gcaagtcgga gggcggtttc catcatggat atatctgtgt
    22981 ccgaaaacat ggggataccg tatcagtatg ggttggggag aatcaggctt tgcggtctgt
    23041 tttgacggct tgcgccagcc acgaggcaaa gtctgccgga ttgtcgaagc gtttgtgcag
    23101 ggcggcgaaa cggacggcgg cttccgtatc ttgtcggagc agttctttta gcaccatgtc
    23161 ggcaagtgcg gcagagggga tgtcgcgctg acccgaagtg tagagcctgt gttccgtcag
    23221 gcggacggtt tcgtcgatct gttcgggtgt cagggcggat tttcgggcgg cggcggtcag
    23281 gtcgttgcgg aggcgttgtg cattaaaggg cgagcgtttt ttgtccggac cgatgacggc
    23341 gggcattttg agttcggcgg tttcgagcgt gccgaagcgt ttgccgcagc tggggcagtg
    23401 gcggcggcgg cgcacggcgt tgcgttcttc catcaggcgc gaatcggcga cgcgggtgtc
    23461 ggggtgggcg caaaacgggc atttcatagc gtttcttcct gatgccgtct gaacgtcaaa
    23521 ccgatacgcc ggcggcgcgg gcgatttcca gaccttcttc ggcactcata tagacggggt
    23581 tttcgggacg gtcgtgtcgg acgatgttgc cttcgcggaa catgaccagt tcgtccacgg
    23641 caagttggga ccatgattcg tcgcgggtca gcggcagggt ggcgataacg gcgacgcggt
    23701 cgttgggcgt ggttacttcg gcaaaatcga ccatcacgtc gtcgtcgagc aggcgcgcct
    23761 tgccgaacgg ggcttggcgg acgatgtagt gcagcagtgt gctggcgtgg gcaaacaggg
    23821 caatgccgtc tgaaagcata aagttaaaca gcccgaactt gcggatttcg tgcgtcagcc
    23881 ccgcaatcgc gtcaaacagc gtgtcgtcgt cgggacgggc ggcaaagcgg gtgcgcaggc
    23941 ggttgaggat gtggcagaac gcgcgttcgg aatcggttgt gccgacgggg tggaaaaatt
    24001 cgccctgttc gggaaaaaaa tcaatcaaat gtccgttgtg ggcaaacagc cagtagccgc
    24061 cccacatttc gcgcataaag ggatgggtgt tcgccagcga ggtttgtcct tgtgatgctt
    24121 tgcggatatg ggcgacgacg ttttccgatt taatctggta ggcgcgcacg aggtcggcga
    24181 cgggcgaatt ggcactgggt ttgtcgtcat ggaacaggcg cacgcctttg ccttcgaaaa
    24241 agccgatgcc gaaaccgtcg gcatggtggt cggtaatgcc gcccctgcgg cggaagcctt
    24301 caaaggaaaa cataatatcg gtcggcgtat tgcagttcat gcccagcagt tgacacatgg
    24361 tttgtcccaa tgattcagat ggtcgcaagt attcggatta taccccgaac tgaaaatgcc
    24421 gtctgaaata cggcttgttc cccgttattc cctcgaaaac agaaaacaga aaacaaagac
    24481 ggaaacttaa gattccgtca ttcccgcgca ggcgggaatc cgacttgtcc ggttttggtt
    24541 gtttttcgtt ccgtaacttt tgagccgtca ttcccgcgca ggcgggaatc tggaatttca
    24601 atgcctcaag aatttatcgg aaaaaaccaa aacccttccg ccgtcattcc cacgaaagtg
    24661 ggaatctaga aatgaaaagc aacgggaact tatcggaaac gaccgaaacc gaacggactg
    24721 gattcccgct tttgcgggaa tgacggcgac agggttgctg ttatagtgga ttaacaaaaa
    24781 ccggtacggc gttgtctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac
    24841 gagtgaatcg gttccgtacc atctgtattg tctgcggctt cgtcgccttg tcctgatttt
    24901 tgtgaatcca ctat
//
LOCUS       DAXHTY010000019        24759 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXHTY010000019 DAXHTY010000000
VERSION     DAXHTY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24759
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.019"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            1..1074
                     /locus_tag="ACFNMK_000674"
                     /pseudo
     CDS             1..1074
                     /locus_tag="ACFNMK_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675858.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(1120..3309)
                     /locus_tag="ACFNMK_000675"
     CDS             complement(1120..3309)
                     /locus_tag="ACFNMK_000675"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC6363990.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSVRTAFSDEPLLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYVLNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPILRSYHGQASHAGFVLNAGQRQASDEIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMSGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVDRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNKIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            3448..4230
                     /locus_tag="ACFNMK_000676"
     CDS             3448..4230
                     /locus_tag="ACFNMK_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC6363991.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(4643..6577)
                     /locus_tag="ACFNMK_000677"
     CDS             complement(4643..6577)
                     /locus_tag="ACFNMK_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC6363992.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(6643..7821)
                     /locus_tag="ACFNMK_000678"
     CDS             complement(6643..7821)
                     /locus_tag="ACFNMK_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC6363993.1"
                     /translation="MAKMMKWAAVAAVVAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8047..8436
                     /locus_tag="ACFNMK_000679"
     CDS             8047..8436
                     /locus_tag="ACFNMK_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6363994.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFKTYDKNKLKQNEYFDITLSEVSPDHFTLKATPNPTTND
                     GETCFVTLNDGGTIAASGTNQSCPGFD"
     gene            8719..9765
                     /gene="adhP"
                     /locus_tag="ACFNMK_000680"
     CDS             8719..9765
                     /gene="adhP"
                     /locus_tag="ACFNMK_000680"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC6363995.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(9930..13415)
                     /gene="smc"
                     /locus_tag="ACFNMK_000681"
     CDS             complement(9930..13415)
                     /gene="smc"
                     /locus_tag="ACFNMK_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC6363996.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENESQVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQNR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQASDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPHGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLITQKAHLDGIASELDNLAPELSADEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIARELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIAQLEQQTLDWQERQQELALAYETEFQNDEQHIKLDELT
                     EAVQTLDEEYIAVQEKLAQIQAQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            13417..13668
                     /locus_tag="ACFNMK_000682"
     CDS             13417..13668
                     /locus_tag="ACFNMK_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6363997.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQRPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            13665..14387
                     /gene="lpxH"
                     /locus_tag="ACFNMK_000683"
     CDS             13665..14387
                     /gene="lpxH"
                     /locus_tag="ACFNMK_000683"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC6363998.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAIYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDKAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            14656..16242
                     /locus_tag="ACFNMK_000684"
     CDS             14656..16242
                     /locus_tag="ACFNMK_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC6363999.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPIAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(16410..16613)
                     /locus_tag="ACFNMK_000685"
     CDS             complement(16410..16613)
                     /locus_tag="ACFNMK_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC6364000.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            16729..17226
                     /locus_tag="ACFNMK_000686"
     CDS             16729..17226
                     /locus_tag="ACFNMK_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC6364001.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            17308..18501
                     /locus_tag="ACFNMK_000687"
     CDS             17308..18501
                     /locus_tag="ACFNMK_000687"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC6364002.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(18905..19840)
                     /gene="hemC"
                     /locus_tag="ACFNMK_000688"
     CDS             complement(18905..19840)
                     /gene="hemC"
                     /locus_tag="ACFNMK_000688"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC6364003.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            19959..20474
                     /gene="ybeY"
                     /locus_tag="ACFNMK_000689"
     CDS             19959..20474
                     /gene="ybeY"
                     /locus_tag="ACFNMK_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC6364004.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARDYNRDYRGKDYATNVLSFALNEGETLPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPEQHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GFPNPYQEDGH"
     gene            20476..21300
                     /locus_tag="ACFNMK_000690"
     CDS             20476..21300
                     /locus_tag="ACFNMK_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC6364005.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVIIGSLQFTVARADNRRLHTLMATHVK"
     gene            complement(21443..22822)
                     /gene="nhaC"
                     /locus_tag="ACFNMK_000691"
     CDS             complement(21443..22822)
                     /gene="nhaC"
                     /locus_tag="ACFNMK_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC6364006.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23113..24336)
                     /locus_tag="ACFNMK_000692"
     CDS             complement(23113..24336)
                     /locus_tag="ACFNMK_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC6364007.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         5634 a         7113 c         6410 g         5602 t
ORIGIN      
        1 tgcggccgtt tttcgtcggt gcggcggtac ttgccatact cggcgcgttg gtgtttttca
       61 tcagccccgg tgccgtcgtc ctgcaccgcc aaatcttctt agaactcatg ctacctgcgg
      121 catatggtgg tttcctgact gcggctttgt tggactggac ggtttttttc gggtaacctg
      181 aaacctgtcg cgactttgat ggcggcatta ttgctcgccg catccgctat actgcccttt
      241 tcgccgcaaa ctgcctcgtt tttcgtcgcc tcctattggc tggtgttgct gctgttctgc
      301 gcccggttga tttggctggc ccgcaacacg gataacttcg ccctgctgat gctgcttgcc
      361 gcgttcaccg tttttcagac ggcatacgcg gcaaccggcg acttgaacct gttgcgcgcg
      421 caagtgcatt taaatatggc tgcggtgatg tttgtttcca tacgtgtgag tgttttgctg
      481 ggcgcggaag cccttaaaga atgccgtctg aaagaccccg tattcatccc caacatcgtc
      541 tataaaaaca tcgccatcac cttcctgctg ctgcacaccg ccgccgaact ttggctgcct
      601 gcgcaaaccg ccggttttac cgcgcttgcc gtcggtttca tcctgctcgc caagctgcgc
      661 gaactgcacc atcacgaact cctgcgcaaa cactacgtcc gcacttatta cctgctccag
      721 ctttttgccg ccgcaggcta tttgtggaca ggtgcggcga aactgcaaaa cctgcccgcc
      781 tccgcgcccc tgcacctgat taccctcggc gacatgatgg gcggcgtgat gatggtgtgg
      841 ctgaccgccg gactgtggca cagcggcttt accaagctcg actacccgaa actctgccgc
      901 atcgccgtcc tcctgctctt tatggccgcc gtctcgcgca ctttcttgat gaacgtgaac
      961 ccgatattct tcatcaccgt tcctgcgatt ctgaccgccg ccgtgttcgt gctttacctg
     1021 ctgacgttcg taccgatttt tcgggcgaat gcgtttacgg gcgaccctga ataaaaatgc
     1081 cgtctgaaac ggtttcagac ggcatcggat tgccgataat caagcctcct gcggatcgac
     1141 atccaccgac catctaattt tgccgtcgcg gttttgctgc aacacctgca cccacaaact
     1201 cacggcacgg tgcaaatcct gtcgggacgg agattcgagg aaaatttgcg cgcgttcgcg
     1261 ttcggcgagg cgcaccatca gcatgggggc ggcgccaaac tgggaaacgc ttgcgggcaa
     1321 cagcggggcg agggtttctt tggcggcatt gagaaattcc agcgcatcgg caacacgcgg
     1381 cgcgtccgcg cggacggcgg tctgaaaacc gaagggcggc atggcgaaca tttgccgttc
     1441 gttcaattcg ttttcggcaa acacggcata atcctgcgct ttgacggcgg cgaagacggg
     1501 atgttcgggc agttgggtct gtatcaacac cttgccgggt ttgtcggcgc gccccgccct
     1561 gccggacacc tgcatcagct cggcgaacag cctttccggg gcgcgaaagt ccgcgctgta
     1621 caggctgccg tcagcgttca acacgataac gaggttgagc cgcgcgaaat catgcccttt
     1681 ggcgagcatc tgcgtgccga ccaaaatgtc gattttgttg tcggcgatgc ggcggtacaa
     1741 atccgcccag tcgtttttgt gcgccgtgct gtccctgtcg acgcggacaa cggctgcctt
     1801 gggcaggaag gcgcgcaggg tttcttcgac gcgctgcgtg ccgtgtccga cggcggtcag
     1861 gtcttggttg ccgcagtcgg ggcatttgaa cgggatgggt tcgcggtggt cgcagtggtg
     1921 gcagcgcagt tggcgggcgc gttggtgcaa caccattttg gcggagcagt tcgggcagcc
     1981 gaaggtatgg ccgcagtcgc cgcaaaacag cgcgggcgcg aagccgcgac ggttgaggta
     2041 caccagcgac ataccgcccg cttcaaagtt ctgcttcaaa agttgcaagg cttgcggcga
     2101 gaagccgttg tcaagtttca gacggtctac gttgaggatg tccacttgcg gcagttgcgc
     2161 ggcggtatgg gcgcgttcgg tcagttgcaa gaggcggtac gcgccgcttt gcgccttgtg
     2221 ccagctctcc aagcttgggg tcgcgctgcc caacacgacg ggacagccgc cctgcttcgc
     2281 acgccacacc gccaaatcgc gggcgtggta gcgcaattcg ttgtcctgtt tgaacgagcc
     2341 gtcgtgttcc tcatcgacca caatcagccc gacatcatcc atcggcgtga acaccgccag
     2401 ccgcgtgccg atcaccagct tcgcctgccc cgacatcgcg cgcagataat cctgcgttcg
     2461 cctgcctgcc gccatccggc tgtgcaacac ggcggtcggc acgtcggcaa aacggttttc
     2521 cacccgcttc aaaagctgcg gcgtaagatt aatttcgggc aacagaaaca acacctgccg
     2581 cccctgcgcc aacactttcg ccatcgcatc aaaatacacc tcggtcttgc cgctgccggt
     2641 gatgccgtac agcaaaaacg gctgaaatcg tcccaatgcc gtctgaatct catcggaagc
     2701 ctgccgctgg ccggcgttca acacaaatcc ggcgtgcgaa gcctgcccgt ggtacgacct
     2761 taaaatgggc ttcgccgctt ccgtcgtttc gatccagccc tgctccgccc aatcttcgat
     2821 taatttcgcc gcctgcgcgt tcacctgctt caacgccgcc atcgtcatcc cgcccgacag
     2881 cagcgcgtcc cacagagccg cttttttgtt gaaccgcgcc ggcggcggcg tttgtgccct
     2941 gcccgcttcg ttcagcacgt aaaacaacgg cggctgcggc atttccaccg cgcgcgtttc
     3001 cttcaaacct tgcggcaatg cggcaaatac tgcctgcccg gtcggatagt ggtaataacg
     3061 cgacgtaaac gccaacaaat cacgccagct ttcgggcagc agcggctcgt ccgaaaaggc
     3121 cgtccgaacg ctcaaaatcc gcgccgtatc catatccggc gcaatatccg tttcccacac
     3181 catcccgacc acggttttat tgcggaaagg cacaagcacc cgcgttcccg gaggaagcgg
     3241 atcggaatgg gaataagtca aaaggccgtc tgaaagcggc acgtttacgg cgatgcggtg
     3301 gtagatcata caggcaggca aatcattttt ttgcgtattt taacaggcgg aagctatttt
     3361 cagacgataa attgagccta ggcgcaatgc aggcgcaaca gagatttgat acaattccgc
     3421 ttttccaaca cgcgcaatca tctttatatg aaaaccaagt taatcaaaat cttgaccccc
     3481 tttaccgtcc tcccactgct ggcttgcggg caaacgcccg tttccaatgc caacgccgaa
     3541 cccgccgtca aagccgagtc cgccggcaaa tccgttgccg cctctttgaa agcgcgtttg
     3601 gaaaaaacct attccgccca agatttgaaa gtgttgagcg tcagcgaaac accggtcaaa
     3661 ggcatttacg aagtcgtcgt cagcggcagg cagattatct acaccgatgc cgaaggcggc
     3721 tatatgttcg tcggcgaact catcaacatc gacacgcgca aaaacctgac cgaagaacgc
     3781 gccgccgatt tgaacaaaat cgacttcgcc tccctgcctt tggacaaagc catcaaagaa
     3841 gtgcgcggca acggcaagct gaaagtcgcc gtcttttccg accccgattg tccgttctgc
     3901 aaacgcttgg aacacgagtt tgaaaaaatg accgacgtga cggtttacag ctttatgatg
     3961 cccattgccg gcctgcaccc tgatgccgcg cgcaaggcgc aaatcttatg gtgtcagccc
     4021 gaccgcgcca aagcgtggac ggattggatg cgtaaaggca aattcccggt cggcggcagc
     4081 atctgcgaca atcccgtcgc ggaaaccact tccttgggcg aacaattcgg cttcaacggc
     4141 acgccgaccc tcgtcttccc caacgggcgc agccaaagcg gctacagccc gatgccccaa
     4201 ctggaggaaa tcatccgcaa aaatcaataa tccatccgca aaaaaggctt ggaaaattcc
     4261 aagccttttt tgcgggaaat gccgcctgaa aaccaatccg ccctgaaagt tagcaagcca
     4321 acaaacacat cctaacccat tgaatgattg aaaataataa attgaacaat acgaagcccc
     4381 gccgccgttc ccacgcgggc gggaatctgg aatattgagt taccgtgatt tatccgaaac
     4441 aagcaaagcc gaacgaaccg aattcccgtc tttacgggaa tgacgggact cgagcgttgt
     4501 gtgcaatcat cgaacccccg ccgccgtcat tcccacgcaa gtgggaatct agaacgtaga
     4561 atctaagaaa ccgttttgtc cgataagttt ctgctccaaa atgaccggtc cggcagcttc
     4621 aaacggcatc tttgtgccaa cctcaatcct gcgccaatgc gtctatcgga ttgagtttgg
     4681 ctgctttatt ggcaggcata aagccgaacg cgatgccgat tccggtcgag caggcgaccg
     4741 ccccgataac ggatgccgcc gaaatgtcca tcgggaagtc ggttacaaaa tgattgaaca
     4801 cgaggctgac ggcggcggac aaacccacgc cgaccaaacc gccgatgacg cagattaaca
     4861 ccgcctcaat caaaaactgc tgcaaaatat tgccgcgccg cgcgccgatt gccatccgta
     4921 tgccgatttc tttggtgcgc tcggtaacgg acaccagcat gatgttcatc acgccgatgc
     4981 cgccgactac caatgaaatc agggcgatgg aggaaatcag cagcttcatc gtaccggtgg
     5041 tgctttcgac tatctgcctg atgctgtcgc tgttgttcat gaagaaatct tccgtgccgt
     5101 gccgcgcttt gagcagatcg gtcagccctt tttcggcaac ctgggtattg gcattgtctt
     5161 tgattttgac ggtgatggag ttggtgtggc tctcgcctgt gatttggtgc atcaccgtcg
     5221 tatagggcga ccaaagcatc agcacgtcgg aattgccgaa agcgttttcg tcttttttca
     5281 tcacgccgat gacggtcaag gggcgtttcc tgaacaaaat ggttttaccc aacggatccg
     5341 agtccgcaaa gagtttgtct ttgacatttt ggtcgatgac gacgacctgc gcgtcttctt
     5401 tcacatcgtt ttcgtcaaac agccgccccg tttccagctt cagtccgcgc acgtcgaaat
     5461 attgttcgcc cacgccgtaa agcgaggcgg tcaggtcggt gttgcggtaa gtcagcgtgc
     5521 cgccgctcga agtcatgggc gtggcggaag caacgtagct ttgtttggcg atgatttttg
     5581 cgtcgtctat ggtcagggtt ttaatcctgc cgctgcgcct gtcgccgaag ccgcgccccg
     5641 ggaagatgct gatggtgttc gtccctatcg aactgatgtc ttcaaggatt tttttctgcg
     5701 aaccgttgcc caatgcgacg acggagacaa ccgaagcgat accgatgatg atgccgagca
     5761 tcgtcagaag cgaacgcatt ttgtgcgcca atactgcttg caccgacatt ctgaaggctt
     5821 cgacaaactg gtcgtaataa aacgaccacg aggctttttc ccgaatcctc ccgacattgc
     5881 ttgcggggat ttcgggattt ttcgaggtgt cggaaatgat ttcgccgtcc cgaatttcga
     5941 tgacgcggtt ggcattggcg gcgatgccgg ggtcgtgcgt gaccataatg acggtatgcc
     6001 cggcttcgtg cagcttatgg atgatttcca tcacgttttt gccgctggcg gtatcgagcg
     6061 cgccggtcgg ctcgtcggcg aagatgattt ctccgccgtt catcagggcg cgggcgatgg
     6121 agacgcgctg ctgctgtccg cccgagagtt cgccgggctt gttgccctct ttgcttgcca
     6181 aacccaaatc ctgcaagagt ttgtccgccc gcgcggaacg ctctttgccg cccgcgccca
     6241 tatagacggc tggcagcgcg acgttatccc ttgcggtcag cgagcttaag aggttgtagc
     6301 gttggaagat gaaaccgaag cgttcgcggc gcaatgccgc cagctcgtca ggctgcattt
     6361 tggcagtttc gatgccgtcg attcggtacg aaccggaacc ggcggtatcc aaacagccga
     6421 gtatgttcat cagcgtggac ttgcccgaac cggactgccc gatgatggcg acaaaatcgc
     6481 ccttctctat cgacaggctg atgtctttca aaatatggac gcggttctcg ccgctgccga
     6541 aatagcggtt gatgttttta cattcgatca agctcataat attccgtaaa tcaataacaa
     6601 atgccgtctg aaaccgtttc cgtgttcaga cggcatattc gtttatcggc gcggcgggcc
     6661 gcctagggcg cgttcgccgc tttcctgttg ctcggcggcg gttatttcgg agatgaccac
     6721 tttgtccccc tctttcaacc cgctttttac ttcggtattc atactgtctc tcataccggt
     6781 ccggatttcg cgttccgccg ccttgccgtc cgcacccaac acgcgcacaa acgccttgcc
     6841 gccgcgattt ttcacggtca gcgacggaat aatcagcaca tttttcacgc cgtcgatttc
     6901 aaccgtattc tgcgtcgtca tccccgtggc gagtttgccg tccggattcg gcacaaacga
     6961 acgggcataa tagtagaccg cattggaagc cgtatccgta ctgctgttgt agccgcccga
     7021 cgacatcgtg gtcagcccgg ggtcgacgct gtcgagcttc gccttaatcg gcgtatccgg
     7081 ttcggacaaa atcgtaaacg aaatatcctg ccccgccttc accttggtaa tatcgccctc
     7141 ggcaatctgc attttgttca acatcatatc cagattcgcc aattggacaa tcgtcggcgt
     7201 agactgcgcc gcgttcacag tctgcccctc ttccacgaga atcgccacca ccgtgccgtc
     7261 catcgttgcg gtaatgcgcg tgtagcccaa ttccgactcg gcggtattga tggaaatttt
     7321 gctctgtctg attaaagcct tcagctcggc aacattggct ttggcggcgg caaacgcatc
     7381 ctgcgcgctt tccaaatcct ctttggaagt cgcgttttcc ttccataacg ccgcctgacg
     7441 cttatatttc ttctccgcac tgcccaatgc aatctgtgcc gacaccagct tcgcctgata
     7501 cgtttccaac ttggattttt ccgtattgag cgtattggtc tgcgaggtcg aattgatttc
     7561 cgcaatcaaa tcgccctttt taacctgttg cccgagtttg acataaagtt tcttaatctg
     7621 ccccgatgcc tgtgcgccga ccgataccag gttggacggc gaaatctccc ctgttgcaga
     7681 aaccgtccgg ctgatgtcgc cgcgcctgac cgtttccgta atataagcag cctgcggctc
     7741 gggcttcaga taagaccatc cgccccaaac cgctgccgcc acgaccgccg caacagccgc
     7801 ccatttcatc atttttgcca tgttttcaat tctctgtatg cgtcaatccc ataaatcgga
     7861 agcggattct accgcaagtc cctgccccat ccaaggcggc aaactgccga caataaaaat
     7921 aaccatttgt tttaaatggc ttatacaatc tgctttcccg aaaatttcaa tccgtttaca
     7981 tattccgcca tttccgatac ggcgttataa tgccgtcgac cgacaaacaa atggaaacac
     8041 attgctatga aaaacgttca aaaaggcttt acgctgctcg agctgatgat tgccgtcgcc
     8101 atcctcggca tcctgacgct catcacctac ccttcctata aaacctacat ccggcgcgtc
     8161 cgcctgtcgg aagtcaggac gaccctgctg cacaacgcgc agaccatgga acgttactac
     8221 cgccaaaaag ggacgtttaa aacctacgac aaaaacaaac tgaaacaaaa cgaatatttc
     8281 gatatcactt taagcgaagt cagccccgac cattttaccc ttaaggcaac gcccaatccc
     8341 accaccaacg acggcgaaac ctgcttcgtt acgctcaacg acggcggtac cattgccgct
     8401 tccggtacaa accaatcctg ccccggcttc gactaatcct ccgccgaagc ccgcaaaccc
     8461 gtaaagatgc cgtccggaca cagcttcaga cggtatcttt acgggtttgc cgtccggttt
     8521 ttcaaacttg aaacctgccg cccctcatcc ggcaaccgcc ccgatgccgc gcctttccaa
     8581 gctgttatag tataacatat aacccatatt tttcaacaac ataccacttg acctgtgaga
     8641 tgcggaagcg tatgcttacc cttatatcgg acaacattcc gtttacaggt ttttttgccg
     8701 taactaagga gcgcaatcat gaaaatgcag gcagttgttg tgaataaaaa tgtagcgggc
     8761 gatgtggaaa tagtcgaacg cgaggttcgc ccgttggaat acggcgaggc gttggtcgaa
     8821 gtcgaatatt gcggcgtgtg ccacaccgac ctgcacgttg cggcaggcga ctacggcgaa
     8881 aaaccgggcc gcgtgctggg acacgagggc atcggtttgg ttaaagaagt tgccgacggt
     8941 gtgaaaaatc tgaaagtcgg cgaccgcgtc agcatcgcct ggctgttcca aagctgcggc
     9001 tcgtgcgaat actgcaatac cggccgcgaa accctgtgcc gctctgtatt gaacgcgggc
     9061 tacaccgccg acggcggtat ggcgacccac tgcatcgtga gtgccgatta cgcggtcaaa
     9121 gtccctgaag gtttggatcc tgcgcaagct tcgagcatta cttgtgccgg cgtgaccact
     9181 tataaagcca ttaaagtttc cggcgttcgt ccgggacagt ggattgccat ctacggcgcg
     9241 ggcggtttgg gcaacttggg ggtccaatac gcgaaaaaag tattcggcgc gcacgttgtc
     9301 gccatcgaca tcaacgacga caaactggcg tttgccaaag aaaccggcgc ggatttggtt
     9361 gtcaacgccg ccaaagaaga cgctgccaaa gtgattcagg aaaaaaccgg cggcgcacac
     9421 gctgcggtcg taaccgccgt atctgctgcc gcattcaact ctgccgtgaa ttgcgtccgc
     9481 gcaggcggac gtgtggttgc catcgggcta ccgccggaat cgatggattt gtccatcccg
     9541 cgtttggttt tggacggcat cgaagtggtc ggctctttgg tcggcacgcg caaagatttg
     9601 gaagaagcct tccaattcgg cgcggaaggt ttggttgtgc cgaaagtcca actgcgtgct
     9661 ttggatgaag cacccgccat tttccaagaa atgcgcgaag gcaaaatcac cggtcgtatg
     9721 gtaatcgata tgaaaaaaga atgcggctgc ggccaccacc actgatttga cgtggcgata
     9781 cacatcgaaa tgccgtctga acgctgttca gacggcattt tttatggatt tggatttgat
     9841 tttaatctgt tctgtttgaa ataccgtctg aaaacccgta tcgcaacacg tcattaagaa
     9901 cggcaagatt cagccgttct gcaacccgtt caaacggctt ccgccatttc caacgcctgc
     9961 ttgatgtcca cggcgacgac gcgcgatacg cctttttcct gcatggttac gccgaccagc
    10021 tgctctgcca tttccatggt caggcggttg tgggagatgt agagaaactg ggtttgcgcc
    10081 gacatttctt tgaccaggtt gcagaaacgc gaagtgttgg catcgtctag cggagcgtcg
    10141 acttcgtcca aaagacagaa cggagcgggg ttgaggctga acagggcgaa cacgaggctc
    10201 atcgcggtaa gggctttttc gccgccggaa aggaggtgga tggtgctatt tttcttgccg
    10261 ggcgggcgcg ccatgataga cacgccggcg gtcagtaggt cgtcgcctat cattttgagg
    10321 gtggcttcgc cgccgccgaa cagggtcggg aagaaggttt ggactttgcc gttgacggcg
    10381 tcgaaggttt ctttgaaacg cgctttggtt ttgtcgtcga tttgggcgat ggcttcttcc
    10441 aaaatggtga tggctgcctg cacgtcttcg ctttggctgc ggtagtagcc gtcgcgttcg
    10501 cgcgcttctt cgagttcttg cagggcggcg aggttgaccg cgccgagggc ttcgatttgt
    10561 tgcgagaggc tgccgatgct gctgttcaat actttcggcg attctttcgc caacgcttcg
    10621 agcgcatcca aatcggcggc gcgttcggtc aggttttgat ggtagcgttt ggcgttgatc
    10681 agggcttcct gttgttgcaa cagggcggtt tgggtggctg cctgaagctg tggcaactgg
    10741 gtttgcaggg tttgtacgcg ggcgtattgt tccctgccct gcgcctgaat ctgcgcgagt
    10801 ttttcctgca cggcgatgta ttcttcatcc aaagtttgta cggcttcggt caactcgtcg
    10861 agttttatgt gctgctcgtc gttttggaac tcggtttcat aggctagggc aagctcttgc
    10921 tggcgttcct gccagtcgag ggtttgctgt tcgagctggg caatttgctg ttggtagttt
    10981 tgtttttgct ggttgagctt gtgaacggcg acttcggcaa gcccgtattg gcggttggct
    11041 tccaacaggg caagctgcgc ctgtttcaga cgaccttgct gctcttggcg gctgtgcgcg
    11101 gtggtttgct gttggtgttc gagttcggcg gcggcttcct gcaaagtggc gatgtcgtct
    11161 gaaagcccgt cggacgtgtg ttgcaacacg gtttgctctt ctgccaactg cgccagttca
    11221 cgggcgatgt gttcgcggcg gatttgccct tggttggtgc gcgccagaag ttcggcggcg
    11281 cgttgctgcg cctgactgta ttggcgcgtg tgctgctgtt gttgctgcat caggtttttg
    11341 tgctgcactt cggacgagcg cacggcggct tccgcctgtt tgaacgcggc ttcgtcggcg
    11401 gagagttcgg gggctaggtt gtccagttcg gatgcgatgc cgtcgaggtg cgctttttgg
    11461 gtaatcaggc tttcctgcgc gggcttggca tagagcagga cgctgacttt atcgacctga
    11521 tgaccttcgg gcgtgagcca gatttgatgt gcacccaaat cgttttgatg cgcgagggcg
    11581 tagctcaaat cgggcgcgca caatacgccg tcgagccagt ggtgcaatgc cgtctgaaac
    11641 ggcggctgcg cttggatttg gttcagcaat gcctgtacgg gcagggattt tttgatgccg
    11701 ccggagaggc cgtctgaaag ccatgccgcc tgcccctgcg gcaaaggttc gggcggtacg
    11761 aagccgtgcg gcacggcgcg ggcgtgcagg cgttcggcaa ggatgacgga caaggcgtgc
    11821 tgccactcgg cgggcgcggt gatgtgttgc cacagttgcg gcgcggcggc gtggtcgctt
    11881 gcctgccaga agtcggcggc ttcctgctgt tgcgacagga tttgcgacaa cgcctgctgc
    11941 tgcgcctgca aggtgatgtg ttgctgcttc aggctttgga agcggtttga ggccgtctga
    12001 aacgcctcgc gggcggcgtg taaggcttct tcggcggcga tgatttgttc ttcgtaatgc
    12061 tcttgccggt tttgcaacag ggcggcggct tcctgcgcgg cggcggtttc ggcttcgtcg
    12121 ggcaggttca gggcttggtt ttcctgtttc agacggcctt tgcgctcttc gtgcttggca
    12181 acggtttgtt cggcatgggc aagctgctgc tgcttcaacg ccagttctcg gcggatgcgg
    12241 tttgcctcgt cctgctgggt ttggaaggcg gcattgagcg tggcttgggc ctcttccaat
    12301 tcgggcagac gctcttcgtg ttcggcaacc tgcatcgccc attccgccaa ttcggtttgc
    12361 ttttcttcga cctgcgactc gttttcttcc agctgcacgc ggatttgctg ctgctcctga
    12421 tggatgcgct gcaactgcgc ctgcgccgcc tgcttgtcgc gttcaatgcg ttggtgcagg
    12481 ttttgccggt ggcggatttg ctcttccaaa cgggctatct gctcgcgcaa cacgccgcgt
    12541 ttgttgccca attcgtgcac cgcctgctgc tgcgactgtt cggcggtctg caaggcatgt
    12601 acttcgtcgt ttaacgcctg aacctgcgcg gcggtttcgt cctgctgcgc ctgcaaagac
    12661 tgatgctgcg cggtcgcttt gtcggcggcg gcaagcgatt gctgccattg ggcgtaatcg
    12721 agcaaatcct gttgttggtt caactgcgcg gtcagggatt tgtagcgttc ggcggtttcc
    12781 gcctgtttct ccagcttttc cacctgacgc gccaactcgt tctgcaaatc gcccaaacgc
    12841 tgcaaatgct cgcgcgtgtc tttcagacga ccttccgtct ccttgcggcg ttccttatat
    12901 ttggacacac ctgccgcctc ctcgatatag gcgcgcaatt cctccggccg cgcttcgatg
    12961 atgcgcgaaa tcatcccctg ctcgataacg gcataaccgc gcgcgcccac gcccgtaccc
    13021 aaaaataaat cggtaatgtc gcggcggcgc acggtctgat tgttgatgaa ataagtcgat
    13081 tcgccctgac gcgtcagctg ccgcttgatg ctcacctcgg catactgccc ccacgccccc
    13141 tgcaaactgt ggtcgctgtt gtcaaatacc agctccaccg aagccctcgg cgcaggacgg
    13201 cgcgtcgccg caccgttaaa aatcacgtcc tgcatactct cgccgcgaag ctgcttcgcc
    13261 gaagcctcac ccaacaccca gcgcaccgcg tcaatcacat tcgacttgcc gcagccgttc
    13321 ggcccgataa ccgcgacaag ctgccccggc acatgaatcg tggtcgggtc ggtaaaagat
    13381 ttgaagccgg agagtttgat atgggtcagg cgcataatgg tcggaaaaaa taaaaaagaa
    13441 gcgtatttta acggaaatcc cgccgcacca ccccatatct tgccggcaaa accttaccgc
    13501 atcccgcacc ctcgatgccg tctgaagcct tcagacggca tttttaacgc gcccgaaccc
    13561 cgattccgcc aacgcccgat gcttggcgcg gcatttgccg tattgccccg ccgccgggcg
    13621 cgttataatc cgccacgaca tccgcccgat ttggaaacct gcctatgaaa cccgcctatt
    13681 tcatctccga cctgcatttg agcgaaaagc accccgaact gaccgcgctg ctgctgcgtt
    13741 ttttacgttc ttccgccgcc gggcaggcgc gggcgattta tattttgggc gatttgtttg
    13801 atttttgggt gggcgatgac gaagtttccg agttgaatac ttcggttgcg cgggaaatca
    13861 ggaaattgtc cgacaaaggc gttgccgtgt tcttcgtcag gggcaaccgc gacttcctga
    13921 tcggtcagga tttttgccgg caggcgggta tgacgctgct gccggattac tcggttttgg
    13981 acttgttcgg ctgcaaaacc ctcatctgcc acggagacac attatgcacc gacgacaagg
    14041 cataccaacg tttccgcaaa atcgtgcatc ggaagcggct gcaaaaactg ttcctgatgc
    14101 tgcccctaaa gtggcgcacg cgccttgccg ccaaaatcag gcgtgtcagc aaaatggaaa
    14161 aacaggtcaa gcccgccgat attatggatg tcaatgccgc ctttaccgcg cggcaggttc
    14221 gcgcctttaa cgcggaaagg ctgatacacg gacacaccca ccgcgagcat atccatcacg
    14281 aaaacggctt tacccgcatc gttttgggcg actggcataa cgactatgct tcaatcctcc
    14341 gcgtagacgg ggacggcgcg gtattcgtgc cgctggaaaa atactgaaaa tgccgtctga
    14401 agcctttcgg gcggcttttt ttacacgccc ctttcccaca ctccctccgc cgcatcatca
    14461 ccatccttaa cccgcattaa tagccattat cgataaaatg cttgaccgat tacataagat
    14521 tacgtaaagt gtgcaaacgc ataacaattg gtcttaccaa tttatctttt aaccaaaata
    14581 tcgggtgtac gggatttgaa cgtctttcca aacctcgacg cgccttatga tgcaaatgcc
    14641 taaaaaggat tcgttatggc acttttcctc agcatattcc ccatcgtcct gctgatttgg
    14701 ctgatggtga aaaaaaacag tatgccctcc tacgtcgcgc tgccgattac cgcagtgctg
    14761 atttacgcca tcaaactttt ctacttcgac gatgcgggca tgctgctcaa cgccaccgcc
    14821 gcttccggcc tcgtcaaaac gctcacgccg attaccgtga ttttcggcgc gattatgttc
    14881 aaccgtatga tggaaaccac gggctgcatc gatgtcatcc gcaaatggct ggcgaccatc
    14941 agccccaacc ccatagcgca actgatgatt atcggctggg cttttgcctt tatgattgaa
    15001 ggcgcatccg gcttcggcac gcctgccgcg attgccgcgc cgattctgat gagcttgggc
    15061 ttcaacccgt tgaaagtggc gattttcact ttggtgatga actccgtccc cgtctctttc
    15121 ggcgcggtcg gtacgccgac ctggttcggt ttcgcaccgc tgaacctgag tgccgaagac
    15181 atcctcgcca tcggcaggca gaccggcgta atgcacttct tcgcaggttt cgtcatcccc
    15241 gtcatcggct tgggcttcat cgtaccttgg tctgaaatcc gcaaaaactt gggcttcgtc
    15301 gccattgccg tcttctcctg caccattcct tatgtcgcat tggcgatggt caacgaagaa
    15361 ttcccgtcgc tcgtcgccgg cgcaatcggc ctgatggtgt ccgtattcgc cgccaaccaa
    15421 ggctggggct tgagcaaaga ccacgccaaa gacccgaatg ccgaaaaagt gccgttcgcc
    15481 caagtcgcca aagcactcgc ccctttgggt atgctgatcg gcatgctggt ggttacgcgc
    15541 atcaaacagc tcggcatcaa aggcattttg accagcaaag aagaatggtt cagcttccaa
    15601 ctgccgtttg atttgtccaa aatcaccgtc agcgactccc tgacgattac cttcggcaat
    15661 attttcggac aagatgtcag cgcgtcttac caaacgctgt acgtcccggc ttggattccg
    15721 tttgtgctga ccgtttggat ttgcatcctg ctgtataaaa ccaaattcaa agatgcctgg
    15781 acgatttatt ccgtaacttt caatcaaacc aaaaaaccgc tgcttgccct gatgggcgcg
    15841 ctgattatgg ttcagctgat gctggtcggc ggcgacaatt cgatggtgaa aatcatcggt
    15901 aaggaatttg ccgcaatggc gggcgaacac tgggtttatt tctcgccgta tctgggcgcg
    15961 atcggtgcgt tcttctccgg ctccaacacc gtgtccaacc tgaccttcgg accgattcag
    16021 cagcaaatcg ccttggatac cggcctgtcc gttaccctga ttctggcgtt gcagtccgtc
    16081 ggcggcgcga tgggcaatat ggtgtgcctc aacaacatca tcgccgtatg taccgtattg
    16141 gatgtgaaaa attccgaagg tgcgattatc aagaaaaccg ttatcccgat ggcgatttac
    16201 ggcgtgattg ccgtcgtcgc ggcaatgatt ttcttcctct aggcgaaacg ccaccatcca
    16261 tagcggcagc acgcggggaa cggaaaccgc cgttccccgt ccgggcggcg catcgggcag
    16321 aatctcggtg ccggaacagc ccgccccgcc aaacaaatgc cgtctgaaac cggaaaaggc
    16381 ttcagacggc atttttccgc cgcttgcgtt caggcgtaat gatgatgcgg catcttgccg
    16441 tcaaggcggt agcgcgtgcc gcagtacggg caggcaacgc tgcccgattc tccttcgcac
    16501 aaaggcaaaa acactctcgg atgcccgttc cactgctcgt tgtcgggtcc cgagcaatac
    16561 agcggcagat tttccggcaa cacggaaatt tcctgcagat tcagattgtc catttgattt
    16621 tcctttgcgt ggttggcgtg cgcccatttt acgccatcgg caggctaaag gatattttcg
    16681 gcgcaaagcc gcaatccgct ataatcccca cttttcagac gacataccat gactgcgctt
    16741 acccttccgg aagacatccg ccaacaagag ccatccgccc tgctctatac cctcgtttcc
    16801 gcctacctcg aacacaccgc ccaaaccggc gacgaatccc tctcctgcct gtccgacgac
    16861 cagcacacgc tgaccgcatt ctgctacctc gacagccaag tcgaagaagg cggcttcgtg
    16921 caactcatcg cctccggtta cggcgaatat attttccgca acccgcttgc cgacagcctg
    16981 cgccgctgga aaatcaaagc cgtgccgaaa gtcttggaca aagccaaagc cctctacgaa
    17041 caacacggca aaaccatcga aacgctcgcc gacggaggcg cagacatcga atccctgcgc
    17101 aaacagttcc ccgactttga agaatgggac ggcgcatact acgaagccgc cgaacaagac
    17161 ctgcccctgc ttgcagaaca catacagtca aactgggaaa ccttcgccca tatcgggcag
    17221 gcgtgattgc gtctgtttcc agcccgtgta aaacagcgta aaatcggcaa acccgaatga
    17281 ccttccgtcc ccatcaagga gcaagctatg ttcttcaagc acatcgaagc cgcccccgcc
    17341 gatccgattc tcggtttggg cgaagcgttc aaagccgaaa cccgccccga aaaagtcaac
    17401 ctcggcatcg gcgtttataa agacgcatcc ggcgcgacac ccctcgtcaa agccgtcaaa
    17461 gaagccgaaa aacgcctgtt ggaaagcgaa accaccaaaa actacctcac catcgacggc
    17521 gttgccgact acaacgcgca aacccaaatc ctgctgttcg gcaaagacca cgaaatcatc
    17581 gccagccgtc gcgccaaaac agcgcaaagc ctcggcggta cgggcgcatt gcgtattgcg
    17641 gccgagtttg ccaaacgcca gttgaacgcg caaaccatct ggatttccaa tccgacttgg
    17701 cccaaccaca acgccatcgc caaagcggtc ggtatccaag acaaacctta tcgttactat
    17761 gatgccgcca aacacggttt ggattgggac ggcatgattg aggacttgag ccaagcgcaa
    17821 aaaggcgaca tcgtcctgct gcacggctgc tgccacaatc ctaccggcat cgaccctacg
    17881 cccgaacaat gggaaacttt ggcaaaactt tctgccgaaa aaggctggtt gccgctgttt
    17941 gactttgcct accaaggctt cggcaatggt ttggaagaag atgcctacgg cttgcgcgtg
    18001 ttcttgaaac acaatacaga attgctgatt gccagctctt attccaaaaa cttcggtatg
    18061 tacaacgagc gcgtcggcgc gttcactttg gtggccgaag atgaagaaac agcagcccgc
    18121 gcccacagcc aagtcaaaac catcatccgt accttgtatt ccaacccggc ttcacacggt
    18181 gcgaacacca ttgcgctggt gttgaaaaat gatgatttga aagcacaatg gattgccgaa
    18241 ctcgatgaaa tgcgcggccg catcaaagcc atgcgccaaa aatttgtcgg gttgctcaaa
    18301 gccaaaggtg caagccaaaa ctttgatttc attatcaaac aaaacggtat gttctctttc
    18361 agcggcttga ctcccgaaca agtcgaccgc ctgaaaaacg agtttgccat ttatgccgtc
    18421 cgctccggcc gcatcaacgt cgccggcatt accgacgaca acatcgatta tctgtgcgaa
    18481 agcatcgtga aagtcttgta aaacaaaagc gcatacgaaa tgccgtctga accttaacca
    18541 gttcagacgg catctgtttt ttgttaaaaa acaataaacc ataacaattt caaacaagct
    18601 aaaaaacatc tataaaatca acccaatatg atgaactcaa ataaaaaata cgaatatcat
    18661 cattctcaaa ttcaaaattt gaacgccaca gtattatccg aagccacaaa aacctttccg
    18721 ccgtcattcc cgcgcaggcg ggaatccggg aacccaatgc tgcaagaatt gattagaaac
    18781 aactgaaacc gagcaaactg gattcccgcc tgcgcgggaa tgacgaaata aggcgagtaa
    18841 aactgatgaa acaaattgat taaaaacaat aacttatatt attttattta aattttcgat
    18901 aaaatcaatt ttccgtattc aatactgctc caatcaattc ccgcgcaccg tcgtccgcca
    18961 atttcttagc gacagcgcgt ccgagcgcgt cggcatattc ggcaggggct tgcgcgtccg
    19021 cctgcaacac aaccgaaccg tcggggtgtc cgaccagtcc gcgcaaggtc agcaagccgt
    19081 tttcttccgt gcaatatgcg gccagcggca cttggcagct tccgcccaaa gcgcgcgcca
    19141 aagctcgttc ggccgtaacg caggcgtgtg tggtatcgtg gtttagcggt tttaaaacct
    19201 catacaaatc ttcgcggtgc gcggcaattt cgatacccaa tgcgccttgt ccggcggcag
    19261 gcaggctgtc ggattccgac aaaatcatgc ggatgcgtcc gtccaatttc agacgctgca
    19321 aaccggcggc agccaagata attgcgtcgt attcgccgtt atcgagtttg gacaaacggg
    19381 tttgcacatt gccgcgcaaa ggtttgataa gcaaatgcgg atagcgcgca cgcaactggg
    19441 cttcgcggcg caggctggat gtgccgacaa ccgcgccttc gggcatttct tccaaacgcg
    19501 tgtattggtt ggacacaaat gcgtcaaacg gattggcgcg ttcgccgatg gccgcaagcg
    19561 cgaaaccttc aggcaaatcc atcggtacgt ctttaatcga atgcaccgcc aaatcggcgc
    19621 gcccgtcata taaagcctgt tccaactctt tgacaaacaa gcctttaccg ccgacttttg
    19681 acaaagtttt gtccaaaatc tgatcgccgc gcgtggtcat gcccaaaatc tcgacttcgc
    19741 aatcgggata cagagccttc agacggcctt ggatatgctt tgcctgccac atagcaagca
    19801 ggctttcgcg gctggcgatg acgagttttt tcgggttcat gttcaatgcg ttcaaagaat
    19861 ggaaacggcg tgaagtctat catattttga acgtttcttc agacggcacc gtcaggtcta
    19921 tgttatattt acacccacaa aacacgaaac ccgccacaat gaaacgcacc aaaaaatacc
    19981 ctttcttaac gttgcagcgg caacgtttcc atttgaactt tgaaaacgcc tcttccgccg
    20041 ccggcatccc agccgaacgc gatttctacc gctgggcgtg gtctgccttg aaaaatgaat
    20101 accgccgcgc cgacatcagc ctgattcttt tggacgaaga agaagcccgc gactacaacc
    20161 gcgactaccg gggcaaagat tacgccacca atgtcttgag tttcgcgctc aacgaaggcg
    20221 aaaccctgcc ctgccaagtt tcggaaaaac tgtacggcga tttgattatc tgcccgcaag
    20281 tggttttgaa agaagccgcc gaacaaggca aaacacccga gcagcatttt gcccacctga
    20341 ccatacacgg tactttacac ctgatgggct acgaccacat cgaagacgac gaagccgaaa
    20401 taatggaagc cgaagaaatc cgcctgatgc gggcggcagg cttccccaac ccctaccaag
    20461 aggacggaca ttaaaatgga cggcgcacaa ccgaaaacaa atttttttga acgcctgatt
    20521 gcccgactcg cccgcgaacc cgattccgcc gaagacgtat taaacctgct tcggcaggcg
    20581 cacgagcagg aagtatttga tgcggatacg cttttaaggt tggaaaaagt cctcgatttt
    20641 tccgatttgg aagtgcgcga cgcgatgatt acgcgcagcc gtatgaacgt tttaaaagaa
    20701 aacgacagca tcgagcgcat caccgcctac gttatcgata ccgcccattc gcgcttcccc
    20761 gtcatcggcg aagacaaaga cgaagttttg ggcattttgc acgccaaaga cctgctcaaa
    20821 tatatgttta accccgagca gttccacctc aaatccattc tccgccccgc cgtcttcgtc
    20881 cccgaaggca aatcgctgac cgccctttta aaagagttcc gcgaacagcg caaccatatg
    20941 gcgatcgtca tcgacgaata cggcggcaca tccggtttgg tcacctttga agacatcatc
    21001 gagcaaatcg tcggcgaaat cgaagacgaa tttgacgaag acgatagcgc cgacaatatc
    21061 cacgccgttt cttcagaacg ctggcgcatc cacgcggcta ccgaaatcga agacatcaac
    21121 accttcttcg gtacggaata cagcagcgaa gaagccgaca ccatcggcgg cttggtcatt
    21181 caagagttag ggcatctgcc cgtacgcggc gaaaaagtca tcatcggcag cctgcaattt
    21241 accgtcgcac gcgccgacaa ccgccgcctg catacgctga tggcgaccca cgtgaagtaa
    21301 gcagagcctg cccgcaccgc cgtttctgca cagtttagga tgacggtacg gacgttttct
    21361 gtttcaatcc gccccatccg ccaaacataa aaatgccgtc tgaaaccgga aaacaggttc
    21421 agacggcatt tcgcttatcc gcttattttt tgctcaaagt cagccccgtc caaccgaata
    21481 acagggtcaa agccaaactc aaatagcaga aaaaggcata cggcagatat tcccaaaccg
    21541 gcacgcccag cgcgtggctg atgaacacgc cgcatacgct ccacggtacg agcgggttaa
    21601 tcaccgtccc cgcatcttcc agcgtccgcg acagattgcg cgaatgcaga ccgagcttat
    21661 cgtaaacggg tttgaacgtt tcacccgaca acaaaatact caaatattgc tcgccgatca
    21721 ggaaattaac cccgaccgaa gtcatggcaa cgctgaatgt ggcacgtccg gcattcgtca
    21781 aaaagctgcg gacggcatcc agcagggaag gaatcgcgcc cagcgcaaac agcagcccgc
    21841 ccaaactcat cccgagaatc acgatggttt gcgtgaaaaa catactttcc aagccgccgc
    21901 gcgaaatgag tttggcgatg tctttaaacg cttcgccttc gagtttgtag ccgccgtaaa
    21961 accacgcgcc gagctgacgc agatcgggcg tgctgtgcag atacgttacg gcaacggcgg
    22021 caatgacggt aaagagcatg gcgaccacgg cattgacgcg catcaatgcc aaaacgacca
    22081 acagtgcaaa cggaatcagc gaatagccgt gcaccaatcc cgtggcttca agctggctgc
    22141 ggaaggattc gacgctgttc aaatcctgcg cggcgacact gggaagaagc cacagcatca
    22201 gtgccgcact gatgagccac gcgggaatgg tggtgtacat catatttttg atgtgctcaa
    22261 acaggtcgat gccgacgatg gacgcggaaa tgcccgtggt gtcggaaagc ggggacattt
    22321 tgtcgccgaa aaacgcaccg gaaacaatcg cgcccgccgt catcgccata tcggcatgaa
    22381 acgccgccgc catccccata aaggcaacgc cgacggtggc gcaggtggtc aggctgctgc
    22441 cgatggatac gccgatgacg gaacacagcg cgaaagcgga aaaataaaaa taagtcgggg
    22501 aaatcagccc gaaaccgtaa tacatcagcg tcggaatcgc accgctcatc atcagcgcgc
    22561 tgaccatcag cccgatgaag aaaaacaggt aaatcgcgcc cataccctga ttcaacgcgc
    22621 ctatcatgcc ctgctgcata tcgttgtatt tcaaaccgcg cgccaagccg tacaaaatca
    22681 gcacgacgat ggcggcaata atggacatat gcggcagcca ctccaatgaa atgatggtat
    22741 agcccatcgc ggcaatcaga gcgacgacga cggcaagtgc ctcaccgcgc ggcatatcga
    22801 gtaaggattt gaaagcgaac atgattcttt gcccgatttg atataaagtt aacggattct
    22861 aacacactat accgaatccg cccgacgttt tcagacggca ttttagcgtt ttacaaaagc
    22921 cggacagaaa caaaacggca attttctcaa tttaaattaa tttttttgaa atataatttt
    22981 ttattttttt gtttgttgac atataagact tttatggatt gaagtattaa aacctcggca
    23041 cacacgccgc aaacaccggt acgcaaaaat gccgtctgaa agatgttcag acgacatttc
    23101 tcaagaaaaa gattatttgt ccgccctata tccgtacacc gcaaaaaaca cgatatacag
    23161 ataacagatg acggaaacga caaacgaaga catcagggtg taagtatctg ccacccagcc
    23221 ctgcacgaca ggaacgaccg caccgccgac aatcgcggta cacagtacac cggaagccgc
    23281 gttggtaaat tttcccaatc ctttggttgc caaagagaaa atcgtcggga acataatcga
    23341 gttgaaaaaa ccgatggcaa gcagcgacca catcgccaca tccgcattgc cgctacccgt
    23401 cgccatcgcg acggcaagca gtacgaccgc agccgatgcg ttaaacgcca aataacggtt
    23461 gggcgcgaat ttcgccatca ccgccgaacc gaggaaacgt ccgaccatcg cgccgcccca
    23521 atagaacgac aggtaatgcg cggcagaagc atgatccagc cctttcagat aacccaatac
    23581 gttgaccatc aacgaaccga tagacacctc cgcgccgaca tagcagaaaa tgcctgccgt
    23641 accgaacacg agatgtttgt attgccatac gctggttttg ccgtcgtggt tgtgcgcgct
    23701 ttcctcggcg gcaattttgc gcgcgtcggg cagccggatc attttcacga aaacggcaag
    23761 gataatcagc agccccgcca gtcccaaata gggaatctgt acggaagaaa tctgttccgc
    23821 cttgctgacg gtttgggttg cgtccgccag aatcaggaac gcgccgattt gcggcgcaat
    23881 ggtcgtaccc aaagcgttaa acgcctgaac cagcgtcagt gttgccgatt ccttgccggg
    23941 tttcgccagc agggtaacat aaggattacc ggcgacctga agcagcgtta cgccggaagc
    24001 caaaataaac aacgcgccca aaaataccgc gtaagaatgg ctacccgcag caggataaaa
    24061 cagcaggcat ccgaccgccg tcagcagaaa gccgccgata acgccgtttt tgtagccgac
    24121 tttgccgaca aaagccccca tcgggatgga catcaccgca taggcggtaa agaaacagaa
    24181 ttggatcagc atcgcctgaa cgtaagacag gtcgaaaatt tctttcaaat gagggataag
    24241 gatgtcgttc atgcaggtaa taaaacccat catgaagaac agcgtggtca agacgaccaa
    24301 tggggacgta tgattgtttt gtgattgcgc agacatgatt tgcctttcgt ggtgtcggaa
    24361 tattacccgt ttcaagaaaa tagaaattga catttcttaa cggcaatgca aatcatactg
    24421 atatttgaac attttgcaaa ctttatcgga tgcaggcaat gcgccgatct gaaattcaag
    24481 cagcagaaat taatatttta cagttgcttg catagatttt aatagatttt aaaaaattcc
    24541 ggcggacttg caaaaaccgt tcccaataaa atgaaaatcc gccgccgtat ccggaaaatt
    24601 cggaaatgcc gcatcgtggc atcaaaaccg gagcggaaag aatgtcgtat gcccgacaaa
    24661 aaaggggcat acggctgtgc cgtatttttt gcaccgtcct ccgcccttcg gcgggggcgt
    24721 gaaaatatag tggattaaca aaaatcagga caaggcgac
//
LOCUS       DAXHTY010000020        23864 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXHTY010000020 DAXHTY010000000
VERSION     DAXHTY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23864
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.020"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..143)
                     /locus_tag="ACFNMK_000693"
     CDS             complement(<1..143)
                     /locus_tag="ACFNMK_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6364008.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLR"
     gene            316..1725
                     /gene="murC"
                     /locus_tag="ACFNMK_000694"
     CDS             316..1725
                     /gene="murC"
                     /locus_tag="ACFNMK_000694"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC6364009.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            1839..2753
                     /locus_tag="ACFNMK_000695"
     CDS             1839..2753
                     /locus_tag="ACFNMK_000695"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC6364010.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAESCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            2743..3471
                     /locus_tag="ACFNMK_000696"
     CDS             2743..3471
                     /locus_tag="ACFNMK_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC6364011.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKTLGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            3557..4801
                     /gene="ftsA"
                     /locus_tag="ACFNMK_000697"
     CDS             3557..4801
                     /gene="ftsA"
                     /locus_tag="ACFNMK_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC6364012.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREVGGGLLARLKRWIENSF"
     gene            4920..6098
                     /gene="ftsZ"
                     /locus_tag="ACFNMK_000698"
     CDS             4920..6098
                     /gene="ftsZ"
                     /locus_tag="ACFNMK_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC6364013.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            6237..7547
                     /locus_tag="ACFNMK_000699"
     CDS             6237..7547
                     /locus_tag="ACFNMK_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC6364014.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGVIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            8173..9048
                     /gene="prpB"
                     /locus_tag="ACFNMK_000700"
     CDS             8173..9048
                     /gene="prpB"
                     /locus_tag="ACFNMK_000700"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC6364015.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVVGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            9134..10288
                     /gene="prpC"
                     /locus_tag="ACFNMK_000701"
     CDS             9134..10288
                     /gene="prpC"
                     /locus_tag="ACFNMK_000701"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC6364016.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPEREGHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     EEETIGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            10421..10603
                     /locus_tag="ACFNMK_000702"
     CDS             10421..10603
                     /locus_tag="ACFNMK_000702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364017.1"
                     /translation="MDKFQSIINLTADAALFVQNKATEIATIGGCQAKGVREIASNAG
                     KTLLEKYLNSEQKDEI"
     gene            10703..11473
                     /locus_tag="ACFNMK_000703"
     CDS             10703..11473
                     /locus_tag="ACFNMK_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFC6364018.1"
                     /translation="MEELEENLECLKLAASNQILLATVEQFAFAYNIDNHGKIMRLVG
                     NFKGGGATKLENEDVSKVDKSSHSGIALGAHTEAPYHAATKIFNNHSPAPSSLVLTAR
                     YNPLSEPTTVIPLQPILEKLHYLDLLALTTENFDFTRSETFTSGKGTGGSKVSILEFN
                     NDGTIGVKYNSYRFTTNVHAPQSVKDALNRFNELINNSEQYKINLQPNTCMIINNTLA
                     LHCRDVIQDNRRLLIRIFGYKNNIEYISLEDDPVLAQG"
     gene            11479..12267
                     /locus_tag="ACFNMK_000704"
     CDS             11479..12267
                     /locus_tag="ACFNMK_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFC6364019.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPILLIFVALYFVFSPKLDGSKEGKARISFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIISMF"
     gene            12345..14951
                     /gene="acnD"
                     /locus_tag="ACFNMK_000705"
     CDS             12345..14951
                     /gene="acnD"
                     /locus_tag="ACFNMK_000705"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HFC6364020.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRRPGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLQRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGREIRLKDIWPT
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGKAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSLARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIAAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVIGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            15754..16923
                     /gene="prpF"
                     /locus_tag="ACFNMK_000706"
     CDS             15754..16923
                     /gene="prpF"
                     /locus_tag="ACFNMK_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HFC6364021.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            17337..18536
                     /locus_tag="ACFNMK_000707"
     CDS             17337..18536
                     /locus_tag="ACFNMK_000707"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6364022.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            18643..19263
                     /locus_tag="ACFNMK_000708"
     CDS             18643..19263
                     /locus_tag="ACFNMK_000708"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6364023.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(19390..19833)
                     /locus_tag="ACFNMK_000709"
     CDS             complement(19390..19833)
                     /locus_tag="ACFNMK_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC6364024.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            20007..20375
                     /locus_tag="ACFNMK_000710"
     CDS             20007..20375
                     /locus_tag="ACFNMK_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6364025.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            20420..21574
                     /locus_tag="ACFNMK_000711"
     CDS             20420..21574
                     /locus_tag="ACFNMK_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6364026.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(21631..22503)
                     /locus_tag="ACFNMK_000712"
     CDS             complement(21631..22503)
                     /locus_tag="ACFNMK_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC6364027.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWRVVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            22588..23400
                     /locus_tag="ACFNMK_000713"
     CDS             22588..23400
                     /locus_tag="ACFNMK_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC6364028.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         5664 a         6052 c         6516 g         5632 t
ORIGIN      
        1 tctctaaggt aacgggttct ttgacggttc aggtattgtt cgatcggctg ccaatcaatc
       61 acttggtcca acttcaatag cgggaaacgg ttgatgtgtt tggcaatcat ggcttgcgcg
      121 gtttgccgga agaaggtgct catggaaaat cccctaaatg tcttggtggg aatttagggg
      181 attttggggg attttgcaaa ggtctcatcc tgtatgccga aacatctgta atttacaaga
      241 aaactatggc gtagaagcgg atagcccttt aaaataacgc ctttacgcat cgaaaatcca
      301 ccggaacgca acattatgat gaaaaatcga gttaccaaca tccattttgt cggtatcggc
      361 ggcgtcggca tgagcggtat cgccgaagtc ttgcacaatt tgggttttaa agtttccggt
      421 tcggatcagg cgcgaaatgc cgctaccgag catttgggca gcctgggcat tcaagtttat
      481 cccggccata ccgcagaaca cgttaacggc gcggatgtcg tcgttacctc taccgccgtc
      541 aaaaaagaaa atcccgaagt tgtcgctgcg ttggagcagc aaattcccgt tattccgcgc
      601 gccctgatgt tggcggagtt gatgcgcttc cgtgacggca tcgccattgc cggcacgcac
      661 ggcaaaacca cgaccaccag cctgaccgcc tccatcctcg gcgcggcagg acttgacccg
      721 actttcgtta tcggcggcaa actcaacgcc gcaggcacca acgcccgctt gggcaaaggc
      781 gaatacatcg ttgccgaagc cgacgagtcg gatgcatcct ttctgcacct gacaccgatt
      841 atgtccgtcg ttaccaatat cgacgaagac catatggata cctacgggca cagcgtcgaa
      901 aaactgcatc aggcgtttat cgatttcatc caccgtatgc ccttctacgg caaagccttt
      961 ttgtgtattg acagcgaaca cgtccgcgcg attttgccca aagtgagcaa accttatgct
     1021 acttacggtt tggacgatac cgccgacatc tacgccaccg acatcgaaaa cgtcggcgcg
     1081 caaatgaaat tcaccgtcca tgttcaaatg aaaggacatg agcaggggtc gtttgaagtc
     1141 gtgctgaata tgcccggcag acacaacgtg ctgaacgcat tggcagccat cggcgtggcg
     1201 ctggaagtcg gcgcatcggt tgaagcgatc caaaaaggct tgctcggctt tgaaggcgtc
     1261 ggccgccgct tccaaaaata cggcgacatc aagttgccaa acggcgggac cgcgctcttg
     1321 gtggacgact acggacacca ccccgtcgaa atggcggcga cccttgccgc cgcacgcggc
     1381 gcgtatccgg aaaaacgttt ggtactcgcc ttccagccgc accgctatac ccgcacgcgc
     1441 gatttgtttg aagactttac caaagtcctc aataccgttg acgcgctggt gctgaccgaa
     1501 gtttatgccg ccggtgaaga gccgattgcc gccgccgatt cccgcgctct tgcccgcgcc
     1561 atccgcgtgt tgggcaaact cgagccgatt tactgcgaaa acgttgccga tctgcccgaa
     1621 atgctgttga acgttttgca ggacggcgac atcgtgttga atatgggcgc gggaagcatc
     1681 aaccgcgttc ccgccgcgct actggaattg tcgaaacaga tttgaggcac accctcctga
     1741 cagacggaac atcatataaa ggtcgtctga aaccgcaaat caggtttcag acgacctctg
     1801 gcaacaagca taaagcaatc aggaaagaac aaaaaacaat gcagaatttt ggcaaagtgg
     1861 ccgtattgat gggcggtttt tccagcgaac gagaaatctc gctggacagc ggcaccgcca
     1921 ttttgaacgc tttaaaaagc aaaggcatag acgcatacgc cttcgatccc aaggaaaccc
     1981 cattgtctga attgaaggca caaggttttc agacggcatt caacatcctt cacggtactt
     2041 acggcgaaga cggggcggtt caaggcgcgt tggaactgtt gggcattccc tataccggca
     2101 gcggtgtcgc cgcctccgcc atcggcatgg acaaataccg ctgcaaactg atttggcagg
     2161 cattggggct gcccgttccc gagttcgccg tcctgcacga cgacactgat ttcgatgccg
     2221 tcgaagaaaa attgggcctg ccgatgtttg tgaaaccggc ggccgaaggc agcagcgtcg
     2281 gtgtggtaaa agtcaaagaa aaaggccgtc tgaaaagcgt ttacgaagaa ttgaaacacc
     2341 ttcagggcga aatcattgcc gaacgtttta tcggcggcgg cgaatattcc tgccccgtcc
     2401 tgaacggcaa agggctgccc ggcatacaca tcattcccgc aaccgagttt tacgactacg
     2461 aagccaagta caaccgagac gacaccattt atcaatgtcc ttcggaagat ttgaccgaag
     2521 ccgaagaaag cctgatgcgc gaactggcgg ttcgcggcgc gcaggcaatc ggtgcggaaa
     2581 gctgcgtgcg cgtcgatttc ctcaaagata ccgacggcaa actctatctg ttggaaatca
     2641 acaccctgcc cggtatgacg agccatagtt tagtaccgaa atccgctgcc gttacgggcg
     2701 tgggttttgc cgatttatgt attgaaattt tgaagaccgc acatgtggga taatgccgaa
     2761 gcgatggaac ggctgacgcg ctggctgctt gtcatgatgg cgatgctgct tgctgcgtcc
     2821 gggctggttt ggttttacaa ttcgaatcat ctgcccgtca agcaggtgtc gctgaagggc
     2881 aacctggttt attccgataa gaagacattg ggcagtttgg cgaaagaata catccatggg
     2941 aatattttga ggacggacat caatggcgca caggaggcct accgccggta tccgtggatt
     3001 gcgtcggtca tggtgcgccg ccgttttccc gacacggttg aggtcgtcct gaccgagcgc
     3061 aagccggtcg cgcgttgggg cgaccatgcc ttggtggacg gcgaaggcaa tgtttttgaa
     3121 gcccgcttgg acagacccgg aatgccggta ttcagaggcg cggaaggaac gtctgccgaa
     3181 atgctccgcc gttatgacga attttcgact gttttggcaa aacagggttt gggcatcaaa
     3241 gagatgacct atacggcacg ttcggcgtgg attgtcgttt tggacaacgg catcaccgtc
     3301 aggctcggac gggaaaacga gatgaaacgc ctccggcttt ttaccgaagc gtggcagcat
     3361 ctgttgcgta aaaataaaaa tcggttatcc tatgtggata tgaggtataa ggacggattt
     3421 tcagtccgct atgcttccga cggtttaccc gaaaaagaat ccgaagaata gtgggaacag
     3481 gtatcggaca gattacggcc gtgccgtctg aaacggtgcg acgcaaattt caatcagttt
     3541 taagagcaga cgaacaatgg aacagcagca aagatacatc agcgtactgg atatcggtac
     3601 gtctaaagtc ctcgcactga tcggggaagt tcaagatgac gacaaaatca acatcgtcgg
     3661 tttggggcag gctccttcac ggggcttgcg cgcgggcatg gtaaccaata tcgatgccac
     3721 cgtccaagcc atcaggcagg cggtcaatga tgccgagctg atggcggata ccaaaattac
     3781 tcacgttacc acaggtatcg caggcaacca catccgcagt ctcaattcgc aaggtgtggt
     3841 taaaattaaa gatggggaag tcacgcaggc agacatcgat cgcgccattg aaacggcaaa
     3901 ggcaatcaat atcccgcccg atcaaaaaat tctcgatgcc gtggttcaag actacattat
     3961 tgacacccaa cttggcgtga gggagcccat cggtatgagc ggtgtgcgtc tggatacgcg
     4021 ggtgcacatc attaccggtg caagtacggc agtgcagaat gtccaaaaat gtatcgagcg
     4081 gtgcggtttg aaaagcgatc agatcatgct tcagccgttg gcaagcgggc aggcggtgct
     4141 gactgaagat gaaaaagacc tcggcgtatg cgtcatcgac attggtggcg gaacgaccga
     4201 tattgccgtt tatatgaacg gtgccatccg ccatacgtcc gtcattccgg ccggtggtaa
     4261 tctgattacc aaagatttgt ccaaatcgtt gagaacacct ctcgatgccg ccgagtacat
     4321 taaaatccat tatggcgtgg catcatgcga tacggaaggc ttgggtgaga tgattgaagt
     4381 tccgggcgtg ggtgaccgga catcgcgtca ggtttccagt aaggttttgg cagcaatcat
     4441 cagcgcacgt attcaggaga tttttggcgt agtgctgggc gagctgcaaa aatcgggttt
     4501 ccccaaagaa gtgctgaatg cgggtatcgt tctgaccggc ggtgtgtcca tgatgaccgg
     4561 gattgtggaa tttgccgaaa aaatcttcga tttgcctgta cgcaccggtg cgccccaaga
     4621 aatgggcggt ttgtccgacc gcgtccgcac accgcgcttt tctaccgcca tcgggctgct
     4681 tcatgcagca tgcaagctgg aaggaaactt gccgcagccg gaaaacggtg cagtgcaaga
     4741 gagggaagtg ggcggcggtt tgttggcaag attgaaacgg tggattgaaa acagcttctg
     4801 aacaggtgga ttgccgtttg acaggtgaga agtattttgc cagcagcaag atacttctta
     4861 tataatgaat aataatttat ttaaaccgtc ctctgaatgg ggcgagcagg agtttttgaa
     4921 tggaatttgt ttacgacgtg gcagaatcgg cagtcagccc tgcggtgatt aaagtaatcg
     4981 gcttgggcgg cggcggttgc aatgcaatca ataacatggt tgccaacaat gtgcgcggtg
     5041 tggagtttat cagtgccaat acggatgcgc agtctctggc aaaaaaccat gcggcgaaga
     5101 gaatccagtt gggtacgaat ctgacacgcg gtttgggcgc gggcgcgaat cccgatatcg
     5161 gccgtgcggc agcccaggaa gaccgggaag ccattgaaga agccattcgc ggtgcgaata
     5221 tgctgtttat cacgaccggt atgggcggcg gtaccggtac cggttccgcg ccggttgttg
     5281 ctgagattgc caagtctttg ggcattctga ccgttgccgt ggttacccga ccgttcgcat
     5341 atgaaggtaa gcgcgtccat gtcgcacagg cagggttgga acagttgaaa gaacacgtcg
     5401 attcgctgat catcatcccg aacgacaaac tgatgactgc attgggtgaa gacgtaacga
     5461 tgcgcgaagc cttccgtgcc gccgacaatg tattgcgcga tgcggtcgca ggcatttccg
     5521 aagtggtaac ttgcccgagc gaaatcatca acctcgactt tgccgacgtg aaaaccgtga
     5581 tgagcaaccg cggtatcgct atgatgggtt cgggttatgc ccaaggtatc gaccgtgcgc
     5641 gtatggcgac cgaccaggcc atttccagtc cgctgctgga cgatgtaacc ttggacggag
     5701 cgcgcggtgt gctggtcaat attacgactg ctccgggttg cttgaaaatg tccgagttgt
     5761 ccgaagtcat gaaaatcgtc aaccaaagcg cgcatcccga tttggaatgc aaattcggtg
     5821 cggctgaaga cgagaccatg agcgaagatg ccatccggat taccattatc gctaccggtc
     5881 tgaaagaaaa aggcgcggtc gattttgttc cggcaaggga ggtagaagcg gttgccccgt
     5941 ccaaacagga gcaaagccat aatgtcgaag gtatgatccg caccaatcgc ggtatccgca
     6001 cgatgaacct taccgctgcg gatttcgaca atcagtccgt acttgacgac tttgaaatcc
     6061 ctgcgatttt gcgtcgtcaa cacaattcag acaaataatg tgctgtttgc ccgtaaacct
     6121 gctgcctccc gaatcggttt gtccggtttg ggaggtatgt ttttcaagat gttgcaattt
     6181 cgtacggttt gcggtcggcg gattcagatt tttccacttg atacagactt tcagatatgg
     6241 acacttcaaa acaaacactg ttggacggga tttttaagct gaaggcaaac ggtacgacgg
     6301 tgcgtaccga gttgatggcg ggtttgacaa cttttttgac gatgtgctac atcgttatcg
     6361 tcaaccccct gattttgggc gagaccggca tggatatggg ggcggtattc gtcgctacct
     6421 gtatcgcgtc tgccatcggc tgttttgtta tgggttttgt cggcaactat ccgattgcac
     6481 tcgcaccggg gatggggctg aatgcctatt tcacctttgc cgtcgttaag ggtatgggcg
     6541 tgccttggca ggttgcgttg ggtgcggtgt tcatctccgg tctgattttc atcctgttca
     6601 gcttttttaa agtcagggaa atgttggtca acgcactgcc tatgggtttg aaaatgtcga
     6661 ttgctgccgg tatcggtttg tttttggcac tgatttccct gaaaggcgca ggcattatcg
     6721 ttgccaatcc ggcaaccttg gtcggcttgg gcgatattca tcagccgtcc gcgttgttgg
     6781 cactgttcgg ttttgctatg gtggtcgtat tgggacattt ccgcgttcaa ggcgcaatca
     6841 tcatcaccat tttgacgatt accgtcattg ccagcctgat gggtttgaat gaattccacg
     6901 gggtaatagg cgaagtgccg agcattgcgc cgacttttat gcagatggat tttgaaggcc
     6961 tgtttaccgt cagcatggtc agtgtgattt tcgtcttctt cttggtcgat ctatttgaca
     7021 gtaccggaac gctggtcggc atatcccacc gtgccgggct gctggtggac ggtaagctgc
     7081 cccgcctgaa acgcgcactg cttgcagact ctaccgccat tgtggcaggt gcggctttgg
     7141 gtacttcttc caccacgcct tatgtggaaa gcgcggcggg cgtatcggca ggcggacgga
     7201 ccggcctgac ggcggttacc gtcggcgtat tgatgctcgc ctgcctgatg ttttcacctt
     7261 tggcgaaaag tgttccggca tttgccaccg cgcccgccct gctttatgtc ggcacgcaga
     7321 tgctccgcag tgcgagggat attgattggg acgatatgac ggaagccgca cccgcattcc
     7381 tgaccattgt cttcatgccg tttacctatt cgattgcaga cggcatcgcc ttcggcttca
     7441 tcagctatgc cgtggttaaa cttttatgcc gccgcaccaa agacgttccg cctatggtat
     7501 ggattgttgc cgtattgtgg gcactgaaat tctggtattt gggctgattg attcgatatt
     7561 aaaaatgccg tctgaaaggt tttcagacgg cattttgttt gccgatatat ttaattttta
     7621 ttaaattata taaaaatcaa atacataata aaatacatcg gattgcttaa aaataataca
     7681 ttgtttttat gtataaaata ttttataagt tttcaggatt ttgattatcg aaaatttttc
     7741 ttgatttcct gacaatttta ttgaaacaaa taattcaaaa ttaatctagt ttaatcatgg
     7801 aattaaaata aaatattaaa attatgtaat gagtctcctt aaaaatgttt gacattttca
     7861 gtcttgtgtt ttagattagc gaaaaataaa accacacaat actacaaagg aacattacta
     7921 tgaaaccaat tcagatgttt tccccttttc tgaataatcc ccttgttttc ttcttgtctg
     7981 cggttttgcc gcataattcc gaacggtctg ctgtttttct ttgattcgtt ttaaatatca
     8041 ataagataat ttttcccata tatttttaat gattggattg ggatgcccga cgcgtcggat
     8101 ggctgtgttt tgccgtccga atgtgatgga agcctgtcca tactgaaaaa aagtctataa
     8161 aggagaaata tgatgagtca acactctgcc ggagcacgtt tccgccaagc cgtgaaagaa
     8221 tcgaatccgc ttgccgtcgt cggttgcgtc aatgcttact ttgcacgatt ggccacccaa
     8281 agcggtttca aagccatcta tctgtccggc ggcggcgtgg cagcctgttc ttgcggtatc
     8341 cctgatttgg gcattaccac aatggaagat gtgctgatcg acgcacgacg cattacggac
     8401 aacgtggata cgcctttgct ggtggacatc gatgtgggtt ggggcggtgc attcaatatt
     8461 gcccgtacca ttcgcaactt tgaacgcgcc ggtgttgcag cggttcacat cgaagatcag
     8521 gtagcgcaaa aacgctgcgg ccaccgtccg aacaaagcca ttgtatctaa agatgaaatg
     8581 gtcgaccgta tcaaagctgc cgtagatgcg cgcgttgatg agaacttcgt gattatggcg
     8641 cgtaccgatg cgctggcggt agaaggtttg gatgccgcta tcgaacgcgc ccaagcttgt
     8701 gtcgaagccg gtgcggacat gattttccct gaagccatga ccgatttgaa catgtaccgc
     8761 caatttgcag atgcggtgaa agtgcccgtg ttggcgaaca ttaccgagtt tggttccact
     8821 ccgctttata cccaaagcga gctggctgaa aacggcgtgt cgctggtgct gtatccgctg
     8881 tcatcgttcc gtgcagcaag caaagccgct ctgaatgttt acgaagcgat tatgcgcgat
     8941 ggcactcagg cggcggtggt ggacagtatg caaacccgtg ccgagctgta cgagcatctg
     9001 aactatcatg ccttcgagca aaaactggat aaattgtttc aaaaatgatt taccgctttc
     9061 agactgcctt tcaacaaatc cgcatcggtc gtctgaaaac ccgaaaccca taaaaacaca
     9121 aaggagaaat accatgactg aaactactca aaccccgacc ctcaaaccta aaaaatccgt
     9181 tgcgctttct ggcgttgcgg ccggtaatac cgctttgtgt accgttggcc gtaccggcaa
     9241 cgatttgagc tatcgcggtt acgacattct ggatttggca caaaaatgtg agtttgaaga
     9301 agttgcccac ctgctgattc acggccattt acccaacaaa ttcgagctgg ccgcttataa
     9361 agccaagctc aaatccatgc gcggcctgcc tatccgtgtg attaaagttt tggaaagcct
     9421 gcctgcacat acccatccga tggacgtgat gcgtaccggc gtatccatgc tgggctgtgt
     9481 tcatcctgaa cgtgaaggcc atccggaaag cgaagcgcgc gacattgccg acaaactgat
     9541 cgccagcctc ggcagtatcc tcttgtactg gtatcaatat tcgcacaacg gcaaacgcat
     9601 tgaagttgaa agcgaagaag agaccatcgg cggtcatttc ctgcacctgt tgcacggcaa
     9661 acgcccaagc gaatcacaca tcaaagccat gcacgtttca ctgattctgt atgccgaaca
     9721 cgagttcaac gcttctacct ttaccgcccg cgtgatcgcc ggtacaggct ctgatatgta
     9781 ctccagcatt accggagcaa tcggcgcgtt gaaaggtccg aaacacggcg gcgcgaacga
     9841 agtggcttac gatattcaaa aacgctaccg caatgccgac gaagccgaag ccgacatccg
     9901 cgaacgcatc ggccgcaaag aaatcgtgat cggtttcggt catccggtgt acaccatttc
     9961 cgaccctcgc aacgttgtca ttaaagaagt ggcacgcggt ttgagcaaag aaaccggcga
    10021 tatgcgcctc tttgacattg ccgaacgttt ggaaagcgtg atgtgggaag agaaaaaaat
    10081 gttcccgaat ctggactggt tctctgccgt ttcctaccaa aaattgggcg taccgaccgc
    10141 tatgttcaca ccgctgttcg taatttcccg tacaaccggt tggagcgcac acgttctcga
    10201 gcaacgcaaa gacggcaaaa tcatccgtcc gagcgcaaac tacacaggtc cagaagattt
    10261 ggcgtttgtg gagattgaag aacgataatt gaagaatgca atagcagttt gttctttatt
    10321 tcggtatgca aagctgagga tttcagacga ccttgcctta ttggaaaggt tgtctgaaat
    10381 aagtttaaaa atttaaccta ataaaaaggg tatgtattaa atggataagt ttcaatcaat
    10441 tattaattta actgctgatg ctgctttgtt tgttcaaaat aaagcaacgg aaatcgcaac
    10501 aataggtggc tgtcaagcaa agggagtaag agaaattgca agtaatgcag ggaaaacctt
    10561 attggaaaaa tatttaaact cagaacaaaa ggatgaaatc tagaaattca ttcatcaaaa
    10621 aacttgtgta gttattttta ataatcttta tgataaaaac attgataatt caataattag
    10681 cattccaagt tctttaccaa aaatggaaga gcttgaagaa aacttagaat gtttgaaatt
    10741 ggctgcatct aatcagattt tattggcaac agtagagcaa tttgcatttg cttataatat
    10801 tgataatcat gggaaaataa tgagacttgt tggtaatttc aagggaggtg gagcaacaaa
    10861 acttgaaaat gaagatgtta gcaaagtaga taaaagttca cattcaggaa ttgcattagg
    10921 ggcgcatact gaagctcctt accatgcggc aacaaagatt tttaataatc actcccccgc
    10981 accatcatct cttgttttaa ctgcaagata caatccgcta tcagaaccga caacagtaat
    11041 tccattacaa cctattttgg aaaagttgca ttatttggat ttattggcat taacgacaga
    11101 aaattttgac tttactcgta gtgagacgtt tacgtcagga aaaggaacag ggggaagtaa
    11161 agtatctata cttgaattta ataacgatgg aactattggt gttaaatata actcttatag
    11221 atttactact aatgtgcatg caccccaatc ggttaaagat gctttgaata gatttaatga
    11281 attaatcaac aactctgaac aatataaaat caacttacaa ccaaacactt gtatgatcat
    11341 taacaacacg ttggctttgc attgccgtga tgttattcaa gataacagaa gattgttaat
    11401 tcgtattttt ggatataaaa ataatattga gtatatttct ttagaagatg atcctgtatt
    11461 ggctcaagga taagaaacat ggaagattta tatataatac tcgctttggg tttggttgcg
    11521 atgattgccg gatttatcga tgcgattgcg ggtgggggtg gtttgattac gctgcctgca
    11581 ctcttgttgg caggtattcc tcccgtgtcg gcaattgcca ccaacaagct gcaagcagcc
    11641 gctgctacgt tttcggctac ggtttctttt gcacgcaaag gtttgattga ttggaagaaa
    11701 ggtctcccga ttgcggcagc atcgtttgca ggcggcgtgg tcggtgcatt atcggtcagc
    11761 ttggtttcca aagatattct gctggcggtc gtgccgattt tgttgatatt tgtcgcgctg
    11821 tattttgtgt tttcgcccaa gctcgacggc agtaaggaag gcaaagccag aatatctttt
    11881 tttctattcg ggctgacggt tgcaccgctt ttgggttttt acgacggtgt gttcggaccg
    11941 ggtgtcggct cgttttttct gattgccttt attgttttgc tcggctgcaa gctgttgaac
    12001 gcgatgtctt acaccaaatt ggcgaacgtt gcctgcaatc ttggttcgct atcggtattc
    12061 ctgctgcacg gttcgattat tttcccgatt gcggcaacga tggcggtcgg tgcgtttgtc
    12121 ggtgcgaatt taggtgcgag atttgccgtc cgcttcggtt cgaagctgat taagccgctg
    12181 ctgattgtca tcagcatttc gatggctgtg aaattgttga tagacgagag aaatccgctg
    12241 tatcagatga ttatttcgat gttttaaacc ctttcagacg accccttcaa aacgtcggct
    12301 gaaacctcaa accacaagaa aaacagatcc ccaggagaac cgacatggct gccaaccaac
    12361 gttaccgcaa accgctgccc ggtacggatt tggaatacta cgacgcgcgt gcggcgtgtg
    12421 aggacatcaa gcccggctct tacgacaagc tgccttacac gagccgcatt ttggcggaga
    12481 atttggtcaa ccgcgcggac aaagtcgatt tgccgacgct gcaaagctgg ctgggtcagc
    12541 tgattgaggg aaaacaggaa atcgactttc cttggtatcc ggcgcgggtg gtgtgccacg
    12601 atattctggg gcagaccgcg ttggtggatt tggcaggtct gcgcgatgcg attgccgaaa
    12661 aaggcggcga tcctgccaaa gtgaatccgg tggtgcaaac ccagctcatc gtcgaccact
    12721 ctctggccgt tgaatgcggc ggctacgacc ccgatgcctt ccgcaaaaac cgccaaatcg
    12781 aagacagacg taacgaagac cgtttccact tcatcaactg gacaaaaacc gcatttgaaa
    12841 atgtggacgt gattccggcg ggcaacggca tcatgcacca aatcaatcta gaaaaaatgt
    12901 cgcccgtcgt ccaagtcaaa aacggcgtgg cgttccccga tacctgcgtc ggtactgact
    12961 cacatacgcc gcacgtcgat tcattgggcg tgatttccgt gggcgtgggc ggattggaag
    13021 cggaaaccgt aatgctggga cgcgcgtcca tgatgcgcct gcccgatatt gtcggcgttg
    13081 agctgaccgg caaacgccgg ccgggcatta ctgccaccga catcgtgttg gcactgactg
    13141 agttcttgcg caaagaacgc gtggtcgggg catttgtcga attcttcggc gaaggtgcga
    13201 gaagcctgtc catcggcgac cgcgcaacta tttccaatat gacccctgaa ttcggcgcga
    13261 ctgccgcgat gttcgctatt gatgagcaaa ccattgatta tttgaaactg accggacgcg
    13321 acgacgcgca ggtgaaattg gtggaaacct acgccaaaac cgcaggctta tgggcagatg
    13381 ccttgaaaac cgccgtttat ccgcgcgttt tgaaatttga tttgagcagc gtaacgcgca
    13441 atatggcagg cccaagtaac ccgcatgccc gttttgcgac cgccgatttg gcggcgaaag
    13501 ggctggcgaa gccttacgaa gagccttcag acggccaaat gcctgacggt gcagtgatta
    13561 ttgccgcgat tacttcgtgt accaatactt ccaatccgcg caacgttgtc gccgctgcgc
    13621 tgttggcacg caatgccaac cgcctcggct tgcaacgcaa accttgggtg aaatcttcgt
    13681 ttgccccggg ttcaaaagta gccgaaatct atttgaaaga agcaggcctg ctgcctgaaa
    13741 tggaaaaact cggcttcggt atcgttgcct tcgcatgtac cacctgtaac ggcatgagcg
    13801 gcgcgctcga cccgaaaatc cagaaagaaa tcatcgaccg cgatttgtac gccaccgccg
    13861 tactgtcagg caaccgcaac ttcgacggcc gtattcatcc gtatgcgaaa caggctttcc
    13921 tcgcttcgcc cccgttggtc gttgcttacg cgctggcagg cagcatccgt ttcgatattg
    13981 aaaacgatgt actcggcgtt gcagacggcc gcgaaatccg cctgaaagac atctggccga
    14041 ccgacgaaga aatcgatgcc gtcgttgctg aatatgtgaa accgcagcag ttccgcgatg
    14101 tgtatgtacc gatgttcgac accggcaaag cgcaaaaagc acccagtccg ctgtacgatt
    14161 ggcgtccgat gtccacctac atccgccgtc cgccttactg ggaaggcgcg ctggcagggg
    14221 aacgcacatt aagaggtatg cgtccgctgg cgattttgcc cgacaacatc accaccgacc
    14281 acctctcgcc gtccaatgcg attttggccg tcagtgccgc aggcgagtat ttggcgaaaa
    14341 tgggtttgcc tgaagaagac ttcaactctt acgcaaccca ccgcggcgac cacttgaccg
    14401 cccaacgcgc taccttcgcc aatccgaaac tgtttaacga aatggtgaaa aacgaagacg
    14461 gcagcgtgcg ccaaggctcg ctcgcccgcg tcgaacccga aggcgaaacc atgcgcatgt
    14521 gggaagccat cgaaacctat atgaaccgca aacagccgct catcatcatt gccggtgcgg
    14581 actatggtca aggctcaagc cgcgactggg cggcgaaggg cgtgcggctc gcgggagtgg
    14641 aagccatcgc cgccgaaggt ttcgagcgca tccaccgcac caatctcatc ggcatgggcg
    14701 tcttgccgct gcaattcaaa cccggcacca accgccacac cctgcaactg gacggcacgg
    14761 aaacctacga cgttatcggc gaacgtacac cgcgctgcga cctgaccctc gtgattcacc
    14821 gtaaaaacgg agaaaccgtc gaagttcccg ttacctgccg cctcgatact gcagaagaag
    14881 tattggtata tgaagccggc ggcgtgttgc aacggtttgc acaggatttt ttggaaggga
    14941 acgcggctta gaggtcgtct gaaaagcaag acgtagcgtg ggtcgggttc aacattttgc
    15001 tcattcacgt aattctcgat atggcaggca tctactgtaa atcgtcattc ccgcgcaggc
    15061 gggaatccag aaagtggaat tgaggaaacc ttatttatcc gatgagtttc tgtgcggata
    15121 aatctggatt cccgcctgcg cgggaatgac ggggtttaat aatctgccgt atcacaacac
    15181 agtagccgta gattgtggcg aaccccgaca gtttgcggaa tcaaacggct ttgtcggagt
    15241 ggcagcctaa tgtacttctg gaaagtgggt gtagcgtggg ctttgctcgc gaaataaagg
    15301 ctgaattgac atggtataga ggattaacaa aaatcgggac aaggcggcga agccgcagac
    15361 agtacagata gtacggaacc gattcacttg gtgcttcagc accttagaga atcgttctct
    15421 ttgagctaag gcgagccaac gctgtactgg tttttgttaa tccactataa atttaatcca
    15481 ctatactgta aatcgtcatt cccgcgcagg cgggaatcca gaaagtggaa ttgaggaaac
    15541 ctttttatcc gatgagtttc tgtgcggata aatctggatt cccgcctgcg cgggaatgac
    15601 ggggtttaat aatctgccgt atcacaacac agtagccgta gattggggcg aaccccgaca
    15661 gtttgcggaa tcaaacggct ttggtcggag tggcagccta atccactata aaaatcgtgg
    15721 gcagagccca cgctacataa ggagaatcta gaaatgccgc aaattaaaat tcccgccgtt
    15781 tactaccgtg gcggtacatc aaaaggcgtg tttttcaaac gttccgacct gcccgaggcg
    15841 gcgcgggaag cgggaagcgc acgcgacaaa atcctcttgc gcgtactcgg cagcccggat
    15901 ccctacggca agcagataga cggtttgggc aacgccagct cgtccaccag caaggcggtg
    15961 attttggaca agtccgaacg cgccgatcac gatgtcgatt accttttcgg gcaagtttcc
    16021 atcgacaaac cttttgtcga ttggagtggc aactgcggca acctcaccgc cgccgtgggc
    16081 gcatttgcca tcgagcaagg cttggtcgat aaaggcaaga ttccttcaga cggcatctgc
    16141 acggtcaaaa tctggcagaa aaacatcggc aaaaccatta ttgcccatgt accgatgcaa
    16201 aacggcgcag ttttggaaac aggcgatttt gagctcgacg gcgtaacgtt cccggcagcc
    16261 gaagtacaaa tcgaatttct tgatccagcc gacggtgaag gcagtatgtt cccaaccggc
    16321 aatttggtcg atgaaattga tgtgccgaat ataggccgtt tgaaagccac gctcatcaac
    16381 gcgggcattc cgaccgtttt cctgaatgcc gccgacttgg gctacacggg caaagagttg
    16441 caagacgaca tcaacaacga tgccgcggct ttggaaaaat tcgagaaaat ccgcgcttac
    16501 ggtgcgctga aaatgggtct gatcagcgac gtatccgaag ctgccgctcg cgcgcacacg
    16561 ccgaaagtcg ccttcgtcgc gcccgccgcc gattacaccg cctccagtgg caaaaccgtg
    16621 aatgccgccg acatcgattt gctggtacgc gccctgagca tgggcaaatt gcaccacgcg
    16681 atgatgggta ccgcctctgt tgccattgcg accgccgccg ccgtgcccgg tacgctggtc
    16741 aaccttgccg caggcggcgg aacgcgtaaa gaagtgcgct tcgggcatcc ttccggcaca
    16801 ttgcgcgtcg gtgcagccgc cgaatgtcag gacggacaat ggacggccac caaagcggtt
    16861 atgagccgca gcgcacgcgt gatgatggaa ggttgggtca gggtgccgga agattgtttt
    16921 taaattgacg tagcatgggt ttgcccgcga gccataaaca ggtcgtctga aaaacaagta
    16981 aacatcaaat cactgaccat tcctttccct tgccctgtgg cggaaggcgg caaatcacaa
    17041 ggaagaacac ggaaaccccg ataaaagaca gcttcccgta ttaccgtcat tcccgcgcag
    17101 gcgggaatct agacctgtca atatggagga ttggtagggg aaaacaggtt tcgtgagttc
    17161 tacattctgg attcccgcca cagcctgtcc tcgcgtaggc ggggacggaa taacgataga
    17221 aaatgcggca tacgctttgc ccaaagaggc cgtctgaaac accttgcgcc tgatgtctgc
    17281 ctttttcaga cgaccccaca ccaaaaaaac aaccacaaac tacaaggaga aacatcatgt
    17341 ccgaccaact catcctcgtt ctgaactgcg gcagttcatc gctcaaaggc gccgttatcg
    17401 accgaaaaag cggcagcgtc gtcctaagct gcctcggcga acgcctgacc acgcccgaag
    17461 ccgtcattac gttcaacaaa gacggcaaca aacgccaagt tcccctgagc ggccgaaatt
    17521 gccacgccgg cgcggtgggt atgcttttga acgaactgga aaaacacggt ctgcacgacc
    17581 gcatcaaagc catcggccac cgcatcgccc acggcggcga aaaatacagc gagtctgttt
    17641 tgatcgacca ggccgtaatg gacgaactca atgcctgcat tccgcttgcg ccgctgcaca
    17701 accccgccaa catcagcggc atccttgccg cacaggaaca tttccccggt ctgcccaatg
    17761 tcggcgtgat ggatacttcg ttccaccaaa ccatgccgga gcgtgcctac acttatgccg
    17821 tgccgcgcga gttgcgtaaa aaatacgctt tccgccgcta cggtttccac ggcaccagta
    17881 tgcgttacgt tgcccctgaa gccgcacgca tcttgggcaa acctttggaa gacatccgca
    17941 tgattattgc ccacttaggc aacggcgcat ccattaccgc catcaaaaac ggcaaatccg
    18001 tcgataccag tatgggtttc acgccgatcg aaggtttggt aatgggtaca cgttgcggcg
    18061 acatcgatcc gggcgtatac agctatctga cttcccacgc cgggatggat gttgcccaag
    18121 tggatgaaat gctgaacaaa aaatcaggtt tgctcggtat ttccgaactt tccaacgact
    18181 gccgcaccct cgaaatcgcc gccgacgaag gccacgaagg cgcgcgcctc gccctcgaag
    18241 tcatgaccta ccgcctcgcc aaatacatcg cttcgatggc tgtggcctgc ggcggcgttg
    18301 acgcactcgt gttcaccggc ggtattggcg aaaactcacg taatatccga gccaaaaccg
    18361 tttcctatct tgatttcttg ggtctgcaca tcgacactaa agccaatatg gacaaacgct
    18421 acggcaattc gggtattatc agcccgaccg attcttctcc ggctgttttg gttgtcccga
    18481 ccaatgaaga actgatgatt gcctgcgaca ctgccgaact tgccggcatc ttgtagccaa
    18541 aaaagggacg agtccgcaca atgccgtctg aaattttcag acggcatttt gtttggattt
    18601 tgggcgtaca atacggaaaa catgacgata aggacacaaa ccatggcaca gtttttcgct
    18661 attcatcccg acaatcccca agaacgcctc atcaagcagg cggttgaaat cgtcaatcaa
    18721 ggcggcgtgg tggtttatcc gaccgattcg tgttacgcct tgggttgcaa actcggcgat
    18781 aaggcggcga tggaacgcat actctccatc cgcaaaatcg atttgaaaca ccacctgacc
    18841 ctgatgtgcg ccgatttgag cgagttgggc acgtacgcca aaattgacaa cgtacagttt
    18901 cgtcagctta aagccgccac gcccgggcct tataccttta tcttgcaggc gacgaaggaa
    18961 gtgccggcgc gcacgctgca cccgaaacgc aaaaccatcg ggctgcgtat tcccgataat
    19021 gccattgcac aagccctgct gggggaattg ggcgagccgc ttttaagctg caccctgatg
    19081 ctgcccgaag acggcgaacc attgaccgat ccttatgaaa tccgcgagcg tttggaacac
    19141 gccgtcgatt tggtgattga cggcggctgg tgcggaaccg agccgaccac cgtcgtcgat
    19201 atgaccgacg gcacggaact ggtgcgccgg ggttgtggcg atacggcggt gttcggtttg
    19261 tagggaaacc gatgccgtct gaagcatcgg ctgttcagac ggcattgcgc gccttgccgg
    19321 cggcagtccg aaatgccggc gcgtatcgcg ctcggtcgga atatccgttt gaaacggcat
    19381 tttgatgcat tactgcaccg caatcggaat tctcggttcg tagagcaggt cgtaggtcgg
    19441 cttgttgagc aggtcttgga gcgtgaaacc gtccagatac gtgaaaaacg acttcatcgc
    19501 gccgccgagt atgcccgtca gccggcagga cggtgtaatc aggcattcgt tgttctcgcc
    19561 catgcactcg accagctgca tcggttcgag gtggcggaca accgagccga tgttgatgcg
    19621 gtcgggcggt gcggcaagcc gcagaccgcc gccttttccg cgcacactgt ggaggaagcc
    19681 gcctttgacc agcgcggtaa cgaccttcat cagatggctt ttggaaatgc cgtaggttac
    19741 ggcgatggta ctgatgttga ccagcgcatc gtcgttgatg gcagtgtaga taaggacgcg
    19801 cagcccgtag tccgtatgtt gtgtcaaata catgattttc tcggtatgga ttgttattct
    19861 tatcggtacg gtttaaggtt cacggacaat accttaatgg ttgaaaccct gtccgtcggg
    19921 gcggtagaat gcagcctgtc tgcggcggta tgccgtctga aacatccgcg ctaccgtttg
    19981 ataatttgtt attgtaactc aaaatcatga aaccgttgaa acgacatccc gcccttatcg
    20041 ggctttcgcg tgaccaccac cattcgcttt ccctgtgcgt gcgtctgttg cggacgccgg
    20101 aagaaaggca tcgggacgaa ctcgaaccgc attttgccga attggaaacc cattttcgcg
    20161 aagaagaagc caagtttgcc ccaatttggc agaatgtcgc ccccgaattg aaacaacgtt
    20221 tcgagaaaga ccacgcccga ctgcggcaga tgatggcaag ccccgaatac ggtaacgcgg
    20281 cgtggaatac cgcttttgcc acaaccctgc gcgaccacgc gcgctttgaa gaacgcgagc
    20341 tgtttcccgc cgccgaaccg tttttgccgg catgattccg ttttgcggca aatatattaa
    20401 tgataaacaa ggaacacaca tgaaatttac caagcacccc gtctgggcaa tggcgttccg
    20461 cccattttat tcgctggcgg ctctgtacgg cgcattgtcc gtattgctgt ggggtttcgg
    20521 ctacacggga acgcacgagc tgtccggttt ctattggcac gcgcatgaga tgatttgggg
    20581 ttatgccgga ctggtcgtca tcgccttcct gctgaccgcc gtcgccactt ggacggggca
    20641 gccgcccacg cggggcggcg ttctggtcgg cttgactatc ttttggctgg ctgcgcggat
    20701 tgccgccttt atcccgggtt ggggtgcgtc ggcaagcggc atactcggta cgctgttttt
    20761 ctggtacggc gcggtgtgca tggctttgcc cgttatccgt tcacagaatc aacgcaacta
    20821 tgttgccgtg ttcgcgctgt tcgtcttggg cggcacgcat gcggcgttcc acgtccagct
    20881 gcacaacggc aacctaggcg gactcttgag cggattgcag tcgggcttgg tgatggtgtc
    20941 gggttttatc ggtctgattg gtacgcggat tatttcgttt tttacgtcca aacggttgaa
    21001 tgtgccgcag attcccagtc cgaaatgggt ggcgcaggct tcgctgtggc tgcccatgct
    21061 gaccgccatg ctgatggcgc acggcgtgat gccttggctg tcggcggctt tcgcgtttgc
    21121 ggcaggtgtg atttttaccg tgcaggtgta ccgctggtgg tataaacccg tgttgaaaga
    21181 gccgatgctg tggattctgt ttgccggcta tctgtttacc ggattggggc tgattgcggt
    21241 cggcgcgtct tatttcaaac ccgctttcct caatctgggt gtgcatctga tcggggtcgg
    21301 cggtatcggc gtgctgactt tgggcatgat ggcgcgtacc gcgctcggtc atacgggcaa
    21361 tccgatttat ccgccgccca aagccgttcc cgttgcgttt tggctgatga tggcggcaac
    21421 cgccgtccgt atggttgccg tattttcttc cggcactgcc tacacgcaca gcatacgcac
    21481 ctcttcggtt ttgtttgcac tcgcgctttt ggtgtatgcg tggaagtata ttccttggct
    21541 gattcgtccg cgttcggacg gcaggcccgg ttgagacaaa ccgccgcaga tttcgggtct
    21601 gggcggtttg cttttcagac ggcagggcgg tcagttgccg tccagccagc ggtcgcgtgt
    21661 ggttttggct tcttcaaaat agcggtacag ggcttcgcgg tcgtcggtgg tcaggatgtt
    21721 tgccaaaacg tccaactgtt tgcccaagcc ttgaaccagt tgcagcaggc tgtctttgtt
    21781 ggcaaggcag atgtccgccc acacggcggg atgaccggag gcgatgcggg tgaagtcccg
    21841 aaagcccgtg gcggcgaatt tcagatattc ctgtccgtcg gggtggtcga gaatctggtg
    21901 gacataggcg aaggcggtca ggtggggcat atgggagacg gcggcgaaaa ccgcgtcgtg
    21961 gcgttgcgcg tccatcgtat aaatttccgc cccgaccacg cgccacaggt tttctatcaa
    22021 ggcaatgccg tctgaatgtt cgccgccgtg tggcgtgatg atgagttttc tgtggcggaa
    22081 cagcccgaac tgcgcggctt gcgcaccgct tctgtccgaa ccggcaatcg ggtgggcggc
    22141 gatgcagcgg tgcaggcggt cgggcagaca gcggcggaag gcttcgatga ccgaagattt
    22201 ggtgctgccg acatcggaaa tccaagtgtg ttccggcaaa acggggcgca gcgcggtcaa
    22261 aatggcggga acggtggcga cgggcgtggc aatcagtacc aagtccgcac cgccgatgct
    22321 gtccgcgtcg atggcaacgg aagcccggtc aatcacgccg cgttccaatg cacgttcgag
    22381 gttgtcgcgg tcggtgtcga taccggtaac ggtgcggacg agtccctgcc ttttgaggtc
    22441 gagaacgaac gaaccgccga tcagccctac accgatgagg gcaatatggt tcaaaatggg
    22501 catttgtgta aacggttttc gcaaagtacc gtcatggtag cctatcggcg gaatatgccg
    22561 caaggtcggc aggaaaaagg agaagaaatg gacaaaatca gagttgccgc cgtgcagatg
    22621 gtgtcgggcg tgtcgccgga aaccaacgtc gctgccatga aacgcctggt cgcacgggcg
    22681 gcggagcagg gtgtggattg ggtgctgctg cccgaatatt gggtgctgat gggcgcaaac
    22741 gataccgaca aactcgcgct tgccgagcct ttgggcggcg gacgctttca gacggcattg
    22801 agcgaaacgg cgaaagaatg cggcgtggtg ctgttcggcg gaaccgtgcc gctgcaaagc
    22861 cccgaggcgg gcaaggtcat gaatacttta ttggtgtacg ggcgcgacgg caaacggacg
    22921 gggctgtacc acaaaatgca cctcttcggc ttttccggtt tgggcgaacg ctatgccgaa
    22981 gccgatacca tccgcgcggg cggggaagtg ccgcacttgt cggcagaggg cgtgccggtg
    23041 gcggcaggta tttgttacga tgtccgcttt cccgaatttt tccgccgcca gctgccgttt
    23101 gacgtattga tgctgcccgc tgcgtttacg cacacgacgg gcaaggcgca ttgggagctg
    23161 ctgctgcgcg cgcgtgccgt cgaaaaccaa tgttacgtcg tggcggcggc acagggcggt
    23221 ttgcacgaaa acggacggcg cacgttcgga cacagcatga ttgtcgatcc gtggggcgac
    23281 gtgttggacg tattgcccga aggcgaaggc gttgttacgg cagacatcga tgccaaccgc
    23341 ctgaacagcg tccgcaaccg cctgcccgcc ttgaaatacc gggttttgga tgccgtctga
    23401 aggttcagac ggcatcggtg ccggggaatc agcgggctgt aggatgggct tcagcctgcc
    23461 atttccatca atcccacatt tctaccgttt tcatcgaatc catcgaatcc gcccctttcg
    23521 acgacccagt cctacgcaac tgaaccgtca ttcccacgga agtgggaatc tagaatctcg
    23581 ggggtttcag tcatttccga tagattcccg ccgcgtcagg ggtctggatt cccgcctgcg
    23641 cgggaatgac ggatttcaag attgcgctgt tatcggacgg gtttcaagat tacggtgttg
    23701 tcggaacgca actgaaccgt cattcccacg aaagtgggaa tctagaatct tggggtttca
    23761 gtcatttccg atagattccc gccgcgtcgg aggtctggat tcccgcctgt gcgggaatga
    23821 cgggttttaa gattacggtg ttgtcggacg ggtttcgaga ttgc
//
LOCUS       DAXHTY010000021        23358 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXHTY010000021 DAXHTY010000000
VERSION     DAXHTY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23358
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.021"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(68..961)
                     /locus_tag="ACFNMK_000714"
     CDS             complement(68..961)
                     /locus_tag="ACFNMK_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6364029.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADFDTVELHNLQRQVAFNEGDVGKLKTEALADRLRHINHTVDVRAVNEKLDGCRL
                     TGLVQAADIVLDCCDNYATRQAVNRACVHTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAER"
     gene            1004..3706
                     /gene="ppc"
                     /locus_tag="ACFNMK_000715"
     CDS             1004..3706
                     /gene="ppc"
                     /locus_tag="ACFNMK_000715"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC6364030.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPDILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLSEEQKQAVLLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELDLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAVRNLETLVAATLEASLLPDQKDPEPALMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            3829..4818
                     /locus_tag="ACFNMK_000716"
     CDS             3829..4818
                     /locus_tag="ACFNMK_000716"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC6364031.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            4873..5202
                     /locus_tag="ACFNMK_000717"
     CDS             4873..5202
                     /locus_tag="ACFNMK_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364032.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            5213..5518
                     /locus_tag="ACFNMK_000718"
     CDS             5213..5518
                     /locus_tag="ACFNMK_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC6364033.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            5524..5703
                     /gene="ssrS"
                     /locus_tag="ACFNMK_000719"
     ncRNA           5524..5703
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFNMK_000719"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5881..6312
                     /gene="rplM"
                     /locus_tag="ACFNMK_000720"
     CDS             5881..6312
                     /gene="rplM"
                     /locus_tag="ACFNMK_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC6364034.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            6322..6714
                     /gene="rpsI"
                     /locus_tag="ACFNMK_000721"
     CDS             6322..6714
                     /gene="rpsI"
                     /locus_tag="ACFNMK_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC6364035.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(6969..7898)
                     /locus_tag="ACFNMK_000722"
     CDS             complement(6969..7898)
                     /locus_tag="ACFNMK_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6364036.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVSERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSGIS
                     FQPLFAYEMVGICAKDHPLAAKTVWEAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            8030..8779
                     /locus_tag="ACFNMK_000723"
     CDS             8030..8779
                     /locus_tag="ACFNMK_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC6364037.1"
                     /translation="MVLRRDFLTWCNETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            8902..9483
                     /gene="petA"
                     /locus_tag="ACFNMK_000724"
     CDS             8902..9483
                     /gene="petA"
                     /locus_tag="ACFNMK_000724"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC6364038.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            9502..10851
                     /locus_tag="ACFNMK_000725"
     CDS             9502..10851
                     /locus_tag="ACFNMK_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC6364039.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCSVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            10866..11654
                     /locus_tag="ACFNMK_000726"
     CDS             10866..11654
                     /locus_tag="ACFNMK_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC6364040.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            11993..13504
                     /gene="rmuC"
                     /locus_tag="ACFNMK_000727"
     CDS             11993..13504
                     /gene="rmuC"
                     /locus_tag="ACFNMK_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC6364041.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAHLAEKAA
                     RCDFVEQAHGKTVSELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQIEQERLASEEKLSLLGEARKSLSDQFQNLANTILE
                     EKSRRFTEQNREQLHQVLNPLNERIHGFGELVKQTYDKESRERLTLENELKRLQGLNA
                     QLHSEAKALTNALTGTQNKVQGNWGEMILETVLENSGLQKGREYVVQAASVRKEEDGG
                     TRRLQPDVLVNLPDNKQIVIDSKVSLTAYVRYTQAADADTAARELAAHVASIRAHMKG
                     LSLKDYTDLEGVNTLDFVFMFIPVEPAYLLALQNDAGLFQECFDKRIMLVGPSTLLAT
                     LRTVANIWRNEQQNQNALAIADEGGKLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQ
                     LAEGRGNLVGRAEKLRLLGVKANKQLARDLTERANETTALSESLEYAAEDEAV"
     gene            complement(13710..14342)
                     /locus_tag="ACFNMK_000728"
     CDS             complement(13710..14342)
                     /locus_tag="ACFNMK_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC6364042.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGSHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNHWLEEGETLKVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            14434..15258
                     /locus_tag="ACFNMK_000729"
     CDS             14434..15258
                     /locus_tag="ACFNMK_000729"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC6364043.1"
                     /translation="MIKKTIGGIIPIFMAVFIPASAGAADLMLAQEYKDQEIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     ASSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            15327..15890
                     /locus_tag="ACFNMK_000730"
     CDS             15327..15890
                     /locus_tag="ACFNMK_000730"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC6364044.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            15971..16408
                     /locus_tag="ACFNMK_000731"
     CDS             15971..16408
                     /locus_tag="ACFNMK_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC6364045.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            16477..16746
                     /locus_tag="ACFNMK_000732"
     CDS             16477..16746
                     /locus_tag="ACFNMK_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC6364046.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFQSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            16746..18521
                     /gene="ptsP"
                     /locus_tag="ACFNMK_000733"
     CDS             16746..18521
                     /gene="ptsP"
                     /locus_tag="ACFNMK_000733"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC6364047.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(18738..18947)
                     /locus_tag="ACFNMK_000734"
     CDS             complement(18738..18947)
                     /locus_tag="ACFNMK_000734"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364048.1"
                     /translation="MPSENLSDGIFLPKQPYFLSGLQNLSETTETTLPSFPQKRESRT
                     QHGKNLSEATTIFSSSFPRRRESRT"
     gene            complement(18965..19903)
                     /locus_tag="ACFNMK_000735"
     CDS             complement(18965..19903)
                     /locus_tag="ACFNMK_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6364049.1"
                     /translation="MLELNGLCKRFGNKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIWLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRNGGIPAVLVTHSPEEACTTADEIAVMHEGRILQYGTPET
                     LVKTPSCVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECCVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(19912..20787)
                     /locus_tag="ACFNMK_000736"
     CDS             complement(19912..20787)
                     /locus_tag="ACFNMK_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6364050.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLTRTWNKAGLLHGWRNEC
                     FDLTDGGGNILFSLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNTAAGGVSSGELPSETVCRESSEEAGLDKTLFPLIRPVSQLHSLRPVSWGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(20969..21045)
                     /locus_tag="ACFNMK_000737"
     tRNA            complement(20969..21045)
                     /locus_tag="ACFNMK_000737"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21009..21011),aa:Met,seq:cat)
     regulatory      21195..21294
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            21385..23286
                     /gene="thiC"
                     /locus_tag="ACFNMK_000738"
     CDS             21385..23286
                     /gene="thiC"
                     /locus_tag="ACFNMK_000738"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC6364051.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGGNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLEQHAGQSFGANIPTHPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIVDANDESQFSELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         5703 a         6330 c         6342 g         4983 t
ORIGIN      
        1 aatttttaaa tttttaaaac cgttccgccg catccgtttg gatgccgtct gaaacggtgg
       61 cagggtttta ccgttccgcg ccgcaaaccg ggcattcggg gttgcgcggc aggtcgaaat
      121 attgccagcc cccttccaag gcacggtaaa ccgccagcct gccgtgcgac ggttcgcccg
      181 catccagcag gattttcaga gcttccgccg cttgggtact gccgatgatg ccgaccagcg
      241 gcgagaacac gccgaagagg gaacagatgc cgtctgaagc cgatccgccg tcaaacaggc
      301 aggcgtaaca cggcgagtcg ggcaagtcgg gacggtacac ggcaagttgt ccttcaaagc
      361 gtaccgccgc ccctgaaacc agcggtgttt tcgtgtgcac gcaggcacgg ttgaccgcct
      421 gccgcgtggc gtagttgtcg cagcagtcta aaacgatgtc ggcggcttga accaaaccgg
      481 tcaggcggca gccgtcgagt ttttcgttga cggcgcggac atcgacggta tggttgatat
      541 ggcgcagacg gtctgccaag gcttcggttt tgagtttgcc gacgtcgccc tcgttaaatg
      601 cgacctgacg ttgcaggttg tgcagttcga ccgtgtcgaa atcggctatg gtcagcgtgc
      661 cgacacccga agcggcaagg tagggcagtg cggcggcacc caaaccgccg catccgacga
      721 ccaaaatatg tgcgtcggaa agcttttgct gcccttcgat gccgatttcg tccaagagga
      781 tgtggcggct gtaccgcagc aggagttcgt cgtcgttgtc gtgttcggtc gtggtcatga
      841 tgatgttcgg aaaaaacagt tgcgggcgat tgtaacgctg ccgtcgggcg gcgttcaact
      901 tcagacggca tttcgggaca cgggcggtta aagtgtgaac ggtttggcac ggatgcggca
      961 tttgaggtac atttacagta tttacggcaa tgagagaaaa atcatgcaac tgcatattct
     1021 gaacaatcca aaggacgcgg ctttggcggc ggacgcggaa tttctgaaac aatccctgtt
     1081 caacctcctg cacgaagaag cctcgccgtt ggttgtcgaa acggtcaaac tcttgtccac
     1141 ttccgacgac agcgcggcat tgattgaaaa agtattgccg caattggacg aacaacaaac
     1201 ccacgattta accttggcct gcggcctgtt cgcacagatt ttgaacatcg ccgaagacgt
     1261 gcaccacgaa cgccgccgcc aaatccacga agaagccgga cgcggcggcg cggaaggcag
     1321 cctgacggaa accgtccgca ggctcaaagc ggggaaagcc gacggcaaat cggtgcagcg
     1381 gcagttggac aatacgtccg ttaccgccgt tttgaccgcg cacccgaccg aagtgcaacg
     1441 ccaaaccgtt ttgaatttca accgccgcat ccgcgcgctg ctgccgcaac gcgaacgctg
     1501 caccaatgcc gacgcgctgg cacggctgcg ccgcgaaatc gacactatcc tgctgggctt
     1561 gtggcagacc agcgaaacgc gccgccacaa actcagcgtc aacgacgaaa tcaacaacgg
     1621 cgtgtccatc ttcccgatga gctttttcga agccctaccc aaactctacc gcaagatgga
     1681 acacgacttt cagacggcct atcccgatgt ccgcgttccg gacatcctca aaatcggcgg
     1741 ctggatcggc ggcgaccgcg acggcaatcc gtttgtttct gccgaaaccc tgcgctttgc
     1801 cttccgccgc cacgccgatg ccgtgttccg tttctatcgc ggcgaactcg acaaacttta
     1861 ccgcgaactg ccgctctcca tccgccgcgt caaagtcaac gacgacgtaa tggcactggc
     1921 agccctctcg cctgacgaag aaatcgcccg cacggaagaa ccctaccgcc gcgccatcgc
     1981 ctacattatg gcgcgcgcta tgggcaaagc gcgcgcgctc ggtttgggta tgggctgcaa
     2041 attcggcttt ctcgagcctt atgcttcggc acaagaattt ctcgatgatt tgaaaaaact
     2101 gcaacgttcc cttatcgaca acggcagccg cctgcttgcc gaaggccgtc tggcagacct
     2161 catccgctcc gtatccgtgt tcggcttcca tatgatgccg cttgatttgc gccaacacgc
     2221 gggcaaacac gccgatgtgg ttgccgagct tttccaacac gccggcctgg aagactacaa
     2281 cagcctctcc gaagagcaga aacaagccgt cctgttgcgc gaattgagtc atcaacgtcc
     2341 gctgtacagc ccgttcatca catacagcga ccatacccgc cacgaactgg caattttcaa
     2401 cgaagcgcgc aaaatcaaag acgaatttgg cgaagatgcc gtaacacaaa gcattatttc
     2461 caactgcgaa caacccagcg acctgctcgc cttggcattg ctgctgaaag aaagcggcct
     2521 gttggcggtg gaaaacggca aaccgcacag ccgcatcaat atcgtgccgc tgtttgaaac
     2581 catcgaagca ctcgaaaacg cctgtccggt catggaaacc atgttccgcc tcgattggta
     2641 cgatgccttg ctcgaaagcc gcggcaatat ccaagaaatc atgctgggct actccgactc
     2701 caacaaggac ggcggctacg ttaccagctc atggtgcctc taccaggcgg aattggactt
     2761 ggtcgaactc ttcaaaaaat acgatgtccg catgcgcctg ttccacggac gcggcggcag
     2821 cgtcggacgc ggcggcggcc cgtcttacca agccattctc gcccaaccgg caggcagcgt
     2881 ggccggacaa atccgcatca ccgagcaagg cgaagtcatt accgccaaat acgccgaccc
     2941 gggcaatgcc gtgcgcaatc tcgaaaccct tgttgccgca acactcgaag ccagcctgct
     3001 gcccgatcaa aaagaccccg aacctgcgct catgcaggca ttgtcggacg tatcgttcaa
     3061 atactaccgc gaactgatta cccaccctga cttcatcgac tacttcctgc aaaccagccc
     3121 gattcaggaa attgccaccc tcaacctcgg cagccgtcct gccagccgca aaaccttggc
     3181 gcggattcag gacttgcgcg ctattccgtg ggtattctca tggatgcaaa accgcctcat
     3241 gctgcctgct tggtacggtt tcggcagcgc ggtggaaacc ttgtgcgaag acaaacccga
     3301 aacgctcgcc gccctgcgcg aacacgccca aagcaacccg ttcttccaag ccatgctttc
     3361 caatatggag caagtgatgg cgaaaaccga catcaccctc gcggaaaact atgccggctt
     3421 gagcgaatcg ccggaaaaag ccgccgtcat cttcggcatg attaaggaag aataccaacg
     3481 cagccgcaaa gccctgctcg acctgctgca aaccgaagaa cttttacgcg acaaccgcag
     3541 cctcgcccgt tcgcttgcct tgaggattcc atatttgaac gcgctcaacg gcctgcaggt
     3601 cgccatgctc aaacgcctgc gtaaagaacc cgacaatccg cacgcgctgc tgatggttca
     3661 cctgaccatc aacggcgtgg cgcaaggttt gcgcaataca ggctgattgt gccgcatcgg
     3721 ggcaaaatgc cgtctgaaca cctttcagac ggcatttccc tgaccgcact tgcagagaaa
     3781 caccgattgt tttaaagtga acggcagtga tatgttgaaa gacgaccaat gaaaattacc
     3841 gttatcggcg caggttcgtg gggtacggcg ctcgccctgc acttttccca acacggcaac
     3901 cgcgtatccc tgtggacgcg caacgcagac caagtccgcc aaatgcagga agcgcgtgaa
     3961 aacaaacgcg gactgcccgg cttttccttt cccgaaacct tggaagtgtg tgcggatttg
     4021 gcagacgcgc tcaaagacag cggacttgtc cttatcgtaa cctccgttgc cggattgaga
     4081 agcagcgcag agctgctcaa acagtacggc gcgggacacc tccccgtcct cgccgcctgc
     4141 aaaggattcg agcaggatac agggctgctg acctttcaag tattgaaaga agtattgccc
     4201 gacaataaga aaatcggcgt actctccggc ccgagttttg cacaggaact cgccaaacaa
     4261 ctgccctgtg ccgtcgtcct cgcctccgaa aaccaagagt ggattgaaga actcgtaccg
     4321 cagctcaaca cgactgtcat gaggctttac ggcagtaccg acgttatcgg cgtggcggtt
     4381 ggcggcgcgg taaaaaatgt tatggcgatt gccaccggat tgtccgatgg cctagagtac
     4441 gggcttaacg cccgtgccgc actggttacg cgcggattag ctgaaattac ccgccttgcc
     4501 tccgcaatgg gcgcacagcc caaaaccatg atggggctgg caggcatcgg cgacctcatc
     4561 ttgacctgca ctggcgcact ctcacgcaac cgccgcgtcg gcttgggttt ggcagaaggc
     4621 aaggaactgc atcaggtgct ggtcgaaatc ggacacgttt ccgaaggggt cagcacgata
     4681 gaagaagtct tcaatactgc ctgtaagtac caaatcgata tgccgattac ccaaaccctg
     4741 ctgcaactca tccgtaagga aatgacccct cagcaggtcg tcgaaagact gatggaacgc
     4801 agcgcgcgtt tcgaataaac aacagacaga tgccgtctga agccttcaga cggcatacgg
     4861 acaggtaagg ttatgaaaca aaatatcgaa aaactcgaaa gcagcgttta tacgctggta
     4921 caaaaattcg aaaccctcgt cagcgaaaac cgccgcctca aagaaaccgt cgccgaactc
     4981 aaacgggcgc acgagcggca aaaactcgaa cacgaaaccg ccgtcgacga actcagcgaa
     5041 gccctgctcg tccaagtcgg caaactcaaa gaagacctgc aaaacaaaat cgacagcctg
     5101 acagaagaaa atacacgata ccgcagcctg ctcgaacaga gcagggaaaa aatcagcgca
     5161 ctggcagcgc gcctccccca atggcaggaa acgcagcaat aaggattaaa ggatgaacat
     5221 cgaacaagtc cacatcgaag tcatgcacgc ccggctgacc gtcaacacgc cggcagaaga
     5281 aaaagacaca ctgttgcagg cagtcggaat gctcaacggc aaagccgaag ccatccgcga
     5341 aggcggacgc gtcgcggaca gcgaaaaaat cgtcattatg gccgcgctca acgtcgtcca
     5401 cgacctgttg aaaacctctc tgaacggcgg cgatttggca atcggcgatt ttgcgcgtaa
     5461 aatagccgat atggacaacg cctgccaaaa agcactatcc cgcttggcgc aggaataacc
     5521 cctttttccc tgcggtgtcc gcgaaggcat atattccttt gaaccaataa gttcgcttaa
     5581 ggttgccggg agcagtagtg ggcgcgagcg tccttttgcg gacgcacccg aaactacccg
     5641 aggcagccgc cttgtcagca aggttcaagc ggattcggcc gaaacggctc tcgcggggat
     5701 tcccattcaa aaccgcatcg caacgatgcg gtttttcaca ttggccgccg ccccgcccga
     5761 aaaagcagta tgcagtctgc cgaaatccgg aaaatccttc cgtaacgcgg taatgcgatt
     5821 gacaaaatcg aatcctgtct ttaaaattta caactttcta tatctatctg gatttccact
     5881 atgaaaacct tttcagcgaa accccacgag gtgaagcgcg aatggttcgt catcgatgcc
     5941 caagacaaag tcttgggtcg cgttgcggcc gaagtcgcca gccgtctgcg tggcaaacac
     6001 aaacctgaat acacccccca cgtcgatacc ggcgattaca tcattgttat caatgcggac
     6061 aaactgcgtg taaccggtgc caaattcgaa gataaaaaat acttccgcca ttccggtttc
     6121 ccaggcggta tctacgaacg cactttccgc gaaatgcaag agcaattccc gggccgcgct
     6181 ttggaacaag ctgtaaaagg tatgctgccc aaaggtcctt tgggttacgc catgattaaa
     6241 aaactgaaag tgtatgcggg tgcggaacac gcccatgctg cgcaacaacc caaagttttg
     6301 gaactgaaat aaggacgcga catgaacggt aaatactact acggcacagg ccgccgcaaa
     6361 agttcagtgg ctcgtgtatt cctgattaaa ggtacaggtc aaatcatcgt aaacggccgt
     6421 cccgttgacg aattcttcgc acgcgaaacc agccgaatgg ttgttcgcca acccttggtt
     6481 ctgactgaaa acgccgaatc tttcgacatc aaagtcaatg ttgttggcgg cggcgaaacc
     6541 ggccagtccg gcgcaatccg ccacggcatt acccgtgccc tgatcgactt cgatgccgcg
     6601 ttgaaacccg ccttgtctca agctggtttt gttacccgcg atgcccgcga agtcgaacgt
     6661 aaaaaaccgg gtctgcgcaa agcacgccgt gcaaaacaat tctccaaacg ttaatgttgg
     6721 aaattcaaaa aaccctgctt atcgcagggt tttttatttg tagtagacgg tttcccattg
     6781 gcaatctaaa gattacagat tgggcaaaaa tcaaaaacag tatagtggat taacaaaaat
     6841 caggacaagg cgacgaagcc gcagacagta caaatagtac ggcaaggcga ggcaacgccg
     6901 tactggtttt tgttaatcca ctatacatga aaaaatagaa aaactgccgg gtatggttga
     6961 ataaggggtc agaccggttc cagttcgctc agtccgggca aatctgcaaa accgcgttcg
     7021 cgtatgattt ggcaaaaatt gttcagatag ctcttgtccg tatcttcggt acggatggcg
     7081 gcatacagtt tgctttgcag tccgtcggca gtaatttggc ggtggacgac atagcctttt
     7141 tcaaggtagg gcatgactgt ccaataggga agggcggcaa tgccacgtct gctggcaacc
     7201 agttggataa tggcgatggt cagttcgctg tgtcggcgcg gcgggttgat gtttttcgga
     7261 atcaggattt ttttgggcaa atccagcatc tcgtcgggaa cgggataagt aatcagggtt
     7321 tccccgataa agtcttcggc ttcccagaca gttttggcgg caagcgggtg atctttggcg
     7381 cagatgccga ccatttcgta ggcaaacagt ggttggaagc tgatgccgct ttgtttttcc
     7441 gcttcggaaa caatggcaag gtcggcgcgg tgttgcagca gcagcccgac gggatccgct
     7501 tggaatcccg atacgatatc cagttcgact tggggccaca tcgggcggaa ttcgcccatg
     7561 gcgggcatca gccagtcgaa acaggtatgg cattcgacgg caatccgcag ctctcccgcc
     7621 tccccttccg tgattcgcgc caaatctcgt tctgcaacag caacttgagg tatcagttcg
     7681 tgggcgaggc gcagcagcct ttcgctcacc ggggtaaagc gcaagggcgt ggatttgcgt
     7741 tcgaacagcg gcgtgccgta gtggttttcg agcatacgga tctggtggga aagggcggat
     7801 tgggtaagga aaacccgttt ggcggcaagg gagacgctgc cggtttcttc aagtgccagc
     7861 agggttttga ggtggcgcaa ttcgataatg gaatccatgg gggcttcaga cggcatattg
     7921 aaccggcgca tattaaaata attcatatgc gggtgcaaat cggaaaaatg tgaattccgg
     7981 acattttgcg attagaatgc ccgcttgttt aaagcgatta ggaaaaaaga tggtattgcg
     8041 cagggatttt ctgacttggt gtaatgaaac gttgcagacg gcatcgttta aagattacgc
     8101 ccccaatggt ttgcaggttg aagggaggga atatatcggg aaaatcgtta cgtcggtaac
     8161 ggcaagcagg gcagcgattg attttgctgt ggagcagaag gcagatttgc ttttggtaca
     8221 tcacggtatg ttctggaaaa acgagttgcc gaccgttact ggttggaaaa aagaacggat
     8281 tgccgcactg ttacggcacg acatcaatat ggcaggctac catctgcccc tggatgcaca
     8341 tcccacactg ggcaacaatg cccaactcgc cgacagattg ggttttgcga cagaaaaacg
     8401 gttcggcgaa caaaacctgc tcaactcggg cagcctgaaa caagccaaga cactcggcgc
     8461 attggcggcg catattgaaa cagttttgca acgtaagcct gtcgtcatcg gcaaacccga
     8521 acgcgaaatc cgacgggttg catggtgcag cggcggggcg caggggtttt ttcagacggc
     8581 aatagacgaa ggtgtcgatc tgtatttgac gggggaaatc tctgaagccc aataccacct
     8641 tgccaatgaa acgggtacgg ctttcatttc ggcagggcat cacgcgacgg aacgttacgg
     8701 cgtacgcgcg ctggcagaat cggcggcaga ggttttcggg ttggaagtgt gccattttga
     8761 cgaaaacaat ccggcttgag tcttgagaaa tatcataaaa ctttacctta ttttaaataa
     8821 tgctttgagt atcactgcaa actgggtaaa attgcaacgt ttaatggctc ggtattccca
     8881 aaatattagg acgactgaat aatggataat caagaaatca acaacggccg ccgccgtttc
     8941 ctgacactcg cgacctgcgg cgcgggcgga gtggcagcat tgggtgtggc aacgccgttt
     9001 gtggccagtt ttttcccttc ggaaaaagcc aaggccgccg gtgctgccgt cgaggtggat
     9061 gtcagtaaaa tcgaagcggg tcagctgctg accgccgagt ggcaaggcaa accgatttgg
     9121 gtgctcaacc gtacagatca gcagcttaaa gacctgaaag gcctgaacgg cgaacttacc
     9181 gatcccaatt ccgatgcgga acagcagccg gagtatgcta aaaacgagac ccgttcgatt
     9241 aagccgaaca tccttgtcgc catcggtatc tgcacccatt tgggctgctc gcccaccttc
     9301 cgtcccgaca ttgcccctgc cgatttgggt gcagactgga aaggcggctt cttctgtccc
     9361 tgccacggtt cgaaattcga cttggccggc cgcgtatata aaggtgttcc tgccccgacc
     9421 aacctggttg tcccaccata taaatacttg agcgacacaa ctatcttggt gggcgaagac
     9481 taagaataag gaacgataat tatggcaaac caaaccaata gcaaagcaaa agcattgtta
     9541 ggctggatgg acgctcgttt ccccctgtct aaaatgtgga atgagcattt ggctcaatac
     9601 tacgcaccga aaaactttaa tttttggtac tacttcggct ctttagcctt gcttgtcctc
     9661 gtgattcaaa tcgtcagcgg cattttcctg accatgaact acaaacccga cggcaacctg
     9721 aacgcctacc atctgcctgc cgcctttacc gcagtagaat acattatgcg cgacgtgtcc
     9781 ggcggttgga ttatccgcta tatgcactcc accggcgcat cgttcttctt cattgttgtt
     9841 tatctgcaca tgttccgtgg tttgatttac ggttcgtaca aaaaaccccg tgagttggtg
     9901 tggattttcg gctccctgat tttcttggca ttgatggcgg aagcctttat gggctacctg
     9961 ttgccttggg gtcaaatgtc cttctggggt gcgcaggtaa ttattaactt gttctccgcc
    10021 atccctgtta tcggtcctga tttgtccact tggatccgcg gcgactttaa cgtttccgac
    10081 gttaccctga accgcttctt tgccctgcac gttatcgcgg tacctctggt attgctcggc
    10141 ttggttgtgg cgcacatcat cgccctgcac gaagtgggtt ctaacaaccc tgacggcgtt
    10201 gaaatcaaga aaaacaaaga cgaaaacggt atcccgcgcg acggtatccc attccatcct
    10261 tactacaccg ttaaggacat cttgggtgtc gttgtattcc tgattgtctt ctgttccgtg
    10321 ttgttctttg caccagaagg cggcggctac ttcctcgaag cgccgaactt cgatgctgcg
    10381 aatgcgctga aaacacctcc gcacattgca ccggtatggt acttcactcc gttctacgcc
    10441 attttgcgtg cgattccctc gttcttggga acacaggtat ggggtgtaat cggtatgggt
    10501 gcggcagttg tactgattgc cctgttgcct tggctggata ggggcgaggt gaaatccgtc
    10561 cgttaccgcg gcccgatttt caaaaccgca ttggtcttgt tcatcatttc ctttatcggt
    10621 ttgggtattt tgggcgcgat ggtggcaacc gatacgcgta ctttggtcgc gcgcatcctg
    10681 tccttcgtct atttcgcatt cttcctgggt atgccgttct ataccaagct ggataaaaac
    10741 aaaccggttc ccgaacgcgt aaccatgagc accgccaaac aaaaaatcat gttctttgtt
    10801 tacgtcggca ttaccgccgt gggtgcttac ctgtttgcaa ccaacatctg atgagggcag
    10861 tcaaaatgaa acaagctatg aaaaattggt ttgctgcctt gctgttggca gtacccatgg
    10921 gtgcggcatt tgcttcgggc ggtcatgcac actatgagaa agtcgatatc gatttgcgcg
    10981 accaagtcag cctgcagcgc ggcgcacaaa tctttaccaa ctattgcctg tcctgccact
    11041 cggcaagcgg tatgcgtttc aaccgtttga aagacatcgg tttgactgac gaagaaatca
    11101 agaaaaacct gatgtttacc accgataatg tcggcgatgt catgcattcg gcgatgaacc
    11161 cgaaagatgc ggcaaaatgg tttggtgctg ctccgcccga tttgacattg attgcgcgtt
    11221 ccaaaggtgc agactacctt tacgcctata tgcgcggctt ctataaagat ccgacccgtc
    11281 cgagcggctg gaacaatacc gtatttgata aagtcggtat gcctcacccg ctgtgggaac
    11341 agcaaggcgt tcaagccgtt gagttggatg ccaaaggtca gccggttatg gtaaaagacg
    11401 aacacggcga gatgaagcct aagctgtatt gggaatctac cggtttgcac agccgccgtc
    11461 tgcctaacgg caaagtgatc caaaaagagt acgacgcata tgtacgcgat ttggtcaatt
    11521 accttgtgta catgggcgaa cctgcacaac tgcaacgcaa acgtataggc tatgtcgtga
    11581 tgattttcct atttgcggtt atgctgcctt tggcgtattt cctgaaaaaa gaatattgga
    11641 aagacgtaca ctaagcgttt ggaacaaaag ggcaaatcct ttagggtttg cccttttttc
    11701 attttgcctg ccgtttgaaa agcctgaatc cgtatgccgt ctgaacatag ctgcaacatt
    11761 tcagacggca tcattctaaa aatgtcagac atcgggaact taatcagggt ttgcggcgga
    11821 tggatattga attaggaaaa ggcctgaaac cgcaagcagc atggtaaggc gatgtcgaac
    11881 aggtttaaag ccaatccgct atattttttg gagctatgtc gaacaaacag gtagaatcgg
    11941 aataagcggc ggttttgtcc ggtgtgaaaa tgtttgaata ttaggatgat tgatggaact
    12001 gatgactgtt ttgctgcctt tggcggcgtt ggtgtcgggc gtgttgttta catggttgct
    12061 gatgaagggc aggtttcagg gcgagtttgc cggtttgaac gcgcacctgg cggaaaaggc
    12121 ggcaagatgt gattttgtcg aacaggcgca cggcaaaacc gtgtcggaat tggcggtgtt
    12181 ggacgggaaa tatcggcatt tgcaggacga aaattatgct ttgggcaacc gtttttccgc
    12241 agccgaaaag cagattgccc atttgcagga aaaagaggcg gagtcggcgc ggctgaagca
    12301 gtcgtatatc gagttgcagg aaaaggcaca gggtttggcg gttgaaaacg aacgtttggc
    12361 aacgcaaatc gaacaggaac gccttgcttc tgaagagaag ctgtccttgc tgggcgaggc
    12421 gcgcaaaagt ttgagcgatc agtttcaaaa tcttgccaac acgattttgg aagaaaaaag
    12481 ccgccgtttt accgagcaga accgcgagca gctccatcag gttttgaacc cgttgaacga
    12541 acgcatccac ggtttcggcg agttggtcaa gcaaacctat gataaagaat cgcgcgagcg
    12601 gctgacgttg gaaaacgaat tgaaacggct tcaaggattg aatgcgcagc tgcacagcga
    12661 ggcaaaggca ctgaccaacg cgctgaccgg tacgcagaac aaggttcagg gcaattgggg
    12721 cgaaatgatt ctggaaacgg ttttggaaaa ttccggcctc cagaaagggc gcgaatatgt
    12781 ggttcaggcg gcatccgtcc gaaaagagga agacggcggc acgcgccgcc tccagcccga
    12841 cgttttggtc aacctgcccg acaacaagca gattgtgatt gactccaagg tctcgctgac
    12901 agcttatgtg cgctacacgc aggcggcgga tgcggatacg gcggcacgcg aactggcggc
    12961 acacgttgcc agcatacgcg cgcatatgaa aggcttgtcg ctgaaggatt acaccgattt
    13021 ggaaggtgtg aacacattgg atttcgtctt tatgtttatc cctgtcgaac cggcctacct
    13081 gttggcgttg cagaatgacg cgggcttgtt ccaagagtgt ttcgacaaac ggattatgct
    13141 ggtcggcccc agtacgctgc tggcgacttt gcggacggtg gcgaatattt ggcgcaacga
    13201 gcagcaaaac caaaacgcgc tggcgattgc ggacgaaggc ggcaagctgt acgacaagtt
    13261 tgtcggcttt gtacagacgc tcgaaagcgt cggcaaaggc atcgatcagg cgcaaaacag
    13321 ctttcagacg gcattcaagc aacttgccga agggcgcggg aatctggtcg gccgcgccga
    13381 gaaactgcgt ctgttgggtg tgaaggcaaa caagcagctt gcacgcgatt tgaccgaacg
    13441 tgccaatgaa acaacggcgt tgtcggaatc tttggaatat gcggcagaag atgaagcagt
    13501 ctgacttgtg cggaaaagct tgtttcagca ggggcgggaa tgccgaaagc gcggcgtaac
    13561 tgtaccggtt ctgatttggg gttggtttga agtacttacc aaagccttgt ccggcgaggt
    13621 acaccggcaa ggcggcggtt gtctgagcct tttgcgtttc agacggcatc agtgcagatg
    13681 acgccgtctg aagaccgtag tgaagtcggt tagaaaaacg gattgtgccg tttttcgtgt
    13741 ccgatggaag tcatacgccc gtgtccggcg acaacttgca cggtttcggg gagggcgaat
    13801 aatttgttgc ggatattatt gattaagtcg gcgtggttgc cgcgcggaaa atcggttctg
    13861 cctatggttt cgtaaaacag cacgtcgccc gcaatcagca gctccgcttc ggcacaatag
    13921 aaaaccacgt gtcccggcgt gtgccccgga atatgcagca cttggaaggc gtagcgtccg
    13981 actttgagcg tttcgccttc ttcgagccaa tggttcggcg caaaggcggg cgagacgggg
    14041 aatccgtatt gcgcggtggt ttgcggcagc gattggagca ggaattcatc ttcgcggtgc
    14101 gaaccgagga cagggacttt atgcgttttc aacatttcga ccacgccgcc cgcgtgatcg
    14161 agatggccgt gcgtcagcca gattgccgtg agcgtaagtt tgcggtttgc caacgcttgc
    14221 agcaggaacg gcacatcgcc gccgacatcg gtcaggacgg cttcgccact ttcgtcgtcc
    14281 caaatcaggg tgcagttttg gcggaagggg gtaacgggga agatttcgta acgtagggtc
    14341 atcggcgtgt tcctaaacgg tttttcagac ggcatcgggt ttgccgtttg ttttatggcg
    14401 gtttgccgcc cgttttgata ttgggggaat cagatgatta agaagacaat cggcggcatc
    14461 ataccgattt ttatggcggt tttcatccct gcatcggcag gcgcggcgga tttgatgttg
    14521 gcgcaggaat acaaagacca agagattgca ggctgggcaa tgagcgagaa actcgacggc
    14581 gtgcgcgcct attgggacgg aaagcacctg ataagccgtc agggctacgc gtttacgccc
    14641 cccaaaggct ttaccgcgca gtttccgcct tatcctttgg acggcgaatt gtatagcgga
    14701 cgcggtcagt tcgagcagat ttccgctacc gtgcgttctg cttcttcaga ctggcgcggt
    14761 atccgcctgc acgttttcga tgtacccaag gcgcagggca atctctacca acgtttggca
    14821 gtcgcaacgc agtggctgaa aacgcatccg aacgcgccga ttaccatcat cccacaaatc
    14881 aaagtgcgcg accggcagca cgcgatggac tttttaaaac aaatcgaagc gcagggcggc
    14941 gaaggcgtga tgctgcgtca gcccgaatcc cgttacagcg gcggcaggag cagccaatta
    15001 ttgaagctga aaagccaata cgacgacgaa tgcacggtaa cgcggcacta tgagggcaaa
    15061 gggcgaaacg ccggacggct gggcgcggtc ggctgcaaaa accgacacgg cgaattccgc
    15121 atcggcagcg gtttcaaaga caaagaccgc gacaacccgc ccaaaatcgg cacactgatt
    15181 acctaccgct accgtggttt tacgcggaaa ggcacgccga aatttgccac atttgtgcgc
    15241 gtgcgtaccg accgctgagc aggacggttc agacggcagc cgatactttg gtttataatt
    15301 ttccttttac cgaccgattc cgacatatga ccgatttaga aaccaaacgc cttgaaacac
    15361 aggcgatgct tgaaaacgcc gatcttttgt tcgaccaagg ccaatgccgt gccgcactgc
    15421 aaaaagtggc ggacgagatt acgcgcgatt tgggcggcaa atatccgctg ctgctgcccg
    15481 tgatgggcgg cgcggtggtg tttacggggc agttgttgcc gctgttgcgt tttcccttag
    15541 attttgatta tgttcacgtt tcccgttacg gcgacaagct ggagggcggc gcgttcaact
    15601 ggaagcgtat gcccgatgcg gaacaaatcc ggggcaggca cgtcgtcgtg ctggacgata
    15661 ttttggacga agggcatacg atgtccgcca ttcaagccaa acttttggaa atgggtgcgg
    15721 caagctgccg tgcggcggtg ttcgccaaca agctgattga caaacccaag cctatccgag
    15781 ccgattatgt cggactggat gtgccgaacc gttatgtttt cggttacggc atggatgcgg
    15841 cgggctgctg gcgtaatttg ggcgagattt acgcattgga cggaaaataa ggacgcgatg
    15901 ccgtctgaag gcctttcaga cggcatcgcg cccatacgcc ggcaggataa tgaagaacag
    15961 gacgcataat atgatagggc ttttaatcat cacacacgaa accataggcg aagcctaccg
    16021 caagctggca catcattttt tcccgggcgg gctgcctgaa aacgtccgca tactcggcgt
    16081 gcagccgacg gaagaccaag acgacatcat caacaacgcc attgccgcgc ttcaggagtt
    16141 tcccgacaac gacggcgtgc tgattatgac cgatattttc ggcgcgaccc cctgcaatgc
    16201 agcccgccgc ctcgtgcgcg aaaacaaatc ggcgattttg accgggctga acgcgccgat
    16261 gatggttaag gccgtccaat attcgccggc ggcggaagac cttgccgcct ttaccgaatg
    16321 cgtcagggag gcggcggtaa aaggcatttt cgccatcacg tccgcgcccg aagatttggt
    16381 gtgccggcgc agcggcgatg ccgtctgaag aagcggcagg gcaggaaaac attttaaacg
    16441 gcgtctgctg ccgatataca taacacggga atcgaaatgc tcaaacaatc catcgaaatc
    16501 atcaacaaac tcggacttca cgcccgtgcc tccagcaaat tcacccaaac cgcgtctcag
    16561 tttcaaagcg aagtctgggt aacgaaaaac ggcagccgcg tcaacggcaa aagcatcatg
    16621 gggctgatga tgctcgccgc cgccaagggt acggtcatcg agctggagac ggacggcgcg
    16681 gacgaggcgg aagcgatgcg cgccctgacc gacttaatca acggctactt cggcgagggc
    16741 gaataatgag tatcgtgctg cacggcgtgg cggcgggcaa aggcattgcc gtcggttgcg
    16801 cccacctgat tgcgcgcggt acggaggaag tgccgcagta tgatgttgcg gaggcggaca
    16861 ccgatgccga agccgaacgt ttcgatgccg ccgtcaaagc cacgcgcaaa gagttggaac
    16921 agctccgcag cgcgattccc gaaaacgccc cgaccgagtt gggcgcgttc atctcgctgc
    16981 acctgatgct cttgaccgat gttaccttgt cgcgcgaacc cgtcgatatt ttaagggaac
    17041 aaaaaatcaa cgccgagtgg gcattgaagc agcagagcga caaactcgcc gcccaattcg
    17101 acaatatgga cgatgcctat ttgcgcgaac gcaagcagga tatgctgcaa gtcgtccgcc
    17161 gcatccacaa caacctgatc gggcagggca acgagttgga agttgccgac aacctgtttg
    17221 acgaaaccgt tctgattgca aacgaccttt cgcccgccga cacggttttg tttaaagagc
    17281 agcgcattgc cgccttcgtt accgatgccg gcggccccac cgggcatacg gcgattttgg
    17341 gcaggagctt ggacatcccg tccgtcgtcg ggctgcacaa cgcgcgcaaa ctgattaccg
    17401 agggcgaaac ggtcattgtg gacggtatca acggcgtgtt gattatcgcg ccggatgagt
    17461 cggtgttgaa cgaataccgc cgccgtgccc gcgaataccg cagccacaaa cgcgatttga
    17521 acaagctcaa aaaaaccgcc gccgccaccg ctgacggggt ctgcatcgag cttgtgggca
    17581 atatagaatc cgccgaagac gtgaaaccgc tgcacaacct cggcgcagac ggcatcgggc
    17641 tgttccgcag cgagtttctt tacctgaacc gcgatacgat gccgtctgaa gacgagcagt
    17701 acgaagtgta cagcgcgatt gtcaaaaaaa tgaaaggcaa aagcgtaacg atacggacag
    17761 tcgatttggg tgtggacaaa aacccgcgct ggttcgggaa aaacagcacg cccaacggca
    17821 gcctcaaccc cgcgctgggc atgaccggca tccgcctgtg ccttgccgaa ccggtcatgt
    17881 tccgcaccca gatgcgcgcc atcctccgtg cggcggtaca cggccccgtg cggatgatgt
    17941 ggccgatgat tacctccgta tccgaagtgc gccagtgcct catccacctc gacaccgcgc
    18001 aacgccagct tgccgaacgc ggcgatgcct tcggtaaagt cggcatcggc tgtatgattg
    18061 aaattccgtc tgccgcgctg accgtcggca gtattttgaa actggtcgat ttcatctccg
    18121 tcggtaccaa cgacctgatt caatacatct tgtccgtcga tcgcggcgac gacagcgtca
    18181 gccacctcta ccagcccggc catcccgccg tgctgaaaat gctgcaacac gtcatccgta
    18241 ccgccaaccg catggacaaa gacgtatccg tatgcggcga gatggcgggc gataccgcgt
    18301 ttacccgcgt tttattgggt atggggctgc gccgtttttc catgaacccc aacaacatcc
    18361 tgcccgtcaa aaacatcatt ctgcacagca atgtcggaca gctcgaaagc gatattgtga
    18421 aagtcatccg ctgcgaagac gaagagaagt cggaaaagct gatcaaacag atgaacagcg
    18481 tgtctgtcga ggaagaagcc gacttcaagg ggcggaaata aatacggcag gtaaaaaata
    18541 gaaatactta acaatgcccg caatctgaaa ttttgccatt cttgcaaaat agaaaaccga
    18601 aacagaaacc caaaatcggc cattccctca aaaacagaaa accaaaatca gaaacctaaa
    18661 atccgtcatt cccgcgcagg cgggaatcta ggtctgtcgg tgcggaaact tatcgggtaa
    18721 aacggtttct tgagatttta cgttctagat tcccgcctgc gcgggaatga cgatgaaaag
    18781 attgttgtcg cttcggataa atttttgccg tgttgggttc tagattcccg cttttgcggg
    18841 aatgacggca gggtggtttc tgttgtttcg gataaatttt gcagccctga taaaaaatat
    18901 ggctgctttg gtaaaaaaat gccgtctgaa aggttttcag acggcatttt gtttttaaga
    18961 agcatcagcg gaagcggacg atttccctgt cttcgatatg gatgcgtacc gtatcgtttc
    19021 ccgatatttc accggcgtgc ggcatatcga ggttcagcca caggatgccg tgttccggat
    19081 ggaggacgga caggctgaac gattcgggca aacaggtacg ggataatacg cagcactcca
    19141 tgccgtcttg gtcgaaacgc accgcatgtt gcggaatatg gcggtcatcg tcggtattgg
    19201 gcagccccat cagtcgggca acctgcacgc aggacggtgt tttgaccaat gtttcgggtg
    19261 tgccgtattg tagaatcctc ccttcatgca tcacggcaat ttcgtctgcc gtcgtacagg
    19321 cttcttcggg cgaatgagta accaagacgg cagggatgcc gccgtttcgg atacgttcgg
    19381 cggtcatgcg gcgcagccgg tcgcgcaaat gcgtgtccaa actggaaaac gattcgtcca
    19441 gcaacagcag ggaagggcgg acaaccaaag cgcgcgccag tgccaaccgt tgtttctcgc
    19501 ctccggaaag tttttcaggc ttacggtgcg cctcgttttc cagtccgact tcggcaagtg
    19561 ccgccatggc aaggcgttcg gcttcggctt tcggcatttt ttgcattttc aaaccgaatg
    19621 ccgcattttc cagcgcactc atatggggaa acagcgcgta atcttgaaac atcagagaaa
    19681 tacggcgttt ttcgggcggc atacgggtaa tgttttcccc gttcagccat atttccccac
    19741 cgtccggccg gacaatcccc gcaatcatat tcagcagggt ggattttccg catcccgacc
    19801 gccccaaaac ggcgagtatt ttgccgcgcc cgacagtcag gcagatgttg tcggcgacgg
    19861 ttttattgcc gaagcgtttg cagagtccgt tcagttcaag catggcgcat cctataaacg
    19921 tatgccgtcc agccactcgg acagcggatg ggcggcatca atcagaccgt aacggcaaaa
    19981 cgcgtccagc gtaaccagtt gcgcgtcgtg catcatgttt cccgacaaca tggtggcaac
    20041 caagctgccg atgtccattt tctcaaaaca cgcaacctca ccgtcctgat tttcaggtag
    20101 gaaggtttcg ggcaggacgg catcgaatac atacaggatt tcattgtgca caccccagct
    20161 gacggggcgc aggctgtgca gctgcgatac cgggcggatg agcggaaaca gcgttttatc
    20221 caaaccggct tcttcgctgc tttcgcgaca cacggtttca gacggcaatt caccgctgga
    20281 aacaccgccg gcggcagtat tgtcgagttt gtcgggatcg actgctttgt gcggactgcg
    20341 cctgcctatc cagaaatgcc atcggccgtc cgattcggtc agaccgttga gatggacggc
    20401 gcggctgagt agtccgaacg ggcggaaagc ggcgcgttca agtgagaaca agatattgcc
    20461 gccgccgtcg gtcaggtcga aacactcgtt gcgccatccg tgaagcagcc ccgccttgtt
    20521 ccatgtgcgg gtgaggtgct gcaagcggcc gcccatatct gaccagccgt ccgcattcag
    20581 aaaaatgccg tctgaagact ccgagcagcc tgcctcccag tcttttttga cgcgctccgc
    20641 ccattccggc gacaggttgc ccaaaggcag gccgttcaga tacagtgttt tccagcaact
    20701 ttctgcaccg taacttgctt ttgcccactc gaacagggca tcaaggtcgt gtttgctgac
    20761 ggattcggta aaacggacgg tcggcatagt cggctttctt gaagacaatg cggaagattg
    20821 tagccaaact gcctgaattt gcaaccgcaa acagacggcg ggctgccgtt ccgatattgc
    20881 ggcaggaagg aaaatccggc aaaaaacgga ggcggcagga aacaggcaaa acaaaacccc
    20941 tcggcattga accgaggggt ttgatatttg gttgcgggag caggattcga acctacgacc
    21001 ttcgggttat gagcccgacg agctaccatg ctgctccatc ccgcgtcaga agatgagact
    21061 atacggcaga ttttttatgt tgtcaaacat tacttccgcg aaaaatcata aaattttgcc
    21121 gtccggcggt ttatgcctga tttgacatat tattttcttt acaaaagttc atgttcgtga
    21181 tttaattttg gttaacattg aaacaggggt gctgcctgat gttcaggcgg ctgagaaata
    21241 ccctttacac ccgatcggga taatacctgc gtggggagtt ttcacggatt ctgtctttca
    21301 gacggcattg gttttcaaat gccgtctgaa aacgcaaaac gctcctgttt ctttattcta
    21361 aacgagaaaa caggagcatt ttttatgact acgccaaaaa aaaccgccaa aacttccggc
    21421 aacgaagcgc gcgagcttgc cgacttgagc gaagacatcg gcatccgctt caaatatccg
    21481 aactcggaac gcgtgtatct gcaaggcagc cgcgacgaca tccgcgtgcc tttgcgcgaa
    21541 atccgtcagg acgacaccta cacggcgcaa ggtacggaag ccaatccgcc gattcccgtc
    21601 tatgacacca gcggcgtgta cggcgacccg gcggcgcaca tcgacctgaa acaaggcctg
    21661 ccgcacatcc gcactgcatg gctgaacgaa cgcggtgata ccgaaatcct gcccaagctt
    21721 tccagcgaat acggcatcga acgcgcacac gatccgaaaa ccgcccatct gcgcttcaac
    21781 caaatcaccc gcccgcgccg cgccaaagcc ggcggcaacg ttacccagct ccattacgcc
    21841 cgccaaggca ttatcacgcc cgaaatggag tttgtcgcca tacgcgaacg tttaaaatta
    21901 gacgaattgt cccaaaaacc ggaatacgcc aaactcttgg aacagcacgc ggggcaaagt
    21961 ttcggtgcga acatcccgac ccatcccgac caaatcacgc ccgaattcgt gcgccaagaa
    22021 atcgccgccg gacgcgcgat catccctgct aacatcaacc accccgaact cgaaccgatg
    22081 attatcggcc gcaacttccg tgtcaaaatc aacggcaact tgggcaactc cgccgtcacc
    22141 tccagcctga ccgaagaagt cgaaaaaatg gtgtggtcgc tgcgttgggg cgcggacacg
    22201 attatggatt tgtccaccgg cgcgcacatc catgaaacgc gcgaatggat tatccgcaac
    22261 gcgcccgtcc ccatcggcac cgtgccgatt taccaagcgt tggaaaaaac cggcggcatc
    22321 gccgaagatt tgacttggga tttgttccgc gacaccctca tcgaacaggc ggagcaaggc
    22381 gtggactatt tcaccataca cgcgggcgtg ttgctgcgtt atgtgccgat gaccgccaac
    22441 cgcctcaccg gcatcgtctc gcgcggcggt tcgattatgg cgaaatggtg tctggcacat
    22501 catcgggaaa atttcctcta cacgcatttc gacgaaatct gcgaaattat gaaagcgtat
    22561 gacgtatcat tcagcctcgg cgacggcctg cgccccggct gcattgtcga tgccaacgac
    22621 gaatcccagt tctccgaatt gcacaccttg ggtgaattga ccgataaagc gtggaaacat
    22681 gacgtacaag tcatgatcga aggccccggc catgtgccgc tgcaacgcgt caaagaaaac
    22741 atgaccgaag agctgcaaca ctgctttgaa gcaccttttt acacgctcgg cccgctcgtt
    22801 accgacatcg cacccggcta cgaccacatc acctcgggca taggcgcggc caatatcggc
    22861 tggtacggca cggcgatgct ttgttacgtt acccccaaag aacatttggg gctgcccgac
    22921 aaagaagacg tgcgcaccgg catcatcacc tacaaactcg ccgcccacgc cgccgatctc
    22981 gccaaaggct ggccgggcgc acaattacgt gacaacgccc tgagcaaagc gcgtttcgag
    23041 ttccgctggc gcgaccaatt tcgcttaagc ctcgaccctg aacgtgccga gagcttccac
    23101 gacgaaaccc tgcctgccga aggcgcgaaa atcgcccact tctgctcaat gtgcggcccc
    23161 aaattctgct cgatgaaaat cacgcaggaa gtgcgcgact acgccgacaa gcaaaaagcc
    23221 caacggcagg gaatggaaga aaaagcggtc gagttcgtca aaaaaggagc gaagatttac
    23281 agctaaaacg tcaagcaaaa aatgccgtct gaaacccgga aaaaaggctt cagacggcat
    23341 tctttcgctt gtgaaaat
//
LOCUS       DAXHTY010000022        22975 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXHTY010000022 DAXHTY010000000
VERSION     DAXHTY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22975
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.022"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            35..922
                     /gene="prmA"
                     /locus_tag="ACFNMK_000739"
     CDS             35..922
                     /gene="prmA"
                     /locus_tag="ACFNMK_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC6364052.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTAAQECGLKDLAYTGEILEDQDWVR
                     LTQAQFDPIRISERLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGFAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            940..1503
                     /gene="orn"
                     /locus_tag="ACFNMK_000740"
     CDS             940..1503
                     /gene="orn"
                     /locus_tag="ACFNMK_000740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC6364053.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(1754..3037)
                     /gene="hemL"
                     /locus_tag="ACFNMK_000741"
     CDS             complement(1754..3037)
                     /gene="hemL"
                     /locus_tag="ACFNMK_000741"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC6364054.1"
                     /translation="MNRNEILFDRAKAVIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAMREAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPSEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRNVAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(3101..3514)
                     /locus_tag="ACFNMK_000742"
     CDS             complement(3101..3514)
                     /locus_tag="ACFNMK_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6364055.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFLRDQTEEKIVQFAVRGKTT
                     RAEVEARFGRQNPFGCYAYHEVSLPIYNFLPTNFIYMKSERRHWEWCVDYDWEGVVRD
                     YRFTHKVEKDERSVIRDAVGVIRKEAGKSLSQPEK"
     gene            3717..5045
                     /gene="miaB"
                     /locus_tag="ACFNMK_000743"
     CDS             3717..5045
                     /gene="miaB"
                     /locus_tag="ACFNMK_000743"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC6364056.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMVGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKTVSI"
     gene            complement(5130..7037)
                     /gene="dxs"
                     /locus_tag="ACFNMK_000744"
     CDS             complement(5130..7037)
                     /gene="dxs"
                     /locus_tag="ACFNMK_000744"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC6364057.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSENKPAAKPTYTQVF
                     GKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLACE
                     GMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSFLR
                     CVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKGII
                     RREGEKTAFIAFGSMVAPALAVARKLNATVADMRFVKPIDEELIVRLARSHDRIVTLE
                     ENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDGLGLSAEAVERRV
                     RAWLSDRDAAN"
     gene            complement(7122..8039)
                     /gene="xerC"
                     /locus_tag="ACFNMK_000745"
     CDS             complement(7122..8039)
                     /gene="xerC"
                     /locus_tag="ACFNMK_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC6364058.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQGLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVKRGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            8233..9297
                     /gene="fba"
                     /locus_tag="ACFNMK_000746"
     CDS             8233..9297
                     /gene="fba"
                     /locus_tag="ACFNMK_000746"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC6364059.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            9317..10279
                     /locus_tag="ACFNMK_000747"
     CDS             9317..10279
                     /locus_tag="ACFNMK_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC6364060.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCFSLTAALILTACSSGGGGVAADIGAGLADALTAPLDHKDKGLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGKLITLESGE
                     FQVYKQSHSALTALQTEQVQDSEDSGKMVAKRQFRIGDIAGEHTSFDKLPKGGSATYR
                     GTAFGSDDAGGKLTYTIDFAAKQGHGKIEHLKSPELNVELATAYIKPDEKRHAVISGS
                     VLYNQDEKGSYSLGIFGGQAQEVAGSAEVETANGIHHIGLAAKQ"
     gene            10419..11096
                     /gene="tsaB"
                     /locus_tag="ACFNMK_000748"
     CDS             10419..11096
                     /gene="tsaB"
                     /locus_tag="ACFNMK_000748"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC6364061.1"
                     /translation="MQADFNRPVLAIDTGTSYLSLALRADGETHLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRDKIALTAKEQ
                     AERKART"
     gene            11093..11533
                     /gene="rimI"
                     /locus_tag="ACFNMK_000749"
     CDS             11093..11533
                     /gene="rimI"
                     /locus_tag="ACFNMK_000749"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC6364062.1"
                     /translation="MNIRRASPSDCAALAVLDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEDTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            11527..12264
                     /locus_tag="ACFNMK_000750"
     CDS             11527..12264
                     /locus_tag="ACFNMK_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6364063.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            12326..12967
                     /gene="pyrE"
                     /locus_tag="ACFNMK_000751"
     CDS             12326..12967
                     /gene="pyrE"
                     /locus_tag="ACFNMK_000751"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC6364064.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            13041..13475
                     /locus_tag="ACFNMK_000752"
     CDS             13041..13475
                     /locus_tag="ACFNMK_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC6364065.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQEGFNWTIATLFALIVLIMQLSYLVIL"
     gene            13472..14782
                     /gene="argA"
                     /locus_tag="ACFNMK_000753"
     CDS             13472..14782
                     /gene="argA"
                     /locus_tag="ACFNMK_000753"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC6364066.1"
                     /translation="MSAPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFHLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AVTLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGASDGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFTEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            14981..16996
                     /locus_tag="ACFNMK_000754"
     CDS             14981..16996
                     /locus_tag="ACFNMK_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC6364067.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARTLS
                     DGILAQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCAAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPDRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPADCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFTDGKWQEAELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDMPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPKHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRWLGELSPYHNLSDDVPYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(17110..18066)
                     /locus_tag="ACFNMK_000755"
     CDS             complement(17110..18066)
                     /locus_tag="ACFNMK_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6364068.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFTL
                     QRLAQNAAALTAAACPLLQRSFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFVRPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            18302..19264
                     /locus_tag="ACFNMK_000756"
                     /pseudo
     CDS             18302..19264
                     /locus_tag="ACFNMK_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
     gene            complement(20120..20272)
                     /locus_tag="ACFNMK_000757"
     CDS             complement(20120..20272)
                     /locus_tag="ACFNMK_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364069.1"
                     /translation="MRFVVLASFSGRSCNESVIGFDVSLRLTALSLTLSHRERGRVAV
                     ASLFAA"
     gene            20303..20626
                     /locus_tag="ACFNMK_000758"
     CDS             20303..20626
                     /locus_tag="ACFNMK_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6364070.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            20740..22917
                     /gene="tdfF"
                     /locus_tag="ACFNMK_000759"
     CDS             20740..22917
                     /gene="tdfF"
                     /locus_tag="ACFNMK_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
                     /protein_id="HFC6364071.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQKSTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRNQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRRRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT         5235 a         7067 c         6179 g         4494 t
ORIGIN      
        1 aattcaatcc actatatttc caagaaagcc cgttatgccc taccaacaaa tcaccgtcaa
       61 cgtcaacgat gccgtcgccg aacgcctcgc cgacgcgctg atggaacacg gcgcactctc
      121 cgccgccatc gaagatgcct acgccggcac gcaaaacgaa caggcgattt tcggcgaacc
      181 cggtatgccc gccgaacaaa tctggcagca gagcaaagtc atcgccctgt tcggcgaaca
      241 cgacgaagcc gccgccatca tccaaaccgc cgcacaagaa tgcggattaa aagacttggc
      301 atacaccggc gaaatcctcg aagaccaaga ctgggtacgc ctgacccaag cgcaattcga
      361 ccccatccga atttccgaac gcctgtggat taccccctct tggcacgaag cccccgaagg
      421 cactgccgtc aacctacgcc tcgatcccgg cctcgccttc ggcaccggca gccacccgac
      481 cacgcgcctc tgcctcaaat ggctggatac gcaactcaaa aacggcgaaa gcgtcctcga
      541 ctacggctgc ggttcgggca tcctgaccat cgccgccctc aaactcggtg caggtttcgc
      601 cgtcggcgtg gatattgacg aacaagccgt ccgcgccggc aaggacaacg ccgaacagaa
      661 caacgtcgat gcacaattct tcctgcccga cggtctgcct caagggcaat tcgacgtagt
      721 tgtcgccaac atcctcgcca accctttacg tatgctcggc gaaatgctcg ccgcccgcac
      781 caaacaaggc ggccgcatcg tgttatccgg tttgttggac gaacaagccg aagaactcgg
      841 cggcatttac agccaatggt tcgatctcga cccggcggaa accgaagaag gctgggcgcg
      901 cttgagcggg acaaaacgct gaaacggaaa ggaaacaccg tgcaggacaa aaacaacctc
      961 tgctggctcg atatggaaat gacggggctt aatcccgaaa ccgaccgcat tatcgaagtc
     1021 gcgatgatta ttaccgactc ggatttgaat gtgttggcgc aatccgaagt ttacgccgtc
     1081 caccaaagtg acgagctgct cgacaatatg gacgagtgga acaccgccac acacggcagg
     1141 acggggctga cgcagcgcgt acgcgaatcg tcgcataccg aagccgaagt cgaacagaaa
     1201 ctgctggact ttatgtcgga atgggtaccc ggacgcgcca cgccgatgtg cggcaactcc
     1261 atccaccaag accggcgttt tatggtcaaa tatatgccga aactggaaaa ctacttccac
     1321 taccgcaacc ttgacgtttc cacgctgaaa gaactcgcca aacgctggaa tccgcccgtt
     1381 gccaaaagcg tggtcaaacg cggttcgcac aaggcattgg acgacatttt ggagagcatc
     1441 gaagaaatgc gccactaccg cgaacacttt ctgatttccg ccccgagagc cgaagcgcaa
     1501 taagaaacaa acaatgccgt ctgaaacgct gtttgcattt cagacggcat ttttacagca
     1561 gattgaaatc aaaaatatac acgcccgtca ttcccgcgca ggcgggaatc cggaaggtcg
     1621 ggcttgccgt tattttcaat cattacagaa actgaaaggt ctggattccc gcctgcgtgg
     1681 gaatgacggc ggtgtgcatt cttacttcaa tctgccaaac agatcgaaca gagaaactct
     1741 gtccgtcaaa acatcatgca gccatcgcct tgaacacttc aaccgcaacc gcaaccgttt
     1801 cgtcaatcag ctcgggcgta tgcgcggcag aaacgaaacc tgcttcataa gcggacgggc
     1861 cgaaggcgac attgcggtcg agcatcccgt ggaaaaactg tttgaagcct tcaatattgg
     1921 aacgcgccat atcggcataa tttcgcggcg cgtgtgcggc gaaatacaga ccgaacatac
     1981 cgcccacgct gtcggcggtg aactcgatgc ccgccgcatc cgctgccgtc cgaaaacctt
     2041 gaaccaactg ttcggtacgc gccgtcaggt tttcataaaa cccttcgcgg cggatgattt
     2101 ccagcgtttt caaaccggcg gcgacggcaa tcgggttgcc cgacaaggtg cctgcctgat
     2161 acacgccgcc cagcggggaa atacattcca taatgtcttt gcgtccgccg aacgcggcaa
     2221 gtggcatacc gccgccgatg actttgccca tcgtggtcag gtcgggcgtg atgccgtgca
     2281 gcgattgtgc gccgccgagc gcgacgcgga aaccggtcat cacttcgtcg taaatcaaca
     2341 ctgcgccgtg tttttcagtc aattcgcgca gggctttgac gaaggcttca gacggcctaa
     2401 ccagattcat attgccgaca aaaggctcca aaatcacaca ggcaatgtca ttgccgcttt
     2461 gagcaaaggc ttcttcgagt tgggcgatgt tgttgtattc gaggactaaa gtgtgtttgg
     2521 taaagtcggc aggcacaccg gcggaagagg gattaccgaa ggtgagcaga ccgctgcctg
     2581 ctttcaccaa caggctgtcg gaatggccgt ggtagcagcc ttcaaatttg atgattttgt
     2641 cacggccggt aaagccgcgt gccagacgga tggcggtcat ggtggcttcg gtaccggagc
     2701 tgacaaggcg cagccgttcg acggacggca tgattttggc gatttcttcg gcaatgacga
     2761 tttcgccttc ggtaggcgcg ccgaacgaca aaccgcccaa ggcagcttcg cgcatggctt
     2821 cgatgacttc ggggtgcgcg tgtccgacaa tcgcgggccc ccacgaaccg acatagtcgg
     2881 tgtagcgcgt gccgttttcg tcccaaacat acgcgccttc ggcttttttg ataaagcgcg
     2941 gcacgccgcc gacgctgccg aatgcgcgga ctggcgaatt cacaccgccg gggatgaccg
     3001 ccttggcgcg gtcgaataaa atttcgttac ggttcatatt tatcctcaaa tgccgcctga
     3061 acggcaggtt tcgggcttag aagcagaaag tcccatttta tcatttttca ggttgcgaca
     3121 aggatttgcc tgcttctttg cggatgacgc caaccgcatc ccggatgacg gaacgctcgt
     3181 ctttttccac tttatgtgta aagcggtagt cccggacaac tccttcccag tcgtaatcca
     3241 cacaccactc ccaatgtcgg cgttctgatt tcatataaat gaaattggtc ggcaaaaaat
     3301 tataaatcgg caggctgacc tcgtgatagg cataacagcc gaacggattt tgccgcccga
     3361 aacgcgcttc cacttccgcc cgggtcgtct tgcccctaac cgcaaactgc acgatttttt
     3421 cttccgtctg atcacgcagg aactgatgtt ccctgtcgcc gaaattgctg gtacacgcac
     3481 acagcagcaa tgccgcccac acagccggtt tcatacacac ctcccattaa agccaaacat
     3541 tatacagccg tcccgaccga ttaaattcat attttaaaac aatattctgt ttacaaaacc
     3601 cacatcgtgc tataatccgc accgattttc agacggcatc gtcatgccgt ctgaaatttt
     3661 ttcattccaa caacaatcag ccccgcgatt acggctgcct gagaaagaca caaaccatga
     3721 aaaaagtatt tatccgcacc ttcggctgcc agatgaacga atacgacagc gacaaaatgc
     3781 tcgccgtcct cgccgaagaa cacggcggca tcgaacaggt tacccaagcc gacgaagccg
     3841 acatcatctt gttcaacacc tgctccgtgc gcgaaaaagc acaggaaaaa gtcttctccg
     3901 atttggggcg cgtgcgtccg ctcaaagaaa aaaaccccgg cctcatcatc ggcgttgccg
     3961 gctgcgtcgc ctcgcaagaa ggcgaaaaca tcatcaaacg tgcgccttat gtggacgtgg
     4021 ttttcggccc gcaaacactg caccgcctgc ccaaaatgat tgtggacaaa gaaaccagcg
     4081 ggctgtcgca agtcgatatt tccttccccg aaatcgaaaa attcgaccac cttccgcccg
     4141 cccgcgtcga aggcggcgcg gcattcgtgt cgattatgga aggctgttcc aaatactgct
     4201 ccttctgcgt cgtcccctac acgcgcggcg aagaattctc ccgcccgctc aacgacgtat
     4261 tgacagaaat cgccaacctt gcccagcaag gcgtgaaaga aatcaacctc ttaggccaaa
     4321 acgtcaatgc ctatcgcggc gaaatggacg acggcgaaat ctgcgacttc gccaccctgc
     4381 tgcgtatcgt ccacgaaatc cccggcatcg aacgtatgcg cttcaccacc agccacccac
     4441 gcgagtttac cgactcgatt atcgagtgct accgcgacct gccgaagctg gtttcccacc
     4501 tgcacctgcc gattcaaagc ggttccgacc gcgtattgag cgcgatgaaa cgcggctaca
     4561 ccgctttgga atacaaatcc atcatccgca aactgcgcgc catccgtcct gatttgtgcc
     4621 tcagctccga ctttatcgtc ggcttcccgg gcgagaccga gcgcgagttc gagcaaacct
     4681 tgaaactggt gaaagacatt gccttcgact tgagctttgt ctttatttac agtccgcgcc
     4741 ccggcacgcc tgccgccaac ctgccggacg acacgccgca cgaagaaaaa gtgcgccgcc
     4801 tcgaagcttt gaacgaagtc atcgaagccg aaaccgcgcg catcaaccaa accatggtcg
     4861 gcacggttca acgctgcctg gtcgaaggta tctccaaaaa agaccccgac caactgcaag
     4921 cccgcaccgc caacaaccgc gtcgtcaact ttaccggcac gcccgatatg atcaaccaaa
     4981 tgatcgattt ggaaatcacc gaggcctaca ccttctccct gcgcggcaaa accgtcagca
     5041 tttgatttcc cccgccgcaa aaatgccgtc tgaagcgttt cagacggcat tttgccttgt
     5101 atcggcagac gatggcgcgg ccaggcggct taatttgccg catcccgatc cgacaaccac
     5161 gcgcgcacac gccgttccac cgcttcggca ctcaaaccca aaccgtccaa aagttttttc
     5221 ggatcgccgt gtccggttac ggtatcggca acgcccaaaa gcaaaacggg tttgcagatg
     5281 ccgtgtttcg ccaatacttc cagcaccgcg ccgcctgcgc cgccctgttc ggcgttttct
     5341 tcaagggtaa cgatgcggtc gtggcttcgg gcaaggcgga caatcaactc ttcgtctatc
     5401 ggtttgacaa agcgcatatc ggcgacggtg gcgttcagtt ttctggcgac cgccaatgca
     5461 ggggcgacca tactgccgaa ggcaatgaat gcggttttct caccttcgcg gcggataatg
     5521 cccttgccga tttccacggt ttccatgccg tctgaaaccg gcgcgcccgt acccgtgccg
     5581 cgcggatagc ggacggcggc gggcgcgtct gcctgatagc aggtcgaaag caacaggcgg
     5641 cattcgtttt catcgctcgg cgcggcgaca atcatgttcg gcacgcagcg caaaaagctc
     5701 aaatcgtaca gaccggcatg ggtcgggccg tccgcgccga cgatgcccgc gcggtcgacg
     5761 gcaaacaaaa cgggtaggtt ttgcagggcg atgtcgtgca ccagttggtc gtaggcgcgt
     5821 tgtaaaaagg tggaataaat cgccacgacg ggcttcatcc cttcgcaagc caaaccgccg
     5881 gcaaaggtaa cggcgtgctg ctcggcaatg ccgacatcga aataacggtc ggggaatcgt
     5941 tgttcaaact ccaccagtcc gctgccctcg cgcatcgcgg gcgtgatggc gatcaaacgc
     6001 tcgtctgccg ccgcccggtc gcacagccat ttgccgaaca cttgggtata ggtcggtttg
     6061 gcggcaggtt tgttttcaga cggcatttgc gccgcgcttt ctttgggcag gttggcgacg
     6121 gcgtggtatt tgacgggatc gttttcggcg agtttgtagc cgttgccttt tttggtaatg
     6181 acgtgcagca actgagggcc tttgcggctg cgcaagtctt tcaatacgtc caccagattt
     6241 tcgacgttgt gtccgtccac ggggccggtg tagcggaagc cgaagttttc aaacaaagac
     6301 agtgactgtt tggcgtgttc ggcttcttcg gcaagggttt tgattttatg ttcgactttt
     6361 tgggcaaact ccatcgcgcc gggtattttg tctaatacct tgcccgtttg cgctttgacg
     6421 gtactcaaca gtccgtgcat atcgcgcacg acgttgctgg caaggtattt gggcaacgca
     6481 ccgacgttgg gggaaatcga catttcgttg tcgttgagga cgaccagcaa atccacatcc
     6541 atatcgcccg cgcaattcaa ggcttcaaac gcctgccccg ccgtcatcgc gccgtcgccg
     6601 atgatggcga cgctgcggcg gtcgctgccc aagagtttgt ctgccgccgc catgcccaac
     6661 gccgcgccga tggatgtgga agaatgccct acgccgaacg cgtcgtattc cgactcgctg
     6721 cgttttggga agccagctaa accgccgtat tggcgcatgg tgtgcatctg gtttttcctg
     6781 cctgtcagga ttttgtgcgg atagctttgg tgtccgacat cccataccag cttgtcttcg
     6841 ggcgtatcgt acacatagtg cagggcgatg gtcagttcga ccgcgcccaa attgctggcg
     6901 aaatgcccgc cggtctgccc gacagattcc agcagaaagg cgcgcaactc gccggcaagg
     6961 cgcggcagct gttttttgtc cagacggcgc aaatcttgcg ggctgtcaat caggtcgagt
     7021 agggggcttg ggttcatggt gtgtcttttt tatgtgtcgt ccgggtgcaa cggccaatta
     7081 tatatcaaga gcgtgcggct gacggctgat tttgccgtat gtcattcgtc ctgccgcttg
     7141 gcgcgcgggt gggcttcgtc atacaggcgg gcgatgtggt cgaaatcgag cttggtataa
     7201 atctgcgtgg tcgaaaggct gctgtgcccg agcagctcct gcaccgccct gatgtcgcgc
     7261 gaagcctgca acaggtgtcc ggcgtagctg tggcgcatca tatgcggcga aacgtgcctg
     7321 ccgtcgccgt tttgcgccgc ccattgcgcc aaacgttttt ggatttggcg ttggctcagg
     7381 cgcgtgccgt tcctgccggt aaacagggct ttgccgtccg atgccgtctg acgcagcggc
     7441 agatagtttt tcagggcttc cacgcttttg ccggtcagcg gcacctgccg ctgcttgcgc
     7501 cctttgccgg taacgtgtac ccacgcctcg tccaaataga catcatctgc attcaagccg
     7561 tgtatctcgc tcacgcgcaa accgctgccg tacatcagct cgaacagggc gtgatcgcgc
     7621 accgccagcg ggtcgccgcc gtccacgggc aaatccagca tccggttcag ccattcctgc
     7681 ggcagtgctt tgggtacgcg ctcgggctgc ttcggcggtt tgatgtcggc ggtcgggtcg
     7741 gtatgcagca gaccgcgttt gaccagccag gcgcaatact gccgccaaga cgacagcttg
     7801 cgcgccagcg tccgcgcatt caaaccgcgc tgcgacagcc gccgcaacgc cgccgtaaaa
     7861 tcgcgccgcg acaagccctg cggcacgccg ccttcagctt cagacggcat ttgtgccaac
     7921 agcgcaaaca gttcttccaa atcgcgccgg tatgccgcca ctgtgtgctc cgacttgccc
     7981 tcgcgcacga tgttttccaa ataagcgtca aaatgcgccg caaacccgtc caaaaccata
     8041 accgccccac acaaatatca aaaaaacagt gaaaacatta tcataaacga cacaccaaaa
     8101 ccccgaactg cccaaaatcc gacccgtccg cccgccgtta tcgttaacat aaaatcaatg
     8161 agccgtttat ggttttttgc cgcaaaaaac attataatcc gccttattta cctattgccc
     8221 aaggagacac aaatggcact cgtatccatg cgccaactgc ttgatcatgc tgccgaaaac
     8281 agctacggcc tgcccgcgtt caacgtcaac aacctcgaac aaatgcgcgc cattatggaa
     8341 gccgccgacc aagtcaacgc gcccgtcatc gtacaggcga gtgcaggtgc gcgcaaatat
     8401 gcgggtgcgc cgtttttacg ccacctgatt ctggcggcag tcgaagaatt tccgcacatc
     8461 cccgtcgtga tgcaccaaga ccacggcgca tcgcccgacg tgtgccaacg ctccatccaa
     8521 ctgggctttt cctccgtgat gatggacggt tcgctgatgg aagacggcaa aaccccttct
     8581 tcttatgaat acaacgtcaa cgccacccgt accgtggtta atttctccca cgcttgcggc
     8641 gtatccgtcg aaggcgaaat cggcgtattg ggcaacctcg aaaccggcga agcaggcgaa
     8701 gaagacggtg taggcgcagt gggtaaactt tcgcacgacc aaatgctgac cagcgttgaa
     8761 gatgccgtgc gtttcgttaa agataccggt gttgacgcat tggcgattgc cgtcggcacc
     8821 agccacggcg catacaaatt cacccgtccg cccacaggcg acgtattgcg tatcgaccgc
     8881 atcaaagaaa tccaccaagc cctgcccaat acacacatcg tgatgcacgg ttccagctcc
     8941 gttccgcaag aatggctgaa agtcatcaac gaatacggcg gcaatatcgg tgaaacctac
     9001 ggcgtgccgg ttgaagaaat cgtcgaaggc atcaaacacg gcgtgcgcaa agtcaacatc
     9061 gataccgact tgcgccttgc ttccaccggc gcggtacgcc gctaccttgc cgaaaacccg
     9121 tccgacttcg atccgcgcaa atatttgagc aaaaccattg aagcgatgaa gcaaatctgc
     9181 ctcgaccgct acctcgcgtt cggttgcgaa ggtcaggcag gcaaaatcaa accggtttcc
     9241 ttggaaaaaa tggcaaaccg ttatgccaag ggcgaattga accaaatcgt caaataacag
     9301 gttgcttgta aacaaaatgc cgtctgaacc gttgttcgga cggcatttga tttttgcttc
     9361 tttgacctgc ctcattgatg cagtatgcaa aaaaagatac cataaccaaa atgtttatat
     9421 attatctatt ctgcgtatga ctaggagtaa acctgtgaac cgaactgcct tctgctgctt
     9481 ttctctgacc gccgccctga ttctgaccgc ctgcagcagc ggagggggcg gtgtcgccgc
     9541 cgacatcggt gcggggcttg ccgatgcact aaccgcaccg ctcgaccata aagacaaagg
     9601 tttgcagtct ttaacgctgg atcagtccgt caggaaaaac gagaaactga agctggcggc
     9661 acaaggtgcg gaaaaaactt atggaaacgg cgacagcctt aatacgggca aattgaagaa
     9721 cgacaaggtc agccgcttcg actttatccg tcaaatcgaa gtggacggga agctcattac
     9781 cttggagagc ggagagttcc aagtgtacaa acaaagccat tccgccttaa ccgcccttca
     9841 gaccgagcaa gtacaagact cggaggattc cgggaagatg gttgcgaaac gccagttcag
     9901 aatcggcgac atagcgggcg aacatacatc ttttgacaag cttcccaaag gcggcagtgc
     9961 gacatatcgc gggacggcgt tcggttcaga cgatgctggc ggaaaactga cctatactat
    10021 agatttcgcc gccaagcagg gacacggcaa aatcgaacat ttgaaatcgc ccgaactcaa
    10081 tgtcgagctt gccaccgcct atatcaagcc ggatgaaaaa cgccatgccg ttatcagcgg
    10141 ttccgtcctt tacaaccaag acgagaaagg cagttactcc ctcggtatct ttggcgggca
    10201 agcccaggaa gttgccggca gcgcggaagt ggaaaccgca aacggcatac accatatcgg
    10261 tcttgccgcc aagcagtaac cattgtgaaa atgccgtccg aacacaataa tttaccgttc
    10321 ggacggcatt tttgcgccat accgccctag gacacgacgg cacggcaggc aagctgattt
    10381 cactatataa tgccgtctga accaacgaga accataccat gcaagctgat tttaaccgtc
    10441 ccgtcctggc catcgatacc ggtacttcct atttgtcgct cgcgctgcgc gccgacggcg
    10501 aaacccacct gttccatcaa gaggccggca gccgccaatc cgaactgatt ctgccggaaa
    10561 tccgcaccct attccgaaat gcgggcatta ccgccgccga tttgggggcg attgtttatg
    10621 cgaaaggccc cggcgcgttt acgggactgc gtatcggcat cggcgtggcg caaggtttgg
    10681 caacgccgtt tgatacgcct ttaatcggca tccccacgct cgatgccgcc gcctcgctgc
    10741 cgccgccgca aagctgcata ctcgccgctg cggacgcgcg tatgggcgaa gtgttttatg
    10801 cgtggttcga cacgctgaac cgccgccgtt tgagcgatta tcaggtcggg cgggcggcag
    10861 acatccggct gccggaggga tgtgcctttt cagacggcat aggcagcgcg ttcgcgttgg
    10921 aagaaacgcc gccgttttca ggcaaacccg atatgccgac cgccgccgac ttcctcaatc
    10981 tggcggctga aggcggtttt cccgccgtcg ctgcggcaca cgccgagctg ctctacgtcc
    11041 gcgacaaaat cgccttgacc gccaaagaac aggcggaacg gaaagcccga acatgaacat
    11101 ccgccgcgcc tcaccgtccg actgcgccgc actcgccgtg ctcgatgccg tctgcaaccc
    11161 gtccgcgtgg acgcaacgcc aatttgagtc cgcattggtt tcgccgtccg aacaggtttt
    11221 cctcgcggaa aaagacggcg ggattgccgc ctttatcgtt tggcagaacc tgcccgacga
    11281 atccgaactg cacctgattg ccaccgcgcc cgaatgccgc cgccgtggcg ttgcgtccgc
    11341 cctgctcgaa tattggttcg cacatctgcc cgaagacacg caacgcctgc tgctcgaagt
    11401 ccgtgcaggc aacaccgccg cacaggcact gtacgcggcg cacggcttta ccgatgcggg
    11461 caggcggaaa aactattacc gtacagccga cggtaaaacc gaagatgctg tcttaatgga
    11521 gaaaatatgt taagcgcgcg ctacctccac ctgcacgaag ctttgggttt aggcccgatg
    11581 tggctgaaac aggccgccgc cgtcctgccg cccaaaaaca cacccgcaac cccggcacag
    11641 gcacgtcctc aaaccgtccg cgccgccccg atccgccctt cccaacccca taacggtcag
    11701 gcgcggctcg aaacgatgaa agcgttggaa accgccgccg tacatacgcg caaacccgcg
    11761 cctgaaaccg aaacgccccc gcccggcctt tcagacggca tcgcccccgt tcccgccgct
    11821 tcgggcataa ccaaacttgc cgtcgtcagc ctttgcccac cgattgagga tgcggtttac
    11881 gggcaactgt tctacggcaa agcgggtgtt ctgcttgaca acatactcaa agccgtagga
    11941 ctggatgccg cctatgtcca caaaacctgt tgggtgaaaa ccgccgccgt cggcaacccg
    12001 atgccgtctg aagcggcaat cgaaaccgcg acggtttccg tcatgcagga actcgacggc
    12061 tgccgcgccc ccgccgtcct attcctcgga caggcttttg tccagcctga acggcaaacg
    12121 atgattgaaa ctttgtgcgg cagccgtccc ttcttcatca tcgaccatcc cgcccggctg
    12181 ttgcgccaac ccgaacttaa agcccgcgcc tggcaggtgt tgaaacagtt gaaacgcgcc
    12241 ttgcggcaag gcggcggcag ttgaagcgca ccgcacgggg cggtagaatc gcaactgcgt
    12301 cccaatatct gacagaaagc acaaaatgac cgatttccgc caagatttcc tcaaattctc
    12361 cctcgcccaa aatgttttga aattcggcga attcaccacc aaagccggac ggcggtcgcc
    12421 ctatttcttc aatgccggcc tctttaacga cggcttgtcc acgctgcaac tggcaaaatt
    12481 ttacgcacaa tccatcattg aaagcggcat ccgattcgat atgctgttcg gccccgccta
    12541 caaaggcatt attttggcgg cggcaaccgc gatgatgctg gcggaaaaag gcgtgaacgt
    12601 cccgtttgcc tacaaccgca aagaagccaa agaccacggc gaaggcggcg tgttggtcgg
    12661 cgcgccgctt aaagggcgcg tgctgattat cgacgacgtg atttccgccg gcacatccgt
    12721 acgcgaatcg atcaaactga ttgaagcgga gggtgcaacc cccgccggtg tcgccatcgc
    12781 gctcgaccgc atggaaaaag gcacgggtga attgagcgcg gttcaggaag tggaaaaaca
    12841 atacggtctg cccgtcgccc ccatcgccag cctgaacgat ttgtttatcc tgttgcaaaa
    12901 caatcccgaa ttcggacagt tcctcgaacc cgtccgagcc taccgtcggc agtacggcgt
    12961 agaataaaaa caaagcatat gccgtctgaa ccgccttacg cctcagacgg catcaaacct
    13021 gacacacacg aggaaatacc atgcccgcct gtttctgccc ccactgcaaa acccgtctct
    13081 gggtcaaaga aacccaactc aatgtcgccc aaggcttcgt cgtctgccaa aaatgcgaag
    13141 gactgtttaa agccaaagac catctggcaa gcacgaaaga acccatattc aacgatttgc
    13201 ccgaggctgt ttcggatgtc aaactcgttc accgtatcgg cacgcgcgcc atcggcaaga
    13261 aacagatttc ccgtgacgaa atcgccggca tcctcaacgg cggcacaacc cagcccgata
    13321 ttccgcccgc aaccgccgcc acccctgctg ccgcaccgca ggttaccgta ccacccgccg
    13381 cgcccgcccg tcaggaaggg ttcaactgga cgattgcaac cctgtttgcc cttatcgtcc
    13441 tcattatgca gctttcctac ctcgtcatcc tatgagcgcg cccgacctct ttgtcgccca
    13501 cttccgcgaa gccgccccct acatccgcca aatgcgcggc aaaacgctgg tcgccggcat
    13561 agacgaccgc ctgctcgaag gcgatacctt aaacaagctc gccgccgaca tcgggctttt
    13621 gtcgcaactg ggcatcaggc tcgtcctcat ccacggcgcg cgccacttcc tcgaccgcca
    13681 cgccgccgcg caaggccgca cgccgcatta ttgccggggc ttgcgcgtta ccgacgaaac
    13741 ctcgctcgaa caggcgcagc agtttgccgg caccgtccgc agccgttttg aagccgcatt
    13801 gtgcggcagc gtttccgggt tcgcgcgcgc gccttccgtc ccgctcgtat cgggcaactt
    13861 cctgaccgcc cgtccgatag gtgtgattga cggaaccgat atggaatacg cgggcgttat
    13921 ccgcaaaacc gacaccgccg ccctccgttt ccaactcgac gcgggcaata tcgtctggct
    13981 gccgccgctc ggacattcct acagcggcaa gaccttccat ctcgatatgc ttcaaaccgc
    14041 cgcctccgtc gccgtctcgc ttcaggccga aaaactcgtt tacctgaccc tttcagacgg
    14101 catttcccgc cccgacggca cgctcgccgt aaccctctcg gcacaggaag cgcaatcgct
    14161 ggcggaacac gccggcggcg aaacgcgacg gctgatttcg tccgccgttg ccgcgctcga
    14221 aggcggcgtg catcgcgtcc aaatcctcaa cggtgcctcc gacggcagcc tgctgcaaga
    14281 actcttcact cgcaacggca tcggcacgtc cattgccaaa gaagccttcg tctccatccg
    14341 gcaggcgcac agcggcgaca tcccgcacat tgccgccctc atccgcccgc tggaagaaca
    14401 aggcatcctg ctgcaccgca gccgcgaata cctcgaaaac cacatttccg aattttccat
    14461 cctcgaacac gacggcaacc tgtacggttg cgccgcccta aaaaccttta ccgaagccga
    14521 ttgcggcgaa atcgcctgcc ttgccgtctc gccgcaggca caggacggcg gctacggcga
    14581 acgcctgctt gcccacatta tcgataaggc gcgcggcata ggcataagca ggctgttcgc
    14641 actgtccaca aataccggcg aatggtttgc cgaacgcggc tttcagacgg catcggaaga
    14701 cgagttgccc gaaacgcggc gcaaagacta ccgcagcaac ggacggaact cgcatattct
    14761 ggtgcgtcgc ctgcaccgct gaccgcaacg gaaagccgcc gcagaaatgc cgtctgaacc
    14821 ccgtttcaga cggcatttcc ccgattatat agtggattaa caaaaaccag tacggcgttg
    14881 cctcgccttg ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
    14941 taatccacta taaagacctg cccaaccctc aaggaccccg atgaaatcct accccgaccc
    15001 ctaccgccat tttgaaaacc tcgattccgc cgaaacgcaa aacttcgctg ctgaagcgaa
    15061 tgccgaaacg cgcgcgcgtt ttttagaaaa cgacaaggcg cgcacgctgt cggacggcat
    15121 tttggcgcag atgcaggaca cgcggcagat tccgttttgt caggaacacc gcgcgcggat
    15181 gtaccatttc catcaggacg cggagtatcc gaagggcgtg taccgcgtgt gtgcggcggc
    15241 gacgtatcgt tccggctatc ccgagtggaa aatcctgttt tcagtggcgg atttcgacga
    15301 gctgctcggc gacgatgtgt atttgggcgg cgtgtcgcac ttggtggaaa agcccgaccg
    15361 tgcgttgtta acactgagca aatcgggcgg cgatacggcg tacacgctgg aagtggattt
    15421 ggaagcaggg gagttggtag aaggcggttt tcactttccg gcaggcaaaa accatgtgtc
    15481 gtggcgcgat gaaaacagcg tgtgggtgtg tccggcctgg gacgagcggc agttgaccga
    15541 atcgggctat ccgcgcgagg tgtggctggt ggaacgcggc aaaagtttcg aggaaagcct
    15601 gccggtgtac caaatcggcg aagacgggat gatggtgaac gcgtggcgtt acctcgatcc
    15661 gcagggttcg ccgattgatt tgattgaagc gtctgacggt ttttacacca aaacctattt
    15721 gcaggtgtcg gccgaaggcg aggcgaaacc gttaaacctg cctgctgatt gcgatgtggt
    15781 cggctatctg gcggggcatc ttttgctgac gctgcgcaag gactggcacc gcgcgaacca
    15841 aagctatccg agcggcgcgc tggtagcggt gaaactgaat cgcggcgaaa tcggggcggc
    15901 gcagcttttg tttgcgcctg atgaaacgca ggcattggaa agcgtggaaa cgaccaagcg
    15961 ttttgtcgtg gcgagtctgc tggaaaacgt acaaggccgt ctgaaagcgt ggcgttttac
    16021 cgacggcaaa tggcaggaag ccgaactgcc gcgcctgccc tcgggcgcgt tggaaatgac
    16081 cgaccaaccg tggggtggcg acgtggttta ccttgccgcc agcgatttca ccacgccgct
    16141 gacgctgttt gcgctggatt tgaacgtgat ggaactgacc gtcatgcgcc gtcagccgca
    16201 gcagttcgat tcagacggca tcaacgtgca gcagttttgg acgacttcgg ctgacggcga
    16261 gcgcattcct tatttccacg tcggcaaaaa cgccgcgccc gacatgccga cgctggtcta
    16321 tgcctacggc ggtttcggca ttcccgaatt gccgcattat ctgggcagca ttggcaaata
    16381 ttggctggaa gagggcaatg cctttgtatt ggcgaacatc cgcggcggcg gcgagttcgg
    16441 cccgcgctgg catcaggcgg cgcagggaat cagcaaacac aaaagcgtcg acgatttgct
    16501 tgccgtcgtg cgcgatttgt ccgaacgcgg catgagttcg cccaaacaca tcggtttgca
    16561 gggcggcagc aacggcggcc tgattaccgc cgctgccttt gtgcgcgagc cgcaaagcat
    16621 cggcgcgctg gtgtgcgaag tgccgctgac cgacatgatc cgttatccgc tgctgtccgc
    16681 cggttcaagt tggacggacg aatacggcaa tccgcagaaa tacgaagtct gcaaacgctg
    16741 gctgggcgaa ttgtcgccgt atcacaatct ttcagacgac gtgccatacc cgcccgcgct
    16801 tattaccacc agcctcagcg atgaccgcgt ccatcccgcc cacgcgctca agttctacgc
    16861 caaactgcgc gaaacctcgc cgcaatcttg gctctactct ccggacggcg gtggccatac
    16921 cggcaacggc acgcaacgtg aatccgccga cgaactcgcc tgcgtcttgc tgtttttgaa
    16981 agagtttttg ggctaaggtc ggggagcggc actgccgccg cgaatgaaaa aaggtcgtct
    17041 gaaactgctt tttcagacga ccttttttaa tggttgtttc aaatcaaaat atctatgccg
    17101 ccggccccat cagcacttct tcacatccga aggcaaaaat ccgtaatgcc gtctgaacgc
    17161 ttcgttgaac cgtcccgcgt ggcggtagcc gcaaaagtgc atggcggctt ggacggtgct
    17221 gccggattcg atgagggcga gcgcgtgttc cagccgcagg cggcgcaggc atccggcgac
    17281 ggtttcgccg gtttgcgctt tgaaatagcg tttcaggtag cattcgttca gtccgacgcg
    17341 gcgggcgatt tcggcgatgg tcagcggacg gacgaattcg tgttgcagga tgtcggcggc
    17401 ttcgtctatg cgccgacggc ggtaaccgtt gtcgtggcgg cggaaggtga agcgcaataa
    17461 tcgggcggag agttccagcg cggcggcttc gtcggcaagc aggccgaagc cgtccgattc
    17521 gaacgagcgt tgcagcagtg ggcaggccgc cgccgtcagt gccgctgcgt tttgcgccag
    17581 ccgttgcagg gtgaatcggc ctatcgtttg cggcgaaaac aggcgttcgt ccagcaagcc
    17641 ttcgtcgtgc cagcggcgca gtttttccag cgaaaaatcc aaatgcagcg cgcacatgcc
    17701 gctgttgtcg ggcaacaggg tttcggatac gtccgccaac tcgccgcgta ccagccagat
    17761 ttcgccggca gacgggcggt attccctgcc gcccatttgt aaccggttct gccccgacac
    17821 catgacgaac aaggcgcagt tgtggctgaa attgtggatt tcggtgggaa acgcgcccgt
    17881 tccgccgccg cgcatccgcg acaaggtgat gcccgagtcg aagcggttga tgcacatttc
    17941 cagatgcaaa ccgggctgtt ttgcctgcgc aatgagcgcg ctgtcggaac agccgtccaa
    18001 cgcccagccg gatttatcgg agcggacata ggtttggtac tggcggtaga tggcggcggt
    18061 gttcatgatt ggataggaac gagttgtcta acaaatgaat taaataggaa ttattaccaa
    18121 taatcaagcg cagggattgg ttgaaacgga aaaggtcgtc tgaaagggtg tttcagacga
    18181 ccttttccgt atcgggaatt tgttttgccg tatcgggaat tttgcgtttt gcggcgtggt
    18241 ttctgcaggt tgtttgctta ataataaaca ttcttattcg tatgcaaagg aaccgcacac
    18301 cgtgaaaccg cgtttttatt gggcagcctg cgccgtcctg ctgaccgcct gttcgcccga
    18361 acctgccgcc gaaaaaactg tatccgccgc atccgcatct gccgccacac tgaccgtgcc
    18421 gaccgcgcgg ggcgatgccg ttgtgccgaa gaatcccgaa cgcgtcgccg tgtacgactg
    18481 ggcggcgttg gatacgctga ccgaattggg cgtgaatgtg ggcgcaacca ccgcgccggt
    18541 gcgcgtggat tatttgcagc ctgcatttga caaggcggca acggtgggga cgctgttcga
    18601 gcccgattac gaagccctgc accgctcaat cctcagctta tcattaccgg cgggccgggc
    18661 gcggaagcgt atgaacagtt ggcgaaaaac gcgaccacca tagatctgac ggtggacaac
    18721 ggcaatatcc gcaccagcgg cgaaaagcag atggagacct tggcgcggat tttcggcaag
    18781 gaagcgcgcg gcggaattga aggcgcagat tgacgcactg ttcgcccaaa cgcgcgaagc
    18841 cgccaaaggc aaaggacgcg ggctggtgct gtcggttacg ggcaacaagg tgtccgcctt
    18901 cggcacgcag tcgcggttgg caagttggat acacggcgac atcggcctac cgcctgtaga
    18961 cgaatcttta cgcaacgagg ggcacgggca gcctgtttcc ttcgaataca tcaaagagaa
    19021 aaaccccgat tggattttca tcatcgaccg taccgccgcc atcgggcagg aagggccggc
    19081 ggctgtcgaa gtattggata acgcgctggt acgcggcacg aacgcttgga agcgcaagca
    19141 aatcatcgtc atgcctgccg cgaactacat tgtcgcgggc ggcgcgcggc agttgattca
    19201 ggcggcggag cagttgaagg cggcgtttga aaaggcagaa cccgttgcgg cggggaaaga
    19261 gtagggagtc gtctgaaaac ggagcttccg aaggaagcgg ggggtttctg cgaagctaaa
    19321 gtgcggtttc aacgaattga aaagcagcct gtattttgaa aataccgctc aagcaaacct
    19381 acggtttgcc gccctctccc tagccctctc ccacagggag aggggattgg ggtgcaggct
    19441 gtctttaagc ttcaggcgat ttttaactct tgttgaagct gcgatttcag aagctccgtt
    19501 ttagcttcgc agaaactccg cttccttcga aagctccgtt ttcagacgac cttttggagt
    19561 accgcaggca cacgcatcga acggctgaat caaagttcag accgatggca gcccgcaccc
    19621 gagtttatgc ggcaaacagc gaggctacgg caacccgccc cctctccctg tgggagaggg
    19681 ttagggagag ggcagtaagc cgtaggctta catcaaagtc gataacgctt tcacaaagcc
    19741 tgtgtgcact gaaaactgaa aggtcatctg aaaacggagc tttcgaagga agcagagttt
    19801 ctgcgaagct aaaaccgaag ctgcaaaaaa tcgaaaagca gcctgcacgt tgaaaatgcc
    19861 gcccaagcaa acctgcggtt tgccgccctc tccctagccc tctcccacag ggagagggga
    19921 ttgggatgca ggctgtcttt aaggttcagg caaattttta acttcgttga aactgcgatt
    19981 tcagaagctc cgttttagct tcgcagaaac tccgcttcct tcgaaagctc cgttttcaga
    20041 cgaccttttg gaacaccaca ggcacacgca tcgaacggct gaatcaaaga ttcagaccga
    20101 tggcagtccg cacccgagtt tatgcggcaa acagcgaggc tacggcaacc cgccccctct
    20161 ccctgtggga gagggttagg gagagggcgg taagccgcag gcttacatca aagccgataa
    20221 cgctttcgtt acaactccgc ccactgaacg aagccaaaac gacaaaccgc atcgtaaacc
    20281 acccaaccca taggagaacc ccatgcaaaa cgaaaccatc aacctgaaac agcaccttgc
    20341 cgccatcaaa gaatactggc agcccgaaat catcaaccgc cacgggttcc aattccgttt
    20401 ggtcaaactt ttgggcgatt acggctggca tacgcacgga tacagcgaca aagtgctgtt
    20461 tgccgtggag ggcgacatgg cggtggactt cgccgacggc ggcagcatga cgatacgcga
    20521 gggcgagatg gcggtcgtgc cgaagtcggt gtcgcaccgc ccgcgttcgg aaaacggctg
    20581 ctcggtggtg ctgattgagt tgtccgaccc gtccgaggcc gtctgaaaac gaagtttccg
    20641 aaggaagctg agtttctgcg aagctaaaag cagcctgcac cttcaatcaa tatgccgaaa
    20701 atacaacccc accgcacacc aacacacaaa ggaaatccca tgacacgctt caaatattcc
    20761 ctgctgtttg ccgccctgtt gcccgtgtac gcgcaggccg atgtttctgt ttcagacgac
    20821 cccaaaccgc agaaaagcac tgaattgccg accatcaccg ttaccgccga ccgcaccgcg
    20881 agttccaacg acggctacac tgtttccggc acgcacaccc cgctcgggct gcccatgacc
    20941 ctgcgcgaaa tcccgcagag cgtcagcgtc atcacatcgc aacaaatgcg caaccaaaac
    21001 atcaaaacgc tcgaccgcgc cctgttgcag gcgaccggca ccagccgcca gatttacggc
    21061 tccgaccgcg cgggctacaa ctacctgttc gcgcgcggca gccgcatcgc caactaccaa
    21121 atcaacggca tccccgttgc cgacgcgctg gccgatacgg gcaatgccaa caccgccgcc
    21181 tatgagcgcg tagaagtcgt gcgcggcgtg gcggggctgc tggacggcac gggcgagcct
    21241 tccgccaccg tcaatctggt gcgcaaacgc ccgacccgta agccattgtt tgaagtccgc
    21301 gccgaagcgg gcaaccgcaa acatttcggg ctgggcgcgg acgtatcggg cagcctgaac
    21361 accgaaggca cgctgcgcgg ccgcctggtt tccaccttcg gacgcggcga ctcgtggcgg
    21421 cggcgcgaac gcagccgcga tgccgaactc tacggcattt tggaatacga catcgcaccg
    21481 caaacccgcg tccacgcagg catggactac cagcaggcga aagaaaccgc cgacgcgccg
    21541 ctcagctacg ccgtgtacga cagccaaggt tatgccaccg ccttcggccc gaaagacaac
    21601 cccgccacaa attgggcgaa cagccgccac cgtgcgctca acctgttcgc cggcatcgaa
    21661 caccgcttca accaagactg gaaactcaaa gccgaatacg actacacccg cagccgcttc
    21721 cgccagccct acggcgtagc aggcgtgctt tccatcgacc acaacaccgc cgccaccgac
    21781 ctgattcctg gttattggca cgccgacccg cgcacccaca gcgccagcgt gtcattgatc
    21841 ggcaaatacc gcctgttcgg ccgcgaacac gatttaatcg cgggtatcaa cggttacaaa
    21901 tacgccagca acaaatacgg cgaacgcagc atcatcccca acgccattcc caacgcctac
    21961 gaattttccc gcacgggtgc ctacccgcag cctgcatcgt ttgcccaaac catcccgcaa
    22021 tacggcacca ggcggcaaat cggcggctat ctcgccaccc gtttccgcgc cgccgacaac
    22081 ctttcgctga tactcggcgg cagatacagc cgttaccgca ccggcagcta cgacagccgc
    22141 acacaaggca tgacctatgt gtccgccaac cgtttcaccc cctacacagg catcgtgttc
    22201 gacctgaccg gcaacctgtc tctttacggc tcgtacagca gcctgttcgt cccgcaatcg
    22261 caaaaagacg aacacggcag ctacctgaaa cccgtaaccg gcaacaatct ggaagccggc
    22321 atcaaaggcg aatggcttga aggccgtctg aacgcatccg ccgccgtgta ccgcgcccgt
    22381 aaaaacaacc tcgccaccgc agcaggacgc gacccgagcg gcaacaccta ctaccgcgcc
    22441 gccaaccaag ccaaaaccca cggctgggaa atcgaagtcg gcggccgcat cacgcccgaa
    22501 tggcagatac aggcaggtta cagccaaagc aaaacccgcg accaagacgg cagccgcctg
    22561 aaccccgaca gcgtacccga acgcagcttc aaactcttca ctgcctacca ctttgccccc
    22621 gaagccccca gcggctggac catcggcgca ggcgtgcgct ggcagagcga aacccacacc
    22681 gaccctgcca cgctccgcat ccccaacccc gccgccaaag cccgcgccgc cgacaacagc
    22741 cgccaaaaag cctacgccgt cgccgacatc atggcgcgtt accgcttcaa tccgcgcgcc
    22801 gaactgtcgc tgaacgtgga caatctgttc aacaaacact accgcaccca gcccgaccgc
    22861 cacagctacg gcgcactgcg gacagtgaac gcggcgttta cctatcggtt taaataaggt
    22921 cgtctgaaaa cggagtttct gcgaagctat agtggattaa caaaaaccag tacgg
//
LOCUS       DAXHTY010000023        22557 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXHTY010000023 DAXHTY010000000
VERSION     DAXHTY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22557
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.023"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(100..528)
                     /locus_tag="ACFNMK_000760"
     CDS             complement(100..528)
                     /locus_tag="ACFNMK_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC6364072.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAESPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(593..2341)
                     /locus_tag="ACFNMK_000761"
     CDS             complement(593..2341)
                     /locus_tag="ACFNMK_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC6364073.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPPAGDFGARFKQNTDHIVLTHVFNSGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2668..4101
                     /gene="ccoN"
                     /locus_tag="ACFNMK_000762"
     CDS             2668..4101
                     /gene="ccoN"
                     /locus_tag="ACFNMK_000762"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC6364074.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4128..4739
                     /gene="ccoO"
                     /locus_tag="ACFNMK_000763"
     CDS             4128..4739
                     /gene="ccoO"
                     /locus_tag="ACFNMK_000763"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC6364075.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4744..4914
                     /locus_tag="ACFNMK_000764"
     CDS             4744..4914
                     /locus_tag="ACFNMK_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC6364076.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4938..5849
                     /gene="ccoP"
                     /locus_tag="ACFNMK_000765"
                     /pseudo
     CDS             4938..5849
                     /gene="ccoP"
                     /locus_tag="ACFNMK_000765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            5925..6269
                     /locus_tag="ACFNMK_000766"
                     /pseudo
     CDS             5925..6269
                     /locus_tag="ACFNMK_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
     gene            6471..7880
                     /locus_tag="ACFNMK_000767"
     CDS             6471..7880
                     /locus_tag="ACFNMK_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC6364077.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7981..11190
                     /gene="recC"
                     /locus_tag="ACFNMK_000768"
     CDS             7981..11190
                     /gene="recC"
                     /locus_tag="ACFNMK_000768"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC6364078.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELRPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPHIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGAGAA"
     gene            complement(11260..12828)
                     /gene="mtrF"
                     /locus_tag="ACFNMK_000769"
     CDS             complement(11260..12828)
                     /gene="mtrF"
                     /locus_tag="ACFNMK_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC6364079.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRTVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13162..13374
                     /locus_tag="ACFNMK_000770"
     CDS             13162..13374
                     /locus_tag="ACFNMK_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364080.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENIHAMIGEIKNAI"
     gene            complement(13653..14285)
                     /gene="mtrR"
                     /locus_tag="ACFNMK_000771"
     CDS             complement(13653..14285)
                     /gene="mtrR"
                     /locus_tag="ACFNMK_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC6364081.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDTEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
     gene            14693..15931
                     /gene="mtrC"
                     /locus_tag="ACFNMK_000772"
     CDS             14693..15931
                     /gene="mtrC"
                     /locus_tag="ACFNMK_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC6364082.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGTKDTVMIVNAQ
                     GGMEPREVTVAQQQGANWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWMPSESQA
                     SAPQSGVQTASEAKPASEAE"
     gene            15943..19146
                     /gene="mtrD"
                     /locus_tag="ACFNMK_000773"
     CDS             15943..19146
                     /gene="mtrD"
                     /locus_tag="ACFNMK_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC6364083.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTNENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            19201..20604
                     /gene="mtrE"
                     /locus_tag="ACFNMK_000774"
     CDS             19201..20604
                     /gene="mtrE"
                     /locus_tag="ACFNMK_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC6364084.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTRSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYEAAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(20806..21003)
                     /locus_tag="ACFNMK_000775"
                     /pseudo
     CDS             complement(20806..21003)
                     /locus_tag="ACFNMK_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21003..21209)
                     /locus_tag="ACFNMK_000776"
                     /pseudo
     CDS             complement(21003..21209)
                     /locus_tag="ACFNMK_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            21712..22491
                     /locus_tag="ACFNMK_000777"
     CDS             21712..22491
                     /locus_tag="ACFNMK_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC6364085.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT         5487 a         5509 c         6107 g         5454 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaagacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgttttc ggcttgtaat cagacgcggg cgttgtccag
      121 ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga tgatttcgtc
      181 aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc gcgtaccggt
      241 ccggcaatag gcaaggacgg gagattcggc ttgtccgatg agttggcgga aggtttcgac
      301 atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag taacgcctgc
      361 ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt cgcggtcggg
      421 gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat cggctttggt
      481 cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt tgtttccttt
      541 actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa gtttaaccga
      601 acaaccagtt gtccaacagg ttccggtctg tgatatgtag gatggcagta tccgctgccg
      661 ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag tggatattga
      721 tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag gcataaccgt
      781 ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg ccgctgttga
      841 agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca aaatcgcctg
      901 ccggcgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg ggaagtggca
      961 ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa tcttcggtac
     1021 tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc tgacagcgga
     1081 tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga tagagtttgt
     1141 ccattaacgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc agatcaaggc
     1201 acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg ctgttttcat
     1261 ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc tgcctcaaac
     1321 ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa taagattcgg
     1381 gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta ataccttcga
     1441 atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg aacgcggcag
     1501 gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt ccgagcaatg
     1561 tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg cggtcggcga
     1621 gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg acgtggagca
     1681 ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct gtttcgcaga
     1741 tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg ccgcttaagg
     1801 caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag cccatctcga
     1861 caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc cctgtttccg
     1921 gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag cggtgcggca
     1981 gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa ccgcgttctg
     2041 tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag cggatttgcc
     2101 actcgccgcg tacggcggca gcgtgccagc ggttttttgc aatttgttcg agcggcatgg
     2161 acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc cgaatcagat
     2221 agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca tcttgtgtga
     2281 atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta taatgaatca
     2341 taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg cagcttaacc
     2401 tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg cggtcgggtt
     2461 gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc aaagggctcg
     2521 ccggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa atttagaaaa
     2581 aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga taaaaatctt
     2641 tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta caaagtggtg
     2701 cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt cggcgttatc
     2761 gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc ttggttccac
     2821 ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg cggttgcggc
     2881 ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct tttcggcggt
     2941 tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc cgtcgtcagc
     3001 ttccctatgg gttggactca aggtaaggaa tatgccgaac tggaatggcc gatcgatatt
     3061 ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat tgccaaacgt
     3121 aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt ggcggtcgca
     3181 cttttacaca tcgtcaacaa tatcagcatc ccggcagggt tgatgaagtc ataccccgtc
     3241 tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataacgc cgtgggcttc
     3301 ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca agcagcccgc
     3361 cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt cacctatatg
     3421 tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca atctttgggt
     3481 atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa cggcatcatg
     3541 accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt cctgattgta
     3601 tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat taaaacggtc
     3661 aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg tgcgttgggc
     3721 tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct gttcggcaaa
     3781 gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac catcggcgtg
     3841 gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat gtggagttct
     3901 ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg caccatgcct
     3961 tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg cattatggcg
     4021 tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat ccctgcggtt
     4081 tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg aaattacaac
     4141 aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta gtcagtgtcg
     4201 gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag ccggcaccgg
     4261 gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc cgtgagggct
     4321 gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag cgttacggtc
     4381 attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt tccaaacgta
     4441 ccggtcctga tttggcacgt gtaggcggcc gctattccga cgaatggcac cgtatccacc
     4501 tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca tggcttgcac
     4561 gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt aaagtaggta
     4621 ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac aaatccgagc
     4681 tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac gtaaggtaac
     4741 atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat ctgtttcctg
     4801 ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa tgccgcaaac
     4861 agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg ttaatattgt
     4921 gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc tggaatatat
     4981 atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg ctgtctcaaa
     5041 atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat gagtgggacg
     5101 gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt gttttgacgt
     5161 ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac aaaggtctgc
     5221 tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat gagcaatacg
     5281 gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa gaccctcaag
     5341 ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac ggctctgatg
     5401 ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg ggcggtgatc
     5461 ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct gcctggggtc
     5521 ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc ctttcaaaac
     5581 ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc agcggtccgc
     5641 ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt ttgggtccga
     5701 acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc gaaaccatta
     5761 ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa gacaaactgc
     5821 atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct ccggtgaaaa
     5881 aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct gctcctgcag
     5941 aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa gccgaggaaa
     6001 aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt tgtgccgcct
     6061 gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat tgggccgacc
     6121 gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc aatacgatgc
     6181 ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg gttgactata
     6241 tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt atcggttatg
     6301 aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc ggttttaaac
     6361 gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt agtctttatt
     6421 gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta atggatacac
     6481 aaatcaaaac tgaagccgac aatcagagca accggcgtta tctgaccgtt tggcggtggc
     6541 atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt acgggtttgg
     6601 gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat gttgtgccgc
     6661 aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc gttaatccgg
     6721 aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt gccgtgttcc
     6781 gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg gcaaaagtgg
     6841 tcaacaccat gccgcgcaat cagggttggt atcacacgat ggatgaaatc cacagcgata
     6901 tgatgctcgg ttcgacaggc gattatcttt tggaaacggc tgcatcgctg acgattatca
     6961 tgataatcag cggtttgtac ctttggtggg tgaaacggcg cggcatcaag gcgatgctgc
     7021 tgccgtcaaa aggcagggcg cgttcttggt ggcggaatct gcacggcacg tttggaactt
     7081 gggtgtcgtt gattttgctg ttgttctgcc tgtcgggtat tgcttgggcg ggtatttggg
     7141 gcggcaagtt cgtacaggct tggagtcagt tccctgccgg taaatggggt gtcgaaccga
     7201 accccgtttc agtcgtgccg acccacggcg aggtattgaa tgacggcaag gttaaggaag
     7261 tgccgtgggt tttggagctt acgcctatgc ctgtttcagg gacgactgtg ggcaaagacg
     7321 gcattaaccc tgacgagccg atgacattgg aaaccgtcga ccgctttgcg cgggaaatcg
     7381 gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta tggactttgt
     7441 cgcaggattc tatgagttac gacatgatca gcccgtttgc cgaccgcacg gtacatatcg
     7501 accagtacag cggcaaaatc cttgccgaca tccgttttga cgattacaac ccgttcggca
     7561 aatttatggc ggcaagcatt gcgctgcata tggggactct gggctggtgg agcgtgttgg
     7621 cgaacgtctt gttctgcctt gccgtcattt ttatcggtat cagcggctgc gtgatgtggt
     7681 ggaaacgccg tccgaccgga gcggtgggca tcgttccgcc ggcgcagaaa gtcaagctgc
     7741 cggtttggtg gatgatggca ttgccgctat tggcaatcgc actgctcttc ccgaccgcac
     7801 tgcttgccat tgccgtgatt tggctgttgg atacgctgct gttgtcgcgg attcctgttt
     7861 tgaggagatg gtttaaatga ccaaatgccg tctgaaaggt tcagacggca ttttgtttga
     7921 aggcggacgg gggaaaggct atataatccc gaatactttt ccgcaacttt tgtcgaaatc
     7981 atgttttatc tgtatcaatc caaccgtctt gaaacgctgg cggcattgtt tgcccgcatt
     8041 caaaaagtca aaccgctgaa atcggcttta cagcccgaac agattattgt gcagagtcag
     8101 gggatgcgcc gctatctcaa tacctgcctc gcccgcgatt tgggcgtggc ggcgaatttg
     8161 tcgttcagcc tgcccgccgg cctgacgtgg aagctgatga aaaaactgat tcccggtatt
     8221 ccggaactcc gcccgtttgc acccgaagtc atgcgctggc ggctgctgga tttgttccgc
     8281 agtgaagcat tccggaatac ggcagaattt gaagatgtga ggaatgtgct gcaagactat
     8341 ctgggcagcg gcgaatcggc agattaccag cttgcgggac agcttgcgga catattcgac
     8401 caatacctcg tgtaccgtcc tcagtggata gacgcttggc agcagggcag gcggctcggt
     8461 ttgggcgacg acgaaatctg gcagtccaaa ctgtggcgtt acctcgacga tggcaggcag
     8521 agcgcgccgc accgtgtcgc gttgtgggaa aagctgcttg ccgcgttgga caaggataag
     8581 ctgcccgagc gttatttcgt gttcggcatt tccacgatgg cgccgatgta tcttcagctt
     8641 ttgcacaagc tgtccgaaca ttgcgatgtg ttcgtgttcg cactcaatcc gagcgggatg
     8701 tattggggca acgtcatcga agcggcgcaa atcctcaaag gcggcggcga tcccgattta
     8761 actcaggcag ggcatccgct gctcgcctca ttgggcaagc aggggcgcga ctttttcgac
     8821 tttttgaacg aaatggaaat agaaggagaa acgccggtat ttgaagaagg cgggcgcgat
     8881 acgcttttgc acgccctgca aaccgatatt caaaacctga aaatgccgtc tgaaaatgtg
     8941 ggaagcgtca acacgggcga cggctcgata cgcatcgtct ccgcacacag ccccttgcgc
     9001 gaattgcaga tactcaaaga caagctgttg aaaattctgc atgaacatcc cgattggcag
     9061 ccgcatgata tcgccgtatt aaccccgcac atcgaatcct atacgccttt tatcgaagcc
     9121 gtgttcggac aggcgcagcc cggtgcgcag gcattgccgt attccgtttc agacgtgaaa
     9181 atcagccgcc gacaaccgct gtttcatgct ttgtcatgcc tgttcgactt gttggaaagc
     9241 cgatttgaag tcgataaagt gcttgtgctt ttagaaaccg cacccgtgtt gcgccgtttc
     9301 ggactgactg aggacgattt gccgcttttg cacgatatgg ttgccgattt gaacgttcac
     9361 tggggtttgg acggagaaat gcgcggcggc acggatcagc tgtttacctg gaagcaggcg
     9421 gtagaacgca tgatattggg ctggatgctg cccgaaggcg gcaacccgat gtggcaggat
     9481 gtcagcgcgt ggtatgccga cgtgaaccaa accgccctgt tcggacgttt tgccgccctc
     9541 ctcgaaaccc tgtcggatat tgtacggata tggcggcagc ccgcaacgat cggcgaatgg
     9601 gtggcgcgtt gccgggattt gcttgaaaca ttgttccaag ctgaagccga tgaccaaaag
     9661 tcagtccaaa accttgaaaa cgaatgggtc aaatggcagg cggaaacaga attggcgcaa
     9721 ttttccggac agttgccgca acaaaccgtc atccgccata tccgacgttt cctcgacagc
     9781 gaaagcgagg caggcttttt acgcggcggc atcacctttt gcagtatggt gccgatgcgg
     9841 agcctgccgt tcaaagtcat ctgcctgttg ggtttgaacg acggagattt tccccgcaat
     9901 accaaagccg ccgtattcga cctgattgcc aaacatcccg ccaaaggcga ccgcgcccgc
     9961 cgcgatgacg accgctacct cttcctcgaa gccctcatca gcgcgcgcga aatcctctac
    10021 ctgtcctaca tcgggcgcga catccgcaaa gacgaagagc ttgctccgtc ttcgctgttg
    10081 ggcgaactca tcgacacagt tgccgctatg gcgggcatcg gtagccgcca acttgcacaa
    10141 aactggatag aacagcatcc gctgcaagcc ttctcgcgcc gatattttca agaaggcgga
    10201 cgttcagacg gcatattcgg cacgcgtacc gactacgcca ctgcgctcgg acaaacgccc
    10261 gaaccgccgc aacccttttt cgaccaaccc gtagaaaacg ccgaacctgt tgccgaaatc
    10321 ggacaggacg aatttatccg tttctggcgc aaccccgtca aagtatggct tcagcagcag
    10381 cttgcgtgga gcgaacccca tatcggcgaa gcctgggagc ctgccgaacc cttcgagccg
    10441 caacacgccg atcaaatcgc cgaaacctac atcgaagcac ggcgcgaagg acgggatttt
    10501 gcccaaaccg ccgcccgcat cggggcggaa agcctcctgc cgtcgggaga gttgggcaga
    10561 ctttggcagc agaacttcca aactgctgcc aaacaaatcg acacggcggt tttaaacagc
    10621 cccaaactgc caccgctttc atatgccata ccgtcggacg ggcaaatcct gaaaggcagc
    10681 ttgggcaatc tgtaccgctg cggacaagtg ttttacgcct acggcaaacc caacgcgccg
    10741 caacgtatcg cttttctgct ggaacacctg atattttgcg ccgttatgcc gtctgaagcc
    10801 gaaacgcggc aaacctttat cgtccaatcc ggagaaaccg aaatattggc ggaaatcgcg
    10861 caagacaggg cattgcagct attgtcggaa tggatggcgt ttttcaatat cgggcaaaac
    10921 cgcccgctgc cgttttttgc caagacctcg cttgccgctg ccgaagcgtt tgcccaaaaa
    10981 caagattggg aagccgccct gaaaaaagcc caaaccgcct attacggcaa caaagtcagc
    11041 aaagggcaga aagactatac cgaagtcgcc ctcgtgttcg gcaacgcaag ccaaaaccca
    11101 ctcgaacaac ccctgtttga aaacctcgcc cgcctgcttg ccgacacgct tgccgcagcg
    11161 gaaaaaaggg aaggggccgg agcagcctga cgggatggaa aggcaaatgc cgtctgaaaa
    11221 cagcaaaaag gttttcagac ggcattttgt ttatcgtgtt taaggtgcgg gatagaatgt
    11281 gggcgcgccg ggaccgacgg gcaggcccaa aacaaatacc caaatgcaga ataaggcaat
    11341 ccacgcaatc aagaagaaag cggaatacgg caacatcata gaaatcagcg tacccacgcc
    11401 cgcatctttt ttgtatttga tcaccgtcgc cataatcagc ccgaaataac tcatcatcgg
    11461 cgtaataata ttggtaacgg aatcaccgat gcggtaagcg gcttgaatga cttcgggcgc
    11521 gtagccggcc aacatcagca tagggacgaa aatcggcgca gttaccgccc attgcgcgga
    11581 ggcggagcct atcatcagat tgataaaagc acaaattaaa ataaaaccga taaacaacac
    11641 gctgccgccc aagccgactt cttttaagaa cgtcgcccct ttaacggcaa tatattgccc
    11701 aatattcgtc caattaaaaa atgcgacaaa ctgtgcggca aaaaagatga tgaccaaata
    11761 aagccccaga gtactcatcg attcggccat cgcattaacg acttcctgtt cgccgcgcaa
    11821 acttcgggtt acccggccat aaacaatgcc cggcagtgca aacaacaaga aaataaaaac
    11881 aacaattgat tttaaaaacg gcgaaccgga aaccaatcct gtttcaggat gacgcaaaat
    11941 accgtcggca gggacgatgc tccaagccaa tagggcggat aaggcaacaa acaccacgcc
    12001 agcccaaatt aatcctttat attccaaagg cgtgatttca ttggaatgtc gaatgtcttt
    12061 ttcttcttgt gacaaatctg attgataagg gcccaattgc ggttcgacga ttttttcagt
    12121 aacaaaataa ccaatcaaag caatcacaaa cgtactggct accataaaaa accagttggc
    12181 ttcagggcct acgacgtagt cgggatggat gatttgcgcc gcctgttggg tgatgcctgc
    12241 caagagcgga tcgattgtgc ctaagaacag attggccgaa taaccgcccg aaacgccggc
    12301 gaaagccgca gccagaccgg caagcggatg gcggccgagg gaatgaaaga tgatggcgga
    12361 caaagggatt aggacgacat agcccaattc agaagcggta ttagataaaa tccctgtaaa
    12421 aacaaccata aaagtagtga gtttgcgtgg agattttgtg agcaataagc gcattaatgc
    12481 ggaaatcaag cccgattttt ccgcaatccc cacgcccaat aaagaaacca acaccgttcc
    12541 caacggcgcg aaaccggtga aatttttaac ggtatgcgtc aggattttga tcaaaccgtc
    12601 agcatcgagc aggctgacaa cgtgaatcaa accgtcatcg gcacgtcctt tcgcaccaac
    12661 agtgcgcgga tcggggacgg atagtccgaa atacgcaccg gcggcagagg caatcagcaa
    12721 taacacaatg aaaataataa aaagcgtaac cgggtgcggc aacatattgc ccagccattc
    12781 gactgtgcgt aaaaatcgtc cgtcccgttg cgtatcggtt tgactcatct gcttctcctt
    12841 aaaaaaaata tattgagtgc attaaatttt tgaatgtaac atgaagtgtt ttgtaagcaa
    12901 actaaaataa tcgtatatgt atataaccgt aatagattaa aacagaaaat gcccacatcg
    12961 ttattttcat aaaggcggta atccgaaaac ttgacgttaa agccttatca ggaatgactg
    13021 gaactcaaaa aaacggattc ccgcctgcgc gggaatgacg ggattttcgg tttttgtttg
    13081 caagaatgcc ggagaagaat gcagtttaca ttttgaatga tattgttatt aattgtaaat
    13141 aaatattgga aggtaaatga tatgaaaaat gaaattcaaa aaatcatgga caaatataat
    13201 ccatggcatg aagatgattt tgagtcgtat gaagacattg ccaaagatgt atcgctgatg
    13261 acagataaaa cgttcattga acattatttg ttagaagttt attcagaaga aaacggacat
    13321 tttgaccaag agaatatcca tgcgatgata ggggaaatta aaaatgcaat ttagtgaata
    13381 tgagaccttt gcaaaaatag tctgttaacg aaatttgacg cataaaaatg cgccaaaaaa
    13441 ttttcgattg cctaaaacct tcctaatatt gggcaaaaag taggaaaaat cagaaaagtt
    13501 ttgcattttg aaaatgagat tgagcataaa attttagtaa cctatgttat tgcaaaggtc
    13561 tcaatatgta tttttagaag aaggctttgt gtcattttga tgccgtctga aagggtttgt
    13621 tcgtttcaga cggcattgct accaaggctt gattatttcc ggcgcaggtc gggatggttt
    13681 tccaagttgt ccatcattat cccgatgatg cgcggggcgg ttttgcccaa atcgaaacgt
    13741 tcgcaggaag agaaccaccg ccaaatcagc ccgtccaagg ttgatttgat gaagataact
    13801 gccgtttcct tgtccaaatc gtcagccaaa tcctgatttt ccaccgcttc ggtcaaaacg
    13861 gcggtaattt tctcgcgcca gattgcctga tgcttgcggg caatggcgat aacggcggcg
    13921 ttttgctccg tgtgttcgca ttttaaaaac aggatgttgt ggaatttgta gtagatgtcg
    13981 ttgctttgca gccgctcgaa aaagtgcagc agcgtgtggc ggaataccgc ccaagaccct
    14041 ccttcggcat cttcggtatc ttgcgcgatg cagttttcga tgtcgtcgca gatacgttgg
    14101 aacagcgcgt cgaacaagtc ttccttattt ttgaaatgcc aatagagcgc gccgcgcgtt
    14161 acgccggcgg cttgggcgat ttcgttgagc gaggtgcgcg caatcccttt gcggtaaaag
    14221 gtttccaagg cggcaagcat caggtgttct ttggttttca aggcttcggt tttggttttt
    14281 ctcataatgg cgttttcgtt tcgggtcggt ttgatgaggg cggattataa aaaagacttt
    14341 gtaaccatgc aatcgtgtat gtataatgaa acccatgaaa ttgagactac atctcaactt
    14401 tgaaaacagg cataacctgc tttgcaaccc gttcgaacat tcgggtatca aagcaggcgg
    14461 tttttatatc ctgaaatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
    14521 acagtacaga cagatagtac ggaaccgatt cacttggtgc ttgagcacct tagagaatcg
    14581 ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca ctataagaat
    14641 gcctgttgat tggcaaaacc ctttacatca aaccagacag ataggataaa taatggcttt
    14701 ttatgctttt aaggcgatgc gtgcggccgc gttggctgcc gccgttgcat tggtactgtc
    14761 gtcttgcggt aaaggcggag acgcggcgca gggcgggcag cctgctggtc gggaagcccc
    14821 tgcgcccgtc gtcggtgtcg taaccgtcca tccgcaaacc gtcgcattga ccgtcgagtt
    14881 gccagggcgt ttggaatcgc tgcgtaccgc cgatgtccgc gcccaagtcg gcggcatcat
    14941 ccaaaaacgc ctgttccaag aaggcagtta tgtccgtgcc ggacagccgc tgtatcagat
    15001 cgacagttcc acttatgaag caggtctgga aagtgcgcgc gcgcaactgg caacggctca
    15061 ggcaacgctt gccaaagcgg atgcggattt ggcgcggtac aagcctttgg ttgccgccga
    15121 agccgtcagc cggcaggaat acgatgctgc ggtaacggcg aaacgttctg ccgaggcagg
    15181 tgtcaaagca gcacaggcgg cgatcaaatc tgccggcatc aatctgaacc gttcgcgcat
    15241 taccgcgccg atttccggct ttatcggtca gtccaaagtt tccgaaggta cgctgttgaa
    15301 tgcgggcgat acgaccgtgc tggcaaccat ccgccaaacc aatccgatgt atgtgaacgt
    15361 tacccagtct gcatccgaag tgatgaaatt gcgccgtcag atagccgaag gcaaactgct
    15421 ggcggcggat ggtgtgattg cggtcggcat caaatttgac gacggcacag tttaccctga
    15481 aaaaggccgc ctgctgtttg ccgatccgac cgtcaacgaa tcgaccggtc agattaccct
    15541 gcgcgccgcc gtaccgaacg atcagaatat cctgatgccc ggcctgtatg tgcgcgtgct
    15601 gatggaccaa gtggcggtgg ataatgcatt catcgttccg cagcaggcgg taacgcgcgg
    15661 tacgaaagat accgtgatga ttgtgaacgc ccaaggcggt atggaacccc gcgaggtaac
    15721 ggtcgcccaa cagcagggtg cgaattggat tgttacatca ggtctgaagg acggggacaa
    15781 ggtggttgtg gaaggcatca gtatcgccgg tataacgggt gcgaaaaagg taacgcctaa
    15841 agaatggatg ccgtctgaaa gccaagcctc tgcgcctcaa tccggcgttc agacggcatc
    15901 tgaagccaaa cctgcttctg aagcggaata aggaaggcat cgatggctaa attttttatc
    15961 gaccgcccca ttttcgcgtg ggttatttcg attttcatta ttgcggcggg tattttcggc
    16021 atcaaaagcc tgcctgtttc gcaatatccg tctgtcgccg ccccgaccat tactttgcac
    16081 gccacttatc cgggtgcgtc cgcgcaggta atggaagaca gcgtgctttc cgtgatcgag
    16141 cggaatatga acggcgtgga aggtttggat tatctgtcca cttccgccga ttcgagcggc
    16201 agcggcagcg tgagcctgac ctttacgccc gataccaacg agaatctggc gcaggtggaa
    16261 gtgcagaaca agctttccga agtattgagc acgctgccgg caaccgtcca gcaatacggc
    16321 gtaaccgtat ccaaggcgcg ttccaatttc ctgatgattg tgatgctttc gtcggatgtg
    16381 cagtcaaccg aagagatgaa cgactacgcg cagcgtaata tcgttcccga actgcagcgt
    16441 atcgaaggcg tggggcaggt acgcctgttc ggcgcgcaac gcgcgatgcg gatttgggtt
    16501 gatcctaaga aactgcaaaa ctacaatttg tcgtttgccg atatcggcag cgcgctgtcc
    16561 gcccagaacg tccagatttc agcgggttct atcggttcgc ttcccgccgt tcgcggacag
    16621 acggttacgg ctaccgtaac ggcgcaaggg cagttgggta cggcagaaga attcggcaac
    16681 gtcatcctcc gcgccaatac cgacggttct aatgtttacc tgaaggatgt ggcaagggtc
    16741 ggactgggta tggaagacta ttcttcctca acccgtctga acggtgtaaa taccaccggt
    16801 atggcggtga tgctgtccaa cagcggcaat gcgatggcga cggcaaaggc ggttaaagaa
    16861 cgcatggcga cgttggaaaa atactttcct cagggtatga gctggaaaac cccttacgat
    16921 acttccaaat tcgtcgaaat ttcgattgaa aaagtgattc atactttaat cgaagcgatg
    16981 gtgctggtgt ttgtcgtaat gtatctcttc ctgcaaaaca tccgttatac gctgattccg
    17041 accatcgtcg taccgatttc gctgttgggc ggtttcgcct tcatctctta tatgggcatg
    17101 tcgattaacg tactgaccat gtttgcgatg gttttggtca tcggcatcgt ggtcgatgac
    17161 gcgattgtgg tggttgaaaa cgtcgagcgc attatggcgg gtgaaggctt gccgcccaaa
    17221 gaagcgacca aaaaagcgat gggtcagatt tcgggcgcgg tcatcggtat taccgccgtt
    17281 ctgatttccg tgttcgtacc gttggcgatg ttcagcgggg cgacgggcaa tatttacaaa
    17341 cagtttgccc tgacgatggc gtcatcaatc gcattctccg ccttccttgc gctgaccctt
    17401 acccctgctt tgtgtgccac aatgttgaag acaatcccga aagggcatca cgaagagaaa
    17461 aaaggtttct tcggctggtt taacaagaaa ttcaacagtt ggacgcacgg ttacgaaggc
    17521 cgggttgcca aagtgctgcg taagactttc cgcatgatgg ttgtctatat cggcttggcg
    17581 gttgtgggcg tgttcctgtt tatgcgcctg ccgacttcat tcctgccgac cgaagaccaa
    17641 ggcttcgtca tggtcagcgt gcaactgcct gcaggagcga cccaagagcg caccaatgcg
    17701 actttggcgc aagttaccca actggcgaaa agcattcctg aaatagaaaa catcattacc
    17761 gtttccggct tcagcttttc gggcagcggt cagaatatgg cgatgggttt tgccatattg
    17821 aaagattgga acgagcgtac cgcgcccggc agcgatgccg ttgcgattgc cggcaagctg
    17881 acgggtatga tgatggggac gcttaaagac ggttttggca tcgccgtcgt cccgcctccg
    17941 attctggagt tgggcaatgg ttcgggcctg agcatcaacc tgcaagaccg caataatacc
    18001 ggccataccg cattgctggc gaagcgcaac gagttgattc agaaaatgcg tgccagcggt
    18061 ttgtttgacc ccagcaccgt ccgtgccggc ggtttggaag actcgccgca gttgaaaatc
    18121 gacatcaacc gtgccgccgc ggcggcgcaa ggcatttcgt ttgccgacat ccgcaccgca
    18181 ttggcaagtg cgctgagttc gtcttatgtc agcgacttcc cgaaccaagg ccgtctgcaa
    18241 cgcgtgatgg tgcaggccga cgaggatgcc cgtatgcagc ctgccgatat tttgaacctg
    18301 accgtgccga acaaatccgg cgtcgccgta ccgctttcca ccattgctac tgtttcttgg
    18361 gaaaacggta cggaacagag cgtacgcttc aacggttatc cttcgatgaa gctgtccgct
    18421 tcgcctgcaa ccggcgtttc caccgggcag gctatggcgg cggttcaaaa aatggttgac
    18481 gaattgggcg gcggttacag cctggagtgg ggcggacagt cgcgcgaaga ggcaaaaggc
    18541 ggctcgcaaa ccctgatttt gtacggtttg gcggttgcag ccgtattttt ggtacttgcc
    18601 gcgctttatg aaagctggtc gattccgctg gcggtcatcc ttgtgattcc gttgggtttg
    18661 atcggtgcgg ctgcgggcgt aaccgggcgc aacctgtttg aaggactgtt gggcagcgtt
    18721 ccctcgttcg ccaacgacat ctactttcaa gtcggtttcg ttaccgtgat gggtttgagt
    18781 gcgaaaaatg cgattttgat tatcgaattt gccaaagacc ttcaagcgca agggaaaagc
    18841 gcggttgaag ccgctttgga agccgcccgc ctgcgtttcc gtccgattat catgacctcg
    18901 ttcgccttta ttttgggcgt ggttcccctg tatattgccg gcggtgcaag ttctgccagc
    18961 cagcgcgcca tcggtacaac cgtattctgg gggatgctca tcggcacgct cttgtccgtg
    19021 ttccttgttc cgcttttcta tgtggtggtg cgcaaattct tcaaagaaac cgcgcacgaa
    19081 cacgaaatgg cagtaaaaca cgctgccgaa gcgggcatca ccggttcgga cgacagccaa
    19141 cattaagcaa ccatgccgtc tgaacgccca cgggttttca gacggcatca ggactttttt
    19201 atggatacta cattgaaaac caccttgact tctgttgcag cagccttcgc attatccgcc
    19261 tgcaccatga ttccccaata cgagcagccc aaagtcgaag ttgccgaaac gttcaaaaac
    19321 gataccgccg acagcggcat ccgcgccgtc gatttgggtt ggcacgacta ttttgccgac
    19381 ccgcgcctgc aaaagctgat cgacatcgca ctcgagcgca ataccagttt gcgtaccgcc
    19441 gtattgaaca gcgaaatcta ccgcaaacaa tacatgattg agcgcaacaa cctcctgccc
    19501 acgcttgccg ccaatgcgaa cgactcgcgc caaggcagct tgagcggcgg caatgtaagc
    19561 agcagctaca aagtcggact gggtgcggca tcttacgaac tcgatctgtt cgggcgtgta
    19621 cgcagcagca gcgaggcggc actgcaaggc tatttcgcca gcaccgccaa ccgcgatgcg
    19681 gcacatttga gcctgattgc caccgttgcc aaagcctatt tcaacgaacg ttacgccgaa
    19741 gaagcgatgt ctttggcgca acgtgttttg aaaacgcgcg aggaaaccta caagctgtcc
    19801 gaattacgtt acaaggcagg cgtgatttcc gccgtcgccc tacgtcagca ggaagcccta
    19861 atcgaatctg ccaaagccga ttatgcccat gccgcgcgca gccgcgaaca ggcgcgcaat
    19921 gccttggcaa ccttgattaa ccaaccgata cccgacgacc tgcccgccgg tttgccgctg
    19981 gacaagcagt tttttgttga aaaactgccg gccggtttga gttccgaagt attgctcgac
    20041 cgtcccgata tccgtgctgc cgaacacgcg ctcaaacagg caaacgccaa tatcggtgcg
    20101 gcacgcgccg cctttttccc atccatccgc ctgaccggaa gcgtcggtac gcgttctgcc
    20161 gaattgggcg ggctgttcaa aagcggcacg ggcgtttggt cgtttgcgcc gtctattacc
    20221 ctgccgattt ttacctgggg aacgaacaag gcgaaccttg atgtggcgaa actgcgccaa
    20281 caggcacaaa tcgttgccta tgaagccgcc gtccaatccg catttcaaga cgtggcaaac
    20341 gcattggcgg cgcgcgagca gctggataaa gcctatgacg ctttaagcaa acaaagccgc
    20401 gcctctaaag aagcgttgcg tttggtcggt ctgcgttaca aacacggcgt atccggcgcg
    20461 cttgatttgc tcgatgcgga acgcagcagc tattcggcgg aaggtgcggc tttgtcggca
    20521 caactgaccc gcgccgaaaa ccttgccgat ttgtacaagg cactcggcgg cggattgaaa
    20581 cgggataccc aaaccgacaa ataaccggtc gggcaataaa atgccgtctg aaacgataag
    20641 gtttcagacg gcatttcttt ctttatagtg gattaacaaa aaccagtacg gcgttgcctc
    20701 gccttagctc aaagagaacg attctctaag gtgctgaagc accaagtgaa tcggttctgt
    20761 actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatatg
    20821 tttgacggtt gcggtcaggc tgcgcggcgt gccgtaaacg tgaatgtcgg gcatggtgcg
    20881 gtagcgtttg ttgaacacgt tttcaaagtc caaaccgatg cgtgtggatt ttccgatttt
    20941 gtaatgcgcg gtcaaatcca gcgtggcgta ggggcgttgg gtcagtgcgg ctgcggcggc
    21001 agggaagaaa ccgccatact cgccggcatc ccgaagccga atacggcaac ggcaagcgca
    21061 atcagcagcg tataaagccc cgcctgatag cctttcagct tcaggacggt cagcgcggca
    21121 aagaaaaaga cgatgggtaa gagtgcggcg gcggcagtca gatacaggct gccgccgatt
    21181 gccgtgtagt tttgaaccca agtttccata attgaacatc tccgaaaata tttttctaat
    21241 cgtcggcaat agtggtcaaa ccaattaaag caacgttgca ttactttgcg aaactttaat
    21301 atttaggtca atatattttt gggcggttcg gcagatttga atcggagctt tcgtttaaat
    21361 cctgtcaaaa caaatatttg catgaacaaa aattgtagtt tggtgtagtt ttttcttgtg
    21421 tttcgggggc ggtgcgaggt aaatgccgtt gacggcggga acggcgtgtc attagaatcc
    21481 gccctgattg gtcagtccaa tttgatgttt gatgtatagt ggatgaacaa aaaccagtac
    21541 ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctgaag caccaagtga
    21601 atcggttctg tactatttgt actgtctgcg gcttcgtcac cttgtcctga tttttgttaa
    21661 tccactatat ttcggaaggg ttctgcaggg aatctttata aaggaggagt gatgaaactg
    21721 gtaaggcctc agaaaatcag cgatcaggta ttgtcggtat tggaggagcg cattgcggaa
    21781 ggggtttacg cggaaggggg caagattccg cccgaacgcg ttttggcgga agagttcggc
    21841 gtttcgcgcc cgtcggtcag gtcggcattg aatgttttgg tcgcccgtca ggttttggaa
    21901 gcgaggcagg gagacggtta ttatgtttcg gtcaagccgc agcaggattt tttgcaaagt
    21961 tggcaggagc ttttgggcaa gcattccaat tgggaacagg atgtttttga tttcagttgc
    22021 cacatcgagg gctgtatggc ggcattggcg gcagaacgcc gcacggatgc cgatttgaag
    22081 cggattgggt tttggcttga aaagtttgag gaggcgtgcg aaagcggcaa tttggaacat
    22141 cagagcgaag ctgatgtcag ctttcatcag acgattgccg atgcggcgca caatttgttg
    22201 ttcagccatt tgtcgggcgg tttgttgaaa atgctgtacc ggcagacgcg cagcagcctt
    22261 atttatctga atcaggaaga agatccgcgt ccgaagctga tggcgcagca ccgtgtattg
    22321 tatgaggcaa tatcaaaccg ccggccgggc gaggcttcgg aggcggcaaa ggcacatttg
    22381 aattatgtgg cgagcagcat cttgaaagac agggaatatc agagccgtaa ccgccacgca
    22441 gatactttgg cgcaaaacga tttgaagcgc gtgcaggatt gggaggtatg aataccgttt
    22501 agacggtctg gcatattcgg atgtatcggt atagtgaact aaatttaaac cagtacg
//
LOCUS       DAXHTY010000024        22556 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXHTY010000024 DAXHTY010000000
VERSION     DAXHTY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.024"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            143..1540
                     /gene="aspA"
                     /locus_tag="ACFNMK_000778"
     CDS             143..1540
                     /gene="aspA"
                     /locus_tag="ACFNMK_000778"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC6364086.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAVLKNAFAAKAEKFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1612..2487)
                     /locus_tag="ACFNMK_000779"
     CDS             complement(1612..2487)
                     /locus_tag="ACFNMK_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC6364087.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHGGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFSGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2489..3073)
                     /locus_tag="ACFNMK_000780"
     CDS             complement(2489..3073)
                     /locus_tag="ACFNMK_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC6364088.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRNEERGRQEEAFRREQARRE
                     QFYREQMRREQAFRQAFERQASRSRHAYEPSGGGSGRNYVLAAYILFGLGAIMLFMPI
                     VGVIFAYMPIVGVILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIG
                     VLIIIATNVWYFYRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3096..3575)
                     /gene="ybaK"
                     /locus_tag="ACFNMK_000781"
     CDS             complement(3096..3575)
                     /gene="ybaK"
                     /locus_tag="ACFNMK_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC6364089.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIESATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3650..4012
                     /locus_tag="ACFNMK_000782"
     CDS             3650..4012
                     /locus_tag="ACFNMK_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6364090.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKKDAATA"
     gene            4047..4910
                     /locus_tag="ACFNMK_000783"
     CDS             4047..4910
                     /locus_tag="ACFNMK_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC6364091.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVDAEL
                     EASAEPLAAQEPSLVFTAECVSDGICTVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLAADGEEAGIVLDSVQDSENFTALAVIKFSAAQKTLSAPNGGSFKA
                     VHLFFKTENAE"
     gene            5003..5962
                     /gene="ttcA"
                     /locus_tag="ACFNMK_000784"
     CDS             5003..5962
                     /gene="ttcA"
                     /locus_tag="ACFNMK_000784"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC6364092.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(6056..8995)
                     /locus_tag="ACFNMK_000785"
     CDS             complement(6056..8995)
                     /locus_tag="ACFNMK_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFC6364093.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSGGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIH
                     FNTMTYRELLEKLAQTAKIRMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTDKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9008..10699)
                     /locus_tag="ACFNMK_000786"
     CDS             complement(9008..10699)
                     /locus_tag="ACFNMK_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFC6364094.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELARLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYVARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKKIVINDEL
                     YWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSYGKTIIGK
                     LRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPLDLLKRLV
                     LSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARK
                     AGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDL
                     PLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGAYTAYLCEKRLYLMNAGFTSAD
                     LLAFIRKLDDDAAFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
     gene            complement(11057..11614)
                     /locus_tag="ACFNMK_000787"
     CDS             complement(11057..11614)
                     /locus_tag="ACFNMK_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6364095.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIDGFVIQGGGFTEDLVQKASDKAVANESGNGLKN
                     TVGTIAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMDAVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
     gene            complement(11679..12605)
                     /locus_tag="ACFNMK_000788"
     CDS             complement(11679..12605)
                     /locus_tag="ACFNMK_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6364096.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTSGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            complement(12593..13069)
                     /locus_tag="ACFNMK_000789"
     CDS             complement(12593..13069)
                     /locus_tag="ACFNMK_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC6364097.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            complement(13140..13595)
                     /locus_tag="ACFNMK_000790"
     CDS             complement(13140..13595)
                     /locus_tag="ACFNMK_000790"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC6364098.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            complement(13720..14835)
                     /locus_tag="ACFNMK_000791"
     CDS             complement(13720..14835)
                     /locus_tag="ACFNMK_000791"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC6364099.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     ACGQSLLNLASVSTENRYKIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            complement(14949..16046)
                     /locus_tag="ACFNMK_000792"
     CDS             complement(14949..16046)
                     /locus_tag="ACFNMK_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC6364100.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIGVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            complement(16128..17711)
                     /locus_tag="ACFNMK_000793"
     CDS             complement(16128..17711)
                     /locus_tag="ACFNMK_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HFC6364101.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     KHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEAFKTEDVAENLARRFPGLTF
                     DRMKINSQLLLQTILGRFSVSSDNAGKQFFEDHKTYVPARFTDYVAAFVEHGAEAFVQ
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            complement(17859..18071)
                     /locus_tag="ACFNMK_000794"
     CDS             complement(17859..18071)
                     /locus_tag="ACFNMK_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC6364102.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            18417..18572
                     /locus_tag="ACFNMK_000795"
                     /pseudo
     CDS             18417..18572
                     /locus_tag="ACFNMK_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            18701..20137
                     /locus_tag="ACFNMK_000796"
     CDS             18701..20137
                     /locus_tag="ACFNMK_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC6364103.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSAQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDRENTRRFWLGCGIAASLTVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFSWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFNGWGVLGSVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGTLHGTALVLLNTGDRYFGRDALCRLKYLAPLSWLITFHFVCLTFVVFNTASPD
                     DAGAVFTALFANANGWNAPQQADMLLLTSFASVMLLYPYLQRAFDGVVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            20148..21134
                     /gene="patB"
                     /locus_tag="ACFNMK_000797"
     CDS             20148..21134
                     /gene="patB"
                     /locus_tag="ACFNMK_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC6364104.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGEI
                     QEWKQNTDTAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYP
                     SFFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDR
                     ILEAAHTHYVQVVWLGIPYMKKAKLDGQMRYLDKLLSEYLKGKIILIPTAHTLSGGKD
                     RYTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            21131..22324
                     /locus_tag="ACFNMK_000798"
     CDS             21131..22324
                     /locus_tag="ACFNMK_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC6364105.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNASASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     IASKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILAYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRIA
                     RQGQTMFWSWQNAMGGVCSMKNWLNHGWAAKDGVHFSAKGYQRSAEMLADSLEELVRA
                     AAIRQ"
BASE COUNT         5259 a         6219 c         5474 g         5604 t
ORIGIN      
        1 acccgaaaac aacgtttccc gttttcagac ggcatacgat atggacaatt tcatccggcc
       61 ggtatagtgt tcatcctgcg gcggcatctt tccgatggcg cattttttca atattaatta
      121 tccgacttac aaaggtaaaa acatgactgt ccgtatcgaa cacgatttat tgggcgaccg
      181 cgagattccc gccgaagtgt attggggcat ccacactctg cgcgccattg aaaactttaa
      241 aatctccaca caaaaaattt ctgacgtgcc gcagtttgtc cgcagtatgg tgatggtgaa
      301 aaaggcgacg gcacaggcaa acggtaagtt gggcgcggtc aagcccgaaa tcgccgccgc
      361 cattgaaaag gcttgcgacg aagttctgct gaacaaccgc tgcctcgacc aattcccctc
      421 cgacgtgtat cagggcggtg cgggaacttc ggtcaatatg aacaccaacg aagtcatcgc
      481 caacctcgca ttggaagcct tgggctatga gaaaggccgc tacgatatcg tcaatccgat
      541 ggatcatgtc aacgccagcc aatccaccaa cgatgcctac cctaccggct tccgccttgc
      601 cgtgtattac agcatcggcg aattgctcga caaactggcc gtcctgaaaa acgcctttgc
      661 cgccaaagcc gaaaagttta aagacgtttt gaaaatgggc cgcacccagc ttcaagatgc
      721 cgtgccaatg actacaggac aagaattcca atctttccaa gtgttgttgg aagaagaaat
      781 cctcaacctc gaccgcacgc gccaactgct gttggaagtc aatttgggcg caacggcaat
      841 cggcacgggc gtgaacacgc ccaaaggcta cgccgaactg gtggtcaaaa aactctccga
      901 agtcagcggc ttgccttgca aactgaccga aaacctgatt gaggcgacct ccgactgcgg
      961 cgcatatgtg atggtacacg gcgcattgaa gcgcacggcg gtcaaactct ccaaaatctg
     1021 caacgacttg cgcctgctct cttccggccc gcgtgccggc ttgaaagaaa tcaacctgcc
     1081 cgaattacag gccggttctt ccattatgcc tgccaaagtc aatcccgtga ttcccgaagt
     1141 ggtcaaccaa gtctgcttca aagtcatcgg caacgacacg acgattacct tcgccgccga
     1201 agccgggcaa ctgcaattaa acgtgatgga gccggtcatc gcccaatgta tgtttgaaac
     1261 catttccctc ttgggcaatg ccgcagtcaa cctgtccgac aaatgcgtca aaggcattac
     1321 ggtcaaccgc gaaatctgcg aacgttacgt tttcaactcc atcggtttgg tgacttatct
     1381 gaatccgtat atcggacacc acaacggcga tttggtcggc aaaatctgcg cccaaaccgg
     1441 caaaggcgtg cgcgaggttg tcttggaacg cgggctgttg agcgaagaag aactcaaccg
     1501 catcctctct cccgagaacc tgatgaaccc ccatctgtaa cgttgcagac aaatgccgtc
     1561 tgaagcaggg atactgcttc agacggcatt ttgttatatc aatcagccgg attatatgcc
     1621 gaacagtgcc ttgacctccg cccaataaaa caggcaggcg atggaacaca gtgccaacac
     1681 gattcccgcc gtattgattg tgttgatttt ttccttaaaa aacagtgcgc ccgaaagcgt
     1741 ccccaaaaca atcacgccga tattcatacc ggcaaaaacc agcgtgggat tgtccttcat
     1801 catttggtgc gcggtgatgt aggttacgat gttcatgaag ttcaaaccgc ccaagagtat
     1861 gccgccgacc acactctcaa cgcgccatct gaccgatttg gcaaacaggc aggcaaacat
     1921 cagcacacct gccagcacaa atgcaaccag caggttgcct gaaaatgccg tgccgctttt
     1981 ggcaagctgt ttgaacagga tgtcgataat gccgtaaccc gcccacacgc ccagcagcaa
     2041 tgccgcctgc cgccacgcgc tgcctgattt tttgccgccg ccgtgtttcc aaagcaggca
     2101 gaacagcgcg gcaaatgcga ggcacagccc gattagtctg ccttcgctga gtttttcgcc
     2161 aaacagcgtc aaggcggcaa caatcggcaa aaacagcgac aaacgctgcg ccgcgtcgga
     2221 tttgacgata ccggcggatt ctacggattt gcccataatg acaaatacgg acggcagcag
     2281 cacgcccaat gcggcaaaca gccaccatgt cggcaaaaat gcgccgatat tgccgacatc
     2341 cggcttcaat accagcaggg tcagtatgac cgcgaccaca taattgacgg cgaccgcctg
     2401 cgcgatgtcg atttttttct tccttgccat tttcagcaaa atggaaaccg acacgctgaa
     2461 cacgatgctg attaacaggt aagccatact atatccattt ctcgggtgca accgccctgc
     2521 cgccgttgaa gcggataaag ccggcgatga tgcggtagaa ataccagacg ttcgttgcaa
     2581 taataatcag cacgccgata cccaaaaggg cagtcagcgc acccaaaata taaagccaaa
     2641 atgtgcgcca aaaggtttta atcaggtatt cggtatgtgc ggcatagaca atgctgtcca
     2701 aactgttccg tttcatatag gcgagaatca cgccgactat gggcatatag gcgaaaatca
     2761 cgccgactat gggcatgaac agcatgattg cacccaaacc gaacaggatg taggcggcga
     2821 ggacatagtt gcgcccgctt ccgccgccgg acggttcgta agcatggcgc gaacgtgagg
     2881 cctgccgttc aaacgcctgt ctgaacgcct gttcgcggcg catctgttcg cggtaaaact
     2941 gctccctgcg cgcctgttcg cggcggaatg cctcttcctg ccgcccgcgt tcctcattgc
     3001 ggtaggcgcg tttgatttcg tccgctcccg cctgcggcga cacgcccaat acggcataca
     3061 aatctttgtc catattgttc ctttatactt tcccgtcaaa ccgccgcctg tattgttttc
     3121 gggttcaaaa tatttaaatc tccgggacgg atgccgataa tgaacccgcg ttttccgccg
     3181 ttgatataga tggtttccaa atccatcacc gactgttcga cgtaaatatc caactttgtc
     3241 cggatgccga acggcgttgt gccgccgacc agatagcccg tccacttgtt tgcctgtacg
     3301 ggcgtggcgg attcgatgtg tttcgcaccc aaatgccgcg ctagattgcg ggttgaaatc
     3361 tgcttgtcgc cgtgcatcaa gacaatcagc cccttaccgt tttcatcttg caaaacaatg
     3421 gttttaatga ccaagtgttc gtctttgccg aatagtcggg caaactgcgc cgtgccgccg
     3481 tgttcctcat aggcataggt aaaaggttca aattcgatgc cgttttcacg caaaacgcgc
     3541 acggcaggtg ttatcggata agtgtgttta ctcatttttc gtcatgcaaa acatggaaat
     3601 cggtcaagcg caatgttacc atatatcccc tttatcgaag gaaaccgata tggatatccg
     3661 atatttcggc acaacgcccc gctattccga agccgtcggc gcaaacggct tgattttcct
     3721 ctccggcatg gttcccgaaa acggtgaaac ggctgccgaa cagactgccg acgtgcttgc
     3781 ccaaatcgac cgctggctgg cggaatgcgg ctcggacaaa gcgcacgttt tggatgcggt
     3841 catctatctg cgcgatatgg gcgactatgc ggaaatgaac ggcgtgtggg acgcttgggt
     3901 tgccgccggc agaacgcccg cccgcgcctg cgtggaagcg cggctcgccc gtccggagtg
     3961 gcgcgtcgaa atcaaaataa ccgccgtgaa gaaagatgcg gcaaccgcat aaacagattt
     4021 cctttatatt tcagagtacg caaatgatga aaaccttact gccctttttc ggcgtggtac
     4081 gcgtcagcgg cgaagaccgc caaaccttcc tgcacggaca gctttccaac gacatcaata
     4141 atcttcagac cgggcaggcg tgttatgcga cttacaacac gcccaaaggc cgtgtcatcg
     4201 ccaatatgat tgtcgtcaac cgaggcggcg atttgctgct gattatggcg caagacttgc
     4261 ttgaagcaac ggtcaaacgt ttgcggatgt ttgtgttgcg tgccaaagcc gtttttgaaa
     4321 tccttgaaga ttacgccgtc gacgcggaat tggaagcatc cgccgaaccg cttgccgcgc
     4381 aagaacccag tctcgtcttt accgccgaat gcgtttcaga cggcatctgc accgtcgtcc
     4441 tgccccatcg gggcattttg cacatcgccc ccaaaaacgc cttgccgcct tatgatgccg
     4501 ccgccgaaaa cgcgtggcgg ctgcacgaaa tccgcagcgg ttacccgtgg atatgcgcgg
     4561 ctaccaaaga aaccgccgtc gcccaaatgc tcaaccagca catcatcggc ggcgtacact
     4621 tcaaaaaagg ctgctatccg ggacaggaaa tcattgcccg tgcccagtat cgggggcagg
     4681 tcaaacgcgg cttggcggta ttgtcgggaa attcggcagc cgaagcaggc atcctgctgg
     4741 cggcagacgg cgaagaggcc ggcatcgtac tcgacagcgt tcaagattcg gaaaacttta
     4801 ccgcccttgc cgtgattaaa ttctccgccg cacaaaaaac actgtccgcc ccaaacggcg
     4861 gcagcttcaa agcggttcat ctcttcttta aaaccgaaaa tgcagaataa gccgccgatg
     4921 ccgtctgaag cccttcagac ggcattgccg ccccgtcaat cggttacaat gccgcctttc
     4981 taattttcaa acccaaaccg ccatgtccaa aaaaaccaaa caagaactcg aaaacaacaa
     5041 actcagcaaa cgcctgcgcc acgccgtcgg cgacgcgatt aacgatttca atatgatcga
     5101 gccggacgac aaaatcatgg tctgcctctc cggcggcaaa gacagctatg ccctgttaga
     5161 catcctgcgc cagctccaag ccagcgcgcc gattgatttt gaactggtcg ccgtcaatct
     5221 cgaccaaaag cagccgggtt tccccgaaga agtattgccg acctatctcg aaagcatagg
     5281 cgtgccgtac aaaatcgtcg aagaagacac ctactccacc gtcaaacgcg tattagacga
     5341 aggcaaaacg acttgttcgc tgtgcagccg cctgcgccgc ggcatcctct accgcaccgc
     5401 aaaagaattg ggctgcacca aaatcgcctt aggacaccac cgtgacgaca tccttgccac
     5461 catgttttta aatatgtttt acggtggaaa actcaaagcc atgccgccca agctggtgag
     5521 cgacaacggc gaacacatcg tcatccgtcc gctggcgtat gtaaaagaaa aagacttaat
     5581 caaatacgcc gaattaaaac aattccccat catcccgtgc aacctctgcg gctcgcaacc
     5641 caacctgcaa cgccaagtca tcggcgatat gctgcgcgat tgggacaaac gctttcccgg
     5701 ccgcatcgaa tcgatgttct ccgccctgca aaacgtcgtc ccttcccacc ttgccgatac
     5761 cgaactcttc gactttgccg gattggaacg cggtcaaacc ctgaaacacg gcggcgattt
     5821 ggcgtttgac agcgaaaaaa tgcccgaacg tttttcagac ggcagcgaag aagacgaaag
     5881 cgaaatcaaa atcgcgccgc aaaaagccga acgcaaagtc atcaacattc tggcgaacaa
     5941 gccgaaaacc tgcggcgcgt aaccccccgc cgtcaaaaat gccgtctgaa gcccgtttgg
     6001 cgtttcagac ggcattttaa aatcccgaag cagacggctt ccaaacattt gtttctcagt
     6061 gtccgttttc agaatgcccg cctgcgggca tattttgccc gatcagttcg gctatcctct
     6121 cgccgtcaaa ctgcgttttg aacaccacct tgatttcttt ggaaatctcg gcaaacagct
     6181 tttcggcatg tttgattttc cgctcttcgc tttttcgcaa atcttccgcc ccgtcagtcc
     6241 ctttggcttc aatcacaaag ttcagaatct cgccgctttt ggttttcacg atataggcaa
     6301 aatcgggcga atacgtgccg ccgccggcaa cggggatttt gatggagttt ctcggtattt
     6361 tggtgaatac ggttacgcct tcaatttggt tgttggcgac attttcatgt tctatatccg
     6421 aatcgtagaa aatttcgtcg aagagatagc cggcggcagg ccggtgctcc gtatcttcaa
     6481 atctgcccaa atctgctttt ttcaccgcgc gcggtttgcc gtctttatcg gtaaatttgg
     6541 tcggatggat tttgctgccg acaagccggt aatccagttc aaatttatgg aaggaatgat
     6601 gaagcaaaaa ccggttgaag ccgtttttga tttgggcgat ggtctgcata ttcaaaaaat
     6661 cgccaatgtt cagttcgtcg cggacgcgat aaaacgcctg atgcaaagtc tgcatgcgga
     6721 tttttgccgt ttgtgccagt ttttccagaa gctcacggta agtcattgtg ttaaaatgga
     6781 taaaatcttc atcttcaaaa ctgtctatgc ggcgggaaag cataagcccg ttgttgatat
     6841 aagcttcgtt taccgccgtg cgtatgcctg cctgcgggaa tttggcggcg ttttcacgca
     6901 aataggcggt aaataaatcg acaaattcgg cttcatcctt gattttgtac tgcaaaacgg
     6961 ctttatggtg aatcagctcc cacaaggctt tgagttcttc atatttgcct tcgcgcatga
     7021 tgatggtgtc tttgccctcg tctttggcgt tgctgacttt gcctttgtcc aaacctttgg
     7081 ggaaggcttc gggataggcg gcttttaatt tgtcatagcc gtcttcggca aagttttcat
     7141 tgtcgtcaat gatgccgtct gaaaacagtt ggtttaccaa taccagcggt ttgatatcgg
     7201 ggtatttttg caatattttt tgtttcagct cttcggtaaa ctttttggag atttcttcct
     7261 gaaaagaatt gtcgttgatt tcgccgacaa gctgcttcac aaagtctttt tcgctgctat
     7321 cgacaaaata attcagtttg tacggtacat cgcgcacccg cgccatcagc tcgtttaccg
     7381 gcaggcgcag gccgcgtccg acttcttgca gcttggaagt cgtgctgccg ctggaacgca
     7441 gtttgcaaat ctggaaaacg ttgggattgt cccagccttc gcgcagcgtc catttggaaa
     7501 aaataaagcg gcgcgggttg tccaaagaca gcagtttttc cttatcgtgc aggatttcat
     7561 tgatttcctg ctcgattcta tcgtcgctgt ctgtattgtc tttggaaaaa tagccgccgt
     7621 ggcaggcgga tacatcgtcc aacgtctttt gcaggtaatc gcggtaaaac gggtcgcttt
     7681 ccgttttcag acggcgtgcc gcttccgcgc gaatccagct ttcaaattta tctttcaggc
     7741 tgcctgaaag ctcgttgccg ctgcggtagc ccgcgatatc gtcaataaaa aacagcgtca
     7801 gcggcttgat tttgggctgt ggcgcgcgtt ctgccaaaag cgcgcgttcc agcttgaaat
     7861 gttcggcaac cgcccgctgc atcatcgcat cctgcaccgt ttgcgaatag gaataagggt
     7921 tgatgacggc acccgttttc aactccaagc cgttgcttaa caccaccacg gttttattca
     7981 ttttgtcgat tttcaaatcc gaaatagccg gatggatttg cgccaaatct tcgccttttg
     8041 ccagtttgaa cgtctgcttt ttgtcctttt cgtttaattc aaatttcgct tctttgccgc
     8101 ccgacgacac cagttttacc gccgcatcca tgccgccctg catttcttcc tgaaacacgc
     8161 gcacgccttt gaccagcccg tcgttaaacg cgtctactgc cgtcaaacgg taaagcaagt
     8221 tgtaatattc atcgttaaat gtcgcaccgt agcgcaaaat atattgcggt tttaagcgtt
     8281 tgatattgcc ccacgttttc gcgctctccc gggtcgggaa tttatgcggt tcgtccacaa
     8341 tcacaaacgg gcgcacggcg gccaatgcat caacgggatt gtcaaacaaa tctttcaatg
     8401 ccttgtcgcc cgtatcgttc atggacgacg aattaaccat gcccgcgtta atcagcagca
     8461 catggatttc ctttttgttt tccgctttga caaattgctc aatcgttatg ggcgcattgg
     8521 actttttgcc cttattcttt ttcgcgcttt ccaccacata ggttttcagg cgcacgcctt
     8581 cataatcgcc gccgaaatcc tgttcaaaat gctctgccaa agccttgctt tgcaaaaact
     8641 gctgtgttcc cgccttaatg gacaaagtcg gcacgaccac gataaatttg aacacgccca
     8701 gccaacggtg cagctcgaac atggtttgtg tgtaggtata ggttttgccc gtgcccgttt
     8761 ccatggaaat atcaaggata ttttggtcgt ccgaacggtc ggggaatcgg ccgtctatac
     8821 cgttttggct ttgcactttc aggatattgt ttgcgtattg ttttgcagca aacaaaagtt
     8881 cgggattttc gtctgccgtc cgatatttgg gcgttgcccc gtcaaacacg cccaaaaccg
     8941 ccgaaaccgc ccgcatttgg tgcggctggt ttttctcgta attaaaaccg ctcatgaatt
     9001 gcctccgtca aaccctgata accacattca actcaatctc ttttttattg gcataaccgc
     9061 gaaccgcctg gtcaagttcg tgctgcatgg cgcttgccat attgctgccg aatacaatca
     9121 cgcggttggg attgaaagcc gcatcgtcgt ccagcttgcg gataaacgcc aacaaatcgg
     9181 cagaagtaaa accggcattc atcagataca gccgtttttc gcacagatac gccgtgtaag
     9241 cccctaaccg cacaggctca accggcgtgg tcagtgccgc cccgtcatac agcgtccagg
     9301 tggtcagaag cgtttgcagc tgttcttcgc ttaattcatc gttaagcggc aaatccggtt
     9361 gttcgggcga aaaatccttg tccggatgct gcctgaaatt gtctgccgtt tgaaagattt
     9421 tgaagcccga atcgcccgtg taatcgggat gttcgacgcg gattttggcg gcggcttttt
     9481 ctatgcgggc tttggtgatg tcgaagatgg tcgggtagcc tgctttacgg gcttcggatt
     9541 tttcagcggt tttttcggga agctgtacac agatatagcg gcggttaccg ttttgtcctt
     9601 cggcgttaag ctgcatcacg gcgtgggcgg ttgtgccgct gcctgcgaag aagtctagga
     9661 ttaggtcagt ttgatttccc ttgaaagttg cagaaagaac taatcttttc aataaatcta
     9721 atggttttgg tgtttcaaaa ggagaataac cttttccagt ttcaaacaac tcttctaatt
     9781 cacgaatacc atctttagta agatttttac ttatatttcc aatatttaag atgctatgaa
     9841 atttggaaaa atcatcggat acttttctaa gttttccaat aattgttttc ccataagact
     9901 tcggaattaa gatatattca ttattttcaa tatctttatc aattttgttt ttaaactcaa
     9961 ttccttttat ttcaataata tcttcgtaat gcaagttccc atatttgcca aactgttcgc
    10021 gaacggcatc ttcctgtatc caatataatt catcattgat tacaattttt ttgccgcgta
    10081 tatcttttgg tctttttaga ttgtcaaaat gagtaatatc tttcgcataa actaaaacag
    10141 tttcatgccc atttacaaca tattttgcat taccaccacc ttctgatgta ttaacaatta
    10201 aatctttaat aaaattccct tccccaaaca cttcatcaca caacaatttc aactgcgccg
    10261 cttcgttatc gtcaatcgag ataaaaatca cgccgtcgtc ctttaacagt tcgcgggcga
    10321 catacaggcg cggatacata aaggtgagcc atgcgctgtg cgagtttgag cctttgtcgg
    10381 tgaaatctaa aatccgcgcg gcttcgtctt cgtcaatatt cgccaggcgg gcaagttcgg
    10441 ctggggtgaa tttgcggtcg tcctgataga caaagccgtc cgagccagtg ttgtagggcg
    10501 ggtcgatgta aatcatcttc acgctgtttg tataggcgtt ttttaagtgt ttcaacactt
    10561 ctagattgtc gccacgaatc agcaggtttt ggctgcctgc attttcgggc ttggcgttgt
    10621 gcgtcttatc ttcacttatc agggtttcgg gcggcaggtt gcgaagcagg cgggcatatg
    10681 atttgcccag ccagttcatt tcgtaaaatt cgcgtccgat gtcggtctgt ggcgcaattt
    10741 cggcttgtaa tctgtcgata aggaaatttc cgtctgcgtc aaaacaggcg ggaaacagtt
    10801 ttttgagctg ttcgagttgg gtagagttgg cggtaatgcc gtctgaagtg tagattgcct
    10861 cggtgttcgc cccggctgtg tcgcttagat cagggcttgg cttggcttgg cttggcttgg
    10921 cttggcttgg cttggcaaca ttttaaaatc ctcggtttga atttgtcaat atcaactgtc
    10981 tgttttaaaa tattttttta atttaaacgg cgttttttgg gaaacgggcg acgccgtctg
    11041 aacgtctgtc tgcgtgttac tgcccgacaa caacgcgacg gattttgacg ggctgtacgg
    11101 gtacgttttg ataaaagccg cgcgtggcgg ttttgacgcg ggcgattttg gaaacggcgt
    11161 ccatgccgct ttcgaccctg ccgaaaacgg tatagccgta ttgtccgttt ttgtagttga
    11221 gtgaatcatt gtccgccaga ttgataaaga attggctggt ggcggaatcc ggatcggccg
    11281 tccgcgccat ggcgatggtg ccgacagtgt ttttcaaacc gttgccggat tcgttggcaa
    11341 cggccttatc gcttgccttt tgtaccaagt cctcggtaaa tccgccgccc tggataacaa
    11401 aaccgtcgat aacgcggtga aaaatcgtat tgtcgtaaaa gccttttcgg gcatagcgca
    11461 cgaaattggc aacggttttg ggtgctttgg attcgtccaa aaccaaacgg atattgccca
    11521 tatcggtttc catcaaaaca tgggttgccg ccatagacgg cagggaaacc gccaaaagca
    11581 gcgcggttaa aacggttttg aatttgggtt tcatcccgtc ctcctcagac cttcagacgg
    11641 cattttcatt tcatatgccg tctgaaggcg cgtgaacgct attccaatgc gtctttgagc
    11701 ttttgttcga ttaaatccgc atcaaacgat ttggcaatca attcaaaacg cgaatcgcgc
    11761 cgccatgaga cttggttcgc gccccactgt ccgtctaccc agttgagcca cacccacgtt
    11821 cccaatactt gaaacacgcc tttggcgcgg acgagtcctt cggtcatttt gggcaggtcg
    11881 ttgaaaaagt tggtcagctt ttcgccgtcg aaatcgcgtc cggcggggaa tgtgaaacct
    11941 tgcgactgga agcccatggt attatccggc agggctttga ggcggtagcg tgatttttcg
    12001 atgacgggaa tatcgagcca ccggatgtcg agttgtgcgt tttggacttc aaccactttg
    12061 gctttgggcg ggaacagttt tgccgctttg tcgtgaaatt cggcaagctg ttcgggggtg
    12121 cataaatcgg ttttgctggc gaccaatacg tcgcagatgc cgatttggtc tttatacaac
    12181 gcctgttgcg cgtaatcggg gttgatgaac tggcgcggat tgacgacggt aaagacggcg
    12241 ccgatttcca aaaggctgtc cagcggtttg gctttgagtt cgtcgatgac gctggctgcg
    12301 tgagccagtc cgcttgcttc aatcatcagg cggtcgggct tggcgtcgcg cagcattttc
    12361 tgcacggtta cgcccatttg cgggccggag gtgcaacaca aacagccgcc ggcgatttct
    12421 gccacaggga taccgttgtc gctcaatacc gcgccgtcaa tgccgatttc gccgaactcg
    12481 ttgacgatga tgacccattt ttcgttcggg tctttttgcg ccatcaggct tttgagcgca
    12541 gtggttttgc ctgttcccag aaaacctgaa atcaggtgga ctttggtttt tttcatttct
    12601 gacatttttt acagattcca gttaaaacaa cgtgttcttc tttcagcgca aagccgcttt
    12661 cggcaacgcc tgcgcgcagt gccgcccact cgtggctgag ggtttgctcg tccgccgtgc
    12721 cgcattcggt gcagaccaaa ataaacgcgc tgtggtgcgc ttcggcttct tcgtggtcat
    12781 ggcaatggtc gtcgcactcg tgctgcgcgt ggctgcacaa aatatagccg ttgaccgccg
    12841 ccactttgtg caaaacgccc tgctccgccc aaaaatcaag ggcgcggtag gcggtaggcg
    12901 gtgcaagcac gccctcgctt tgctgctgca tctgcgacaa gacgttgtag gctttaatca
    12961 cgccgctttg ctgcaagaca atatctaata cctgctcgcg caaagcggtt acctgcaagc
    13021 cttcgctgcg tgcctgttcg ataattttct gtttgaaatt tgttttcata aattctctgt
    13081 ttatgccgtc tgaacaaccg atacggcagg aggcggtttt atatttgtat tcaattgctt
    13141 tatttggaaa tcttttccaa caatgcccga cagcccgcat ccgcaagcct gaaggtttct
    13201 tcaaaatcgc ccgtatacca cggatcgggg acatggtcgt aaccgctttc gggtatcagg
    13261 tcggtcagct tgaatatttt ttccggccgc ctgccgaagg ttctttccaa ttcggacaaa
    13321 ttcttgccgt ccatcgcgat gatgcagtca aacgccgccg catcgctttg gcggattttg
    13381 cggctggtaa agcctgaagc atcgataccg tgttttttca atatctttgc cgtctcgcgg
    13441 tgcatatctt cgccgtcgtg ccagcccgat gtccccgcgc tgtccgcttc aaggggaatg
    13501 cccgcttcgg cggcgcggcg gcgcaaaatg tattccgcca tcggcgaacg gcagatgttg
    13561 ccgaggcaga caaaaaggat tttcggtttt ttcatatccc ctccctgttc cggcgcgatg
    13621 ccgtctgaag cggaaaccct ttcagacgtc atcggtcggt taatcgtaac cgcataaggc
    13681 agaaccagcc aaaacatcca tctggaagaa atggtttggc taaatcttag gcatatttaa
    13741 taagtgtcca atattagaag ccgtatgctc caaatagaga ctggcatttt tcaaactatc
    13801 ttctaaaggt tcacttttct ccaaaataga aaaggcagct tggatatttt caaatggcag
    13861 ggaaggcaaa tcttcaacga gactgccaca aatagcgaca acaggaactc cgacaggggt
    13921 tctttttgct acaccaatag gcgctttccc tgctaaactt tgacgatcta gtcttccttc
    13981 accaacgata accaagtcaa catctgacac tttcttatca aagtcaatca agtccaggca
    14041 ggtgtcaatt ccagatacga tacttgcctg agcaaaggcg cacaaaccac cagcgatgcc
    14101 tccaccagct cctgctcctt taagttttaa tgttgcaggg gagacttttt cataaaaatc
    14161 ttgtattgcc tgatctacgg cctcaaacat agtagaatcc aacccttttt gcttgccaaa
    14221 cgtataggtc gcaccttggt gtccacataa gggactcaca acatctgcta aaatacgaat
    14281 gtgaacatct tcaggaattt tatagcgatt ttctgttgaa acagaagcta ggtttagtaa
    14341 ggattgaccg caagcgggta aggcatttcc atcctcatca taaaattgat aacctaaacc
    14401 agcagcaatc ccaatacctc catcattact ggccgtaccg ccaacgccaa tatagatttc
    14461 tttaatttct tgactaatga ggtggcgaat caattctcca ataccacgag tttggatttg
    14521 taatggattt cgtttctcta gcgggatttt tccaagacca accaaatcag caacttcgaa
    14581 tagggctagt tgttcttttt gaaaatagcg catgacttct ttttgtccaa aaggtcctgt
    14641 cacttggaga catttttctt ctaggtcaag agaatgtcgg atagcatcta cagtgccttc
    14701 tcccccatca ccgacaggac agaggagaca ttccacatct gctatcgatt gttggaagcc
    14761 tctttttatt gcttcagcta cctgttgagc tgtcaagctt tccttaaacg aatccggtgc
    14821 aattacaatc ttcatattta taattcatcc tttcgtttca ctcaaggcac aacacagaat
    14881 gaaaaagtgt tgtgctcttt attttgattt attatagaaa tgagaaagcc tatcactact
    14941 acaaatcact atgcgctgaa aaacggattg tgcccttccc gtttcaatgc ttccgcatag
    15001 ctcgggatgc tttcctgttc gcccaaggga ttgtgcaaca ggtaaacgtg ttctacacgg
    15061 cattctgcca ttaaatccaa aaaattttgc tgaataacgc caatattgtc ggtaaaggaa
    15121 agctgccacg aaaacacctg cggcacgact tcaaacgcgc cgtcggtcgc attaaacccg
    15181 aataaaatct gtccgcccgc ctttgccgct gcgaccacgc cgacgcgcgg actctgcata
    15241 cggacggcgc gtatcgcgtt tgccgcaaac acatccatcg ccatgcgctg gcgcgtgtgg
    15301 ctgccgtcgg aaagcgcgcg taccggagaa tccggggaaa gcggattggc aaacagcgta
    15361 acgccttccg atgcaagatt ttcctgccat acctgcatca agggcagacc tgcctgaaga
    15421 aggttctgac ccaatgccgt ctgaactttg ccgctgccga aacccagcgc gtaaacgacg
    15481 tggacggact gcccttcggg caaaagcaaa gggcgcgggg cgaaacggcg taagtgttgc
    15541 gccgccgctt cggtattttg tttggcacgc caccactcat ccggcgcgac cgcactcaaa
    15601 tcggaggaat gtacaagata aggcagccat tgcgtctctt gcgtcaacgc gcgcaggtgc
    15661 ggatagtttt gcaaacaggc aaacaatccc tccgtcggca gcttcatctc gattgcccgt
    15721 tctttcttgc agccagacac cagcacgacc ggcagggcga accactgcac ttcgccttct
    15781 ttctcgcaat cgagtaccgc gttcacactg gaaagcagcg cggcataagt tccggcatcg
    15841 ggcgacatcg tcagcgcgag ggaaaggttg atatagtggt tttgctcaag catccccctg
    15901 atttcggttt gaagttggcc ggacgagagt ttgcgcgaag cctgggaaga attatgcgcc
    15961 aactggtagg cattgagcag caggtggttt ttaatcggat tttggggata cgggcgcgta
    16021 tcgggcaagg taaatgtctg gttcatatat gtcgtgtacc gcttttcgca gtgtgtgttt
    16081 ttcctgtttg aaaatatatc ggacggattg ccgtcgggac ttgcccgtca atccgccgaa
    16141 acgagaaaat gcctgtctgc caagtctgcc aatatttctt ccacatacac ttcggcaggc
    16201 ggatggaatg tcaaaccgtc gggcgtggtg ctgacgatgt tcaagttttc ggcaaccgtt
    16261 tcaatcaatg cctgtttgct cgtcggccgc gacaattgcc gcataatgta caaatgcccg
    16321 tacccgaatg agggggtgct ttcgttgtaa cggttggcag gctggacaaa cgcttccgcg
    16381 ccgtgttcca caaaggcggc gacataatct gtaaaacgcg ccggcacata ggttttatgg
    16441 tcttcaaaaa attgtttgcc cgcattgtct gatgaaacag aaaaacgccc gagaatggtt
    16501 tgcaataaaa gctgggaatt gattttcata cggtcgaatg tcaaaccggg aaatcggcgg
    16561 gcgagatttt ccgcaacatc ttccgtttta aacgcctctc cggttttcat cacatcgcgt
    16621 atgcccgaaa gcagggtatc gttttcatca aagttgattg tttcccctct tgccgggcgg
    16681 aaattcaaac tttctatcac ttcgacggca accgactcgt cacgcctgac agtatccccg
    16741 acttcctcac ggcataaaag cgaacggcgg aattggcggt cggataaaat atcgctgtaa
    16801 aattctttgg caatataatc gccccctgcc aatgccagaa tccgctcccg cgtatgctcc
    16861 gccatccaag aaacaaaaga cgcgtgcaaa tcggtatccc cgatatacgc cagccggtgg
    16921 cggtttgccc attcgatgaa gccgttaacg taaatcgggt cgttgaatat ttccatatat
    16981 tcgtgtgcga cataataagt attgtgattc aatatttttt gaatcgccgg aagtttgccg
    17041 ccgccgccca agcctttgtc gttttccaaa atttccgcca gcgccttgag cgcgtccaag
    17101 cctttccgcg tccgcgcttc caagggttct tcaagcacat ccctgccggc aaagtacata
    17161 atttcgcgca actgctcctg ccgtttccag ccagggtaaa cattgtatga aatataggca
    17221 atgccgtgtt tggtcaggtt gttccggcaa atcgaaaaga ttttgtcttt aactgcgtca
    17281 ggcacccacg accaaatgcc gtggacgatg atatagtcga acttcccgaa tgactcatcg
    17341 acggtcaaaa tatctttttc ttccagacgc acatttttca agcccatttt ttcaatgatg
    17401 gcgttgccct gcgcaacctg cctgccggac aagtcgatac cgacaaattc cgcatccggg
    17461 taataaagag cctgcgtgat gatgttgccg cccatcgaac agcccagctc caagaccttg
    17521 gcattggcgg cgggcgcggg ctgcaaaccc atcatgcggg cgcgagcctc caaggcgttg
    17581 atggcggttt gggaaaatgc tttggattca tacatcaaat catcatatga atttttgatg
    17641 ttggacacgt ccggcacacc gttctccgtt gcagcacgcg caaaggcggc ttttttcttt
    17701 ttgttcggca tattttgttt gtctgaaggc actattgccc gcaagtttaa ccaattcatc
    17761 ctacccgttc aactaaatca aatgccatct gaaggcgcgg agcgtacttc agacggcatc
    17821 ggggaggcgc gaaggcttca gacggcattt ttgccgcttt atttcaacgc ggcatcatag
    17881 ccgccgtctt caacggcttc aatcaacgcc cctgcatcgg tttgcgcggg gtcgtatccg
    17941 acggtcgcac ttttgttttc aaggctgact tcgacgcttg ccacgccttt tacgccttcc
    18001 aatatccggg taacgctttt gacgcagccg ccgcagctca tgccgccgat gtcgaggata
    18061 agggtttcca tcattttcct ttcgttggaa ctgcaatctg acgggcgtta ttgtaagtcg
    18121 gggcgtgaac ttgggcaaac gcggaaacgg tacggcggtt tggaaaaaga cggacgcttg
    18181 cgcataatgg cggcaattcc catcaggacg acaacaatga acgcttcaca aaaaccctgg
    18241 ttgagcatca tcgccttggc aatcggcgca tttatagtgg attaacaaaa accagtacgt
    18301 cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca ccaagtgaat
    18361 cggttccgta ctgtttgtac tgtctgcggc ttcgttgcct tgtcctgatt tttgttaatc
    18421 cactatacaa cgtcggtatc ggcagcggcg cactgttggg acattgggta acgcaatact
    18481 cgggcatttc ctgcatcggc attgccggta tgctggtatc ggcggcaggt ttgtgggtct
    18541 gcctgaattt gaaccgccat attcgaacct gaccgcgcga tgccgtctga agcccctccc
    18601 gcccttcaga cggcattttg attgaagaaa tatccgcccc tgcttaaaat aacaggcttt
    18661 gccgtgtttt aacgcctatt ttttgttcca aggatggttt atgccgttgc tgtctgtcga
    18721 gttcgcactg ttctttctcg ctttcctgcc gatttactgg ggcttggcga aatacccgtc
    18781 cgcccaaaac ctgctgcttc tggctgccgg tatgggctgg ctctaccata tcggccccgt
    18841 atttgcggca atcatcgtcc tttattcctc ctgcgtgtac cttttgggcg aactgctccg
    18901 ttccgatcgc gaaaatacgc gccgcttttg gctggggtgc ggcattgccg cctcgctgac
    18961 cgtcttgggc tttttcaaat atttcgactt tttccggccg ctgattgccc aatatgccgg
    19021 aaaaggcggc gcaatcgaca tcctgatgcc gctggggctt tcgtattaca ccttccagtc
    19081 gctcgcctat cttgtttact gcttccgcgc cccgcacgcc gcgcgtttca gctggcacga
    19141 gctgctgctg cacctgagtt ttttccccac cgttacctcc ggcccgatta tccgcgccgc
    19201 cgcattcaaa agcgcagacg gcgagcaggc aggcgcattg gcgcaaatcc gtacccgcca
    19261 accgcgctca cccgtccgcc ccgcactcgc cgtttccctg attctgctgg gtattgccaa
    19321 aaaatggtgg ctggcgggga tgctcgcaga aaactgggtg tcgcccgtgt ttgaaaaccc
    19381 cgcccaattc aacggctggg gcgtattggg cagcgtgtac ggctatacct tccaactctt
    19441 cctggacttt tccggatatt ccgatttggt tatcggcatg gcaatgctgc tgggcttccg
    19501 actgcccaaa aacttctcag caccgcttcg tgctttaaac atccgcgcat tttgggacaa
    19561 atggcacatc agcctttcca cctggatacg cgactacatc tacatcccct tgggcggcag
    19621 caaaaaaggc tttttgcgga ctcagctcaa cctgatggcg gcaatggtgc tctcaggcat
    19681 ctggcacggc tacggctgga acttcctcat ttggggcacg ctgcacggca cggcactggt
    19741 gctgctcaac acgggcgacc gctatttcgg acgcgacgcg ctatgccgtc tgaaatacct
    19801 cgcgccgctc tcgtggctca tcaccttcca ttttgtctgc ctgacctttg tcgtcttcaa
    19861 taccgcaagc cccgacgatg caggcgcggt tttcactgcc ctctttgcca atgccaacgg
    19921 ctggaatgcg ccgcaacagg cagacatgct gctgcttacc tcgtttgcat ccgtcatgct
    19981 gctctaccct tacctgcaac gcgctttcga cggcgtggtc aaaggtttgg aaaaaatccc
    20041 gatgtggctg tggtttatcc ccgtttccgc cgtcctgctg ctgattatcg tattggcccc
    20101 ctcggggata cccggcttta tttatgccaa tttttaaggg tttggacatg aaaaactttc
    20161 tttccctttt cgcctccata ctgatgtctg ccctgattgc cgtgtggttc agccaaaacc
    20221 ccatcaacgc ctactggcag cagacctacc accgcaacag cccgctcgaa ccgctcgccg
    20281 cctacggatg gtggcggagc ggtgtggcgt tgcaagaaaa cgcctacgcc ctttcagacg
    20341 gcatcaaagc cttcctgtcc ggcgaaacgc cgccgacggc tcaagacggc ggttcggcag
    20401 atatgccgtc tgaagccgcc gcatccgaag ccgtccctca aaccggtgaa atccaagaat
    20461 ggaaacagaa caccgacacc gccgccgtcc gcagcggcga caaagtcttt ttcgccggcg
    20521 actcgctgat gcagggcgtt gcccccttcg tgcaaaaaag cctgaaacag caatacggca
    20581 tcgaatccgt caacctcagc aaacaaagca cggggctgtc ctacccctca ttcttcgact
    20641 ggccgaaaac gattgaagaa accctgaaaa aacatcccga aatcagcgtg ctcgccgtct
    20701 tcctcggtcc gaacgacccg tgggatttcc ccgttggcaa acgctacctc aaattcgctt
    20761 ccgacgaatg ggcgcaagaa tacctgaaac gcgtcgaccg catccttgaa gccgcacaca
    20821 cgcactacgt ccaagtcgtc tggctcggca tcccctacat gaaaaaagcc aagctcgacg
    20881 gacagatgcg ctacctagac aaactgcttt cggaatattt gaaaggcaaa atcatcctga
    20941 ttcccaccgc gcacaccctg agcggcggga aagaccgcta caccgactcc gtcaacgtca
    21001 acggcaaacc cgtccgctac cgcagcaagg acggcataca ctttaccgcc gaaggacaaa
    21061 aactgctggc ggcaaaaata atggaaaaaa tcgtttttga accaagtacg caaccatcaa
    21121 gtacacagcc atgaacccca aacacctcat cgcattttcc gccctattcg ccgccacgca
    21181 ggcagaagcc ctacctgtcg cctccgtcag cctcgacacc gttaccgttt ccccgtccgc
    21241 cccctacacc gatacaaacg ggctgctgac cgactacggc aacgcctccg cctcgccttg
    21301 gatgaaaaaa ctccaatccg tcgcacaagg cagcggcgag accttccgta tcctgcaaat
    21361 cggcgactcg cataccgccg gcgacttctt taccgacagc ctgcgcaaac gcctgcaaaa
    21421 aacttggggc gacggcggca taggctgggt ttaccccgcc aacgtcaaag ggcagcgcat
    21481 ggcggcagtc cggcacaacg gcaactggca aagcctcacc agcaggaaca atagcggaga
    21541 cttcccgctc ggcggcatcc tcgcccacac cggcagcggc ggcagcatga ccctgaccgc
    21601 atcggacggc atagcaagca agcagcgcgt ttccctgttt gccaaacccc tgcttgccga
    21661 acaaaccctg accgtcaacg gcaacaccgt ctccgccaac ggcggcggct ggcaggtact
    21721 ggatacgggc gcggcactgc ccctgaccat acacaccgaa atgccgtggg acatcggctt
    21781 catcaacatc gaaaatcccg ccggcggcat taccgtttcc gcgatgggca tcaacggcgc
    21841 acaattaacc cagtggtcga aatggcgtgc cgaccgtatg aacgaccttg cccaaaccgg
    21901 cgccgatcta gtcatccttg cctacggtac caacgaagcc ttcggcgaca acatcgacat
    21961 tgccgatacc gaacagaaat ggctggatac cgtccgccaa atccgcgaca gcctacctgc
    22021 cgccggcatc ctcatcatcg gcgcgcccga atccctgaaa aacacgctcg gcgtatgcgg
    22081 cacacgcccc gtccgcctga ccgaagtcca acagatgcag cggcgcatcg cccgtcaggg
    22141 gcagacgatg ttctggtctt ggcaaaacgc gatgggcggc gtttgcagca tgaaaaactg
    22201 gctcaaccac ggatgggccg ccaaagacgg cgtacacttt tccgccaaag gctaccaacg
    22261 gtcggcggaa atgctcgccg acagcctcga agaactcgtc cgcgccgctg caatcaggca
    22321 ataatcggac aggaggcgga cggtatttcc gcaacagggg gatgccgtct gaaacgcata
    22381 ccttcatatt gcttcagacg gcatagccac cccgcgcacg gtttgccgga cgcaaccggc
    22441 attcgcctca agcatcggaa ggacgggggc gaacctccgg catacggcgc aaaggcggcg
    22501 tttgatatgc cgtctgaagg caaagatgat aaactgccgc cttccgtttt cagacg
//
LOCUS       DAXHTY010000025        22304 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXHTY010000025 DAXHTY010000000
VERSION     DAXHTY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22304
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.025"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            1..99
                     /locus_tag="ACFNMK_000799"
                     /pseudo
     CDS             1..99
                     /locus_tag="ACFNMK_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(120..1034)
                     /locus_tag="ACFNMK_000800"
     CDS             complement(120..1034)
                     /locus_tag="ACFNMK_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6364106.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEYFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="ACFNMK_000801"
     CDS             1290..2759
                     /locus_tag="ACFNMK_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HFC6364107.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="ACFNMK_000802"
     CDS             3011..3382
                     /locus_tag="ACFNMK_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364108.1"
                     /translation="MNKTLSILPAVILLGGCAVGGGNTFGSLDGGTGMGGSIVKMAVE
                     SQCRAELDRRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLDPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /gene="thrC"
                     /locus_tag="ACFNMK_000803"
     CDS             3455..4864
                     /gene="thrC"
                     /locus_tag="ACFNMK_000803"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC6364109.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPA
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDTTIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="ACFNMK_000804"
     CDS             4910..5704
                     /locus_tag="ACFNMK_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364110.1"
                     /translation="MTLPMQETCFSILLDELAAKQETAIASHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLAGIPLDAQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDE
                     ENISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSEQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="ACFNMK_000805"
     CDS             5892..6668
                     /locus_tag="ACFNMK_000805"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6364111.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7335..9806)
                     /locus_tag="ACFNMK_000806"
     CDS             complement(7335..9806)
                     /locus_tag="ACFNMK_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6364112.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGASTVDESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGITHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9803..10981)
                     /gene="hflX"
                     /locus_tag="ACFNMK_000807"
     CDS             complement(9803..10981)
                     /gene="hflX"
                     /locus_tag="ACFNMK_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC6364113.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGNPVIVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFISDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(10986..12266)
                     /locus_tag="ACFNMK_000808"
     CDS             complement(10986..12266)
                     /locus_tag="ACFNMK_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC6364114.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            12268..12396
                     /locus_tag="ACFNMK_000809"
     CDS             12268..12396
                     /locus_tag="ACFNMK_000809"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364115.1"
                     /translation="MPSSRRGNGVFYQNGKLANAVSDCRLPNRQTFPRADAEPDAV"
     gene            complement(12419..13144)
                     /locus_tag="ACFNMK_000810"
     CDS             complement(12419..13144)
                     /locus_tag="ACFNMK_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC6364116.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMNQINDCGRRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13217..13894)
                     /gene="radC"
                     /locus_tag="ACFNMK_000811"
     CDS             complement(13217..13894)
                     /gene="radC"
                     /locus_tag="ACFNMK_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC6364117.1"
                     /translation="MSIKQWPEGERPKEKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGTGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVKELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            13921..14043
                     /locus_tag="ACFNMK_000812"
     CDS             13921..14043
                     /locus_tag="ACFNMK_000812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364118.1"
                     /translation="MPSETDVPTRQISEMPSEPDFRFRRHLYFKTVLRRFFQLD"
     gene            complement(14017..15366)
                     /gene="gshA"
                     /locus_tag="ACFNMK_000813"
     CDS             complement(14017..15366)
                     /gene="gshA"
                     /locus_tag="ACFNMK_000813"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC6364119.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15626..17035
                     /gene="leuC"
                     /locus_tag="ACFNMK_000814"
     CDS             15626..17035
                     /gene="leuC"
                     /locus_tag="ACFNMK_000814"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC6364120.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRGLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17132..17386
                     /locus_tag="ACFNMK_000815"
     CDS             17132..17386
                     /locus_tag="ACFNMK_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364121.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17448..18089
                     /gene="leuD"
                     /locus_tag="ACFNMK_000816"
     CDS             17448..18089
                     /gene="leuD"
                     /locus_tag="ACFNMK_000816"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC6364122.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLI
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18263..19084
                     /gene="dcm"
                     /locus_tag="ACFNMK_000817"
                     /pseudo
     CDS             18263..19084
                     /gene="dcm"
                     /locus_tag="ACFNMK_000817"
                     /inference="COORDINATES: protein motif:HMM:TIGR00675.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            19090..19365
                     /locus_tag="ACFNMK_000818"
     CDS             19090..19365
                     /locus_tag="ACFNMK_000818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364123.1"
                     /translation="MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGG
                     QRIKDESLPKGYNIVSGKKSFEINKILDPNDVAANLGCNGYGAPFRR"
     gene            19343..19534
                     /locus_tag="ACFNMK_000819"
     CDS             19343..19534
                     /locus_tag="ACFNMK_000819"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6364124.1"
                     /translation="MEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSFDIPKKDRCDLLG
                     NTVVVPVIKAVSERLLHTL"
     gene            19550..20281
                     /locus_tag="ACFNMK_000820"
                     /pseudo
     CDS             19550..20281
                     /locus_tag="ACFNMK_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
     gene            20315..21385
                     /gene="leuB"
                     /locus_tag="ACFNMK_000821"
     CDS             20315..21385
                     /gene="leuB"
                     /locus_tag="ACFNMK_000821"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC6364125.1"
                     /translation="MTKHIAILQGDGIGPEIVAETVRVLDKLIAQGLDASYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLNILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21548..22111
                     /locus_tag="ACFNMK_000822"
     CDS             21548..22111
                     /locus_tag="ACFNMK_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC6364126.1"
                     /translation="MKKIIFAALAAAAISTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPIANLQSGSQHFTDHLKSADIFDAAQYLDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMEKTEVCGGDFSTTIDRT
                     KWGMDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5373 a         5882 c         5797 g         5252 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagc
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatactc caacttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggcg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 aattttccgg atgatggtta ttaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggcg gtaatcttac tcggcggctg
     3061 cgccgtcggc ggcggtaaca cattcggcag cttggacggc ggcacgggta tgggcggcag
     3121 tatcgtcaaa atggcggtcg agagccaatg ccgcgcggaa ttggacaggc gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cccccgaacg gatgaccggc aacgatgtga tgcagatgct
     3301 ggatccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaatc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tatatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcccccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 agcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg caatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcgaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaagggcgt gaacgtattt atgttgtcgc ccgacggcaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggatattgtg aaggcagtgc aaaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac agtcaactcg attaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcgggcta tttcaaggcg acgcaaagca atgacgagca
     4201 ggtcagcttc tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac acatcgccaa
     4261 acagatgggt ttgcctatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cgtaccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccgtcg atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctgcggaaa tcaatacgct gtgggcggaa gtggcagcgg gaaaaggttt
     4501 tgacctgcgg tttgctttgg aaaaagtcgg cggcaaatac ggctttactt ccggcaaatc
     4561 cacccacgcc gaccgcctcg ccaccatcaa acaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgtgaag aaggggaaac
     4681 ggtggtttgt ttggaaaccg cattggcggc gaaattcgat acgaccatac gcgaagccgt
     4741 cggcgatgtc gccattccgc gccccgccgc actggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatattcc gtgcagaagc attccgcccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acctgttttt ccatactgtt ggacgaattg gcggcaaaac aggagaccgc
     4981 catcgcctcc cacctgcttg ccgacgggag caaggtatgg atacgcaaag caggcaggca
     5041 caatgcacga tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gtccccagcc tcggcggcga gcctgccatt gcaaccgaat caaaacgcct
     5161 gtacgaattg cgcgcggcgg ggatagccgt tcccgaattg ctcgccgtca ggaaaaatgc
     5221 ccttatgttc ggcaatttgg caggcatccc gctcgatgcg caaatccgcc aagaagccga
     5281 agccggaaag gcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acagcagttc ctcagtcagg cgtttgcgcg gaatatgatg tcggacgagg aaaacatcag
     5401 ctttttggat tttgaagacg acccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacatcc attcgaccgc gctgatattg aaaaacggcg gactgctgga
     5521 agcggctgcg gaaaaatggg gcggcgtact gtctgagcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cccgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 gtaagccgcc gcctgccccg cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttggcttta tcccgaatcg
     5821 ggcagacatt tccactctgc cgcacgtccc gaacaagcac tatccatttc ttttactgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggacagt
     6001 tcgttatggt cggattgatg gtggacggca agccgctgat gcgtgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaaccctggt tgccggcgac ctgaatccgg gcaaacacct ttacttgttg agcaccggta
     6241 ccggcatcgc tcctttcttg agcatcacca aagaccccga aatttacgag caatttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gcgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcccgagaa gaattcgaac accacggccg cctaaccgac ctgatggtaa
     6481 gcggcaagct gtttgaagac atcggcctgc ccaaaatcaa cccacaagac gaccgcgcca
     6541 tgctgtgcgg cagccccgcg atgctgaaag atacctgcaa agttttggac gatttcggac
     6601 tgaccgtctc tccgaaaaca ggcgtgcgcg gcgattacct gattgagcgc gcatttgtgg
     6661 atcaataagc tgcttgaaaa tgccgtctga acggcatcag cgataaaaaa acccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg ggtaaaaatt agaaatgcac atttccgtca
     6781 ttccaacaca ggtgggaatc cggacttacc tgtttcatta tcgtttgaaa ataaaagaga
     6841 aaccaaacat atagattccc gcttgcgcgg gaatgacgac ggtgttttgc cgtagccaaa
     6901 gcagaaaaac atccctattg tcatttcatc atgatatgac aataggatgt taaaaattaa
     6961 ttatcattat atgattgata tataagaaaa actgtcccaa taagaaatgt tatgcagaaa
     7021 ataaaatgaa aaggatggtt ttcattgaaa atggttgttc tgccaccgtc atacacagag
     7081 ccagtatttg gaaaaaaatg atccatattg acatcattat tccatttgtc gcataaatcc
     7141 actttttaat tttttccaca ttaatataat ctcttattta ataaaatcaa aataaaaatc
     7201 aaatgcctaa caattcgatc tcaaattttg aacgccaacg tgtttgagcg gtaacaatac
     7261 atactttaag tttgcaatcc ttcatcgtct tatttttgtt ttcatcaact atacgtctaa
     7321 agcaacgccg tccgtcattg ttcggacggc attttttcag actgcatttt cccccgtttg
     7381 tgaaggcgca gggcgttgcc caaaaccgcc agcgaactga agctcatacc cagtgcggct
     7441 atccacggtt ggacatagcc caaaacggca agcggtacgg caatgatatt gtacgcgccc
     7501 gcccatatca ggttttgccg gataatatgg cgggtgcgcc gcgcctgatc gagcaggtgg
     7561 gcgacggtac gcaaatcttc gttcaataac acaatgtccg cgccgtccct cgcaatatcc
     7621 gtcccgcccg ctgcggcggc ggatacgtct gcctgcgcca aaacgggcgc gtcgttgatg
     7681 ccgtcgccta tcatcagcac ttttttccct tctttttgca aggctttgac gtattccagc
     7741 ttgtcctcgg gcatggcttg ggcgcggtag tgcgtgatac ccagggcgcg ggcggtttcg
     7801 gcaacggcgg tttcgcggtc gccgctgagg atgtgcaggg tcaggttttt gcctgccaac
     7861 tgccgcaccg cctccgccgc gctgtctttc aaggggtcgg tcaggtagaa cacggcttgg
     7921 aaaccgcttt gactgccgag gtaaaccgcg ctgccgccgc cttctgtttg cggttcttta
     7981 cctgaaattt cggcgacata ggatgccctg cccaatgccc aaacctgtgt ttcgccgttg
     8041 acggtcagtt gcgcgcccac gccttcgccg atgcggttga ggcgttgttt aatagcaatg
     8101 tcggggacgc tgccgcctga aatgcggcag ttgaggatgg cgcgcgcgag cgggtgttcg
     8161 gactgctgtt ccaaactttg cgccactgcc agcacttggg cttcggtcat gctgccgagc
     8221 agttcaatcc ggcggacggc gggattgcct tgggtcagcg tgcctgtttt gtcgaaaatg
     8281 atgtcggtgg tttgggcgag ggtttcgatt gcctgctttc cgccgattaa aataccttcg
     8341 cgcgccagcg taccggtaga agctgccagc gcggtcggcg tggcaagcga taaggcgcag
     8401 gggcaggtaa tgaccagcag ggcgacggta atccacaatg cggtgtgcgc gtcggcgtac
     8461 agcgtccagc cgatgaagac tgggacggca agcaggagtt cgccgaatat gaaagacgag
     8521 gcatattgtt ccgccaactc ggcggtgcgc ggtttttgcg ccaaggcgcg gtcgagcagg
     8581 cggacgatgt gcgacagtcg cgtgccgccg ccggtgcggt cggtgcgtat aatcaggggg
     8641 ctttgcgtgt tgagtgtacc tgcggttact ttttcagacg gcattttggc gacgggcagg
     8701 ctctcgccgg tcagcataga ttcgtcaacc gtgcttgcgc cttccagcac cgtgccgtca
     8761 acggggatgg tttcgcccgg tttgaccagc acgatatcgc ccgccttcaa tttgacgaca
     8821 gctgcctcgc aggtttcctg cgtatcgggg taatcgggca tatgatggca aaacgcagga
     8881 atcagcttca ccagcctttc ggcggcatcg cctgccttgc gtcgggcaat gtgttccata
     8941 aagcgtccgc ccagcaggaa aaacagcagc atggctatgg attcgaaata catcccctgc
     9001 cccgcattgg tcgcaaggct gtaaacgccg gcgataaagg tcatgatgat ggcgacggta
     9061 atcggcgtat ccatgccgac gcggcggttt ttcaagtcgc gcagcgcgcc ttgataaaac
     9121 gggacggcgc aatagaatac gacgggcagc accattaaaa agccgcccca atggaggatt
     9181 tgcaggaaat cgggttcgat gtcgccgccg taaaggtagg tcggcagcgc gaacatcatc
     9241 gtctgcatca tccccagccc ggcaacggcg aggcggacga tgtattgttt gcgttctttt
     9301 tggttggcgg cttcgatttt ttgcgcgtca tagggtgcgg cggtgtagcc tatctgcctg
     9361 attttcaaca gaatgtcgga aaggcggatt ttgccgtcgt cccagacgac gcggcagcgg
     9421 tgcgtgctgt aattgaggtc gatgcggacg atgccgtctg tacgcaaaag ctgctgttcg
     9481 atcagccaga cgcaggcggc gcaggtgatg ccgccgagca ttaaaaccgc ctcgcgcgtg
     9541 ccgccgtggg tttccacaaa gtcggactgg acttcgggca ggtcgtacag gcggatttgg
     9601 tcgaggattt cttggggcgg cagctcggtt ttttgcgcgt cggcggtgcg ttgtttgtaa
     9661 taactgccca agcccgcgtc aataatgctt tgtgcgaccg cctgacagcc ggcgcagcag
     9721 gtttcgcggt cttcgttttc gtaacggaca gtcagatgca ggttttcggg aacatccaga
     9781 ccgcagtgga aacaggtttt tttcatggca tttcggtttc gtctgtgttt ggtgcggcgg
     9841 cacaagactc ggcaatggct tcgcgcagtg cgtctatacc ggtattttca gcaacggaaa
     9901 tgcggacggc ggcaattttt cccgcagcgt cgcgccatat gcccgtgttt tgttcttcag
     9961 acggcagcag gtcggttttg ttgtacacct tgatgcacgg aatatcgccg gcatggattt
    10021 cttgcagtac gttttccacg tcttcaatct gctgtccgct gttcggagcg gcggcatcga
    10081 cgacgtgcag cagcacatcg gcttgcgcgg tttcttccag cgtggcggaa aaggcggaaa
    10141 tcagtttgtg cggcagatcg ctgatgaatc cgacggtatc ggtcaggatg atgctgcatt
    10201 cgggactgat gtacagccgc cgcgccgtgg tgtcgagtgt ggcgaaaagc tggtctttcg
    10261 catatatgcc cgacttggtc agccggttga acaggctgga tttgccgaca ttggtatagc
    10321 cgaccagcgc aaacgttttg attgtgcccg attcgcggga cttgcggcgc agggcgcgct
    10381 gttttttgag gttggcaagc tgttttttca aggcgttgat tcgatgggcg atcaatcggc
    10441 ggtcggtttc cagtttggtt tcgcccggcc ctttcatgcc gataccgccg cgctggctct
    10501 gcagatggcc gtaaccgcgt atcaagcgtc ccgccaaatg gctcaattgc gccaactcga
    10561 cttgcagcct gccttcctgc gtgcgggcgc ggcgggcgaa aatcgccaaa atcaacccta
    10621 ccctgtccaa tacgcggcat tggaggattt tttccaaatt gcgttcctgc gtgggcgtaa
    10681 gttcgtggtt gaatacgacc aaatcgatgc cgtctgcggc aactgcttcc gacagctccg
    10741 ccgccttgcc cgtgccgaca aacagcgcgg tgtgcggacg gtcgcgtttg gcggtctcca
    10801 cgatgacggg attgccgccc gccgccttga ccagctcgac ggcttccgcc aatgccgtct
    10861 gaaagccgtt cagacgggcg gcgctgctgc ccgtaccgtc tttgtccaac attacgccca
    10921 ccagcatgac gcgttcgggt tgcgcttggg tactgccgtt gccgcctgtt ctgcctgtca
    10981 tgtattcaat cctgtaaata aagggttacg cctcgttccg tttctccttc cggtgcaagc
    11041 gtgatgcggc tgcccgccat tccgtcaacg ctgaagacga tgccgcccgc ctcgtgcctt
    11101 cctatggaca ggcggctgcc gggtcgctgc cggtaggtat aacgggtaca cgcttccaaa
    11161 caccagcgtc cgcgccagtg gatgaaatat ttttcgcctt ttctaaccgc aagtgtctcg
    11221 ccgccgttta cctcaaaata agggtaatcg ggttttcggg ttctaaactg ccatttcgcc
    11281 tgcgcgcgcc gtccgttgcg gacatagtcg aataccgccg tataaagcgt accgtattcc
    11341 aaaggcttga gcgggaaaag cgcgaattgg tacgcggtca gcctgccgtt ggggtcgttg
    11401 ccggcggtta aaaccctgac ggggcggatt tcgtttttac cctgatacag cttgaaactt
    11461 ttcatcgtaa ttttgcctgc cgcctcggaa aaatcaatgc tggcaggatt gcccgtgatt
    11521 tcatattccg gcacggggtc tggacgctcg ccgtggaaat aaggcagtgc gccgttgccg
    11581 acgggatagg ctgtatagag cagctcctgt gcgggcatgg cttcgtcggt gtacacgacc
    11641 gcaccgttat ggcaggcgtt gcggtaatat ttccgtcctg cttccggctg atttctgcct
    11701 tgggcgcaat gccgctcaaa cctgccgttg ccctgattga atacgagaac ggttttaccg
    11761 ttttcgcgca caaatgccgc tcctgcctca tccgtatggc ggtcaagtag ggaaaggcgg
    11821 tggtagattg cgctcattaa tccgtccact tggcgttgct gcgtgcggat gtcgctgtcg
    11881 gacgattcgg cggcttcctc ttccgtgctg atgttttcat gcacgccgtt gtagagatac
    11941 ccggcaaggc gtgtgcgttc ggtcagcttt tgtgcggtgt agtgcggatt gtcgggatgg
    12001 tgttcgccgt gtccgtcttc gggattgagc gtgagatagc gtgcgtgcct gcgggcggaa
    12061 ttttccaaaa ccggcgcgtg tgccagcttg tgcaaaccga tttgggcgcg gatgcggttg
    12121 aggtagtgca gcgcatcgcc gccgtccctg atttgcggtg cggacggata gacaagttcg
    12181 cccgcgggca gggattggtt ttgggtatga tagaaaacgc ctgccgccga acccaacagg
    12241 agcagccgaa taaaaaggga tttcataatg ccgtcctccc gacgcggcaa cggggtgttt
    12301 tatcaaaacg gcaaacttgc caatgcggtt tccgattgca gattgccaaa ccgacaaacc
    12361 tttccccgtg ccgatgccga acccgatgcc gtctgaacct tcagacggca tcgggtgttt
    12421 atttgtccac tcggatgggt gcaggttcgt attgtgtcga ttcgtccccg taatacagca
    12481 cgccgagttt gatggggatg cgtccctgcg atttgcggtg ggcgttggaa tcacgcaagg
    12541 aataggcgca gccgcaatat tcctgctggt agaagttttc gcgtttgctg atttcaatca
    12601 tgcgcgcgct gccgccgcct ttgcgccagt tgaaatccca atacaccaca tcatcgtaag
    12661 gcgcggcggc gcggcggcca cagtcgttaa tctggttcat gtttttccaa cgggaaatgc
    12721 ccagcgagct ggtgaagacg gggaacccat gttcatgcgc gtattgcgcg gctttttcaa
    12781 aacgcatatc gaaacacatc gtacagcgga tgccgcgttc cggctcaaac tccatacctt
    12841 tggctttggc aaaccattct ttgcggtcgt tttcgtaatc gtcgtctttg tcgatgaagg
    12901 ggatgccgaa cttttccgca aagcgcacgt tttcctcttt tctgagcata tactctttgt
    12961 gcggatggat attgggattg taaaaataaa tggtgtagtc gatgccggag gcaagcatcg
    13021 cctccatcac ttcgccgctg cacggggcgc agcaggaatg cagcaagact tttttatgtc
    13081 cgccgggcgg gacgagtatg gggcggtcaa tgtcggtaac ggtaggtttg ttttgtgttt
    13141 ccattaactt aacaatatct gtcgaatacg gttgaagcgg ctattttatc agagccgccg
    13201 catgtaaaac agggtgtcag ggcatcagcc cgagctgcct gaacgaacgg acggtttgcg
    13261 ccgttacgat aaaatggtcg agcagcgaca catcgaccag cgacattgcc tgtgccagcc
    13321 gccttgtgaa catgatgtct tcctgcgaag gttcgggcga gccgcccgga tggttgtgcg
    13381 cgataatcag gctgtcggca tactcgtcca aagccagttt gacgatttcg cggatgtaaa
    13441 tcgtgttttc cgcaaccgta ccgcgcgaca gctctttgac cgcaatcagt tggttttggc
    13501 ggttcagcag cagcgcgacg ctgacttcga ctttttcctg ccccaaatga aagcgtaaat
    13561 aatcggccac cgtatccgga tcggacagga cgatgctctc ctgcaattct tccgccaata
    13621 tccgccgccc gatttccctg accacggcaa actgtgtgaa acttgccgtc cccgtccctt
    13681 tgtatgccga cagtttgccg acctccgcgc tcatcagcct ccccaaactg ccgaactcct
    13741 gcagcaaata acgcgccaaa tcgaccgcac tcattccgcg cgtgccgacg cgcagcagga
    13801 ttgccaaaag ttcggcatca ctcaacgccg ccgccccgcg ttccaacagc ttttccttgg
    13861 gtctttcgcc ttccggccat tgcttgatgc tcatgttttc ccttttttaa atagtctgag
    13921 atgccgtctg aaacagatgt tcccacacgc caaatatccg aaatgccgtc tgaaccggat
    13981 ttccgcttca gacggcatct gtatttcaaa acagtcttac gccgtttctt ccaactcgat
    14041 tgaagccgcc agaagcgaca ggcgtgccat tacgccgtaa acgtagaggc ggttgtgttc
    14101 gttgtccacg tcgcagtctt tttcagagcg cggcataccc agcgagtccc attgttcgaa
    14161 tacgcgcttg caggcttcgg gcgcttcttg ggaattatcg ccgttaccgg taggaatgct
    14221 gttagacagc ggaacaaaca ccataccgcc ggcgttgagg ttttcgtccg caccgcgccc
    14281 ttcgtgtacg cggaaaaagc cgccgatgac gaagcggtcc atcatataca cgacgggttc
    14341 gcacaccgcg ccgtttaagg tttcataagt ataaatccct tcttggacaa tcacttcgct
    14401 gacttccaaa ccttctttga cttttgccat tttgttgcgg tttttacggt tcaagccgcg
    14461 cacttcatcc gcagatttga cgctcatcac gcccatgccg taagtgccgg catcggcttt
    14521 gacgatgacg aaaggtttgt cggtaatgcc cgattcgtcg tatttggctt gaattttcgc
    14581 cagcacacgt tctaccgctt ccgccaacgc gtcttcgcct tcacgccctt ggaagtccaa
    14641 accgccgatt ttttcaaaat aagggttaat ttgccattcg tcgatgccga ttaacttggc
    14701 aaattcggcg gtaacttggt tgtacgcgcc gaaatgattt gttttgcggc gcgtcgtcca
    14761 accgccgtgc aacggcggca aaacggtttg gctgatgcct ttgaggatgt cgggaatgcc
    14821 ggcagacaaa tcgttgttca acaaaaccac gcaaggcgaa aagccgtctg caagatggac
    14881 gcggtcgcgg gtacgcaata aaggttccaa caggattttg tcgcccaatg cagtttcaaa
    14941 ttcggtcggt tcggttactt ccggattcag gctgcccaag cgcacttcat accctgccga
    15001 acgcaaaatc tcgccgaggg cgtaaatgtt ttgcaggtaa aacgtattgc gcgtgtggtt
    15061 ttcgggaata atcaatacgg atttcgccgt ttcgcaggcg cgttgcaccg catcttgcgc
    15121 ggcaaccgcc gccagcggga taaagttggg attcagatta ttgaagccgc cggggaacaa
    15181 attcatatcg atagacgaaa ttttgtaacc ggcattgcgt atatcgaccg aaccgtaaaa
    15241 cggcgggcgg tgcgcgttcc actgcgtgcg gaaccacgcc tcgattttgg cgtgattgga
    15301 caggattttt gcctcaaacg cctgaagttg cgccgaatgt tcgggcgaca tcaccggtaa
    15361 tttcatcttt cgtctgcctc tgtgtgtttt ttgacaagat gcagatgata atgcctttga
    15421 aagaatgacg acaagtattc tttcgtatcg aatcatgctt tagatataac tgtttactga
    15481 aatggacaat atgtctaatt ttttcgttaa aattttttgc aaaataacac cggctcaaaa
    15541 attagacaaa atttgttgcg cggtataaag aatacgtcta aaatccgccc aatccgcatt
    15601 accatttatc caaaagaaca acatcatgac agcacaaacc ctctacgaca aactttggaa
    15661 cagccacgtc gtccgcgaag aagaagacgg caccgtcctg ctctacatcg accgccattt
    15721 ggtgcacgaa gttaccagcc ctcaggcatt tgaaggcttg aaaatggcgg ggcgcaagct
    15781 gtggcgcatc gacagcgtcg tctccaccgc cgaccacaac accccgaccg gcgattggga
    15841 caaaggcatc caagacccga tttccaagct gcaagtcgat actttggaca aaaacattaa
    15901 agagtttggc gcactcgcct attttccgtt tatggacaaa ggtcagggca tcgtacacgt
    15961 tatgggcccc gaacaaggcg cgaccctgcc cggtatgacc gtcgtctgcg gcgactcgca
    16021 cacttccacc cacggcgcat tcggcgcact ggcgcacggc atcggcactt ccgaagtcga
    16081 gcacaccatg gcgacccaat gtattaccgc gaaaaaatcc aaatccatgc tgattgccgt
    16141 tgacggcaaa ttaaaagcgg gcgttaccgc caaagacgtg gcactttaca tcatcgggca
    16201 aatcggcacg gcgggcggca cgggttacgc cgtcgagttc ggcggcgaag ccattcgcgg
    16261 cctttcgatg gaaggcagga tgaccctgtg caatatggcg atcgaagccg gtgcgcgctc
    16321 aggcatggtt gccgtcgacc aaaccaccat cgactacgta aaagataaac ccttcgcacc
    16381 cgaaggcgaa gcgtgggaca aagccgtcga gtactggcgt acgctggtgt ctgacgaagg
    16441 tgcggtattc gacaaagaat accgtttcaa cgccgaagac atcgaaccgc aagtcacttg
    16501 gggtacctcg cctgaaatgg ttttagacat cagcagcaaa gtgccgaatc ctgccgaaga
    16561 aaccgatccg gtcaaacgca gcggtatgga acgcgccctt gaatacatgg gcttggaagc
    16621 cggtacgcca ttaaacgaaa tccccgtcga catcgtattc atcggctctt gcaccaacag
    16681 ccgcatcgaa gacttgcgcg aagccgccgc catcgccaaa gaccgcaaaa aagccgccaa
    16741 cgtacagcgc gtgttgatcg tccccggctc cggtttggtt aaagaacaag ccgaaaaaga
    16801 aggcttggac aaaattttca tcgaagccgg ttttgaatgg cgcgaaccgg gctgctctat
    16861 gtgcctcgcc atgaatgccg accgcctgac cccgggtcaa cgttgtgcct ctacttccaa
    16921 ccgtaacttt gaaggccgtc aaggcaacgg cggacgtacc cacctcgtca gccctgccat
    16981 ggcagccgca gccgcagtga ccggtcgctt taccgacatc cgcatgatgg cgtaagtatt
    17041 ttcaggcggt ctttaagaat ttgacgcggc cgtctgaaat atgtcccgta aggcagaaac
    17101 acctttaata ttcaaaccaa ggagtttaac catgaacaaa cttttcatta ccgccctgtc
    17161 cgccctcgcc ttgtccgcct gcgccggcac ttgggagggc gcgaaacaag acaccgcccg
    17221 caaccttgac aaaacacagg ccgccgccga acgcgccgcc gaacaaacag gcaacgccgt
    17281 cgaaaaaggc tgggacaaaa ccaaagaagc cgtcaaaaaa ggcggcaatg ccgtcggacg
    17341 cggcatttcc catctcggcg gaaaaatcga aaacgccacc gaataaccga ttcgcgatgc
    17401 cgtctgaaac cgcttcagac ggcatcgtca gcacaagacg caaacccatg aaagccttta
    17461 ccaaaattac cgccatcgtc gccccgctcg accgcagcaa cgtcgatacc gacgccatca
    17521 tccccaaaca atttttaaaa tccatcaaac gcagcggctt cggccccaat gcctttgacg
    17581 aatggcgtta cctcgaccac ggcgaaccag gcatggacaa cagcaaacgc ccgttgaacc
    17641 ctgatttctc gctgaaccag ccgcgttacc aaggcgcgca aatcctgttg acgcgtaaaa
    17701 acttcggttg cggctcttca cgcgaacacg ccccttgggc attggacgac tacggcttcc
    17761 gcgccgtcat cgcccccagc ttcgccgaca tcttctttaa caactgctac aaaaacggcc
    17821 ttttacctat cgtgttgacc gaagaacaag tcgatcggct tttcaaagaa gttgaagcca
    17881 acgaaggcta tcagctctcc atcgaccttg ccgaacaaac cctgatcaca ccaagcggcg
    17941 aaacattcac attcgacatt accgaacacc gcaaacactg cctcttaaac ggcttggatg
    18001 aaatcggtct gaccctgcaa cacgccgacg aaattcacgc cttcgaagaa aaacgccgcc
    18061 aaagccagcc ttggctgttt aacggttaag cctatgccgt ctgaaggccg ttcagacggc
    18121 attcctgata acatataatt gttgcggcta atgtaaacag aggggcgata aattgctttc
    18181 atcttttaag cgggcttagc gacactctaa attaatgcaa taatgcaact ctccatttca
    18241 aataaatttt tagattttta atatgcaaca aattaaattt attgacttat tttccgaaat
    18301 gagtggcatc aggaaaggat tcgaacaagc ctgtcggaaa cagtcggttg cttgcgaatg
    18361 tgtttttact tcagaaatca aaccggcagc tttggaagta ttgaagcaaa actaccctga
    18421 tgaagtgccg tatggagata taacgaaaat tgaaacaggg gatattcccg attttgacat
    18481 cctgttggca ggcttccctt gtcaggcttt ttctttcgcc tgaaaaagat taggctttga
    18541 agatacacgg ggaacgcttt tctttgatgt ggcaaggatt ttaaaggcaa aaaaaccaaa
    18601 aggttttatt ttggaaaatg tggaaggatt ggtgacacac gatagaaaag atccgacaca
    18661 aaaaataggg cgcaccctga ccgttatttt ggaaaccttg gaagcattgg gctactatgt
    18721 ttcttggaaa gttttaaatg caaaagattt cggcattccc caaaacagga agcgcattta
    18781 tctgacaggc agtttgaaat ccaaacccga cttatctttc gaaacaagcc caagtccgaa
    18841 attaaaaaat attttggaat cgggactgcc tactgaaagc agccctttca tcaagaaatt
    18901 gctaaaaaaa tttcccccgt ccgaactgta cggaaaatca gtaaaagaca aacggggagg
    18961 gaaaaacaat attcacagtt gggatattga attaaaaggc gcagtaaccg aagaagagaa
    19021 gcaattgtta aatatccttc taaaagaacg gaggaaaaaa aatgggcttc agaaatcggc
    19081 atagattgga tggatgggat gcctttgaca aaagcgcaaa tttcaacttt ctataaacac
    19141 cccaatctcc aaaatatttt ggacagcctg acagataaag gctatttggt tttagagcat
    19201 ccgaaacaaa aaattggcgg gcaaagaatc aaagatgaat ccctgcccaa aggctacaat
    19261 attgtttcag gcaaaaaatc ttttgaaatc aataaaatat tagatccaaa cgatgttgcg
    19321 gccaaccttg gttgcaatgg atatggagca ccttttcgtc gttgacaacg gcggtttgcg
    19381 tacacttacc ggaaaagaag ggttgcgctt attcggctat ccggacgatt attcgtttga
    19441 tattcccaaa aaagacagat gcgatttatt gggtaatacc gttgtcgtcc ctgtgattaa
    19501 ggcggtatct gaaagacttc tgcatacttt ataaaccgac agggataaaa tgataaaact
    19561 tactgcacaa caaatatttg ataagttgct ggatgaagaa aaaatcttat cggccaatgg
    19621 tcaaattaga tttttcttag gcgatgtgga tattatcgtc aaacaaaaag atgttgtcgg
    19681 caacatcatt caggaatggc tcggcggatg attgagaaaa agggaaattg aatttgatgt
    19741 ttcaaccaat acccaaatgc ctcccgattt ctttttaaat aaaaaagacc gcagcagaga
    19801 attgttggaa gtaaaggcgt tcaaccgaaa tgccggcccg ggttttgata ttgcagattt
    19861 taaaatgtat tctgatgaaa tcattcataa gccctatatg ctggatgtag actatttaat
    19921 attcggttac gatatggacg acaacggcaa tgtaaccatc aaggatttat ggcttaaaaa
    19981 agtatggcaa attacgagaa gcatggatgg gtgggcaatt aatcttcaag tcaaaaaagg
    20041 cgtggtgcat aaaatccgcc cgggtgtttg gtacagcata aataaaaaga atatgcctat
    20101 gtttgaatgc ttggaagatt ttgtttccgc aattgaagaa accgtttatc aaaacccggc
    20161 aacacggcat aacgcatcct tatggaaaaa gaaatttgag gaagcctata aaaaacacta
    20221 caaccgatcg atttccatac ctcgttggca cgaaattgca cacaaataca aaaagaaata
    20281 acccttcaac catcaaccca taaggaaact tgaaatgacc aaacatatcg ccatcctcca
    20341 aggtgacggc atcggcccag aaatcgtcgc cgaaaccgtc cgcgtactcg acaaactcat
    20401 cgcccaaggc ttggacgcca gctacgaata cgccccatta ggcggcgaag cctacgacga
    20461 atacggccat ccctatcccg aatttacgca aaacctctgc cgcaaagccg atgccgtcct
    20521 gcttggtgca gtcggttctc ctcaatacga caacctcgac cgtccgttgc gccctgagcg
    20581 cggcctgttg gcaatccgta aggatttgaa cctgtttgcc aatttgcgtc ctgctgtttt
    20641 atatcccgaa ttagccaacg cctccacgct gaagcctgaa atcgttgccg gtttgaatat
    20701 tttgattgtg cgcgaactca ccggcgacat ctatttcggc gaaccgcgcg gcatccgcgt
    20761 tctggaaaac ggcgaacgcg aaggctacaa caccatgaaa tacagcgaaa gcgaaatccg
    20821 ccgcatcgcc cacgtcgctt tccaatccgc ccaaaaacgc agcaaaaaag tctgctccgt
    20881 aggcaaagcc aacgttttgg aaaccaccga actgtggcgc gaaatctttg aagaaatcgg
    20941 caaacaatac cccgatgtcg aactctccca catgtatgta gacaacgccg ccatgcagct
    21001 cgtgcgtgcg cccaaacaat tcgacgtcat tgccaccggc aacatcttcg gcgacattct
    21061 ctccgacgaa gcctccatgc tgaccggctc catcggtatg ctgccttccg cttctttgga
    21121 cgaaaacggc aaaggcctgt acgaaccgtc ccacggctct gcccccgaca ttgccggaca
    21181 aaacaaagcc aacccgctgg ccaccatcct ctcgctcgca atgttgctgc gttacagcct
    21241 gaacgacgaa gcgcgcgcgc aacaagttga aaacgccgtc caaaaagtgc tgcaacaagg
    21301 cttgcgtacc agcgacattt acgaagaagg cacaaaactc gtttcctgct ccgaaatggg
    21361 cgatgcggta ctcgccgcct tgtaaaaaaa caaagcgcgg atcacaccgc gctttttcat
    21421 ccgccctttt ccgcccgccc cgttcgtcaa ccgcctgcaa acccaccgtt taccggcagg
    21481 aaatgccata aatccctgcc tatgttagga taaacgcctt taccggccca cacaaaggat
    21541 tatcatcatg aaaaaaatca tcttcgccgc actcgcagcc gccgccatca gtactgcctc
    21601 cgccgccacc tacaaagtgg acgaatatca cgccaacgcc cgtttcgcca tcgaccattt
    21661 caacaccagc accaacgtcg gcggttttta cggtctgacc ggttccgtcg agttcgacca
    21721 agcaaaacgc gacggtaaaa tcgacatcac catccccatt gccaacctgc aaagcggttc
    21781 gcaacacttt accgaccacc tgaaatcagc cgacatcttc gatgccgccc aatatctgga
    21841 catccgcttt gtttccacca aattcaactt caacggcaaa aaactggttt ccgttgacgg
    21901 caacctgacc atgcacggca aaaccgcccc cgtcaaactc aaagccgaaa aattcaactg
    21961 ctaccaaagc ccgatggaga aaaccgaagt ttgtggcggc gacttcagca ccaccatcga
    22021 ccgcaccaaa tggggcatgg actacctcgt taacgttggt atgaccaaaa gcgtccgcat
    22081 cgacatccaa atcgaggcag ccaaacaata aatcttgatg cgaatcagca caaatgccgt
    22141 ctgaacgtcc ttcaaacggc atttgtgccg tcttcatcaa accgccccgc caacggtaag
    22201 ccgatatggc ggagcacaat ccgccgaaag atatttcagc ggcaggcagg gcttgccgga
    22261 aagtccggaa agtatagtgg attaacaaaa accagtacgt cgtt
//
LOCUS       DAXHTY010000026        22019 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXHTY010000026 DAXHTY010000000
VERSION     DAXHTY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.026"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..981)
                     /gene="mafB"
                     /locus_tag="ACFNMK_000823"
     CDS             complement(<1..981)
                     /gene="mafB"
                     /locus_tag="ACFNMK_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6364127.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDNHDSRSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKSNIVPRAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGVGIGAITDSAVSPVTDTAAQQTLQGINHLGNLS
                     PEAQLAAATALQDSAFAVKDGINSARQWADAHPNITATAQTALAVAEAATT"
     gene            complement(1021..1983)
                     /gene="mafA"
                     /locus_tag="ACFNMK_000824"
     CDS             complement(1021..1983)
                     /gene="mafA"
                     /locus_tag="ACFNMK_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6364128.1"
                     /translation="MQTRLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAVYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVEADNSHEGYGYSDEAVRQHRQGQP"
     gene            complement(2167..2886)
                     /gene="pyrH"
                     /locus_tag="ACFNMK_000825"
     CDS             complement(2167..2886)
                     /gene="pyrH"
                     /locus_tag="ACFNMK_000825"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC6364129.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(3106..3960)
                     /gene="tsf"
                     /locus_tag="ACFNMK_000826"
     CDS             complement(3106..3960)
                     /gene="tsf"
                     /locus_tag="ACFNMK_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC6364130.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVISFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(4089..4817)
                     /gene="rpsB"
                     /locus_tag="ACFNMK_000827"
     CDS             complement(4089..4817)
                     /gene="rpsB"
                     /locus_tag="ACFNMK_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC6364131.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(4990..5223)
                     /locus_tag="ACFNMK_000828"
     CDS             complement(4990..5223)
                     /locus_tag="ACFNMK_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364132.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFT
                     ITVRRRNHLMRQQEQAESEQQRAQRQKDSGTKP"
     gene            5301..5882
                     /locus_tag="ACFNMK_000829"
     CDS             5301..5882
                     /locus_tag="ACFNMK_000829"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC6364133.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAELYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            5943..6533
                     /locus_tag="ACFNMK_000830"
     CDS             5943..6533
                     /locus_tag="ACFNMK_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC6364134.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            7070..8536
                     /locus_tag="ACFNMK_000831"
     CDS             7070..8536
                     /locus_tag="ACFNMK_000831"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC6364135.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(8724..9098)
                     /locus_tag="ACFNMK_000832"
     CDS             complement(8724..9098)
                     /locus_tag="ACFNMK_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6364136.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(9336..9635)
                     /locus_tag="ACFNMK_000833"
     CDS             complement(9336..9635)
                     /locus_tag="ACFNMK_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6364137.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            complement(9790..10569)
                     /gene="map"
                     /locus_tag="ACFNMK_000834"
     CDS             complement(9790..10569)
                     /gene="map"
                     /locus_tag="ACFNMK_000834"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC6364138.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            complement(10609..11259)
                     /locus_tag="ACFNMK_000835"
     CDS             complement(10609..11259)
                     /locus_tag="ACFNMK_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364139.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            complement(11267..11875)
                     /locus_tag="ACFNMK_000836"
     CDS             complement(11267..11875)
                     /locus_tag="ACFNMK_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC6364140.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLHQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(11939..12532)
                     /locus_tag="ACFNMK_000837"
     CDS             complement(11939..12532)
                     /locus_tag="ACFNMK_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC6364141.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFNHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAIKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPYPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            complement(12537..12884)
                     /locus_tag="ACFNMK_000838"
     CDS             complement(12537..12884)
                     /locus_tag="ACFNMK_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC6364142.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            12938..13813
                     /gene="rsmI"
                     /locus_tag="ACFNMK_000839"
     CDS             12938..13813
                     /gene="rsmI"
                     /locus_tag="ACFNMK_000839"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC6364143.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLAGRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMVELLPERRLMLAREITKTFETFLSGTVGEIQT
                     ALVADGNQSRGEMVLVLYPAQDEKHEGLSETAQNVMKILAAELPTKQAAELAAKITGE
                     GKKALYDLALFWKNK"
     gene            complement(13848..14155)
                     /locus_tag="ACFNMK_000840"
                     /pseudo
     CDS             complement(13848..14155)
                     /locus_tag="ACFNMK_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            14433..15587
                     /gene="obgE"
                     /locus_tag="ACFNMK_000841"
     CDS             14433..15587
                     /gene="obgE"
                     /locus_tag="ACFNMK_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC6364144.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPIGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYD
                     KPRWLVLNKLDMLDEEEAQARTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            15670..16719
                     /locus_tag="ACFNMK_000842"
     CDS             15670..16719
                     /locus_tag="ACFNMK_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6364145.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            17407..18828
                     /gene="cysS"
                     /locus_tag="ACFNMK_000843"
     CDS             17407..18828
                     /gene="cysS"
                     /locus_tag="ACFNMK_000843"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC6364146.1"
                     /translation="MTTIYNTLTRQKEPFSPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGKLTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGRLKALGGIIGLLQRDPTEFLQGSAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            18999..19778
                     /locus_tag="ACFNMK_000844"
     CDS             18999..19778
                     /locus_tag="ACFNMK_000844"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6364147.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKALSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            19765..20109
                     /locus_tag="ACFNMK_000845"
     CDS             19765..20109
                     /locus_tag="ACFNMK_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364148.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLISLPCLLIASKCLGWKGWRLLANVLACLAVCALLSVPAA
                     LLIAFTLQDLLK"
     gene            20119..20649
                     /locus_tag="ACFNMK_000846"
     CDS             20119..20649
                     /locus_tag="ACFNMK_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6364149.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(20878..21993)
                     /gene="asd"
                     /locus_tag="ACFNMK_000847"
     CDS             complement(20878..21993)
                     /gene="asd"
                     /locus_tag="ACFNMK_000847"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC6364150.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMQEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         5030 a         5312 c         6090 g         5587 t
ORIGIN      
        1 cgtagttgcg gcctctgcta cggcaagggc agtttgggct gttgcagtta tattcggatg
       61 ggcatcagcc cattgtctgg cggaattgat accgtctttt accgcaaaag cactgtcttg
      121 taatgcggtt gcagccgcaa gttgtgcttc gggacttaaa tttcctaaat gattaatacc
      181 ttgtagagtc tgctgcgcgg ctgtatctgt gaccgggctt actgcactgt ctgtaattgc
      241 cccaatccct actccttgaa aacccattaa aaaaggatta accgcacctt ggacgatgcc
      301 gcgaatatca tccatacggt tagcccacca atttttattg gggtcgcttt cccatatcag
      361 ttttcctgct tcatcagcac ggctgaaaaa accagaggcg gcaccggcat tttcttttag
      421 ccaacggtct gaaaatgggg ctcggggaac aatattactc tttgtttttg ttgaagttcc
      481 tttgacataa tagctgtata tatcccttgc tcctccgggg ggcggataat cgctgccttg
      541 cggtccgtca tatccatcct ccggatggat ttccgaccct gtccgatgaa gttggtaaac
      601 agtaaaacca ccgtctacac cgccgctgaa atcagaagtg cttcttgaat catgattatc
      661 gaacggactg tgcacttcat gtccatgtcc tgaaaaatgg gtttcatagc cgataacacc
      721 ttcaaatcct gtccgttcgt gtgtaatagg cagtatgggg ccgaccgcag ttgcatcaaa
      781 tgtttggacg gcgtaaaccc gatttttaac actgccgcga gcattaccaa acagatggta
      841 tttaccaccc ggttcgtagt gttttgcctg catatcatcg cgcaaacggg catccaaacc
      901 gttcgcatga ctaatgggga tttgcagcaa cgatattgct gctgcaaaca gcatcatgaa
      961 tttttgaata ggcaaattca ttttgttttc cttggtagca agcggttatg gtagtgtgaa
     1021 tcaaggttgc ccttgtctat gttgtcgcac tgcttcatcg ctgtatccat acccctcatg
     1081 actgttatca gcctctacgg atggggcgga gttacccgta tgattgccgt atggtcggat
     1141 atcggagaaa tcgaccatta atccttccgt cggtttaatt cctttgctta ctttatacgg
     1201 ccccatccac aatgcgtaat tttctttata gacagcttca aacgcattgg ttttgggttt
     1261 gatgagcaat tttttattgg ttctgtctac tgcgaaatat tccagttttg tttgggcttt
     1321 cagtgtttcg gcattgtata ggtgcatttc ggttctgttg cgtatcgttc cgaatacgtc
     1381 gatgttaata aacacatctg tatcggcatt ggcaggagaa acaacgtcta tgccgcgcag
     1441 gaaaaatacg gtctgtacca agtgggaaag aaaggcagtg tcgcgcgggt tagtcgtcaa
     1501 ggtttcattt cgataatccc ccatcccgcc aatatttaag cccagactgc ttttacttcc
     1561 gctaccgtct gattgggtgc gagagcgtgc aggggcatta agtgtagata aagaagtggt
     1621 taaacctgtc aaaccgcctg atgttgtttc agcggtggtt tcgtaacgtg gataggtgta
     1681 atcggtacgg acggcagggc tgtttatgta ttcgccacga atcagtgcat caatggagta
     1741 gcgaccccct gtcaaactgc ctgaaccttg gtcgcccata gttgcaatgt acaatgcaac
     1801 ttttcgtccg tgtaatgcct gtaaatccat gtctttaacg gcagctctgg cagaagcggc
     1861 cacaagttct tgttcgaccg caaagcgttt acctccgcca tgcgatggaa tacctgtcag
     1921 tgtcccgcag gcggataaaa taaaaactga aaaaagaata ggtatcagca gccgtgtttg
     1981 catagatttt ctcctttgat gaaaaacaaa ttgtatcaaa attgtaaata tagtggatta
     2041 aatttaaacc agtacggcaa ggcgaggcaa cgccgtactg gtttaaattt aatccactat
     2101 aatctccata taataaatgg ctgtctgaag cccatatgcg actatatctg ccgacactat
     2161 ggtcaatcag cagtgaacca gcgtaccttc gttttcaccg gttactacac gtttgagcga
     2221 gccttcttta gcaatgccga atactacaat attgagcttg cgttcgcggc agagggcgaa
     2281 agcggtggca tccatcactt tgaggttttt caacaaggct tcgtcaaaag taatggtttc
     2341 gtagcgtgtg gcggacgggt cttttttcgg gtctgcggtg tatacaccgt cgacgttggt
     2401 ggctttgagc atcacgtcgc agttcatttc cgcaccgcgc aatgcggcgg cagtgtcggt
     2461 cgtgaagaac gggttaccgg taccggcggc aaaaatcacg actttgcctt cttccaaata
     2521 ttggatggct ttaggacggg cgtaagtttc agcgatttgc tgcatagaca gtgcggattg
     2581 tacgcgcgct ttgatgccta aagtttcaaa tgcgtctttg agtgccaacg cgttcatcac
     2641 ggtcgccatc atacccatgt aatcggcagt ggcgcggtcc atgctgcctg cttgggcgga
     2701 tacgccccgg aaaatattgc cgccgcccac gacgatgccg acctgtacgc ccattttaac
     2761 gacttcggca atttcgccga cggtttggac gatggtgtcg tgattgatgc cgaacggatc
     2821 ggaacccatc agggattcgc cggagagttt cagtaatacg cgtttgtatt tgatttgctg
     2881 tgtcatggga taccttgctt tcttgaatgt cgtcggtcgg agtttgggcc gtctgaattt
     2941 tcaaacggtc ggaattacaa tgatatggtg tgtcgttcta ttattgtttt gttttcagac
     3001 ggtctgaata ttcggtcgtc ttaaaatagg taccgcaaac ggttttctca aaaaaaagca
     3061 ccctgattcg tttggaatcc aggtgctttc tttttcataa gtgccttaca ctttggcggc
     3121 tgcggctact tcggctgcgt agtcgacaac ggctttttcg atgccatcgc ctactttgta
     3181 gcgtacgaag ctgatcactt cagtgccgtt ttctttagcg aattgggcaa cagtttgatc
     3241 ggggttcatc acgaatgctt ggccgttcag agtgatttca gccaagaatt tacggatgcg
     3301 gccttcaacc attttagcgg cgatgtcggc aggtttgcca gaagcgatgg cttgttcggt
     3361 gtagatgtgg cgttcttttt caacggtttc ggcatctact tcggcttcgc ttacgcattg
     3421 tggtttagcg gcaacaatgt gcataccgat tttgcgtgct acgtcttcag agcctttgta
     3481 ctcaaccaat acgccttcgg tcgccaatgc gccgtggatg taggcaacca gttggttggc
     3541 agtgtcgatc acttggaagc gacggacaga catgttctcg cccaatttgg cgatgatggc
     3601 tttgcgttct gcttcaacca gttcgctcag ttcttcaaca gaagccggtt ttttctcggc
     3661 ggcggttttc gcaacgaagt tggcaaattc tacgaagccc gcgtctttag caacgaagtc
     3721 ggtttcgcag tttacttcaa ccaatgcgcc gacattgccg ttaatcgcgt aagccaatac
     3781 gccttcggca gcagtacggc cagccagttt accggctttc gcaccggatt taatacgcag
     3841 gatttcttcg gctttgtcga agttgccttc agcttcaacc aaggcttttt tgcattccat
     3901 catgcccagg ccggtagcgg cgcgcagatc ggcaaccatt tttgcagtaa tttctgccat
     3961 tttgaatctc ctagattttt gggaaatacg gaaaggattt tccgcattcg ggaatcgtgt
     4021 gccgcgaacg gacgttcaga cggcatattt gagaaaaggg gcataacgcc cctcttcggt
     4081 ttgccggatt actcggcagc ggcttcttgg gcagcggcta cggtttcttg cagcgcttgg
     4141 tttttgcctt ccaaaactgc gtcagcgatg ccgcggcagt acaggcggat ggctttggcg
     4201 gagtcgtcgt tgccggggat aacgtatttc acgccgtcgg ggctgttgtt ggtatcgact
     4261 acggcgataa cagggatgcc caatttttca gcttcaacca gagtaccttt ttggtagccg
     4321 gtatcgataa cgaaaatcgc gtcaggcagg cctttcatgt ttttgatacc gcccaaagaa
     4381 cgttccagtt tttcaacatc gcgttgcatt tccagaattt cttttttgct gaaaccgctt
     4441 tcggcagcat tttccaaggc tgcggttttt tcttccaggc gtttgatgga ttgcttaacg
     4501 gttttgtagt tggtcagcat accgcccaac cagcggtaat caacgaaagg cataccggca
     4561 cgggtcgctt cttcgcggat gatgtcgcgg gcttggcgtt tggtacctac gaacaatact
     4621 gtacctttgt tggcaaccaa acggcgtacg gcttcttgcg cgtcttggaa catcggcagg
     4681 gttttttcca agtttacgat atggattttg ttgcgcgcac cgaaaatgta ttgtgccatt
     4741 ttcgggttcc agaaacgggt ttggtggccg aagtgaacac cggcttcaat catctgacgc
     4801 atagtaattt gagacatttt atttccttga aagggttaag agcacacatt caatcgcata
     4861 gaacttgata ggtcacgcct attgtcaagc acccttcggc gaaagtgggc ataagtttta
     4921 aaaaatagtg tgtttccgaa atggaaaact gacggattat agcggataag atgccgtctg
     4981 aaaagggatt cagggttttg tgccgctgtc tttttgccgt tgtgcgcgct gctgttcgga
     5041 ttccgcctgt tcctgctgcc tcatcaggtg gttgcggcgg cggacggtaa tggtgaaaac
     5101 cgtgaacacg gtgggcagca ccgcccaaaa aaccaaataa atcaaggcgc gcgcaatact
     5161 cggctgcgcg gcggaataca taacggcaac aaaaagatag ccgatggcga cgatgtgcca
     5221 cattgtgtgc gtcctgtttg aatgttaaca aaatcgttat aatcgcaaca ttctaccgca
     5281 atcctgaaag gccgtctgaa atgaggaatg aggaaaaacg cgccctgcgc cgtgaattgc
     5341 gcgggcggcg ttcgcaaatg gggcgggacg tgcgggcggc ggcaacggta aaaatcaacc
     5401 acctgctcaa acgttatatt aaaaaagggc ggaaaatcgg cgtgtattgg ccgatgggca
     5461 aggaattgcg tttggacggc tttgtccgcg cggcgcaaaa acgcggtgcg gaactctacc
     5521 tgccttatat cgaaccgcgt tcgcggcgga tgtggtttac gccgtatcct gaaagcggaa
     5581 tggaacggga gcgcatacgg ggcagggcga ggttgaacgt gccgcagttt gcagggcgca
     5641 aaatccgcgt gcacggtttg tcggtattgc tcgttccgct tgtcggcata gaccgcgaag
     5701 gctaccgttt ggggcaggca ggcggctatt acgatgcgac gcttgcagcg atgaaatacc
     5761 gtttgcaggc aaaaaccgtg ggcgtgggct ttgcctgcca gttggtggac agactgtcgg
     5821 tcgaggcgca cgacctgccg ctggacggtt ttgtgtcgga ggcggggata ttgtgttttt
     5881 agccgttcag acggcatttt gaacggaaat gccgtctgaa ggtggaatat tgggagaaaa
     5941 ggatgtcgga aaagataata ctgccggtct tgtcgttggg tggcgtacgg ctcgaaccgt
     6001 tggatgtgca tcatgaaacg ggtttgcgcg aggcggtttg cgatggggaa gtttggaagc
     6061 tgggagtaac gtccgcgccc catccggata gggtggcaga ctatatcggg acagcattgg
     6121 caacgcgttt ggcgtttgcg gttgtcgatg aagaggcggg cagggtggtc gggacaacgg
     6181 cgtattatca ttttgaaccg cagattccgc gtttggatat tggattcaca tggtatgcgg
     6241 catcggcacg gcggacacgg ataaacacct gttgcaaaat catgctgttg gattacgcat
     6301 ttgatgtttt ggcttgccgt tgcgtgggat ggcgcacgga tattctgaac cttgcctcgc
     6361 aacgtgccat cgaacggctg ggtgcggaaa aagacggcgt gctgcgtatg catatgctcc
     6421 gtaaagacgg cagtgtgcgc gatacggtcg tgtacagtat gctgagggaa gattggtgca
     6481 aaaacaggga aatcctgacc ggcaggttgg cagggtatgg cgtgcaggtg tgaggcagta
     6541 ttttcaaatt tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttagctcaaa
     6601 gagaacgatt ctctaaggta ctgaagcacc aagtgaatcg gttctgtact atttgtactg
     6661 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctataaaaaa cttaacgcaa
     6721 tatgctgaca atcttttaaa aattaatata taaacaattt tgatttttta tctattaaaa
     6781 atttgatata tatagataaa taactatttt tatttttcat ttcgcagttt cccgacttgg
     6841 cgggcgacag aaaaactttg catttcccag taaaatccac tacattcacg ataacatatt
     6901 acaagcccct gaagcggtgt cggaagccat acttgcaacg cattccaacc ccgaaacagc
     6961 atactcggac acacaccatt gccggttttc cctgcttttg cgattcaggc gtttgtagta
     7021 cggcaaataa ccataagggc agaagatgcc tgttttttag gagtaaacca tggctgaagc
     7081 gacagacgtt gtcttggtgg gcggcggcat tatgagcgcg actttgggcg ttttgctcaa
     7141 agaactcgaa ccgtcttggg aaatcaccct gattgaacgc ttggaagatg tggcgttgga
     7201 atcgtcaaac gcgtggaaca acgccggcac ggggcattcc gcgctgtgcg aattgaacta
     7261 tgcgccgttg ggtgcaaatg ggattatcga tccggcgcgc gccctcaata ttgccgaaca
     7321 gtttcatgtc agccgccagt tttgggcgac gctggtcgcg gaaggcaagt tggaagacaa
     7381 ttccttcatc aatgccgtgc cgcatatgtc tttggtgatg aatgaagacc attgttctta
     7441 tcttcaaaaa cgttatgacg cgtttaaaac ccaaaaactt tttgaaaata tggaattttc
     7501 caccgatcgg aacaaaattt ccgattgggc tccgctgatg atgcgcggcc gggacgaaaa
     7561 ccaacccgtc gccgccaact actccgccga aggtacggat gtcgatttcg gacggctgac
     7621 gcgccaaatg gtgaaatatt tgcagggcaa gggcgtaaaa accgagttca accgccacgt
     7681 cgaagacatc aaacgcgaat ccgacggcgc gtgggtgctc aaaaccgccg atacccgcaa
     7741 ccccgacggg cagctcaccc tccgtacccg cttcctcttc ctcggcgcgg gcggcggcgc
     7801 gctgaccctg ctgcaaaaat ccggcatccc cgaaggcaaa ggctacggcg gcttccccgt
     7861 gtccggcctg ttcttccgca acagcaaccc cgaaaccgcc gaacaacaca acgccaaagt
     7921 gtacgggcag gcttccgtcg gcgcgccgcc gatgtccgtc ccgcacctcg acacacgcaa
     7981 cgtggacggc aaacgccacc ttatgttcgg cccttacgca ggcttccgtt ccaacttcct
     8041 caagcaaggc tcgcttatgg atttgccgct gtccatccat atggacaacc tctatcctat
     8101 gctgtgcgcc ggctgggcga atatgccgct gaccaaatac ctgctgggcg aattgcgtaa
     8161 aaccaaagaa gaacgcttcg cctccctgct ggaatactac cccgaggcaa accccgacga
     8221 ctgggaactc atcaccgcag ggcaacgcgt ccaaatcatt aaaaaagact ccgaaaaagg
     8281 cggcgtgctc cagtttggta cggagattgt cgcccacgcc gacggctcac tcgccgcatt
     8341 gctgggcgcg tcgccgggcg catcgaccgc tgtgccgctg atgatccggc tgatgcacca
     8401 atgcttcccc gagcgcgccc cgtcttggga agaccgtctg aaagagctgg taccgggtta
     8461 cggcatcaag ttgaacgaaa accctgaaag ggcggatgaa attatcgcct ataccgcgaa
     8521 agtattggat atttaagccg aatctttcaa gaaaagccgt acccccatat ggtgttgacg
     8581 ccgtccctgc cggttatgcc cttcatccgg cagggcaggt tttgatacac ggggcaggta
     8641 atcgtttttt cccttttgcg aataatggaa cggtcaaaaa aacagcgttt tcatttctga
     8701 aaacgctgtt ttttgttgcc tgattaacgt ggggaacagt ctttgaagac gatttggttg
     8761 tcaggtgcgg taatcataat gggctgtttg cggtaagatt tgccatccat cacgccggta
     8821 cccaaaacat aaccgccttc tttgccgtag aatgtttcca cattgtcgga tttatccaaa
     8881 ttgacaggca tttgcacacg tttgccgttg atgacggcgg aagcgtatgt ggtcagaccc
     8941 tgtttgttga agccgtaggt tactttgact tttttacctt gctggcagac gtagctgacg
     9001 gtttttttgg caacggtggg gttgttcgtg ccggcagcag cagccatact gctgagcgcg
     9061 attgcggaag acaggattgc ggtggtcaga agtttcattt ttattccttt ggatggatgt
     9121 ggagtttgcc cgtttcgagc tgtttgacat tctaatgtcg gcgtatcctg aaataaacag
     9181 gaaattggat aaagtttgta aaaaggcggg ttcgggcggt tcggcggttt gaggcagggg
     9241 cggcaggcgg gatacggcgg cctgtccggt gtttcagacg gcattgtttg cgtttttcgg
     9301 aaatgaaaaa ctgtcgggca tccggaggtt gcggtttaac tttgtttttt tctttcggac
     9361 ttggtctgtt tggacaattc ggcatacagg tcgggatggt gttttttcaa cagggcggcg
     9421 atttccatat cttttttgtt gactttcgcc aaatcgatgc attcgatatg gacgaggcgg
     9481 agcagttcgc gtacgggttt gggcattttg cggccggttt cgtagcgcga gcctccagac
     9541 tgggtaacgc cgaccttgcc ccagaagtct atctggttca gcccggtctt tttacggatg
     9601 gctttaacat cttctatatt ttcaaatatt ttcataaaac ttcctttaag tttgtctggt
     9661 tgcggggcaa attccgattt gctttgtttt cgtaatgtgc cgggtcaagc ctgtttttat
     9721 attatatatc atatctgccg tttctttcag acggcattgt ttgtttttat ggggcggata
     9781 cgtccggctt cagggtttgc cggaggcggg gctgacggtg aggatttcgt agccggtttc
     9841 ggtcaccaag acttcgtgtt cccattgggc ggagagggag cggtctttgg taaccaccgt
     9901 ccagccgtcg ttgaggatac gcaggtggcg tttgccttgg ttgatcatcg gttcgacggt
     9961 aaaaatcata cccggtttta gaacggggcc ctgtcctttt tttccgtagt gcaacacttg
    10021 cggggcttcg tggaaaccgc gcccgatgcc gtgtccgcag aattcctgta cgacggaata
    10081 gccggcgttt tcggcaacct gttggcaggc gtaacctacg tcgcccagtg tcgcgccggg
    10141 tttgacggct tctatgcccg ccatcatgga ggcgtgggtt acgtcgatca ggcgttgggc
    10201 gatgggggag actttgccga cggtaaacat acggctggag tcgccgtgga agccgtcttt
    10261 tttgatggtg aggtcgatgt tgataatgtc gccttctttg agcggcttgt cgtcgggaat
    10321 gccgtggcag atgacgtggt tgacggaggt gcagcaggat ttggggtagg gcgggttgcc
    10381 gtagtgcagg ggggcgggat agccgccttg gacgttgacg tggtagtcgt aaacgagttt
    10441 gtcgatttcg tcggtggtta cgccgggttt gacgaattgt ccgatgtagt cgagggcttc
    10501 ggcgacgagt ttgcccagct cgcgcatttt ttcgatttct tcgggggttt tgatgatgat
    10561 gccgttcata atgcttcctt ggattgcggg tgtgcctgcc gtgccgggtc agaagaggtt
    10621 gtcgagaatt tctttatggg gtttgggcgg cgtgtttttc ggggtgtccg gtttggtatg
    10681 gttttctttg ggcgtttctt tttctttggg tgtttctttg ggctgcgcct gtttttcggg
    10741 tgtttctttt cggggttcgg gacgggggac gtttgtgggc agtacggggg tttctttgag
    10801 tgtgttttca gcttgtgttc cggcgtcgtc cggctgcctg tcggtttgag ctgtgtcggc
    10861 aggttgcggt ttgacccggt ttttcttggg tgcggcaggg gacggcagtc cgctgccgct
    10921 ttcgtctgtg ccgtccggct gtgcgggttc ggatgaggcg gcgtggtggt gttcgggttg
    10981 ggcggcatct tgtccgacta cgccgtttgg cagccagaat tcggtttcgc gggggctgtc
    11041 ggtgtgttgc ggttcggttt ggagggtttt gccgccgttc gggttgaatg tgctgacgat
    11101 gcctattgcc aatgcgccga tggcggtgat acagatgagc aatacgacgc gtatcaggag
    11161 tttgggggtc agcctgaagg gtttgttcgg tttttttgcc attttgattg tgcttttggg
    11221 gtgtcgggca atgccgtctg aaggcggttc agacggcatt gccgagtcag cgttggacgt
    11281 agttttggta gagggtgatg actttttgta cgccgacggt ggtgctgact ttttgggtaa
    11341 tctgcgcctg ttcttcgggg gtgaggatgc ccataacgta ggttacgttg ccgtaggtaa
    11401 cgattttgac gcgcgcctgt gtggcggggc tgatgcccaa cagcgtggcg cggactttgg
    11461 atgtgttcca agtgtcgccg gcgatgtcgc cggcagtgcg cggcagggag gcgacggtaa
    11521 tgtagttgta cacgccttcg gcggcctgtt cggaacgtgc aatctgaccg acgaactgtt
    11581 tttcgccttc ggtggcgact tgtccgagca gcagcaggtg gcggttgtag ccgacgacgg
    11641 agatttgggg cgtgtagcct ttggtttggt tgttttggtg caggtaggaa cgggcggtgg
    11701 tttcgatacg caacgccata acgttgtcgt cggtttgcgc gccggtggtt cggcggtcga
    11761 cggcggattt cgcgccgacg gcggcgcttc cgattactgc gctgacgcag ccgctaaggg
    11821 caaggctgaa aatggcggca atcagggtgc ggacggtgtg cggtttgggt ttcatcgggt
    11881 gcttcctttc ttgggcgttt cagacggcat tgctttgcgc catgccgtct gaaaagggtt
    11941 acattccttc cagcagtacg gagtcgatac agtcgcatat ggcgtgtatc agcaggatgt
    12001 ggttttcctg aatgcgggcg gtgcgcggat aggggacgtt gagcagaacg tcggtgtctt
    12061 tgagcatggc ggcgattttg ccgccgtcgc ggccggtcag ggcgatgacg tgcatatcgc
    12121 gttcgtgtgc cgctttaatg gcttcgatga cgttggcgga gttgccggaa gtggaaatgc
    12181 cgaccaagac gtcgcctgca cgtccgaggg cgcgtacctg tttgctgaag acgtggttga
    12241 agccgtagtc gttaccgatg gcggtcagcg cggatgtgtc ggtggtcagg gcgacggcgg
    12301 cgagttccat gcgctctttt tcaaagcgtc cggtcatttc ggcggcgaag tgctgggcat
    12361 cggcggccga accgccgttg ccgcacgcca gaatcttgcc gtcgttcatc aggcattgca
    12421 gcatcagctc ggcagcctgt acggtcggct cgaccagtac tttttcggct tcctgtttgg
    12481 cacggatgct ttcggcaaaa tgggcggcaa cgcgttcttg taatgtcgtc atatcgtcaa
    12541 cctgtaatat tctgtatcca ctcgggcggg cggtttcctt caatcagtac cgcatcgagg
    12601 cggcacggta cgtttgtcaa cctgttctgt tgcagataat actctacact tcgttgcagt
    12661 ttcaataatt tggatgggga aatgctgtat gcgacaccgc cgaattgccg atttttgcgg
    12721 tattttactt caacaaacag aatcataccg ccgtttttga caatcaggtc gatttcgccg
    12781 taggcgcagt gccagttgcg ggcaagcagt gtgcagcctt gggattggag gaaggcaagc
    12841 gcggcatctt cccctgcctc gccctgtttg tggtttaggc gcataatcgg tttaaccttt
    12901 ataatttata atgtttgttt ttcagacggc attttttatg tttcagaaac acttgcagaa
    12961 agcctccgac agcgtcgtcg gagggacatt atacgtggtt gccacgccca tcggcaattt
    13021 ggcggacatt accctgcgcg ctttggcggt attgcaaaag gcggacatca tctgtgccga
    13081 agacacgcgc gttaccgcgc agcttttgag cgcgtacggc attcagggca aacttgtcag
    13141 tgtgcgcgaa cacaacgaac ggcagatggc ggacaagatt gtcggctatc tttcagacgg
    13201 catggttgtg gcacaggttt ccgatgcggg tacgccggcc gtgtgcgacc cgggtgccaa
    13261 actcgccggc cgcgtgcgtg aggccgggtt taaagtcgtt cccgtcgtgg gcgcaagcgc
    13321 ggtgatggcg gctttgagcg tggccggtgt ggaaggatcc gatttttatt tcaacggttt
    13381 tgtaccgccg aaatcgggcg aacgtaggaa actgtttgcc aaatgggtgc gggcggcatt
    13441 tcctatcgtc atgtttgaaa cgccgcaccg aatcggggcg acgcttgccg atatggtgga
    13501 actgctcccc gaacgccgtc tgatgctggc gcgcgaaatt acgaaaacgt ttgaaacgtt
    13561 cttaagcggc acggttgggg aaattcagac ggcattggtg gcggacggca accaatcgcg
    13621 cggtgagatg gtgttggtgc tttatccggc gcaggatgaa aaacacgaag gcttgtctga
    13681 gaccgcgcaa aatgtgatga aaatccttgc ggccgagctg ccgaccaaac aggcggcgga
    13741 gcttgctgcc aaaatcacgg gcgagggaaa aaaagctttg tacgatctgg cactgttttg
    13801 gaaaaacaaa tagtgccgga caagccggca tcgtaagagg gaggcaatca gcgtacgcgt
    13861 cccgtcatgc ggtacagcac gtctttgcct tgaacacggt ggacgacgac catggcgacg
    13921 tgtccggtga cggcggcaaa cagcagccag cccaaattgc cgtggaacgt gttgcccaag
    13981 tttgccatcc actcgatttt ttccggcgaa ccctgcatca cttcaacgcc gaacactttc
    14041 aacgggccgc ggccgctgcc gtaatggtgg tcagaatgcc gatgaaaccg gcaacggtca
    14101 gccagtgcag cgcgcgtgtc aacgggtcgt aaacggcagg ttggtttgtg ttcataaaaa
    14161 acttattata tgtaacggtt tgaacggaca ttccgtcggg tcggaatgtc aaaaaggcgc
    14221 gattgtacca aagaagttgg acataatttt gtttgcaggc tgaagatttg cttaaaaatt
    14281 cattaagatg ggcggaacaa atagtttggg tacggcgtgt tgaaataccg cttatccctt
    14341 ataatagcgt ccgaaattct gttcggacgg catcaaaaca cacggtaatc cgtctgaacc
    14401 ccccatttga aaccaacaaa caagagcatt gaatgaaatt catcgacgaa gcaaaaatcg
    14461 aagtcgccgc aggcaaaggc ggtaatggcg caaccagttt ccgccgcgaa aaattcgtac
    14521 cgcgcggcgg cccggacggc ggcgacggcg gcaaaggcgg cagcgtttgg gcggaagccg
    14581 acgaaaacac caacaccctc gtcgaatacc gcttcgttaa acgctaccaa gccaaaaacg
    14641 gcgaaaaagg ccacggttcc gaccgttacg gcgcaggtgc ggacgacatc gtcctcaaaa
    14701 tgcccatcgg cacccttatc cgcgacctcg acaccgatga aatcgttgcc gacctcacct
    14761 accacggcca gcgcgtctgc ctcgccaaag gcggcaaagg cggcttgggc aacatccact
    14821 tcaaatcctc cgtcaaccgt gcgcccaaac aatccacgcc cggcgaagaa ggcgaaaccc
    14881 gctccctgca actcgagctt aaagtcctcg ccgatgtcgg cttattaggc atgcccaacg
    14941 ccggcaaatc caccctgatt accgccgtat ccgccgcacg ccccaaaatc gccaactacc
    15001 ccttcaccac cctgcatcca aacttaggcg tggtacgcat cgacgaaaac cacagcttcg
    15061 tgatggccga catccccggc ctgattgaag gcgcggcaga aggcgcaggc ctcggccatc
    15121 gtttcctcaa acacttatcg cgcaccggcc tgctgttgca cgtcgtcgat ttagcgccct
    15181 tcgacgaaac cgtcaacccc gccgaagaag cactcgccat catcaacgaa ttacgcaaat
    15241 acgacgaaga actctacgac aaaccacgct ggctggtgct gaacaaactt gatatgctcg
    15301 atgaagaaga agcccaagcg cgaacagccg ccttcctcga agccgtcggc tgggattacc
    15361 ccaaaccaga cgaccgcttc caattcgaca tggaaacccc gcgcctcttc caaatcagcg
    15421 cgctgaccca ccaaggcacg caggaattgg tacaccaaat caaccaatac ctgaccgaga
    15481 aaaaacgtat cgaagctgaa aaagccgaag cggagaaagc agcggcaaat gttgaaatta
    15541 tcgaacagca gcctaaaacg gatacgggcg tgtttaagcc ggagtgaaaa aagaaagggt
    15601 ttcagacgat atttgttcta caaaaggttg tttgaaacaa ttttaaagca agattgaaat
    15661 ggattaaaaa tgtcatctaa ccaatatatc cagtcgcttg agttgacaaa ctttaccgtt
    15721 cttccaaacg atacttttga attttctgaa aatctcaatg ttattgttgc cgaaaacggt
    15781 tgtggcaaaa cacatttgtt aaaaatttta tacagcctgc tagaagtaac atctaacacc
    15841 aaaaacagat tactgaaaac agaattgcaa aaaagttttg ccgataaatt actgaatgtt
    15901 ttccgtcccg attccttggg caggttgagc aaacgtttgc aggggcgcgg gcgtacggaa
    15961 atcgttttaa aactgcaaaa cggcactacg catagtagtc tgaatttttc cagtaactca
    16021 tcatcgcaag tcaatgttca aagcatcgga ttgaaagaaa atgaatatac tccgacacct
    16081 atcttcttac ccagccgcga attaattaca ctttgccctt ggttcacttc tttgtatcaa
    16141 aaccaaagca tccctttcga agaaacttgg tttgatacct gtatgcaatt aaatcacccg
    16201 cttgccaaag gccccagaga aacaaaaatt cgagaattgc tcgaacctat tgagaacgcg
    16261 atgggcggaa aagtatctga agaacaagga aggttttatc tatcgctgac aaatacgggc
    16321 ggtaagattg aagcaccttt ggtagcagag ggtttgcgga aatttgtcat gattgcccgg
    16381 ttaatcgcaa caggggcatt gctggataaa ggctatctat tttgggatga accggaagct
    16441 aatctgaatc ctaaattgat caaagtggct gcccgtatta tttggtcttt atctcaacag
    16501 ggtattcagg tttttattgc aacacactct ttattcttat tgagagaatt ggaacttttg
    16561 aagcttgaaa ataacacgga tttacctgta cgttttttct cgttgattgc ttccgatgat
    16621 ggaacaaaag tggagcaagg gaacagtatt aatgatttga acacattgat attgttagat
    16681 gaaaacatta tgcaggcaga caggtatctg aatatttgag gttaagctat gactacaatc
    16741 acagaaaaga gattaacctt tgcttttcct gaagattatt acgttaccaa gtatgacgaa
    16801 tgggaacatt ataaaatttt tcagaacagt tgtaatttac gtaataaaat tgatacaaat
    16861 gaaaagggaa agaacgggat aaatcaaagt gtagatgacg ataatggcag ctcaggtgta
    16921 gatataatag cccttcatga atcgacactt tggctaatcg aaattaaaga ttattaccga
    16981 cttgggcttg agccaaatgc ccaatcaata gatgaaaaac tttctgacct accatattta
    17041 atcgcaagaa aaattcgaga ttcattggca ggattggttt ctgctaaatt taaagcagaa
    17101 aaacaggaag aaaaagattt ttctcgcttg gctttagatt gtaacgaaat aaaaatagtc
    17161 cttcatattg aaatgcctag tataagaagt aaactttatc cctcgtcatc agatttagca
    17221 aacttattaa aagataagtt taaattatca gaatttacaa aaaattttgc aaactgttat
    17281 gcagaaccaa ttttcacaaa tatcagccat attaataatc cgcaactacg taatgttcct
    17341 tggtccgtat ctacaggaac agaacagaaa ttatcatccg agcaacaaag attaatccat
    17401 aatcccatga ccaccatcta caacacccta acccgtcaaa aagaaccctt ttcccccatc
    17461 gaccctgaaa acgtgcgtat gtacgtctgc ggtatgaccg tttacgatta ctgccatttg
    17521 ggacacgccc gcgtgatggt ggtgttcgac atgattgccc gttggttgcg cgagtgcggc
    17581 tatccgctca cttatgtacg caacatcacc gacatcgacg ataaaatcat tgcccgcgcg
    17641 gctgaaaacg gcgagaccat cggcaaactg actgcgcgtt tcatccaagc catgcacgaa
    17701 gatgccgatg ctttgggcgt gttgcgtccg gatattgagc cgaaggcaac ggaaaacatt
    17761 ccgcaaatga ttgccatgat tgaaaccctg attcaaaacg gcaaggcata tcctgccgca
    17821 aatggcgacg tttactacgc cgtgcgcgag tttgccgctt acggacaatt gtcgggcaaa
    17881 tcgttggacg acctgcgtgc gggcgaacgc gtggaagtgg acggtttcaa acgcgatccg
    17941 cttgattttg tgttgtggaa agccgccaaa gcaggcgaac cggcgtggga aagcccgtgg
    18001 ggcaacggcc gtccgggttg gcacattgaa tgctctgcca tgagtgaaaa cctgttcggc
    18061 gataccttcg acatccacgg cggcggcgcg gatttgcagt ttccgcacca cgaaaacgaa
    18121 attgcccaaa gcgtcggcgc gacggggcat acctgcggtc atcatcacgc gcaaacccac
    18181 cacgggcaaa gcatcgccag ccacgtcaaa tactggctgc acaacggctt tatccgcgtg
    18241 gacggcgaaa agatgtccaa atcgctgggc aacttcttca ccatccgcga agtgttgaaa
    18301 caatacgatc cggaagtcgt gcgcttcttc atcctgcgcg cccactaccg cagcccgttg
    18361 aactattccg acgcgcattt ggatgatgca aaaggcgcgt tgacgcgttt gtatacgact
    18421 ttgaaaaata cgccgcccgc cgatccgatg ccgtctgaag ccggcgatga ctacacgcgc
    18481 cgcttttatg ttgcgatgaa tgatgatttt gatacggtga aagcggtggc ggtgttgttc
    18541 gaacttgccg gcgaagtgaa caaaaccaat gacgcgcaac tggcaggccg tctgaaagcc
    18601 ttgggcggca tcatcggcct gctgcaacgc gacccgaccg agttcctgca aggcagcgcg
    18661 gcttcagacg gcctctccaa cgaagaaatc gaagacttga tcgcccgccg caaacaggcg
    18721 cgcgccgata aaaactgggc ggaatccgac cgcatccgcg accttctgaa cgaacacaaa
    18781 atcattttgg aagacaacgc cggcggcacg acttggcggc gcggttgaac tgtatcggaa
    18841 agggcaaaag taccgccttt tgctcttttt ccgttttcag acggcatttc ccgcttgaag
    18901 ccccgccgtt ttcagtaggc agggccgaat gggaaatgcc gggcggcgca ccgggtgctg
    18961 ctttgtgtat gggaacattg tgagaactta tttttattat gcttaaaatc atttccgcca
    19021 acgtcaacgg catccgctcc gcctacaaaa aaggttttta cgaatacatc gccgcatcgg
    19081 gcgcggacat tgtctgcgtg caggaactca aagcgcagga ggctgatttg tctgccgata
    19141 tgaaaaatcc gcacgggatg cacggccatt ggcattgcgc cgagaagcgc ggttacagcg
    19201 gcgtggcggt gtacagcaaa cgcaaacccg acaatgtgca aatcggtatg ggcattgaag
    19261 aattcgacag ggaagggcgg tttgtgcgtt gcgatttcgg caggttgagc gttatttcgc
    19321 tttatttgcc cagcggcagc agcgcggaag aacgccagca ggtgaaatac cgtttccttg
    19381 atgcgtttta ccctatgctc gaagcgatga aaaacgaagg acgcgacatt gtcgtttgcg
    19441 gcgactggaa catcgcccac caaaacatcg acctgaaaaa ctggaaaggc aatcagaaaa
    19501 attcgggctt cctaccggaa gaacgcgagt ggataggcaa ggttatccac aagctcggtt
    19561 ggacggatat gtggcgcacg ctttatcccg atgtgccggg ctacacgtgg tggagcaacc
    19621 gcgggcaggc gtatgcgaaa gatgtcgggt ggcgcatcga ttatcagatg gttacgcccg
    19681 aacttgccgc caaagccttg tccgcacacg tttataaaga tgaaaaattt tccgaccatg
    19741 cgccgctggt cgtggagtat gactatgctg ccgaataaag ttttgggtaa atatgattgg
    19801 aatgtggacg gaaaaacagg aataggcgcg gcatgggttg tcgctgcgtt tatcctgccc
    19861 atattggtgt gggcgatatt tatgttgtcg cggatgcaag gctggcttgc gccgaccaag
    19921 gcaaacccga tatgggcgtt ggtgtggctg ctgattagcc tgccctgcct gctgattgcg
    19981 tccaaatgtt tgggttggaa aggctggcgg ctgttggcaa acgttcttgc ctgcctcgcc
    20041 gtgtgcgctc tgttgagtgt gcctgccgcg ctgctgattg cgttcacgct gcaagacctg
    20101 ctcaaatagg cgcggacaat ggaaagtttc gagcttgagg atttgacgct gtggctggta
    20161 cgcgatgcgg atgaagcgga aatgtggata gaccgctggg cgatcagtta tcccgtcgtg
    20221 cagatgtccg aagcgtcggc cggtcaaagc ataggggaat ggcaggcagg gcttcagacg
    20281 gcatttgaac gcatacgcgg caaatacgtc gccgttgtcg cacacggtgc gggcgcagcc
    20341 gcatttttgg cgtggctgta tcaggcagac atcctgacac ggaagaaaat tgccaacatc
    20401 atccttgtac cgcagcgtcc cgatattttt cccgacgatg cggaacacac tttccaacgc
    20461 gtccgctgtc cctgccgtgc cgcattggtt gtacccgaac acggcggcgt gccgcacggt
    20521 tgggcgcaaa aacaggcgga tttgtggaac gcccgcctgt tggtttcccc gcattcgggc
    20581 agtttgaacg gcatgctcgg cggctggcag tggggtatga agctgatgca ggaaatgttg
    20641 ctggcgtgaa cgcttgcctt accggaattt cggctgtagc aggaagtccg catatcgggc
    20701 tgttttcatt gacgtgcagg cgcatcaggt tcggataaag tcgggctgct gcataccgcc
    20761 ggaaatggaa tcctttattt tttacgtcgg cagtgccggc agggcgttat ccggcagggt
    20821 tgcaaggaaa atgccgtctg aaacaggctt cagacggcat ttccattcaa acagggctca
    20881 caggctgccc aacacgatac gcaatacgcg gcgcagcggt tcggccgcgc cccacaagag
    20941 ctggtcgccg acggtgaacg cgctgatgta ttcgccgccc atgcccagtt tgcggatgcg
    21001 tccgacaggg acggacagcg tgccggtaac tttggcagga gtcagctcgt ggatgctggc
    21061 ttctttttcg ttggggatga ctttcaccca gtcgttcgcg cctgccaaaa tcgtttcgat
    21121 ttcggaaaca ggcaggtctt ttttcaactt cagagtgatg gcttggctgt ggcagcgcat
    21181 cgcgccgacg cggacgcaca ggccgtcaat cacggttgga ttgtcgctgc ggccgaggat
    21241 tttgttggtt tccacgccgc ctttccattc ttctttggac tggccgttgc ccaaatccac
    21301 gtcaatccac ggaatcaggc tgccggcgag cggtacgccg aagttggctt tcggatagtc
    21361 ttcgctgcgc aggaaatcgg atactttgcg gtcgatgtcg agaatcgagc cggcaggatc
    21421 ggcaagttcg tccgccactt gggcgtgaac tgcgcccata ccgctgatga gttcgcgcat
    21481 atttttcgcg cccgcgccgg aagcggcctg ataggtcata ctggtcgccc attcgaccaa
    21541 atcgttttgg aacaggccgc ccaaagccat caacatcagg gagacggtgc agttgccgcc
    21601 gatgtagttt ttcacgccgt ttttcaaacc gttgtcgatg acgttgcggt tgacggggtc
    21661 gaggacgata atcgcgtcgt ctttcatgcg cagcgaggat gccgcgtcaa tccagtaacc
    21721 gttccagccg ctgtcgcgca gggcttggaa gacggatttg gtgtaatcgc cgccttggca
    21781 ggtaacgatg atgtccattt ttgccagttc ggcaacgttg ttcgcgtcca ataatgtttt
    21841 agccgcctga ccgaaatcag gggctgcgcc gccgacgttg gaagtggtaa agaaaaacgc
    21901 ttcgggaatg tgggcgaagt cgttttcttc ttgcatacgc tgcatcaaaa ccgaaccgac
    21961 catgccgcgc cagccgacga aacctacttt cattgtgttg ctccttgagg aaaaatcgt
//
LOCUS       DAXHTY010000027        21984 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXHTY010000027 DAXHTY010000000
VERSION     DAXHTY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21984
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.027"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..1170)
                     /gene="hemW"
                     /locus_tag="ACFNMK_000848"
     CDS             complement(<1..1170)
                     /gene="hemW"
                     /locus_tag="ACFNMK_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC6364151.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALESAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQNRPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQCF"
     gene            complement(1227..3752)
                     /gene="ligA"
                     /locus_tag="ACFNMK_000849"
     CDS             complement(1227..3752)
                     /gene="ligA"
                     /locus_tag="ACFNMK_000849"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC6364152.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVANIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETTDKTIAESDQTPSEG
                     SSVGASGKHKKQPVKWAENILGGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            complement(3900..5186)
                     /locus_tag="ACFNMK_000850"
     CDS             complement(3900..5186)
                     /locus_tag="ACFNMK_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC6364153.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(5380..5952)
                     /gene="ampD"
                     /locus_tag="ACFNMK_000851"
     CDS             complement(5380..5952)
                     /gene="ampD"
                     /locus_tag="ACFNMK_000851"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC6364154.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRGGETVQFVSCDNMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFTEAQYRSLETLLAALCRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            6036..7031
                     /gene="mltG"
                     /locus_tag="ACFNMK_000852"
     CDS             6036..7031
                     /gene="mltG"
                     /locus_tag="ACFNMK_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC6364155.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            7091..7711
                     /gene="tmk"
                     /locus_tag="ACFNMK_000853"
     CDS             7091..7711
                     /gene="tmk"
                     /locus_tag="ACFNMK_000853"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC6364156.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            7933..9213
                     /locus_tag="ACFNMK_000854"
     CDS             7933..9213
                     /locus_tag="ACFNMK_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC6364157.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIASLEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRYTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYKTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            9449..9652
                     /locus_tag="ACFNMK_000855"
     CDS             9449..9652
                     /locus_tag="ACFNMK_000855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364158.1"
                     /translation="MKEMKPVRYHIGDMPETSKQTASRHDDRAVGVDDDCFHDSFGQF
                     LFGCSAEATRTAPISVKRSCLFT"
     gene            9760..10794
                     /gene="lpxK"
                     /locus_tag="ACFNMK_000856"
     CDS             9760..10794
                     /gene="lpxK"
                     /locus_tag="ACFNMK_000856"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC6364159.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNEKSRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADVSFMPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            11000..11581
                     /locus_tag="ACFNMK_000857"
     CDS             11000..11581
                     /locus_tag="ACFNMK_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC6364160.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            11650..11832
                     /locus_tag="ACFNMK_000858"
     CDS             11650..11832
                     /locus_tag="ACFNMK_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC6364161.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            11829..12590
                     /gene="kdsB"
                     /locus_tag="ACFNMK_000859"
     CDS             11829..12590
                     /gene="kdsB"
                     /locus_tag="ACFNMK_000859"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC6364162.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTI"
     gene            12613..12930
                     /locus_tag="ACFNMK_000860"
                     /pseudo
     CDS             12613..12930
                     /locus_tag="ACFNMK_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            13032..13232
                     /locus_tag="ACFNMK_000861"
                     /pseudo
     CDS             13032..13232
                     /locus_tag="ACFNMK_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            13389..13481
                     /locus_tag="ACFNMK_000862"
     tRNA            13389..13481
                     /locus_tag="ACFNMK_000862"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13423..13425,aa:Ser,seq:gct)
     gene            13907..14692
                     /gene="trpA"
                     /locus_tag="ACFNMK_000863"
     CDS             13907..14692
                     /gene="trpA"
                     /locus_tag="ACFNMK_000863"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC6364163.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNTGNEAAAVGALVKELKDAVR"
     gene            14730..15602
                     /gene="accD"
                     /locus_tag="ACFNMK_000864"
     CDS             14730..15602
                     /gene="accD"
                     /locus_tag="ACFNMK_000864"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC6364164.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEIAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPESFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(15662..16273)
                     /locus_tag="ACFNMK_000865"
     CDS             complement(15662..16273)
                     /locus_tag="ACFNMK_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC6364165.1"
                     /translation="MRRTALLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            16382..16606
                     /locus_tag="ACFNMK_000866"
     CDS             16382..16606
                     /locus_tag="ACFNMK_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC6364166.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            16607..17212
                     /locus_tag="ACFNMK_000867"
     CDS             16607..17212
                     /locus_tag="ACFNMK_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364167.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            17272..18306
                     /gene="pyrC"
                     /locus_tag="ACFNMK_000868"
     CDS             17272..18306
                     /gene="pyrC"
                     /locus_tag="ACFNMK_000868"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC6364168.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASIPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(18672..19097)
                     /gene="nusB"
                     /locus_tag="ACFNMK_000869"
     CDS             complement(18672..19097)
                     /gene="nusB"
                     /locus_tag="ACFNMK_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC6364169.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(19175..19651)
                     /gene="ribH"
                     /locus_tag="ACFNMK_000870"
     CDS             complement(19175..19651)
                     /gene="ribH"
                     /locus_tag="ACFNMK_000870"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC6364170.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(19699..20022)
                     /locus_tag="ACFNMK_000871"
     CDS             complement(19699..20022)
                     /locus_tag="ACFNMK_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC6364171.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            20189..20908
                     /gene="rnc"
                     /locus_tag="ACFNMK_000872"
     CDS             20189..20908
                     /gene="rnc"
                     /locus_tag="ACFNMK_000872"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC6364172.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            20955..21878
                     /gene="era"
                     /locus_tag="ACFNMK_000873"
     CDS             20955..21878
                     /gene="era"
                     /locus_tag="ACFNMK_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC6364173.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         4879 a         5361 c         6483 g         5261 t
ORIGIN      
        1 aaaacattgc agcaaatcgt ttaaaaacaa gcgtcctttt tcggtcgggc ggaatacggc
       61 ggggtcggtt tccagcaggc ctttttgcct tgctgtttcg atttgcgcca tgattttggc
      121 actcggtacg cccgtgcgtt cctgcaacat cgcggcgggt acgccgtcgg tcaggcgcag
      181 ggcgttcatc atgaactcaa acggcaaatc ttcggcggcg acggttttgc gttcgacggc
      241 ttcgctcggt cggttttgca ttaaggcgag gtagtcgttg gggtggcggc ggcggacggt
      301 gcgctcgatg cggtcgggat aggaaatttt gccgtgcgcg cccgcgccta tgcctaaata
      361 atcgccgaac tgccagtagt tcaaattgtg gcggcactgc atggctggtt tcgcaaaagc
      421 cgatgtttcg tagtggacaa aacccgcgct ttccagcgcg ccgtgtaccg cgtcttcgat
      481 gtcgagggcg gcttcgtctt gcggcaaacc tttcggcggc gtatgaccga acggcgtgtt
      541 cggttccatc gtcagatgat acgcgctgat gtgggtcgcg cccgtagcga tagcggtttg
      601 tacgtcgtcc aatgccgtct gaacggtttg gttcggcagg gcatacatca agtcgatatt
      661 gactttatca aataatttca aggcggtatc gatagcggtt aaggcttcct tgccgttgtg
      721 gacgcgcccc agccttgaga gcatatcgtc gttgaaactc tgtacaccaa tagaaagccg
      781 cgtgatgcct gcgtctttaa atccttgaaa cttctcgatt tcaaatgtcc ccggattggc
      841 ttccaaggta atttccgctt ccggctgcaa gcgcaacagc gaacgcacgc cgctcaataa
      901 acggtcaatc gattccgcct gaaacaggct gggcgtaccg ccgccgaaaa agatcgtttc
      961 caccggcctg ccccaaatat tgggcaattc gagctgcaag tcggtcagca gcgcgtcgat
     1021 ataggcggct tcgggcaatc cgtttttcag gctgtgggaa ttaaagtcgc aatacgggca
     1081 ttttttgatg caccacggga tgtggatgta aagcgacagg ggcggcaggg cggtgagtcg
     1141 ggtgcggttt ggaaaggaaa tggtgtgcat ggtgtggttc ggaaaagtgg acaatgccgt
     1201 ctgaaggcgg ttcagacggc ataggttcag ccgagcaggg taagcagttc ggcttcgctg
     1261 aggacggaaa cgcccaaggc attggctttt tccagcttgc tgcccgcggc ttctccggcg
     1321 acgacgtagt cggttttttt ggacacgctg ccggaaactt tgccgcctgc ggcttcgatt
     1381 agggattggg cttggtcgcg tttgagcgtg ggcagggtgc cggttaatac aaaggttttg
     1441 cctgctacgg ctttattgat gccgtctgaa ccttgcgccg cctcgtcttc agacggcatt
     1501 tgcgcgaaga aggttttcag gttttcgagt agggcggcgt tttgtggttc gctgcgccat
     1561 gcttgccagt cggcggggag ggctttgtcg ttttgtagac cttctatgtt tttaccagcg
     1621 agttcccata aggcttgggc tttgttttcg ctaattttga agtcgggcag gcgggcgatc
     1681 cagcgttgcg gttcggcgtg gcgggcgggc gggatggtaa cggcttgggt ttgcggggca
     1741 acgcctgcgg cgagcagttc gtctatcatc gcctgctgtt cggcttgggc gaagaagtgg
     1801 gcaatggaat gcgccactac cgtaccgatg tcgggcaggc aggcgaggac gggttcgggg
     1861 gcgcggcgga cgcgttccaa tgtgccgaat gcctgcgcca gcgttttggc ggtgcgttcg
     1921 ccgacgtggc ggatgccgag cgcgaacagg aagcgcgcga gttcgggcgt tttgccggat
     1981 tctatgcccc cgaggatgtt ttccgcccac ttgaccggtt gttttttatg tttgcccgac
     2041 gcgccgaccg aactgccttc agacggcgtt tgatccgatt cggcaatggt tttgtccgtc
     2101 gtttctttca ttttttgcag ggtcggaata tcgaggcggt agaggtcggc gaagtggcgg
     2161 acgaggtctt gggcgacaag ctgttcgatt tgtttttcac ccaagccgtc gatgtccatg
     2221 gctttgcgcg aggcgaagtg gattaagcct tgcgcgcgtt gtgcctgaca aagcataccg
     2281 ccgctgcatc gggcgacggc ttcgccttct tcgcgttcga tttcgctgcg gcagatgggg
     2341 caatgggtcg gcaggcggta gggcttgtgg agcggaacgg attgggtttg attagcggac
     2401 ggtgtttcgg caaacaaatc gtcctgccga tgcccgatgc cgtctgaaac agcaacggcg
     2461 gtttcctgca tcgggcggcg ttcaaaaatc acacgcacga cttcgggaat cacgtctcca
     2521 gcgcggcgca cgacgacggt atcgccgacg cgcacgtctt ttcgggaaac ttcgtcctga
     2581 ttgtgcaggg tggcgttggt aacggttacg ccgccgacga atacgggttg taatcgggca
     2641 accggcgtta ccgcgcccgt cctgccgatt tgcacgtcaa tcgcttcgac aatggtcagg
     2701 gcttcttcgg cagggaattt gtgggcaatc gcccagcgtg gcgcgcgtga gatgaagccg
     2761 agttcgcgct gttgcgccaa gctgttgact ttgaccacca taccgtcgat ttcgtagggc
     2821 agttcggggc gtttttgctg catttgttcg taaaacgcca atacttcgtc gatatttttg
     2881 aaacagccga aattgccatt tggcagactg aagccgagtg cttggaaata ggcgagttct
     2941 tggatgtgtt cttccgcgac gaaaccgcct tgctggcggg cgacggagta ggggaaaaag
     3001 tgcagtttgc gttgcgcagt gatgcgcgaa tcgagttggc gcaggctgcc ggcagcggcg
     3061 ttgcgcggat tggcaaaggg tttttgcccg ttttcggctt gtcttttatt gagggcggca
     3121 aaatcggctt tttgcatcag tacttcgccg cgtacttcga tgagttcggg cgtattttcg
     3181 ccgtgcagcc gcagggggat gttggcgacg gttttgatgt tttgggtaac gtcttcgccc
     3241 gttgtgccgt cgccgcgcgt tgccgcctgt accaatacgc cgtcgcggta gagcaggctg
     3301 atggcgaggc cgtcgaattt gggttcgata acgtattcgg gtttgccgcc gtccaaaccg
     3361 tcgcgcacgc gttggtcgaa agcgtacatt tcggcatggt cgaacacgcc gttttcatct
     3421 tgcggggaaa aagcgttggt cagcgacagc atcggcactt cgtggcgcac ttcggcaaat
     3481 cccgctaaag gctcgccgcc gacgcgctgg gtcggactgt cgggcagttt gagctcggga
     3541 tggtttaatt ccaacgcttc gagctcgcgg aataatttgt cgtattcggc atcgggtacg
     3601 ctgggcgcgt cgagggtgta gtattcgtag gcatagcggt tgagcaggtc ggtgaggtgg
     3661 cggatgtgtt gtgcggcaaa ttgttttata tcactatcag acggtttaag aagattggta
     3721 aagttagtgt tatgttttga gtttggattc atgagagaag gttttcagac gacctttgtc
     3781 tgatacggga tgaaacgggc aaaggtcgtc tgaaaaatga taggttgaaa acagctgaat
     3841 tttacccgaa aaaaagcgga tatgccgtaa cgacatatcc gctttgattg cattcgattt
     3901 taggagaaca ggcgcaatgc ggttttgccg cccggttcga taccgacttt gagcatctcg
     3961 gactgacgag ccaatacata agtgcgtacg tctttgagcc attgggtcga aacttcttcc
     4021 attttgtcgt tgaccagatt caggttcaac tggccggaca ggcgtaccgc caaatccata
     4081 aacaaatcgt cgaaggtttt ttcgcctgcc ggagagtgcg ggatgtcgag cagcatactg
     4141 aagcctttgt aggactggtt gtccaaaagg gcattggtaa acggctcgtt gttgagcgag
     4201 cagatggaga acatggtcga gcccgacgtg tcggtatagt ggaacgcgcc gtcgtcttcc
     4261 aggacgaaac ccacgcccgt tacggcggaa cgcagttcta cgccgctgat gctggtcggg
     4321 gaaaccaaat ggatggcgat ggtctggtcg acgcgcgcgc agaatgcgtc cagtgcggaa
     4381 gccacttcga taaaggcggc aaggtcggtg tgcagcgtct gaccgcccat gctttgtgcg
     4441 aatgcgtcca cctggcggtt gaatgcggag agttcttcct gcgaggcaag tccgttgcgg
     4501 ctgactgcct gaatacccac gataaatgcc tgatagcgga tgcccgggat gggttcggca
     4561 atctggaaat ggtcgtccat ggtgcagccg acaatctggt agcggcagcg gttggaaagg
     4621 cgcggcagtg cgtgcagttc tttggcttcg gtcagcgcga tataggagat gaagtcgaag
     4681 cgcacgtcaa accagggtaa ttcgactttt gacagttctt tgagcgtaat cagcggtttt
     4741 gcaggtgttt gcggaacggg tgcaggtttt gccggcgcgt cggcaggttt cggtgcggaa
     4801 tgtccggttt ggggttcgga aacggtgtgg gcggagttgc cgataatgcc gctttcttcc
     4861 aaggcggttt cgatttcggt tttgaacggg gaggcttttg cctgtttctg cttggcgatg
     4921 tagacggcat cctgttcttg caggttgcgc atggcggggt cttggggttt tgccgttttt
     4981 ttgaccgccg gttggggttt cggcatcatg actgacccgc cggacggttt gccgtcgcgg
     5041 acatggctgg ttttgctgtt gagcagggca tctttgtcgg agtgtccgaa ctggtcgcgc
     5101 acttttttgc ggtattggtt ttcctgatac atattgtagg cgacaacggc gaggacggcg
     5161 gcgaggaaca gtacgatgta aatcatggca atcactttaa atttcgggat gcaggatgcg
     5221 caaagtgcgg gtactgcggt taaatcgggc ttgcgtttcc ggcagtctga cggaacggtc
     5281 ggctataacg tttgaattat aacgaaaatt gcagggtctg acagcagtgt gtcgaaataa
     5341 gcggaaattt tccgaaatgc cgtctgaaat ctgtggtttt cagacggcat ttctgtccac
     5401 gggaaaccct ttcccccgta tccgccgcca gtcgaaaaaa tggccggggt cggttttgcg
     5461 gccgggcgcg atgtcttggt gccccgttac cgccgtgatg gggtagcggc ggcagagggc
     5521 tgccaacagc gtttcgagcg aacggtattg cgcttcggta aagggttcga aatcgcagcc
     5581 ttccaattcg atgccgatgg aaaatgcatt gcatttttcc ctgccgccga atgaagacac
     5641 gccggcgtgg tatgccatat tgtcgcagga aacgaactgt accgtttctc cgccgcgttt
     5701 gattaaaaaa tggctggata cgcgcaaagt gtgtatcagg ctgaagaacg gatgtccgtc
     5761 ggggtcgagc cggttggcaa acagcttttc caccgcatcc gtgccgtatt caaacggcgg
     5821 cagcgaaatg ttgtgcaaca cgatcaggga aaccgtttcc cccgtttccc tcgggctgaa
     5881 attgggcgac ggggtatggc gtatgctttg aagccagccg ttttgccagt gtgcttcggc
     5941 gtgattgtcc atgatgttct tcctgtccgg cgggtaattt gggttatact gtcgcccgaa
     6001 ttttaagacg tattccgaat gctgggaatc ctaccatgtt gagaaaattg ttgaaatggt
     6061 ctgccgtttt tttgaccgtg tcggcagccg ttttcgccgc gctgcttttc gtccctaaag
     6121 acaacggcag ggcataccga atcaagattg ccaaaaatca gggtatttcg tcggtcggca
     6181 ggaaacttgc cgaagaccgc atcgtgttca gcaggcatgt tttgacggcg gcggcctacg
     6241 ttttgggtgt gcacaacagg ctgcatacgg ggacgtacag actgccttcg gaagtgtctg
     6301 cttgggatat cttgcagaaa atgcgcggcg gcaggccgga ttccgttacc gtgcagatta
     6361 tcgaaggttc gcgtttttcg catatgagga aagtcatcga cgcaacgccc gacatcgaac
     6421 acgacaccaa aggctggagc aatgaaaaac tgatggcgga agttgcccct gatgccttca
     6481 gcggcaatcc tgaagggcag tttttccccg acagctacga aatcgatgcg ggcggcagcg
     6541 atttacggat ttaccaaacc gcctacaagg cgatgcaacg ccgcctgaat gaggcatggg
     6601 aaagcaggca ggacgggctg ccttataaaa atccttatga aatgctgatt atggcgagcc
     6661 tggtcgaaaa ggaaacaggg catgaagccg accgcgacca tgtcgcttcc gtcttcgtca
     6721 accggctgaa aatcggtatg cgcctgcaaa ccgacccgtc cgtgatttac ggcatgggtg
     6781 cggcatacaa gggcaaaatc cgtaaagccg acctgcgccg cgacacgccg tacaacacct
     6841 acacgcgcgg cggtctgccg ccaaccccga ttgcgctgcc cggcaaggcg gcactcgatg
     6901 ccgccgccca tccgtccggc gaaaaatacc tgtatttcgt gtccaaaatg gacggcacgg
     6961 gcttgagcca gttcagccat gatttgaccg aacacaatgc cgccgtccgc aaatatattt
     7021 tgaaaaaata aaccatgccg tctgaaaagt ttgtgttttc ggacggcata cccttaccgg
     7081 aactgcaagc atgaaaccgc aattcatcac tttggacggc atagacggtg ccggcaaatc
     7141 caccaacctt gccgtcatca aggcatggtt tgaacggagg gggctgcccg tgctgttcac
     7201 gcgcgagccg ggcggaacgc cggtcggtga ggccttgcgc gaaatcctgc tcaaccctga
     7261 aaccaaagcc ggtttgcgtg cggaaacact gatgatgttc gccgccagaa tgcagcatat
     7321 tgaagacgtg atattgcctg cactttcaga cggcatccat gtcgtgtccg accgttttac
     7381 cgatgcaacc ttcgcctatc agggcggcgg gcggggaatg ccgtctgaag acattgaaat
     7441 tttggaacat tgggtgcagg gcggtttgcg ccccgatttg accctgctgt tggatgtgcc
     7501 gctggaagtg tcgatggcgc gtatcgggca gacgcgcgag aaagaccggt tcgagcagga
     7561 acaggcagat ttcttcatgc gtgtgcgcag cgtttatctg aaccgtgccg ccgcctgtcc
     7621 cgaacggtac gccattatcg acagtaaccg cagcttggat gaagtcagaa acagcataga
     7681 aaaagtgttg gacggacatt tcggctgttg atgcggcaaa tattgaaaca agcgcatccg
     7741 cccgcgccga aaatcaaacg gcagtgccac aggtgaaaat ggcggtatgc gccaaacttt
     7801 cggcatgata gaattacgct tagttacaag gcaggatgcg tcggcaatat taacgaaccg
     7861 cccgtaaccc aatgtctcga aagtgtttcg gacagtccga ttcaaatctt tttctcgcaa
     7921 caggattgac acatggaaaa ctcattgaaa gaagccgccc tcaagttcca cgaattaccc
     7981 gttccgggca aaatttccgt taccccgacc aaatctctgg cgaccgacaa agatttggcg
     8041 ttggcgtact ctccgggcgt agccgctcct tgtatggaaa tccatgccga tccgcaaaat
     8101 gcctacaaat acaccgccaa aggcaacttg gtcgccgtca tttccaacgg taccgcagtg
     8161 ttgggtttgg gcaacatcgg cgcattggcc ggtaaacctg taatggaagg caaaggcgta
     8221 ttgttcaaaa aattcgccgg cgttgacgta ttcgatatcg aaatcgatga aaaagacccg
     8281 caaaaattgg tagacatcat cgcttctttg gaaccgactt tcggcggcat caacctcgaa
     8341 gacatcaaag cgcctgagtg cttctacatc gaacgcgaat tgcgcaaacg ttgcaaaatc
     8401 cctgtattcc acgacgacca acacggtacc gccatcatta ccgctgccgc cgtattgaac
     8461 gccttgcgtt ataccggccg caaaattgaa gaagcaactt tggtgtgctc cggtgcaggt
     8521 gctgcagcga ttgcttgctt gaaccaattg ctggatttgg gtctgaaacg tgaaaacgta
     8581 accgtctgcg actcaaaagg cgtgatttac aaaacccgcg aagacaaaga tcgcatggat
     8641 gagtctaaac agttctacgc cattgaagac aacggccagc gcgtattggc cgatgcggtt
     8701 aaaggcaaag acatcttctt gggtctgtcc ggcgcgaacc tgctgacccc cgaaatgttg
     8761 aacacgatga acgaaaaacc catcgtgttc gctatggcca acccgaatcc ggaaatcctg
     8821 ccgccgctgg caaaagaaac ccgtccggac gtggttatcg gtaccggccg ctcagatttc
     8881 ccgaatcaag taaacaacgt attgtgcttc ccattcatct tccgtggcgc gttggatgtc
     8941 ggcgcgacca ccatcaacga agaaatgaaa cgcgcctgcg tgtatgcttt ggcggatttg
     9001 gcgatggaag aagtgaccga agaagtggtt gccgcttacg gtaagaaatt tgaattcggc
     9061 gcggaatacc tgattccgac tccgttcgat tcccgcctgc tgccgcgcgt tgctacggct
     9121 gccgccaaag cagcgatgga aagcggcgtg gcaacccgtc cgattgcaga tttggaagct
     9181 tacgctgcca agctgagcga atggaagctg taagccgttt gcggtttaaa atgccgtctg
     9241 aactgttttc aggcggcatt ttgctgtcag attgatatag tggattaaca aaaatcagga
     9301 caaagcgacg aagccgcaga cagtacaaat agtacagaac cgattcactt ggtgtttcag
     9361 caccttagag aatcgttctc tttgagctaa ggcgagccaa cgccgtactg gtttttgtta
     9421 atccactata aatgaaagat actggaaaat gaaagagatg aaacctgtcc gttatcatat
     9481 tggcgatatg cccgagactt caaaacaaac cgcctcccgt catgacgaca gggcagtggg
     9541 tgttgacgat gattgttttc atgattcctt tggtcaattt ttgtttgggt gttcggcaga
     9601 ggcaacccga accgcgccaa tttctgtaaa gcgcagttgc ttatttacct gattggttcg
     9661 cttatcggtt tggtcttcgc gttgtttata ggtgggtctg tatcgggtac gcatgattaa
     9721 tgacccgggc tgattttgct tcgaggattt gtatcgaata tgccgaattg tttcaaattt
     9781 cataccgtta tcgaacggca ttggcaaaaa ccttatccgg ttttgtcttt tctgcttaag
     9841 ccgctctccg ggctgtttgc caaaattgcg gcaaaacggc ggacggattt tttatcggga
     9901 aaacggcaaa gcgaaaagct gcccgtgcct gtggtcgtgg tcggcaacat ccatgccggc
     9961 ggtacaggaa aaacgccgat tgttgccgcg ctggtgtcgg gtttgcagga aaaaggtgtc
    10021 aaggtcggca tcatcagccg gggctacggg cgcaagagca aggcggttca tgtattgaat
    10081 gagaagagcc gggcggaaga tgcgggcgat gagcctttgc tgctgttccg caaaaccggc
    10141 gcgccgacgg cggtgggcag cagccgtgca gaggcaggca gggcgttgct ggcggcgcat
    10201 cccgacatcg gactgattgt ggcggacgac ggtttgcagc attacgccct gcggcgagat
    10261 gtggaaatcg cggtgtttcc ggcggcggat acggggcgca cggatttgga tttactgccc
    10321 aacggcagtt tgcgcgaacc tttgttgcgg ctggattcgg tggatgcggt cgtcgtcagc
    10381 ggcggcaagg cggatgtgtc gtttatgccg tctgaaaata tgtttcacag ccgtatcgaa
    10441 gcgggacgga tttaccgttt gaacaatccg tccgaaatac tggacacagg ccgtctgaaa
    10501 aatcaaaccg tcgtcgccgt ggcaggtatt gccaagccgg cgcgtttttt cgattcgttg
    10561 cggaatatgg gcattaccgt gaagcgaacc gtcgcgctgc ctgaccacgc cgacatttcg
    10621 gcggcagatt tgcccgatgc ggacgcggtc attattacgg agaaagatgc ggtcaaattt
    10681 tcagacggca tttgcaccga taatgtttgg gtgttgcccg tttgtgcgat aatcgaacct
    10741 gatttggcgg cgtttgtgtt ggagcggttg gaagatgtac cgaaggccgt ctgaaagcac
    10801 ggtttgggcg gagtgattac ggatttgaat aagaacgcct cgcgccgtca ttcccgcgca
    10861 ggcgggaatc taagtctcga attttcagga atgcctagga ggcttcagaa atcccaaatc
    10921 tccggatttc cacttggaca ggaatgagaa aatcggtcgt attttttatc tgcattaatc
    10981 attcgttaaa gtattgaata ttaaactgaa aaccttgtta ttgcccttcg ccacgctggc
    11041 attgtgcacc aacgcttttg ccgccccgcc cagcgacgcg tcgttggcgc gttggctgga
    11101 tacgcagaat tttgaccggg atatagaaaa aaatatgatt gagggcttta atgccggatt
    11161 taaaccgtat gcggacaaag cccttgccga aatgccggaa gcgaaaaaag atcaggcggc
    11221 aaaagccttt aaccgctatc gtgagaatgt tttgaaagat ttgattacgc ccgaagtgaa
    11281 acaggctgtc cgcaatactt tattgaagaa tgcccgtgag atatacacgc aagaagaaat
    11341 tgacggcatg attgcctttt acggttcgcc tgtcggtcag tccgtcgttg ccaaaaatcc
    11401 gcgcttaatc aagaaatcga tgagtgaaat agcagtatct tggactgcat tgtcagggaa
    11461 aatcgcgcga catcatctgc ccgcgtttac ggaagagttg cggcgcatca tctgcggcgg
    11521 taaaaatccc gatgcgggct gtaaacaagc cggacaggtt gggaaaaggc atcagaaata
    11581 aatgatagcc gtctgaaata ttgaagaggg catccgattg attgaaccat caaacccgaa
    11641 agcaacccta tggaaaaaaa attcttagac atcctcgtct gccccgttac caaaggcagg
    11701 ctggaatatc atcaggacaa acaggaattg tggagccgtc aggcgaagct tgcctatccg
    11761 attaaagacg gcattcccta tatgctggaa aacgaagcgc gtccgttggg cgaagaggaa
    11821 ctcgaagcat gaccgaattc gtcgtattga ttccggcgcg gctggattcg tcgcgcctgc
    11881 ccggaaaagc cttggcggac atccacggca aaccgatggt cgtgcgcgtt gccgaacagg
    11941 cggcaaaaag taaagctgtg cgcgtcgtcg ttgccaccga ccatcccgat attcagacgg
    12001 cctgtcaggc gcacggcgtc gaagtcgtca tgacttcaaa ccggcacgaa agcggcacga
    12061 cgcgccttgc cgaagcctct gccgcgctga agctgccgcc gcatctggtg gttgtcaacg
    12121 tacagggcga cgagccgctg attgcacctg aactcatcga ccgcaccgcc gaagtactcg
    12181 tcgaaaacaa cgtccaaatg gcgaccgccg cccacgaatt gcacgatttc gacgaattga
    12241 tgaatcccaa tgccgtcaaa gttgtcctcg acaaaaaccg caacgccatc tacttcagcc
    12301 gcgccccgat tccctatccg cgcgatgcga tgcgtgcagg aaaacgcgaa atgccgtttg
    12361 aaaccgccgt cctgcgccat atcggcatct acgcctaccg cgccggcttc ctgcaacgct
    12421 atgccgaaat gagcgtttcg ccgctggaaa ccatcgaatc gctggaacag ctgcgcgtcc
    12481 tgtggcacgg ctacccgatt gccgtcgaaa ccgccaaaga agcccccgcc gccggtgtgg
    12541 atacgcagga agatttggat agggttcgcg ctgtatttca gactatataa aatagattca
    12601 aagggaaaag atatgcagca acatattgaa aagtggcaat acttgagccg ggaagaacag
    12661 aaaatccttg ccgaagtatg gggtctcgtg caaaacgatg atcaggaggt tcactatgaa
    12721 atgcttaaat tgaacgcacc cgacgaagcc agtggcgaat tttggttcag aatggcagaa
    12781 acactcagca ccctgccgcc caaccgttcc ctcgacctta gaatgaacgg cggcaggctg
    12841 gcgaccgccg tatccatcct ttccgtcatg attgaagaca atcccgacat accgcagctt
    12901 tgggcgcaaa aaattaccgc cctcaattat agtggattaa atttaaacca gtacggcgtt
    12961 gcctcgcctt gtcgtactat ctgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
    13021 ttaatccact atatttggca cacggacaca aagcccgtgc cgacggtttg gcacaacagc
    13081 ccgacaaagc ggcagaagcc aacgaggagg aatacctgac caaagccctg tcgcaaaacc
    13141 tgctgtcaac attggatgtc gcgcttgcac gttttcctga agacgcgtgg tttcaggaaa
    13201 tcaaacagga tgcacaaaag cattttgctt gaggatgtgg cagtcaggaa tatttccatt
    13261 caggaagaaa agaagtgcct gattgggtat aatcagggta aatcttattt tatttcaaaa
    13321 gattaatatt tgctttccat ttttccttga cggtatcgga aaagttgatt atagttacag
    13381 cttccttagg agtaatggct gagaggctga aggcacttcc ctgctaagga agcatgtggg
    13441 gtcaacctgc atcgagggtt cgaatccctc ttactccgcc agataaaaaa tagacgctgt
    13501 gttttacagc gtctattttt ttatgcaatt ttatagcggg ttggtgcaaa accagtatgg
    13561 tattgccctg tcttgattct gaattttgtt atagtggatt aacaaaaacc agtacggcgt
    13621 tgcctcgcct tagctcaaag agaacgattc tctaaggtgc tggagcacca agtgaatcgg
    13681 ctccgtacta tttgtactgt ctgcggcttc gccgccttgt cctgattttt gttaatccac
    13741 tataatccga gatgcttgcc gtttatttcc gcctcgttca aacggcggct ctgatttgcg
    13801 tggtttctgt ttgccgtatt cgcctatccg taccgcaaat gttatactgg gaaaaattta
    13861 ctgattgtgt tttatggcat atttgccgat aggatggaag agacaaatga gcagaatccg
    13921 gcaggctttt gccgctttgg atggcggaaa ggcattgatt ccctatatta cggtgggcga
    13981 ccccgatatt cggacaactt tggcattgat gcacggcatg gttgcaaacg gtgcggatat
    14041 tttggagttg ggcgtgccgt tttccgatcc gatggcggat gggccggtta ttcagcgtgc
    14101 ggcggagcgg gcgttggcaa acgggatttc gctgcgcgat gtcttggatg tcgtcagaaa
    14161 attccgtgaa accgacacgc aaacgccggt tgttttgatg ggatatttga accctgtaca
    14221 taagatgggt tatcgggagt ttgctcagga agccgcaaag gcgggtgtgg acggcgtgtt
    14281 gacggtggat tcccctgtcg aaaccatcga tccgctctat cgcgagctga aggataacgg
    14341 ggtcgactgt attttcctga ttgcgccgac gacgacggaa gaccgtatta aaaccattgc
    14401 cgagctggca ggcggatttg tctattatgt ttcgctcaag ggcgtaacgg gcgcggcaag
    14461 tttggatacg gatgaggttt cgcgtaaaat agagtatttg catcagtata tcgatattcc
    14521 catcggtgtc ggtttcggca tcagcaatgc ggaaagtgca cgcaaaatcg gccgggttgc
    14581 cgacgcagtt attgtcggca gccggattgt gaaagaaatc gaaaacaata caggcaacga
    14641 ggctgccgcc gtcggtgctt tggtaaaaga gttgaaggat gccgtgcgct gacggcggtt
    14701 cctcatcctg aatattttag gagttgtcca tgagctggtt agataaaatc ctgccaccca
    14761 aaatcaagaa tcgcggaaaa gacggttctt ccaatgttcc cgagggtcta tggcacaaat
    14821 gcccgtcttg ttcggcaacc gtttattcta ccgagttgca gcagaacaat caggtttgtc
    14881 cgaaatgcaa ccaccacaat ccgttgtcgg cacgacaacg cctgaatctg cttttggatg
    14941 aggatggcag agaggagatt gccggaaatg tcaaaccgac cgatcctttg aagtttaaag
    15001 acagcaaaaa atatccggat cgtttgagtg cggcacgcaa gctgaccggg gaagatgatg
    15061 ctttggtggt aatgaaaggc aagatgaacg gtctgcccgt cgtcgttgcc gcgtttgaat
    15121 tccgctttat cggcggttcg atgggttcgg ttgtgggcga acgattcgta caaggtatcc
    15181 gtcgggcggt tgccgacaat tgtccgttcg tctgtgtggc ggcttccggc ggtgcgcgta
    15241 tgcaggaggg tgtgaactca ttgatgcaga tgacgaaaac cagtgccgcg ctgcatttgc
    15301 tgacggaaaa acgcctgcca tttatatcgg tgttgaccga tccgactatg ggcggcgtat
    15361 ccgccagctt cgcatttttg ggcgatgtcg tgcttgccga accgaacgcg ctgatcggtt
    15421 ttgccggtcc gcgcgtgatt gagcagacgg tgcgcgaaac gctgccggaa agcttccaac
    15481 gcgccgagtt cctgctggaa aaaggcgcaa tcgaccagat tgtcgaccgc cgcgatatga
    15541 agcggcgcat cagtgatttg attacgctgt tgtgccgtca ggacaaagtt tccgccgctt
    15601 gatggctgat gaatcgaata ccgtctgaaa ccgatgtttc agacggtatt tttgtgtctg
    15661 gttatttgtt gtgcggcttt atcgacgggg catagcgtcc ggcacgttct ttcaggcgtt
    15721 gtaccaaacc tttcgtgtcg gcgggtacac cgccctcgca gaatgcctga tacaggacgg
    15781 tgcgcagtgc gtcgttgcgg cttaatgtac cgcctatcgg tttccattcg gcgtttcggg
    15841 gctgtatcca gcggcggttg accgtgtcgc cgtagccgaa cactttatag gaggaaggtt
    15901 tgccgttgcc gaacctgatg gagaagcggg cgcagaatat gccttcggca gtcaggttgt
    15961 cgtagccttt gtcggagcgg atattgagaa tgtagcggat gctgccgtcg ggcgcgggca
    16021 taatttgcag gctgtcgagc aggattttcg gctgtttgcc gtaattttca tccacataaa
    16081 tgtcgaacca gccgtccgag tgcgtatcgg gcagcggcgg cagtttggcg gtatgttctt
    16141 taaattcgcg ggcggcggcc tcttcgggcg tttcgcggta gcgggtgttg atcggcgtgt
    16201 ctttttggct gaagccggca gcgagggacg tgccggcggc gagtgcgagc agcaggaggg
    16261 cggtgcggcg cataagtttc tccaaattga aaacggcgtt attttatggg ttggcaaagg
    16321 gggctgcaag caactggggt ataatctccc cccgattccc attttttaaa cggtacaaac
    16381 gatgaacagc gaaactttag acgtaaccgg attgaaatgt cccctgccga ttttgcgggc
    16441 gaaaaaggct ttggcgcaaa tgcagcaggg cgacgtgttg accgttctgg caaccgacgg
    16501 cggcgcaccg ggggattttg aggctttttg ccgccaaacc ggtcatgtgc tgttggatgc
    16561 ttccgaacag gacggcgtgt tcacgctggt cgtccaacac aaataaatgc cgtctgaaat
    16621 gcggatgtcc cgattgatgt tattgctgtc ggcatttgtg cttgctgcgt gtaatgccca
    16681 aaaccggcaa tccggtgtac ggcatcagga tttcgcgcgt gtagccgtcg atttgtttcg
    16741 gagtgcctgc gtcctgactc aaggcagttt tgaatcggtt tccaagtttg ccgctgtcgg
    16801 acattttgtc cctgtcggaa aggaagacct gtcccatctg ccgcccactg ttgcagaacc
    16861 cgatgtacag gcgttgtgga cgcttgaacg cgatggcggc atttattatt tgagcctgac
    16921 gcgcgacagt tgcagtgtga aagccgagcg ggcagacagt gccgcgctgt tggaacattt
    16981 tgacggactt gtccgacagc cgccgaaaaa tgccaatata gaattgaggg cggaacagtc
    17041 ggtgcaggca tcatttgaaa tccgccaagt cagctatgcc tggcgggaag cgggcagctc
    17101 cgaagaaacc gtattgaccg cacaaaccgc cgattccccg gatttacccg tacaggcagt
    17161 tttgaacctg acgcaccgtt cccacaacgg gaaaccccta attctcccct aacttcagac
    17221 ggcataaagt cggcatgccg tctgaacatt caaataaaac aaaggaaaac catgcaaacc
    17281 ctgaccatta tccgccccga cgatatgcac ctgcacctgc gcgacggcga cgcgctcaaa
    17341 gccgttgctc cttataccgc ccgccaaatg gggcgtgccg tcattatgcc caacctcaaa
    17401 ccgcctgtcg tcagcgttgc cgacgcgctt gcctacaaag cgcgcatcat ggcggcattg
    17461 cccgaaggca gcgcgtttga gccgttgatg acgctttacc tgaccgacca agccacgcct
    17521 gcacttgtgc gcgaagccaa agccgccggc atcgtcgcct tcaaactcta ccctgccggc
    17581 gcgaccacca attccgattc cggcgtaacc gacctgttca agctcatccc cgtgttggaa
    17641 gaaatggcga aacagggcat tttgttcctc gttcacggcg aagtaaccga ccccgaaatc
    17701 gacatcttcg accgcgaagc cgcctttatc gggcgcgtga tgaaacccgt tttggcgcaa
    17761 gtgccgaatc ttaaagtcgt gttcgaacac atcaccaccg ccgaagccgc ccgcctggtt
    17821 ttggaagcag gcgacaacgt agccgccacc gttaccccgc aacacctcct gctcaaccgc
    17881 aacgacctct tggtcggcgg cgtgcgcccc catcatttct gcctgcccgt actcaaacgc
    17941 gaaacccacc gtcaggcatt ggttgccgcc gttaccggcg agaaggcgca taaattcttc
    18001 ctcggcaccg actccgcgcc gcacgccaaa tccgccaaag aaaacgcctg cggttgcgcc
    18061 ggtatgttca gcgcgatgac cgccatcgag ctttatgccg aagtgtttga aaaagcaggc
    18121 gcgttggaca aactcgaagc cttcgcctcg aaaaacggcg caaggtttta cggcattcct
    18181 gaaaataccg acacgatcac cctcgtcaaa caaagccaaa ccgtccccgc aagtattccc
    18241 tacggcgacg gcgaacttgt cccgatgcgc gcgggcggcg aaatcggctg gacggtacag
    18301 tattgatggg gctggaaaca aaatgccgtc tgaaaggttt ttcagacggc atttgtgttg
    18361 ttgactgatt gatacgtcaa cggcgtttga gtttgttacg tttcggttat ttccgataaa
    18421 ttcccacaat tttcaaattt cgccattccc acgaaggcag gaatccagaa attcgatgcg
    18481 accaaagttt atcaaaaacg gcagcaactc aaaaaaccgg attcccgcct gcgcgggaat
    18541 gacgagattg aagtttcaga atttatttga aatacccaaa attcaaaaaa ccaaattccc
    18601 acctgcgtgg gaatgacgaa acaaagaaag cagaaataag gacatagaac tttctttaaa
    18661 tttgtgatgc atcaacggcg tttgggctcg tcggggcgga tttgggcggc gagtttgtcg
    18721 aggatgccgt tgacgaattt gtgcccgtcc gtgccgccga aggttttggt aacttcgatg
    18781 gcttcgttga tgatgacggg gtagggtgtt tcgggcatgg cggacagctc gtggcaggcg
    18841 gtcagcagga cggcgcgttc gatgggattg aggtcttttt cgtcgcggtc gagcaggggg
    18901 cggatttttt ggatgtagtc cgctgcgttg gtttgcgtac cgaagaaaag tttgttgaac
    18961 aattcttcgt ccgctttggc aaagtcgggc atttcgcgga tgtttttagc aatttcgggc
    19021 gcggcggtgc ggttgataag ggattggtaa acggcttgta cggcaagctc gcgggaacgg
    19081 cggcgggctg ttttcatgat ttttccttga aacggttggg cggcacggta tgccgtctga
    19141 aacggaaagg gcgtattggt gtacgccctg tttgttattc ttcgtcttca aactgttctt
    19201 cgagcagcag gttgacgagg ttggcgcatt cgacggcgac tttggcggca tccgaggctt
    19261 tttcttcaat ccgttcgatt gcctgcgcgt cgttttcggt ggttaggacg gcattggcaa
    19321 tcgggatgtt gtagtcgagt gcgacgcggc taacccctgc tccggattcg ttggaaacca
    19381 gctcgaaatg gtaggtttcg ccacggatga cgacaccgat ggcaatcagt gcgtcaaact
    19441 tttcggaaga ggcaaggttc atcagtgcga tggggatttc aagtgcgccg ggtacggtgg
    19501 cgacggtaat gttttcgtct gccacgccca attcttggag ggtgcggcag cagactttga
    19561 gcatttcgct gccgatttcg ttggtgaaac gtgcctgtac gatgccgatg cggaggtgtt
    19621 tgccgtcgag gttgggggcg atggtgttca ttgggtgtcc tttggtattc ggaggtttcg
    19681 gaatgccgtc tgaaggtttc agtcttgcgg ctgccagtcg gcgacggttt ggaatgtgcc
    19741 gtcttcggca agctcccatg cgctgccttc gggttgggaa agcagtgcgg cggtttcagg
    19801 gttggttttg gcgatgtcgg cgaggctgac gatgctgaag ttgtcgggat cgtcggtgta
    19861 ttcgtcggtc tcgtcgccgc tgaagaaacg ccagccgctg tcgttttcaa aaacgggggc
    19921 ttcgcggtag aggaagccga cgggccggtt ttgtttggcg acggtgttgg tggcgataca
    19981 gcggtcgagt gccgaggaaa gtgcttgtgc aaatgcgttc attacgggaa tacgttgggg
    20041 gaaaacttac ggattttacc acgattcgtg cgttgtcggc agacggcggc ggtttggtgg
    20101 tacaatgtgc gccgtttgca gccttaaggt gtttctgtat ttttggagta tggaaacgca
    20161 ttcgggctgt tttttgcgga agacggtaat gaaagacgat gttttgaaac agcaggcaca
    20221 cgcggcgata cagaagagac tgggctacgc gttccgcgat atttcgcttt tgcggcaggc
    20281 tttgacgcac aggagccatc atgcgaagca caacgagcgg ttcgagtttg tcggtgattc
    20341 gattttgaat tatacggtgg cgcggatgct gtttgacacg tttccgaagt tgaccgaggg
    20401 cgagttgtcg cggttgcggg caagtctggt caatgagggc gtgctggcgg aaatggcggc
    20461 ggaaatgaat gtcggcgacg gtctgtactt gggggcgggc gagttgaaga gcggcggctt
    20521 cagacggcct tcgatactgg cggacgcgat ggaggcgatg tttgcggcgg tcagcttcga
    20581 tgccgatttc aacacggcgg aaaaggtggt gcgccatttg tttgccgacc gcgtccggtg
    20641 tgccgatttc caaaatcagg caaaagacgg caaaactgct ttgcaggagg cgttgcaggc
    20701 acgccgcttt gctttgccga aataccgtat cgaagagcaa atcggccatg ccaacgacag
    20761 tatgtttgtg gtttcctgcg atttgggcga actgggtttc gtgtgccgtg ccaaagggac
    20821 gagccgcaag gcggcggaac aggaggcggc gaaagaggct ttgaaatggc tggaagagaa
    20881 gctgccgctg aagaagaaaa agaaatgagg cggcgcgtga atatgccgtc tgaaatgtgg
    20941 atatgaaagc gaatatggat attgaaacct tccttgcagg ggaacgcgcc gccggcggat
    21001 accgttgcgg cttcgtggcg attgtcggtc gtccgaacgt gggcaaatca acgctgatga
    21061 accatctcat cggtcagaaa atcagcatca ccagcaaaaa ggcgcagacg acgcgcaacc
    21121 gcgtaacggg gatttatacc gacgataccg cgcagttcgt gtttgtcgat acgcccggct
    21181 ttcaaaccga ccaccgcaac gcgctcaacg acaggctgaa tcaaaacgtt accgaggcgc
    21241 ttggcggcgt ggatgtggtg gttttcgtcg tggaagcgat gcgctttacc gatgccgacc
    21301 gtgtcgtgtt gaaacaactg cccaagcaca cgccggtcat tttagtggtc aacaaaatcg
    21361 acaaggacaa ggcgaaagac cgttacgcgc tggaggcgtt tgttgcccaa gtgcgcgccg
    21421 aatttgaatt tgcggcggcg gaggcggtca gtgcgaaaca cggattgcgg attgccaacc
    21481 tgttggagct gctcaagccg tatctgcccg aaagcgtgcc gatgtatccc gaagatatgg
    21541 ttacggacaa atcggcgcgt tttttagcga tggaaatcgt gcgtgaaaaa ttgttccgct
    21601 atttgggcga ggaattgcct tatgcgatga acgtcgaagt ggagcagttt gaagaggaag
    21661 acggtttgaa ccgcatctat atcgccgttt tggtcgataa ggaaagccaa aaggcgattt
    21721 taatcggcaa aggcggggag cgtttgaaga aaatttccac cgaagcgcgg ttggatatgg
    21781 aaaaactgtt tgataccaaa gtatttttga aggtctgggt caaagtcaaa tccggttggg
    21841 cggacgacat ccgcttcctg cgcgagctgg gtttgtagtt tttcttgctg aactttacgc
    21901 aaatgccgtc cgaacaggtt tcagacggca ttttgtttca atcgggaata tctttgttaa
    21961 aaacgggttg atattatctg tgca
//
LOCUS       DAXHTY010000028        20868 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXHTY010000028 DAXHTY010000000
VERSION     DAXHTY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20868
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.028"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(23..714)
                     /locus_tag="ACFNMK_000874"
                     /pseudo
     CDS             complement(23..714)
                     /locus_tag="ACFNMK_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1020..1790)
                     /locus_tag="ACFNMK_000875"
     CDS             complement(1020..1790)
                     /locus_tag="ACFNMK_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364174.1"
                     /translation="MASIALYRLSFPVAAGGSGSRCTLCTKVHRESMLAIYSINILIY
                     NEKMFFRVTLYFVKNFVSFSKTRKSNEMMQKHKCLIDFILTVASQIAYADLPLSLEEL
                     LTDKGKFKLESSISYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQV
                     FDSQNVKAIQLSQAEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGA
                     ATAAGIGAATGVAAKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(1821..1979)
                     /locus_tag="ACFNMK_000876"
     CDS             complement(1821..1979)
                     /locus_tag="ACFNMK_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364175.1"
                     /translation="MKTPLPICTLSALAACALSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(1976..2818)
                     /locus_tag="ACFNMK_000877"
     CDS             complement(1976..2818)
                     /locus_tag="ACFNMK_000877"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6364176.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNGPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3053..3973
                     /gene="pyrB"
                     /locus_tag="ACFNMK_000878"
     CDS             3053..3973
                     /gene="pyrB"
                     /locus_tag="ACFNMK_000878"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC6364177.1"
                     /translation="MPNPLYKQHVISISDLTREQLELLLQTALKLKSEPHGNLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3983..4441
                     /gene="pyrI"
                     /locus_tag="ACFNMK_000879"
     CDS             3983..4441
                     /gene="pyrI"
                     /locus_tag="ACFNMK_000879"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC6364178.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4506..5171
                     /locus_tag="ACFNMK_000880"
     CDS             4506..5171
                     /locus_tag="ACFNMK_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364179.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALDLGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5234..6451)
                     /locus_tag="ACFNMK_000881"
     CDS             complement(5234..6451)
                     /locus_tag="ACFNMK_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6364180.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEMSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFDRSE"
     gene            6635..7138
                     /gene="def"
                     /locus_tag="ACFNMK_000882"
     CDS             6635..7138
                     /gene="def"
                     /locus_tag="ACFNMK_000882"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC6364181.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLIADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDTVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            7225..8151
                     /gene="fmt"
                     /locus_tag="ACFNMK_000883"
     CDS             7225..8151
                     /gene="fmt"
                     /locus_tag="ACFNMK_000883"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC6364182.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDVPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIRPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGAKL"
     gene            8230..9489
                     /gene="rsmB"
                     /locus_tag="ACFNMK_000884"
     CDS             8230..9489
                     /gene="rsmB"
                     /locus_tag="ACFNMK_000884"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC6364183.1"
                     /translation="MSMALAQKLAADSVAAVAEGRNLQDVLAEIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLGQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKVLDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9455..10054
                     /locus_tag="ACFNMK_000885"
     CDS             9455..10054
                     /locus_tag="ACFNMK_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC6364184.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10054..12174
                     /locus_tag="ACFNMK_000886"
     CDS             10054..12174
                     /locus_tag="ACFNMK_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6364185.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQQAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            12167..13444
                     /locus_tag="ACFNMK_000887"
     CDS             12167..13444
                     /locus_tag="ACFNMK_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC6364186.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKHGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13535..14722
                     /gene="dprA"
                     /locus_tag="ACFNMK_000888"
     CDS             13535..14722
                     /gene="dprA"
                     /locus_tag="ACFNMK_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC6364187.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEA
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14748..15209
                     /locus_tag="ACFNMK_000889"
     CDS             14748..15209
                     /locus_tag="ACFNMK_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC6364188.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15281..17587
                     /gene="topA"
                     /locus_tag="ACFNMK_000890"
     CDS             15281..17587
                     /gene="topA"
                     /locus_tag="ACFNMK_000890"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC6364189.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17660..18355)
                     /locus_tag="ACFNMK_000891"
     CDS             complement(17660..18355)
                     /locus_tag="ACFNMK_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364190.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFVPEMN
                     RILSDFKTTRGFK"
     gene            19045..19422
                     /locus_tag="ACFNMK_000892"
     CDS             19045..19422
                     /locus_tag="ACFNMK_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFC6364191.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19511..20080
                     /gene="rsmD"
                     /locus_tag="ACFNMK_000893"
     CDS             19511..20080
                     /gene="rsmD"
                     /locus_tag="ACFNMK_000893"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC6364192.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20203..20454
                     /locus_tag="ACFNMK_000894"
     CDS             20203..20454
                     /locus_tag="ACFNMK_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC6364193.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEYPETHDELMAKYEKIIQFK"
     gene            20551..20634
                     /locus_tag="ACFNMK_000895"
     tRNA            20551..20634
                     /locus_tag="ACFNMK_000895"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20585..20587,aa:Tyr,seq:gta)
     gene            20665..20738
                     /locus_tag="ACFNMK_000896"
     tRNA            20665..20738
                     /locus_tag="ACFNMK_000896"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20697..20699,aa:Gly,seq:tcc)
     gene            20748..20822
                     /locus_tag="ACFNMK_000897"
     tRNA            20748..20822
                     /locus_tag="ACFNMK_000897"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20780..20782,aa:Thr,seq:ggt)
BASE COUNT         5213 a         5295 c         5697 g         4663 t
ORIGIN      
        1 taaatgggca aaaatcggtt tattatcgtt tttgccgcat ttggatttgt tctaccgtaa
       61 aacgtgtttg acgaacggga ttcttattaa aaaacatctg atttctaaca aaatcagtat
      121 tttttggcac gatggctaaa atttttcctt ccatttcgcc atcacgtgtt ttccatgcgc
      181 tcaagaattg tgatttgctc attgagacgt gccccagcga tggatcagcc agcaaaacag
      241 tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat tttaaataca
      301 caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa ccttgtgctt
      361 caaaacccag ttcgggcatt atgcgttgca tatcatcaaa agaagcacgc atttgggttt
      421 tatccatttt gtctaagatt tctgcttcag aataatgtct gccataaaaa ttattcagta
      481 acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact atgccggaat
      541 ctcgccgtgc tttccaactt cgtacatgga tgttttggta agaagcgggg ggtcaacaaa
      601 cataggccaa gcaaaaacta tatttggggc gaaaccaatc aaagccgcat aatttatcaa
      661 tttataaaga ttttttatca taatatgtat acgcggaata aacgcatgaa acataaaaaa
      721 ataaaatcat atgcaatttt attgcatgaa taaatatgaa taaaatagat aatgatggga
      781 gtaaatacgc catgtatttt ggaagtttaa atttattaat aataaaataa tttatcgtag
      841 cgcaaataaa tcccaaaatt ggaacgatta gaaaaaaaat taatacaccc atcatcatgc
      901 aaactctata ttaataaata gctaactaat tctattgtgg aaattagtta gctaactaaa
      961 agttattaat gattatttcc gagaattgac tgcattgttg gcagcattgg ccccaaaacc
     1021 tagtgcatga atacccggtc tccatgccaa attcccagcc aatccgcctc cggcagcagc
     1081 agccagccct gttttgccgc tactcctgtt gccgcaccga ttcctgccgc agtagccgcg
     1141 ccttgcgcag ttcctaattt accatgatta tacaaattag caccatgata ctcccatgca
     1201 cctaatgcac cgccaaaagc agcggctgca ataatgggaa caaattcacc ttgtgtttct
     1261 ttcatttcag cctgtgataa ttgaattgct ttcacatttt ggctgtcaaa aacttggctg
     1321 tctaaatttt gcgccattac aggtgtaatc atcatagcca ttacagttgc aattttcgtt
     1381 gcgctggttt gcacataaat aggattagca aattcgcttt gattgcgttc agtgttgatg
     1441 tagctaatac tgctttctag tttgaattta cccttgtcag tcaataattc ttccaaactt
     1501 aaaggtaagt cagcataagc aatttggctg gcaacagtca aaataaaatc tattagacat
     1561 ttgtgttttt gcatcatttc gtttgatttt ctagtttttg agaatgatac aaagtttttt
     1621 acaaagtaaa gagtcactct gaaaaacatt ttttcattat aaatcaaaat attgatagaa
     1681 taaatagcga gcatcgattc acggtgtact ttagtgcaaa gcgtacaacg cgagcctgaa
     1741 ccaccagccg caacaggaaa agaaagccga tagagtgcaa tgcttgccaa cgtgcaagcg
     1801 agcttgcaag aacgcttggc tcaacgagag caggcaagac agaaagcaga aagcaggata
     1861 ggagcggtaa cgcaaaggtc tcgggctttg atttcgccgt aaaccctgct gccgccttgt
     1921 ccggaaaggg cgcaggcggc gagtgccgac agggtgcaga tggggagggg ggttttcatt
     1981 tggggtcgca acggaagtgg tatgcgcaga tttcaaaacc gtttttgaaa tacaggcggt
     2041 gcgcgtcggc acggtcgtgg ttgacgtgga cgttgaggtg gattttggtt acccctgttt
     2101 ccgcgccgat tttgcggact tcttccaaaa ggcgcgaggc gtagcctttg cggcggcttt
     2161 gcggcagggt aacgatgtca tcgatgtgga tgtggcggcc gctggcgagg gtgcaggctt
     2221 cgcggaagcc gcagacggcg acggcattgt gtttgccttc ttcaaaaata cccagcaggc
     2281 ggtagccttg ggggcgttgg actttgttga tctgttcggt aaagcggttg atgtcggtca
     2341 gggcggaacg caaaacgctc aaggctgcaa aggcggtggc ggtgtcgtcc gcgccgattt
     2401 cgcgcaaaac gtaggatgcg cccgaggcgg tctgttcctg tgctttctcg gcggcgtgtt
     2461 tttcttcgat tgcctgtgcc agcatgacgt gttcgtcggc ggggccgttt tgtccgccct
     2521 gttcgcgttc ttcgagcagg gctttgcagt cgatgacgcg caggtcgttg tcggcggcaa
     2581 agtccatcag gaagcggaac atttgggggt tgtcggtttc cagtttttta tcgacggcga
     2641 cgcagcggat gttgtccacc agtatggggc gcacccattt cgaataggaa agcctgtggt
     2701 ctttggtgaa cgaggacaga atgccgcaca ggcgttccgc ccagtcgctg ggacggaaaa
     2761 tcttgccgga actcgttgtg ccgtggatga cgacttcgta ggggttgcag actaacatgg
     2821 cggcttcctg aaaagaaatg tctggcgcga ttatacctta tgcttatgcg ggcgtgtttg
     2881 gatatgccgt ctgaaaagta cgggattcgt gcggtaaaac tttgcggcgg caaatgtgcg
     2941 ataatacgcg ccgtattgcc gcttttgcga agctgttccg caaacatacg ggcggcgtgg
     3001 acgtataacc ggatgcctgc ttggcgcggg tcttttacgg aggaggggtg aaatgcctaa
     3061 tccgctatat aaacagcatg ttatttctat ttctgatttg acgcgggaac agctcgagct
     3121 gctgcttcag acggcattga aactgaagtc ggagccgcac ggcaacctgt tggaagggaa
     3181 acttatcggt tcgtgcttct ttgaaccgtc cacgcgtacg cgcctgtcgt ttgaaacggc
     3241 ggtacagcgg ctgggcggca aggtcatcgg cttttcggac ggcgcgaata ccagtgccaa
     3301 aaaaggcgag acgcttgccg ataccgcccg catcatttcc ggctacaccg acgcaatcat
     3361 ccaacgccat cccaaagacg gcgcggcgcg cgtggcggcg gagttttcgc gcgtccccgt
     3421 tatcaacgcc ggcgacggca cgaaccagca ccccagtcag acgctgctcg acctggttac
     3481 catttatgaa acgcagggac gtttggacaa gctcaaaatc gccatggcgg gcgacttgaa
     3541 atacggacgt accgtgcatt cgctttgtca ggcgttgaaa cgctggggct gcgaatttgc
     3601 ctttgtttcg ccgcccagcc tcgcgatgcc cgactacatt accgaagagt tggacgaagc
     3661 cggctgccga taccgtatcc tcggtagttt ggaagaagcg gcggaatggg cggacatcct
     3721 gtatatgacc cgtgttcaac gcgaacgttt cgacgaacag gaatttgcca aaatccaagg
     3781 caaattcaat ctcgacgcat ccatgcttgc ccgcgccaaa ccgaacctgc gcgtgctgca
     3841 ccctttgccg cgcgtggacg aaatccatcc cgatgtcgat gctacgccgc atgcctatta
     3901 tttcgagcag gcgaccaacg gcgtttatgc gcgtatggcg atattgtcgc tggtgttgaa
     3961 cgaagaagtg tgaggaaccg atatggaaac cccgaaactc agtgtcgaag ccattgaaaa
     4021 aggtacggtt atcgaccata ttcccgccgg cagggggctg accatcctgc gccagttcaa
     4081 acttttgcac tacggcaacg cggtaaccgt gggcttcaac ctgcccagca aaacccaagg
     4141 cagcaaagac atcatcaaaa tcaaaggcgt gtgcttggac gacaaagccg ccgaccgcct
     4201 cgccctgttc gcccccgaag cggtggtcaa taccatcgac aatttcaagg tcgtgcagaa
     4261 gcggcatttg aacctgcccg atgaaattgc cgaagtgttc cgttgcccga acacgaattg
     4321 cgccggccac ggcgagccgg tcaaaagccg gttttatgtt aaaaaacaca acgggcagac
     4381 gcggctgaaa tgccattact gcgaaaaaac ctacagccgg gattcggtgg cggaagcctg
     4441 acggattccc ttaaaccgag cgggcggcat ttcgtctgcc gcttgttttg ccaatctgaa
     4501 atggaatgat gatgcacgct tctgtccaaa gccgtttcgc accgatactt tatgttttga
     4561 ttttctttgc cggttttttg accgcgcaaa tttggttcaa tcagaaagcc tatactgaag
     4621 agctgcctcc gcttctgtcc gcattgtccg ccgtcgcgct ggtgtggctg gcgtgggcgt
     4681 tcgtgtcggc gcgttcaaag gctaaggcgg aaaagttcta ccgcgaaaaa atgatacaga
     4741 acgaaagcat acaccccgtc ctgcacgctt ctttgcaaca cttggaacac aagccgcaaa
     4801 tgctcgccct gctggtcaaa aaccacggca aagggatggc ggaacaggtc aggttcaagg
     4861 cggaagtgct gcccgacgac gaagacgcgc gcacgattgc cgccgagttg gcaaaaatgg
     4921 atatgtttgc attggggacg gacgcggtcg cctcgggcga aacctatgga cgcgtgttcg
     4981 ccgatatttt cgagttgtcg gcggctttgg aagggcgcgc gttcaaagga atgttgaaac
     5041 tgacggcgga atataaaaac atcttcggcg atgcctgccg ttcggaaacg gcgttggatt
     5101 tgggcgcgct caatcaggcg ttgcaggaga tttcaaaaac atcggaaaag tccaaacgga
     5161 tattttattg aagatggaaa aatgccgtct gaaacggaag gtgtttcaga cggcattttt
     5221 gtcggatgat taattattcg gagcggtcga agccaaactt cacgcggctg cggccctgat
     5281 ccggtatatt gtccaaatcg cgtcccggat tggcggcggt gtcgcctacg gaaatatcgg
     5341 agatgttttc caaaatgatg gcggacgaca ggtgttcgga ggtgcggtaa accattgcca
     5401 agcccacttc ttcggcagga gtggaaatca gctcgacggt atccctgctt ttgaaattgt
     5461 tggagaggtc gacctgcatc gttttcttgc gtttgtagag gctcaaaacc gtgcctttgt
     5521 ccaaaccgtc cgcctcgcct ttgtcgatgg tgatggtttg aaactggccg gcaatccttg
     5581 taccttcaaa cacggaaacg attttagcct gaaccgggcg ggacggttcg tgcggcatca
     5641 tgttgaagcg gtcggtgtct tccggcattt tcatcaggta gtcgccctgc tgtatttcgg
     5701 aaatggcggt ttcgaccacc agcggctgta tcgaaggggt gcgcagcggg gtaatcaaag
     5761 gatgggtgcg ggtatggtat tcgttgtctt tcggccgttc tccagcctgt ttcgagcgtt
     5821 gttcgaggac agagtcggta tagtcgaggg agcgcacgat gccgctgaat gcgacttcct
     5881 gcccgaggaa tttacccgta tccggatcgg tgatgttttt attgattcgg taggtcaggt
     5941 agcggcccgg ctctttcagg cctttggtgt aaaccctggt gcctttggtg tacagcagcc
     6001 tgccttccgg gcccgagagc aggcgcggcg cggcagcggt ttctttgcgg gaaacgattt
     6061 gcgggtgccg cataaagatg cggtagaagt tgacatcgat ggcgggaata ccgtatccgg
     6121 acatttcctt atccggactc attttgacga cggggatgcc gtctgtctgt tccaagccga
     6181 ggcgcggttc gccgtcaacg tggcgcaaca ccaatacctg gtccggataa atcaggtcgg
     6241 gattgtggat ttgatcccgg ttcgcgtccc acaggcggcc ccattgccac gggctgtaca
     6301 ggtatttgcc cgaaatgccc cacagggtgt cgccctgttt gaccgtgtag cgttccggcg
     6361 cgttcgggcg cacctccaaa tttgccgcca aagtttgtgt tgagaatgcc atacctgccg
     6421 cgcagagcag ggttataata cgacgttgca taaccgttcc ccttatctga taaatttcgg
     6481 tttgtcttgc ttgattgggt tggaaaaagc ggcggcagcc cctcgggatg tgccgcgtga
     6541 taaaaaatgt tccgcatttt aacatcgaat tatccgcacc atcacggtaa ttatgaaaaa
     6601 caggcggcgt atccgccgaa ggaaagagaa aattatggct ttattgaata tcttgcaata
     6661 tcccgacgag cgtctgcaca cggtggcaaa gcctgtcgaa caagtcgacg agcgcatccg
     6721 gaagctgatt gccgatatgt ttgaaacgat gtacgaatcg cgcggcatcg ggctggcggc
     6781 gacgcaggtc gatgtgcacg agcgcgtggt cgtgatggat ttgaccgaag accgcagcga
     6841 accgcgcgtg ttcatcaacc ccgtcatcgt tgaaaaagac ggcgaaacca cttacgaaga
     6901 gggctgcctg tccgtgccgg gcatttacga caccgtaacc cgcgccgaac gcgtcaaggt
     6961 cgaggctttg aacgaaaaag gcgaaaagtt cacgctggag gcggacggct tgctggcgat
     7021 ttgcgtgcag cacgagttgg atcacctgat gggcatcgtg tttgtcgaac gcctgtcgca
     7081 gctcaagcag gggcggatta agaccaagct gaaaaaacgt cagaaacata cgatttgacc
     7141 cttttgccgt gccgtctgaa cgctgcaaag ttttcagacg gcacggtctt gtccgacaat
     7201 tttacgcaca cgcaggaaca cgctatgaaa gtcatcttcg ccggcacgcc cgattttgcc
     7261 gccgccgcct taagagccgt tgccgccgcc ggttttgaaa tcccgctggt gctgacccag
     7321 cccgaccgtc cgaaagggcg cggtatgcaa ctgactgccc cgcccgtcaa acaagccgcg
     7381 ctggaactcg gtttgcgcgt cgaacagccc gaaaaactgc gtaacaacgc cgaagccctg
     7441 caaatgctca aagaggtcga ggcagacgta atggtggttg ccgcctacgg tttgattctg
     7501 ccgcaagacg tgttggatgt gccgaaacac ggctgcctca atatccacgc ctcgctgctg
     7561 ccccgttggc gcggcgcggc gccgattcag cgcgcgattg aggcaggcga tgccgaaacc
     7621 ggtgtgtgca ttatgcagat ggacatcggt ttggacaccg gcgatgtggt cagcgaacac
     7681 cgctacgcca tccgacctac cgataccgcc aacgaagtcc acgacgcgct gatggaaatc
     7741 ggtgcggcgg cggttgttgc cgatttgcaa cagcttcaaa gcaaaggccg tctgaacgcg
     7801 gtcaaacagc ccgaagaagg tgttacttac gcccaaaaac taagcaaaga agaggcgcgt
     7861 atcgattgga gcgaaagcgc ggcggttatc gaacgcaaaa tccgcgcctt caaccccgtg
     7921 cctgccgcgt gggttgagta tcagggcaag ccgatgaaaa tccggcgggc ggaagtggtg
     7981 gcgcaacaag gcgcggcagg cgaagtgttg tcctgttcgg cggacggttt ggtcgttgcc
     8041 tgcggcgaaa acgcgctgaa gattaccgaa ttgcagcctg ccggcggcag gcggatgaat
     8101 atcgcggcgt ttgcggcggg acggcatatc gaagcagggg cgaagctgta aatcccttca
     8161 gacggcattc cgatccgcaa acgggaatgc cgtctgaaac catcagtcga agaaagcgaa
     8221 tcacataata tgagtatggc acttgcccaa aaacttgccg ccgacagcgt tgccgcggtt
     8281 gccgaagggc gcaatcttca ggatgttttg gcggaaatcc gcgcggcgca tccgcagctg
     8341 acggcgcagg aaaacggcgc gttgcaggat attgcctacg gttgccaacg ttatttgggc
     8401 agtttgaagc acatgcttgg tcaaatgctg aagaagccga ttgacaatcc gcagctcgaa
     8461 agcctgcttt tggcagcgtt gtaccaactg cattacacgc gcaacgcgcc gcacgccgtg
     8521 gtcaacgaag ctgttgaaag catcgccaaa atcggacgcg ggcagtaccg ttcgtttgcc
     8581 aacgcggttt tgcgccgctt tttgcgcgaa cgcgacaagc ttgcagcttc ctgtaaaaaa
     8641 gacgatgtgg cgaaacacaa cctgccgctg tggtgggtgg cttacctgaa aaaccattat
     8701 ccgaaacact ggcacaacat cactgccgcg ctgcaatccc atccgccgat gacgttgcgc
     8761 gtcaaccgcc gacacggcaa tgccgaaagc tatttggaaa aactggtggc ggaaggtatc
     8821 gcggctaagg tgttggacga atatgcggtt acgttggaag aagccgtgcc ggtaaaccgc
     8881 ctgcccggtt tttcagacgg catcgtttcg gtacaggact tcggcgcgca gcaggcggcg
     8941 tatctcctta acccgaaaga cggagaacgg attttggacg cgtgcgccgc gccgggcggc
     9001 aagacggggc atatcttgga actggcggat tgccgcgtta ccgccttgga catcgatgca
     9061 ggccgtctga agcgtgtgga ggacaatatc gcgcgcctgg gctttcagac ggcatcggcg
     9121 gcgtgtgccg atgcgcggga tttggcggcg tggtatgatg gaaagccttt cgacgcaatc
     9181 cttgccgacg tgccatgcac cgcttccggc gtggcgcggc gcaatcccga cgtgaaatgg
     9241 ctgcgccgtc cgaccgacgc gctcaaaacc gcccgccagc aggaagccct gctggacgca
     9301 ctttggcaga cgctgaaaca gggcggcaga atgctgcttg ccacctgttc cgtgttcgtc
     9361 gaagaaaacg acgggcaatt gcaaaaattc ctcaaccgcc atgccgatgc cgaaccgatc
     9421 gaatcgcggg tgcttttacc gaacaaacac caagatggct tttattacgc gcttattcaa
     9481 aagcattaaa caatggcttg tgctgctgcc gatgctttcc gttttgccgg acgcggcggc
     9541 ggaggggata gatgtgagcc gcgccgaagc gaggataacc gacggcgggc agctttccat
     9601 cagcagccgc ttccaaaccg agctgcccga ccagctccaa caggcgttgc gccggggcgt
     9661 gccgctcaac tttaccttaa gctggcagct ttccgccccg ataatcgctt cttatcggtt
     9721 taaattgggg caactgattg gcgatgacga caatattgac tacaaactga gtttccatcc
     9781 gctgaccaac cgctaccgcg ttaccgtcgg cgcgttttcg acagactacg acaccttgga
     9841 tgcggcattg cgcgcgaccg gcgcggttgc caactggaaa gtcctgaaca aaggcgcgct
     9901 gtccggtgcg gaagcagggg aaaccaaggc ggaaatccgc ctgacgctgt ccacttcaaa
     9961 actgcccaag ccttttcaaa tcaatgcatt gacttctcaa aactggcatt tggattcggg
    10021 ttggaaacct ctaaacatca tcgggaacaa ataatgcgcc gttttctacc gatcgcagcc
    10081 atatgcgccg tcgtcctgtt gtacggactg acggcggcaa ccggcagcac cagttcgctg
    10141 gcggattatt tctggtggat tgttgcgttc agcgcaatgc tgctgctggt gttgtccgcc
    10201 gttttggcac gttatgtcat attgctgttg aaagacaggc gcgacggcgt attcggttcg
    10261 cagattgcca aacgcctttc tgggatgttt acgctggttg ccgtactgcc cggcgtgttt
    10321 ctgttcggcg tttccgcaca gtttatcaac ggcacgatta attcgtggtt cggcaacgat
    10381 acccacgagg cgcttgaacg cagcctcaat ttgagcaagt ccgcattgaa tctggcggca
    10441 gacaacgccc ttggcaacgc catccccgtg cagatagacc tcatcggcgc ggcttccctg
    10501 cccggggata tgggcagggt gctggaacat tacgccggca gcggttttgc ccagcttgcc
    10561 ctgtacaatg ccgcaagcgg caaaatcgaa aaaagcatca acccgcacaa gctcgatcag
    10621 ccgtttccag gtaaggcgcg ttgggaaaaa atccaacagg cgggttcggt cagggatttg
    10681 gaaagcatag gcggcgtatt gtacgcgcag ggctggctgt cggcgggtac gcacaacggg
    10741 cgcgattacg ccttgttttt ccgtcagccg gttcccaaag gtgtggcaga ggatgccgtc
    10801 ttaatcgaaa aggcaagggc gaaatatgcc gagttgagtt acagcaaaaa aggtttgcag
    10861 acctttttcc tggcaaccct gctgattgcc tcgctgctgt cgatttttct tgcactggtc
    10921 atggcactgt atttcgcccg ccgtttcgtc gaacccgtcc tatcgcttgc cgagggggcg
    10981 aaggcggtgg cgcaaggcga tttcagccaa acccgtcctg ttttacgcaa tgacgagttc
    11041 gggcgtttga ccaagttgtt caaccacatg accgagcagc tttccatcgc caaagaagca
    11101 gacgagcgca accgccggcg cgaggaagcc gccagacatt atcttgaatg cgtgttggag
    11161 gggctgacca cgggcgtggt ggtgtttgac gaacaaggct gtctgaaaac cttcaacaaa
    11221 gcggcggaac agattttggg gatgccgctt acccccctgt ggggcagcag ccggcacggt
    11281 tggcacggcg tttcggcgca gcagtccctg cttgccgaag tgtttgccgc catcggcgca
    11341 gcggcaggta cggacaaacc ggtccatgtg aaatatgccg cgccggacga tgccaaaatc
    11401 ctgctgggca aggcaaccgt cctgcccgaa gacaacggca acggcgtggt aatggtgatt
    11461 gacgacatca ccgttttgat acacgcgcaa aaagaagccg cgtggggcga agtggcaaaa
    11521 cggctggcac acgaaatccg caatccgctc acgcccatcc agctttccgc cgaacggctg
    11581 gcgtggaaat tgggcgggaa gctggatgag caggatgcgc aaatcctgac gcgttcgacc
    11641 gacaccatcg tcaaacaggt ggcggcattg aaggaaatgg tcgaagcatt ccgcaattat
    11701 gcgcgttccc cttcgctcaa attggaaaat caggatttga acgccttaat cggcgatgtg
    11761 ttggcattgt atgaagccgg tccgtgccgg tttgcggcgg agcttgccgg cgaaccgctg
    11821 atggtggcgg cggatacgac cgccatgcgg caggtgctgc acaatatttt caaaaatgcc
    11881 gccgaagcgg cggaagaagc cgatgtaccc gaagtcaggg taaaatcgga agcggggcag
    11941 gacggacgga ttgtcctgac ggtttgcgac aacggcaaag ggttcggcag ggaaatgctg
    12001 cacaacgcct tcgagccgta tgtaacggac aaaccggcgg gaacgggatt gggactgccc
    12061 gtggtgaaaa aaatcattga agaacacggc ggccgcatca gcctgagcaa tcaggatgcg
    12121 ggtggcgcgt gtgtcagaat catcttgcca aaaacggtag aaacttatgc gtagcagcga
    12181 tattttaatt gtagacgacg aaatcggcat ccgcgacctg ctgtcggaaa tcctgcagga
    12241 cgaaggttat tcggtcgcat tggcggaaaa cgccgaagag gcgcgcaagc tgcgccatca
    12301 ggcgcgtccg gcgatggtgc tgctggatat ttggatgccc gattgcgacg gcatcaccct
    12361 tttgaaggag tgggcgaaaa acgggcagct caatatgccg gtggtgatga tgagcgggca
    12421 tgccagcatc gataccgccg tagaagcgac caaaatcggc gcgctcgact ttttggaaaa
    12481 accgatttcc ctgcaaaagc tgctgtctgc cgtcgaaaac gcgttgaaac acggtgcggc
    12541 gcaaaccgaa acggggcctg tattcgacaa gctgggcaac agtgcggcga ttcaggaaat
    12601 gaaccgtgag gtaggggctg cggtgaaacg tgcctctccc gtacttttga cgggcgaggc
    12661 aggttcgccg tttgaaacgg tggcacgcta tttccataaa aacggtacgc cgtgggtcag
    12721 cccggcaagg gtcgaatatc tgatcgatat gccgatggaa ctgttgcaga aggcggaggg
    12781 cggcgttttg tatgtcggcg acatcgccca gtacagccgc aacatccaag ccggtattgc
    12841 ctttattgtc ggcaaggcgg aacaccgccg cgtcagggtg gtcgcatcgg gcagcagggc
    12901 ggcaggttca gacggcattg cctgcgagga aaagctggcg gaactgctgt cggagtcggt
    12961 cgtccgcatt ccgccgctgc gtatgcagca tgaagatatt cccttcctga tacaggggat
    13021 tgcctgcaat gtggcggaaa gtcaaaagat tgcgcccgcc gcattcagtg aagaggcact
    13081 tgccgcattg acccgttacg actggccggg aaatttcgac caactgcaaa gcgtcgttgc
    13141 aacgctgttg ttggaggcgg acggacagga aatcggcgca ggggcggttt cttccctttt
    13201 ggggcagaat gtgcctgccg agggagcgga agatatggtg ggcgggttta atttcaacct
    13261 gcccctgcgc gaattgaggg aggaggtgga gcggcgttat ttcgagtacc acatcgccca
    13321 agaaggtcag aatatgagca aagtggcgca gaaagtcggt ttggaacgca cgcaccttta
    13381 ccgcaaactc aaacagctcg gcatcggcgt ttcgcgccgg gcgggggaaa aaaccgaaga
    13441 ataggcccgg acggccggtt ttccggctgc gggcttttgt tttcagacgg catttggtgc
    13501 aaatgccgtc tgaaatcgta aggggacgga ttttatgaca gaggacgaac gtttcgcgtg
    13561 gttgcaactg gcttttacgc cctatatcgg tgcggaaagt ttcttgctgc tgatgcgccg
    13621 tttcggcagc gcgcaaaatg ccttgtccgc accggcggaa caggtcgccc cggcggtgcg
    13681 gcacaagcag gcggccgagg cttggcgcaa tgcggaaaaa cgcgctttgg cgcggcaggc
    13741 ggcagaagcg gcgttggaat gggaaatgcg ggacggatgc cgcctgatgc tgcttcagga
    13801 tgaagatttc cccgaaatgc tgacgcaggg gctgaccgcg ccgccggttt tgtttttgcg
    13861 cggcaacgtg cggctgctgc acaagccttc cgccgccatc gtcggcagcc gccatgccac
    13921 gccgcaggcg atgcggattg ccaaagattt cggcaagtcg ttgggcgggc aaaatatccc
    13981 cgttgtgtcg ggtatggctt ccggtatcga tactgccgcc catcagggcg cactggaggc
    14041 agaaggcggc accatcgccg tgtgggggac gggtatagac cgcatttacc cgccgtccaa
    14101 taaaaacctt gcctatgaaa tcgccgaaaa aggattgatt gtcagcgagt ttcccatcgg
    14161 tacgcgcccg tatgccggca actttccgcg ccgcaaccgc ctgattgccg cgctgtcgca
    14221 ggttacgctg gtggttgaag ctgcgttgga atccggttcg ctgattaccg ccggcttggc
    14281 ggcagaaatg gggcgcgaag tgatggcagt acccggttcg atagacaatc cgcacagcaa
    14341 aggctgccac aaactgatta aagacggcgc gaaattggtg gaatgcctgg acgacatcct
    14401 gaacgaatgc ccggggctat tgcaaaatac gggtgcttca tcatattcta taaataagga
    14461 tacgcccgac acgggacgcc gcgccgttca gacggcatac gccccgccgc ctgccgcaaa
    14521 aatgccgtct gaagcggcgg caggcggcac agccgtcggc ggcatactgg ataaaatggg
    14581 tttcgacccc atccatcccg acgtgcttgc cggacagttg gctatgcctg ccgcagattt
    14641 gtatgccgca ctgttggaat tggaattgga cggcagcgtt gccgcaatgc ccggcggcag
    14701 ataccagcgt atccgaactt gaacgcactt tatattaagg aacacgaatg accgaagtca
    14761 tcgcctacct catcgaacat ttccaagatt tcgatacctg tccgccgccc gaagacttgg
    14821 gtatgctgct tgaagaagcg ggtttcgata cgatggaaat cggcaacacc ctgatgatga
    14881 tggaagtatt gctcaacagc tccgaatttt ccgccgaacc tgccgacagc ggcgcattgc
    14941 gcgtgtacag caaagaagaa accgacaacc tgccgcagga agtgatgggg ctgatgcagt
    15001 atctgattga agaaaaagcc gtcagctgcg aacagcggga aatcatcatc cacgcgctga
    15061 tgcacattcc cggcgacgaa attaccgtag ataccgccaa agtgctgacc ctgctgcttt
    15121 tatgggcaaa caagagcgag ctgcccgtgt tggtcggcga cgagctgatg agcgcgcttt
    15181 tactcgacaa caaacccacg atgaactgaa gcggcttcag acggcccgcc cgaggccgtc
    15241 tgaaacgccg gcatcaaaac caccatccag agaacgacaa atggcgaaaa acctattaat
    15301 cgtcgaatcc ccgtccaaag ccaaaaccct gaaaaaatat ttgggcggcg attttgaaat
    15361 ccttgcatcc tacggacacg tccgcgacct cgtccccaaa agcggcgcgg tcgatcccga
    15421 caacggcttt gcgatgaaat accaactcat cagccgcaac ggcaaacacg tcgatgccat
    15481 tgtcgccggt gccaaagaag ctgaaaacat ctacctcgcc accgacccgg atagggaagg
    15541 cgaagccatt tcctggcatc ttttggaaat cctcaaatcc aaacgcggct tgaaaaacat
    15601 caagccgcag cgtgtcgtgt tccacgaaat caccaaaaac gccgtgctcg atgccgttgc
    15661 ccatccgcgc gaaatcgaaa tggacttggt cgatgcgcaa caagcccgcc gtgctttgga
    15721 ctatttggtc ggctttaacc tttcgccatt gttatggaaa aaaatccgcc gcggtttgag
    15781 cgcgggtcgt gtgcaaagtc ccgctttgcg cctgatttgc gaacgcgaaa acgaaatccg
    15841 cgcgtttgaa gcgcaggaat attggacggt acatctagac agccacaaag gccgcagcaa
    15901 gttcaccgcc aaactcgccc aatacaacgg cgcgaaactc gaacaattcg acctgccgaa
    15961 cgaagccgct caagccgatg tgttgaaaga actcgaaggc aaagaggccg tcgttaccgc
    16021 catcgaaaag aaaaagcgca gccgcaatcc tgccgcgccg ttcaccactt ctaccatgca
    16081 gcaggatgcc gtgcgcaaac tcggcttcac caccgaccgc accatgcgta ccgcccaaca
    16141 gctttatgaa ggtatagacg tagggcaggg cgcgatcggt ctgattacct atatgcgtac
    16201 tgacagcgtg aatttggcgg atgaagcgtt aaccgaaatc cgccattaca tcgaaaacaa
    16261 aatcggcaaa gaatatctgc cgagtgccgc caaacaatac aaaaccaaat ccaaaaacgc
    16321 ccaagaagcg cacgaagcca tccgtccgac ttccgtgtac cgcacgcccg aaagcgtcaa
    16381 acccttcctg agcgcagacc agttcaaact ctatcaaatg atttggcagc gtaccgtcgc
    16441 ctgtcagatg acgcctgcca aattcgacca aaccaccgtc gatattaccg tcggcaaagg
    16501 cgtattccgc gtaaccggac aagtgcaaac cttcgcaggc ttcttgagcg tttatgaaga
    16561 aagcagcgac gatgaagaag gcgaagacag caaaaaactg cccgaaatga gcgaaggcga
    16621 taaattgccc gtggacaaac tctacggcga acaacacttt accactccgc cgccgcgcta
    16681 caacgaagcc acgctggtta aagccctcga agaatacggc atcggccgcc cctcgaccta
    16741 cgccagcatc atctccacgc tcaaagaccg cgaatacgtt acccttgagc aaaaacgctt
    16801 catgcccacc gacacaggcg acatcgtcaa caaattcctg accgaacact tcgcccaata
    16861 cgtcgattac cacttcaccg ccaaactcga agaccagctt gacgaaattg ccgacggcaa
    16921 acgccaatgg attcccttga tggacaaatt ctggaaaccg ttcatcaagc aagtggaaga
    16981 aaaagaaggc atcgaacgcg ccaaatttac cacgcaggaa cttgatgaaa cctgcccgaa
    17041 atgcggcgaa cacaaactgc aaatcaaatt cggcaaaatg ggtcgttttg ttgcgtgtgc
    17101 cggttatccc gagtgcagct acacgcgcaa tgtcaacgaa accgccgaag aggccgccga
    17161 gcgcatcgcc aaagccgaag ccgaacaggc cgaactcgac ggacgcgagt gcccgaaatg
    17221 tggcggtcgc ctagtgtaca aatacagccg caccggcagc aaattcatcg gctgcgccaa
    17281 ctatccgaaa tgcaaacacg tcgagccgct ggaaaaaccg aaagataccg gcgtccagtg
    17341 tccgcaatgc aaaaaaggca acctcgtcga gcgcaaatcc cgctacggca aactgtttta
    17401 cagttgcagc acctatcccg actgcaacta cgccacttgg aacccgcccg ttgccgaaga
    17461 atgcccgaac tgccattggc cggtcttgac catcaaaacc accaaacgct ggggcgtcga
    17521 aaaagtctgc ccgcaaaaag aatgcggctg gaaagaacag attgaaccgc ccgcgccgaa
    17581 ggagtaagat taggttggtt tgaaagagaa aaggtcgtct gaaaaatttt cagacgacct
    17641 ttgcttttct gtgattggtt tatttgaatc cgcgtgttgt tttaaagtcc gataaaatcc
    17701 ggttcatttc aggcacaaac aaggcgatgt aatcgtaaga tagaccgcga ctggcactgg
    17761 gatggggaaa gcagacgact tcgcaatctt caaacgattg gaatttgaca ttgaaacgtg
    17821 taccgtcaaa ttctttttgc accgtctcca gcggtttggt ctgcttaccg accaactgct
    17881 cgaagcgtgg cagtacattt tggttgttca gaaaatccgc caacctgctg cccatgaaga
    17941 ggatgacttt cggacgcagt ttttcgatgt ggtagagaaa attatcgatg tgctcgggtt
    18001 gtgtgaactt gtcgggattg tcgatagtgt tgccctgtgt agcagcccag ttggtttgaa
    18061 ccagggattt ttcaaatgca ccgcccaatc cattttcgtc taaggggtgt ccccacattt
    18121 caaaccaatt ttttatcgta ttgtcgtaac gccacttctt tgcctgctct ccgaaataga
    18181 gggatttgtt tgcaaatgta tggtcgattt tgttttcagg gagtttgtat tcacctgcta
    18241 cataagcagc ctcatcggct ttactccaac cccattcata cccacaaatc attaaaccat
    18301 gtttgtcgtt gtagcctttg aacagactgt tgctcaaatt caaatccttc atcatgaact
    18361 cttcctttta aaatttaaga gcgattgact tcaatgtttt tagatggggt ggaaaaatcc
    18421 ttgtgtaggc aacataaatt caataaattt cttgataatt cgaaacctac taatagcgca
    18481 cctataaaag ctttttcatt acgttcagca tgacggtcac gtcgttcata ttttttacgc
    18541 ttgctgttcc ctgttattac agctaagcca agtgatatgg cgagaattgc ccaaacaata
    18601 gtacttaaat aacaattttc cccatactat caataaggaa agaaaaaacc ttttagtatt
    18661 aggtcgatag attataatcc atgcccatga aaatgcaata agagttatac ataagataca
    18721 agctgacagg atttctttat ttttaattaa ataacttaga gcgatgagga tgacaggtgt
    18781 caagaaaata atagttacat cccgatagct ataaaagaaa actgccctat tttgaatgtg
    18841 gagatgtgca cagaatccaa tatagctaag gataatagtt aatataataa aaaaagacca
    18901 ccaagggtga agagatagga attccatgtt ttcccttttt tgtaaaaagg aaaaaatctt
    18961 atctaatagt taattgccta accagccaga agaagtttaa aatctatccc aataattcaa
    19021 ccatctatac agaaagttca gcttatggaa acccacgaaa aaatccgcct gatgcgcgaa
    19081 ttgaataaat ggtcccagga ggatatggcg gaaaagctgg cgatgtcggc aggagggtat
    19141 gccaaaatcg aacggggcga aacgcagttg aatatcccgc gtttggagca gttggctcag
    19201 attttcaaaa tcgatatgtg ggacttgctc aaatcgggcg gtggtgggat ggtgtttcag
    19261 attaatgaag gtgatagtgg tggcgatatt gcgttgtatg cgtcgggtga tgtttcgatg
    19321 aaaatagaat ttttaaaaat ggagttgaaa cactgcaaag aaatgttgga acaaaaagac
    19381 aaagaaatcg agctgctccg caagctgacc gaaaccgttt aaacagatat gccgtctgaa
    19441 aaaagttttc agacggcata ttctttgaca ggtcttgtat aataccgttt gaacttacag
    19501 gtttttgatt atggcggcag gcaaacatac caaacacagc aaccgggtac gcattatcgg
    19561 cgggcaatgc cggggcagga aattgagttt cacatccgcc gacggactgc gtccgacacc
    19621 cgacagcgtg cgtgaaaagc tgtttaactg gctgggacag gatttgacgg gtaaaacggt
    19681 tttggatctc ttcggaggca gcggcgcact cggtatagaa gccgcttcgc gcaacgccaa
    19741 acgcgtgctg atttcggata acaaccgcca aaccgtgcag accttgcaga aaaacagtcg
    19801 cgaactgggt ttggggcagg tgcaaatcgt ctgttcagac ggcatcgcat atttgaagac
    19861 cgtatccgaa cagtttgatg ttgtctttct cgacccgccg tttgcatggc aggactggca
    19921 aatcctgttc gatgccttga agccgtgcct gaacccccgg gcattcgtct atctcgaggc
    19981 gggtacgctg ccggatattc ccgattggct gacggaatat agagagggga aatcggggca
    20041 gagtacattt gaattaaggg ttttccaagt ggctgaataa tatgcgcttt gataatcatt
    20101 tccgagttgt aaacattcgt ttgcaaccgt ccggttcaaa aaaaccttgt gctataatcc
    20161 gcgcccgccc ggttttgata atttagtgga aaaggaaaag aaatgtcgct ttttattacc
    20221 gacgagtgca tcaactgcga cgtatgcgaa cccgaatgcc ccaatgatgc catttcccaa
    20281 ggcgaggaaa tttacgaaat caaccccaac ctctgcacgc agtgcgtcgg acactacgat
    20341 gagccgcagt gccagcaggt ttgcccggtg gactgcatcc tgattgacga agaatatccc
    20401 gaaacccatg acgagttgat ggcgaaatac gaaaagatta tccagtttaa ataaattctt
    20461 tttaaaacat caaattatgt ctgttttgaa ataaaatcaa aaaaaaactt gacggaaaag
    20521 caagccgcta ataaactaac gttctctttt ggagggattc ccgagcggtc aaagggggca
    20581 gactgtaaat ctgttgcgaa agcttcgaag gttcgaatcc ttcttcctcc accaaaattc
    20641 ttacttgggg cagtagcgag taatgcgggt gtagctcaat ggtagagcag aagccttcca
    20701 agcttacggt gagggttcga ttcccttcac ccgctccaaa caattaggcc catgtagctc
    20761 aggggtagag cactcccttg gtaagggaga ggtcggcagt tcaaatctgc ccatgggcac
    20821 catccctcga ttattcattt ctttaaggct tagatatata ggatattg
//
LOCUS       DAXHTY010000029        20687 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXHTY010000029 DAXHTY010000000
VERSION     DAXHTY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.029"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(63..239)
                     /locus_tag="ACFNMK_000898"
     CDS             complement(63..239)
                     /locus_tag="ACFNMK_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364194.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVSAR"
     gene            complement(242..1600)
                     /gene="murF"
                     /locus_tag="ACFNMK_000899"
     CDS             complement(242..1600)
                     /gene="murF"
                     /locus_tag="ACFNMK_000899"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC6364195.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1725..3371)
                     /locus_tag="ACFNMK_000900"
     CDS             complement(1725..3371)
                     /locus_tag="ACFNMK_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6364196.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDAQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3394..4872)
                     /locus_tag="ACFNMK_000901"
     CDS             complement(3394..4872)
                     /locus_tag="ACFNMK_000901"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC6364197.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFRTPWGEGKCHTRLLGRFNAQNLAACVALLCANGYPLDKVLDVLEKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4897..6642)
                     /locus_tag="ACFNMK_000902"
     CDS             complement(4897..6642)
                     /locus_tag="ACFNMK_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC6364198.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6703..6966)
                     /gene="ftsL"
                     /locus_tag="ACFNMK_000903"
     CDS             complement(6703..6966)
                     /gene="ftsL"
                     /locus_tag="ACFNMK_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC6364199.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(6963..7949)
                     /gene="rsmH"
                     /locus_tag="ACFNMK_000904"
     CDS             complement(6963..7949)
                     /gene="rsmH"
                     /locus_tag="ACFNMK_000904"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC6364200.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTMRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7946..8401)
                     /gene="mraZ"
                     /locus_tag="ACFNMK_000905"
     CDS             complement(7946..8401)
                     /gene="mraZ"
                     /locus_tag="ACFNMK_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC6364201.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            8680..9897
                     /locus_tag="ACFNMK_000906"
     CDS             8680..9897
                     /locus_tag="ACFNMK_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6364202.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPPLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNINID
                     WTGFCFGDICHKL"
     gene            10029..10105
                     /locus_tag="ACFNMK_000907"
     tRNA            10029..10105
                     /locus_tag="ACFNMK_000907"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10063..10065,aa:Met,seq:cat)
     gene            10113..10188
                     /locus_tag="ACFNMK_000908"
     tRNA            10113..10188
                     /locus_tag="ACFNMK_000908"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10146..10148,aa:Ala,seq:ggc)
     gene            complement(10479..11300)
                     /locus_tag="ACFNMK_000909"
     CDS             complement(10479..11300)
                     /locus_tag="ACFNMK_000909"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC6364203.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEHLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(11333..11977)
                     /locus_tag="ACFNMK_000910"
     CDS             complement(11333..11977)
                     /locus_tag="ACFNMK_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6364204.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQKQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11980..12825)
                     /gene="ftsN"
                     /locus_tag="ACFNMK_000911"
     CDS             complement(11980..12825)
                     /gene="ftsN"
                     /locus_tag="ACFNMK_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6364205.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALYEPDAATEAEQSDAEKAADKADEVEEK
                     ADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEKKASKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(12931..14427)
                     /locus_tag="ACFNMK_000912"
     CDS             complement(12931..14427)
                     /locus_tag="ACFNMK_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC6364206.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPKMPSEHGGQPDLCDVKGQHTARLALEISAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSHRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14441..14761)
                     /locus_tag="ACFNMK_000913"
     CDS             complement(14441..14761)
                     /locus_tag="ACFNMK_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC6364207.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15244..16770
                     /gene="putP"
                     /locus_tag="ACFNMK_000914"
     CDS             15244..16770
                     /gene="putP"
                     /locus_tag="ACFNMK_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC6364208.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            complement(16860..17027)
                     /locus_tag="ACFNMK_000915"
     CDS             complement(16860..17027)
                     /locus_tag="ACFNMK_000915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364209.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            17019..20624
                     /gene="putA"
                     /locus_tag="ACFNMK_000916"
     CDS             17019..20624
                     /gene="putA"
                     /locus_tag="ACFNMK_000916"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC6364210.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHCRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDCLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDTKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRNDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     SVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDNVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETVGISHVAALSP
                     LNSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4835 a         5984 c         5055 g         4813 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagagacttt cccaatcacg caaatcgggt aactccccgg atttttgccg
      121 ccacccaagt ctaaacataa atcttcataa agatacgcct gcgtctgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcaacaat aaggctttaa aaaaacgtcg acttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gagcatacgc gccgacttcg gcgtgcatgg
      481 cggcggcttc gtcctcaccc agttcgccca tatcgcccat cacgaaaata cgcggcgcag
      541 gcatacgcgc caacacgtca atcgcggctt tcatgctgtc gggattcgca ttataagtat
      601 cgtcaatcag ggtcgcgccc ttgatgccgg ctttgacgtt cagacggcct ttgatattgc
      661 tgaagccttt caaaccttcc gccacatcgt tcaaactcaa acctgcagcc aaagacagcg
      721 cggcggcagc ggcggcgttg tggacattgt ggcggccggg aacaggcagc accacggcgg
      781 cgcgttcgtc gccgcacacc aaatcaaatt cgcacgacaa cggtttcagc acgatatttt
      841 ccgcgtggac atcgccgcta tcgatgccga aagtgcgcgt attcaaatta agcgttgccg
      901 ttttgaagac agccatattg gcatcttctt gaggaatcag tgccatgccg tctgaacata
      961 agccttgata aatctcgctt ttcgctttgg caatatcgcc cactccgtcg aaaccgcagc
     1021 cgacatgggc gcgcatggcg ttgttgacca atgcggcatc gggtttggcg atttgtgtca
     1081 aaaccgccag ttcgccaaaa tggttcatac ccatttcaat cacggcatag cggtgttttt
     1141 cgtttaattt caacaaagtc aacggcaatc cgatgtggtt gttgaagttg cctgccgtcg
     1201 ccaaaacggc attatcgccg aaacggcggc gcaataccgc agccaacatt tccttcaccg
     1261 tcgtcttgcc gcccgagccg gtaataccga acacaaacgg gttcacattc tcgcgccacg
     1321 ccttcgccaa catttgcaac gcggtaagcg tgtcatcgac tttcaacgcg ccatccattg
     1381 caacgcaatc ttcgcgcgaa accacaaccg ccgccgcacc agcagccaat acgtcttcaa
     1441 caaaatcatg cgcatcaaac cgcccgcccg ccaatgcgaa aaacacatcg cccgcgcgga
     1501 tgtcgcggct gtcggttacg atgcgcgaca cgggtttgct ttcagacggc atcggaagct
     1561 tgagggcttg gcagatgaaa tttaggtcca gtggtttcat atttactttc gttaatattc
     1621 gggcggcgga cacatcggta gcggctgatt tttttatcgc ctgttttgct gtggtaaaac
     1681 acagattatt ttcccattct cattcggcat tttttctgta cgtatcattt tttagacgta
     1741 tttttagtcg atttgccttt tcccgcatac cacggcgcgg ggtcgtcggg cagtccgtcg
     1801 ataaaggcaa ggttattgcc ttcgccctgc gcatcgggaa cattccccca aaaatcatag
     1861 ccgtcatcgg gcaactcgtc ggtttcgata cccgtccaac tgccgaatcc gcgtaaaaaa
     1921 ttaaacgcgc tgcgcctcac tttaatcatt tcgcgccgcg tgtcatcgga cgaaatttta
     1981 accagcggca cgccgtagct ttgacgcttc cacgcaccat gtgtcaacgt tcgctcgccg
     2041 cctttaccga catgcatcaa accgtggtcg gaaaagtaaa ccaaagacca gctttcttta
     2101 ttttcattca atatcttaac tgtatcttct aaaaatttat cggtttgcgc gatggtggaa
     2161 acatagcagg atattttttc agtttgatac tgaaaccgcc gcgcatcctt atccaaacgt
     2221 gtgcaaaaat cactgtgcga acccatcagg tgcatcacaa tcagccgagg cttcgtgcct
     2281 gcatttccta tcaaaacccg tttgaacgcc ggcaacaaaa ggcggtcgct caaccccgcg
     2341 cttttgccat aatcacccct ttgggtaaac cacggataat cgctgcgtag ggcataggtg
     2401 gaaatttcgt tggcaaaatg ccccaacatt ccttgattag acagccacgc cgtccgaaaa
     2461 cccgcctgct tcgccaagct gacgatgtta ttgttcggtt ctcccggcag ccccaaagtc
     2521 tgcggcagcg aaagattcgt cgcgtgggcg gtcgattggt aaccgtttat caacagccct
     2581 ttggtctgac tcaaaaaagg cgtatcgggc aatgggaaac cgtaaacatt catataatcc
     2641 gaacgcgcgc tctcaccgat aaccacaaca taatttttat acttgggcgc aacatgacgg
     2701 atatgccatg ttgacgcttt ttttgctgct tccaaaatgt gggcgcgctt ggcggcatat
     2761 tgtgccggcg cggaagccaa atcgtaatac aggtcgaaaa tattcaacaa caggccggca
     2821 tcaggttctc gcaaatcttt atcgctggcg attttatcca tcaccgcgca ggacaaaatc
     2881 aaagtcaata atatagtcag ccatattttg ctgcggcgtt tatagttttt tacgtcagca
     2941 aatacccccc ccgatacaca atatctccaa actgtcaagc caaaaatgaa aaataatgcc
     3001 tgcacaaaat aaagcgaccc gggaagattg ccgacaaatt cacgcgcctc ggcaggattg
     3061 ctttccaata tcgaaccgac tatctgataa gacggcgcac catacagcca gccgaccggc
     3121 aaatataggg cggttgtgcc gacgtaaatc agtaaaacaa ctgaagaaac gcgcgggaaa
     3181 ccgcgtgcca ataataataa aaatacaacc gaagccaaag cacccacggc agaataacgg
     3241 tagccgtatt catattccaa gtgataacct gatgcgatgg ccgcgccaaa acaaaaggct
     3301 aaaagcgcgc aaatgcccaa tgtatttttt aatgtctgat ccatattttt tattcgggcg
     3361 gattgtttca tacgatgccg tccgaatatc ccattattta cgagttaaca aagcctgtcc
     3421 gacgatttca agatcggaaa aacggtgctt cacgccttgt acatcctgat agttttcatg
     3481 ccctttgccg gcaatcagga tgatgtcgtt tgcggcggct tgttcaaccg cataacggat
     3541 ggcggcggca cggtcggctt cgacgcattc gggcgcggga acggcaggca ggatgtcgtt
     3601 gatgatgtcg tgcggatttt ccaagcgcgg gttgtcgctg gtgacgacga ctttatccgc
     3661 gccctgtacg gctgccgcgc ccatcagcgg gcgtttgccg cgatcgcggt taccgccgca
     3721 accgaatacg caccacaaag ccgcaccctg cggtttgatt tcctgcaagg tggcgagtgc
     3781 tttttccaat gcgtcgggcg tatgggcata atcgacaacg accaaaggtt tgccgctgtt
     3841 catgatgcag tccatgcgcc ccgaagcggg acggattttt tccagcacat ccaatacttt
     3901 atccagcgga tagccgttgg cgcaaagcaa agcgacacag gcggcgagat tttgcgcgtt
     3961 gaaccgtccg agcaggcgcg tgtggcattt cccttcgccc cacggggttc ggaatacggc
     4021 ttctatgccg tctgaagagg cggtaaagtc ggtaatgcgg atgtcggcgt gttcgctgaa
     4081 gccgtagcta taaacggcca aatcggggca gtcttttttc agacgacctg cgagttccgc
     4141 gccgtattcg tcatccacgt tgatgactgc gtgtttcaag ccgtgccagt aaaacaggcg
     4201 cgacttgatg gcaccgtagg cttccatcgt gccgtggtag tcgaggtggt cgcgggtgag
     4261 gttggtaaag attgcgctgc ggaatgacac gccgttgacg cgcgactggt caagcccgtg
     4321 gctggagact tccatcgcgg cgactgttgc gccttgttga cggaaacggt agagcagggt
     4381 ttggacatcg acgggggcgg gtgtggtatg cgtggtttct tccaatgcac cccaaaagcc
     4441 gttgccgacc gtgccgacaa tggcggtttt ttcgcccaac aaatcggcag cttgcgccag
     4501 ccattgcgtg atggaggttt tgccgttggt tccggttacg ccccagaatt tgagaccgtc
     4561 tgaaacgttg ccgtaaactt gcgccgccaa tatgccggca cggtgtttca aatctttgat
     4621 gccttgattg gggactttcc attcgggatt ccacgcaaat ttgccgtcgt cgtcccaaaa
     4681 aacaaaagcc gcgccgttgg caacggcggc ggggatataa ctgcgtccgt cggcatattc
     4741 gcccggacag gcaacgaaaa tatcgccttg tttgatttgg cggctgtctg aatgcaacaa
     4801 acgccctgcc gcgtttgcac acgacagggt cgggatgccg gtttcagcca aaggggttaa
     4861 cttgctgaac ataaaacaat ctcgttgata ctcggattaa gacggtgttt tgacggctgc
     4921 ggcggtcagg ggcttggtcg gggaaatgcc caagatgttc aggctgccgc ccataatttt
     4981 tttgaagggc ggccctgcca ctacgccgcc gtaataaccg tgggcagtcg gttcgtcaat
     5041 ggttaccgcc acaatcacac gggggttttt ggcgggggca aaaccgataa aggtagcgat
     5101 gtgtttgttg tcggcataac gcccgttgac gaacttgcgc gccgtgccgg ttttcgcgcc
     5161 gacatcgaaa ccgtccaccg cacccgccgt accggtgccg cccggctcgg tcacggaaac
     5221 catcagattg cgtacctcgc gcgcggtcga ttctttgaat atgcgtttgc cttgcggcgc
     5281 aaccgcctgt ttttcaaagc tgaccggcag caaaacgccg tcgtgcgtca gtgcggtata
     5341 ggcgcgcgcc aattgcagca ggctcaattg caggccgtaa ccgaaagaca tcgtcgcctg
     5401 ttcaatgggc cgccacctgc gccaatttct caacaaacct gcagtttcgc ccggaaagcc
     5461 cgagtgcata cgcacaccga tgcccaactc atgatagaag tcatacattt cttcggcgcc
     5521 gaaacgcgca gacagtttgc ttgtgccgac gttggacgat ttctgcatga tgccgcgcac
     5581 atccaaagag gggtaaacat gggtatcgcg cacgggagac ggtccgattt tataaggctg
     5641 cgtattcagc cgttcgttca aatcggtttt gcccgcatcc aatgccttcg caatcacaaa
     5701 cggtttgatt gccgaaccgg gttcgatcat atcggttacg gcacggttgc gccgctgttc
     5761 gctgtctgcc cggccgggcc tgttgggatc gtaggcgggc gtattggcca aggcgaggat
     5821 ttcccccgtg cgggcatcca aaaccaccac cgttccggct tttgcctgat ggtattcgac
     5881 cgccttgttc aactcttcat aggccaaggt ctgaatcctc tgatcgaggg aaaggatgat
     5941 gtctttgccg tttttcgggg ctttattgcg cggggagtcc aagctgtcca caatattgcc
     6001 ctgccggtcc cgcaaaacga cttccgcgcc gtcttcgcca tgcaggctgt cttcaagcga
     6061 aagttccaaa ccttcctgac ctttgccgtc aatatcggta aatccgatga cgtgtgcaaa
     6121 caggttgccc atcgggtaat ggcgttttaa ttctttttca aatacaaagt tttccaaacc
     6181 caaggctttg acctcttcgg caaccttggg atcgagctgc cgcttaatcc agataaacga
     6241 cttgcctttc tgttcgagct tgttcctcaa aacatcaacc ggcacatcga caagctcgga
     6301 caggcgttcc aattgtgcgg cagacggcat ttccttcatc tctttaggca cggcaaacag
     6361 ggactccgtc ggcgcactca acgccaaaac cgcaccgttc cggtccgaaa ccgtaccgcg
     6421 tgtagccggc aatgtttgag tccgcacaat ccggttgtcg ccctgttctt tcaaaaagtt
     6481 atacgttacc gtctgcagat acagtccgcg agcaatcaga ccggcaaaca agaccgctat
     6541 tgccatcagg acgaagctga tccgtccgtt actggtcatc ggctttttga cctgctcttc
     6601 tttgggcagc atccgaggct tatattcgct cttaatcaac atttttactt ctcgttatta
     6661 ttatcctgac gcaggaatcc gattccggca cacaggctgc ttctatcttt gatgctccac
     6721 cataaaggta ttgcccgaaa ccggcggatg gaggttttgt ttttctgccg ccgccctgat
     6781 cgcttcgtgg ttcgccaaac gcgcctgttg cagcctcatt tgtgcataat cctgctccaa
     6841 ggcgatttcc tgttttttcg ccttgtccaa agctgtgaag ttgagcctgt actggttttg
     6901 ctgcatcaca acggaaaaag cggaaacgca caccgcaagc agcagaagga aattcaattt
     6961 gttcattgcc attcagacgt ttttctctgt gattgttccg gtatcggacc ggcagtccgc
     7021 tccgccacac gcaaaaccgc acttctcgcc ctcggattgg cggcaatttc cgcctcaccc
     7081 ggctttaatg ccctgcccac gattttcagg ggcggctcgg gcaaatccgc ttccctgacc
     7141 gccgcccaac gcggcagggg cgcgtgttgc gaatattttt tgacaaactg cttcacaatg
     7201 cggtcttcca acgaatggaa agcaatgacc gccaaacgtc cgccctcttt cagacgacac
     7261 atgacctgcg gcaatactgc ccctacttct tcaagctcgc ggttaataaa gatgcggacc
     7321 gcctggaagg tgcgcgtcgc aggatcctgc ccccgctcgc gagtacggac gttttgtgcc
     7381 acgatctgcg ccagcttgcg ggttgtatcg attggacttt ccgcccgttg cgcgacaatg
     7441 gcgcgcgcaa tctggcggct aaaccgctct tcaccataat tcttgattac ctcgtgcaaa
     7501 tcctgttccg acgcaaccgc tatccactct gcggcagaca taccgcgcat cgtatccata
     7561 cgcatatcca aaggggcatc gaaacgaaag ctgaaaccgc ggctgccgtc atcgatttgc
     7621 ggagacgaaa tccccaaatc aaacagcgca ccgtccacct tgccgatacc caaaccgtcc
     7681 aatgccgtct gaaacgaagc aaaaccgcca tgcacgacac cgacccgttt gtccgaacgc
     7741 gccagctctt ctgccacagc aatcgcctgc gggtctttgt cgaaaacaat caaccgcccc
     7801 gcatcgccca aacgcgacaa aatcagccgg gaatgccctc ccctgccgaa cgtaccgtcc
     7861 acatagacac cgtcttcgcg cacggcaagc gcatccaccg cctcattcag caagaccgtg
     7921 atatgccggt aactttctgc tccactcaca attgcaaatc cgtctgaccc aactggaagg
     7981 caagttcgtc aggatcgtca tccaaagcct gaaccatctc agcctcccac tgctcgcgac
     8041 cccaaagctc caaacggttg gcacgaccga ccaaaacgac ttcacggtcg aaatccaccc
     8101 tcttcctcag tccggcagga agcagcaccc ggccggcgct gtcccattcc aaaatttccg
     8161 cgttatgcag caaaagattt tgaaaccgcc gcaaaacagg gttatccgcc acttttaagt
     8221 ttaaaagttg cgccgcaacc ttttcccact ccgcaacagg gtacatcaac agcttgtgtt
     8281 tcgactcgag cgttaccact acggcaggcg tatagaggcg cgacagaatg tcacggaatt
     8341 tggcaggaac agccaaccgc cccttactgt cgatgcttaa ttcgtgtgcg ccgccgaaca
     8401 tgacatgtcc caagccgaaa tcaaaatcac aagggtaaaa gagacacttt gccccacaat
     8461 tccccaccaa tcgacactat aagaaatttt aaacactcgg tcaaatcagg gcatgaaaac
     8521 tcattaacat atctgaaatt tttattcctt ttaaaaacaa taagataaaa aatgacgaca
     8581 acggcacggc gggtgcggta cagagtgacc gaaccaataa acaactatat atgattaatt
     8641 taataatata aacacaatat atagtattaa gataaagcca tgacagcacc cgtaccaacg
     8701 tgtaatatgt cgggaaatcc aataaattta cacaagctaa cacttatcat gcccctcccc
     8761 ctcccctctc ccgaagcacg gcaattctcg ctcaaactgc aaaccctcat tgccgaaaaa
     8821 atcggcaaac acggcaactg gattccattt tcacgtttta tggaattggt tttatacgct
     8881 ccgcaatacg gctactacac cggcggcagc cataaaatcg gcaataccgg ggattttatt
     8941 accgcaccgc ccctcacctc tctgtttgca cagacactgg cacgccaact tcaagaactt
     9001 ctatcccaaa cggcgggcaa tatctatgaa ttcggcgcgg gaaccggaca gcttgccgcc
     9061 gatttgttgg gcagcatttc ggacggcatc agccgttact atattattga aatatcgccg
     9121 gagctggcag cacgtcagaa aaacctgatt caagcacgcg caccggaagc atctcaaaaa
     9181 gttgtccact tgaccgcact tcccgaagcg tttgacggca tcatcatcgg caacgaagta
     9241 ctcgatgcca tgcctgtcga aatcgtccgt aaaaatgaag gcggctcatt cgagcatgtc
     9301 ggcgtttgcc tagataatga ccgttttacc tattcggcac gaccgctgca cgacttacag
     9361 ctatctgcct tggcttccct ctattttcct caaacagatt atccctacac cagcgaacta
     9421 catccgcaac aatatgcctt tatccgcacc cttgcctcaa ggcttgaacg cgggggtatg
     9481 atattcatcg actacggttt tgatgcggcg cagtattacc accctcaacg caatcaaggt
     9541 actctgatcg gacactaccg acatcacgtt atccacaatc cttttgactt catcgggttg
     9601 gccgacctga ccgcgcatgt caactttacc gacattgccc aagccggtac ggatgccgga
     9661 ttagatttga taggttacct tccccaatcc catttcttat tgaacctggg cattaccgag
     9721 ctattggcac agacggggaa aacggattcg gcagcctata tccgtgaagc cgccgctgtt
     9781 caaaaactaa tcgatcaaca tgaaatgggc gaactgttta aagtcatcgc attcggtaaa
     9841 aatatcaata ttgattggac aggattttgt tttggcgaca tctgccacaa actctaaccc
     9901 tcatgccgcc tgaatccgct tcagacgaca taaacttttt aacatttaaa aacagtcaac
     9961 taattcaaaa ttaaaaaata cggcttgtca aaaaaacaga aaaacatata atagtgtctt
    10021 cacgaaacgg cgaattagct cagtcggtta gagcagagga atcataatcc ttgtgtccgg
    10081 ggttcgaatc cctgattcgc caccaaattt tcgggggtat agctcagttg gtagagcgct
    10141 tgcatggcat gcaagaggtc agcggttcga tcccgcttac ctccaccaga taaaaaagca
    10201 cagaccgtaa aaaggtatgt gcttttttat tacctgattg ccgggaaata aagaacaaac
    10261 cactgccttc aaaacaggca atcgacttta aacctatcgc cccgcctgtc ctgattttat
    10321 agtgaattaa atttaaaccg gtacagcgtt ggctcgcctt gccgtactat ctgtactgtc
    10381 tgcggcttcg tcgccttgtc ctgatttttg ttaattcact atatcagccc gccagacaaa
    10441 cccgacccga ataatgtctt caggtcgggt ttatggtttc attcccaact tatccagccc
    10501 gacaaccaca atattatgat gacgatgccg aaaacaatgc ggtaataggc aaacgggata
    10561 tagtttttct tggaaacaaa cttcagcagt gctttgaccg ctactaaacc ggaaacaaaa
    10621 gcggcaataa agcctatcag aatcaaaccg acatcatgca gggtgaaaaa ccggtaatgt
    10681 ttcaggacat cataagccgt tgcggcaacc atcatcggaa cggccaagaa aaatgaaaac
    10741 tccgttgccg ttttccgctc gattccccaa agcatcccgc ccataatcgt actacccgaa
    10801 cgggacgtac ccggaaccag tgcaaacact tgggcaacgc cgatcatcaa cgcatcaatc
    10861 ggacgcaatg catcaacatc ggcaatttta ggctctgccc ggctttggcg tttctccacc
    10921 cacaaaataa aaaaaccgcc caaaaccagc atgaccgcca cactcaaagg attgaacaga
    10981 tgttctttaa tctgtttgcc gaacaacagc cccatcacgg cggcaggtat aaaagcaatg
    11041 gcaagattga ggacgaagcg gttggctttc cggtcttttc ccacgccatg caacacattg
    11101 ctgaaacgct gccggtattc aaacactacc gccaaaactg caccgagctg gatggcaatt
    11161 tcaaaaacct tgtgattgct gtgaaaatca atcagattgc cgaacacaat caaatgtccg
    11221 gtgctggaaa tcggtaaaaa ttcggtaaaa ccttctacca agcccatcat cagggctttc
    11281 aggacaatca gaaaatccat tgcttgcgct tctttcggat acgggagttc ggctatttct
    11341 gtacagcagg ggtctgacgc ttgcgttctt ccctgacttt ggcaaccaat tctttaatcg
    11401 tatgaacgcc gccgtcaaag ccattattga agataacgcg gtattttccg ccgacaataa
    11461 cggtcggcgt gctgtcaata ccgtattgtt ccgtcagttt ctgcattttt aatgcggcgg
    11521 cggcagcttc gggggaatca taggcgcgca tcagtttttt gccgtcaaag cctttttgag
    11581 acaaagccca ttttccggca acagccctgt tttccaagcg gattttttgt tcgtaaactg
    11641 ctttaaacac agcagggttt gcctgatatt tcaaacccga caaattgacg gcagccgcca
    11701 tcctggccag accgagcatt tcaggctgcc agaccacgtg ctccgtcctc aaataggcat
    11761 cagacggcaa tgccttgccc aatttcaata acaaaggatc gaaatgatgg caatgtacgc
    11821 agaaatagcc gaaaaattcc aaaacctcaa ttttgcccga ctgtttttga ggaatgggtt
    11881 tatccaacac aagatagtct tccccttccg tcagggcata tgcctgcgcg gacaacactg
    11941 ccgacagcag cagcggcaac agatgtttga gcttcataat tatttgcttt cgatagaacg
    12001 gatcaggctg gcgacttcat gttttttcaa ctcgtcctgc atttttttca ccgcatcggc
    12061 agacatattg ccgctttgca cccggtaaag cgttttatgt cccgcctgat aaccgaccac
    12121 cttggaagat atgcccaaga ttgccagttt ggcacgctgt ccttccgcgc tccggcggtc
    12181 ggcatacgcg cccatttgca gataatgcgt tgcttccgcc ttgtcgggcg ttttcatttt
    12241 ctgcacttct ttggcagcgg cactgcgcgc tttttcgatg ctgccgctgt tgaggatttg
    12301 ttccggggtc ggtttgggtg caactttttc cttctccgcc tttttctctt cttttgaagc
    12361 ttttttctct gtttctttag atggttttac cgcttgtttt ttaaccgttt cggcatcttt
    12421 cttctgcgct ttttccccga cggtttgttc acgctcttcc gtcagtgctt tcttgcgcac
    12481 tgcctgtccg tccgactttt cccgctccgg ctcgtccgcc ttttcctcaa cctcgtcggc
    12541 tttatcggca gctttttccg catccgactg ctctgcctct gtcgcagcat ccggttcgta
    12601 caaggcgttt tgatcggccg gttcaggttg gatgtcttcc ttaggctggt ttttcggttt
    12661 caggatttcc gtttctgcag gctgcttcga cgaagccggg attttgaacg cattttgacc
    12721 gctctggttc agataaaaca aaataccggc aataatgacc gtcgccagta tcaaaccgaa
    12781 gaaaaaaccg gacagacctt ttccggattg ggaaaatttg ttcataaaca taccttaatg
    12841 tgtttcagac ggcattaacg ccgtttgctt acgggcggat attctaacaa tatcgccata
    12901 tttgggcaaa acctgcttcc attcccgttc ctataaagcg cgacggaaac ctatggcttt
    12961 catgacgtgg cttctgccga cttcttcgtc gcccgccaaa tccgccaatg tacgcgccac
    13021 gcgcataatg cggtggaagc tgcgggcgga aagggagagt ttttccagca ggccgcccaa
    13081 tgcttcctgc gcttcttttt gaatgcgggc ttgggaatcg agttcgctga cgctcaaagc
    13141 ggcgttgact ttgccctgcc gtgcgtattg tttgtcgcgg gcggcgataa cgcgttccaa
    13201 aacggacgcg ctgctttcgc ccgcttcctg ctgcatcagt tcggcggcgg acaggctggg
    13261 gacttcgatg gtcaaatcga tgcggtcgag cagcgggccg gaaatcttgc tgcggtaacg
    13321 cgcgacgctt tcgggcgtgc agcggcaggg tttgacggga tgcccgagat aaccgcacgg
    13381 acacgggttc atggcggcga caagttggaa tttggcagga tagacggctt ggcgcgccgc
    13441 gcgggaaatg tggatttcgc cgttttccaa cggttcgcgc aaaacttcca aaactttgcg
    13501 gtcgaactcg ggcagctcgt ccaaaaacaa tacgccgtgg tgcgccaatg aaatctcgcc
    13561 cggacgcggg tcggaaccgc cgccgaccat agccgccgcg ctggcactgt gatgcggact
    13621 gcggaaagga cggtggctgt cgagttgttg ctggtggttg ggcaggagcg aacgcaatgc
    13681 ccaaacttca accaattcgt cttcggtcag cggcggcagg atgcctggca gccgttggga
    13741 gagcatagac ttgcccgttc ccggcggacc catcatcaag aggctgtgtc cgcctgcggc
    13801 agagatttcc aaagcaaggc gggcagtgtg ctgacctttc acatcacaca aatcaggttg
    13861 tccgccatgt tcagacggca ttttcggtac ggaacattcg gtttgcgcca aaggttcgat
    13921 accgttcaaa tgggcggcga cttcgcccaa agagcgcgcg ccgtaaacgg taatgccgcg
    13981 catcacggcg gcttgtcctg cgttttcttc aggcaaaaca aatgcacgtt ttgcctgcat
    14041 accctgccac gccatcgcca acgcgccgcg cacggggcgc aacagccccg acagtgccaa
    14101 ttcccccgca aactcgtatt ccgccagttt ttcgggcgca acctgccccg atgcggcaag
    14161 gatgccgatt gcaatcggca aatcgaaacg ccccgactct ttgggcaggt cggcgggggc
    14221 gaggttgacg gtgatttttt tggcggggaa ttcaaaaccg ctttgaataa tggcggcacg
    14281 gacgcggtcg cgactttctt tgacttccgt atcgggcagt ccgacgatgt tgaaatgtgg
    14341 caggccgttg gcaaggtggg cttccacttc gaccaacggc gcattcatac cgctcaaggc
    14401 gcggctgtaa accaaggcaa gcgacatatt tcagacggcc ttattcgccg gcttcggttt
    14461 gctgcctgat ttcggcgacg gcttcttcgg cagcggcttc agccgcttcc aatgctgccc
    14521 gttcgggatt ttgcgcggct tcgagttttg ccaaacgcgc ttccaaagcc gccagtttgg
    14581 tacgagtttt gattaaaacc tgctgctgga tgtcgaattc ttcgcgcgta accagatcca
    14641 tacggttgaa cgcgccgccc agcatcgcct taatattttt ttccacatct ttggcagggc
    14701 tgttggcgat ggtttcgctg attttcgaac cgacttcttc aaaaagctgc ttgccgaaca
    14761 taatctgtat ccttcctgaa catatcaaat tcaatcggct attgtataag gaaaaatgcc
    14821 gtctgaaaac gggcggcgga taatcggcaa aacataccgc gcctcctttg cggttgctaa
    14881 attacacctc aaaacacctg ccgcctgaaa acggatttca tattccgcgc aacgccagat
    14941 tacaagacac tacaaaacaa tatactgttt taaatgattt ttccgacgcg catcgtttca
    15001 aactcggctt tttaagccat taagtgcttt gcaaacaaca gaaattgggt tatgctgaaa
    15061 cggattattt acaatttcat atagttttat tacatatctt attgtgattg aagataattt
    15121 atccgaattc ccctttcggg tatccggatt ttccgttgta cttttattag aaaaacattt
    15181 caggcgcaag ttgcttgcaa tttcaaagcc tgtaattctg aagttttaga cgaggaatag
    15241 cacatgaatc ccatgtacat tacttttgca atctatctgg ttgcagtcct tctcatcggg
    15301 cttgccgcct atttttctac gcgcaatttc gacgattaca ttttgggcgg gcgcagcctg
    15361 ggcccgtttg ttaccgcgat gtcggcaggc gcgtccgata tgtccggctg gctgctgatg
    15421 ggtctgccgg gcgcgattta cctgagcggt ttgaatgagg cttggattgc catcggcctc
    15481 ttggtcggcg cgtatttcaa ctggctcttg gtggcgggcc gcctgcgcgt gcataccgaa
    15541 tacgccaaca atgcgctgac gctgccggat tatttcttcc accgcttcgg cgcgggcgga
    15601 cacttgatga aagtggtttc cgcactgatt atcctgttct tcttcacgat ttattgcgcc
    15661 tcgggcattg tggcgggcgc aaccctgttc caaagcctgt ttgaaggtat gacttacaat
    15721 caggcaatgt ggctgggcgc gggcgcgacc atcgcctata ccttcttggg cggtttcttg
    15781 gcggtcagct ggacggatac gctgcaggct tctttgatga ttttcgccct gattttaacg
    15841 ccggtgatgg tctatctggg cttgggcggc gcggaacaga tgtctgccgc gattcaaagc
    15901 gtcgccgcag gcacgggcaa agaatacggc agcctgtttg ccggtacgac cgtcatcggc
    15961 atcatttcca ccgccgcatg gggcttgggc tatttcggac agccgcacat tttggcgcgc
    16021 tttatggcgg ccgaaagcgc gaaatcgctg gtatccgcac gccgcatcgg catgacttgg
    16081 atggcgttgt gccttgcggg cgcggtggcg gtcggttatt tcggcattgc gtatttcggt
    16141 gcaaatcccg acaaagtttc ttctatgagc ggcaaccacg aacgcatctt catcgcgctt
    16201 tccaccctgc tgttcaaccc ttggatcgcc ggcatcattt tgagcgcgat tttggcggcg
    16261 gtgatgtcca ccctgtcctg ccagcttttg gtttgctcca gcgcgattac cgaagacttc
    16321 tacaaaggct ttttgcgtaa aaacgcgcaa cagtcggaac tggtatgggt cggccgcctg
    16381 atggtcttgg cgattgccgt gatttccatc ctgattgctt ccgatccgaa cagcaaagta
    16441 ttgggactgg tgtcttatgc gtgggcaggt ttcggtgcgg cgttcggccc gattgtgatt
    16501 ctgtccgtat tgtggaaacg catcactgcc tacggcgcgt tgtcgggtat ggttgcaggt
    16561 gccttaaccg tagtcgtatg ggcggaatgg gtaaaaaaac cggctcaagc ggcaggcgaa
    16621 agcggcttgt tgacgatgta cgaaatcgtc ccgggcttta ttgtttgctt aatcgtcgcc
    16681 gtattggttt ccctgttcaa caaagaacct tcgcgcgaaa cccaagaacg ctttgaaaaa
    16741 gccgacgcag attaccgcgc cgcccgataa acccctttca gacggcacgg catcccggtt
    16801 cgtgccgtct gaacccgttt ccgggtcaaa cacaaataaa ccttgcctgc atacaggcct
    16861 tatttgtgtt ttacccgttt cattttcaaa gaaacggaag cacctaccct gcccgcattt
    16921 gaagccttgt atttcatctg cggatttatt acgatattac cgtattcagg ccgcaccgat
    16981 gccgcctgcc ccccgaaaaa ctttggagaa tccaaaaaat gtttcatttt gcatttccgg
    17041 cacaaactgc cctgcgccaa gcgataaccg atgcctaccg ccgtaatgaa atcgaagccg
    17101 tacaggatat gttgcaacgt gcacagatga gcgacgaaga gcgcaacgcc gcctccgagc
    17161 ttgcccgccg tttggttacc caagtccgcg ccggccgcac caaagccggc ggcgtggatg
    17221 cgctgatgca cgaattctca ctctccagcg aagaaggcat cgcactgatg tgtctggcgg
    17281 aagccctgct gcgtatcccc gacaacgcca cgcgcgaccg cctgattgcc gacaagattt
    17341 cagacggcaa ctggaaaagc catttgaaca acagcccttc cctcttcgtc aatgctgccg
    17401 cctggggcct gctgattacc ggcaaactga ccgccacaaa cgacaaacaa atgagttccg
    17461 cactcagccg cctgatcagc aaaggcggcg caccgctcat ccgccaaggc gtgaattacg
    17521 ccatgcgcct gttgggcaaa cagttcgtca ccggacagac cattgaagaa gccctgcaaa
    17581 acggcaaaga acgcgaaaaa atgggctacc gcttctcctt cgatatgttg ggcgaagccg
    17641 cctacaccca agccgatgcc gaccgctact accgcgacta tgtcgaagcc atccacgcca
    17701 tcggcaaaga tgcggcagga caaggcgttt acgaaggcaa cggcatttcc gtcaaactct
    17761 ccgccatcca tccgcgctac tcgcgcgccc aacactgccg cgtgatgggc gaactgttgc
    17821 cgcgcctgaa agagctgttc cttttgggca aaaaatacga tatcggtatc aacatcgatg
    17881 ccgaagaagc caaccgcttg gagctgtctt tggatttgat ggaggctttg gtttcagacc
    17941 ctgacttggc tggctacaaa ggtatcggtt tcgttgtcca agcctaccaa aaacgttgtc
    18001 cgttcgttat cgactacctg attgacctag cccgccgcaa caaccaaaaa ctgatgatcc
    18061 gcctcgtcaa aggcgcgtat tgggacagcg aaatcaaatg ggcgcaagtg gacggcttga
    18121 acggctatcc gacctacacc cgcaaagtcc acaccgacat ctcctacctc gcctgcgcgc
    18181 gcaaactgct ttccgcgcaa gacgcggtat tcccgcaatt tgccacgcac aacgcctaca
    18241 ccttaggcgc aatctaccaa atgggcaaag gcaaagactt tgaacaccaa tgcctgcacg
    18301 gcatgggcga aaccctgtac gaccaagtcg tcggcccgca aaacttaggc cgccgcgtgc
    18361 gcgtgtacgc cccggtcggc acacacgaaa ccctgctcgc ctacttggtg cgccgcctgt
    18421 tggaaaacgg cgcgaactcg tctttcgtca accaaatcgt cgatgaaaac atcagcatcg
    18481 actgcctgat taagagcccg ttcgacacca tcgccgaaca aggcatccac ctgcacaacg
    18541 ccctgccgct gccgcgcgat ttgtacggca aatgccgtct gaactcgcaa ggcgtggact
    18601 tgagcaacga aaacgtattg cagcagcttc aagaacagat gaacaaagcc gccgcacaag
    18661 acttccacgc cgcatccatc gtcaacggca aagcccgcga tgtcggcgaa gcgcaaccga
    18721 ttaaaaatcc tgccgaccac gacgacatcg tcggcacagt cagctttgcc gatgccgcgc
    18781 ttgcccaaga agcggttggc gcagccgttg ccgcgttccc cgaatggagt gcgacacctg
    18841 ccgccgaacg cgccgcctgc ctgcgccgtt tcgccgacct gctcgaacag cacacccccg
    18901 cgctgatgat gcttgccgtg cgcgaagcgg gcaaaacgct gaacaacgcc attgccgaag
    18961 tgcgcgaagc cgtcgatttc tgccgctact acgcaaacga agccgaacac accctgcctc
    19021 aagacacaaa agccgtcggc gcgattgtcg ccatcagccc gtggaacttc ccgctcgcca
    19081 tctttaccgg cgaagtcgtg tccgcattgg cggcaggcaa caccgttatc gccaaacccg
    19141 ccgaacaaac cagcctgatt gccggttatg ccgtttccct catgcacgaa gccggcatcc
    19201 cgacttccgc cctgcaactc gtcctcggcg caggcgacgt gggtgcggca ttgaccaacg
    19261 atgcccgcat cggcggcgta attttcaccg gctcgaccga agtggcgcgc ctgatcaaca
    19321 aagcccttgc caaacgcaac gacaatcccg tcctgattgc cgaaaccggc ggacaaaacg
    19381 ccatgattgt cgattccacc gcacttgccg aacaagtctg cgccgacgta ttgaactccg
    19441 ccttcgacag cgcgggacaa cgctgctccg ccctgcgtat cttgtgcgtc caagaagacg
    19501 ttgccgaccg tatgctcgac atgatcaaag gcgcgatgga cgaactcgtc gtcggcaaac
    19561 cgattcaact caccaccgat gtcggctccg tcatcgatgc cgaagcgcag caaaacctgt
    19621 tgaaccacat caacaaaatg aaaggcgttg ccaagtctta ccacgaagtc aaaaccgctg
    19681 ccgatgtcga ttccgaaaaa tccacgttcg tccgccccat cctgtttgaa ttgaacaacc
    19741 tcaacgaact gcaacgcgaa gtcttcggtc ccgtcctgca cgtcgtccgc taccgcgccg
    19801 acgaactcga caacgtcatc gaccaaatca acagcaaagg ctacgccctg acccacggcg
    19861 tacacagccg catcgaaggc acggtacgcc acatccgcag ccgcatcgaa gccggcaacg
    19921 tttacgtcaa ccgcaacatc gtcggcgcag tcgtcggcgt acagcccttc ggcggacacg
    19981 gtctgtccgg cacaggcccc aaagcaggcg gttcattcta cctgcaaaaa ctgacccgcg
    20041 ccggcgaatg ggttgccccg accctgagcc aaatcggaca ggcggacgaa gccgcactca
    20101 aacgcctcga agcactggtt cacaaactgc cgttcaacgc cgaagagaaa aaagccgcag
    20161 cggccgcttt gggacacgcc cgcatccgca ccctgcgccg tgccgaaacc gtccttaccg
    20221 gaccgaccgg cgagcgcaac agcatctcat ggcacgcgcc caaacgcgtt tggatacacg
    20281 gcggcagcac ggttcaagcc tttgccgcac tgaccgaact tgccgcctcc ggcatacagg
    20341 cagtggtcga acccgacagc cccttggctt cctacactgc cgacttggaa ggtctgctgc
    20401 tggtcaacgg caaacccgaa accgtcggca tcagccacgt tgccgccctg tcgcctttga
    20461 acagcgcgcg taaacaggaa cttgccgccc acgacggcgc actcatccgc atcctccctt
    20521 cggaaaacgg actcgacatc ctgcaagtgt ttgaagaaat ctcttgcagc gtcaacacca
    20581 cagccgccgg cggcaacgcc agcctgatgg cggttgccga ctgattttac cgaaatcccc
    20641 gagcgcggcc cgtgaaccaa tgccgtctga aaacctttca gacggca
//
LOCUS       DAXHTY010000030        20497 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXHTY010000030 DAXHTY010000000
VERSION     DAXHTY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.030"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(209..284)
                     /locus_tag="ACFNMK_000917"
     tRNA            complement(209..284)
                     /locus_tag="ACFNMK_000917"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Asn,seq:gtt)
     gene            complement(377..736)
                     /locus_tag="ACFNMK_000918"
     CDS             complement(377..736)
                     /locus_tag="ACFNMK_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC6364211.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(759..1403)
                     /locus_tag="ACFNMK_000919"
     CDS             complement(759..1403)
                     /locus_tag="ACFNMK_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFC6364212.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1405..1941)
                     /locus_tag="ACFNMK_000920"
     CDS             complement(1405..1941)
                     /locus_tag="ACFNMK_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HFC6364213.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1953..3050)
                     /locus_tag="ACFNMK_000921"
     CDS             complement(1953..3050)
                     /locus_tag="ACFNMK_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6364214.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTSSQSQSRGLLQRLMKKLLGS"
     gene            complement(3053..4489)
                     /locus_tag="ACFNMK_000922"
     CDS             complement(3053..4489)
                     /locus_tag="ACFNMK_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6364215.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSRISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4709..5491)
                     /gene="bioC"
                     /locus_tag="ACFNMK_000923"
     CDS             complement(4709..5491)
                     /gene="bioC"
                     /locus_tag="ACFNMK_000923"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC6364216.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMTHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCNVQPQLTEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFITHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFTVILDFDTPSDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            complement(5479..6126)
                     /locus_tag="ACFNMK_000924"
     CDS             complement(5479..6126)
                     /locus_tag="ACFNMK_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC6364217.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGMPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCT
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFGEIHQELIALFAMIGQDRR
                     TDLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDVGHYLFSRFTHWSALWNH"
     gene            complement(6136..7278)
                     /gene="bioF"
                     /locus_tag="ACFNMK_000925"
     CDS             complement(6136..7278)
                     /gene="bioF"
                     /locus_tag="ACFNMK_000925"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC6364218.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANIGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEAALAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAVCL"
     gene            7501..8577
                     /locus_tag="ACFNMK_000926"
     CDS             7501..8577
                     /locus_tag="ACFNMK_000926"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFC6364219.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKIIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYRQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8784..10250)
                     /locus_tag="ACFNMK_000927"
     CDS             complement(8784..10250)
                     /locus_tag="ACFNMK_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC6364220.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10448..11371)
                     /gene="speB"
                     /locus_tag="ACFNMK_000928"
     CDS             complement(10448..11371)
                     /gene="speB"
                     /locus_tag="ACFNMK_000928"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC6364221.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11470..13362)
                     /gene="speA"
                     /locus_tag="ACFNMK_000929"
     CDS             complement(11470..13362)
                     /gene="speA"
                     /locus_tag="ACFNMK_000929"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC6364222.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDFGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13529..13813)
                     /locus_tag="ACFNMK_000930"
     CDS             complement(13529..13813)
                     /locus_tag="ACFNMK_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6364223.1"
                     /translation="MKKLLLAAVVSLSAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13964..15772)
                     /gene="aspS"
                     /locus_tag="ACFNMK_000931"
     CDS             complement(13964..15772)
                     /gene="aspS"
                     /locus_tag="ACFNMK_000931"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC6364224.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGGFPRMPYAEA
                     MFYYGSDKPDMRIGLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVSNLSNGEDSGLQSPIVKFLSENALKEIIERTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(15831..16532)
                     /locus_tag="ACFNMK_000932"
     CDS             complement(15831..16532)
                     /locus_tag="ACFNMK_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC6364225.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16675..17823)
                     /locus_tag="ACFNMK_000933"
     CDS             complement(16675..17823)
                     /locus_tag="ACFNMK_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC6364226.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17984..18247)
                     /gene="rpsT"
                     /locus_tag="ACFNMK_000934"
     CDS             complement(17984..18247)
                     /gene="rpsT"
                     /locus_tag="ACFNMK_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC6364227.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18550..19692
                     /locus_tag="ACFNMK_000935"
     CDS             18550..19692
                     /locus_tag="ACFNMK_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC6364228.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5275 a         5303 c         4748 g         5171 t
ORIGIN      
        1 gcgagccaat gccgtactgg tttttgttaa tccactataa aatcgagttt atcctagttg
       61 tccaggacag cccctatttt ttcataacca tcacgaaagg aattttgcaa agattttatt
      121 ccatctcaaa acaatcatct caaaaatgcg tttctgaccg ccggtaaaac aaaaccctct
      181 aagaaaatac ttagagggtt ttgaaatttg gctccccgac ctgggctcga accagggacc
      241 tgcggattaa cagtccgtcg ctctaccgac tgagctatcg gggaataagc acgaataata
      301 aaacagaaat accgaacgtg tcaatataaa ttgacaatcg attgcacttt atctgcaagc
      361 ccgcaatctt tatatgttat atggcttccg caatatgcct tgccgcacgt ttggcatcca
      421 ataggattaa accaagtctg ccggtttctt ttgccaccag caccaacacg gcatctttac
      481 ccgcctgact taaaaggata tagcctgatt ttcctttaat catcacttgt tccaattccc
      541 cgcaggcgag ttcctgcacc gagcgactcc ccaaagcaag caaagtggca gaaatcgccc
      601 ctaccctgtc cgaattcaaa tgtgaaggaa gcattgtcgc catcggcaat ccgtcggttg
      661 agataacggc agacgcgata atatccgtag atgtattgtt taaatcttca aggattgaaa
      721 tcaataattg ctgcataacc cctcctctct cccaagtttt acacgcggtt gctgtaacgg
      781 cggtataaaa tccttaccaa agtaacaaat gcctctttgc ccaaatcggg aatgccgccg
      841 ataaccaaaa taaatttggt tgaaccgata tacaatggga aaaatgtcaa ttcgctctga
      901 ccggaaggat cgcaaacgcc ccaagcgtta ttgttgatat acaggttgtt cttaatcagc
      961 agccggtatt tcttttccat ctgtgcgact tctgccgcca acaaccccaa ctcttccgcc
     1021 gcctcatgat ggaaattggc gttggcaaga tacagaccgt tccgatcgac caataacgcc
     1081 ttaccgctgc cggacaattg ttccatcagc aacggcaatt gctcgtccga caaattgatg
     1141 ccgtctgaat gaccgttttc atcgccatag aggaattcga gtttttgcaa acggtacaac
     1201 aggttcaaag cggtatcgat gtcggcggtg tccgcccaag taagcagctt ctcactgctg
     1261 accatttcgt ccgcatccgc tttcaacagg ctgtgcaaca aagttttacc ggcactgggg
     1321 gcatcgctgg atacggcata aaatgcaccg gcaggagtca ggcggggata taaatttgct
     1381 tgtagtgaaa gtgttgattc catattaaac ctccagtccc ggatcaatag aaaataacat
     1441 tgcgctaacc aattgtttta cgtcatcttc cttacgggca tcaatttcaa aaaccggaac
     1501 attaagatta tgttttgcaa gatatttgtg atacacgtcg ataccgggct gagagcgtat
     1561 atccatcttg gtaataccga caacgacggg tgccttctcc agcagccctc gaaacgaatg
     1621 taaaaagaat tccaaatctt tcaacggatt ggttcgggca ttatctaaaa gcaagaccaa
     1681 acccatactg ccttggctta agatttccca cataaagttg aaccgttcct gaccgggcgt
     1741 accatataaa tggactttgg tatcctcatc caagctgatg gccccgtagt ccatcgccac
     1801 tgtcgtattc cttttcctat ccaaagtcat atcggatgcg gaagcatcgg tctgaacgag
     1861 tgcttcgtcc gaaatagccg caatggcagt ggttttccct acgccgacag gtcctgtgaa
     1921 aataatttta ttttctctca tctctccgcc tcttagctgc ccaacagttt tttcatcagc
     1981 ctttgcagaa gaccgcgcga ctgggattgg gatgatgttg tgattttttc cgcttttttc
     2041 atcatttcac tatcagaggc agaatcggct ccgatattta ttttatccgc catatcggag
     2101 tatgcctgtt gtgaaaccgt ttttaaatct accgacaaaa acccggttgt ataggttgcc
     2161 gcaagataat tcagaatatc attgaggttt aaaggcatca ctttatacaa cacgttaagg
     2221 ttgacggatg ccttggtcag aaatgccgac aagcgtatcg accccggcac atttgccaac
     2281 cgggtcaggt ttggccaaga tttcaacgta aacggagtat cgggagaaat cggataaatc
     2341 aacctgccct gcgctgtcca aatggaaaac tgccacatac aggacataat gcctacttta
     2401 gccttttcgc gccattgcgg gttatcggga acagtcttgc agctgacctg caaattttcg
     2461 tctttgcaca attcttcgag tttttgcaca ctttctgtca gcaaaacccg ttgtatcgag
     2521 gggaaaacaa taaggaccgg cttatttcca tgcaagatag cgatgtcctg cctgttcttt
     2581 tccgcaaacc gcaacgcccc caataatcct ttattcgggt taaactggcg tatcgtaacc
     2641 gtacgctgca cattcccgtt atttttcgcc gaccggtctg cgggtgcaat aaacgatttc
     2701 ccataaacat tctcgccctg caacaatttg cggagcatag gaaacaatgt ttcaaaccga
     2761 atcggtttgg gcaggtaggg aacttcagaa tcgggaactt tctccgaaca gacggcgacg
     2821 ggtatatcct tataacgctc ggcaagctct ttccaaagtt caaaaccgcc ctcggcatcg
     2881 gtatccgcca aaaccaaatc gggcacggca ctgccgtctg aaggggatac tgtttcataa
     2941 cgggtggtat tgtgcatttt gaatgccatt ttgaaaacgg attcctgctg cgccgtcatc
     3001 cccgccaaca ttacgcgtac tgttttaatt ttcggcagtt gaacttccat ttttattttc
     3061 cgtaccgttt tattttttta atattttgat tcatacgctg cagcagccgg ctcatcagca
     3121 taacgacctc ttcaggaagc ctgtccgcac gttccctcaa tacccttaaa aactgcccca
     3181 acctatccca atcttcagta cgttcataaa tatcgatcaa cgtaatataa agctgggact
     3241 cgtcgggata tttcaatacc gcctgctcca acacatccat tgccgcttca atctgaccat
     3301 acatcagcaa cgactctact tccttaaccg catcgtctgc cggagacgaa ccggtgttga
     3361 tcaacgaaga atcttgaagc accaaatccc gatgttgcga tttgaatttc tgtatatttt
     3421 tcggcagata cccgtgcccc ataccgatgt ctttgatttg ccggtcgttc ggcccttttt
     3481 ccaaatcatc gaaaacttca tggtaaccca ggctgtaccc ccaccccagc atccgctctt
     3541 taacctgcct gccgtagttg cccaacgttt ggtaaagttt ccacaaatgt ccggcaaaac
     3601 ggtctatgtc cgcgtattgg taatcgagtt tcaacgcatc gataatcagg tttgcaggtt
     3661 tttcggaagt ttggatggca cggtcgtatt gtttcgatgc cgtttcatag ctgactttgt
     3721 ctttaaggat tttcgcacct tgatcggcac ggaccaaacc ggcaatcgca ccgatttcct
     3781 cttgactgat tctggacacg tcttttttgc cccgcacaat cgggatgcgc ttgatttctt
     3841 cggcttcata agccttaccg ccggcatccg gcgggggcga tgcggaagct gccgtcgcag
     3901 aaccgccttc cgcacgtttt tcaatctcct gagtacccca tcccaaacct tcttccgcca
     3961 agacgcggat acgcaaatgg tttgaatcgc gctgtaatgc ctgttcgata tattttgcca
     4021 aaagttcgga aggaatcagt ttgccgtatt tttgcagatt gtctgccaaa acatcgacat
     4081 cccccacttc gagattgata tcgagcagct cgcggataag gttttcaggt ttcgcttcac
     4141 catccggaat gccgtccaaa taggcagcca aagattcggc agccttgccc tgataaccga
     4201 attgcttata aacctgatac tccgtaagcg gatcgacttc ttgcgcggat acggcggcgg
     4261 atgcggtctc ggcactttcg ttccaagacc agtcgggttg cgtgccgtct gaaaccgatt
     4321 gtccaatctg atcgacccag tccgaatcat tgcttccggg tgtctgcccc tgcgtattgc
     4381 cgacaccttg ggatttgcgc ttcggtgttt tcttaccctg cttcgcacgc ataaccaaaa
     4441 gcatcagcaa taccgccagt gccaaaccga taattaatga gttttccaaa ggatactccc
     4501 gataccgtaa cgagcggata gctgaccgcc tgcaacggcc attgtccgct tttattatta
     4561 acgatttact gaaatagact tgtaagtttt aaatcatacc ataatttaac gtttaacaat
     4621 atgccttctg cacaagcctc gccatattac cttttaccca catcagtatc aatacccgat
     4681 ataaaaataa ctttgcccat aagctgcctt attgcctgcc cgccgcagag tagcgcgcga
     4741 taaaaaataa cggatgataa gtcaggcgca ctttgcccga cggcataccg aacgccgaca
     4801 agtaatcgcc aataaatcca ttgagatttt ttcttgtcca atttttttgg ttcgtggctg
     4861 ttacgcctgt atatttaagg tgtttgagta catctgacgg cgtatcaaag tctagtatta
     4921 ccgtaaaatc ctcacaccat aaaagctcaa aatctttggc taaccaagcc tgccattggg
     4981 ataaagtcgg gtaatttaag cctatatttg taatttggcg gacttctttt aaattgtctt
     5041 tgccaaaggt tgcaaccgcc aataatccgt ttgttttcaa gcctgttttg caatgggtga
     5101 taaaagcgtc gggttgatga aaccattgca cggcagatgc gcttgcgatt aagtcaaatt
     5161 gtcgttgaaa aggaaagttt tccgcatcgc cgcaataaaa atcaaaggat tgcggcagtt
     5221 tttcagtcag ttggggctgc acattgcaca aatcattaaa taaccaataa ttcgctgaaa
     5281 tctgtttttg cagcaaggca ctcaacattc ctgagccgca gcccaattcc aacacgtttt
     5341 ccaatggcat atccggcaaa taatcttgca aatgcgtcat taaattaatc gtcatttttt
     5401 gttggattaa ggcgtgccgg tcataatcgt ttaatgcttt ttggaaagcc tgccgaatgc
     5461 gcgatttatt tatggcagtc agtgattcca tagtgccgac caatgggtga atcttgaaaa
     5521 caggtaatgt ccgacgtcaa tttcccgaac ggtgcaacgc ggtgtccaat atcggtgctg
     5581 attggcaggc ataaaaattt tatcgcccga tccgaccaag gcatttgtcc agcggataag
     5641 atctgtacgt ctatcttgcc cgatcatcgc aaaaagtgcg ataagttctt gatgaatttc
     5701 gccaaacggg cgtgcgggaa attgttggta atcttcaaaa gatgctttat cgccacacat
     5761 tctgcgttca aattttaaac gggtgttttc cgtgaggttc tccaatgtgc ctttaaaaac
     5821 ggtgcaaggg ataccgaaat tatcatcgca aggcaaacct gtgccattca ctgccgttgc
     5881 ggattttaat cttattcctt gcaatgccct ctctgccgcc caaacgccca ttgaccacgc
     5941 caccaaacgg atgtgccgat aggcggaaaa atcaaaatcc aaatttaaat cttgataatc
     6001 atagcaaatc aataaatcgt gattttccgg caaaatcaaa tgatttacag catcgggcgg
     6061 cattccccaa cctgcaaaat acaggattaa atgtccgcct tgatgattgt aaaattttgt
     6121 tttcatatca tatccttaca ggcacaccgc aaactgccgc acttcatccg ttgtcatatc
     6181 tgccgttaaa gacaggcgga ttctggatgt gtttttgggt actgtcggcg gtctgatggg
     6241 caggcaataa taaccctgcc tttgcagata ttccgctttg gcaagggcgg cttcattccc
     6301 gcctaaaata taggggacga tacaggtttg gctcggcatt atttgcgtcc gatgcgccac
     6361 ttcccgccgt aaaaatgcac ttaactgctc aagatggctt ctttcttttg agaattgcgg
     6421 caatcgttca aaaataaaat aagtccaagc cacattaaac ggcggcaatg cggttgaaaa
     6481 aatcaatggg cgcatttgat taatcaaaca ttctttcaat acttggttgc agacggcata
     6541 cgcccccacc gaggctaagg ctttaccgaa agtgccgacc agtaaatcaa tctcggcaat
     6601 caaattatcc cgttcggcaa tccccaatcc gttttgccca taaacaccga ttgcgtgggc
     6661 ttcatccaca taaagataag tattgggaaa ctgttttttt aattggacaa gctgtttcaa
     6721 atccgccaca tcgccgtcca tactgaaaac agattcggta acgataaaag tgcggtcaaa
     6781 ttttccgacg tttttttcaa gcagattttt caaatgttca taatcattat gacgataacg
     6841 gaaaaacgca caccggctca aacggatgcc gtcaatcata ctggcgtgaa caaatttatc
     6901 tgccaaaatc aaacttttcg tcgtcgtcaa agcaggcaaa atgccgatat tggcgtgata
     6961 gccgctattg aacaataacg cgctttcccg ttggaaacgt tgtgcgacaa gctcttccaa
     7021 atcggtataa ataggaaagt tgcccgttaa taaacgcgat gaagaactgg taaaagaggg
     7081 aaaattaccg ccgtattgct gcaaaaaaga ccggcgcaag ttttcatctg atgccaaacc
     7141 caaataatca ttagacgaca tattcagcat tttgcagttt tcccgcgtaa tataccgccc
     7201 ttgatgaatc aaatccggaa tcgaacgata ttggttttgc gcgccgagtt gttcaagctg
     7261 ttgtttaaaa accttcatta tgatgtaaat attcctgagt taaagcctga accaacccag
     7321 catcaaacaa tcaagtaatg cccttttaat aaaaaccgtt ttgccgccga cctttgccac
     7381 acccctgcct tcataatcca aggcggaaat ttccgcgaca tttgtttccg ttgccattac
     7441 ccaatccgtc cgcactgtac cgaagcgcat attttcgcac aaattggtac ggtttcaggt
     7501 atgatttgcc gaaaacctat cggacacaca ccatattttg atatgaataa aacaatctgc
     7561 cgcactgctg cactattgat aagcggtttt tcatacgcaa acaccgtcat tcccgatgtc
     7621 tcccccgtcg cacaaggtca acatgtcttc attaacatcc ctcagcaacg cctgttcctc
     7681 tacaccgacg gcaaactgac caaggtttat cccgttgcag taggtcgggc gatgacgcaa
     7741 accaatctag gcgaacataa aatcggtgcg aaagcctata accctgtttg gtacatcccc
     7801 aaatccatac agaaagaacg cggcgacggc gtaaaaatca tagccgccgg cccggacaac
     7861 cctttggggc cggtttttgt ccgcttaggc gaccccaaac tcggtttggg catacacggg
     7921 accaatgcgc cggccagcgt ccccggggtt cggagtcacg gctgcgtccg catgaaatcg
     7981 cccgacgcgc tcgagtttgc caaaaccatc gccagcggct cgcccgcctc cgtcatctat
     8041 caaatggcgg gtctcaatga agatgcggat cgcaacctgt ggcttgccgc cttccgcgac
     8101 ccttacggta agaacaacct tgacatcgcc tctctgaaaa aaagtattgc gcaatgggca
     8161 aaaacacagg gtaaaaccat cgcgcccgag aaagtcgatg ctgtactcaa agaccgcacc
     8221 ggatcggccg tctgcctgac ctgcggcaaa aacggcaaga tgaagatgcc gctcaaatcg
     8281 ctggcgtgga tacagggttc ttcctcatac cgccaagctg aagtcattga acaaactgag
     8341 gaaaccaact ctgccgaaat atccgagaca cgcacgcccg aagtgcctga tgtacacacg
     8401 cccgaggcac aaccgcattt aaatacccaa tccgacggca cgccaactgc ctataccgaa
     8461 ccggctgccg attcatcgcc gcaagtagaa acacctgatc aggcagcttc cgagccggtt
     8521 gatgtattat tttcaataga tgtgatacgg cagggaaatt tgcgtttagg taattgaata
     8581 accttctgat tatttaatgc ttatgtttat cagaagttga taggcggttt ggtttttgtt
     8641 tggcttttcc tacaccgccg cctgccgctt tctgcaacat tcaagcgcac aaatatgcca
     8701 tctgaaggct ttagacggca tatttcacga taatagtcgc ccaatcaaat aaacacagat
     8761 tggaaattta ttttattttt cccttaccaa taccccagaa ctttccacca aatgctgccg
     8821 ataacgaaaa aaatcagaaa attgactacg ctcatgataa aacccgcctt ccaccattct
     8881 cccattgtgg tgtagcccga accgaaaatc acaggcgaag taccggtcgc ataatgagtg
     8941 agggtcatca taatgttgga tgcggccgcc atcatcagcg cggtcggcat cgccggtgca
     9001 ttcagtgaaa cggcagcagc gagaaatgcg ccgaacatag cggtaatatg tgcagtagta
     9061 ctggcaaaca tataatgcgc atacatataa gcaagcacga ggattacgcc cgcagccgtg
     9121 ccgctaacgc ccaaaccgcc gacactttcc gccaacactc cggagaacca tttaatcagt
     9181 ccgagtttat ttaaaaatgc ggccatcata atcaatgcgc caaaccaaat aatcgtatcc
     9241 cacgcacttt tttctttcaa aacatcgtcc caagtcaata caccggaaag caaaagcagg
     9301 cttaatccga taaatgcggt ggcggtggcg ttgatactaa aagcgtgatt gccggtaata
     9361 agggcgggaa catctgccca caacagcagc aagataccga aaatgaccgc cataatgatt
     9421 tcgtctgccg acattttacc catctccctc agacggtctt tggcaaattg aacggcattg
     9481 ggcgtttctt taatttcagg cggatacaaa aaatataaaa tcaaaggcat aacgaaaaag
     9541 gcgataacgc cgggaacagc cattgcccac gcccacgccc cccaagaaag acggaaacta
     9601 ctgcctaaat tttcggcaat caagttgacg attaaagggt tgggggcagt tgcagtaata
     9661 aacatagccg acgaaatggg attggaatga tagttgacca aagccaaata tttacccatc
     9721 ttgccttctg tgccttttgc gggattggag ccgtaactgc cggcaatcga ctgcataatc
     9781 ggatgtataa tgccgccgcc acgcgcggta ttggaagggg taacgggagc cagcagcagt
     9841 tcggaaagag cgagactgta accgatgccc agcgtttttc ttccaaaaac ggcgataaac
     9901 aaatatccga tacgcatccc cagccctgtt ttgagcaaac cgcgcgaaat cataactgcg
     9961 atggcaatca gccaaatcaa cggattggcg aacgcactca acgcatcgct catcgccgcg
    10021 cccggtttgt cggcggttac gccggttact gcgaccaacc cgacggcaat aatcgacagc
    10081 gcgcccaacg gcatggcctt gccgataatg gcggcaatca cgccgacaaa catggccagc
    10141 agcgtccaag cctgaggctt gaccccgtcg ggtacgggca gtgccaaaac cagggcgcac
    10201 aatactgcgg caatggcgag gggtatcggt ttgaaaccca atttcatcat attgacctcc
    10261 gtaaaaaaga ccgtcccgaa aaatcggaaa aataatattt aactaattgt tttataagat
    10321 atatttagat atttcaccgt ctttccgata tgcggctccg ggcaactttt gtttcagtat
    10381 ttgaattttc attagactga atacgccgtt tgaacggcac ggcgaaaacc cgggggatgc
    10441 cggacgttca gtcctttttc gcaccttgaa ggtaaagcat ttccaaggca attgtggcgc
    10501 cggccaaagc ggtaatgtcg gattggtcgt aagagggggc aacttctaca acatccatac
    10561 cgacgatgtc gagatccgtc agcccacgta ggatttttaa tgccctgtcg ctgctcaagc
    10621 cgccgcatac gggcgtaccg gtcccggggg cgaacgacgg gtcgaggcag tctatgtcga
    10681 aagtcaggta aacgggcata ttgccgacgg tttctttgat tttacggacg gtctcttcaa
    10741 cactgtcttc attgacttgg ggggcggtca acacagtaaa aggcaatttt ttactgtgtt
    10801 cggtgcgtat gccgatttgt acggaacggg acgggtcgat gaggccttcc ttgggggcgg
    10861 tatagaacat cgtaccgtgg tcgtattcgc tgccgttgtc gtaggtgtcg gtgtgcgcgt
    10921 caaaatgaat cagtgcgagt ttgccgaaat agcgggcgtg ggcgcgcaac aacggtaggg
    10981 taatgaaatg gtcgccgccc aaactcaaac agcgtttgcc ggaagaaagt aatttgccgg
    11041 cgtgcgcttc cattttttcg acaaaatccc tgctgtcgcc aaaagaaaaa accaagtcgc
    11101 cgcaatcaat aatgttcagg cgttcgcgca catcaaatgt ccatggaaac ctgcggtgct
    11161 cccaagcgag gttgacggag gcgcgccgga tggcttcagg accgaaacgc gcgccggaac
    11221 gccctgaaac cgccatatca taaggcacgc cggtaataac ccaatcggca tgactttcat
    11281 acggcataaa attaagcggc aggcgcaaaa acccgaaatt attggaaacg agggagttgt
    11341 cggtttgtcc tgccagtgtg ctgtattgca tcgtaatgat tccttgtaat tggtttcaat
    11401 cggtcgtgat gattggtgtt tgagtaagaa aatcgggctt cagacgacat atccgatgcc
    11461 ttgatgcgtc tattcgtctt ccaaataggt ataaccatta agccccgctt cgagttcttt
    11521 taagaaagac atagcctgcg aggcaggaag gtctgaatgt tcgatttgtt cgcgatagcg
    11581 tttcatcagc tctttcggat cttgataaac gtattcgagc atatcggcaa cggtgtttcc
    11641 ttcatcgtaa tcgatgacgg taaattgtcc gtcttcccct acaacaacat cggcagtggc
    11701 agtgtcgccg aaaagattgt gcatattgcc gagtatttcc tgatatgctc ccaccataaa
    11761 aaagcctaaa agcggcggct cttcttcggg ataatcaggc ataggcatcg taccggcgat
    11821 gccgtcgccg tcgatgtagt ggtcaatcgt accgtctgaa tcgcaggtaa tgtccaacaa
    11881 cacggcgcgg cgcgcaatcg gttcattcaa accggtaatg ggacaaacag ggaaaagttg
    11941 atctatgccc caagcatcgg gcaaagattg gaagagtgag aaattgacat acagcttatc
    12001 ggcaaaacgt tcttgcaatt cgtcaataat ggttcggtga gaccggtgtt tttcattaaa
    12061 caattcgccg acttcatgac agatatttaa atacagttgc tccgcccacg cacgttgcgc
    12121 caaactcaac agcccgacat tatactgatt atgcacatcg gcaagatcaa actgcccttc
    12181 gtgtatccag ctgcgtaagg aacgtttttc ccgcgaggcg gaaatatccg tccaagtttc
    12241 ccacatactg tgcaacacac gcggtgcttc gggcgatggc gcatccagcc ggcgcggttt
    12301 gtaacgttca acgcctataa cattggcaac caaaacggcg tgatgtgcgg taatgccgcg
    12361 cccgctctcg gtaatgattg tcggatgcgg cagcccgtgt tcgagacaag cctgactgat
    12421 gccccatacg actgtggcgg catattcgtt gaggctgtaa ttaacggaac aatccgattg
    12481 tgtgcggttt ccttcgtaat ccacgccaag cccgccgcct acatcaaaac agcggatatt
    12541 tacccccagt ttgtgcaact caacataaaa ccgagccgat tcgtgtacac ctgtggcaac
    12601 atcacggatg ttcccaagct gcgagcccaa atggaaatgc aaaagctgca ggcaatccag
    12661 cctgtttttt tgtttcaaaa tatcgaccag ttgcaaaact tgggaagccg acaagccgaa
    12721 ttttgatttt tccccacccg aagactgcca ttttcccgaa ccttgggaag ccagtctggc
    12781 gcgcacaccc aaacggggct tgatgccgag tttttccgcc tcttccaata ccatttgtat
    12841 ttcggacagc ttctcaatca ccaaataaac ctgatgcccc agtttttcgc ccatcaaggc
    12901 gaaacggata tattcacggt ctttatagcc gttgcagacg attaatgttt gccgggtgcc
    12961 ggcgtgtgcc aaaaccgcca tcagttcggc tttagaaccg gcctccaaac catgcggttg
    13021 tccgcttgac ataagcgatt cgatgacgcg gcggtgttgg ttgaccttga tagggtaaac
    13081 caaacaataa ccgcccttat agccgcactc ttcccgtgcc gtctgaaagg cgcggttaat
    13141 gtcgcggagg cggtgttcga ggatttgcgg aaaacaaaac aaaacaggca ggcgcgcctg
    13201 atgtttttgt tgcacggctt cagtcagttt ttgcagtgaa acagtttgat tgtgttgcga
    13261 gggattgggg cggacgatga tttcgccgaa atcgtcaaca tcataataac ctatgcccca
    13321 atgattaatg ttgcacactt cacggatggt aaggataggc ataataaacc tgctccgtct
    13381 gtcgtgttga aaaggaatga ttataacaaa tcagcgtgaa atgtcatttt tttaataaga
    13441 aaagcctgcc tcatacctga tgaggaacag gcaaaatgcc gtctgaacgc ttcagacggc
    13501 attttggttc atctttccat caaaggagtc agcgatcgag ctgctctttg atgattttca
    13561 ggtcggggta agacaacacg atgtcgtatt cgcggccgtc tttataggct tccacttcca
    13621 gcacaggctt accccaatgg tcgtcggcat cgacatcgtg aacctgataa ccgcgctgct
    13681 ccaacatttt tacggctttt gtgcggtttt gttcaaaatg gggatcgccg taaatctgac
    13741 gctcggcaga atcgccggca aatgcggttg cggcactcag ggaaacaacg gcggccaata
    13801 acagtttttt cattttcagt cctttcttaa tcggttgatt gaataagatg tgtttttcaa
    13861 tgctgccatt aaaacacagc aaaattaggt ttgaattaga gggaagttaa gaagcgtaaa
    13921 tgccgtctga aaaaaacaga gccgtcaaat ggctctgttt tccttatgct tctttggttt
    13981 cagccgcctt ttgacgcagg cgcaagctca attcgcgcaa ctgcttgtcg tccacgctgt
    14041 tgggcgcgtt ggtcagcagg cattgggcac gttgtgtttt cgggaaggca atcacgtcgc
    14101 ggatagattc ggcaccggtc atcagcgtaa ccagacggtc gaggccgaac gccaaacccc
    14161 cgtgaggagg cgcaccgaat ttcaggttgt ccaagaggaa gccgaatttc tcttgttgct
    14221 cttcagggct gattttcagc gcggcaaaca ctttctcttg tacgtctgca cggtggatac
    14281 ggatagagcc gccgccgatt tcccagccgt tcaataccat atcgtaggca cgggccaggc
    14341 agtttgcagg gtcggaaacc atcaggtctt catgaccttc tttaggcgcg gtaaacggat
    14401 ggtgtacggc aacgtagcgg tcggcttctt cgtcgtattc gaacattggg aaatcgacaa
    14461 cccacaaagg tttccattcg tctgtgaagt agccgttgtc tttgccgtgt tccaagccga
    14521 ctttgatacg caatgcgccg atggcttcgt tcacgacttt ggttttgtcc gcaccgaaga
    14581 agatgatgtc gccgttttgc gcgccggtac gctcgatgat ttccttcagg gcgttttcgg
    14641 acaggaattt tacgattgga gattgcaggc cgctgtcttc gccgttggag aggttgctca
    14701 catcgtttac tttgatgtat gctagacctt tcgcgccgta gataccgaca aatttggtgt
    14761 attcgtcgat ttctttgcgg ctgaatttcg caccgttcgg cacgcgcagg gcgactacgc
    14821 ggccgccttt catatctgct gcgccacgga agactttaaa ctcttccgtc ttcatcaggt
    14881 cggtcaactc ggtaaatttc aagccgatac gcatatccgg tttgtcagag ccgtaataga
    14941 acatcgcttc ggcgtaaggc atacgcggaa aaccgcccaa gtccacgccc aacgcgtctt
    15001 tgaagacttg tttcgccata ccttcggtga tgtccataat ttcatcctcg ttcaaaaacg
    15061 aggtttccaa gtcgatttgc gtaaattcgg gctggcggtc ggcacgcagg tcttcgtcgc
    15121 ggaagcactt ggtaatttga tagtaacggt cgaaacctgc caccatcaac agttgtttga
    15181 ataattgcgg cgattgaggc agcgcgaaaa actcgcccgg atggacgcgg ctcggtacga
    15241 ggtagtcgcg cgcgccttca ggcgtggagc gggtcagcat tggcgtttca atgtcgatga
    15301 agccttgtgc gtccaagtag cggcgaacgc ccatagccac ttggtaacgc aggcgcaggt
    15361 tgcgctgcat caccggacgg cgcaagtcga taacgcggtt ggtcaggcgc acgttttcgc
    15421 tgatgttttc atcgtcgatt tggaacggcg gcgtggcggc ggcgttcaag acttcgattt
    15481 ctttggcaag gatttcgatt ttgccggaaa tcattttgtc gttggtcgtg ccttcgggac
    15541 ggttgcgtac gcggccggta atgctcaaaa cgtattcgtt gcgagcggaa tcggcagcgg
    15601 caaacgcttc gggcgtgtcg gggtcaatca cgacttggac gatgccttcg cggtcgcgca
    15661 ggtcgataaa aatcacaccg ccgtggtcgc gccgacggtg tacccagcct ttgacggtaa
    15721 cggtttggtc taagtattgc tcgctaatca gaccgcaata gttggtacgc ataaaatcac
    15781 ctttatattg atttaaactg aaaacagaaa atgccgtctg aacggcggct ttattgttgt
    15841 tcgggcaaat ccgccttttc agacggcata ggtcctgcca atgttttgac gggcaggtcg
    15901 tcaggaatga ccatacccag cgaaatcaca tatttcaacg cttcgtccac gctcatatcg
    15961 agttcgcgca catcgctttt ctttaccata atatagtaac cgccggtcgg attcggcgtg
    16021 gtcgggacat acacggaaag ataatcgccg tccttcggca atgcggcctt aaccgcattc
    16081 gacacctgcc ctgacacgaa agcaatcgtc caaatacccg attggggaaa cggcacgagt
    16141 accggcgttt taaacgagcg gctgctgtcg gacagcagcg attcggatac ttttttcaca
    16201 ctcgagtaga tggatttgac aaccggaatc cgccccaaca ggctgtccca cgcggcaaga
    16261 atctgccggc ccaatacgtt ggcggcaaat aatccggtta caaacaatac ggcaatggca
    16321 acaataacgc ccagccccgg gatattgaag cccaaaacat attgcggccg ccattgcttc
    16381 ggcagcaggt tgacgagctg atcggacgcg gaaacgatat aggaaaccac ccaaaccgtt
    16441 accgcaatcg gcagccagac caaaatgccc gtaatcagat atttttttaa cgccttggca
    16501 gctttaccgc cttcggccgc aggttccgtc atcttgcttg attccgacaa agtccgtaca
    16561 aaccgcacat tatacgcgtt tgcccggatt caaacgaaat ttttatcccg ccccgccaaa
    16621 ccgccggcgg cttcagacgg cacggcaact tgatataccg tctgaacacg cggttcagat
    16681 gccgtccaag tcgttgaaca tcaacccgat accgataccg ttctgcttgt ggttgtagtc
    16741 gatcaggctc tcgccgtaac cgtggaatcc gcgtaccacg cctttgagtt tgcccttaat
    16801 cggaaacgtg taggcggctt caatcgcgcc gtagcccgtt ttggggttgt agcgcaatac
    16861 ggaatacaca ttctgcctgt cgttcaggcg gtactgcagc ttcacgtcgc cataccccat
    16921 atagtcggca atatcgggat tgtcgttttt atcgccgctc tgatcgaacg cacgcaccca
    16981 cacgcgcgga atcaccgtca atttgcccca ttccatgcct gccatggcgt aaatcctgtt
    17041 ccacgaacgc gattcgggac ggctctgtcc gttggactgg tggacaaaac ccgcaccgag
    17101 catacgcagc ctgccgccga acggcaaatc cgccttcaca ggctgggtca ggaaaatttc
    17161 aggtttgtaa tccgtattgc ggaacggcgc ggatttcctg ccttggttgt aaatctgcca
    17221 atcggatctt tgggtgtagc cgaaccacag atccgcgcgg gttttaaaca aatcttcggc
    17281 aattttgctt ttgaacgaaa cctgcaattt ggtttccgca cgtttctgct gtccgaattt
    17341 ttcctgtaca gtcgtaccgc gcgtcggcga actcggggca tagttgggcg aattgttgta
    17401 ccagagcggc ataaggtaca tcggattgtg ttcgcgtacg cccaacagcc cgcgcaaatc
    17461 gtttttgtcc aagtcgtaca tcaggctcaa aggcgtatag atgtcggcgg tttcgcccgc
    17521 actgtcggca ggaagcgcat ccccgccttt ttcaacaaca atgaccgcct cgcccttatc
    17581 caggctgctg cggacggttt ccgtcagatt gagtacagct ttcgactcct gcccttcctg
    17641 tcctgccgaa gacggaagct gtgccgcaaa aatcctgtcg taacacgcca aacgcgtaac
    17701 attgtccgtc aaagcggcgc attgcagcgc ggtctctcca aaagcggatg ccatcggcaa
    17761 cagtcctgtc aaaagaatat agcgcatatt ccgtgtattc atctccgccc ccattgtcgg
    17821 catattggtt ttcaaacggc attttatagc ggattcggat aaaaaaccgc accctttccg
    17881 ccatttcggg atttagcccc cgtaatacag aaaaccccga gaccgtcagg cttcagggtt
    17941 ttccgcttat cgcgtatcaa ccgccttggc ggttttgcaa aaatcaagcc agtgctttta
    18001 cttttgcaga cagacggctt ttgtggcgtg ccgctttgtt tttgtggaac acgcccttgt
    18061 cggcgatgcg gtcgatgact ttgacggact cttggtaaac cgcttgtgcg gcagctttat
    18121 cgcctgcttc gactgctttc aatacttttt tcactgcagt gcggaatgcg gtacgcaggc
    18181 tagcgttgtg ggcgcgttgt ttgacggact ggcgggcacg tttacgtgct tgtgcgctgt
    18241 ttgccatatt ggaatctcct agaagaaaaa taaattctgt aaacgcgcaa ttttaaaggg
    18301 ggtttacatt atatgcaagc cttttctctc tatccgcccg tcggacaccc tgacagcgca
    18361 aaaaaacggc aaaatcgtgc ggacgttttc cccctctttt gaaaacaggg taatttttta
    18421 gggaaatcag tccaatatca ctttgagttt gcacaaattt actttacaat gcccaaacct
    18481 tgcgccgggg tgtttatggg gcgcggggtt ttcttttttt ccaacctctt tcatcaggaa
    18541 tctgcccgta tgaaaaaatc cgtattagcc gttttggccg cgctgtctct ggcagcctgc
    18601 ggcggcagcg aaaaaaacgc cgtgcaacct caagcagacg ctgcgtctgc tgccaatgcc
    18661 gaagccgctg ccaccgatac tctgaacatc tacaactggt cgaactatgt tgacgagagt
    18721 acggtcgaag actttaaaaa agccaacaat ctgaagctga cttacgactt gtacgaaaac
    18781 aatgaaacgc tggaagccaa aatgctgacc ggcaaatccg gttacgattt ggttgtgccg
    18841 ggcatcgcct tcctgccgcg ccaaatcgag gcgggcgcgt atcaaaaagt caacaaagac
    18901 ctgattccca actataaaaa catcgatccc gaactcttga aaatgctgga aaccgccgac
    18961 ccgggcaacc agtatgccgt cccctatttc tccggcgtga acacgattgc gattacggcg
    19021 aaaggcaaag agcttttggg cggcaagctg cccgaaaacg gctgggattt gctgttcaaa
    19081 cccgaataca cccacaagct gaaatcctgc ggcatcgccc tgtgggacac cccgagcgaa
    19141 atgttcccga ttttgctgaa ctacttgggc aaagacccca aaggttcgaa tcctgaagat
    19201 ttgaaggcgg cggcggaagt gttgaagtct atccgtccgg atgtcaaacg tttcagcccg
    19261 tccatcatcg acgagctggc gcgcggcgac atctgcctag cggcaggcaa cggcggcgat
    19321 ttgaacttgg cgaaagcacg ttccgaagaa gtgaaaaaca acgtcggcat cgaagtgctg
    19381 aatccgaaag gcatgggctt ctggattgag tcttggctga ttcccgccga tgcgaaaaac
    19441 gtcgccaatg cccacaaata catcaactac acgctcgacc ccgaaatcgc ggcgaaaaac
    19501 ggcatcgccg taaccttcgc ccccgccagc aaaccggcgc gcgaaaaaat gcctgccgag
    19561 ctggtgaaca cccgttccat cttcccgaac gagcaggata tgaaagacgg tttcgtgatg
    19621 ccgcaaatga gtacggatgc gaaaaaactg tctgtcagcc tgtggcagaa aatcaaagtc
    19681 ggcaccaact gatttgaagc attaaaaatg ccgtccgaac atcgttcgga cggcatttta
    19741 tattggattg aaatagaaat atttatatag tggattaaca aaaatcagga caaggcgacg
    19801 aagccgcaga cagtacagat agtacggaac cgattcactt ggtgcttcag caccttagag
    19861 aatcgttctc tttgagctaa agcgaggcaa cgccgtactg gtttttgtta atccactata
    19921 ccgtcgttcc cacggtcagg atttcagatt gcggacatct gtcagaaaag acaaaaaacc
    19981 ttccgccgtc attccccaca ggcgagaatc cgatccgttg aaattcggtt gttttaaata
    20041 aattcttgca gctttgattt tctgtttttc cgataacgcc gtaactttga aacgcgaaag
    20101 cggtaatccg atccgttggg attttgcaac ttcaaatcaa tccgcaaact gaaatcccgt
    20161 cattcccgcg aaagtgggaa tctagaacac gtccgcacgg aaacctgcat cccgtcattc
    20221 ccacgaaagt gggaatccag ttttttgagt ttcagtcatt cccgataaat tgccttaaca
    20281 ttgaatgtct agattcccgc ctgcgcggga atgacgaatc catccgcacg gaaacctgca
    20341 ccgcgtcatt cccacgaacc tacattccgt cattcccacg aaagcgggaa tccagttttt
    20401 tgagtttcag tcattcccga taaattgcct tagcattgaa tgtctagatt cccgcctgcg
    20461 cgggaataac gaatccatcc gcacggaaac ctgcacc
//
LOCUS       DAXHTY010000031        20387 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXHTY010000031 DAXHTY010000000
VERSION     DAXHTY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20387
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.031"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..452)
                     /locus_tag="ACFNMK_000936"
     CDS             complement(<1..452)
                     /locus_tag="ACFNMK_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364229.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTL"
     gene            complement(1013..1216)
                     /locus_tag="ACFNMK_000937"
     CDS             complement(1013..1216)
                     /locus_tag="ACFNMK_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364230.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
     gene            complement(1781..2719)
                     /locus_tag="ACFNMK_000938"
                     /pseudo
     CDS             complement(1781..2719)
                     /locus_tag="ACFNMK_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
     gene            complement(2722..2895)
                     /locus_tag="ACFNMK_000939"
     CDS             complement(2722..2895)
                     /locus_tag="ACFNMK_000939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364231.1"
                     /translation="MIKQVANNLPDGSPAKAAVFKAEREGKLKTAIAGVDRQTGKAVI
                     LPVKVPSKANIRR"
     gene            2945..3163
                     /locus_tag="ACFNMK_000940"
                     /pseudo
     CDS             2945..3163
                     /locus_tag="ACFNMK_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(3231..3665)
                     /locus_tag="ACFNMK_000941"
     CDS             complement(3231..3665)
                     /locus_tag="ACFNMK_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364232.1"
                     /translation="MNKNYLFYLIQNIKSIFLIGGVFFGLLIFSGLWGNDFKLSFLGV
                     IDFIFGMVGGGFIVMLWFLIPVILSTLFRWVFQNQLAVSVILTFIVFYTYGYFSLFNS
                     SERVGSFWYLVIPTIMFIGLSEWFIYKSSVHSLGENVTPRSN"
     gene            complement(3662..5344)
                     /locus_tag="ACFNMK_000942"
                     /pseudo
     CDS             complement(3662..5344)
                     /locus_tag="ACFNMK_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575200.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
     gene            complement(5720..6094)
                     /locus_tag="ACFNMK_000943"
     CDS             complement(5720..6094)
                     /locus_tag="ACFNMK_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6364233.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            complement(6113..8827)
                     /locus_tag="ACFNMK_000944"
     CDS             complement(6113..8827)
                     /locus_tag="ACFNMK_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6364234.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGEQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKSSIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTQIETLTKQPEYAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTGAGAA
                     IVVIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASLYS
                     QAAVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELF
                     SSTGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAA
                     SKIKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATL
                     SDAEKHKVISYSKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQ
                     KPDKTALEKIIQGIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGW
                     TAPLIGTAGKLAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGALSKA
                     KAAKQAAPKETINNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRKGAGLLDASN
                     IPITINGKTIKPVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVLPDGRLANGI
                     ISTGEWAGTKIALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            complement(8971..9402)
                     /locus_tag="ACFNMK_000945"
     CDS             complement(8971..9402)
                     /locus_tag="ACFNMK_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364235.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTSCSLHET"
     gene            complement(9418..12099)
                     /locus_tag="ACFNMK_000946"
     CDS             complement(9418..12099)
                     /locus_tag="ACFNMK_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6364236.1"
                     /translation="MLKTPPMLAAELSGKTGVSISAPYANENSRIILSAADIAAENGK
                     IKLQSYGDQSYYAGQGELYTLERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATVFDAPKGSINIEAGRKLTLYAVEELNYDKLDSHKKRKFLGITYD
                     KVHDTSTQQMQTALPSRVVAESANLQSGWDTKLQGTQFETTLGGAVIRAGVGDQARAD
                     AKIILEGIKSSIHTETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGLTGAGAA
                     IIALAVTVVTSGAGTGAVLGLNGAAAAATDAAFASLASQASVSFINNKGDVGKTLKEL
                     GRSSTVKNLVVAAATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNG
                     GSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIVHKIAHAIAGCAAAAANKGKCQD
                     GAIGAAVGEIVGEALTNGKNPDTLTAKEREQILAYSKLVAGTVSGVVGGDVNAAANAA
                     EVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVADKRLAASIAICTD
                     ISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQADLALQSYHLNT
                     AAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFVKQNTPITNVKYPEGISF
                     DTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQTDIEGITRIKYEI
                     PTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAASQGYSKASKIAQNERTK
                     SISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
     gene            complement(12349..12732)
                     /locus_tag="ACFNMK_000947"
     CDS             complement(12349..12732)
                     /locus_tag="ACFNMK_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364237.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(12737..18784)
                     /locus_tag="ACFNMK_000948"
     CDS             complement(12737..18784)
                     /locus_tag="ACFNMK_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6364238.1"
                     /translation="MNKGLHRTVFSKKHNTMVAVAETANSQGKGKQAGTSAPASPQIS
                     DGLYSKLKTTLKTLVCSLVSVSMVLPAHAQIITDKTAPKNQQAVILKTNTGAPLVNIQ
                     TPNARGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGTPQIGKDGALTGFDVRQGTLTVGT
                     SGWNDKGGADYTEVLARAVALQGKLQGKNLAVSTGAQKVDYASGEISAGTAAGTKPTV
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADS
                     TEASPTYLSIETTETGAAGAFISNGGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARVDAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSAPGGYIVDIPKGNLKTEIEK
                     LSKQPEYAYLKQLQVAKNINWNQVQLAYDRWDYKQEGLTEAGAAIIALAVTVVTSGAG
                     TGAVLGLNGAAAAATDAAFASLASQASVSFINNKGDVGKTLKELGRSSTVKNLVVAAA
                     TAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAINGGSLKDNLGDAALGA
                     IVSTVHGEVASKIKFNLSEDYITHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIVGE
                     ALTNGKNPATLTAKEREQILAYSKLVAGTVSGVVGGDVNTAANAAKVAIENNLLSQEE
                     YALREKLIKKAKGKGLLSLDWGSLTEQEARQFIYLIEKDRYSNQLLDRYQKNPSSLNN
                     QEKNILAYFINQTSGGNTAWAASILKTPQSMGNLTIPSKDINNTLSKAYQTLSRYDSF
                     DYKSAVAAQPALYLLNGPLGFSVKAATVAAGGYNIGQGAKAISNGEYLHGTVQVVNGT
                     LMVAGSVSAQAAISAKPAPVTRYLSNDSAPALRQALTAESQRIRMKLPEEYRQIGNLA
                     IAKIDVKGLPQRMEAFSSFQKGEHGFISLPETKIFKPISVDKYHNIASPPRGTLRNID
                     GEYKLLETIAQQLGNNRNVSGRIDLFTELKACQSCSNVILEFRNRYPNIQLNIFTGK"
     gene            complement(18877..>20387)
                     /locus_tag="ACFNMK_000949"
     CDS             complement(18877..>20387)
                     /locus_tag="ACFNMK_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFC6364239.1"
                     /translation="DDKTARKFSFLPSVLMKETAFKTGMCLGSNNLSRLQKAAQQILI
                     VRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEKRDGKSAEGSISAFNNKFPLY
                     RNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKSDLQIKWQQNKPIRFSIGIDD
                     AGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHKTDLTDATGTETASGSRSYSV
                     HYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQYQSSLAAERMLWRNRFHKTSV
                     GMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYLNRWQLDGKLSYKRGTGMRQS
                     MPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQFSYETAIHAQWNKTPLVAQDK
                     LSIGSRYTVRGFDGEQSLSGERGFYWQNTLGWHFHPRHQFYLGADYGRVSGESAQYAS
                     GKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGFQTANTVYGFNLNYSF"
BASE COUNT         4930 a         4622 c         4503 g         6332 t
ORIGIN      
        1 agagtctgta tattacattt atttttaggg tctgctagcc aatttcttgt tcccttcatt
       61 attttatctt ctgaaagaaa attatttttt tccatgctat taatattaat gatatgattt
      121 ttatttaaaa taatttttcc atataaaatt tccggtttgt aatagcaaga gtctgaagca
      181 aatccttgcc cgtcaatata agaaaaataa atgttatcat cctccttata atattctaaa
      241 accaatccgg atattatttt atcaaaattc ccttcatttc catctttcat gactaaaata
      301 taattagcag gccaaccttt atcgtaaaca tattcaaact gatagttccc gaaactctcg
      361 atatccgaac taaaaaagaa gaaaagcaaa ttaatagcca atacaaaaac aaataagaac
      421 aaaaatagca aaattttcaa cttagttaac aatatttacc tctcctttaa attcaatcct
      481 gaaaggtacc ccttaccctg ggctaccaat tatagtttcc atatttctaa atattgtttt
      541 tacattactt ttttctcccc ccaaaggaat gcattttaaa atcatgcttt tcaggtgcta
      601 atcgatactt accattaaca tctttaatca cagatatatt tccatgtata gcccaacgtg
      661 aaaaatctga gtattatata cagttatacc ttactgaaac aaagctagct gtacatatct
      721 gtaccatatt tttactacaa acaaaaaata tttataatta ttaaggataa gataaaaacc
      781 cccaacgcca gattaattct taataatttc aaataaaaaa tatttgagat tttttctttt
      841 ccaaataata tattgaaata tttaaagtta aggtagcttg atttaattag aaaatcaaaa
      901 aaaaccatta atttttcttc tttaaatatt ttatatgata ttataaaatt aaataacatt
      961 gcgaaaacat atgaaatcat caaaatacca aatatatagt gagaatgcat tattatttag
     1021 ctccttcttt tggctctgac aaattgacag tccattgata tccaatgctt gcatttgcaa
     1081 ttgctgacgc acctgcccct gttttccctg ttagtatcaa tggttctttt cttgcaagat
     1141 gtgcacctgc acaaccacct aatactaaac caccacctat tgtaaatcct tcctgttgta
     1201 tctctaatga taacattttc ttcttgtgcc gcccttaata cagattgtgg aattggattg
     1261 acctgagcag gaacttctaa aataagtttt ccttcaagct gtcttcctct tagcagatta
     1321 ggtgtacttg gaacattgga aatgacaaag tgagtgcgca aagccatctg aaggcgatgt
     1381 gtttgaacct gttgaaagcg gccaacaggc taagtgcgcc cgttgccgcc taaaaggcag
     1441 cccggatgcc tgattatcgg gtatccgggg aggattaaga gggtgtttgg gtagaattag
     1501 gaggtgttat ggggcaaaaa cagccgaaaa cctgtgtttg ggtttcggat gtcggggaag
     1561 ggcttttttg caaaggtctc aactcatgtt attgcaaaga tctcatgcct tgttttgcca
     1621 gcaggttgag ttcttctccg acaatcagcg tggtaaagcc ccgccacaac acagatctat
     1681 gtactgtgga agggcttcaa tttatagctt tcgaaaatct ttcagcttta agacggccta
     1741 aagttgtaat ctataaaaca ggagctcaga gttttacggc tcactctcgc ttcgcgttac
     1801 caaataccag cttatcggtt ttgaacgtat agtaatctat ccaatcctgc agatattcat
     1861 acgggtaaga caagtcatac tgtttcataa aatcgaagat gtcttgatat tcggctgccg
     1921 acagcggatc gtaaacaatc aaatccctcg gtgcgatttc gtggtctatg cccaaatcga
     1981 aaccgtgcat ggatgcgatt ttggcgtagg taacgatttg cggctgcagg tagaaatcga
     2041 aataggacaa tgtttctttg gcagccatac gcgcggtttt tttatcgaaa agcggctcta
     2101 aggcggcttt catcgcctcg gcatcctgtt cggcgaaagc gaggaagaag cggtaatcgt
     2161 acaaccgctt ttgcagccat ttgctagggg tggggtgcac caagactttc tcgctacgtt
     2221 gtttcaaccg atcaagctgc ttaccctcca ccatcagcag agtattgtaa atcatatgcc
     2281 ggttgaggtc gtagcggttt ataaaggctt ctgtatcgtt ggcgatgttg tcgatattgc
     2341 gcaccagaaa ctcacgcagc tgcgggctgt cgctcatcag catcagaaac atcggatctt
     2401 aaatgttgag catgtcgcag ggaaagaaga aaggcgtggg gtcagaccat cccatacttg
     2461 ccagtatctc aagtttactg taagcataag catactgttt gaacttttta aggctgtgtt
     2521 caaacagata agcatgtgag catgccgcat aaacatctat agaaacgata cagattgctc
     2581 caattggaga tccttttctt tcattaatat actctatact atcagtactt ttctctgtat
     2641 taaatctttg agtggtgcct ataattacat ctgtaatatg ctcataccat ttttgggtgg
     2701 tcatcatatt gtatcccatt gttatctcct tatattggct ttagaaggaa ctttaacagg
     2761 aagaataacg gccttacctg tttgacgatc aacgcctgct attgccgttt ttaatttgcc
     2821 ttcccgctcc gctttgaaga cagcagcttt agcaggacta ccatcaggta agttatttgc
     2881 aacttgttta atcaaagaat tctaccgaca catccaaatg gccaaaacat tgaaggcgga
     2941 aacctatttt taccgccctt accattcttg ggagaaaggg ctgaatgaga acaccaacgg
     3001 actcatccgg caatacttcc ccaaacaaac cgatttccgt aacatcagtg atcgggagat
     3061 acgcagggtt caagatgagt tgaaccaccg accaagaaaa acacttggct acgaaacgcc
     3121 aagtgtttta ttcttgaatc tgttccaacc actaatacac tagtgttgca cttgaaatcc
     3181 gaatctaagg tcatctgaaa ttaaatttag ttttcagaca atctttttct tcaattggaa
     3241 cgtggagtta cattttcacc taaactatgc acgctagatt tatagataaa ccattcagac
     3301 agtccaataa acattatggt tgggattacc aaataccaaa aactaccgac tctttcagat
     3361 gaattaaata aagaaaagta cccatatgta taaaaaacaa tgaaggttaa tatgacagat
     3421 acagcaagtt gattttgaaa tacccatctg aataaagttg ataatatcac tggaattaga
     3481 aaccataaca taactataaa cccaccccct accatcccaa atataaaatc tattacccct
     3541 aaaaaagata gtttaaaatc attaccccat aaacctgaaa aaattaaaag gccaaaaaat
     3601 actccaccta ttaagaaaat agatttaata ttttgaataa gataaaataa ataattttta
     3661 ttcatggctt gcatccctgt ttctcagaat atcccataag cttacaacca tatttaattc
     3721 tagatttgta atctaacata ttaattccat cctctatttt tttcctctca tctaaaggat
     3781 ttggaattat tgaaaatatc ttatttgatg ttaccccttt aatcaatgac aaataatcct
     3841 tctcattact caaatatttt tggtgaatag gctgtaataa cttttgctcg cttttcaact
     3901 gttgaatatc ccactcccta ggattagttt ttttattgaa caagtctcta aaattataaa
     3961 atccatttac tatcagtgca ttgcctgacg cagccctcca ttcataaatt ggaggattac
     4021 caacaacttt acctgccgca aaagttatat aagaagcttc tccgtctgcc cctaacccag
     4081 ttatagcagc acgcgataca aaatcagctc caccaaacca tcgtgctttt gaacccaaat
     4141 tttgctcata aagattgctg gcagcaaaga aatctgcacg attgtcaatg gtgttgaaat
     4201 acttgtcaaa tcttgtagat gccccttgtg agttatataa atagccaaaa acttctttgg
     4261 caacccgtga tacatccgta ggcatatact tccacgcagc aggcatggca atatatttaa
     4321 tagacatatt aatgccgttt ctgcaaccat cgcccaatgg gttcctagaa cagacttttt
     4381 gaatttccgt atcaattact ttgctaaatt gttcatcctt tttgatggca tttacttttt
     4441 ccatcgtagt agaacaagtt ttaccggaaa ggcattttgt caatttatcc attctttcct
     4501 tgctaagcgc attactctct accgccacag cagccgcatt cgccgcagca ttcacatccc
     4561 ccttactcaa cgccgcaacc gtccctgctg ccagcttcgc cttagcaatg atttttgccc
     4621 ggtctttcac attcaggctg cccgggtctc tgccgtccag cagggcttcg ccgaggattt
     4681 caccgaccgc cgctcctatc gcaccatcct gacacttgcc cttattcgcc gccgctgccg
     4741 cacagcccgc tatggcatgg gcaatcttgt ggactatgta gtgctgatcc aactgtttga
     4801 ttttactggc tgcttctcca tgcgcggtat tgaccaaagc cgcaaggata ttcgcttcca
     4861 aattgtcttt caggctgccg ccgttgacag cggtattaat cagtgcggca ctgcccgcat
     4921 tggccaggtt gacgttgagg ttgtttaccc aaggggtttc gctccaagtg gcaagggagg
     4981 aagcaccgat tttgttggat acgcctgcga tggccgctgc tacaaccagt tttttcactg
     5041 tgcggcttct gcccagctcc ttcagggttt taccgacatc gcctttattg ttgatgagcg
     5101 atacggaagc ttggctggcc agcgaggcga atgcggcatc ggttgccgct gcggccgcgc
     5161 cgtttaagcc cagtgcggct ccggctcccg cgcccgcagt aaccacggta acagccagcg
     5221 caataatcgc tgctccggct ccggttaagc cttcctgttt atagtcccat ttgtcgtacg
     5281 ccagttgtac ctggttccag ttgacgtctt tggtgacttg gagctgtttc aaatagcaag
     5341 cgtaggaggt gtcttcagca cgcacgcgtc tgggcagtat atagggaatc tgaatattta
     5401 cttgcataac aaatgccgtc tgaaaaaatt gagcttttca gacggcattg agccgtaaat
     5461 catggaacgc gtgcgcgctg aagcacacac cttacgcatg gattttaggt ttcatgcagg
     5521 ctacagcttg cttccataaa tcatttttat cagagctcgt aggtacggtt agccgccttt
     5581 agcggcgtaa ccgtacgaat gaaatgccaa gttgcaaggc cgtctgaaaa agttgaaaaa
     5641 cagatttcag acggccttgt tattttataa agtttgctga tatgcgtacg gttacgccgc
     5701 taaaggcggc taaccatacc tacgcttgct cataaatatc aatattcggc aaatcggcca
     5761 aatctattgg acacgcaata tcccaccaaa gccattctaa gtaataccaa gggtcttcag
     5821 gccatattgc ttgggcatct tccaaagtag gccatatgtc tttcaatttc tgcacttgtt
     5881 cttttgaagt tccagttaga ggaattccga taccgtcggt ataatcatgt aaacggattg
     5941 aaccgtcatt taacagttct tccataaaag aacagaaatt gttttttaag tttttatctt
     6001 gaatattaat acccatttga tttttataag agttaaaaat tgaggataaa tcgatttgaa
     6061 aatcaagaat cttaattttt tgtttctcat ccacggtaaa atcctttgta aattattgga
     6121 atttaagctc caatttagta cctttaataa atgatggagg attctgcaaa tctaatgtcc
     6181 atcgtgcttg agttgaattc tctgtttttg aaaaatttct taatgcaatt tttgttcctg
     6241 cccattctcc agtactgata atgccatttg ctaatcttcc gtcaggcaaa actctaaaat
     6301 tcggattctg gccagtcatc tgccgataaa gcgcaaaaat ctcctgttcg cttacgccgc
     6361 tgtaaacggg tgctcccttt aagcttatgg cttgtacagg tttgatggtt tttccgttaa
     6421 tggttatcgg aatattactt gcatcaagca agcctgctcc tttccttact ctattgttaa
     6481 atcccgtctt ccatgcatcc agttgcgtat cgcgttgggc aatacggaat aaaatctgct
     6541 gttctgcttt ggctaaattt gccaaattgt ttattgtttc tttaggagca gcttgcttag
     6601 ccgccttcgc tttcgacaac gctcctactg gagcttccca agcgttgcct accgttaccg
     6661 cacccgtggc gattcccgcg cccgcttcgg cagcctgagt gaccatgaca gtacaaccag
     6721 aaggattagc catgcaggtg ctgatagcta atttacccgc tgtaccgatc agcggagctg
     6781 tccaacctgc tgcataaacc ccatagctgg taatcacaat cgggcctgtg atgccattac
     6841 ggatattgct tatccaaatg gcagcatcct tatcctgcgg attagtcatc gcacctgctg
     6901 catgtgcagg cataatacct tggataattt tttccagtgc ggttttgtcg ggcttctgcg
     6961 gttgatgctt tttcgcattg gtaggggtgc tgtcaaaatt caaagcatta ttcaccacag
     7021 ctacctcagc cgcattcgcc gcagtattca catcgccgcc gttgagtgcc gccacgctgc
     7081 ccgcaataat cttcgagtaa ctgataactt tatgcttttc tgcatcgctg agtgtagcag
     7141 ggtttctgcc gccaagtatg gattcagcta cgatttcccc aactgctgcg ccaattgccc
     7201 catctttaca ttttccctgt accagtccgc taacacaacc cgccaaagcg tgggcgaact
     7261 gtttggcaac ataatcgtcg ctgaaggttg ttttgatttt gctggcggct tccccttgga
     7321 agctattaac caatgctccc aatgcggcat tgcctaagtt gtctttcaag ctgccgccgt
     7381 taacggcggt attgatacca gctgagatac ctgcattgct gaggttggtg gccaatctgc
     7441 ctccaaggtt ggcaatggtt tgattgcccg tactgctgaa cagttcggtt cttaccttgc
     7501 tgttcaattg ggcaatatct gctcccatct ggtttaatgc acccgccgtc agtgcggaag
     7561 taacaatctg cttgaccgta tcactggtgc cgagatcttt taatgctttg cctacatcgc
     7621 ctttattatt gatgatggct acagctgctt ggctatacaa ggaggctaaa gctgcggttt
     7681 gcatggcagc tgcagtagaa acagtggtag ttgctgccgc tgtcgttgtt gcagctgcgg
     7741 ctgtactact tcctgcggca gttacgctac ccgctgcagc cgctccgtat ccataagtta
     7801 gagcggttac aataatcacc acaatcgctg caccggctcc ggtcaagcct tcctgtttat
     7861 agtcccattt gtcgtaggcc agctgcacct gtttccaatc gacgttcttg gccgtctgaa
     7921 gctgtttcag gtaggcgtat tcgggctgtt tggtgagggt ttcgatttgg gttttcagat
     7981 tgcctttggg aatatcgaca atgtaaccgc cgggtgcgga cagtacgggc gcaacgggac
     8041 cggtgaaact cggcaattgc aaggtttcga tgttactgcc ccgtcctgcc tgtttttgcc
     8101 atagagtaga tttgctgctg ctcacggttt ctgtgtggat gctgcttttg atcccttcga
     8161 ggataatctt ggcatctgcc cgtgcctgct cacctacgcc tgcgcgtatg gttgcgccac
     8221 ccagtgtggt ttcaaactgt gtgccttgca gtttggtatc ccagcccgat tggaggttgg
     8281 ctgattctgc aactaccctt gagggcagcg cggttttcat gacttgggtg gtggtgtcgt
     8341 gtgctttgct gtagctgatg ccgagaaatc tgcgcctttt ttggctgtct agtttgtcgt
     8401 agttgagctc ttctacggca tagagtgtca atttccgccc ggcttcgatg ttaatgctgc
     8461 ctttgggggc atcgaatgcg gtggcgtagg cgtcgatgct gccaccagat ttgatgtcga
     8521 tgccttggga tgcgctgagg tttactgcgt cgggcttggc gtttttgtgt tctttgactt
     8581 cggtaatgtg tttgcggttg taccatttgc cggttttgta gctgcggcgt tcaaaggtat
     8641 agagttcgct ctgtctcgca tagtaatatt gttcaccgta agattgaatt ttgattttgc
     8701 cgttttccga actgatatcc gtggtgctga gcaggatgcg actgttctca ttggcatacg
     8761 gtgcgctaat gctcacaccg gttttacccg aaagttctgc agcaagcgta ggaggtgtct
     8821 tcagcacgca cgcgtctggg cagtatatag ggaatctgaa tatttacttg cataacaaat
     8881 gccgtctgaa aaaattgagc ttttcagacg gcattgagcc gtaaatcatg gaacgcgtgc
     8941 gtgctggagc acacacctta cgcatggatt ttaggtttca tgcaggctac agcttgtttg
     9001 agaatatgtt ttttgtattt ctttacactt tttttgatat tcagggtgag ctattaagaa
     9061 atcagatatt gcaatattaa agtaatgata tgctaaatta ataggtataa ttaattcttc
     9121 aggattgaaa tcatctccaa ctgaaaaata aatagaagga gctttatcaa tatactcaaa
     9181 tccatttacc tcttcataat ctccaatcca tctggtagca tctatttttt catattgagc
     9241 acctattact ccaaaatgtc cggtagttgt aatattttta aaacactcta aaagcgtatc
     9301 tttagtttca aattggtaaa ttgtttcaaa aaaaatcgaa ataacaaaat ggttgttatt
     9361 tatagatttt ttatttaagt ttagaaaaat attatttttc ataatttttc ctttaaatta
     9421 ttctgggtga atgtttgtaa ttcttcctgt atttacatca aaatatgatc taaatttgat
     9481 tccgtcaaag gtttctgaga attgaatgac attttttctt tccgatattg atttagttct
     9541 ttcattttga gcaattttag aggcttttga atatccttgt gaagcagcat tttgagccat
     9601 ttgaagtatt ttatcatcag aaaatttttt aggattataa acagttttta tacttgaaat
     9661 ttccttaaat ccaccatcag gtttacctgt cctgtctagt gtaggaatct catatttaat
     9721 tcgggtaatg ccttcaatat cagtttgggt ttcagatttt acgcgtcctc ctcgtgaatt
     9781 tagttctgcc ataaaattgg tgcggttatg ggctccttta atgccctgtt tttgactaaa
     9841 accatcagca tttgccagat gtctttttag gtttgtatcg aaactgatgc cttccgggta
     9901 tttgacatta gtaataggtg tattttgttt tacaaaccct cttggtattg gaatgaatct
     9961 aggattaact cctgaaataa gtgtataacc ttggtcggac atccattcgg ataaaggctt
    10021 tgtattgccc gattgaagcc aagatttagc agcagtattc aaatgataag actgtaaagc
    10081 caaatctgct tgggtgtaag atttgctgaa taatttatac cattcatcat ctttaccaat
    10141 tagacctgct tcccaagatg aatgaaggct tctactatcg atcaaatgtt gttttctgat
    10201 tgttctacat tcagtactac gggatatatc cgtacatatt gcaatcgaag cagcaagtct
    10261 tttatcagca acattttgat acttttttac agtatttttt ctgcacagtt gaggattatt
    10321 ctgtttggcg catgcagtca tttcgttatc aaattctcta ccctctttgt cgctaagctg
    10381 attatttttc accgctacct cagccgcatt cgccgccgca tttacatcgc cgccgaccac
    10441 accgcttacc gtaccggcaa ccagtttgct gtatgccaaa atctgttcgc gttctttagc
    10501 tgtcaaagtg tcaggatttt tgccgtttgt caaagcctcc ccgactatct cgcccacagc
    10561 cgcacctatc gcaccatcct gacacttgcc cttattcgcc gccgctgccg cacagcccgc
    10621 tatggcatgg gcaatcttgt ggactatgta gtgctgatcc aactgtttga ttttactggc
    10681 tgcttctcca tgcgcggtat tgaccaaagc cgcaaggata ttcgcttcca gattgtcttt
    10741 caggctgccg ccgttgacag cggtattaat cagtgcggca ctgcccgcat tggctaggtt
    10801 gacggtcagg ttgttgatcc actgcttatc gctgacattg ttcagtgccg aagcgccgat
    10861 tttgtcggct acgcctgcgg tagcggcggc aaccaccaga tttttcaccg tgctgcttct
    10921 gcccagctct ttcagggttt tgccgacatc gcctttgttg ttgatgaacg atacggaagc
    10981 ctggctggcc aaagaggcga atgctgcatc ggttgcggcg gcggccgcac cgtttaatcc
    11041 caatacggct ccggttcctg cgcctgaggt gaccacggta acggccagtg cgataatcgc
    11101 cgcacctgct ccggttaagc cctcctgttt gtagtcccat ttatcgtaag caagctgcac
    11161 ctgattccag ttgatgtttt tcgctacttg aagttgtttc aaataagcat actcgggctg
    11221 cttggcgagt ttttcgattt cggttttcag attgcctttc ggaatgtcga cgatgtagcc
    11281 gccgggagca gagagtacgg gcgcaacggg gccggtgaaa cttggcagtt gcagggtttc
    11341 gatgttgctg ccgcgtcctg cctgtttttg ccagagggcg gatttgctgc tgcttacggt
    11401 ttctgtgtgg atgctgcttt tgatcccttc gaggataatc ttggcatcgg cccgtgcctg
    11461 gtcgcctacg cctgcacgga tgaccgcgcc gcccagcgtg gtttcaaact gcgtaccttg
    11521 taacttggta tcccaacctg attgcagatt agctgattca gccactacac ggcttggtaa
    11581 ggcagtttgc atctgttggg tagaggtatc atgtacctta tcataagtaa tgccgagaaa
    11641 tttgcgtttc ttatgactgt ctagtttgtc gtagttgagc tcttctacgg catagagtgt
    11701 caatttccgc ccggcttcga tgttaatgct gcctttgggg gcatcgaata cggtggcgta
    11761 ggcgtcgatg ctgccaccag atttgatgtc gataccttgg gatgcgctga ggtttactgc
    11821 gtcgggcttg gcgtttttgt gttctttgac ttcggtaatg tgtttgcggt tgtaccattt
    11881 gccggttttg tagctgcggc gttctaaggt atagagttcg ccctgtccgg cgtaatagga
    11941 ttggtcgccg taggattgca gtttgatttt gccgttttct gcggctatat cggcggcact
    12001 caaaatgatg cggctgttct cattggcata cggtgcgcta atgctcacac cggttttacc
    12061 cgaaagttct gcagcaagca tagggggtgt cttcagcacg cacgcatctg ggcagtatat
    12121 agggaatctg aatatttact tgcataacaa atgccgtctg aaaaattgtg agcttttcag
    12181 acggcattga gccgtaaatc atggaacgcg tgcgtgctgg agcacacacc ttacgcatgg
    12241 attttaggtt tcatgcaggc tacagcttgc tgcggcggcg gtataaaagt atttttagtg
    12301 atttaccgtt tttggtaatt ttgcccgacg gggttaaaaa tacagttgct actcttgctg
    12361 catgatattg gcaagattta atttaaatgt ttgttgaatc ttgtttacat ccttagcaaa
    12421 aatatgatag tcactcgaag aatagataga ttttattcta tccaaattag tttgttgata
    12481 acattttttc atttccgcga tgaaaaaatt atcttttcct ctttcattga gacagttatt
    12541 ttctaacaga tagattgccc atgctgtaaa aataaatgta ctatcttccg gatattcaga
    12601 taaataaatt tcgaattcaa ttatagaata ttcagctgct tcaattaatg ttatatctaa
    12661 atctctgaca aatttttccg ttatattttt aaataatttc ttgaattcta tataattttt
    12721 tattttttcc atgattctat tttcctgtaa aaatatttaa ttgaatattt ggatagcgat
    12781 ttctaaactc taaaataaca ttgctgcaag attgacaggc ctttaattct gtaaatagat
    12841 caattctacc tgatacatta cgattatttc cgagttgctg tgctatagtt tcaagtaatt
    12901 tatattctcc atctatattt cttaatgttc ctctaggagg agaggcaata ttatgatatt
    12961 tatcaacaga tataggttta aaaatttttg tttcaggtaa cgaaataaat ccatgttccc
    13021 ctttttggaa agaactaaat gcttccatcc tttgcggtaa tcctttaaca tcaatttttg
    13081 ctatcgcaag attccctatt tgtcgatact cttccggcag tttcatgcgg attctctggc
    13141 tttcagcagt taaagcttgt cttaaagcag gagcactgtc attgctcaga taacgggtaa
    13201 caggtgcagg cttggccgat attgcagcct gtgcagatac agatcctgca accatcaatg
    13261 tgccattaac aacctgaact gtaccatgca gatattctcc attagagatt gctttcgctc
    13321 cctgtccaat gttatatcct cctgctgcca cagtagctgc tttgacactg aagccaagcg
    13381 gtccgtttaa taagtaaagt gcaggttgtg cggcaacagc tgatttgtaa tcaaaagaat
    13441 cataacgact caatgtttga taggctttcg ataaggtgtt attaatatct ttggaaggaa
    13501 tagtgagatt acccattgac tggggcgttt tcagtatcga agctgcccaa gctgtgttac
    13561 ctccagaggt ttggttaata aaatatgcaa gaatattttt ttcttgatta tttaaactac
    13621 ttggattttt ttgatatcgg tcaagcaatt gattagaata tcgatctttc tcaatcaaat
    13681 agataaactg ccttgcctct tgttcggtca ggctgcccca atctaaagat aataggcctt
    13741 tccctttggc ttttttgatc aatttttctc taagagcata ctcttcttga gataataggt
    13801 tattttcaat cgcgacttta gccgcattcg ccgctgtatt cacatcgccg ccgaccacac
    13861 cgcttaccgt accggcaacc agtttgctgt atgccaaaat ctgttcgcgt tctttagctg
    13921 tcaaagtggc aggatttttg ccgtttgtca aagcctcccc gactatctcg cccacagccg
    13981 caccgatcgc accatcctga cacttaccct tattcgccgc cgctgccgca cagcccgcta
    14041 tggcatgggc aatcttgtgg gtaatgtagt cttcgctgag attaaatttg attttgctcg
    14101 ctacttctcc gtgtacggta ctgactatcg cacccagtgc ggcatcgccc aagttgtctt
    14161 tgaggctgcc gccgttgatg gcggtattaa tcagtgcggc actgcccgca ttggctaggt
    14221 tgacggtcag gttgttgatc cactgcttat cgctgacatt gttcagtgcc gaagcgccga
    14281 ttttgtcggc tacgcctgcg gtagcggcgg caaccaccag atttttcacc gtgctgcttc
    14341 tgcccagctc tttcagggtt ttgccgacat cgcctttgtt gttgatgaac gatacggaag
    14401 cctggctggc caaagaggcg aatgctgcat cggttgcggc ggcggccgca ccgtttaatc
    14461 ccaatacggc tccggttcct gcgcctgagg tgaccacggt aacggccagt gcgataatcg
    14521 ccgcacctgc ttcggttaag ccctcctgtt tgtagtccca tctgtcgtaa gcaagctgca
    14581 cctgattcca gttgatgttt ttcgctactt ggagctgttt cagataggca tactcgggct
    14641 gtttggacag cttttcgatt tcggttttca gattgccttt cggaatgtcg acgatatagc
    14701 cgccgggtgc ggacaatttg ggcggagtag ggctttcgaa gctgggcagt tttagcgttt
    14761 cgatagtgct gccgcgtccg gcctgtttct gccatacggt tgagttggtt tctaattttt
    14821 cttccgactg gatacggttc acaatgcctt tgaggataat tttcgcatcg acacgggctt
    14881 tttcgcctac acctgcctga atgtcggcac cggccagcgt ggttttgaat tcggtacctt
    14941 cgagcacggt atcccagcct gaacgggtgg ctgcagtttg ggcgacgacg cggacaggca
    15001 atttggtttc gttcagttcg tttttactgt aattgctctt acctaccttg atgccgataa
    15061 agcggcggct tttttggaca tccaactcgt gcttgtggat gccttcttct gccagcagtt
    15121 gcagctcttc acccgcaacc agggtaactt tacctgcagg ggcattgaag cgggtggtat
    15181 tagcttcgat gttgccgcct gcctgaagcg tgataccgtt ggcggtcagc tcgacggggg
    15241 ctggcataat caggtggtcg cgggtgctgg taaacttggt ttttctgatg attttgccgc
    15301 ttttaccttt ggtttttaag aaggtatagg catcgttttg tccagcctcc agtacaatat
    15361 cactatgggc tttgatgtct atgctgcctg agggagcttt gatttcggat gcaccgataa
    15421 taatacgtgc atcatcgagt gccgcagctg catgaatact tacccccgta cgtccggtca
    15481 aacgtgaagg cttgttcaga gcagctttgt cgtagtgact cttgtaggtg ggcttgccaa
    15541 tttcatattg gtcggttatg ccgtcaatca gaatagcagc cgcctctgaa tctgctgcct
    15601 ttggcaatac gcctgcggcg tgaaggttca gttttttgga agcggtaata tcggaaccgc
    15661 tgatttcgat gccttgtgcg gaaatcaagt caatattttg tgcagaaagc ttggcttgca
    15721 ggtattcttt gcctttgggt tttttacctt taacttcctt gttgatggct tgaatataga
    15781 aagcgagacg gtcgcgttct tcttgcaggg ttggaatcag cttgctttta ggcgagcttt
    15841 ttttcaactg cgcaatctgc tgttccaatt ctttggattt ttggttgagt tcagccgctt
    15901 tttgtgtagg aaaataattg ctgaatgagt tgtttacggc ttcgatattc aacttgcctt
    15961 tggtggtggc gacaaccaag tttttaccgg ctgtaatttt agaacctctt aaatctgttt
    16021 ctcctgtaac cagacggata ttgccttttg cttccaatga ggaaacttga gcactaggcg
    16081 cacctgcatt tcctcctttt gcagacaaca gcaattttcc gcctgttttg atgctcaggt
    16141 cggtatgcgc actgatgcgg ttggcaggtt cgatggttaa tgtgccgcta cctgcttcaa
    16201 tattcagccg tccggccaat ggttttaatt cggcattatc ttccaaagtc ttggtcgaaa
    16261 cggtactcca attgatgttg ccgcgcttga ctagggcggt accggcagta aggttgattg
    16321 cacccgctct cagcgtggtg ttgtcggcaa tttgggaata gcgcgcattg agtgccaata
    16381 caccgttagc caccagcttg ttggcagaag gcagtttgtc gttttgccaa atctggctgc
    16441 cggtaatgct tagatgacgg tgtgcgtagg catctacttg gttgagcgtt acccgctcgt
    16501 gttgtgcatt aagatgcgta ttatgggtag actccagctt ggtattttct atgctcaatg
    16561 cccggtcgga atgaatgttc aatgccccgc ttttggcatg gacgttaagg ttttttaagt
    16621 cggcattacc accgttgttt ttgatgctga tgtgttttcc gttgattgaa ttgcgttgtt
    16681 ttccgtcacc aagctgaata ccgttgccgg caaccaacgt aatatctcct gaagatgaag
    16741 tgatattggt attgtctgct ttcagacggc ctttaccaac cgatcctgca ttgacatcgg
    16801 ccttggctgt cagggtattg tgaccggtaa agtcggcatt accgttggcc aataaggata
    16861 catgaccgtc tgcagaaaca gcatgaaggc cgtctgaaac gatattgccc tgcaatgcgg
    16921 tggtttcgag agccttggct gcgttcagct tggtattgcg aagctgaata ttgcctttgg
    16981 ctgcctgaat gtgcagatta cccgagttgg tacgcagatt ggtattggta acattcagcg
    17041 agcctgcctc cacacccatg tcttttgagg cagtgagggt tttactggtg ccggtaatat
    17101 gggcagcgtt atccgatttc aaatggatgc tggcggcaga caaatcttta tcaacattca
    17161 aattcagatc tttacctgta tgaacataca gattgccggg agtattcaga ttggtagttt
    17221 tggcagtaat gttatcgtct gccagtaaag caagctgctt gccgcccttg atactgcctc
    17281 cgtttaagcg gatatcggag gtaactgttg aagcttccaa agaaagcggt ttgcctgctt
    17341 cgatgtgtgc ggtgtctttg gcatctatta cggcggaact gctgatggtg ccgttggata
    17401 atacggtaac atctgccccg gtaatgcgtg tgttattgcc taattcggcg ttgcctttgc
    17461 tggaactgta tacggtagtg ccagtctgaa tactggcctc cttgatgacg gtacggccgc
    17521 cggtcgacag attggtcgaa cctttagcat tgttcacatt tgctttgctt tcgataacca
    17581 aattacggcc ggcatttaat accgtagcag cagggcggtt gccgttgttt tgaacgaccg
    17641 ctccgttacg caaaccgatg tcctccccgg tatcaataac caataagcct ttgctctcta
    17701 tccggccgcc attggagata aatgcgcctg ctgcgcctgt ttcggtggtt tcgatggaga
    17761 gataagtcgg tgatgcttca gtgctgtcgg cagtggtggc gatgcggccg ctgttttcaa
    17821 tacggccgga ggaagtaacg atcaattgct tggccgcttc gagtgtgccc gcatttttga
    17881 cacccacgcc tttttcattg gcaatcaggg taatgctgtc ggcgtacata ccacccaatg
    17941 cggcagtatc gagggcaacg gtcggtttcg tacccgctgc cgtacctgcg ctgatttcac
    18001 cgctggcgta atctactttc tgtgcgccgg tcgaaaccgc caggttttta ccctgcaatt
    18061 tcccctgcaa agcaaccgca cgggcaagca cttcggtgta atcggctccg cctttgtcgt
    18121 tccaacctga cgtaccgacg gtcaatgtgc cttggcgcac gtcaaatccc gtcaaggcac
    18181 cgtctttgcc gatttgaggc gtaccggtgg ttaagatacc gcggccgacg tttttaaagc
    18241 cgccgccgtt cacggtaatg ccgttggggt tggcgataat cacatcggcc ttttgaccac
    18301 ctacggtaac gatgccattg agtttgctgg ccgcaccgcg cacctcgttc aaaatcaact
    18361 gcgcactgcc tttggccaga aacgggttgt tgttgcgatc gttgttcagt acagcacctt
    18421 tagcgtcgac atcaaactgg gtatagcggt tgtggctcaa tccacgggca ttaggagttt
    18481 ggatgttgac caacggcgca ccggtattgg ttttaagaat aacggcctgc tggtttttgg
    18541 gcgcggtttt atcggtaatg atttgtgcgt gggcaggcaa taccatactg acggaaacca
    18601 aagagcagac caaggtttta agcgtggttt tgagtttact gtaaaggccg tctgaaattt
    18661 gtggcgaagc cggagcggaa gttcctgcct gtttgccttt tccctgactg ttggcggttt
    18721 cggcaactgc aaccatagtg ttgtgctttt tgctgaaaac ggtacgatgc agacctttgt
    18781 tcatatttat ccctttaaat gatgtttgta atcgcttcag acggcctgta aatgatgaaa
    18841 gactgtctga atgtcggtaa aaaatgcagg agagggttag aaactgtaat tcaaattgaa
    18901 gccgtaaacg gtgttggccg tctgaaaacc ttcgggcttg tgcagcggct tgccggcaaa
    18961 taagtcataa gcaaatatac cgcctacttt atggctgcct ctgaagccgg ccactgcacc
    19021 cgccagccgc ttgcccgagg catattgggc actttcgccc gatacccgac catagtccgc
    19081 gccgagatag aattgatggc gcggatggaa atgccagccc aaagtgttct gccagtagaa
    19141 accacgctct ccggaaaggc tctgttcacc gtcaaatcct cgaacggtgt agcggctgcc
    19201 gatagacaac ttgtcttggg caaccaaagg cgttttgttc cattgtgcat ggatagcggt
    19261 ttcgtaggaa aactgctgtt tgcccaacat aaacggggcc gctgcatcca atccggcggt
    19321 tatgattttc atacgggatg tgcctggaat agtaccgccg ccgttttctt caggtgcggg
    19381 catactttgg cgcataccgg taccccgttt gtaagataac ttgccgtcca gttgccagcg
    19441 gttgaggtaa gcacggtggc gcaattcggc ttcccagcct gcagagcggc ggcgttgcac
    19501 ttcgatttcg gcatcgtcga tgtatttata ggtttggcgt gtccataatt tcattccgac
    19561 tgaagtttta tgaaacctgt tacgccaaag catgcgctcg gcggccaggc tgctctgata
    19621 ttgtttgccg ttgtaatcgt aattgacgga atagccttcg gttgcttcgt ggtaacgatg
    19681 tccattgtga ttaaaagaaa acagccattt ttttacgggc accgaataat gcacgctgta
    19741 acttctggat ccgcttgcag tttccgtacc ggtggcatca gtcaagtccg ttttgtgcgc
    19801 caaaccgcgt ccatatgaaa cataaaacaa atcgcttaag cccaaagggt tatcgaacga
    19861 taaagcgaca tttccttgat atttgccggt cgttttgccg cccgcatcat ctataccgat
    19921 actgaaccgt atgggtttat tctgctgcca tttgatctgt aaatcgcttt tgccttcttc
    19981 ttcggacggt ataatctgaa tatctgtttt aacactcgac aaacgacgca ggttttccaa
    20041 gccctgctct acatcgcgaa gattgagaat tttgttccta tataagggaa atttgttatt
    20101 gaatgcacta atactgccct cggcagactt cccatcccgt ttttcttcat agcggatatc
    20161 ccctatttcg cctgctgata cccgtaattt cagaattccc gaatccatat tctgtggttg
    20221 gataatagct tgggaagtga ggtagccacg cacaatcagt atctgttgcg cggctttttg
    20281 tagcctgctc aaattattgg aacctaaaca catcccagtt ttaaaagctg tttctttcat
    20341 gagcacagaa ggaagaaaag aaaatttgcg cgccgtctta tcatcta
//
LOCUS       DAXHTY010000032        19665 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXHTY010000032 DAXHTY010000000
VERSION     DAXHTY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19665
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.032"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..2318)
                     /gene="pilC"
                     /locus_tag="ACFNMK_000950"
     CDS             complement(<1..2318)
                     /gene="pilC"
                     /locus_tag="ACFNMK_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6364240.1"
                     /translation="MNKTLKRQVFRHTALYAAILMFSHTGGGGAQAETHNYAIVMNEQ
                     NQLKVKQEGSYSTLREKDRERKFIYNRDRQGGGSVFFDNTDTLVSRQSGTAVFGTATY
                     LPPYGKVSGFDERKLKERNNAVDWIHTTRAGLAGYAYTNVICRSSDQCPQLVYETKFS
                     FDGIGLVKNAGRLDRHSDPSRENSPIYKLKDHPWLGVSFNLGSENTVKNGNSFNRLIS
                     SFSEDNNNQTIVSTTRGHLISLGDQQREHTAMAYYLNAKLHLLDKKGIKDIAQGKTVD
                     LGTLRPRVEAKVRRGGNLLNFWATWKIEDKGNITVRLDLPEVKAGRCTNAAHPNSKAK
                     PPSPALTAPALWFGPVQNGKVQMYSASVSTYPDSSSSRIFLQNLKRKNDPNKPGRYSL
                     ADLSASDIQSKEPTFTSRQTVIRLDKGVHQIKLQGNEVTGFNGNSNNATFGIVSEGSF
                     MPDASEWKKVLLPWTVRASNDDGQFNTFNKKENNGKPTYSQKYRSRDNSKRERDLGDI
                     VNSPIVAVGGYLATSANDGMVHIFKKGNGDARNYSLKLSYIPGTMPRQYFDNDTSALK
                     DSTLAQELRTFAEKGYVGDRYGVDGGFVLREVERGGKKHVFMFGAMGFGGRGAYALDL
                     TKVDSNNPTAVSLFDVKNDNNNGNNGVKLGYTVGTPQIGKTHNGKYAAFLASGYATKD
                     INSNDNTTALYVYDLENNNGTPIAKIEVKDGKGGLSSPTLVDKDLDGTVDIAYAGDRG
                     GNMYRFDLSGNNPTKWSARAIFSGNKPITSAPA"
     gene            complement(3248..4327)
                     /gene="apbC"
                     /locus_tag="ACFNMK_000951"
     CDS             complement(3248..4327)
                     /gene="apbC"
                     /locus_tag="ACFNMK_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC6364241.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPETGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIAKIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
     gene            4538..4613
                     /locus_tag="ACFNMK_000952"
     tRNA            4538..4613
                     /locus_tag="ACFNMK_000952"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4571..4573,aa:Lys,seq:ttt)
     gene            4692..5201
                     /locus_tag="ACFNMK_000953"
     CDS             4692..5201
                     /locus_tag="ACFNMK_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC6364242.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            5544..5984
                     /gene="nadR"
                     /locus_tag="ACFNMK_000954"
     CDS             5544..5984
                     /gene="nadR"
                     /locus_tag="ACFNMK_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC6364243.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            6099..6599
                     /gene="hpaC"
                     /locus_tag="ACFNMK_000955"
     CDS             6099..6599
                     /gene="hpaC"
                     /locus_tag="ACFNMK_000955"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC6364244.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRKARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            6615..7310
                     /gene="murU"
                     /locus_tag="ACFNMK_000956"
     CDS             6615..7310
                     /gene="murU"
                     /locus_tag="ACFNMK_000956"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC6364245.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(7555..7986)
                     /locus_tag="ACFNMK_000957"
     CDS             complement(7555..7986)
                     /locus_tag="ACFNMK_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6364246.1"
                     /translation="MGSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTDRNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            8161..9837
                     /locus_tag="ACFNMK_000958"
     CDS             8161..9837
                     /locus_tag="ACFNMK_000958"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC6364247.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLADLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGVGGADLARKVVNAIENQPNNFNFSYDVELSIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPVCMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            9952..11043
                     /gene="ychF"
                     /locus_tag="ACFNMK_000959"
     CDS             9952..11043
                     /gene="ychF"
                     /locus_tag="ACFNMK_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC6364248.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVELCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LERLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            11313..11513
                     /locus_tag="ACFNMK_000960"
     CDS             11313..11513
                     /locus_tag="ACFNMK_000960"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6364249.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTAILLREMSGGGGGFP"
     gene            11546..13192
                     /locus_tag="ACFNMK_000961"
     CDS             11546..13192
                     /locus_tag="ACFNMK_000961"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6364250.1"
                     /translation="MPAFFAVLAATLIGGFFLFTKDDFFLLWKSALTALGFASNLYFA
                     RGKDYFAPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVARKSPRVQFGFLAALCALSL
                     AASFMPSAFDKYYLPHLRACELLIGSLTAVWMRCRQQRQPAIGRRCAAAGVLFAVCIL
                     SACLFAYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKFFQWKITVAAGLISYSL
                     YLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYHCIEKPFKKWKGSFAQSVL
                     WIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQPELL
                     VLGDSHADHYKTFFDAVGKKEKWAATMVSADACAYVEGYASRVFQNWAACRAVYRYAE
                     EHLPRYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHKLSSEKQAVYLMADNLA
                     SSYNVQRAYILSSRIPGYRQALRPDDESTLKANARIRELAAKYPNVYIIDAAAYIPAD
                     FQIGGLPVYSDTDHINPYGGTELAKRFSEKQRFLNTRHNH"
     gene            13254..14549
                     /gene="tyrS"
                     /locus_tag="ACFNMK_000962"
     CDS             13254..14549
                     /gene="tyrS"
                     /locus_tag="ACFNMK_000962"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC6364251.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGTKPEAQRILAEEMTRLIHGEAALQAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEISDGINAVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLNGEHKRFGKYTILRRGKRNHALLVWK"
     gene            15076..15996
                     /gene="ribF"
                     /locus_tag="ACFNMK_000963"
     CDS             15076..15996
                     /gene="ribF"
                     /locus_tag="ACFNMK_000963"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC6364252.1"
                     /translation="MKIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAALAGKTPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQGLNVRF
                     LHKLRDEEKFDGMAELKRQIAADMEAAKCW"
     gene            16137..18926
                     /gene="ileS"
                     /locus_tag="ACFNMK_000964"
     CDS             16137..18926
                     /gene="ileS"
                     /locus_tag="ACFNMK_000964"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC6364253.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAA
                     LAAAFGLASIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGITILAETSGDKLENLHMNHPFLECDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGRYIGETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PSINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGHETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         4450 a         5268 c         5351 g         4596 t
ORIGIN      
        1 gcgggcgcgg aagtaatcgg tttgttgccg ctgaaaatgg cgcgtgcaga ccatttggtc
       61 gggttgttgc cgcttaaatc aaagcggtac atattcccgc cgcgatcgcc ggcataggcg
      121 atatcgaccg tgccgtccaa atctttatcc accagcgtgg gggacgaaag cccgcccttg
      181 ccgtccttca cttcgatttt tgcaatcggc gtaccgttgt tgttttccaa atcatacaca
      241 tacagcgcgg ttgtattgtc gttgctgtta atgtctttag tcgcataacc ggaggcgagg
      301 aaagcggcgt atttgccgtt gtgggttttg ccgatttgcg gcgtgccgac ggtgtagcct
      361 aatttcacgc cattattgcc gttattattg tcatttttga catcaaacag ggaaacggcg
      421 gtcgggttat tgctgtcgac tttggtcaaa tccaaggcgt atgcgcctct accgccaaag
      481 cccatcgcgc cgaacataaa cacgtgtttt ttcccgcccc gttcgacttc gcgcaagaca
      541 aagccgccgt ccacgccgta gcggtcgccc acatagcctt tttcggcaaa ggtgcgcagc
      601 tcttgggcga gggtggagtc tttgagagcg gaagtgtcgt tatcaaaata ttggcgcggc
      661 atcgtgccag ggatgtagct gagcttcaga ctatagttgc gcgcgtcccc gttgcctttt
      721 ttgaagatat gcaccatccc gtcgttggcg gaagtagcca aatacccgcc gaccgccacg
      781 atggggctgt tgacgatgtc gcccaaatca cgctcgcgct tgctgttatc gcggctgcgg
      841 tatttttgac tgtatgttgg cttgccgttg ttttcttttt tgttgaatgt gttaaattga
      901 ccgtcatcat tggaagcacg aaccgtccaa ggcagcaata cttttttcca ctcgctggca
      961 tcaggcatga agctcccttc actaacaatg ccgaaagttg cgttgttgct gtttccatta
     1021 aaaccggtga cctcattgcc ttgaagtttg atctgatgta cgcctttatc caatcggatg
     1081 acggtttgcc gacttgtgaa agtcggctct ttactttgaa tatccgacgc actcaagtct
     1141 gcgagggaat agcggccggg tttgttgggg tcgttttttc ttttcagatt ttgaaggaag
     1201 atgcggctgc tcgaactgtc ggggtaggta gaaaccgaag cggaatacat ctgcacctta
     1261 ccattttgca cagggccgaa ccacagcgcg ggggcggtca gggccggcga agggggtttg
     1321 gctttggaat tggggtgggc ggcgttggtg cagcgcccgg ctttgacttc cggcaggtcg
     1381 aggcggactg taatgttccc tttatcttca atcttccacg tagcccaaaa atttagcaga
     1441 ttcccccccc gccttacctt tgcctcgacg cgcggtctca aggtgcccaa atccactgtt
     1501 ttgccttggg cgatatcttt aatccctttt ttgtccagca ggtgcagttt ggcgttcaga
     1561 taataggcca tggcggtatg ttcgcgctgc tggtcgccaa gggaaataag gtggcctcgt
     1621 gtcgtagaga cgatggtttg attattattg tcttcactaa aagaagatat caatctgttg
     1681 aatgagttgc catttttgac ggtattctcg ctgcccaaat tgaaagatac gcccaaccat
     1741 ggatgatctt tcaatttgta aatgggcgaa ttttcgcggc ttgggtccga gtgcctatcc
     1801 agcctgcccg cattttttac caaaccgatg ccgtcgaagg aaaatttggt ctcatagaca
     1861 agttgggggc attggtcgct gcttctgcat atgacgttgg tgtaggcgta gcctgccagc
     1921 ccggcccggg tggtatgaat ccaatcgacg gcattgttgc gctctttcag ctttctttca
     1981 tcaaaaccgg aaaccttgcc gtagggcggc aggtaggtgg ctgtgccaaa aacggcagta
     2041 ccgctttgtc gggaaacaag ggtatcggta ttgtcgaaaa agacagagcc tccaccctgc
     2101 ctgtctctat tatagataaa tttgcgttcc ctgtcttttt cccttaaggt tgaatatgaa
     2161 ccctcctgct ttaccttcag ctgattttgt tcgttcatga caatagcgta gttatgggtt
     2221 tccgcctgcg cgcccccccc gccggtatgg gaaaacatca agatggcggc ataaagcgcg
     2281 gtatggcgga aaacctgcct ttttaaagtt ttattcatcg tatttccttt tcggtcaaac
     2341 cccccgccct tcgggcatcc gtccttcggg gcggcaggat caggctttgt ccgggagggg
     2401 tgcaagccct tccgaatcag ggcggcgcct tatgtgccgc cctgtgtgtt ggaacatatt
     2461 ttatccctta aatcttgctt cgcgggatac cgtcggcggc aggcgcgccg caagcggcgg
     2521 gattcggcaa accgcccgat tccgcgcccg ccgaattgct ggttgcaaag cccccttcgc
     2581 ccggctgcca aagggggatg ttcgcaaagg taacgcgccc ctttggccgg atgcaatgta
     2641 tagggttacc gcattattct acccttgcgc gctgatcatg gtaaagcggt tttcggcaat
     2701 ccgcaaccgc ctccttggcc cgccattgcc gcgaaagcgg gaatccagac ctgtcggtgc
     2761 ggaaacttat cgggcaaaac ggtttcttga gattccgcat tctagattcc cgcctgcgcg
     2821 ggaatgacgg cgcataagtt tctgtgttga tttgagtcta tgggaacggt gtaggattgg
     2881 tggaatcgtt ggaatcggtg ggctgaagcc caccctataa ctcacctttt acgaatcctc
     2941 ctacacccta tacaacacct tgaaaatcca ccttgtcggg aatgcctaaa tcgtcagaaa
     3001 tacccgaacc gtcattcccg cgcaggcggg aatccagacc tgtcggtgca gaaacttatc
     3061 gggtataacg gtttcttgag attttacgtt ctagattccc actttcgtgg gaatgacggc
     3121 atcggtctgc ctttacaaca cggtttctta gattccacgt tctagattcc cgcctgcgcg
     3181 ggaatgacgg catatcggca ggcgcggggc ttgggacggc attgctgttg cggtttcgga
     3241 cgcggcttta ttcgacgaca attttaggga accggctgct gaagtctttg cctttgtcgg
     3301 caatgctcag ggcgatttgg aatgcggcat cggtgtagat tttggcgatg gcggggtgtt
     3361 cgtcgaacag ttgcgccggt gtgccgccgt ccattgcttc gcgcacgggc aggcttaggg
     3421 gaagctgtcc gagcaggggg acgttgaggc gggccgccaa atctttgccg ccgtccgtgc
     3481 cgaacagtgc ttcgctgtgt ccgcagttgg tgcagatgtg gacggacata ttttccaata
     3541 cgcccaaaat gggaatgttg actttgcgga acatatccac ggctttgcgc gcgtctatca
     3601 gggcgatgtc ctgcggcgtg gttacgatga ccgatccggt tacggggatg cgctgggaca
     3661 gcgtgagctg gatgtcgccc gtacccgggg gcaggtcgat gaaaagatag tccacttcgt
     3721 cccactcgct ttggaacatc agctgctgca aggcttggct gaccatcggc ccgcgccaga
     3781 cgacggcttg gccggtatcg acgagaaagc cgatagacat gacctgtatg ccgtctgaag
     3841 attcgacggg aatgagtttt tggtttttct gatcgggttt gcggtcgtcc acacccaaca
     3901 tggtcggttg gctcgggccg taaaggtcgg catcgagtac gccgacgcgc gcgcccatgc
     3961 gcgccattgc ggcggcaagg ttggctgtcg tcgttgattt gcccacgccg cctttgccag
     4021 aggcgacggc gatgatgttt ttcacgcctt tgatggtggt aacgccaggt tggactttgt
     4081 gtgtgccgat ttcagtgtcc atggacagat ggatgtgtgt gtcgcctgtt tcgggcatca
     4141 ggatttcctg tatgcggtcg gctgtttctg aggcaatgtg cgcgacgggg aagccgaaat
     4201 gcagggcgat atggatgccg tctgaacgct gttcgaccga acggacggcc ttttcgccgc
     4261 cgagcgtgcg tgccgtattc ggaacggcga cggtgtcgag gagggtgcgg atgttttgta
     4321 tgttcataat gtgtgtttct gtgtgcagtt tgggaaacgg gcggaaagtg tacattgatt
     4381 tcgggcggtt tgcctttcct tgtgcgaact tttacacggt atgcggcgga ttgtgaaaat
     4441 ccgttgtcaa aatatcccgt gcaaacagtg gtctgcaatt aagcctgtaa aatttgcttg
     4501 acgaggtagg atggtgctgt ataatgcacg gcttatcggg tcgttagctc agccggtaga
     4561 gcagcggact tttaatccgt tggtcgagcg ttcgaatcgc tcacgaccca ccagataacc
     4621 ggaagccaag ttttgagact tggctttttg tttgcctgcc ggttgcgcgg gcgtaccgtt
     4681 tgagagagcg tatgaaaaag aaactgcttt cgggcatcaa atttgccgtt cagacggcat
     4741 tggtgttttt attggtgtcg ctgtttttgg attggatacg caagcccgaa gagccggccg
     4801 gtgcggcagg gcggcctttg accctgctgt cggggcagcg gctgactttg ggacagttta
     4861 gccgagataa ggcggttttg gtgtattttt ggggaagctg gtgcggcgtg tgccgttatc
     4921 agtcgccgat aatcgatgat ttggcggcgg acggcgttcc ggtcgtcggc gtggcggtac
     4981 gttccggcag cgcgtccgaa gtggcggcgt atatggcaaa gcggggcttg ggctttccga
     5041 cggtcaacga tgaggacggg ggtttggcaa ggtcttggcg gattgtcgca acgcctgccg
     5101 tcgttttggt caaaaatggg aaaatggttc actatacgac gggaatcagc agttattggg
     5161 gcttgcgcgc acgaattttt caggcggatg ttttcggtta acttttgttt ttgttaaact
     5221 ttacgctatg tagagaatca aacggcatat accttgctcg tctgatgcgg cggcttttgt
     5281 tttttctgga agaaagttct ttcgacagta aatgtaatac tttgttgaga ttgtagggaa
     5341 aatagactaa gatataacta ttaaaatatt tttagatagg attatcggaa ttaaagtctt
     5401 ttatacccag tcgtccgatg cggtttatag cgtattgttg ctatatgttc gttttgttat
     5461 ataacggttg catcaaaaat tacgcccaca ggctttcccg acggtttgaa agtttgattt
     5521 tcgataactt ggagacttga acaatgccta cccaatcaaa acatgcgtct atcaatatca
     5581 gtctgataca ggcaagggaa gccctgatga cccaattcag gcctattctg aatcaggcga
     5641 atattaccga tcagcaatgg cggattatcc gtcttttggc ggaaaacggc acgctggact
     5701 ttcaagattt ggcgaatcag gcgtgtattc tgcgccccag cctgaccggt atcctgaccc
     5761 gccttgaaaa agcgggtttg gttgtccgcc tgaaaccttc caacgaccaa cgacgtgttt
     5821 ttctgaagct gactgccgag ggcgagaagc tgtatgagga aatcggcgaa gaagtggacg
     5881 agcgttacga cgctatcgag gaagtgctgg gccgcgagaa aatgctgctg cttaaagacc
     5941 tgttggcaga acttgccaaa atcgaggatg cgttgaactc gtaatacgcc gtaacgcgcg
     6001 gaaacgtccg accgacggct ttttgaatca gaactgctgc acatgggggg tgccttgtgt
     6061 gcagcattct tatatagggg acagtttaaa ggggaaaaat ggcggatttg cagaaaactt
     6121 ttcaaacttc gttccgtgat gcgatggcat cttgcgcggc aggcgttcat gtcatcacga
     6181 cagacggtgc ggcagggcgt tacggcatta caatgacggc ggttgcgccg gtaacggacg
     6241 agccgccgac cgtgatgctg tgcatcaacc gtaaagcccg tatcatcccg atactgtcgg
     6301 aaaacggcag cctgtgcatc aatacgctgg cggacgaaca tcaggatgtt gccgaacatt
     6361 ttgccgggct gaccggcctg tcgcccgaag agcggtttgc ctaccacatc tggcatcgcg
     6421 gcaaaacggg acaacttgaa atagaaggag cgttggcgca cctgcacggg catattgtcg
     6481 gcaaacatga aatcggcacg cattttgtgt tttacgtcag gctcgacgaa attaaaaact
     6541 gcgggtgcaa acgtcccgcg ctgctgtatt tcagacggca gtttagattt ttagactgat
     6601 attcggacag atatatgaaa gcgatgatac tggcggcagg acgcggcgag cgtatgcgcc
     6661 ctttgaccga taccactccg aagccgctgc ttgatgtggc gggtaagcct ctaatcggtt
     6721 ggcacctatg ccgtctgaag caggcggggt ttaccgaaat cgtcatcaac cacgcttggc
     6781 tgggtcggca gatagaagat gctttgggcg acggctcggc ttatggcgtg aacatcgcct
     6841 attcgcccga acccgcaggc ggtttggaaa cggcaggcgg catcgcgcag gcattgccgc
     6901 tgttgggtgg gcagccgttt ttggtggtca acggcgacgt gctgaccgac atcgatttta
     6961 ccgccgcgtt tcagacggca tcgtccctgc cggaacatat ttccgcccat ctgtggctgg
     7021 tggaaaatcc gccgcacaac cccgacggcg atttttccct gctgcccgac ggcagcgtgc
     7081 ggccggaagt aaatggcggc aacggattga catttagcgg cgtgggtatt taccgtcctg
     7141 aaatgtttga tggaatcgaa gcgggcagtg tggcgaaact cgcgcccgta ttgcggggcg
     7201 aaatgcggca aaaccgcgtc agcggacaga agcatacggg cttgtggctg gatgtcggca
     7261 cggtatgccg tctgaaagag gctcaagccc ttgcaggggc ttggaagtga aataaaacgg
     7321 tggcttgccg agtcggctgc cgaacggttc cgaccggatg tccggcatat tttcgtggag
     7381 gcgtgaaaat agggcgttcg gggctttccg tccaacaaat gccatctgaa gccgaccctg
     7441 tcggatgcgg cggtttgccg tcggcgcggt gtcggatttt attgggtaac tttgttcaga
     7501 ccgccgcccg tcagattgtc aaaacccggt ttcaggcggt atggcggatt cggtttaacg
     7561 tttcaacgcc agcaccaaca cgcccgccgt taccagcccc aagcctatcc attcctgcgt
     7621 gttcgggcgt tcgtccaaga aaaccaccgc catcagcgcg accagaacca ggctgaattt
     7681 atcaacgggg gcgacttgcg aggcgttacc cagttgcagg gctttaaaat aggcgagcca
     7741 ggatgcgccg gtggcgagtc cggataaaat cagaaacgtc cagttgcggt cggtaaagcc
     7801 gtttacgccc tgccatttgc cggtgtaggt taaaaacaat accaaagcgg cgaggatgac
     7861 caaggtgcgg atgaaagtgg caaaatcgga atctattcct tgtaaaccca ttttggcgaa
     7921 aacggcggtc aatgaggcga agcctgccga tgccaatgcc caaaacagcc atgcgttgct
     7981 gcccatgttt tctccttgga ttgtgaacaa tatgaacggt atttttgttg cagcgtcaaa
     8041 aatttcactg cgggtttggt gcggataacg ttataatatg cctgatatta ttttcaatcc
     8101 acctgtttgt cgcctgatgc tttcagacgg catgtccctc ctcatttcta aaggaaaatc
     8161 atgagcttca aaaccgatgc cgaaatcgcc caatcctcca ccatgcgccc gattggcgaa
     8221 attgccgcca agctgggcct gaacgttgac aacattgagc cttacggtca ttacaaggcg
     8281 aaaatcaatc ctgccgaagc gttcaaactg ccgcaaaaac agggcaggct gattttggtt
     8341 accgccatca acccgactcc ggcgggcgaa ggcaaaacca ccgtaaccat cggtttggcg
     8401 gacgcgttgc gccacatcgg caaagatgcc gtgattgccc tgcgcgaacc ttctctgggg
     8461 ccggtgttcg gcgtgaaagg cggcgcggca ggcggcggct atgcccaagt tttgccgatg
     8521 gaagacatca acctgcactt caccggagat tttcacgcca tcggtgcggc aaataatctg
     8581 cttgccgcga tgctcgacaa ccatatctat cagggcaacg agttggacat cgaccccaaa
     8641 cgcgtgctgt ggcggcgcgt ggtcgatatg aacgaccgcc agttgcgcaa catcatcgac
     8701 ggcatgggta aacccgttga cggtgtgatg cgccccgacg gttttgatat caccgttgcc
     8761 tccgaagtga tggcggtatt ctgccttgcc aaagacatca gcgatttgaa agagcgtttg
     8821 ggcaacatcc ttgttgccta cgccaaagac ggcagccccg tttacgccaa agatttgaaa
     8881 gcacacggcg cgatggcggc attgcttaaa gatgcgatta agcccaactt ggtgcaaacc
     8941 atcgaaggca cacctgcctt cgtacacggc ggcccgttcg ccaacatcgc ccacggctgt
     9001 aactctgtaa cggctacccg actggcgaaa caccttgccg attacgccgt aaccgaagca
     9061 ggcttcggcg cggatttggg cgcggaaaaa ttctgtgaca tcaaatgccg tctggcggat
     9121 ttgaaacctg atgcggctgt tgtcgtcgct accgtccgcg ctttgaaata taacggcggc
     9181 gtggaacgtg ccaacctagg cgaagaaaac ctcgaagccc tggcaaaagg tttgcccaac
     9241 ctgctgaaac acatttccaa cctgaaaaac gtattcggac tgcccgtcgt cgttgcgctc
     9301 aaccgcttcg tgtccgactc cgatgccgag ttggcgatga ttgaaaaagc ctgtgccgaa
     9361 cacggcgttg aagtttcctt gaccgaagtg tggggcaaag gcggcgtggg cggtgcagac
     9421 ttggcgcgca aagtcgtcaa cgccatcgaa aaccaaccga acaacttcaa tttttcctac
     9481 gatgttgagc tgagtattaa agacaaaatc cgtgcgattg cccaaaaagt gtacggcgcg
     9541 gaagatgttg atttcagcgc ggaagcgtct gccgaaatcg cctcgctgga aaaactgggt
     9601 ttggacaaaa tgccggtctg tatggcgaaa acccaatatt ctttgagcga caacgccaaa
     9661 ctgttgggct gccccgaagg cttccgcatc gccgtgcgcg gcatcaccgt ttccgcaggc
     9721 gcaggtttca tcgtcgccct gtgcggcaac atgatgaaaa tgcccggcct gcccaaagtt
     9781 ccggctgccg agaaaatcga tgtggacgca gaaggcgtga ttcacggctt gttctgaacg
     9841 gttttctgaa accggatgcc gtctgaagcc gtttcagacg gcattttttc ggaacgcggg
     9901 cggcggtatg ctataatccg ccgttaaatt tctctatttt caggaaaaaa catgagtttg
     9961 aaatgcggca tcgtcggttt gcccaacgtc ggcaaatcca ccctttttaa cgcgctgacc
    10021 caatcgggta tcgaagcggc aaactatcct ttctgtacca tcgaacccaa cgtcggcatc
    10081 gtcgaagtcc ccgatccgcg tatggccgaa ttggcaaaaa tcgtcaatcc gcaaaaaatg
    10141 cagcctgcca tcgtcgaatt tgtcgatatt gccggtttgg ttgcaggcgc gagcaaaggc
    10201 gagggcttgg gcaaccagtt ccttgccaac atccgcgaaa ccgatgcgat tgtgaatgtc
    10261 gtgcgctgct ttgacgacga caacatcgtc cacgttgcag gccgcgtcga tccgattgcc
    10321 gacattgaaa ccatcggtac agagttggcc cttgccgact tggcaagtgt ggaaaaagcc
    10381 atcgtccgcg aagaaaaacg cgcccgttcc ggcgacaaag acgcgcaaaa actggtcgaa
    10441 ctgtgtaaaa aactgctgcc gcatctggac gaaggcaaac ccgtgcgttc cttcggtttg
    10501 gacgcggaag aacgcgcgat gctcaaacca ctgttcctgc tgaccgccaa accggcgatg
    10561 tatgtgggca acgtcgccga agacggtttt gaaaacaatc cgcacctcga acgcctgaaa
    10621 gaattggcgg caaaagaaaa cgcccccgtc gtcgccgttt gcgccgcaat ggaaagcgaa
    10681 attgccgaat tggaagacga cgaaaaagcc gaattcctcg ccgaaatggg cttggaagaa
    10741 cccggcctga accgcctgat tcgtgccggt tacgacctct tggggctgca aacctatttc
    10801 accgccggcg tgaaagaagt ccgcgcgtgg acgatacaca aaggcgacac cgccccgcaa
    10861 gccgccggcg tgattcacac cgactttgaa cgaggcttca tccgcgccca agtcatttct
    10921 tacgacgact ttgtctcgct cggcggcgaa gccaaagcca aagaagccgg caaaatgcgt
    10981 gtggaaggca aagaatacgt cgtgcaagac ggcgatgtaa tgcacttttt gtttaacgtg
    11041 taacccaaat gcggcaggtt ttcagacggc tttgccggaa atgccgtctg aagccggttt
    11101 tggtggtttt cggcgtttcc ataccgccgg aatgcggccg catcaaaata aaatcccgcc
    11161 cgcatttccg atttgccctc cccgattcct gcaaaacaaa ccgcctgccc cgctgttgca
    11221 aaaatccgtc cggcattccg aatatcccga gccccgatac aaaatgacct ttcagatggc
    11281 atttgcgccg ccgcgatgaa agcaaaaata ttatgagcca agccttaccc taccgcccgg
    11341 acatcgacac attgcgcgcc gccgccgtct tgtccgtcat cgtgttccat atcgaaaaaa
    11401 attggctgcc gggcggattt ctcggtgtcg atatattctt tgtgatttca ggctttttga
    11461 tgacggcgat cctccttcgc gaaatgtcgg gggggggggg aggttttccc tgaaggcatt
    11521 ttatatccgc cgcatcaaac ggattttgcc cgcatttttc gccgtattgg cggcaacgct
    11581 gataggcggc ttctttttat tcaccaaaga tgatttcttt cttttatgga aatccgcgct
    11641 gaccgccttg ggtttcgcat ccaacctgta ttttgcaagg ggcaaggact atttcgctcc
    11701 cgcgcaggaa gaaaagcccc tgctgcacat ttggtctttg tcggtcgaag aacaatttta
    11761 ctttgtcttt ccgatattgc tgctgcttgt cgcccgcaaa agcccgcgcg tacagttcgg
    11821 cttcctcgcc gcactgtgcg ccttaagcct tgccgcttcc tttatgcctt ccgcgttcga
    11881 taaatattac ctgccccacc tgcgcgcctg cgaattactg ataggatcgc tgaccgccgt
    11941 gtggatgcgc tgccggcaac agcggcaacc cgctatcggc agacgctgtg ccgccgccgg
    12001 cgtattgttt gccgtgtgca tactgtccgc ctgcctgttt gcctattcgg aacaaaccgc
    12061 ctatttcccg ggccccgccg ctttgattcc ctgtctggct gttgccgcgc tgatttattt
    12121 caaccattac gaacacccgc ttaaaaaatt tttccaatgg aaaatcaccg ttgccgccgg
    12181 tttgatttcc tattcgcttt atctgtggca ttggccgata ttggccttta tgcgctatat
    12241 cggcccggac aacctgccgc cttattcgcc ggcggcagcg atcgtcctga ctctagcgtt
    12301 ttccctgatt tcttatcact gcatcgaaaa gccgtttaaa aaatggaaag gttcgttcgc
    12361 acaatccgtt ttatggattt atgccttgcc tatgctcatt ttgggggcgg gctcgttttt
    12421 cgcgatgagg ctgccgttta tggcgcaata cgaccgcttg gggctgacgc gttccaacac
    12481 ctcctgccac aacaataccg gcaaacaatg cctatggggg gatacggaaa aacagccgga
    12541 actgctggtt ttgggcgact cccacgccga ccattacaaa acattcttcg atgccgtggg
    12601 caaaaaagaa aaatgggctg ccactatggt ttccgccgac gcttgcgcct atgtggaagg
    12661 ctacgcgtcc cgcgtattcc aaaactgggc cgcctgccgc gccgtttacc gctatgccga
    12721 agaacacctg ccccggtatt caaaagtggt tttggcgatg cgctggggca gccaaatgcc
    12781 cgaaaacagc cgctcccttg cctatgatgc cggttttttc caaaaattcg accgtatgct
    12841 gcacaaactc tcatccgaaa aacaagccgt ttacctgatg gcggacaact tggcctcgtc
    12901 ttacaacgtc cagcgcgcct atatcttgtc ttcccgcata ccgggttacc gccaagccct
    12961 gcgcccggac gacgaaagca ccctgaaagc caatgcacgc atcagggaat tggcagccaa
    13021 ataccccaac gtctatatta ttgatgccgc cgcctatatc cccgcagatt ttcaaatcgg
    13081 cggattgccg gtttactcgg acacagacca catcaaccct tacggcggca cggaattggc
    13141 aaagcgtttt tccgaaaaac aacggtttct caatacgcgc cataaccatt gattcgctta
    13201 aatttgttac aatcggcggt ttgcaaaaac gctaattttt ttgaaagaga ccgatgagcg
    13261 tcatccaaga cctgcaatcg cgcggcctaa tcgcgcaaac gaccgacatc gaagccttag
    13321 acgctttgtt gaacgaacaa aaaatcgccc tttactgcgg tttcgatccg accgccgaca
    13381 gcctgcacat cggacacctg ttgcccgtat tggcattgcg ccgcttccaa caggcggggc
    13441 atacgccgat tgctttggtg ggcggcgcga ccggtatgat cggcgacccc agcttcaaag
    13501 ccgccgaacg cagcttgaat tccgccgaaa ctgttgccgg ctgggtggaa agtatccgca
    13561 accaattaac ccctttcttg agctttgaag gcggaaatgc cgccattatg gcgaacaatg
    13621 ccgactggtt cggcagcatg aactgccttg acttcctgcg cgacatcggc aagcatttct
    13681 ccgtcaacgc catgctgaac aaagaatccg tcaaacagcg catcgaccgc gacggcgcag
    13741 gcatttcctt caccgagttc gcctactccc tgctgcaagg ctacgacttt gccgagttga
    13801 acaaacgcca cggcgcggtt ttggaaatcg gcggctccga ccaatggggc aacatcaccg
    13861 ccggtatcga cctgacccgc cgcctgaatc aaaaacaagt gttcggcctg accctgcctt
    13921 tggttaccaa atcagacggc accaaattcg gcaaaaccga aggcggcgcg gtgtggctga
    13981 acgcaaaaaa aacctcgccg taccagttct accaattctg gctgaaagtc gccgatgccg
    14041 atgtgtataa attcctgaaa tactttacct tcctgtccat cgaagaaatc gatgccatcg
    14101 aagccaaaga caaggcaagc ggcaccaagc ccgaagcgca acgcatcctc gccgaagaaa
    14161 tgacccgcct gattcacggc gaagccgccc tgcaagccgc gcagcgcatt tccgaaagcc
    14221 tgtttgccga agaccaaagc agcctgaccg aaagcgactt cgagcagctc gccctcgacg
    14281 gcctgcccgc atttgaaatt tcagacggca tcaacgccgt cgaagccttg gtaaaaaccg
    14341 gtttggcatc ctccaataaa gaagcgcgcg gctttgtgaa cagcaaagcg gttttgctca
    14401 acggcaaacc tgccgaagcc aacaacccca accacgccgc cgaacgcccc gacgatgcct
    14461 gcctgctgaa cggcgaacac aaacgtttcg gcaaatacac catccttcgg cgcggcaaac
    14521 gcaaccacgc gcttttggtt tggaaataat ccgattgccg cagaaatgcc gtcattcccg
    14581 cgcaggcggg aatccggacc tgtccgcacg gaaacttatc gggcaaaacg gtttcttaga
    14641 ttccacgttc tagattcccg cctgagcggg aatgacgagt ttcaagatta cggtgttgtc
    14701 ggaacgcaac tgaaccgtca ttcccacgaa agtgggaatc tagaatctcg gggtttgagc
    14761 aactgttttt atccgataag tttctgtgcg gacaggtccg gattcccgcc tgcgcgggaa
    14821 tgacggcgga gggttgtttg tctcggttta cctggttaaa aaagaacgat tttcactgat
    14881 gttgcatcag gtttggggcg atgtttcaac acatagcacc gcgcctgctg cgcgctttat
    14941 gcgtttggcg cgttcggctg cgggaaattt gcctactttt cccgcgtcgg gcgggcgtaa
    15001 cgggcggcac actgtctata aaccgcaata ccgtttacaa tgaccgcctg tttcaccaca
    15061 tacccgaacg caacaatgaa aatcaggctg gggcggcaca acgcgcccga ctttccgcac
    15121 ggggccgccg taaccatagg caatttcgac ggcgtacacc ttggacacaa acacatcctc
    15181 caaaaactcc gcctcgaagc cgacgcgcgc ggactgcccg tcgttgccgt catcttcgag
    15241 ccgcaaccca aagaattttt tgccgccctc gcaggcaaaa ccccgccctg ccgcatcagc
    15301 cccctgcgta ccaaactgga attgctggaa gggacgggct gcgtcgatgc cgtctgggtt
    15361 ttgcgtttcg atcaaaattt ttccgaaata tccgcgcaag ggtttatcga ccgcctgctg
    15421 cgtcaaacct tgaatacgcg ttatttgctc gtcggcgatg atttccgttt cggtgcgggg
    15481 cgggaaggct gttttgaact tttggcacaa cagcccgata tgcagaccga gcgtacgcct
    15541 tccgtcatcg tcgaagacat ccgcaccagc agtaccgccg tgcgacaagc cctttcagac
    15601 ggcaaccttg cctatgcgaa aaaacttttg ggacacgact acgtcttgag cggcagggtg
    15661 gtgcacggca gaaaactcgg acgcacctta aacgccccga ctgccaacat ccgcctgccc
    15721 cgccaccgtt atgcactcgg cggcgtgttt gtcgtcgaag cagacggcgc attcggcacg
    15781 cggcgcggcg tggcgagttt cggcttcaat cccaccgttg atagcggctg ttctcaaaag
    15841 cttgaagtcc acctgttcga ctttcaaggc gacctgtacg gacaagggct gaacgtccgc
    15901 ttcctgcaca aactgcgcga tgaagaaaag tttgacggta tggcagaact gaaaaggcag
    15961 atagcagccg atatggaagc cgcaaagtgt tggtagaaaa accttataca aaccatccga
    16021 ttgggctaca atcaatcttt taacttttca gacggcatag gggtttcccg ttgtgaaata
    16081 ctgtttgagg cgcaatgccg tctgaaccga aatattgtaa caatagagat taaaaaatga
    16141 ccgattacag caaaaccgta aacctgctcg agagcccgtt tccgatgcgc ggcaatcttg
    16201 ccaagcgcga gcctgcatgg ctgaaaagct ggtacgagca aaaacgctac caaaaactgc
    16261 gcgaaatcgc caaaggccgt ccgaaattca tcctgcacga cggcccgccg tatgccaacg
    16321 gcgacatcca catcggtcat gccgtcaata aaattctcaa agacatcatt atccgcagca
    16381 aaacccaagc cggttttgac gcgccttatg tgccgggttg ggactgccac ggcctgccca
    16441 tcgaagtgat ggtagaaaaa ctgcacggca aagatatgcc caaagcacgt ttccgcgaat
    16501 tgtgccgcga atacgccgcc gaacagattg cccgtcagaa aaaagacttt atccgcttgg
    16561 gcgtgttggg cgactgggac catccttacc tgactatgga tttcaaaacc gaagccgata
    16621 ccgtgcgtat gctcggcgaa atctacaaat ccggctatct ctaccggggt gcgaaaccgg
    16681 ttcaattctg cttggactgc ggttcttcgc tggccgaagc ggaagtggaa tataaagaca
    16741 aaatctcgcc cgcgattgac gttgcctatc cgtttaaaga cactgccgcg cttgccgccg
    16801 cattcggttt ggcaagtatc gaaggcaaag cgtttgccgt tatttggaca accacgcctt
    16861 ggactttgcc tgcgagccaa gctgtttcag cgggtgcaga cgtggtgtat caattaatcg
    16921 atacgcccaa aggcaaattg gtattggcca aagatttggc ggaagacgct ttgaaacgct
    16981 acggcttttc agacggcatc accatccttg ccgaaactag cggcgacaag ctggaaaacc
    17041 tgcacatgaa ccatccgttc ctcgaatgcg atattcccat gctcaacggc gaacacgtta
    17101 ccaccgatgc cggtaccggc ttggtacaca ccgcccccgc gcacggtttg gaagactacg
    17161 ccgtctgcaa taaatacggc atcgagcttt acaaccccgt caacgccgaa ggccgataca
    17221 tcggcgaaac gccgcgcgtc gccggtatgc gcgtttggga ggcgaacccc gtcatcctgc
    17281 aatggttgga agaaaccggc aaccttttgg caagcagtaa aatcgaacac agctacgccc
    17341 actgctggcg gcacaaaacg ccgctgattt accgcgcgac aggtcaatgg tttgtcggta
    17401 tggacaaagc cggtgccgac ggcaaaaccc tgcgcgacaa agccatcaag gccgtggacg
    17461 acaccgaatt cttcccgtct tggggtcgcg cgcgtttgga agccatgatc gaaggccgcc
    17521 ccgactgggt cgtttcgcgc caacgctact ggggcacgcc gatgaccttc ttcgtccaca
    17581 aagaaaccgg cgagctgcat ccgaactctg ccgaactttt ggaaaaagtc gcgcaacgca
    17641 ttgaagaaaa aggcatcgag gcttggttct ccctcgataa aagcgaatta ttaagcgcgg
    17701 aagattgcga acattacgac aaactccccg atacgatgga cgtatggttt gactccggct
    17761 cgacccatta ttccgttgtg aaacaacgcg aagaattgga atggccggct gatttgtatc
    17821 tcgaaggcag cgaccaacac cgcggctggt tccaatcgtc tatgttgacc ggttgcgcct
    17881 catccatggg tcgtgcgcca tacaaacagc tgctaaccca cggtttcgtg gttgaccaaa
    17941 acggtcgcaa aatgtcgaaa tccatcggca acgtcgtcgc gccgcaagaa gtctataacg
    18001 aattcggcgc ggacatcctg cgcctgtggg cggcatccac cgattacagc ggcgaattgg
    18061 cgatttccaa agaaatcctc aagcgcgtaa ccgaaagcta ccgccgtatc cgcaatacct
    18121 tgagcttcct gtttgccaat ctcagcgact tcaatcctat tgaagatgcc gtgcaacagg
    18181 cagacatggt ggaaatcgac cgctacgccc tagtgttggc gcgtcggctg caagagcgtc
    18241 tggcaggcga ttactatccg cgttatgcct tccacttcgc cgtaaaagac attgtttctt
    18301 tctgctcgga agacttgggc gcgttctacc tcgatatcct gaaagaccgc ctctacacca
    18361 ccaaagcaga cagccatgca cgccgcagcg cacaaactgc cctgtatcac atcacacgca
    18421 gtttggttct gctgattgct ccgattttgt gcttcaccgg cgaggaagca tgggacatca
    18481 tcggcggagg agaagaagac agcgtcctct tccacacatg gcatgagttc ccatctatca
    18541 atgaaaaagc cgaagccgaa ttggtgaaaa aatggacggc aatccgcgaa gcccgcgaag
    18601 cggtaaccgc cgccatcgag cctttgcgcg ccgacaaaac cgtcggttcg tccttgcaag
    18661 ccgaagccga aattaccgcg ccggaagaaa tggccggcta tctgaatgct ttgggcgaag
    18721 aattgcgctt tgctttgctg gtgtctaaag cagaagtgaa agtaggcagc gaacttgccg
    18781 ttgccgctaa agccagtgat ggtgaaaaat gcgaacgctg ctggcactac acccgcgatg
    18841 tgggcgcggt tgcaggccat gaaaccgtct gcaaacgctg tgcagagaat gtcggcggag
    18901 aaggcgaaac gcgccattac gcctgataaa gtttgagcaa atgccgtctg aaaccgccga
    18961 acagcatttc agacggcatt ttttgtgccg cgatttgtct ttataacggc ggaggggttt
    19021 caagattgcg gtgttgtcgg aatgcaactg aaccgtcatt cccacgaaag tgggaatcta
    19081 gaatctcgag gtttcagtca tttccgatag attcccgcct gtgcgggaat gacggatttc
    19141 gagattacgg tgttgtcgga acgcaactga accgtcattc ccacgaaagt gggaatctag
    19201 aatctcgggg tttcagtcat ttccgataga ttcccgccgc gtcggaggtc tggattcccg
    19261 cctgcgcggg aatgacgggt ttcaagattg cgctgttatc gggaatgacg gatttcaaga
    19321 ttacggtgtt gtcggaatgc aactgaaccg ccattcccac gaaagtggga atctagaatc
    19381 tcggggtttc agtcatttcc gatagattcc cgccgcgtca ggggtctgga ttcccgcctg
    19441 tgcgggaatg acggatttcg agattgcggt gttgtcggaa cgcaactgaa ccgtcattcc
    19501 cacgaaagtg ggaatctaga atctcggggt ttcagtcatt tccgatagat tcccgccgcg
    19561 tcaggggtct ggattcccgc ctgtgtggga atgacggatt tcgagattgc ggtgttgttg
    19621 gaacgcaact gaaccgtcat tcccacgaaa gtgggaatct atagt
//
LOCUS       DAXHTY010000033        19486 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXHTY010000033 DAXHTY010000000
VERSION     DAXHTY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19486
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.033"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(92..1339)
                     /gene="fabF"
                     /locus_tag="ACFNMK_000965"
     CDS             complement(92..1339)
                     /gene="fabF"
                     /locus_tag="ACFNMK_000965"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC6364254.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGVLVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(1495..1731)
                     /gene="acpP"
                     /locus_tag="ACFNMK_000966"
     CDS             complement(1495..1731)
                     /gene="acpP"
                     /locus_tag="ACFNMK_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC6364255.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            1963..2970
                     /locus_tag="ACFNMK_000967"
     CDS             1963..2970
                     /locus_tag="ACFNMK_000967"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC6364256.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNMVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGGDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            2986..3165
                     /locus_tag="ACFNMK_000968"
     CDS             2986..3165
                     /locus_tag="ACFNMK_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364257.1"
                     /translation="MPSERTFCRSDGIFISFFRLTPLENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            3303..3626
                     /locus_tag="ACFNMK_000969"
     CDS             3303..3626
                     /locus_tag="ACFNMK_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364258.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFACPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            3713..3994
                     /locus_tag="ACFNMK_000970"
     CDS             3713..3994
                     /locus_tag="ACFNMK_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364259.1"
                     /translation="MENGQRNNRFSLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            4047..4520
                     /locus_tag="ACFNMK_000971"
     CDS             4047..4520
                     /locus_tag="ACFNMK_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC6364260.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            4534..4860
                     /locus_tag="ACFNMK_000972"
     CDS             4534..4860
                     /locus_tag="ACFNMK_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364261.1"
                     /translation="MPSEALSGLQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WNGVAKAVGEGMIEAANEHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKGAAYAVL
                     KERGEV"
     gene            5058..7748
                     /gene="glnE"
                     /locus_tag="ACFNMK_000973"
     CDS             5058..7748
                     /gene="glnE"
                     /locus_tag="ACFNMK_000973"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC6364262.1"
                     /translation="MSDNRLDAARRHSLFLARNLDSGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDAIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRDTKSPQHLSVVAMGKAGGYELNVSSDIDLIFTYPESG
                     DTDGRHERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGNSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAELGIMP
                     SENVETLLAAYRFLRDIEHRLQYWDDQQTQTLPTSPEQQQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAEQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAEIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALSGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLANYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(8048..8740)
                     /locus_tag="ACFNMK_000974"
     CDS             complement(8048..8740)
                     /locus_tag="ACFNMK_000974"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6364263.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSRPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(8848..10041)
                     /locus_tag="ACFNMK_000975"
     CDS             complement(8848..10041)
                     /locus_tag="ACFNMK_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6364264.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(10170..10907)
                     /gene="pxpA"
                     /locus_tag="ACFNMK_000976"
     CDS             complement(10170..10907)
                     /gene="pxpA"
                     /locus_tag="ACFNMK_000976"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFC6364265.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(10894..11823)
                     /locus_tag="ACFNMK_000977"
     CDS             complement(10894..11823)
                     /locus_tag="ACFNMK_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFC6364266.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(11820..12467)
                     /gene="pxpB"
                     /locus_tag="ACFNMK_000978"
     CDS             complement(11820..12467)
                     /gene="pxpB"
                     /locus_tag="ACFNMK_000978"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFC6364267.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            12651..14858
                     /gene="uvrD"
                     /locus_tag="ACFNMK_000979"
     CDS             12651..14858
                     /gene="uvrD"
                     /locus_tag="ACFNMK_000979"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC6364268.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTRRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQLSLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNATLESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKPAYDSYGNTRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            15215..15754
                     /locus_tag="ACFNMK_000980"
     CDS             15215..15754
                     /locus_tag="ACFNMK_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6364269.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(15944..16159)
                     /locus_tag="ACFNMK_000981"
     CDS             complement(15944..16159)
                     /locus_tag="ACFNMK_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364270.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(16283..16642)
                     /locus_tag="ACFNMK_000982"
     CDS             complement(16283..16642)
                     /locus_tag="ACFNMK_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6364271.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            16695..17072
                     /locus_tag="ACFNMK_000983"
     CDS             16695..17072
                     /locus_tag="ACFNMK_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6364272.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVQISILKQLVKSSLS"
     gene            17271..17891
                     /locus_tag="ACFNMK_000984"
     CDS             17271..17891
                     /locus_tag="ACFNMK_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6364273.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPESIIPLFCIAEASIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            17914..19365
                     /locus_tag="ACFNMK_000985"
     CDS             17914..19365
                     /locus_tag="ACFNMK_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6364274.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
BASE COUNT         4760 a         5124 c         4942 g         4660 t
ORIGIN      
        1 ccgccttgtc ctgatttttg ttaatcacta taaaaatgcc gtctgaaacg gtttcagacg
       61 gcatttcgat gtcggcggcg gctttgcgga atcagccttt gaagcgtttg aagaccagcg
      121 tgccgttggt gccgccgaag ccgaaggagt tggaaatggc aacgtcgatt tccgcgtcgc
      181 gcgcttcgtt ggcgcagtag tccaaatcgc agccggcttc aacgtcttgt tcaaaaatgt
      241 tgatggtcgg cgggattttg ccgtcgtgta tcgccaaaat gctgtacacg gcctccacgc
      301 cgcccgccgc gccgagcagg tggccggtca tggatttggt cgagctgacg acggttttgt
      361 aggcgtgttc gccgaacgcg cgtttgaggg ctttggtttc gttggcatcg cccaaggggg
      421 tggacgtgcc gtgcgcgttg acgtaatcca cgtcttcggg attgatgccg gcatctttca
      481 gcgcgcgggt aacggcaagg gcggggcctt cttcgttcgg cgcggtgata tggtaagcat
      541 cggaactcat gccgaagccg acgatttcgg cgtaaatttt cgcaccgcgt tttttggcgt
      601 gttccaattc ttccaacacc aatacgcccg cgccttcgcc gataacgaag ccgtcgcggc
      661 ctttgtccca cggacgggaa gcggtggcgg gatcgtcgtt gcgggtggag agggctttca
      721 tcgcggcaaa accgcccacg cccaaagtgc tgattgcgcc ttccgcgccg ccggcaacca
      781 ttatgtccgc gtcgccgtat ttgatcagtc gggcggaatt gccgatggcg tgcgcgccgg
      841 tggtacaggc ggaaaccatc ccgtagctcg ggccgcggta gcctttgagg atggtaacgt
      901 gtccggaaat caggttgatc agcgaaccgg ggataaagaa agggttgatt ttgcgcgcgc
      961 cgccttcgat tacggctttg ccggtgacct cgatgctggg cagtccgccg atgccggaac
     1021 cgatgttcac gccgatgcgg tctttgtcga ggttttccac atcgtccaag cccgaatcgg
     1081 cgattgcctg caatgcggcg gcaatgccgt agtggatgaa tacgtccatc cggcgcgctt
     1141 ctttcgcgct gatgtattgt ccgatgtcga aaccgcgcac ctcgccggcg acacggctgt
     1201 tgatgtcgga tgcgtcaaag cgggtaatcg cgccgatgcc gcttttgccg gcgagcaggg
     1261 tgtcccaagc ctctgcgaca gtgttgccga caggggaaac ctgacctaag cctgtaatga
     1321 ctactcttct ctgactcatg ataacctcgc tgttggttgt cggaatgggg gcatatgcgg
     1381 ctgtcgtgca gatgccgtct gtaatttgcg gcaggggttc aaacagtttg ccatataagg
     1441 gaaaagcctc tattgcgcgg tgcagcagag gctgttgtgt cgggcgacga ccggttagcc
     1501 gttgtgggca ttgatgtagt cgatagccag ttggacggtg gtgatttttt cggcatcttc
     1561 gtcggggatt tcgcagccga atgcttcttc caaagccata accagctcca cggtgtccaa
     1621 agaatccgcg cccaagtcgt cttggaaaga agattcgttt ttcacgtcgg cttcgtttac
     1681 gcccagttgt tcagcaacaa tttttttaac ttgttgttcg atgtttgaca tatcagtcgt
     1741 tcctttatgc cttgcggcag gttgtttaag ggaaataaat cggtggtatt gtaccgactt
     1801 ttaatagagt tttctatcta atgactatta tatcaatatt tgccgatttg tacatttttg
     1861 ggtgcggcgg gttttgtcgt tcaagtttga cctgtgtgcc gtatgtttga cgggatttcg
     1921 gttaaaatga cggcatttcc atctgaagca gaaagccctg tcatgtatcc acttgcccgc
     1981 cgcatcctgt ttgcactcga tgccgagaaa gcccaccact tcacgctcga cgcgctcaac
     2041 atggtttata aattgggtct gattcccgta accggcaacc gtaccaaacc tgtaaaactg
     2101 atgggtttgg atttgcccaa ccctgtcggg cttgccgccg gactcgacaa aaacggcgaa
     2161 tatatagacg cattgggcgc gctcggcttc ggttttatcg aaatcggcac ggtaacgccc
     2221 aacccgcagc ccggcaaccc gcagccgcgc ctcttccgcg ttcccgaata tcaaggcatc
     2281 atcaaccgca tgggcttcaa caaccacggc atcgatgcca tgattcaaaa catcgaaaaa
     2341 agtaaattca gcggcgtatt gggcatcaac atcggcaaaa acgctgtaac accgattgaa
     2401 aacgctgccg atgattattt aatctgcctc gaaaaagcct acgcacacgc aagttacatt
     2461 accgtcaata tttcctcgcc caacactaaa aacctccgcg cgctgcaagg tggcgacgag
     2521 ttgagcgcat tgcttgaggc tttgaaaaac aaacaggcac agcttgcctc tgtacacggg
     2581 aaatacgtcc cgctcgccgt caaaatcgcc cccgatttgg atgaagcaca aatcgaagac
     2641 atcgcccacg ttgtcaaatc cgtcgaaatg gacggcatca ttgctaccaa caccaccatc
     2701 gacaaatcaa gtctcggcag ccatccgctc gcaggcgagc agggcggttt gagcgggctg
     2761 cccgttcatg aaaaaagtaa tcgggtgttg aagctgttgg cagaccacat cgacggcaag
     2821 ctgccgatta tcggcgtagg cggcattatg gaaggcgggg acgcggcaga gaaaatccgc
     2881 ttgggcgcga ccgccgtcca ggtgtacagc ggattgatat acaaaggtcc ggcattggtc
     2941 aaagaatgtt tgaaggcttt ggcgcgatga cgcgatccgc ccaaaatgcc gtctgaacgc
     3001 acgttttgcc gttcagacgg cattttcatt tcctttttcc gcctgacgcc ccttgaaaat
     3061 cccttacgcg ccgccctgtt tgaaacaaag caaacgcccg ccgcgaaccg gacaatcttg
     3121 atacgcagcc aaagcaagtc cggcggtaag ttgttaaata tataagcaat tgatacaaaa
     3181 aataatttta tactgtggcg gacggcttgg gaaaacgcaa aatatgaacg gcgttgaaat
     3241 tcatgatgtg gaaaataagg atacggcgga taatcaaaag attttatatt taaggatttg
     3301 atatgtttaa ggacgaacta aatgaattta tccgattgat atccgatccg gaaagtgaat
     3361 tggatgaatg gtatttgagt gattttaaag atgaacatat atgggaaatg caaagttacg
     3421 aagcattttc atgtttgcgg gaagcagtcc cctatttgtt tgcttgtccc cgatacgggt
     3481 atgaactgtt ggagataatt tccgcgttaa aagaaacatc tgatacgaca gaattatttt
     3541 atgagccggg tatcgtgcct ttgctgattg atttatataa agaagatagt tatctggtaa
     3601 atatggtcaa acggatattc aaatgatgaa aggggcgcgg gcgtttgctt ggcggaaggc
     3661 aggtaactca atttgaagga cgcggaagcc gatacgttaa aaaggatgaa aaatggaaaa
     3721 cgggcaacgg aataaccggt tttctttgga aaaacgaatt ttttatctgg agcattcggg
     3781 gcggtatctg atgatttgcg ccttatcgga ttattcccaa aacaaacata ctgtcgttat
     3841 ggcaaatttc atctatccgg atgaaaaaac ggattggcgg aatttggatg atttattcaa
     3901 tgagttggtt ttagaggaat tgcaggcttc atttatggat tggcatccga ctgttgaaga
     3961 ggcaatcagc cgtcatttgg aagacttttc gtaacaggcg tcaatcggaa ggggcggaat
     4021 gacggggcag gcaatcggag taaaaaatga accttgattt aaccgcgcaa aaagtccgtc
     4081 tttcttggaa ggatattctg tgggggtatg ggaataaata cttgggttgg gctgatgtgg
     4141 cagcttatgc ccgaaaaatg acgctttcag atcatgatga acgtgtgttc aaactatctt
     4201 taatcaacaa atccaatatt cttgaattaa agcctgttct ggaagatttg gcttcggaaa
     4261 tgagggatta ttcccctaaa aattggctgt acgtcctctt aagcgatgta ttccatagaa
     4321 aagaagaatt tgaggatcct ttgggggaag ttgaaaaaat ttatgcagat tttgattatc
     4381 cggaagaaat agaatcattt gtcaggtata tgccgcccaa agacggttat attccttctg
     4441 cccacaccta tgaagaaaat attgcccggt tatattctca ctgggaacac tatttgaaca
     4501 acggcggagg gcagggttaa aaccggcaat ccgatgccgt ctgaagcatt atccggcctt
     4561 cagacggcat tttgttttcc gacagtttat aaactgtcgt tgtttcttga cagaaacaac
     4621 gaccttattt atttgaaacg attggaggac atgattatgg gtttttggaa tggtgtggca
     4681 aaagcagtag gcgagggaat gattgaagca gccaatgagc ataaggcgtt gaaaatggaa
     4741 tatgcggaga aatcaagtga ggagctgcat gaaatcgtaa agagtgatgg tttttttaaa
     4801 aattccacac gggagaaagg tgcggcttat gctgttttaa aagagcgtgg cgaggtgtga
     4861 acaggaaacg gcggcatttg ccgctgtttt ttatcggcag gcatccgtct ggatgtcggg
     4921 gtaaggtttc agacggcatc gaaggttgct atgatatagt ggcttgactt taaaccggta
     4981 cggcatcccc tcgccctgtc ctgatttgaa gtgaatccgc catcatcttc ccgtcatcct
     5041 tccaaacgga atctgaaatg tccgacaacc gcctcgacgc cgcccgccgc cattccctgt
     5101 ttctcgcccg caaccttgat tccggcaaac tcaagcccga aattttcctg ccgatgttgg
     5161 acaaggtgtt gaccgaagcg gatttccaag cctttgccga ctgggacgca atccgtgcgg
     5221 aagaaaacga ggaagagttg gcgcggcagt tgcgcgggtt gcgccgttat gtggtgtcgc
     5281 agattatcgt gcgcgatatc aaccgtatca gcgatttgaa cgaagtaacc cgcacgatta
     5341 cgctgtttgc cgattttgcc gtcaataccg cgctggattt tgcctacgcc tattatcggg
     5401 acatgtacgg cacgccgatc gggcgtgata ccaaatcgcc gcagcatttg agcgtggtgg
     5461 cgatgggcaa ggcgggcggc tatgagttga acgtgtcttc cgacatcgat ttgattttca
     5521 cctatcccga atcgggcgac accgacggca ggcacgaacg gggcaatcaa gagtttttca
     5581 ccaaagtcgg gcagaaactg attgcgctgc tgaacgacat caccgccgac ggacaggtgt
     5641 tccgcgtcga tatgcggctg cggccggacg gtaattcggg cgcgttggta ttgagcgaaa
     5701 ccgcgctgga gcaatatttg attacacagg ggcgagaatg ggaacgctac gcgtggtgca
     5761 aaggccgtgt ggttacgccg tatccgaacg acatcaaatc gctggtgcgc cccttcgtgt
     5821 tccgcaaata tctggattac ggcgcgtatg aggcgatgcg gaacctacac cgccaaatcc
     5881 gcagcgaagt cagcaaaaaa ggcatggcgg acaacatcaa actcggcgcg ggcggcatcc
     5941 gcgaagttga gtttatcgcc cagattttcc agatgatacg cggcggacaa atgcgcgcgc
     6001 tgcaactgaa aggcacgcag gaaacgctga aaaaacttgc cgagctgggc atcatgccgt
     6061 ctgaaaatgt cgaaaccctg cttgccgcct accgcttcct acgcgacatc gaacaccgcc
     6121 tgcaatactg ggacgaccag caaacccaaa ccctgccgac ctcgcccgaa cagcagcaac
     6181 tgcttgccga aagcatgggc ttcgacagct atgccgcttt ttcagacggt ctcaatgttc
     6241 atcggaacaa agtcaatcag ttgttcaacg aaattttgag cgaacccgaa gagcaaacgc
     6301 aagacaacag cgaatggcaa tgggcatggc aggacaagcc cgacgaagaa gggcggcgat
     6361 gccgtctgaa ggcgcacggg ttcgatgccg aaaccgtcgc cgcaaggctc gaccaaatcc
     6421 gccacggcca taaataccgc catctttccg cacacgccca gccgcgtttc gatgcggttg
     6481 tgccgctgtt cgtacaggcg gcggcagagc aaagcaaccc gaccgataca ttgatgcggc
     6541 tgttggattt tctcgaaaac atcagccgcc gatccgccta tctcgccttc ctcaacgaac
     6601 atccgcaaac cttggcgcaa ctggcagaga ttatgggcca aagttcttgg gtggcggcgt
     6661 atctgaacaa atatccgatt ttgttggacg aactcatcag cgcgcagctt ttggataccg
     6721 cgtttgattg gcaggcactc gccgccgccc tttcagacga cctcaaagcc tgcggcggcg
     6781 atactgaagc gcaaatggac accctgcgcc gcttccagca cgcccaagtc ttccgtctcg
     6841 ccgtccaaga cctcgccgga ctgtggacgg tagaatccct ctccgaccaa ctctccgccc
     6901 tcgccgacac catcctcgcc gccgccctgc tgtgcgcgtg ggcggacatg cccaaaaaac
     6961 accgcgacac cccgcaattc gccgtcgtcg gttacggcaa actcggcggc aaagaactcg
     7021 gctacgcctc cgacctcgac ctcgtctatc tctacgacga tccccacccc gacgcaggcg
     7081 acgtgtacag ccgcctcgcc cgtcgcctga ccaactggct ttccgccgcc accggcgcag
     7141 gcagcctcta cgaaaccgac ctgcgcctgc gccctaatgg cgacgccggt ttcctcgccc
     7201 acagcatcgc cgcctttgaa aaataccagc gcgaaaacgc ctggacgtgg gaacaccaat
     7261 cccttacccg cgcccgcttc atctgcggca cgcccgaaat tcagacggcc ttcgaccgca
     7321 tccgcaccga aatcctcacc gccgaacgcg accaaaccgc cttgtcaggc gaaatcatcg
     7381 aaatgcgcga aaaaatgttc cccacccatc cgcctgccga cagcaacgtc aaatacgcac
     7441 gcggcggcgt ggtcgacgtc gaatttatcg tccaatacct catccttgcc catgcccgaa
     7501 aatatccgca acttctcgcc aactacggca acatcgccct cttgaatatc gccgccgact
     7561 gcagccttat cgacaaaaac ctcgccgaac aaagccgcac cgcctaccgc ttctaccgcc
     7621 ggcagcagca caacaccaaa ctgcgcgacg cggcaaaaac cgaagtaacc ggcgaactgt
     7681 tggcacatta cggcaatgtc aggaaattgt ggcgggaagt gttcggcgaa gaagcggcaa
     7741 ccgcctgaac aaaaaatgcc gtctgaagcc tgacaatctg ggtttcagac ggtattttcg
     7801 taccgtgccg ttttaaggtt gcggcagagc taaagcgatt tatcgggaat ggctgaaacc
     7861 caaaaaccgg attcctcttt cagttacagc agcttgatcc ctttccctta tccaacgggg
     7921 gaaggctagg atagggtggc ttgcaaatat acagaacaag ggacaagagc caccctctct
     7981 ccaaccctct ccctccgtac gggagggggt ggattctcgc gggcgaagcc cacgctacgg
     8041 ttagccttca ccccagcaca aacaattccc gcccgtgcgc cttcagccaa cttttagcat
     8101 tgtcggtatg cggcgtcagc gtgttcacca aatgccaaaa gcgcggactg tggtcggggt
     8161 ggcggaggtg gcagagttcg tggatgcaga catagtcggc gacgtattcg ggcgtgccga
     8221 tcagccgcca gttgaggcgg atgccggtgt gcgggcggca tacgccccaa aaggttttgg
     8281 cgttgctcag gtctgtggcg gtgggcgtca gtcctgtttc ggctgcgtgt ttttcaaggc
     8341 ggggcagcag gtattcgcgg gcgcgttcgt tcaacaggcg gcgcaggtgg tcgatttgtg
     8401 cggcggtttc ttttcgggga agcaggattt cagacgacgt gatacggata tggctttggc
     8461 tgtgggtatc cagcttggtc tttattcccc gataccaaat ccactcgggt aagtttgggc
     8521 gggaaacagg atgcacgggc gttttggcaa gcgtgttccg caaaatcgtt tcgtttgccg
     8581 ccagccagtt tgctaacgcg tggtcttgaa aaaagggtgg gacgttgatg ctgaccgtct
     8641 gcatattgac ggggcgcaga atcagatttt tcttggcact gcgtttgagt tcgatttcga
     8701 tgcacaaacc gtcggaaaga gtataggtga agcgtttcat agttgtgaat gggtttcaga
     8761 ccggatacat cgtctgaaac aggaattttc catatcaggc ggcaaacttc ggataatata
     8821 caaaatcaaa catctgcgct acaaggttca gccgaacaag ccgccgatat atttgctgat
     8881 ggtaatggcg ctgagtactg ccatcaaacc gaccacaatc acgccggaaa cggtgagcca
     8941 cagcgggtgt ttgtagtcgc cgacaatttt ggttttgtag gcggcaatca gaatcagacc
     9001 gagggaaatc ggtaaaatca ggccgtttaa tgcgcctacg aacaccagca cctgcgccgg
     9061 tttgccgatg gtggaaaata cggcggtgga cacggcgata aaggcaataa tccatttgtt
     9121 tttattgcgt tcgatagacg ggctgagacc ggagaagaac gacaccgaag tataagccgc
     9181 accaatcacc gaagtaatcg aagccgccca aatcaccacg ccgaaaatca gcaggccgat
     9241 gtatcccgcc gcatattcaa acggtgtgga agcagggttg tcgggattga gctgtacgcc
     9301 ttggctgacc acgcccaaaa ccgccaaaaa caatacaatc cgcataatcg aggcaatcag
     9361 gatcgcccgc accgagcttt ggctcacttc cggcaacgcc gatttgcctt tgatgcctgc
     9421 gtccagcaga cggtgcgcac cggcgaaggt gatgtagccg ccgaccgtgc cgcccaccag
     9481 tgtaacaatc gccattgcat cgagtttttc cggcataaag gtatgcacgg cggcatctgc
     9541 cagcggcgga ttcgcctgcc atgccacata aaccgtcagc gcaatcatta cgaaacccat
     9601 cacttgggcg aatttgtcca tcactttgcc tgcttcttta aacagaaaca caccgatggc
     9661 aatcacgccg ctgatcacgg caccggtttc cggcgacagt ccggtcagca ggttcagacc
     9721 caagcctgcg ccgccgacgt tgccaatatt gaacgccaaa ccgcccatca caatcagcac
     9781 agccaagaaa tagcctgcgc cgggcaagac ctgattggca atatcctgcg cctgtttttc
     9841 ggaaacggcg acaatccgcc aaatattgag ctgcgccccg atgtcgagca gaatcgagag
     9901 cagaatcaca aagccgaaac ttgccgccag tgcttgggtg aaggtggcgg tttgggtcag
     9961 aaagcccggg ccgatggcgg aagtcgccat caggaatgca gcgccgatta aggcatttct
    10021 gcggtttttt tgattagaca taatcgctta tcctctataa aattggttgt tgctgtgttt
    10081 gggcgaaacc tgcggtttta gctacgcaga aactcgcttt gctcgttttg gcgaaacctg
    10141 cggttttcag acggcctatg aactgttttt caagcagaaa ctttgatgcc tgccgccagt
    10201 agttcctgcc ggattttttc ggcaaacacc acggcgtgcg gcccgtctcc gtgcagacag
    10261 atgctgtcgg cttgcacggc aaccaggctg ccgttcactg ctttgacccg cccgtcccgc
    10321 accatctgca atacttgggc gatggcttct tcgtcgctgt ccacctgcgc atcggggcgg
    10381 ctgcggggaa ccagcgtacc gtcgggcata tagcggcggt cggcgaatac ttcggaaatc
    10441 acacccaagc ctgcggcttt tccggcttcc aagagcaggc tgccggaaag tgccatcaat
    10501 ttcaatttcg ggtcgaaatc cgccacaatt cgggcaacgg tatccgccag cgcacggttt
    10561 ttcgccgctt ggttgtacat tgcgccgtgc ggtttgacat aagccatttc caaaccctga
    10621 tcacggcaca aggcctgcaa tgcgcccaac tggtaattca gacacgcccg caaatcggct
    10681 tcggacagat tcatttcggt acggccgaag ttttcccgat cgggatagcc ggggtgtgct
    10741 ccgatgcgca cgccgttttg ttgggcatac gccaatgccg cccgaatatc ggcaatgctg
    10801 ccggcgtgtt gggcgcaggc gatgttggcc gaagtaatca gctgcaacaa ggcttcgtcg
    10861 ctgccgcagc cttcggcgag atcggcgttt aaatcaacct gcttcatggg tgattctccg
    10921 tatttggttc agataggctt ggtttttgcg ccgcagggcg gtggcttctt tcaagccgat
    10981 tattttgaat ttgactttgc tgccgaagcg cacctgtgcc agcctgccca aatcggcggc
    11041 ggcaacggta gcgattttcg gataaccgcc ggtggtttgt gcatcggcca gcaggataat
    11101 cggtttgccg ccgggcggca cctgcacggt tcctgcctga acagcgtggg acagcatttc
    11161 caaaggttgc gacagggtca gcggctgtcc gtcgaagcgg tagcccatgc ggttgctatc
    11221 gctttgcagc gtccacgttt cccgttccag attcagacgc cctttttcac tgaaagcggc
    11281 atattccgac gaaggaacaa ggtggacggt atcggtaaac ggtatcgggg caatgccgac
    11341 tttggacaat tcctgcgcac ctttgccgat ggggagacaa tcaccttttt gcagcattct
    11401 gccctgatgg ccgccgaaac cggctttcag gtcggtgctt ctcgaaccca tcacttccgg
    11461 cacatcaaat ccgcccgcca cgcacacata gccgtacatg ccctgcacgg cacgcaccat
    11521 tttcaaggtc tgccctttgc gggcggtata acgccaatac gaatagaccg gttcgccgtc
    11581 caattccgcc tgatacacgg caccggtgag acaaaacggc gtatcccgtt caaacaccag
    11641 cattatcccg cccaaagcga tttcgattgc ggccgtgcct tcgtcgttgc ccaataaaat
    11701 attacccgcc gccaaagcaa ccgtgtccat cgcaccggca tgaccgatgc cgtaacgccg
    11761 gtgtccgtag cgtccggtat cctgaatatg cgccggtgcc tgcactgccg aaacgtgaat
    11821 catggctcaa tcctttctgc aacaaagcgg acttggtcac ccgccgccag cagggtcggc
    11881 ggattcaaat cggctcggaa caagggtaat tcggttctgc cgataatctg ccagccgccg
    11941 ggcgaagcga acggatacac accggtctga ctgccgccga taccgaccga accggcagga
    12001 acggacgttc tcggcacggc acggcggggc gtgtgcaatg cttcgggcaa gccgcccaga
    12061 taagggaaac cgggctggaa gcccatcata aatacggtat aagtttgcgc cgtatggcgg
    12121 cggacgattt cggaaataac cgtctgatgg aaagcagcga cttccgccaa atccgggccg
    12181 tattcgccgc cgtagcagac gggaatttcc accagtttgc cctgatggtc tgtaacggcg
    12241 gtgtgttccc acacatattg caattcatcg gcaagcgtcg ccaaatcggt atcgaaacgg
    12301 gtgaacacgg tcagattgtt catgccgacc accacttcct caatcctgtc gtgctgcccg
    12361 agcgcagcgg caaacgccca caacttttgc tgtttgccca gttcggaagg cgcattcagt
    12421 cggcagacca aagcggattc gctgattggt gtgatctcta ttctcatttg ttgttcattt
    12481 tggttatgtt ttaatgaatc tatatgcagg ggcggcggtt tgtcaatatc ttctgtgctg
    12541 catcatcaaa ccgtcgattg gaaaagtgct gccctgccgc tgcacttttt cagacgacct
    12601 taaaccgttt ctattaaaat agcgcattcc acttttcaga cggcatcctt atgtttcccg
    12661 accaatccgc ccccaacctg ctgcaaggct tgaatcccga acaactctcc gccgtaacct
    12721 ggccgccgca atccgccctt gtgctggcgg gcgcgggcag cggcaaaacg cgcgtgctga
    12781 ccacgcgcat cgcatggctt ttgcaaagcg gacaagccag cgtgcacagc attatggcgg
    12841 taacgtttac caacaaagcc gccaaagaaa tgcaaacccg actcggcgcg atgattccca
    12901 tcaacgtccg cgccatgtgg ctcggcacgt tccacggtct ctgccaccgc tttttgcgcc
    12961 tgcaccaccg cgacgccggt ctgccgtctt cctttcaaat cctcgacggc ggcgaccagc
    13021 tttccctcat caaacgcctg ctcaaaagcc tcaacatcgc cgaagaaatc atcgcgccgc
    13081 gttcgctgca aggctttatc aacgcgcaaa aagaatccgg cttgcgcgct tccgtcttga
    13141 gcgcgcccga cccgcacacg cgccgcatga ttgagtgcta cgccgaatac gacaaaatct
    13201 gccaacgcga aggcgtggtc gatttcgccg agcttatgct ccgcagctac gaaatgctgc
    13261 aaagcaacga aatcctgcgc caacactacc aaaaccgctt caatcacatt ctggtcgacg
    13321 aattccaaga caccaacaaa ctgcaatacg cctggctcaa actcatggcg ggcggcaacg
    13381 cggcggtatt tgccgtcggc gacgacgacc aaagcatcta ccgtttccgt ggcgcaaacg
    13441 ttggcaatat gaccgcgctg atggaagaat tccacatcga cgcgcccgtc aaactcgaac
    13501 aaaactaccg ctccgtcggc aacatcctcg ccgccgccaa cgccgttatc gaaaacaacg
    13561 acgaacgcct cggcaaaaac ctgcgcaccg acgccgaagc aggcgacaaa atccgctact
    13621 actccgcctt taccgacctc gaagaagccc ggttcatcgt ggacgaaacc aaagccctcg
    13681 aacgcgaagg ctgggatttg gacgaaatcg ccgtcctcta ccgcagcaac gcccaatcgc
    13741 gtgtcatcga acaaagcctg ttccgcagcg gcatccccta caaaatctac ggcggcttgc
    13801 gcttttacga acgccaagaa atcaaacacg cgctcgccta cctacgcctc gccgtcaatc
    13861 ccgacgacga caacgccctt ttgcgcgtca tcaacttccc accgcgcggc atcggtgcac
    13921 gtaccgtcga aaatcttcag acggcctcaa acgaacaagg catcaccctc tggcaagccg
    13981 cctgcaacgc cggcgcgaaa gccgccaaag tcgtcgcctt cgtccgcctg attgaagccc
    14041 tgcgcaacca agtcggacaa ctgtccctgt ccgaaatcat cgtcggcatc ctcaaagaca
    14101 gtggcttgac cgaacactac cgcacccaaa aaggcgacaa ccaagaccgt ctcgacaacc
    14161 ttgacgaact cgtcaacgcc gccatcgagt tcaaacccga agacagcaac ttcgaaatcc
    14221 tgcctgaaaa catttcagac gatcccgcct tccccatcct cgctttcttg agcaacgcca
    14281 ccctcgaatc cggcgaaaat caagcaggtg caggcgaaaa agccgtccaa ctcatgaccg
    14341 tccacgccgc caaaggcttg gaatttaacg ccgtcttcct caccggcatg gaagaaggcc
    14401 gcttccccag cgaaatgagc cttgccgaac gcggcggcct cgaagaagaa cgccgcctca
    14461 tgtacgtcgc catcacccgc gcccgcaaac gcctctacat caccatggcg caacaacgca
    14521 tgctgcacgg acaaacccaa ttcggcatcg tctcccgctt cgtcgaagag atcccacccg
    14581 aagtattgca ctacctgtcc gtcaaaaagc ctgcctacga cagttacggc aacacgcgcc
    14641 aaaccgccgc atccaaagac aaaatcatcg acgactacaa acagccccaa acctacgcag
    14701 gtttccgtat cggacaaaac gtccgccacg ccaaatttgg caccggcgtg attatcgatg
    14761 ccgcagataa aggcgaatcc gcccgactga ccatcaactt cggcaaacag ggcgtgaaag
    14821 agttggatac caagtttgcg aaattggaag cgatgtaaat ttgaaatgca ggctggatac
    14881 tcgtatccga caaaaatatt tgacgcgtct atcgtttccg aaaaaccgct gttggaaaat
    14941 gtcggattcg agaatccgac ctatggcaaa aaactgtagc aaggacaagg caaaaggccg
    15001 tctgaatccg gtaacaacaa attttgccga aacctccgcc gtcattcccg cgcaggcggg
    15061 aatccagacc tttcggcacg ggaacttatc ggataaaagg tttctttaga ttccacgttc
    15121 tagattcccg cctgcgcggg aatgacggac ggtaaaagcc gggtatagat acctaaccct
    15181 tgtttatagc tgaaaatcaa taaggaagaa cattatgtcc caagttttta aagattttga
    15241 tttgtcctcc gtatgggaat ccgacagttg ggcagatgaa aactacaaag aagccccgtt
    15301 tacccctgaa attttggctg ccgtagaaag tgaactgggc tataaattgc cgcaaagttt
    15361 tattgaattg atggcggtac aaaacggcgg aatatttgtc aaaaactgtt ttccgaccac
    15421 acagagaaat tcgtgggcga aagatcatgt gcaaatttgc gaggtatcgg gcatcggctt
    15481 tgaaaaagaa gggagtttgt gcggcgaaac agggcaaaaa ctttggatgg aagaatggga
    15541 atatccgccc atcggcgtgt attttgccaa cgacccgtca ggcggtcatg ccatgtttgc
    15601 cttggactat cgggagtgtg gaaaagacgg cgagccgaaa gtggtgtttg tcgagcaaga
    15661 atcggatttt gaaatcgtcg aacttgcccc cgattttgaa acctttatcc gcagcttgcg
    15721 gcatgaagat gagtttattg acgaggaaat ataaaacggc ggttgaaaaa ccggaatcat
    15781 caagggaaaa cgggcgaaat aacggataat cgcttgaatc cgtaaggaaa acggtttggt
    15841 ggaacgcgcc atccaagacc tttgcaaaga aaaactgtct ccgacagcat tgacattatt
    15901 aacagaactt atcaagtttg gagctatctc aaatataatt cggttatcct tttgtatcca
    15961 ttaaatcata tgcttcaatt aattgttgtt ctagctcttg taccaatttt gaattgcgaa
    16021 ttcctgacac aatctcaaat tcttctgcat ctatgcaaac acctgcataa atttcaataa
    16081 caagggaacg caataattga agctcttctc ttgttaaaga aataataatg tcatcacctg
    16141 tgtaattgat tatattcata ataattttat ttttgtttgt caaagtaagt tttgcctaag
    16201 gttggtctaa atgcagttcc accatctttt gaatttgggt ctctgattac aattgctcca
    16261 gacttatcat cccaaattgc tcttatgtgt ttggattgta atcttcgaat tcccaagaaa
    16321 aaaatcgtaa taagtttgaa agtgtcaaat cccaagtttc ttttgagcaa tattctaata
    16381 ttttatcaat ttcactttta ataatcttat gatcaaactg ttctaatatt aatgcattag
    16441 accaaaaaaa accttcttta ttacaatgat gggaaatcca tttaggagaa caaatgcaaa
    16501 gtgaaaaaat agatgagcct tgttctcctt cgattccgat atccaaatct atccatctat
    16561 ggaaattatc tggaatttcg ggggtaaatt tttcaaaatc aatatcatat aaatttatgc
    16621 tttttaaatc caatttaatc attagggctg tcctagataa atagggaaat tcaaattaag
    16681 ttagaattat ccctatgaga aaaagtcgtc taagccggta taaacaaaat aaactcattg
    16741 agctatttgt cgcaggtgta actgcaagaa cagcaacaga gcctgacagc attgtttata
    16801 cggattgtta tcgtagctat gatgtattag atgtgagcga atttagccat ttccgtatca
    16861 atcacagcac acattttgct gaacgacaaa accatatcaa tggaattgag aatttttgga
    16921 accgggcaaa acgtcattta cgcaagttta acggcattcc caaagcgcat tttgagctgt
    16981 atttaaagga gtgcgaatgg cgttttaaca acagtgagat aaaagttcaa atttccattt
    17041 taaaacaatt agtaaaatcg agtttatctt agttgtccag gacagcccca ttatttttat
    17101 aacaccgtga agccgcacag cagtttgaac agtgatacgc cgtttgcggg cttacgagtt
    17161 tattttcccg gcctgcagtt tgagcaatac ggtgatttcc tacggttaat acaaatgttt
    17221 acacattgat acatttcatt tatagttccg cctatttgaa aatagaaaat atgaattcga
    17281 ccgcaagtaa aaccctgaaa ggattgtcgc tggtgttttt cgcctctgga ttctgcgccc
    17341 tgatttacca ggtcagctgg cagaggcttc tattcagtca cataggtatc gatttgagtt
    17401 cgattactgt cattatttct gtatttatgg tcggcttggg tgtaggtgcg tatttcggtg
    17461 gacgcattgc tgaccgtttt cctgaaagta tcatccctct gttttgcatc gctgaagcat
    17521 ccatcggtct gttcggtttg gtaagcaggg gtctgatttc cggcttgggg catcttttag
    17581 ttgaggctga tttgcccatc atcgctgctg ccaatttcct cttattgctg cttcctacct
    17641 ttatgatggg cgcgaccttg cccttgctga cctgtttttt taaccggaaa atacataatg
    17701 ttggcgagtc tatcggtacc ttatattttt tcaacacttt gggtgcggca ctcggatcgc
    17761 ttgccgccgc cgaatttttc tacgtctttt ttaccctctc ccaaaccatt gcgctgacag
    17821 cctgctttaa ccttctgatt gctgcttcag tatggctgcg ttacagaaag gatggatata
    17881 gtgaacacta aaccgaatac tagtttgatt tatatgcttt ctttccttag cggcttattg
    17941 agcttgggta tagaagtctt gtgggtaagg atgttttcgt tcgcagcaca gtccgtgcct
    18001 caggcatttt catttaccct tgcctgtttt ctgaccggta tcgccgtcgg cgcgtatttt
    18061 ggcaaacgga tttgccgcag ccgctttgtt gatattccct ttatcgggca gtgcttcttg
    18121 tgggcgggta ttgccgactt tttgattttg ggtgctgcgt ggttgttgac gggtttttcc
    18181 ggcttcgtcc accacgccgg tatcttcatt accctgtctg ccgtcgtcag agggttgatt
    18241 ttcccgctcg tacaccatgt gggtacggat ggcaacaaat ccggacgaca ggtttccaat
    18301 gtttatttcg ccaacgttgc cggcagtgca ttgggtccgg tccttatcgg ctttgtgata
    18361 cttgatttct tgtccaccca acagatttac ctgctcatct gtttgatttc tgctgctgtc
    18421 cctttgtttt gtacactgtt ccaaaaaagt ctccgactga atgcagtgtc ggtagcagtt
    18481 tccctaatgt tcggcatcct catgttccta ctgccggatt ctgtctttca aaatattgct
    18541 gaccgtccgg atcggctgat tgaaaacaaa cacggcattg ttgcggttta ccatagagat
    18601 ggtgataagg ttgtttatgg ggcgaatgta tacgacggcg catacaatac cgatgtattc
    18661 aatagtgtca acggcatcga acgtgcctat ctgctaccct ccctgaagtc tggcatacgc
    18721 cgcattttcg tcgttggatt gagtacaggt tcgtgggcgc gcgtcttgtc tgccattccg
    18781 gaaatgcagt cgatgattgt tgcggaaatc aatccggcat accgtagcct tatcgcggac
    18841 gagccgcaaa tcgccccgct tttgcaggac aaacgtgttg aaattgtatt ggatgacggt
    18901 aggaaatggc tgcgtcgcca tcctgatgaa aaattcgacc tgattttgat gaatacgact
    18961 tggtactggc gtgcctattc caccaacctg ttgagtgcgg aatttttaaa acaggtgcaa
    19021 agccacctta ccccggatgg tattgtaatg tttaatacca cgcacagccc gcatgctttt
    19081 gctaccgccg tacacagtat tccctatgca taccgctatg ggcatatggt agtcggctcg
    19141 gcaaccccgg tagttttccc taataaagaa ctgctcaagc aacgtctctc ccggttgatt
    19201 tggccggaaa gcggcaggca cgtatttgac agcagcaccg tggatgctgc agcacaaaag
    19261 gttgtctctc gtatgctgat tcagatgacg gaaccttcgg ctggggcgga agtcattacc
    19321 gacgataata tgattgtaga atacaaatac ggcagaggga tttaaccgtc ttaaagggtt
    19381 tcaggcaacg caggttttag gtaacgtcct gctagttcaa aaaaaccgca tcacagcagt
    19441 cgggacaaaa tggtttaaac attttgtccc gaattcttat tcctat
//
LOCUS       DAXHTY010000034        19332 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXHTY010000034 DAXHTY010000000
VERSION     DAXHTY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.034"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..101
                     /locus_tag="ACFNMK_000986"
     CDS             <1..101
                     /locus_tag="ACFNMK_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HFC6364275.1"
                     /translation="TFACVLGTLVASEHGATPDISAERIGRFMNPV"
     gene            complement(161..718)
                     /locus_tag="ACFNMK_000987"
     CDS             complement(161..718)
                     /locus_tag="ACFNMK_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6364276.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(794..2428)
                     /locus_tag="ACFNMK_000988"
     CDS             complement(794..2428)
                     /locus_tag="ACFNMK_000988"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6364277.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKEFLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2498..2831)
                     /locus_tag="ACFNMK_000989"
                     /pseudo
     CDS             complement(2498..2831)
                     /locus_tag="ACFNMK_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2831..3937)
                     /gene="serC"
                     /locus_tag="ACFNMK_000990"
     CDS             complement(2831..3937)
                     /gene="serC"
                     /locus_tag="ACFNMK_000990"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC6364278.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4302..4733
                     /gene="rimP"
                     /locus_tag="ACFNMK_000991"
     CDS             4302..4733
                     /gene="rimP"
                     /locus_tag="ACFNMK_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC6364279.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4761..6263
                     /gene="nusA"
                     /locus_tag="ACFNMK_000992"
     CDS             4761..6263
                     /gene="nusA"
                     /locus_tag="ACFNMK_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC6364280.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6275..9163
                     /gene="infB"
                     /locus_tag="ACFNMK_000993"
     CDS             6275..9163
                     /gene="infB"
                     /locus_tag="ACFNMK_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC6364281.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSEKPAESKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKQQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPI
                     VVAVNKIDKDTANPERIRQELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
     gene            complement(9375..10769)
                     /locus_tag="ACFNMK_000994"
     CDS             complement(9375..10769)
                     /locus_tag="ACFNMK_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC6364282.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLETHTDTRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTYRPETSRTERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(10843..12183)
                     /locus_tag="ACFNMK_000995"
     CDS             complement(10843..12183)
                     /locus_tag="ACFNMK_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC6364283.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVAAATFAFFTGFSSTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGRDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRLVEMLAWLPS
                     KLGFPVPDARAVVEGRLNGNIADAQAWSGLLVGSIACYGILPRFLAWVVCKILLKTSK
                     NGLDLEKPYYQAVIRRWQTQIVDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGA
                     WFEGRLAQEWLDKGVATNREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLGHWRNALAECGAAWLEPDRAAQEGRLKDQ"
     gene            12326..12952
                     /locus_tag="ACFNMK_000996"
     CDS             12326..12952
                     /locus_tag="ACFNMK_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC6364284.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLVLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13016..14434)
                     /locus_tag="ACFNMK_000997"
     CDS             complement(13016..14434)
                     /locus_tag="ACFNMK_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6364285.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            14761..15489
                     /locus_tag="ACFNMK_000998"
     CDS             14761..15489
                     /locus_tag="ACFNMK_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC6364286.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     SNPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15548..16036)
                     /locus_tag="ACFNMK_000999"
     CDS             complement(15548..16036)
                     /locus_tag="ACFNMK_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC6364287.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(16795..17259)
                     /locus_tag="ACFNMK_001000"
     CDS             complement(16795..17259)
                     /locus_tag="ACFNMK_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC6364288.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            17584..18642
                     /gene="alr"
                     /locus_tag="ACFNMK_001001"
     CDS             17584..18642
                     /gene="alr"
                     /locus_tag="ACFNMK_001001"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC6364289.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT         5110 a         5281 c         4853 g         4088 t
ORIGIN      
        1 agacctttgc ctgcgtactt ggtacgctgg tcgcctccga acatggcgcg acacccgaca
       61 tttccgccga acgcatcggg cgtttcatga atccggttta aaacgcatgg aaaaatgccg
      121 tctgaaagcc tttcagacgg catttcgctt gagattccgt ttaccaatgg ctgacaaacg
      181 cttccaaatc ggtattcttg ggcttatgca cttcctctgt cggcgtgccg accatcatca
      241 gcccgatgat tttatcctta tccgcacatc cgaaagcctc acgcaacagg gggctgttga
      301 cccacatccc cgtaatccag acattgtcga aaccttgcgc cgttgccgcc agttgcagcg
      361 catacgccgc acaacccgcc gtcagcatct gctcccattc cggtttcggc ttaggcacat
      421 cgcggttcgg cgcaaacgtt acaccgataa ccatcggcgc catattgccc actttttccg
      481 cctttttcat cgcatcgtcg ccgaaattca attcggcaac cgtttgcttt aacacatcgc
      541 gaaaacgttg caatcctacc tcgccttgaa tcacggtaaa acggaagggg cgcatattgc
      601 cgtgatcggg aacttgggtt gccgcctgaa atatttgttc caactccgcc gcatcggggg
      661 cggggtgctt cagctttttg gaagatcggc ggttcgtcaa taattttaaa gcatccatat
      721 cgttatactc cggtcatcgg tcgggtgttc ggacacatat gccgtccgaa ggcttcagac
      781 ggcatatccg gcatcagcgc ggacggcggc aggctgccaa tatatccatt tccttccgat
      841 aggtttggct gttggaaatg tccatcagcc ccaataccgt gctgaaatag tggtcgtgcg
      901 aatattcgtt ttccgccgct ttttgtttga ggcattggaa atctatgccc ccgtgttggc
      961 ggaaggcttt ggaaaaccac ataaccatcg ggatatgcgt ctgcccggaa ggcgcgatgg
     1021 cgtaaggcgc ggcgtgcagg tacatcccgt tttcgcccaa actttcgccg tggtcggaaa
     1081 cataatgcac cacgctttcc aaatcgtcgc ggttttcaag tttgcggata accttgtcga
     1141 taaactggtc cacatacaaa accgtattgt cgtaagtgtt gaccagcgtg gcgcgggtgc
     1201 atttgttgat ttcgttggtg tcgcaggtcg gcgtgaattt gcgttcggct tcggtatagc
     1261 gttcgtaata cgtcggcccg tggctgccga tggtatgcag gattaaaacc gcgtctttat
     1321 cgtttttgtt gaggacttcg tcgaacttag tcagcaggat attgtcgagg cactcgccgt
     1381 tgcggcagta ttcgggcagg ttgagcgagg taacgtcggt attcggcact ttgccgcaca
     1441 cgcccttgca gccggaatcg ttttccaacc aagtaacttc cacgccggcg cgctgcacga
     1501 tgtccagcag gttgtcttgg tgttcggctt tgatttcgtc ataatccgtg cggtcgaagg
     1561 ttgagaacat acacggcagg gagtgcgcgg tcgatgtgcc gcagcttctg acctgcggga
     1621 aattgacaat ttcatcgccg cgcgcggcaa gcagcggcgt agtttggcgg ctgtaaccgt
     1681 tcaaacccca gttggcggca cgcgtggtct cgcccacgac cagcaccacg aaacggcgca
     1741 ggctgccggc cggccggttt tgcacgaccg ccatatccaa ttgcgtataa ggaatattgg
     1801 aacgcttcca atctttgtat ttcgacacgc ccgcgccgat gaaattagac ggcacaatca
     1861 gatgggttac tgatttattg ttgcggaaaa acgaggcgta atcctgatat tgcaacattg
     1921 cgatgcccaa cgcgcacaaa aaggaaacgg cggcaagcac aaggcgcgtc aaaaactcct
     1981 tataccaaac gcggtattta accttgacgg cgatatacgc cagcgcgggc aatacgccca
     2041 aacatacaat ccacagcaca tagcccggcg taatcaggcg cgcgctttcg gcagccgtag
     2101 tttgcaagac attattcaac atcgacttgt tgaaatagat attgaaaaat atttcttggt
     2161 aagacaccgc cgcactgata accaatatca acggaatcaa taccttatgc acgaaaggca
     2221 gggcaatgac gtgaaaaacg aaattactta aaaaaaacag caccaccggc atcgtataga
     2281 ggaagatatc cgccccggtg ccgttaaaag gatgaagctc gacaactttg gcaaaaaagg
     2341 cgtaattcaa taccagcgag gaatacaggg aaaggaaggc aatcagcgcg gaagagccga
     2401 gcttcggcct caggttcggt tttatcattt ggaatgtgtc ggataagggt tggaaaaggc
     2461 atccggcatt tggaatccgg attattgaaa aagattctta attataaggc aacggagcaa
     2521 agcagggcaa gaaaacggcg gctgtgcggg gggttccgcc cgccattcaa acgtccggca
     2581 gacataaaaa catcgtaagc aagattcgaa ccggtctgca accgcccctg ccagaaaaac
     2641 gggcaaagca tttcatattg gaaaaacccg gccgcgccgc cgacgggaca gtccggcaca
     2701 aacagcatca cgctcggatt gaaaaggacg gatagccgcc ggcagcacaa tcttaccacc
     2761 tccaaaacgc cgccggagaa cgccgaaaca gccgatgccg tctgaagccg cttcagacaa
     2821 catcgggaca tcaaccgtaa cgccgttgga aatcgcgcat aaaatccgcc aaagcccgca
     2881 cgccttcaag cggcatcgca ttataaatgc tggcacgcat accgccgacg gttttatagc
     2941 ccttaagcag gcacaagccc tgcaattcgg cttccagcac aaaacggcgg tcaagctcct
     3001 catcccccgt ttggaacacg acattcattt tagaacgcgc attcggacgg atacggttga
     3061 tatagaaacc gccgctgccg tctatcgtct catacaaggt ttgcgccttc agccgattga
     3121 ccgcttcaat ttttttcaca ccgccctgcg cctgcagcca gcggaacacc agccccgaca
     3181 tataaatcgc gtaagttgac ggcgtgttgt acataccttc gcggttcaaa tgcgaacggt
     3241 agttgaacac atcgggaata tcgttcggac aacgctcgag caaatcctca cgcacaatca
     3301 ccaccgtaac ccctgccggc ccgatgtttt tctgtgcgcc tgcgtaaatc agtccgtagt
     3361 cggcaacatc aaactcgcgc gacaaaatct cgctggacat atcgcacacc agcggcggca
     3421 tgccttctga aaggcgcggc acttcacggt attgcagccc gttgaccgtt tcattgacgg
     3481 caaaatggac aaacgccgaa tcgggtgcaa catcccacgt ttccacaggc ggcaggtcga
     3541 gatagtcgaa ctgctcgccg ccatgcgccg ccaaacggat ttccgtatcg gtcaaacggc
     3601 tcatctgttc ataagcgata cggctccagt tgcccgttac caccgcgtcg gcagtgcgga
     3661 aaccgtgtgc cagattcatg gctgccatat taaattgggt tgttgctcca ccctgcagaa
     3721 acaatatctt atagttgtca ggcactttca aaagctgcct caaatcctgt tccgcatgat
     3781 gcaggatgct caaaaacatt tccgaacggt ggctcatcgc catcacagga aaacccgtac
     3841 cgttgtagtc caacatttcc tgccgcgccg tttccaacac ggcttcgggc aatacggcag
     3901 ggccggcgga aaaattgtaa atcggataaa gagacataat gcagccttga ttctgaacaa
     3961 taacccgccc gattttaggc ttgcagcagg ctttgtgcaa ggatgggaaa tacctgtcct
     4021 ccgccccgct tcatgccgcc cgaacacgga aaacaacatc aatatattga tttacaaata
     4081 taaaaatcat gctagcgaca aatagataca tttgttttgt caacaatatt cacgatttcc
     4141 cattacaaac ctcccttaca cccgcttttt tccgtcccaa aaacacaaaa taaatcaaca
     4201 ctttcatttc tccgcaaaag cggttataat cacgccgatt tttcaacttt gacgaaaaat
     4261 ggccacgggg ccattttttt attatgtaca tagggagcag catggatatc caaaccatcc
     4321 tcgaaaaaac cctgcccggc ctgggctacg aactggtcga tttcgaactg accgcgcaag
     4381 gaacattgcg cgtgttcatc gacaaagaaa gcggcattac cgtcgaagac tgcgcaaccg
     4441 tcagcaacca cttgagccgc gtcttcatgg ttgaagacat cgactacaaa aacctggaaa
     4501 tttccagccc cggactcgac cgccccttga aaaaagccgc cgacttcgtg cgctttgccg
     4561 gtcagaatgc caaaatcaaa acccgcctgc cgatagacgg tcagaaaaac tttatcggta
     4621 aaatcgaagg ctgcgaaaac gataccgtta ccgtatcctt cgacggcaaa accgtacaaa
     4681 tcgaattggg caacatcgac aaagcccgtc tgcgccccga attcaaattc taaaacacaa
     4741 caatattgga gatgttcaaa atgagtcgtg aaatgttaca gctggcagaa gcactggcaa
     4801 gcgaaaaaaa cgttgatgcg gaagtcgtct tccaagcact ggaattcgcc ctgtctaccg
     4861 ccgccaagaa aaaggcagac cgcgaacaca tggacgtgcg cgtccaaatc aaccgcgaca
     4921 ccggcgaata ccaaaccttc cgccgctggc tgattgtcgc cgatgaagac tatacctatc
     4981 ccgatgtcga aaaaaccatc gaggaaatcc aagaggaaat tcccggcact accatccaaa
     5041 tcggcgaata ctacgaagag cagctgccca acgaaggctt cggccgccaa gccgcgcaaa
     5101 ccgccaaaca aatcatcctg caacgcatcc gcgatgccga gcgcgagcag aatctgaacg
     5161 agtttctcgc cgtcaaagaa gacatcgtgt ctggcacggt caaacgcgtc gaacgccacg
     5221 gcatcatcgt cgaagtcgtt gccggcaaac tggacgcgct gattccgcgc gaccaaatga
     5281 ttccgcgcga aaacttccgc agcggcgacc gcatccgcgc cctcttcctg cgcgtcgaag
     5341 aaatcggcaa caccggccgc aaacaagtca tcctgagccg tacttccggc gatttcctcg
     5401 tcaaactgta cgccaatgaa gtacctgaaa ttgcagacgg catgcttgaa atccgcgctg
     5461 tcgcccgcga cccgggccag cgtgccaaag tcgccgtcaa agccaacgac cagcgcatcg
     5521 atccgcaagg cacctgtatc ggcgttcgcg gttcgcgtgt caatgccgtc agcaacgaat
     5581 tgtccggcga gcgcatcgac gttgtccttt ggtcgcccga gcctgcccaa ttcgtcatga
     5641 gcgcgctctc acccgccgaa gtcagccgca tcgtcatcga cgaagacaaa cacgccgtcg
     5701 atgtcatcgt tgccgaagac cagctcgcgc tcgccatcgg acgcggcggt caaaacgtgc
     5761 gccttgcttc cgacctgact ggctggcagc tcaacatcat gacttccgcc gaggcagacg
     5821 aacgcaatgc ggcagaagat gccgccatcc gccgcctgtt tatggatcac ttgaacgtgg
     5881 acgaagaaac cgccgacgta ctggttcagg aaggttttgc aaccttggaa gaagtcgcct
     5941 atgttcctgc cgccgaactg cttgccattg aaggatttga cgaagaaatc gtcgatatgc
     6001 tccgcaaccg cgcccgcgat gccatcctga ccatggcgat tgccgccgaa gaaaaactgg
     6061 gcgaagtgtc cgacgatatg cgcaacctcg aaggcataga tgccgatatg ctccgcagcc
     6121 ttgccgaagc aggcattacc acccgcgacg acttggcaga gcttgctgtg gacgaactga
     6181 ttgaaatcac cggtgtaaac gaagaaaccg caaaagccgt catcctgacc gcacgcgaac
     6241 actggtttac cgaagacaaa taaagggggt acagatgagt aacacaaccg tagaacaatt
     6301 tgccgccgag ctgaaacgcc ccgtcgaaga cctgttgaaa cagttgaaag aagccggcgt
     6361 cagcaaaaac agcggcagcg attccctgac gctggacgac aaacagcttc tgaacgccta
     6421 cctgaccaag aaaaacggca gcaacggcgg taccatcagc atccgccgca ccaaaaccga
     6481 agtcagcacc gttgacggcg taaaagtcga aacacgcaaa cgcggacgca ctgtcaacat
     6541 tccttctgcc gaagaattgg cagcacaggt aaaagccgcc caaacccaag ccgcacctgt
     6601 ccggccggag cagacggcag aagacgcggc aaaagcccga gccgaagctg ccgcacgtgc
     6661 agaagcccgt gccaaggcag aagcggaagc ggcaaaactg aaagcggcaa aagcaggcaa
     6721 caaagccaaa cctgccgcgc agaaacccac cgaagcaaaa gccgaaaccg cacccgttgc
     6781 ggcggaaacc aaacccgccg aagaaagcaa agcggaaaaa gcccaagccg acaaaatgcc
     6841 gtctgaaaaa cccgccgaat ccaaagaaaa agccgccaag ccgaaacacg agcgaaacgg
     6901 caaaggcaaa gatgccaaaa aaccggcgaa acctgccgca cctgccgtgc cgcaacccgt
     6961 ggtcagcgca gaagaacagg cgcaacgcga tgaagaagca cgccgcgccg ccgcacttcg
     7021 cgcccaccag gaagccctgt tgaaagagaa acaggaacgc caggcacgcc gcgaagccat
     7081 gaaacaacag gcagaacaac aggcaaaagc cgcacaggaa gccaaaaccg gcagacagcg
     7141 tcccgccaaa cctgccgaga aacagcaggc agccgcgcca gccgtcgaaa ataaacctgt
     7201 caatccggca aaagcgaaaa aagaagaccg ccgcaaccgc gatgacgaag gtcaaggccg
     7261 caacgccaaa ggcaaaggcg gaaaaggcgg acgcgaccgc aacaatgcac gcaatggcga
     7321 cgacgagcgc gtacgcggcg gtaaaaaagg caaaaaactc aaactcgagc cgaaccaaca
     7381 cgccttccaa gcaccgaccg aacctgtcgt acacgaagtt ttggtgcctg aaaccattac
     7441 cgttgccgac ttggcacaca aaatggcagt caaaggcgtg gaagtggtca aagccctgat
     7501 gaagatgggc atgatggtta ccatcaacca atccatcgac caagacaccg ccctgattgt
     7561 cgtcgaagaa ctcggacaca tcggcaaacc cgccgccgcc gacgatcccg aagccttctt
     7621 ggacgagggc gcggaagcgg tggaagccga agcattgccg cgtccgcccg tcgttaccgt
     7681 catgggccac gtcgaccacg gcaaaacctc gctgctggac tacatccgcc gtaccaaagt
     7741 ggtacagggc gaagcgggcg gcattaccca acacatcggt gcataccacg ttgaaacccc
     7801 acgcggcgtg attaccttct tggatactcc gggtcacgaa gcgtttaccg ccatgcgtgc
     7861 gcgcggtgcg aaagcaaccg acatcgtgat tcttgtggtc gccgccgacg acggtgtgat
     7921 gccgcaaacc atcgaagcca ttgcacacgc caaagcggca ggcgttccga ttgtggttgc
     7981 cgtcaataaa atcgataaag acactgccaa ccccgaacgt atccgccaag aactgaccca
     8041 acacgaagtc atccccgacg attggggcgg cacggttcaa ttcatcgacg tttctgcgaa
     8101 aaaaggaacg aacatcgacg cgctgctcga agccgtattg ctcgaagccg aagtattgga
     8161 actgaccgca cctgtcgatg cgcccgccaa aggcatcatc gttgaggcgc gcttagacaa
     8221 agggcgcggc gcggttgcca cattgctggt tcaaagcggt acgctgaaaa aaggcgatat
     8281 gctgctggcc ggtaccgcat tcggcaaaat ccgcgcgatg gtcgatgaaa acggcaaatc
     8341 cattaccgaa gccggcccgt ccatcccggt cgaaatcctc ggcttgtccg acgtaccgaa
     8401 tgcgggtgaa gacgcgatgg tattggcgga cgagaaaaaa gcgcgcgaaa tcgccctctt
     8461 ccgccaaggc aaataccgcg acgtgcgcct tgccaaacag caggcggcga agctggaaaa
     8521 tatgttcaac aatatgggcg aaacccaggc ccaatctttg tcggtcatca tcaaggcaga
     8581 cgttcagggt tcttacgagg ctttggcggg cagcctgaaa aaactgtcca ccgacgaggt
     8641 gaaagtgaac gtattacaca gcggcgtggg cggcattacc gaatcggatg tcaaccttgc
     8701 catcgcgtcc ggggcgttca ttatcggctt taacgtgcgt gcagatgcct cttcgcgcaa
     8761 acttgccgaa aatgaaaatg tggaaatccg ctactacaac atcatctatg atgccatcga
     8821 cgacgtgaaa gccgccatga gcggtatgct ttccccggaa gagaaagaac aggttaccgg
     8881 tacggtcgaa atccgtcagg tcatctccgt ttccaaagtc ggcaatattg caggctgtat
     8941 ggttaccgat ggtgtggtca aacgcgattc ccatgtccgc ctcatccgca acaacgtggt
     9001 tatccatacc ggcgagctgg cttcgttgaa acgctataaa gacgacgtaa aagaagtccg
     9061 catgggcttt gagtgcggtc tgatgctcaa aggctacaac gagattatgg aaggcgacca
     9121 actggaatgc ttcgacatcg tcgaagttgc ccgcagcctg taattccttt gcaaataaaa
     9181 tgccgtctga agccttcaga cggcatcgga aaacgggttc tgtatcatac agaacccgtt
     9241 ttttgtcgca aatcggcttc agcgaccctc ctgccttatc ccgatttgaa tctgacttgc
     9301 catacaaaca ggcttcagac ggcattatag tgaattaaat ttaaaccggt acagcgttgg
     9361 ctcgccttgc cgtactattt gtactgtctg cggctcgccg ccttgtcctg atttaaattt
     9421 aattcactat attttcccga caggcggatt tgaagtttct ccacatatcc ggcgcgttcg
     9481 gtacggctgg tttcggggcg gtacgtattg agcgacgacc atttccaatg actgcgggct
     9541 ttgttgagtt cgggcgggag tctggcggcg tcccacgggg ctttgcggct gtgcagctcg
     9601 atatccgact gtgccgcgtg tccgcgcgtt tgcaggacgt ggagcaaatc gagggcgcgg
     9661 gcggcgagca gggtcaaggt ttcagggtcg gtgtgcaagg tttggcggcc ggagagtttg
     9721 tcggaaatgg tgcgggtatt gggcaggatg ccgcccaaaa agccgccgat tgccgtaccc
     9781 aatccgagcg agccgccgag tgtggcgatg tccagcccca agccgatgag cgcgccggtt
     9841 gccgcgcccg tgccggtgcg gatgccgtat tgtttgagca attcgctgtc gaacgggtct
     9901 tggcggaagg cttgcggcat ccagtcgccg ccgtcgattt cgctgtggta gaaacggtag
     9961 agggcaaaca gccgctgctg catctgccgt tcgagttggc gtatttccgc ctgcatggtt
    10021 tgcagcacgg tggcggtatc ctcgttttcg tccacttcct gcctgaaggc ggcggcatcg
    10081 agcaggaaat cggcgatttc gcggcgcgct tcgccgtcca gccgctgcca ttcgcgccgg
    10141 cgcatggctg tcaggcggtc aagtgtgctg cgttcgggca acatggtggc gaggttttcc
    10201 cacaggcgta gttcgccttc aaaatcaaag gcgacggtgt cgaaccctgc gaaaacgtgc
    10261 aggtttctcc tcgccagcat ggttgtccac gattcgggaa gctgtccgcc ggtaaagttg
    10321 aacacgggca taaccggttt ggcacaccat gaaaggatgg tcaattcatc ccgatatttg
    10381 tcgaggacgg gttcgcgcgc gtcgatgacg tacattgcca tatcgctttg caagacttgc
    10441 cgtaagactt tggcttcctg attgaaatca tggtgcgcac cgtggctgcc gagaaactgt
    10501 tgcagccgct cgatgccgtc tgaacgcgta tccgtatggg tttccagcca ttccaaaaca
    10561 ccgcccgcgt cttcgagtcc gggcgtgtca tacaggaaaa ccagcatgtc cgcgccgtcg
    10621 ctgatggcgg cttcttcgac atggcgcgta gtggacgggg catttttgac ttcgccgaag
    10681 ccgctgtcgc gcaatagggt gcgcaggagc gaggttttgc cggtattggt atgcccgacg
    10741 acggcgaggg aaaggggttg tttgttcatg atgtttttga agaatggatt ttcagacggt
    10801 cttttttcag aatggcggct taacagaaca tttcaagtga gtttattggt ctttcaaacg
    10861 cccttcctgc gccgccctgt caggctcaag ccacgccgcg ccgcattcgg ccagcgcgtt
    10921 acgccaatgt cccagctttt ccgaaaggtc gtctgaaagc ccctgttccg ccaaaagctg
    10981 caccaccgcg ccgccctgcg ccgcttccga aagtcggaca atctgccgca gcacgccccg
    11041 gtccggcaca gtttgggcgc gcacgccgat aagcagttgc gccggtttct gcttcagctc
    11101 tgtctccagc gcggcaacct gttcccgatt ggtggcaacg cccttatcca gccattcctg
    11161 tgccagcctg ccctcgaacc acgcgccgtc ctgccattcg gtttccagca tgaccgccca
    11221 tttcggcgca tcgttcaaga cgattttcgg cgaaacggcg gacacggttt cccgacgcgt
    11281 atccgcatcg acgatttgcg tctgccagcg gcggatgacc gcctgataat agggcttttc
    11341 caaatccaag ccgtttttgc ttgttttcaa aaggatttta cacaccaccc aagccaagaa
    11401 gcgcggcagg atgccgtagc aggcgatact gccgaccagc agccccgacc aagcctgcgc
    11461 atcggcaata ttgccgttca gacgaccttc aactaccgcc cgcgcatcgg ggacagggaa
    11521 accgagtttt gacggcagcc atgccaacat ttccaccagc cgtaccgaag cggcattgct
    11581 caacagcgtg ctttcccagt tgaacgtata ttgccgcacc aaaagcagca gcaataccga
    11641 caccagcatt ccgagcagcg tgcagagcca caggctgtgc gacgttgcgc ctattttcca
    11701 acgtaccgaa ggttgccgcc actcgtcggc atacagccgc aacaccgcct gatttacagg
    11761 gtctctgccc cgaaaccacg tcgccggact gctgaaaaaa cgccccactt tcacgcgcag
    11821 gaacaacgtt gccaaccata ctgccaacat aagcgtattc atgcccaaca cgcccgccaa
    11881 aaccaaaaag aaattcagcc cctgattgtc cattagaaga taagtgcttg aaaaaccggt
    11941 aaaaaatgca aacgtcgctg ctgccaccca caaccagaac gaccccgcac gcacacgttc
    12001 caacgtctcc cgcagcatac ggttcctgtc aatcatctcc gcccgacgga tgattttttc
    12061 ctccgtactg ccgtccacgc ggcgcaaagc ctccgtcgcc tgcacgggat cgccgctgaa
    12121 aataaagccg ccttcttcca aaatacggac cagctcaacc agttttcggg atggattcaa
    12181 cataaaatgc cgtctgaaaa taaaaaacag attttaacac acgcattttc aagaatattc
    12241 acagtgtagg caaagagtaa atcccacaca gaggcaaaag tatcggcgta aactgactgc
    12301 ctctactttc ccgaaagatt gtgcgatgta tacaggcgaa cgcttcaata cttacagcca
    12361 tttgagcggt ttgattctgg cggcggcagg tttggtgctg atgctgctga aaaccatagg
    12421 acacggggac ggctaccgta tcttcagcgt atcggtttac ggcatcagcc ttcttctgct
    12481 ctatttgagt tcctcgctgt accacggaat tgcagccgga aaactgaaaa gcattttgaa
    12541 aaaaaccgac cactgcatga tttatgtcct gattgccgga agctacacac cgtttgcact
    12601 ggtttctttg agaaacgggc cgggctggac ggtattttca ctgtcctggc tgctggcggc
    12661 tgcaggaatc gcacaagaac tcaccatcgg acggaaaagc gaaaaacgtc tgctgtctat
    12721 tgtgatttat gtcgtcatgg gttggatggt cttggcggta atgaaatccc tgacagcctc
    12781 actcccgtcg gcaggactgg cttggctggc ggcaggcggt atgctgtaca gcgtcggcat
    12841 ttactggttt gtaaacgatg aaaaaatccg acacgggcac ggaatctggc atctgttcgt
    12901 attgggcggc agcatcaccc aatttgtcag tgtgtacggt tatgtaatct gaatgccgtc
    12961 tgaaaaacaa aacctcccga tcctgaagat tgggaggttt tctgtttgcc ggacatcagc
    13021 ccttgtcgtg gaactcgtgg aattcatacg gataagacaa atcccgaccc gctttttttt
    13081 gtgccaaata atcatcataa atggcacgga tttccttacg cagcaagaac agggcaatca
    13141 ggttggggat aaccataaaa ccgttgaaca tatccgacag gctccaaacc aaatcgactt
    13201 tgccgagcgt acccaaaaca atggcaagca gaaccaatgc gcgatagatg cccaagtgtc
    13261 ttcccctgaa aagaaaacgg atgttggact cgccgaaata ataccagccg ataatggtcg
    13321 taaaggcgaa gaaggtcaga cacacggcaa gcaattgcga accgaagccc gggaatgcct
    13381 tgttaaaggc aaattgagta accgccgcac cctgttcgcc cgaaaggttg gcatcggtca
    13441 gcaggataat cagcgcagtt gccgtacata ccaaaatcgt atcgataaac acaccgacaa
    13501 atgccgccat accttgctgc accggatgct tcacatccgc agtcgcgtgg gcgtgcggag
    13561 tcgaacccat ccccgcctca ttggaaaaca gaccgcgcgc cacgccgaaa cgtatcgctt
    13621 cgcgcatacc gatacccgcc gcaccgccca aaacggcttc gggattgaag gcggcggtaa
    13681 agatgtggtt gaacatcggc acaatatggt cggaaaattc aaacaggata acgacggcgc
    13741 acaaaatata aacaaccgcc ataaacggca cgacaaactg ggcaatattg gcaatacggt
    13801 tcacgccgcc aatcacaacc atgcccgcaa ggacggcaag cacaataccg actgccaaag
    13861 aaggcacatc aaatgcaatg gtaacggcag aagcaatgga gtttgcctgt gtcgcattac
    13921 cgataaagcc caatgcgata atcaacgcaa tggaaaagaa accggacaaa aaacgcgccg
    13981 cgcccctgcc gattttcgga gtcagaccgt gggtgatgta gaacgccggc ccaccgatgt
    14041 atttgccgtg gctgacgacg cggtatttct gcgccagcag tgcctccgca aaaatcgtgg
    14101 acatccccaa aacggcagaa acccacatcc aaaaaatcgc gcccggcccg cctgcggtga
    14161 tggcggtcgc cacgccggcg acgttgcccg taccgatttg cgcagatatg gcaaccgcca
    14221 acgcctgaaa ctgcgataaa gacttgtcgt ctttatcgcc tttggcaaac aagccgccga
    14281 atacggattt gaatcccgcg cccagcttgg taatctgcgg cgcaccgaga tacagcgtaa
    14341 aaaacaggcc gatacccaaa agcgcgtaaa tcagcaggta gtcccaaaga aaccgattga
    14401 ctgtacccac cagaacagac aatatatttt ccataaaata aaccttatct tacaattaaa
    14461 atgactgcct tccaaaagac attccaataa ggaaacacgg cgagcagacc gtatttgccg
    14521 caacagatgc cttaaattgt caacaatcgg ggagaagctg cgccatcata ccgtaaaata
    14581 tcgttaatta aaacatttct ttatttttaa gcggaaagcg gaggaaatcg ctttcagaca
    14641 gcatagacaa cggcacggca taaaacagga tattttgggt acttgcaact tatgttaaaa
    14701 tgccgaccgt aaaaaatctg acaaaaacag attaattatt tgaaataaga aaggaaattt
    14761 atggcaggcc atagcaagtg ggcaaatatc cagcataaaa aagcccgtca ggatgccaaa
    14821 cgcggcaaaa tctttacccg tttaatcaaa gaaatcaccg ttgcggcgcg tatgggcggc
    14881 ggcgaccccg gttcaaatcc gcgcctgcgc ctggctttgg aaaaagcagc cgaaaacaat
    14941 atgcccaaag acaatgtgca acgtgccatc gacaaaggta cgggcaactt ggaaggcgtg
    15001 gaatacatcg agttgcgcta cgaaggctac ggcatcggcg gcgcggcttt gatggtggac
    15061 tgcctgaccg acaacaaaac ccgcaccgtt gcggacgtac gccacgcgtt taccaaaaac
    15121 ggcggcaact tgggtaccga cggctgcgtg gcgttcaact tcgtgcatca gggctatttg
    15181 gtattcgaac ccggcgttga cgaagacgcg ctgatggaag cggctttgga agccggtgcg
    15241 gaagacgtgg ttaccaacga cgacggttcc atcgaagtca ttaccgcgcc aaacgattgg
    15301 gcgggcgtaa aatccgcttt ggaggcggca ggttacaaat ccgttgacgg cgacgttacg
    15361 atgcgcgccc aaaacgaaac cgaactctcc ggcgacgatg ccgtcaaaat gcaaaaactg
    15421 attgacgcgc tggaagactt ggacgacgtg caagacgttt acacttccgc cgtattgaat
    15481 ttggactgat acgcagcaca gcagacatac aataaaatgc cgtctgaacc tttcagacgg
    15541 cattgattta tttagccctt gcccacgccc accaaaccgt caggacaacc gccagaaaat
    15601 acgccgcgct ccaaacaggc aaagcaaccc ctaacagata atccggttcg gcacaatttc
    15661 cgaacccgcg cacgacaggc tcgaaccaat caaacaaagg ccagcccttc aatcgaaacg
    15721 tccacggcgc gccgcacgaa ggagccgtac ccggcggcag cgactgcagc cacaactgat
    15781 atgccgcaac agaaataccc gtaacggccg gaatactgat aaagacagca ccgaacaaac
    15841 cgcccgccct tcttcttggt ctgcacatca ggacaattgc cgtacacaat gcggttgcca
    15901 aaacgcataa ccgctgactg atacacaaaa cgcaaggctc catacccaaa acatactgtg
    15961 ccgccaaaga accggcaaat gcacagaccg aaacggcaaa cagcagccaa acggcttttc
    16021 taaataacgg ggtcattttc tcaacacacc aatcaaaata ccgatatgcc gattttgctg
    16081 gatatatccc gagatggcaa gggacgaatc gacaccggct caggtgcgga cgatttcggt
    16141 tttccccgat atgcataaaa cctgacaagg gacaaaacgg cgatttgccc gcacaatgcc
    16201 cgcaaaaaaa tacatctaga ggatttgaat ttcggcaaat ttagcgaatc aaaagtttat
    16261 ttcaatgaaa ttatatgatt tttttgaata agcggattga caggtttttg aaggaatttg
    16321 ttaccggata gccatcgggc aagttttttg caaaatttga atcggtcggg taaattttca
    16381 aaaaataatt gacagcggat aagaaacggc ggataattct cgccgtcgag ttgcttgatg
    16441 cagcttgatt tttctcctct atttctcctt tgtagacttg gcacacattc aaccggatgt
    16501 gtgcattttt ttatctccgc tttttttgaa atttaattac ttttattgtt tgaaattcca
    16561 ttctttaaga aatttaacaa gaaaaacgct tgctttttga ccggaaaagc tgcataattc
    16621 attccgttag ctggttcgag tagtcagtta atagtttctc ctctatttct cctttgtaga
    16681 cttggcacac attcaaccgg atgtgtgcat ttttttatct gaagcaacaa gcctctgtgc
    16741 gtgatgttgt tatgtttcat ttaggtgtca aaccgcatag ccagcctgaa atattcaatc
    16801 caaatccaaa accggatttt ctttgacctc ctccatcaca acataactcc tactctccga
    16861 agcggcaggc agttgcaata ggatattgcc tagcatatcc cgataggcag acatatcggg
    16921 caaacgtact ttaatcagat agtcgtattc gcccgacacc aagtggcatt ccataatttg
    16981 cggaattttc agcacttctt ttttgaaatc ttcgaaaata ttgcccgatt tggattgcag
    17041 cttcagctcg acaaaaacca atagcggttt gcccaacaga tggggattga ggtgggcgtg
    17101 atagccggaa atataatgtt cccgctccaa acggcgcacc ctctctgtaa cgggcgtggt
    17161 ggacaagcct accttctcgg caagctccgt catcgggatg cgggcattct gttggaggat
    17221 cttaagaatg cggaaatcga ttttatctag ttctttcatt tggattgcct tgtatttatt
    17281 attgatttta acaaatagag tatatagtgg attaacaaaa accagtacgg cgttgcctcg
    17341 ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
    17401 cactatatat ttgggaaagc gattatatca ggaaaagcaa accgccttcc tacctaaaaa
    17461 ctgctgcttc ggcttgaaga tacaagggtc tttaatattt taaaagcctt gccgttggat
    17521 tataattccc taattgattt cttaattttg ctaataaaca cttgtttggt aaggaatgaa
    17581 tttatgcgcc ctttgaacgt gcagatcagg ttgggcaacc ttaggcacaa ttatcggatt
    17641 ttgaaggaaa tgcacggagg caaactgttg gcggtagtga aggccgacgc atacggacac
    17701 ggtgcggtca gatgtgcttt cgcgctggca gacttggcag acggctttgc cgtggcgaca
    17761 atcgacgagg gaatcaggct gcgggagagt ggcattaccc atccgattgt ccttttggaa
    17821 ggcgtatttg aagcatcaga gtacgaagcg gtcgaacaat actcgctctg gccggcagtc
    17881 ggaaaccaat ggcaacttga ggctttgctt tcccgccatt ggaaaaaacc tgtcaaggtc
    17941 tggttgaaaa tggattcggg aatgcaccgt accggatttt tccctcatga ttacgcttcg
    18001 gcatatgcgg cattgaagca gtcggaatat gtggacagta ttgtcaagtt ctcgcatttc
    18061 tcctgtgcgg acgaacccga aagcggtatg acggaaatac agatggaagc attcgatttg
    18121 ggtacggaag ggctggaagg cgaagaaagc cttgccaatt cggcggctat tttgaatgtt
    18181 cccgaagcac gcagggattg ggggcgcgcg ggcttggcgt tatacggcat ttccccgttc
    18241 ggaggcagcg atgacaggct gaaacccgtg atgaggcttt caacccgtat ttttggcgaa
    18301 cgcgttttac agccgcactc ccctatcggt tatggcgcaa cattttatac cagcaaatct
    18361 acgcgcgtcg gtctgattgc ctgcggttat gcggacggtt atccgcgccg cgccccaagc
    18421 aattcccccg tcgccgtcga cggtaaattg acccgggtca tcggcagggt ctctatggat
    18481 atgatgacca tcgagctgga cgcttcgcaa gaaggtttgg gacacgaggt cgaactgtgg
    18541 ggcgatacgg tcaacatcaa tactgttgcc gaagcggccg gaaccatccc ttacgaattg
    18601 atgtgcaata tcaaacgtgc aaaattcact tatatcgagt aatcaagtcc aaacaaaaat
    18661 gccgtctgaa gcctttcaga cggcataaat gcgttttgac aaaatcagta tagtggatta
    18721 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatcgtacgg caaggcgagg
    18781 caacgccgta ctggtttaaa tttaatccat tataaaagac attgccaaat agaaaatact
    18841 gataatgccc acaatctgaa accccatcct tcccgcaaaa acggaaaatc aaaatcagaa
    18901 acctaaaatt tcgtcattcc cgcgcaggcg ggaatccggt tttttgagtt tccgtcattt
    18961 ctgataaatt ctcgtcgttt ttcatttcta gattcccgcc tgcgcgggaa tgacaaaatt
    19021 aaagtttcag aatttatttg aacaacaaac tagattcccg ctttcgcggg gatgccggcg
    19081 gataggtttt gttgtttcgg ataaattcct gttgtttttt ggtttccaga ttcccacctt
    19141 taaaagaatg acgtaagaaa gcagaaataa ggacacaggg attttttata gtggattaac
    19201 aaaaatcagg acaaggcgac gaagccgcag acagtacaga tagtacggca aggcgaggca
    19261 acgctgtact ggtttaaatt taggggctgt cctagataac taggataaac tcggttttac
    19321 taattgtttt aa
//
LOCUS       DAXHTY010000035        17531 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXHTY010000035 DAXHTY010000000
VERSION     DAXHTY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17531
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.035"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="ACFNMK_001002"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="ACFNMK_001002"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6364290.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDVYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIFDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMINTKTVAVEPKEDGVYVTFEGTNAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1965..3572)
                     /gene="aceF"
                     /locus_tag="ACFNMK_001003"
     CDS             complement(1965..3572)
                     /gene="aceF"
                     /locus_tag="ACFNMK_001003"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC6364291.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGTAIIEVETAGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQ
                     GGAAKPAAAGASLGSSLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3722..6385)
                     /gene="aceE"
                     /locus_tag="ACFNMK_001004"
     CDS             complement(3722..6385)
                     /gene="aceE"
                     /locus_tag="ACFNMK_001004"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC6364292.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGLEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7080..8792
                     /locus_tag="ACFNMK_001005"
     CDS             7080..8792
                     /locus_tag="ACFNMK_001005"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC6364293.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHTDYASLQTTYDAMYDAYCRIFTRLGLEFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERTAAQAELTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVESENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAIAIGEIVARVTA
                     SLNA"
     gene            8855..9511
                     /locus_tag="ACFNMK_001006"
     CDS             8855..9511
                     /locus_tag="ACFNMK_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC6364294.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGQDADGMGEGEDILGCVAGYGVGLDLTA
                     RDIQCHLKAQGLPWLKAKGFRHSACVSDFAAADRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9521..9976)
                     /gene="ruvX"
                     /locus_tag="ACFNMK_001007"
     CDS             complement(9521..9976)
                     /gene="ruvX"
                     /locus_tag="ACFNMK_001007"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC6364295.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTEHEMTHLSRKFGRRLHGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGRKG"
     gene            complement(9969..10517)
                     /locus_tag="ACFNMK_001008"
     CDS             complement(9969..10517)
                     /locus_tag="ACFNMK_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC6364296.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10519..11079)
                     /locus_tag="ACFNMK_001009"
     CDS             complement(10519..11079)
                     /locus_tag="ACFNMK_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC6364297.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11407..13212
                     /locus_tag="ACFNMK_001010"
     CDS             11407..13212
                     /locus_tag="ACFNMK_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6364298.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKVLLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIASGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13339..14823
                     /locus_tag="ACFNMK_001011"
     CDS             13339..14823
                     /locus_tag="ACFNMK_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC6364299.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAVPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            15080..17170
                     /gene="uvrB"
                     /locus_tag="ACFNMK_001012"
     CDS             15080..17170
                     /gene="uvrB"
                     /locus_tag="ACFNMK_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC6364300.1"
                     /translation="MPSERRFRPIPTTHFVRKRITGKIMEVIQYPNSPFKLHQPFPPA
                     GDQPTAIAGLLEGLSDGLAYQTLLGVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQ
                     LYAEMREFFPENAVEYFVSYYDYYQPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNL
                     MTRDDVIIVATVSAIYGIGDPTEYQQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDF
                     KRGSFRVRGDVIDVYPAESSENALRISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPS
                     SHYVTPRDTVLRACESIKEELRERIDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCK
                     GIENYSRHFSGKKEGEPPPTLMDYLPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLV
                     DYGFRLPSARDNRPLKFHEFEKVMPQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDP
                     QIIIRPVATQVDDLMSEINDRIQKGERVLVTTLTKRMAEQLTDYYSELGIKGRYLHSD
                     IDTVERVEIIRDLRLGLFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQ
                     TIGRAARNVNGVAILYADKITDSMKAAIDETERRREKQIKFNEEHGIVPQQIKKQVKD
                     IIDGVYHEEDSGKGRLKGKNKVKVGEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAV
                     LRDRIRGIKENLLF"
     gene            complement(17422..>17531)
                     /locus_tag="ACFNMK_001013"
     CDS             complement(17422..>17531)
                     /locus_tag="ACFNMK_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFC6364301.1"
                     /translation="EIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH"
BASE COUNT         4273 a         4435 c         4801 g         4022 t
ORIGIN      
        1 ccggaaaaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacgacc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggaggctt
      421 tggcggacaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgcccg
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcggcg gcaacgtggc
      541 cttcgtgaac ggctttgtgc gccaacatcg gctgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggtaacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttcg gtgcgttcgt gccttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gatcatgatg ttgtcaaaac
      841 gatattcgtt ttgtttttgc catactttca ccaagtcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaacaaca tccaggcgag aacccagcgt gctgtaaacc gtacccattt
      961 cgagaccgat aataccgcca ccgatgatca gcagtttgcc cggcacttct ttcagcgcca
     1021 aagcgccgct ggagtcgaaa attcgaggat cttcagggat gaaaggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagggg
     1141 ctgcctgttc gtacacatcg ccggcagtca gcgacacttc caagtggtgc ggatctaaga
     1201 attgcccgtc gccttggata acgtccactt tacggctttt cgccatacct gccaaaccgc
     1261 ccgtgaggcg ggaaactacg ccgtctttgt aggcgcgaag catatcgatg tcgagttccg
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacaa ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaagttt
     1441 tgtaacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg accgccaccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 ccgcttcggc tttaggagcg gcagccgtgc cttcagcttc aacgacaaca atcaaaccgc
     1681 cttcggagat tttgtcgccg actttgactt tcacttcttt gaccacgccc gcaacttcgg
     1741 caggcacgtc catcgtcgct ttgtcggttt ccaaggtaat cagggtgtcg tcaacggcga
     1801 tggtgtcgcc cacgtttact tcaaccgcga taatatctac attttcgtgt ccgccaatgt
     1861 cgggcacttt caattcaact aagctcatgt ctcatctttc tgaatgatgt tcaaacttcc
     1921 ttttcagacg gcacagaccg ctatgccgtc tgaagccgat cgggttacag ggtaatgcgg
     1981 cggaagtctt tcaacagctt cgccaagaat acggtgaagc gcataccggc cgcaccgtcg
     2041 atgacgcggt ggtcgaagga caggctcaac gggcacatca ggcgcggggc aaactctttg
     2101 ccgttccaaa ccggtttgat ttgggatttg cacacgccca agatggcgac ttcgggagcg
     2161 ttcacaatcg gcgtgaaacc tgtgccgccg atgccgccca agctggaaat ggtaaagcac
     2221 gcgccttgca tttcttgcgg tttgagcttg ccttcgcggg cttttttaga caattcggtc
     2281 agctcttggc tgatttgttt caagcctttt tgatccacgt ctttgatgac gggaacaacc
     2341 aagccgttcg gcgtatcggc tgcgaaaccg atgttgaagt agtttttcag caccaggttg
     2401 tcgccgtcca aagaagcgtt gaattcgggg aaggctttca gcgcggaaac ggaggctttg
     2461 atgatgaacg ccagaggaga cagtttcacg ccttcgcgtt cccattcttt gttcagttgt
     2521 ttgcggaatt cttccagctc ggtcatatcc gcttcttcgt gtacggtaac gtgcggaatc
     2581 acaacccagt tgcgagacag gttttgaccg gagattttct taatgcggga caattcttta
     2641 acttcgacat tgccgaattt ggagaagtcc actttaggcc acggcagcaa gtccagactg
     2701 ctgcccaaag atgcgccggc tgcggcaggt tttgccgcgc caccctgcat tacggatttc
     2761 acaaaggctt tgatgtcgtc gcccacgata cggcctttca agccggtacc tttgacttgg
     2821 cccaaatcca cgcccaattc gcgcgccagt ttgcgtgcgg aagggccggc gtgtgctttg
     2881 gcgaaagcgg cctcgtcgat tttggcagcg gcaggtgcag aaggtgcagg cgcggcggcg
     2941 ggtgcggcgg cagcagtcgg aggcggagcg gcagccggtg cggcagcttg agcggcttga
     3001 gcaggggctg ctgcggcaga gccggcggtt tctacttcga taatggcagt accttcggac
     3061 actttgtcgc cgacttttaa gaatacggct ttaacgacac cggcagcggt acaaggtacg
     3121 tccatcgttg ctttgtcggt ttccaaagta atcagcgtgt cgtcttcggc aacggtatcg
     3181 ccgactttga tctcgacggc gattacatcc acatcggtat ggccgccgat gtcgggaacg
     3241 gctacttgaa cggttgcacc gcctgcggca acgggtgcgg cagcaggtgc aggttgtgct
     3301 tcggcagcag caggagcggc ttcggcagcg gcggcaccgg tttcaacggt caaaattacg
     3361 ccgccttcgg agattttgtc gccgactttg actttaactt ctttcacgac accggccgca
     3421 tcggcaggca catccatcgt ggctttgtcg gtttccaaag taatcagggt gtcgtcaacg
     3481 gcgatggtgt cgcccgcttt aacttctacg gcgatgatgt cgacgttttc gtgaccgccg
     3541 atatcgggga ctttgatttc tacgatactc atttgagttc ctttaatctt tttcaattgg
     3601 atgccgtctg aaagctgctt caggcggcat cggtacggtt tgtctgctca aaccattcgg
     3661 gcattcggtt ttcagacggc ctgatgtcgg aatggggcaa acaggccgtc tgaaacatca
     3721 atcagcgttt ccagctagga gctgaatcgg ctttgatgcc gtatttctca atggcttgtt
     3781 gaacggtttc tttgctgact ttgccttgtt ccgccaatgc ggccaatgcg gccacggcaa
     3841 cgttgtagcg atccacttca aagaagcggc gcaggttggc gcgactgtcg gaacggccga
     3901 aaccgtcagt gcccaagacg tggtagtcgt tcgggatgta ggcgcggata cggtcagcat
     3961 agctgcggat atagtcggta gcggcaataa ccggaccgtc atgaccttgc agttgggaag
     4021 taacgaaagg tactttttcg gcttccagcg gatgcaggcg gttgaagcgt tctacctcga
     4081 cagcgtcgcg gtgcagcagg ttgaaggatg ggcaagacca aatgtctgct tccaagccga
     4141 agtcggcttt cagcagctcg gcaccggcaa tcacttcttg caaaatcgta ccggaaccca
     4201 tcagttggac tttcttgtcg cctttgccgc cggctttcag caggtacata cctttcaaga
     4261 tgtcttgttc cgcaccttcg ggcatatccg gatgggtgta gttctcgttc atcagggtga
     4321 tgtagtagaa cacgtcttca ttattggcat acatacggcg cagaccgtct tgtacgatga
     4381 cggcgacttc gtattggaaa gtcgggtcat aagatacgca gttcggaatc aggtcggcct
     4441 ggatgtggct gtggccgtct tcgtgttgca ggccttcgcc gttcagtgtc gtacggccgg
     4501 cagtaccgcc cagcaggaag ccgcgcgcgt gcatatcgcc cgccgcccaa gccaagtcgc
     4561 cgatacgttg gaaaccgaac atagaatagt aaatgtagaa cggaatcatg gcgaagttgc
     4621 tgttggcgta gctggttgca gccgcaatcc agtcggccat cgcgcccggt tcgttaatac
     4681 cttcttgcaa gatttgaccg tcaacggatt ctttatagaa catcagttgg tctttgtctt
     4741 gaggggtata ttgctgacct ttcggattcc aaataccgta ttggcggaac ataccttcca
     4801 tgccgaaagt acggctttcg tcgggaacga taggtacgac gcgtttgccg atttttttgt
     4861 ctttcagtaa agtggacagg atgcggacga atgccatcgt ggtcgagaac tcgcgttcac
     4921 cgctggattt gagttgtgcg tcgaatgctg acagctcggg cacttccaat acttcctgcg
     4981 tcggtttgcg ttgcggcagg tagccgccca aagcatcgcg gcgtgcgtgc aggtatttgt
     5041 attcttccgt atcgggggca aaagtcaggt aaggcagatc gccgctttcg atttgttcgt
     5101 cggtaaccgg aatgtcaaag cggtcgcgga attgtttcag ggacgctttg tccatttttt
     5161 tggcttggtg ggcaacgttc tgaccttcgc cggatgcgcc cataccgtaa cctttaatgg
     5221 ttttcgccaa gatgacggta ggtttgccgt cggcatggtt cgctgcgcgg tcgtaggcgt
     5281 tgtacacttt ttgcgggtcg tggccgccgc ggttcaatgc ccagagttgc tcatcggtca
     5341 tatcggcaac caatgctttc agttcgggcg tattgaagaa gtgttcgcgc acatacgcgc
     5401 cgtctttgga tttgtaagtt tggtagtcgc cgtccaaaca ttcttccata cgttggcgca
     5461 ggataccgtc tttgtctttc gccaagaggc ggtcccagcg gcggccccaa atgactttga
     5521 cgacattcca gccggcgccg gcaaagttgc cttccaattc ttggatgatt ttgccgttgc
     5581 cgcgtaccgg accgtccaag cgttgcagat tgcagttgat gacgaaaatc aggttgtcca
     5641 agccttcgcg tgcagccagt gcgattgcac cttgagattc gggttcgtcc atttcgccgt
     5701 cgccgcagaa acaccatact ttacggcctt tggtttttgc caaaccacgc gattccaagt
     5761 atttcaggaa acgcgcctga taaatcgcca tgatgggtcc caagcccatg gatacggtcg
     5821 ggaactgcca aaagtcgggc aagaggtggg ggtgcggata ggaaggcaga ccgtgtccgt
     5881 ccacttcttg gcggaagttg ttcagctggt cttcggtcag acggccctcg acgaatgcgc
     5941 gtgcatagat gcccggggcg acgtgacctt ggaagaagac caaatcgcct tcttcgcctt
     6001 cgcctttggc tttccaaaag tggttgaaac cgacttcgta catggtggcg gcagattgga
     6061 aagatgcgat gtgcccaccc agttccaaat ctttcttgcc ggcgcgcaat acgatggcgg
     6121 cggcgttcca gcgcacgaat gcgcggatgc ggtgttcgat gttttggtcg cccggaatgc
     6181 ctttttcgtt ttcaaccgaa acggtattca aatacggggt ggtcgtgccg tgtggcatac
     6241 gtacgccctt gtcgcggcag tatttgacca ggttttccaa gaggtattgc gcgcgttcgc
     6301 cgccttcata ttcgaggacg gagcttaacg cgtccagcca ctcttgggtt tcgatagggt
     6361 caacatcgtg taattgggtg gacatagtat ctatccttta tgttgagtgt ttaatcggca
     6421 gggagggtgt agccaacccc tttattttcg tttgtgaaaa ataataagca aaaacgaaaa
     6481 tttctaaatc aaaccgaaaa aaaataattt aagtttaatg ttttcaaaaa attataaaag
     6541 ttttggaaac aagggaaaac ttgaaaaaaa tgttcgatct gctccacacg aaatcctatc
     6601 agcaatcggg tctgctggca aataaccatg cctgtaaaag caggttttat actgaagttg
     6661 gcgtttggta aggtcaagtc tttttgattt cgaaatattc tgtttgattt tggcgatatt
     6721 ttttcggaat tgtggtatat cgaatcatta tgtctctgtc ggcaattttc ctattgtaga
     6781 aaatctatac acaagctttg tggataaccg tgttgataag ccacggcttg tgttaaaaaa
     6841 gtcttacgca acaaatgtac gcttattttg attaaaaaat aggcacattt aaatctataa
     6901 aaatcatgtg cttatgttca atatgatgtt tctggtcatg tttttgccgt ctgttatggg
     6961 atttgtcccg cgcttggggc agggggtgaa ttttggaggt tggtgcaaaa cctgcttttt
     7021 tcgcgttaca atctgcaccc gatttacttt tgaatttgcc tgtttggaaa aatactttta
     7081 tgaaagccag ccaattcttt atctctactt taaaagaagc ccctgccgaa gccgcgcttg
     7141 ccagccacaa gctgatgatt cgcgccggtc tgattaaagc caacgcgtcc ggcctttata
     7201 cttggatgcc gatggggctg cgcgtgttac gcaaagtcga aaacgtcgtg cgcgaggaaa
     7261 tggcgcgcgc gggcagtgtg gagctgctga tgccggtggt gcagcctgcc gagctgtggc
     7321 aggaatccgg ccgctgggag ttttacggta aagaactgct gcgcctgaaa gaccgccacg
     7381 accgcgattt ctgcatgggc ccgacctgcg aggaagtcat cgccgacatc gtgcgtaaag
     7441 agatcaacag ctacaaacaa ctgccgaaaa acttttacca catccaaacc aaattccgtg
     7501 acgaagtgcg cccgcgcttc ggtgtgatgc gtgcgcgcga gtttgtcatg aaagacgctt
     7561 attccttcca taccgactac gcctcgcttc agacgaccta tgatgccatg tatgacgctt
     7621 actgccgtat cttcacccgt ctgggcttgg aattccgccc cgttgccgca gataccggca
     7681 gcatcggcgg tacgggttcg cacgagtttc aagtgttggc ggaaagcggc gaagatgtta
     7741 ttgcgtacag cgacacttcc gattacgccg ccaatatcga gttggccccg accttgccgc
     7801 ttaaaggcga acgcaccgct gctcaggccg agttgaccaa agtacataca ccaaatgtca
     7861 aaaccattga gtctttggtt gaattcctga atattccggt tgaacaaacc ctcaaatcca
     7921 tcgtggtcga aagcgaaaac gaaggcgaaa tcgtcttatt actgttgcgc ggcgaccatg
     7981 agttcaacga catcaaagcc gaaaaactcg cgggcgtgaa atcgccattg acgatggcaa
     8041 gtcctgccgc gattgtcgaa cagttcggcg caaacggcgg ctcgctcggc cctgtcggct
     8101 tcacaggcaa agtctatgcc gatttcgcta ccgaaaaagg cgcggactgg gttatcggcg
     8161 caaacgaaga cgactaccac tataccgggt tcaatttcgg ccgagacgct gccgagcctg
     8221 agtttgtcga tttgcgcaac gtcgtcgaag gcgacgaaag ccccgacggg caaggccgtc
     8281 tgaaactggc gcgcggcatt gaagtcggac acgtcttcca gttgcgtgac aaatatacta
     8341 aagcgatgaa tgtcagcttc ttggacaaca acggcaaatc gcaaattatg gaaatgggct
     8401 gctacggcat cggcatcacc cgcgtcgttg ccgccgccat cgagcagaat aacgacgaaa
     8461 aaggcatcat ctggaccaaa gcaatggcgc cgtttgaagt cgttatcgtg ccgatgaact
     8521 acaaaaaatc agacaccgta cgcgaagccg ccgacaaaat ctatgccgaa ttgctggcgg
     8581 caggcgcgga tgtgctgctg gatgaccgcg acgaacgcgc aggcgtattg ctgaacgatt
     8641 ccgagcttct cggtattccg caccgcatcg tcatcggcga ccgcgccttg aaagaaggca
     8701 atgtcgaata cgccgaacgc cgcgacaacg aagcgcaggc aattgcaatc ggagaaattg
     8761 ttgcacgtgt aacagcttca ttaaatgcgt aagcttggtc gaatgccgtc tgaaaataat
     8821 ttcagacggc atttttactt agggggaaag tgaagtggct tcagtatttt tagaaggaga
     8881 ggcagtcgag gtcggcaata ttttctgtat cggcaggaac tatgccgcac atatcgaaga
     8941 gttaaaaaac gaaatacctt ccgaacctgt ggtgtttatg aagccgtcag gcagcatttt
     9001 gaatagcggc ggtacaatct tgctgcccga gtttagtagg gatgtgcagt ttgagtgcga
     9061 actcgtgttg ctggtcggtc aggatgcgga cggcatgggc gagggtgaag atattttggg
     9121 atgcgtggcg gggtacggcg tgggtttgga tttgaccgcg cgggacatcc aatgccatct
     9181 gaaggcgcag gggctgccct ggctgaaggc aaaagggttc aggcattccg cgtgcgtgtc
     9241 ggattttgcg gcggcagaca gaatcggcaa tcccgagaag gtgttgtttt cattaaaaca
     9301 aaacggcgta ttgaaacagc gcggggatac cggtttgatg atttacccga ttcgggaaat
     9361 tttacacaaa ctggcggcgg attacggttt ggggaaaggg gatttggtgt ttaccggaac
     9421 gccctccggc gtgggcgcaa tcggtgcggg ggacaatttg gcattggagc tggacggctt
     9481 ggtgcgcgcc tctttcacgg tagggtgtta accgcgccgc ttaaccctta cgcccgttga
     9541 aacattccgc cggaccgccc tcgaaaaaac cgtgcaagat ggcttgcgcc gccacttggt
     9601 cgagcaccga tttgcgtttt ttgccgaaga cctgtgcttc cgaaagcagg ctttcggcat
     9661 agacggatga cagccgttcg tccacccaat agacggggag attgaacctg ccgtgcagcc
     9721 tgcgcccgaa cttgcgggac agatgcgtca tttcgtgttc cgtgccgtcg gtatgcacgg
     9781 gcagtccgac gacaaaataa cgcggctgcc attcttgaac cagcttggcg attgccgcga
     9841 acttttcatc gttgctgccg ccggtaacgg tgctcaaagg atgggatagc cctaattccg
     9901 cgtctccttg tgccacgccg atacgcgctt cgccgaagtc gaatgccagt gccgttcctt
     9961 ttggaatttt atgcatggcc ggctcccgaa aacagggcga gcgggtcgat gccgagtttg
    10021 gcaaaggcgg cggcgtaacg gtgttcgtag gggatgtcga acaagatatg ttcgtcggcg
    10081 ggaacagtca gccacgcatt gtcggcaagt tcgcgttcga gctggccttt tccccagctt
    10141 gaatagccta tgctgatcaa ggctttgtca accgcacctt cgcgtgaaat gttttcaagc
    10201 acatcgcggg aagaggtcag cgcgatgccg tctgaaacgc cgatgctgct ttgccagttg
    10261 ccgatcgggg tatgcacgac ataaccgcgc tcgacctgca ccggcccgcc catcatcacg
    10321 ctgtcgtgct gcatccgcat ggggatgttt ttgccggtgg cggaaaaaat catgtccatc
    10381 gtaatcggag agggtttgtt gatggcgatg ccgagtgcgc cgtcttcatc gtgtttgcag
    10441 atatagacga ccgattgtga aaaaaacgcg tcttccatat cgggcatggc gacgagaaag
    10501 tggttggaga ggttcatatc agcgtttgtc gggattttct atcgggttgg aaatcatgca
    10561 ggcgccggca agcaggttgc cggaagtatc ggcttgcacc ccatcggttt gcggtatgcc
    10621 gccgccgaaa caggaaaccg cgcttaagga atttttcggc gagccttgga tgattttatc
    10681 gctgtacacc aaataggcga aggttttgcg tttggggtcg taataacgga caatctgccg
    10741 gctcttgaac gcgaagctcg caccgtgttt gaaaacttct ttcggtttgc gcacggcggt
    10801 ttcgtcaaaa gaaatcgaag atgccgtctg aacgcacgaa accgatgcgt cggacgcgtc
    10861 ctcttccaaa ttgaccattt ccttcaagcc gccttttttt gcatacgaaa tataacaggc
    10921 aaccccttga acgtcgggat cgtcgaatcc ttccacttcg atacggtcgt ttttgcccag
    10981 catgttgaaa acggtacttg cccgtccgat tttatcggtt tcgccgctgc cgcaggcagt
    11041 cagcaggacg gcggcagaca gcagtagcag tctgttcatc aaaatttcct tatcggttga
    11101 atccccgttt tccgtgaagg cggcagggct tgctttgttt ggaggggcgc acgttcccaa
    11161 aatcgggcaa catacggtat gcggcttaat gtgctaccct gtgtgtcgga acacaatatc
    11221 cgttgcggca ttgaaaatat gacgcacaat ataaggctgt ttgagccgat tgcaagtatc
    11281 cgcgcaaaga aggcgcaaga ttgggcaaac ggcattttca gacggcataa ctgacagtat
    11341 aatccaaacg tccggctgct ttcgtgcagc cttcagtatt atccaacttt ttccgaaggt
    11401 cgaaccatgc gctacaaacc ccttctgctt gccctgatgc tcgttttttc cacgcccgcc
    11461 gttgccgccc acgacgcggc acacaaccgt tccgccgaag tgaaaaaaca gacgaagaac
    11521 aaaaaagaac agcccgaagc ggcggaaggc aaaaaagaaa aaggcaaaaa tggcgcagtg
    11581 aaagataaaa aaacaggcgg caaagaggcg gcaaaagagg gcaaagagtc caaaaaaacc
    11641 gccaaaaacc gcaaagaagc agagaaggag gcgacatcca ggcagtctgc gcgcaaagga
    11701 cgcgaagggg ataagaaatc gaaggcggaa cacaaaaagg cacatggcaa gcccgtgtcc
    11761 ggatccaaag aaaaaaacgc aaaaacacag cctgaaaaca aacaaggcaa aaaagaggca
    11821 aaaggacagg gcaatccgcg taagggcggc aaggcggaaa aagacactgt ttctgcaaat
    11881 aaaaaagtcc gttccgacaa gaacggcaaa gcagtgaaac aggacaaaaa atacagggaa
    11941 gagaaaaatg ccaaaaccga ttccgacgaa ttgaaagccg ccgttgccgc tgccaccaat
    12001 gatgtcgaaa acaaaaaagt cctgctcaaa caaagcgaag gaatgctgct tcatgtcagc
    12061 aattccctca agcagcttca ggaagagcgt atccgccaag aacgtatccg ccaagagcgt
    12121 atccgtcagg cgcgcggcaa ccttgcttcc gtcaaccgca aacagcgcga ggcttgggac
    12181 aagttccaaa aactcaatac cgagctgaac cgtttgaaaa cggaagtcgc cgctacgaaa
    12241 gcgcagattt cccgtttcgt atcggggaac tataaaaaca gccagccgaa tgcggttgcc
    12301 ctgttcctga aaaacgccga accgggtcag aaaaaccgct ttttgcgtta tacgcgttat
    12361 gtaaacgcct ccaatcggga agttgtcaag gatttggaaa aacagcagaa ggctttggcg
    12421 gtacaagagc agaaaatcaa caatgagctt gcccgtttga agaaaattca ggcaaacgtg
    12481 caatccctgc tgaaaaaaca gggtgtaacc gatgcggcgg aacagacgga aagccgcaga
    12541 cagaatgcca aaatcgccaa agatgcccga aaactgctgg aacagaaagg gaacgagcag
    12601 cagctgaaca agctcttgag caatttggag aagaaaaagg ccgaacaccg cattcaggat
    12661 gcggaagcaa aaagaaaatt ggctgaagcc agactggcgg cagccgaaaa agccagaaaa
    12721 gaagcggcgc agcagaaggc tgaagcacga cgtgcggaaa tgtccaacct gaccgccgaa
    12781 gacaggaaca tccaagcgcc ttcggttatg ggtatcggca gtgccgacgg tttcagccgc
    12841 atgcaaggac gtttgaaaaa accggttgac ggtgtgccga ccggactttt cgggcagaac
    12901 cggagcggcg gcgatgtttg gaaaggcgtg ttctattcca ctgcaccggc aacggttgaa
    12961 agcattgcgt cgggaacggt aagctatgcg gacgagttgg acggctacgg caaagtggtc
    13021 gtggtcgatc acggcgagaa ctacatcagc atctatgccg gtttgagcga aatttccgtc
    13081 ggcaagggtt atatggtcgc ggcaggaagc aaaatcggct cgagcgggtc gctgccggac
    13141 ggggaagagg ggctttacct gcaaatacgt tatcaaggtc aggtattgaa cccttcgagc
    13201 tggatacgtt gatcggattg ccgaaacggt aggctttatg caaatcgtgc cgatatggtg
    13261 cgctgttccg tgaagtttgc cgtttgccgt acctgtattt tccggttttt ggaaatcaag
    13321 agagaaagtt aaagaagaat gtcgaaacct gtttttaaga aaatcgcact ttatactttg
    13381 ggtgcaatca gcggcgtggc cgtcagtctg gcggtgcagg gttttgccgc cgagaaggac
    13441 aggcgggata acgaagtcct gccggtgcaa tccatccgca caatggcgga ggtttacggt
    13501 caaatcaagg caaactacta tcaggacaaa cccgatgccg atttgtttga aggtgcgatg
    13561 aagggtatgg tggccggttt ggatccgcat tccgaatata tggataaaaa aggttatgcc
    13621 gagataaagg agtccaccag cggcgaattt ggcggcttgg ggatggaaat cgggcaggaa
    13681 gacggatttg tcaaagtggt ttcgccgatt gaggacacgc ctgcggaacg ggcgggggtg
    13741 aaaagcggcg atttcattgt gaaaatcgat aatgtttcga cacgcggcat gacggtcagc
    13801 gaagcggtga agaaaatgcg gggcaagccg ggtacgaaga ttactttgac gctgtcgcgc
    13861 aaaaatgccg acaagccgat agtcgtcaac ctgacccgtg ccattattaa agtgaaaagc
    13921 gtccgccatc acctgatcga acccgattac ggctatatcc gcgtgtcgca gttccaagag
    13981 cggacggtcg aaagcgtcaa taccgccgca aaagagctgg taaaggaaaa taaaggaaaa
    14041 ccgctcaagg ggctggtgtt ggatttgcgc gacgaccccg gcgggctttt gactggcgcg
    14101 gtcggcgtgt cggcggcgtt tctgccgtct gaagcagtcg tcgtcagcac caagggacgc
    14161 gacggcaaag accgcatggt actgaaagcc gttcctgaag attatgtgta cgggatgggc
    14221 ggcgattctt tggcgggcat tcctgccgag ttgaaaacca taccgatgac ggtattggtc
    14281 aattccggtt cggcttccgc gtcggagatt gtcgcaggtg cattgcagga tcataaacgc
    14341 gcggtcatcg tcggtacgca gagcttcggc aaaggttcgg ttcagacttt gattcctttg
    14401 tccaacggca gcgcggtcaa gctgacaacg gcactgtatt atacgccgaa cgaccgttct
    14461 attcaggcgc aggggattgt tcccgatgtc gaagtaaaag ataaggaacg catttttgaa
    14521 agccgcgagg cggatttggt cggacacatc ggcaatcctt tgggcggcga ggatgtgaac
    14581 agtgaaaccc ttgccgtgcc gcttgaaaaa gatgcggata agcccgctgt aaaagaaaaa
    14641 ggtaaaaaga aaaaggacga ggatttgtct tcaaggcgga ttcccaaccc tgccaaagac
    14701 gaccagttgc ggaaagcttt ggatttggtc aagtcgcccg agcagtggca gaagtctttg
    14761 gggctggcgg cgaaaaagcc ggtttcaaat aaagataaga aagataaaaa agataagaag
    14821 taggttttta cgatgccgtc tgaaaggatt tcagacggca tattatgttt gaaaggacag
    14881 gttatgagcg attttttacc ggaatttgaa ccgtgcggca tttatagtgg attaacaaaa
    14941 atcaggacaa ggcgacgaag ccgcagacag tacaaatagt acggcaaggc gaggcaacgc
    15001 cgtactggtt tttgttaatc cactatactt gggtagcgat tgggtttcag acggcataca
    15061 ttcaggattt caccgcctta tgccgtctga aagacgtttc cgacccatcc ctactacaca
    15121 ctttgtccgc aaacgcatca caggcaagat tatggaagtt atccaatacc caaattcccc
    15181 cttcaaactc caccaaccct tcccgcccgc aggcgaccag cccaccgcta ttgccggctt
    15241 gctcgaaggg ctttcagacg gccttgccta ccaaaccctg ctcggcgtaa ccggttcggg
    15301 caaaacctac accatggcga acgtcatcgc ccaaagcggc cgacccgcca tcatcatggc
    15361 gcacaacaaa acccttgccg cccagcttta tgccgaaatg cgcgagtttt tccctgaaaa
    15421 cgcggtggaa tatttcgtct cctactacga ctattaccaa cccgaagcct atgtgccaag
    15481 ccgcgatttg ttcatcgaaa aagacagtgc gatcaacgaa cacattgaac aaatgcgcct
    15541 ttctgccacc aaaaacctga tgacgcgcga tgacgtgatt atcgttgcca ctgtgtccgc
    15601 catttacggt atcggcgacc cgactgagta tcaacaaatg gtgttgtccg tcaaagaagg
    15661 cgacaccatc gagcagcgcg acatcatcgc cacgctcgtt tccatgcagt acgaacgcgg
    15721 cgatttggat ttcaaacgcg gcagcttccg cgtgcgtggc gacgtgattg acgtgtaccc
    15781 cgccgaaagc tccgaaaacg ccttgcgtat cagcctgttt gacgacgaaa tcgaccgcct
    15841 cgatatgttc gacccgcttt caggcagcct gcaccaacgc gtcggccgct acaccgtctt
    15901 cccgtccagc cactacgtta ccccgcgcga caccgtattg cgcgcctgcg agtccatcaa
    15961 agaagaattg cgcgaacgca ttgatttctt taccaaagaa aaccgtcccg tcgagcaaca
    16021 acgcatcgaa cagcgcaccc gtttcgacct cgaaatgctc tacgaaatgg gcttctgcaa
    16081 aggtatcgaa aactactccc gtcacttctc cggcaaaaaa gaaggcgaac cgccgcccac
    16141 gctgatggac tacctgcccg acaacgccat catgttcatc gacgaaagcc acgttaccgt
    16201 cacacaaatc ggcggcatgt acaaaggcga cgcatcgcgc aagcaaaacc tcgtcgatta
    16261 cggcttccgc ctaccttccg cccgcgacaa ccgcccgctc aaattccacg aatttgaaaa
    16321 agtcatgccg caaaccgtct tcgtttccgc cacccccgcc aaatacgaag aagaacacgc
    16381 cggacaagtg gtcgaacaag tcgtccgccc cacagggctg gtcgaccctc aaatcatcat
    16441 ccgccccgtc gccacccaag ttgatgactt aatgagcgaa atcaacgacc gcatccaaaa
    16501 aggcgaacgc gtactcgtta ccacccttac caaacgcatg gcggagcaac tcaccgacta
    16561 ttacagcgaa ctcggcatca aagggcgcta cctgcacagc gacatcgaca ccgtagagcg
    16621 cgttgaaatt attagagatt tacggctcgg attgtttgac gtactcgtcg gcatcaacct
    16681 cttgcgcgaa ggcttggaca tccccgaagt ctccctcgtc gccatcctcg acgccgacaa
    16741 agaaggcttc ctgcgctccc accgcagcct gattcaaacc ataggccgcg ccgcgcgcaa
    16801 cgtgaacggc gtcgcgattc tgtacgccga caaaatcacc gactctatga aagccgccat
    16861 cgacgaaacc gaacgccgcc gcgaaaaaca gattaaattc aacgaagaac acggcatcgt
    16921 gccgcagcag attaaaaaac aagtcaaaga catcatcgac ggcgtgtacc acgaagaaga
    16981 cagcggcaaa ggtcgtctga aaggcaaaaa caaggttaaa gtcggcgaaa tccacaacga
    17041 agaagacgca attaaagaaa tcgccaaact ggaaaaagcc atgcagcagg cggctaggga
    17101 tttgcagttt gaagaggcgg ctgtgttgcg ggataggatt cgggggatta aggagaattt
    17161 attattttag gatgtaaaaa atgattaaga tgaaagatat ttatgagtca atttgttgta
    17221 aatctattag aaagataatg tatatatcag atagttttgt agctatttca ggaacggggt
    17281 ttattattaa atttcaggaa aaattttatt tttgctgttt atatcattgc atgtctaatg
    17341 aaggaaaatt tgaaaaaaat aaattgtttg ggttaagtat tttatataaa acatacttgg
    17401 attcggattt caagtgcaac actagtgtat tagtggttgg aacagattca agaataaaac
    17461 acttggcgtt tcgtagccaa gtgtttttct tggtcggtgg ttcaactcat cttgaaccct
    17521 gcgtatctcc c
//
LOCUS       DAXHTY010000036        17110 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXHTY010000036 DAXHTY010000000
VERSION     DAXHTY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17110
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.036"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..289)
                     /locus_tag="ACFNMK_001014"
     CDS             complement(<1..289)
                     /locus_tag="ACFNMK_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6364302.1"
                     /translation="MSTFFQQTAQAMIARHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDEL"
     gene            complement(461..1630)
                     /gene="metK"
                     /locus_tag="ACFNMK_001015"
     CDS             complement(461..1630)
                     /gene="metK"
                     /locus_tag="ACFNMK_001015"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC6364303.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGLL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1813..1923)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1976..2872
                     /locus_tag="ACFNMK_001016"
     CDS             1976..2872
                     /locus_tag="ACFNMK_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6364304.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2916..3980)
                     /gene="tsaD"
                     /locus_tag="ACFNMK_001017"
     CDS             complement(2916..3980)
                     /gene="tsaD"
                     /locus_tag="ACFNMK_001017"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC6364305.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGVSYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(4096..5283)
                     /gene="ccsB"
                     /locus_tag="ACFNMK_001018"
     CDS             complement(4096..5283)
                     /gene="ccsB"
                     /locus_tag="ACFNMK_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC6364306.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYESKFAVQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(5276..7228)
                     /locus_tag="ACFNMK_001019"
     CDS             complement(5276..7228)
                     /locus_tag="ACFNMK_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC6364307.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLDVQGFQGKTVSREGGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7505..8128)
                     /locus_tag="ACFNMK_001020"
     CDS             complement(7505..8128)
                     /locus_tag="ACFNMK_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6364308.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPMVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            8334..8963
                     /gene="yihA"
                     /locus_tag="ACFNMK_001021"
     CDS             8334..8963
                     /gene="yihA"
                     /locus_tag="ACFNMK_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC6364309.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(9084..11480)
                     /locus_tag="ACFNMK_001022"
     CDS             complement(9084..11480)
                     /locus_tag="ACFNMK_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC6364310.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11632..12747
                     /gene="pilM"
                     /locus_tag="ACFNMK_001023"
     CDS             11632..12747
                     /gene="pilM"
                     /locus_tag="ACFNMK_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC6364311.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12750..13349
                     /locus_tag="ACFNMK_001024"
     CDS             12750..13349
                     /locus_tag="ACFNMK_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC6364312.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13350..13997
                     /locus_tag="ACFNMK_001025"
     CDS             13350..13997
                     /locus_tag="ACFNMK_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC6364313.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            14015..14560
                     /locus_tag="ACFNMK_001026"
     CDS             14015..14560
                     /locus_tag="ACFNMK_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC6364314.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14579..16864
                     /gene="pilQ"
                     /locus_tag="ACFNMK_001027"
     CDS             14579..16864
                     /gene="pilQ"
                     /locus_tag="ACFNMK_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC6364315.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4601 a         4833 c         3925 g         3751 t
ORIGIN      
        1 tcagttcgtc gaaacggcaa aacaggttga aatcgatgcg ggtaatgagg ctgtgttcga
       61 gttcgggatc ggagaggctg tgccattgtc cgagcaggac ggctttgaac atggacaaca
      121 gcgggtaggc gggacggccg cggtggtctc taaggtaacg ggttttttga cggttcaggt
      181 attgttcgat tggttgccaa tcaatcacct ggtccaactt caatagtggg aaacggtcga
      241 tgtgtctggc aatcatggct tgggcggttt gctggaagaa ggtgctcatg agaaatcccc
      301 taaatgtctt ggtgggaatt taggggattt tggggaattt tgcaaaggtc tctatctgta
      361 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatata aaaatgccgt
      421 ctgaactgtt cagacggcat ttttgatttt caaaccggaa ttacagcccc gctgccgccc
      481 tcaatgcagc agctttgtcg gtgcgctccc aagtgaactc aggttcttcg cggccgaaat
      541 gtccgtaagc ggcggattta ctgtaaatcg ggcgcaagag atcgagcatt tggacgatgc
      601 ctttggggcg caggtcgaaa tgttcgcgaa ctaaggtaat cagtttttcc tcgctgattt
      661 tgcctgtgcc gaaagtgtcg atggaaatcg aagtcggttc ggcaacgccg atggcgtagg
      721 aaacttggat ttggcattgg gttgccaaac cggcggcgac gatgtttttc gcaacgtagc
      781 ggcaggcgta agcggcggaa cgatccactt tggacgggtc tttgccggag aacgcgccgc
      841 cgccgtgcgg agccgcgccg ccgtaggtat caacgatgat tttacggccg gtcaaaccgc
      901 agtcgccttg cgggccgccg atgacgaagc ggccggtcgg gttgatcagg tatttggttt
      961 cgtcggtcag cagttcagac ggcaggacgg gcttgataat ctgctcaatg acggcgtttt
     1021 tcaactcttc ataaccgatt tccgggtcgt gttgcgtaga gaggacgacg gtatcaatgc
     1081 gttttacttt gccggattcg ctatcgtaaa ccacggtcag ttgggctttt gcgtcgggac
     1141 gcagccaagg caggaggccg tctttgcgta attcgctttg acgctgcatc aggcggtggc
     1201 tgtaatagat ggcaaacggc atcagggtcg gggtttcgtc gcaggcatag ccgaacatca
     1261 aaccttggtc gccagcgcct tggttcaagt caatgccttc gccttcgttc acgccttggg
     1321 cgatgtccgg agattgctgg tcgtagtaca cgccgaccgc gcagccgttg gcatcaaagc
     1381 ccagctcgga ggagttgtag ccgatgcgtt tgatggtttc gcgtgcgact ttgatgtagt
     1441 ccacttgggc ggtggtggta atttcgcccg ccaatacgca caagcctgtg ttgaccaagg
     1501 tttctgcggc gacacgcgct ttggggtctt gcgccaagat ggcatccaaa atcgcatcgg
     1561 acacttggtc ggcaacttta tccggatggc cttcggatac cgattcggaa gtaaacagat
     1621 attcgctcat tgagattttc cttgaaaaac aaaccatctt cttcagacgg catgttgtat
     1681 gaacataatg tcgacagcgg gaaatatagc aaaatttccc tattcatacc attcagttga
     1741 gaaatattcc catttgaatg gcactttgga atctctgccc gtacgtttct tacaggcaaa
     1801 aaaattcccg catcaagcgg gtttggattg ctctggtgag ccacatcggc ttttcaaccg
     1861 tccaccttac ttttcctttt gaaaagcagg ttggcatgga attcccaact cttaatgcag
     1921 cgcggatggt agcagaaaag cctttccgcc gcaatacttc cctttttcag acggcatgtt
     1981 tcgtttacaa ttcgggctgt ttcccccttt gcgaaccgcc atgcacatcc tgttgaccgc
     2041 cctgctcaaa tgcctctccc tgctgccact ttcctgtctg cacacgctgg gaaaccggct
     2101 cggacatctg gcgttttacc ttttaaagga agaccgcgcg cgcatcgtcg ccaatatgcg
     2161 tcaggcaggc atgaatcccg accccaaaac agtcaaagcc gtttttgcgg aaacggcaaa
     2221 aggcggtttg gaacttgccc ccgcgttttt cagaaaaccg gaagacatag aaacaatgtt
     2281 caaagcggta cacggctggg aacatgtgca gcaggctttg gacaaacacg aagggctgct
     2341 attcatcacg ccgcacatcg gcagctacga tttgggcgga cgctacatca gccagcagct
     2401 tccgttcccg ctgaccgcca tgtacaaacc gccgaaaatc aaagcgatag acaaaatcat
     2461 gcaggcgggc agggttcgcg gcaaaggaaa aaccgcgcct accagcatac aaggggtcaa
     2521 acaaatcatc aaagccctgc gttcgggcga agcaaccatc gtcctgcccg accacgtccc
     2581 ctcccctcaa gaaggcgggg aaggcgtatg ggtggatttc ttcggcaaac ctgcctatac
     2641 catgacgctg gcggcaaaat tggcacacgt caaaggcgtg aaaaccctgt ttttctgctg
     2701 cgaacgcctg cctggcggac aaggtttcga tttgcacatc cgccccgtcc aaggggaatt
     2761 gaacggcgac aaagcccatg atgccgccgt gttcaaccgc aatgccgaat attggatacg
     2821 ccgttttccg acgcagtatc tgtttatgta caaccgctac aaaatgccgt aacgaaaata
     2881 aaaatgccgt ctgaacaatt tcagacggca ttttgtcatc tgacgatttc cgacagcggc
     2941 cagcgcggac ggacattgaa cgcaccgacc tccctgccct tgcccaggtg catcgcaccg
     3001 gcaaaggcaa tcatggcacc gttgtccgtg cagtatgcca tcggcgggaa aaacacgctg
     3061 actttttcgg acggatgttt cggcttgcct tttggggtcg ggatttgcac cgtcatattg
     3121 ccgaaagttt cacggagttt gcggtttgca ccgaccccgc cggcgaccac tacggttctg
     3181 aaccctgtct gcaacagggc ttttttcact ttcgccgcca acacatcgac taccgcatct
     3241 tgaaacgcac ggcaaatatc gttgcgggtt tgctcgggaa tggtttccga accgtttgcc
     3301 tcacgcactt tttcaacggc agtcaaaaca gcggttttca aacctgaaaa actcatctgc
     3361 aaatcgtcgg aatgaatcat cgggcgcgga aaaacaaacg cttcgggcct gcccgattcc
     3421 gcaagttccg acagtttcgc accgcccgga tacggcaagc ccagcagttt cgccgttttg
     3481 tcgaatgcct cgcccgccgc atcatcgacg ctctcgccca aaagcgcgta gtcgcctatg
     3541 cccctgaccg ccataatctg cgtatgcccg cccgaaacca gcagcgcgac aaaaggaaag
     3601 tcgggttttt cctccgccaa cagcggcgac agcagatgtc cttccaaatg atggacggga
     3661 ataacaggct tgtccaacgc taaagccagc gcgttggcgt agctcgaacc cgccagcagc
     3721 gcaccgccca aaccgggccc ctgcgtaaag gcaaccgcgt caatgtcgcc atacgatacg
     3781 cccgcctgcg ccaaacagcc ttccgtcaac ggaacaaggc ggcggatatg gtcgcggctt
     3841 gccagctccg gcacaacccc gccgtattcg gcgtgcattg ccatctgcgt gtgcaggcag
     3901 tgcgcccgca atcctcgttc ggtgtcatac agcgcaacgc ctgtttcgtc gcaagaagat
     3961 tcgattccca ataccaacat ggtctgatgc cgttaaaaac tgaaaaacgt attctagcgg
     4021 atttcggcac gactgccgta tcccaaaaac ggaacatgcc gtctgaagcc cttcagacgg
     4081 catcgtcgca ccgtatcaaa gcgttccgta agaatgcagc ccgctcaaaa acatattcac
     4141 gccgataaag gcgaatgcgg ttacgaacaa accgataatc gcccaccacg ccagcacttt
     4201 gccgcgccaa ccggcaacca gccgcaagtg cagccaaacg gcgtaattga gccagacgat
     4261 gaacgcccac gtctctttcg gatcccaact ccaatagcgt ccccaagcat ctgccgccca
     4321 cagcgcaccc aaaatggtgg caatggtaaa gaacagaaag ccgacggcaa tcgccttata
     4381 catcacctcg tcgatcaatg ccgacggcgg cagccacagt tttccgcctt ttccttccgc
     4441 acgcagggaa accagttcgg caataccgag catcgcggaa atgcaaaacg cgccgtaacc
     4501 gataaagttt gccggaacgt ggattttcat ccaccaagat tgcaatgccg gaatcagcgg
     4561 ctggatggta tgcgcctcgc gagacacgct gtaccacaag acgaatccaa ccacgaccgc
     4621 cataaagccg aacacgaagc cgcccaattt ctgtacggcg aatttgctct cgtaatacaa
     4681 atacatcagc gcggtaatca ccaaaaacag gatgaacact tcatacaggt tggaaaccgg
     4741 aatatgcccc gcatcgggac ggagcagata gctttcgtgc caacgtacca gcagaccggt
     4801 aaagcctgct acggcagaca cccatgcaaa cacggttccc atacccaaca gcgtgttggt
     4861 cggcacattt tttacgcttg ccaaaaccgc gcccgaaata taggcgaaca gggcgaagaa
     4921 gacaaaggcg cactgccaca tgatcgccga ctggctgctg aggaaatacc gcaacaggaa
     4981 aatctctgcc gatttgatgt tgccgccata cagaccgacg gcggcatagg caagcaatac
     5041 gcttaaagga acaaaccagc gcatcggctt gaaaaaccag cccaaaaata cggcaatacc
     5101 ggcactcgcc cacaacatga ccgtttcgta aatgtccata tgcataccgg aacgggtctg
     5161 tacgaaaacc gtagccgcaa aaaccagcac ggcaaatacc caatcccaaa gattcagatt
     5221 gctgatcaaa gacttctgaa tcagcagctc gtgttccgga agggttttat agtgttcagt
     5281 catgattcaa gtccttgccg agccgttgca gactctcgac gtgttttgga aattccttct
     5341 gcaaatcccg ttcactgcgg gccgaagaca tggcaaaacg gattttgccg tctgaaaaca
     5401 atacccacgc ccgtttttcg cgcacataaa acatcaatac cgtacccaat accaacagca
     5461 ccgagccgag atagaccaaa agcgcacccg gggaacgggt catctgcaaa cccgacgaac
     5521 gcacctcgga aaacccatca agttgcagca gcataggcgc gggatattcg gtcaaacccg
     5581 tgtacgcatc catactgtgc agcaggaaac ggttccgcgc ttcatcctgc tgccattcgg
     5641 gcaagccgta ccggcgtatg gtttcatcca aagcagcgtt catcacgccg taaagcattt
     5701 cgtagaaata accctgcatc ttatcctgct gctctttcgg gatattggac gtaataaatt
     5761 cgtccaatcc caaatagcct ttttgtgcaa agatgttcag cgtgttttcc gcagccagca
     5821 tgaattgttc gcggatttcg gcaggtgcgc ctttggttgc gtcggcaacc agacgtttgc
     5881 gcccttcccc atctttcaaa aactcacgca atgccataaa ggtgtccgct ttcaactgct
     5941 tgtccaaggg gatacgcagc cagcggtatt gctgctgcaa gccgctgcgc gtgccggtaa
     6001 tccaaaaata atcctgttcc tgcaaaaccg gcagcatata gtttttatat tcgaccgcct
     6061 gccctgccgc atcacggata cggtaaacaa tggaagggcc gatattggtg tattttttac
     6121 cttcctgagt aacggcgcgg acatcgttca gcgtggattt caggcttttt tcccgttccg
     6181 cgccctcgct catgtcctcc acattcatag aagtgaactg atcgaactca agacgatatt
     6241 tgtgtttgcc aatttccaac ggaaactggt gtatggatgt tgccttcaac acgacaggct
     6301 cgcgcgaagc atcacccaaa ttccacgcct tgaatgtcaa atccgaaccg ccgtcggcaa
     6361 aactcgcctg ataaatcgtg atgccgtgca aggtcaaagg atggttcacg cggatggtgc
     6421 gctcgagttt ctcaccggtt gccttatccg ttacttcaat atcactggca aaatcgcgcg
     6481 gcatacccgt attgtaaaaa tcgatatgga attttttcag tttgacttca aaaggcaagt
     6541 cctgaaccaa tatcccgttg tcggcattca ggaaaaccac atccgcactc tgcccctcgg
     6601 aaatattgac gttgccccta aatgagagat tggacgcact caaaatactt tcgggcttga
     6661 aatccttggc ataaaccgcc tgattgtccg gaacaatccg accggtcagc atacccagtt
     6721 tcaacagcag gttactgtct atcaacccgc ccaggcaaat gacaatcaaa gcaacatggg
     6781 caaagatata gccccatttg ttcatcgtgc cttttttggc ggcaatcaga accgacccgc
     6841 cctcacggct gacggttttt ccctgaaaac cctgcacatc cagataacgt ttggcaacct
     6901 cgggcgcaat ttttacatcc aacagcgaag aatggcgcat cgccgccaga gatttttctt
     6961 taaccttttc ccgaaaagac ttcatttcgc gccagaacgg cggcacattg cgaatcaggc
     7021 acaaactggt agaaaccacc aaaaacatca tgataacgac aaaccatgcc gaagcataga
     7081 cgtcatacag tcccagaaaa ccaaaaatct gcgcccaaaa aggtccgaat ttgaccaaat
     7141 aatccgtctg cggctggttt tgctgcaaca ccgtaccgat aaccgatgca atacccagca
     7201 gactgagcaa agcgactgca aagcgcatgg agctgaaaaa agcgaaccac ggacgggaaa
     7261 gaagtggggg agatctacgg gatttactca ttgtgtgttt attccgccat caggaatatg
     7321 ggaaagcaga attgggcaaa cagaaaacaa cgtcccgatt ctactgtctt gatgcttttt
     7381 atttcaagac aatgaagaca gcctgcatcg attccaacgg ttgcgattga aaaaacttat
     7441 cgcagaattg cctgaagccg tctgaaaact tttcagacgg cctctaaaac agactattgc
     7501 ggaattaacg caaaccttgg ataaagttgg cgaccgcttt caaatcttct tcagacatac
     7561 ggtttgcaat atcttccatg atggtatttt tacgctgacc ggacttgtag gcattcatct
     7621 gttcaacaat atatgcctga tgctgaccgc ccaaacgcgg ataagcctga atttcgcttc
     7681 cgcctcccgg catacccgca ccgctcggac cgtggcagga catacacgcc ggcacttttt
     7741 tatcgctcaa accgccgcga tagattttcg cgcccaattc gggattttcc ttaggattgg
     7801 cttcaccgga tttgggctgc tgtttggcat agaatgcgga tacgttcaaa atatcctgat
     7861 cgctcaaatt cattaccatc ggtttcatca cagctgccga accgtgggtg cgtttgccgt
     7921 ctcggatacc gatggtttga tgatagatgt aagcagtatg ctgtgccgcc aaacgcggat
     7981 acatcgcaat gccgctgtta ccgtctgctg catggcaagc cgcacaaacc gttgcggcaa
     8041 cctgtttgcc tttttccacg tctgctttgg gagaggcgga aaccgcaccg gcagccaaaa
     8101 caaaggccaa taaagtcaat cgtttcatgg agtgctcctg attacagcat tggataacgc
     8161 aacaatgctc tttttatatt caaatacggg atttttgacc cgattaaaac cgatgattct
     8221 gtaaacgtgt tattctatac taaatttaca ttaaattacc actgtgtttc acataaaacc
     8281 aaccgcatat ttttgctgtc ggacaaacgg cggcggaaaa caaggatatg cccatgaacc
     8341 tttttcaaaa cgccaaattc ttcacgacgg taaaccatct caaagacctg ccggacacac
     8401 cgctcgaaat tgcctttgtc ggcaggagca atgccggaaa atccagtgcc atcaataccc
     8461 tgaccaacca tgtccgtctt gcctacgttt caaaaacacc cggacggacg cagcatatca
     8521 acttcttcga gctgcagaac ggcaatttta tggtcgattt gcccggctac ggttatgccc
     8581 aagtccccga agcagtacgc gcacattggg tcaatctgct cggcgactat ctgcaacagc
     8641 gcaaacagct tatcgggctg gttttgatta tggatgcccg ccatccttta aaagaactcg
     8701 acatccgtat gctggatttt ttccacacga ccggcagacc ggttcacatc ctgctgtcaa
     8761 aagccgacaa attatccaaa aacgaacaga taaaaaccct gtcccaagtc aaaaaactgc
     8821 tcaaacctta ttccgacagg caaaacatca gcgtacagct gttttccagc ctgaaaaaac
     8881 aaggtattga cgaggccaac cgaactgtcg gaagctggtt tgacgcagca gatgccgccg
     8941 cttcctctcc aaaggaaaac tgaccccaat tatacggaaa ccgtatttcc cccacttgac
     9001 cgaccgcaaa catttaaaaa attgccactg ccaaatctaa aatgccgtct gaaaagtctt
     9061 tcagacggca ttttgcggag tctttaaaac agagaatcca actgctgctg tttggaacca
     9121 gtattactcg gaagcaccgg cgtttcctgc atatcttggc ggacttcgtc atccgccgcc
     9181 tgccgtccgc cttctgccgc gcccccgtca tcttcttttg cccgtcggga aggttgcggc
     9241 gcaataccgc tgttgtccag cgtcaagccc ggatcggtta ccatacgttc cttcatatag
     9301 tattcgccat tgctgctgac cacaccttca ggcattttca tccccttgcc ctgctttcct
     9361 ttcaacgcaa aacgcatata gtccacccaa accggcaccg caatcgtacc gccgtagccg
     9421 acacgcccca tactcttagg tttgtcgaag ccgatatata cggcagtaac cacatcaggg
     9481 ttaaaaccga caaaccacgc atccttattg tcattggtcg tacccgtttt accggcaata
     9541 tccgttcttc ccaacgcagc tgcccccctt gccgtaccga cacggaccac atcctgcata
     9601 atcttataca taatataggc attgcgcgga tcgattgcct gaggcgcatt ttgcccagcc
     9661 accaaaggtt gcatttgggc gcgcaacctg ccgtctctgt cataaatctt atcgattacg
     9721 tgcgaagaaa ccctatatcc gccgttcgca aatacgctat atgcctccgc cactttcaac
     9781 ggcgttgtct cgcccgtacc taaagccata gacaggcttg ccggcagctc ggacgacctg
     9841 aagccgaaac gccggatata ctgttgcgcg taaccgacac cgatagacat caaaatacgg
     9901 atggaaacca tattcttgga agccgtcaga gcctgtctca aagtaatgta gccggaatat
     9961 ctgccgtctg aatttttagg tgtccaaacc gaaccgttcg gccctttccc cggcagggaa
    10021 atcggcgcat cgttaaccac cgtggacgcg gtcatcccct tagataatgc cgccgaatag
    10081 acaaacggct taaaggtcga acccggctgc cgcattgcct gaacggcacg attgaatgtt
    10141 ttgctgtgaa aatcataacc gccgaccagc gcgcgcacag ctccggtttt tgcatccagc
    10201 gaaaccaaag ccccctgcag caacggctct tgaaccaccg cccaacgccc gccgttgttt
    10261 ttgacacgga tgaccgcgcc cctgcggata cggtcctccc ccattttttc attattgacc
    10321 gcgcgggccg caaagcccaa ggcgcgccta tcaagcgtaa cccgcctgcc gccgggcagc
    10381 tgtatgacga catttttctt tttagtcaca tccaacacaa cggcgggaac cattttatcg
    10441 acggtataga gtcccgacag atactggctg acagtctcct cgacatcttc actcttactc
    10501 aaatcgatat agttttccgc accgcggtag ctgctgccgc gatcgaaatt ccgtagagcc
    10561 ttgcgcaatg cctcggttgc caccttctga tgatcggcgc ggaccgtggt ataaacctta
    10621 aaaccctgcg tataggcatc ttcaccgtat ttctcataca gttcctgacg caccatttcc
    10681 gccacatata aagcactctg atcgattttc cgaacaaacc gctcgtaatg cagttcctca
    10741 ttcaacgcct gatcgcgctg ttgcacggta atcatcttct cctcgagcat attgttcaaa
    10801 atatacttct ggcgcaactt ggcacgttcc ggattaacaa tcggattata ggcagacgga
    10861 gccttgggca gtcccgcaag catggcggct tccgccaaag tcaaatctcg gacattctta
    10921 ttgaaataga tttgcgcggc agatgcaaaa ccataggcgc gctgaccgag gtaaatctga
    10981 ttgaaataca actcgaggat tttgtctttg cttaaagact gctcgatttt ataggcaagc
    11041 aacacctcat tgaatttgcg tgtgaacgtt ttttcactgc tcaaataaaa atttttcgcc
    11101 acctgctgcg taatcgtact cgcacccgac tgcacgctgc cggacacgac attgccgacg
    11161 gcagcgcggg caacacccca aacatccacc ccccaatgcc ggtaaaagcg tttatcctcg
    11221 gcggcgataa ccgcattccg caacacctct gggaaatcgc cgatttttgt aaattcgcgc
    11281 cgctgctccc catacatacc gatgacttcc ccatccgccg aataaatagt caacggcatt
    11341 ttaggctggt aatgctgcaa agaatccaaa gacggcagtt tcggatacgt taccaaaata
    11401 gcaatggcaa ccaaacccac tccaaataca caaaacccaa aaaccaaacc aaaacaagtc
    11461 gttaaaatct ttttaatcat agctgaataa taatttacca ttattggtat taaataaagt
    11521 aaaatagcaa ccgatttcta caaagcacgg tttcaatgtg caaagaacaa ggaatccatt
    11581 acggataccg aaacggttac tcactgtaca aataaagcag gaactttcat catgcgcttg
    11641 tttaaaagct tgaaaaaccc taaaaaaaca gatgccaagc tccctaaaaa atcttcggga
    11701 ctcaataacc gcgcggcaat cggcatcgat atcgaccagc attccatcaa aatggtccaa
    11761 ttgtcaggac gtagtttaaa ccaaattcaa ttggaaaaat acgtcattgc caaattacca
    11821 aagaatatca ttcaaggcaa taaagtccaa aattacgatc aacttgttac atatttgcaa
    11881 caagcctatg ccaaactggg tacttcgtgc aaaaacatca tcgcgtccgt cccgcaaaat
    11941 ctggcaacca tcgaacaatt gacctacaca gacaaagatg cagaattaga cctgcagggg
    12001 ttcgtggagt cctccatctc cgaagtcagc tcgatatcgc tcgaagaagc caattacgac
    12061 tatcaggtct tgtcccaatc ggccgccggc gaagctgtgt tggccgtcgc atcgagaaag
    12121 gatgaaatcg aacccctgat tgacgcattc aacgccgccg gtatgaaatt atccgcgctt
    12181 gatgtggaca ttttcggaca atacaacgcc tacgcgctat ggataaacca tttcgccccc
    12241 gagcttgcag ccgaaaaagt cgccattttc ggcgtatatg ccgcacagac ctacgccttg
    12301 gtcatccaag acggaaaaat cctatacaaa caggaaacct ccgtcagcga agaacagctc
    12361 aaccaactca tccagcgcac ctatcaggta acagaagaaa aagcggaaga aatcatcaac
    12421 tccccgcaaa aaccttccga ttaccaagaa agcgtggcaa actatttcaa ccagcagatt
    12481 acccaagaaa tacaaagggt cttgcagttt tattacacca cgcagaccgc agacgatatg
    12541 accgacatca agcatatcct gctgaccggg gaagcggcgc gccaggaagg tatcgcccaa
    12601 accgtcgcct cacaaaccaa tgcagatgta caatgcgtcc atcccgcgcg ttattttgcg
    12661 gacaacctca aaacagacaa acaacaattc gaacttgatg cgccgacact gaccagggcg
    12721 ttcggtttgg cggtacgggg attataatta tgaacaattt aatcaaaatc aacctcctcc
    12781 cctacaggga agagatgaac aagcgcaaac agcagcagtt taaaacgctg atgtacggtg
    12841 ccgtgctgac gggcgttgcc gccgttgcgg caacctacct gtttatcgac aatatgatca
    12901 ataaccagtc ggaaagaaac acgctgctgg aaacctccat cgcacacttg gataccgagc
    12961 tgtcggaaat acaaaagctc aaacaggaaa aagatgcctt cctgattaag aaaaacaaaa
    13021 tcgaggagct ccagctcaaa cgcctccaag ccgcaaaaat cctcgacagc ctgaatgagg
    13081 ccgtccccgg aagcacctac ctgacctcgc tggatgccgt taccgccgac tcttatcggc
    13141 tcagcggcag gacatccagc gacaaccgcg ttgccgccat gatgagggcg atgcccaata
    13201 ccggcatatt caagcaaccc gaattgttaa gcatcaagaa aaacaattcg catcaagaat
    13261 ttacccttca ggcaacatta caacccatcg taaaggcggc cgaatccaaa gagaatccgg
    13321 cttcgggaaa cgcacaggag gcaaactgaa tggcttctaa atcatctaaa accaacttgg
    13381 atctcaacaa ccttcacctg ctcaaccttc ctgccaggct ttttatcgcc ctgctggccg
    13441 ttgccgccgt gctggggctc ggttatgccg gattgttcaa aagccagatg gaatcccttg
    13501 aggaatacga agcaaaagaa accgaactga aaaacaccta caaacagaaa agtatcgacg
    13561 cggccagcct gaacaacctg agggacgaac ttgcctcaat ccgctctgcc ttcgatatca
    13621 tgttgaaaca gctgccgaca gatgcagaaa ttcccaacct ggttcaagag cttcatcagg
    13681 caggttcgag caacggcctg cgcttggaca gcgttatgcc ccaacctccc gtagatgacg
    13741 gccccatcaa aagattaccc tattccattt ccattaccgg aaattacgaa cagatcagcc
    13801 aatttacccg cgatgtcggc agcctctccc gaatcattac ccttgagtcg ctgaaaatcg
    13861 cccaatctcc ggaaaacggc ggcaatcctg acggcaagag cagcatcctg aacctcagtg
    13921 ccattgccac cacctaccaa gcaaaatccg tagaagagct tgccgcagaa gcggcacaaa
    13981 atgccgagca aaaataactt acgttaggga aaccatgaaa cactatgcct tactcatcag
    14041 ctttctggct ctctccgcgt gttcccaagg ttctgaggac ctaaacgaat ggatggcaca
    14101 aacgcgacgc gaagccaaag cagaaatcat acctttccaa gcacctaccc tgccggttgc
    14161 gccggtatac agcccgccgc agcttacagg gccgaacgca ttcgacttcc gccgcatgga
    14221 aaccgacaaa aaaggggaaa atgcccccga caccaagcgt attaaagaaa cgctggaaaa
    14281 attcagtttg gaaaatatgc gttatgtcgg cattttgaag tctggacaga aagtctccgg
    14341 cttcatcgag gctgaaggtt atgtctacac tgtcggtgtc ggcaactatt tgggacaaaa
    14401 ctacggtaga atcgaaagca ttaccgacga cagcatcgtc ctgaacgagc tgatagaaga
    14461 cagcacgggc aactgggttt cccgtaaagc agaactgctg ttgaattctt ccgacaaaaa
    14521 caccgaacaa gcggcagcac ctgccgcaga acaaaattaa gaagaggatt actccattat
    14581 gaataccaaa ctgacaaaaa tcatttccgg tctctttgtc gcaaccgccg cctttcagac
    14641 agcatcggca ggaaacatta cagacatcaa agtttcctcc ctgcccaaca aacagaaaat
    14701 cgtcaaagtc agctttgaca aagagattgt caacccgacc ggcttcgtaa cctcctcacc
    14761 ggcccgcatc gccttggact ttgaacaaac cggcatttcc atggatcaac aggtactcga
    14821 atatgccgat cccctgttga gcaaaatcag tgccgcacaa aacagcagcc gtgcgcgtct
    14881 ggttctgaat ctgaacaaac cgggccaata caataccgaa gtacgcggga acaaagtttg
    14941 gatattcatt aacgaatcgg acgataccgt gtccgccccc gcacgccccg ccgtaaaagc
    15001 cgcgcctgcc gcaccggcaa aacaacaggc tgccgcaccg tctaccaagt ccgcagtatc
    15061 cgtatccaaa ccctttaccc cggcaaaaca acaggctgcc gcaccgttta ccgagtccgt
    15121 agtatccgta tccgcaccgt tcagcccggc aaaacaacag gcggcggcat cagcaaaaca
    15181 acagacggca gcaccagcaa aacaacaggc ggcagcacca gcaaaacaaa ccaatatcga
    15241 tttccgcaaa gacggcaaaa atgccggcat tatcgaattg gctgcattgg gctttgccgg
    15301 gcagcccgac atcagccaac agcacgacca catcatcgtt acgctgaaaa accataccct
    15361 gccgaccacg ctccaacgca gtttggatgt ggcagacttt aaaacaccgg ttcaaaaggt
    15421 tacgctgaaa cgcctcaata acgacaccca gctgattatc acaacagccg gcaactggga
    15481 actcgtcaac aaatccgccg cgcccggata ctttaccttc caagtcctgc cgaaaaaaca
    15541 aaacctcgag tcaggcggcg tgaacaatgc gcccaaaacc ttcacaggcc ggaaaatctc
    15601 ccttgacttc caagatgtcg aaatccgcac catcctgcag attttggcaa aagaatccgg
    15661 gatgaacatt gttgccagcg actccgtcaa cggcaaaatg accctctccc tcaaagacgt
    15721 accttgggat caggctttgg atttggttat gcaggcacgc aacctcgata tgcgccaaca
    15781 agggaacatc gtcaacatcg cgccccgcga cgagctgctt gccaaagaca aagccttctt
    15841 acaggcggaa aaagacattg ccgatctggg cgcgctgtat tcacaaaact tccaattgaa
    15901 atacaaaaat gtggaagaat tccgcagcat cctgcgtttg gacaatgccg acacaaccgg
    15961 aaaccgcaat acgcttgtca gcggcagggg cagcgtgctg atcgatcccg ccaccaatac
    16021 cctgattgtt accgataccc gcagcgtcat cgaaaaattc cgcaaactga ttgacgaatt
    16081 ggacgtaccc gcgcaacaag tgatgattga ggcgcgtatc gtcgaagcgg cagacggctt
    16141 ctcgcgcgat ttgggcgtta aattcggcgc gacaggcaag aaaaagctga aaaatgatac
    16201 aagcgcattc ggctgggggg taaactccgg cttcggcggc gacgataaat ggggggccga
    16261 aaccaaaatc aacctgccga ttaccgctgc cgcaaacagc atttcgctgg tgcgcgcgat
    16321 ttcctccggt gccttgaatt tggaattgtc cgcatccgaa tcgctttcaa aaaccaaaac
    16381 gcttgccaat ccgcgcgtgc tgacccaaaa ccgcaaagag gccaaaatcg aatccggtta
    16441 cgaaattcct ttcaccgtaa cctcaatcgc gaacggcggc agcagcacga acacggaact
    16501 caaaaaagcc gtcttggggc tgaccgttac gccgaacatc acgcccgacg gccaaatcat
    16561 tatgaccgtc aaaatcaaca aggactcgcc tgcgcaatgt gcctccggta atcagacgat
    16621 cctgtgtatt tcgaccaaaa acctgaatac gcaggctatg gttgaaaacg gcggcacatt
    16681 gattgtcggc ggtatttatg aagaagacaa cggcaatacg ctgaccaaag tccccctgtt
    16741 gggcgacatc cccgttatcg gcaacctctt taaaacacgc gggaaaaaaa ccgaccgccg
    16801 cgaactgctg attttcatta ccccgaggat tatgggtacg gccggcaaca gcctgcgcta
    16861 ttgatgcgtc aaaataaggg catatgtttt acggcatatg ccctttcttt atgctttttg
    16921 ccgcgaccga aatgccgtca ttcccgcgca ggcagaaatc caacttgtcc ggttccggtt
    16981 gtttttcgtc tcgtaacttt tgagccgtca ttcccgcgca ggcagaaatc cagtccgttc
    17041 agtttcggtc atttccgata aattcctgtt gcttttcatt tctggattcc cacttttgtg
    17101 ggaatgacgg
//
LOCUS       DAXHTY010000037        17044 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXHTY010000037 DAXHTY010000000
VERSION     DAXHTY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17044
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.037"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(60..1616)
                     /gene="dnaA"
                     /locus_tag="ACFNMK_001028"
     CDS             complement(60..1616)
                     /gene="dnaA"
                     /locus_tag="ACFNMK_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC6364316.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVCYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMRNLPHEPRQAAGSASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            1908..2042
                     /gene="rpmH"
                     /locus_tag="ACFNMK_001029"
     CDS             1908..2042
                     /gene="rpmH"
                     /locus_tag="ACFNMK_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC6364317.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            2045..2410
                     /gene="rnpA"
                     /locus_tag="ACFNMK_001030"
     CDS             2045..2410
                     /gene="rnpA"
                     /locus_tag="ACFNMK_001030"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC6364318.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            2475..2696
                     /gene="yidD"
                     /locus_tag="ACFNMK_001031"
     CDS             2475..2696
                     /gene="yidD"
                     /locus_tag="ACFNMK_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC6364319.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            2869..4506
                     /gene="yidC"
                     /locus_tag="ACFNMK_001032"
     CDS             2869..4506
                     /gene="yidC"
                     /locus_tag="ACFNMK_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC6364320.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSSKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGECSIDI
                     KRRNDKLYSASVSVPLAAIPAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMVAQTYLNPPPTDPMQAKMMKIMPLIFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(4587..5312)
                     /locus_tag="ACFNMK_001033"
     CDS             complement(4587..5312)
                     /locus_tag="ACFNMK_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC6364321.1"
                     /translation="MSEGMVSPVLYLIPTPLGTPDTPCLLPHEQRAVVGLTDFVVEAE
                     KTARAHLKHLGVTTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSIGIVSEAGCPAVA
                     DPGANLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLK
                     ALEQRSRQQNETQLFIETPYRNDALLADAVENLHPETRLCVATDLTLPTQEIISQTIA
                     QWRKRKEMPNLKKRPTIFVMYAG"
     gene            complement(5359..5949)
                     /locus_tag="ACFNMK_001034"
     CDS             complement(5359..5949)
                     /locus_tag="ACFNMK_001034"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC6364322.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAHKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            6097..6600
                     /locus_tag="ACFNMK_001035"
     CDS             6097..6600
                     /locus_tag="ACFNMK_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC6364323.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSS"
     gene            6634..6813
                     /gene="rpmF"
                     /locus_tag="ACFNMK_001036"
     CDS             6634..6813
                     /gene="rpmF"
                     /locus_tag="ACFNMK_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC6364324.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            6988..7515
                     /locus_tag="ACFNMK_001037"
     CDS             6988..7515
                     /locus_tag="ACFNMK_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC6364325.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            7603..8658
                     /gene="plsX"
                     /locus_tag="ACFNMK_001038"
     CDS             7603..8658
                     /gene="plsX"
                     /locus_tag="ACFNMK_001038"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC6364326.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSASLSKIEQGVAEQL
                     AALEAAKAVQNEIAADL"
     gene            8796..9068
                     /locus_tag="ACFNMK_001039"
     CDS             8796..9068
                     /locus_tag="ACFNMK_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC6364327.1"
                     /translation="MKTLEKRMKALDKRMMKLGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDIRLTEQESQEISFIDKEFRTESTLDYPIKSTG"
     gene            9074..9490
                     /locus_tag="ACFNMK_001040"
     CDS             9074..9490
                     /locus_tag="ACFNMK_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364328.1"
                     /translation="MKTSTVVFGGFFITDNGERIQIPVLENPGIKEINHFFSISNFEK
                     KAGVLVFRIIPEPEFGNTELTIYFEKGYYLPMIQTILEGGDIEVKNLKTENYSGNTME
                     IWGDSYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            9510..9713
                     /locus_tag="ACFNMK_001041"
     CDS             9510..9713
                     /locus_tag="ACFNMK_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364329.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            9740..10702
                     /locus_tag="ACFNMK_001042"
     CDS             9740..10702
                     /locus_tag="ACFNMK_001042"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC6364330.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            10749..11156
                     /locus_tag="ACFNMK_001043"
     CDS             10749..11156
                     /locus_tag="ACFNMK_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC6364331.1"
                     /translation="MKRQAFFKPIACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLILIYSGLNLNQYGVASPCRTICTVCGFVALS"
     gene            11250..12176
                     /gene="fabD"
                     /locus_tag="ACFNMK_001044"
     CDS             11250..12176
                     /gene="fabD"
                     /locus_tag="ACFNMK_001044"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC6364332.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LNFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKAICAEAAQGEVVEAVNFN
                     SPGQIVIAGNAAAVERAMTAAKEAGAKRALPLPVSVPSHCSLMKPAAEKLAEALKSVV
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGITESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQIAAFIEAH"
     gene            12314..14179
                     /gene="msbA"
                     /locus_tag="ACFNMK_001045"
     CDS             12314..14179
                     /gene="msbA"
                     /locus_tag="ACFNMK_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC6364333.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLDLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAINRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGESEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            14279..15844
                     /gene="guaA"
                     /locus_tag="ACFNMK_001046"
     CDS             14279..15844
                     /gene="guaA"
                     /locus_tag="ACFNMK_001046"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC6364334.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSGLTRGIQDDAPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     TEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            15906..16652
                     /gene="fabG"
                     /locus_tag="ACFNMK_001047"
     CDS             15906..16652
                     /gene="fabG"
                     /locus_tag="ACFNMK_001047"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC6364335.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQAFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            complement(16914..17044)
                     /locus_tag="ACFNMK_001048"
                     /pseudo
     CDS             complement(16914..17044)
                     /locus_tag="ACFNMK_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         4309 a         5210 c         3999 g         3526 t
ORIGIN      
        1 ctatatcttt acccttcgga cggcatgggc aatatcatgt cgtctgaaaa cgttttccat
       61 cagttttgaa tcagaatcag cagcttttca taatcctgag ccaattccgg atcttcttcg
      121 cgcagtttcg ccactgccct gatgccgtgc ataacggtcg tatgatcgcg cccgccaaac
      181 gaatcgccga tagacggcag gctcaaagtg gtcagttctt tggtcaggct catcgccacc
      241 tggcgcggac gggcaatgtt gcgcgtgcgt tttttgccga gtacgtcgct gattttgatg
      301 cggtaatatt tggcaacggc atcaataatg atatcggcgg taatgacctt atgcttctcg
      361 gcaataatgt cctgcaaagc ggtacgcgcc aaatcgatgt cgatgacggg acggttcata
      421 aagcggctgc tcgctccgac acggttaaac gcgccttcaa gctcgcgcac gttggaacgg
      481 atcagattgg caatgaacag cgcggcttcg tcttcaatac tgatgcccgc cgcttccgcc
      541 tttttctgca aaatggcgat gcgcatttcc aattcgggcg gctcgagttc caaagtcagt
      601 ccccatgaaa aacgggattt gaggcggtcg tccatgcctt cgattttggc gggtaaaaca
      661 tcgcaagtga ggatcagctg ttttttctcg ttgtggaaat ggttgtacag atagaaaaac
      721 tcttccatcg tacggtcttt tcctttaata aactgaatat cgtcgataat cagcaggtcg
      781 tattgcttgt attgctgctt gaatacgtca taggtattgt tgcgaaccgc cttcataaag
      841 ctgcggatat agtcgtccga atgcatatag cgcactttgg catcgggacg gtttttcaac
      901 agttcgttgc cgaccgcctg cacaaggtgg gttttaccca aacccgtact gccatagagg
      961 aagaacgggt tataactctg tcccggactt tccgcaatcg cctgcgcggc ggcagctgca
     1021 aggcggttgc ccttaccttc gaccaatgtg tcgaacgtat agtccggcga cagattggtc
     1081 tgctcgtaac gcgcctcttc cgcatcgcgc tgtgcatccg tccgtgcttt ggcaactgcc
     1141 gccgattccg gccgggaagc agacccggca gcctgacgcg gctcgtgcgg caggtttctc
     1201 atacgttctg ccaaaatatc cgccgccgtt ttcgacgcag cgggtttgac aggctcttca
     1261 gacggcagct cgtccaacag aacctcctgc acaggcatct cctccgaccc cgcgtgcaag
     1321 gacggctcgg caggttcgac agcaccttca accgccgcca tctcataaca cacgccttct
     1381 cccggtttga atacgaaggc ggaacggccg gcagccaact cttccctcac cgcttctatt
     1441 tttccggcaa actggctctt gagcatattg caggcaaact ggttcttgcc gtacaccacc
     1501 catacgccac cctcctcacc gacggtaagg ggcgcaatcc attgcgcaaa ctgcccttga
     1561 ggcaacatat cgtgaagacg gcggaggcac agcggccaaa actctgctaa tgtcatggat
     1621 aggctcgaat cggtaaaaat gaaatcgaaa acaaagaaaa tataatattt tcaaaaagaa
     1681 aacaaatctg ttgaacgcac atcggttcaa aacgcgactg cccgattata ccgactcacg
     1741 aatattttat ccacaacccg tgcaaaaatt tatccacaga aaggcggcga aaatccgcag
     1801 gcaatcgggc aatcttcctg caaagtttct atattgattg acaaaagcgg caaattggag
     1861 tgtaattcac ggtttaatta tctacccatt ctattttagg aaacatcatg aaacgcactt
     1921 atcaaccttc cgttaccaaa cgcaaacgca cccacggctt cctggtgcgc tccaaaacac
     1981 gcggcggccg cgcagtattg gccgcacgcc gtgccaaagg ccgcaaacgc ctggcggtat
     2041 aattttggac taccgcttcg gaaggcagta ccgcttgtta aaaacggatg atttttcatc
     2101 cgtttttgca ttcagaaacc gccgcagccg cgacctgctg caagtttcgc gttcaaacgg
     2161 caacgggctg gaccatcccc gcatcggtct ggtggtcggc aaaaaaaccg ccaaacgcgc
     2221 caacgaacga aattatatga agcgcgtcat ccgcgactgg ttcagattga acaaaaaccg
     2281 gctgccgccg caggatttcg tcgtgcgcgt ccgccgtaaa ttcgacaggg ctaccgcaaa
     2341 acaggcaagg gcggaactgg cacaactcat gttcggcaac cccgcaaccg gatgcaggaa
     2401 acaggcatga tcagaacggt actctgcagg caaggttcag acggcaacgg gtttcccata
     2461 caaggaacat cccgatgaac ttcctattgt ccaaactcct gctgggactg atacggttct
     2521 accaatattg catcagcccg ctgattccgc cgcgctgccg ttatacgccg acctgttcgc
     2581 aatacgcggt cgaagcggtc aaaaaatacg gcgcattcaa aggcggccgg ctcgccatca
     2641 agcgcattgc acgctgccac cctttcggcg gacacggaca cgaccccgtc ccctgaaccg
     2701 ccccgccgcc cgaactgcac gctttccttt tatttcccat cggtttctat ataatgccgt
     2761 ctgaaacttc gggctggcgg cacgaccgtc gggtatgaag cccgccctta ttccccgtct
     2821 atcggaacac gcaacctgcg gcatttccga ccattcagga aactctatat ggattttaaa
     2881 agactcacgg cgtttttcgc catcgcgctg gtgattatga tcggctggga aaagatgttc
     2941 cccactccga agcccgtccc cgcgccccaa caggcagcac aacaacaggc cgtaaccgct
     3001 tccgccgaag ccgcgctcgc gcccgcaacg ccgattaccg taacgaccga cacggttcaa
     3061 gccgtcattg atgaaaaaag cggcgacctg cgccggctga ccctgctcaa atacaaagca
     3121 accggcgacg aaaataaacc gttcatcctg tttggcgacg gcaaagaata cacctacgtc
     3181 gcccaatccg aacttttgga cgcgcagggc aacaacattc taaaaggcat cggctttagc
     3241 gcaccgaaaa aacagtacag cttggaaggc gacaaagttg aagtccgcct gagcgcacct
     3301 gaaacacgcg gtctgaaaat cgacaaagtt tatactttca ccaaaggcag ctatctggtc
     3361 aacgtccgct tcgacatcgc caacggcagc ggtcaaaccg ccaacctgag cgcagactac
     3421 cgcatcgtcc gcgaccacag cgaacccgag ggtcaaggtt actttaccca ctcttacgtc
     3481 ggccctgttg tttatacccc tgaaggcaac ttccaaaaag tcagcttttc cgatttggac
     3541 gacgatgcca aatccagcaa atccgaagcc gaatacatcc gcaaaacccc gaccggctgg
     3601 ctcggtatga tcgaacacca cttcatgtcc acctggatcc tccaacccaa aggcggacaa
     3661 agcgtttgcg ccgcaggcga atgcagcatc gacatcaaac gccgcaacga caagctgtac
     3721 agcgcaagcg tcagcgttcc ccttgccgcc atcccggccg gcgcgaaagc cgaaaccgcc
     3781 gtcaacctct acgccggccc gcagaccaca tccgtcatcg caaacatcgc cgacaacctg
     3841 caactggcca aagactacgg caaagtacac tggttcgcct cccccctctt ttggcttttg
     3901 aaccaactgc acaacatcat cggcaactgg ggctgggcga ttatcgtttt aaccatcatc
     3961 gtcaaagccg tactgtatcc attgaccaac gcctcttacc gctctatggc gaaaatgcgt
     4021 gccgccgcac ccaaactgca agccatcaaa gagaaatacg gcgacgaccg tatggcgcaa
     4081 caacaggcga tgatgcagct ttacacagac gagaaaatca acccgctggg cggctgcctg
     4141 cctatgctgt tgcaaatccc cgtcttcatc ggattgtatt gggcattgtt cgcctccgta
     4201 gaattgcgcc aggcaccttg gctgggttgg attaccgacc tcagccgcgc cgacccctac
     4261 tacatcctgc ccatcattat ggcggcaacg atggtcgccc aaacttatct gaacccgccg
     4321 ccgaccgacc cgatgcaggc gaaaatgatg aaaatcatgc ctttgatttt ctccgtcatg
     4381 ttcttcttct tccctgccgg tctggtattg tactgggtgg tcaacaatct cttaaccatc
     4441 gcccagcaat ggcacatcaa ccgcagcatc gaaaaacaac gcgcccaagg cgaagtcgtt
     4501 tcctaaatgc cgcagcatga aaaatgccgt ctgaaacctg ttcagacggc atttttattg
     4561 tccaccccct atcgggacgg aaatcttcaa cccgcataca tcacaaaaat cgtcgggcgt
     4621 tttttcaaat tcggcatttc ttttcttttt cgccactgag caatcgtctg gctgatgatt
     4681 tcctgagtcg gtaaggtcaa gtccgtagcc acacataagc gcgtttcagg atgcaggttt
     4741 tccaccgcat ccgccaacaa cgcatcgttg cgatatggcg tttcgataaa cagctgcgtt
     4801 tcattctgct ggcgcgaacg ttgttccaaa gccttcaaac tctgaatccg ctcatttttt
     4861 tcagacggca aataaccctt aaacgcaaaa ctctgcccat tcgcacccga agccatcagc
     4921 gccagcaaca agctggacgg accgaccaaa ggccgtactt caaaaccgtg tttatgcgcc
     4981 aatgccacca aattcgcacc cggatcagcc acagccgggc aacccgcctc actgacaatg
     5041 cctatactgc gcccttcttg caaaggtttc agcaattccg gcaaagtctt caaatccgtg
     5101 tgttcattca atgtttgcag attcagctcg cggataggcg tagtcacgcc caaatgtttc
     5161 aaatgcgcgc gcgccgtttt ttccgcttcc acgacaaaat ccgtcagccc gacaaccgcc
     5221 cgttgttcat gcggcaacag gcacggcgta tcgggcgtac ccaaaggcgt aggaatcaaa
     5281 tacaaaacag gagacaccat tccctcactc atcggttaaa aatgccgtct gaacccttca
     5341 gacggcataa acgggcagtt acagaacctc cacgccctca tttttcaaaa aatcaaccag
     5401 acggaaaacc ggcaaaccga ttaaagcatt cggatcggta ctctcaatcc tttcgatcaa
     5461 caatgcaccc aaagcctcgc tcttcaacgc acacgagcaa taaaccgcat caggctcgcg
     5521 ctccaaatag cggaggatgt gtagctcgtc caacttcctc atcacgacca ccgtcttatc
     5581 gatatgccgc tgcatcctgc ccgtaaccgt atttagcaga acgaccgcgc tgtaaaactc
     5641 aatctccctg ccgctcaaat gcatcaacat cttgtgcgca ttagcaaggt tcatcggctt
     5701 gccccactgc ctgccgtcgc accacgccac ctggtccgca ccgacaatca acgcctctgg
     5761 gaaacgcccg gccaacgacc gcgccttacc ctcggcaagg cgcaatgccg tctgaggggc
     5821 agactccccc aacatcggcg tttcgtcaaa atcgggggac gccgcctgaa aggcaatgcc
     5881 gagcctttcc atctgttcgc ggcggaaaac cgaactcgta cccaaaatca aaggcagttc
     5941 caaacccatc ccatcctcct taccgttgaa aacacgcccg aaggggcggt aaaatccagc
     6001 catgcgccga aacacggata cccgccttcg gcgtaccgca acatttttct taaaaatatt
     6061 gacgttagaa catctaaatt atatcatatc tcgtttatgt cagaccctaa tttgattgac
     6121 ccggaaattt tcgccgccga agggcagaat ctgcaaggca gttttctgct ggaagaattg
     6181 gatgaacgcg tcagttcgca cgattatccc gccgacaggc agaccaaaat atcgtttaca
     6241 ctgaccggcg gtcgcgatcg gctgcaacgc ctgttcctcg acctgaacgt caaagccgat
     6301 atgcccctga tttgccagag atgtatcaaa cccatgccgt tcatgcttga tgaaagcagc
     6361 cgtatcgtcc tgttttccaa cgaagagtcc ttggacgaat ccatgcttgc cgacgaagaa
     6421 ctcgaaggca tactgattga aaaagaactc gacgtgcgca cattggtaga agaccaaatc
     6481 ctgatgtccc tgcccttttc gccgcgacac gaagactgcg gcgacaatgg gacactggaa
     6541 gaagtcaatc gggacaaacc caaccccttt gctgttttgg caggtttgaa aagcagttaa
     6601 ttaggacaca gtttatttat ctaggagctt gaaatggccg ttcaacaaaa caaaaaatcc
     6661 ccttccaaac gcggtatgca ccgttcgcac gacgcgctga ccgcgcctgc actgtctgtc
     6721 gacagcacaa ccggcgaagt acaccgcccg caccacatct cccccaacgg tatgtaccgc
     6781 ggccgcaaag tggtcaaagc caaaggcgaa taatccctat tcgactgact gaaaaagcca
     6841 gaacattgcc atgcaattac tggctttttt tgcattggac gcaccatccg tccaaacttt
     6901 cgccatacgt caacacacag gggcaaagcg ttccgtataa taccccgtga aaatattcca
     6961 aaagccccaa ccaccaagga aattccgatg aaacagaaaa tctggtacac ctacgatgac
     7021 atccaccgcg tcatcaaagc attggcagaa aaaatccgga acgccgacat caaatacgat
     7081 gccatgattg ccatcggcgg cggcggcttt attccggcac gtatgctgcg ctgttttctg
     7141 gaaattccga tttatgccgt aaccaccgcc tattacgaca gcgacaacga aggacaggtt
     7201 accgaagaag tcaaaaaagt ccaatggcta gaccccgttc cagaagccct gcggggcaaa
     7261 aacgtactcg tcgtcgatga agtggacgac agccgcgtaa ccatggaatt ttgcctgaca
     7321 gagctgctca aagaagactt cggtacgatc ggcgtcgccg tactgcacga aaaaatcaaa
     7381 gccaaagcag gcaaaatccc cgaaggcatt ccctatttca gcggcatcac cgtagaagac
     7441 tggtggatca actatccgtg ggacgcactc gacatcgacg aacacaaccg ccttgccgag
     7501 gccggccgag gctgaccctt tcagacggca tattttccga accgatgccg tctgaagccc
     7561 gcacgacccc tgccgcagac cgaaaaccta ccggagaacc ctatgattac acttgccgta
     7621 gatgccatgg gcggcgacca aggacttgcc gttaccgtac ccggcgcaac cgcattcctc
     7681 caagcacacc ccgatgtccg cctgattatg accggcgacg aaacgcaact gcgccaagcc
     7741 ctgaccgcgg caggcgcacc gatggaacgc atcgacatct gccataccac ccaagttgtc
     7801 ggcatggatg aatcccctca gtccgcccta aagaacaaaa aagactcctc catgcgcgtc
     7861 gccatcaacc aagttaaaga aggcaaagcc caagccgccg tatccgcagg caacacaggc
     7921 gcgctcatgg caaccgcacg tttcgtcctc aagaccatac ccggcatcga acgccccgcc
     7981 atcgccaaat tcctcccgtc cgacaccgac catgttaccc ttgcactcga cctcggcgcg
     8041 aacgtcgact gcacgcccga acagctcgcc caatttgccg ttatcggcag cgaactcgtc
     8101 cacgcactcc atcctcaaaa aggacagccg cgcgtcgggc tggtcaacgt gggcacggaa
     8161 gacatcaaag gtacggacac cgtcaaacaa acctacaaac tgctgcaaaa cagcaaactc
     8221 aactttatcg gcaacatcga aagcaacggc atcctgtacg gcgaagcaga tgtcgtcgtc
     8281 gccgacggct ttgtcggcaa cgtcatgctc aaaaccatcg aaggcgcggt caaattcatg
     8341 agcggagcca tccgccgcga attccaaagc aacctgttca acaaacttgc cgccgttgcc
     8401 gccctgcccg ccctcaaagg gctgaaaaac aaactcgatc cgcgcaaatt caacggggcc
     8461 atcctgctcg ggctgcgcgg catcgtgatt aaaagccacg gcggcacaga cgaaaccggt
     8521 ttccgctatg ccctcgaaga agcctaccat gaagccaagt ctgccagcct ttccaaaatc
     8581 gaacaaggcg tagccgaaca gctcgccgca ctcgaagctg ccaaagccgt ccaaaacgaa
     8641 atcgccgccg acttgtaaca cgcgcgatgc cgtccgaacg cccccgcccc tttcagacgg
     8701 ggcatccgcc cgcaccaaac ctgcgggcgc ggacggcgat gcgcctgtcc ggcacttccc
     8761 aaatacttcc ctgtaaaata aggagtattt gaaaaatgaa gacattagaa aaacggatga
     8821 aagctctaga caaacggatg atgaagttag gaaaatccct tgaaggcagg cttgatgccc
     8881 gtctgattga atccgcattg gattatattc attattcgga acgttttttg gcttttgaaa
     8941 tcctgtgtac ttatattgaa gatttcgata tccggctgac ggaacaagaa tcccaagaaa
     9001 tttcttttat tgacaaggaa tttaggacag aaagtacgtt agattatcca ataaaatcaa
     9061 cgggatgata aacatgaaaa catcaacggt tgtttttggc ggatttttta ttacagataa
     9121 tggagaacga atccaaatcc ccgttttgga aaatcctggc attaaagaaa tcaatcactt
     9181 tttttccata tcaaattttg agaaaaaagc cggcgtcctt gttttcagaa tcatccccga
     9241 gccggaattt ggcaataccg aattaactat ctattttgaa aaaggatatt atttgccaat
     9301 gattcaaaca attttagaag gcggggatat tgaagtcaaa aaccttaaaa cggaaaatta
     9361 tagtggaaat acaatggaaa tttggggaga ttcctaccct attgagcata tctctaaaaa
     9421 tatttctaca attcaaaata taatttctga atttatgata caaaaacaaa caccggcacc
     9481 aataatttga tggaaaccca acaatcaaaa tgccgtctga agcatgtttg gttttcagac
     9541 ggcatttcct tcgcttaaaa cagcgtatcg gcaaccccgc cctgcccgtc cacggcaatc
     9601 tgcatctgaa aaccatctgt atcccaaacc acacccccat ccctgtttcc gtcatttgca
     9661 ccctgcccgt attgggcaat catcggtttt tcgcttacaa tagccgaatc tgaaccaact
     9721 ctctaaaaag gccgttccca tgcagtatgc aaaaatttcc ggcacgggca gctatcttcc
     9781 cgccaaccgc gtcagcaatg acgaccttgc ccaaaaggta gatacctctg acgagtggat
     9841 taccgcgcgc acgggcatca agttccgcca tattgcagcc gaaaatgaga aaaccagcga
     9901 tcttgccgcc gaagcagccc gccgcgcgct ggatgcagcc ggattagaca gcggcgaaat
     9961 cgatttgatt atcgtggcga cggcaacgcc ggatatgcag tttccttcta ccgccaccat
    10021 cgtgcagcaa aaattgggca tcaccaacgg ctgtcccgcg tttgacgtac aggcagtgtg
    10081 cgcaggcttt atgtacgcgc tgaccacggc aaacgcctac atcaaaagcg gcatggcaaa
    10141 aaacgcgctg gtcatcggcg cggaaacctt cagtcgcatt gtagactgga acgaccgcac
    10201 aacctgcgta ttgttcggcg acggcgcggg cgcggtggtt ttgagcgcgg cggacaaacc
    10261 gggcatcatc cacagcaaac tcaaagccga cggcaattat ctgaaactct taaacgtccc
    10321 cgggcaaatc gcctgcggca aagtttccgg ttcgccgtac atttcgatgg acggtcccgg
    10381 cgtgttcaag tttgccgtca aaatgctgtc caaaatcgcc gatgacgtta tcgaagaagc
    10441 cggttacacc gccgctcaaa tcgactggat cgtgccgcat caggcaaacc gccgcattat
    10501 cgaatcgacc gcgaaacatt tggggctgag tatggacaaa gtcgtcctga ccgtccaaga
    10561 ccacggcaac acgtccgccg cctcgattcc gctggctttg gatacaggca tccgcagcgg
    10621 acaaatcaaa cgcggtcaaa acctgctgct cgaaggcatc ggcggtggtt tcgcgtgggg
    10681 cgcggtgctg ttgcaatatt gaacccgatg ccgtctgaag caggttttca gacggcattt
    10741 cccatatcat gaagcggcag gctttcttca aaccaattgc gtgtgcggca tttctgtctg
    10801 ccgtttcgct gcgcctcccc gtattgggcg cgtgttacgc aatattgtcc ctctatgcgt
    10861 ttgcacttta cggcatcgac aaacggcgtg ccgtgcgggg aaaacgccgc attcccgaac
    10921 accgcctgct gctgcctgcc ttgttcggcg gttgggcggg cgcatacttg ggcagcagga
    10981 tattcaggca taaaacggcg aaaaagcgtt ttgttgtgct gttccgtctg actgtttcag
    11041 gtaatgtcct ggcgaccctc atcctgattt atagtggatt aaatttaaac cagtacggcg
    11101 ttgcctcgcc ttgccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt
    11161 tgttaatcca ctatattatt ttgtcccgcc tgaatttttc gtaaaactcg ggcagaatac
    11221 ctgattatcc aaccaaacaa aggaatacta tgtcttttgc cttctttttt cccggacaag
    11281 gttcccaaag cctcggtatg atgaacggct ttgccgaaca cgccatcgtc aaaaacacct
    11341 ttgccgaagc ctccgccata ttggggcagg acttgtgggc gatgattaac ggcagcgatg
    11401 ccgaaatcat cggacaaacc gtcaataccc agcccatcat gctcgccgcc ggcgttgccg
    11461 tttaccgcgc ctatttagaa gcgggcggca aaacgcctgc cgccgttgcc ggacacagcc
    11521 tcggcgaata caccgcactc gttgccgccg gcgcattgaa ttttgccgac gcggtcaaac
    11581 tcgtgcgcct gcgcgccgaa ctgatgcagt ccgccgtacc gcaaggcgtg ggcgcaatgg
    11641 cggcgattct cggcttggaa gatgagcagg ttaaagccat ttgcgccgaa gccgcccaag
    11701 gcgaagtggt cgaagccgtc aacttcaact cacccggaca aatcgtgatt gcaggcaacg
    11761 ccgccgccgt cgagcgcgcc atgaccgccg ccaaagaagc cggtgccaaa cgcgccctgc
    11821 cgctacccgt gtccgtacct tcccattgca gcctgatgaa acccgccgcc gaaaaacttg
    11881 ccgaagccct gaaatcggtt gtaatcaaac agccgcaaat ccgcgttatc cacaacgccg
    11941 acgttgccgc ctacgatgat gccgacaaaa tcaaagacgc gctcgtccgc cagctttaca
    12001 gccccgtacg ctggacggaa accgtcaacg cccttgtttc agacggcatc accgaatccg
    12061 ccgaatgcgg cccgggcaaa gttctggcag gtttggcaaa acgcatcaac aaagccgccg
    12121 cgtgcagcgc gctgaccgat gccggacaga ttgccgcctt tatcgaagca cactgacttc
    12181 gttccgcaaa aagcagcctg ccctcttcag gctgcttttc acgtccgaac ggcggcagcc
    12241 ccgtatttac gctataatcc atcccgacca aaccaccgac agcggctgcc gttgcagttc
    12301 ccgccctacc gatatgatag aaaaactgac tttcggactg tttaaaaaag aagacgcgcg
    12361 cagctttatg cgcctgatgg cgtacgtccg cccctacaaa atccgcatcg ttgccgccct
    12421 gattgccatt ttcggcgttg ccgccaccga aagctacctt gccgccttca tcgcccccct
    12481 gattaaccac ggcttttccg cacctgccgc gccgcccgag ctgtctgccg ccgccggcat
    12541 catttccacc ctgcaaaact ggcgcgaaca gtttacctat atggtttggg ggacggaaaa
    12601 caaaatctgg accgtcccgc tcttcctcat catcctcgtc gtcatccgtg gcatctgccg
    12661 ctttaccagc acctatctga tgacttgggt ctccgtgatg accatcagca aaatccgcaa
    12721 agatatgttt gccaaaatgc tgaccctttc ctcccgctac catcaggaaa cgccgtccgg
    12781 caccgtactg atgaatatgc tcaacctgac cgaacagtcg gtcagtaacg ccagcgacat
    12841 cttcaccgtc ctcacgcgcg acacgatgat cgttaccggc ctgaccatcg tcctgcttga
    12901 cctcaactgg cagctcagcc tcatcgtcgt cctgatgttc cccctgctct ccctgctctc
    12961 gcgctactac cgcgaccgtc tgaaacacgt catttccgac tcgcaaaaaa gcataggcac
    13021 gatgaacaac gtgattgccg aaacccatca gggacaccgc gtcgtcaagc tgttcaacgg
    13081 gcaggcgcag gcggcaaacc ggttcgacgc gatcaaccgc accatcgtcc gcctcagcaa
    13141 aaaaatcacg caggcaacgg cggcacattc cccgttcagc gaactaatcg cctcgatcgc
    13201 cctcgccgtc gtcatcttca tcgccctgtg gcaaagccaa aacggctaca ccaccatcgg
    13261 cgaatttatg gcattcatcg tcgcgatgct gcaaatgtac gcccccatca aaagccttgc
    13321 caacattagc atccctatgc agacgatgtt cctcgccgcc gacggcgtat gtgcatttct
    13381 cgacaccccg cccgaacagg acaaaggcac gctcgcaccg cagcgtgtcg aagggcgcat
    13441 cagcttccgc aacgtcgatg tcgaataccg ttcggacggc atcaaagccc tcgacggctt
    13501 caacctcgac atcagacaag gcgaacgcgt cgccctggtc ggacgttccg gcagcggcaa
    13561 atccaccgtc gtcaacctgc tgccccgctt tgtcgaaccg tctgccggca acatctgcat
    13621 agacggtatc gacatcgccg acatcaaact cgactgcctg cgcgcccaat tcgccctcgt
    13681 ctcccaagac gtattcctgt ttgacgacac cctgtttgaa aacgtccgct acagccgtcc
    13741 cgacgcgggc gaatccgaag tcctatccgc cctccaagcc gctaacctgc aaagcctgat
    13801 cgacgcatca ccgctcggac tgcaccagcc catcggatcg aacggcagca acttatccgg
    13861 cggacagcgg caacgcgtcg ccattgcccg cgccattttg aaagacgcgc cgatattatt
    13921 attggacgaa gccaccagcg cattagacaa cgaatccgaa cgcctcgtcc aacaagcgct
    13981 cgaacgcctg atggaaaacc gcaccggcat catcgtcgcc caccgcctga ccaccatcga
    14041 aggggccgac cgcatcatcg taatggacga cggcaaaatc atcgaacaag gcacacacga
    14101 acaactgatg tcccaaaacg gctactacac gatgttacgc aatatctcaa acaaagatgc
    14161 cgccgtccgg acggcataaa caaaatgccg tctgaaatgg tacaatcgct ccgacccctt
    14221 cagacggcat catatccgcc gacccatccg attatcttca atcaccgtaa aacccattat
    14281 gacccaagac aaaatcctca tcctcgactt cggttctcaa gttacccagc tgattgcccg
    14341 ccgcgtgcgc gaagcccacg tttactgcga actgcattcc ttcgatatgc ctttggacga
    14401 aatcaaagcc ttcaacccca aaggcatcat cctctccggc ggccccaatt ccgtttacga
    14461 atccgactat caagccgata ccggcatctt tgatttgggc attccggttt tgggcatctg
    14521 ctacggaatg cagtttatgg cgcaccactt gggcggcgaa gtgcagcccg gcaaccagcg
    14581 cgaattcggt tatgcgcaag ttaaaaccat agacagcgga ctgacacgcg gcattcaaga
    14641 cgacgcgccc aacacactcg acgtatggat gagccacggc gacaaagtgt ccaaactgcc
    14701 cgacggtttc gccgtcatcg gcgacactcc gtcctgcccg attgccatga tggaaaacac
    14761 cgaaaaacaa ttctacggca tccagttcca ccccgaagtt acccacacca aacaaggtcg
    14821 cgccctgttg aaccgctttg tcttggacat ctgcggcgcg caaccgggct ggacgatgcc
    14881 gaactacatc gaagaagccg ttgccaaaat ccgcgaacag gtcggcagcg acgaagtgat
    14941 tttaggtttg tccggcggcg tggactcttc cgtagcggcc gcgctgattc accgtgccat
    15001 cggcgaccaa ctgacctgcg tgttcgtcga tcacggtttg ttgcgcctga acgaaggcaa
    15061 aatggtgatg gatatgttcg cccgcaactt gggtgtgaaa gtgattcacg tcgatgccga
    15121 agggcagttt atggcgaaac tcgccggcgt gaccgacccc gagaaaaaac gcaaaatcat
    15181 cggcgcggaa tttatcgaag tatttgatgc cgaagagaaa aaactcacca acgccaaatg
    15241 gctggcgcaa ggcacgattt accctgacgt aatcgaatcc gcaggcgcga aaaccaaaaa
    15301 agcccacgcc atcaaatcgc accacaatgt cggcggcctg cctgaaaaca tgaagctcaa
    15361 gctgcttgag cctttgcgcg acttgtttaa agacgaagtg cgcgaactgg gcgttgcttt
    15421 gggtctgccg cgcgaaatgg tgtaccgcca tccgttcccc ggccccggtt tgggcgtgcg
    15481 catcttgggc gaagtgaaaa aagaatacgc cgacttattg cgtcaagcag acgatatttt
    15541 catccaagaa ttacgcaaca ccaccgacga aaacggcaca tcttggtacg acctgaccag
    15601 ccaggcattt gccgtattcc tgcctgtgaa atccgtcggc gtaatgggcg acggacgcac
    15661 ttacgactac gtcgtcgcac tgcgcgcagt catcaccagc gacttcatga ccgcacactg
    15721 ggcagaactg ccttactcac tgctcggacg cgtgtccaac cgcatcatca acgaagtcaa
    15781 aggcatcaac cgcgtggtgt acgatgtcag cggcaaaccg cctgccacca tcgagtggga
    15841 ataaccagca aacagggctg caccgtccgg cgcagtcttt cgattatcgg aaaaaaggaa
    15901 aaaatatgag cacacaagat ttaagcggca aaatcgcttt ggtaaccggc gcatcgcgcg
    15961 gtatcggtgc agcaattgcc gacacactgg cggcagccgg tgccaaagtc atcggtacgg
    16021 cgaccagtga gagcggtgcg gcggcgatta gcgagcggtt ggcgcaatgg ggcggcgaag
    16081 gccgcgtatt aaattccgcc gaacccgaaa ccgtcgaaaa cctgattgcc gacatcgaaa
    16141 aaacgttcgg caaactcgat attctggtca acaacgccgg catcacccgc gacaacctcc
    16201 tgatgcgcat gaaagaagaa gagtgggacg acatcatgca ggtcaacctc aaatccgtgt
    16261 tccgcgcttc taaagccgtc ttgcgcggca tgatgaagca gcgcgccggc cgtatcatca
    16321 acatcacatc cgtcgtcggc gtgatgggca atgccggtca aaccaactat gccgcggcaa
    16381 aagcaggctt aatcggtttc tccaaatcca tggcgcgcga agtcggcagc cggggcatta
    16441 ccgtcaactg cgtcgccccc ggctttatcg ataccgatat gacccgcgcc ctgccggaag
    16501 aaacccgcca agcctttacc gcccaaaccg ccttgggcag attcggcgac gcgcaagaca
    16561 ttgccgatgc ggttttgttc ctcgcttccg atcaggcgaa atacattacc ggccagacgc
    16621 tgcacgtcaa cggcggcatg ctgatgccct gacggacagc cgtgccgaca atgccgtctg
    16681 aagccctttc agacggcatt tgcattccca aacaaaatga acacacacca caccctgccc
    16741 tgcccctgcg gttcaggcac aagctgagac ctttgcaaaa ctcctttccc tcccgacagc
    16801 cgaaacccaa acacaggttt tcgtctattt tcgccccaaa tatctcctaa ttttacccaa
    16861 ataccccctt aatcctcccc ggatacccga taatcaagca tccgggctgc cttttaggcg
    16921 gcagcgggcg cacttagcct gttggcggct ttcaacaggt tcaaacacat cgccttcaga
    16981 tggctttgcg cactcactta atcagtccga aataggttgc ccgagcgtag cggaatttac
    17041 ggtg
//
LOCUS       DAXHTY010000038        16980 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXHTY010000038 DAXHTY010000000
VERSION     DAXHTY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16980
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.038"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(306..1718)
                     /gene="trkA"
                     /locus_tag="ACFNMK_001049"
     CDS             complement(306..1718)
                     /gene="trkA"
                     /locus_tag="ACFNMK_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC6364336.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAETGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(1816..3339)
                     /locus_tag="ACFNMK_001050"
     CDS             complement(1816..3339)
                     /locus_tag="ACFNMK_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFC6364337.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTSPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            complement(3646..4452)
                     /locus_tag="ACFNMK_001051"
     CDS             complement(3646..4452)
                     /locus_tag="ACFNMK_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6364338.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(4504..4755)
                     /locus_tag="ACFNMK_001052"
     CDS             complement(4504..4755)
                     /locus_tag="ACFNMK_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364339.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            4855..5616
                     /locus_tag="ACFNMK_001053"
     CDS             4855..5616
                     /locus_tag="ACFNMK_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364340.1"
                     /translation="MDVNKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRDHVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            5761..6930
                     /gene="metZ"
                     /locus_tag="ACFNMK_001054"
     CDS             5761..6930
                     /gene="metZ"
                     /locus_tag="ACFNMK_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC6364341.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VVSSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAMYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQVALIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAAGIRPGLVRLSVGLEYVGDLVDDLKQALAR"
     gene            7025..7231
                     /locus_tag="ACFNMK_001055"
     CDS             7025..7231
                     /locus_tag="ACFNMK_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC6364342.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(7329..7441)
                     /locus_tag="ACFNMK_001056"
                     /pseudo
     CDS             complement(7329..7441)
                     /locus_tag="ACFNMK_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(8278..9030)
                     /locus_tag="ACFNMK_001057"
     CDS             complement(8278..9030)
                     /locus_tag="ACFNMK_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6364343.1"
                     /translation="MTDILIDNTATEAVRALIQAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAVLLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
     gene            complement(9134..9601)
                     /locus_tag="ACFNMK_001058"
     CDS             complement(9134..9601)
                     /locus_tag="ACFNMK_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFC6364344.1"
                     /translation="MTELQHPVLVVDNETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            complement(9594..11170)
                     /locus_tag="ACFNMK_001059"
                     /pseudo
     CDS             complement(9594..11170)
                     /locus_tag="ACFNMK_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
     gene            complement(11191..13494)
                     /gene="parC"
                     /locus_tag="ACFNMK_001060"
     CDS             complement(11191..13494)
                     /gene="parC"
                     /locus_tag="ACFNMK_001060"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC6364345.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPISSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SSGYGFITKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSELEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGRLLPISGSLKQLSSPK"
     gene            13791..14474
                     /locus_tag="ACFNMK_001061"
     CDS             13791..14474
                     /locus_tag="ACFNMK_001061"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC6364346.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDKDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            complement(15043..>16980)
                     /locus_tag="ACFNMK_001062"
     CDS             complement(15043..>16980)
                     /locus_tag="ACFNMK_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC6364347.1"
                     /translation="ISPEIFGTLFQSVMDAQERREAGAHYTETANIDKVINGLFLENL
                     RAEFEAVKAFKRDKAKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAE
                     ALKDKAGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEV
                     ACHTLPLEDAAEIIHANSLRTPWQAVDYIFGNPPFIGSTYQTKEQKNDLESICGHIKG
                     YGLLDYVCNWYVKAAGIMAQHPQVQTAFVSTNSICQGQQVEILWGSLLNQGIEIHFAH
                     RTFQWTSQAAGKAAVHCIIVGFRQKPPMPSEKTLYDYPDIKGEPVKHAAANINPYLID
                     APDLIIAKRRTQISGEIEMLYGNKPTDGGNLILSTAEKDALIAAEPLAEQYTRPFVGA
                     EEFINGKTRWCLWFHGVSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATP
                     WLFQKIRQPSDGNFLIIPRVSSESRRFVPIGYLSYETVVSDLVFTLPNASLYHFGILS
                     STMHNAFMRTVAGRLESRYRYSNTVVYNNFPFPESCRMPSENDRQDPLRSAVETAAQA
                     VLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALDKAVDKAYGYKTGKNT
                     DDEAERTAFLFELYRKAAAIA"
BASE COUNT         3514 a         4144 c         5094 g         4228 t
ORIGIN      
        1 tctttgaata ttgctgttgc ccaatggttt ggattcccgc aacctttcgt cattcccgtg
       61 gaaacgggaa tctagaatct ctaaagcttc agctaacctt tgaatattgc cgctatccca
      121 aggtctggat tcccgcctgc gcgggaatga cgggtctttt ataacctttg aatattgccg
      181 ctatcccaag gtctggattc ccgcttacgc gggaatgacg gggagggcgg atgccgtctg
      241 aagcccacct accattattt ctgcttccat accgcttggc ggcaatatgt ccggaatggg
      301 gcggtttatc cgaaaaagcc cattttgacc tggatgagtt tttccagttc gttcaggatg
      361 cgccggcgcg agacgaaaaa gatgatgtgg tcgccgtctt ggatgacggt ttcggtatgg
      421 tgtcccataa tggtttcgcc ggtttcggcg cggacgacgg cggcaatgtg gcagccttct
      481 ggccatttga taccgctgat gcgcctgccg atgatggcgg aagttttttt gtcgccgtgt
      541 gcgacgactt cgatggcttc cgccgtgccg cgccggatgg ggtggacggc aacgatgtcg
      601 ccgcgccgga tgtgggcgag tatcgagccg atggtgatga ggtgggggga gacgacgatg
      661 tcgattttgt tgccttcgag caaatcgacg tagcttgagc ggttgacgat gccgatgacg
      721 cgcttcgcgc cgaggttttt cgccaaaagg gcggacataa tgttgctttc gtcgtcgttg
      781 gtcagggcgc agaatacgtc gatttcgtcg atgtattcgt tgtcgagcag ggtttcgtcg
      841 gttgccgaac cttgcaggac gagggtgttg tcgaggtttt cggctatcca ttcggcacgg
      901 cgcggccggc attcgatgat tttgacgttg tatgcgtgtt cgagctgctt ggcgagacgg
      961 tagccgatgt tgccgccgcc ggcaatcatg atgcggcggg tgctggtttc tttggggcgc
     1021 aattcgggta tgaccgcgcc gatgttttcg gcggcggcgg caaacaggat ttcgtcgcct
     1081 tcgatgatga cggtttgcgg cgctgggacg atgaggcggt tgttgcggta aacggcgcag
     1141 atttggcagt cggccccgtc gggcaaatct tgggcgatgt cggcaatgct gcgtccgaca
     1201 agcagtccgc cgcgccgcgc ctgtatgatg accatccgca cgcggtcgtc tgcaaaacgt
     1261 aaaacctgca atgcgcccgg gcagtctatc aggccggcaa gctgttcggt aacgagctgt
     1321 tcggggctga tggtttcggt tatgccgaat atggaaaggc tgccgttttc gttgttttcg
     1381 agcttggggc tgaggtattc gaggtattcg ctggaacgga cgcgcgcgat gcggccgggg
     1441 atgttgaaca ggtcggcggc aactttgcag gcgacgatgt tggtttcgtc gctgcgggag
     1501 agcgcgagca gcaagtcggc atcttccgcg ccggcgcgtt ctaatgtgaa gggggatgcg
     1561 ccgttgccga aaacggtttg gacatcgagg cggctgcctg tttcctgcaa tgctttttcg
     1621 tcgatgtcga taacggttac gtcgttgttg ggtatggcgg caaggttttg tgcgacggta
     1681 gaacctacct gtccgttgcc taaaatgagg attttcacgg tgtgggtcgc cgagtgatga
     1741 aatggtggat tgtaccgcaa ccgggggcgg aaatgccgtc tgaaggcaga ttttgtgttc
     1801 agacggcatg gcgcctcaag attcgacggg gatgatgccg attttcgcct gccatttgcg
     1861 cggggcggtg gcgtggatgg attcgccttt gctatctacg gcgacggtta cgggcatgtc
     1921 tttgacttca aattcgtaaa tggcttccat gcccaattcg gggaacgcca agactttgga
     1981 agatttgatg gcttttgcca cgagatacgc cgcgccgccg actgccatga ggtacacggc
     2041 tttgttgtcg gcgatggctt cgcaggtggc cacgccgcgc tcggatttgc cgatcatgcc
     2101 caagaggtcg gtttgttcga gcatttggcg ggtgaatttg tccatgcggg tggctgtggt
     2161 cggacctgcc ggaccgacga cttcatcgcc gaccggatcg acgggaccga cgtagtaaat
     2221 caggcggttg gtgaaatcga cgggcaattc ttcgcctttg ttgagcatat cgacgaggcg
     2281 tttgtgtgcg gcatcgcggc cggtgaggat tttgccgttc aacagcaata cgtcgccggt
     2341 tttccagctt gccacttctt ctttggtcag cttgtcgaca tcgacgcgtt tgccgttgtc
     2401 ggggctgtaa gtcaaatcgg gccagtcttc gacgcgcggc gatgtgagtt cgacagggcc
     2461 tgagccgtcc aattcgaact cgacgtggcg ggtggcggcg cagttcggaa tcatggcaat
     2521 cggtttggag gcggcatggg tcggataatc gaggattttc acgtccaaca cggtggtcag
     2581 tccgcccaag ccttgtgcgc cgatgcccag cgcgttgact ttttcaaaga gttcgaggcg
     2641 cagggcttcg gtggtggaca attctgcgcc ggacgcggct ttttcctgca attcttggat
     2701 gtcgatgtgg ctcatcaggg actcttttgc catcaacacg gctttttcag gcgtgccgcc
     2761 gatgccgatg cccaagatgc cgggaggaca ccagcccgcg cccatcgtcg gaatggtttt
     2821 caatacccaa tcgacgatgt tgtcggaagg gttgagcatg gcgagtttgg atttgttttc
     2881 agaaccgccg ccttttgccg cgcaggttac ttcgactttg tcgcccggca cgatgctcat
     2941 atgaatgacg gcgggggtgt tgtctttggt gttttgacgt ttgccggccg gatcggcgag
     3001 gacagaggcg cgcagggtgt tgccttccca agtgtaggcg cggcgcacgc cttcgttaac
     3061 catttcttcc acgctcatgt ccgcatccca ctgcacatcc ataccgactt tcaaaaacac
     3121 ggttgcgata cctgtgtctt ggcagatggg gcggttgttt tcggcacaca tgcggctgtt
     3181 gaccaaaatc tgcgtcatcg cgtctttggc ggcggggttt tcttccttct gccacgcttt
     3241 atagagcgcg tcgatgtagt ctttgggatg gtagtagctg atgaattgga aggcatcgca
     3301 aatgctttgg ataaagtctt cctgtttgat gacggtcatg attgtgttcc ttttcaaggt
     3361 tgtggcggta agtgtctgct gctgttttaa acaacctttt tgaaagctgt tttttatcag
     3421 gttgaaatag tgccagcgtt tgtacggtaa agacaataca taattctaat ctcttgatta
     3481 tcaatttgtt ttaataaaat actgttttac tgggattttg atatttgtaa tctttgcctg
     3541 ttgctttgcg gtagggagaa atcagcaggc agttcattgt aggccgattg atgaacaatg
     3601 ccgtctgaaa gggcttcaga cggcattgtg ttcggacggg cgggcttatt ttgcggcctg
     3661 tccgtcttct tttgcaaaat atttggcgta aatcttgtcg tattcgccgc tttcgcgtac
     3721 tttttccaac gcatcgttca gcattttgac ggttgcttcg tcgcctttgc gtaccgcgat
     3781 gccgtagtgt tcggtggtga agtcgggcag ggtaacgaag tccattcctt tggtcggatt
     3841 gtttttcaca taattggcga tgaccgcgct gtcgctgact acggaatcta agccgccgtt
     3901 ttccagttct ttgataatca ggggaacgtt ttcaaagcgc gcgattttcg ggttgtcgtt
     3961 gcccaagagt ttggatacgg agaaatcgcc cgtgtagccg gttaccacgc cgactttgtt
     4021 catgtttttc aaatcttcgg aagaagatat ttttttgcct ttcggaacga ggacgacttg
     4081 ggtgatttca aaatacgggt cgctgaagtc catagactgt ttgcggtcgt cggtaatggt
     4141 tacgcccgac atcacaacgt ccgcatcgcc gttgttcaag gcggggaaaa ggctgtccca
     4201 cggctggtgt ttgaattcga ttttaaaatt gcccgccttc gccatcgcgt tcatcaaatc
     4261 cacatcgaaa ccttcgacat tgcctttcga gtctaaagat tcaaaggggg caaactcggc
     4321 gttggaagcc acgcggtaca ctttgtcggg gtttgcggcg ggcgcggcgg catctttacc
     4381 ctgaccgccg caggcagaca gcgcgagcgc ggaacaggca agggcggcgg caatccattt
     4441 tttcatattc atcaaaattc ctttcggtaa tcaaaaagcc taaaaaatca gtttaacata
     4501 aaattaacgt tctttttcac ggaaaaacgc ataagtaacg tcgtggaaca aatcggtatg
     4561 ggggctgtcc tcatagctca attctgccaa ggcgtcctca aaagcaagct gaatcgactc
     4621 gaccgcgatt tcggcggttt cggcgggaag ggcgcggatt aaggctttga atgcggtgga
     4681 caggacgtgg ttctgcatac gcagcacgtc gttcgcctct tccagatatt ccaaacgctg
     4741 ttcgatgctg cccatgatgt atcctcgcga atctgttaaa atgcgccatt atatcaaacc
     4801 gcgctttggg cgcgggtttg tctgcctgca aagattaaga accggaagcc gaaaatggat
     4861 gtgaataaag agccgaattg ggaaaaacgt ttggaaaacc tgcgtcagac gcgccgtatg
     4921 aaggcagtgc cgacttacgc gccgctgtcc cgcccctctg aaaccggaac ggacgcgcct
     4981 gtgccgaaac tggacgatga gagccgagac catgtgttga aggcgtatct gaaaaaatgg
     5041 caggaggaac acaatgccgc cgttgccgat tgcggcgagg aagtcgaaac cgacccgatg
     5101 attgtccttc aggaaaactg gctcaacgcg caggcttccc tgcaaatgtc ggtttcggaa
     5161 aaacacatcg aatcgcggcg gagaatcctg ttcgatgcca aaaaagacag tgccgaattt
     5221 gaaaccggac agattgaagg cggggaaaac gccgatgcgg cagaatcggg tacggcggca
     5281 gacggggttg cggaaaacgg cgaaggcggt gcggaagagg cgttgatgcc cgtccaaatc
     5341 aatatcctga acccgaaagc cgtcaaccgg cgcgaagtgt tctgcctgtc cgaacaggag
     5401 ctgaccgaac ggctgatcaa acgcctgcgc ccccatctga ccgataccgt caacggcatc
     5461 atccgtacgg cggtgcagaa gcagatggcg gtttttacct accagctcca gcagatgctg
     5521 cacgagcagg cgggggcggt agtcgaagac gtgttggaac acgatgtccg caaaatcctc
     5581 aacgacatca agtacgagtt aaaatacaaa cgctgaccgt catgccgtct gaaagccgtt
     5641 tcagacggca ttttcctgcc cgtccgactt gaaaggcgca cggaacgggc gcaaaatatt
     5701 aacaaattaa acaacgggcg cgcgccccgt ttttgaacgg acaaaaccga ggaatcaccg
     5761 atgagcaaaa aacttcatcc gcaaacgctc gccatacgcg gcggcaaaga acaaaccgga
     5821 taccgcgaac acaatcaggc cttgttcctg accagcagct tcatgtggga caacgcccaa
     5881 cacgccgccg atttgttttc aaaaaaaatc aaaggcttca cttatacccg taccgccaac
     5941 ccgaccacag ccgcctttga aaaacgcatc gccgctttgg aaggcgcgga acgcgcggtc
     6001 gccacttcga cgggtatgtc tgcgattcag gcggcgtttt tcaccttttt gcaggcgggc
     6061 gatcatgtgg tttccagccg cagcctgttc ggcacgaccg tcggctttat caataacatc
     6121 gttaccaaat tcggcatcga agtgagccac gtgtcgccga ccgatataaa cgaatggaaa
     6181 gccgccgtca aagctaatac caaactgctg tttttggaaa cgccgtccaa ccccttgggc
     6241 gaagtggccg acttggaagc tttggcggaa ttggcgcacg gcatcggcgc gctcttggtg
     6301 gtggacaaca gcctcttgtc tccagtcggc tctcagcctt tgaaacacgg tgcggatatt
     6361 tccgtttcct ccgcaaccaa agccatagac ggacacgggc gcgtgatggg cggcgtgttg
     6421 gcgggttcgg aagaactgat ggcgcaggtt gccatgtatt gcaactcttg cgggctggca
     6481 atgtcgccgt ttaatgcatg gcagttgttg agcggcgtag aaaccctgtc gctgcgtatg
     6541 gaaaaacagt tcgacaacgc cctgaaaatc gcgcaatggc tgcaagcgca gccgcaggtt
     6601 caagccgtgt attacaccgg attgcccgac catccgcagg tggcgcttat ccgcaaacag
     6661 caaaacggcg gcggcatcgt catcggcttt gaagttgccg accaagaagc ggcgtggaaa
     6721 gttgtggacg gcgtggaact cttttcccgc accgccaacc tcggcgacgt gcgctcaacc
     6781 attacccacc cgtggacaac cacgcatggc agaatgcagc ccgaagaaaa actcgccgcc
     6841 ggtatccgcc ccggattggt gcgcctgtcg gtcggtttgg aatatgttgg cgacctcgtc
     6901 gatgatttga aacaggcact cgcacgctga ttgcgtgttt cagacggcat cggcggaaaa
     6961 acaccgcaaa tgccgtctga aacccttatc ttatatggca tacacatcaa caatagggaa
     7021 aaatatggat gaagcggttt ttgccgattg ggcgttgaaa atctgcctga ccggtttgat
     7081 tatttttttg ggttttattg tttggaattt gggcaaggag tccaaagcag gcaaattcgg
     7141 catcgccatt ttgtttttgg ttttgggctt gggcgtgttc ggttttgtgt tcaaagagtt
     7201 attgattaag tttttggtat tgccgaaata ggctgctgcc tgtcaaaatg ctgtctgaaa
     7261 tcataaaaag taatctgcac cctaaaagtt tgatttaacc gccaactgat taaggtgcgg
     7321 atttttatga caaaatatag tggattaaca aaaaccagta cggcgttgcc tcgccttgcc
     7381 gtactatttg tactgtctgc ggcttcgtcg ccttgtcttg atttaaattt aatccactat
     7441 aaaaatcatc tttccgcagt cattcccacg gatgcgaaag cctgtctttt tgaatcccgg
     7501 ataattttac atcgtcattc ccgcgcaggc gggaatccaa atcattgggt agcggcaatg
     7561 ttcaaaagtc gtctgaaaaa tcagaagttc tagattcccg ttttcacggg aatgacggaa
     7621 tttcagacgg catcctcccg ccccgtcatt cccgcgcagg cgggaatcta gtccgttcgg
     7681 tttcggtcat ttacgataaa ttcctgttgc ttttcatttc tggattccca cttccgtggg
     7741 aatgacggag gagcggataa attctcgcaa tctaaaacct tgtcatttcc ataaaaaaca
     7801 gcaacccgaa acagcaactt aaaaacagca acctgaaacc tcgtcattcc cgcgcaggtg
     7861 ggaatccaga tcattgggtg gcggcaatgt tcaaaagtcg tctgaaaaat cagaagttct
     7921 agattcccgt tttcacggga atgacggaat ttcagacggc atctgcccgc cccgtcattc
     7981 ccgcgcaggc gggaatctag tccgttcggt ttcagtcatt tacgataaat tcctgttgcc
     8041 tttcatttct agattcccac ttccgtggga atgacggttt ggaagttgcc tgaaactcga
     8101 aaacaactat agtggtttaa atttaaacca ctataaaact gaatgaatcg gattcccact
     8161 tctgtgggaa tgacaaactt taaggtgtga tgacttatcc aaaacagtcg aaacgcaaaa
     8221 accggtttct cgtttgcata agaacggcaa atttttcggt gtcttgtttt atgggcgtta
     8281 tcccttgtcc gtcccgtagg gcaggtaaac gtcgaagcgg gtgcttttgc cggtgtattg
     8341 gtaggcaggg gcttgtccgg caaggtgttc gccgaggcgg gggcgtttga cgacgacgcg
     8401 tttttttgca gtctgtcggg cggtatggag gaggacggcc tcatcttgcg cctcgccgac
     8461 gagccggtgg aagtaggtca tttctttttt aacggcggca cttttgcggc gttcgggata
     8521 catggggtcg agatagacga tgtcgggttt gccttgtgtt tgaacaaggg cgggcatttg
     8581 tactgcggcg ttgccgaaat gcagggtgat gcgcgcggcg gtgtcttgcg tttcgggatt
     8641 gaggagggcg cggcggatgc cgtctgaaag caggcaggct acggcgggat gctgctcgaa
     8701 ggcggtaacg gccagcccga gcgaggcgag gacgaagctg tcgcgcccca atcctgcggt
     8761 tgcgtcccaa acggtggggt gcgcggtgtg gttgacggct ttggcgatga gttcgccccc
     8821 gccttttgtg cgccggtatt gtgccgcgcc ggaggcaaaa tcgacgatga cgctgctttt
     8881 ttccccgaca agcctgaggc tgacggtatc gtgttcggca aggaggtaac tgccttgttc
     8941 gggcggttgg gaaacgggca caagggggaa tgcctgtatc agggcgcgga cggcttcggt
     9001 ggcggtgttg tcaatgagga tgtcggtcat ggtgttttgt gtccgttcgg gcgtggggcg
     9061 aacccgcttt gccgtgccgt ttcagacggc attttttcgg gtgggcggag atggtgcgga
     9121 tgccgttttg tcgtcagccg atgttgaggc gttccatacg gtagcgcatg gaacggaagc
     9181 tgatgcccaa gcgtttggcg gcctgcgtgc ggttgccttc ggtttgtttg agggtttgtt
     9241 cgatgatgtc gcgttcgatt tggtcgagat agtcctgtat ctgcatggta tcggggtcga
     9301 acgcgccgca acgcaacgct tcctgtgcct gatcggcgtt gccgaaggcg gtgatgacgg
     9361 caaccggcgt atcgagcagg cggctgttga tgtgttggac gacttccagc cccgagccgt
     9421 ccggcatacg catatcggtc aggacgagcg aatagcgttg gctgtcgagc ttgtttttgg
     9481 cttcggcaac gcctgacgcg gtatggacgc gcaagcccat tttcatcagg gtcatttcca
     9541 tcaggtcgag aatgtcggtt tcgttatcga cgacgaggac ggggtgttgc agttcagtca
     9601 ttggtttttt ccggtaatgc gagttcgaaa catttggctt cgggcaggta ggtcaaatcg
     9661 ccgaagttgg catgtgccag ttcgcgggcg acatacagcc cgaggccggt gccgttttcc
     9721 gccgtggtgt aaaagggttc gaacaggtgt tcctgcactt cgggcggtcg gcaaagagga
     9781 tgcagacggt gtttttttgc gcggggcgga tggtgacgga aatcgagccg ggctgtttgc
     9841 ggctgtgccg ccacgcgttg ttggcgaggt tccacataat ttgccgcagg tgggcgggat
     9901 cgaaataggc ggtcgggtga ttgccctgca tatccaagcg gatgcagccg gcggcatcgg
     9961 gatggccgag caggaactct tgtttgaatt cttcccaaaa cggtatcagg ccgatggttt
    10021 cgcgttcggt tttgttgcgt ttgttgagcg aggagatgtc ttcgagcatt ttgtcgatac
    10081 ggcggacgtt gccgtcgatg atttcgcaaa gtttgacctt gaacgggtcg gtttcttctt
    10141 cctctgcatt ttcgcgcaac aggtcgtttg catggcgaat ggcggacatg gggttgcgga
    10201 tttcgtgggc gatgttggcg gtcagttgtc cgagcgcggc aagtttgacg gacagggctt
    10261 ctgcctgaat ttcgctttgc gggcggatgt agaggatgag cagcttgttc tgctctttgt
    10321 tcatcggcac ggcgcggatg cgggcggtca gggcgggcgt gtcgatattg cgttcgaaag
    10381 tacgcgaagg ggccttgtcc cataaaacgg taatagggtc gaacagggcg gtatgctgtc
    10441 cgatttcaag cgtggggagc agatcttttg cctttttatt gaacagtacg gtctgatact
    10501 cgacgttgat gatgacgacc gcttcctgaa cgcggttgag cacgatttgg ttcaagcccc
    10561 tgatgcggcg gtaggcggcg tggttttcat gggcaagttt gccggcacgg tcgatgtatc
    10621 tgaccgacag cgaggcaatc atggcgacga aataggagcc ggtcacgagg acgaaggtgt
    10681 tggtgacggt ttttgcatcc aaaatcagcg gatacatatt gatattgctg tctgcaaggg
    10741 cattcaatat cagcaggatg gcggcgtagc tggcatagag cagggggtaa cgcccgtaac
    10801 tgagcaggca ggagctgccc acgaagggca ggatcaggat gccgaacccc gagttgatgc
    10861 cgccgaacag gtaggtcagt atgccaatca tggtaatgtc ggacacggcg ctgaaactcg
    10921 gtatcttcaa agcctgccat tgccattgcg gattgagggt ggaaaagaaa atcatccagc
    10981 aggaaatggc gagccacaag taaaatgccc atgtcgtcca tgagacgtgc aggttttcat
    11041 gcccgctgac ctccaacccg aagatatgca taatcagcag ggggaggacg gtggcgatgc
    11101 gggcgatgtt gatcagattg ggaatctggt ctttgagttt ctcaagttcg cgggggttgg
    11161 aaatcaccat aattatgtgt ggaatcgggt ttatttcggg gaagaaagct gtttcagact
    11221 gcccgatatg ggcaatagtc tgcctttttt gccgcgtttt gcctcaagca gggagatggg
    11281 gatgcggtct ttgtgcgccg cgccgcgcct gccttcgctt tcaatcagga tttccagctc
    11341 ggaagaaacg gcggtatgcg tcatcgattc gccggcgttt aatccgatga tttgcagccc
    11401 tttgcctttc gccataattt tcaattcgcc gatggggaag gcgagggcgc ggttttgact
    11461 ggtggcggca atgattttgc agtcggggtt gatgaacgag gaggcataga cggcaaccgg
    11521 cggcaaaacg gtttcgccgc tgtctgcggt catcaccact ttgcccgctt tcacgcgccc
    11581 gaccatatcg cccagcttgg tgatgaagcc atagccgctg ctgcttgata ataaataatg
    11641 ttgttccggc aatcctgtca acatcgcaac gggtttcgcg ccgttttgca gctcgatcaa
    11701 ggatgaaatc ggtacgccgt cgccgcgtcc tccagggatt tcggcggcat cgagcgtgta
    11761 ggttctgccc agtgaatcca agatgacgac gggtaaaacg gtgcggcctt cgagggtttg
    11821 tttgaggcgg tcgccttctt taaacgcagt ttggctcaaa tcaaggttgt gtccggcacg
    11881 gctgcgtatc cagccttttt ccgacagaat cagcgtgatc ggctcatcgg cggtggtttg
    11941 cgtcaacacg gcgcgtccgg cttcttccac cagcgtgcgg cgcgcgtcgc cgaactgctt
    12001 catgtccgcc tgcatctctt tgataatcag cttgcgtttt tcgttttcgt cgcccaaaag
    12061 gatgttcaga cggccttgtt cctcgcgcaa ttcgttcaat tctttttcga gtttgaaacc
    12121 ttccaaacgc gccaactggc gcaggcggat ttccaaaatg tcttcggctt ggatttcggt
    12181 cagcccgaac gccgccatca aatcggcttt cgggtcgtct gattcgcgga tgactttaat
    12241 cacttcgtcg atgtgcagaa agactttcag acggccttcg aggatgtgca gccgtttttc
    12301 cacttggttc aaacggaatt tcagacggcg tgttacggtt acggtgcgga aatccagcca
    12361 ttcctgcaaa atcgttttca ggtttttctg cgcggggcgg ttgtccagcc ccatcatcac
    12421 caagttcatg gacacattgc cttccagcga agtttgcgcc atcagcgtgt tgatgaaggt
    12481 atcggtatcg atgcggctgg atttcggttc gaacaccagt cgcacgggat gttcgccgtc
    12541 ggactcgtcg cgcacgcggt cgattaaatc cagcatcagc tttttggtat tgagctggtc
    12601 ttggttgagc tgctttttgc ccgctttcgg tttcgggttg gtctgctctt cgatttcggc
    12661 aaggattttg gcggaattgg cgttcggcgg tagctcggtt acgatgacgc gccattgtcc
    12721 gcgcgccaat ttttcgattt cataacgcgc acgcacgcgc acgctgccct tgccggtttc
    12781 ataaatccgg cgcaattcgt ccgccggcgt gatgatttga ccgccgccgg caaaatcagg
    12841 tgcgggaatg tattgcatca ggtcggcggt ttccagcgtc ggctttttca acagcgcaat
    12901 cgccgcctgc gtaacttcgt tcaaattgtg cgacggaatc tcggtcgcca tgcccaccgc
    12961 gatgcctgac gcgccgttga gcaacaccat aggcaggcgg gcgggcaggt gcagcggctc
    13021 gtcaaacgcg ccgtcgtagt tcggcacgaa atccaccgtt ccctgattga tttcggacaa
    13081 cagcaattcc gcaatcggcg tgagccgcgc ttcggtgtaa cgcatcgccg ccgcaccgtc
    13141 gccgtcgcgc gaaccgaagt tgccgatgcc gtcgattaag ggatagcgca aggtaaaatc
    13201 ctgcgccatc cgcaccatcg cctcataggc ggaactgtcg ccgtgcggat ggtatttacc
    13261 caaaatctca ccgaccacgc gtgccgattt caccggcttc gcccccgccg tcaaacccat
    13321 atcgcgcatc gcaaacaaaa tgcgccgctg aacgggcttt tggccgtctg aaacttcagg
    13381 cagcgcgcgg cctttgacca cgctcatggc gtattcgaga taggcgcgtt cggcgtatcg
    13441 gccgagcatc agcgtattgg aatcggtatg tggagcgtgc ggttgcatat ttatcgtatt
    13501 tccttttcgg ttgaaccccc gccacttgga catctgtcct tcggggcggt agaatcagac
    13561 tttatttggg agggggcaac cccttccaaa tcagggcaac acatagggcg gtgctttatg
    13621 tgtcgtcctg tgtgttgaaa cattgtgtat gcggaatgta aagcaaaggg cattattgta
    13681 aatcaaaacg gcaaaccata gtgggcgcgt gtctgaatct gaatcaaata cgggaaatgt
    13741 gaaaatatgt tataaataag gctttcccac tttcacacat tggagacgat atggaactgg
    13801 tatttatccg ccacggacaa agcgaatgga acgcgaaaaa cctgtttaca ggctggcgcg
    13861 acgtgaagct gagcgagcag gggcttgccg aggctgccgc cgccggtaaa aaactgaaag
    13921 aaaacggcta tgagttcgac atcgccttca catccgtcct gacccgcgcg attaagacct
    13981 gcaacatcgt tttggaagaa tccgaccaac tgttcgtacc gcaaatcaaa acatggcggc
    14041 tgaacgaacg ccactacggc caactgcaag gtttggacaa aaaacaaacc gccgaacaat
    14101 acggcgacga gcaagtccgc atctggcggc gcagctacga caccctgccg ccgcttttgg
    14161 acaaagacga tgagttttcc gcacacaaag accgccgcta tgcccatctg cctgccgatg
    14221 tcgtacccga cggcgaaaac ctgcaagtaa cgctggagcg cgtattgccg ttttgggaag
    14281 accaaatcgc ccccgcgatt ttgagcggca aacgcgtctt ggtggcggcg cacggcaact
    14341 ccctgcgcgc gctggcaaaa cacatcgagg gcatttccga caaagacatc atgggcttgg
    14401 aaatcccgac tggccagccg ttggtgtaca aattggacga caatttgaaa gtcatcgaaa
    14461 aattctacct gtaagggtgt gaaataaaaa atgccgtctg aaggcttgag cgttcagacg
    14521 gcatttttga cggtaaacgt agcaactgct ttcgcgagaa cgacgggatt ttaggttttt
    14581 gtttttctgt tttcgtggga aggatgggtt ttaggttgcg gtcatttatc ggaaaagcag
    14641 aaaccgctcc gccgtcattc ccgcgaaggc gggaatctga acacgtccgt agggaaacct
    14701 atatcccgtc attcccatga aagtgggaat ccaggatgca ggggaaatcg ttttatccga
    14761 taagtttccg ccccgaaagg tctggattcc cgcctgcgcg ggaatgacgg gatttttgta
    14821 accgcagggc gggcttcagc ctgccaatca acaaccgaaa atgaagaaaa acggtgggct
    14881 gaagcccacc ctacgcaatt gcttttgcgg agatgacggg gttttagggt gaagatggtg
    14941 ggagttggtg ggagttggtg ggctgaagcc caccctacgc aactatccta cgcaactact
    15001 acgcaactac tacgcaacta ctacgcaact actacgcaac tactacgcaa ttgccgccgc
    15061 cttgcggtac agctcgaaca ggaaggcggt gcgttcggct tcgtcgtcgg tgtttttgcc
    15121 tgttttgtag ccgtaggctt tatcgactgc cttgtcgagg gcggcgtggg ctttgtctag
    15181 ggcggtatag cctgcgtcgg gcgcatagag ttcggcgagg gtcggctcgg gcaaaccggc
    15241 ttcccgcgct tctcggcggt attgtccgcg cgcgtcgagg acggcttggg cggcggtttc
    15301 gacggcggag cggagcgggt cttggcggtc gttttcagac ggcatccggc agctttcggg
    15361 gaaggggaag ttgttgtaca caacggtatt ggaatagcgg taacgacttt ccagacggcc
    15421 ggcaacggta cgcataaagg cgttgtgcat ggtggagctg aggatgccga agtggtagag
    15481 gctggcgttt ggaagggtaa ataccaaatc gctgacaact gtttcatatg atagataacc
    15541 gatggggacg aaacggcggc tttcggatga gacgcgtggg ataatcagaa aattgccgtc
    15601 tgaaggctgg cggatttttt gaaaaagcca cggggttgct gcatcttttt gagtttgttt
    15661 gtcgctgctg gcttcgcgca tggttttgac tgcctgaata cgggcttgaa cttgtggcat
    15721 ttgtttcagg tcgtggttgc gtttgacatc ggatacgccg tgaaaccaca ggcaccaacg
    15781 ggttttgccg ttgataaatt cctctgcgcc gacaaagggg cgggtgtatt gttccgccaa
    15841 gggttcggcg gcaatcaggg cgtctttttc ggcggttgag aggataaggt tgccgccgtc
    15901 ggtcggctta ttaccataaa gcatttcaat ttccccgctg atttgggttc tgcgcttggc
    15961 gataatcaaa tcgggcgcgt caatcagata cggattgatg ttggccgcgg cgtgttttac
    16021 gggttcgcct ttgatgtcgg gatagtcgta gagggttttt tcagacggca ttggcggctt
    16081 ttggcggaag ccgacgatga tgcagtggac ggcggctttg cctgcggctt ggctcgtcca
    16141 ttggaaggtg cggtgggcaa agtggatttc gatgccttgg tttaaaaggc tgccccagag
    16201 gatttcgacc tgctggcctt ggcagatgga attggtggaa acaaatgccg tctgaacttg
    16261 gggatgctgc gccatgatgc cggcggcttt gacgtaccag ttgcagacgt aatccaacag
    16321 gccgtagcct ttgatatggc cgcagatgct ttcgaggtcg tttttctgct ctttggtttg
    16381 gtaggtcgag ccgataaagg ggggattgcc gaagatgtag tccaccgcct gccaaggtgt
    16441 gcggaggctg ttggcgtgga tgatttcggc ggcgtcttcg agcggcagcg tatggcaggc
    16501 gacttcgccg tcgaagcgga tttgcgtgcg gatgttgcat tggtggtttt tgagccacat
    16561 ggcggtgcgg gcgatgagga cggcaaattc gtctatttcg atgccgtgaa actgtttcag
    16621 acggcattgg acggacgggc tgtcgaacag gccgcctgct ttgtctttga gggcttcggc
    16681 gatgatgtcg tcttcaaggg cgcggatgcg gtcgtaggcg acgataagga agttgccgca
    16741 gccgcaggcc gggtcgagga attgcaggtt ttggattttt tggtagaagg cggcgagctt
    16801 tttggctttg tcgcgtttga aggctttgac ggcttcaaat tcggcgcgca ggttttctaa
    16861 aaaaaggccg ttgatgactt tgtcgatgtt ggcggtttcg gtgtagtgtg cgcccgcttc
    16921 gcgccgctct tgtgcgtcca tgactgattg gaagagcgtg ccgaagattt cgggactgat
//
LOCUS       DAXHTY010000039        16690 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXHTY010000039 DAXHTY010000000
VERSION     DAXHTY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16690
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.039"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            208..1272
                     /locus_tag="ACFNMK_001063"
     CDS             208..1272
                     /locus_tag="ACFNMK_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC6364348.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1413..1736
                     /locus_tag="ACFNMK_001064"
     CDS             1413..1736
                     /locus_tag="ACFNMK_001064"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC6364349.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1808..2131
                     /locus_tag="ACFNMK_001065"
     CDS             1808..2131
                     /locus_tag="ACFNMK_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC6364350.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2178..2381
                     /gene="tatA"
                     /locus_tag="ACFNMK_001066"
     CDS             2178..2381
                     /gene="tatA"
                     /locus_tag="ACFNMK_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC6364351.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2385..3071
                     /gene="tatB"
                     /locus_tag="ACFNMK_001067"
     CDS             2385..3071
                     /gene="tatB"
                     /locus_tag="ACFNMK_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC6364352.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLSDGISDVMPSERSDTSAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3085..3855
                     /gene="tatC"
                     /locus_tag="ACFNMK_001068"
     CDS             3085..3855
                     /gene="tatC"
                     /locus_tag="ACFNMK_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC6364353.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKISVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3908..4516
                     /locus_tag="ACFNMK_001069"
     CDS             3908..4516
                     /locus_tag="ACFNMK_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC6364354.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVIQLPAGIDESVKADETPACY
                     VQRMAAEKNQTALALFLETNGAMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRSPLSALKP"
     gene            4525..4878
                     /locus_tag="ACFNMK_001070"
     CDS             4525..4878
                     /locus_tag="ACFNMK_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC6364355.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4868..6682
                     /locus_tag="ACFNMK_001071"
     CDS             4868..6682
                     /locus_tag="ACFNMK_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC6364356.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     LQNLIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6872..7549
                     /gene="misR"
                     /locus_tag="ACFNMK_001072"
     CDS             6872..7549
                     /gene="misR"
                     /locus_tag="ACFNMK_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC6364357.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7563..8969
                     /gene="misS"
                     /locus_tag="ACFNMK_001073"
     CDS             7563..8969
                     /gene="misS"
                     /locus_tag="ACFNMK_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC6364358.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9087..11513
                     /locus_tag="ACFNMK_001074"
     CDS             9087..11513
                     /locus_tag="ACFNMK_001074"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6364359.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPVEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHSLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HLKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11558..11803
                     /gene="rpsP"
                     /locus_tag="ACFNMK_001075"
     CDS             11558..11803
                     /gene="rpsP"
                     /locus_tag="ACFNMK_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC6364360.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11819..12328
                     /gene="rimM"
                     /locus_tag="ACFNMK_001076"
     CDS             11819..12328
                     /gene="rimM"
                     /locus_tag="ACFNMK_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC6364361.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTVSVTLEAGKVVNSELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12328..13077
                     /gene="trmD"
                     /locus_tag="ACFNMK_001077"
     CDS             12328..13077
                     /gene="trmD"
                     /locus_tag="ACFNMK_001077"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC6364362.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRSLIPKESRILNKILQEQREIQS"
     gene            13093..13458
                     /gene="rplS"
                     /locus_tag="ACFNMK_001078"
     CDS             13093..13458
                     /gene="rplS"
                     /locus_tag="ACFNMK_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC6364363.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13780..14505
                     /locus_tag="ACFNMK_001079"
     CDS             13780..14505
                     /locus_tag="ACFNMK_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC6364364.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14529..15404
                     /locus_tag="ACFNMK_001080"
     CDS             14529..15404
                     /locus_tag="ACFNMK_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC6364365.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALVSVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15481..16395
                     /locus_tag="ACFNMK_001081"
     CDS             15481..16395
                     /locus_tag="ACFNMK_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFC6364366.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSSDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4359 a         5312 c         3663 g         3356 t
ORIGIN      
        1 cgtctgaagc cgattcggtt ttcagacgac atcattattt gacctgacgc aaacagaaag
       61 ccttccgcgt gcttaaacac atctcttgta gtaaaatatc taaacagatt caactggaaa
      121 cggtcaggct tcttcacgcc attcccattt caaccgccgc ctgccagatt cagacggtat
      181 tcctacttga aaacaaggag cgttacaatg aaagcagcac gtttttacga taaaggcgac
      241 atccgcatcg aagacatccc cgaaccgacc gtcgcccccg gcactgtcgg catcaatgtc
      301 gcctggtgcg gcatctgcgg tactgacctg cacgaattca tggaaggccc gattttcatt
      361 ccgccttgcg gtcatccgca cccgatttcc ggcgagtccg cacccgtaac gatgggacac
      421 gagttctccg gcgtggtcta tgccgtcggc gaaggcgtgg acgacatcaa agtcggccaa
      481 cacgtcgtgg tcgaacccta catcatccgc gatgacgtac cgaccggaga aggcagcaac
      541 taccacctct ccaaagatat gaactttatc ggcttgggcg gctgcggtgg cggtctgtcc
      601 gaaaaaatcg ccgtcaaacg tcgttgggta catccgattt ccgacaaaat cccgttggat
      661 caagccgctt tgatcgaacc gctgtctgtc ggacaccacg cctatgtacg cagcggcgcg
      721 aaagcaggcg acgtcgcatt ggtcggcggc gcaggtccga tcggtttgct gttggctgcc
      781 gtgttgaaag ccaaaggcat caaagtcatc atcaccgagt tgagtaaagc acgcaaagac
      841 aaagcgcgcg aatccggcgt tgccgactac atcctcgacc cgtccgaagt cgatgttgtc
      901 gaagaggtga aaaaactgac caacggcgaa ggcgtggacg tggcattcga atgtaccagc
      961 gtgaacaaag tgttggatac tttggtcgaa gcctgcaaac ctgccgccaa tttggttatc
     1021 gtatccatct ggagccaccc tgcaaccgtc aacgtccaca gcgtcgtgat gaaagagctg
     1081 gatgtgcgcg gcacgattgc ctactgtaac gaccacgccg aaaccatcaa actggtcgaa
     1141 gaaggcaaaa tcaacctcga acccttcatc acccagcgca tcaagctgga cgaactggtt
     1201 tccgaaggct tcgagcgtct gatccacaac aacgaatccg ccgttaaaat tattgtgagt
     1261 ccaaacctgt aagcaaatta catttgcaac agcacaaatg ccgtctgaaa cgctcagacg
     1321 gcattttcca acgaagcgca acaggcgtat tgcccgagag cagccgttta gcctatcatt
     1381 ccgaacaaac agaaacaaac agaaggaaaa tcatgggaga ttccgtacta tccgccatcc
     1441 aacaaaccat cacccagcga aaatctgcca atccgtccga atcttacgtc gcacagctct
     1501 tgcataaggg cgaagacaaa atcctaaaaa aagtgattga agaggcgggc gaagtgttga
     1561 tggcatccaa agacaaaaac ccgtcccacc tggtttacga agttgccgac ttatggtttc
     1621 acaccatgat tcttctgaca caccacgacc tgaaggcgga agacgtattg gacgaacttg
     1681 cgcgccgcca aggtttgtcg ggcttggccg aaaaagccgc tcgcacagaa tcttgaattt
     1741 atattaaaat ccgcactttc ccacattcaa tccgtctgac cgctgttcag acggcatcgg
     1801 agccgttatg gacaactgta ttttctgcaa aatcgccgcc aaagacattc cggcgcaaac
     1861 cgtctatgaa gacggcgaaa tggtttgttt caaagacatc aaccccgctg ctccggttca
     1921 tctgctgctg attcccaaag tccatttcga ttcgttggca cacgccgcgc ccgaacatca
     1981 gccccttttg ggaaaaatga tgctgaaagt tcccgaaatc gccaaagcgg caggactggc
     2041 agacggcttc aaaaccctga tcaacaccgg aaaaggcggc ggacaagagg tcttccacct
     2101 gcatatacac atcatgggca cacccgtata aaccgttatt tcacaatcaa cccctaatac
     2161 ttacttaagg atacatcatg ggcagttttt ctctgacgca ctggattatc gtactgatta
     2221 tcgtcgtttt gatattcggc accaaaaaac tgcgcaacgt cggcaaagac ctcggcggtg
     2281 cggttcatga cttcaaacag gggctgaacg aaggtacaga cggcaaagaa gcccaaaaag
     2341 acgatgtaat cgaacacaaa aaagacgaag acaaagcgta atttatgttt gatttcggtt
     2401 tgggcgagct ggtttttgtc ggcattatcg ccctgattgt cctcggcccc gaacgcctgc
     2461 ccgaggccgc ccgcaccgcc ggacggctca tcggcaggct gcaacgcttt gtcggcagcg
     2521 tcaaacagga atttgacact caaatcgaac tggaagaact gaggaaggca aagcaggaat
     2581 tcgaagctgc cgctgctcag gttcgagaca gcctcaaaga aaccggtacg gatatgcaga
     2641 acagtctgca cgatatttcc gacggtctga agccttggga aaaactgccc gaacagcgca
     2701 cgcctgctga tttcggtgtc gatgaaaacg gcaatccctt tcccgatgcg gcaaacaccc
     2761 tatcagacgg catttccgac gttatgccgt ccgaacgttc cgatacttcc gccgaaaccc
     2821 ttggggacag cgggcaaacc ggcagtacag ccgaacccgc ggaaaccgac caagaccgcg
     2881 catggcggga atacctgact gcttctgccg ccgcacccgt cgtacagacc gtcgaagtca
     2941 gctatatcga taccgctgtt gaaacccctg ttccgcacac cacttcgctg cgtaaacagg
     3001 caataagccg caaacgcgat ttgcgtccta aatcccgcgc caaacctaaa ttgcgcgtcc
     3061 gtaaatcata aagagggcaa tccggtgtcc gaaacacaaa acgaacaacc cgtccaaccg
     3121 cttgtcgaac acctcatcga gctgcgccgc cgcctgatgt ggacggttgt cggtatctta
     3181 gtctgctttt tcggcctaat gccgtttgcc caacaactct atacttttat cgccgacccg
     3241 ctgatggcaa acctgcccaa agacaccagc atgattgcca cagatgtcat cgcaccgttt
     3301 ttcgttccgg tcaaagttac cctgatggcg gcatttttaa tttcgctgcc gcatacgctc
     3361 taccaaatct gggcatttgt cgcccccgca ctctatcaaa acgaaaaacg cctgattacc
     3421 ccgcttgtcc tctcaagcgt cagcctgttt ttcatcggca tggcatttgc ctactttctg
     3481 gttttccccg tcattttcaa attccttgcc agcgttaccc ctgtcggtgt caatatggcg
     3541 acagacatcg acaaatacct atcttttatc ttaggcatgt ttgttgcgtt cggcacaacg
     3601 tttgaagttc ccatcgtcgt tatcctgtta accaaaatca gcgtggtaac aaccggacag
     3661 ctcaaacgcg cccgccccta tgtgattgtc ggcgcgtttg tcattgccgc catcatcacg
     3721 ccgcccgatg tgatttcaca aaccctgctt gccattccgc tgattctctt atacgaagca
     3781 ggtatttggt tcggacgctt tttcacgcca cgttcagaac aggatggcga catacagccg
     3841 cctgcaacaa cctgacacta tgccgtccga acctccgcct cataccgcaa cagattaagg
     3901 aatacctttg aataccctct atttaggttc aaacagcccg cgccgaatgg aaatcctgac
     3961 acagttgggc taccgcgtca tccaactgcc cgccggtatc gacgaatccg ttaaagccga
     4021 tgaaacacct gcctgttacg ttcaaagaat ggcggcagaa aaaaatcaga cggctttggc
     4081 cctgtttctc gaaaccaacg gcgcaatgcc tgactttccc ttgattacag cggatacctg
     4141 cgtcgtttca gacggcatca tattgggcaa accccgctcc caagctgaag caatcgaatt
     4201 tttaaaccga ttgtccggca aacaacatac cgtcctgact gctgtctgca ttcattatcg
     4261 cggcaaaacg tcaagccgcg tccaaaccaa ccgcgtcgtt ttcaagcccc tgagttcgga
     4321 agaaatttcc gcctatgtgc aaagcggcga accgatggac aaagccggcg cctacgccgt
     4381 acaaggcata ggcggcatct ttatccaatc tatcgaaggc agcttcagcg gcattatggg
     4441 actgcccgtt tatgaaaccg tttcgatgtt gcaggatttg ggataccgct cccccttgtc
     4501 cgcccttaaa ccgtaaagac cgccgtgaac agacaaaccg cttacctcct tacctctttc
     4561 agcctgatcg cactgataat cctgtccctt tcctgggaac tgtggattgc accgttgcgc
     4621 ccgggcggct cgtggctcgc gctcaaagcc ctccccctct gtctgccgct ttcaggcatc
     4681 ctgaagaaaa aaatctacac ctaccaatac agctccatgc tggttctgat ttactttgcc
     4741 gaagccgtca tgcgcctgtt cgacgcctat cccgcagaac agatttgcgc cgcgctttcc
     4801 gcagtattca gcatcatctt cttcatatcc tgcctgtcgt tcgtcaaaca atacaaggaa
     4861 acaaacaatg cccgctgaaa cgaccgtatc cggcgcgcac cccgccgcca aactgccgat
     4921 ttacatcctg ccctgcttcc tttggatagg catcgtcccc tttaccttcg cgctcaaact
     4981 gaaaccgtcg cccgactttt accacgatgc cgccgccgca gccggcctga ttgtcctgtt
     5041 gttcctcacg gcaggaaaaa agctgtttga tgtcaaaatc ccacctatca gcttccttct
     5101 gtttgcaatg gcggcgtttt ggtatcttca ggcacgcctg atgaacctga tttaccccgg
     5161 tatgaacgac atcgtctctt ggattttcat cttgctcgcc gtcagcgcgt gggcctgccg
     5221 gagcttggtc gcacacttcg gacaagaacg catcgtgacc ctgtttgcct ggtcgctgct
     5281 tatcggctcc ctgcttcaat cctgcatcgt cgtcatccag tttgccggct gggaagacac
     5341 ccccctgctt caaaacctca tcgtttacag cgggcaaggc gtaatcggac acatcgggca
     5401 gcgcaacaac ctcggacact acctgatgtg gggcatactc gccgccgcct acctcaacgg
     5461 acaacgaaaa atcccgcccg ccttgggcgc aatctgcctg attatgcaga ccgccgtttt
     5521 aggtttggtc aattcgcgca ccatcttgac ctacatagcc gccatcgccc tcatccttcc
     5581 cttctggtat ttccgttcgg acaaatccaa caggcggacg atgctcggca tagccgcagc
     5641 cgtattcctt accgcgctgt tccaattttc catgaacacc attctggaaa cctttaccgg
     5701 catccgctac gaaactgccg tcgaacgcgt tgccaacggc ggtttcacag acttgccgcg
     5761 ccaaatcgaa tggaataaag cccttgccgc cttccagtcc gccccgatat tcgggcacgg
     5821 ctggaacagt tttgcccaac aaaccttcct catcaatgcc gaacagcaca acatatacga
     5881 caacctcctc agcaacttgt tcacccattc ccacaacatc gtcctccaac tccttgcaga
     5941 gatgggaatc agcggcacgc ttctggttgc cgcaaccctg ctgacgggca ttgccgggct
     6001 gcttaaacgc cccctgaccc ccgcatcgct tttcctaatc tgcacgcttg ccgtcagtat
     6061 gtgccacagt atgctcgaat atcctttgtg gtatgtctat ttcctcatcc ctttcggact
     6121 gatgctcttc ctgtcccccg cagaggcttc agacggcatc gccttcaaaa aagccgccaa
     6181 tctcggcata ctgaccgcct ccgccgccat attcgcagga ttgctgcact tggactggac
     6241 atacacccgg ctggttaacg ccttttcccc cgccactgac gacagtgcca aaaccctcaa
     6301 ccggaaaatc aacgagttgc gctatatttc cgcaaacagt ccgatgctgt ccttttatgc
     6361 cgacttctcc ctcgtaaact tcgccctgcc ggaatacccc gaaacccaga cttgggcgga
     6421 agaagcaacc ctcaaatcac taaaataccg cccccactcc gccacctacc gcatcgccct
     6481 ctacctgatg cggcaaggca aagttgcaga agcaaaacaa tggatgcggg cgacacagtc
     6541 ctattacccc tacctgatgc cccgatacgc cgacgaaatc cgcaaactgc ccgtatgggc
     6601 accgctgcta cccgaactgc tcaaagactg caaagccttc gccgccgcgc ccggtcatcc
     6661 ggaagcaaaa ccctgcaaat gacccgcgcc ggcggatgcg gataccgccc gaaatgtaaa
     6721 gctccatgca agacattgca aaaaacagca aaccggtagg gaaatacgct atcaaaaaac
     6781 attgcagccg tgttaagata aaccgtcaaa caatcttttc acgccccgcc cgaaacaggg
     6841 tcggggcata cccttacgaa aaggaaacac catgagccgc gtattactcg tagatgacga
     6901 tgccctgcta accgaactgc tgaccgaata cctgagcgcc gaaggtctga acgtccgcag
     6961 cgttcccgac ggggaagcag gcgtacagga aatcctgagc gggcagtacg atgtagtcgt
     7021 attggattcc atgatgccca aaatgaacgg cttggatgtc ttgaaaaacg tacgcgcccg
     7081 aagcaccgtc cccatcatca tgctgaccgc caaaggcgac gacatcgacc gaatcatcgg
     7141 cttggaaatg ggcgcggacg actatgtccc caaaccctgc acaccacgcg aactcttggc
     7201 acgcatcaat gccatcctgc gccgcgcaca acacagcggc gaacagaaca acgcacccaa
     7261 cagcatctcc gtcagcgatg tcgtcctgta tcccgccaaa cgccaggcat ccgtcaaaga
     7321 catgccgctc gaactgacca gcaccgaatt caacctgctc gaagtcctga tgcgccatgc
     7381 cggacaggta gtcagcaaag aaaccctgtc cgtcgaagca ctcgaccgca agctggcaaa
     7441 attcgaccgc agtatcgacg tacacatctc cagcatccgc cacaagttgg gcgatgcctc
     7501 tctgattcaa accgtacgcg gcttgggcta cctgtttgtc aaaaactgaa ataaacagat
     7561 aaatgaaact gttccaacgc atcttcgcca cattttgcgc ggttatcgtc tgtgcaatct
     7621 ttgtggcgag tttttctttc tggctggtgc agaacaccct tgccgaaaac cagttcaacc
     7681 aacgccgcac catcgaaacc actttgatgg gcagcatcat ttccgcattc cgggcacgcg
     7741 gggacgcggg cgcgcgcgaa atcctgacgg aatggaaaga cagccccgtc tcatcgggcg
     7801 tgtacgttat acagggcgac gagaaaaaag atatcctgaa ccggtatatc gacagctaca
     7861 ccatcgaacg cgcccggctt ttcgccgccg gacacccgca ttccaacctc gtccatatcg
     7921 aatacgaccg cttcggcgaa gaatacctgt tcttcaccaa agactgggac aaactccaag
     7981 cccgccgcct gcccagcccc ctgttgatcc ccggcctgcc gctcgccccg atttggcacg
     8041 aactcatcat attgtccttc atcatcgtcg tcggactgct gatggcgtac atcctcgccg
     8101 gcaacattgc caaacccatc agaatcttag gcaacggcat ggacagggtg gcaaacggag
     8161 aacttgaaac ccgtatctcc caacaggtcg acgaccgcga cgacgaattg tcccatcttg
     8221 ccatccaatt cgacaaaatg gtggaaaaac tcgaaaaact cgttgccaaa gaacgccacc
     8281 tgctccatca cgtctcccat gaaatgcgtt ctccccttgc gcgcatgcag gcaattgtcg
     8341 gactgattca ggcgcagccc caaaaacagg agcaatatct caaacggctg gaaggcgaac
     8401 tgacccgcat ggatacgctg gccggggaac tgttaaccct gtcccgtctc gaaacttcca
     8461 atatggcttt ggaaaaagaa agcctgaaac tcctgccctt cctgggcaac ctggtagaag
     8521 acaatcaaag cattgcccag aaaaacggac aaacggttac cctgtctgcc gacggaaaaa
     8581 tccccgaaaa cacaaccatc cttgccaacg aaagctacct gtaccgcgcc ttcgacaacg
     8641 tcatccgcaa cgccgtcaac tacagtcccg aaggcagcac catcctgatc aacatcggac
     8701 aagaccacaa acactggata atcgacgtta ccgacaacgg ccccggcgtg gacgaaatgc
     8761 agctcccgca catcttcacc gctttctacc gtgcagactc cagtgccaac aaacccggaa
     8821 caggactggg gcttgcattg acccaacata ttattgaaca gcactgcggc aaaatcatcg
     8881 ccgaaaacat caaaccgaac gggctgcgga tgcgcttcat cctacccaag aaaaaaaccg
     8941 gttccaaaac agaaaaaagt gcgaactgac cataatgccg tctgaaccgg cttcagacgg
     9001 cattgcacaa acagttaccc caaaacaaac acacagcggg tgcatctcca aaaatcacgc
     9061 acataccgtc cgcaaaggaa tatatcatgt cggcacaaac cgatcccggc tacttcttca
     9121 tgcccaacca cattatcctg ataggcgcga gcgaacgtcc gtacagcctg ggcgaacgcg
     9181 tactcagcaa cctgctgagt acgccctttc aaggaaaaat cacccccgta aacccgcgcc
     9241 accacaccat agccgggctt cccgcctaca ccagcctcaa caaaatcccc ggcaatgcag
     9301 acctgattat tgccgttacc ctacccgaca gttacgacac cctcttcaaa acctgccgca
     9361 aaaagcagct ccgacacatc atcctcatac aggactggga cagcctgtct gccgcagaac
     9421 tgcacaccgc cgaaactgcc atccgcaaac accacggcaa cggactcaac atcaccgcct
     9481 gcaccaccgc aggcatccaa ctgccctcac tcggacttaa catcagtacc caagacggat
     9541 acgccgcagg ccataccgcc atactgaccg gcaatgccgc cgtcagccgc caaatcgaca
     9601 acatcctgaa caaactccgt caaggcacat cccgccacat cagcctgcat cccggcatca
     9661 gccccatcac atccgccgat tggctcaacc gcttcggaca cagcctgcac accaaaaccg
     9721 ccgtcctaca ccacaaccct gaagaggatc agcgcaaact gttcagcgca atccgccaat
     9781 ttacccgcca tacgccgctg attctccaca tcacctgccg cacgacagaa accgaccgtg
     9841 ccgtactgca ctgcctcgcc cgacactgca acttcctcat cagtttcaac gccgacgacc
     9901 tcgaagccgc actgcgcgcc caactgtccg accttccacc cctgtcccga ctcgacatcc
     9961 tgtccgacac gcctgtcgaa tggctgaacg cgcacgcgcc aaaaaccctc gccctccact
    10021 ttcccaacct tccccaccac atccgcaacg gacacctgac cggcacaccc acaccctcaa
    10081 tctgccacga catcgcctca cgtcagcttg cccgccccga cacccaagcc gtcctgacca
    10141 tcctcagccc ctccggacac gaggattaca aaaaaacagc acgcgccctt atccgcctgt
    10201 ccgaacagac cgccaaaccc ctgctcgtca gcagcccctt ttcagacggc ataacacatt
    10261 tcgacacccc cactcaggca atccgcaccc tttcctaccg caacaccgcc gccgccctga
    10321 aacaggcaca gctcgacatt gcaccgccgc aaccgtgccg tctgaaaaca ccgcaacccc
    10381 aaaacatcaa aaaagccctc gaagccgccg accccgccct aatcgccgaa gccctgcacc
    10441 tcccccccta ccggcacacc acccataacg ccgtccaatt ccaattcggc agccactccc
    10501 tctatggcga catcctgacc gcacgttgca acggacaaac cactgccgta ctcccgccgt
    10561 ttaccacgct cgacagccgc caccttgccc gctttgccga actcgacggc acacaaaccc
    10621 tcgaccagtt cctgcacaca ctgaccatca ttcccgaata ccgccaacac attctcggca
    10681 tcaccctcaa cctaaacggc ggacaataca gcagcgattt catcttaaag acacccgaaa
    10741 cacacgacac gcccaaacgc aagaacacag gcaaagccgc ccaaaccctc gaacatgccg
    10801 ccgcaaaaat gcagagtgcc gccgcatacc tgaaacacaa aaacccgaca gccgccgaat
    10861 ttctccgcca cacaagcgaa gccgccgcag aactgctcgg cagcaaaacc gaaaccggag
    10921 cagccgtacc caacgtactt gccccctatc ccgcagcaca cctcaaaaca ctgtccctaa
    10981 gaaacaacac gaccgttacc attaccccca ttttgcccga agacgcagaa gccaaacagc
    11041 agttcgtccg cagcctcggt cccgaagcac ggtacacacg cttcatgacc cacaccaacg
    11101 aactgcccgc agccacgttg gcacgcctgt gcaaccccga ttaccactgt gaagccgcat
    11161 ggacggcaaa ggatgccgac agcaacatcg tcgccgtcgt ccgccacagc cgcctgaacc
    11221 gcaacgaatg cgaatttggc atcacactgg cggaacatat gcgcggcagc ggactggcac
    11281 agaaaatgat ggaactcatc atccaaaccg ccgcacagca aggctaccgg actatgagtg
    11341 ccgacattct caaaaccaat acccccatga tcaaacttgc tgaaaaatca ggatttaccc
    11401 tcaaggaatc ggacaccgaa aaaaacctgt accgcgcata tctgaacctt gcggcagaca
    11461 aaacaacaga aaaaacaaat aaaaacttgc gcaccgacca caaaataacc taaaatcggc
    11521 agtttccata tatccgattt tctcaaaagg actcaaaatg gtagttatcc gtttggcacg
    11581 cggcggctcg aaacaccgcc ccttctacaa cgtcatcgtt actgactcac gcagccgccg
    11641 cgacggccgc ttcatcgaac gcgtaggctt ctacaacccc gtagccaacg aaaaacaaga
    11701 gcgcgtccgc ctcaatgcag accgcctgaa ccactggatt gcacaaggcg cgcaagtcag
    11761 cgactccgtt gcaaaactga ttaaagaaca aaaagccgcc taattcacat ttgccgccat
    11821 gacagacact caaaaccggg tagccatggg ctacatcaaa ggcgtattcg gcataaaagg
    11881 ctggttgaaa attgccgcca acaccgaata ttccgacagc cttttggact accccgagtg
    11941 gcatttggtc aaggacggca aaaccgtcag cgttaccctt gaagccggaa aagtcgtcaa
    12001 cagcgaactc caagtcaaat tcgaaggcat aaacgaccgc gacttggcat tctcattgcg
    12061 cggttacacc atcgaaatac cccgtgaagc attcgccccg acagaagaag acgaatacta
    12121 ctggacagac ttggtcggca tgaccgtcgt caacaaagac cataccgttt taggcaaggt
    12181 aagcaacctg atggaaaccg gcgcaaacga cgtattgatg attgacggag aacacgggca
    12241 gattctgatt ccgttcgttt cccaatatat cgaaaccgtc gataccggca gcaagattat
    12301 tactgccgac tggggtttgg actactgatg cttatccagg cagttaccat tttccccgaa
    12361 atgttcgaca gcattacccg ctacggcgta acgggacgcg cgaacagaca gggaatctgg
    12421 cagtttgaag cagtcaatcc ccgaaagttt gccgacaaca gattgggcta catcgacgac
    12481 cgcccgttcg gcggcggccc gggaatgatt atgatggctc cgccgcttca tgcagcaata
    12541 gaacacgcca aagcacaatc ctcccaagct gcaaaagtca tctacctcag cccccaaggg
    12601 aaaccgctga cacaccaaaa agcggtagaa ctggcagaac ttccgcatct gattctgctg
    12661 tgcggacggt atgagggcat agacgaaagg cttctgcaaa gcagcgtcga tgaagaaatc
    12721 agcatcggag acttcgttgt ttccggcgga gagcttcccg ccatgatgct gatggatgcg
    12781 gtattgaggc tcgtacccgg cgtattgggc gatatgcagt ctgccgaaca agattcgttc
    12841 tcaagcggca ttttggactg cccccactac accaaaccct tagaatttca aggcatggct
    12901 gttccggaag tattacgttc cggcaatcat ggcttgatag cggaatggcg gttggaacaa
    12961 tcgctgcgcc gcaccttgga gcgcagaccc gatcttttgg aaaagcgcag tttaatccca
    13021 aaggaatccc gcatcttgaa taaaatccta caagagcaac gggaaatcca atcataattt
    13081 aggaaaaaaa caatgaacct gattcaacag ctcgagcaag aagaaattgc ccgcctgaac
    13141 aaagaaatcc ccgaattcgc accgggcgac accgtagtcg tatccgtacg cgtcgtggaa
    13201 ggtacccgca gccgtctgca agcctacgaa ggcgttgtca ttgcacgccg caaccgtggt
    13261 ctgaacagca acttcatcgt ccgtaaaatt tccagcggcg aaggtgttga acgtactttc
    13321 caactgtact ctccgaccgt cgaaaaaatc gaagtcaaac gccgtggcga cgtacgccgt
    13381 gccaaactgt actacctgcg cggtctgacc ggcaaagctg cacgcatcaa agaaaaactg
    13441 cctgcacgca aaggttgatt caaacccgtt ttccccaatg ccgtctgaac cttctgacgg
    13501 catttcttat tgccgcaaca tccgcaacgg aacagcacca agaagcacac cccttccggc
    13561 acatcccgaa tccccgtccg aaccgaacaa aatttacaga ccaaacgtta tacagtatca
    13621 tatcagccat ataataataa ccgctttacc gctttccctt tcaaaaccgc aaatcatcat
    13681 gacaccttcc cttttactat caggattgac cttccgcctc gtccttgccc tgattgccgt
    13741 atccctttta tggggcgttt acttttgggc agtatccgca tgagcatcat tgtcgaaaac
    13801 ctgaccgtca gctaccgccg ccgccccgcc gtgcaccatg tggacattac ttttgaagaa
    13861 catagtatgt gggcggtttt cggtcccaac ggcgcaggga aatctacctt tcttaaatcc
    13921 ttgatgggat tgcagcccat cgacactggc agcatccggc tggacggatt gacccgtcag
    13981 aacattgcct accttcccca gcagtccgac atcgaccgct cgcagcccat gaccgttttc
    14041 gacttggcgg caatggggct gtggtatgaa atcggctttt tcaaagggat aaataccgct
    14101 caaaaacaac gcgttcacga agccttggag cgtgtaggaa tgcggcagtt tgcccgccgc
    14161 caaatcgccc acctctcaaa cggacaattc caacgcgtcc tttttgcccg aatgctggtt
    14221 caaaacgcca aattcctact tctagacgaa cctttcaacg ctgttgatgc acggacaacc
    14281 tacgaacttc tcgacgtatt gcagaaatgc cattgcggcg gacacgctat cattgccgtt
    14341 ttgcacgatt acgaacaagt ccgtgcctac tttcccaata ccctgctgct cgcccgagaa
    14401 aaaattgcgg caggcgcaac cgagaccatt ctgacagaac cctacctcgc ccaagccaac
    14461 gccaaaatgc agcaacagga aagccccgag tggtgcgcgt cataaatgcc gtctgaaacc
    14521 gaaaaaccat gaacctctac gacctgctcc ttgccccctt tgcagaattt gactttatgc
    14581 gctacgccct cgtatccgtc ttctgcctgt ccctcagtgc cgcacccgtc ggcgtattcc
    14641 tcgtcatgcg ccgtatgagc ctgataggcg acgcattgag ccacgctgtc ctgcccggtg
    14701 ccgccgtcgg ctacatgttt gccggcttga gcctgcccgc catgggtttg ggcggcgtag
    14761 ccgcaggcat gctgatggca ctgcttgccg gacttgttag ccgcttcacc accctgaaag
    14821 aagatgccaa ctttgccgcc ttttatctca gcagcctcgc cataggcgta gtcctcgtca
    14881 gcaaaaacgg cagcagtgtc gatctgctgc atctgctttt cggctctgta cttgccgtcg
    14941 atattcctgc cctgcagctc atcgccgccg tatccaccct cacactgctt acccttgccg
    15001 tcatctaccg cccgctcgta ctcgaaagca tcgaccccct gtttctcaaa tctgtcggcg
    15061 gcaaaggcgg gctttggcac gtcctctttc tcgtcctggt cgtcatgaac ctcgtatccg
    15121 gctttcaagc cctcggcaca ctcatgtccg tcggactcat gatgctgcca gccattaccg
    15181 cccgcctgtg ggcgaagcac atgggcgcac tcatcctcct atccgttctg acagccctgc
    15241 tgtgcggctt gagcgggctg ctcatttcct accacatcga aatcccttcc ggccccgcca
    15301 tcatcctctg ttgcagcgtc ctttatctct tttccgtcat actcggcaaa gaaggcggca
    15361 ttctgaccaa atggctcaaa aaccaccgcc accacaccac ctgaccccgc aagccgcttg
    15421 aacaaacagg cagcttgttt taccataaca gatgttacgt tatatcacag gagaacagtt
    15481 atgaaacacc tcaaactcac ccttattgcc gcattgctaa ccaccgccgc aactgccgcc
    15541 cccctgcccg tcgtaaccag cttcagcatt ttaggcgacg ttgccaagca aatcggcgga
    15601 gagcgcgtat ccatacaaag tttggtcgga gccaaccaag atactcatgc ctatcacatg
    15661 accagtagcg acattaaaaa aatccgcagt gcaaaactcg tcctgctcaa cggcttagga
    15721 cttgaagctg ccgatgtaca acgtgccgtc aaacagagca aagtatccta tgccgaagcg
    15781 accaaaggca tccaacccct caaagccgaa gaagaaggcg gacaccatca tgaccacgac
    15841 catgaccacg aaggacacca ccatgaccac ggcgaatttg acccgcacgt ctggaacgac
    15901 cccgtcctta tgtccgccta tgcccaaaac gtcgccgaag ccctgataaa ggccgacccc
    15961 gaaggcaaag tttattatca acaacgcttg ggcaactacc aaatgcagct taaaaaactg
    16021 cacagcgacg cacaagccgc atttaacgcc gtccctgccg ccaaacgcaa agtcctgacc
    16081 gggcacgatg ccttttccta catgggcaaa cgttaccata tcgaattcat cgccccgcaa
    16141 ggcgtgagca gcgaagccga gccctcagcc aaacaagtcg ccgccatcat ccgacaaatc
    16201 aaacgcgaag gcatcaaagc cgtctttacc gaaaacatca aggacacccg tatggttgac
    16261 cgtatcgcca aagaaaccgg tgtcaacgtc agcggcaaac tgtattccga cgcactcggc
    16321 aacgcgcccg cagacaccta catcggaatg taccgccaca acatcaaagc cttgaccaac
    16381 gcgatgaagc aataaaaccg tttgattggt ttgaatttga aaaggcaaaa ggccgtctga
    16441 aaattatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacagata
    16501 gtacggtaag gcgaggcaac gctgtactgg tttttgtgta tccactatat tgctagaagt
    16561 gcaccgatta aggttaatta aaatacgacc cttatcccaa tattaagaaa aacagcaatc
    16621 aaaataaact tgattgctgt ttttcagttt aagccatgaa tgtaattagg ctccttatgc
    16681 agatggcgtt
//
LOCUS       DAXHTY010000040        16255 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXHTY010000040 DAXHTY010000000
VERSION     DAXHTY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.040"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(86..2191)
                     /gene="fusA"
                     /locus_tag="ACFNMK_001082"
     CDS             complement(86..2191)
                     /gene="fusA"
                     /locus_tag="ACFNMK_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC6364367.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="ACFNMK_001083"
     CDS             complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="ACFNMK_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC6364368.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="ACFNMK_001084"
     CDS             complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="ACFNMK_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC6364369.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            complement(3348..3497)
                     /locus_tag="ACFNMK_001085"
     CDS             complement(3348..3497)
                     /locus_tag="ACFNMK_001085"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6364370.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            complement(3494..3751)
                     /locus_tag="ACFNMK_001086"
     CDS             complement(3494..3751)
                     /locus_tag="ACFNMK_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364371.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGNRMGMPINIG"
     gene            complement(4043..8218)
                     /gene="rpoC"
                     /locus_tag="ACFNMK_001087"
     CDS             complement(4043..8218)
                     /gene="rpoC"
                     /locus_tag="ACFNMK_001087"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC6364372.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            complement(8375..12553)
                     /gene="rpoB"
                     /locus_tag="ACFNMK_001088"
     CDS             complement(8375..12553)
                     /gene="rpoB"
                     /locus_tag="ACFNMK_001088"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC6364373.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVADRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLADEDLAKQLELIKLSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKASELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(12744..13115)
                     /gene="rplL"
                     /locus_tag="ACFNMK_001089"
     CDS             complement(12744..13115)
                     /gene="rplL"
                     /locus_tag="ACFNMK_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC6364374.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            complement(13173..13673)
                     /gene="rplJ"
                     /locus_tag="ACFNMK_001090"
     CDS             complement(13173..13673)
                     /gene="rplJ"
                     /locus_tag="ACFNMK_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC6364375.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            complement(13903..14598)
                     /gene="rplA"
                     /locus_tag="ACFNMK_001091"
     CDS             complement(13903..14598)
                     /gene="rplA"
                     /locus_tag="ACFNMK_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC6364376.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            complement(14598..15032)
                     /gene="rplK"
                     /locus_tag="ACFNMK_001092"
     CDS             complement(14598..15032)
                     /gene="rplK"
                     /locus_tag="ACFNMK_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC6364377.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFLMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            complement(15133..15669)
                     /gene="nusG"
                     /locus_tag="ACFNMK_001093"
     CDS             complement(15133..15669)
                     /gene="nusG"
                     /locus_tag="ACFNMK_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC6364378.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            complement(15671..15949)
                     /gene="secE"
                     /locus_tag="ACFNMK_001094"
     CDS             complement(15671..15949)
                     /gene="secE"
                     /locus_tag="ACFNMK_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC6364379.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            complement(16066..16141)
                     /locus_tag="ACFNMK_001095"
     tRNA            complement(16066..16141)
                     /locus_tag="ACFNMK_001095"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16106..16108),aa:Trp,seq:cca)
     gene            complement(16148..>16255)
                     /locus_tag="ACFNMK_001096"
     CDS             complement(16148..>16255)
                     /locus_tag="ACFNMK_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6364380.1"
                     /translation="TVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
BASE COUNT         4105 a         4207 c         3627 g         4316 t
ORIGIN      
        1 gctaattcct ttacattaaa gatcgattaa agaacaatgc cgtctgaaaa atcatttcag
       61 cctttcggac ggcctatttg cctgattagc ctttacgggc ttcagttaca gcagcagcta
      121 tgtgggcagg agcttcagaa tatttcttga actccataga gtaagtagcg cggccttggg
      181 ttgcagaacg caggtcggtc gaataaccga acatttctgc cagaggtact tcggcacgga
      241 cttttttacc gccgataccg tcatcatcca tacccaatac aacaccgcga cggcggttca
      301 agtcgcccat tacgtcgccc atgtattctt ccggggtttc cacttcaact gccataatcg
      361 gctcaagcag ggcgggagat gcttgacgca taccttcttt aaacgcttga gaagcagcca
      421 attcaaatgc caattgggaa gagtcgacat catggtaaga accgaatacc agacggatac
      481 gtacgtcaac tacaggatag ccggcaacga taccgttagg caacgtatcg cggatacctt
      541 tatcgacaga cggaatgaat tcgcgaggaa tcacaccacc tttaatttca tcgataaact
      601 cgtaaccttc accacccggc tccataggtt ccatttcaat cacaacgtga ccgtattgac
      661 ctttaccacc ggattgtttt gcatgtttgt attcagcttt aacggctttg cggatagttt
      721 cacggtaagc cacttgaggt gcaccgatat ttgcttccac accgaattcg cgtttcatac
      781 ggtcaacaat aatttccaag tgcagctcac ccataccgga aataatggtt tgaccggatt
      841 cttcgtctgt acggacacgg aaagaagggt cttctttagc caagcggttc agggcgatac
      901 ccattttctc ttggtcggct ttggttttcg gctcaacggc aatatggatt accggctcgg
      961 ggaattccat acgttccaag ataatcggcg cgctttccgc acacagggtt tcaccggtag
     1021 taacgtcttt cagaccaata gcggctgcga tgtcgccggc gcgtacttct tcgatttcag
     1081 tacggtctgc ggcagtcatt tgtaccaaac gaccgatacg ttcgcgagtg ccttttacgg
     1141 agttcaatac ggtatcgccg gattttacta cgcctgagta aacgcggata aaggtcagct
     1201 gaccgacgta tttgtcgttc aacattttga acgccaatgc agagaatttc tcttcatcgc
     1261 tggcttgacg gctgtcggct tcctcggtat tcgggttgac accttgaacc ggaggaatat
     1321 cggtaggagc tggcagcaat tctacaactg cgtccaacat acgttgaaca cctttgtttt
     1381 taaatgcaga accacacagc ataggctgaa tttcgcctgc caaagtacgt tgacgcaacg
     1441 cgcctacgat ttcttcttcg gtcagctcgt cgccgcctaa gtatttgtcc atcagttctt
     1501 cgctggcttc ggctgcggct tcaatcatat tttgacgcca ttcttcggca gtttcgacca
     1561 attcggcagg aatatcgcca taggtaaagg ttgtaccttt atcggcttca ttccaaatga
     1621 tggatttcat tttcaacaaa tcaaccacac cgctgaagtt gtcttccgca ccaaccggaa
     1681 tgacgatagg tacagggttt gcgcgcaaac gggttttcat ttgctcgaca acgcggaaga
     1741 agttggcacc ctgacggtcc attttattga caaacgccaa gcgcggcact tggtatttgt
     1801 tggcttgccg ccatacggtt tcagattggg gttgaacacc gcccaccgcg cagtaaacca
     1861 ttaccgcgcc gtccaataca cgcatagaac gctctacctc tacggtaaag tcaacgtgtc
     1921 ccggggtgtc gatgatgttg aagcggtgct cggggaattg tttcgccata ccggaccagt
     1981 aggaagtaac ggcagcagag gtaatggtaa taccacgctc ttgctcttgt tccatgtagt
     2041 cggtagtagc cgcaccgtca tgcacttcgc ccagcttgtg ggtcaaaccg gtatagaaca
     2101 aaatacgttc tgtcgtcgtg gttttacccg catcgatatg ggcggaaata ccgatgttac
     2161 ggtacaggct gatcggggtc ttacgagcca ttttattagc ctttcaaaat tagaaacgga
     2221 agtgagagaa tgctttgttg gcttcagcca tacggtgtac ttcttcacgt tttttcaacg
     2281 caccgccacg gccttcggac gcatcaatca attcgcctgc caaacgcagg tccatggatt
     2341 tctcaccacg tttgcgggcc gcatcgcgaa cccagcgcat tgccaaagcc aaacggcgtg
     2401 aaggacgaac ttcaacagga acttggtagt ttgcaccacc tacacggcgg cttttcactt
     2461 ccacgatagg tttggcgttt gcaatggctt cgttaaatac ttcgattgct actttgccgg
     2521 tttttttctc aatctgttcc aacgcaccgt aaacgatacg ctcggcaacg gattttttac
     2581 cgtcaatcat caatacgttc atgaatttgg tcaactcgac gctgccgaat ttaggatctg
     2641 gcagtacgtc gcgcttgggg acttctctac gtcttggcat tttaatttcc tttaatctat
     2701 tcagtcgggt ctattcccat gaatacccaa ttgagtattc acttactcgg ccgttgttct
     2761 gcttaggcgg ccgacgtgcc tatttaagtc ccagtaatta tttaggacgc ttagcaccat
     2821 atttggaacg ggcttgttta cggtctttaa cacctgcagt atccaaagaa ccgcgtacag
     2881 tgtggtaacg cacacctggc aagtctttta cacgaccgcc gcgaatcaat acgacactgt
     2941 gctcttgcag gttgtggcct tcgccgccga tgtatgaaat gacttcaaaa ccgttggtca
     3001 ggcggacttt acatacttta cgcaatgcag agttaggttt tttaggggta gtcgtgtata
     3061 cacgggtgca cacgccgcgt ttttgcgggc aggcttccag tgcgggcact ttgtttacgt
     3121 acacgggctt ttgacggcct ttgcgtacca attggttgat agttggcata ttttctcgtc
     3181 ctgttgagtt aaatacttgc cgacaccatg tcggcaagag tggaattata tttttattgc
     3241 aagtatacag tcaagcataa cgacaagaag aaagatgatt ttcttgtttg ccgataaata
     3301 tttaccgaca cccctcacaa tcagtcggac gactgaagaa aatactgtca atacgttttt
     3361 tctatactga caaaccgttc cttgccgctg cgcgaataaa aggcgggcat attgctgttg
     3421 atcttgttgt aacggaaatt tatttggggt gtaaatcctt tccacgacag ttttttatgc
     3481 cataaagccg cgctcatccg atgttaatcg gcattcccat ccggttaccc cgaacatcgc
     3541 cgcctgccgc gcttttagga acttcagacg gcattgcatc gcgaaccggc ggtacatcct
     3601 cggcaaacgg caggagggcg aggctttccc cttcagacgg catcgcaatc cgattaccga
     3661 cgttaatcag ttcgacaggt acgccgatat cctcggcaaa cgtcgccgaa gtcagcaggc
     3721 agcctgcaaa aaagagattt ctgggtttca tatttatttt ttaaaggaaa tgccgtctga
     3781 agtttcagac gacatttgtt taaatggaaa acattaagac gctacttttt ttgaccgccg
     3841 aaaccaacat ccctggcaaa gttattttgg tctccggacg taccgacgac acccgccacc
     3901 tcgttggtct ccggcccgaa aatacctaag gaataatcca cacgcctcaa aatgacgaaa
     3961 acgcttgttt ttgccttaat cggacaaaga aaaaccgcaa gcttaaagct tgcggttttg
     4021 tattgaataa acgcgccgaa gattattcat ccgttacttg ggtctcagca gtctcctgct
     4081 ctaccccttg ccattgctga tgacggctgc ggtgataagt caaaccggta ccggcaggaa
     4141 tcaggcggcc gacgatgacg ttctctttta aaccgcgcag ttcgtcttgt ttgcccataa
     4201 tcgcggcttc ggtcagaaca cgggtcgttt cttggaacga tgcagcagaa atgaagctgt
     4261 cggtagacag ggaggctttg gtaataccca gcaatacgtt ttcgtaacgt gccggttctt
     4321 tgccttcttc caaagctttt tcattggcag ccatcacatc gccgcgttcg acctgctcgc
     4381 cggtaatgaa gccggtttcg ccggcatccg caatgttcac acggcgcaac atttgacgga
     4441 tgatgacttc gatgtgtttg tcggaaatct tcacaccttg cagacggtaa acctcttgca
     4501 cctcttggac aatgtagcgt gccagtgctt cgataccttg cagacgcaga atatcgtgcg
     4561 gatcgaccgc gccgtccaca atggtttcgc cgcggtttac cacttgaccg tcgtgtacca
     4621 aaatctgttt ctctttggaa atcaaagtct cgtatgctac gccgtccacg tcggtaataa
     4681 tcagacgttg cttgcctttg gtctctttgc cgaaggaaac ggtaccggta atttccgcca
     4741 acataccggc atctttcggc acgcgtgctt caaacaattc ggcaacgcgc ggcagaccac
     4801 cggtaatgtc gcgggttttg gaagaggctt gcgggatacg cgccaatacg tcgcctttac
     4861 cgatttcctg accttcgcgt acggtaatca ccgcaccaac ggggaatgcc atggataccg
     4921 gagtagaagt accgggaata cagatttcca cgccgttttc gtccaagagt ttcacagtcg
     4981 gacgcagcag tttggaagca ctagaggaac gacgtttacc gtcgatcact accaaagtgg
     5041 acaaaccggt tacatcatcg gtttgtttgg caacggtaac gccctcttcc acgttttcga
     5101 atttcaccat acctgcgtgt tcggtaatca tcggacgggt atgcggatcc caggttgcca
     5161 aggtttgacc ggctttaatg gccataccgt cttgtaccag caggatggca ccgtaaggta
     5221 ctttgtggcg ttcgcgttca cggccgatgt catcgtgaat cacgacttca caagaacggc
     5281 cgatgacaac caactcgcct ttattgttgg caacataacg catttggctg ctgaatcgtg
     5341 ccgtaccgtt ggatttggct tccacttggc tggctgctgc cgcacgggat gccgcaccac
     5401 cgatgtggaa cgtacgcata gtcagctggg tacccggttc gccgattgat tgggcggcaa
     5461 tcacgccgac tgcctctccg gcattgacca gtttgccgcg cgccaagtcg cggccgtaac
     5521 agtgcgcgca caaaccgtgg cgggttttac aagtaatcgg cgtacggact ttgacttcat
     5581 cgacaccgga ttggtcaatc atatccacca gtttttcagt caacaacgtg cctgcttcaa
     5641 ccaaggtctc gccgcttgac ggatcgacaa cgtcagacgc agtaacacgg cccaaaatac
     5701 gatcgcgcaa tgcttcaatc acatcaccgc cttgtaccac tgccttcatg acaaagccgt
     5761 ctgaagtacc gcaatcgtct tcaacaacga ccaaatcttg agttacgtct accagacgac
     5821 gggtcaggta accggagttc gcggttttca atgcggtatc cgccaaaccc ttacgcgcac
     5881 cgtgggtcgc aataaagtat tgcaatacgg tcaggccttc gcggaagttt gaggtaatcg
     5941 gcgtttcaat aatcgagccg tcaggtttag ccatcaaccc gcgcataccg gacaactgtt
     6001 taatctgagc cgcagaacca cgggcaccgg agtccgccat catataaatg gagttgaatg
     6061 actcttgatc gacttcgttg ccggcacggt cgataacttt ttgtttggac aagttatcca
     6121 tcatcgcttt agcgatttta tcgccggcac gaccccaaat atccactact ttgttgtaac
     6181 gttcgccatt ggtaaccaaa ccttgacggt attggtcttc gatttcttta acctcggcat
     6241 tggcttcagc cagcaaggcc gctttttctt tcgggatttc catatcgtca acggcaatgg
     6301 aaataccgcc ttttgccgca aatccgaaac cggtgtacat caggtggtca gcaaagataa
     6361 ccgtatcgcg caagccgcac aggcggaacg atgcgttaat cagtttagaa atttcttttt
     6421 tcttcaatgc cttgttgaca tattcaaacg gcaggccttt cggcaggatt tcgctcaaca
     6481 atgcacggcc gacggttgtt tcgtaacggt taacgacagg ttcaaactca cctgcttcgt
     6541 ttttcaccca ttcgcgcaga cgtacggtaa ttttcgtacc cagctcgacc tgtttggtat
     6601 ggtatgcacg atgcacttct ttcacatcgg caaacaggct gccttcgcct ttggcattga
     6661 tacggtcgcg ggtcatgtag tacaaaccca atacgatgtc ttgggaaggt acgataatcg
     6721 gttcgccgtt ggctggagac aatacgttgt ttgaagccag catcagggtg cgcgcttcca
     6781 tttgtgcttc caagctcaat ggaacgtgta cagccatttg gtcaccgtca aagtcggcgt
     6841 taaatgcggc acacaccaat gggtgtaact gaatcgcttt accttcaatc aggataggtt
     6901 cgaacgcttg aatacccaaa cggtgcaggg ttggcgcacg gttcagcata atcggatgtt
     6961 cgcggatcac ttcttccaag atatcccata cttcaggtac ttcttgttct accagttttt
     7021 tcgcagcttt aacggtagaa gccaaacctt gtttttccaa tttgtggaaa atgaatggtt
     7081 tgaacaactc caaagccatt tttttcggca gaccgcattg gtgcagacgc aggtatgggc
     7141 ctacggtaat cacggaacgg ccggagtagt ccacacgttt acccagcagg ttttgacgga
     7201 agcgtccgcc tttaccttta atcatgtcag ccaatgattt cagcgggcgt ttgttggcgc
     7261 cggtcatggc tttaccgcga cggccgttat ccaacagcga gtcaactgct tcttgcaaca
     7321 tacgtttttc gttgcggacg ataatgtcgg gcgcgtgcag ttccagcagg cgtttcagac
     7381 ggttgttacg gttgatgacg cgacggtaca aatcgttcaa atcggaagtg gcaaaacgac
     7441 cgccatccaa tggaaccaac ggacgcaaat caggcggcaa taccggcagc acgtccataa
     7501 tcatccattc cagcttcata ccggaacggt ggaaggcttc caatactttc aaacgtttgg
     7561 cgattttttt gattttggtg tcggatccgg tcgattccaa ctcttggcgc aggatttcga
     7621 tttcgcccgc tacattcagg gtacgcagca attcgcggat accttccgca cccattttgg
     7681 catcgaaatc gtcgccgtat tcgtccagct tgttgtagta atcgtcttca gtcagcaatt
     7741 ggcggcgttg cagcggagtc ataccgggat cggttaccac aaatgcttca aagtacaata
     7801 cgcgctcgat atcgcgcaaa gtcatgtcca gtaccatacc caagcgggaa ggcagggatt
     7861 ttaagaacca aatatgtgcg acgggcgcag ccaattcgat atggcccatg cgttcgcggc
     7921 gcactttgga cagggttact tccacgccgc atttttcaca cgttacgcct ttaaatttca
     7981 agcgtttgta ttttccgcac aagcattcgt agtctttgac cgggccaaag attttggcac
     8041 agaacaaacc gtcacgctca ggtttgaacg tacggtagtt gatggtttca ggttttttga
     8101 cttcgccata agaccatgag cggatggttt cgggagaggc aataccgatt ttaatggcat
     8161 caaactcttc ttccatgccg gcagtttgca acggattaaa taagttcaac aaattcattt
     8221 ttgctccttg aaggaagtat ttttaccgca tggcggattt tcgatatggg gaaacggtac
     8281 ttttgatttc agacggccta aatgccgatt gcttcattct tattctgaaa ccactttttc
     8341 agacgaccct gaaaggccgt ctgaaaactt ttgtttagta acgttccaaa tcgatatcca
     8401 agcccagtga gcgaatctct ttgaccaata cgttgaagga ctcgggcata ccggcatcga
     8461 ttttgtgttc gcctttgacg atgttttcgt acattttggt acggccgttc acgtcgtcag
     8521 acttcacagt cagcatctct tgcagcgtgt atgccgcgcc gtatgcttcc aatgcccaaa
     8581 cctccatctc accgaaacgt tggccgccga actgggcttt accgcccaaa ggctgctggg
     8641 taaccagact gtacggaccg gtagaacgcg cgtgcatttt ttcgtcaacc aagtggtgca
     8701 gtttcagata gtgcatcaca cctactgtaa ccttgcggtc aaatgcttcg cctgaacggc
     8761 cgtcatacag cgtgatttgg gttttactgt cgttgaagcc cagtttttca acctcaggat
     8821 cgtcgctcgg ataagccaag ttcaacattt cgcggatttc agactctttc gcaccgtcga
     8881 atacaggaga ggcgaaagat gcacctttac gcaggttgga ggccagttcg atgatttctt
     8941 catcagtcag ggcatccaaa tcttctttct tgccgctacc gttgtagagc ttgttcaaga
     9001 attcacgcag ctcgctggct ttgcgttgct ctttcagcat acggtcgata cgctcgccga
     9061 taccttttgc tgcccaaccc aagtgaactt ccaaaatctg accgatgttc atacgggaag
     9121 gtacgcccaa tgggttcagt acgatgtcta ccggacggcc gtccgccatg taaggcatgt
     9181 cttccactgg cagaatgcgc gataccacgc ctttgttacc gtggcggccc gccattttgt
     9241 cgccggcttg cagacggcgt ttgatggcga taaaaacttt caccattttt tgtacgccgg
     9301 gttgcaattc gtcgccttgg gtcagttttt tcttcttgat ttcgtataac tcgtccgctt
     9361 cttcgcgttt ttgttgcagg ctcaatttaa tcagttccaa ctgtttggcc aaatcttcat
     9421 cagccagacg gatatcgaac caatcgtgcc tgctcggcag acccgccaga tattcggtcg
     9481 tgatttcgct gcctttggcc agcttcatcg gaccgccgtt ggctttctga ccgacaatca
     9541 tacgctcgat acggtcgaat gcgtcgttgt cgaaaatacg caattggtcg ttcaaatcca
     9601 aacggtagcg tttcaactcg gaatcgataa tggattgggc gcgtttgtcg cgttggatgc
     9661 cttcgcgggt aaaaacttga acgtcgataa ccgtaccgct cataccggta ggcatacgca
     9721 atgaagtatc ttttacgtca gatgcttttt caccgaagat ggcgcgcagc agtttttctt
     9781 ccggcgtcag ctgggtttcg cctttaggcg ttactttgcc taccaacaca tcgccggctt
     9841 ctacttccgc accgatataa acgataccgg attcgtccaa acggttttgc atacgctcgg
     9901 acaagttcgg aatatcgcgg gtaatgtctt ccgcacccag cttggtatcg cgggcaacga
     9961 cattcaattc ctcaatgtga atcgaagtat agcggtcgtc cgcagccact ttttcggaaa
    10021 tcagaatcga gtcttcgtag ttgtaaccgt tccacggcat gaaggcgatg gtcatatttt
    10081 gacccaaagc caattcgccc aaatcggtgg acgcgccgtc ggccaccaaa tcgccgcgct
    10141 gcaaaacatc gccggctttg acggcaggac gctgattgat attggtagac tggttggaac
    10201 gggtaaattt aaccaagttg taaatatcga cacccacttc gccggcagtc gcttcgtcgt
    10261 catggacacg aatcacaaca cggttggcat cgacatactc gaccacgccg cctcggcggg
    10321 caacgattgc agtagcagag tcaacggcaa cggaacgctc gataccggta ccgaccatcg
    10381 gtttttcagg acgcaagcaa ggcactgcct gacgttgcat gttggcaccc atcaatgcgc
    10441 ggttcgcgtc atcatgttcc aagaacggaa tcagggatgc tgcaacggat accacttgac
    10501 cagttgccac gtccatatat tggacgcggt cgggcgttgc cataatggtt tcgccttttt
    10561 cacgacaggt aaccaaatcg ccaatcagat tgccatctga atccaaatcg gcattcgcct
    10621 gtgcaatcac atagcggcct tcttcgatgg cagacaagta atcgatttcc tcggttactt
    10681 tgccgtcgat aacgcggcgg taaggcgttt ccaagaaacc gtaatcattg gtgcgcgcat
    10741 aaacggacaa tgagttgatc aaaccgatgt tcggaccttc aggcgtttca atcggacata
    10801 cgcgaccgta gtgggtcgga tgcacgtccc gcacctcaaa tcctgcacgt tcgcgggtca
    10861 aaccgcccgg acccaatgcg gatacgcggc gtttatgggt tacttcagac aagggattgg
    10921 tctgatccat aaactgactc aattggctgg agccgaagaa ttctttaata gcggcagaaa
    10981 caggttttgc attaatcaaa tcgtgcggca tcaagttttc tgattccgcc tgattcaaac
    11041 gttcttttac ggcacgttcc acacgggcca aaccgctacg gaattggttt tcagtcagct
    11101 cgcctaccga acgtactcgg cggttgccca agtgatcgat atcgtccact tcgccatggc
    11161 cgttacgcaa ctcgaccaaa gtcgcaatcg aggcgacaat atcttcgacg ctcaggacat
    11221 aaccgccttt gtcggcagca ccggcaaacg tttcgttcag caggcggccg taccaagagt
    11281 tttgttgggc ttcggacagt ttttgttcgt atgtgcgcgt attaaatttc atacggccta
    11341 cgcgggacag atcgtagctg tcttcactga agaacaagcg gttaaacaat tgctcgaccg
    11401 cctcttcggt gggcggttcg cccggacgca tcatacggta aatcgcaaca cgagccgcct
    11461 gccggccggc agtctcatcc gtacgcaagg tattggagat ataagcaccc tgatccagct
    11521 cattgatata aagggtcgta atttctttta cgccgttgat atcaaatttg gccaacaact
    11581 cttctgtaat ttcatcattg gcagaagcca atacctcgcc ggtttccgaa tcaatcagat
    11641 cggcagccaa tgctttgccc agcaggcttt ccggttctac atccaaacgg gtcaggcctg
    11701 cattggtaat atcacggata tttttcgcag taatgcgctt acctttggca accaatacat
    11761 tgccttcttt atccaagata tcgaccttgg cagtttcgcc tttcagacgg tctgcgacca
    11821 aatcggtttg aacaccgttt gaagacaaat agaacgtttc tttgtcgtag aaaatatcca
    11881 agatttgctc attgttgtag cctaaagcct tcaacaaaat cgttaccggc attttacggc
    11941 ggcggtcgat acggaaatac agcaaatctt tcggatcaaa ttcaaaatcc aaccatgaac
    12001 cacggtaggg aatgatgcgg gcggagaata acaatttgcc ggaagagtgc gtcttacctt
    12061 tgtcatgctc gaagaatacg ccgggcgaac ggtgcaactg ggagacaatc acacgctctg
    12121 tgccgttaat cacaaaagaa ccgctcgggg tcatcaacgg aatttcgccc atatacactt
    12181 cgttttcacg aacttctttt accgtcggtt tagatgcttc cttatccaaa atcaccaaac
    12241 ggatacgcgc gcgcaagggg gctgcataag tgattccgcg caactgacat tcgggaatat
    12301 cgaacaaagg ctcgcccaat gtgtaatgca caaactccaa tcgcgcataa ccgttatggc
    12361 tcacaatcgg gaaaatagaa ttaaatgccg cctgcagacc gtcatcggta cgtttgtcaa
    12421 aagcattttc cagctgcaaa aacttcgcat aagaatcaat ttgggttgcc agcaagaaag
    12481 gaacttccaa aacattttcc cgctttgcaa aactcttacg gatacgtttt ttctcggtaa
    12541 acgaatagct catatacact ccgaaaagta attttcaata ataggccgtc tgaaagcaac
    12601 ggcacgcatc aatctgatgt ttacatttat ttgcaatgat ttgataaaaa ctatgcaaat
    12661 aaatgtaaac aatacttaat gcttatttta agaagcaaaa taaggctggc agaaaactgc
    12721 cagccttcat aagaagcatc aaattatttg atttcgactt tagcgccggc ttcttccagt
    12781 tgtttttgga tgtcttcggc ttcagcttta gaaacaccct ctttaatggt tttaggtgca
    12841 ccgtcaacga tgtctttagc ttctttcaga cccaaaccgg taattgcacg gacaacttta
    12901 atcacgccga ctttttgatc accggcagaa gccaaaacga catcaaattc ggttttttct
    12961 tcagcatcgg cagcaccggc accagcagga cctgcaactg caacagcagc agcagaaaca
    13021 ccgaattttt cttcaaaagc tttaaccaag tcgttcaatt ccattacggt caaagaacca
    13081 actgcttcca aaatgtcttc tttagtaata gccatgctat tttactccaa atattgtatt
    13141 aaaaaataat tgattaacag aaacaaaatc gattaagcgg cttcttcgcc ggcttttttc
    13201 tctgccaaag cagccaaacc gcgcgcaaag cccgatacag gagcttgcat aacgaacaac
    13261 agtttggaca acagctcttc gcggctcgga atagaagcca actcagcaac ctgagcagca
    13321 ttcattactt cgccattgta agaaccggct ttaacgacaa ttttgtcatc ttttttcgcg
    13381 aattggtgca acactttagc agcagcaaca gcatcttcag aagcagcgta aaccaacgga
    13441 ccaaccattt gatcggccaa ttctgcaaat gaagtacctt gcactgcacg acgagccaaa
    13501 gtatttttca gaacgcgcaa ataaacgcct tctttacgtg cattcgcacg aagctcagtc
    13561 atactggaaa cactgatacc gcgatattca gcgactacga gggtttgagc attagcaatt
    13621 gccgcgctaa tttcctcgac cgccactttc ttggtttcaa tattgagact caaggtctac
    13681 ctcccactgt ttataaacag gataccaaaa cgatatccca ccagcgcggt aacctaaaaa
    13741 gacatcttac aagcaatctt gcaatgtgtt tcaggactac cgtctgcgta gggcagttac
    13801 gattaaatct tacgatccct acggtcttgg acatctactt aattttaagt agcccaaatt
    13861 caagcagccc agaaaaccaa actgcttgaa aattccttaa gattagttat ttacgcttga
    13921 tgtatcaacg cgaataccca aacccatggt gctagacaca gcaacttttt tcagatactg
    13981 acctttagcg gcagcaggct tggctttaac gatagcatcc agcaacgcat caaagttctc
    14041 tttcaaatca gcttcagcga atgaagcacg accaatcgtt gcatgaacaa tacctgcctt
    14101 gtcggtacgg tactgcactt gacctgcttt tgcatttttt actgcttcgg caacattagg
    14161 ggtaaccgta cctactttag ggtttggcat cagaccacga ggacccaaga tggtacccaa
    14221 ttgaccaaca atacgcattg catcgggaga agcaataaca acgtcaaagt tcagattgcc
    14281 tgctttgatt tcagcagcca aatcttcgaa accgacgata tctgcaccag cttctttagc
    14341 agcttctgca tttgcacctt gagtaaatac agccacacgg gttatcttac cggtgccttt
    14401 aggcagaacg accgaaccac ggataacttg gtcagattta cgcggatcaa cgcccaagtt
    14461 gaaagatacg tcaacagact cgtcaaattt agcagtcgct gcttttttta ccaaagcaat
    14521 tgcttcatca attgcatata atttattggc ttccacagaa gagcgaagag ctttcaagcg
    14581 tttagatact ttagccatta tacaacaccc tccacatcca agcccattga gcgagcagaa
    14641 cctgctatag tacggacagc cgcatccaag tcagcagcag tcaaatcagg atctttagtt
    14701 ttagcaattt cttccaactg ggcacgggtc aatttaccca ctttgttggt cagaggatta
    14761 gaactacctt tttgcaaacc ggcagccttt ttcaacaaga tagaagctgg cggggttttc
    14821 atcaaaaatg tgaatgattt atctgcaaat gcagtaatca caaccggaat cggtaagcca
    14881 ggctccatac cttgggttgc agcattaaat gccttacaaa attccataat attcaaaccg
    14941 cgctgaccca aagcaggacc aaccggagga gatggattgg ctttacctgc aggaatttgc
    15001 agtttaatat agccgataat tttctttgcc acttaacgaa ctcctaaaaa cgggtaagac
    15061 gcggaaccat ttccgctccc caagttaatt tttgattcta tcctcttatt gctttttttt
    15121 caagtataaa aatcagttaa tcttttcaac ctggctgaac tccagctcaa cgggtgtttc
    15181 tctaccaaat atctgaacag acacgcgtaa cttattccgt tcataattga cctcctcaac
    15241 caccccgtta aaatccgcaa acggcccttc atttacacga acctgttgac cgacctcaaa
    15301 ttcaactttt ggtttcggct tctctatgcc ggtctgaacc tgctgtaaaa taatttcagc
    15361 ctctctctga ctaatcggcg taggtctatt agccctccct ccaataaaac cggaaacacg
    15421 gggggtgctt tttacaagat gccaagagtc atctgtcatt tccatctcaa ctagcacata
    15481 accaggatat gactttcttt cactaatagt cttacgacca ttgcggatat caacaacttt
    15541 ctctacaggc accagaattt gtccgaaata atctcccatc tcctcacggg caatgcgctc
    15601 ttccaatatt cgttggacat tcttctcaaa ccccgaatac gcctgtacaa cataccattt
    15661 tttcgacatc tcaaccttcc cttctcagca atacatcaaa aaataaccac gaaattgctg
    15721 tatctgccgc atagataaat atagaaagca cagcaacaaa cactataaca aatacagtca
    15781 ttctgacagc atcttcacgc ttaggccaaa ccaccttttt gaattcggac caagaatttg
    15841 agaaatatgc aaaaaaccct tccttaccgg aattagatgc agattcttta tcttgaacaa
    15901 ccagttgatc cactttaacg ttcttttttt caggcgtatg ttctgtcata ttatttatcc
    15961 atcatagcat ctgtctattc tcaatccatg taaatggcaa gagagtttac taaataacaa
    16021 atacaaaaaa aaattaaccg gcacaaggcc ggttaatttt ttatttggca ggccaagagg
    16081 gtctcgaacc cccaaccctc ggttttggag accgatactc taccaattga gctattggcc
    16141 tctaaactta agcgataaca gaagaaacca cgccggcacc cacggtacgg ccgccttcgc
    16201 gaatcgcaaa gcgcaaacct tcttccatag cgataggcgc aatcagttct acggt
//
LOCUS       DAXHTY010000041        16113 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXHTY010000041 DAXHTY010000000
VERSION     DAXHTY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.041"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(339..761)
                     /locus_tag="ACFNMK_001097"
     CDS             complement(339..761)
                     /locus_tag="ACFNMK_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC6364381.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(879..1550)
                     /locus_tag="ACFNMK_001098"
     CDS             complement(879..1550)
                     /locus_tag="ACFNMK_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC6364382.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVWRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(1566..2192)
                     /gene="purN"
                     /locus_tag="ACFNMK_001099"
     CDS             complement(1566..2192)
                     /gene="purN"
                     /locus_tag="ACFNMK_001099"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC6364383.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCARYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2299..3117)
                     /locus_tag="ACFNMK_001100"
     CDS             complement(2299..3117)
                     /locus_tag="ACFNMK_001100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6364384.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3376..3816)
                     /locus_tag="ACFNMK_001101"
     CDS             complement(3376..3816)
                     /locus_tag="ACFNMK_001101"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC6364385.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3879..5285)
                     /locus_tag="ACFNMK_001102"
     CDS             complement(3879..5285)
                     /locus_tag="ACFNMK_001102"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC6364386.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     TFADTKIPTGNGLQAVAVVRLEKTDRAALNKAAAEAAKWAQNQETVNVDVHAFEEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFAEAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHVKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5455..6570
                     /gene="lptF"
                     /locus_tag="ACFNMK_001103"
     CDS             5455..6570
                     /gene="lptF"
                     /locus_tag="ACFNMK_001103"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC6364387.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6567..7637
                     /gene="lptG"
                     /locus_tag="ACFNMK_001104"
     CDS             6567..7637
                     /gene="lptG"
                     /locus_tag="ACFNMK_001104"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC6364388.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRFFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7947..10532
                     /gene="acnB"
                     /locus_tag="ACFNMK_001105"
     CDS             7947..10532
                     /gene="acnB"
                     /locus_tag="ACFNMK_001105"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC6364389.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10674..11669
                     /locus_tag="ACFNMK_001106"
     CDS             10674..11669
                     /locus_tag="ACFNMK_001106"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC6364390.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(11893..12906)
                     /gene="ilvC"
                     /locus_tag="ACFNMK_001107"
     CDS             complement(11893..12906)
                     /gene="ilvC"
                     /locus_tag="ACFNMK_001107"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC6364391.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12986..13279)
                     /locus_tag="ACFNMK_001108"
     CDS             complement(12986..13279)
                     /locus_tag="ACFNMK_001108"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC6364392.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            complement(13346..13837)
                     /gene="ilvN"
                     /locus_tag="ACFNMK_001109"
     CDS             complement(13346..13837)
                     /gene="ilvN"
                     /locus_tag="ACFNMK_001109"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC6364393.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(13848..15575)
                     /gene="ilvB"
                     /locus_tag="ACFNMK_001110"
     CDS             complement(13848..15575)
                     /gene="ilvB"
                     /locus_tag="ACFNMK_001110"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC6364394.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQEFMPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
BASE COUNT         3776 a         4360 c         4095 g         3882 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataatt caataaatta atatatggct taaaataacg
       61 ggattctcgc ctcccgcccg cccgcagaag caggcgggta tcattttaaa acgcggcatt
      121 taaaatttga ccgaaaattg ttgacaatcc ggaatcaagt ctgcacaata ccccgacaag
      181 tccaagtatt ataaaggctg aataaagagg aaacagcagg cagatatatt cgggaggtgc
      241 agtccgaata tatctgcttt tttatgcgcc tccggattgc ctgccgcacc tttcccttca
      301 gacggtatca gccgtttccc cataatgccg cccgatgcct atttatctgc cccggcaatt
      361 tcaaaactgt gggtaatctt tgccgctttg cccaacataa tcgaagccga acagtatttt
      421 tcggcagaca tctgaacggc gcgctcaatg gccgattctt tcaaatcatg cccgaatact
      481 ttgaaatgga tgtggatttc ggtaaacacg cgcggcgcat cgtccgcccg tttcgccgta
      541 accgtcgcac ggcagtcagt cactttctga cgctgttttt cggcaatcat caccacatcg
      601 atgctcgaac agcccgccac gcccaacagc agcatttcca aagggctggg cccgcgcttg
      661 gccgcacctt ctgccgccga accctccata acgacgctgt gcccgccttc cgtcgtgccg
      721 acaaagcaca ttccgtctat ccattttgat gtaacctgca tggtgtcatt cctgaaaata
      781 gcgttaaaac cgctttgcat atggcgttat tgtaaacaat ttcaagcggc ttatgcagaa
      841 atatggacaa aacggcaaaa aaacacttga aaaccgattt acggtttggc tgcctggccg
      901 ttgatctgca ccgatttgag tttcagcgta taggttttgc cgtcgtcggt atagccgatt
      961 tgtgccggaa tattgttcag ggacggtgcg aagaaataca ttaccgtatc gtcgccgcgc
     1021 cacacccgat atttgacgac ttcggtttcc acgccgccta tgctgtattt tcctgtaccc
     1081 gccttattca aaccgccgac ggaataaagt tttttgccgt tggtgatttt cagccccggg
     1141 gggagtttcg cgtcatttgc cgccaactgc caggcaagcg tgaacaaatc catagccttg
     1201 gggctttgct cggttttgct ctcgcccgct ttgccgtagg ttacgctgcc gtcggcgaat
     1261 ttggcttccg catacagttt gcccctgcgt atgtctctat agtaggtagg gtgcagggta
     1321 ttgccgacaa ccgtaccgcc ggactcgaaa cggatattgt atagcggcac tttaatcgtc
     1381 gaaacgattt tgtaagcatt gccgctgcgt tcaaatgtca tcgtggcggg aatgccgtag
     1441 ctgccggaat agtgcagcac ggcggattgg ggcagccctg ccgcatacgc gcacggcagg
     1501 gcggcggaca aaatggcggc ggaaaatata tttttaaaag tcttcatcat ttgctcccgc
     1561 ccggtttacg ccgtcaggaa acgggcggca tcggcgtttt ccgaatttct gacgcggttt
     1621 ccctcgataa tcaggcggcc ggcggcaaaa tcggcaacgg ctttcggata aagtttatgc
     1681 tcgacagcca aaacccgtgc ggcaatatcg tctgccgtat cgccgtcaag tatcggcaca
     1741 atcccttgcg atacaatcgg gccgcaatcc agttcggcag taacgaaatg gatggtgcag
     1801 ccggcaacgc ggcagcccgc ctccaaagcg cgttcgtgcg tatgaagtcc ggtaaacgag
     1861 ggcaggatgg acgggtgaat gttcatcagc ctgccttcgt aacgggcgca aaactcgggg
     1921 gtcagaatcc gcataaaacc tgccaaaacc accaagtcgg gttgatatgc gtcgattttt
     1981 tccatcatgg ccgtatcgaa ggcaagtcgt gattcaaagt ttttatgatt caggctatcg
     2041 gtcgggatgc cgcgttcggc cgcccattgc aaaccggcag ccgtttcgct gttgctcaac
     2101 acggcggcaa tgcggacgtt gggaatggcg gcattgacga ttgcctgcat attgctgccg
     2161 cgtccggaaa tcaggatgac gatgtttttc ataatggtac gcttttgaaa gggatgccgt
     2221 ctgaaccgct gtttggtggt ttcagacggc atttgccgta aaaatgcccg aaaacctgtt
     2281 tcgggcatgg attcggactt aatttacttt tttgatgtcg acttgagccg gctgcttggc
     2341 gggcgcgttt tcgggtgcgc cgattttgac cagtttcaca tcaaatacca aagtggcgtt
     2401 cgggccgatt ttgtcgcccg caccctgttc gcggtaggca aggttggacg ggatgtagaa
     2461 cgtggcttcg ccgccttctt tcagaagctg tacgccttcg gtccaacccg gaatcacttg
     2521 gctcaaaggg aaggtgaccg ggccgccgtt ggctttgctg ctgtcgaata ccgtaccgtc
     2581 aatcaggcgg ccttcgtatt ccacggtaac gatgtcgtct ttggtcggct gtttgccttc
     2641 gccctgtttg gtgattttgt attgcaggcc ggaagcagtg gtcttcacgc cgtctttggc
     2701 ggcattttct ttcagaaagg cttcgccttt ttctttattg gccttcgcgt ccgccttgtg
     2761 tttttctacg gctttagcct gttgttcctg aaggaatttc atcatgactt cctgagcctg
     2821 ctcttcggtc attttgattt ctttgccgtc atacactgcc tgcatggctt cggtaaagac
     2881 tttcaaatcg atttccgcgc cctgttcctt catttgcttc agggagcgtc cgatgtccac
     2941 gcccatcgca tagcttgcct gctgcatcgt gctgccgatc gaagaggtgt cgccctgcgc
     3001 ggaagaagcg gcggcaggtt cggatgcaga tgcgggggcg gcttcttttt tgccgcaggc
     3061 ggaaagtgcc aaagcggcgg aaagggtcag tgcgctgatt ttgaaaatgg tgttcatgat
     3121 ggatcttcgc tgtcgataag gtcggaaaaa cgggattata gccgagtttg aatgtttcaa
     3181 cacacaggat gacacataaa gcgtcaatcg tgtgttgccc tgttttggaa aggattgaac
     3241 cttccaaaat aagttttgat tctaccgccc cgagggacag atgtccaagt ggcggggttc
     3301 aaccgataag gaaattttaa tcaaatagaa tcaagcctgt ttaaattttg taaatgcggc
     3361 atttcagacg gcattttatg ccttgccctc catgccgtga tgttcgatgg caaaaccgct
     3421 tcggcggtag gcggtaaagc gttcgcgcgc gtcggcgaga tcttccaagc tgttgccgac
     3481 gatttccaaa acgcgcgcgg gcaagaccgg agcggtgttc caaaaaccgt cggacaggtt
     3541 caaaacggtc atgccttcgg gaattcgggg cagattgccg ccgcaggcaa gcaggacgga
     3601 tggttcagac ggcatggctt cttccgtttc ccaaatttcg tgcggaatga aactctcggc
     3661 ctcgtattgc caaagcattt tgtccaattc ctgaagctgc ccgaacgaat cggaccacac
     3721 cagtatcctg ccgccgtccc gaatcgcacg ggcaatcagg cggcaggtga aaatcggaac
     3781 ttgggcaacg tgcgtgtaaa aggtggcttt cggcatattg tttgagcatt tggcaggacg
     3841 atgccgtctg aaaggcttca gacggcattg tggcaaaatt aaagattccg cagatagttc
     3901 agcagcaagg gaacgggacg gccggtcgca cctttttccg caccggattt ccacgccgta
     3961 cccgcgatgt caaggtgtgc ccatggatag tcttcggtaa agtaggatag gaatgttgcg
     4021 gcggtaatcg tgcccgcgcc gggcgtgccg atgtttggaa tgtcggcaaa gttggatttg
     4081 agttggtctt tgtaggtctc aaagagcggc agttgccacg ctttgtcgtc cacgttgtag
     4141 gaagcggcaa gcaggctgtc gaccaaatct tgattgttgc ccatcacgcc gctgacatcg
     4201 tgccccaagg caacaataca cgcaccggtc agggtggcga cgtcgatgac ggctttgggt
     4261 ttgaactgtt cggcgtaagt gagcgcgtcg cacaaaatca gacggccttc agcatcggtg
     4321 ttcaacactt cgatggtcag gcctttcatg ctttttacga cgtcgcccgg tttgttggcc
     4381 gcgccggaag gcatattttc acaagtggcg acgatggcaa tcaggttgat cggcagttgc
     4441 agtttgacgg cggcgcagaa ggtgctgatg acggttgccg cgccgcacat atcaaacttc
     4501 atttcgtcca tgttcagacc gggtttgagg gaaatgccgc cggtgtcgaa ggtaatgcct
     4561 ttgccgacca agacgacggg ggcggcttct ttgtcggctg caccgaaata gctcagttcg
     4621 accaaatagg gatcttcgac actgcctttg gcgaccgacc aaaacgaacc catgttttct
     4681 ttgatgtagt ctttttcgat gattttgacg tgcgcgccca gtttttcggc ttcggctttg
     4741 gcggtgcgcg ctaaaaattc gggcgtgcat tcgttcggcg cggcattgcc caagtcgcgg
     4801 cagaggcttt gtccgtaaac ctgtgcttcg gcaacgcgca gggcttcttt gacggcggct
     4861 tcgtgtgcgc tgtggaacac ggcttctgca aatttggcgg gcttggcttc ttttttatag
     4921 cggtcgaaac ggtaggcggc attgccgaaa gcaatcgcaa acgcttcggc aacggctgcg
     4981 gcttgcgctt cttcaaaggc atgaacgtcc acattgaccg tttcctgatt ttgcgcccat
     5041 ttggcggctt cggcagcggc tttgttcagc gcggcacggt cggttttttc aaggcggacg
     5101 acggcgactg cctgcaaacc gttgcctgtc gggattttgg tgtcggcaaa agtttgacct
     5161 tcttcaagcg aagacaaaag ggcaagggcg gtcggatggt ttaattgcgc ctcttcggtg
     5221 cagacaaata actgtgcgcc tgcctgctgt tcctgcaaga tttcggtttt tgtgctaaat
     5281 tccacgttta ttctcctgat tgagacggtt gtcggtagtt ttcggacggc ctttcgctca
     5341 aaagaccgtc tgaagacggc tggcacgatt gtaccccatt tgaagcaccg tctgaaacct
     5401 tgcgcggaca atccgcctgc gccgaaccgc ttaccgcccc ctgaccgcga ttctatgatt
     5461 tatcaaagaa acctcatcaa agaactctct tttaccgccg tcggcatttt cgtcgtcctc
     5521 ttggcggtat tggtctccac gcaggcaatc aacctgctcg gccgtgccgc cgacgggcgt
     5581 gtcgccatcg atgccgtgtt ggcattggtc ggcttctggg tcatcggtat gacgccgctt
     5641 ttgctggtgt tgaccgcatt tatcagtacg ttgaccgtgt tgacccgcta ctggcgcgac
     5701 agcgaaatgt cggtctggct atcctgcgga ttggcattga aacaatggat acgcccagtg
     5761 atgcagtttg ccgtgccgtt tgccgttttg gttgccgtca tgcagctttg ggtgataccg
     5821 tgggcagagc tacgcagccg cgaatacgct gaaatcctga agcagaagca ggaattgtct
     5881 ttggtggagg caggcgggtt caacagtttg ggcaagcgca acggcagggt ttattttgtc
     5941 gaaaccttcg ataccgaatc cggcatcatg aaaaacctgt tcctgcgcga acaggacaaa
     6001 aacggcggcg acaacatcat cttcgccaaa gaaggtaact tctcgctgaa cgacaacaaa
     6061 cgcacgctcg aattgcgcca cggctaccgt tacagcggca cgcccggacg cgccgactac
     6121 aatcaggttt ccttccaaaa actcaacctg attatcagca ccacgccaaa actcatcgac
     6181 cccgtttccc accgccgtac cattccgacc gcccaactga ttggcagcag caacccgcaa
     6241 catcaggcgg aattgatgtg gcgcatctcg ctgaccgtca gcgtcctcct actctgcctg
     6301 cttgccgtgc cgctttccta tttcaacccg cgcagcggac atacctacaa tatcttgatt
     6361 gccatcggtt tgtttttaat ttaccaaaac gggctgaccc tgctttttga agccgtggaa
     6421 gacggcaaaa tccatttttg gctcggactg ctgcctatgc acatcatcat gttcgtcatc
     6481 gcaatcgtac ttctgcgcgt ccgcagcatg cccagccagc ccttctggca ggcggttggc
     6541 aaaagtctga cattgaaagg cggaaaatga acctgatttc acgttacatc atccgtcaaa
     6601 tggcggttat ggcggtttac gcgctccttg ccttcctcgc tttgtacagc ttttttgaaa
     6661 tcctgtacga aaccggcaac ctcggcaaag gcagttacgg catatgggaa atgctgggct
     6721 acaccgccct caaaatgccc gcccgcgcct acgaactgat gcccctcgcc gtccttatcg
     6781 gcggactggt ctctctcagc cagcttgccg ccggcagcga actgaccgtc atcaaagcca
     6841 gcggcatgag caccaaaaag ctgctgttga ttctgtcgca gttcggtttt atttttgcta
     6901 ttgccaccgt cgcgctcggc gaatgggttg cgcccacact gagccaaaaa gccgaaaaca
     6961 tcaaagccgc ggccatcaac ggcaaaatca gcaccggcaa taccggcctt tggctgaaag
     7021 aaaaaaacag cattatcaat gtgcgcgaaa tgttgcccga ccataccctg ctgggcatta
     7081 aaatctgggc ccgcaacgat aaaaacgaac tggcagaggc agtggaagcc gattccgccg
     7141 ttttgaacag cgacggcagt tggcagttga aaaacatccg ccgcagcacg cttggcgaag
     7201 acaaagtcga ggtctctatt gcggctgaag aaaactggcc gatttccgtc aaacgcaacc
     7261 tgatggacgt attgctcgtc aaacccgacc aaatgtccgt cggcgaactg accacctaca
     7321 tccgccacct ccaaaacaac agccaaaaca cccgaatcta cgccatcgca tggtggcgca
     7381 aattggttta ccccgccgca gcctgggtga tggcgctcgt cgcctttgcc tttaccccgc
     7441 aaaccacccg ccacggcaat atgggcttaa aactcttcgg cggcatctgt ctcggattgc
     7501 tgttccacct tgccggacgg ttcttcgggt ttaccagcca actctacggc atcccgccct
     7561 tcctcgccgg cgcactgcct accatagcct tcgccttgct cgccgtttgg ctgatacgca
     7621 aacaggaaaa acgctaaacc aatgccgtct gacccccttc ggacggcatt ggtttttatt
     7681 atcatattcc tgtagacaga tagccgttcc ctattatagt ggattaacaa aaaccagtac
     7741 ggcgttgcct cgccttgccg tactattgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     7801 ttttgttaat ccactataca ttacctgtca taacagttcc atttttgtta aaactagtct
     7861 atgatagcgg tacaaatatt gtttacaata tttaacgcaa atcatttgca acccgacaaa
     7921 agaaaaacag aaaaaggaac aaagagatgt tagaagccta tcgtaaagcc gccgccgagc
     7981 gcgccgccct cggcattccc gccctccctt tgaacgcgca gcaaaccgcc gatttggttg
     8041 agctgctgaa aagcccgccc gcaggcgaag gcgagttctt ggtcgaactg cttgcccacc
     8101 gtgttccgcc cggtgtggac gatgccgcca aagtcaaagc ctcattcctg gctgccgttg
     8161 ccgaaggcag cgcgtccagc ccgctgatct cccccgaata tgcgaccgaa ctcttaggta
     8221 cgatgctcgg cggttacaat atccacgcct taatcgaact cttggacgac gacaaactcg
     8281 cgcccattgc cgccaaaggc ttgaaacata cgcttctgat gttcgattcc ttccacgacg
     8341 ttcaggaaaa agccgaaaaa ggcaacaaat acgcgcaaga agttttgcaa tcttgggcag
     8401 atgccgaatg gttcgcctca cgcgccaaag ttcccgaaaa aatcaccgtt accgttttca
     8461 aagttgacgg cgaaaccaat acagacgacc tctcccccgc gcccgacgcg tggagtcgtc
     8521 ccgatattcc gctgcacgcg ctggcaatgc tgaaaaaccc gcgcgacggc atcacgcccg
     8581 acaaaccggg cgaagtcggt ccgattaaat tgttggaaga actcaaagcc aaaggccatc
     8641 cggttgctta cgtcggcgac gtggtcggta ctggttcttc acgcaaatcc gcgaccaact
     8701 ccgtcatttg gcataccggc gaagacattc cgttcgtgcc gaacaaacgc ttcggcggcg
     8761 tatgtttggg cggcaaaatc gcgccgattt tcttcaatac ccaagaagat tccggcgcgc
     8821 tgccgattga agtcgatgta tctgctctaa aaatgggcga tgtcgtcgat atcctgcctt
     8881 atgaaggcaa aatcgtgaaa aacggcgaga ctgttgccga gtttgaattg aaatcacaag
     8941 tattgctgga cgaagtgcaa gccggcggcc gtatcaacct gattatcggc cgaggtctga
     9001 ccgccaaagc gcgcgaagcc ctgaaactgc ctgcctctac tgcattccgc ctgccgcaag
     9061 cgcctgccga aagcaaagcc ggtttcacct tggcgcaaaa aatggtcggc cgcgcctgcg
     9121 gtctgcccga aggacaaggc gtgcgcccgg gtacttactg cgaaccgcgt atgacgacgg
     9181 tcggctcgca agacacgacc ggcccgatga cccgcgacga gttgaaagac ttggcttgtt
     9241 tgggcttctc cgccgatatg gtgatgcagt ctttctgcca caccgccgcc tatccgaaac
     9301 ctgtcgatgt aaaaacccat aaagaactgc ccgcctttat ttccacccgt ggcggcgtgt
     9361 cactgcgtcc gggcgacggc gtcatccact cgtggctcaa ccgcctgctg ctgcccgata
     9421 ccgtcggcac cggcggcgac agccataccc gtttccccat cggtatttcc ttccccgccg
     9481 gctccggctt ggttgccttt gccgccgcaa cgggcgtaat gccgctcgat atgcccgagt
     9541 ctgtattggt acgcttcagc ggcaagctgc aaccgggcgt aaccctgcgc gatttggtga
     9601 acgccatccc gctgtacgca atcaaacaag gtttgctgac cgttgccaaa gccggtaaga
     9661 aaaacatctt ctccggccgc atcctcgaaa tcgaaggcct gcctgatttg aaagtggaac
     9721 aagcctttga attgaccgac gcatccgccg aacgctccgc cgccggctgt accgtgaagc
     9781 tcaacaaaga gccgattatc gagtacatga aatccaacgt cgtgttgatg aaaaacatga
     9841 ttgccaacgg ctatcaagac ccgcgcactt tggaacgccg catcaaagct atggaaaaat
     9901 ggctggcaaa tcccgagttg ctcgaagcgg ataaagatgc cgaatacgcc gccgtgattg
     9961 aaatcaacat ggacgacatc aaagagccga ttatcgcctg cccgaacgac ccggacgacg
    10021 tgtgcttcat gtccgaacgc tccggcacca aaatcgacga agtattcatc ggttcgtgta
    10081 tgaccaacat cggccacttc cgcgccgcct ccaaactttt ggaaggcaag gcagacaccc
    10141 ccgtccgcct gtggattgcg ccgccgacca aaatggacgc gaaacaattg tccgacgaag
    10201 gacactacgg cgtactcgga cgtgccggcg cgcgtatgga aatgccgggt tgctccttat
    10261 gtatgggtaa tcaggcgcaa gtacgcgaag gtgcgaccgt tatgtccacc tccacccgca
    10321 acttcccgaa ccgtttgggt aaaaacacct ttgtttacct cggttcggcg gaattggcag
    10381 cgatttgctc caaactgggt aaaatcccga ccgttgaaga atatcaagcc aatatcggca
    10441 tcatcaacga acagggcgat aaaatctacc gctatatgaa cttcaacgaa atcgacagct
    10501 acaacgaagt agccgagacc gtgaacgttt aatccccgtc atccgtatga agtaagggat
    10561 tgaccgcaat gccgtctgaa caaccttcag acggcattgc aacattccgc taacccttct
    10621 ttccgcaaac gctgcaaata cggcgttcac gcccccacat aaaggaaacg acagtgaacc
    10681 tgaaaaaccg ccattttctg aaacttttag acttcacgcc ggaagaaatc accgcctacc
    10741 tcgaccttgc cgccgaattg aaagccgcca aaaaagcagg gcgcgagatt cagcggatga
    10801 aagggaaaaa catcgccctg atttttgaaa aaacctctac tcggacgcgc tgcgcgtttg
    10861 aagtcgccgc gcgcgatcaa ggcgcgggag tgacttattt agagccgtcc gccagccaaa
    10921 tcgggcataa ggaaagcatc aaagacaccg cccgcgtgtt gggcaggatg tacgatgcca
    10981 tcgaatatcg cggtttcggt caggaagttg ttgaagaatt ggcgaaatac gcgggcgtac
    11041 ccgtgttcaa cgggctgacc aacgagttcc atcccacaca aatgcttgcc gacgcactga
    11101 ctatgcgcga acacagcggc aaacctttga accaaaccgc gtttgcctac gtcggcgacg
    11161 cgcgttacaa catgggcaat tccctgctga ttttaggggc aaaattgggg atggacgtgc
    11221 gtatcggcgc accgcaaagc ctgtggccgt ctgaaggcat tattgccgcc gcacacgccg
    11281 ccgccaaaga aaccggcgca aaaattaccc tgaccgaaaa cgcgcatgaa gccgtgaaga
    11341 atgttgattt tattcatacc gatgtgtggg tcagcatggg cgagccgaaa gaagtctggc
    11401 aggaacgcat cgatttgctg aaagattacc gcgttacgcc cgaactgatg gcggcatcgg
    11461 gcaatccgca agtcaaattc atgcactgcc tgcccgcctt ccacaaccgc gaaaccaaag
    11521 tcggcgaatg gatttacgaa accttcgggc tgaacggtgt ggaagttaca gaagaaatat
    11581 tcgaaagccc cgccagcatc gtgttcgatc aggcggaaaa ccgtatgcac acgattaaag
    11641 cggtaatggt cgcggctctg ggcgactgac agaactgtgc ctgtttaaat tcatccgcaa
    11701 cacagatacc gtctgaacac gatgttcaga cggtatccat atatagtgga ttaaatttaa
    11761 accagtacgg cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct
    11821 tgtcctgatt tttgttaatc cactataaaa aaactgccta cacgatgtgt aggtagtccc
    11881 gtttgaaaac aatcagtttt tgtcttggtc aaccaatttg ttggcagtaa tccaaggcat
    11941 catggcacgc agttgtgcgc cgactttttc aacttggtgg tcggcattca gacggcggcg
    12001 ggcagtcata gacgcatagt tgacattacc ctcttggata aacatttttg cgtattcgcc
    12061 ggtttgaatg cgtttcaggg cattgcgcat ggcttctttg ctggaagcat tgaccacttc
    12121 agggccggta acgtattcgc cgtactccgc attgttggaa atggagtagt tcatattggc
    12181 aataccgcct tcgaaaatca ggtcaacaat cagtttcatt tcgtgcagac attcgaagta
    12241 agccatttca ggcgcgtaac cggcttcggt cagggtttca aaacccgcct tgatcaactc
    12301 gaccacgccg ccgcacaata cggcttgttc gccgaacaga tcggtttcgg tttcttcgcg
    12361 gaaagtggtt tcaatcacac cgcctttggt gccgccgttg gcagccgcat aagacagggc
    12421 gatgtctttg gctttgccgg aattgtcttg gtaaacggca atcagagaag gcacgccgcc
    12481 gccgcgtttg tattcactgc gtacggtatg gcccggacct ttgggggcaa ccataatcac
    12541 gtccaagtcg gcacgcggaa cgatttggtt gtagtgcacg ttgaagccgt gtgcaaatgc
    12601 cagcgttgcg ccttctttca aattggctgc aacttcggcg tgatagacgg caggcatggt
    12661 ttcgtcaggc agcagcagca taacgacatc ggcttctttg gtcgcttcag caacggtttt
    12721 gacgacatgg ccggctgctt cggctttttt ccaagaagaa ccttggcgca gaccaatcac
    12781 cacgtttaca cccgaatctt tcaggttggc ggcatgggca tgaccttgcg aaccgtaacc
    12841 gatgatggca acggttttgc ctttgattag ggacagatcg gcatctttat cgtaatagac
    12901 ttgcatttga tttcctttaa ggtaaatggt tgtcgaagcc ttaaaatgtt gagcggcttc
    12961 ggacgggtta aacagagtgt gccgcttaat cggcaacttc attcatcaat acgatttcca
    13021 acgcttcggt tttgccgtcg acggactgga cgaaggcttg gaaatgcgcg ctggcgttat
    13081 gttcgtcaat agctgcttga gatttccaat tttccacgaa aacaaaacgg ttcggtttgc
    13141 cgatttcctg atggagatcg tagctgatgt tgccctcttc tgcacggctg gctttgacca
    13201 gttctttaaa ctgtgctgcc agtgtttctg tgtattccgg tttgacggta accagtgcga
    13261 caattttaat gttcgacata aatctctcct gccgttcgtt tttcagacgg cattcaaata
    13321 ccgtgccgtc tgaaaggtta cggcgttaaa ttttcaaaat acgctcaccg cgaccgatgc
    13381 cggccgcgcc tgtacgtacg gtttccaaaa tttgggcgcg tccgaccgtt tccaaaaagg
    13441 aatccagctt gtcggtcgag ccggtaattt caatcgtata gctgcggtcg gttacgtcga
    13501 tgatgctgcc ccggtagatt tcggtcaaac gcaaaaattc gtcgcggtct ttgccggcgg
    13561 cacggacttt taccaacatc agttctcgtt cgacaaaacg gctttcgttc aaatcgacca
    13621 ctttaatcac ttcaatcaat ttattgagtt gcttggtgat ttgttcgata acctgctcgt
    13681 cgccgtgggt aacgatggtc atacgggaca gggttttgtc ttcggtcggc gcaaccgcca
    13741 aagaatcgat attgtaatcg cgtgcagaga acaaaccgac cacgcggctc atcgcacctg
    13801 attcgttttc gatcagaaca gataagatat gtcgcatttg tctctcctta cgcctttccg
    13861 tccgcacgca tatgcggcgg aagtaccatt tcgtccaaac ctttgccgtt gccgaccata
    13921 ggcatcacat tctgtttctg gtcagtcagg aagtcgataa acaccagcct gtctttttgg
    13981 ttcaatgctt ccaacaaggc accttccaca tcagacttct tgtccacgcg gataccgata
    14041 tggccgtatg cctcggcaag tttgacgaaa tcgggcaaag aatcgaaata agtttccgac
    14101 tctcggccgc cgtaatatat ttcctgccac tggcgtacca taccgagata accgttgttc
    14161 agcgtaatga cgttaaccgg aatccgatat tggaaacagg tggacaactc ttggatgttc
    14221 atctgaatcg agccgtcgcc ggtgatacag aatacgtctt gatccggggc ggcaagtttt
    14281 gcaccaatcg cataaggcag acccacgccc atcgtaccca aaccgccgga gttgagccat
    14341 tggcgcggac gttcgaaggg ataatattga gccgcaaaca tttgatgctg ccctacatcc
    14401 gatgtgatga ttgccgaatt gccggtaatc tcggcaagct tctgaatcac atattgtggc
    14461 ttgataattt cgctgccgtt gtcaaaccac aagcaatcgc gggaacgcca ttcctctatg
    14521 gttttccacc atttgcccaa agcatcttca gacggcatga actcttgttt ttgccacagc
    14581 gcaaccatct cagacaaaac atttttcacg tcgccgacaa tcggaatgtc cgccttcacg
    14641 cgtttggcga tgctggaagg atcgacatcg atatggataa ctttcttcgc cttctcgaaa
    14701 aatttggacg gtacggaaac cacacggtcg tcaaaacgcg cacctacggc aagaacgaca
    14761 tccgcattct gcatggcaag gtttgcctcg taagtaccgt gcataccgag cataccgagg
    14821 aattggcggt cgccggaagg ataagcgccc aagcccatca gcgtacccgt gcacggagca
    14881 cccgtcattc ggacaaatcg ggtcagctct tcagaagcat tacccaacac cacgccgccg
    14941 ccaaaataga cgaccggacg tttggcagat gccaacatct gcacggcctt tttaatctga
    15001 ccgatatgcc cttgaacaac cggttgatac gaacggataa aaatgtcttc ctgaggatag
    15061 ctgaatttcg ccatcgcctg cgtaacatct ttcgggacat caaccaccac gggccccggt
    15121 cggccgcttg cggcaatttg gaacgccttt ttaatggttt ccgccaactc attgatgtcc
    15181 gtaaccagga aattgtgttt gacgcacgga cgggtaatac ccaccgtatc aacttcttgg
    15241 aacgcatccg taccaatcag ggaattgcct acctgcccgc tgatgaccac catcggaatc
    15301 gaatccgtat aggcagtagc aataccggtc agcgcattgg taacgcccgg gccggatgta
    15361 accaatgcca cgcccacctt accgctgacg cgcgcatacg catctgccgc gtgtactgcc
    15421 gcctgctcgt gacgggtcag aatgtgcttg aatttattga gttggaaaag ggcatcgtag
    15481 atttcgataa ccgcaccgcc gggataaccg aaaacgtact cgacaccttc ggctttgaga
    15541 ctctgcacta tgatttgcgc gcctgataac tgcataacga cctcttttat acggtttcaa
    15601 accaataggg acaaaccgct ttgccacagc acctgtaatg caattccacc aagcagcgat
    15661 ttagggtacg cgcattgggg gaacacggca acagacggat tatccaatca attggaaagg
    15721 aacacagagt ttgtgaaaaa gagtagaaac gataacgcaa accgacagtt caatcaagaa
    15781 aaatctttca tcttttaata ttttttgaaa gcagagaaat tattgattga ttttaaaaga
    15841 atgaaatcag gagtaccttt tttgaaagat ggaaattgtt gacagtttgt gtaggagggg
    15901 cagatgtgaa aaacccttct tcgatatcaa gaattgtaaa atttacaggg tttcatccca
    15961 ataaagactc gggatattga ttgaacttga ttttattttt gatatatcaa aaatattccc
    16021 aaccatactt cctgaaaatg gctcattgca ccggactgta ttggacggca ttgacagaac
    16081 caagagggct aacaacgact taatatattg att
//
LOCUS       DAXHTY010000042        15836 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXHTY010000042 DAXHTY010000000
VERSION     DAXHTY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15836
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.042"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            95..1113
                     /gene="hupA"
                     /locus_tag="ACFNMK_001111"
                     /pseudo
     CDS             95..1113
                     /gene="hupA"
                     /locus_tag="ACFNMK_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
     gene            1144..3573
                     /gene="hupB"
                     /locus_tag="ACFNMK_001112"
     CDS             1144..3573
                     /gene="hupB"
                     /locus_tag="ACFNMK_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC6364395.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSSSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVKIENDGYVFDPTNPSPSRYLTYKATGVARSQPDPQEWVNKSTLFKLG
                     YNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTIYSPATGDYRHRQDVSYRRRSGVEYK
                     NELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINSEVYHSFRHIRQNTAQWTA
                     DFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYDPKILTSNQAKITMLIENRS
                     KYKFAYWNNVFHLGGNDRFRLNAGIRYDKNSSSAKDNPKYTTAIRGQIPHLGSERAHA
                     GFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPELKAEKAKN
                     WELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPV
                     WQNQNRTAAWVKGIEFNGTWNLDSIGLPQGTHAGVNVSYIKGKAKQTNGQETPINALS
                     PWSAVYNLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLFD
                     LSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSPG
                     RSYNFTIEAKF"
     gene            complement(4218..5852)
                     /gene="groL"
                     /locus_tag="ACFNMK_001113"
     CDS             complement(4218..5852)
                     /gene="groL"
                     /locus_tag="ACFNMK_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC6364396.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAMPDMGGMGGMGGMM"
     gene            complement(5945..6235)
                     /locus_tag="ACFNMK_001114"
     CDS             complement(5945..6235)
                     /locus_tag="ACFNMK_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC6364397.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            6465..7118
                     /locus_tag="ACFNMK_001115"
     CDS             6465..7118
                     /locus_tag="ACFNMK_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6364398.1"
                     /translation="MKITHCKLKKKVQKELLHFFVLEVTARSATDILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEQDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSRQLKILQDWCGI"
     gene            7331..8866
                     /locus_tag="ACFNMK_001116"
     CDS             7331..8866
                     /locus_tag="ACFNMK_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6364399.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            8863..8955
                     /locus_tag="ACFNMK_001117"
     CDS             8863..8955
                     /locus_tag="ACFNMK_001117"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC6364400.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(8991..10235)
                     /gene="lysA"
                     /locus_tag="ACFNMK_001118"
     CDS             complement(8991..10235)
                     /gene="lysA"
                     /locus_tag="ACFNMK_001118"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC6364401.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDEDVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIATLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(10246..10416)
                     /locus_tag="ACFNMK_001119"
     CDS             complement(10246..10416)
                     /locus_tag="ACFNMK_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6364402.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            10487..10810
                     /gene="cyaY"
                     /locus_tag="ACFNMK_001120"
     CDS             10487..10810
                     /gene="cyaY"
                     /locus_tag="ACFNMK_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC6364403.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWVAAKSGGYHFAEQNGKWLATRDNRDFYDVLNEALSAASGEAV
                     KIAEL"
     gene            10837..11853
                     /locus_tag="ACFNMK_001121"
     CDS             10837..11853
                     /locus_tag="ACFNMK_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFC6364404.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGVLLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQDLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSDGVSENTSHKITILWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTHASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            11879..12298
                     /locus_tag="ACFNMK_001122"
     CDS             11879..12298
                     /locus_tag="ACFNMK_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC6364405.1"
                     /translation="MAQLPLYLTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCVNDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSREPT"
     gene            12320..12826
                     /gene="luxS"
                     /locus_tag="ACFNMK_001123"
     CDS             12320..12826
                     /gene="luxS"
                     /locus_tag="ACFNMK_001123"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC6364406.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            12972..15764
                     /gene="polA"
                     /locus_tag="ACFNMK_001124"
     CDS             12972..15764
                     /gene="polA"
                     /locus_tag="ACFNMK_001124"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC6364407.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVRNCLSDGIGSKLVMQVHDELVLEVVETELDFIKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
BASE COUNT         3812 a         4322 c         3995 g         3707 t
ORIGIN      
        1 atttttgcat cgacccacaa aacagttgac atcatataac atttaaactg ataatcaaaa
       61 ctattattaa cacatcatca agattgagta tgcgatgaaa tacaaagccc tgtccttact
      121 gccgcttgtc gctgcccttg ccgcctgtgc gggggggtag ccgaaccgca cgtccccgtg
      181 tccatcccca ccgccacgcc gctgaccggt gaggtaaagt taacagacga caatagcaaa
      241 atcgaaaaca tcaacaccgc caacactgga aacacatccg gcatttccat tcagcagcgg
      301 gaatataaag taaacaatta cggcgtcgaa tcaacagcaa aagcattcat cttcaaaaca
      361 cctggcggcg cgcaatacac cctttcatcc tacgccgacc ccatcgtccc cagctactcc
      421 tcccccgatt tcaaaattcc cgaccgccac gcagggcagc ggcttgccga cggcagccgc
      481 atctttatct gctgcagcga ctccggggca accaatcaag cggaaattac caaacaagat
      541 tatatgaaat tcggggcgtg gataggtccc aacggcgaaa tcgacctctt cgccggcggc
      601 ttccccgtcg gcaaaacacc ggcatcaagc agttattaca gcagcacgct cgaaaccgcc
      661 ggcaagggca aaatcactta tcaggtttgg ggcatccgcg tcagaaacgg gcaatttgtt
      721 acttcctctt atacgccgcc gaaaaacagt ggttattatt cgtccacacc taccaacacc
      781 cccgtccttt cctttatcac cgccaatttc aacagcaaca aactggcagg cgaaatcatc
      841 ggcaacagcg attacgggcc aaatgtgaaa attgaaaacg caacgattga cggactatcc
      901 ttttccggcg atgccacgtc cggcggcaaa aacggcaaat tggaaggcaa gttttttggc
      961 aagttcaaca gcacccgcag cagcgacacc ggcataggcg gcaaaatcac ttttaaagac
     1021 gaccgctccc tcgataccgt attcggcggc gtgatttatg agaaaaaact tgatgacaaa
     1081 accagtcaag ataccaacca tctcaaaaaa taataaccca tccgctttta aaaaggtttt
     1141 cttatgccca tcccctttaa acccgtattg gctgccgccg ccatcgccca agcgtttccc
     1201 gcctttgcgg cagaccccgc gccgcagtcc gcccaaacgc tgaacgaaat caccgttacc
     1261 ggcacgcaca aaacccaaaa actcggcgaa gaaaaaatcc gccgcaaaac tttagacaag
     1321 ctcttggtca acgacgaaca cgacctggtg cgctacgacc ccggcatttc cgtcgtcgaa
     1381 ggcggcaggg cgggttctaa cggctttacc atacgcggcg tggacaaaga ccgcgtcgcc
     1441 atcaacgttg acgggctggc gcaggcggaa agccgctctt ccgaagcctt ccaagagttg
     1501 ttcggcgcgt acggcaactt caacgccaac cgcaacacct ccgagccgga aaacttttcc
     1561 gaagtaacca tcaccaaagg cgcggactcg cttaaatcca gcagcggcgc attgggcggc
     1621 gcagtcaatt accaaaccaa atccgcaagc gattatgttt ccgaagacaa gccctaccat
     1681 ttggggataa agggcggcag cgtcggcaaa aacagccaaa aattcagcag catcaccgcc
     1741 gccggcaggc tctttggttt ggatgcctta ttggtttata cccgccgctt cggcaaagaa
     1801 accaaaaacc gctcgaccga gggcgatgtc aaaattgaaa acgacggata tgtctttgac
     1861 ccaaccaacc cctcgcccag cagatacctt acctacaaag ccacaggggt tgcgcgctcc
     1921 caacccgacc cgcaagaatg ggtaaacaaa agcaccctgt tcaagctggg ctacaatttc
     1981 aacgaccgca accgcatcgg ctggattttt gaagactccc gcaccgaccg ttttaccaac
     2041 gagctgtcta atttgtggac gggtacaatt tactctcccg caacgggcga ctaccgccac
     2101 cgccaagacg tgagctaccg ccgccgctcc ggcgtcgaat acaaaaacga attggaacac
     2161 ggcccgtggg acagcctcaa gctgcgctac gacaagcagc gcatcgatat gaacacttgg
     2221 acttgggaca tcccgaaaaa ttacgatttg cgcggcatca acagtgaagt ttaccattcg
     2281 ttccggcata tccgccaaaa caccgcgcaa tggactgccg attttgaaaa acaactcgac
     2341 ttttccaaag ccgtttgggc ggcgcaatac ggcttgggcg gcggcagagg ggacaatgcc
     2401 aactcggatt acagctattt cgcaaaactg tacgacccca aaatcctcac ttccaaccaa
     2461 gccaaaatca caatgctgat cgaaaaccgg tcgaaatata aatttgccta ttggaacaat
     2521 gtgtttcact tgggcggcaa cgaccgcttc cgcctgaatg cgggcatacg ctacgacaaa
     2581 aacagcagca gcgcgaaaga caatccgaaa tacaccaccg ccatccgggg gcagattccc
     2641 catttgggtt cggaacgcgc gcacgcgggc ttcagctacg gcacggggtt cgactggcgg
     2701 tttaccaagc atctgcactt gttggcaaaa tacagcaccg gcttccgcgc gccgacttcg
     2761 gacgaaacct ggctgctgtt cccacacccc gatttctacc tgaaagccaa ccccgaactg
     2821 aaagcggaaa aagccaaaaa ctgggaattg ggtctggcgg gcagcggcaa ggcgggcaac
     2881 ttcaaactct caggcttcaa aaccaaatac cgcgacttta tcgaactgac gtatatgggc
     2941 gtttcgtcag acgataaaaa caaccccaga tacgccccgc tttccgacgg tactgcattg
     3001 gtcagctcgc ccgtttggca aaaccaaaac cgcaccgccg cctgggtgaa aggcatagag
     3061 tttaacggca catggaacct cgacagcatc ggtttgccgc aaggcacgca cgcaggtgtc
     3121 aatgtcagct acatcaaagg caaggccaaa caaaccaacg gacaggaaac ccctattaac
     3181 gccctttcgc catggagtgc ggtttacaat ctgggctacg acgctccttc caaacgttgg
     3241 ggcatcaacg cctacgccac gcgcaccgcc gccaaaaagc cgtccgacac cgtccacagc
     3301 aacgacgact tgaacaaccc gtggccttat gccaaacaca gcaaggccta tacgctgttc
     3361 gacctttccg cctacctcaa catcggcaaa caggttacgc tccgcgccgc cgcgtacaac
     3421 attaccaaca agcagtacta cacttgggaa tctttacgca gcatccgcga gttcggcacg
     3481 gtcaaccgcg ttgacaacaa aacccacgcc ggcatccaac gctttacctc gccgggcagg
     3541 agctacaatt tcaccatcga agcgaagttc taagctcctg atgtgccaat gccgtctgaa
     3601 acccgatgat ttgggtttca gacggcattt tttacggtcg caccgccgcc ccctttcagg
     3661 cttgtaccaa gcccaatccc gcaactctcc gtcattcccg caactctccg tcattcccgc
     3721 aactctccgt cattcccgca actctccgtc attcccgcga ctctccgtca ttcccacgga
     3781 agtgggaatc tagaaacgca aagctgcaag aatttatcgg aaatgactga aactcaacga
     3841 acctggattc ccgcctgcgc gggaatgacg aagctatccg cacagaaacc tgcaccacgt
     3901 cattcccacg aaagtgggaa tccagaacgt aaaatctgaa gaaaccgttt tatccgatac
     3961 gtttccgcac cgacagacct agattcccgc tttcgcggga atgacggcgg aaaggttgct
     4021 gtttttccga taaattcctg ccgctcttcg tttttgggat ggcgggaaat aaaacaaaag
     4081 cgcgcgtatc aaaaaataac aatgcaaaga acggtgttgg caattttaag gtttgcgcct
     4141 tagccgcact tattcgcaaa aaaaccgcac ggcgttgacc gtgcggcttg ttgtctgaag
     4201 gcttcagacg gcattgctta catcatgccg cccataccac ccatgccgcc catatcaggc
     4261 atagccggtt tgtcttcagg gatttcagca atcatgcagt ctgtggtcag catcaggccg
     4321 gcgatagacg cggcgtgttg cagcgcggaa cgggttactt tggcggggtc gagtacgccc
     4381 atttcgatca tgtcgccgta ttcgccgctg ccagcgttgt aaccatagtt gcctttgcct
     4441 tccaacactt tgttcacaac cacgctgggt tcgccgcctg cgttggcaac gatttggcgc
     4501 agcggagact caacggcgcg caagacgatt tgtacgcctg cgtcttggtc tgcattgccg
     4561 gtgtgcaggt tttccagagc ggcacgggcg cgcaacaggg ctacgccgcc gcctgcaacc
     4621 acgccttctt caacggctgc gcgggtagcg tgcagcgcgt cttccacgcg gtcttttttc
     4681 tctttcattt ccacttcggt cgcggcaccg actttgatta ctgccacgcc gcctgccagt
     4741 ttggcaacgc gctcttgcag tttttctttg tcgtaatcgc tggttgcggt ttcgatttgt
     4801 tggcggattt cggcaacacg cgcttcgatt tgggctgcgt cgccgaagcc gtcgatgatg
     4861 gtggtgtttt ctttaccgat ttcgatgcgt ttggcctgac ccaagtcgtc caaagtcgct
     4921 ttttccaaag acaggccgac ttcttcggaa atcactgtgc cgccggtcag gatagcgatg
     4981 tcttgcagca tcgctttgcg gcggtcgccg aagcccggag ctttaacggc aacggttttc
     5041 agaatgccgc ggatgttgtt cacgaccaaa gtcgccaagg cttcgccttc tacgtcttca
     5101 gcgataatca acagcggacg gctggctttg gccacttgtt ccaaaacagg cagcaggtcg
     5161 cggatattgc tgattttttt gtcgaacagc aatacaaacg gattgtccaa gccggcgatt
     5221 tgtttttccg catcgttgat gaagtaagga gacaggtagc cgcggtcgaa ttgcatacct
     5281 tcaaccacgt ccagctcgtt ttccaaagat ttgccgtctt caacggtaat cacgccttct
     5341 ttgccgactt tttccatcgc ttcggcaata atcgcgccga cttgttcgtc agagttggcg
     5401 gaaatagagc cgacttgggc gatttcttta gaagtgtcgc aaggtttggc gatgtttttc
     5461 agctcttcaa ccaaagcggc gacggctttg tcgataccgc gtttcaggtc ggtcgggttc
     5521 ataccggcgg taacgtattt cataccttcg gcaacgatgg attgcgccaa tacggtggcg
     5581 gtagtcgtac cgtcgcccgc cacgtcgttg gttttggacg cgacttcttt caccatttgc
     5641 gcgcccatat tttcaaactt gtctttcagt tcgatttctt tggcgacggt tacgccgtct
     5701 ttggtgatgt gcgggccgcc gaaagcgcgg tcaaccacca cgttgcggcc tttgggaccc
     5761 aaggttacgc gcacggcgtt tgccaaaatg ttcacgccgt ttaccatttt ttggcggact
     5821 tcattgccga attgtacgtc ttttgctgcc atttcaattc tccaaaaatc attaaaactg
     5881 tctgataaaa ccgtttatgc cgtctgaagg cggtttgccg tttcagacgg catcgtgtcc
     5941 gtatttattt ttcaacgatg ccgaaaatat cttcttcgcg cattaccaac agctcttcgc
     6001 cgtcggcttt tacggtttgg ccgctgtatt tgccgaagat gattttgtcg ccgactttga
     6061 catccagcgg acggcgcgcg ccgtctttac cgattttgcc cgcgcccacg gcgatgactt
     6121 cgcccatgtc gggtttttcg gcggccgcac ccggcaaaac gatgcccgat gcggtttttt
     6181 cttcagcttc caagcgtttg acgacaacgc ggtcgtgtaa aggacggatg gtcatattta
     6241 tgctccgata aaatgatttg aaaacaatca tctgcccgaa cggttcggac agattgaagt
     6301 ggaaaccggg ctgccgcctg ccggcccgcc cgtataagtc ggcaaaatta gggtgtgtgc
     6361 gggtaaattc aagtgaggcg gaaaaaaatt tatttccgcc gttttttata gtggactaaa
     6421 tttaaggggc tgtactagat tagcagatat gttaccctcg aaatatgaag ataacgcact
     6481 gcaaattaaa gaaaaaagta cagaaagaac tgctccattt ttttgtactg gaagttaccg
     6541 cccgttctgc caccgatatt ttgggtatcc atcccaattc ggcagcactg ttctaccgta
     6601 aaatccgcac ggttatcaac catcatttag ccttggctgc cgatgaggtt tttgagggcc
     6661 ctgtcgagca ggacgaaagc gatttcggcg gacggcgtaa aggcagacgt ggtcgcggtg
     6721 cggcaggaaa agtggttgtc ttcggcattc tgaaacgcaa cggacgggtc tataccgttg
     6781 tcgtaaacaa tgccaagtct gaaacgttac tccctgtcat caaaaagaaa atcatgccgg
     6841 acagtattgt ttataccgat agtctgagca gctgcgacaa gttggacgtg agcggtttta
     6901 tccattaccg catcaaccat tccaaggagt ttgcagaccg tcagaaccac attaacggca
     6961 ttgagaactt ttggaatcag gcaaaacgcg tcttgcgaaa atacaatgga atcgatcgca
     7021 aatctttccc gctgttcttg aaagaatgcg aatttcgatt taacttcggc acaccgtccc
     7081 ggcagctaaa aatcctacag gattggtgtg ggatttaggg ctaatctagt acagccccta
     7141 aaatttttcg ttttcaaacc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
     7201 cacatagatt gtaaacaatc ggtcacaagc cggtttgttt tttcagaaga cattatccct
     7261 gtcagacgct gtttctatat attttgccta taacggcttg tttttaataa ataattcaag
     7321 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
     7381 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
     7441 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
     7501 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
     7561 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
     7621 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
     7681 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
     7741 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
     7801 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
     7861 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
     7921 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
     7981 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
     8041 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
     8101 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     8161 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     8221 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     8281 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtattcgccg gcgttacgtc
     8341 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     8401 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     8461 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     8521 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     8581 acgcaagcac ctgaacgctt tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     8641 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     8701 gaaaaattac gaaggttatc cggatggttt cctcagtatt ttcggctggg ggatgtcggc
     8761 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     8821 caacgtcaaa gacgaacacg aacatgaaca aggagaagaa aaatgagtac ttccgccatt
     8881 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     8941 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct tcagacggca
     9001 ttttgatgtt cggcttgcag gcaggcgagt tcgtttgcca tttgctgttc taaggtttcg
     9061 cgtcggcgga tgagtttgta tccgccaccg ttaaccaaca cttccgccgc acggttgcgc
     9121 gcgttgtaat tgctcgccat actggctccg tatgcgcccg cgctgcggat aagcagcaaa
     9181 tccccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcgcaaatc
     9241 ggaccgacga tgttggcggt cagcgtcgcg atgtctttgg tttcgaccgc ctcgatgtga
     9301 tgataggcat cataaagcgc cgggcgcatc aaatcgttca tcgccgcatc gaccatcaca
     9361 aagtttttct cttcgccgta tttgacaaac tcgacgcgtg tcagcagcga acctgcgttg
     9421 ccgaccaggc tgcggccggg ctcaagaatg agtttcagac ggcgtgtgcc gatcagtttt
     9481 tgaacggctt gggcatacgc gcccaaatca ggcacatctt cgtcttggta aacaatgccg
     9541 acgccgccgc ccaagtccaa gtgttccaac acaatgcctt cggcggcaag cgcgtcaacc
     9601 aaaatcaaaa tgcgctcgca ggcttcgacc agcgggctta agtcggtcag ttgcgaaccg
     9661 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcgtg gcggtaggct
     9721 tcgagcgcgt cggcgtaggc gatgccgaat ttgttggctt tcagacctgt ggagatgtag
     9781 ggatgggttt tcgcatcgac atcgggattg acgcgcaggg agacgggcgc ggttttaccc
     9841 aaacgcgcgg caactttctg aatacggtcg atttcgggga tgctttccat attgaagcat
     9901 ttcacgcctg cattcagcgc gaactcgatt tccgcctcgc ttttgcctac gcctgaaaat
     9961 atggtttttg ccgcgtcgcc gcctgccgcc aaaacgcgtg ccaattcgcc gccggacaca
    10021 atgtcaaaac cgctccccaa agaagcaaag tgtttgatga tgctcaggtt gccattggct
    10081 ttaaccgcat agcagacgag cggggagagc tcggcaaagg cagtttggta gtgttcaaat
    10141 gcttcggtca gcgcggattg gctgtacaca taaagcggtg tgccgaatgc ttcggcaagg
    10201 cgggggtagg ggacttgttc gcaaaatagg gtcatatttt cgttttcatt tttgggttgg
    10261 aggggcggat tgcggtttgc cttgaagctg caaaccggtt tggattacgc cgaaacgcgc
    10321 cttgtcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggccg agagcaggag
    10381 ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt cataagcgcg
    10441 aatgtggcaa gattcggcat cttaaacaaa aaacacgcaa aaagctatga tgaccgaaag
    10501 cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa tcgacgaaaa
    10561 cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag ccggagacgg
    10621 cacgcaaatc atcgtcaacc gccacacgcc caatcaggaa ttgtgggttg ccgccaaaag
    10681 cggcggctac catttcgccg agcaaaacgg caaatggctg gcaacgcgcg acaaccgcga
    10741 tttttatgac gttttaaacg aagccctgag cgcggcttcg ggcgaagcgg taaagattgc
    10801 cgaattgtaa tttgagtgtt atcacggaaa gaaaaaatga acacacgtcc cttttatttc
    10861 ggacttatat ttatcgcgat tatcgctata cttgctaact atttaggaaa cactgatttt
    10921 tcccatcatt atcatatcag tgctttaatt attgctatct tgctgggaat ggcaatcggc
    10981 aataccattt atccgcaatt ttcaacacaa gtggaaaaag gcgttttgtt tgccaaaggc
    11041 gtgcttcttc gcactggcat tgtgctgtat ggtttccgcc tcacttttgg cgatattgcc
    11101 gatgtaggat taaatgctgt cgttaccgat gcaatcatgc taatttcaac cttctttttt
    11161 accgcacttt taggcattcg ttatctaaaa atggataaac aattggttta tctcactggg
    11221 gcaggttgca gcatttgcgg tgcggcagca gtgatggcgg cagagcctgt taccaaagca
    11281 gaatcccata aagtttcagt ggcgattgcc gtagtggtca tttttgggac gcttgctatt
    11341 tttacttacc ccttgttcta cacgtggtca caagatttaa ttaacgccca tcaattcggt
    11401 atttatgttg gttctagtgt acacgaagtg gctcaagttt atgcgattgg ggaaaatatt
    11461 gatcctatcg tggcgaatac tgccgtcatt tccaaaatga tccgagtgat gatgctcgcc
    11521 ccctttttat taatgctttc ttggttatta acacgtagtg atggggtatc agaaaatacc
    11581 tcacacaaaa ttacaattct ttggtttgct gtacttttta ttggtgttgc catttttaat
    11641 tcttttgatt tattaccaaa agaactcgtg aaattattcg ttgaaatcga ttctttctta
    11701 ttaatttcat caatggctgc gcttggctta acgacgcacg caagcgcaat caaaaaggca
    11761 ggattaaaac cgtttgtttt aggaatacta acttatttat ggctagtggt tggtggattt
    11821 ttagtgaact atggaatatc aaaattaata taaaattcac taaagagagc gttacccaat
    11881 ggcacaatta ccgctatatc tgactgccga aatcggaaat tttactgtcg gcacgcctaa
    11941 agttttagaa tcattttcca aacatatccc ttatggtgtc gtctttgaag acgacggcga
    12001 cacgggctac ttctatgctg cttcgcaaga cgggatttta gatgccttgc acatctacaa
    12061 tgtcgaagat gtatccgaca aacatatccc caatcatgtc ttgattttat gggatgatgc
    12121 ctgcaccata gccgcattgt gtgtcaacga ctacattcat gccgtctatg attttgtcga
    12181 acaggcagga tattgccgca acggcttccc tgaagcaggc ggcgaatggg tgaaagtcga
    12241 aaaccgcgtc ttggatgatg aattgctgga caaaatccta tcccgagaac ctacataacc
    12301 cgcacaaaag gatacccaaa tgcccctact agacagtttc aaagtcgatc acacccgtat
    12361 gcatgccccc gccgtacgcg tggcgaaaac catgaccacg cccaaaggcg acaccattac
    12421 cgtgttcgac ctgcgctttt gcattcccaa caaagaaatc ctgcctgaaa aaggcataca
    12481 cacgctggag catttgttcg caggttttat gcgcgaccac ttgaacggca acggcgtgga
    12541 aatcatcgac atttccccga tgggctgccg caccggtttt tatatgagcc ttatcggcac
    12601 gccttccgaa cagcaggtcg ccgatgcgtg gctggcttcg atgcaggatg ttttgaatgt
    12661 caaagaccaa agcaaaatcc ccgagttgaa cgaataccaa tgcggcactt atcaaatgca
    12721 ctcgctcgcc gaagcgcagc aaatcgcgca aaacgtgttg gcgcgcaaag tggcggtgaa
    12781 caaaaacgaa gagctgacgc tggatgaagg gctgctgaac gcctaatccg ccaaaaatgc
    12841 cgtctgaaca agggtttcag acggcatttg ccttttccgt tataatctag ggttgtgcgg
    12901 gggcgggttt taagccggca tcgtccttcc ctattttttt ctgtccctta tcggttttaa
    12961 gcgggttttt tatgtccaac agacctacac tcctcctcgt tgacggctct tcctatctct
    13021 accgcgcgta tcacgcgatg gggcaaaacc tgaccgcacc cgacggtgca ccgacgggcg
    13081 cgctgtatgg cgtattgaat atgttgcgcc gtttgcggtc ggaatatccg cacgattatt
    13141 gcgcggtggt ttttgatgcg aaaggcaaaa atttccgcca tcaaatgttt gaagaataca
    13201 aggcgacgcg cccgccgatg cccgacgatt tgcgcccgca ggcggaagca ctgccggatt
    13261 tagtgcgcct gacaggctgg ccggtattgg tgattgggca ggtggaggcg gacgatgtga
    13321 tcggcacgct ggcgaaacag ggggcggaac atggtttgcg agtcattgtt tcgaccggcg
    13381 ataaggacat ggcgcagttg gtggatgagc gcgttacgct ggtgaacacg atgagcagcg
    13441 aaacgctgga cattgaaggc gtgaaggcaa aattcggcgt gcgccccgac caaatccgcg
    13501 attatctcgc gctgatgggc gacaaggtgg acaacgtgcc gggcgtggaa aaatgcggcc
    13561 cgaaaacggc ggtgaaatgg ctggaagcct acggttcgct ggctggtgtg atggaacacg
    13621 cttcggaaat caagggcaaa gtgggcgaaa acctgcaagc cgcgctgccc caactgccgc
    13681 tgtcatatga tttggtcacg attaaaaccg atgtggactt gcacgccgag ctttcagacg
    13741 gcatcgaaag cctgcgccgt actacgccga aatgggcgca gctggttgtc gatttcaaac
    13801 gctggggctt ccgcacctgg ctgaaagaag cggaatcaaa catgaatacc ggctcgaccg
    13861 atgatttgtt cggcagcgac agcatcggcg agcaggcggc tttgaatgcg gaaatgccgt
    13921 ttgaaaaaca agccgaaaaa gccaccgccc ccgaaaaact ggattatcaa gccgttacca
    13981 ccgaagcgca gtttgccgct ttgttggaca aactgtcgcg ggcggacaca atcggcatcg
    14041 atacggaaac cacgtcatta gacgcgatga acgcctcgct ggtcggcatc agcatcgctt
    14101 tccaagcagg cgaagcggtt tacatccccg taggacacag cctgaccgcc gcgcccgaac
    14161 agcttgattt gcaagacgta ttaggccgtc tgaaaccgca tttggaaaac cccgccctga
    14221 agaaaatcgg gcaaaacctc aaatacgacc aacacgtttt cgccaactac ggcatcgccc
    14281 tgaacggcat tgccggcgac gctatgctcg cttcctacat catcgaaagc catcttggac
    14341 acggcttgga cgaattgtcc gaacgttggc tgggcttgga aaccattacc tacgaatcat
    14401 tgtgcggtaa aggcgcgaag caaatcagtt ttgccgatgt cgccatcggg caggcgaccg
    14461 aatacgccgc ccaagacgcc gatttcgcct tgcgcctcga agcgcacctg cgcgcgcaaa
    14521 tggacgccaa acagcttgaa atgtatgaaa aaatggagct gcccgtcgcg caagtattgt
    14581 ttgaaatgga acgcaacggt gtgcaaatcg accgcgccga actcgcccgc caaagcgcgg
    14641 aactcggcgc cgagctgatg aagctcgaac aagaagccta cgccgccgca gggcagccgt
    14701 tcaacctcaa ctcgcccaaa cagctgcaag aaatcctgtt cgacaaaatg ggcatcccga
    14761 caaaagggct gaaaaaaacc gccaagggcg gcatttccac caacgaggcc gtgctcgaac
    14821 agctcgcgcc cgactacccc ctgcctaaaa tcatcctgca aaaccgcagc ctggcgaagc
    14881 tcaaatccac ctacaccgac aaactacccg aaatgatttc ccccaaggac ggccgcgtgc
    14941 ataccaccta cgcccaagcc gtcgccatta ccggccgcct cgccagcaac aaccccaacc
    15001 tgcaaaacat ccccatccgc accgccgaag gccgccgcgt gcgccgcgcc tttaccgcac
    15061 cgcaaggcag cgtcatcgtt tccgccgact attcccaaat cgagctgcgc attatggcgc
    15121 acctctccgg cgacaaaacc ctgattgccg cgttccaaaa cggcgaagac gtacaccgcc
    15181 gcaccgccgc cgaagtgttc ggcactgcgc ccgaaaacgt ctcgcctgag caacgccgct
    15241 acgccaaaac catcaacttc ggcttaatct acggtatggg gcaatacggc cttgccaaat
    15301 cattgggcat cgacaacctt tccgccaaaa actttatcga ccgctacttc gcccgctacc
    15361 ccggcgtcgc cgaatacatg cagcgcacca aagaacaagc cgccgcccaa ggcttcgtcg
    15421 aaaccctgtt tggccgccgc ctctacctgc ccgacatccg caacaaaaac gccaacgccc
    15481 gcgccggagc cgaacgcgcc gccatcaacg cccccatgca aggcaccgcc tccgacctta
    15541 tcaaacgcgc catgatagac gtgcgaaact gtctttcaga cggcatcggc agcaaactgg
    15601 tcatgcaggt gcatgacgaa ctggtgctgg aagtcgttga aaccgaactg gattttatca
    15661 aagaaaaact gccgcagatt atggcgaaag tggacggcgg attattggat gtaccgctgg
    15721 tggctgaggt tggcgtaggg gagaattggg aagaggcaca ttgattgaaa ggtgttatat
    15781 gctatcttta tttaaataaa atttaatttt tggtatattt tttctaaatg ttccta
//
LOCUS       DAXHTY010000043        15584 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXHTY010000043 DAXHTY010000000
VERSION     DAXHTY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.043"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(1..1185)
                     /locus_tag="ACFNMK_001125"
                     /pseudo
     CDS             complement(1..1185)
                     /locus_tag="ACFNMK_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1331..2035
                     /locus_tag="ACFNMK_001126"
     CDS             1331..2035
                     /locus_tag="ACFNMK_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC6364408.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(2099..2665)
                     /gene="dcd"
                     /locus_tag="ACFNMK_001127"
     CDS             complement(2099..2665)
                     /gene="dcd"
                     /locus_tag="ACFNMK_001127"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC6364409.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2731..3147)
                     /locus_tag="ACFNMK_001128"
     CDS             complement(2731..3147)
                     /locus_tag="ACFNMK_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFC6364410.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(3199..4098)
                     /locus_tag="ACFNMK_001129"
     CDS             complement(3199..4098)
                     /locus_tag="ACFNMK_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC6364411.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(4129..5586)
                     /gene="der"
                     /locus_tag="ACFNMK_001130"
     CDS             complement(4129..5586)
                     /gene="der"
                     /locus_tag="ACFNMK_001130"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC6364412.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEEIL
                     ENFPEPEVEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENADDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5736..6365)
                     /locus_tag="ACFNMK_001131"
     CDS             complement(5736..6365)
                     /locus_tag="ACFNMK_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC6364413.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6366..7661)
                     /gene="hisS"
                     /locus_tag="ACFNMK_001132"
     CDS             complement(6366..7661)
                     /gene="hisS"
                     /locus_tag="ACFNMK_001132"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC6364414.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            complement(7755..8411)
                     /locus_tag="ACFNMK_001133"
     CDS             complement(7755..8411)
                     /locus_tag="ACFNMK_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6364415.1"
                     /translation="MKQKRRFTASLLLAAALPLYAHSFPFAEENPIAYGKVKIQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLKKLDKEQMRASFEDMQRIMP
                     DLGFEAKGYALSFEQLAQLKIPIIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLDAWKTREGNLAGKILAVVPKKAETISNKLFFTHHPKRQTEFAVEQIRQARAE
                     "
     gene            complement(8571..8858)
                     /locus_tag="ACFNMK_001134"
     CDS             complement(8571..8858)
                     /locus_tag="ACFNMK_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364416.1"
                     /translation="MDDLIAAFLGYLSGKYSEGKISRLEISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWNFITEMIKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
     gene            complement(9061..9276)
                     /locus_tag="ACFNMK_001135"
     CDS             complement(9061..9276)
                     /locus_tag="ACFNMK_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364417.1"
                     /translation="MVNKYIKTAISITAISIFYLLTQESSGKTEEPSYFLMFMFLNSL
                     WFEENKTVMAAVTAMIAAHLIFVTLSD"
     gene            complement(9266..9661)
                     /locus_tag="ACFNMK_001136"
     CDS             complement(9266..9661)
                     /locus_tag="ACFNMK_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364418.1"
                     /translation="MKKQITAAVMMLSMIAPSMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAWGPVGAFIGGVGGGIGYMMNQQLSGSRFSPTAFTLAVGAGAFGGA
                     TAGPAGVVWGFNRSLATGMVTGVAQRYGK"
     gene            complement(9704..10573)
                     /locus_tag="ACFNMK_001137"
     CDS             complement(9704..10573)
                     /locus_tag="ACFNMK_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC6364419.1"
                     /translation="MKRIFLSALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENSRAALAAPVYIQTGATSFIPIPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGKQPDRIDGKKESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKLGVQHTF"
     gene            10739..11662
                     /locus_tag="ACFNMK_001138"
     CDS             10739..11662
                     /locus_tag="ACFNMK_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6364420.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLLYPLDVVSRLVRGEKFFTTQEAEGV
                     PNAHTTVELIENRGGFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(11740..12381)
                     /locus_tag="ACFNMK_001139"
     CDS             complement(11740..12381)
                     /locus_tag="ACFNMK_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6364421.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVSARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGNSEGTHDYGHGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(12436..13227)
                     /locus_tag="ACFNMK_001140"
     CDS             complement(12436..13227)
                     /locus_tag="ACFNMK_001140"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC6364422.1"
                     /translation="MARHPYRRLRPAKSSFPEVGISEEGNIRSLHLGSDTIQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARSLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLADLKASNPNNGKHFYL"
     gene            13393..13468
                     /locus_tag="ACFNMK_001141"
     tRNA            13393..13468
                     /locus_tag="ACFNMK_001141"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13426..13428,aa:Thr,seq:tgt)
     gene            13503..13578
                     /locus_tag="ACFNMK_001142"
     tRNA            13503..13578
                     /locus_tag="ACFNMK_001142"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13536..13538,aa:Thr,seq:tgt)
     gene            13720..14511
                     /gene="panB"
                     /locus_tag="ACFNMK_001143"
     CDS             13720..14511
                     /gene="panB"
                     /locus_tag="ACFNMK_001143"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC6364423.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            14634..15470
                     /gene="panC"
                     /locus_tag="ACFNMK_001144"
     CDS             14634..15470
                     /gene="panC"
                     /locus_tag="ACFNMK_001144"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC6364424.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAACLGTTRLIDNLEIK
                     LP"
BASE COUNT         3481 a         3628 c         4345 g         4130 t
ORIGIN      
        1 cccgtatttt cttaccgtat tctgcgattg cctgtcggaa aatgccgatc aacctgccta
       61 taacggcatt ttcgccaaat tcgttcagac agttttccct aagtcgggca ggttcaaaat
      121 cagagtggtg ttcatacatt ttgatgagtg cgtcggcaag ggcatcgtcg tcgtcaacag
      181 gaaccaaata tccgttgccg tctgaaacaa tagattccgc accgccgcag cgtgttgcaa
      241 tgacgggcaa tccttgggac agtgcttcga tatagactac gccgaaggtt tcggtgcggc
      301 tggcaaggac gaatgcgtcg ctgttcctca tcaaatccaa gactgcttcg ggctgcaatg
      361 cgcccaaaaa tgtaacggca tgggtaatgc ccaagtctgc cgcctgctgt ttcagccgct
      421 gttcttcctg tccgctgccg ccgatgttca ggcgcagttg cgggcattgt gccaacgccc
      481 gggcaaaggc agtgagtagg acatcgtgtc ctttgagacg gcgaaggtgc gagacggtgc
      541 agaacacgaa atgcggtttg ttgttttttt ctctttgttc aaacggtcgg ttgaatattc
      601 cgcccagtat gttgggggag gtactgccat tcgcagccgt atttgtgttg caggacgtgt
      661 gcgaaatggc ggctgacagc gagcagtgcg gaggcgtgcg ccgctgcttt ttccatcggc
      721 tgccattggt ggggacggat cagaccgcgc gtgatggtgc tgctgtgttc cgtgacgaca
      781 taggggatgc cgtatttttg ggaaattttt tgggcgagta tgccggcata gttcatgcgg
      841 tgggcgtgaa tcaggtcggg cagcccgttt tcgcggatgt agtgtttgaa agctttcaaa
      901 cccgcacaca cccagcggat gcagtcgatg tcgatgaacg gaaagcgggg gaagaaatat
      961 atgccgtgcc atgcatagat gtccaaaccg ctttgccgat attttgcaaa accgtaaggc
     1021 cggtcaggat gcttgctgtt tctttccgca agtagcggaa catcggtgca agcacggcgg
     1081 ttttgatgcc tttcctctgc aatgccagtg cctgattttg aaaaaaatcc cgtccacatc
     1141 ctgttcggat tgcggatacc atgaggggat gacgaggacg tgcaagggtt cgggcatagt
     1201 gggattccgt atcggaaagg cggttattat aagacagacg cagaccgaat atttaaattg
     1261 ttgccttacg ctaatgcaat ttagcgcgcc gatatgctag attggcggtt ttataagtaa
     1321 ggatacggat atgttgcgtt ctattttggc ggcttccctg ctggcggtat cttttccgac
     1381 ggcggctgaa gcattgaatt acaatattgt cgaattttct gaatcggcgg gtgtcgaggc
     1441 ggttcaggat acaatgtccg cacgtttcca agtgacggcg gaaggacggg acaaaaatgc
     1501 cgtcaatgcc gagtttgtta aaaaattcaa caatttcacc agaaaatcaa aaaatggtag
     1561 ctttaaaacc gaattggtat cgcgcagtgc gatgccgcgc tatcaatata ccaacggcag
     1621 acgcattcaa acaggttggg aggagcgtgc ggaatttaag gtcgagggta gggattttga
     1681 tgcgttgaac cgttttattg ccgatgttca ggcagatgcc gcgttggaat atacggattt
     1741 ccatgtgtcg cgcgaacgcc gcaacgaggt catcgatcag gtcagcaagg atgccgtttt
     1801 gcgtttcaag gcgcgtgccg aaaagttggc gggcgttttg ggtgcgtccg gttataaaat
     1861 cgtcaaatta aatttgggac acatcggcag ccatatcgcg ggagggggag ctgctcaggc
     1921 aaaaatgctt cgtgccatgc cgatggcggc aagcgtcaat atggagggtg cggattccgc
     1981 cgcgcctggt gtggaggaaa tcagcatcag cgtcaatggg acggttcagt tctgatttga
     2041 ggtgaacggc aaatgccgtc tgaaacccga cgataagggt tcagacggca tttatatttc
     2101 aggctttggg cagggtaacg ccggtttgcc ccatatattt gccgttgcgg tctttgtatg
     2161 aggtttcgca gatttcgtcg ctctcgaaga agaggacttg cgccacacct tcgcctgcgt
     2221 agattttggc gggcaggggg gtggtgttgg aaaattcgag ggtaacgtag ccttcccatt
     2281 ccggttcgaa cggggtaacg ttgacgataa tgccgcagcg ggcgtaggtg gatttgccca
     2341 agcagacggt caggacgttg cgcgggatgc ggaaatattc gaccgtgcgc gccagtgcga
     2401 aggaattggg cgggatgatg cagcagtcgt cttcaacggt aacgaagttt ttcgggtcga
     2461 agtttttggg atcgacgatg gtgctgttga tgttggtgaa gattttaaat tcgtttgcgc
     2521 agcggatgtc gtagccgtag ctggacgtac cgtaggagat gatgcgtttg ccgtcggctt
     2581 ctttgatttg gttcggctcg aaagggtcga tcatgccgaa ttcttcgctc attcggcgta
     2641 tccatttgtc ggacttgatg ctcataatgt tttccttgtt tcttgcagtg ttcggacaaa
     2701 gcattggggg atgccgtctg aaaacggggc ttatttgttt ttgggcagct tcacttcttt
     2761 aatcatgccg ttttcgcatt tcataatgaa gagggtttcg ccgttttgcg agacggtaaa
     2821 tctgccgtgt gccaagcctt ttttgaatgt gcccgagagt accatgttgc ggaatttggt
     2881 actgtcggaa ttgaacggtt cgataaatat ttcgcggttg gcggcaacgg tataaacgcc
     2941 ttgtccgtcg aatttgccat ttttaaacga accggtatag ttgcgcccgt cttggcagcg
     3001 ccatgtgcct ttgccggcgg gtttaccgtc tttgccgaca tcgccttcgt aggtgcagcc
     3061 gggttcttga taggaagtca gaacggcgga cgcggcgggg agggcgaaca tcatggcggg
     3121 cagtaggaat gcgagatgtt taagcataag ggttattcca ttggattttg gctgacggta
     3181 taaaagccgt ctgaaaaatc aatcttgcca gccgcccaaa taggaaacca gctcttccaa
     3241 catgatgctg acggattccg ccatcaggat ttgcgaggcg aaggcgagac cggcggcatc
     3301 gtcgccgttg ctttcggctt cttcctgcaa tacgtcgagg tattggatgc gcttgagtgt
     3361 gaagtcttga gtgaggataa aggcgatttg ttcgcgccaa accaaaccga gctgggttac
     3421 ggttttaccg tttttgacgt gttgaaccac ttcgtcggcg gttaaatctt gtttggatac
     3481 tttgacgacg ggaacaatat cgcccgtgcc tttgagttcg caatcgctgt ccaattcaaa
     3541 accgccttcg caatgccctt gcaacagcca gccggtcatc agggaagagg gcgattgttt
     3601 ggtattcggc agtgaggctt ccaaaccgcc caaagcttcg cgcagcttgg tcaggatgtt
     3661 ttctgctttg gcggaagccg cgttattgac gagcaggtag ccgtggcggg tgttaaacac
     3721 cgcttctgta cggctgctgc gggtaaacgc tcggggcagc aggtcgtctg taatttgctc
     3781 tttaagctct tgtttttctt tgcgaccgac attgcgggct tcattgtttt ggatttccgc
     3841 taccttctct tccaaaatat cgcggatgac gccggcaggc aggacttttt cctctttttt
     3901 cagggcgacg cgcaaggtaa agtcggcagg aaaaacgagt tccggggaga atgaaaccgg
     3961 tgcggtaaag ccttcgctga accagtctaa gccttggcaa tgggtaaatt cagcttcagc
     4021 aagtttgtcg gcaagtacgt ccgcctcagg cagcttttct ttgttgagcg gataaaaact
     4081 aatctgcttg aaccacataa tgtttcctgt tgtttgaaat gtcgggaatt atttgctgaa
     4141 ttgttttttc acactgactt tggttttctt cttgaagcgg tttttctctt ctttgcggcc
     4201 ttcgcgtttc tcaatacggt tgctcaggct gacgcggcgc agcggttttt tcttcggttt
     4261 gtcgtcggcg ttttcatacg ggttttctga aacattgtat tgaatccgca acggcgtgcc
     4321 ttgcaggttg aaggctttgc ggaacgtttg ggtcagatag cgcgtatagc tgtcggaaat
     4381 cgcgtgcagc gaattgccgt gtaccacaat tacgggaggg ttcatgccgc cttggtgggc
     4441 ataacgcatt ttcggacgca ccaagccggc acgcggcggt tgctgacgct cgattgcgct
     4501 ttgcaggacg cgcgtgattt tcggcgtcgg catcttaatc atcgccgcgt tgtaggcagc
     4561 ctgaatgctg tcaaacaaac cgtctatacc gcgctctttc aatgcggaaa taaagtggaa
     4621 cttggcaaaa tcgaggaaat acagtttgcg gttgatatcg cgtttcactt gctcgcgacg
     4681 ttcttcgctg atgccgtccc atttattgac cgccaccacc aaagcacggc ctgcctccaa
     4741 agcgaaacct gcaatcgtcg catcttggtc ggcgatgtcc tgctgcgcgt ccaataccaa
     4801 aacagcgacg tttgccgctt caaccgcctg catcgctttg ataacggaga atttttccac
     4861 tgcttcatcc actttgccgc gacggcgcac acctgcggta tcgatgatgg taaacggttt
     4921 gccttcgcgc tcgaaatcga tatggatact gtcgcgcgtc gtgccggcca tatcgaaggc
     4981 aatcacgcgc tcttcgccga gaatggcgtt taccagcgtg gatttgccga cgttcggacg
     5041 accgatgacg gcaaaaacgg gatgttttgc atcggcttct tcgacttccg gctcggggaa
     5101 gttttctaaa atttcttcaa tcaggtaata cacgccgtcg ccgtgcgcac cggaaataac
     5161 ataagggtcg cccaaagcaa gttcgtagaa ctcggcggca agcacggccc tgttgccgcc
     5221 ttcgcctttg tttaccgcca gataaacagg gcgcgggctt tggcgcaaac ggtcggcaat
     5281 gattttgtct tgcggggtca gaccggtgcg gccgtccacc aaaaatacga cggcatcggc
     5341 ttcatcgaca gcctgcaagg tttgttttgc catttcgtgc aaaatgccgc tgtccacaac
     5401 cggctcgaaa cctccggtat cgatgaccaa ataaggtttg ctgccgactt tgccgtgtcc
     5461 gtaatggcgg tcgcgcgtca gaccgggcag gtcgtgtacg agcgcgtctt tggtgcgcgt
     5521 caaacggttg aataaagtag atttgccgac gttggggcgg ccgacaagcg cgatggttgg
     5581 tttcatgatt gggtctttct gtgtcaagtg ccgttcggga gaactgaaca caagcaggtg
     5641 tccgttggga cacggcagat ggttttacgg gaattgccgt aggatagtgt tgtctgaaat
     5701 gccgtctgaa gagagggtgg catttcagac ggcatttatt tcagcgaatc gagtttcatt
     5761 tgaaccaatt cacgaccgac agaatcttga ggcatttttt ctaaagcctg accgtagttt
     5821 tttaaggctt cctggctttt tccctgtgcg gcatagacat cgcctttggt ttccatcagc
     5881 aggggggaga agtccgcttc aaccggcgtg tcgagcgcgg caagcgcggc atcgtatttt
     5941 ttttgttgca acaacacaac gcccagacgc tgcgccgcca acgcttgaat caggctgtct
     6001 ttttggttgg acaacaccca tttcaaatgg ccttcggcaa catcgtaacg ctgcgcgtca
     6061 aattcggttg ccgccgccat cagcgtggct tgggcggcgg aaatagaatg cgggtagctt
     6121 tgttggagtt tggtcaattc ggcattaatt tcgctttgcg gggctttgtt ttgcgccttt
     6181 tccacgatgt ttgccagcac cgccgccgct tcctgatttt gggaagccgc acggttttgg
     6241 taaaccgtgt atcccaagta gccgagtgct gccaaaatca gcagggcaaa cagccatttg
     6301 cccgtggttt tccaaaaata tttaaagttg tctaactctt gttgttcttc gagatgggct
     6361 gccatttatg cgttcttcca ttgttgtaaa gtatgggtta aatcctcggc ggcgacagtt
     6421 tgctgaccgt gtgcgccgtt catgtctttg agcgtaaccg taccgttcgc cagttcgtct
     6481 tgcgcgacaa tcagggcaaa gcgcgcgccg ctgttgtcgg cttttttcat ttgcgctttc
     6541 aggctttgat agccggaatg ctgcattaca ttgaaacctt gcgcgcgcaa ggcttgcgcg
     6601 tatttcatca cctgcaagtc cgccctttcg ccttggtgca ttgcatagac atcaggcgca
     6661 gcgttcactt ccagcgagcc gtattcgctc accaaaagca gcagccgttc gatgcccatc
     6721 gcaaagccga tggacggcgc aggtttgccg ccaagttcct caatcaaacc atcgtaacgg
     6781 ccgccgccgc acacagttgc ctgcgcgccg agtttgtcgg tcgtccactc aaaaaccgtc
     6841 tgattgtaat aatccaaacc gcgaaccaag cgcggatttt caatatattg gatacccaaa
     6901 ccgtccaaca tcgccttgaa gcgtgcatag tggttttgcg aagcctcgcc caaataatcc
     6961 accaaacgcg gcgcggcgtt gcagatttcc tgcaaatcag ggtttttcgt atctaaaaca
     7021 cgcaaaggat tggttttcag acggcgtttg ctgtcttcgt ccaatttatc ttcatagcgt
     7081 gtcaaatatt caaccaacgc cgcacggtgt gccgctcgct cctcacggtt gcctaagctg
     7141 ttgatttcca aagtcaggta ttcgcggata cccaattttt cccataagtc ggcagacatc
     7201 gcgatgattt ccgcatcaat atccggacct tcaaaaccca aagcttcgat accgacctga
     7261 tggaactgac gataacggcc tttttgcgga cgctcgcggc ggaacatcgg ccccatatac
     7321 cacagctttt gcgggctatt gtacagaagg ttgtgttcga ccaccgcacg cagacaagaa
     7381 gccgtacctt caggacgcag gctcaaactc aaagaatcgt ttgaatcgga gaaggtgtac
     7441 atttccttgc cgaccacatc ggtttcttcg ccgatggagc ggacaaacaa accggtttgc
     7501 tcgacaatcg gcgtacggat ttgctgataa ccgtaagcgc gtgtccagcg gccgaccgta
     7561 tcttcaaacg cctgccaaaa cgcagccgtc agtttgaaat ctttttgctc gacaggcaga
     7621 aggtcgttca tgcctttgac ggattggatt ttttgtgcca tttcaagtaa gaatgcttaa
     7681 atcaaattgc gggcgattat agcggatttt taagggcttg tgaggttggg ggtggtttgc
     7741 ggatggcatt tgacttactc tgcacgtgct tgcctgattt gttcgactgc aaactccgtc
     7801 tgccgcttgg gatgatgtgt gaaaaacaat ttatttgaaa ttgtctcggc ttttttcggt
     7861 acgacagcca aaatcttacc tgccaaattt ccctcacggg ttttccaagc atccaaaaac
     7921 tgcgccctgc tcatggaaac atgccccagc gacggatcgg caagcaaaac cgtattgccg
     7981 tctatgccgc gcaacaccga gaaatgatca tccttgcggt atttcagata cacgatgatg
     8041 gggattttca actgtgcaag ctgctcgaaa gacagagcat agcctttcgc ttcaaaaccc
     8101 aaatcaggca taatgcgctg catatcctca aacgacgcac gcatctgctc cttatccagc
     8161 tttttcaaca cttcttcttc cgtcagcgtt tgcccgtaaa aattattcaa aagcgtcgcc
     8221 accgaagccg ccccgcagga aaaatccaaa tcctgcttta caatattgaa atcccgccgc
     8281 gctttccaac tctgtatttt gactttgccg taggctatgg ggttttcttc cgcaaaggga
     8341 aaagaatgcg cataaagagg tagggcggcg gcgaggagca gggaggcggt aaaccgcctt
     8401 ttttgtttca tcaaaatcct cctgaaaggc tacccggaat ttttaagcag ccttttgatt
     8461 ggtaaacgtt acgacggaag ccttagcctc ccgattgcag tatttcaaat ccaagataaa
     8521 tgccgcatag aaaataaaat acttaaatgg tttttagcgg cttaaatatc ttatttaatt
     8581 ttattgattg tttttccaat agtttctgca ataaataata agaagtagaa taacaaacaa
     8641 acagaacatg aaaataaaaa taaatctttt atcatctcgg taataaaatt ccaaagattt
     8701 tccttatttc catcaacaat aaaaattatc agttcatgaa taaagaatac tgcaaagaaa
     8761 atgcttcctg ccgttaggga tatttctaac ctgcttattt ttccttcgct atatttacct
     8821 gataaatagc ccaaaaaagc tgcaatcaaa tcatccattt taaaaatttc tgtaaaaaca
     8881 gataatgtcc ttaattcatt ttgtccaatc cgtttgccat tgcggggcaa tcatagacag
     8941 catcatcacg gctgcggtga tttgtttttt cataacaaat tcttagggag gagataatgt
     9001 ttcatcaaag tcctcctgaa aggctgcctg aaatttccaa gcagcctttt tgtttgctta
     9061 ttaatcggat agtgtgacaa atataaggtg ggcggcaatc attgccgtaa cggcagccat
     9121 aactgtttta ttttcttcaa accaaagcga gttgagaaac atgaacatca agaaatatga
     9181 tggttcttca gttttgccgc tgctttcttg agttagaaga taaaatatgg atatggcggt
     9241 tatgctgatt gctgttttta tatatttatt taccatatcg ttgggcaaca cccgtaacca
     9301 tacctgttgc caaagatcgg ttaaatcccc atacaacccc tgccggacct gctgtcgccc
     9361 ctccaaaagc tcccgcccct acggcaagag tgaatgctgt gggagaaaat ctgcttccgc
     9421 taagttgctg gttcatcata tatccgattc cgcctccgac tcctccgata aatgcaccta
     9481 ccggtcccca agccccctca gtctccttca tctccttttg agaaagctcc gccaactgca
     9541 tcggcgcatc tgcctgcgtg tggaacactt ggttttcaaa tgtctgattg tccaagccgt
     9601 ttgccattga gggggcaatc atagacagca tcattacggc tgcggtaatt tgttttttca
     9661 taacaactcc tttggattac aacgttggaa aatcaaaacc tgcttaaaat gtatgctgta
     9721 cgcccaattt cagttcggaa ctgctttgcc ctgaaacgtt gaaacgtgcg gatgcgttta
     9781 aagccgtggt tttggtgaaa ccgaaacctg cgccgaaatg ggcgtaggtg gatgtgtttc
     9841 ttgcggattc ttttttgccg tctatgcggt cgggctgttt gcccagccat tggatgcctc
     9901 cggtcaggct gattctgtcg ttggcggcaa atgagatgtt ggggttgagc agcaggtagt
     9961 tgcccgattt gtagcggatg ccgtctgaaa gggttttgct gccgttgatg cggtaggcgg
    10021 cggtgaggga aaggacaatc ggatctatgg ctttgtaggt ggtggcgccg atgagccacg
    10081 attttcccga cgaggctttg ttgcgcgatt tttcgtaaac cgtgctttca agaaagctga
    10141 ttagggcggg gtttttgtcg tctttaagga aggtgtggct gatgccgagg gatacgtcgg
    10201 acatccgttt gttgcgggtt ttgccgttgc cgtcgagttt gcgttcttcg tgccacagat
    10261 agctgccgct gccgtaaatg tcggtattgc cggtcagtcc gtagcgcaaa ccgagcgtgc
    10321 cggcgagcat atcggtattg ctgccgtttt cttggatttc ggtcggaatg gggataaacg
    10381 aggttgcgcc ggtttgaatg taaaccggtg cggcaagtgc ggcgcggctg ttttcgctat
    10441 tcaggtaggt aagggaagtt tccagtttcc attttccctt gtcggtcatt atgtcttcaa
    10501 tcgtcaaggg caggtcggca taagtggata aaggcaggat ggcgggcaag gcggacaaaa
    10561 agatacgttt catatttcct cttattgata ttaattctta tttaagaata gtaatgaata
    10621 tccgataatt ctgtctaaat aaatttttaa atcaaaaaaa aacgatatat ttgtttttat
    10681 caattatgtt ttgatatacc gtctgaaaag tttgtgaaaa acggttacaa tccgccgcat
    10741 gaaaaaacgc aataatcctc ttccgctgtt gaacggtgtc aaacccagtt atttggtgct
    10801 gccgcatgaa aagcagtttt acgggctgcc gctgctgcat tttctgtgca tccgctttcc
    10861 ttttgtgggc gcggacgatt ggcgcaggcg gttgaacagc ggttttgtgg tcggttcgga
    10921 tggtgcggcg ttggacgaac attctttgtt cgagccgggt aaggtgatgt tttattaccg
    10981 tgaaaccagc cgtgagagcg agccgcgtat tccgtttgaa gaaaagattt tgcatattga
    11041 tgagcatttg attgtggtgg acaaaccgca ttttctgccc gtcatcccca gcggcaggtt
    11101 tttgcgggaa accctgctca cgcgcctgcg tttgcggcct gaattgcagc atttgaatgt
    11161 tgaggacatt acgccgctgc accgcttgga caaggatacg gcaggcgtga tgctgctgtc
    11221 gcataatcct gccacgcgcg gagcctatca gacgatgttt caaaacaaaa cggtatggaa
    11281 aacgtatgag gcgcttgcgc cgacaaggac ggatttgctg tatccgctcg atgtggtttc
    11341 gcgtttggta aggggtgaaa aattttttac gacgcaagag gcggaaggcg taccgaatgc
    11401 gcacacgacg gtcgaactga ttgaaaacag ggggggattc agcctttacc gccttacgcc
    11461 gcataccggc aagaaacacc agttgcgcgt acacatgatg ggcttgggta tgccgttgtt
    11521 gaatgacgcg ctctatcccg tgccgtctga agcgggaagc gaggattatc ggaaaccttt
    11581 gaagctattg gcaaaaaaga ttgcgtttgc agatcctttg tcgggtcggg aaagggtgtt
    11641 ttgcagcgga ttttgcctat aatgagaggg aacgcaaacc cgtatcggcg gatgccgccg
    11701 atacgggttt tacattatcg atgccgtctg aaaggggttt tatccgttca ggcatacggg
    11761 cgcgaagcgg aggatggtgt cgcgcaggac gatgagtttg ccgtgaaaga aatgcgtgga
    11821 accggcgatg gtaatgacgg gcaaatcttg cggttccgcc catttcagtg ctttcccgat
    11881 ttcgacgact tcgtcttccg cgccgtgtat catcagcgtt ttggcaacat tgggaacggc
    11941 ggacggctcg gggcggccgg tatagtggca gactgccgcg ccgatgagca gtaacaggtc
    12001 gggtatgcgc gcttgtgcgg caaatgtggc gacatagccg ccgaaggaga agccggataa
    12061 ggcaaattcg ggggcttcgg gatgttgggc gcgggcatag tcgatgacgg cgaggcagtc
    12121 ttgcgtttcg ccgtgtccgt aatcgtgtgt gccttcgcta ttgcctacgc cgcgaaggtt
    12181 gggcaggtag cagtggaagc cgagtttgct taaggctttg gcggcagttt ggatgacttt
    12241 gttggtgttt gtcccgcctt gtagggggtt gggatgattg atgacggcaa caccgcgtgc
    12301 cgatacttgt tcggacggga tatggatggt ttctaaaatg ccggcaggac cggatatgtg
    12361 tatggtttcg ggtttcagca taatgatttc ctcttgattt tccgcatgtt ccaatgcggt
    12421 catgaagata gttgcttaaa ggtagaaatg cttgccgttg ttggggttgg atgctttcag
    12481 gtcggcgagc atgcggtgga agtccaatcc gtatgcgttg ctcagtttgt cggcacgttt
    12541 tttgagtttg tctatgtttt gctctttggg gctgctttgg aaggccatta ccgcgacatt
    12601 gccgtggctt tcggcaggaa gttccaagac gcgcccttca aaaacgctca acaaccgttc
    12661 gatgaagcgt tggtagcgtt tgtcgccgct ccaccagttg gttacgaata tgccgtctga
    12721 agagagtgcg tttcggcagt ctcggaaaaa cggttcttca accagcgcat cgatgatttg
    12781 ttcgccgtcg aacccgtcca ccaaaatcac atcggtgttg tggcggaaga ctttgatata
    12841 ttctgcaccg tctgcttcga taatttcaaa tttctcgccc tcgaaaggca actcgaacag
    12901 gctgcgggca atggcgatga cctgcgggtt gatgtccacg gcggtttggc gcgtgtcggg
    12961 caggtaggtg tctatccagc gtgcaaacga accgccgccc aagccgattt gggtgatatg
    13021 ctgcggaaga gcgtcggtaa acagcagcca tcccatcatg gcacggctgt aagagagtac
    13081 cagctcggac gggtggtcga ggttcattga gctttggatg gtgtcgctgc ccaagtgaag
    13141 cgaacggata ttgccttctt cggaaatgcc gacttcggga aagctggatt ttgcaggacg
    13201 caggcggcgg tagggatgtc ttgccatcgg tgtgaacggt cggtttgaaa ggcagatatt
    13261 ttatcagaat gccgatggtt gtcctgcttc aaattaattt cttttcaaat aaattactta
    13321 ttcggatttg ccggggcttt cggataaatt ccttgccaag gtgcggcatt gcctgcataa
    13381 ttcgctttct ttgccggtat agctcagttg gtagagcacc tgacttgtaa tcagggggtc
    13441 ccgagttcga ctcttggtgc cggcaccatc cttgcttatg gtgcggtaat acaatccaat
    13501 atgccggtat agctcagttg gtagagcacc tgacttgtaa tcagggggtc ccgagttcga
    13561 ctcttggtgc cggcaccaga tttgacagcc cgattgtgta aaacagtcgg gctgttttgc
    13621 atttgcggca cggtcggtgg cagggctgcg ggcatttcac tataatagcc cgtttcaaac
    13681 tgaaaaggcc gtctgaaaag ggcggggtaa caatatctga tgattactgt gaacacactg
    13741 caaaaaatga aggcggcggg cgagaaaatc gctatgctga ccgcttacga atccagtttt
    13801 gccgcgctga tggacgatgc cggcgtggat gtgctgctgg ttggggattc tttgggaatg
    13861 gcggttcaag ggcggcagtc gacgctgccg gtcagcctgc gcgatatgtg ctatcacacc
    13921 gaatgtgtgg cacgcggtgc aaaaaatgcg atgattgtca gcgatttgcc gtttggtgca
    13981 tatcagcaga gtaaggagca ggcgtttgcc gccgccgccg aactgatggc tgccggcgcg
    14041 catatggtta aactcgaagg cggcgtgtgg atggcggaaa cgactgaatt tttgcaaatg
    14101 cgcggtattc cggtttgtgc gcacatcggt ctgaccccgc aatccgtgtt tgccttcggc
    14161 ggatataaag ttcaggggcg cggcggcaag gcgcaggcgt tgcttaacga tgccaaggcg
    14221 catgacgatg cgggggcggc ggtcgtgctg atggagtgcg taccggcgga actggcaaaa
    14281 aaggtaactg aaactgtttc ctgtccgacc atcggcatcg gcgcgggtgc ggattgcgac
    14341 gggcaggttt tggtgatgca cgatatgctc ggcattttcc cgggtaagac ggcgaaattc
    14401 gtcaaaaact ttatgcaggg gcatgatagt gttcaagcgg cggttcgggc gtatgttgcc
    14461 gaagtcaaag ccaaaacttt ccctgctgcg gaacatattt ttgcagattg aacggtttac
    14521 atgcagcatg ccgtctgaaa gccgtttcag acggcatttt gttttgccgt gcgcggcgcg
    14581 tataatcggc gcgttttgtc gggcaggaag cccgaaggat aaggattacc gttatgcaaa
    14641 tcatacatac cattcgagaa ctgcgcgcgt ggcgtaaaaa tgcgggaaag gtggcatttg
    14701 tgccgaccat gggcaatctg catgaagggc atcttgcgct tgtgcgtgag gcgaaaaaac
    14761 gcgcggacag tgtcgtggtc agcattttcg tcaaccgcct gcaattcggt cagggcgagg
    14821 atttcgacaa atatccgcgt actttgcaac aggatgcgga caagctggaa gccgagggtg
    14881 ttgccgttgt tttcgcgccc gatgagaaag aactctatcc gaacgtggaa cagcgttaca
    14941 acgtcgaacc gcccaatctg caaaatgagt tgtgcggcaa gtttcgtccg ggacatttcc
    15001 gcggggtggc aacggtagtg tccaagctgt tcaatattgt cgcgccggat gttgcctgtt
    15061 tcggcaaaaa agactatcag cagcttgccg tgattaaagg tttgaccgag gatttgaatt
    15121 ttgatgttga aatagtgcct gtcgatacgg ggcgcgcgga agacgggttg gcactgtcga
    15181 gccgcaacca gtatttgagt gcggcggaac gcgacgaagc accgcgcctg taccgcgaat
    15241 taaaggctgt tgccgaatcc ttggcgcagg gcagtttgga ttatgcaggt ttggagcggc
    15301 aggctgccga ccatttgacc gctgcgggct gggtggtcga ttatgtcgaa atccgccgcg
    15361 ccgatacgct cgaagtggcg cgggcgggag ataagaaact ggtggtcttg gccgccgcct
    15421 gtctggggac gacgcgcctg attgacaatt tggaaataaa actcccttaa accgcaagcg
    15481 tcgggaatgc cgtctgaagc ggatttgcgt ttcagacggc ggcggattcg catttgaagt
    15541 gcaactttcc ctaacagaaa aaggccagta tgcggtagca tacg
//
LOCUS       DAXHTY010000044        15454 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXHTY010000044 DAXHTY010000000
VERSION     DAXHTY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15454
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.044"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..405)
                     /locus_tag="ACFNMK_001145"
     CDS             complement(<1..405)
                     /locus_tag="ACFNMK_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFC6364425.1"
                     /translation="MPISPVMMPPVTGVPNSHLLFDVAFAVVVLSLLIRGMTIPVMAR
                     LLKVAMPNKPEPKDTHDIWLAKKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFGARC
                     FALIRNGIRIEMQSDTVLQAGIWRGTSCPTARS"
     gene            complement(387..668)
                     /locus_tag="ACFNMK_001146"
     CDS             complement(387..668)
                     /locus_tag="ACFNMK_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364426.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSARLGMPLLLVSPANVLDRA
                     AEALAIAAFLMLVARPSAVFGGLWKFNYSLREKAYISWLGLAEPCRFRR"
     gene            864..1590
                     /locus_tag="ACFNMK_001147"
                     /pseudo
     CDS             864..1590
                     /locus_tag="ACFNMK_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            1659..3197
                     /gene="lnt"
                     /locus_tag="ACFNMK_001148"
     CDS             1659..3197
                     /gene="lnt"
                     /locus_tag="ACFNMK_001148"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC6364427.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGDSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTTRSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3461..4324
                     /gene="rpoH"
                     /locus_tag="ACFNMK_001149"
     CDS             3461..4324
                     /gene="rpoH"
                     /locus_tag="ACFNMK_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC6364428.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(4383..4529)
                     /locus_tag="ACFNMK_001150"
     CDS             complement(4383..4529)
                     /locus_tag="ACFNMK_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364429.1"
                     /translation="MLIHYINFANLSEKQLMRQCLVHVCFLISAIAMAWTDKIVYGTT
                     HKPH"
     gene            4808..5692
                     /locus_tag="ACFNMK_001151"
     CDS             4808..5692
                     /locus_tag="ACFNMK_001151"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC6364430.1"
                     /translation="MSSGNIHIHSMTGFANTAEECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDTENSRQSLELNSDLVNQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRDNTEELTKSITELTEKALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLVEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5776..6291)
                     /locus_tag="ACFNMK_001152"
     CDS             complement(5776..6291)
                     /locus_tag="ACFNMK_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6364431.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLYLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(6366..6785)
                     /locus_tag="ACFNMK_001153"
     CDS             complement(6366..6785)
                     /locus_tag="ACFNMK_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364432.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(6906..7904)
                     /gene="holA"
                     /locus_tag="ACFNMK_001154"
     CDS             complement(6906..7904)
                     /gene="holA"
                     /locus_tag="ACFNMK_001154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC6364433.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVACFDVFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWVVAEDVRTLIRLAAALKQGQSIQSVRNGLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            complement(7906..8385)
                     /gene="lptE"
                     /locus_tag="ACFNMK_001155"
     CDS             complement(7906..8385)
                     /gene="lptE"
                     /locus_tag="ACFNMK_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC6364434.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(8437..9216)
                     /gene="pgeF"
                     /locus_tag="ACFNMK_001156"
     CDS             complement(8437..9216)
                     /gene="pgeF"
                     /locus_tag="ACFNMK_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC6364435.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVDDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTTVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            9383..9496
                     /locus_tag="ACFNMK_001157"
                     /pseudo
     CDS             9383..9496
                     /locus_tag="ACFNMK_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9507..9698
                     /locus_tag="ACFNMK_001158"
     CDS             9507..9698
                     /locus_tag="ACFNMK_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364436.1"
                     /translation="MFQQRTAHQAPHCVLPERDYPPIRFYRHKQTGFNRKGMGIKSIS
                     DISRAMPSENQSPLSDGIV"
     gene            9775..10722
                     /locus_tag="ACFNMK_001159"
     CDS             9775..10722
                     /locus_tag="ACFNMK_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6364437.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMAYLARGNMALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLTIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(10875..11999)
                     /gene="rluD"
                     /locus_tag="ACFNMK_001160"
     CDS             complement(10875..11999)
                     /gene="rluD"
                     /locus_tag="ACFNMK_001160"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC6364438.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIIYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            11998..12801
                     /locus_tag="ACFNMK_001161"
     CDS             11998..12801
                     /locus_tag="ACFNMK_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC6364439.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(12859..15078)
                     /locus_tag="ACFNMK_001162"
     CDS             complement(12859..15078)
                     /locus_tag="ACFNMK_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC6364440.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVFPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQTGIVPSLNASGIRYLDYLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         3755 a         4655 c         3765 g         3279 t
ORIGIN      
        1 cgaccttgcc gtcgggcagg atgtaccacg ccaaatcccc gcctgaagca cggtgtcgga
       61 ctgcatttct attcggatgc cgttgcgtat caatgcgaaa cagcgtgcgc caaacgaatc
      121 ggaaatcggt tcgaccgtat cgggatggtg tccctccgct tcagattcgg caaccacttt
      181 aaacgccgac attctgacaa tttccttttt cgccagccaa atatcatgcg tatctttcgg
      241 ttcgggcttg ttgggcatgg caaccttcaa cagccgcgcc atcacaggaa tcgtcattcc
      301 ccgaatcagc agcgacagca ccaccacggc aaacgccaca tcaaacagca ggtgcgaatt
      361 gggaacgccc gttaccggcg gcatcatcac cggcgaaatc ggcacggctc cgccaaaccc
      421 aaccaactga tatacgcctt ttcacgcagg ctgtaattga atttccacaa accgccgaac
      481 actgccgacg gacgcgcgac cagcatcagg aacgccgcaa tcgccaaggc ttccgccgcc
      541 ctgtccaaca cgttggcagg agaaaccagc agcaaaggca ttcccaaacg tgcggacaaa
      601 gtggtcgaaa ccacgctcag aaacaacagt gcgccacccg gcaggaacag actgcccatc
      661 caatccacgc taacgattct cccttaaaat tcatgaaatt aaaatcgaaa gactgtaaca
      721 cggtattttc ttttcacgaa actttacttc acgtccgcca cagcgttcag acggcatttt
      781 cctgtttcat gccgcctgaa accgcccaaa tccgcgttct cctatataat gtcggacttt
      841 gcgccgcatt tcaggattct ttaatggcat ttgcctcact atttaccctg ccggacgaca
      901 tcaccgccgt tttggacgat gttgccctga tgaccaaagc cgccgcaaag aaaaccgccg
      961 gcgtggtcgg cgacgatttg gcacccaatg ccaaccgtgt tacgggcgta tcggcagaac
     1021 gcgagttgcc gattgtttgg tcgatggcga aaggttcttt ttccaacaag ccggttttgg
     1081 tgcctgcggc cctgctgctt tccgccttcc tgccgcaact cattacgccg cttttgacgg
     1141 caggcggcat ttatctgtgc tttgaaggcg tagaaaagct gctgcacaag tttctgcacc
     1201 gacatgacgc gcatgctcgt aatggcggca atcggcatcg gcatgactgt tttggtgtac
     1261 ggaatcgttg ccgtcatcgt caaactcgac gacttaggca tgctgctgat gcgccgccca
     1321 caaacctttt cgcgcagttt ggggcaaatg ctgactgcct ttatgccttg ctttatgcgc
     1381 ggtttgagcg tggtcggcac gcttgccatg tttcttatcg gcggcgtact ggtcgcccac
     1441 aacctcggcc ttctgcacga tttcctgcac gcgcaacact gggacgctgg ctgggcggag
     1501 tacttcgcca acctcgttgt cgggctgctt tccggttcga ttgcctgcgc ccccgccttg
     1561 ccgctgatga atcgtttcgg caggcattga tttctttttc aaataccgat gccgtttgaa
     1621 agatgttcag acggtatctt ccgaacagac agatgaatat ggtttccaaa ctggacaaat
     1681 actggcagca ccccgccctc tactggcctt tgctcatcct ttttgccgcc gccaccccct
     1741 ttaccttcgc accctactac cacttttggc tgatgccctt gattttcggt gccttcgtcc
     1801 gcctcatcga actgcgtccg cgttttgctg tctcttccgc ctacctgttc ggcctgaccg
     1861 catacacgac acagttctac tggatacaca ccgccctgca cgacgtttcc ggcctgcccg
     1921 acctctatgc cgtaccgctg accttcctac tccccgccta ccttgccctt tatccggcac
     1981 tgtgtttctg gctgtggaaa aaatttaccc tgcctcgggg cataaaaatc ggtttggtac
     2041 tgcccatcct gtggacgctg accgagtttg cccgcgagcg tttcctgacc ggattcggct
     2101 ggggcgcaat cggcgactcc caaatcaccc cggacagccc gctcgccggc tttgccccat
     2161 tgggcggcat ccacatggtt acactggcaa ccgcctttct cggtgtctgg ctggttttgg
     2221 cgagtgataa caccacacgt tcgggcaaac gcctgcttcc aattatcctg attgccgccc
     2281 tgcttgccgc aggctacacc gcccgacaaa ccgacttcac ccgccccgac ggcagccgca
     2341 gcaccgtcgc cctgcttcaa ggcaacatcg accaaaccct caaatggcgt gaagaccaag
     2401 tcatcccgac catacagaaa tattacgaac aggtcggcaa aaccactgcc gacatcgtca
     2461 tcctgcccga aaccgccatc cccgttatgc gccaaaacct gccggaaaac atactggcaa
     2521 aatttgccga acaggcgcaa aacaacggca gcgcgctcgc cgtcggcatc agccaataca
     2581 cttcggacgg caacggttac gaaaacgccg tcatcaacct gaccggttat caggaaaaca
     2641 atcaggacgg catcccctac tacgccaaaa accacctcgt ccccttcggc gaatacaaac
     2701 cgctgccctt cctgaccacg ccgctttaca aaatgatgaa tatgcccctt tccgacttcc
     2761 gcaaaggcgg cggtaagcaa tccgccctgc tgatgaaaaa ccaaaaaatc gccttcaaca
     2821 tctgttacga agacggattc ggcgacgaac tgattgccgc cgccaaagat gccaccctgc
     2881 ttgccaatgc cagcaatatg gcgtggtacg gaaaatccaa cgccatgtac cagcacctcc
     2941 aacaatcgca ggcacgggct atggaactcg gacgctatat ggtccgcgcc accaacaccg
     3001 gcgcaaccgc catcatctcc cccaaaggca acatcatcgc ccaagcccaa cccgatacgg
     3061 aaaccgtatt ggaaggacac atcaaaggct atgtcggcga aacgccctat atgaaaaccg
     3121 gcagttcatg gtggttgatg ggcatattga ccctagccgc actgattctt ttcatcttcc
     3181 gaaacaaaga acactgatac ctgcacagcc cgtccggacg ggctgtcttt tttcaaaccg
     3241 gccgtttaca tacaactacg tatgtaatat aattctgaaa tagaattgaa ccatcaggtt
     3301 tacaaaatca atcaaacttg ccttacaaaa ttttcctgtc tttcaaacct tagcgcatat
     3361 ctcctgccaa acatacaaaa tacaagccaa gcagtgatat actgcatccc aacacagcaa
     3421 accgccgata cgcagtttca gccgaaagga aatgccccaa atgaataacg ctttcgcatt
     3481 acccgccatc caaagcggca acggcagcct cgaacaatac atccacaccg tcaacagcat
     3541 cccgatgctg tcccaggaag aagaaacccg cctcgccgaa cgccggataa agggcgacct
     3601 caacgccgcc aaacaactca tcctgtccca cctgcgcgtc gtcgtttcca tcgcgcgcgg
     3661 ctatgacggc tacgggctga atcaggcaga cctgattcag gaaggcaata tcggactgat
     3721 gaaggcggtc aaacgctacg agcccggtag aggtgcacgc ctgttttcat ttgccgtgca
     3781 ttggattaaa gcggaaattc acgaattcat cctgaggaac tggcgccttg tccgcgttgc
     3841 caccaccaaa ccgcaacgca agctgttttt caatttacgc agtatgcgta aaaacctcaa
     3901 cgccctgtct cccaaagagg cacaagacat cgccgacgat ttgggtgtca aactttccga
     3961 agttctggaa atggaacagc gcatgacggg acacgacatc gccatcatgg cagacaacag
     4021 cgacgacgag gacagtttcg cccccatcga ctggcttgcc gaccacgatt ccgaaccgag
     4081 ccgacaacta tcgaaacagg cgcattacgc cctgcaaaca gaaggtttac aaaacgcttt
     4141 ggcgcaactg gatgacagga gccgccgtat tgtggaaagc cgctggcttc aagacgacgg
     4201 cgggctgacg ctgcaccagc ttgccgccga atacggcgtc tctgccgagc gcatccgcca
     4261 aatcgaagca aaagccatgc agaaactgcg tggtttcctg accgaagaag ccgaagcggt
     4321 ttgaaatata cgcaataaaa tacccgacaa attgttttgt cgggtatttt atttggtaaa
     4381 catcaatgcg gtttgtgcgt cgtgccgtac acgattttat ccgtccaagc catcgcaatc
     4441 gccgaaatca aaaagcagac atgcaccaga cactgccgca tcagctgctt ttccgacaga
     4501 ttggcgaaat tgatatagtg gattaacaaa aatcaggaca aggcggcgaa gccgcagaca
     4561 gtacaaatag tacggaaccg attcactcgg tgcttcagca ccttagagaa tcgttctctt
     4621 tgagctaagg cgaggcaacg ccgtactggt tttggttaat ccactataca tttcccttcc
     4681 ttgcagcaac aaaagcgcgt gattccaatc tgccgctgca aaatcttcaa tttttcacac
     4741 ccgaaaacgc ttgccgccat acagatgtga tatattgatt ccattttaac gggcttcgga
     4801 gaacgacatg agcagcggaa atatccatat ccacagtatg accggatttg ccaatacggc
     4861 agaagaatgc ggcagcaaac gcatcaatct cgacctgcgt gcggtcaacc accgttttct
     4921 agatattcaa atcaggatgc ccgacgattt gcgctatttg gaaagcggta tccgcgagaa
     4981 aatttcatcc catatcgcgc gcggcaaagt cgaatgcaaa atccaaattc aagatacgga
     5041 aaacagcaga cagtccttgg aactcaactc ggatttggtg aaccagcttg ccgaaatcaa
     5101 caaagacctc cgcaagcatc acgatttggc aaaactgggc gttgccgaca tcctgcgttt
     5161 ccccggcgta ttggcaagcc aaagggacaa tacggaagaa ttgacaaaaa gcattaccga
     5221 actgactgaa aaagcgttaa aagactttac tgccgcacgc cggcgcgaag ggaaaaaatt
     5281 gggcgagcac ctgttacaac gcctcgaaag catggaagaa atcatagatg cattgagcga
     5341 actctttcct accctgctgg aaacacataa agaaaaaatc cgcgcccgcc tcgtcgaagc
     5401 agtcggcgac atcaataatg acaggctgca acaggaattt gccctcttta ttcaaaaatc
     5461 cgacatcgac gaagaattca gccgcctgcg cacccacatt gccgaagtgc gccgtatcgt
     5521 taccgaacac aaaggcagca gcggcaaacg gctggacttc ctgatgcagg aattaaaccg
     5581 cgaagccaac actttgggca gcaaatccat tgccgccgaa tgcacccaag cctcggtcga
     5641 actgaaagtc ttaatcgagc agatgcggga acaagtgcag aatatcgaat aatcggtttc
     5701 cccgataagc aaatgccgtc tgaaagccct ttcagacggc attttttcag gttgaaacct
     5761 ttgcggtata aaggcttaag gctttccttt gaccgccacc aatcttgttc ctgcaagaaa
     5821 atcatacaga aactgccgat cgggattcag cagtgcgaac ccccacggta aaatcagcca
     5881 aatcaatgcc gcgccggccg cgcccttggg cggaatgccg aggaagtggc gcaatccggc
     5941 ataagcaagc ataggaatga agacgatgaa tatgcacgcc caaataaagc gcaggcgcag
     6001 caggtacaaa gacggctgta tgccgttaag gtcgcacaag ccgattttcc atgtcctcat
     6061 cgccaaggtc tgcccctgac catgccagtt ggcgcgaaaa taaagccacc atgatcccat
     6121 tatcaggata ctcgttacca atgcagaaac cgcgatagaa acaggattca gaaaaatggc
     6181 ggcaataccg gcaaacaatg ctgccaaaca ggttgccgca ccgaccagca gcatttcgta
     6241 cagcatggcg gcaaagcggc gtttgagcgg ggcggttttc agtatcatca tcttttcaat
     6301 cggaatatgc cgtctgaaac gccaacaggc ttcagacggc attcatgcag atatatctca
     6361 tgtggttaac ggtgcggacg tttctgaccg cgcgactgtt ttgccgccat ttgattgatt
     6421 tcctgacgtg tcgggcgttt tttgaacata gcgtgcgcct cgtccaccga acatttttgc
     6481 gacaggcaga tcagggtaat cgcatctttc tggtgttgtc cgccgctgtg gtacatacgg
     6541 cgcaggtttg cctcgttgat tctgcctttt tcgggatcga tgtcgaattt tttcaggcgt
     6601 tcgataaact ggcgttgctg tcggcgggat gtccaaacaa aaccgcccgc agcggcggcg
     6661 acgatgacgg cggcaagcag gatggcaagc ggccaggaat cgacaatcaa tacgataatg
     6721 aggcttaaaa cggcggtggt cagtaaaatc aggccgccca agcgcagttt ggtcttattg
     6781 tccatagggt taatgttcct ttattgaggt ggtcgtgccg tctgaaatgt tttcggacgg
     6841 attggtgaat acttttcagg attaacggta ttttacagta ttttcggatt ttggggatta
     6901 ccgctttatt ccgccagcga cacgacaagc cgtttgaata ccgtccatgc gtcgccgtct
     6961 tccgcacctt tgatgattcg gtcgatttgg gcgcaggttt tgagcgcgtc aagcaggcgg
     7021 acgacggaaa tccgcttgac cgcaagcggt gcgagcgtct gcttgtcgcc ccaaagcctg
     7081 aggccgttgc ggacggattg gatgctctgc ccctgcttca aggcggcggc aagccggatc
     7141 agcgtccgca cgtcttcggc aaccacccac agaagcagca ccggttcttc gccttcctcc
     7201 tgcaatccgt ccaaaagcct gcatacgcgc gggacatcgc ccttcatcca tgcgccggca
     7261 agctgaaaaa cgtcaaagca ggcaacattg gcaacggcgg tttgcgcctc atcgatattg
     7321 acggtatgcc ctttcggata cagcagcgcg agcttgtcga tttcctgacg cgccgccaac
     7381 aaattgcctt ccacgcgctc ggcaaacagt gccaatgcgt ctgcctcgat acccaaaccg
     7441 attttgtcca gccgtccgcg tatccattgg ggcaaggccg ccgcgccgac cggtttggct
     7501 tcccacactt ccccctttgc cgccaatgcg gcaaaccatt tggactggag ctgggttttc
     7561 tccagtttgg gcagcaaaac cagcgttacc gtatcttccg gcaggcgggc ggcaaaatcc
     7621 tgcaacgcct cgccgccggt tttgccgggt tttccgttgg ggatatgcag ttccaacagc
     7681 ttcaaatcgg caaacagacc cgcactgcct gcggtttgca acagttcgtt ccaatcgaag
     7741 gcattgtctg ccgtataaac ttcccgatta aggtaacctt gtttcttcgc cgccgccctc
     7801 aatgcgtcca atgcctcgat acgcaacagt tcttcctcgc cgtggatgac gtacaggggt
     7861 ttcaaaggcg cgtccgtatc aatgtgtccg atatgtgccg ccacgtcatt ccgccttcag
     7921 aaaggtcagg cggcggacaa tctgttcggc ggcatcctgc cgcatttccg cccacaatgc
     7981 cgcttcctct tcctgtttgc ccaagatctc gttgtcggca taagcaagga cgcggcggac
     8041 ggacacggtc atcggtttgc cgaccggctc gccgcgtttc aatacctgcg cttcaaccgt
     8101 caatatcaaa agatattcgt tgatgactgc cgcacgggta acggtgtagg tttccttgtt
     8161 ttgggaaacg ctgtctatac gcagggtcat ctgcgcgccg gcagcatcgt ccaccctgcc
     8221 cgaagcctga tacagcgcgg tttccaaagg aaactgcaat gcctgtccgc cttcgatgtg
     8281 ccagctccgg taggtcagcg gcggagaaat gccgtctgca cctttcaggt ggaaaccgca
     8341 cgcgcccagc atcagcactg cggcagtaag aaacagtttg ttcatggctt gcctttctca
     8401 aaaacacaaa gtcggtagat tatatcagcg gcgtgttcag acggcattgc cgtccagcca
     8461 aatcaggctc gccatacgcc ctgtcgctcc gtcgcggcgg taggaaaaga aagtatcccg
     8521 ttccaaaacc gtacaatgcg tgccgccata tacgccgccc acgccttcgc gcttcagaat
     8581 caggcgggcg agcgcgtaaa ggtcggcaag gaatttgccg ccacctatgc cttcaaatgc
     8641 ggcggcggct tcgggcatgg gcgtgcagaa cgcatcaaac acgtcctgtc cgacttcaaa
     8701 cgcatccgca ctgatggcgg ggccgagata cgccatcatt tcgacaggcg gaaccttcat
     8761 cgcggcaatg gtgttttgca atacgccgcc cgccaaaccg cgccagcccg cgtgtgcggc
     8821 ggcaaccgtc gtacccgccc tgtcgcaaaa caaaacaggc aggcagtctg cggtcatcac
     8881 ggcacaggca actttgcccg tgtcgtctac ggaagcgtcc gcatcgggtg tgcctcccaa
     8941 cgcttcggca gcattgacga cgacggtact gtggatttga ttgaggtagg caacgggcaa
     9001 tccgacctgc tgttgcacga tttcgcggtt gcggcgcacg gcttcgggat tgtcgtcgac
     9061 gtgcgtaccg aggttcaaac tctgatacgc accttggctc acgccgccgt tgcgcgtggt
     9121 aatcagggtt ttcacattgg cgggcgcggg ccaatcggcg gtcaggaagt ttttgccttt
     9181 cggggcgaga tttagggttt ctgtgatggt tttcattgga tattggaata ggtgtgtggc
     9241 ggaaagggtt gattaactgc cttcggggca aatggatgtt tgttttttcg tcattcccgc
     9301 acaggcggga attcggagat tcgggattgt tttcaaacgt tcgggtttgg attcttgccg
     9361 gtgcgggaat gatgccctta tatatagtgg attaaattta aatcaggaca aggcgacgaa
     9421 gccgcagaca gtacaaatag tacggcaagg cgaggcaacg ccgtactggt ttaaatttaa
     9481 tccactatac tttctccgag ctttatatgt ttcaacagag gacggcacat caagcaccgc
     9541 actgcgtgtt gcccgaacga gactaccctc cgattagatt ctatcgccat aaacagacgg
     9601 gtttcaaccg aaaaggaatg gggataaagt ccatttccga catctctcgg gcgatgccgt
     9661 ctgaaaacca atctccactt tcagacggca ttgtttaggc acggtcattt tgcaaattaa
     9721 ggttaaggcg tgtatgcttg ccttccctta aaaacaaaca cgcaacgcat tattatgaat
     9781 atcctcagta aaatcagcag ctttatcgga aaaacatttt ccctctgggc cgcgctcttt
     9841 gccgccgccg cttttttcgc gcccgacacc ttcaaatggg cggggcctta tattccttgg
     9901 ctgttgggca ttattatgtt cggtatgggt ttgacgctca aaccttccga cttcgatatt
     9961 ttgttcaaac atcccaaagc cgtcatcatc ggcgtaatcg cacaattcgc cattatgccg
    10021 gcaaccgcct ggctgctgtc caaactgttg aacctgcctg ccgaaatcgc ggtcggcgtg
    10081 attttggtcg gctgctgccc gggcggtacg gcttccaatg tgatggccta tctggcgcgc
    10141 ggcaatatgg ctttgtcggt tgccgttacg tctgtttcca ccctgatttc cccattgctg
    10201 actcccgcca tcttcctgat gcttgccggt gaaatgctgg aaatccaagc agccggtatg
    10261 ttgatgtcca tcgtcaaaat ggttttgctc cccattgttt tgggtttgat tgtccataag
    10321 gttttgggca gtaaaaccga aaagctgacc gatgcgctgc cgctggtttc cgttgccgcc
    10381 atcgtgctga ttatcggcgc ggttgttggg gcaagcaaag gcaagattat ggaaagcggc
    10441 ctgctgattt ttgcggttgt cgtactccac aacggcatcg gctacctgct cggcttcttt
    10501 gccgccaaat ggaccggcct gccttatgat gcacaaaaaa cgctgaccat cgaagtcggt
    10561 atgcaaaact cgggcctggc cgccgcgctt gccgccgcac actttgccgc cgcgccggtc
    10621 gttgccgttc cgggcgcatt gttcagcgtg tggcacaata tctccggctc gctgctggca
    10681 acttattggg cggccaaagc cggtaaacat aaaaaaccct aaacgccttt catcataatg
    10741 ccgtctgaac ggggcaaaca aaactggcgg tttctgcccg gccccttcag acggcctttg
    10801 tttgaaatcg gcggggacga ttgcccggct gctccgattc ggctgcctgc gttcgccccg
    10861 cctttcaagc cgcctcactc ccttacataa accacttcga catcatagtc gtcttcgttc
    10921 caatcatcgt cgtcgtccgc gccgaatttg tcctgccatt cttcttcatt gctcaaagac
    10981 gaatccaaac cggcttcaag acggaggacg gacaacaaat gatatatgtc gtccggaatc
    11041 ggtgcctcaa acgaaacggt ttcgccgctt tccggatggg taaaactcaa gcggtaggcg
    11101 tgcaatgcct gacgcgcacc caaactttta acggcttctt tcaccgtgtc gccgcacgga
    11161 tggcgcgggt tgccgtaaac cgggtcgccg gcaagcggat ggttggcttc gcgcatatgt
    11221 acgcggattt ggtgcgtcct gcccgtttcg agcgagcatt cgatatagct gtgggtaaga
    11281 tagcgttcca acactttgac gtgggtaacg gctggtttgc cgccgaattt gacgactgcc
    11341 attttcaggc ggttgtgcgg atcgcgtccg atttgggttt cgattttacc gtcgaagggg
    11401 acgatgccgt tggcgacggc gcggtagatg cgtttgaccg tgcgctcttg aagctgcctc
    11461 acgagggaat tttgcgccga cagggttttg gcaaccacca tcagcccgct ggtttctttg
    11521 tccaaacggt gtacgatgcc cgcgcgcgga atttgggaca gctcggggca atgcgccaac
    11581 aggccgttga gcagcgtccc cgtccagttg cccgccgccg gatgcaccac cagtccggcc
    11641 ggtttgttga cgacgatgac ggtatcgtct tcgtaaataa tatccaaagc cataggctct
    11701 ggaacaaacg ccagattttc ctcactcgga cggacggtta cacaaatttg ctcgccgcct
    11761 atcattttgt ctttgggttg cgaaggttta tcgtttacaa taaccgcgcc ttctttaatc
    11821 catgatgtca ggcggctgcg cgagtagtcg ggcagaagtt tcgccaacac cgcatccaac
    11881 cgcccgccgg caagctcgag cggaacggtc agaccgatac ggttttcagt ttcggaagct
    11941 gacgcaaagt ctgaatcgtc gctataatcg gattcattat caaaggaagt attctgcatg
    12001 aaaaaaattc ttttaacggt ttcattaggt ttggcactga gtgcctgtgc cactcaaggt
    12061 acggtcgata aagatgctca gattacccaa gattggagtg tggagaagct ctatgccgaa
    12121 gcccaggacg agctgaacag cagcaattat acgcgggctg tcaaattata cgaaatcttg
    12181 gaatcgcgct tccctaccag ccgccatgcc cggcaatccc aactggatac ggcatacgcc
    12241 tactataaag atgatgagaa ggataaggcg ctggcggcaa tcgaacgctt ccgccgcctc
    12301 catccgcagc atccgaacat ggattacgcg ctgtatctgc gcggcttggt gctgttcaac
    12361 gaagaccagt ccttcttgaa caaactggcc tcgcaagact ggtccgaccg cgacccgaaa
    12421 gccaaccgcg aagcgtacca ggcgtttgcg gaactcgtcc aacgcttccc gaacagcaaa
    12481 tacgccgccg atgcaaccgc acgcatggtc aaactggtcg atgcactggg cggcaatgaa
    12541 atgtcggtgg cgcgctacta catgaaacgc ggcgcatata tcgccgccgc caaccgcgcc
    12601 caaaaaatta tcggcagcta tcaaaataca cgttatgtcg aagaatcgct cgccatattg
    12661 gaacttgcct accaaaaact cggcaaacca cagcttgccg ccgatacgcg ccgcgtgttg
    12721 gaaaccaact tcccgaaaag cccgtttttg acgcacgctt ggcagcccga cgatatgccc
    12781 tggtggcgtt actggcatta agcccatctt cgtaatgccg tctgaagccg tttcagacgg
    12841 cattttatgt ttcaaacctc attcaaacgg ttttttctgc caatagaatt tataccctct
    12901 caaacgttga gccttcacgc cgccgcgtcc caagccgaat tgcagtgcgc ccgacaaatc
    12961 ggtacgcagc agtttgatgc cgtgtgcgcg gacacggttc tgcaccgctt cggtcggatg
    13021 tttgtaggcg ttggcataac cgcttgaagc aacggcatat tcgggcgaga cggcattgag
    13081 gaacacgccc gacgaggacg tattgctgcc gtgatgtccc aacaccaaaa cctggctgta
    13141 caggttgcct ccatacttgc cgaccaggct ttcctcaccc ttcgtatcca aatcgcccgt
    13201 taccagcagt gccgcaccgc ccgccacaac acgcaaaaca caacttttcc cattatcatc
    13261 gatgtttttg cgttcagacg gcatcaaaaa ctcaaaatcc acgccgtccc attgccaacg
    13321 ctgttccgca caatgccgcg ccccctcata aaattccggc tgtccggcat aaatcccgcc
    13381 gttcggtatc ttgccgactg cctgaaaacc gccgtcgtgg tcgctgtcgt gatgcgacaa
    13441 aaccaaataa tccaaatacc tgattcccga cgcattcagg ctcggcacaa tccccgtctg
    13501 cgccgccgct gccgttccgg tgtcaaacaa aagatgatga tttgccgtct gaaccgacac
    13561 cgacaaaccc tgccccgcat cccaaaccgt aaccgcaacc tcattttccg gcacgccggg
    13621 tgaacggtaa aacacaaacc ctgccaacag caacaccgcc cacggacgca aacccaagcc
    13681 gcgcggcagc agcaacagca aagcggcaca caccgccaac accaacagcg gcaaaggtgc
    13741 ggaggcaacg gcaaactcgg gcgacacatc ggcaagccac accaaaaacc gcaaagtata
    13801 ttccgccaaa aacgcccccg cctgctgaag cggcgcaaac ggaaaaaccg aacccagcaa
    13861 cgccagcggc gtcaataccc aagaaaacca cggaattgcc accgcattga ccaaagggct
    13921 gattaaaggc agcgaagcaa acagataacc gagcaaaacc agcgacaaca ccgaagccgc
    13981 ccactgcccg cgcaaagcag tttgccgttt tccctcgtgc aaacgccccg agcacgccca
    14041 tatcagggcc gccaccaaac cgaaagacag ccaagtcccc acacccaaga ccgccgacgg
    14101 gtcgaacagc agcaccgccg ccaacgcctg ccaccacgtc gcccacgccg acaatcttcc
    14161 cctgcgccaa gcccacgcaa acgccgccaa catcaaaacg ctgcgctgcg tcggcacgga
    14221 aaaaccggca agcagcgcgt aaaacagcgc gcctgcacac cccgccgcca aaacccacgc
    14281 gcgcggccgg gcaggaaggc gcggggaaca ggcaagcagc cgcttcgcca gccacgcaaa
    14341 cattaccgcc accatcgtaa cgtgcaaacc cgaaatactg accaaatgcg tcagccccaa
    14401 cggccggaac gcctgccaca attcggggcg caatgccgac tgttcgccca cgctcaacgc
    14461 gcgcatcagc ccgatgccgt ctgaaagccc gccgtccgca tccgcctgct gccaattacg
    14521 gctgatgcgg ctgcgccaaa ccgcaatccc ccaaccgctt ccgccatgca gcaaaaccct
    14581 gtccgcaccg accgtccccg caccgcctat tccgttggat aatgcccacg cctcacggtt
    14641 caaaccgcgc aggttcaatt ccccgacgac agggtgcaca cgtgccgtta tccgccatct
    14701 gctcccgacc gcccattcgc gccgtttgta gtccgacagc agcaaatcaa acgtccgacc
    14761 accgctgtcc acagcctttg ccgcaaactg cacacgccgc ccgtccgacc tcggcatatc
    14821 cgccacttcc accgtcaacg gcacacctga aaccgcctcc gcccgccatt gcgaagacag
    14881 tgccgcttcc gttctgaata cgccgtaagc cgcgcccgcc aacacgcaca acatcagacc
    14941 ggcaaacgca aaacgccttg caaacacagc aaaaaccgca aaagccgcca gccaaaacag
    15001 ccaatgcggc acgaccggca gcgcaaacga agccgccgcg ccgacaaccc aacacggcaa
    15061 aaaccaacga cggaacacgt ctccccaccc cagatatttc aaaaataaac ggataataaa
    15121 gcaaatccga aaaaccgtca taacaagatt tgaaaatgat atagtggttt aaatttaaat
    15181 caggacaagg cgacgaagcc gcagacagta caagcagtac gacaaggcga ggcaacgctg
    15241 tactggtttt tgttaaacca ctataagaat gcaaacgaaa agcctcagta ttatccgaga
    15301 caacggcaaa ctttccatcg tcattaccac gaacccacat ctcgtcatta ccacgaaccc
    15361 acatctcgtc attaccacga acccacatct cgtcattacc acgaaagcgg gaatctagga
    15421 cgcagggtta agaaaaccta catcccgtca ttcc
//
LOCUS       DAXHTY010000045        15012 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXHTY010000045 DAXHTY010000000
VERSION     DAXHTY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15012
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.045"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..217
                     /locus_tag="ACFNMK_001163"
     CDS             <1..217
                     /locus_tag="ACFNMK_001163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364441.1"
                     /translation="SLHIENAAQANFQFASLVLALSHGERGLGCRLPLRFRQILTSLI
                     PRFSFAEAALSDDLLEHHRYTHLRNAV"
     gene            complement(228..551)
                     /locus_tag="ACFNMK_001164"
     CDS             complement(228..551)
                     /locus_tag="ACFNMK_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6364442.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(630..1286)
                     /locus_tag="ACFNMK_001165"
     CDS             complement(630..1286)
                     /locus_tag="ACFNMK_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC6364443.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1388..1891)
                     /locus_tag="ACFNMK_001166"
     CDS             complement(1388..1891)
                     /locus_tag="ACFNMK_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC6364444.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            2075..2848
                     /gene="tpiA"
                     /locus_tag="ACFNMK_001167"
     CDS             2075..2848
                     /gene="tpiA"
                     /locus_tag="ACFNMK_001167"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC6364445.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2855..3205
                     /gene="secG"
                     /locus_tag="ACFNMK_001168"
     CDS             2855..3205
                     /gene="secG"
                     /locus_tag="ACFNMK_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC6364446.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKYGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3219..3304
                     /locus_tag="ACFNMK_001169"
     tRNA            3219..3304
                     /locus_tag="ACFNMK_001169"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3253..3255,aa:Leu,seq:gag)
     gene            4178..4546
                     /locus_tag="ACFNMK_001170"
     CDS             4178..4546
                     /locus_tag="ACFNMK_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFC6364447.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4543..4854
                     /locus_tag="ACFNMK_001171"
     CDS             4543..4854
                     /locus_tag="ACFNMK_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6364448.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRDFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(5237..5971)
                     /locus_tag="ACFNMK_001172"
     CDS             complement(5237..5971)
                     /locus_tag="ACFNMK_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFC6364449.1"
                     /translation="MNAAAAKDYMKTVELNKSAGNVDTTSRTARRVQAVFRRMLPYAD
                     AANNTGHKFDWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDDEIAAIMGHEMTH
                     ALHEHGKNKVGQQILTNTAAQIGTQIILDKKPDTNPELVGLGMDILGTYGITLPYSRS
                     LEEEADEGGMMLMAQAGYHPAAAVRVWEKMNQENDQNGFISAITSTHPTNNARIENLK
                     RLLPTVMPVYEQSVRNRGRVNKKRRR"
     gene            complement(6196..6309)
                     /locus_tag="ACFNMK_001173"
     CDS             complement(6196..6309)
                     /locus_tag="ACFNMK_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HFC6364450.1"
                     /translation="MRDSDKPAASEGAVKFIEGNPAKKIIAVPGRLVNIVV"
     gene            complement(6302..6472)
                     /locus_tag="ACFNMK_001174"
                     /pseudo
     CDS             complement(6302..6472)
                     /locus_tag="ACFNMK_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(6439..7230)
                     /locus_tag="ACFNMK_001175"
     CDS             complement(6439..7230)
                     /locus_tag="ACFNMK_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC6364451.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFEEKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKIDKLRPTPKAF"
     gene            complement(7519..10149)
                     /gene="leuS"
                     /locus_tag="ACFNMK_001176"
     CDS             complement(7519..10149)
                     /gene="leuS"
                     /locus_tag="ACFNMK_001176"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC6364452.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYGDKENGV
                     LVNSGDLNGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKVDG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVNAFAGSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQF
                     NTVIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            10416..10931
                     /locus_tag="ACFNMK_001177"
     CDS             10416..10931
                     /locus_tag="ACFNMK_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6364453.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNNRGTFPTYDELDLMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEGITTCQYKIIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            10938..11381
                     /locus_tag="ACFNMK_001178"
     CDS             10938..11381
                     /locus_tag="ACFNMK_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC6364454.1"
                     /translation="MPLRLLLGILCDLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11550..13607
                     /gene="ppk1"
                     /locus_tag="ACFNMK_001179"
     CDS             11550..13607
                     /gene="ppk1"
                     /locus_tag="ACFNMK_001179"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC6364455.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDQNVPLLERLCFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAREGIHFYRRRNWTDTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVDRPDLKFPPHTQGRLKALGKNGSIFK
                     LIRRAPILLHHPYQSFNPVVEMMREAAADPAVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            13632..13901
                     /locus_tag="ACFNMK_001180"
     CDS             13632..13901
                     /locus_tag="ACFNMK_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364456.1"
                     /translation="MPSETFPFQTAWFYSNLNRADRSQKHTFAIPAQALPLYAPNPNP
                     PHSRRHYLSDSRTKPRIRLHGSGYISNTGIVFRKTVKSASASQYI"
     gene            complement(13870..14973)
                     /gene="dnaN"
                     /locus_tag="ACFNMK_001181"
     CDS             complement(13870..14973)
                     /gene="dnaN"
                     /locus_tag="ACFNMK_001181"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC6364457.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPHFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
BASE COUNT         3665 a         4224 c         3737 g         3386 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaactttcag tttgccagcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg ataccgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaaat gcctgcctca ataacgcatc
      241 atgttgccgt caatctcggc cgcccatgca tcgatgccgc cctgaaggtt gtacaggttt
      301 tcaaaacccg cgtccgccag atacatcgcc gtatgcaggc tgcggatacc gtgatggcaa
      361 tacaccacaa gcggcacatc atccggcagc tcgttctgcc gcagcggaat cagattcatc
      421 gggatatgca gcgcatttgg cagcgaacaa accgccgttt cttcatccgt acgcacgtcc
      481 aacaaacaaa acatccgccc ttcgtccatc cacgccttca attccgccga cccaagttgc
      541 acaatatcca tcgcaccccc caaaaaaaac caagcaaaat gccgtctgaa gccccaaacc
      601 cgctttcaga cggcatcgca atccgaacat caaaactgaa aaccaccaaa cggatcggca
      661 tccttatcat ccaaatgcgc caccaaggta tcgaacagca ccttctcttc aaacacatcg
      721 cccttgcgcg taatcaaaag cgcgcgctga acaggcttgc ggcctacgat aaccaccatg
      781 cgtccgccat ctttcaactg ttctttcaac acttcaggca caaggtttac cgcaccgccg
      841 acataaaccg catcaaacgg cgcacctgcg gaaagttcgg tcaacccgtt gttttgcaca
      901 taatcgatat tgtccaaacc caagccgtcc aacaccgctt tggcgcggtt ttgctgttcg
      961 gcatcgatgt cgtccgacac cacacgacca gccaattttg ccaacagcgc ggtcgcatag
     1021 cccgaacccg tgccgatttc caaaaccgta tcgtttttcg tcagcttcaa gccctgcgcc
     1081 agccgcgcca cgactttcgg ctcgagcatc ttatgaccgt tggcaagcgg cagcgccata
     1141 tccgcatacg ccaaaccctg caagtcctca tcgacaaaaa gctcgcgcgg aatctccgcc
     1201 aaagcgtcca aaacgtcaaa atccaataca tcccaaggac ggatttgctg ttcgaccata
     1261 ttgaaccgcg ctttttcaaa atccatttag tgctccgtca aataattgtt ccaacaatag
     1321 caggcattat aaaaccacac tcccgccgcg ccaacttcgg cacgcgccgc ccgccccttt
     1381 ccatcactca agccgcctgg cgcgaagcct caaaccgctt ctccaaaatc ttcgccagtt
     1441 cgcccaaata caaagcatct gtctcatcaa actgcgccaa atgttcgctg tccgcgtcca
     1501 acacgccgat acagcggccg tctgaaaaca gcggcacgac aatctccgaa cgcgacaaag
     1561 acgaacaggc aatatggtcg ggatgcgcgt ccacatcccc gacgaccacc gtcccgccct
     1621 tcgcccaagc ctgaccgcac accccgcgac cgaacggaat ccgcgtacac gccaaaggcc
     1681 cctgaaacgg tgccaaaacc aattcgtccg aacgcgtatc gaccaaataa aaacccaccc
     1741 aaaaccaacc gaacgcctcc ttcaaaaccg ccgccgtgtt cgccaaattc gccacccaat
     1801 ccgtctcgtc cgccaccaca gactcaatct gcggcaacac ctcccgataa agcgcggcct
     1861 tgtccgaagc cgaaaaatga agcgcgtgca tcacatctcc tatagttgca tacatatcag
     1921 gcggccatta taaaacaacc tgcccgaaac aacatgccaa accgcccccg cagccgcccg
     1981 ccccaagttg cgaacccgcc gcatatccac taaacttcac attgcaacgc gccacacgcg
     2041 gcagacagaa aaaacacgac acggagcaaa aaagatgtat caccaaatcg gaatgtggga
     2101 tcaaaaatgg gtcatcggca actggaaaat gaacggccgg ctccagaaca acaattcgct
     2161 catgcaccgc ttccgcatcc tccccaccgc cgaacgcgtc ctcatcggac tcgccgcccc
     2221 gaccgtttac ctgctgcaac tgcacaacgc catgcaaatc gtcttaaaca accgcatcct
     2281 cacctgcgcc caagacgtga gccgcttccc cgacaacggc gcgtacaccg gcgaagtgtc
     2341 cgccgaaatg ctcgccgaca ccggcacaga catcgtcctc atcggacact ccgaacgcag
     2401 cctttatttc ggcgaaaaaa acgaaatcca acgccgcaaa atggaaaacg tcctcaacgt
     2461 cggactcatc ccgttattgt gcgtcggcga aagcctcgaa gagcgcgaag ccggcaaaga
     2521 acacgaagtc atcgcccatc agctttccat cctgcaaggg ctggatacca aaaacatcgc
     2581 cgtcgcctac gaacccgtct gggcgatcgg caccggcaaa gtcgccaccg tcgaacagat
     2641 tgccgatatg cacgcattca tctacaaaga aatcttgtct ttgtgcggaa gcgatgttaa
     2701 aatccgcgtc ctttacggcg gaagtgtgaa agcggacaac gcggccgaca tcttcgcagt
     2761 accttatgtg gacggcgcac tcgtcggcgg cgcgtcattg tcgtacgact cctttaccgc
     2821 catcatcagt gccgcacaaa atgcgtagaa aaatatggaa gccttcaaaa ccctaatttg
     2881 gattgttaat ataatttccg ctttggccgt catcgtgtta gtattgctcc aacacggcaa
     2941 aggcgcggat gccggcgcga ccttcggatc gggaagcggc agcgcgcaag gcgtattcgg
     3001 ctctgccggc aacgctaact tcctcagccg ttcgaccgcc gttgcagcaa catttttctt
     3061 tgcaacctgc atggctatgg tgtatattca cacccacacg acaaaatacg gtttggactt
     3121 cagcaacgta caacaaactc agcaagcacc caaacccgta agcaataccg aaccttctgc
     3181 ccctgttcct cagcagcaga aataacagtt tttcaaatgc cgacatggtg aaattggtag
     3241 acacgctatc ttgagggggt agtggccgta ggctgtgcga gttcaaatct cgctgtcggc
     3301 accaacacac aaaacgctta gaaattctaa gcgttttttt cgtttaatcc tgtctaatgc
     3361 cgtcaaccaa tcccaatcaa tacaaggatt tcaggctgat tgttatcctg ccgtccccct
     3421 tcctgacagt gcaatcccgt ccaatccgcc ctaattgaac taacctgatt ttttacagca
     3481 tccgaaaata ccgcgagaaa ataccgtaaa aataaagctg aacgaccacc aaatcaggaa
     3541 tgccaagcga gaaatagctt gcggggaata ctgacgcacc gtagggaatg agggggaaac
     3601 cgtccaagat gcagagcggt tttttttggg tttttggaaa aaacctatac tatagtggat
     3661 taaatttaaa ccggtacggc gttgcctcgc cttgccgtac cggtttaaat ttaattcact
     3721 ataaaataag aaaaagataa aaaattggta acaaatgcgg taacaaatgc ggtaacaaat
     3781 gcggtaacaa atgcggtaac aaatgcggta acaaatgcgg taacaaatgc ggtaacaaat
     3841 ggtaacgaat cggtaacaac ttttggggtt ttccggtttt tcaccgtctt ggcagtggga
     3901 gcgtagcgga atgaaaagcc aaaacgcacg gaaccgcgcc tattttgagc aggaatggcg
     3961 gttaaaccgc ttggttatat atagggggca taggaagaca gcgaaacgcg ctaaaaacgc
     4021 aatttgagcc attaaaagcg attgattaaa aaataatcag gttagccgcc agtgtttcag
     4081 gcggcataaa cggagaaatt gcggggtata aaaaggcggc ttgccgtgtt gtctgtctct
     4141 ggtataattc caagtatcac taatcaacgg ctacacaatg cggatattca aaaaccaatg
     4201 gatagtgaaa tttgccaaga agcacaaaat caacgattcc gagctgctgg aagcggtaga
     4261 gcgggcggat aacgggctga tagacgcaga tttaggcggc ggtgtgatta agcagcgcat
     4321 agcaaggcaa ggacaaggca gaagcggcgg ctatcgcagc ctgatactgt tcaaacaggc
     4381 agacagggca ttttttgttt acgcctttgc caagaacgac agggaaaaca tttcggataa
     4441 agaactggac gtttaccgaa aagccgccgc atattatctg aaatacacgc gggcagagct
     4501 ggcggctttg aaagaagacg gcattatcac ggagatagaa tcatgaaata caaaagcgag
     4561 gcattagccg ccattcatga aatgatggaa ggggcttaca acatcggcgc aatcgacaaa
     4621 aagaccatgc gcgactttga caagtcctgc ctgaccgaaa tcaaaccgtt gagcggcgga
     4681 gacatcaagg caatcaggga gaaggaggca ctatcgcaag ccgctttcgc catctatctc
     4741 aacgtgggaa aaaatcacgt ttcggcttgg gagcggggcg ttaaaaagcc gagcggcgcg
     4801 gcgttgaagc tgctgaccat cgtcaaaaac aagggcatcg aagccattgc gtagacgact
     4861 tggcaaacgg caaaatcagc aagttcacaa tagacacgct gctgaatatg cctgccaaga
     4921 caggcaagac cgccgaaccg aatatcaggg catagccgca caaatgcccg accgcatcaa
     4981 accaagccga aacggcggcg gtgcagacgg catagcccga cagcaaggca cggcgcagac
     5041 gggcgcgaaa cccgaaacat ccccggtcgc aaggcacggg gatttttttg cgcccgttgc
     5101 aggggggatt ggatttaagc ggcgcgggct tgaaggcaaa acgggcgggg ctcagaactg
     5161 tttaaatgcc gtctgaatct caaacggatt cagacggcat tttgaaacaa cggctcaaat
     5221 tctcgatccc cttcccttaa cgccgacgtt ttttattaac gcgcccccta tttctgacac
     5281 tttgctcata aaccggcata acggtcggca acaaccgttt tagattttct atacgggcat
     5341 tgtttgtcgg atgagtagag gtaatagcag aaataaagcc gttttggtcg ttttcctgat
     5401 tcattttttc ccaaaccctg acagcggccg ccggatgata gcctgcctgc gccatcaaca
     5461 tcattccccc ctcatcggct tcttcttcca agctgcggct ataaggcaag gtaatgccgt
     5521 acgttcccaa aatatccata cctaatccga ccaattccgg attagtgtcc ggttttttgt
     5581 ctaatataat ctgcgtgcct atctgcgccg ccgtattggt caaaatttgt tgcccgacct
     5641 tatttttacc gtgttcatgc agggcgtgcg tcatttcatg ccccataatg gcggcaattt
     5701 cgtcatcggt cagcttgagt ttgtcgacta tccccgtata aaacgccatt tttccaccgg
     5761 gcattgccca cgcgttcagc tcatcgtttt tgaaaaccgt cattttccag tcaaacttat
     5821 ggccggtatt atttgccgca tcggcataag gcagcatacg tcgaaatact gcctgcaccc
     5881 tgcgggctgt tctggatgtg gtatcgacat tgccggcaga cttgttcaac tcaaccgttt
     5941 tcatataatc tttggcagcc gcagcgttca ttgtggcgga atcatgaccg taaacatcag
     6001 caacgaccgc acaacctccc aatatcgaga ttgctgccga cagacagact atccgtttaa
     6061 aggaaggaag gaaggaagga aggaaatttc atatttaggt ttactcctta aaaaattaaa
     6121 tttcaaaaaa tgccgtctga atccaaaacg gatttcggac ggcatcttaa cattgtttaa
     6181 tgtttttaaa aagatttaca ccacgatgtt caccagtctg cccggtacgg cgatgatttt
     6241 cttggcagga ttaccttcta tgaatttcac tgcgccttcg gaggcggccg gtttgtcgga
     6301 atcacgcata aattgccaat aattccccga ccttcttacg gctgctgcct tttgcggcaa
     6361 tattgcgctg tacttcaact gttttcagaa tagcagaaga ataaatatcc cttgtgaatt
     6421 cagtatcatg atttgaaatt aaaacgcctt gggagtcgga cgcaatttgt cgattttttt
     6481 gtaaagtccg cgaccaatga attcgatcgt attttggtcg cgcaaaattt gcaactgttg
     6541 gcggattttg tctctgatat ggttgttttg gggaaattgg atggatagtt tttcttcaaa
     6601 ttcatacatt tgcgacaatg tgaattcttc gggaagttgg tcgatacatt tcataacagc
     6661 caaaagccaa cctttgcgct ccgcattttg gttgcgtaaa aacagattgg attgccattt
     6721 tttcagaacg gtttcgggtt cgataatgcg ggaatcgtct attaagaata tttttccgct
     6781 ttcaggcaaa ggggcgagat tgatggaaca cataatgtgg ttcggccggt ttttaatgcc
     6841 tttatttctg ggaataatca tatccggcgt gatgaaatgt ttgggtacaa gcaccaattg
     6901 ccgtatggag taatccgctt ttttatatgc aagaaagaaa aagtttgggt tggtatctga
     6961 ccggatgcgc tccaacatgg tgcgatatgc accgtcaggc acactgttgc ctatggtttt
     7021 ttgattttta ctctttaatt catattgctc gtggcaattt gggcaaaaga ggtctgcaac
     7081 aggtttgtta ttggcaaatc tctgcatcgg cttgcttccg caacagggac agtagccgtt
     7141 tttttccagc caagcctcgc tcattacacg gattttatgg gttgctttat tttgttgctt
     7201 tcccaattcg gtatcgaaaa ataaattcat gttttggatt ttgagattaa gtttgagtgg
     7261 attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacaaatagt acggaaccga
     7321 ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc ggggcaacgc
     7381 cgtactggtt taaagttaat ccgctatatt tgaggtctgt catgcggaca acccaatctg
     7441 ccttaaaaag gccgtctgaa accctgtttc ccagttttca gacggccttt atccgtatgg
     7501 ctaaacctta agagcggttt agacgacgat attcaccaag cgtccgggta cgacgatgat
     7561 tttcttcgcc ggtttgcctt ccatgaattt caccgcgcct tcggtggcga gtgcggcggc
     7621 ttcgaggtcg gctttggatg cgtcggcggc gacggtgatt ttaccgcgca gtttgccgtt
     7681 gacttgaacc atgacttcga tttcggattt gaccaaggcg gcttcgtcga ctgtcggcca
     7741 gcctgcttcc cacagtttcg caccgttcaa ttcgctccac agggtttcgc agatgtgcgg
     7801 cacgatgggc cacagcaggc gtacggcggt ttccaatact tcttgggcga cggcgcggcc
     7861 ttgttcgcca ccggtgtcgg ttttgtcgta ttggttgagc agttccatca cggcggcgat
     7921 gacggtgttg aactgctgac ggcggccgta gtcgtcgctg actttggcga tagtggaatg
     7981 cagtttgtgg cgcaggtctt tgagttcttt agacaaaccg tcttggctgc ctgcgaacgc
     8041 gttgactgct tcgccttgct tcaagtattc gtaaacggta cgccacaggc ggcgcaggaa
     8101 gcggtgcgcg ccttcgacgc cgctgtcgct ccattcgagg gactgttcgg gcggtgcggc
     8161 gaacatcatg aacaggcggg cggtgtctgc gccgtaggcg ttaatcagtt cttgcggatc
     8221 gacgccgttg tttttggact tggacatttt ttccgtgccg ctgatgacga cgggcagtcc
     8281 gtcgactttc aggacggcgg aaacagggcg gcctttgtcg tcaaaagtca gctcgacatc
     8341 ggcggggttg atccaatctt tgccgccttt gtcgttttcg cggtagtagg tttcgcaaac
     8401 gaccatgcct tgcgtcagca ggcgttcaaa cggttcgtca acattgacca aaccttcgtc
     8461 gcgcatcagt ttggtgaaga aacgcgcgta caagaggtgc aaaatcgcgt gttcgatgcc
     8521 gccgatgtat tggtcgaccg cgccccagta tttcgcggcg gcaggatcga ccatgccgtc
     8581 tgaaaatttt ggcgacatat agcggaagaa ataccagctc gattccatga aggtgtccat
     8641 ggtgtcggtt tcgcgttttg ccgcgccgcc gcagcatggg caggcagttt cgtaaaactc
     8701 gggcattttt gccagcggcg aacccatgcc gtcgggtacg acgttttcag gcaaaacgac
     8761 cggcaattgg tcggcaggga cgggtacgtc gccgcattgt tcgcaatgga cgatgggaat
     8821 cgggcagccc cagtagcgtt ggcgcgaaat gccccagtcg cgcaggcggt attgggtttt
     8881 cggttcgccc gcgccttggc tttgcagctt ggcggcgacg gcgtcgaatg ccgtctgaaa
     8941 atccaagccg ttcaagtcgc cgctgttgac caatacgccg ttttctttgt cgccgtacca
     9001 ttcttgccat cggtttgcgt cgaatgcgtt gtcgccgacg gcaatgactt gttttttagg
     9061 cagattgtat ttggcggcga actcgaaatc gcgttcgtcg tgcgccggaa ccgccattac
     9121 cgcgccgtcg ccgtagcccc acaatacata gttggcaatc cacacttcca gcttgtcgcc
     9181 gttgagcggg ttgacgacgt agcggccggt cggcacgcct tttttctcca tcgtcgccat
     9241 atcagcttcg gcaaccgaac cggctttgca ttcggcaata aatgcctgca attcgggttt
     9301 gtcggcggct gcggcggttg ccagcggatg ctcggcggca acggcaacat aagtcgcgcc
     9361 catcagcgtg tcggggcggg tggtgtaaac ttgcaggaat ttcgcgtaat cgccttccaa
     9421 gccttgtttg ctgtcgtctg aaacggcgaa gcgcacggtc ataccgcgag atttgccgat
     9481 ccagttgcgc tgcatggttt tgacttgttc aggccagtgt tccagtttgt ccaagtcgtt
     9541 gagcagctct tcggcgtaat cggtgatttt gaagtaatac atcgggattt cgcgtttttc
     9601 gattaacgct cccgaacgcc agccgcgtcc gtcgatgact tgctcgttgg caaggacggt
     9661 ttggtcgacc gggtcccagt tcaccgtgcc gtttttgcga tagacgatgc ctttttcaaa
     9721 cagcttggta aacagccatt gttcccagcg gtagtattcg ggtttgcagg ttgcggtttc
     9781 gcgcgcccag tcaatcgcaa aacccaggct tttgagctgg gttttcatgt attcgatgtt
     9841 atcgtacgtc caagcggcag gggcgacgtt gtttttcatc gccgcgtttt ccgccggcat
     9901 gccgaacgcg tcccaaccca taggctgcat gacgttgaag ccgtttaaaa gtttgaagcg
     9961 gctcaataca tcgccgatgg tgtagttgcg cacatgcccc atgtgcagct tgccgctggg
    10021 ataggggaac atggagaggc aataatattt gggtttggaa gcgtcttcgg agacgttgaa
    10081 aatacgggcg tcgtcccatt ttttctgcgc cgcaggctcg atggcggcgg gttggtaatg
    10141 ttcttgcatg gtcattctgt ttttcgcttg aaaacgttag gaatttaaag gaggaattat
    10201 aacaggttgc cgatgtcggc gaatcggcaa attgccggca ggatgcgtaa attcgcacgc
    10261 gcatcattcc gtatgccgta caaatacacc gcgtttactg atacgcacgt tttttatgct
    10321 aatattacaa accaaaatca aatgtttaaa actctcctga tgcggctctt ccgaacaaaa
    10381 ggcagacggg catcgggtaa aagaggattc tgcatatgaa aatcaaacaa atcgtcaaac
    10441 cgggcttggc agtattggcg gcgggcgttc tgtctgcctg cgcaaccaaa agcaacgtca
    10501 aagccgacgg aacgaccgac aatccggttt tcccgaaacc ctattccgta acgctcgaca
    10561 acaatcgcgg tacattcccg acctatgacg aattggactt gatgcgtccc ggtctgacca
    10621 aagacgacat ctacaaaatc ctgggtcgtc cgcattacga cgaaggtatg tacggcgtgc
    10681 gcgaatggga ttatctgttc cacttccaca ccccgggcgt aggcatcgac cctgaaaaca
    10741 cttccggcgt agaaggcatt accacctgtc aatacaaaat tattttcgat aaagacaaat
    10801 ttgcccgcag cttctactgg aaccccgtct tcccgaaaga tgccgcctgt ccgccgcccg
    10861 cacccaaagc cgagccgcaa gtcatcatcc gcgaaatcgt gcccgccaaa cccaaacgca
    10921 tccgccaata agccgctatg ccgttgcgcc tgcttttagg gatattgtgc gacctatcga
    10981 tggttgcccc cgtatatgca caggggcagc cggatgcggt caatgcctat atccaaaaga
    11041 aaaaagtcat cgtcaataca tccaaagcgg aactctgttt cgctgacgac cgtcagtgcc
    11101 accccgtcct catcggtgtt gccacgccca aggggacgtt cgggctgacg ctgaacagta
    11161 ccgacaagcc cggatacggc ggcgaagtca tcggtttcaa gcaggagggt gattttcttt
    11221 tcgccctgca ccgcgtttgg aatcagatac cgtcggaaag gcggaacgaa cgcatcgcct
    11281 ccccgtccgt gtccgacagg attatgacca acggctgcat caacgtcagc gatgcggtgt
    11341 acgaaaaact gcgtcattat tttgtgttgg aagtgatttg aaacagacgg ataccgcacg
    11401 cgccggtatc tgttttcaca ttgccccgat gcctgaaaca gactgtccgc cacgtcatgc
    11461 cgtctgaaac cggcgcagat gccgccaagc cttcagacgg cattgcctgc ccgctccgac
    11521 cgaacaacaa ccatctttgg gagaacctta tgcccgaaca aaaccgcatc ctctgccgcg
    11581 aactgagctt gctggcattc aaccgccgcg tgttggcgca ggcggaagac caaaacgtcc
    11641 cccttttgga acgcctgtgc ttcctgtgca tcgtttcatc caacctcgac gagtttttcg
    11701 aagtccgcat ggcgtggctc aaacgtgaaa acaagctgca cccccggcgc aggctggaca
    11761 acggcaaaat gccgtctgaa accatcgccg acgttaccga agcggcgcgc tccctgatac
    11821 ggcaccagta cgacctgttc aacaacgtcc ttcagcccga gttggcgcgg gaaggcatcc
    11881 atttttaccg ccgccgcaat tggacagaca cacagaaaaa atggattgaa gactattttg
    11941 accgtgagtt gttgccgatt ctgaccccca ttggccttga cccgtcccac cccttcccgc
    12001 gaccgctgaa caaatcgctc aacttcgccg tcgaactcga cggcacggac gcgttcggca
    12061 ggccttcggg gatggcgatt gtgcaggcac cgcgcatcct gccgcgcgtt gttcccctgc
    12121 cgtccgaact gtgtggcggc ggacacggct tcgtcttcct gtcgtccatc ctccacgccc
    12181 acgtcggcaa actcttcccg ggcatgaacg tcaaaggctg ccaccagttc cgcctcacgc
    12241 gcgacagcga cttgaccgtt gacgaagaag acgtacaaaa cctccgcgcc gccattcaaa
    12301 acgagttgca cgaccgcgaa tacggcgacg gcgtgcggct cgaagtcgcc gacacctgtc
    12361 ccgcctacat ccgcgacttt ctgctcgcgc aattcaaact gaccgccgcc gaactctatc
    12421 aggtcaaagg cccggtcaac ctcgtgcgcc tcaacgccgt ccccgatttg gttgaccgtc
    12481 ctgatttaaa atttccgccc catacacaag gcaggttgaa agccttgggt aaaaacggct
    12541 cgatattcaa actgatacgc cgcgcaccca tcctgctgca ccacccctac caatccttca
    12601 atcccgttgt cgaaatgatg cgcgaagccg ccgccgaccc cgccgtgctt gccgtcaaaa
    12661 tgacgattta ccgcaccggc acgcgttccg aactcgtccg cgccctgatg aaggcggcac
    12721 tcgccggcaa acaagtaacc gtcgtcgtcg aactgatggc gcgttttgac gaagccaaca
    12781 acgtcaactg ggcgaagcag ctcgaagagg cgggcgcgca cgtcgtgtac ggcgtgttcg
    12841 gctacaaagt ccacgccaag atggcactgg tcatccgccg cgaagacggc gtactcaagc
    12901 gttacgccca cctcggcacg ggtaactacc accaaggcac atcgcgcatc tacaccgact
    12961 tcggcatcat taccgccgac gaacaaatca ccgccgatgt gaacacattg tttatggaaa
    13021 tcacaggctt gggcaaaccc gggcggctga acaaactcta ccaaagcccg tttaccctgc
    13081 acaaaatggt tatcgaccgc atcgcacgcg aaaccgaaca cgcaaaagcc ggcaaaccgg
    13141 cgcggattac cgccaagatg aattcgctca tcgaaccgac cgtcatcgaa gccctgtatc
    13201 gggcaagcgc ggcaggcgta caaatcgatt tgattgtgcg cggtatgtgc accttgcgcc
    13261 cgggtgtaaa aggcttgtcc gaaaacatcc gcgtccgctc catcatcggc aggcagctcg
    13321 aacacgcgcg cgtgtattgc ttccacaaca acggcgcgga cgataccttt atctccagcg
    13381 cggattggat ggggcgcaac ttcttccgcc gcatcgaaac cgccgcgccg attaccgcgc
    13441 ccgaactcaa aaaacgcgtc atccgcgaag gtttggaaat ggcattggca gacaatacgc
    13501 acgcgtggct gatgcagccc gacggcggct atatccgcgc cgcgcccgcc gagggcgaat
    13561 ccgaagccga cctgcaaaac gatttgtgga cactgctcgg aggctgaccc gcaccgcccc
    13621 aatcaaaaac catgccgtct gaaacctttc cgtttcagac ggcatggttt tacagcaatc
    13681 taaacagggc ggaccggagt caaaaacaca ccttcgccat tcctgcacaa gcacttcccc
    13741 tatacgctcc caaccccaat ccgccgcatt ccagacggca ttacctttcc gacagccgaa
    13801 caaaaccccg aatccgtctg cacggttcgg ggtatatctc caatacgggc atcgtgttcc
    13861 ggaaaaccgt caaatccgca tcggcatcac aatatatttg aaattcggat tgttcggcac
    13921 ggtaaacagc gtcgagcggt tggcatcgcc gaaggcaagc tgcatatcgt cggaatggat
    13981 gttgcgcaac acgtccatca gatagccgat attgaaaccg acttcgagtt cgccgccctg
    14041 ataggcgatt tcgatttctt cgcgcgcttc ttcctgctcg ttgttgctgc acacaacgct
    14101 caacaggccg ggttgcagga acaggcgcgc gccacggaat ttttcgttgg caagaatgga
    14161 cacacgttcc agcgcgccca aaagttcggc acgggacaaa acgaaaatct tgtcgttgtc
    14221 caaagggatg acgcggttga aatgggggaa tttgccgtcg atgaccttgc tgacgatggt
    14281 cgtgccgttg cattggaaac gcacctgttt gtccagcagc tcgatttgaa tcggatcgtc
    14341 ggggttgttc aacagtttga acagttccag caccgttttg cgcggcaaaa tcacttcggc
    14401 gcgcggcaaa tccgcatcaa tcgcgcaggc tgcataggca aggcggtgtc cgtcggtcgc
    14461 cacaaggcgc aactggctgc cctcaacctg catcagcaga ccgttgagat aatagcggat
    14521 gtcctgcacc gccatgctgt actgcacttg cgacagcatg gttttgaaac gctcctgctc
    14581 cagcgagaaa gtcgcgctga tgtcctcgcc gacattcatc atcggaaaat cggcggcagg
    14641 cagggtctgc agggcaaaac gcgatttgcc cgctttcagc gtcagacggc tgtcgtccca
    14701 atccagcgac accagcgcac cggcaggcag tgcccgtaaa atatcctgaa atttcttggc
    14761 attggtggtg atgcggaagt cgcccgcgcc gccctcggga cccgcagtgt cgatttggat
    14821 ttccaaatcg gttgccaaga gtttggtctg accgcctttt ccctcaatca ggacgttgga
    14881 caggatgggc agggtgtggc ggcgttcgac gatgccggta acggcttgca gcggcttgag
    14941 caggctgtcg cgttcggctt gtaaaatcaa catgttcgct cctttaaatc ggtttgtata
    15001 gtggattaaa tt
//
LOCUS       DAXHTY010000046        14882 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXHTY010000046 DAXHTY010000000
VERSION     DAXHTY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14882
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.046"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..390
                     /locus_tag="ACFNMK_001182"
     CDS             <1..390
                     /locus_tag="ACFNMK_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC6364458.1"
                     /translation="GRLPQLSVFGGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGV
                     AGVHLFNLGSGRAYSVLEIIRAFEAASGLNIPYQIQPRRAGDLACSFADPSHTKQQTG
                     WETKRGLQQMMEDSWRWVSRNPNGYGD"
     gene            391..1473
                     /gene="rfbB"
                     /locus_tag="ACFNMK_001183"
     CDS             391..1473
                     /gene="rfbB"
                     /locus_tag="ACFNMK_001183"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC6364459.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIDSAGEF
                     IQTNIVGTFNLLEAARAYWQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYA
                     PSSPYSASKVSSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKP
                     LPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTWWQNVLNGSYRLERLGTGK"
     gene            1536..2402
                     /gene="rfbA"
                     /locus_tag="ACFNMK_001184"
     CDS             1536..2402
                     /gene="rfbA"
                     /locus_tag="ACFNMK_001184"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC6364460.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEITDLNRMYLEDGSLSVQLLGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            2456..2995
                     /gene="rfbC"
                     /locus_tag="ACFNMK_001185"
     CDS             2456..2995
                     /gene="rfbC"
                     /locus_tag="ACFNMK_001185"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6364461.1"
                     /translation="MNIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWVGEILS
                     AENKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPKAEHSLIWNDPTVGIDWPLQTA
                     PNLSPKDLAGKVLAEAVTF"
     gene            3019..5133
                     /locus_tag="ACFNMK_001186"
     CDS             3019..5133
                     /locus_tag="ACFNMK_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFC6364462.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLFTLLPEF
                     HICKDGKGAEAVIGWGLRPTTHKARAFAAEHQLPFIALEDGFLRSLGLGVAGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQAMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLAQQHRVNLLAEDINPISLLQNIDKVYCVTSQMGFEALLCGKPLTT
                     FGLPWYAGWGVSDDRHPKIDSLVQTQRRAPRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLAGVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMGI
                     YFNAEAPSRLEHILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     DDAARYADQTAEQADILTCLQYADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLVAGTLIYYPDYIHPKTHQAINAETAAQILIRQKNMQKNNN
                     GLHRGCFAKKLGKIKQLYRSFK"
     gene            5270..6529
                     /locus_tag="ACFNMK_001187"
     CDS             5270..6529
                     /locus_tag="ACFNMK_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFC6364463.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVRGGFTPMAKNAIRYYIELFRNLRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELQGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSDSSTPAT"
     gene            6896..8302
                     /locus_tag="ACFNMK_001188"
     CDS             6896..8302
                     /locus_tag="ACFNMK_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6364464.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            8520..9734
                     /gene="gltS"
                     /locus_tag="ACFNMK_001189"
     CDS             8520..9734
                     /gene="gltS"
                     /locus_tag="ACFNMK_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC6364465.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            9833..10849
                     /locus_tag="ACFNMK_001190"
     CDS             9833..10849
                     /locus_tag="ACFNMK_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6364466.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            10860..11057
                     /locus_tag="ACFNMK_001191"
     CDS             10860..11057
                     /locus_tag="ACFNMK_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364467.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            11075..11224
                     /locus_tag="ACFNMK_001192"
     CDS             11075..11224
                     /locus_tag="ACFNMK_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364468.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAVFSCVSYADVSTHKTAHKAL
                     PYVPS"
     gene            complement(11483..12883)
                     /locus_tag="ACFNMK_001193"
     CDS             complement(11483..12883)
                     /locus_tag="ACFNMK_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFC6364469.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKNGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNERHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGAAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTTYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
     gene            complement(13193..14665)
                     /gene="pyk"
                     /locus_tag="ACFNMK_001194"
     CDS             complement(13193..14665)
                     /gene="pyk"
                     /locus_tag="ACFNMK_001194"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC6364470.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     LLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT         3546 a         4157 c         3747 g         3432 t
ORIGIN      
        1 ggcaggctgc cgcaactgtc ggtattcggc ggcgattatc cgacccccga cggtacggga
       61 atgcgcgact acattcatgt gatggatttg gcagaagggc atatcgcggc aatgaaggca
      121 aaaggcggcg ttgccggcgt acatttgttc aacttgggtt cggggcgcgc ctattccgtt
      181 ttggaaatca tccgtgcctt tgaagccgca tccggcttaa atattcccta tcaaatccaa
      241 ccccgacgcg ccggagactt ggcgtgttcc tttgccgacc cgtcccatac caaacaacaa
      301 accggctggg aaaccaaacg cggcttgcag caaatgatgg aagattcgtg gcgttgggtc
      361 agccgcaacc ccaacggata tggggattaa atgcaaaccg caaacaaaaa aaccatcctt
      421 gttaccggcg gtgcgggctt tatcggttct gccgttgtcc gtcatattat ccgaaacacc
      481 caagattccg tcgtcaacct cgacaaactg acctacgcgg gcaatctcga atcgctgacc
      541 gacattgccg ataatccccg ctatgctttt gagcaagtgg atatctgcga ccgcgccgaa
      601 ctcgaccgcg tattcgcgca acaccggcca gatgcggtga tgcacttggc agcggaaagc
      661 catgtcgacc gctctatcga ttcggcaggc gagtttatcc aaaccaatat cgtcggcaca
      721 ttcaatctgc ttgaagcagc ccgcgcctac tggcaacaaa tgccgtctga acagcacgaa
      781 gccttccgtt tccaccatat ttccaccgat gaagtctatg gcgatttagg cggcacggac
      841 gatttgttta ccgaaaccgc gccctacgcg ccgtccagcc cctactctgc ctctaaagtg
      901 tccagcgacc acctcgtccg cgcgtggttg cgtacttacg gcttgccgac cattgtaacc
      961 aactgctcca acaactacgg tccttaccat tttccggaaa aactcattcc tttgatgatt
     1021 ctgaacgcgc ttgacggcaa accgctgcct gtgtacggcg acggtatgca aatccgcgac
     1081 tggctgtttg tcgaagacca cgcgcgcgca ctgtatcagg ttgttaccga aggtgttgtc
     1141 ggcgaaacct acaatatcgg cggccacaat gaaaaagcca atattgaagt cgtcaaaacc
     1201 atctgcgccc tgctggaaga actcgctccc gaaaaaccgg ccggtgtggc gcgttatgaa
     1261 gatttgatta ctttcgtaca agaccgcccc ggccatgacg tacgctacgc cgtcgacgca
     1321 gccaaaatca ggcgggattt gggctggctg cctttggaaa ccttcgagtc cggcctccgc
     1381 aaaaccgtgc aatggtatct ggacaacaaa acctggtggc aaaatgtatt gaacggcagc
     1441 tatcgtttgg aacgtttagg tactggaaaa tagttttcag acggcatccc gacgcaatgc
     1501 cgtctgaaaa cccatcgcaa aggaagaaag aaaagatgaa aggcatcata ctggcaggcg
     1561 gcagcggcac gcgcctctac cccatcacgc gcggcgtatc caaacagctc ctgcccgtgt
     1621 acgacaaacc gatgatttat taccccttgt cggttttgat gctggcggga atccgcgata
     1681 ttttggtgat taccgcgcct gaagacaacg cctctttcaa acgcctgctt ggcgacggca
     1741 gcgatttcgg catttccatc agttatgccg tgcaacccag tccggacggc ttggcacagg
     1801 catttatcat cggcgaagaa tttatcggca acgacaatgt ttgcttggtt ttgggcgaca
     1861 atatttttta cggtcagtcg tttacgcaaa cattgaaaca ggcggcagcg caaacgcacg
     1921 gcgcaaccgt gtttgcttat caggtcaaaa accccgaacg tttcggcgtg gttgaattta
     1981 acgaaaactt ccgcgccgtt tccatcgaag aaaaaccgca acggcccaaa tccgattggg
     2041 cggtaaccgg cttgtatttc tacgacaacc gcgccgtcga gttcgccaaa cagctcaaac
     2101 cgtccgcacg cggcgaattg gaaattaccg acctcaaccg gatgtatttg gaagacggtt
     2161 cattgtcggt acaactgttg ggacgcggtt tcgcatggtt ggacaccggc acgcacgaaa
     2221 gcctgcacga ggcggcttca ttcgtccaaa ccgtgcaaaa catccaaaac ctgcacatcg
     2281 cctgcctgga agaaatcgct tggcgcaacg gttggctttc cgatgaaaaa ctggaagaat
     2341 tggcgcgccc gatggcaaaa aaccaatacg gccaatattt gctgcgcctg ttgaaaaaat
     2401 aatgtttgag gccgtctgaa acttttcaga cggcctttag atgaaagata aaaagatgaa
     2461 catcatcgac accgccctcc ccgacgtaaa acttttaaaa ccgcaagtct tcacagacgg
     2521 gcgcggcttt tttatggaaa ccttccgcga cgggtggttc aaagaaaata ttgccgaccg
     2581 aaccttcgtg caggaaaacc actccaaatc cggcaaaggc gtattgcgcg gcctgcacta
     2641 tcaaactgaa aacacacaag gcaaactcgt acgcgtggtt gtcggcgaag tattcgacgt
     2701 ggccgtcgat atgcgcgaag gttcgccgac tttcggcaaa tgggtaggcg agattctgtc
     2761 cgcagaaaac aaacgccaac tgtgggtacc cgaaggtttc gcacacggct tctatgtact
     2821 gagcgatgaa gccgagttcg tctataaatg cacagactat tacaacccca aagccgaaca
     2881 ctcactaatt tggaatgatc cgaccgtcgg catcgactgg ccgcttcaaa ccgcgccgaa
     2941 cctgtcgcct aaagacttgg caggcaaagt attggccgaa gcagtcacgt tttaagacta
     3001 ttcaggccgt ctgaaagaat gttcctcttt tcagacggcc tacaatccat taataacaat
     3061 aatcgacgaa aacgcattgt gaaaaacgcc tacatcccct ctcgtggcat ccgcaaaatc
     3121 ccccatctct tcaccctatt gcctgagttt catatctgca aggatgggaa aggagcagag
     3181 gctgttatcg gctggggttt gcgcccgacg acacacaaag cgcgtgcttt tgccgctgaa
     3241 caccagcttc cctttattgc tttggaagac ggctttttac gctcgcttgg cctgggcgtt
     3301 gccggctatc ctccctattc catcgtctat gacgacatcg gcatctacta cgacactacc
     3361 cgcccttctc gtttggaaca actgattctt gccgccgata ccatgccgtc tgaaaccttg
     3421 gctcaggcgc agcaggcgat ggatttcatc ctgcaacacc acctgtccaa atacaaccac
     3481 gcgcccgaac tttcagacga tcatccttta cgctccccat ccaaacctga aaccgtcctc
     3541 attatcgacc aaacattcgg cgatatggcc atccaatatg gcggcgcaga cgcctctacg
     3601 tttgaactga tgtttcagac ggccttaaat gaaaacccgc aagccgatat ttgggtcaaa
     3661 acccatcccg atgttttgtg cggcaaaaaa caaggctatc tgacccaact ggcgcagcaa
     3721 caccgcgtca atcttctggc agaagacatc aatccgattt ctttgttgca aaacatcgat
     3781 aaagtttatt gcgttacctc gcaaatgggt tttgaggcgc ttttgtgcgg caagccgctg
     3841 accactttcg gcctgccgtg gtatgccgga tggggcgtga gtgacgaccg ccatcctaaa
     3901 atcgacagcc ttgttcaaac ccaacgccgc gccccccgca acttgctgca gctcttcgct
     3961 gcagcctatc tgcaatacag ccgctacctc aaccccaata ctggcgaagc aggcagcctc
     4021 tttgatgtca tcgactatct ggcgacggtc aaacgcaaaa acgacaaatt gcgtggcgag
     4081 ttgtattgcg ttggtatgtc tttgtggaaa cgcgcggttg ccaaaccgtt ctttaacgta
     4141 ccctcttgcc gtctgaaatt tatctcttcc acccaaaaac tggcaggggt caaactgtcc
     4201 gacgatgcac gcatccttgc ctggggcaac ggcaaagagg ccatcgtccg ctttgccgaa
     4261 caacaccaca tccccctgct gcgcatggaa gacggcttta tccgatcggt cggactcggc
     4321 tctaatttag taccgccact gtcacttgtt accgacgata tgggcattta tttcaatgcc
     4381 gaagccccat cccgtcttga acacatcctg caaaaccaaa acttcgacga tcaagacttt
     4441 cagacggcct tgaagctgca aaaaatgctg accgaaaacc acatcagtaa atacaacgtc
     4501 ggcagctcag acttcaccgc cccgtcaacc gacaaaaccg tgatcctcgt tcccggccag
     4561 gttgaagatg atgcgtctat ccgctacggt tcgccccaaa tctaccgcaa tctggatttg
     4621 ctccgtaccg tacgcgaacg aaaccccaat gcctatatca tctacaaacc gcatcccgat
     4681 gtagtcagcg gtaaccgcat cggccatatt tcccctgatg atgctgcacg atatgccgac
     4741 caaaccgccg aacaagccga catcctgacc tgtctccaat acgcagacga aatacatacc
     4801 atgacttcgc tgaccggttt tgaagccttg ttgcgcggca aaaaagtcag ctgctacggc
     4861 ctgcctttct atgcaggttg ggggcttacc caagatctgc tccctattcc gcgccgcagc
     4921 cgcaggcttg agctttggca gctggttgcc ggcacgctca tctactatcc cgactacatc
     4981 caccccaaaa cccatcaggc catcaatgcg gaaactgcag cccaaatcct gatacgacaa
     5041 aaaaatatgc aaaaaaacaa caacggatta catcgcgggt gctttgccaa aaaattaggt
     5101 aaaatcaaac aactatatcg atctttcaaa taaataccat caaagttaac gatgcgtcat
     5161 aaacttgcct ctattgcggc atcattgcct ttgcatcgtt aattctcttg gcgtatgctt
     5221 gaaagttcaa cctaaaacta ttacataaaa aacaaaacca cattgcaaca tgaaacagac
     5281 cgtcctcaaa aataacctgc aaaacctgct tgaaagcgca gaaaatatcc tgctgcttca
     5341 aggccctgtc ggcgattttt ttctgcgcct tgccgactgg ctgactgcaa acggcaaaac
     5401 cgtacataaa ttcaacttta atgcaggcga cgactatttt tatccgccca ctcaagcgca
     5461 taccgttgtt tttaacgaca actacgatgc ctttcctgag tttttgcaag aatacatcac
     5521 tcaacatcac atccaggccg ttgtctgctt tggcgacaca cgcccttatc acgtcattgc
     5581 aaaacgcatt gcaaacgaaa accaagccag tttctgggcg tttgaagaag gctatttccg
     5641 cccctactac atcaccttag aaaaagacgg cgtcaacgca ttttccccgt tgccacgccg
     5701 tgccgacttt tttcttgaac aattccctaa gcttgcccag caagaatata aagcgccaac
     5761 gccggtacgc ggcggattta cgcccatggc aaaaaacgcc atccgttact atatcgagtt
     5821 gttccgcaat ctacgcaaat accccgacta catccaccac cgcgcaccca atgccggcca
     5881 ttacctcaaa ccgtggtcgc tctccatcct caagcgtttg aactactata ttgaagacat
     5941 ccaaatcgcc aaacgtgtgg aagcaggcaa atacggcaag ttttttattg ttcccttgca
     6001 ggtattcaac gacagccaag tccgtatcca ttgcgacttt cccagcgtcc gcagcttcct
     6061 gctccatgtt ttgagttcat ttgccgagca cgcgcctgcc gataccaaca tcatcatcaa
     6121 gcatcatccg atggaccgcg gttttatcga ctactggcgc gacattaaac gctttatcaa
     6181 agaacacccc gaactccaag gccgtgtgat ttatgtccat gatgtccccc tgcccgtttt
     6241 cctgcgccac ggtctcggca tggttaccat caacagcacc agcggcctgt ccggactgat
     6301 tcacaatatg ccagttaagg ttctcggccg tgcctattat gatattcccg gtattactga
     6361 ccaaaatacc ttggcagaat tttggaatca tccgacaccg cctgacaaag agctgttcca
     6421 tgcctaccga atgtaccacc tcaacgtgac ccaaattaac ggcaacttct acagtcaggt
     6481 cttttttcct aacaaaaaaa cctccgactc ttccactcca gcaacctgac ttagcaaaaa
     6541 taattcaggc cgtctgaaaa catttcagac ggcctgaaac aatcaatacc ttagctactg
     6601 ccatgtaaat aaaacacaaa aacctgcatt tatcattaac aataaattac aaaaacagta
     6661 taatgaccga gctgccatga gcgcataccg actcaacctg agccctttgt aacacacaaa
     6721 atatggatat atccctaggc aaaacaatat aacaagccaa acatcctaaa gataagccgg
     6781 caaggcaata cactctataa aactgtgccg agcaaaattt ttacaaagcc ctcaaccggt
     6841 atcgccgccc atatgccgca gcatccgtct tccactttat atccgcccgc aaaccatgac
     6901 cgccgctcct gatatcctct accggcaagc cgccgccctt ttggaacaat ccaataccgc
     6961 ccaagccctg cccctgttgc aacaggcggc agagcaaggt tatgcggaag ctgctttcgt
     7021 attgggcaac catctgctgc aaaacggcca accggagcag gcactttcat ggttggaagc
     7081 cgccgcggcc caacgccatc ccaaagcact cttctccctg ctgcaacaac gcgaacacaa
     7141 cggcaccccg accggacagc ttctcaacga ctatgcctgg ctgggtgagc aggggcactc
     7201 agaagcccaa ttaatcctca tgcgttacca cgcgcaacgc aacgatccac aatcgctcta
     7261 ctgggcggaa cttgctgccg cccgatatgc cgcacctgcg tattaccatc tggcacgcca
     7321 tcatcaacgc caaggcgacg ttgaaacagc catcgaacaa tacgaaaaag cggcagcact
     7381 cggcgtaact gccgcctgct ggcaacttgg tcaaatctac ttctacggta caggtgtcag
     7441 ccccaaccac gcacaagccg aacactatct cgaaccagcc gcacaagccg gccacatcgc
     7501 cgcacaaacg ctgctggctg accttcttgc cgcccaacgc aaacctgaag ccttggaatg
     7561 gtatcgtcgt gccgccgata aggaacaagc ggaagcacag tctaagctgg cccaatacgc
     7621 cctgaccggc gaactttccg aacgcgatcc gttccaagcg gcacgatatg ccaaagccgc
     7681 tgccgagaaa aaccatcctg aagccctgaa aatcatgggc gacctctacc gctacggtct
     7741 cggtatcaaa gccgacaacc atatcgcgca agattactac caccgtgccg ccgcgctggg
     7801 ttctgccgcc gcagcacaaa aactcatcag cgacgccgcg ctgtaccatc cgcaacaata
     7861 cgaacaaatc aaaactgccg ccctgcaaca acaacaaacc gaaaccatct accgtttggc
     7921 ggaagcacaa gcctgcgcca tcggccgtcc cgccgactac aatgccgcgc gaaaaaatta
     7981 catggaagct gccgggttcc accataaaaa cgcagcggca gccttaggcc gcatctacca
     8041 ttacggcctc ggtacggcgc aagatcctcg ggcggctgca cactggtacg ccattgctgc
     8101 cgaacaaaac cacccttccg cccaatacca cctcgcctgt ttttactatc acgggcaagg
     8161 tgtcggctgt catgttccga ccgcctgcta ctggctgcag gccgccatcg gcaacggcca
     8221 cacttcggcc gaatcattaa tatccctatt agaacaatgg cgacgcgaag cacaccatgc
     8281 catcggacaa aaggccgtct gaaaagattt acactcgcat tttttgacaa tctttaacta
     8341 ttcccctaat atttgccagt tatttttcac ggacacgcca ttgttttcat ttctttctga
     8401 aaacaccttg tccgcgcatc aataccatga cactcggcgg ataacgccaa gcgttgaaac
     8461 acactacatc cggaacaaaa acggatgctc ggaaaaatat ttctaggagg tgaaacaaca
     8521 tggaatggga attcaacagt tattacacac tgattgccgc cacgctcgtg ttgctggttg
     8581 gtaaatttct ggttcaaaaa atcaaattct tacgagactt caatattccc gagccggtcg
     8641 ctggcggttt ggttgccgcg attatcctgt ttgcgctgca cgaggcgtac ggcgtgagct
     8701 tcaaatttga gaaaccgctg caaaatgcgt ttatgctgat tttcttcacg tccatcggct
     8761 tgagcgcgga tttttcccgt ttgaaggcgg gcggtttgcc gctggtggtt tttaccgcga
     8821 ttgtgggcgg atttatcttg gtgcaaaact ttgtcggggt cggactggct acggctttgg
     8881 gtttggaccc gctcatcggt ctgattaccg gttcggtgtc gctgacgggc ggacacggta
     8941 cgtcaggtgc gtggggacct aattttgaaa cgcaatacgg cttggtcggc gcaaccggtt
     9001 tgggtattgc atcggctact ttcgggctgg tgttcggcgg cctgatcggc gggccggttg
     9061 cgcgccgcct gatcaacaaa atgggccgca aaccggttga aaacaaaaaa caggatcagg
     9121 acgacaacgc ggacgacgtg ttcgagcagg caaaacgcac ccgcctgatt acggcggaat
     9181 ctgccgttga aacgcttgcc atgtttgccg cgtgtttggc gtttgccgag attatggacg
     9241 gcttcgacaa agaatacctg ttcgacctgc ccaaattcgt gtggtgtctg tttggcggcg
     9301 tggtcatccg caacatcctc actgccgcat tcaaggtcaa tatgttcgac cgcgccatcg
     9361 atgtgttcgg caatgcttcg ctttcgcttt tcttggcaat ggcgttgctg aatttgaaac
     9421 tgtgggagct gaccggtttg gcggggcctg taaccgtgat tcttgccgta caaaccgtgg
     9481 tgatggtttt gtacgcgact tttgttacct atgtctttat ggggcgcgac tatgatgcgg
     9541 cagtattggc tgccggccat tgcggtttcg gcttgggtgc aacgccgacg gcggtggcaa
     9601 atatgcagtc cgtcacgcat actttcggcg cgtcgcataa ggcgtttttg attgtgccta
     9661 tggtcggcgc gttcttcgtc gatttgatta atgccgcgat tctcaccggt tttgtgaatt
     9721 tctttaaagg ctgattttcc gcctttccga caaagcacct gcaaggttta ccgcctgcag
     9781 gtgcttttgc tatgatagcc gctatcggtc tgcaccgttt ggaaggaaca tcatgtatcg
     9841 gaaactcatt gcgctgccgt ttgccctgct gcttgccgct tgcggcaggg aagaaccgcc
     9901 caaggcattg gaatgcgcca accccgccgt gctgcaaggc atacgcggca atattcagga
     9961 aacgctcacg caggaagcgc gttctttcgc gcgcgaagac ggcaggcagt ttgtcgatgc
    10021 cgacaaaatt atcgccgccg cctacggttt ggcgttttct ttggaacacg cttcggaaac
    10081 gcaggaaggc gggcgcacgt tctgtatcgc cgatttgaac attaccgtgc cgtctgaaac
    10141 gcttgccgat gccaaggcaa acagccccct gctgtacggg gaaaccgctt tgtcggatat
    10201 tgtgcggcag aagacgggcg gcaatgtcga gtttaaagac ggcgtattga cggcagccgt
    10261 ccgcttcctg cccgtcaaag acggtcagac ggcatttgtc gacaacacgg tcggtatggc
    10321 ggcgcaaacg ctgtctgtcg cgttgctgcc ttacggcgtg aagagcatcg tgatgataga
    10381 cggcaaggcg gtaaaaaaag aagacgcggt caggattttg agcggaaaag cccgtgaaga
    10441 agaaccgtcc aaacccacgc ccgaagacat tttggaacat aatgccgccg gaggggatgc
    10501 agacgtaccc caagccgcag aaggcgcgcc cgaaccggaa atcctgcatc ctgacgacgg
    10561 cgagcgtgcc gataccgtta ccgtatcacg gggcgaagtg gaagaggcgc gcgtacaaaa
    10621 ccagcgtgcg gaatccgaaa ttaccaaact ttggggagga ctcgataccg acgtgcaaaa
    10681 agagttggtc ggcgaacaac gcaagtgggc gcaggaaaaa atcagcaact gccgacaagc
    10741 cgccgcgcag gcagaccggc aggaatacgc cgaatacctc aagctgcaat gcgacacgcg
    10801 gatgacgcgc gaacggatac agtatcttcg cggctattcc atcgattagg ggcaaaccga
    10861 tgaataccgt cccaaaaagc aggattcccg tcaaaccgct gcccgaaaaa accacagacg
    10921 aagccaaagt cgaaaaatgg cggcagctcg gtgcggaaca cggtttgtcg ggcgaatggg
    10981 cagttgccgc cagattgggc gaaaacggtt ttaccgaaga acagatggaa aatatcgcca
    11041 acctgttcgg cagataaagg gaaaattgac ggaaatgccg tctgaaaccc tgttatcggt
    11101 ttcagacggc attttgacca atacggtacg caggcgcaaa acagccgtct tttcctgcgt
    11161 ttcctatgct gatgtttcaa cacacaagac ggcacataaa gcgttgccct atgtgccgtc
    11221 ctgattcgga aggggttacg cccctcccaa atatagtgga ttaacaaaaa ccagtacggc
    11281 gttgcctcgc cttagctcaa agagaacgat tctttagggt gctgaagcac caagtgaatc
    11341 ggttccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
    11401 actataaatc gagcctaaaa caatgccgtc tgaaacggaa atctgtttca gacggcattg
    11461 ttacattcaa acggcgggcc gtttatttga atttgtaggt gtattgcagg ccgatgatgt
    11521 cggcgtggtt tttgaaacgt gcggaagacg cgcctttgct gtccacatcg ttgccgcttg
    11581 ccttcgccgt gcggtaggtg gtgtcgttga tgtggatgtg ggtgtaggcg gcatcgacga
    11641 cgtggttttt gccgatatgg tatttcatac cggcggagaa ccagatgcgg ttgccgtcgg
    11701 gcaggctgtt catgcggtag tcggcgttgc ggacgggcga tttgtcaaaa gcgatgccgg
    11761 cgcgcagttg cagcggttcg ctgatttgat aagaaccgcc gaagccgact ttgtaggtgt
    11821 tgcgccagtt gggggtgatg gtggtgcggt cggatttgcc tttgacgacg gttttttctt
    11881 tttcaaaaac cagttccgcc ttatcgaagc ggctgtggcg cgtccaagtt acgtcgccga
    11941 acaggtcggc tttatcggac actttgtaca taccgtgtac ggacaaagac tcaggcgtaa
    12001 cgattttaac gcgggctttt tcattcgccg tgtagccgtt tgctgcaagc atcgtactcc
    12061 acattgcttt cgccgccgcg ccgtctgccg cccattcggc atcgcctttg agcgtgtgcg
    12121 agactttgga acggtagttc acgcccacgc gcgcgcggtc gttgatgtcc cacatccacg
    12181 ccagttggta gccgaagccc caatcgctgc ctttgacatc ggcgtgtccg tcggcctgaa
    12241 tttttgcagc ttcggctgca ccgttaggtt tgggcggttt tgccgtcaat atctctgctt
    12301 tactcttaat cccccagtcg gcatatttgc gcagctcggc ggaagtatgt tgggcgatga
    12361 tgcctgcgcc gaaggaatgg cgttcgttga gtttccacgc ggcgacaggt tcgacggcga
    12421 tgctggtcag accgagtttg ttgatgttgt ggcgcaacac ggaatctttt tcgtattcgg
    12481 tggcagaacc gaaggggacg tacacgccca agcctacggt cagattgtcg ttgactttgt
    12541 atgcgccgta gatgtggggc gcaaccgtgg ttttggtgat tttgccgttt ttcgaacctt
    12601 ggacgggaag cccggtaaag tcggtggcgg aatccgcctc ataatgaatg ctgggcagca
    12661 cgatgttggc gttgacggaa atctggctgc tgtcgagttt ggtcaggccg gcagggttgt
    12721 agaagatggt cgatgcgtcg gcggcttctg cggcggcggc atttgccgtg ctttgcgcgt
    12781 tgaccgactg tgtgccgaag tggtagccgg aggcttgtgc ggatgcggcg gcaaaggcag
    12841 tgccgagcag taggacggtt tttttcagtg cggaaggggt catttcggtt tccgtaaaaa
    12901 ggcggacggt ggataaatat agtggattaa caaaaatcag gacaaggcga cgaagccgca
    12961 gacagtacag atagtacggc aaggcaacgc cgtactggtt taaatttaat ccactataaa
    13021 aaaggcagtc ggaaatgcct tgtttcgctt tagtataggt actcgatttt atccgatgtt
    13081 gccggatttg cacaattttt tcagagtttg cccgaaccgc cgcgccgccg caaaaaatgc
    13141 cgtctgaagc ctcgggcatc gacttcagac ggcattttcc actcagggcg gattatttga
    13201 cgcgcagcac ttccagcgta ttggtcgaac cggattcgcg catttgcgag ccgctggtaa
    13261 tgatgtattg gtcgccggaa tgcaggatgt tgtgttccac cagcatggtt tcgacttcgt
    13321 ttaacgccgt gtcgtggtcg gtgctggttg ccaaaatcag cgggcgcacg ccccggtaca
    13381 tcgccatacg gcgttgggcg gaaacgctcg gggtcagcgc gaaaatcggc agggtgatgt
    13441 tgtggcggct gatttcaaag gcggtcgaac cgctttcggt cagggcgacg atggctttgg
    13501 cgtgaaccgc gcgcgccacg ctgaccgcac cgccggcaac cgccaggttg gtgctgaccg
    13561 cttcgggata ctcgacctgt tcggcaacgc cgttgagcga atcctgctct ttttccgcag
    13621 ccgcgcagat aatcgccatt tggctgacgg tttcaaacgg atacgcgccg acggcggttt
    13681 cggcggaaca catcaccgca tcggtaccgt ccaataccgc gtttgccaca tcgctgactt
    13741 ccgcgcgggt cggtacgggg ttggtaatca tcgattccat catttgcgtc gccgtaatgc
    13801 tgaagcggcg caactcgcgg gcgcggcgga tcatccgttt ttgcagggcg gggacggcgg
    13861 cgtgtccgac ttcgaccgcc aagtcgccgc gcgcaaccat aatgccgtcg ccggcgagga
    13921 tgatttcgtc caagttttca atcgcttcta cgcgttcgat tttggaaacc aaaccggggc
    13981 gcacggccgt gctgcctttc atttcttctt cgactttggc gcgcgcgata tgcaaatctt
    14041 cggcggattt cacaaagctg atggcgaggt agtcgcaacc gatggcaatc gcggttttca
    14101 ggtcgcggaa gtctttttcg gtcaacgcgc ctgcggacag accgccgccg cgtttgttga
    14161 tgcccttgtt gcttttcagg acgtggctgt tttccaccct tgtgataatc ctgctgcctt
    14221 cgacggattc cacggtcagg gtcagcaggc cgtcgtccag ccacaagaca tcgcctgcgg
    14281 caacgtcatc gggcaggtcg cggtagtcca aaccgaccgc ctcgcgcgtg ccttcgcctt
    14341 caagcgcggc atccagtagc agcgtttcgc ctttgttcaa ttcgatgccg ccgccggcga
    14401 ttttgcccac gcggattttc ggcccttgca ggtcggcaat gatggcgatt tcctgtccgg
    14461 cgcgttttgc cgcttcgcgc acgatgcggg cgttttcctg atggaattcg ggcgtgccgt
    14521 ggctgaagtt gaagcggacg acgttcaggc cgccgacgcg gatcatatct tccaacagtt
    14581 cgacgttgtt gctgcccggc ccaagggtgg cgacgatttt ggtgttgtgg ctgatgcggg
    14641 tcagatcgcg gcttgtctgg ttcatatgaa agtccttttg gtctcaatcg ggtgttttgc
    14701 ggtattttgt tacaaaatta cagaaatttg gaaccggttt gatgtccatt tgatgaacgc
    14761 ggcggaatat tctgtaaaaa tatgatttaa attaatagtt tgatatttta cctgcaaacc
    14821 gccttttttg gtgcaaaatt acacggtttt atgacttagg ctaaatttat ttttgggact
    14881 gt
//
LOCUS       DAXHTY010000047        14862 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXHTY010000047 DAXHTY010000000
VERSION     DAXHTY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14862
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.047"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..3973
                     /gene="iga"
                     /locus_tag="ACFNMK_001195"
     CDS             <1..3973
                     /gene="iga"
                     /locus_tag="ACFNMK_001195"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC6364471.1"
                     /translation="PDNLYFGFRGGRLDANGNDLTFEHIRNVDEGARIVNHNTGHAST
                     ITLTGKSLITAPQNLSVYEIRNDYDDDDYYGYYSYRKPIPQGKDLYYKNYRYYALKSG
                     GSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNNQRISGFSGFFGEENGKGHNG
                     ALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSGRPTPHARDFVNKSSARKDAH
                     FSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNVSNITANITATDNAKVNLGYKN
                     GDEVCVRSDYTGYVTCNTDNLSDKALNSFGATQINGNVNLSQNAALVLGKAALWGQIQ
                     GQGNSSVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNASDAQSANKYHTLKINHLSGN
                     GHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEPNQEGLNLFDASSVQDRSHLS
                     VSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVKPAPSPATNTASQAQKATQTD
                     GAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAEAERKSAKLAKQKAEAER
                     EARELATRQKAEQERSSAELARRHEKEREAAELSAKQKVEAEREAQALAVRRKAEAEE
                     AKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQSQPQRRKRRAAPQDYMAASQD
                     RPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELLAKQRAEAEREAQALAARRKA
                     EAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQALAARQQKALARQQEEARKAAE
                     LAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAELARRQEAERQAAELSAKQKAE
                     TDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQDYMAASQNRPKRRGRRSTLPAP
                     PSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDENVSESVDTSDKQPQDNTELHE
                     KYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRAQAAA
                     QADAVSTNTNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRD
                     YASAQYRRFSSKRTQTQIGIDRSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLY
                     GKYYLNDAWYVAGDIGAGSLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSA
                     GIRYSRLSSADYKLGNDSVKVSSMSVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANY
                     GKGGVNVGGNSFAYKADNQQQYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQ
                     IRF"
     gene            complement(4151..5353)
                     /gene="trpB"
                     /locus_tag="ACFNMK_001196"
     CDS             complement(4151..5353)
                     /gene="trpB"
                     /locus_tag="ACFNMK_001196"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC6364472.1"
                     /translation="MKNYHAPDEKGVFGEHGGLYVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTAAK
                     DEEALEAFDLLCKYEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(5538..6227)
                     /locus_tag="ACFNMK_001197"
     CDS             complement(5538..6227)
                     /locus_tag="ACFNMK_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6364473.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAQMPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            6439..7218
                     /gene="rsmA"
                     /locus_tag="ACFNMK_001198"
     CDS             6439..7218
                     /gene="rsmA"
                     /locus_tag="ACFNMK_001198"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC6364474.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            7391..8119
                     /locus_tag="ACFNMK_001199"
     CDS             7391..8119
                     /locus_tag="ACFNMK_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6364475.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            8192..8551
                     /locus_tag="ACFNMK_001200"
     CDS             8192..8551
                     /locus_tag="ACFNMK_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364476.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            8556..9002
                     /locus_tag="ACFNMK_001201"
     CDS             8556..9002
                     /locus_tag="ACFNMK_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC6364477.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCIMPDMTDPESYRRLKVWVLWRQPKKTAETDTPS"
     gene            9034..10308
                     /gene="folC"
                     /locus_tag="ACFNMK_001202"
     CDS             9034..10308
                     /gene="folC"
                     /locus_tag="ACFNMK_001202"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC6364478.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DFDHQAFLGDTVEQVGFEKAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHGIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            10321..11319
                     /gene="ftsN"
                     /locus_tag="ACFNMK_001203"
     CDS             10321..11319
                     /gene="ftsN"
                     /locus_tag="ACFNMK_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6364479.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETNGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            11312..11809
                     /locus_tag="ACFNMK_001204"
     CDS             11312..11809
                     /locus_tag="ACFNMK_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC6364480.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVAFFFA
                     KLFAAPFADIAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            12017..13561
                     /gene="purF"
                     /locus_tag="ACFNMK_001205"
     CDS             12017..13561
                     /gene="purF"
                     /locus_tag="ACFNMK_001205"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC6364481.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAQVHRLVRGAYGVIAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            13661..14152
                     /gene="greB"
                     /locus_tag="ACFNMK_001206"
     CDS             13661..14152
                     /gene="greB"
                     /locus_tag="ACFNMK_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC6364482.1"
                     /translation="MSTETKNYITPAGWQALKNELYRLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVIDPELREATDQVFFSATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            14211..14837
                     /locus_tag="ACFNMK_001207"
     CDS             14211..14837
                     /locus_tag="ACFNMK_001207"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC6364483.1"
                     /translation="MRKIRTKICGITTLEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT         4049 a         4139 c         3716 g         2958 t
ORIGIN      
        1 tcccgataac ctatatttcg gtttccgtgg cggtcgtttg gatgccaatg gcaatgactt
       61 gacttttgaa cacatccgca acgtggatga aggcgcgcgc attgtcaacc acaacacagg
      121 ccacgcctcc acaatcacat taaccggcaa atcattaatt accgctcctc aaaatctttc
      181 tgtttatgaa atccgaaatg attacgatga tgacgattat tatggctatt acagctatag
      241 aaaacccatt ccgcaaggca aagatcttta ttacaaaaac tatcgttatt acgccctaaa
      301 atccggcggc agcgtgaacg caccgatgcc cgaaaacgga caaacggaaa ataacgactg
      361 gattttaatg ggcagcacac aagaagaagc caagaaaaac gcgatgaacc acaaaaacaa
      421 tcagcgtatt agcggtttta gcggtttctt tggcgaagaa aacggaaaag gacataacgg
      481 cgcattaaac cttaatttca acggcaaaag cgcgcaaaac cgtttcttgt taacaggtgg
      541 cacgaattta aacggaaaaa taagcgtaac tcaaggcaat gtcttgttat caggtcgtcc
      601 aacaccacac gcaagagatt ttgtgaacaa atcttcagcc cgaaaagacg cacatttctc
      661 caaaaacaat gaagtcgtat ttgaagacga ctggataaac cgcacattca aagccacaga
      721 aattgcggtt aatcaatccg catcattctc ttccggcaga aatgtatcca acattaccgc
      781 caacatcacc gcaacagaca acgccaaagt aaatttgggt tacaaaaacg gcgatgaagt
      841 ttgcgtgcgc tcggactata ccggttacgt tacctgcaac acagacaact tatccgataa
      901 ggctttaaac agctttggag cgacacagat taacggcaat gtgaatttga gtcagaatgc
      961 ggcattggtc ttaggcaagg ctgcattatg ggggcaaatt caagggcaag gaaacagcag
     1021 cgtcagccta aaccaacata gcaaatggca tttgaccggc gacagccaag tacacaatct
     1081 gtcattggcg gatagccata ttcatttgaa caacgcttcc gacgcgcaaa gtgcaaataa
     1141 ataccacacg ctcaaaatca atcatttatc cggtaacggg cattttcatt atctgacgca
     1201 cctggcgaaa aatcttgggg ataaagtgct tgtgaaggaa tccgcatccg gtcattatca
     1261 gctccatgtt caagataaaa caggcgaacc caatcaggaa gggctgaatc tctttgatgc
     1321 atcatccgta caagaccgtt cgcacctttc tgtttccttg gcgaataacc atgtagattt
     1381 aggcgcattg cgttatacaa tcaaaacaga aaacggtatt acccgattgt acaatcctta
     1441 tgccgagaac cgccgccgag tcaaaccggc cccgtctcct gccacaaaca cggcttctca
     1501 agcacaaaag gcaacacaaa cggacggtgc acaaattgcc aagcctcaaa atatcgtcgt
     1561 cgcaccacct agcccgcagg caaatcaagc cgaagaagca aaacgccagc aagcaaaagc
     1621 ggagcaagtg aagcgtcagc aagcagaagc agagcgtaaa tcggcaaagt tagcaaaaca
     1681 aaaagcagaa gccgaacgcg aagcaagaga gctggcgacc cgccaaaaag cagaacaaga
     1741 acgcagcagc gccgaacttg cacgtcgaca tgaaaaagaa cgcgaagcgg cagagttgtc
     1801 ggcaaaacaa aaagtcgaag ccgaacgtga ggctcaagca ttggcggtac gccgaaaagc
     1861 agaagccgaa gaggcaaaac gccaagctgc agagcttgca cgccggcatg aaaaagaacg
     1921 cgaagcggca gagttgtcgg caaaacaaag ggtcggggaa gaggaacgcc gtcaaacagc
     1981 ccaatcccag ccgcaacgcc gcaaacgccg tgccgcaccg caggattata tggcagcttc
     2041 ccaagaccgt cctaaacgcc gcggacaccg ttcggtacag caaaacaatg tggaaattgc
     2101 ccaagcccaa gcagaacttg cgcgccgaca gcaggaagaa cgcaaagcgg cagagttgtt
     2161 ggcaaaacaa agggcagaag ccgaacgtga ggctcaagca ttggctgcgc gccgaaaagc
     2221 agaagccgaa gaggcaaaac gccaagctgc cgaacttgca catcgccagg aagcagaacg
     2281 caaagcggca gaattgtcgg caaaccaaaa agccgcagct gaagcccaag cattggcggc
     2341 acgccaacaa aaagcccttg cgcgtcaaca ggaagaagca cgaaaagctg cagagttagc
     2401 cgtcaaacaa aaagcggaaa ccgaacgcaa aaccgccgaa cttgccaagc aaagggcagc
     2461 agccgaagca gcaaaacgcc agcaagaagc tcgccagacc gccgaacttg cacgtcgtca
     2521 agaagcggaa cgccaagcag cagagttgtc ggcaaaacaa aaagccgaaa cagatcgaga
     2581 agcggcagaa tcggcgaaac gaaaagctga ggaagaagag caccgtcaag cagcccaatc
     2641 ccagccgcaa cgccgaaaac gccgtgccgc acctcaggat tatatggcag cttcccaaaa
     2701 tcgtccgaaa cgtcgcggac gcagatctac tctgccggca ccgccctcgc catcatttga
     2761 ttcgtcagct tacgcagcac ccagggcctt gcataatccg gactggtatg aaaatgacta
     2821 tgaagaaatc cccttggatg cgttggaaga tgaaaatgta tctgaatcag ttgacacatc
     2881 agacaaacag cctcaagaca atacggaact tcatgaaaaa tatgaaaatg attatgaaga
     2941 aatccccttg gatgcgttgg aagatgaaga tgtatccgaa tcggttgaca catcagacaa
     3001 acagcctcaa gacaatacgg aacttcatga aaaagttgag acagtgagtt tgcaaccaag
     3061 agccgcgcag ccgcgagccc aagccgccgc gcaagccgat gcagtcagca ccaataccaa
     3121 ctcggcttta tctgacgcaa tggcaagcac gcaatctatc ttgttggata caggtgcttc
     3181 attaacacgg cacattgcac aaaaatcacg tgctgatgcc gaaaaaaaca gcgtttggat
     3241 gtcgaacatc ggttatggcc gtgattatgc ttccgcacaa tatcgccggt ttagttcgaa
     3301 acgcacgcaa acacaaatcg gcattgaccg cagcttgtcc gaaaatatgc agataggcgg
     3361 cgtattgact tactctgaca gtcagcatac ttttgatcag gcgagcggca aaaatacttt
     3421 tgtgcaagcc aacctttatg gtaagtatta tttaaatgat gcttggtatg tggccggcga
     3481 tattggtgca ggcagcttga gaagccggtt acaaacgcag caaaaagcaa actttaaccg
     3541 aacaagcatc caaaccggcc ttactttggg caatacgctg aaaatcaatc aattcgagat
     3601 tgtccccagt gcgggaatcc gttacagccg cctgtcatct gcagattaca agttgggtaa
     3661 cgacagtgtt aaagtaagtt ctatgtcagt gaaaacactg acggccggac tggattttgc
     3721 ttatcggttt aaagtcggca accttaccgt aaaacccttg ttatctgcag cttactttgc
     3781 caattatggc aaaggcggcg tgaatgtggg cggtaattcc ttcgcctata aagcagataa
     3841 tcaacagcaa tattcagcag gtgccgcgtt actgtaccgt aatgttacat taaacgtaaa
     3901 tggcagtatt acaaaaggca aacaattgga aaaacaaaaa tccggacaaa ttaaaataca
     3961 gattcgtttc taaaatacta aattcatagc aaaataaaat gccgtctgaa ctcaggcttc
     4021 agacggcatt tttatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag
     4081 tacagataga cggcaaggcg aggcaacgcc gtactggttt ttgttaatcc actatatcag
     4141 acggacgagg ttacagttta atccctttga gcttcgcgac ggtattgatg tctttgtcgc
     4201 cacgacctga gaggttgacc agaatcactt ggtctttacc cattttcggc gcatttttca
     4261 ccgcccaagc aacggcgtgg ctggattcga gcgcggggat gatgccctca tatttgcaca
     4321 acaaatcaaa ggcttcgagt gcttcttcgt ctttggcggc ggtgtattcg acgcgcttga
     4381 tgtcgtgcag atggctgtgt tccgggccga tgccggggta atccaagcct gcggaaacag
     4441 agtgcgtacc caaaacctga ccgttttcgt cctgcatcag atagctgcgg aaaccgtgca
     4501 atacgccaat cggtgcgccc gaagtaatcg gcgcggcgtg atcgggggtg ttcacgccca
     4561 aaccgccagc ctccacgccg acgaggcgca cgttttcttc gccgatatac gggtggaaca
     4621 aaccgatggc gttcgatccg ccgcccacgc aggcaacggc aacgtcgggc tgtctgccga
     4681 tggcttccaa catttgttct ttggcttcat tgccgatcac gcattgaaag tcgcgcacca
     4741 tttcgggata cggcgcggga ccggcggcgg taccgatgat gtagaatgtg tcatccacgc
     4801 gggcgaccca ttcgcgcatg gcttcgttca tcgcgtcttt caaagtacgg ctgccgctgt
     4861 caacgccgac gacattcgcg cccaataatt tcatacggaa cacgttgggc atttggcgtt
     4921 ggatgtcgtc cgcacccatg tacacgtcgc aagtcatgcc gaagcgggcg gcaacagtgg
     4981 cactcgccac gccgtgctga cccgcgccgg tttcggcgat gacgcgtttt ttacccatac
     5041 gttttgccaa cagtgcctga ccgatggtgt tgttgacttt gtgcgcgccg gtgtggttca
     5101 agtcttcgcg cttcaaccaa atttgcgcgc cacccaaatg ctcggacaat cgcgcggcat
     5161 ggtaaacggg gctgggcctg ccgacgtagt gtttcaaatc gcggtggaat tcttcccaaa
     5221 acgacgggtc gtttttcgct gccttatagg catccgccag ctcttgcaag gcgggaatca
     5281 gggtctcgga aacataaagc ccaccgtgtt cgccgaaaac gcccttctcg tcgggtgcgt
     5341 ggtaattctt catcagattt cgtccttgtg cttcaaattt tcagacggca atttatagtg
     5401 gattaacaaa aaccggtacg gcgttgcccc gccttgccgt actatttgta ctgtctgcgg
     5461 cttcgccgcc ttgtcctgat ttttgttaat ccactatatt tttcaaacat gccgtccgaa
     5521 cgggaaattg tcggcaatta taactgcttt tcacacaagc ccgccatttc aaaaaacgcc
     5581 cgatacgccg ccgctttttt cgccaaactc aaaggatagg cacgcgaagt cgaaggcagg
     5641 cgcgtcagcg tcaaatcccg tccggcaaac ggaaacggca cggtttcgcc cgttttcggc
     5701 attttgatgc cgccaccctg aatatcgagc aggatttccg tcgccttgcc gcctgtcgta
     5761 caaatatggc ggcactcggg catctgcgcc aacaccgccg ccaaatcaac ggtttcaact
     5821 acctttaaaa acttgtcgga cgcattgccg tgctgacgta ccgctttcaa cacggtcggg
     5881 caggacgcaa tcccccgttc gtgcaaaaac gccttgattt tctcggcatc aaacgctttt
     5941 tcagacaacc tttggaaatg cgccgcatca ttaaaaaaca ccagcccgta aacgcgccac
     6001 atatcgtttt ggaaattcgg ataatgaaac tgcatcgcgc gtttgtcttc cttgggcgga
     6061 aacgtcccca tcatcatgac cgcagccttc ggcggcaaca cggaatcgaa gggatgggtt
     6121 tcgatttcaa gcagattttc agacacgttc aatcctccaa caggcaaaac ggcagacagc
     6181 cggtttttgc accaaaccgc caaacacggc aagccttcaa acagcattat cacgccctgc
     6241 cgttttcggt tacaatgccg acatcaaacg gatactgtaa acacatttat gtttcaacac
     6301 acaggacgac acttaaagca ccgccctatg tgccatcctg atttggaagg ggttacgccc
     6361 ctcccaaata gagtctgatt ctaccgcccc gaaggacaga tgtccaagtg gcggggtttc
     6421 aaccgaaaag gaaacacgat gaaagaacac aaagcccgca agcgtttcgg gcagaatttt
     6481 ttgcaggaca cgcggatcat cagcgatatt gtcaacgccg tgcgcccgca ggcggatgat
     6541 gtcgtgattg aaatcggtcc gggtttggcg gcgattaccg aacctttggc gaaaaagctg
     6601 aaccgcctgc acgttgtcga aatcgaccgc gacatcgtat gccgtctgaa aacgctgccg
     6661 tttgcggaca agctcgtcat tcatgaaggc gacgtgttgc agtttgattt caacggcatc
     6721 gcaggcaaaa agaaaatcgt cggcaacctg ccgtacaaca tttccacgcc gcttttgttc
     6781 aagctggcgg aggtggcgga cgatgtcgtc gatatgcact ttatgctgca aaaagaagtg
     6841 gtcgatcgta tggttgccgc gccgaaaagc aacgactacg gccgcttggg cgtgatgctg
     6901 caatatttct tcgatatgga aatgctgatt gacgtgccgc ccgaatcgtt cgaccccgcg
     6961 ccgaaagtgg attcggccgt cgtccgcatg attccggtga aacaccgtat cggcaaggcg
     7021 gacgatttcg agcatttcgc caaactcgtg aaactcgcct tccaccaacg ccgcaaaacc
     7081 atacgcaaca atctgaaaga gcttgcaggc gacgatgatt tgcaggcagt cggcatcaat
     7141 ccgcaagacc gcgccgaaca tatcgcgccg gagaaatatg tggcgttgag caattatctg
     7201 gcgggcaagg ccgtctgaaa agggagtaag ccgatgacga aatacctgct tttacccctg
     7261 atactgccgc ttgcagcctg cctgaccaat ccgaacgacc gtttttttga cggcagacgc
     7321 tacacgcttc atcatatgga gctcccgtaa cgacaacctg caacggcaaa tgccgtctga
     7381 aagaaaagac atgatcaaat tcaaaaacgt acacaaacat ttcaaagacc tgcacgtcat
     7441 caacggcgtg aacttggaaa tcaaaaaagg cgaagtggtc gtcgtctgcg gaccttcggg
     7501 cagcggcaaa tccacactca tccgcaccgt caaccagctt gaaagcatcg aaagcggcga
     7561 gatttgggtg gacggtgtca acgttgccga cccaaaaacc gatttgaaca aaatccgcga
     7621 agaagtcggc tttgtgtttc aaagtttcaa cctctacccc cacctgaccg tattggaaaa
     7681 catcaccctc gcaccgatga aggttaaagg tcaaaatgcg gaacaggcgg agaaaaaagc
     7741 gatggaactt ttggaacgcg tcggactggc gcacaaaaaa gacgcttttc cgtcccaact
     7801 ttccggcggc cagcagcagc gcgtggcgat tgcacgcggt ttggcgatgg aaccgcgcgt
     7861 aatgttgttt gacgaaccga cctccgcact cgaccccgaa atggtcggcg aagtgttgaa
     7921 agtgatgaaa gacttggcag aaagcggcat gaccatgatg tgcgtaaccc acgaaatggg
     7981 gtttgcccgc gaagttgccg accgcgtgat tttcgtcgat aaagggcaaa tcctcgaaga
     8041 cgaaacgccc gaagcgtttt tcaccaaccc gaaacacgaa cgcgccaagc agttcctgca
     8101 acaggtcatg acccactgat gctataccgt ctgaaaaccg aacgttcaga cggcatagcc
     8161 cgacaccgca agatgattga gaaagaaaac catgtatttt gttgaccgca ccgccgtcgt
     8221 cctcaagccg accgtccgct ttttggaatg gctcaaaagc accgacgaaa atatgcccga
     8281 cctgaccata gagcagcttc gtgccaactg ctccgtcttc ctcgttccgc agttcgacga
     8341 accggaagcc gtcgtttcct atttcgacga acgctaccgc cagatttttg aagccgaact
     8401 cgccggctgg gacatcgaca aggataaatg gccgcaagat atggggctga aggcgttttg
     8461 ggaatttttc gacatcgaaa tccacgatat ggtgttggat atggaagaag cggatttgaa
     8521 catcacgccc gtgttcgaca atatgatgta acgccgtgcg cgcctttcag acagcattga
     8581 ggccgtcccg catcctgaac atcctgaccg tatcgctcca ctcggcttcc cttgccgtct
     8641 gcctgacctg gttttacggc cggatgatgt ggttcggttt ggcggctttg gcggcaagtt
     8701 acgcatactc gctcaggata acgaatctga aacaccgcca tgccataacc gccattacca
     8761 tagaccgcga cggacaggcg gaaatcgtat ccggcaaaga aaaaacagca aaggcggcgg
     8821 cactgtcggg cagcagtatg gttacgcctt atgccttatt cttacaatgg gacaccggcg
     8881 gcaaaaccgt ccgccactgc atcatgcccg acatgacgga cccggaatcc taccggcggc
     8941 tgaaagtctg ggtgctttgg cggcagccga aaaaaaccgc cgaaaccgat acaccaagct
     9001 gatttcagac ggcatttgaa cacggaacac tcaatgaaaa cactgcaaga ctggctctcc
     9061 catttggaaa ccgcccacag cggcggtttg atcgatatgg gcttggaacg cgtaggcgaa
     9121 gtgaaaaaac ggatgaagct cacgccgcaa tgccccgtcg tcgttgtcgc gggaactaac
     9181 ggcaaaggtt cggtctgcgc ctacctgaca cagatttaca aacaggcggg ctacaaaacc
     9241 ggcacattaa ccagccccca tttattgcgt tacaacgaac gcatcgccat caacgccgaa
     9301 cccgtttcgg acgatacgat tattgcctct ttcgagcgca tcgaagcggc gcgcggcgaa
     9361 atatcgctga cttattttga attcaatacg ttggcggccg tcgacatctt catgcgcgaa
     9421 caggttgacg tgatgatatt ggaagtcggc ttgggcggac gcttggacgc ggtcaacgca
     9481 tttgacggcg attgcgcggt ggttactagt gtagattttg accatcaggc gtttttgggc
     9541 gatacggtcg agcaggtcgg ctttgaaaaa gcaggcgtgt tccgcggcgg caaacccgca
     9601 atctgcggtc aaaatcccgc gcccgcatcg ttgctttcac acgccgaagc cataggcgcg
     9661 aaactgctga tggtgcagcg cgatttcgag tttcacgcgc tggaaaacat ccaatggaac
     9721 taccgcttcc gaccgcagca ttcagacagc cccgctcgca accgtaattc cctgcccttc
     9781 cccgcattgc gcggcgcata ccaactttcc aacgccgcct gcgcgctgac cgtgttggaa
     9841 tgtttgaacg acaaattgcc ggtggacatc ggtgcaatca aacgcggatt gctgctggtt
     9901 gaaaatcccg gacgcttcca agtcctgccc ggccggccgc tgaccgtttt ggacgtcgga
     9961 cacaatcccc acgctgcccg cgccctgcgc cgcagcctga ttaatttggc gtttgcgcaa
    10021 aaacgcaccg ccgtattcag tatgctgtcc gacaaagaca tagacggcgt gttggaaatc
    10081 gttaaagacc agtttgacga gtggtatatc gcaccgttgg acgtaccgcg cggcatgacg
    10141 ctcgagagcc tgcaaaacaa actgcacgaa cacggcatcg aaaacataca gacattcgcc
    10201 gccgtccgcg aagcataccg ggcggcagcc tcaaaagcgg gcgagaacga cagaatcgtc
    10261 gttttcggct catttcatac cgttgccgat gtcatgtcgg tactttaaaa ggaaacttct
    10321 atgtccgaaa acaaacaaaa cgaagtcctg agcggttacg aacaactcaa acggcgcaac
    10381 cgccgccgcc tcgtaacggc aagctgcctg gttgccgcct cctgcatcct gctggcagcc
    10441 gccctcagtt ccggccctgc cgaacagact gccggcgaaa caaacggcgt agaaaacaaa
    10501 gcggcaggtg cggcacaaac ccctgccttg aaatccgccg ccgacaaacc gcaggacttg
    10561 gcaggcgaag acaagccttc tgccgccgac agcgaaatca gcgagcctga aaacgtaggc
    10621 gcgccgctgg tgctgattaa cgaccgcctc gaagacagca acatcaaagg tttggaagca
    10681 tccgagaaac tgcaacaggc agaaaccgcc aaaaccgcac cgaagcaggc aaaacaacgc
    10741 gctgccgaaa aagtgccggc aactgccgac agtacggata cggtagcggt tgaaaaaccg
    10801 aaacgcactg ccgaaacaaa accgcaaaaa gcggaacgca ctgccaaagc caagcccaaa
    10861 gccaaagaaa ccaaaaccgc cgaaaaagtt gccgacaaac cgaaaactgc cgccgaaaaa
    10921 accaaaccgg atacggcaaa atccgacagc gcggtaaaag aagcgaaaaa agccgacaag
    10981 gctgaaagca aaaaaacagc cgaaaaagac cgttcggacg gcaaaaaaca cgaaacggca
    11041 caaaaaaccg acaaagcgga caagaccaaa accgccgaga aggaaaaatc cggtaaaaaa
    11101 gccgccattc aggcaggtta tgccgaaaaa gaacgcgcct taagcctcca gcgcaaaatg
    11161 aaagcggcgg gtatcgattc gaccatcacc gaaattatga ccgacaacgg caaagtttac
    11221 cgcgtcaaat caagcaacta taaaaacgca agggatgccg aacgcgattt gaacaaattg
    11281 cgcgtacacg gtatcgccgg tcaggtaacg aatgaataac ctccccgttg ccgacctcct
    11341 cgtcttcgcc atcatcgccg cctgcatcgt gctatccgcg atgcgcggcg tgattgcgga
    11401 ggctggctca atggcggcat gggtggttgc cttttttttc gccaaactct ttgccgcacc
    11461 cttcgccgac atcgcctttg catcgttcca accccgcctg tttgcattgg ctctgtcgtt
    11521 catttcccta ttcgtcattg cctgtctgat ccagaaaata ctccgctcgc tgctgaccgg
    11581 ggcagtttcg gcggtcggtt tgggctttgc caaccgcatt ttgggcggcg tattcggtgc
    11641 attgaaaggc attttgatta ttaccctgct ggtcatgctc gcttcaaaaa ccgacctgcc
    11701 cgataccgaa gaatggcggc aatcttacac actgccgttt ttcgtatcgc tttccgaagc
    11761 cgtgttgaac catagcggcg gcacggcgga aactccggaa gacgattgat tgccatacgg
    11821 atatttcaga cggcattctc ttttatgccg tctgaaatgt tttttccttt cctcagccat
    11881 atcacagatt aaatcttatc ctccataaac ccacctatct gtccttcgct caaatattgt
    11941 caacaattca ggcaaaaaac caccgtttac tttataattc cgttttaccc ttaaacctcg
    12001 aatcggagaa gacgatatgt gcggcgtatt aggtttggtc agtcatgaac ccgtgaacca
    12061 gcttctgtac gacggcttgc agatgttgca gcacaggggg caggatgcgg cgggcattgc
    12121 aacggcggaa ggcggtacct tccatatgca caaaggcaaa ggcatggtgc gcgaagtgtt
    12181 ccgcacacgc aatatgcgcg atttgaccgg caacgccggc atcgcccacg tccgttatcc
    12241 tactgcgggc aacgcaggca gcagcgcgga ggcgcagcct ttctacgtca gctcgccgtt
    12301 cggcatcgtt ttggcgcaca acggcaacct caccaacact gccgaactgt atgaaaacgt
    12361 gtgtaacaaa cacctgcgcc acgtcaacac cagctccgat tccgaagtct tgctcaacgt
    12421 attcgcgcac gaattacgcc gcgaagtctc taaaaacgcc gacccgcacc ggctcaatgc
    12481 cgacaatatt ttcaacgccg ttgcccaagt ccaccgcctg gtgcgcggcg catacggtgt
    12541 gattgccatg attgcgggct acggtatgct cgccttccgc gacccttacg gcatccgccc
    12601 gctggtattg ggttcgcaaa ccgacagcga aggcagaaaa tcctatgccg tcgcctccga
    12661 atccgtcgcc ttcaacgcgc ttacctacga tttggaacgc gacatccggc cgggcgaagc
    12721 ggtatttgtc ggctttgacg gcacaatgat tgcccgccaa tgcagcgacc gtgccaaact
    12781 cagcccctgc ctttttgaat atgtttattt tgcccgcccc gactctgtga ttgacggcgt
    12841 atccgtttac caatcgcgct tggatatggg cgtgtcgctg gcagaaaaaa tcaaacgcga
    12901 gctgcccgtg gacggcatcg acgttgtgat gcccatcccc gacaccagcc gccccagcgc
    12961 gatggagctt gccgtccatc tcgacaaacc ctaccgcgag ggtttgatta aaaaccgcta
    13021 tatcggccgc acctttatta tgcccggaca ggcgacgcgc aaaaaatccg tgcgccaaaa
    13081 actcagcccg atggaaaccg aatttgcagg caaaagcgtg ttgctggtgg acgactccat
    13141 cgtgcgcggg acgaccagcc gcgaaatcgt cgaaatggta cgcgcggcgg gcgcgcgcaa
    13201 agtctatatc gcctccgccg cgcccgaagt gcgctatccc aatgtgtacg gcattgatat
    13261 gcccacgcgc gaagagttga ttgccaacgg gcgcagcgcg gcggaaatcg ccgccgaaat
    13321 cggcgcggac ggcatcgtat ttcaagactt gggcgatttg gaagccgtcg tcaaagcact
    13381 caatccgaaa atcgaatcct tcgattcgtc ctgtttcaac ggcatttatc agacaggcga
    13441 catcgatgat gcctacctcg accgcctgtc tgccgagaaa tccggctgcg gcggcctgaa
    13501 aatccacccg agtaggatgg aacacagcat cagcatcagc gatgcgggcg acgaagaata
    13561 aggcttccca aaccgcgatg ccgtctgaaa tccgaacgcc gggttttcag acggcatttc
    13621 ccatatcggc atcacatttc ccatttcatc cgcacgcaat atgagtaccg aaaccaaaaa
    13681 ctacattaca cccgccggct ggcaggcatt gaaaaacgaa ctttaccggc tggtcaacaa
    13741 agaacgcccc gaaatcgtcc aaatcgtcaa ttgggcggca ggcaacggcg accgcagcga
    13801 aaacggcgac tatctttacg gcaaacgccg tatgcgcgaa atcgaccgcc gcatccgttt
    13861 cctgaccaag cgtttggagg ctgccgtcgt catcgatccc gaattgcgcg aagcgaccga
    13921 ccaagtgttt ttcagcgcga ccgtcggcct gttgcgcggc gacgggcgcg agcaaaccgt
    13981 caaaattgtc ggcatcgatg aaatcgatac cgcgcaaaac aaaatttctt ggatttcgcc
    14041 gttggcgcgt tgcctgatta aagcgcgcga aggcgacgaa gtggttttga acacgccgga
    14101 agggcgtgag gaaattgaaa tcctttctgt cgaatacatc aaaatcgact gatcgggaat
    14161 gccgtctgaa acggcacaaa cgcatcgttt cccaaataaa ggaataccga atgaggaaaa
    14221 tccgcaccaa aatctgcggc atcaccacac tggaagacgc agcggccgcc gcagcggcag
    14281 gtgcggatgc cgtcgggctg gtctttttcc aaggcagcag ccgggccgtc gatattgccc
    14341 gcgcaaaaaa aatcaccgcc gcactgccgc cgtttgtcag cgttgtcgcc cttttcgtca
    14401 acgaaagcgc gcaaaacatc cgccgtatcc ttgccgaagt accgatacac atcatccaat
    14461 tccacggcga cgaagacgac gcattctgcc gccagttcca ccgcccctat atcaaggcca
    14521 ttcgtgttca gacggcatca gacatccgaa acgccgccga ccgcttcccc gacgctcagg
    14581 cactgctgtt cgatgcctac cacccttcgg aatacggcgg caccggacac cgcttcgact
    14641 ggacgctgct ggcggaatat tcgggcaaac cgtgggtact tgccggcggg ctgacccctg
    14701 aaaacgtcgg cgaagccgtc cgcatcaccg gagcggaagc ggtcgatgta tccggcggcg
    14761 tggaagcgtc taaaggcaaa aaagacccag ccaaagtcgc cgcctttatc gcaaccgcca
    14821 accgcctatc ccgttaaagc aacaaaaatt gccgccggaa tg
//
LOCUS       DAXHTY010000048        14856 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXHTY010000048 DAXHTY010000000
VERSION     DAXHTY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14856
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.048"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     regulatory      77..121
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            125..598
                     /gene="queF"
                     /locus_tag="ACFNMK_001208"
     CDS             125..598
                     /gene="queF"
                     /locus_tag="ACFNMK_001208"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC6364484.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            673..1359
                     /locus_tag="ACFNMK_001209"
     CDS             673..1359
                     /locus_tag="ACFNMK_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC6364485.1"
                     /translation="MYAFTDAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIALVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(1708..3000)
                     /locus_tag="ACFNMK_001210"
     CDS             complement(1708..3000)
                     /locus_tag="ACFNMK_001210"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC6364486.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            3078..4046
                     /locus_tag="ACFNMK_001211"
     CDS             3078..4046
                     /locus_tag="ACFNMK_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364487.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLHAFNKILQYGSPYRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGLHQANVLTAEYLNVGTDEAER
                     LCRDLSEFYADTPWHFTPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGKDSL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYASVLQQWEEHWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(4247..5713)
                     /locus_tag="ACFNMK_001212"
     CDS             complement(4247..5713)
                     /locus_tag="ACFNMK_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6364488.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQTDGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFDNRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(5955..8054)
                     /locus_tag="ACFNMK_001213"
                     /pseudo
     CDS             complement(5955..8054)
                     /locus_tag="ACFNMK_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(9005..9250)
                     /locus_tag="ACFNMK_001214"
     CDS             complement(9005..9250)
                     /locus_tag="ACFNMK_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364489.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            9362..9790
                     /locus_tag="ACFNMK_001215"
     CDS             9362..9790
                     /locus_tag="ACFNMK_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC6364490.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            9802..10278
                     /locus_tag="ACFNMK_001216"
     CDS             9802..10278
                     /locus_tag="ACFNMK_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC6364491.1"
                     /translation="MPIEIERRFLIENDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISELSRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(10628..11116)
                     /locus_tag="ACFNMK_001217"
     CDS             complement(10628..11116)
                     /locus_tag="ACFNMK_001217"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC6364492.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(11184..12239)
                     /gene="aroG"
                     /locus_tag="ACFNMK_001218"
     CDS             complement(11184..12239)
                     /gene="aroG"
                     /locus_tag="ACFNMK_001218"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC6364493.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            12900..13157
                     /locus_tag="ACFNMK_001219"
     CDS             12900..13157
                     /locus_tag="ACFNMK_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364494.1"
                     /translation="MTTQMGASRDVSKSAKGWSVGIGLNVGKQLTDSVDLEFDPYYRH
                     KTICKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK"
     gene            complement(13139..13360)
                     /locus_tag="ACFNMK_001220"
                     /pseudo
     CDS             complement(13139..13360)
                     /locus_tag="ACFNMK_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13513..14166)
                     /locus_tag="ACFNMK_001221"
     CDS             complement(13513..14166)
                     /locus_tag="ACFNMK_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6364495.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEQDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            complement(14286..14744)
                     /locus_tag="ACFNMK_001222"
     CDS             complement(14286..14744)
                     /locus_tag="ACFNMK_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364496.1"
                     /translation="MSSEHVFGRRFVGGYRSGTSEKGFQAVFGRVVTVENVIRLPEKF
                     GRFSDSLWYWAQQTQVQIRRCAVIVRMPIQPKQTSVFRKWMFDGAKVVRRMEGNLWVF
                     GMVALEKTCFKGQMPSENRFQTAFSVYLKQTGKGSNILQSSYSHKRFIVN"
     gene            14765..14856
                     /locus_tag="ACFNMK_001223"
                     /pseudo
     CDS             14765..14856
                     /locus_tag="ACFNMK_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT         3452 a         4128 c         3537 g         3739 t
ORIGIN      
        1 acacggaaaa cgccgccgtc caaaccatgc cgtctgaaga aaactacaca gataccgccg
       61 cttatattac aatcgccgcc ccgtggttcg aaaacctccc acactaaaaa actaaggaaa
      121 ccctatgtcc cgcaataccg aagagctgca aggcatctcg cttttgggta atcagaaaac
      181 ccgatatccg accggctacg cgcctgaaat tctcgaagca ttcgacaaca aacatcccga
      241 caacgactat ttcgtcaaat tcgtctgccc agagttcacc agcctctgcc ccatgaccgg
      301 gcagcccgac ttcgccacca tctacatccg ctacattccg cacatcaaaa tggtggaaag
      361 caaatccctg aaactctacc tcttcagctt ccgcaaccac ggcgattttc atgaagactg
      421 cgtcaacatc atcatgaaag acctcattgc cctgatggat ccgaaataca tcgaagtatt
      481 cggcgagttc acaccgcgcg gcggcatcgc cattcatccg tttgccaatt acggcaaagc
      541 aggcaccgag tttgaagcat tggcgcgtaa acgcctgttt gagcacgacg cacaataaac
      601 catccttccc gtatttcaga cggcattcct tcgatacggt atgccgtctg aacgcttttc
      661 cgaaagattt ttatgtacgc atttaccgac gcacagcaac agaaggcact cttctggctg
      721 gtgctttttc atatcctcat catcgccgcc agcaactatc tggtgcagtt ccccttccaa
      781 attttcggca tccacaccac ttggggcgca ttttccttcc ccttcatctt ccttgccacc
      841 gacttgaccg tccgcatttt cggttcgcac ttggcacggc ggattatctt ttgggtcatg
      901 ttccccgccc ttttgctttc ctacgtcttt tccgttttgt tccacaacgg cagttggacg
      961 ggcttgggcg cactgtccga attcaacacc tttgtcggac gcatcgcgct ggcaagtttt
     1021 gccgcctacg cgctcggaca aatcctcgat atttttgtat tcaacaaatt acgccgtctg
     1081 aaagcgtggt ggattgcccc gaccgcatca accgtcatcg gcaacgcctt ggatacgctg
     1141 gtatttttcg ccgttgcctt ctacgcaagc agcgatgaat ttatggcggc aaactggcag
     1201 ggcatcgcct tggtcgatta cctgttcaaa ctcaccgtct gcggtctgtt tttcctgccc
     1261 gcctacggcg tgattctgaa tctgctgacg aaaaaactga cgaccctgca aaccaaacag
     1321 gcgcaagacc gccccgcgcc ctcgctgcaa aatccgtaac acagccctgc cgggaatcgg
     1381 gacggttggg ttttctttta tttgcaaaca ctgccggaac ggataaatcc ggattcccgc
     1441 cacttcgtca ttcccgcgaa agcgggaatc cggtgcgttg ggtttcagtc atttccgata
     1501 aactcctgcc gcgttgtatt tctagattcc cgctttcgcg ggaatgacgg cggatgggtt
     1561 tccgtgccgt ggtgtccgga ttcccgcccg tacgggaatg acggcagtgc ttccttggct
     1621 taattttatt ttaatcaact gcaaatgccg tctgaaaacc ggtttgcaga ttttcagacg
     1681 gcattgtttt cgtcgccggt tcaaacttca atccgattgc gacacggtaa ccggtatgcc
     1741 gcgcaagcgc gcaagcagcg cgtccgcctt ctgtttctgc gcggcaaacg ccgccttgtc
     1801 cgcctgcgtc aattcgggtg tcggcaatgc gactgaaaca ggattgacgg gctgcccgtt
     1861 gatgcgtgcc tcgtaatgca ggtgcggccc ggtcgaacgc cctgtcgaac cgacaaaacc
     1921 gatgacctcg ccgccgcgca cattgccttg tgcctgcgaa aacgcgctca agtgcgcgta
     1981 cagcgtttcc acgccgttgg cgtggcgtat catcaccgcg ttgccgtatc cgcccttccg
     2041 gcctttaaag gtaatcacgc cgtcggcgga agccctgacc ggcgttccct gcggtgcggc
     2101 ataatcgatg cccgtgtgca gccgccaagt gtgcaggatg gggtgcatgc ggtagccgaa
     2161 cggcgaagaa atgcgcgtat agaccagcgg ctcgatgttg aagccgcctt tttcctgcaa
     2221 caccctgccg tcttcatcgt aataattgcc gccccctccg ccttccttgt ccgaacggta
     2281 atagaacgcc tgatgccttg tgccgccctt aacgacttca gccgccaaaa tatcgcccgc
     2341 cgccacctgc tgcccgtgga aatacaggct gtcgtaaatc aggcgcacgg catcgccttc
     2401 cttcaaaccg tcaaggctga agcggccggc gaaaatcccg cttaaggatt cgcggatttc
     2461 gacgggcact tccgcccgcg ccagcgaacc gcgcgccgac gttttgacca cgaccgaacg
     2521 cagcgtcggc aaaaccttca tatccgcctc agaagccgac cgccgccata tgccgccttt
     2581 cttttccaaa gcgaccagat tgcgctcgcc gtcttcgtcg gtaaaaaact gcacttcgcg
     2641 cgcgccgccg tcgccgccga ccaaaacatg aaccgactgg tcggcacgca aatgccgcaa
     2701 atcggcttcg ccgccatatt tttccgtgat tcgggcaatc tcgtcccgcg ccatacccga
     2761 acgcgccagc acgtccgcca gcgagtcgcc cggctgcacc gcctcctgca cccaatatgc
     2821 cgtctgaacg ccgctgccgc cccaagacag cggcggcaga ttttgttcca cgcgctgcgg
     2881 tctgacgcgc tccgtcctct ctgtcgaagc aatgtatgcc gccgacacca aaataatcga
     2941 aacggcaagc gcacggagcg cgtatttccg atgttttgcc gaaagtggga agacagccat
     3001 attgttcctt tttgacggcg tttgcgccga atcctctata atgttgcgcc tgacctgcat
     3061 ttctgaaacg tcaaacgatg aaactcaccc tcgccctccc ctcgctcaat ttggatgaag
     3121 acgaaatccg aatacctcta tgcctgcacg ccttcaacaa aatcctgcaa tacggctcgc
     3181 cgtatcgcca aagctgcacg gcttcggcat tttacgcgcg ttatttgtgg tgcggcagac
     3241 tggcggaacg cgcggcgcaa agcctgaata tgccgtctga agccgtcgcg ctcgccacgc
     3301 ccgtctggca aaaaatgggg ctgcatcagg caaatgtact gacggcggaa tatctgaacg
     3361 tcggtacgga tgaagccgaa cgcctctgcc gcgatttgtc tgaattttac gccgatactc
     3421 cctggcattt cacgcccgtc ctgcccgaat tatggctggt ttcccttccc cgcgcgtacc
     3481 gctggggcgc gaagcccgtt ttggacttgg gcggattatt gggcgcggac gatcagccgg
     3541 acgggaaaga ctcgttggaa tggctgcgcg tccaaaccga aatccaaatg tggctggcgg
     3601 cacaccccgt caaccataac cgcaagaaac gcgggctgcc cgagttgaac ggcttatggc
     3661 tttgggacag tctgcacgga agcagtcaag gcggcacgct tttcgccgat acggtttgga
     3721 gccgtttcca ccccgaccgc cgcgcattgc ccgacaactt ccgcgcctat gaagaaacgg
     3781 cggcacacct gcccgacacg caccatatcc tgtttatgga cgatttgcgc ctgaccgccc
     3841 tgacagggga cagggagcgg tatgcatcgg ttttacagca gtgggaagaa cactggttcg
     3901 caccgatcta cgaagccgtc cgtaccggca aaatcaaacg gctggacatc accacggacg
     3961 gacaacacgg cggtacgctg accttcaaac ccgccgacag gtggaaattt tggcgcggcg
     4021 cgaaaacctt tgacggaatc tggtgaaccc gcccgccgca accgcgacaa tgccgtctga
     4081 agcccgaatc gggtttcaga cggcatttta cctcgcatcg ccgttatgcc ggaaaaaccg
     4141 gaatgcgttt ccgcacacgc ggcggcacgc aaagcaccgc ccgaaagccg ccgatgcccg
     4201 ccgtgatacc gccgccggca gggtttcctc cgaaaaggaa cagcaatcag aacgttttat
     4261 taaactcgac aaacgcccga tttttctcgt attcgttgaa cacatcgtta ctccgcgttt
     4321 cgcggtgcga caacgtcagg cgcggcgtga tgcctttgaa atgcaatgcc cggtgccaaa
     4381 ggctcaagga tgtgttcaat tctttatccc tgcgcctttc ccctttaaaa ccgctgaaaa
     4441 agccgggttt ttcataatgc cgtttcgccg cgccgaggcg caacagcgaa gacaggccgc
     4501 tgccgcccca ttcctgcccc caggcaaagc gcaggccgta acggttgaaa ttgtcgcctc
     4561 ggtcggcggg gttgcgctcg cggtaaaaat ccaaaccgcc catccaatat tggcgcgcat
     4621 tccggtaaaa caccagcgaa ttggaaattt gcaaatgggt attgtcggaa cgcgcccggc
     4681 gcgtattctt caaacgcccc cactccgccg aagacaacgt ttgccatttc ggggtttgcc
     4741 aacggttgaa ataaaggcgt gcgccgttgg tgtaagaata agcgtcgttg ccgtaggtgc
     4801 ggcgttcgtg gaacactgcc agcccggcat ctttgcgccg gtcggcaaaa ccgatgccgc
     4861 cggaaacgcc tgccgtcata tcgttgaatt tcttattccc cggataaacc ctgccggaca
     4921 cgtcgccgcc cgccgtcgtg taccagccgt ttttcagcga ccattttttc tccgcgccga
     4981 gccggtaatt gaccgccgtg ccgtccacct gtttcgggaa agtccatttg ccgtactgct
     5041 gccgtttcgg ggcttggttg atattgtgtt cgcgggtgac gctgaagccg ccatttacct
     5101 tccacgcatc gcgttcgcgc aatgccttgc ggtacagctc gacctgctcc atcagctgcg
     5161 gcggcaggtt ttccgccttc aggcggtcga actggtctgc cgccgcctcg ttctgcctgt
     5221 tgtcaaacaa tgccgccgcc aaacgcatac ggacggcggg cgcgtcgggt tgggcggcaa
     5281 tcaattcccg gtaatgggaa atcgcttctt tcaccctacc gtctgtctgc gccaaaatcc
     5341 cttgtgcata aagtgccaac atcttatcct gctgcgcctg ttgtaggtaa atcggcaaaa
     5401 taacgcggat accggcaata ttgtttgaga ccactgcgga atacatcgcg cgggacagca
     5461 attcgggatt tttcagcagg gtttcgccgt caatctgcaa aacttttcct ttttcccgca
     5521 cctgccccgg taccttctcc ctgtcgattg gtttgacctc cgcttcatga agcctgaact
     5581 cgggacggct tctcaaatcc ggttcgcgcg gtgtttcttc ggcatatgcc gcgcttgcca
     5641 ataaaggcag cagcagcatc catctgtttc gtgcaggcat aaatgtcttc ccacaaaaat
     5701 aaaaaataac cattatcaac cggcggattg tatcagtttt ttatgccgct gttccaaacc
     5761 ctgccgcccg atgccgcgac aatgccgtct gaagccagaa tcgggtttca aacggcggca
     5821 aaaaaccggc ggttttccgc cggtttcatc atgatgcccc gcccttcggg gcggcagggt
     5881 tcggattggt ttgcggggcg gcgcgcggtg ccgtgccggt gtgccgcctg tacgtcaaaa
     5941 catttcccga cgcatcaaaa cagccatttg agcgacaatg cggcttcttt gtcgccgtcc
     6001 gtccttttgc cgtatccaat gcgtgcggac atatggcctt tccaaccggc ttcaatgccg
     6061 aaccgccctt cgagtgccgt cctgcctgcc agcgtctgtt tttcgccgtc catttccacg
     6121 ccgaaagatt ttgacctgtg caaaacatta aaagcggcaa aaggctgaag attgacaccg
     6181 ttacgcaaag caaaacgggt ttttgcccga atgccagcgc ggctttgcca ctgaccgctg
     6241 ccgagcagtc cgaccgccgt cccctcgctg tcggtaaagc cgccgtttac gcccaagtag
     6301 gtaaactgag cctgcggttg caggtaaaac cgcacattat tgccttttcc gacaacgcct
     6361 tccgccacaa gcgcgttgta gccgccttcg acagaagccg tccaaccttt ggttttgtag
     6421 cgttccgcac ggttttcatc attgatgcga tgtttgaaac gttggtattg caaccagccg
     6481 tccaaatacg cacccgtttg tttatcgcgc aactgatgcc acgcagcata aacacccccg
     6541 ccataaccat gcaaataact gcctgccgca ccgcctttgc cgttgactga tgcgtgctgg
     6601 ccggccctgc cgcccatcac gccgattgcc agtcggctgc cttcattttg ccgtacaaac
     6661 acctcgccgc cgatttgcac gcctttgcgc cgcccgtccg cagccgcgcc gccccgtata
     6721 ttttgatgcg accgaccgcc gatgaagcgc agccacaatt tttgacggga cggatcggcg
     6781 gcatacacgt cgtcgcccct gtcggcggca cgcagcccaa acaaagtatt tgccgcataa
     6841 gcctgttggg catataaaac cgcttccgcc tcggggatag ccaaacgctg cacgcccaag
     6901 caaaatccgc cttcgcattg cttgagcaat aattcgtaca gtccctgccg gtaattttgt
     6961 ttaaacgcaa acgaattaga gtctgccttt tccgccgcaa tcagtttgcg cccgtcgagc
     7021 gttttcagat tttccggccg gcgttccaaa accacgccgg attttccgct gactttttct
     7081 tcaaaggtca gggcgggttc gtccgactcc cgcacgtcgt agccgaacag gaaatccgca
     7141 ccgcccttat acgctttatg gacggtcagt ttttcatctt tcgtgttcag gcggatgcgg
     7201 gcattatcgg aagtttccag cgtatggatg tcggaatggc ggcgcggttc ccaaagggaa
     7261 tctttcagcg tcatcttggt cagttccaac acctgtccgg cgacgtggga tttgttttcg
     7321 attttgacgt gtaatttttt attgaagatg ttgttgagac tagtctgtga tgtattacta
     7381 tgaggagtga gtacggaatc ttctaccaat ttattaatta aatcggggtt tttctgtttg
     7441 agtatactaa attttggtcc aagctgttct atatacgcct cctcagtccg ttttttattt
     7501 tcttcccaag cttcgggtct tgttttgtat aaatcctgta attgcttctt aaccgcatcc
     7561 tttaaattat ccccgtaagt atctgtaata tatttcttaa caacgggcat attatcgacg
     7621 gcgagtatgc cgctattgta gtttttacct tggatattga tttcgtcata cccatattcg
     7681 gatgaatttt gatcggtttt accgctggaa aaataatggg aagtgccggg tttcggcttg
     7741 tctgaaaatg aaaaatcggc atcgtcgccg attttggtag aacgagtatt tgaaccaagc
     7801 tgagctctga acataccgaa gcaatcaaaa gatatgctgc acgggccgcg ccaaacgtgg
     7861 gaatttctgt cttccgccgc ttctaaaata aatttgtttt taatctcggc ttctttgacg
     7921 ggatcgtacg catccgaacc tcaaacatat ggcgtgcaaa acagcgataa aacagataat
     7981 gttaatagat taacccgacc gcttgcttgc ttgcttgctt gcttgcttgc ttgcttgctt
     8041 gctttcagtg tcatgagaaa ttttcctttg tcaagtgtta aaattataat gattatatac
     8101 tacatcacac tacatcgcaa tgaaaaatca ggaaaaacaa aaaacctctc cgccgtcatt
     8161 cccgcgcagg cggaaatcta gaattttaat gcggcaagaa tttatcggaa aaaactgaag
     8221 tttaaagacc tagattcccg cctgcgcggg aatgacggtt tagaaatcac ctgaaacgag
     8281 aaaaagaacc gaaaccggac aagtcggatt cccgcacggg cgggaaggac ggaatttcag
     8341 attgttgttt ttgattttct gttttgtggg aataacggga ttttggatga tgtgcattta
     8401 tcgggaaaac taaaaaccct ccgccgtcat tcccgtgaaa gcgggaatct agaatttcaa
     8461 tgccgcaaga atttatcggg gatgtccggg gtttaaaggt ctggattccc gcctgcacgg
     8521 gaatgacggt ttagaaatca cctgaaacga gaaaaagaac cgaaaccaga taagtcggat
     8581 tcccgcacgg gagggaagga cgggatttca ggttactgtt tttgattttc tgttttgtgg
     8641 gaataacggg attttggatg atgtgcattt tcgggaaaac taaaaaccct ccgccgtcat
     8701 tcccgcgaaa gcgggaatct agaattttaa tgcggcaaaa atttatcgga aaaaaccgaa
     8761 gtttaaagac ctagattccc gcctgcgcgg gaatgacggt ttagaaatca cccgaaacga
     8821 gaaaaagaac cgaaaccgaa caagccggat taccgcctgc gcgggaatga cggctcaaaa
     8881 gttacgaaac gaaaaataac cgaaaccaaa caagtcggat tcccgcgcgg gcgggaatga
     8941 cgataagcgt aatttcaatc tgccatattg tcttttcact tgcctgaaaa cagatttcag
     9001 ccacctatcc ctgcatccgg cggctgatgt agtcggcaat caaatcccgc ttttcccaaa
     9061 aaaacttctg cattttttgt tcggaataca gtttgcggac cagagtgggc aaaccgctgt
     9121 ggacgatggc ggcgatttct ttgttgcctt tttcttcctg cattgccgtc tgaaaatcca
     9181 tacccctgcc tttcagatat tctgcaaggc ggttgcggat gtgggaattg agttcgatgg
     9241 atttgagcat tttgcctccg ttttgcggta aggatttatt cgatttgttt atccgcctga
     9301 ttttatcacg tttcccaagc ggtttgcctg tacaataccg cttttcggca gggtgtgggc
     9361 gatgatgttt tcttggttca agctgtttca cttgtttttt gtcatttcgt ggtttgcagg
     9421 gctgttttac ctgccgagga ttttcgtcaa tatggcgatg attgatgtgc cgcgcggcaa
     9481 tcccgagtat gtgcgtctgt cgggcatggc ggtgcggctg taccgtttta tgtcgccgtt
     9541 gggcttcggc gcggtcgtgt tcggcgcggc gataccgttt gccgccggct ggtggggcag
     9601 cggctgggta cacgtcaaac tgtgtttggg cttgatgctc ttggcttacc agttgtattg
     9661 cggcgtgctg ctgcgccgtt ttcaggatta cagcaatgct ttttcacacc gctggtaccg
     9721 cgtgttcaac gaaatccccg tgctgctgat ggttgccgcg ctgtatctgg tcgtgttcaa
     9781 accgttttga cggagcatct tatgccgata gaaatcgaac gccgtttttt gattgaaaac
     9841 gacaactggc ggcaatacgc cgatgagccg ctgctgttga ggcagggtta tctatctgtt
     9901 gaaaaagaac gcaccgtccg tgtccgtatt gccggacaac gggcgtggct gacgctgaaa
     9961 ggctatattt cggaactcag ccgcagtgag tttgaatacg aaatcccgct tgccgatgcg
    10021 gaaaagatga tggaaacgat gtgtccgttt aagatggaaa aatggcgtta tccggtcaga
    10081 tggggcggca gcctgtttga aatcgatgtt tttcttggcg ataatgcgcc tttggtcgtc
    10141 gcggaaatcg agttgcccga cgagaatgcc gattttgacc gtccggactg gctgggacgg
    10201 gaaatcactg ccgacggtat gtttaccaat gcctatttga gcaggcatcc gttctcaagc
    10261 tggaaaaatg ccgtctgaag cggcttcaga cggcattctc cgacaaccat cgcaaatcca
    10321 aagacagcct ctatatcctt atttctgctt tctttgttcc attattccca ataaatcccc
    10381 ataacctaaa atctcgtcat tcccgcgcag gcgggaatcc ggttctttaa acttcggttt
    10441 tttccgataa attgctttag cttttcgttt ccggattccc gcttttgcgg gaatgacgat
    10501 ggaagattgt tgttatttcg gataaattac cctaacttta aaatcccgga ttcccatctt
    10561 cgggggaata acaatatttt ctatttcagc ccatcatttg cgggggattt tatagagagt
    10621 ttatatttca atatctcaaa taatgcacaa aagcatagcg cgtgcctttg ctgctgacac
    10681 ggcgttccgt ccgctctgct tctttccaat gcgtccggtc tattgcgggg aaaaatgcat
    10741 ctccttccac agacaaatcc acttcggtta tccgcaaatc ggtcgccaat ggcatcgctt
    10801 gtccgtatat ctgcgcgccg cccataatga cggcttcttc cgcgcctgcg cacaatgcca
    10861 atgccgcctc caaacttgcc gccgtttccg cgcctgccgc gcaataatcc gcctgccggc
    10921 tgatgacgat gttcctccgt ccaggcaggg gtttgacggg cagggattcc cacgttttcc
    10981 gccccataat gacgggtttg cccaaggtat aggctttgaa aaatgcgaaa tcttcgggga
    11041 tgtgccaagg catagcattg cccgccccga tgcacaggtt ttccgcacac gccgcaatta
    11101 gggttatttt gagcatattt cctccacaaa aatgccgtct gaacgcttca gacggcattt
    11161 tatgacattc cgtgtcaaaa atctcaaccg gcgcgcgcca tacgtttttt gtttgcacct
    11221 gccaacaatg ccaacagttc ttcagtcgcg ccccaaccga tacacgcatc ggtaatgctt
    11281 ttgccgtaca cttccggctt atcctgtctg ccttcgacca aatggctttc caccatcacg
    11341 cccataatat tgctcccgtc ctgttccaat tgggcagcaa tatcttgtgc cacttccatc
    11401 tgccgggtgt aatccttgcg gctgttggcg tggctgcaat ctatcatcag cttatcggtt
    11461 acccctgccg cacgcagttg ttccgccgcc tcgctgacgt gtcccgcatc ataattcggc
    11521 tcttttccgc cgcgcaaaat gacatgacag tcgggattgc cgccggtatg gacaatggcg
    11581 gaatgtccgg ccttggttac agacaagaaa tgatgcgaat ggctcgccgc accgattgcg
    11641 tcgatggcaa tcttcaaatt gccgtccgta ccgtttttaa agccgacggg gcaggacagc
    11701 ccgcttgcca attcgcggtg aacttggctt tcggtcgtcc gcgcaccgat tgccccccaa
    11761 gaaatcaagt ccgcataata ttgcggcgta atcatatcca aaaactcggt agaggcaggc
    11821 atacccatat tgttcagcga caacaacagg ctgcgcgcct gacgcaaacc gaaattgata
    11881 tcgaacgtac cgtccaaatg cgggtcgtta atcaaacctt tccaacccac cgtcgtcctc
    11941 ggcttctcga aataaacgcg catcacaatc aaaagctcgt tttcatactg cttgcggagt
    12001 ttcaacaaac gctccgcata ttccaacgcc gctttcggat cgtgaatcga acacggcccg
    12061 ataatgacca acagccgctt gtccctgccg tgaaccaaat cggaaatttc ctgacgggtg
    12121 cggtgaacca agcccgaagc ctctttggaa atcggcagct cgtaaagatg ggcaatcggc
    12181 ggcaacagct ctttaacttc tttaatctta atatcgtctg tggggtaatg gtgtgtcatc
    12241 ttgcatcttt cctgatgggt ttttatacgc ggcttaggtt acatcgttta ccttgcacaa
    12301 acaagtgcaa catattacta caaagctatt ttatttcaaa ataactgata tatctttttt
    12361 catagatttt aaatttcata tttttaatat tttaggaatt ttattccaaa gaaaaagaaa
    12421 cagattgata aaaacaatac ccaccattca ttcggacaaa accgcacgga acatccgttt
    12481 tcccatttcc acgacaatcc ggtacagaac accccgctgc cgattccgcc tgcacatctg
    12541 gcaaaatttc atacacaagg aaaatgttga aaacaaaaag aactcattgc ttgaaaaaag
    12601 atttaattgg aatgcgggta tttcccacac tttctccatt tatttatcct cattataaaa
    12661 acatgaaaaa accctgcttg cactctcact tgcagccgtg tccgccgtta cggtagccga
    12721 tattcccgag tctaacggca aaggcaaagc catcatcggc gataaggaaa aacaccgtta
    12781 ccaagcatga aaaccttgct ttgaaagtcc atggcgaaat ccgccaagat ttgggggacg
    12841 gatgatccct aaccccttgg gcggatgcat atgcagattt gcgcggcaaa accaaagtga
    12901 tgacgaccca gatgggtgct tcccgcgatg tcagcaaaag cgccaaaggt tggagtgtcg
    12961 gtatcggtct gaatgtaggc aaacagttga ccgacagcgt cgatctcgag tttgatccat
    13021 actaccgtca caaaacaatc tgcaaacccc gtgagattgt cttggacggt gacaaaacca
    13081 aaatgggccg ctccaaatcc aacgagtacg gcttccgcgt agccgcaacg ttctatagtc
    13141 aattaaaatc aaaataggac agtagtgcat cgtcaaatcg ggcgtaatca gacaaaacgg
    13201 ttcgcagata ccgcttaata ttcgcccaca ctttctcaat cgggttgagc tcaggtgaat
    13261 aaggtgcaag aggcaatacc ttatgtccca atttttccgc catttcccgt aagacaccca
    13321 tacggtgaaa tcacgcatta tctaaaatat agtggattaa caaaaatcag gacaaggcga
    13381 cgaagccgca gatagtacaa atagtacgga accgattcac ttggtgcttc agcaccttag
    13441 agaatcgttc tctttgagct aaggcgaggc aacgccgtac tggtttttgt taggggctgt
    13501 actagattag ccctaaatcc cacaccaatc ccgcaggatt ttaagctgtt gagacggtgt
    13561 gccgaagtta aatcgaaatt cgcattcttt caagaacagc gggaaagatt tacgatcgat
    13621 tccgttgtat tttcgcaaga cgcgttttgc ctgattccaa aagttctcaa tgccgttaat
    13681 gtggttctga cggtctgcaa attccttgga atggttgatg cggtaatgga taaaaccgct
    13741 cacgtccaac ttgtcgcagc tgctcagact atcggtataa acaatgctgt ccggcatgat
    13801 tttctttttg atgacaggga gtaacgtttc agacttggca ttgtttacga caacggtata
    13861 gacccgtccg ttgcgtttca gaatgccgaa gacaaccact tttcctgccg caccgcgacc
    13921 acgtctgcct ttacgccgtc cgccgaaatc gctttcgtcc tgctcgacag ggccctcaaa
    13981 aacctcatcg gcagccaagg ctaaatgatg gttgataacc gtgcggattt tacggtagaa
    14041 cagtgctgcc gaattgggat ggatacccaa aatatcggcg gcagaacggg cggtaacttc
    14101 gagtacaaaa aaacggagca gttctttctg tacttttttc tttaatttgc agtgcgttat
    14161 cttcatattt cgagggtaac atatctgcta atctagtaca gcccctagtt taaagctttg
    14221 tttcttaagt ccgcagagta tgccatggtt agaccttcaa agttgagtat tgtactattt
    14281 tgtttttaat taactataaa acgcttgtgt gaataggaag actgcagaat attgctgcct
    14341 tttcctgttt gctttaaata aacagaaaat gccgtctgaa accgattttc agacggcatt
    14401 tgtcccttaa aacacgtttt ttcaagcgcg accataccaa acacccacag gttgccttcc
    14461 atccttcgta caacctttgc tccatcaaac atccacttcc taaatactga tgtctgctta
    14521 ggttgaatcg gcattcgtac gattacggca caccgcctaa tctgtacctg cgtctgttgc
    14581 gcccaatacc aaaggctatc tgaaaatctc ccaaactttt caggtagcct tatcacgttt
    14641 tcgactgtca ccacacgccc aaagactgcc tgaaaccctt tttcagacgt tccactccga
    14701 taccccccaa caaaacgccg cccgaacaca tgttcagacg acattttctt tgcgtcgatt
    14761 cattgcagtg tttacctgct ttaaggcgga caaaggtgcg gaagcggtaa acaacgatac
    14821 agaacataat gctacatatt ttagccattt cccttc
//
LOCUS       DAXHTY010000049        14371 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXHTY010000049 DAXHTY010000000
VERSION     DAXHTY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14371
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.049"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(75..557)
                     /locus_tag="ACFNMK_001224"
     CDS             complement(75..557)
                     /locus_tag="ACFNMK_001224"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6364497.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(710..871)
                     /locus_tag="ACFNMK_001225"
     CDS             complement(710..871)
                     /locus_tag="ACFNMK_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364498.1"
                     /translation="MSGQPEKHHTSPIEDKRKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(945..1406)
                     /gene="recX"
                     /locus_tag="ACFNMK_001226"
     CDS             complement(945..1406)
                     /gene="recX"
                     /locus_tag="ACFNMK_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC6364499.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1479..2189)
                     /locus_tag="ACFNMK_001227"
     CDS             complement(1479..2189)
                     /locus_tag="ACFNMK_001227"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC6364500.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            2596..3861
                     /locus_tag="ACFNMK_001228"
     CDS             2596..3861
                     /locus_tag="ACFNMK_001228"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFC6364501.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3919..4725)
                     /locus_tag="ACFNMK_001229"
     CDS             complement(3919..4725)
                     /locus_tag="ACFNMK_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC6364502.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            5070..5279
                     /locus_tag="ACFNMK_001230"
     CDS             5070..5279
                     /locus_tag="ACFNMK_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC6364503.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            5368..6582
                     /locus_tag="ACFNMK_001231"
     CDS             5368..6582
                     /locus_tag="ACFNMK_001231"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC6364504.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVYQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(6669..9248)
                     /gene="clpB"
                     /locus_tag="ACFNMK_001232"
     CDS             complement(6669..9248)
                     /gene="clpB"
                     /locus_tag="ACFNMK_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC6364505.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LEKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            9509..10519
                     /gene="trpS"
                     /locus_tag="ACFNMK_001233"
     CDS             9509..10519
                     /gene="trpS"
                     /locus_tag="ACFNMK_001233"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC6364506.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            10525..10866
                     /locus_tag="ACFNMK_001234"
     CDS             10525..10866
                     /locus_tag="ACFNMK_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364507.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            11187..11552
                     /locus_tag="ACFNMK_001235"
     CDS             11187..11552
                     /locus_tag="ACFNMK_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364508.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAKEAVTEAAKDTL
                     NKAADATQEAADKMKDAAK"
     gene            complement(11761..13269)
                     /gene="ppx"
                     /locus_tag="ACFNMK_001236"
     CDS             complement(11761..13269)
                     /gene="ppx"
                     /locus_tag="ACFNMK_001236"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC6364509.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTHSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(13386..14117)
                     /locus_tag="ACFNMK_001237"
     CDS             complement(13386..14117)
                     /locus_tag="ACFNMK_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6364510.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLRLSLGYKNR
                     SVTRSFGIVPFVGQNLLGGSRYNFVGGFNADFSQRLGEHWRLTLNAGNMWKRYQADRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGSADWSHDITKEAEQASIRKGLRVGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTIVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNTQIFMSVEKDFK"
BASE COUNT         3249 a         3565 c         3934 g         3623 t
ORIGIN      
        1 ttgcttgatt tcatccgttt gcaatggaaa cggatggcgc gatgtgcctg tctttcagac
       61 ggcatagtcc gccgtcagca gccgcaggac atgtcttcag aggcttgcag gctgatgtct
      121 ctgcgttttt tggctttttc gtagcggcag cgttggcggt cggtcaggat ttccagcgga
      181 gggacaggga cgggtttgcc gtctttgacc gcaaccatcg tgaagtagca gctgttggta
      241 tggcggattt cgccggtgcg gatgttttgt gcttcgacac ggatgccgat ttccatcgag
      301 gtgcgtcccg tgtagttgac agcggcgtaa aaggtaacca aatcgccgat gtggatgggt
      361 tctttaaaca ggactttgtc aaccgacagg gtaacgcaat aattgccgct gtaacggctg
      421 gcgcaggaat aggcgacttg gtcgagcagg agcaggagtt cgccgccgtg tacgttgccg
      481 ctgaaattgg cggtgtccgg catcatcagt tcggacataa tgagttcgtg cgaaggcagt
      541 tggcgttgtt gtgtcatagt gtacctgttt taagaaaggg tgttttgaaa tgggtgcggg
      601 gaaattttag ggaatgggga agtggaattc aagctaaaaa aagctatggc gggatagtct
      661 gcagaaatgg gccgccgatg ccgtctgaag ccttcagacg gcattttgtt taggaaagcc
      721 aaaaacggaa gaccaggaaa aacacgagtg ccaatacggc gacccaaacg acgagcatga
      781 atccggcaac tgcccgtccc atacggtaaa ccgggttttt gcgtttgtct tcgatggggg
      841 aggtgtggtg tttttcgggt tgtccggaca ttttgttctc aatcggaaaa gttggaaacg
      901 ggcgtaaggt tatgttgccg tgcaaaaaga ttcaagaagg aaggtcagca gccgtcttcc
      961 caattttcgt cccaagcgtg tttcaatgcc gtctgaacgg tatcggcatc gaatccgcga
     1021 taggcgagga aacgcgcctg tttttgtttt tctttgaggt cggcggcggg gtgtttgaat
     1081 tttttacgca acacggctat ggcggcctgt ttttcgcttg agcggtcggg aagcaggttg
     1141 cggctggttt cttcatctat gccctgttgc gccaaagcct gtttcagcct caatgaaccg
     1201 tgtttgcggc ttttgctgcg gatataggct tcggcatagc gcaaatccga ctgccagttg
     1261 cgttcggcaa attcgtttaa cacgttttcc aactcctctt cgctttcggc gtgcggtgca
     1321 agtttgcgtt tcagaccgat gcggctgact tcttggcgcg agaggatgtc catcgcgcgg
     1381 gcgcgtaggg atttttgcgg tttcatggcg gcggtttcgg cacggaaaga ttcagacggc
     1441 atgggaaacg gattccgatg ccgtctgaac agattgtttt attcttcgtc tttatttttc
     1501 tgcggttgca ggttttcgta aatttcgggc agcgagccga taatccagtc ggggcgggtc
     1561 gcatcgtctt gcgagagcag cgtcatatcg ccgtaaccga aggtaacgcc gacgctcagg
     1621 cagccggcgg ctttggcggc gatgatgtcg ttgcgcgagt cgccgaccat aaccatgttt
     1681 gcaacatcga tacccaaaac ttcggcggca tgccgcagcg gcagggggct gggttttttc
     1741 tcgggcaggc tgtcgccgcc gagtatcagg ctgaagtagt cggcaagtcc gagctgtttc
     1801 aacagttcga cggcaaggat ttcgtttttg ttggtaatga ctgccagcgg gatgcccaaa
     1861 gatttgagca atgccagccc ggcttcggtt tcgggatagg ggcgggtgaa gacgctcaaa
     1921 tggtcgcggt agtatttcat ataggacacg aaaccttttt cccacagttc ggaatcggct
     1981 tcgcggtcgc ggtcgttggt gaggacgcgg tgaaccagtt tgccgatgcc gtcgcccaca
     2041 tagctttcga ccactttggc gggcagcggt ttcataccga gttgctccaa catcgcttct
     2101 gcggcggcgg caaggtcggg gacggaatcg cacagtgtgc cgtccaaatc gaaggcgacg
     2161 gcttggacgt gttcgatggc tgcattcata atctgatttc ctgtcgggtt gaaagggttt
     2221 attttaacat cgtttgcaca gggcttctaa tgtatagatt gttaacaatc gcaacaggtt
     2281 ttgcaggcgg tgttttcgga tttcggggcg gaatcgggca agggtttgaa atcccggcga
     2341 tgttccaatt aaatttttaa aaaataaaag gatgcctata tctttattta atttgacatt
     2401 tatttttcgg acgcgcgcgc tgctttttca ggcggagtcc gggctgattc cgtgcggctt
     2461 gaaaaataaa atcatatata gattaatcaa atcgggctta tgccgactac acgctattac
     2521 attttgtcgg gaaattggtt atagtaatga gcgaaatgca cgcccctgaa aatctttttg
     2581 tgaaaaggaa gcaaaatgtc tgaagccctt gcaaaagaaa ccctcaatcc gtttgaaatc
     2641 gcgcgcaaac aggtcaaaac cgcctgcgac cggctgaaaa ccgatccggc cgtttatgaa
     2701 atcctgaaga gtccgacccg cgtgttggaa gtcaatttcc ccgtcaaact cgacgacggc
     2761 acggtcaaaa ccttcacggg ctaccgttcg caacacaaca acgccgtcgg cccctataaa
     2821 ggcggcgtgc gcttccatcc cggtgtgaat ctggacgaag tcaaagccct gtcgatttgg
     2881 atgaccatca aatgctgcgt ggccggcatt ccttacggcg gcggcaaagg cggcgttacc
     2941 ttggatccgc gcgattattc cgaggcggaa ctggaacgca tcgcccgcgc ttattccgaa
     3001 gccatttctc cgctgatcgg cgaaaaaatc gatattcccg cccccgatgt gaacaccaac
     3061 ggcaaaatta tgtcgtggat ggtggatgct tatgaaaacg tggtgaaaca ttccgcaccg
     3121 ggcgtattta ccggcaaacc ggtcgagttc ggcggctctt tggcgcgtac cgaagccacg
     3181 ggttacggcg tgaacctcgc cgccgtccaa gccttggaaa aactgggcaa ggacgtgaag
     3241 ggtgccactt acgccattca aggtttcggt aatgtcggtt accacacagg ttattacgcg
     3301 catcaatccg gcgcgaaagt tgttgcggtt tctactgttg acgttgccat ttacaacgaa
     3361 aacggcttgg atatggaagc tttgtttaaa gagttccaag aaaaaggctt catcaccaac
     3421 gaagccggtt acggcaagga aattaccaac gccgaacttt tggctttgga tgtggacgta
     3481 ctcgcccctt gcgcgctgga aaatcaactg acttccgaaa acgccggtaa agtccgcgcg
     3541 aaaatcgtgg ttgaaggcgc aaacggcccg actacgcccg aagccgacgt tattttgcgt
     3601 caaaacggcg tattggtcgt gcccgatatt ttggcgaact gcggcggcgt ggtggtatct
     3661 tattttgaat gggtacaaaa tctgcaaggc tattattggg agtttgacga agttcaggaa
     3721 aaagaaaccg tcgtcctgcg ccgtgcgttc cgcgatatat ggaacttggc gcaagagtat
     3781 gatgtcgatt tgcgaaccgc gtcttacatg atgagtatcc gccgtgttga aaaagcgatg
     3841 aagctgcgcg gttggtattg atgtttgaac aaaaaatgcc gtctgaagcc ttcagacggc
     3901 atttttgtgc gggcgggatt aatggtgcga atggccgatt tggaaggtta aagtcgagta
     3961 gttcgcctgt ttttggcaca cgctttgatc ggggaagtcg gctttgtgtt cgacattggc
     4021 tttccagaag ccttggcgca aggggatgat gtccacttcg cctttgtcgt ctgtgctgtc
     4081 ggagaaagcc tgtgcttcgg ttttgtgcgt tttgctgcgg tcgctggtgt cgaagccgtc
     4141 aaaggtggcg gtaacggtgg cattgggcag cggttcgcca cggaacagaa cgcggacttt
     4201 gaagcgttcg cctacgtgaa tgttggcggg attgtccagc gggacgattt ccaagttttg
     4261 tccgaccggt ttggtgatga tggcggtgtc cgcgctttcg tgtccgacgt tgacgatgtt
     4321 tttgccgaac attcgggttt gttcgcaata gcttgcgtca ggcatttctt tgatgcccgc
     4381 ctgtttccag cctgctttgt tttttgacca gaaagtaggc tgatattcgg cgatgacgag
     4441 gtaactgccg tccttaacgg gacggttgct tcggtactgg tagttgtatg tgccgcgttg
     4501 aatcatgttt tccttgcctt tttcggtaac cagctgcatc ggtttgctga agatgtgcag
     4561 gcggtctttg gcgatgggtt cgagttcggg aaattcgccg tagcccaagt cggctttaag
     4621 gtattcgccg ccgtgcgtgt gggcggtttc gacccagacg cggtgggcgt gggcggatgt
     4681 ggcaaatagg gcggaaacgg cgagcagtgt caatgttttc ttcatggtgc agcctttgtc
     4741 tcgtgtgggt taaagatttt gttatgttat aacaaaatag gtatcgtgtg aagatggatt
     4801 tgctgtctga aaactgtctt tttgtatagt ggattaacaa aaaccagtac ggcgttgcct
     4861 cgccttagct caaagagaac gattctctaa ggtactgaag caccaagtga atcggttccg
     4921 tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatat
     4981 cgatttgaat ttttcagaaa atgaagcgga cgtttgggcg gaggcaactt gtttgataag
     5041 atagcaatat ttcaaaacgg agaaagatca tgccttatgt caatattaaa gtaaccggcg
     5101 gcaaggaagc accgactgcc gcgcaaaaag cggaactgat cggcggcgta accgaattgc
     5161 tggcacgcgt gctgggcaaa aatcccgaaa caacggttgt cgtgattgat gaagtggata
     5221 ccgataactg gggaataggc ggcaaaagcg tcagcgaacg gcgcaaagag ggcaggtaaa
     5281 aagcctgaaa atctcggttt gatgctttaa attccgcgtg aaaaagagta cattcccacc
     5341 cattgcccaa aatttacgga acacatcatg gataaatttc ccaagtctgc aaagctcgat
     5401 cacgtctgtt acgacatacg cgggccggtt cacaaaaaag cacttcagtt ggaagaggag
     5461 ggcaataaaa tccttaaact caatatcggc aaccctgcgc cgttcggctt tgaagcccct
     5521 gatgaaatct tggtcgatgt catccgcaac ctgccgactt cgcaaggcta ttgcgattct
     5581 aaagggctgt attccgcccg caaagccatt gttcactact atcagaccaa aggtttgcgc
     5641 gatattacgg taaacgatgt ctatatcggc aacggcgtgt ccgagctgat tacgatgtct
     5701 atgcaggcgt tgctcaacga cggcgacgaa atcctgattc ccgcgcccga ctaccctttg
     5761 tggacggcgg cggcaacgct tgcgggcggt acggtacgcc attatctgtg cgacgaagaa
     5821 aacggctggt tccccaacct tgccgatatg gaagccaaaa tcacgcccaa aaccaaagcc
     5881 atcgtcgtca tcaatcccaa taatccgaca ggcgcggtgt acagcaggga aatcctgttg
     5941 gaaatcgccg aactggcgcg caagcacggt ttgattattt tcgccgacga gatttacgac
     6001 aaaatccttt atgacggcgc ggttcaccac catattgccg cgcttgcccc cgaccttttg
     6061 acggtaacgt tcaacggttt gtccaaatcc taccgcgtcg ccggcttccg ccaaggctgg
     6121 atggtgctca acgggccgaa acatcacgca aaaggttaca tcgagggttt ggatatgctc
     6181 tcgtctatgc gcctgtgtgc caatacgccg atgcagcacg cgattcagac ggcattgggc
     6241 ggttatcaga gcattaacga attcattttg cccggcgggc ggcttttgga acagcgcaac
     6301 agggcgtggg aactggtcaa ccagattcct ggcgtgtcct gcgtcaaacc gatgggtgcg
     6361 atgtatatgt tcccgaaaat cgataccgaa atgtaccgca tccgcgatga catgaaattc
     6421 gtttacgatt tgctggtgcg cgaaaaagtc ttgctggtgc agggaacggg gtttaattgg
     6481 atcaagcccg accatttccg cattgttacg ctgccttacg tctatcagat tgaagaggcg
     6541 atgggcaggc tggcaagatt cctgcaaacc taccgccaat aaggggacgg tttgtctgcc
     6601 gaggataaaa aatgccgtct gaaacggaga ttcccgtttc agacggcatt ttcaacagca
     6661 ggaacgaatc aggcaaattt cagtctgtcg ccgtcggctt ccaccctgat ttcgctttcg
     6721 ggcgcatagt ttccggcaag cagggctttt gccagcgggt tttcgatttc cgactggatg
     6781 gcgcgtttga gcggacgtgc gccgtaaatc gggtcgaaac cggctttggc gatgatgtcc
     6841 agagcggcat cggaaacagt caggcggagg ttttgttttt ccaaacgttt ttccaagcct
     6901 ttgagctgga ttttcgcaat gttgcggata ttatcctgat ccagtccgtg gaacacgacc
     6961 acttcgtcga tgcggttgat catttcagga cggaaatgtt ctttcacatc ctccatcaca
     7021 acttctttta ccgcttcgta atcctgaatg cccatttgtt ggatgtgctg gctgccgatg
     7081 ttggaagtca tcacaatcac ggtattttta aaatctaccg tcctgccttg cccgtcggtc
     7141 aaacgaccgt catccaatac ttgcagcagg atgttgaaca cgtcgggatg ggctttttcc
     7201 acttcgtcca aaagaatcac gctgtacggt ttgcggcgga cttgctcagt caggtagccg
     7261 ccttcttcgt agccgacata gcccggaggc gcgccgatta agcgggcaac ggcgtgtttt
     7321 tccatatatt cggacatatc gatacgaatc agatgatctt cgctgtcgaa cagaaagcct
     7381 gccagggctt tacacaactc ggttttaccc acgccggtcg ggcccaagaa caggaagctg
     7441 ccgtaaggtt tgttcggatc ggcaagaccg gagcggctgc ggcggatagc gtcggacacg
     7501 gcacgcacgg cttcgtcctg tccgaccacg cggcggtgca atacttcttc cattttcagc
     7561 agtttgtcgc gttcgccttc catcattttg gatacgggaa tgccggtcat acgggaaacc
     7621 acctctgcga tttcctccgc gccgacatta ttacgcaaga gtttgtttgc cggttttgtg
     7681 ctgtccgtat ctgcccgttc ggcggctgca cgctgttttt ccaaatgctc caaatcttca
     7741 tacatcaatt ttgaagccag tgccaaatcg ccttgccgtt ttgcctgttc gattttaatt
     7801 ttgacttcgt caatttgttt cttaatatta gcagcaccgt ctgaaattgc tttttcggct
     7861 ttccagattt cgtccaaatc ggcatattct ttttccaaac cgtcgatttc ctcatctatc
     7921 agttccaaac gttttttgct ggcatcgtct ttttcttttt caacgtgcgc cttttccatc
     7981 cgaagctgaa ttagacggcg gtcgattttg tccattgctt ccggcttggt ttctttttcc
     8041 atcttgacac ggctggcggc ttcgtcaatc aaatcaatcg ctttatcagg caggaagcgg
     8101 tcggtaatgt agcggtcgct caactccgct gcggcaacga tagcagggtc ggtaatatcg
     8161 ataccgtggt ggatttcata acgctcctgt aaaccgcgca agatagcgat ggtgtcttct
     8221 acacttggct cgccaaccaa tactttttgg aagcggcgtt cgagtgccgc gtctttttcg
     8281 atgtattggc ggtattcgtc caaggtggtc gcgccgatac agtgcaattc gccacgtgcc
     8341 aaagccggtt tcagcatatt gcccgcatcc atcgcaccgt cggttttgcc tgcgccgacc
     8401 aaagtatgga tttcgtcgat gaaaatcaga gtgttgccgt cgtctttcgc caagtcgttc
     8461 aacacgcctt tcaagcgttc ttcaaattcg ccgcggtatt tcgcgccggc aatcaatgcc
     8521 gccaagtcca aaaccagtag gcgtttgttg cgcagggatt caggtacttc gccattgacg
     8581 atgcgttgtg ccaaaccttc gacgatggcg gttttaccca cgcccggttc gccaatcagc
     8641 acagggttgt ttttggtacg gcgttgcaat acctgaatcg cacggcggat ttcgtcgtca
     8701 cggccgataa cggggtcgag cttaccgtct cgggcgcgtt gggtcagatc gagcgtatat
     8761 tttttcaaag catcgcgttg gtcttcggca ttggcatcgt tcacgttttg tcctcctcgt
     8821 accgcgtcaa tcgcggcatt gatgttttgt tcggtcgcgc cggcttcttt caaaattttg
     8881 ccggccgcat cgttctgctg caccaaggca agcaggaaaa gttcactggc gatataggca
     8941 tcgccgcgtt tggttgcggc tttgtccatc aggttcaaca ccgcctgcaa ttcgcggctg
     9001 ggcagaatgt cgccgccctg gccggacact ttcggcaggc tgttcaaatg ctgctgcaaa
     9061 cgctgtttca cctgcggcac gttcacgccc gcatgagcca agagcgcggc agcaccgctg
     9121 ttttggtcgt caagcagggc tttcagcaca aagcccgctt ccagatagct gccgtccgca
     9181 gccaacgcca aactctgagc ttctgcaagg gcctgttgga atttggcggt taatttgtcg
     9241 tatcgcattt ttgtttcctt ttcaaaatgt ccgctgtcga agcctatatg tgcataattg
     9301 tggataactc aagttctgtt ttctgttttt ctatatttaa ttcgatatat cattgaattt
     9361 aaagtatata aaaatgtata ataatgtgta taactatatc ttcttaatat ggaaaagtct
     9421 gttgtcggct ggatgtaggt ggcaaatcgg gtataatcgg cgcatctttt tccctttcag
     9481 acggcattga tgccgcaagg acatttttat gagcaaaaaa cgagttctga ccggcgtaac
     9541 caccaccggc atcccgcatc tgggcaacta cgtcggtgct atccgccccg ccgtccgcgc
     9601 ggcgcaaaac cctgataccg aatccttcct cttcctcgcc gattaccacg gtatcatcaa
     9661 atgccacgaa ccggagatga ttcaccaatc cacccaagcc gttgccgcca cttggcttgc
     9721 ctgcggactc gaccccgagc gcaccacctt ctaccgccaa agcgacattc ccgaagtgat
     9781 ggaattgaac tggattctga cctgcatcac cgccaagggt ttgatgaacc gcgcccacgc
     9841 ctacaaagcc gccgtgcagg caaatgcgga aaacgggcag gaagaccccg atttcggcgt
     9901 ggaaatgggt ttgttcagtt atccgattct gatgactgcc gatattctga tgttcaacgc
     9961 caacgaagtc cccgtcgggc gcgaccaaat ccaacacgtc gaaatggcgc gcgacatcgc
    10021 cggccgcttc aatcaccgct tccgagaact cttcaccctg cccgaagtga aaatcgacga
    10081 aaacgtcgaa ctcttggtcg gtttggacgg acgcaaaatg tccaaatcct acggcaacac
    10141 cattccgctt tgggaaaacg acaaaaaaac ccaaaaatcg gtcaacaaaa tcatcaccaa
    10201 tatgaaagag ccgggcgagc cgaaacagcc cgacgaaagc ccattgtttg aaatctacaa
    10261 agccttctcc acgccgtctg aaacggtgga atttacgaaa atgcttgccg acggcctggc
    10321 ttggggcgag gcaaaaaaac ttttggcggc gaaaatcaac gccgaactcg ccgaaccgcg
    10381 cgaacgctac aacgagctga ccgccgaccc ttcgcaaatc gaagagattt tacaggcagg
    10441 tgcgcaaaaa gcgcgtaaag aagcacgcga attgttggac aaagtacgcg atgcggtcgg
    10501 catccgcccg ttgaaataaa cccgatgccg tccgaacctc ttaaaggctt gccggaacaa
    10561 aacgtcgtcc ggctgaccgg caagcatccc aacgacttgg aagccgtcag cggcaaatgc
    10621 atggaaaccg acgataaggg cggtccggta ggctgggcgg aaaacggcgt gtgccatacc
    10681 ttgtttgcca aactggtggg caatatcgcc gaagacggcg gcaaactgac ggattacctg
    10741 atttcgcatt ccgccctgca accctatcag gcaggcaaaa gcggctatgc cgccgtgcag
    10801 aacggacgct atgtgctgga aatcgacagc gagggggcgt tttatttccg ccgccgccat
    10861 tattgaggta ttcggacatc ccggaatata tttgggtttt tcaaaccttg cgaaaaaaag
    10921 tccgcaccgc agggaaactc aaaaggaggg ggatatgtgt tacaattttc cgaactgttt
    10981 tcataaaata gttttcggac gtgtttcaat atggcattga tgccgcctta ttgttcgaga
    11041 aaaaaaacct ttatatttaa atataatggg ttttaactaa acgggaaacc gttttctctc
    11101 cggccgatgg gcaaaatcag ccgattgatg gaacacggtc cgatttttaa gcaaaacctt
    11161 aatcaacaac tcaagaagga taagacatga aaaaattatt gattgccgca atgatggcgg
    11221 ctgccttggc agcttgttcg caagaagcca aacaggaggt taaggaagcg gttcaagccg
    11281 ttgagtccga tgttaaagac actgcggctt ctgccgccga gtctgccgct tctgccgtcg
    11341 aagaagcgaa agaccaagtc aaagatgctg cggctgatgc aaaggcaagt gccgaggaag
    11401 ctgtaactga agccaaagaa gctgtaactg aagccaaaga agctgtaact gaagccaaag
    11461 aagctgtaac tgaagcagct aaagatactt tgaacaaagc tgccgacgcg actcaggaag
    11521 cggcagacaa aatgaaagat gccgccaaat aatttgttgc cttggcaaaa atgatgggat
    11581 gccgcctgcc ggcaacccaa acgaaaccgc ctgaagattt tcaggcggtt tttgagttgt
    11641 atgccccttg ttttttacgc ctgcatgacc gttccgagca gatgtgccgc gcatatcggg
    11701 atgttgcgga cagggaaatg ccgtctgaac atcattcaga cggcatttgc cccgcaaggt
    11761 tcagacggcc tcgactttga acggcatatt gattttttgc cattggacgc tttcatattc
    11821 caatgcgtcg gcaatcaggg gatggcgttc cagccattcc ctgtcaatac gcaggatgaa
    11881 gccgcagctt tccgtgtccg tacgcaactg catatttttc gggaaagaca ggtcttggcg
    11941 cgaacggcag aacagtgcgg caaggcgcag cgacaaaacg gcataccaca acatttcgtt
    12001 ggtgccgatg atgccgctca tttttttcat atcgccgcga tgaccgatga ccagttgggc
    12061 aagtatggtc tgttctttgc gtgagaaacc cggcatatcg gcgttttcga ggatgtaggc
    12121 ggaatgcttg tgatagccgg tgtgggcgat gtccaaaccg atttcgtgca gcgcggcggc
    12181 gcgtccgaga tactgttgcc acaaggcaag ctcttgaact gtaacgtttt tagcgtggca
    12241 gaggctgtcc ataaaggttt gcgcggtctc ggcggtgcgt ttcgcctgat tgaggctgac
    12301 gtggtagcgg tgttggaact cggcaaccgt ttgtccgcgc atatcttcgt ttaaaccgcg
    12361 cccgatcaaa tcgtaaaaca cgccgtcgcg cagggcggct tcggttacgg tcatcctgtc
    12421 gagtttcatt tcctcaaacg ccgccatcat cacggcaagt ccgccggcaa aaacttcgat
    12481 gcgttccggt ttcaggtttt caaatttggc ttttttgacc gaaccggctt cgatgatgcg
    12541 ttcggcgagg gcgcgcattc ctttgtaggt aatgtccgcc tcttggggca tttcggcggc
    12601 aagcacgtcg cggatggatt ttgccgaacc cgatgtgccg acggcgaaat cccaaccttc
    12661 gcgcctcata tttttgctga tgcgctggat ttcgttgcgg gcggcggaaa tggcagattg
    12721 gaagtctttg gcggtgattt tgttttggaa gaagcgcagg ctgtgggtta cgcagcccaa
    12781 gggcaggctt tcagtaatgt cgggattcag cgtcgagccg atgacaaatt ctgtcgaacc
    12841 gccgccgatg tcgataacca gcattttgcc gccgcccggg gggagggtgt ggatcacgcc
    12901 ggtataaatc agccgcgcct cttcgcgccc ggcgatgatt tcgatgggga aacccagtgc
    12961 cgcttcggct ttgggaagga aatctgcgat gtttttggcg acgcggaatg tattggttgc
    13021 cacggcgcgt acctgttcag ggcggaagcc gcgcaggcgt tcgccgaatt ttgccagaca
    13081 gtccaaagcc tgttcttggg aagcggcact caggtttttc tgttcgtcca gtccggcggc
    13141 gaagcgcacc atctgtttga acgaatcgat gactttcaac tgtccgttgt tgttttcgca
    13201 aatctggagg cggaaactgt tggaacccaa atcgacggag gcgaggatgt ttgcgggggt
    13261 ggtggtcatg gcggatatcg gtgggggaaa aacgcaatgt tactctgacg gcgcaggcgt
    13321 tgacaataaa tgatgaggtt gtttttgatt ctgcccacgg atgttgccga cggcattttt
    13381 tgcgcttatt tgaaatcctt ttccacgctc atgaaaatct gcgtgttttt gcgtgtgtaa
    13441 aaacttttca tattgctgtc gattttcaga tagcggaaat tgagttgcgg cgtaaatccc
    13501 ttccaagaga ttttgtcgtg ccacaacgac aggtttgcct gatattcgtg gtctttacgc
    13561 gggaagcggt acacaatggt cccgggtgca tcaaaagtcc tgcgggtata gcgcaggttt
    13621 gcccgcagac ccagcccgcc gtcgaacgtt ttgaccgcgc cgacacgcaa acccttgcgg
    13681 atggaagcct gttccgcctc tttcgttatg tcgtgcgacc agtccgcact gccgtaaagc
    13741 agccagtctt tcggcgcgga atacatcagc gtcgcacccg ccagcggcat atggctgtcg
    13801 tatcgggcgg cggtgcggtc tgcctgataa cgcttccaca tattgcccgc gtttagtgtc
    13861 agccgccagt gttcgcccaa gcgttgggag aaatcggcat tgaagccgcc gacgaaattg
    13921 tatcggctgc cgcctaagag gttttgcccg acaaacggca cgatgccgaa cgagcgcgtt
    13981 accgaacgat ttttatagcc gagcgacagg cgcaggcact gttcgctgaa atctttgtta
    14041 tcccaataat gcacgccgcc gccgctgatg ccgccgtaga ggaaatgatg cccgcccgca
    14101 ttgatttcgc gcgacacgcc taagccctag cgcaaaccgt gtgccttttg cggcaggctg
    14161 tcggcggttt tcgtccagct tctgcccgca aattcaatcg tttttttgga cgaagcgttg
    14221 tttatagcgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacaaatagt
    14281 acggaatcga ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc
    14341 gaggcaacgc cgtactggtt tttgttaatc c
//
LOCUS       DAXHTY010000050        14191 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXHTY010000050 DAXHTY010000000
VERSION     DAXHTY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.050"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(122..1447)
                     /locus_tag="ACFNMK_001238"
     CDS             complement(122..1447)
                     /locus_tag="ACFNMK_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC6364511.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPAPAGTTVAGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFF
                     RELTGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1658..2497
                     /gene="efeU"
                     /locus_tag="ACFNMK_001239"
     CDS             1658..2497
                     /gene="efeU"
                     /locus_tag="ACFNMK_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFC6364512.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            2494..3660
                     /gene="efeO"
                     /locus_tag="ACFNMK_001240"
     CDS             2494..3660
                     /gene="efeO"
                     /locus_tag="ACFNMK_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC6364513.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIETKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3924..5189
                     /gene="efeB"
                     /locus_tag="ACFNMK_001241"
     CDS             3924..5189
                     /gene="efeB"
                     /locus_tag="ACFNMK_001241"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC6364514.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKRGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRRF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5261..5593)
                     /locus_tag="ACFNMK_001242"
     CDS             complement(5261..5593)
                     /locus_tag="ACFNMK_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC6364515.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNKTESAAAEAAEEV
                     RDANGAALQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIGCKIDGSAMNL
                     KSEFVKKA"
     gene            complement(5670..7040)
                     /gene="glmU"
                     /locus_tag="ACFNMK_001243"
     CDS             complement(5670..7040)
                     /gene="glmU"
                     /locus_tag="ACFNMK_001243"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC6364516.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLATEGRTLVLY
                     GDVPLIDVETLKTLLEAAGGEVGLLTDVPADPTGLGRIIRDGNGSVTAIVEEKDANAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPIQVRA
                     SYLAAGVNNKLQLAELERIFQTEQAQELLKAGVTLHDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYTRLRPQ
                     AKLANDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVSPVKIGNKVTTGAGSTITRNVEDGKLALARSRQTIIDGWVR
                     PEKNKQ"
     gene            complement(7095..7367)
                     /locus_tag="ACFNMK_001244"
     CDS             complement(7095..7367)
                     /locus_tag="ACFNMK_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC6364517.1"
                     /translation="MRKTFLILMTATALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTDGSEQGVRGQNWQKPKFQNR"
     gene            complement(7393..8073)
                     /locus_tag="ACFNMK_001245"
     CDS             complement(7393..8073)
                     /locus_tag="ACFNMK_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC6364518.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGLFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIADFLRESHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVWFGAKSHALPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTP"
     gene            8132..9133
                     /locus_tag="ACFNMK_001246"
     CDS             8132..9133
                     /locus_tag="ACFNMK_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6364519.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIAHFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9227..10075
                     /locus_tag="ACFNMK_001247"
     CDS             9227..10075
                     /locus_tag="ACFNMK_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC6364520.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10101..10586
                     /locus_tag="ACFNMK_001248"
     CDS             10101..10586
                     /locus_tag="ACFNMK_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC6364521.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10688..12256)
                     /gene="msrAB"
                     /locus_tag="ACFNMK_001249"
     CDS             complement(10688..12256)
                     /gene="msrAB"
                     /locus_tag="ACFNMK_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC6364522.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12401..13666
                     /gene="ftsY"
                     /locus_tag="ACFNMK_001250"
     CDS             12401..13666
                     /gene="ftsY"
                     /locus_tag="ACFNMK_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC6364523.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKEAVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            complement(14012..>14191)
                     /locus_tag="ACFNMK_001251"
     CDS             complement(14012..>14191)
                     /locus_tag="ACFNMK_001251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364524.1"
                     /translation="YGGIKRGMAENLPFPSFIHLLFLEFAFTGRGGAPRTPGFGKPPD
                     SAPAELLVEKLTSPF"
BASE COUNT         3044 a         3626 c         4267 g         3254 t
ORIGIN      
        1 aatttgatcc actataattc cgtcaaataa gaaaggaatt ttgtgcctgc ggtatcgcaa
       61 aacttcgcct taatgcgccc gattgcctag ggatgggctt cagacggcat tgttttccgg
      121 tttacgggcg gtattcgggc ttcataccgt tgggcagaag ctgccagaca tatcccgtgg
      181 ttttctgttt gccggcaagt tcgccggctt cgtcgccgta accccaaaaa taatccacgc
      241 gcaccgcgcc tttaatcgcg ctgccggtat cctgcgccat aatcaggcgg ttgagggctt
      301 tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc gcccaaggta atgtagtgcc
      361 ggtcgactgc gccggcgtac tcgcccatca gcggcgtgcc cagtgcgccg acagggccgt
      421 cattgctgct tccggtaagc tctcggaaaa agatatagct ggggttttgc cccaaaactt
      481 cggcgaggcg ttgcgggttt tgctgcatat aggctttgat gccctgcatc gaggtctgcc
      541 cgagcttgag gtagcctttg tccgccatat agcgtccgat ggaaacgtag ggatgttcgt
      601 ttttgtcggc atagccgatg cggatgtatt tgccggacgg ggttttcaga cggcccgagc
      661 cttggatgtg cataaaaaaa agttcgacgg ggtcttcggc gtaaccgagt atcggggctt
      721 tgccgtcaag cgcgccgccg ttgatttggt tgcgcgtgtg gtaggggagg aagcggcttc
      781 cttcaaacct gcctttgatt gccgttgtgc gcgcggtgat ggggaatcgg gagaggtcgg
      841 cggtgtgcgt gccgccggca ttgtcgattg tgccgctgtt tttccccgtc tgcctgatgc
      901 ggacaagggc ttttccgctc cgcaaaccgg caggcagggg gacggagata aaatcgtcgg
      961 gaataccgta aatcgggaag cgggcttgtg ccgtccgcct gtcgtcgccc ttcagcaccg
     1021 gctcgtaata gccggtaacc gtgccggcaa ggcttccgtt gcctgcaacc tgccacggcg
     1081 tgaaatagcg ttcaaaaaac tgttttgcct gaaaggaatg gacgggggtt tgaaaggctt
     1141 gggcacacac atcctgccag ccttggcggt ttttcaaatt ggcgcagccg aggcggaagg
     1201 attgcaggct tttggcgaaa tcctgtgccg cccagtgggg cagggacagg tgcggcacaa
     1261 cggtataaac ggccccgccg ccggcaaccg tcgttccggc gggggcgggg atgccgaccg
     1321 gccggtccgg gccgttgatg acggatgtgt cgggttgcgg aaaggtttgg atgctcttgc
     1381 tttggcaggc ggcgaggatg gcggcggcga tgccgcacag ggcggcgcgg aacaggtatt
     1441 ttttcataat ggacaatgtt gccggcagta ataagaaaga tggtttcggg cggcgttgcg
     1501 gcagccgtgg agaggggatt ttaacacagg gcgcagctgc agcctgcgga actttccgcc
     1561 gcgcggtact gcagataaaa ataacttgca tttgtattta caagcaatga aaatattccg
     1621 ataatatatt attcatcatc cttgttcgtt cgcgtttatg ctggttgctt ttttaattat
     1681 gttgcgcgag ggtatcgaag ctgcactgat tgtcggtatc gttgcgggtt ttttgaaaca
     1741 gtccggacat tccaaactga tgcctaaggt ctggttcggg gttgtccttg cttctttgat
     1801 gtgtttgggt atcggatacg gcatccattc ggcaacgggc gagattcccc agaagcagca
     1861 ggagttcgtc gtcggtatta tcggtttggt tgccgtcgcg atgctgactt atatgatttt
     1921 atggatgaaa aaggcggcgc gttcgatgaa gcagcagctt caggactccg tgcaggcggc
     1981 cttgaaccgt ggcagcggtc aaggatgggc cttggtcggt atggcgtttc ttgctgtggc
     2041 gcgcgaaggt ctggagagcg tttttttcct gcttgccgta ttcaaacaga gcccgacgtg
     2101 gcagatgccg gccggtgcgg tagcgggggt tttggctgcc gccgtgattg gcgcgttgat
     2161 ttatcagggt gggatgcgcc tgaatctggc gaagtttttc cgttggacgg gggcgttttt
     2221 gattgtcgtt gccgccggcc tgcttgccgg ctcgctgcgc gcgctgcatg aggcaggtat
     2281 ttggaacgcg cttcaggaca ttgtgttcga ctcatcaaaa tatttgcacg aagacagccc
     2341 gttgggcgtg ctgctcggcg gatttttcgg ctataccgac catccgacgc agggcgaggc
     2401 cttggtttgg ctgctgtacc ttattcccgt cataacttgg tttttgtgcg gcagcaggcc
     2461 gtctgaaact ttaacccgta aagaggagct gaaatgagaa aattcaattt gaccgcattg
     2521 tccgtgatgc ttgccttagg tttgaccgcg tgccagccgc cggaggcgga gaaagctgcg
     2581 ccggcagcgt ccggtgaggc gcaaaccgcc aacgagggcg gttcggtcag tatcgccgtc
     2641 aacgacaatg cctgcgaacc gatggaactg accgtgccga gcggacaggt tgtgttcaat
     2701 attaaaaaca acagcggccg caagctcgaa tgggaaatcc tgaaaggcgt gatggtggtg
     2761 gacgagcgcg aaaacatcgc ccccggactt tccgataaaa tgaccgtcac cctgttgccg
     2821 ggcgaatacg aaatgacttg cggtcttttg accaatccgc gcggcaagct ggtggtaacc
     2881 gacagcggct ttaaagacac cgccaacgaa gcggatttgg aaaaactgtc ccaaccgctc
     2941 gccgactata aagcctacgt tcaaggcgag gttaaagagc tggtggcgaa aaccaaaacc
     3001 tttaccgaag ccgtcaaagc aggcgacatt gaaaaggcga aatccctgtt tgccgacacc
     3061 cgcgtccatt acgaacgcat cgaaccgatt gccgagcttt tcagcgaact cgaccccgtc
     3121 atcgatgcgc gtgaagacga cttcaaagac ggcgcgaaag atgccggatt taccggcttc
     3181 caccgtatcg aatacgccct ttgggtggaa aaagacgtgt ccggcgtgaa ggaaattgca
     3241 gcgaaactga tgaccgatgt cgaagccctg caaaaagaaa tcgacgcatt ggcgtttcct
     3301 ccgggcaagg tggtcggcgg cgcgtccgaa ctgattgaag aagtggcggg cagtaaaatc
     3361 agcggcgaag aagaccggta cagccacacc gatttgagcg acttccaagc caatgtggac
     3421 ggatcgaaaa aaatcgtcga tttgttccgt ccgctgatcg agaccaaaaa caaagccttg
     3481 ttggaaaaaa ccgataccaa cttcaaacag gtcaacgaaa ttctggcgaa ataccggact
     3541 aaagacggtt ttgaaaccta cgacaagctg ggcgaagccg accgcaaagc gttacaggcc
     3601 tctattaacg cgcttgccga agaccttgcc caacttcgcg gcatactcgg cttgaaataa
     3661 gccgcaagcg ttcagacggt atttggcggc agataccgtc tgaagtttta tagtggatta
     3721 acaaaaacca gtacggcatt gcctcgcctt gccttgccgt actatttata ctgtctgcgg
     3781 cttcgtcgcc ttgtcctgat ttttgttaat ccactatatc cgccatatat tgcagggcgg
     3841 gatttcaacc tgccgctatc ggttaatgga aaaacggcgt gcagggatac ccatcctgct
     3901 gcacggatat tgaaggaaac accatgagca aaaaccaacc cgcacaaccg accaggcgca
     3961 ctctttttaa aaccgcgatc gcagccggag cagtcggcgc aatcggaggt tatctcggcg
     4021 gcaaaaaacg gggcgaaacc gccgaacgca ccgccgaaag ccaacactcg ccccaagcct
     4081 atccctgcta cggcgaacat caggcaggca tcgttacgcc gcagcaggcg ttttcgatta
     4141 tgtgcgcctt cgacgtaacc gcgcaaagtg ccaagcagct ggaaaacctg ttccgcacgc
     4201 tgaccgcccg catcgagttt ctcacccaag gcggcgaata ccaagacggc gacgacaaac
     4261 ttccgccagc cggcagcggc attttgggca aagccttcaa ccccgacggg ttgaccgtta
     4321 ccgtgggggt gggcagcagc ctgtttgacg gccggttcgg actcaaagac aaaaaaccga
     4381 ttcatttgca ggaaatgcgc gacttctcca acgataagct gcaaaaaagc tggtgcgacg
     4441 gcgatttgag cctgcaaatc tgcgccttta cccccgaaac ctgccaagcc gccctgcgcg
     4501 acatcatcaa acacaccgtc caaaccgccg ttatccgttg gagtatcgac gggtggcagc
     4561 ccaaatccga acccggcgcg atggcggcgc gcaacctgtt gggcttcagg gacggcacgg
     4621 gcaaccccaa agtttccgac cccaaaactg ccgacgaggt tttgtggacg ggggtggccg
     4681 ccaacagcct cgacgaaccg gagtgggcga aaaacggcag ctatcaggca gtccgcctta
     4741 tccgccgctt tgtcgagttt tgggacagga cgccgcttca agagcaaacc gacattttcg
     4801 ggcggcgcaa atacagcggc gcgccgatgg acggcaaaaa agaagccgac caaccggatt
     4861 ttgccaaaga ccccgagggg aataccacgc ccaaagacag ccatatacgc ctggcgaatc
     4921 cgcgcgatcc cgaattcctc aaaaaacacc gcctcttccg ccgcgcctac agctattcgc
     4981 gcggactcgc ctcaagcgga cagcttgatg tcgggctggt gttcgtctgc tatcaggcaa
     5041 accttgccga cggattcatc ttcgtgcaaa acctcctcaa cggcgaaccg ctggaagaat
     5101 acatcagccc cttcggcggc ggctatttct tcgtcttgcc cggcgtggaa aaaggcggct
     5161 ttttggggca agggctgctg ggcgtataaa tccgccatat aaaaaacgcc gtccgaacct
     5221 tgccaaacgg gttcggacgg cgtttcttgt ttttgggcgg tcaggctttt ttgacgaatt
     5281 cggattttaa attcatcgcg ctgccgtcga ttttgcagcc gatgttgtga tcgccttcct
     5341 gcaggcgtat gcctttgact tttgtacctt gtttgatcgt catcgagctg ccttttacct
     5401 tgaggtcttt gatgaggatg acggtatcgc cgttttgcag tgccgcgccg ttggcatcgc
     5461 gcacttcttc ggcggcttcg gctgcggcgg attcggtttt attccattcg tgggcgcatt
     5521 cggggcagat gtattgtccg ccgtcttcgt aggtgtattc ggaggcgcat tgtgggcatg
     5581 ggggtaatga catggtttgc agtccttatc tgatatcgga ggagtttgat gccgtctgaa
     5641 acctgaaacc ggcttcagac ggcatagctt tattgtttgt ttttttcagg cctcacccag
     5701 ccgtcgatga tggtttggcg ggagcgggcg agggcgagtt tgccgtcttc gacattgcgg
     5761 gtaatcgtgc tgcccgcgcc ggtggttact ttgttgccga ttttgacggg ggagaccaag
     5821 acgcagtttg aaccgatgcg cacttcgtcg ccgatgacgg ttttgtgttt gtgcacgccg
     5881 tcgtagttgg caataatcgt acctgcgccg aagttggttt tgctgccgac ttcggcatcg
     5941 ccgatgtagg tgaggtggtt ggctttggtg cctttgccga tggcggcgtt tttgatttcg
     6001 acgaagttgc cgacgtgtac gtcgttagct agtttggctt gcggacgcag gcgggtatag
     6061 gggccgattc ggttgttttc gccgacttcg cagctttcga ggtgggagaa gggggcgatt
     6121 ttgctgtttg cgccgatttt ggcgtttttg atgacgcagt ttgcgccgat ttcgacgttg
     6181 tcgccgagtt cgacttcgcc ttcaaagata cagttcacat caatcacgac atcttgtccg
     6241 tgtttcagac ggcctcgtaa atcgaaacgt gccgggtcgt gcagggtaac gcctgctttg
     6301 agcaattctt gcgcctgttc ggtttgaaag atgcgttcga gttcggcgag ctggagtttg
     6361 ttgttcacgc cggcggcgag gtaggaggcg cggacttgga tgggatgaac tttaataccg
     6421 tcggcaacgg ctttggcgat gaggtcggtt aaataatatt cgccttgggc attgttgctg
     6481 gaaaggctgt tcagccagtt ttccagtttg gcgttgggta gaaccaaaat gccggtattg
     6541 atttctttca cggctttttg ggcggcgttt gcgtcttttt cttcgacaat ggcggttacg
     6601 ctgccgttgc cgtcgcggat gatgcgcccc aagcctgtcg ggtcggcggg aacgtcggtc
     6661 aacagtccga cttcgccgcc ggcggcttcg agcagggttt tgagggtttc aacgtcaatc
     6721 aggggaacgt cgccgtacag caccagcgtg cggccttcgg tggcaaggtg gggcagggcg
     6781 gttttgacgg cgtggccggt gccgagctgt tcggtttgtt caacccagac gacatcgcgt
     6841 ttgacggtgt ctaaaacttg ctctttgccg tggccgacga cgacgcagat gttttgcgga
     6901 ttcagcgcgg cggcggtgtc gataacgcgc ccgagcatgg gcttgccgcc gatgcggtgc
     6961 agcactttgg gcattttgga atacatgcgc gtgcctttgc cggcggcgag gataacgatg
     7021 tttaaagtgt tttgcggcat gacggtttcc tgtgcgatgc cgtctgaaag ggcttcagac
     7081 ggcatagggt gggtttatcg gttttgaaat ttgggctttt gccagttctg tccgcgtacg
     7141 ccttgttcgg aaccgtcggt ttggttgggc agcacggcat ggcgttcggg gcggtattgg
     7201 ttgtagttca tatttttcga gtagctgccg tcttggtaat aaacgggcgt gccggcagga
     7261 tatttttgac ggacggcggt tttgccgttg ccgtcttgat aagtttccca cgcgcagccc
     7321 gacaaaaggg cggtggcagt catcaggata aggaaggttt tacgcatggc ttttctttcg
     7381 tattttcggg ggttaggggg tattgtaatg attttggcgg tgttctgaca aagtttctgc
     7441 ataccgagcc agttgcgcca tatcgcttac ggaagcatcg ataaagggca gcgcgtggga
     7501 ttttgcgccg aaccagacgg ttttcatgcc caatgctttt gcctgatgca ggttgtccgc
     7561 gctgtcgtcc accataatgc agcattcggg cggtacgtcc aaaaggcggc agacattgag
     7621 atacgcttgc ggattgggtt tgtacagcag tccgaaatca tccgtgccga acagtgcgtc
     7681 gaaacggttt tccaaaccga gtgcgccgac aacggcgcgg acgtaaaacg acgggccgtt
     7741 ggaaaaaact gccttgcgcc cttttaggcg gctcagggtg ttttgtgttt caggcatgcc
     7801 gtgcagccta gtcaggattg catcgatcgg atggctttcg cgcaaaaaat cggcgatgtc
     7861 gatttcggga tggtggattt gcagtccggc gagcgttgcg ccgtagcggt gccaatagtc
     7921 ttgacgcagg tcggacgcgg cagattcgga gagtttgagg cggcgtgcca tatagcgtgt
     7981 catggcgcgg ttgatgagtg tgaagaggcc ggcatcggca ttgtgcagcg tgttgtcgag
     8041 gtcgaacagc cacacggtcg ggttttcttg cattttgaac cgtgaaaatt tgttagaatg
     8101 ttattttaca gcgaatagag gaggactcgg aatgaaacgg aaaatttggc tgctgccgct
     8161 gctggcggtt tcggcatacc tgcaggcgca gacggaagtc aggctggcgg tgcataagtc
     8221 gttcagcctg cccaaagggt tgattgcgca tttcgagcgg gcaaacgatg cgaaggtgtc
     8281 gattattcag gcgggcggcg cgaacgaaat gctcaacaaa ctgattttga gccgcgccaa
     8341 cccgattgcc gacgcggtgt acggtttgga caacgccaat atcggcaagg cgcgggaaat
     8401 gggcattttg gcggcagcgc aacccgaatc cgcccccgtc gcggtcgggc tgccttcggc
     8461 tttggcggtc gattacggct atgtgtccat caattacgac aaaaaatggt ttgaaggcaa
     8521 aaagctgccc ctgccgcaaa ccctgcagga tttgacccgc cccgaatata aaaacctatt
     8581 ggtcgtgccg tcccccgcca cgtcgtcccc ggggctgggc ttcctgatgg cgaacatcag
     8641 cggtctgggc gaagaaggcg cgttcaaatg gtgggcacag atgcggcaga acggcgtgaa
     8701 ggtcgccaaa ggctggagcg aggcgtatta caccgacttt tcgcacaacg gcggcgcgta
     8761 tccgctggtg gtcggttatg ccgccagccc ggcggcggaa gtgtattttt ccaaaggcaa
     8821 atacagcgag ccgccgacgg gcaacctgtt tttaaaaggc ggcgtattcc gccaggtcga
     8881 aggcgcggcg gtcttgaagg gcgcgaaaca gccggaattg gcggcaaaac tggtgcaatg
     8941 gctgcaaagt cgggaagtgc agcaggcggt tccgtccgaa atgtgggttt accccgccgt
     9001 caaaaacacg cgcctgcccg acgtgttccg cttcgcccaa gccccgacgc acaccaccgc
     9061 ccccgcgcag cgcgatattg atgcgaacca gcgcggatgg gtttcccgtt ggattagaac
     9121 ggttttgaaa taaaacaaac ataccgcccc gcagggcttc atacggcatt tttacacctg
     9181 tgccgattac gccgcacggg gcggatgttc gatcaagagg aaaacaatgg acttcaaaca
     9241 atttgatttt ttacacctga tcagtgtttc cggttgggag catctggctg aaaaggcgtg
     9301 ggcgttcggg ctgaaccttg ccgccgcgct gcttattttt ttggtcggaa aatgggcggc
     9361 gaaacgcatt gtcgctgtga tgagggcggc gatgacgcgc gcgcaggtcg atgccacgct
     9421 gattagtttt ttgtgtaatg ttgccaatat cggcttattg attttggtga ttattgccgc
     9481 attgggcaga ttgggcgttt ccacaacatc cgtaaccgcc ttaatcggcg gcgcgggttt
     9541 ggcggtggcg ttgtccttga aagaccagct gtccaatttt gccgccggtg cgctgattat
     9601 cctgttccgc ccgttcaaag tcggcgattt tatccgcgtc ggcggttttg aaggatatgt
     9661 ccgagagatt aaaatggtgc agacttcttt gcggacgacc gacaacgaag aagtcgtgct
     9721 gcccaacagc gtggtgatgg gcaacagcat cgtcaaccgt tccacactgc cgctgtgccg
     9781 cgcccaagtg atagtcggcg tcgattacaa ctgcgatttg aaagtggcga aagaggcggt
     9841 gttgaaagcc gccgtcgaac accccttgag cgttcaaaac gaagagcggc aggccgccgc
     9901 ctacatcacc gccttgggcg acaatgccat cgaaatcaca ttatgggctt gggcaaacga
     9961 agcagaccgc tggacgctgc aatgcgactt gaacgaacaa gtggtcgaaa acctccgcaa
    10021 agtcaatatc aacatcccgt tcccgcaacg cgacatacac atcatcaatt cttaaacgcc
    10081 gtctgaaaga ggagtgggaa atggacgcgc tgcacaccat cgcccgaaac ctgacgaaaa
    10141 aacgtcaaac cgtaagctgt gccgaatcct gcacgggtgg gctgcttgcc gccgtattca
    10201 caagcgttgc aggcagttcg caatggttcg atcaaagttt tgtaacatac agcaacaaag
    10261 ccaaagaaga ccgcttgggc gtgctgcccg aaaccctgct cgaacacggc gcggtcagcc
    10321 gccaaaccgt ctatgagatg gcgcgcggcg cgaaagccgt ggcgcaggcg gattacgccg
    10381 tcggtatttc cggcatcgcc ggtccgggcg gcggcagcga aagcaaaccc gtcggcacgg
    10441 tttggttcgg gtttgccttt ccgggcggaa gttgcgaagc aatgcgccgt tttgacggca
    10501 accgcgaatc cgtccgcgcg caggcggtcg ccttcgcgtt ggaacggttg gcggggctga
    10561 ttgaaaacgg cggcgatgct gtctaaacaa aatctccgtc tggacagaat ccccgccgga
    10621 taaaaaatac cgtctgaaac gttgcaggtt tcagacggta ttttgtcggg ttaggcggcg
    10681 gtgcggctta tttcacttta ctcttcaacg cgccgtagcc tgccgcgtcc atttgttcca
    10741 gcgggatgaa tttcaagctc gcgccgttga tgcagtagcg cagtccgcct ttgtcgcgcg
    10801 ggccgtcggg gaagacgtgt cccaaatgcg aatcggcggc gtggctgcgc acttcggtgc
    10861 ggcgcatgtt gtagctgaaa tcatcgtgtt cggtaacgga ttttgcatca atcgggcgcg
    10921 tgaagctcgg ccagccgcag ccggaatcat atttgtcggc ggagctgaac aaaggttcgc
    10981 cgctgacaac gtccacataa atgccgggtt tgaacaaatg gtcgtattcg tggctgaagg
    11041 catattcggt cgcgctgttt tgggtaactt ggtattgctc ttcggtcagg gtgcgtttga
    11101 gttcggcgtc actcggtttt ttatacgttg ccgcatcgaa gcctttgcct tgcggggcgg
    11161 tcttggtttt gcccggcagc ggttcgtcgg ctttgcggat atcgatgtgg cagtagccgt
    11221 tggggttttt aatcaggtag tcctgatggt attcctcggc atcgtagaag tttttcagcg
    11281 gctcgttttc aacaacgagg ggcagttggt atttttgctg ctcgcgtttg agggcggcgg
    11341 cgatgacggc tttttcggcg gggtcggtgt agtacacgcc gctgcggtat tgcgtaccgg
    11401 tgtcgttgcc ctgtttgttg aggctggtcg gatcaacgac gcggaagaaa tattgcagga
    11461 tgtcgtctag gctgagtttg tcggcatcgt aggtcacttt gacggtttcg gcgtggcccg
    11521 tatggcggta ggacacgtct tcatagctcg gatttttcgt gttgccgttg gcgtagccgg
    11581 ataccgcgtc aaccacgccg tcgatgcgtt ggaaataggc ttccaagccc cagaagcagc
    11641 cgccggcgag gtagatggtg cgcgtgttca tgatttttga atcctttttc tgagtgtcgg
    11701 gtttgtagaa cgaatgtttc aagctgccca aatcggcatt cgggtcgcgg attaacgcca
    11761 acgcctgcgc ttcgttgatg ctgcctttga cgatgcgctg cacgtcgccg tctttaccga
    11821 ttaacgccca cgaagggtaa acgctgatat tcaggctttg ggcgatcgtg ccgccgttgt
    11881 cggttacgac gggcagcttg ggataattca aaccggcata ccatttttgg aagtcgccgt
    11941 cttttttctc gtgcaaaaag cccggagacg cgacggtaat caggttggcg gagctgaatt
    12001 ttgcatcttg cgcccatttt tcggtctgtc ccaattcgga cagacacaaa ggacaccagc
    12061 tcgcccaaaa tttaatcagc gtcggtttgt cttttttcaa gtaaacactg gcggggcggt
    12121 tgtccgcagt tttcaaagtg gataaagtgt gcggcacggt cgcggctccg gcatcgacga
    12181 ttttgggcga acaagcaccc agcgcaagca ggcagccgaa cttggcgcaa agggaaaaga
    12241 aagtacggtg tttcattttg agtttcctgt gtggacggtt tgcatgatta gacgtttgag
    12301 atgccgaaac cttacagctc ggattttcag acaaccttgc cgcgtaaaat acgctacaat
    12361 acgccctgtt tcaagtttct aaaattaaaa ggaaaattca atgttcagct tcttccgtcg
    12421 caagaaaaaa caggaaacgc cggctcccga ggaggctcaa gttcaggaaa ctgcagcaaa
    12481 agcagaatcc gaagttactc aaatagttga aaatattaaa gaagaagtcg aatctttaac
    12541 ggaaagcgtc aaagggcagg tcgaatcggc tgttgaaacc gtcagcggtg cggttgaaca
    12601 agtaaaggaa gccgttgccg agatgctgtc tgaagcagag gaagcggcgg aaaaagcagc
    12661 ggaacaagtc gaagcggcaa aagaagccat tgccgaaacc gtcggcgagg ctgtcgggca
    12721 agttcaagaa gccgttgcaa caactgaaga acacaagctc ggttgggcgg cgcgtttgaa
    12781 acaaggtttg accaaatcgc gcgacaaaat ggcgaaatcg ctggcgggcg tgttcggcgg
    12841 cggacaaatc gacgaagatt tgtacgaaga gctggaaacc gtgctgatta ccagcgatat
    12901 gggcatggaa gccaccgaat acctgatgaa agacgtgcgc gaccgcgtca gcctcaaagg
    12961 gctgaaagac ggcaacgaat tgcgcggcgc gctgaaagaa gccttgtacg acctgattaa
    13021 gcctctagag aaaccgctgg ttttgcccga aaccaaagag ccgtttgtca tcatgcttgc
    13081 tggcatcaac ggcgcgggca aaaccacatc catcggcaaa ctcgccaaat atttccaagc
    13141 gcagggcaaa tctgtgctgc ttgccgccgg cgatactttc cgcgccgccg cgcgtgagca
    13201 gcttcaagct tggggcgaac gcaacaacgt aaccgtgatt tcgcaaacca cgggcgattc
    13261 cgccgccgtg tgcttcgatg ccgtccaagc cgccaaagcg cgcggcatcg acattgtgct
    13321 ggccgacacc gccggccgcc tgcccacgca gcttcacttg atggaagaaa tcaaaaaagt
    13381 gaaacgcgtg ctgcaaaaag ccatgcccga cgcgccgcac gaaatcatcg tcgtgcttga
    13441 tgccaacatc gggcaaaacg ccgtcaacca agtcaaagcc tttgacgacg cattagggct
    13501 gaccggttta atcgtgacca aactcgacgg cacggcaaaa ggcggcatcc tcgccgcgct
    13561 tgcctccgac cgccccgtcc ccgtccgcta catcggcgtg ggcgaaggca tagacgattt
    13621 gcgcccgttt gacgcgcgcg cgtttgtgga cgcactgctg gattgagccg aaatgccgtc
    13681 cgaaaacagc agaccgatgc cgtcattccc gcgcaggcgg gaatccagac cttgggataa
    13741 cggcaatatt caaagtttat aaaagacccg tcattcccgc tcaggcggga atctagacct
    13801 tagaacaaca gtaatattca aaggttagct gaagctttag agattctgga ttcccacttt
    13861 cgtgggaatg acgggatgta ggttcgtggg aatgacgcgg tgcaggtttc cgtacggatg
    13921 gattcgtcat tcccgcgcag gcgggaatcc agaccgttgg gcatctgcag cggtttgcta
    13981 aaaaacgctt taccgtgatc agtgtgcaaa gttaaaatgg ggaagtaagc ttttcaacca
    14041 gtaattcggc gggcgcggaa tcgggcggtt taccgaaccc cggcgttcgc ggcgcgccgc
    14101 cccgtcccgt gaaggcaaac tcaaggaata aaagatgaat aaaacttgga aacggcaggt
    14161 tttccgccat accgcgcttt ataccgccat a
//
LOCUS       DAXHTY010000051        13892 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXHTY010000051 DAXHTY010000000
VERSION     DAXHTY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.051"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(69..2045)
                     /locus_tag="ACFNMK_001252"
     CDS             complement(69..2045)
                     /locus_tag="ACFNMK_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC6364525.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="ACFNMK_001253"
     CDS             2194..3033
                     /locus_tag="ACFNMK_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC6364526.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3234..4265
                     /gene="gap"
                     /locus_tag="ACFNMK_001254"
     CDS             3234..4265
                     /gene="gap"
                     /locus_tag="ACFNMK_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6364527.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKDKLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4416..5141)
                     /gene="aat"
                     /locus_tag="ACFNMK_001255"
     CDS             complement(4416..5141)
                     /gene="aat"
                     /locus_tag="ACFNMK_001255"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC6364528.1"
                     /translation="MRIPLLAPDNYAFPDPTYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(5211..5645)
                     /gene="fur"
                     /locus_tag="ACFNMK_001256"
     CDS             complement(5211..5645)
                     /gene="fur"
                     /locus_tag="ACFNMK_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC6364529.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHSEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            5864..6241
                     /locus_tag="ACFNMK_001257"
     CDS             5864..6241
                     /locus_tag="ACFNMK_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6364530.1"
                     /translation="MNKTLILALSALLGLAACSAERASLFPSYKLKVIQGNEIDPRAA
                     AALRLGMTKDQVLLLLGSPLLRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALKDRQNTDKP"
     gene            6251..7060
                     /gene="dapB"
                     /locus_tag="ACFNMK_001258"
     CDS             6251..7060
                     /gene="dapB"
                     /locus_tag="ACFNMK_001258"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC6364531.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7057..7302)
                     /locus_tag="ACFNMK_001259"
     CDS             complement(7057..7302)
                     /locus_tag="ACFNMK_001259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364532.1"
                     /translation="MPRRQLYRISFSVETPPLGHLSFGAVESDFIWEGRNPLQIRTTH
                     RATLYVSSRVLKHMPIRSLSAKCRLNNIFSDGILYGG"
     gene            complement(7320..7730)
                     /locus_tag="ACFNMK_001260"
     CDS             complement(7320..7730)
                     /locus_tag="ACFNMK_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364533.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVNCVDYPDLP
                     QEIPMPNYSDWVKIKFKQFSYLKFVYGYATKNRDKDIDNTLELGELKQDDEILDYGGA
                     LEVIGGRYDFPTAFRIDIVCREIELEFLDQEGFN"
     gene            complement(7732..7977)
                     /locus_tag="ACFNMK_001261"
     CDS             complement(7732..7977)
                     /locus_tag="ACFNMK_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364534.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNEAELARVGLRL
                     KYNGLLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(7985..9157)
                     /gene="lpxB"
                     /locus_tag="ACFNMK_001262"
     CDS             complement(7985..9157)
                     /gene="lpxB"
                     /locus_tag="ACFNMK_001262"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC6364535.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAACFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9205..10197)
                     /locus_tag="ACFNMK_001263"
     CDS             complement(9205..10197)
                     /locus_tag="ACFNMK_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6364536.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            10691..13450
                     /locus_tag="ACFNMK_001264"
     CDS             10691..13450
                     /locus_tag="ACFNMK_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC6364537.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGSDIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSENATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
BASE COUNT         3501 a         4509 c         3240 g         2642 t
ORIGIN      
        1 tgaattttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggcctgtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccatttt cgccgaatcc tgcctcatca tcactaccga caaacaaacc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccggta cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg tcagcaccac cattttcgcc cgccccaaac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagcaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccttgtgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatcc ccgccacaaa cgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaatac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccaatgc tgaacatcag gaacacaatc
     1861 ccgatttcac ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg tgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgtgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaaaac
     3061 cgtttcagac ggcattttat caagctctcc gtccgccgca cccgtgccgt ccgcatctta
     3121 ccgcccaccc ttccggccgc gccgttttca ataaaatatt aattacacgc cactacaaat
     3181 ttgctataat ccgccccgaa aatctaccca acccctcaac aaaggaacaa accatgggca
     3241 tcaaagtcgc catcaacggc tacggacgca tcggccgcca ggttttgcgc gccatctacg
     3301 attatcagat tcaagaccaa ctccaaatcg tcgccgtcaa cgccagcggc agccttgaaa
     3361 ccaacgccca cctcaccaaa ttcgacaccg tgcacggacg ctttgaagcc gacgtatccc
     3421 acgacggcgg caacctcatc gtcaacggcg acaaaatccc cttcttctcg acccgcaacc
     3481 ctgccgaact gccgtggaaa gaactcggtg tcgatttggt catggaatgc accggcgcgt
     3541 tcaccagcaa agaaaaagcc aaagttcatc tggaaagcgg cgcgaaaaaa gtcctcattt
     3601 ccgcaccggg cggcgacgat gtcgatgcaa ccgtcgtgta cggcgtgaac gacagcatcc
     3661 tgaccgccga catgaccgtc gtttccaacg cttcctgcac caccaactgc ctctcgccgg
     3721 ttgccaaagt gttgagcgaa agcgtcggca tcgtcaaagg cgcgatgacc accatccacg
     3781 cgctgaccaa cgaccaaacc gttaccgacg tgcgccacaa agacctgcgc cgcgcccgca
     3841 gcggcgtgga aaacatgatt ccgaccaaaa ctggcgcggc aaaagccgtc ggactggtac
     3901 tgcccgaatt gaaagacaaa ctcgacgggc ttgccatccg cgtgccgacc gtcaacgtat
     3961 cattggtaga tttgaacttc caagccgcgc gcgacacgac aatcgaagaa atcaacgcac
     4021 tgatgaaagc cgcctcggaa gcaggcgcgc tcaaaggcgt tttaggctac aacaccctgc
     4081 ccttggtttc catggacttc aaccacacca ccgaagccag ccacttcgac gcaacactga
     4141 ccaaagtcgt tgacggcaac atgattaaag tcttcgcatg gtatgacaac gaatggggct
     4201 tcagctgcca aatgctgaac accgcacgcc gtatgttcgg acttgaagtg cgcccgctca
     4261 aataagcaat aaaccgtcaa acaaaatgcc gtctgaaacc cgatgttttc aagttccaga
     4321 cggcattttc attttttggc agtgattttg cccggctgtc gccatttcca attttaatcc
     4381 accatatttg aacctgaccg gagcataagc cgccatcaaa gccccttgca cgccacttcg
     4441 cgccgcccga tttcctcttt caacggcacg gcgttcaaca tccgcagacg ttcggcaaaa
     4501 tccgcaaacg gcagcagctc cgaaccgaaa cggcgcatat gttccgtgtc ctgctggcag
     4561 tctatcagtt ccacacccaa atccgccaaa aacggcacgg cgcaggcaaa cgcgattttc
     4621 gacgcatccg gttgtaatgc gaacatcgat tcgccgtaaa acaccctgcc gatttgcacg
     4681 ccgtaaaagc cgcccgccaa cctcgtttca ccgctttcat cgggataatg gcactcgaac
     4741 gaatgcgcgt gtcccatttc gtgcagcttc aaatatgccg tctgaaactc gggcgcaatc
     4801 caagttccgt cctgattcgg gcgcgccgct gccgcacaat gcgcgaccac ttccgcaaaa
     4861 cagccgttga ccgcaacccg atagctgccg ttgcgcagcg ttttcgccag cgagcgcgga
     4921 acgtgcagac gttcgggaac aatcaccgca cgcgggccga ccgcatacca aaaaaaccaa
     4981 ccgtcccgag aaaaccacgg aaacacgccg ttccgatacg cctcgagcag ccgccccgca
     5041 tccaaatcgc cgctcacgcc gaccagcccg tcgcaccggg ccaaagcata ggtaggatcg
     5101 ggaaaggcat aattgtcagg ggcaagcagc ggaatacgca tggcagttct ccaaaaacac
     5161 agacggcatc caaaatcatg aatgccgtct gaacaacaaa ccgtccggat ttaacgtttg
     5221 cccttggcct gacagtcgct gcacacgccg tacatataaa gcgcgtgatc gacgatgcgg
     5281 tagccgtttt cctcagcgat tttgtcttgc agggcttcga tttcgggatt gtggaattcc
     5341 gttacctcgc cgcacttcac gcagacgatg tggtcatggt ggtcgccttt gtccaactca
     5401 taaaccgcct tgcccgtttc aaaatgatgg cgttgcagaa tacccgcctg ctcaaactgg
     5461 gtcagcacac ggtaaatcgt cgccacaccg atttccacac cctcttccaa caaaatgcgg
     5521 tacacatctt ccgcactcaa atgctcttcc gaatgcgtct cgaacaaatc caaaatcttc
     5581 aaacgcgggc cggtaacctt cagaccgctg tctttcagtt gtgcaatatt gttgaatttt
     5641 tccataatat tcaatacccc tgtaaaacaa tagacgttat aatacgcaat ttcggcctgt
     5701 ttgcccacta tcgcaccata gcagtttgca atagtaaaac cgcaccggcg gccgatgcgc
     5761 ggacagggcg gataccgctt aatcgtatga ttatcgttcg cttttataaa atattcaagc
     5821 agtgttacac tacccatccc gatttgcaca gaaaggcatt tccgtgaaca aaaccctcat
     5881 cctcgccctt tccgccctcc ttggccttgc cgcgtgcagt gccgaacgcg cctcgctgtt
     5941 cccctcatac aagctcaaag tcatacaggg caacgaaatc gacccccgcg ccgccgccgc
     6001 actccgcctc ggtatgacca aagaccaagt cctgctcctg ctcggcagcc ccctgttgcg
     6061 cgacgcgttc cacaccgacc gctgggacta taccttcaac acctcccgca acggcatcat
     6121 caaagaacgc agcaatctga ccgtctattt tgaaaacggc gtactcgtcc gcaccgaagg
     6181 cgacgccctg caaaacgctg ccgaagccct caaagaccgc caaaacacag acaaaccata
     6241 aggaacacac atgacaccgc tcaaaatcgc catcgccggc gcaaacggcc gtatgggacg
     6301 cgtattggtt gaagccgtca acaaccatcc cgacaccgtc ctttccggcg cgctcgaaca
     6361 ctcaggctca gaagccctcg gactggacgc aggctacgcc gtcggactca aaaccggcat
     6421 cgccatttca gacgacgttg acgccgttct cgcacaaagc gacgtactca tcgacttcac
     6481 ccgccccgag cccaccctca aacacctgca aaaatgcgtt gaaaaacaag tcaacatcat
     6541 catcggcaca acaggcttcg acgatgcggg caaagccgct atccacactg ccgccgaaca
     6601 aacaggcatc gttttcgccg ccaacttcag cgtcggcgtc aacctcacct tccacatcct
     6661 cgacaccgtc gcacgcgtat taaacgaagg ctacgacatc gaaatcattg aaggccacca
     6721 ccgccacaaa gtcgatgccc ccagcggcac cgcgttacgc atgggcgaag tcatcgccgg
     6781 cgcgctcggc agagacctca aacaatgcgc cgtttacggc cgcgaaggcc acaccggtcc
     6841 gcgcgatccg tcgaccatcg gctttgccac cgtccgcgca ggcgacatcg tcggcgacca
     6901 caccgccctc ttcgccaccg acggcgagcg cgtggaaatc acccacaaag ccggcagccg
     6961 catgaccttt gccgccggcg ccgtccgcgc cgcagtttgg gtaaacggca aaacgggttt
     7021 gtacgatatg caggacgtac tcgggctgaa caaccgttaa cccccataca aaatgccgtc
     7081 tgaaaagata ttgttcagac ggcattttgc cgacaggctc cgtatcggca tatgtttcaa
     7141 cacacgggac gatacataaa gcgtcgccct atgtgtcgtc ctgatttgca agggattacg
     7201 cccctcccaa ataaagtctg attctaccgc cccgaaggac agatgtccga gtggcggggt
     7261 ttcaaccgaa aaggaaatac gataaagttg cctgcgcggc ataacaagct acggctcgtt
     7321 taattgaaac cctcctgatc taaaaattct aactctattt cccggcaaac tatatctatc
     7381 ctaaaagcag tcggaaaatc atacctaccg cctatcactt ccagcgcacc tccataatcc
     7441 aagatttcat catcctgctt taattcgcca agttccaatg tattatcgat atctttatct
     7501 cggttttttg tggcgtatcc atagacaaat ttcaaatagc tgaactgctt gaatttaatt
     7561 tttacccagt ctgaataatt aggcataggg atttcttgag gtaaatccgg ataatccaca
     7621 caattgacga tgatatggaa ttcatcgttt ttcaaagtat agtttaatag agtgccctca
     7681 tatatataca gaaattgata atcttttttt ggaatggtat aagttgtcat attattttac
     7741 cttttcaaaa acaatgagtt taagattttt tgagtccaag ttggttctac tactcttatg
     7801 aaatttcagt tgtttaaact cttcctttaa cagaccatta tattttaacc ttaatcccac
     7861 tcgagccagt tctgcttcat ttggaattcc tcttaaatac ttattgtttg atgtgccatt
     7921 aatcacaatt tctccccctt tttttgtaat acgtgctgct tcgggaaaat aatccattaa
     7981 ccgctcaaca tcccgcctct tccaaaaccg cgcgcgcggc caaatccgcc gtatcttttt
     8041 tcaacagcag gtgcaacgcc ctgaaatcct gttgcagcgc ggcaacctta tcggggtgtt
     8101 cgtaccagtc cgccaacgcc gccgccagtt tttccggttt tgcttcagat tgcaataatt
     8161 ccggcacagc ctccttaccc aacaggatat tcggcaggcc gacatgcggc actttgattt
     8221 tgcgtttcac ataagcataa gtcagcggcg aaatcttgta gctgatgacc atcggacgct
     8281 tgcacaacgc cacctccaaa gtcgccgtac cgctcgttac cagcaccgca tccgccgccc
     8341 tgcacactgt ttcagactgt ctgtcgatta ccgtcagcgg caatccggca aactccggcc
     8401 gctgcaaaac ttccgccaaa cgccgcttcg tcgcctccgt tgcggcaggc agcaggaagc
     8461 atgcggcagg atagcgttcc aacaacaata atgccgtctg aaaaaacacc ggcgccatat
     8521 agtcgatttc gctgacgcgg ctgccgggca gcagggcgaa tacggggatg ccggcatcca
     8581 cgcccaaagt ttgccgcgcc gtttcacggt cgtcttccaa gggcataagc tgcgccatcg
     8641 gatgaccgac aaactccgca cgtccgcccg catcgagata aagctgcggc tccatcggga
     8701 acaggcacaa cacgcggttg acctgatgca cgattttgcc cacacgttcc cgccgccacg
     8761 cccacaccga cgggctgaca taatgcacgg tcggaatccc cgaccgtttc agcttttccg
     8821 ccacacccaa attaaaatcg ggcgcatcga taccgacaaa gacatcaggt ttcaacgaca
     8881 gcaaatcccg taccagcccc ctgcgtatcc gtaaaatttc cggcagccgc ctgaccactt
     8941 cgacaaagcc gcgcaccgcc agccgctcct gatcataaag gctctcgaaa ccttccgcct
     9001 tcatcagttc gccgccgata ccgacaaaac gcgcctgcgg acaacgcttg cggatggcgc
     9061 gtatcaggtg cgcccccaac aggtcgcccg acgcttcgcc gacactgacg gcaatcaaag
     9121 ggcttttttt atcaaccata ttcgtctgtc cccacatact ttcacgtccc gatgccgtct
     9181 gaaggcttca gacggcatcg gggttcacat tttcgtcccg ttttccaaca tcaccgcaaa
     9241 ctgtgccaag tccggcggca gctccgcctt caacaccagc ggctcgcccg tgagcggatg
     9301 gttcaagtgc agctcggacg cgtgcaaaaa catccgcttc aaacccaact tctgcaaacg
     9361 acggttcgcc tgataatcgc cgtagcgttc gtcgcccgca atcggacagc cttgagattg
     9421 caggtggacg cggatttggt gcgtacgacc cgttttcagc gttgcccgca ccaaagtcag
     9481 gtgcgacagc ccgacaccgt gcaaaatgcc gtctgaaaaa cggcttaaca cacggaacac
     9541 cgtgtgcgcc gactgcccgt cctcgctgac gcgtaccatc ttttcgcctt gtgcgccggt
     9601 atatttgaac aggggcagtt tgacatggaa attgtcgtcc ggcagtttgc ccacccccag
     9661 cgcaaggtag atttttttgg ggtggtcgtt acggatggct tcgtgaagtt tgacgagcgc
     9721 gctgcgtttc ttcgccacca tcaacaagcc gctcgtatcc ttgtccaaac gatgaaccaa
     9781 ctccaaatac ttcgcctccg gacgggcgcg gcgcaactgt tcgataacgc cgaaactcac
     9841 gccgctgccg ccgtggacgg caacgccgga cggtttgtcg atgaccaaaa gcgcatcgtc
     9901 ttcgtaaaca acgtcaaacg cacgcgccgg tacggcggca cgcctttcag acggcatttc
     9961 cttctccgcc acgcgcacag gcggaatccg caccgtatcc ccctccgcaa tacggctgtc
    10021 gggtttgcag cgtttcttgt tcaaccgcac ctcgccggcg cggataatgc ggtggatatg
    10081 gctcttggga acacccttga ggatttttat cagatagtta tcaaggcgtt gacccgcctc
    10141 atgttcggca acccctatca agctgaccga atctttgctt atttcgtgcg ttttcatcta
    10201 taatccgaac atccgtttca gcaaaaagcg cgcccgccgc gcccccctga aacggtttac
    10261 tttaaaacgg aattttatat taaaaacgca ctccgcaaag cattttccct gcgcctgttc
    10321 ccagccggca ggcgcagaac gtaatcaagt ttgaattgat tttgccgttt cggcgcggaa
    10381 gtaagacggc agccgggccc caagtcccaa acgcaaaccg cccaaaacac aggcatcacg
    10441 ataacaagaa gaagccggcc cccatttttc cgaccgcagc gttcggaacg cggcagacaa
    10501 accgccgcat cgatctttat agccttctgt atccgaccct tccgcacacg gttctccgga
    10561 acgtgcatcc ctgcggattt tcaactcaaa aactgattac cggttttatc cgaaaacatc
    10621 ggaaaaccca gccttacgga atgccgaacc ggaccggcgc gcccgaatgc cgcccgtcca
    10681 cgaggtgatc atgaaaagaa tgttattcaa cgcaacgcag gccgaagagc tgcgcgttgc
    10741 catcgtcgac ggacaaaacc tgctggattt ggacatcgaa acgctgggca aagaacagcg
    10801 caaaggcaat atctacaaag gcatcattac ccgcatcgag ccgtcgctgg aagcgtgttt
    10861 cgtggattac ggaaccgacc gccacggctt cttgccgttt aaagaagtct cccgctcata
    10921 cttccaagac tacgaaggcg gacgcgcgcg catccaagac gtgctcaaag aaggcatgga
    10981 agtcatcgtc caagtcgaaa aagacgagcg cggcaacaaa ggcgcggcgc tgaccacctt
    11041 catcagcctc gccggacgct atctggtatt gatgccgaac aacccgcgcg gcggcggcgt
    11101 atcccgccgt atcgaaggtg aagagcgtca agagttgaaa gccgccatgg cggaactcga
    11161 cattccgaac ggcatgagca tcatcgcccg taccgccggc atcggccgca gcgcggaaga
    11221 gttggaatgg gatttgaact atctcaaaca actctggcag gcgattgaag aagcaggaaa
    11281 agcgcatcac gacccttacc tgctctttat ggaaagctcg ctgctcatcc gcgccatccg
    11341 cgactatttc cgccccgaca tcggcgaaat tctggtggac aatcaagaag tttacgacca
    11401 agttgccgag ttcatgagct acgtcatgcc gggcaatata ggccgtctga aactctacga
    11461 agaccacacg ccgctgtttt cccgcttcca aatcgaacac caaatcgaaa gcgcgttttc
    11521 gcgcagcgtc agcctgccct ccggcggcgc gatcgtcatc gaccataccg aagccctcgt
    11581 ctccatcgac gttaactctg cacgtgccac acgcggatct gacattgaag acactgcgtt
    11641 caaaaccaat atggaggccg ccgaagaagt cgcccgacaa atgcgcctgc gcgacttggg
    11701 cggcttggtc gtcatcgact tcatcgacat ggaaaacccc aaacaccagc gcgatgtgga
    11761 aaacgtcctg cgcgacgcgc tcaaaaaaga ccgcgcccgc gtacagatgg gcaaactctc
    11821 ccgtttcgga cttttagaat tgagccgcca acgtttgaaa ccggctttgg gcgaaagcag
    11881 ccacgtcgcc tgtccgcgct gcgccggcac cggcgtgatt cggggcatcg aatccaccgc
    11941 cctgcacgtt ttacgcatca ttcaagaaga agcgatgaag gacaacaccg gagaagtgcg
    12001 cgcccaagtg cccgtcgatg tcgccacctt cctgctgaac gaaaaacgcg ccgagctgtt
    12061 tgcgatggaa gagcgtttgg atgtcaacgt cgtcctgatt ccgaacatcc acctcgaaaa
    12121 tccgcactac gaaatcaacc gcatccgcac cgacgacgta gaagaagacg gcgaaccgag
    12181 ctacaaacgc gtcgccgagc ctgaagaaga cgaatccgcc aaaccgttcg gcggcgaaaa
    12241 agccaaagcc gcccgtcccg aacccgccgt caaaggcgtg cgccacacca gccccgcccc
    12301 gactgccgcc cccgagaaaa aaacctcttg gtgggacagc ttcaaagctt ggctcaaacg
    12361 cattttcggc ggcagcgaaa cccaagccgc gcccgctgcc gaaacctccg aaaaacgcag
    12421 cacggcaaac cgcagcggca gccgcgccaa caaccgccgc caaaacccgc gccgcagcaa
    12481 acgcgaaggc agcaaagtag aagtccgcga agtggcaggc aaaactgccg gacaggaagc
    12541 gcgtgccgac aaagccgaaa cgcgcaacaa cggcaaccgc cgccgcaacg aacgcggcga
    12601 ccgtgccgcc ggacgcgccg acgaagcgga aatccaagac cgcaacatac agcctgccgc
    12661 aaccgttgca gatgccgcac cgtccgaaac cgaagtgcaa accggaaaac gccgccgcaa
    12721 cggcagccgc agcgaacgcg gccaaaccgc gccggaaacc gccaccgttg ccgaaacaac
    12781 cgttcagaca gcggaaaaca cgccgtccga accgcatacc gcagaagaca aaggcagcaa
    12841 gcccaaatcc gaacgcaacc gccgcgaacg cgacagccga gatgccaaag aacgccgcga
    12901 gcgcaacaat cagcgcgacc gccgtcaaaa cggcaaaaaa cgcaatattc cgtctgccgc
    12961 caaaatcgag cagtacctga atattcacga caccgccgac aaagtccgtt ccgccgccgc
    13021 gcacgttttc ggcgaaaccg acgcaaacgc gccgattacc gtcagcattg ccgatccggt
    13081 tgcagaaagg gatcttccga cagcatctcc cgccgtttca aacggcgacg caccggttta
    13141 tgatgcggcg gaaaaaatcc gccgtgccac cgccgccatc ctgcccgaag gcgcgacacc
    13201 gaaagccgaa gcacaggaaa tgccgtctga aaacgcaacc tttacggctg cggcggaaca
    13261 ggcacgggaa accgcacaaa ccggcggact cgtcctgatc gaaaccgacc ctgccgcatt
    13321 gaaggcatgg gcggcacaac ccgaagtcca agccggacgc ggtttgcgcc gttccgaaca
    13381 gcccaagccg tctgaagccg caaccgtccc tgccgaagaa atgatccaag tcgaaacccg
    13441 gcaaggctga accgacggcg gcaaaaagag gttctgttcc gcagaacctc ttttttacat
    13501 gggttcggat acctgcaatg ccgtctgaaa cttcgccatt cccgtgatta ccgaaacatt
    13561 ccgccattcc catgattccc gcaacattcc gtcattccca tgattcccgc aacattccgt
    13621 cattcccatg aaaacgggaa tctagaacct caaactttcg gataatcttt gaatattgcc
    13681 gtcgcccaaa ggcctggatt cccgcctgcg cgggaatgac ggcggagggt ggacgatgcc
    13741 gtctgaaact tcgccattcc catgattacc gcaacctttc gtcattcccg ccacctttcg
    13801 tcattcccgt gaaaacggga atctagaacc tcaaactttc ggataatctt tgaatattgc
    13861 cgccgcccaa aggtctggat tcccacaaca tt
//
LOCUS       DAXHTY010000052        13497 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXHTY010000052 DAXHTY010000000
VERSION     DAXHTY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.052"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(78..278)
                     /locus_tag="ACFNMK_001265"
     CDS             complement(78..278)
                     /locus_tag="ACFNMK_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364538.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            428..1567
                     /locus_tag="ACFNMK_001266"
     CDS             428..1567
                     /locus_tag="ACFNMK_001266"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC6364539.1"
                     /translation="MSQNASVGIVMPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIEQWAAIVGGSLGGMQA
                     LQWTISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGFGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGDSLTRALQNVKAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            1564..2145
                     /gene="metW"
                     /locus_tag="ACFNMK_001267"
     CDS             1564..2145
                     /gene="metW"
                     /locus_tag="ACFNMK_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC6364540.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTNSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            2262..3107
                     /locus_tag="ACFNMK_001268"
     CDS             2262..3107
                     /locus_tag="ACFNMK_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
                     /protein_id="HFC6364541.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(3157..3717)
                     /gene="efp"
                     /locus_tag="ACFNMK_001269"
     CDS             complement(3157..3717)
                     /gene="efp"
                     /locus_tag="ACFNMK_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC6364542.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(3760..4908)
                     /gene="earP"
                     /locus_tag="ACFNMK_001270"
     CDS             complement(3760..4908)
                     /gene="earP"
                     /locus_tag="ACFNMK_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC6364543.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRDA
                     VRFDTEALRERLMLPEKNASEWLLFGYRSDVWAKWLEMWQQAGSPLTLLLAGAQIIDS
                     LKQSGIIPQNALQNDGDVFQTASVCLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETASAHRRLSDDLNGGGALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(4927..5205)
                     /locus_tag="ACFNMK_001271"
     CDS             complement(4927..5205)
                     /locus_tag="ACFNMK_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364544.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
     gene            5385..6326
                     /gene="miaA"
                     /locus_tag="ACFNMK_001272"
     CDS             5385..6326
                     /gene="miaA"
                     /locus_tag="ACFNMK_001272"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC6364545.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            6330..7049
                     /gene="tadA"
                     /locus_tag="ACFNMK_001273"
     CDS             6330..7049
                     /gene="tadA"
                     /locus_tag="ACFNMK_001273"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC6364546.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSCHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            7054..7542
                     /locus_tag="ACFNMK_001274"
     CDS             7054..7542
                     /locus_tag="ACFNMK_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC6364547.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGILGVKYTGAGRLAVRGAFIGSIIGMFFSLPGL
                     ILGPFIGAAAGELIDRRNMLLAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIVY
                     LF"
     gene            7609..8361
                     /gene="rlmB"
                     /locus_tag="ACFNMK_001275"
     CDS             7609..8361
                     /gene="rlmB"
                     /locus_tag="ACFNMK_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC6364548.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFVDTTRLDAISKGARHQGVAGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGESDLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(8423..9814)
                     /locus_tag="ACFNMK_001276"
     CDS             complement(8423..9814)
                     /locus_tag="ACFNMK_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFC6364549.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(9829..10083)
                     /locus_tag="ACFNMK_001277"
                     /pseudo
     CDS             complement(9829..10083)
                     /locus_tag="ACFNMK_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10121..10996
                     /gene="dapA"
                     /locus_tag="ACFNMK_001278"
     CDS             10121..10996
                     /gene="dapA"
                     /locus_tag="ACFNMK_001278"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC6364550.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLTEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQVDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            11005..12201
                     /gene="bamC"
                     /locus_tag="ACFNMK_001279"
     CDS             11005..12201
                     /gene="bamC"
                     /locus_tag="ACFNMK_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC6364551.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTLIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKISQDSIRRLFDTVGLGGIYSTGERDKFIIRIEQGQNGVSDIFFAHK
                     AMKEVYGGKNKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPILPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAITE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYTEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(12258..13190)
                     /gene="pip"
                     /locus_tag="ACFNMK_001280"
     CDS             complement(12258..13190)
                     /gene="pip"
                     /locus_tag="ACFNMK_001280"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC6364552.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIRKWL
                     VFGGSWGSTLSPAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT         3444 a         4052 c         3296 g         2705 t
ORIGIN      
        1 cacctcctac cgcaagccct tttcatcaaa tatgccgtct gaaacagaaa aatgcgtttc
       61 agacggcatt ttttccgtca atacgaatcc ggaatgtgtt tgaaatacag cataaacaga
      121 taccaaaaca gcatcgcggc ggctgcaccg agcagtaccc atgcgctgat ttcggcgact
      181 tcgcgcaggg cgcgtttttg cctgcggctg aaaaggcggt tgataatcaa cgcggcaagt
      241 gcgaaaaaga aaaaaatacg gaaaagtctg cctatcatgt cgggaagcgg tggaggtcgt
      301 ctgaaaaagt atgattcgtg tgcggcggat gagggtcaaa cgcgtccggc tgcttgtccg
      361 accgtgtcaa aaagtcatgc cgtttgcgat ataataacgc ttttcgccgc atttgttgga
      421 acaggatatg agtcaaaatg cctcggtggg cattgtaatg ccccaaaaaa ttccgtttga
      481 gatgccgctg gttttggaaa acggtaaaac tttgccgcgt ttcgatctga tgattgaaac
      541 ctacggcgag ctgaatgctg aaaaaaacaa tgcggtttta atctgccacg cgctgtcggg
      601 caaccatcat gttgcgggca ggcattcggc ggaagataaa tatacgggct ggtgggacaa
      661 tatggtcgga cctggcaaac cgattgatac ggaacgtttt ttcgtggtcg gtttgaataa
      721 tctgggcggc tgcgacggca gcagcgggcc tttgtcgatt aatcctgaaa cgggcaggga
      781 atacggcgcg gattttccgg tggttacggt gaaggactgg gtaaaatccc aagccgcgct
      841 tgccgactat ctcggtatcg aacaatgggc ggcgattgtc ggtggcagct tgggcggcat
      901 gcaggctttg cagtggacga tttcctaccc cgagcgtgtg cgccatgctt tggtgattgc
      961 gtctgcgccg aaactgtcca cgcaaaatat cgcgtttaac gatgtggcgc gtcaggcaat
     1021 tttgaccgac cctgatttca atgaaggaca ttaccgcagc cacaataccg ttcctgcacg
     1081 gggcttgcgg attgcccgca tgatgggaca catcacttat cttgccgaag acggtttagg
     1141 caaaaaattc ggacgcgatt tgcgttccaa cggttatcaa tacggctttg gtgttgaatt
     1201 tgaagtggaa tcctatcttc gctatcaggg cgacaaattc gtcggacggt tcgacgccaa
     1261 cacttacctg ctcatgacca aggctttgga ctatttcgat ccggcggcag atttcggcga
     1321 cagcctgacc cgcgccctgc aaaatgtgaa agcgaaattc tttgtcgcca gcttcagcac
     1381 cgactggcgt ttcgcacccg aacgttcgca cgaactggtc aaggcactga ttgccgccca
     1441 aaaatccgtg cagtatatcg aagtcaaatc cgcacacggg cacgatgcct ttttaatgga
     1501 agacgaagcc tatatgcgtg cggtcgccgc ctatatgaac aacgtttata aggaatgtca
     1561 gcaatgaacc tgcgcgatga tttgcaactg atttacgacc ggatacccga aggcagccgc
     1621 gtcttggact tgggctgcgg cgacggcgaa ctgcttgccg cattggtcga acacaaaaaa
     1681 tgcagcggct acggcatcga aatcgacaca aatagcgtca tcgccgcgat gtcgcgcggc
     1741 gtaaatgtta tccaagccga tttggaagaa ggtttaaccg cattcaacga tcaaagtttt
     1801 gatgtgattg tgttgagcca aaccatccaa gcgatgcaga ataccgaaaa aatcctgcgc
     1861 tgcctgatgc gcgttgccaa acaggcgatt gtcagcttcc cgaatttcgg ctactggcgc
     1921 aaccgcgtcc aaatcgcact cggcggacat atgccggttt ccgaacgtat gccctaccat
     1981 tggtacgaca cgcccaatat ccattggtgc acgctcaaag actttgattt gttgtgcgcc
     2041 aaaaacaaca tccgcgtgct tgagcgcgcc gtgatgacgg gcaacagaca ggtcaaacat
     2101 ttccccaacc ttttgggcag ccttgcattt taccgcgtcg ggtaagacag attgagaacc
     2161 ttgtcggcat taacaggttc ttcggttcta ttaacaacag gccgtctgaa aaatgaaacg
     2221 gtaggttttc agacggcctc tttaatactg gaaacaatag aatgaaaaac aaaacctcat
     2281 cacttctctt atggcttgcc gcaatgatgc tgaccgcgtg ttccccgagc aaagaagata
     2341 aaacgaaaga aaacggcgca tccgccgcct cgtccacggc atccgccgct tcgtcttccg
     2401 caccccaaac cgatttgcaa ccggccgcat ccgcccctga taacgtcaag caggcagaaa
     2461 gcgtgccgcc gtcaaattgc accgacctgc accccgccac cggcattgac gatctcatgc
     2521 agcaaatcgc cgaacacatt gactcggact gtctgtttgc cctttcccat cacgaactgg
     2581 aaacccgttt cggcttaccc ggcggcggct atgacaacat acagcggctg ctgtttcccg
     2641 acatccgccc tgaagatccc gactaccatc agaaaatcat actggcaatt gaagacttgc
     2701 gttacggaaa gcgcacgatc agccggcagg cacaagatgc cttgatggaa caggaacgcc
     2761 gcctccgaga agcgacgctg ttgctgatac agggcagtca agaaacccgc ggacaaggcg
     2821 aggagccgaa acgcacgcgt tattttgaag tttcggcaac ccctgcctat tcgagccggc
     2881 acaacaacgg acttggcggc aatttccaat acatcggcca attgcccggc tatctgaaaa
     2941 tacacggaga aatgcttgaa aaccaatcac tcttccggct gtccaaccgt gaacgcaatc
     3001 ccgacaaacc gtttttagac atccattttg acgaaaatgg caaaatcacg cgtattgtcg
     3061 tttacgaaaa aaacatctac ttcaatccaa acttggggcg aagataaaaa aatgccgtct
     3121 gaaacgtttt cagacggcat ttttcaatgc aacaaatcaa gcgcgtttgc ggaattcgcc
     3181 ggtgcgggta tcgatttcga ctttgtcgcc gttttcgatg taagacatca cttggatttc
     3241 ggtgccgccg accaggcgcg cggttttcat cactttgccg gaagtgtcgc ctttgacggc
     3301 aggctcggtg tactcgactt cgcgcacgat gatggtgggc agttcgacag aaatggggtt
     3361 gccttcgtag aaggtaactt cgcattggtc ttccataccg tcaacgatga atttcaacgc
     3421 gtcgccgatg ttgtcggctt cgatttcgta ttggttgaat tcttcatcca taaagacgta
     3481 catcgggtcg gcaaagtagc tgtacgtaca gtttttgcgg gacaggatga ccacgtcgaa
     3541 tttgtcgtcg gctttgtaaa tggtttcgga agccgcgccg gtcagcaggt ttttcagttt
     3601 catgctgact ttggcggaag agcggccgcc tttgatgtat tcggtttttt gaacgaccat
     3661 aggatcgttg ccgaccataa atacattgcc ggcgcgcagt tcttgtgctg ttttcattgt
     3721 gattttccaa tcaaatcggt tgtcgtaaaa cgcgctattc tagcgtattt tttgatgctt
     3781 tgaaacaaag gcggcgagtt tttcggatgc ggaaggctgc ccgaaaagat aacggctcca
     3841 atcttccgcg ccttgccgcc agccgttttg atgttgttgc agggtttgcc aacattcgag
     3901 gcgttgcgtg gcggataaag cccctccgcc gttgaggtcg tctgaaaggc ggcggtgtgc
     3961 cgatgcggtt tcgggcgtgt agaaaccgtg tgccttatcc caaaaggcgt ggagtttgtc
     4021 gagatggaca ttctcatctt gcgggtagat gtgccaaaag aagggtttgc ccgcaagctg
     4081 ggcgcgcacg aaactgtctt cgccgcggat gacggcgcaa tcggcgaggt gcagcagttt
     4141 gtcgaagtcc tgttgcggca cgaaagggat tttgacaagg cagacggatg ccgtctgaaa
     4201 aacatcgccg tcgttttgca gggcgttttg cggaatgatg ccgctttgtt tgaggctgtc
     4261 gataatttgc gcccccgcca gcaacagtgt caacggactg cctgcctgtt gccacatttc
     4321 cagccacttt gcccaaacat cgctccgata gccgaaaagc agccattcgg aggcgttttt
     4381 ttcgggcagc atcagccgct ctcgcagggc ttcagtatcg aaacggacgg catctcggta
     4441 atcacgttcg cgtatcaacc cgccgctttt ttcgctgaaa cccataaacc aaaaatattt
     4501 ttgaacaccc tcctgcggcg aaggcatcag gtgcagcctt tcattgcttt cctccgcgct
     4561 caaatattcc caattcagcc aaagcggctt gtgtcggcgg ataatgtgca gcacattttc
     4621 gggcaggtcg caggcaaaag tttcgatgac ggcatcggga acaggcgcgg tatcaatatc
     4681 tgccgcatcg gaatgccaag tgcggacatg aatatcctga tgaacgcagg gaacatcggg
     4741 caaatcaggg caaagcgcac gcaaggcgga cacatcgtcc gtccacaaat gcacctgcca
     4801 accgagttcg cggtgcaaaa cacgggcaag ccgccacgaa acgccgatgt cgccgaaatt
     4861 gtcgatgacc ttgcaaaaaa tccagcagac aaaaggagga gtattcatag aaataacgga
     4921 aacgggctaa aacgcgccaa aatagtggaa caaatacaca atcagcaaaa taatcggaat
     4981 aaacagcaac atagtttgac gtttcaaatc aaactcttgt ttcaaatgat gcttgatggt
     5041 gtcgaaaagg acgaatttaa acaaaaacgt cgcttcgcag gcaaggatga tgccgttaat
     5101 cagatgctgg ggtttgtctt cgggcggatt ggcgacgaaa acggcaagcg tcgccaaggg
     5161 tatcaggcag acgacaagcc agcggacaag caggtttcgg gacattgcaa ggctttcaaa
     5221 aaagcaaaac gccattatcg ccccaagcgc acgccttgtc taacggatgc gccggagatg
     5281 ccgtctgaag ccttgccgcc ggaaaacgca gtgcttccgc aaaccaaaat ataacgtcgg
     5341 cacgctttcc gctataatcc gcattttcgc aaccctgaaa catcatgccg accccgaaag
     5401 cctttgccct gctcgggccg accgccggag gcaaaaccgc gctcgccctc aaaattgccg
     5461 aaaccctgcc tgtcgaaatc atcagcctcg attccgcgct ggtttaccgc gatatggaca
     5521 tcggcacggc gaaaccgtcc gcctccgaac gcgcctttgt cccgcaccac cttatcgaca
     5581 tcatcccgcc taccgaatca tacagcgccg cccgctttgt cgaagactgc acgcgcttgg
     5641 tcggggaaat ttcctcacgc ggcaggtttg cccttattgt cggcggcacg atgatgtatt
     5701 tccgcgccct gacccaaggt ttgaacgatt tgcccgaagc cgacgcctgc ctgcgtgccg
     5761 acttggacga acaaaaacag atgtacggct tggatttcct ctaccgcacc ctgcaaaaag
     5821 tcgaccctga aaccgcatgc cgtctgaaac cgaacgacag ccagcgtatc gaacgcgctt
     5881 tggaagttta ttatctgacc ggcaaaccga tgagcgaaca cctcggcaga caaagccccc
     5941 atacgctccc cttcgatttg cataccgccg ccctgattcc cgaaaaccgc gcccgcctgc
     6001 atgaaaatat cgccctgcgt ttccacctga tgcttgaaca gggctttatc ggcgaagtag
     6061 aaaacctgcg ccgccgctat cccggcctga ccgccgactc ccccgccatc cgctgcgtcg
     6121 gctaccgtca ggcatggaaa tatctggatg gaaaaaccga tttcccggca tttgtcgaga
     6181 aaggcattgc cgccacccgc caacttgcca aacgccaact gacttggttg cgcaaaacac
     6241 ctttagactg cgttgccgac ccgttttcag acggcacttc atgcacacgc ctgattgagg
     6301 cggcaaaacg gtttttcggc gcataaacca tgctgaccac accgccgctc gcccccaaaa
     6361 ccgtcgccgc cctgcacagg ctcggtatcc gtacgctcga agaactgcgg caaaacggtt
     6421 cggtcaaagc ctttctgctg ctcaaagcat cgggtttgac cctcacgaaa agcacgctgt
     6481 ggcagcttga gtcgctgctg aacggcacac tcccgcaaga aatcacgcaa cacgacaaaa
     6541 cgcgcctttt tgccgaattg aaaaaccatc cgcccgtcgc cgcctttccg ccgcaggaag
     6601 aaatggaaca cttcatgcgc gaagcactgc gccaagcaga acaatccgcc gctgacggcg
     6661 aaatacccgt cggggcagtc atcgtttcag acggcaaaat catcgcatcg gcacacaaca
     6721 cctgcattgc cgactgcaat gtcagctgcc acgccgaaat caacgccttg gcacaggcag
     6781 gcagcgaaat gcaaaactac cgccttgacg gatgcgacat ctacatcacc ctcgaaccct
     6841 gcgccatgtg cgcgtccgca ctgatacagg cacgaatcag gcgcgtgatc tacggggcgg
     6901 cagaacccaa aaccggcgcg gcaggcagca tcgtcaacct gtttgccgac aaacgcctca
     6961 atacgcacac cgccatacgg ggcggaatcc tgcaagaaga atgccgcgcc gtgttaagcc
     7021 gtttttttca aaacaaaaga aaaggttgaa cctatgaccg tactgaccgt catcctcgcc
     7081 ctcgccctga tagccgtcgg cacggcgggc atcgtttacc ccgccctgcc cggattggca
     7141 ttgatgtttg ccggaacatg gctgcttgcc tatgccggcg gctaccaaat ctacggcgcg
     7201 ggcgttttgt ggacggtcgg actcatcagc cttgccggca tactggcgga ctatgtggca
     7261 ggcatattgg gggtaaaata taccggagca ggcaggctcg ccgttcgcgg tgcttttatc
     7321 ggcagcatca tcggtatgtt tttctccctg cccggactaa tactcggccc ctttatcggc
     7381 gcggcggcag gcgaactcat cgacagacgc aatatgcttc tggcaggtaa agcgggcttg
     7441 ggtacgctgt tgggacttgt cgtcggcacg gcgttcaaaa tcggctgcgc cgtatccatc
     7501 ttgtttatcc tgttggtgaa atacatcgtc tacctgtttt aaccccttca gacggcatac
     7561 cggcaaattg ctataatgcc gtctgaaccc ttttcacaaa gcaccatcat ggcaaaccaa
     7621 agacccatct acggcttcca cgccgtcaat gcccgtttgt ggcaaaaccc caaatccatc
     7681 gtcgaactct acatccaaga aggcaaatca gacgcacgca cgcgcgaagt gttggaaaag
     7741 gcggcaaacg aaaacatccg cgtatatttt gttgacacca cccgtcttga tgccatcagc
     7801 aaaggcgcgc gccatcaggg tgtggccgga tttatcgatg cctccaaaaa ccacgtccat
     7861 ctcgaagacg tattggaaaa cttaagcgaa ccgccgctgc tgctgatact cgacggcatc
     7921 accgatccgc acaacctcgg cgcgtgcctg cgtaccgccg acgcaatggg cgtacacgcc
     7981 gtcatcgcac cgaaagacaa aagcgcgggg ctgaacgcca ccgtcagcaa agtcgcctgc
     8041 ggtgcggcgg aaaccgtccc ctacatcacc gtaaccaacc tcgcccgcac cctacgcgaa
     8101 ttgaaagaat acggcatttg gatcatcggc accgatatgg gcggcgagtc cgacctttac
     8161 cattgcgatc tgcccgacag cgcggcgtgg gtaatgggca acgaaggcga cggtatgcgc
     8221 cgcctcacgc gcgaacattg cgacatgctg gtgtccatac ccatgttcgg cacggtcgaa
     8281 agcatgaacg tctccgtgtc tgccggaatg gtattaagcg aaacccgccg acaacgcgta
     8341 ttaaaaaacg aaaaagcgta agtctcaaaa acaatgccgt ctgaaacagg gattctgttt
     8401 cagacggcat tcattttcaa aatcagtgtt ccaagtggtc ggtatcaaac ttgaccgccg
     8461 cctcggtttt atcttcgggc aagaccaaat tcagcaagac tgccgtaatg ccgccggcgg
     8521 aaatagagtt ttggaacaag acaggcaggt ttttaaacac ttccggctca aacgccacgc
     8581 ccaagcccaa gccgaccgaa gtcgccgcaa tcaccgcctc gcgcctgcgg atgccgtgac
     8641 tgaccaaaat ccgcacgccc gcaatcgcaa ttaagccgaa catcaaaacc atcgcgccgc
     8701 ccaacaccgg actcggaatc gtcgtaaacg cgcgaccgac aacggggaac agacccaaca
     8761 gcaccaaaat cacggcaata tatttgccca catgacgcga agccacgccg gtcatctgaa
     8821 tcacgccgtt gttttgcgca aacgtcgtca gcggcagcga acccaaagcc gtcgcaatca
     8881 ccgacaccaa gccgtcagcc aacacgccgc cgcgcaaacg tttggtgtat tcctcgcctt
     8941 caatcggctg gtcggacacc attgccgtcg cggttaaatc gccgaccgcc tcaaacacgc
     9001 tcaacaagaa aatcgcgccc gccacaataa acgcgtgcca gtcaaaagca aaaccatatt
     9061 taaacggtac gggcagcgta accagcggca ggttttgcag cgcggaaaaa tccaccttgc
     9121 ccaaaaacag cgcgacgata tagccggcaa tcagcccgac cgcaatgccg ctcatgcgca
     9181 acagcgggtt tttcatgcag ttgaacacca acacaatcag caacaccagc gatgccagcc
     9241 ccaagttttc catcgagccg aacgtgccgt ccgccttcgc gccgaagccg ccgccgaaat
     9301 cggtaatacc gacgtgtacc aaactcaagc cgatcagcat caccaccaca ccgctgaccg
     9361 tcggcgtaat cacctttttc aaatacggca gaagccacgc cgagaaacac accaaaaacg
     9421 cgccgacaaa cgatacgccc aagagcgtcg aaatcatcgc atccttagtc aaaccgccct
     9481 ctttcatccc cgcgcccagc gcaatcatga cggtaacgaa cgagaaattc accgactgga
     9541 tagacagcat ccccgaaccg accggcccga agcggttgac ctgcaaataa gtgccgacac
     9601 ccgacgcaac catcgccatc gacacgagat acgccgtcat ctccaccggc agttccagcg
     9661 cgccgcccac aatcagcgcg ggcgtaatca tcggcacgaa aatcgccaaa agatgggtaa
     9721 ccgcgctcaa cagcgcatta ccgaacggcg gcctatcctc caaaccgtac accaaatcag
     9781 gcgcatccgc cccttttccc atatgaccgc tcataatgtt tccttgcttc aaatgttgaa
     9841 aaaaccaaag cgcggattgt agcgaatccg ccataccgct tcaatttcga tttcggacgg
     9901 catacgcgcc cgtccggcaa acagattccg aaccgtcgcc gccggaatat cgtttacaaa
     9961 acgcaataac ctgatttcaa acacaaaaat acaaacccga cgcatccgcc gtcaagccgc
    10021 cccgctcatc ccggacccgc cggatttcac acctgcggta aagtaggcta caatcggaag
    10081 cattaccgcc ccctacacca caactgataa aaggttctgt atgttacaag gtagcctggt
    10141 tgccctgatt accccgatga atcaagacgg cagcatccat tacgaacaac tccgcgactt
    10201 aatcgactgg cacattgaaa acggcacgga cggcatcgtt gccgtcggca cgacaggcga
    10261 atccgccacc ctctccgtcg aagaacacac cgccgtcatc gaagccgtcg tcaaacacgt
    10321 tgccaaacgc gtccccgtca tcgccggcac aggggcaaac aacaccgtcg aagccatcgc
    10381 cctttcccaa gccgccgaaa aagccggggc ggactacacc ctctccgtcg tcccctatta
    10441 caacaaacct tcgcaagaag gcatgtaccg ccatttcaaa gccgttgccg aagccgccgc
    10501 gattccgatg atactctaca acgtacccgg cagaaccgtc gtcagcatga acaacgaaac
    10561 catcctgcgc cttaccgaaa tccccaacat cgtcggcgtg aaagaagcca gcggcaacat
    10621 cggcagcaac atcgaactca tcaaccgcgc gcccgaaggc ttcgtcgtcc tttccggcga
    10681 cgaccatacc gccctgccgt tcatgctgtg cggcggacac ggcgtgatta ccgtagccgc
    10741 caacgccgcg cccaagctct ttgccgatat gtgccgcgcc gcgctgcaag tcgacattgc
    10801 cctcgcacgc gaactcaaca accggctgat tccgatttac gacaccatgt tctgcgaacc
    10861 cagccccgcc gcgccgaaat gggcggtatc cgccttgggc agatgcgaac cgcacgtccg
    10921 cctgccgctc gttccgctga cggaaggcgg acaggcaaaa gtccgcgccg ccctgaaagc
    10981 ctcaggacaa ctctaaatat aactatgccg tctgaaccgt tcggacggca taacgcaaca
    11041 aacactttaa tatccatcac acaggatgac acgatgaccc ttatcaaacc cgtcattgcc
    11101 gcgctcgcac tcatcgggct tgccgcctgc tccggcagca aaaccgaaca gcccaagctc
    11161 gactaccaaa gccggtcgca ccgcctgatc aaactcgaag tcccgcccga tttgaacaat
    11221 cccgaccaag gcaacctcta ccgcctgcct gccggttcgg gcgccgtccg cgccagcgat
    11281 ttggaaaaac gccgcacacc cgccgtccaa cagcctgccg atgccgaggt attgaaaagc
    11341 gtcaaaggtg tccgcctcga gcgcgacggc agccaacgct ggcttgtcgt tgacggcaaa
    11401 tcccctgccg aaatctggcc gcttctgaaa gccttttggc aggaaaacgg cttcgacatc
    11461 aagtccgaag aacctgccat cggacaaatg gaaaccgagt gggcggaaaa ccgcgccaaa
    11521 atctcgcaag acagcatccg ccgcctcttc gacacagtcg gcttgggcgg catctactcc
    11581 accggcgaac gcgacaagtt catcatccgc atcgaacagg gtcaaaacgg cgtttccgac
    11641 atcttcttcg cccacaaagc catgaaagaa gtgtacggcg gcaaaaacaa agacacgacc
    11701 gtatggcagc cctccccgtc cgatcccaac ctcgaagccg ctttcctgac gcgctttatg
    11761 caatatttgg gcgttgacgg acagcaggcg gaaaacgcat cggcaaaaaa accgatcctt
    11821 cccgccgcca acgaaatggc gcgtatcgaa ggcaaaagcc tgattgtctt tggcgactac
    11881 ggcagaaact ggcggcgcac cgcgctcgcc ctcgaccgca tcgggctgac cgtcgtcggt
    11941 caaaacaccg aacgccacgc cttcctggtt caaaaagccc cgaacgaaag caatgcaatt
    12001 accgaacaaa aacccggcct gttcaaacgc ctgctgggca aaggcaaagc ggagaaacct
    12061 gccgaacagc cggaactgat tgtctatacc gagcctgtcg ccaacggctc gcgcatcgtc
    12121 ctgctcaaca aagacggcag cgcatatgcc ggcaaagacg catccgcatt attgggcaaa
    12181 ctccattccg aactgcgtta aaccattcaa aacaaaagca gcttccaaac agaagctgct
    12241 tttttatgcg gaacttttta caacaaatgg ggcaaaatat cctcaactgc ctgaaccaac
    12301 gcatccacca aaggcggatc gaacgcacga tgccctgcct gaaccaccct caattccgct
    12361 tcgggaaagg ctttcgacag cgcccacgca ctctgcatcg gcgtacacaa atcataccgc
    12421 ccctgcacga taacggtcgg gatatgccgt attttgccga tattgttcaa aatcgcctta
    12481 tcgccctgca accagccgcc gttgacaaaa taatggtttt ccaaacgcgc gattgccagc
    12541 gaggcataag catcttcatc cacttcctcc ggctcgaaac ggatcagata gctttcccaa
    12601 tccgcccaag cctttgcggc agacaggcac acttcttcat cttgatgaaa cagcaacccg
    12661 tgatacgcct caatcagccg gttccgccga ttttcagcaa tcggcgcgac aaatttttgc
    12721 cattgttccg gataaatccg gctcacaccg cccgcctcgt tcagccacgc cgtttcagac
    12781 ggcctgcaca aaaatatccc gcgcaatacc aatcccttta cccgttcagg atgggtttgg
    12841 gcataagccg gcgacaaagt gctgccccac gaaccgccga acaccagcca tttcctgata
    12901 cccagcattt cacggacttt ttcaatatcc gccaccaaat cccaagtcgt attgtcttcc
    12961 gcacaagcat acgggcgcga acgtccgcaa ccgcgctggt cgatgatgac gatgcggaac
    13021 acatcgggat tgaaaaaacc ccgacattca ggcgaagccc ccgcgcccgg cccgccatgt
    13081 aaaaaaataa ccggcacacc gtcgggattg cccgattcct cccaataaat ttgatgaatt
    13141 tcagacacct gcaagtatcc gctatgaaaa ggctgtttta tctcatacat catcttctcc
    13201 catattgctt ctgacaatcc gaacattccc acacgcaaca tcacgcacga gacacaagcc
    13261 gccccgaaag gcaaatgccg aaaaatcgcg atatagtgaa ttaacaaaaa tcaggacaag
    13321 gcggcgagcc cgcagacagt acggatagta cggaaccgat tcactcgtgc ttaagcacct
    13381 tagagaatca ttctctttga gctaaggcga ggcaacgccg taccggtttt tgttaatcca
    13441 ctataacagc aaccctgtcg ccgtcattcc cgcaaaagcg ggaatccagt ccgttca
//
LOCUS       DAXHTY010000053        13450 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXHTY010000053 DAXHTY010000000
VERSION     DAXHTY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.053"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(77..883)
                     /locus_tag="ACFNMK_001281"
     CDS             complement(77..883)
                     /locus_tag="ACFNMK_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC6364553.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILNR"
     gene            1103..2473
                     /gene="ffh"
                     /locus_tag="ACFNMK_001282"
     CDS             1103..2473
                     /gene="ffh"
                     /locus_tag="ACFNMK_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC6364554.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFINNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLHKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMRGIK
                     GMFPGL"
     gene            complement(2807..3502)
                     /locus_tag="ACFNMK_001283"
     CDS             complement(2807..3502)
                     /locus_tag="ACFNMK_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6364555.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADIDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARSAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(3673..4332)
                     /locus_tag="ACFNMK_001284"
     CDS             complement(3673..4332)
                     /locus_tag="ACFNMK_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFC6364556.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMGDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRRNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(4535..6645)
                     /locus_tag="ACFNMK_001285"
                     /pseudo
     CDS             complement(4535..6645)
                     /locus_tag="ACFNMK_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(6831..7436)
                     /locus_tag="ACFNMK_001286"
     CDS             complement(6831..7436)
                     /locus_tag="ACFNMK_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6364557.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAAVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(7485..7883)
                     /locus_tag="ACFNMK_001287"
     CDS             complement(7485..7883)
                     /locus_tag="ACFNMK_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6364558.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            7991..8902
                     /locus_tag="ACFNMK_001288"
     CDS             7991..8902
                     /locus_tag="ACFNMK_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6364559.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            8892..10193
                     /locus_tag="ACFNMK_001289"
     CDS             8892..10193
                     /locus_tag="ACFNMK_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC6364560.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(10261..10431)
                     /locus_tag="ACFNMK_001290"
     CDS             complement(10261..10431)
                     /locus_tag="ACFNMK_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364561.1"
                     /translation="MLLYRLLGGAEIECGRCPVPPMTDWQHFLPAMGTVSAWVAVIWA
                     YLIIESEKSGRY"
     gene            10588..12738
                     /gene="dinG"
                     /locus_tag="ACFNMK_001291"
     CDS             10588..12738
                     /gene="dinG"
                     /locus_tag="ACFNMK_001291"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC6364562.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQNNAQQNLLGFEAPEVLWD
                     SKPKPEELKLLHDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAVAVRSLLKHVIGLNDALS
                     AARREKEQDGALLDRLTGDFGIFIARIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPGEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRVKTQRYGQQLLAGLPPFKRIG"
     gene            12774..13388
                     /locus_tag="ACFNMK_001292"
     CDS             12774..13388
                     /locus_tag="ACFNMK_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC6364563.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGAPVAGAASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIATAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT         3284 a         3755 c         3420 g         2991 t
ORIGIN      
        1 ccctcctctg actggtgcgc ctttgtgaat atgccgtctg aaactcgggg actcagacgg
       61 catctgttgg ctcttcttat ctgttcagaa tgatttccaa tacgaacttg ctgcccatat
      121 aggcaatcat caggctgaca aatccgatga tggtccacac ggcggctttt ttgccgcgcc
      181 atgcggtcat gctgtgcttg agcagcagtc cgccgtaaat cagccatgac aatatgccga
      241 atacggtttt atgggtaaag gtcatgggtt tgccgaatac ggcttcggca aaaaatgttc
      301 cgctgacgac ggaataggtc agcaggatga aacctgccca catggcctgg aacatgagtt
      361 tttccaaact gagcagcgac ggcaggaatc ctgcgagctt ggagaagctc ctgcggtgca
      421 ggctgcgatt cagcagcagg gtcaaaacgg acaataatgt tgcgatgccg aacagcccgt
      481 atgcaagcag cgaagttccg atatgcagca taaagggaag gtcggtaatt tcatatcccg
      541 agaattttcc aggaaaaacc aaacctgaca gcagcatcag tgcggcgcaa ggatacagca
      601 gcaactgcac tccgcgcagc ggataaaaga agctgccggc aaaataaata aacagcatca
      661 tccaaacaat caggctgccg gaatacccga agcccataat gatgattttg tcttgaatga
      721 ccggcatcag cagtgccgcg ccgtggacgg tcaatgccgc gcccaaaacc ggcaattccg
      781 tcttccacgg gtaatcccga ccgcgcccct gctgttggca gtgccatgca aatgcaccca
      841 atcctgcgta aaccgccgtc aaaaagatga aaactgtcgg catggtggac tttctctatc
      901 tatactgttg cgccgtctgc ggccgcttat gaaatattgg aacttttaac gttggaattg
      961 taaaatcccc gtttcgggca agccttgacg gatttgccga tatgctgtcc ggcacacaag
     1021 ccgcatcaaa ttattttgat tttattttaa caaagaatgc ccctgatggg gcaagctatt
     1081 cttattcaga ccaaggacca gtatgttaga caatttaacc ggccgcttca gcaatgtctt
     1141 caaaaacatc cgggggcagg ccaaactgac cgaagacaat attaaagagg ccttgcgcga
     1201 agtccgcctc gccctgcttg aggcggatgt cgccctgcct gtcgtcaaag agttcatcaa
     1261 caacgtcaaa gaaaaggccc tcggtcagga agtagcgggc agcctgacgc cggatcaggc
     1321 atttatcggc gtggtcaaca aagccctgac cgaactgatg ggcagggaaa acaaaacgct
     1381 ggatttgtcg gttgcgccgc ccgccgtcgt gttgatggca ggtttgcagg gcgcaggcaa
     1441 gacgacgacc gttggcaaac tcgcccgcct gttgaaaaac gatcagaaga aaaaggtttt
     1501 ggtggtatcc gccgacgttt accgtcctgc cgcgattgaa cagctgcgtc tgttggccga
     1561 acaggtcggc gtggattttt tcccgtccga taccaaccaa aaaccggttg aaattgcaac
     1621 tgccgccgtc gattacgcca aaaaacattt ttacgatgta ttgatggtcg ataccgccgg
     1681 ccgtttggca atcgatgaag agatgatgaa cgaaatcaaa gcccttcacg cggcggttaa
     1741 cccggtggaa actttgttcg tcatcgatgc gatgctgggt caggatgcgg tgaacactgc
     1801 tcaggcattt aatgaagccc tgccgctgac cggtgtcgta ttgaccaaga tggacggcga
     1861 ttcgcgcggc ggcgcagctt tgtccgtgcg ccacgtaacc ggcaaaccga ttaaatttat
     1921 cggtgtcggc gaaaaaatca acggcctcga acctttccac cccgaccgtc ttgccagccg
     1981 tatcctcggc atgggcgacg tattgaccct gattgaagac gttcaaaaag gtattgatga
     2041 agaagccgcc gccaaaatgg cgaaaaagct gcacaaaggc aaaggcttcg acctcaacga
     2101 ctttaaagaa caaatccagc aaatgcgcaa tatgggcggt ttggaaaacc tgatgtcgaa
     2161 aatgccgggc gaactgggtc aaatctcgaa acaaatcccc gaaggaacgg ctgaaaaagc
     2221 gatgggcaaa gtagaagcca tcatcaactc gatgacccct aaagaacgcg ccaaccctgc
     2281 cctgctcaaa gccagccgca aacgccgtat tgcaatgggt gcgggcacaa ccgtgcagga
     2341 agtgaacaaa ttgctcaaac agtttgaaca aatgcaacag atgatgaaga tgttcagcgg
     2401 caacggcttg ggcaaactga tgcgtatggc gaaaggaatg agggggataa aagggatgtt
     2461 cccgggtttg taagccgatt taacagaaaa cgccgtctga aaagtttcag acggcgtttt
     2521 tgttttatat tctgatttat agtggattaa caaaaatcag gacaaggcgg cgagccacag
     2581 acagtacaga tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct
     2641 ctttgagcta aggcgagcca acgccgtacc ggtttaaatt taatccacta tatattctga
     2701 tttaaaacca taaggcttta agcaatcatc tcttctataa agcctaaata caaaaggccg
     2761 tctgaaatcc tattttcaga cggcctttac tcattcaatc ctcaacttat tgcggctttt
     2821 tctgctcttc gcgtacttta tccaccaact ggtcaatcgt ggtcatacca gactgccagt
     2881 ctttaaattc aacttggtat ttgccgccga cgatcacagt cggtgtgccg ctgatttgga
     2941 atttattggt caactcttcc atttgagccg aacgcgcttg gctttcagga gcctcaaatg
     3001 cagccaatac ttttttgccg tcaaacgctg tttgctcgga cagccatttt ttcagggtat
     3061 cgatatcagc cagattgatt ttttgattaa ccatcgcatc gaaaatatgg ctgttggctt
     3121 tatctgattc accggccatt tccactgcgg ccgccaaacg tgccaaaggt ttcatttcat
     3181 caccccacac gacatgctcc cggcgcatat aggtatcgtc tttaaacgtt ttgatgtgct
     3241 cgctcaagac cggctcaaga tgggcgcaat gcgggcagaa gtagccgaaa aattccaata
     3301 cttcgatttt accggcctgc tgttgcggaa taggcgtaga caatacagtg tagttcacac
     3361 cttcgttcaa ctcagcaggg gctgccggag cagatgagct gctttggacg ctgtctgccg
     3421 gaacgctggt ttcagcctgt ttgctacaag cggccaacgc caacagggtc aatgaagtca
     3481 aagctaaggt tttcagtttc ataggtatct cttgtgtgtc agattaatgt gcggatttta
     3541 tgacatttta ttgaaggcgt gttcgatttg agtgaaaaaa tgtgtttagt tttatttcag
     3601 cctttataaa ggccatctga aatatggctt agataattag ccttattttc agacggcctt
     3661 tgttctagta atttacggtt tcggacattt tgtcttcaat ccgtcgatgt gttccattaa
     3721 gtcaggtggg acatgattcc acataggatc aagaataaaa tcgatacctt cccgtctggc
     3781 taatttggct gctgggacaa aatcggaatc accagaaaac aagatgattt tatcggcttg
     3841 tttttttaag gtaatagatg aaatatccaa gcctatacgc atatctacac ctttttgttt
     3901 aacatcgagc acgacatcgt tttccgtaat ttgagttgac gggtttttca gaagattttt
     3961 catgacttgg ggttttaata tccatgctgt attgtccgac agcttgccta aacgtaacgc
     4021 taatttacgc ttcttcttca attcggtatg cagattgcga cgaaatatgg cttccggagt
     4081 ctttgaaaaa tctatggcac gatgagatac gggattgtgc attttctttt caaacggcac
     4141 gcagtcgtaa aagaaaattc tatataaatc acccatttga tctgtgccgt tgattttatc
     4201 tttcagatgc agcatcgaat atcggaaagc caaatcagca attctttttg catcgtgtgg
     4261 attttgagac aggcctaatg accgtacccg cttaataaaa aatgcaccgt caatcaaaat
     4321 ggcggttttc atattgcttc ccctatattt gtcaaagata taaaaaagcc cttgggatcg
     4381 gcatacccgc tatgatttaa tgggtaatgc gtactgccaa gggcactatt tctttttgga
     4441 ttatactgat gtagataaag cctgtcaaaa agttcgtatg ctgaatttac ggattatcag
     4501 gattttcaga cggcattctg ttgcacgaac gggattagaa actgtaatta acccgcgccg
     4561 tataagtgcg cggcgcgccg ggcatggcat ccgaacgcca atatttttga ttgagcagat
     4621 tggctgcggc aaaggtaatg ttaacatttt tatggttcca gccaagcatg gcatcaactc
     4681 gggcaaagcc tggaagcgta gtcacttctt tatttcttga gttgtaaccg tagcgtttgc
     4741 ctgtaccggt tacgccgatt tcgccgtaga ggttttcggt cggggtataa cggaaaaaca
     4801 ggttgccggt aacgttgctg gtgttattca aatggatgcc cactcggtcg ggattttttt
     4861 gtcttcaacg actttcgcct gcatcacgcc caacgaaccg cgcagataga gttttttagg
     4921 gatgatttgc ccgatggcgg acaattccac gccgcgcgaa cggtgtttac cgccgactgc
     4981 ccaagtgtag ggatcatttt gctcgtcggg gcggtagcgg atattgaagc gttcgatttg
     5041 gtaggcggac aatgtggtgc tgaggcggtc gtccagccaa ctgcttttga cgccggtttc
     5101 gtattggcgg gtgtactcgg ggtcggcgtt gaacacggcg gaagacgacg tgttgatgct
     5161 caaatagccg ccgcgtccgc cataaggcgc gaagcctttg ttatacgagg cgtaaagtgt
     5221 gtggacggga ttgatgttcc acactgcacc gatgttgggg ctgaacgagt gtccgctgta
     5281 ctggcggctg ctgccggtga gtttgttttc ggaattaaag gtgtacttgt cgtaacgacc
     5341 gccgaggacg aatttcaaat cgggcgtggc ggagaagatg ttttgcacga agatgccgta
     5401 ggagtcggct ttgtggcggt tttgggtcag aataggctgc aatctgcccg aagccggcca
     5461 gcttgctcgg tcgtatggat cgatggaggc ggtaaagttg cgtctgaaac ccaatgtcgg
     5521 gttgcggtgt tcgcggctgt aatccatgcc tacggtcagg tggttttcaa aacggccgat
     5581 ggtgtagtcg ccgttgagcg tgaagttgga cgacagggtt ttgttgtcgg tctgctgcca
     5641 ggcgtagtta cgtttgatta agttgccatt ttcgctgcct gcatagaaat gatcaaaatc
     5701 ctgcgccgcc gtgcggtggg cgagctgcca ttgggcacgc catttgtcgt tgaaggcgta
     5761 ttcaaggtcg gaacgccaaa cttgcagctt gtctttgaca aaatcgttcc ggtgggcgaa
     5821 ccccatgcgg taaggcagtc cgaagcggtc gtacacggac ttggtcggac tgcggtcggg
     5881 cgtgcgctcc acattgtcgt aggtgtattg ccccgtccat ttcaagccgt tgtcgagttt
     5941 gacggtaatg ctgggtgaaa ccatgacatt tttgctgtct atgccgctgc ggaacgaatt
     6001 ggcgcgcccg acttcgccgg tgagacggat ggcgacgttt ttgttcagca cttcgttaat
     6061 gtccatattc aggctgcggt ttgcccacga accgtaaacc gcaccgatgt tgcggctttg
     6121 tttgaagttg gcgtatttgc tgaccatgtt gatgacgccg ccgccgttgg tgcggccgta
     6181 aagcacggaa gacgggcctt tcaggatttc cacgcgctcg atgttggcag tactgcggcg
     6241 cacttgtccg ctttcgcgca cgccgtcgcg gtaaatatcg gatgcatcgg cttgaaaacc
     6301 gcgcaggaaa atgctttcgc cgcgcatatc gtaggcagcg tcgatgccgg cattgccttc
     6361 gaggatggaa ctcaaatcgt tcgtaccgta atttttgttt ttctggatat tgagcgtatc
     6421 gatggtttgc ggcgtttctt tgatgagctg tccgttgcgg gtaacggcgg cttcgtcgta
     6481 gttgatgtag cctttgagta cgctggtgtc ggactgtccg accacggaaa cggtaggtag
     6541 cgtggcggtg taatgttcac cattgtcctg cgtatcggcg gcagcaacag ggaaggaagc
     6601 aataatcagc gtgggtaata aagctaaatg aaatgatatt ttcattttta tactcaattt
     6661 aacaaaacaa ccgaattata ttgcctcacg gaggaaatga gaataatttc ttttaactat
     6721 attgaacatg atatttgtaa acaaaggtct cagaatgcgg aaaactcgcc gcctgatact
     6781 gaaaaatgcc gtctgaacag ggttcagacg gcattttttt gaccgcgaaa ttatgcgccg
     6841 aacactttca aacgttctgc aactggttca aaggtctttt cacctgccgc cccttcaata
     6901 ccgccgataa ccatttgcgc gcgcaacaac cagttttcgg ggatattcca cgctttggca
     6961 atcgccgcat cgggcaaggg attgtaatgt tgcaggtttg cacctacgcc gaccgcggca
     7021 agtgtcgtcc aaacggcata ctgcaccatc gcgttcgcct ggtccgccca aacgggaaag
     7081 ttggcggcat aagcagggaa ctgctcctgc aaacctttga cgacattttg atcttcataa
     7141 aacaaaatgg ttgccgcacc cgccttaaac aggttcaatt tttgcgcggt cggttcaaaa
     7201 ctgtcggcag gcacggcggc acgcagcgcg tcttcgacaa attgccacac cttatcatgc
     7261 tcttcgccaa acagcacgac cacgcgggca gattgggaat tgaacgaaga aggtgtgtgc
     7321 aaaacggcgt gttcgacgat ttggacaact tcatctttgc cgacgggcag atttttattt
     7381 aacgaataaa tggaacggcg gctttcggca gcctgttgca gagattgacg ggtcatttct
     7441 tttcctcaaa aacattcaac aaaatacact ttaggcagtc cgattcaggc tttacgcttg
     7501 aaaaacagcc tgtccagcga ccatgctccg ccgcccgccg ccgcgatata gaggaatacg
     7561 aagcagaaca gcactgcgga ctcgccgccg ttggcaatcg ggaacaaagc atttccggaa
     7621 gcgtgcgcca taaaataggc aaccgccatc tggccggaca aaacaaacgc ggcagggcgc
     7681 gcaaacaggc ccaacaccag caaaatgccg ccgacaattt ctaaaatacc ggcaagcagc
     7741 aacagcccgc cgggcgaacc gctgcccatt tcaatgggga aggcgaagat tttcgacgta
     7801 ccgtgcaaca aaaacaggta ggcggttacg atacgcaaaa cagaaagcaa aaccggttgg
     7861 atacggttgc agcaatcgga cataaaagct cctttgatat ttgtcttcat ttcaggcggt
     7921 agagtataga tgtttcccct tttcaaatat atactgcttt tattgataca acttttccac
     7981 acaagaaaat atggacaccc tgttcagcct caaggttttc cgccaagtcg tccaaagcgg
     8041 cggcttcacc cgcgccgccg acgcgctcgg catctccacc gcaatggcaa gcaaacacgt
     8101 cagccacttg gaaaacaccg tccaagccaa actcctgcac cgcaacagcc gcaacctcag
     8161 cctgaccgaa gccggggaag aatactaccg gcaatgcagt tacgcgctcg acacgctcga
     8221 cgatgccgcg caaaaagccg ccggggggac ggaaaaaccg caggggctgc tgcgcgtaac
     8281 gatgccactg tggtttgccg gcagccagat atgcaactgg ctggcggaat accgcgaacg
     8341 ttatcccgaa gtgacattag aactgatttt ggacaaccgc cacgtcgatt tgattgccga
     8401 aggcgtggat ttggcgttgc gcgtttccca aaccctgtcc ccgtcactga ttgcgcgccc
     8461 actggcggaa atcgaatttg ccctgcttgc ctcgcccgac ttcctgaggc gcaacggcgt
     8521 gccggaaacg ccggaagaag tggcggggct gcccgccgtc ctgccgacct acaccaacca
     8581 gcagaaactc gacctcaccc gcaaatcgga cggcaaaaaa taccggcttg aactgacccc
     8641 cgtcatccgt accgacaaca cgctgatgat gcgcgaaatg attaaggcgg gcgcgtgcat
     8701 cggttatcag ccgctttggg cggcggaaca cgatttgcgc tgcggcacgc tggtgaggct
     8761 gctgcccgga tacgccgtcc cgaccgaccg gctgaatgcc gtttatgcag acagggcatt
     8821 cttaagcgcg aaagtccgca gcttcatcga ttttctgaac gaaaaaatcg ccagcaggaa
     8881 aggctgccga aatgccgtct gaaaccgccc gcccccttta tgcggacacg tgcgccgcac
     8941 acacgctcgt ctggttccgc caaaacctcc gcatccgcga caacgccgcc ttatgcaccg
     9001 ccgttgccga aggtttgccc gttatcggta tttggattga cgatgccgaa acagacaacc
     9061 ctcgccgcgc cgcgttctac cgccaatccg ccgccgaact cgcccaaggg cttgcagggc
     9121 gcggcatccc gctctacacg gcggcatcgc ctgccgagct cgtccggctc gccgtccgcc
     9181 tcaatatccg cgccgtcatc gccgacgaat cccatacttt tgccgacaaa ctcgccgaca
     9241 acgccctttg gcatgaattg gacaaacacg gcatcgcgtt aaccttcgtc aatgaccgcg
     9301 ccgttttcgg caaaaccgac ctgatacccg acggcggcac ggcacacacc gatttcgacc
     9361 gctaccgcga agtatggctc gaccgctttt ccaagcagcc ccccgccggt ccggacctat
     9421 tcgcggcata ccgccaaccc ttccccgaaa acctttccgc cccgccgcct gccgcccttt
     9481 cagacggcat cttcctgccg caaaacagcg gcgaaacggc ggcttggcgg cagtggcggc
     9541 ggtttctcga acaggcggat tcctactccg ttttaaagga tttcccctcg cgcaaacaca
     9601 cttcgctgat gggcgcgtat ttgagtgccg gctgcatctc gccgcgcctg ctcgcgcggg
     9661 aaagcctcga acgccgtctg aacgcgtggg cggacaacat catccgccgc gattttttcc
     9721 ttcaacttgc cttgcagcac acggatgacg acccttcaga cggcaatcct gaacacaccc
     9781 tgcgcctgac gctttggcag cagggccgga ccggcattcc gattatcgat gccgcgatgc
     9841 gctgtttgca caaaaccggc agcctccacc ccgccctgag acgcttgagc gcggattttt
     9901 tctgccacgt tttaaacctc ccccgccgcg aaggcgagat atggtttgcc cggcagctga
     9961 ccgatttcga tgcggcaatc aaccaaggca actggtggct tgccgcctca cggcacacct
    10021 gccccgacat tgccgccgcc tcataccgta ccgacccgga cggtactttt atcaaacggc
    10081 acattcccga actcgcccac ctgtccgccg acaccgtcca cacgccttgg cggtttgcct
    10141 gttcggtcga tacccacggc tatcccgccc atcctgtcgc cggtattccc taagcggtac
    10201 ggcaacctaa aacaaatgcc gtctaaactt ccgtttcaga cggcattgcg tccgaatgac
    10261 tcaatatctt ccgctttttt cactttcaat aatcaggtat gcccaaatca ccgccaccca
    10321 agccgacacc gttcccatcg ccggcaaaaa atgctgccaa tccgtcatcg gcggcacagg
    10381 gcaacggccg cattcgattt ccgcgccgcc caacaaccgg tacagcaaca tatccgcaat
    10441 ccaaagcaac aacgggtatt tcaaacgctg caaccaaaaa accaaccacg tccgcacgat
    10501 tcacctcacc ccccattccg acaacgatat tgtaccgctt ccgtgcaatg ttaaaataag
    10561 cccacttcta ccgccgtaca aaacgccatg ctcaccgatt tagaaaaaaa cgccatccgc
    10621 gaccattacc aaaacatcgg caaaaacctg cccggtttcc gtccgcgcgc ttcgcagcgg
    10681 gaaatgattg cggcggttgc caacgctttt tcgcggacgt tggcgcgcga agaaggcggc
    10741 gagccgccca agcgcgaggg cgagagcatc gccgtaatcg aagggccgac cggcgtgggc
    10801 aaatcgcttg cctacctgct ggcgggcggc atcatggcgc agacgcgcgg caagcggctg
    10861 attgtgagca gcgcaacggt tgccttacag gagcagttgg ttgaccgcga cctgccgttt
    10921 ctggtcgaaa aaagcggttt ggaactgacc ttcgcgcttg ccaaagggcg cggccgctat
    10981 ctctgccctt acaaactcta ccaactgacg caaaacaacg cccagcaaaa cctgcttggt
    11041 tttgaagcac cggaagtatt gtgggacagc aaacccaagc ccgaagaatt gaagctgctg
    11101 cacgacattg ccgacgaatt ttccgcccga cggttcaacg gcgaccgcga cacttggccg
    11161 gaaaaaatcg atgacgcgat ttggctcaaa gttaccaacg accgccacgg ctgcctgaaa
    11221 accgcctgtc ccaaccgtcc ggaatgtccg ttttacctgg cacgcgatgt cttggaaacc
    11281 gtcgatgtcg tcgttgccaa ccacgacctt ctgcttgccg acatcagtat gggcggcggc
    11341 gtgattctgc ccgcgcccga aaacagtttc tattgcatag acgaagcgca ccacctgccc
    11401 aaaaaagccc tcagccgttt tgccgccgaa cattcgtgga atattgccgt ttggacgctg
    11461 gaaaaactgc cgcagctgac cggcaagatt gccgcgctta ccgataaagc cgaacttgcc
    11521 aaccttgccg acgaagccgc cgcatccttg ctcgacagcc tgcacgaatg gcagttccat
    11581 ttggcggaag agccgtcttt gagcttgggg ccgtctgaaa acgacaggcg aaccaacagc
    11641 gaaccgactt ggctgtggga agacggcaaa atccccgaag gcctcgaaac caccgtttcc
    11701 aacaccgccg ttgctgtgcg cagcctgctc aaacacgtta tcgggctgaa cgatgcgctt
    11761 tctgccgcac gccgcgaaaa agaacaggac ggcgcgctcc tcgaccgcct gaccggcgat
    11821 ttcggcatct tcatcgcccg tatcgaacaa atcagcgcgg tttgggattt gctctccacc
    11881 gtccccctcg aaggcgaaga gccgctggca aaatggataa cccgccgcgc cgacgacaaa
    11941 aacgactaca ttttcaacgc cagccccatc agcagcgcgt cccacctcgc caacagcctg
    12001 tggcggcgcg cggcaggcgc ggtattgacc tccgccaccc tgcaatcctt gggcaacttt
    12061 aacctgatgc tgcgccaaac cgggctgcaa tggctgcccg agaccaccac cctcgccctc
    12121 aaaagcccct ttgactttga aaaacagggc gaactctaca tcccccccat atacgccagc
    12181 cccaaagacc ccgaagccca caccgccgcc atcatcgagt ggctgcccaa gctcatttcg
    12241 cccggcgaag ccatcggcac gctcgtcttg ttttcctcgc gcaaacaaat gcaggatgtc
    12301 gccctgcgcc tgcccggaga ctacctgccg ctcttgctcg tacaaggcga attacccaaa
    12361 gccgttctcc tgcaaaaaca ccaccgggcc atagaagaag gcaaagccag catcatcttc
    12421 ggactcgaca gctttgccga aggactcgac ctgcccggca ccgcctgcgt gcaagtcatc
    12481 atcgccaaac ttcccttcgc catgcccgac aaccccatcg aaaaaaccca aaaccgctgg
    12541 atagaacagc gcggcggcaa ccctttcatc gaaatcaccg tccccgaagc cggcatcaaa
    12601 ctcatccagg ccgtcggccg cctcatccgc accgaacaag actacggccg cgtaaccatc
    12661 ctcgacaacc gcgtcaaaac acagcggtac ggccaacaat tattagccgg cctgccaccg
    12721 tttaaaagga tagggtaaac ctaccgccct gcccgcaaca gaaaggaaaa atcatgaact
    12781 tcacccgact gctcaaccaa gtcttaagca cggttcaaaa aaaaggcaac accttctccg
    12841 acagcccgct caattcattc ggcggaggcg cgccggttgc cggtgccgcg tccatgctgc
    12901 tgaacggtaa aaaccgcaaa accattacca aaatcggttc gaccgccgct ttgggctacc
    12961 tcgcctaccg gggctatcag atgtggcagc aaaacaaagg gcgggcaacc gtaacacaaa
    13021 gcgatttcca acctgccgga gaaactgaag aaacatacag ccgcaccgta ttgcgcacca
    13081 tgatagccgc cgccgcttca gacggcatga tagacgaagc cgaacgccgg actatcgaac
    13141 aggaaagcgg cacagacccc gaaactgccg catggctcgc cgccgaatac cgcctgcccg
    13201 caagcatcgg ggacatcgcc accgccgtcg gcaacgatga ggcgttggcg gcggaggcct
    13261 atctggcggc aaggttggtc tgtgccgatt tgtcgcggaa agaaaccgtc ttcctcgccc
    13321 gcctgtcgca ggctttgaaa ctggatgaca atctggtgga gagtttggaa aggcaattgg
    13381 ggttttgatg acacagccag ggagggaaaa tcatcaaaaa acggttattc cgaccccggc
    13441 ctcaaaacgg
//
LOCUS       DAXHTY010000054        13303 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXHTY010000054 DAXHTY010000000
VERSION     DAXHTY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.054"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(214..723)
                     /locus_tag="ACFNMK_001293"
     CDS             complement(214..723)
                     /locus_tag="ACFNMK_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC6364564.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(1055..1387)
                     /locus_tag="ACFNMK_001294"
     CDS             complement(1055..1387)
                     /locus_tag="ACFNMK_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364565.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDMPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            complement(1432..1539)
                     /locus_tag="ACFNMK_001295"
                     /pseudo
     CDS             complement(1432..1539)
                     /locus_tag="ACFNMK_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1678..2514
                     /gene="cysT"
                     /locus_tag="ACFNMK_001296"
     CDS             1678..2514
                     /gene="cysT"
                     /locus_tag="ACFNMK_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC6364566.1"
                     /translation="MLLLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            2769..3629
                     /gene="cysW"
                     /locus_tag="ACFNMK_001297"
     CDS             2769..3629
                     /gene="cysW"
                     /locus_tag="ACFNMK_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC6364567.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLILVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNTV"
     gene            3626..4699
                     /locus_tag="ACFNMK_001298"
     CDS             3626..4699
                     /locus_tag="ACFNMK_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC6364568.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYSGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KALKLIPKQVYVFSQNELIEYSI"
     gene            complement(4756..6282)
                     /gene="ilvA"
                     /locus_tag="ACFNMK_001299"
     CDS             complement(4756..6282)
                     /gene="ilvA"
                     /locus_tag="ACFNMK_001299"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC6364569.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSHGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGDETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            6430..7599
                     /locus_tag="ACFNMK_001300"
     CDS             6430..7599
                     /locus_tag="ACFNMK_001300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC6364570.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNRPTVHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVGTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYTGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKTAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWMRLTGQ"
     gene            complement(7724..8296)
                     /locus_tag="ACFNMK_001301"
     CDS             complement(7724..8296)
                     /locus_tag="ACFNMK_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC6364571.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTTVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(8363..9346)
                     /locus_tag="ACFNMK_001302"
     CDS             complement(8363..9346)
                     /locus_tag="ACFNMK_001302"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC6364572.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(9513..9588)
                     /locus_tag="ACFNMK_001303"
     tRNA            complement(9513..9588)
                     /locus_tag="ACFNMK_001303"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9553..9555),aa:Gln,seq:ttg)
     gene            complement(9597..9672)
                     /locus_tag="ACFNMK_001304"
     tRNA            complement(9597..9672)
                     /locus_tag="ACFNMK_001304"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9637..9639),aa:Gln,seq:ttg)
     gene            complement(9685..9760)
                     /locus_tag="ACFNMK_001305"
     tRNA            complement(9685..9760)
                     /locus_tag="ACFNMK_001305"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9725..9727),aa:Gln,seq:ttg)
     gene            complement(9768..10613)
                     /gene="ispE"
                     /locus_tag="ACFNMK_001306"
     CDS             complement(9768..10613)
                     /gene="ispE"
                     /locus_tag="ACFNMK_001306"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC6364573.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKLRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHESLTRDSASSIMPTFQNLRPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            complement(10623..11204)
                     /locus_tag="ACFNMK_001307"
     CDS             complement(10623..11204)
                     /locus_tag="ACFNMK_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC6364574.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            complement(11344..13188)
                     /locus_tag="ACFNMK_001308"
     CDS             complement(11344..13188)
                     /locus_tag="ACFNMK_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6364575.1"
                     /translation="MLPNRFKMLTVLTATLIAGQVSAAGGGAGDMKQPKEVVKVFRKQ
                     QRYSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPE
                     VAERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLE
                     EVLAQSDDVQKRRIFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSV
                     QGREKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQ
                     EMEIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAY
                     GRGTEEQRSRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGG
                     RAALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGS
                     NTELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLD
                     EGFALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGE
                     VLWALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
BASE COUNT         3242 a         3697 c         3147 g         3217 t
ORIGIN      
        1 atgtaggttt tcttaaccct gcgtcctaga ttcccgcttt cgcgggaatg acgaaaagtg
       61 gcgggaatga cggttcgggc attccttaaa tcacccgtgt atcgctgtaa atcttagaga
      121 tggcggaata tagcggatta acaaaaacca gtacggcaag gcgaggcaac gccgtactgg
      181 tttttgttaa tccgctataa ttgatgaaac gggttaaaaa agtgttgcca tcgcctgttc
      241 ttcttgtcgg atacgcttaa ataagaccag caaataaatg ggcaggccaa tcaataaaac
      301 ataccacgct tgacataata aggcgatgcc aatcagttcg ggtatgatgt ttaaaaaata
      361 attggggtgg cggaatgttt taaacaacca cgaacgatta atttgatgat ttggtaaaat
      421 atagatttta accgtccaaa tctcccccaa ctgcttaata atcaatgaca atatcacaaa
      481 cgaagccatc accgtcagcg taccaatcaa ggatatgcca ttaaaagcag tgtcagaaag
      541 ccatacccaa acaaaacacg ccaaataata aagcgtatga actgccgcaa gcagcgtgga
      601 attggttttt ccgtattgtt tcgccccttt ggcaatcaag gctttttcat gtttaataga
      661 gacggctaaa aataacagtc tgatgataaa aaacaggctt aaaatgctta aaatcattgt
      721 cattgatgtt tttccattga aattgaaata aatataaatc ggattaatgg tatttttaat
      781 taatgatgtt tcagaccatc atgctctata aacaattccc attaagtccg cgccgcaacc
      841 tgctataata agtctgcaat cggcgcaaat caatgctttg cgtttattgc catcccaaaa
      901 taattgatgc tgccttaatt ataataccaa gataagtttt tttattcaat aaaatacaaa
      961 gggaagcgtt cagcccattt caaacagatg caatccaccg attatttaaa aaacggcaaa
     1021 gccttgcccc ccttgcggca agcctgcaat gcctttaatg tccgcaggcg caagcgtcgc
     1081 cgtggtgccc gcaaccctgt gccgtttcga gccattcgtc gtcatcgccg tcaaactctt
     1141 ccacttcgtc aaactcgccg cgttccacca tgacgcacaa ctcttcaagg ctttccgcgc
     1201 cttccaccca aaaacaggga atgcccggcg gcatatcggg cgcaagcagt gccgctacgc
     1261 cttcatgcca cgcaatccaa tagccgttgt ccgtacggac gaattgtgcg ttttcgttga
     1321 ccgactcgcc ttccgtacct tccagcatac gttccgcaat gtccggttga aaagataaaa
     1381 tctgcataag tgttccttta tatgatggtt ttccgtcaaa acaaggtgtt atagtggatt
     1441 aaatttaaac cagtacggcg ttgcctcgcc ttgccgtact atttgtactg tctgcggctt
     1501 cgtcgccttg tcctgattta aatttaatcc actatatttt aaaacatcgc gcccgcttga
     1561 gaaactgcca accgctttat aacaaattcg tctttgcacc aaactttcca ttctttccgt
     1621 ttttcggacg gcatcgttaa agtagtcctt ccttttcctt attttcagca ttgttttatg
     1681 ttactgctca aaacgcccgg cgtactgccc ggcttcaaac tcagccttgg tctgaccgta
     1741 ttgtgcctgt cgctgctggt ggttttgccg tttgcgatga tggcggcgaa ggcggctgaa
     1801 atcggctggg gcggcttttg gaacacgatt gccgagccga acgtattggc ggccgtctgg
     1861 ctgagtttgc ggatgtcgtt ttatgcgatg ctgaccaatg tcgtgttcgg cacgctggtg
     1921 gcgtgggtat tggtgcgtta tgaattcccg ggcaagggtc tggcgaacgc gctggtcgat
     1981 ttgccgtttg cgctgccgac ggcagttacg ggtatcgcgt tggcaaccct gtatgcgccc
     2041 aacggttgga taggccgttt tttcgagcct ttgggcatca aaatcgcgtt tacgcccgtc
     2101 ggcatttgga ttgcgctggt cgtcgtcagc ctgcccttta tcgtccgcgc cgtgcagccg
     2161 gtgttggagg aattatcggg cgaatatgag gaagcggcgg cgactttggg cgcaaaccgc
     2221 tttacgacgt tccgccgcgt gctgctgccg gaaatcacgc cggcactctt gaccggcgcg
     2281 ggaatgatgt tcgcgcgggc aacgggggaa tacggctcgg tgatttttat cgcgggcaac
     2341 attccgatgg tttctgaaat cctgccgctg attattacgg gcaagctgga acagttcgac
     2401 gtgcagggcg cgtcggcggt ggcgttgttt atgctgctgg tttcgtttgt gattctgttt
     2461 gcgctgaacg tgatgcagtg ggcgttgggc aggcgttcgg gcgcgaaggg ttgaggtcgt
     2521 ctgaaatacc tgttaccgtc attcccgcgc aggcgggaat ccattggtga atttcggctg
     2581 ccttatttat tttctgtttt cctgttgccc tgcggtggat tcccgcctgc gcgggaatga
     2641 cggtagctag acgtttttat tcccttaatc aataaaaggt tgtctgaaaa cgaatccgcc
     2701 ccacaaaaaa cggtttttca gacggcatcc gaacacctta aaacaaccaa ccggggaaca
     2761 ccaccgccat gaaaccctat tccgccaatc ccaacctgac cgaaccgcgc cggctgcgcg
     2821 tgttgctgat tgccgccgcg ctgggctttc tgctgctgat actggtcgtg ccgctcgtcg
     2881 ccgtgtttta cgaagcctta aaaggcggtt gggatttgta cctgaaatcc ttaaacgatc
     2941 ccgaagcgtg gtctgccatc aaattgacgc tgattaccgc gctgattgtc gtccccgtca
     3001 atgccgtctt gggcgtggcg atggcgtggc tgctgacccg ttttgacttc cgaggcaagc
     3061 agttgctgac taccctgctc gatttgccgt tttccgtatc gcccgtggtg gccggtttaa
     3121 tgttcgtctt attgttcggc gcgcacacgg cattgggtgg ctggctcgaa gcgcaaggca
     3181 tacagattat cttcgccatc cccggcatta ttctggcgac gctgttcgtt accttcccct
     3241 ttgtcgcgcg cgaaatcatc ccgctgatgc aggcgcaggg cgacagcgaa gaacaggccg
     3301 cgctgatact cggcgcaagc ggctggcaga tgttttggcg cgttaccctg cccaacatca
     3361 aatgggcgtt actctacggc atcatcctca ccaacgcccg cgcgatgggc gaattcggcg
     3421 cggtcagcgt ggtatcggga cacatacgcg gcgaaaccaa caccgtcccg cttttggtcg
     3481 aaatcttcta caacgaatac aactttaccg gcgcattcgc cctctccggc gtattggcac
     3541 ttttagcact ggcgacgctg gcggtgcaaa acatcattac caaattacaa gacaaaaaac
     3601 tcgccgccgc cgaaaggaac acagtatgag tatcaccatc caaaacttaa acaaacactt
     3661 cggcaatttt cacgcgctga aaaacatcaa cctcaacgtc cccaccggca aactcgtttc
     3721 cctactcggc ccgtccggct gcggcaaaac cacgctttta cgcattatcg ccggactgga
     3781 aaacgccgac ggcggccaaa tcctctttga cgggcaagac gtaaccgcca aacacgtgcg
     3841 cgagcgcaaa gtcggcttcg tgttccaaca ctacgccctc ttccgccaca tgaacgtgtt
     3901 tgacaacgtc gctttcggtt tgaccgtatt gcccaagtcc gaacgcccgt ccaaaggaca
     3961 aatccgcgcc aaagtcgaag aattactcaa gctcgtgcag ctctctcatt tggcaaaatc
     4021 ctatccgcac caactctccg gcgggcaacg ccagcgcatc gccctcgccc gcgcgcttgc
     4081 ggtcgaaccc aaactcttgc ttttggacga acccttcggc gcgttggatg ccaaagtacg
     4141 caaagaatta cgcacctggc tgcgcgacat ccatcacaac ctgggtgtaa ccagcattct
     4201 ggttacgcac gaccaagaag aagccctcga agtttccgac gaaatcgtcg tgatgaacca
     4261 cggcaaaatc gaacaaaccg gcagcgccga agctatttac cgcaaacccg aaaatgcctt
     4321 cgttaccgag ttcctcggcg aaaccgacgc ttttgaagga cgcatcgaaa aaggcttctg
     4381 gcattatagc ggcttcgcgt ggaaattgga cgcacaatac aaatggcagg aacaaaccgc
     4441 caccggctat atccgcccgc acgaatggca gatcgccgcc gaacacgaaa caccgatgat
     4501 ttgtgccgaa atcgaaaaag tccacgccgt cggggcattg acgcatattt tggtgaaaca
     4561 cggcaaacaa gacgtgcaca tcacgctggc tggcagcgat gccgcgcgtt acccaatcgc
     4621 cgaaggcaaa gcattgaagc tgattccgaa acaggtttat gtcttctctc aaaacgaact
     4681 gattgaatat tcgatttaac catgaaagcg caatgccgtc tgaaaggctt tcagacggca
     4741 ttgtgctttc aagcgtcagg caagaaacag cttgtacgcg gcattttgcg tttcctcgtg
     4801 atagctgtat cccagacttt ccaagaaacc gtcaaatgcg gcggcatcgt gcggcggcac
     4861 atcgataccg accagaatcc gcccgtaatc cgcaccgtgg ttgcggtaat ggaagagcgt
     4921 aatgttccaa ccgccctgca tatggttcaa aaaacgcgcc aacgcccccg gacgctccgg
     4981 aaactcaaaa ctgaccagac gctcgttttc tactttgtcc gtccgccctc cgaccatata
     5041 gcggatatgg attttggcaa tctcatcatc ggtcaggtcg acattgggca atcccgcctc
     5101 atccaaccgg ctaccgataa ccgccaaatc ctgcggacct gccgcttgaa gtccgacaaa
     5161 gatatgcgct ttttcatcgt ccccgtagcg gtagttgaac tcggtaatat tcctatttcc
     5221 caatatattg acaaacttaa ggaagctgcc gcgctcttcg gggatggtaa cggcaaaaat
     5281 accttcgttg ccctcgccca attcgctccg ttccgaaacg tggcgcaaac ggtgaaaatt
     5341 catattcgcg ccgctggtaa cggcaatcag ggtttggttt tccgcgcctt ctcgggcgat
     5401 ataggctttc agacctgcca atgccaatgc acccgccggc tcggtaatgc tgcgcgtgtc
     5461 atcgaaaata tccttgaccg cgccgcaaac cgcatcggta tcgactgtaa tgatttcatc
     5521 caaaagttct ttgcagaggc ggaaggtttc gtcgccgaca actttgaccg cagtgccgtc
     5581 tgaaaacagc ccgacatctt tcaaatggac gatttcgccc gcttcaaccg attgcttcat
     5641 acagcaggaa tcgttggtct gaacgccgat aactttgatt tcggggcgga cctgcttgat
     5701 aaatgccgcc acgcccgccg ccaaaccgcc accgcctatc ggtacgaata cggcgcggat
     5761 tggatcggga tgctggctga caatttccat ccccaccgtc ccctgtcccg caatcacatc
     5821 aggatcatca aacggcgcga tataggttaa cccttctttt tccgccaact ccatcgcata
     5881 atcgtaggca tcgttgtatg aaacgccccg caaaaccacc tcgccgccat ggcttttaac
     5941 cgcatccact ttgattttcg gcgtagtttc cggcataacg ataacggcac ggcaacccaa
     6001 acgctgtgcg gacaatgcca cgccttgtgc gtgattgccc gcgcttgccg caatcacgcc
     6061 gcaagcgagc gcatctttcg gcaacttgga cattttgttg tacgcgccgc gtattttgaa
     6121 cgaaaaaacc ggctgcaaat cttcgcgttt caaaaggatg ttgtttttca aacgtacaga
     6181 aaggctgcgt gccggttcca aaggcgtttc gaccgcgaca tcatagacag atgccgtcag
     6241 gatgcggatg aggtaatcgg aataaggaag gggcgtgttc ataattcaat atgggataat
     6301 cggtttatta aaatcgcaaa acccaaaacc atacgcccaa gacggcgcga aagcaagaaa
     6361 aatccgcccg atcagacacc ctaagcgtat aatcggcagg ctgaaacacg cacacaatta
     6421 gaatatttca tgacagcaca taaaatcctg cccgtcctac tttccatcat cttaggcgtt
     6481 tctcacgcaa cggccgcatc gcccgcgccc aacagaccga cggtacacgc cgcccccacg
     6541 ctccaaacac ccgaaaccct cacagcggca cacatcgtta tcgaccttca aagcagacag
     6601 actttatccg ccaaaaacat caatacccct gtcgaaccgg cggcactaac ccaactgatg
     6661 accgcatatt tagttttcaa aaacatgaaa tcgggaaata tccgatctga agaaaactta
     6721 aaaatacccg aatccgcatg ggcttcagaa ggaagcagaa tgtttgtacg tcccggcgat
     6781 acggtcggca ccgacaaact cttaaaaggc atgattgccc tatccgcaaa cgatgccgcc
     6841 ctaacccttg ccggccgact gggcaacggc tcgattgaaa attttgtgca acaaatgaac
     6901 aaagaagccc gacgcttggg catgaagaac actgtattca aaaacccgac aggcttgagt
     6961 agagaaggac aggtttccac cgccaaagac ctcgccctgc tgtctgaagc attgatgcgc
     7021 gactttccgg aatattaccc gctgttttcc atcaaatctt tcaaattcaa aaatatagaa
     7081 caaaacaacc gcaatatcct tttatatagg gacaacaatg taaacggtct gaaagccgga
     7141 cacacagaaa gcggcggcta caaccttgcc gtgtcataca ccggaaacgg caggcatatc
     7201 cttgtcatca cattgggttc ggaatcggcg gaaacacgcg catcagacaa cagcaagctg
     7261 ctgaactggg cattgcaggc cttcgatacg cccaaaatat atccgaaagg caaaaccgtt
     7321 gcccaaatcc aaatttccgg aggcagcaaa aaaaccgtcc gcgcaggctt cctcaaagaa
     7381 gcctacatca ctctgccaca taaggaagcg aaaacagccg aacaaatttt ggaaaccata
     7441 cagccgattc ccgccccggt aaaaaaaggg caaattttag gaaaaatcaa aatcagacaa
     7501 aacggataca ccattgccga aaaagaaatc gtcgcactgg aaaatgtaaa aaaaagaagc
     7561 cggtggcaaa ggctttggat gcgtctgacg ggacagtaat ctgccgtttc aaatatcccg
     7621 tttttccaac aaataaagaa atgccgtctg aaacacggtt cagacggcat aaaacaacag
     7681 ggcggtacgt attgcatacg caccgccctg ctgctgaaat caattagcgt tttttaccgg
     7741 taacggtggc aacagccaga ttttcgttac gtttcaagga aacgctttct acaccttcag
     7801 gcagtttgat gtcggacaaa tgcaaaatgt cgccggcaac cacttcggca caatccaaat
     7861 ccaagaaagc agggatattg gcaggcaacg caagcacttc aaccgtcgtg ttcaacagag
     7921 atacacggcc gccttgcagt ttgaccgctt gggaattttc agcgttaacg atgtgcaggg
     7981 gaacacggat acgtacaagt tgatcagctt tcacagcttg gaagtcgata tgttgaactt
     8041 cgcggcggaa cgggtgcatt tggaaatcgc ggacgataac gtctttagtt tcaccgttca
     8101 gagacaactt aatcaacgca gtatggaaag attctttttc caatgcgtag aatacggttt
     8161 tgtgatccac agcgattgca acaggctctt gaccttcacc gtacagaatg ccggggattt
     8221 ggccttcgcg acgcaggcgg cggctcgcac cagtgccttg tgcttcacga acagaggctt
     8281 gaatttcata agtcatgtta aatactccaa gttaggtaaa atcgccgtca tcggccgcga
     8341 ccagcttaag acggcttcgg gcttatggca gcaacatact gcctgtcatc acttcttcat
     8401 taaagagata tgaaacggat tcttcattgc tgatgcggcg gacggtttcg gccaacagac
     8461 cggcaatcgt tacctgacgg atacggtcgc agtttttagc cgcttcagac aaaggaatgg
     8521 tatcggttac gaccacctgg tcgatttcgg atgaggcgat acggctgacc gcctctccgg
     8581 agaatacggc gtggctggca tatgccaaga cacgttccgc cccccgctct ttcagagcga
     8641 cggcggcttt gcacagcgta tttgcagtgt caatcatatc gtccacaatc agacaggttc
     8701 tgccttgaat atcgccgatg atgttcatga cttctgccac attggctttc gggcggcgtt
     8761 tatcgatgat tgccaagtcg gcattcaagg attttgccac ggcgcgggcg cggacgacac
     8821 cgccgatgtc cgggctgacg acggtcagat tttcaatccg ctgttgtttg atgtcgttca
     8881 acagaatcgg ggtggcataa atattgtcca ccggaatatc gaagaaacct tgaatctggt
     8941 cggcatgcaa atcgacagtc aaaacacggt cgatccctgc cgaatacagc atatttgcca
     9001 ccagtttggc agaaatcgga acgcggacgg aacgcggacg gcggtcttgg cgcgcatagc
     9061 cgaaatacgg aatggctgtg gtaatacgac ctgccgaagc acgcttcagt gcatccgcca
     9121 tcgtcaggat ttccatcagg ttgtcattgg tcggcgcaca ggtcggctga aggatgaaaa
     9181 catcgcgccc gcgtacgttt tccaacagtt cgacggcaac ttcgccgtct gaaaacttgg
     9241 atacggaagc attgcccaaa gaaatgtcca aatgcctgac aacacgttgt gccaattcgg
     9301 gattggcatt gcctgtaaat accatcaaac tgtcgtacgc agccatattc tcacctgatt
     9361 ttatgtttaa cttccgctca gaaaacacaa tgcttccctc tgcttgtacc aagcccgaca
     9421 ttatacctgc aacatccgac acaaacaaag gcatttcaat atttttattt ctatgaaata
     9481 aaagcgtgta agcaggctta cacgctttta tctggctggg gaggaaggat tcgaaccttc
     9541 gcatgctgga atcaaaatcc agtgtcttaa ccgcttgacg actccccaaa aagggctggc
     9601 tggggaggaa ggattcgaac cttcgcatgc tggaatcaaa atccagtgtc ttaaccgctt
     9661 gacgactccc caactcgttt gacttggctg gggaggaagg attcgaacct tcgcatgctg
     9721 gaatcaaaat ccagtgtctt aaccgcttga cgactcccca acaatgttta tacggacaac
     9781 aaaggatgct ttgaaagacc ctctgccaaa tatgcctcgt acaaacctga aacttgtcgg
     9841 tatatattgt atgcgctatt cctatcttga cacgccgtga atacacatgc cccggaacct
     9901 gtcattaagg caaatccata tcgggacaac tcggaatagg ctttccaaac ttcagggtat
     9961 tctttaaata caactgcctg catatcattt ctaaacggtc gcagattttg gaaagtcggc
    10021 attatgcttg aggcagaatc ccgtgtcaag ctttcgtgtg tgaaaatttt tgcagtggaa
    10081 acgtggacgg gcggtttgac gatgacatac cactgtttcg gaatatccat ttcgtccagc
    10141 ctgtcgccta taccccgcgc aaacgcattt tttccgaaaa taaaaaacgg tacgtccgcc
    10201 cccagagccg cgcccgaatc aatgagctgc cgctgcgtca gaccgcactg ccaccaacgg
    10261 ttcaacacca gcaaaacggt tgccgcatcc gagcttccgc cgcccaaacc cgcccctgtc
    10321 gggatttttt tgtccagcca tatttccacg ccggcggggt tgcgcgcata tttttgcagc
    10381 aacgatgcgg cacggtagct caaatcggct tcctgcggca tgccatcaac cggattgtgc
    10441 aggatgattt tgccgtcgtc ccttagtttc aaatatacgg tatcctgcaa atctatcagg
    10501 cagaatatgc tttcgatatt gtgataaccg tcttcccgct ttccagtaat cctcaaatcg
    10561 agattcagtt ttgcaggtgc gggaaacgcc tgccgtccgt ccgcaatatt catctgtccg
    10621 ccttatctcg tgcgcgccgc acagcgttcc ggggtttcag tttcagacgg cataccgatt
    10681 tcggtgaaaa ccagcctgat gttcaaattt ccgttattca gttgcaacgt tcggacttgc
    10741 cccccactgt cggcggttct gccgacagtc caaccgtatt gttccaatat gccgtccggc
    10801 aggatgcggt aaggcgcgcc cgccacacgc ctgccatctg cccagatatg cagatattgg
    10861 attggcagtt tgaaaccgac cagctgcctg ctcaattctt ccgcactttc cgcctgatag
    10921 acatttcctt tgccgtccac tgccaatgcg ccgtccctgt cttgacacaa ctgcccgagc
    10981 gtactgccca aaggggtatt gatattgatg gtttccacgg gcggttggta tgtccaatcg
    11041 aaatttgcat acgaaccttt cccttccgct ttcactgcca accgcccttc cgccgtgaaa
    11101 ctgcgggtgt gttcggacgg ctgccacagg ttttcgttat tttgaggcaa ttgcgcgcaa
    11161 gcggtcaaaa gcaggatgac cgatgcggat acggtgtgtt tcatcagaat ttccttaacg
    11221 ggtgccatcc tgccggccgg cgggttcggc gtgccgtgca aaacgcgtgc gaactgcgtg
    11281 ccgtttgccc gaatgccccg gcttcccggc aaaccttatg ccgtctgaaa ggatggacct
    11341 gcattatttc cgaggttttc gggaaggttg gggcaatgcg atgccgtgac gtttgagcgt
    11401 ttcccgccat attttcttgt ctcccgtaag gtgtgccgcc tgcgtccata cgtcaaccgc
    11461 ctgatcgcgt tcgcccaatg cccacaacac ttcgcccaaa tgggcggcaa cttcgggctc
    11521 ggggtcgttt tcaaacgaat accgcagata cggcagcgcg ctttccgcgt cgcctttcag
    11581 gtaatacgcc cagcctatgc tgtcgttgac agcggtatcg tccgggttga tttggtatgc
    11641 cgtctgaagc agggcgaagc cttcgtccaa acgtttggaa tcggaaagca ggctgtagcc
    11701 cagattattc ataatctgag cgttatcggg tgcaagcctg aacgcccttt caagatctga
    11761 aatcattttt ttccgcttgc caagccgatc gtaaacaact gaccgctgta ccaatgcctc
    11821 tgcctgtaac tctgtattac tgccggcagg cggtttttcg ataatcttgt ccaaccccct
    11881 caaagcctcc cgtttatcgg gcagcttcga cagggcgagc atctgtattt tggacaaatt
    11941 gtctgccgta aaataccgcc cctgctgttc gggaagtttc cgcaccctgc cgatctgccg
    12001 caaagccgcc ctgccgccgt ccaactcgac agccgccgca gccgccagca cacctttgtc
    12061 gaacaggtat tccggcgcgg acactttttt caaccactgc ctgactttgg tgtaatccct
    12121 tcggtcggca tatatcatcg ccgccgtcat tgccgccctg ctccgctgtt cctccgtccc
    12181 cctgccgtat gccttttcgg cgtagccgtc gataacggaa gcaccttctt ttcggtttgc
    12241 cgccaatatc gctgcctgaa tatacaggtc tgcattcgga ttgcgttcca acagcacgtt
    12301 caaacgcgca taggcatcat ccagcctgtg cagggaaacc agattcataa tttccatttc
    12361 ctgccagacg gccgaaaggt tttgggtgtc tgtctgctcg aaaaagccgt cgagtatttc
    12421 gggatatttg cgtgcagtca gacgcaacgt cattaaagtg gggggcaata tttccgtatc
    12481 gagcttcgcc aaacgctgca aagctccgat tgccttttcc ttttcgcgtc cctgtacgct
    12541 gaacaccaca tcggcaaccg ccgcttcggg cagatgttca tatttcaacg ccgcgcggcg
    12601 aaccgctttc gatgcttttt gcgccaatcc gtcctgctgc acggcggctt gtgccagcag
    12661 caaaaatatc ctgcgttttt gcacatcgtc cgattgtgcc agcacctctt ccagtccgtc
    12721 cagatgctga tttccccttt ccctcagcac gttccgcagc caccccgccc gtttttgcgc
    12781 cttacccggt ataggctcaa tctgccgcca tttctgataa atcatttccg cctgttcaaa
    12841 cgcgttcagc gacacggcca tttccaaggc gcgttcggcg acttcggggg attttgtacg
    12901 ctccaacatc agcatatagg ttgccagagc cgttcccgcc tgcccctttt gcaaggcggt
    12961 ttcccctccc agtaacgtaa atatctgatt aacccgctcg cccactgccg caagccgtgc
    13021 gcgttcgttt ttgatttctt cctcactgta acgctgctgc tttctgaaaa cctttacgac
    13081 ttccttcggc tgtttcatat cccccgcacc gcctccggcg gcagatacct gtccggcaat
    13141 caaggttgcc gtcaacacag ttaacatttt gaaacggtta ggtaacataa tcatccttta
    13201 tccgacagcc gtccgaacct tcagacggca tataatcagg cgtaacttta tcacaagcag
    13261 aatcgtttgt agatttgcga aaccccgttc aaaaaaataa atg
//
LOCUS       DAXHTY010000055        12889 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXHTY010000055 DAXHTY010000000
VERSION     DAXHTY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.055"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(344..1786)
                     /gene="aldA"
                     /locus_tag="ACFNMK_001309"
     CDS             complement(344..1786)
                     /gene="aldA"
                     /locus_tag="ACFNMK_001309"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFC6364576.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEEAIAREPKGGRADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1821..2021)
                     /locus_tag="ACFNMK_001310"
     CDS             complement(1821..2021)
                     /locus_tag="ACFNMK_001310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364577.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTGANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTVIYLGIN"
     gene            complement(2030..2419)
                     /locus_tag="ACFNMK_001311"
     CDS             complement(2030..2419)
                     /locus_tag="ACFNMK_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364578.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIEFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTVDRISVSFIREIQAA"
     gene            complement(2420..2596)
                     /locus_tag="ACFNMK_001312"
     CDS             complement(2420..2596)
                     /locus_tag="ACFNMK_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364579.1"
                     /translation="MSGYVQFLGTDSKGQSKFIFVGTNENGSITTIHTKSGKDFWRTL
                     NNNPKNKTIYPKAR"
     gene            complement(2627..3544)
                     /locus_tag="ACFNMK_001313"
     CDS             complement(2627..3544)
                     /locus_tag="ACFNMK_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6364580.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLNFGINRSRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYACFTPILYREPVRIWNLSPNLRGLAASCLQAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLADALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3717..4517
                     /locus_tag="ACFNMK_001314"
     CDS             3717..4517
                     /locus_tag="ACFNMK_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6364581.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSVMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4565..5341
                     /gene="mlaE"
                     /locus_tag="ACFNMK_001315"
     CDS             4565..5341
                     /gene="mlaE"
                     /locus_tag="ACFNMK_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC6364582.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5392..5886
                     /gene="mlaD"
                     /locus_tag="ACFNMK_001316"
     CDS             5392..5886
                     /gene="mlaD"
                     /locus_tag="ACFNMK_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC6364583.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGNSGKTYTVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5923..6513
                     /locus_tag="ACFNMK_001317"
     CDS             5923..6513
                     /locus_tag="ACFNMK_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC6364584.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGGK"
     gene            6564..6842
                     /locus_tag="ACFNMK_001318"
     CDS             6564..6842
                     /locus_tag="ACFNMK_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC6364585.1"
                     /translation="MHTELKNGILHIGGDITVKTLTAAAFTRFRQQCRLKDTCAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6839..7666
                     /locus_tag="ACFNMK_001319"
     CDS             6839..7666
                     /locus_tag="ACFNMK_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC6364586.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7791..8153
                     /locus_tag="ACFNMK_001320"
     CDS             7791..8153
                     /locus_tag="ACFNMK_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC6364587.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIETRFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            8153..8536
                     /locus_tag="ACFNMK_001321"
     CDS             8153..8536
                     /locus_tag="ACFNMK_001321"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6364588.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            8533..9021
                     /locus_tag="ACFNMK_001322"
     CDS             8533..9021
                     /locus_tag="ACFNMK_001322"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC6364589.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(9104..9232)
                     /locus_tag="ACFNMK_001323"
     CDS             complement(9104..9232)
                     /locus_tag="ACFNMK_001323"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC6364590.1"
                     /translation="MAGVTVGRGSVVGAGAVVTKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(9238..9618)
                     /locus_tag="ACFNMK_001324"
     CDS             complement(9238..9618)
                     /locus_tag="ACFNMK_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC6364591.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
     gene            9628..9754
                     /locus_tag="ACFNMK_001325"
                     /pseudo
     CDS             9628..9754
                     /locus_tag="ACFNMK_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9856..10071
                     /gene="rpmE"
                     /locus_tag="ACFNMK_001326"
     CDS             9856..10071
                     /gene="rpmE"
                     /locus_tag="ACFNMK_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC6364592.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            10386..11000
                     /locus_tag="ACFNMK_001327"
     CDS             10386..11000
                     /locus_tag="ACFNMK_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC6364593.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILVENNSFDMLWTVLG"
     gene            11161..12822
                     /locus_tag="ACFNMK_001328"
     CDS             11161..12822
                     /locus_tag="ACFNMK_001328"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC6364594.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLACRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAANPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENI"
BASE COUNT         2943 a         3484 c         3245 g         3217 t
ORIGIN      
        1 aagtggcggg aatgacggtt cgggcattcc ttaaatcacc cgtgtatcgc tgtaaatctt
       61 agagatggcg gaatatagcg gattaacaaa aaccagtacg gcgttgcctc gccttagctc
      121 aaagagaacg attctctaag gtgctgaagc accgagtgaa tcggttccgt actatctgta
      181 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataca aagacaaaaa
      241 catcgatgtc ggtcatgact gattgccggt gaaactgtaa aaatgccgtc tgaacctgaa
      301 aacgggttca gacggcattt tctatcgggg ttttaaagcg gcattaaatg tcggtttcca
      361 gataaacgac ttgggtttgc agatattctt ccaaaccgtg tttgccgtcc gcgccgccga
      421 tgccggattt tttccagccc gcgtggaagc cttgcatggc ttcaaagttt tcgcggttga
      481 tgtaggtttc gccgaattgc aggcggcggg taacgtagaa ggcttcgttt aaattagtcg
      541 tataaacaga actggtcaga ccaaactcgc aatcgttagc cagtgcgatg acttggtcaa
      601 gcgtgtcgaa agcggaaacg ggcagaacgg ggccgaaggt ttcttctttc ataatgtcca
      661 tactgttgtc ggtgtcggtc agcagggtcg gctcgaagaa ataaccgcgt ccttcggcgc
      721 gtttgccgcc gcagaccagt ttcgcacctt gtttgactgc ccgttccact ttttcggcaa
      781 cggctttgac ggcgcgctct tcaatcagcg ggcccatttc cagcgcgcct gcttcggctt
      841 cggcagggtt gccgtagcgc acgcctttca tggcggcagt cattttttca atgaatgcgt
      901 ctttcagact gctgtggaca taaacgcgct cggcgcagtt gcagatttga ccggtgttgc
      961 cgacgcgcga agccaagatg gatttcaccg ccaaatccaa atccgcgtct ttcaaaacga
     1021 tggcgggggc tttgccgccg agttccagcg aaactttggt gatgttggcg gaagcggctt
     1081 ccatcacttg gcggcctgct tcgacggagc cggtcaggct gaccatatcg acttgcggat
     1141 gggcagacaa ggcattgccg atttccgcac ccggaccgtt caccacgttg aacacgcctg
     1201 cgggcaatcc gaccgcatcg acgatttcgg cgaagatgtg gcagttgatc ggggttacgc
     1261 tgctgggttt gacaacgatg gtgttgcccg ttactaaggc gggacccatt ttgcgggcaa
     1321 tcaggaagaa agggaagttc cacggcaaaa tgccggcaat tacgcccagc ggacgtttga
     1381 acaataaaat gttttcgcgc gggcggtcgc tttggatgat ttcgccttcg taacggcgcg
     1441 cccattcggc ttggtaatcg agatagtcgg cggtgaacat cacttccacg cgcgccaagt
     1501 ctttggtttt gccgccttcg gcaacgatgg tgtcggtcag ctcgtcggcg cgttcgcgta
     1561 tgccttgggc gattttgcgc aaatacgcgc cgcgttcgac cgcaggcaga cgctcccaag
     1621 ccggttgcgc cgcacgcgcc gccgctacgg cgcggtcaac gtccgccctg ccgcctttgg
     1681 gttcgcgggc gatggcctct tcggtggacg ggttcaatac gtcgcgccat tcgccgttga
     1741 aatcgttttc aaagcgtccg ttgatgtaca tggccaattg tttcatttcg ggttctccag
     1801 ttttgtagtc agatgtagtt ttagtttatt cccaaataaa ttaccgtaca agtttctttt
     1861 gaaatgttta gacgagaagc tactatgtct gcatattgtt ctgctaattt atattcactc
     1921 gaatgcaact gccgattatt ccaatctgca ttcgcaccag taaaagcccc agcattgccg
     1981 cctgtggcat aaccaatcgc taaactgcca acggtatcca caacaggttt caggctgcct
     2041 gaatttctct aataaatgaa acacttattc tatcaaccgt taatgtaacc aaacaattat
     2101 caagataaga gtagtttatt aaatactcat cttcttcaaa ttgtatattt tgtagggtta
     2161 gatgattatt ttttaattct gccgttattt catacttgaa actatcatct atttttctaa
     2221 attcaatatt tgaagttgat ttatccagta tgtaatcatt aaagatttca taggataatt
     2281 ccacttcata aaattgattt tcaaatattt tgaatgggca gtacgatata aaacaatgta
     2341 gttcataacc atttatttca atcaatactg cctcttcaat ggtgttatct aactttttga
     2401 ttttacattt atataacatt tatcttgcct ttggataaat tgttttgttt tttggattgt
     2461 tattcaaagt tctccaaaag tcttttcctg attttgtatg tatcgtggta atactaccat
     2521 tttcattggt tccaacaaaa ataaatttag attgaccttt ggaatcagta cccaaaaact
     2581 gaacatatcc agacatgcgg atttcgggtt ttaaatcaaa tgcggcttac cggttttctt
     2641 ttttggtttc cgcaggcgtg ctgccgaaat attgcctgaa tgcctgagta aagctggaaa
     2701 cgtggcggta gccgcacaaa tacgcggttt cgcctatgct tttgcccccg ttttgcaaca
     2761 ggtagagcgc gtgctgcatt tgtttgtggt gcagccactc gcttgcggta atgccgaaat
     2821 ggtcgcgcat acggcgttgc agcgtccttt cgctgatgtt cagcgcgtct gccagccggt
     2881 tgacttggtg tgcgccgtcg gcaaacgcgg catttagggt gcggctgaag tcttcagacg
     2941 gcatagcgtc tgcttccgcc gtttgccccg ccgccggccc gatgccgtct gaaaccgtgt
     3001 cccacaaatc cgacagcagc cgcaaaacgt ccgcctcgcg gcgcaatgtt tcgcccaaat
     3061 gcccctttgg gacggcttgc aggcaggatg ccgccaaacc gcgcaggttt ggggacaaat
     3121 tccatattct gaccggttcg cggtaaagta tgggtgtaaa acacgcatac tccggacgca
     3181 gcagccattg ctccatacct ttaattgtca ttttgaccgt tttgccgcct cggtaaagat
     3241 agcggctgaa caagacttct tccccgacag caatcaggac gatcttgcca ccgtccgcat
     3301 cgatttggaa tcggctccgg ttgatgccga agttcaaaga gccttccagc aacagcacga
     3361 acgacacata aggttcggcg aggcggctgc tgcaaaaatc acaccgtgcc gttactgtgc
     3421 cgccgtggat ggaaatgccg ttggacaaag tatcgaaacg gtagctgccc tccacatagc
     3481 tgccgccgcc gcttttgact agattgacga accggctgct gttcaaatgt tctgcttttt
     3541 ccatagtttt ctgataaagg ttatcatttg aaagataaca tttttcgcca cagcaaacaa
     3601 tccgccgccg acaacggcat tttcacaaca tttaccgtcc gtatgccgtc tgaaaattgc
     3661 ccgtcttccc gataagcctt aaaatacacg ccgtcaaacc taccgtccgt ccgtacatga
     3721 gcccatcccc ctttatcgaa atgaaagacg tcgccttcgc gtatggcgac cgcccgattc
     3781 tgaagaacat caatttcagc attccgcaag gcaattttgc tgccgtaatg ggcggttcgg
     3841 gcagcggcaa aaccacgctg atgcgactga ttacaggaca gattcgtccg cagtccgggc
     3901 aggttttgat tgaaggacgg gatttggcgg gcttttcggc tgacgagttg tatgaacacc
     3961 gccgccgtat gggcgtatta ttccaacacg gcgcgctgtt taccgatttg tcggtattcg
     4021 acaatatcgc ttttccgatg cgcgaactga cacgcctgcc ggaagcggtg attcgagatt
     4081 tggttttgtt gaaactgaac gcggtcggtt tgcgcggcgt ggaaaacctg atgccgtccg
     4141 agttgtccgg cgggatgtcg cgccgcgtcg cgcttgcccg cacgattgcg ctcgaccctg
     4201 aaattatgtt gtacgacgaa ccgtttaccg gcctcgatcc gatttccttg ggcgtgattg
     4261 cccacttgat cagccgcgtc aataaggctt tgcgttcgac cagcgttatg gtaacgcacg
     4321 acattgaaaa atctttggaa atcgtcgatc aggtgatttt tttggcgcac ggcgaaatta
     4381 tgttctccgg ctcgccgcag gaaatgcgcg aactggattc gccttgggtg cgccagtttg
     4441 tcggcgggct ggcagacggc cccgtagcat accgctatcc ggcgcaaacg tcgttgcagc
     4501 aggatttgct cgggtaaatg agcaggtgcc gtctgaaacc gttgaacttt acatatagaa
     4561 atttatgaat tttatccgtt ccgtcggggc gaaaaccctc ggccttattc aatccctcgg
     4621 cagtattacg ctgtttctgc tgaacattct ggcgaagtcc ggtacggctt tcgtccgtcc
     4681 gcgcctgagc gtgcgccaag tgtatttcgc cggcgtgctg tcggtgctga ttgttgccgt
     4741 ttcggggctg ttcgtcggta tggttttggg tttgcagggt tatacgcagt tgtcgaaatt
     4801 caaatccgcc gatattttgg gctatatggt cgcggcttcg ctgttgcggg aactgggtcc
     4861 ggtgttggcg gcgatcctgt ttgccagcag cgcgggcggt gcgatgacca gcgaaatcgg
     4921 tctgatgaaa acgaccgaac agctcgaagc gatgaacgtg atggcggtaa accctgtcgc
     4981 ccgcgtggtt gcgccgcgct tttgggcggg cgtgttttcc atgccgcttt tggcttcgat
     5041 tttcaacgtg gcgggtattt tcggcgcgta tttggtcggt gtaacctggc tgggcttgga
     5101 cagcggcatt ttctggtcgc aaatgcagaa caacatcacg atacattacg atgtaatcaa
     5161 cggtctgatc aaatccgccg cgttcggcgt ggcggtaacg ctgattgccg tgcatcaggg
     5221 cttccactgc gtcccgacct cggaaggcat tttgcgcgcc agcacgcgca cggtggtttc
     5281 gtccgccctg acgattttgg cggtcgattt tatattgacc gcgtggatgt ttacagattg
     5341 acagggcgtc tgaagacgaa acgcggaaca tcggatattc aaggaacttt aatgaaaaag
     5401 aacatattgg aattttgggt cggactgttc gtcctgattg gcgcggcggc ggttgccttt
     5461 ctcgctttcc gcgtggcggg cggcgcggcg ttcggcaatt ccggcaagac ttataccgta
     5521 tatgccgatt tcggcgacat cggcggtttg aaggtcaatg cccccgtcaa atccgcaggc
     5581 gtattggtcg ggcgcgtcgg cgctatcgga cttgacccga aatcctatca ggcgagggtg
     5641 cgcctcgatt tggacggcaa gtatcagttc agcagcgacg tttccgcgca aatcctgact
     5701 tcgggacttt tgggcgagca gtacatcggg ctgcagcagg gcggcgacac ggaaaacctt
     5761 gctgccggcg acaccatctc cgtaaccagt tctgcaatgg ttctggaaaa ccttatcggc
     5821 aaattcatga cgagttttgc cgagaaaaat gccgacggcg gcaatgcgga aaaagccgcc
     5881 gaataaacgt atgccatcat attcaaacta ggacacttaa tcatgaaaaa atcctccctc
     5941 atcagcgcat tgggcatcgg tattttgagc atcggcatgg catttgccgc ccctgccgac
     6001 gcggtaaacc aaatccgtca aaacgccact caagtattga gcatcttaaa aagcggtgat
     6061 gccaacaccg cccgccaaaa agccgaagcc tatgcgattc cctatttcga tttccaacgt
     6121 atgaccgcat tggcggtcgg caacccttgg cgcaccgcgt ccgacgcgca aaaacaagcg
     6181 ttggccaaag aatttcaaac cctgctgatc cgcacctatt ccggcacgat gctgaaatta
     6241 aaaaacgcca acgtcaacgt caaagacaat cccatcgtca ataaaggcgg caaagaaatc
     6301 atcgtccgcg ccgaagtcgg cgtacccggg caaaaacccg tcaacatgga cttcaccacc
     6361 taccaaagcg gcggtaaata ccgtacctac aacgtcgcca tcgaaggcgc gagcctggtt
     6421 accgtgtacc gcaaccaatt cggcgaaatt atcaaagcga aaggcgtgga cggactgatt
     6481 gccgagttga aagccaaaaa cggcggcaaa taaccgacaa accataccgt ccgagggcgg
     6541 tatgggtttt ccgagcaaac gctatgcata cagaactcaa aaacggaata ttgcacatcg
     6601 gcggcgacat caccgtcaaa accctgaccg ccgccgcttt tacccgcttc agacagcaat
     6661 gtcgtctgaa agacacctgc gcggttgatt tgagcggcgt cggacgcgcc gattccgcct
     6721 gcgtgtcgct gctgctcgaa gtgctgcgcg gttgcaaagg cagcgtcagg ctgaccggca
     6781 ttcccgaatc cgtgcgcgcg ctgtccgaac tgtacgaaat caaagactgg ctgaaatcat
     6841 gaaaaaaacc gcctatgcct tcctcctgct gatcgggttc gcttccgccc ctgcatttgc
     6901 cgaaacccgc cccgccgacc cttatgaagg ctacaaccgc gccgttttca aattcaacga
     6961 ccaagccgac cgctacattt tcgcccctgc cgcgcgcggc taccgcaaag ttgcgccgaa
     7021 acccgtccgc gccggcgtgt ccaatttttt taacaacctg tgcgacgtgg tcagcttcgg
     7081 tagcaatatc ttgcgcttgg acatcaaacg cgcaagcgaa gaccttgtcc gcgtcggcat
     7141 caacaccact ttcggtttgg gcgggcttat cgacatcgcc ggcgcgggcg gcattcccga
     7201 caataaaaac accttgggcg acacgtttgc ttcgtgggga tggaaaaaca gcaattattt
     7261 cgtgttgccc gtcttagggc cgtccaccgt ccgcgacgcg ctcggcacgg gtattacctc
     7321 cgtttattcg cccaagaata tcgtcttccg cacccctgtc ggacgctggg gcacgactgc
     7381 cgtatccgcc gtcagtacgc gcgaaggtct gctcgatttg accgacagtc tggacgaagc
     7441 cgccatcgac aaatacagct acacgcgcga cctctatatg aaagtccgtg cgcggcagac
     7501 cggtgcaaca cctgccgaag gtacggaaga taacatcgac atcgacgaat tggtcgaaag
     7561 tgccgaaacc ggcgcggcgg aaactgccgt tcaagaagat tccgtatccg aaacacaggc
     7621 agaagcagca ggggaagccg aaacgcaacc tggaacacaa ccttaaagcc atgccgtctg
     7681 aaaatccgtg ttcggacggc attcccttct gtcggaacgg acagtccgaa accacagatt
     7741 gggctataat ctcttttttg cgcataccgc gcgacagcaa aggaaaaacc atgtacgaag
     7801 tcaaccgcag cgtattcgtc ctcatccctt tagaaccttt ttggaactgg ctgcaaaccc
     7861 tgccgggcaa ccaccttgac ggactgaccc tcgaagacat ccaagccgac gcgaattcct
     7921 accttgtccg cccgtgcgaa accgccgacg aagtatggga cgaaatcgaa acccgttttg
     7981 aagacatttt cgccgccgaa cttgccgact ggtgcgaaga cgaacgcgag tggcctgcgc
     8041 tcgatgccga cattttcaac gaatggttcg acatccagct ttctaccgtc atcaccgacc
     8101 tcgaacacga accgcttgcc cgcgaagcct tccaacccat caacctgaac tgatgaagct
     8161 gaccgtccgc aactaccatc tcgacggcta cggccatgtc aacaatgcgc gctacctcga
     8221 atttttcgaa gaagcgcgct gggcgttttt tgaagaacgc ggactgctgc acgagttggc
     8281 aggcctcata ctgattgtcg cccgcatcga catccgatac agccgccccg ccgtcgaagg
     8341 cgacgtattg cagttttcat gccgcctgaa aacccccggc acgcgccgta ttgtgctgac
     8401 ccagacgatt acactgccaa acggcaaaac cgccgcagaa gccgacatca ccctgatgcc
     8461 cgtccacgcc gccacacaac gcaccgtcag cctgcccgcc acccttgccc gcgcattgga
     8521 agccttgtcc gaatgaaaaa aatactcacc gccgccgtcg tcgcactgat cggcatcctc
     8581 cttgccatcg tcctcatccc cgacagcaaa accgcgcccg ccttctccct gcccgacctg
     8641 cacggaaaaa ccgtttccaa cgccgacctg caaggcaaag taaccctgat taatttttgg
     8701 tttccctcct gtccgggttg tgtgagcgaa atgcccaaaa tcattaaaac ggcaaatgac
     8761 tataaaaaca aaaacttcca agtccttgcc gtcgcccagc ccatcgatcc gatagaaagc
     8821 gtccgccaat atgtcaaaga ctacggtttg ccgtttaccg tcatgtatga tgcggacaaa
     8881 gctgtcggac aggcgttcgg cacacaggtt tatccgactt ccgtccttat cggcaaaaaa
     8941 ggcgaaatcc tcaaaaccta cgtcggcgaa cccgatttcg gcaaactcta ccaagaaatc
     9001 gataccgcgc tggcacaata gcgatgccgt ctgaggcgca aaaaaaacag aaacgatgtt
     9061 cctgtttttt tagatcatcc gccccgacac gttcagacgg cattcaacct tccggcagat
     9121 tctttttcac cactgccgga ttgcctgccg ccaaagagta gggcggaatg tcttttgtaa
     9181 ccaccgcgcc tgcgcccacg accgaaccgc gtccgacggt tacgcccgcc attacaatca
     9241 ccctgtgccc cggccagaca tcgtcctcca acgtaatcgg acggatttcc gtgtagccct
     9301 caaagcgttt tttgaacggt caaacttgtg gttatttgaa taaaacagac attccggctc
     9361 catcatcaca tttttgccga ccaccagccc acggcagatt tcacagtttg ccccgatgcc
     9421 cgagccgtcg cccaaaaccg tatccggaaa cacatacgcc ccgcgttcga tattgacccc
     9481 gcgtccgata tgcggagaaa cccgccgcgc caaaaaaccg cgcacccgtc tgccgacgat
     9541 gccgatgccg cgcatatgtg acggcggcaa ccccgaaccg gcaaaacgca gcagcaggaa
     9601 aaagaatttt gatcccatca aacctccctc aaaagaactt tttatagtgg attaacaaaa
     9661 attaggacaa ggcaacgaag ccgcagacag tacagatagt acggcaaggc gaggcaacgc
     9721 tgtactggtt tttgttaatc cactatacat tttatcgaat atactcttga cgtaacaaca
     9781 gaaaatagag agaatgtcgg acttgtctgt agcataggta catcccctat gcccgctttt
     9841 ttgaaaggaa atcaaatgaa acaaggtatt cacccgaact accacgaagt caacgttacc
     9901 tgctcttgcg gcaacaaatt cgcaaccaaa tccgcgatgg aaaaagaaaa cttcaatatc
     9961 gaggtttgct ccctgtgcca cccgttctat accggcaccc aaaaaatcgt tgataccacc
    10021 ggccgcgtgg acaaattcaa caacaaattc ggcaacctgt tcaaacgctg attgccgctt
    10081 tgcaagaatt aagccctgcc atcatttcga tggcagggct ttttaatatt tggcacactg
    10141 acttcttatc tatatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag
    10201 tacagatagt acggaatcga ttcacttggt gcttcagcac cttagagaat cgttctcttt
    10261 gagctaaggc gaggcaacgc cgtactggtt tttgttaatc cactatattt tgacggggtt
    10321 gaagttgaca tctacaaggt ttaatgtttg aatacattca aaaatatatt tgaatgagat
    10381 gttttatgat tcaaaatgtc gttacttcaa taatcctgta ttctgggaca gccgtagact
    10441 tacttattat cctaatgtta ttttttgcca aaagaaaaag cagaaaagac atcattaaca
    10501 tctatttagg acaatttcta ggctctgtta gcctgatatt gctaagtttg ctttttgcat
    10561 ttgtcttaga ttatattcct agtaaagaga ttttaggttt gctcggcttg attccgattt
    10621 tcctaggcct caaagttttg cttttagggg attctgatgg agaggctatt gccaaagagg
    10681 gtttgcgcaa agataataaa aacctgattt ttctagtcgc tatgattact tttgcaagtt
    10741 gtggtgctga caatattggt gtctttgtcc catattttac taccttaaat ttagcgaatt
    10801 tgatagtggc tttacttacc tttctagtca tgatttatct cttggttttt tctgcccaaa
    10861 aattagcaca agtcccttct gttggagaaa ctttggaaaa atatagcaga tggtttgttg
    10921 ctgttgttta tttaggattg gggatatata tcctggttga aaacaacagt tttgatatgc
    10981 tatggactgt gttgggctag gaaaaaatat tatgaaaaag atagtatctg caaagactgc
    11041 catgtgcagt cttttttgtt gccggtcttt ttgtgtctga tgccgtctga agcagtctct
    11101 gcacgacctt tgtgcgaata tttgctacac ttggcaatcc gtccattttt tctcacgctt
    11161 atgctgacct ataccccgcc cgatgcccgc ccgcccgcca aaacccacga aaagccgtgg
    11221 ctgctgctgt tgatggcgtt tgcctggttg tggccgggtg tattttcaca tgatttatgg
    11281 aatcctgccg aacctgccgt ctataccgcc gtcgaagcac tggcaggcag ccccaccccc
    11341 ttggttgccc atctgttcgg tcaaaccgat ttcggcatac cgcccgtgta tctttgggtt
    11401 gccgccgcgt tcaaacattt gctgtcgccg tgggctgccg actcatacga tgccgcacgc
    11461 tttgcaggcg tgtttttcgc cgttgtcgga ctgacttcct gcggctttgc cggtttcaac
    11521 tttttgggca gacaccacgg gcgcagcgtt gttttaatcc ttatcggctg tatcgggctg
    11581 attccgaccg tacactttct caatcccgct gccgccgcct ttgccgccgc cggactggtg
    11641 ctgcacggct actcgctggc gtgccgccgt gtgattgccg cctcttttct gctcggtacg
    11701 ggttggacgc tgatgtcgtt ggcagcagct tatccggcag catttgccct gatgctgccc
    11761 ctgccagtgc tgatgttttt ccgtccgtgg caaagcaggc gtttgatgtt gacggcagtc
    11821 gcctcgcttg cctttgccct gccgctgatg accgtttacc cgctgctctt ggcaaaaacg
    11881 cagcccgcgc tgttcgcgca atggctcgac gatcacgttt tcggtacgtt cggcggcgtg
    11941 cggcacgttc agacggcatt cagtttgttt tactatctga aaaacctgct ttggtttgca
    12001 ctgcccgcgc tgccgctggc ggtttggacg gtttgccgca cgcgcctgtt ttcgaccgac
    12061 tgggggattt tgggcgttgt gtggatgctt gccgttttgg tgctgctcgc cgccaatccg
    12121 cagcgttttc aggacaacct cgtctggctg ctgccgccgc ttgccctgtt cggcgcggcg
    12181 caactggaca gcctgaggcg cggcgcggcg gcgtttgtca actggttcgg cattatggcg
    12241 ttcggactgt ttgccgtgtt cctgtggacg ggttttttcg ctatgaatta cggctggccc
    12301 gccaagcttg ccgaacgcgc cgcctacttc agcccgtatt atgttcctga tatcgatccc
    12361 attccgatgg cggttgccgt actgttcaca cccttgtggc tgtgggcgat tacccggaaa
    12421 aacatacgcg gcaggcaggc ggttaccaac tgggcggcag gcgttaccct gacctgggct
    12481 ttgctgatga cgctgttcct gccgtggctg gacgcggcga aaagccacgc gccggtcgtc
    12541 cggagtatgg aggcatcgct ttccccggaa ttaaaacggg agctttcaga cggcatcgag
    12601 tgtatcgaca taggcggcgg cgacctgcac acgcggattg tttggacgca gtacggcaca
    12661 ttgccgcacc gcgtcggcga tgtacaatgc cgctaccgta tcgtccgctt gccccaaaat
    12721 gcggatgcgt cgcaaggctg gcagacggtt tggcagggtg cgcgcccgcg caacaaagac
    12781 agtaagttcg cactgatacg gaaaatcggg gaaaatatat agaaaacaac agattgagcc
    12841 gaatttctgg attaagtgcc ggaaatcgcg tataattgcg cgattaaac
//
LOCUS       DAXHTY010000056        12868 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXHTY010000056 DAXHTY010000000
VERSION     DAXHTY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.056"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(55..363)
                     /locus_tag="ACFNMK_001329"
     CDS             complement(55..363)
                     /locus_tag="ACFNMK_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364595.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(347..649)
                     /locus_tag="ACFNMK_001330"
     CDS             complement(347..649)
                     /locus_tag="ACFNMK_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC6364596.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(723..3086)
                     /gene="pheT"
                     /locus_tag="ACFNMK_001331"
     CDS             complement(723..3086)
                     /gene="pheT"
                     /locus_tag="ACFNMK_001331"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC6364597.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFTG
                     VVIAEVKSVEKHPDADRLNVTQVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSVKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRVREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAMERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRSRFAVYNEMAAHGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRLVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRVVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3122..3640)
                     /locus_tag="ACFNMK_001332"
     CDS             complement(3122..3640)
                     /locus_tag="ACFNMK_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364598.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3647..4711)
                     /locus_tag="ACFNMK_001333"
     CDS             complement(3647..4711)
                     /locus_tag="ACFNMK_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6364599.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4888..5880)
                     /gene="pheS"
                     /locus_tag="ACFNMK_001334"
     CDS             complement(4888..5880)
                     /gene="pheS"
                     /locus_tag="ACFNMK_001334"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC6364600.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6226..6585)
                     /gene="rplT"
                     /locus_tag="ACFNMK_001335"
     CDS             complement(6226..6585)
                     /gene="rplT"
                     /locus_tag="ACFNMK_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC6364601.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6598..6795)
                     /gene="rpmI"
                     /locus_tag="ACFNMK_001336"
     CDS             complement(6598..6795)
                     /gene="rpmI"
                     /locus_tag="ACFNMK_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC6364602.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6942..7463)
                     /gene="infC"
                     /locus_tag="ACFNMK_001337"
     CDS             complement(6942..7463)
                     /gene="infC"
                     /locus_tag="ACFNMK_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC6364603.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7481..9394)
                     /gene="thrS"
                     /locus_tag="ACFNMK_001338"
     CDS             complement(7481..9394)
                     /gene="thrS"
                     /locus_tag="ACFNMK_001338"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC6364604.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMSEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHSKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLGAEYVTENNDRSRPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9448..9524)
                     /locus_tag="ACFNMK_001339"
     tRNA            complement(9448..9524)
                     /locus_tag="ACFNMK_001339"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9488..9490),aa:Val,seq:gac)
     gene            9705..10820
                     /gene="tgt"
                     /locus_tag="ACFNMK_001340"
     CDS             9705..10820
                     /gene="tgt"
                     /locus_tag="ACFNMK_001340"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC6364605.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQAKKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQAQFHENRARGTD"
     gene            10830..11912
                     /gene="hemH"
                     /locus_tag="ACFNMK_001341"
     CDS             10830..11912
                     /gene="hemH"
                     /locus_tag="ACFNMK_001341"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC6364606.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            12103..12504
                     /locus_tag="ACFNMK_001342"
     CDS             12103..12504
                     /locus_tag="ACFNMK_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6364607.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(12567..12857)
                     /locus_tag="ACFNMK_001343"
     CDS             complement(12567..12857)
                     /locus_tag="ACFNMK_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364608.1"
                     /translation="MSKYVTETDIGVREHFDFFGEFVVSPAARSDDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEQVEGGRIDIGGFMLTAKEVDGDGNIGSMGLKVLR"
BASE COUNT         3267 a         3412 c         2856 g         3333 t
ORIGIN      
        1 ttatttttta gcgtgtaaaa caaaaccggc tgcatacctg caaccggcct caaatcagca
       61 caattcctta tccaaatccg ccaacagctc tttcagaccg tctccgattt cctgaagcgt
      121 aaccggacgg ttgccgtccg aacccgactc cgcaccttct gcatacggtg caaacgtgct
      181 ctgcaatttc aacagtttca ccacatccaa acgggtgtag cgttcgccgc cgtaaccgac
      241 aaccacaccg tgatcatgct gccattgcgc aaaaccatag gggctgattt gcaacagtcc
      301 gcacaactcg tccagcgtga aatagcgttt tgcgggaacc ttcaaatcaa cgttgtttgt
      361 catagtagtg ttccaccatg cttttgagtt tctggctggc atggaaagtt accacgcggc
      421 gggcggtaat cggcacttcc tcgccggttt taggattacg acccgggcgt tgcggcttgt
      481 cgcgcaactg gaaatttccg aaaccggaaa ttttgatttc ttcgccgctt gccaaagtgc
      541 tgcggatttc ttcaaaaaag agttcgacga tttctttggc atcgtttttg gtgacgttgc
      601 tgactttgtc taccaaaata tcggccagtt ctgctttagt gagagtcata tgattacctt
      661 ctgttgtaac agttgtggat tattaccaat ttttatttaa aattcaagcg aatatttatt
      721 ttttaactgc gaagccgcgc ccctgccgcc gttgccgcgc caatcagttt tccgataagc
      781 ggctcgactg cctcatccgt cagcgtgttt tccatatcct gcaaaatcac tttgaccgcc
      841 acgctcttca tcccttcggg cagtcccgtg ccgcgataca cgtcaaacac gctgatttcc
      901 tgtaccaact tgtttgccgc gcctttcaag acaagcagca aatcatcatg gctcatagct
      961 tccggcatca caaacgccaa atcgcggcgc accggctgga atttcgatac gacccgatag
     1021 cgcgttttcc cgcattccaa cacggccgcc atatcgattt caaataccag cggcgcttgc
     1081 ggcaggtcgt atttttgcag ccatttcgga tgcagttcgc cgacaaagcc gatgactttg
     1141 ccgtctgaaa cgatattggc ggcacgtccg ggatgcaggg cgggatgtcc ggttttaacg
     1201 aactcgactg ctttgttttt caacagattt tccacatctg ccttaatgtc gtaaaaatcc
     1261 gcattgcgcg ttttcccacc ccattgttcg ggcattgccg cgccgtacca cagaccgccg
     1321 atgcgttcgt tttggacaag ccggccgtct gaacctttgc cgaacacgcg ggcgatttca
     1381 aacacgcgca cgcggttttg tttgcggttc aggttgtttt gcaaaatttc caccaagccg
     1441 ccgatgagtg tggaacgcat cacggcatat tgcgccgcca gcgggttttg caggcggatg
     1501 gggtcggcgt tggcggcaaa atcttgttcc cactgctcgt caacgaaggc atagctgacc
     1561 acttcgcggt agccgtgcgc cgccatttcg ttgtaaacgg caaaacgcga gcggcgtgtt
     1621 tcgggcagtt ccagcatttt cagacggcct gacgtgtaat cgtcggggat gttttcatag
     1681 ccgtaaacgc gtccgatttc ttcaatcaaa tcggcctcaa tttcgatgtc aaaacggaag
     1741 ctcggcgcgg taacgcggaa gccttccgcc gttttctcgg gctgcaggcc caagtgttgc
     1801 aaaatggttt ccacctgttc ggcaggaatg tccacgccca acacggtttt cagacggtcc
     1861 aaacgcagtc cgacctgctt ggcttcgggc aatttacctt gtgcttcaac catctcgcct
     1921 gccgcaccac cgcaaatctg caacaccaat tcggtagcac gttccatggc atccgcctgc
     1981 aaacggtaat ccacgccgcg ctcgaagcgg aacgacgaat ccgaaccgaa accgtattgg
     2041 cgcgatttgc cggcgatgat ttcgggcgca aaccaagccg cttccagcac gatattttgc
     2101 gtgccgtctg aaaccgcgct cgccgcgccg cccattaagc ccgccaaact caacacgcct
     2161 ttttcgtccg ccacgaccag cgtgttttca gacagggaaa cggttttctc gttcaggcat
     2221 tccagcgttt ccccttcgcg cacgcggcgg atgtgcaggc tgccggaaag tttgtcggca
     2281 tcaaaaacgt gcatcggctg accgatttcc agcatcacat aattgccgat gtccaccagc
     2341 gcggaaatac tgcggatgcc gctgcgctcc aaacgttgtt tcatccaatc gggagtagcc
     2401 gctttcgcat tgacattttc aataacgcgg ctgataaaac ggccgcaatc ggcaggcgcg
     2461 ttaatctgca cgggctgttt tcgactgccc gtgatcggcg cggtatggat ttcgggctgc
     2521 ctgaacgcgc accccgtcaa tgcggacact tcgcgcgcaa tgcccttaac actcaagcaa
     2581 tcagcgcggt taggcgtaat tttcaacgta aacagcgtat cgtccaaatc caagtattca
     2641 cggatattgg taccgacggg cgcgtcttca ggcagaatgt gcaggccgtt cacaccgtcg
     2701 tcgggcagac cgagttcgtc ggtggaacac aacatcccgt ccgacaccac gccgcgcatt
     2761 ttggtcggct tgattttgaa attacccggc aaaacggcac ccggcagcga acacggcact
     2821 ttgatgcccg ctttcacatt cggcgcaccg cacacgatct gaaccaaccc acccgtaccc
     2881 gcatcgactt gggtaacgtt caaacggtcg gcatcaggat gtttttcaac ggatttcact
     2941 tcggcaatta ccacacccgt aaacgcaggc gcggcagtct cggcttcttc cacttccaag
     3001 ccggacatgg ttaacagatg ttccagctta tcggaggaaa gttcggtatc ggcttgggtt
     3061 ttcagccatg agtaggagaa ttgcatgtta tgttctcaat taactaatta actttagatt
     3121 ttcaagagct agctaaacct ttttgaatgg ctaagtccct attaattact tctacagaaa
     3181 tgttccataa agaaatcaac cgcaaagtat ctttcttatt aaattcttgc tttattaatt
     3241 cattcaattg cccaagataa ttaagcggca catccgggat aaccagtaat aatttgattt
     3301 ctatgctacc ccattgcaaa tttgaaaaag cactaggcat aattgtttct ttgatatctt
     3361 ctctgcctaa tattcccaaa atttcaacat ttaatgcatt acaccatttt tgataaatct
     3421 ctgaaatata ttcatcaaaa cgctcaggag atctttcttg atttggaata ctttgttttg
     3481 cctcaacaaa taacaaatta tcttcttgca gatagataaa ctctactgtc ttaatacctt
     3541 cttgccttct cttccggata tatggttcta tataaaacag gttatttttt actagattaa
     3601 aagttatacc tgattcagtg agatttatta tttcactcat aaaaacctaa ccaatccatg
     3661 ctgtttccgg agataaaaca cctatttctt ctctgaacaa tcgaatagac tcaccaataa
     3721 tttcattatc cggcatgcta ttttgtaaat tttcctgcac ccaagtagtc tgttcctttt
     3781 ctttgccagg cataaaaaca ggaatacttt cactatgctt tctagctaat aaacacagct
     3841 tcttgataac aaaatatgaa tgcgtactca taaaaatctg tattcccaat ttggatttag
     3901 ataaggaaaa cagaatttct agaaattgat taattgcttt cggatgtaga ttagcttctg
     3961 gctcatcaat aaataagatt gatccaggac tcaaaaaccg atttcctaat agagtgtcta
     4021 atatcccaat cttttttacc ccctctgctg tagaattgat agagaaagtt tgtctatctt
     4081 tttcatatac ccatgccttc tttttatcat tgtagctaac ataaccgcca aacaatgctt
     4141 ctagatcctt tctagcttcc gctgcctcag ggtaattcct cccttttgtt ggtttaatat
     4201 ccaatgcttt taccaaatca atgtaagtag catcaaaacc aaatctcttt tcttcctctg
     4261 attttttaat tacatcaaat agtgatagaa cttctttggg tggtaaaaaa atagagttag
     4321 cgtttattct gggagaaagt tcgtatagct ccggtttaat tagctttttg gtagttctac
     4381 caaaagaaaa caaaactgaa gaactatctt ctaaagtcat acttaactta agaggccgtt
     4441 cgtttggatt ttcttttcct cttgtaacta aatcaccaat ttcctctact tggaacgtcc
     4501 aatatagctt atctcgcaat tcctcagaaa aatcacgaat attctttcct ttatgagctt
     4561 cctcatgact ttttaaaaca ctatataatg cctttaaaat aaaagttttc cctttgctat
     4621 tttctgcaat aattaaattt attttcccta aattatcagc cttcaataag ctaatcggcc
     4681 cgaaattttc aagccaaaat gatttaatca tataatcccc ctaatatata tcaacaatat
     4741 ttgtacaaga cacattgtga aaagacagca accacagcac catagtaaac gcatagcagg
     4801 cggataatgg agcgacatag cgtttcatga tttagctttc tgaggtaggc acaggctgct
     4861 tttttcattt ttaggcagcc tacacaatca ttttacaaac tgcttcaaaa aattcaaatc
     4921 gttatcaaaa aacaagcgca aatcgttcac gttgtaacgc agcatcgcga aacggtcgag
     4981 accgataccg aaagcgaaac cggtgtattt ttcgggatcg atattcacat ttctcaacac
     5041 gttgggatgc accataccgc agccgccgac ttccagccat ttgccgtttt cgcccataat
     5101 gtcgatttcg gcagacggtt cggtgaacgg gaagaaagac gggcggaaac gtacttgcaa
     5161 atcatcgcgt tcaaagaagc gacggataaa atccgtgaac actgctttta agtcggcaaa
     5221 agttacgccc tcttctaccc acaaaccttc cgcctgatgg aacataggcg agtgcgtggc
     5281 atcgctgtcc acacggtaaa cgcggccggg ggcgataatg cggatgggcg gctctttttt
     5341 atcgagcata tagcggattt gaatcgggga agtgtgcgta cgcaaaacat caccgttttc
     5401 aacgtaaaac gtatcctgca tcgcacgggc aggatggttt gcagggatgt tcagggcttg
     5461 gaaattgtga aaatcgtctt cgatttcagg cccgtccgcc acttcgaaac ccattccgtg
     5521 aaagagttcg accacacgtt gcaaggtcag ggttacggga tgcaggctgc cgccttcctg
     5581 agcgcgtccg ggcagggtaa tgtcgagggc ttcggcggca agtcgggctt gcagcttggc
     5641 ttcgttgagg gcatcgcgtt tggcattaaa agccgtctga aaccggtttt tgcattcatt
     5701 gatatgcgca cctatggttt tgcgctcttc aggcgacatt tgccccaaag ttttcagaag
     5761 tccggtcaac tcgccggttt tgccaagata acgggctttg atttgttcta gagcgttgaa
     5821 gtcttgcgca gcttctactg cggcaatgcc ttctgcaacg atgcggttta cattttccat
     5881 aatatcaagt ctgtcaatta atgatatgcc acctgaaaca acgaggccgc ctgtatagcc
     5941 tgacatatca gactcttcag acgaccttat tttcttcacg gtcaatccat tggataaatt
     6001 ttctgtatag tggattaaat ttaaaccagt acggcgttgc ctcgccttgc cgtactatct
     6061 gtactgtctg cggcttcgtc gccttgtcct gatttaaatt taatccacta tattttaacg
     6121 caaatttatt taaaaaaata gactttaaag aaagaaaatc aatcacatag aatttctgca
     6181 aacaaagaaa aaaggaagcc gcagcttcct tttcaatttt ttggattaag cagccaaagc
     6241 agctttggct ttttcaacca attgtgcaaa agcggcttta tcgaacacgg ccaaatcagc
     6301 caatactttg cggtcgattt cgatagaggc acgtttcaga ccattcataa atttgctgta
     6361 agacaacccg ttttcacgcg cacctgcatt gatacggaca atccacaatt gacggaattg
     6421 gcgtttgcgt tggcggcggt cgcggtatgc gtattggccg gctttcatta ccgcctgctt
     6481 ggcaacgcgg taaacgtttt tacgacggcc gcgatagcct ttggctaacg cgaagatttt
     6541 ttggtgacgg gcacgggcgg taacaccgcg ttttacgcgt ggcatattct aaactcctta
     6601 agcgtagggt aacattttag caacagaagc caaatcgcga tcatttacca tagaggtacc
     6661 gcgcagttgg cgtttgtttt tggtggtctt tttagtcaag atgtggcgtt tgaacgcatg
     6721 agcgcgtttc acaccgccgt tacccagtac tttaaagcgt tttttcgcgc tagacttggt
     6781 tttcatttta ggcatgggaa aactccattc gttatcggat aaggcattag ggggttttaa
     6841 aacatcggtt tcaaacactt gaacccacaa tgccacggtt ttttgccgca attgaaaact
     6901 tactccgaag cggcaatcgg agtaagcgga gaattatagc tttatttttt cttcggcgca
     6961 atcatcatca ccatttggcg accttccatt ttgggaaagg actcgatttg cgccacttca
     7021 gccaaatctt ctttcacacg ttccaaaagt tgcgcgccga gttgctggtg agccatttca
     7081 cggccgcgga aacgcaatgt cactttgact ttatcgccgt cggcaaggaa gcggttgatg
     7141 ttgcgcatct tgatttgata atcgccctca tccgtacccg gacggaattt gatttccttg
     7201 atttgcacct gcttttggtt tttcttggct tcgtcgcgtt tcttggcctg ctggtatttg
     7261 tatttaccgt aatccatcag tttgcacaca ggcggtttag cagttgggga aatctctacc
     7321 aaatcgacat cctgcccttc ggccatagcc aaagcttcac gaactgaaac aacaccaagc
     7381 tgttcgcctg actcactgat taaacgcact tctttggcgg taatttcgcc gttgattcgt
     7441 gcttcgcgtt cttgagcgat gatgaatact cctataaaaa ttaatgattg acgagggcat
     7501 cagtgatttc ttgctgcaat tgcgcaatga aatcatccaa atccaaagaa cccaaatctt
     7561 ctgctttgcg gcgtaccgcc actttgtttt cctgcttctc cttatcgccg acaacgattt
     7621 gataagggaa acggtattgg ctgttgtcgc ggattttgta accgattttt tcgttacgca
     7681 aatccaactc ggcgcggaat cctgccgcct gcaatttggc agccacttcc cgacaataat
     7741 ctgcctgatt ttcggtaata ttcataatca ccatttgaac cggagccaac cataacggga
     7801 aagagcctgc atggttctca atcagaatgc cgataaatcg ctccaaagaa cctaaaatgg
     7861 cgcgatgcaa cataacaggt cgcgagcggt cgttgttttc ggttacatat tctgcaccca
     7921 agcgttccgg caagacgaaa tccagttgta atgtaccgca ttgccaagaa cgacccaagg
     7981 catctctgac atgatattcg attttaggcc cgtaaaacgc accctcgccc ggcaattcgc
     8041 cccattccac gccgcaggca gtcaatgcct cgcgcaaacc ctgctctgcc ttatcccaca
     8101 cgtcatctga acctgcacgt ttttcagggc gaagagaaag cttgacggat acatcatgga
     8161 aaccgaactg tttgtagatg cgaaccaaca attcattgaa cgcacgggcc tcgctgacga
     8221 tttggtcttc ggtacagaaa atatgcgcat catcctgcac aaatccacgg acccgcatca
     8281 gaccatgcaa tgcaccgctc ggctcgttgc ggtggcagga accgaattcc gccaaacgca
     8341 tcggcaaatc tcgatacgaa cgcaaaccgt tgttaaaaat ttgaacatga cccggacagt
     8401 tcatcggttt aaccgcatat tcgcgttttt ccgaactggt tacgaacata ttatctttgt
     8461 agttgtccca atggccggat ttttcccaaa aggttttatc catgatttga ggcgttttga
     8521 cctctttata accggcggcg ttcagctctt tacgcatatg ctgttcaatc acttgccaca
     8581 aagcccagcc tttagaatgc caaaacacca tgcccggcgc ttcgtcttgc aggtggaaca
     8641 aatccaattg cttgcccaat ttgcggtggt cgcgcttttc ggcttcttcg atacgttgaa
     8701 tataggcttt caactcgtct tttgtcgccc aagccgtacc gtatatgcgt tgcagcattt
     8761 cattgttgct gtcgccgcgc cagtatgcgc ccgccaactt ggtcagtttg aagtttttca
     8821 ggaaacgggt attcggaacg tgcggaccgc ggcacatatc gacatattcc tgatgatgat
     8881 acatccccat cgcttccact tcggacatat cgtcaatcag gcgcagtttg tattcttcgc
     8941 cgcgctcttg aaaaatttta atcgcctccg cacgcggagt catgattttg accacatcat
     9001 agtcttgggc aatcaattct ttcatacgcg cttcaatggc ggcaacatct tccggtgtaa
     9061 acggtttttc cgtggcgatg tcgtaataaa agccctcttc aatgacgggg ccgataacca
     9121 tttttgcatt aggatagagt tgcttgacgg catgcccgac aagatgcgcg caggaatggc
     9181 ggatgatttc gatgccttcc tgatctttcg gagtaatgat ttgaacagca gaattttcaa
     9241 caatcgggtc gcacgcatcg accaatttgc cgtttaccct gcctgccacc gccgccctcg
     9301 ccaaaccgac accgatagac gcagcaattt gagccacggt aacgggggat tcgtattggc
     9361 ggactgagcc gtccggcaag gtaatgttca acatcaaaaa gctccaaaac attaaaaaat
     9421 aacggcataa atgccgttat gggaatttgg taggcacgat tggattcgaa ccaacgaccc
     9481 ccaccatgtc aaggtggtgc tctaaccaac tgagctacgt gccttctaaa gaatttttgc
     9541 attttatcgg gtcgttttaa tttttgcaag aggtatgtgc atttctttat gcaacattgt
     9601 ggcaagattt ggggatgtcg tctgaaaaac cgtgggaatt catttataat gccgtctttt
     9661 aaaacatatt tcgcccccct gtcgcccaac tgacattcaa tcttatgctt aaatttacct
     9721 tacacaaaaa agacggatac gcccgacgcg gcacgctgga gttgaaccac ggcaaaattg
     9781 aaacgccggt atttatgcct gtcggtactt acggctcggt taaggcgatg aacccgcaaa
     9841 acctgcacga catcaaggcg caaattattt tgggcaacac ttatcatttg tggctgcgtc
     9901 cgggcttgga ggtcgtcgaa cagtttggag ggcttcacgg ctttatcggc tgggacaaac
     9961 cgattctgac cgactcgggc ggttttcagg ttttttcttt gtcggatatg cgcaagctga
    10021 cggaagaagg ctgtacgttc aaaagcccga tcaacggcga caagctgttt ttatcgcctg
    10081 aaatttccat gaaaatccaa acggttttga actcggacat cgcgatgcag ctggacgaat
    10141 gcacgccggg cgaagcaacg cgcgaacagg cgaaaaaatc gctgcaaatg agcctgcgct
    10201 gggcggaacg gagcaaaaaa gcctttgaag atttgaaaaa cccgaacgcg ctgttcggca
    10261 tcgtgcaagg cgcgatgtat gaggatttgc gcgaagaatc gctgcgcggt ttggaacagt
    10321 ttgattttcc cggccttgcc gtcggcggct tgtccgtcgg cgaacccaag cccgaaatgt
    10381 accgtatgct gcgcgccgtc ggtccgatac tgcctgaaca caaaccgcat tacctgatgg
    10441 gcgtaggcac gccggaagac ctcgtgtacg gcgtggcgca cggcatcgat atgttcgact
    10501 gcgtgatgcc cacccgcaat gcacgcaacg gctggctgtt cacccgtttc ggcgatttga
    10561 aaatcaaaaa cgccaaacac aagctcgaca agcgtccgat agacgaaagc tgcacctgct
    10621 acgcctgcca aaacttcagc cgcgcctacc tgcaccatct gcaccgcgcc ggcgaaatct
    10681 tgggcgcaca gctcaacacc atccacaacc tgcatttcta ccaagtcatt atggcggaaa
    10741 tgcgcgaagc cgtcgaacaa ggcaaatttg ccgactggca ggcgcaattc cacgaaaacc
    10801 gcgcgcgcgg cacagactga ttcccggcaa tgccgtctga agccgcttca gacggcattt
    10861 tgccgcttta ccctacccgc actatttttc agaaaccgat catgcttcca ttccttccag
    10921 aaccctccct ttcctatacg caacaaaacc gtaccgccgt tttgctgttg aacctcggca
    10981 cgcccgacgc gccgaccgcg caggcggtac gaccttatct gaagtccttt ctgaccgacc
    11041 ggcgcgtcgt cgaactgccc aaatggctgt ggtatcccat tctgcatggt ctggtactga
    11101 cgctccgccc caaaaaaagc gcgcacgcct atgaaaaaat ctggtttaag gaaggttcgc
    11161 ccttagaagt ttacacagca cgccaagctg ccgcgcttgc caaacgtatg cccgacctca
    11221 tcgtccgcca cgccatgacc tacggcaatc cttcggtggc agacgtattg tcggaactga
    11281 aggcgcaagg cgcgggcaga ctgctggtca tcccgatgta cccgcaatat gccgcttcca
    11341 gcagcggagc ggcagtagat aaggtgtgcg aacaattact gctgcaacgg aaccaaatga
    11401 gcgtccgcac cgtttcgcgc ttttatgacg ataccggcta cattgatgcg atgaaaaacc
    11461 acatcctccg atattgggcg gaacacggac gtgggaaaaa actgatgttg agttttcacg
    11521 gcgtaccgca gaagcaccac gacctcggcg acccctatcc cgacgagtgc cgccacaccg
    11581 ccaaactgct tgccgaagca ctggaactga ccgaagacca atatgtcgta tcgttccaaa
    11641 gccaattcgg cagggcaaaa tgggttacac cgagcacgca ggatttgttc ggcaaactgc
    11701 ccaaacaagg cgtaaccgaa ctggacgtat tctgcccggg ctttttggca gactgtttgg
    11761 aaaccatgga agaaatcgcc ctgatggggc gtgaacagtt ttacgaagca ggcggcaaaa
    11821 gctaccgcta catcccctgc ctcaacgaca accccgactg gatagacgca ctcgtcgcac
    11881 ttgccgaaga aaaccttggc ggctggcgtt gaaactgttt gacccatgtc taaaatgccg
    11941 tctgaacgcc gtgttcagac ggcattttcc cgctacttta gcgtatgatg gcatctatgc
    12001 tggacagcac atcaagtgca accttaccac cgattcggca aaaaaacctt tcacccgcta
    12061 cgcaaaatca acaacaaggt ttcaaacatt aaggaaacac caatgaacac aacccgactg
    12121 ccgaccgccc tcgtcttggg ctgcctctgc gccgccgctt ctgccgccga caacagcatc
    12181 atgacaaaag ggcaaaaagt gtacgaatcc aactgcgtcg cctgccacgg caaaaagggc
    12241 gaaggccgcg gaaccatgtt tccgccgctc taccgctccg acttcatcat gaaaaaaccg
    12301 caggtgctgc tgcacagcat ggtcaaaggc atcaacggta caatcaaagt caacggcaaa
    12361 acctacaacg gattcatgcc cgcaaccgcc atcagcgatg cggacattgc cgccgtcgcc
    12421 acttatatca tgaacgcctt tgacaacggc ggcggaagcg ttaccgaaaa agacgtaaaa
    12481 caggcaaaag gcaaaaaaaa ctaaacagac aaaatgccgt ctgaaaccgg caatccggct
    12541 tcagacggca ttcaaatcaa acctttctaa cgcagcactt tcagacccat agacccgata
    12601 ttgccgtccc cgtcaacctc cttcgcggtc agcataaagc cgccgatgtc aatacggccg
    12661 ccctcgacct gctcctgact gtcggaacgc ttatcgaaaa gctcggcaag gctcaaaccc
    12721 tcttcgcccg cttccagcct caaaccgtaa gcaagtgcca aatcgtccga acgtgctgcc
    12781 ggcgaaacga caaactcgcc gaagaaatca aaatgctcac ggacaccgat atccgtctcg
    12841 gtaacgtatt tcgacatttt atagtgaa
//
LOCUS       DAXHTY010000057        12596 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXHTY010000057 DAXHTY010000000
VERSION     DAXHTY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.057"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..193
                     /locus_tag="ACFNMK_001344"
     CDS             <1..193
                     /locus_tag="ACFNMK_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364609.1"
                     /translation="IPRVRHAPRSAMIGAVSFESAHRDECPRAVPIPICRLFRIGCRR
                     EMTDGCRNGGRREWEEMTD"
     gene            298..1611
                     /locus_tag="ACFNMK_001345"
     CDS             298..1611
                     /locus_tag="ACFNMK_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC6364610.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1829..2004
                     /locus_tag="ACFNMK_001346"
                     /pseudo
     CDS             1829..2004
                     /locus_tag="ACFNMK_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2000..3250
                     /locus_tag="ACFNMK_001347"
     CDS             2000..3250
                     /locus_tag="ACFNMK_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6364611.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVCKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERVRVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(3285..4778)
                     /gene="rng"
                     /locus_tag="ACFNMK_001348"
     CDS             complement(3285..4778)
                     /gene="rng"
                     /locus_tag="ACFNMK_001348"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC6364612.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            4992..5249
                     /gene="grxC"
                     /locus_tag="ACFNMK_001349"
     CDS             4992..5249
                     /gene="grxC"
                     /locus_tag="ACFNMK_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC6364613.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5270..5713
                     /gene="secB"
                     /locus_tag="ACFNMK_001350"
     CDS             5270..5713
                     /gene="secB"
                     /locus_tag="ACFNMK_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC6364614.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSSTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5799..7841
                     /gene="recG"
                     /locus_tag="ACFNMK_001351"
     CDS             5799..7841
                     /gene="recG"
                     /locus_tag="ACFNMK_001351"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC6364615.1"
                     /translation="MMSPETRKQLKITDVSAKKLEKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            7838..8065
                     /locus_tag="ACFNMK_001352"
     CDS             7838..8065
                     /locus_tag="ACFNMK_001352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364616.1"
                     /translation="MSEMSPEILEKSATESDFALFLTHFHDFKSRYSQVGGNPAMGSR
                     RDLSGKRQALRRHSRAGGNLEMKNHGVIGND"
     gene            8110..8235
                     /locus_tag="ACFNMK_001353"
                     /pseudo
     CDS             8110..8235
                     /locus_tag="ACFNMK_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(8386..9429)
                     /gene="argC"
                     /locus_tag="ACFNMK_001354"
     CDS             complement(8386..9429)
                     /gene="argC"
                     /locus_tag="ACFNMK_001354"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC6364617.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIWDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(9426..9707)
                     /locus_tag="ACFNMK_001355"
     CDS             complement(9426..9707)
                     /locus_tag="ACFNMK_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364618.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            9961..11115
                     /locus_tag="ACFNMK_001356"
     CDS             9961..11115
                     /locus_tag="ACFNMK_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6364619.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDPRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTATQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            11124..11351
                     /locus_tag="ACFNMK_001357"
     CDS             11124..11351
                     /locus_tag="ACFNMK_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364620.1"
                     /translation="MKNWKQFTFFVILVIACYQVLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            11344..11697
                     /locus_tag="ACFNMK_001358"
     CDS             11344..11697
                     /locus_tag="ACFNMK_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364621.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            11758..12384
                     /locus_tag="ACFNMK_001359"
     CDS             11758..12384
                     /locus_tag="ACFNMK_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HFC6364622.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRATNLFIS"
     gene            12288..12536
                     /locus_tag="ACFNMK_001360"
                     /pseudo
     CDS             12288..12536
                     /locus_tag="ACFNMK_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFP transporter"
BASE COUNT         2933 a         3101 c         3549 g         3013 t
ORIGIN      
        1 aatcccgcgt gtacggcacg ccccgcgctc cgcaatgata ggggcggttt cgtttgaatc
       61 ggcgcaccgg gatgaatgcc cccgcgccgt cccgataccg atttgccggt tgttccgtat
      121 cggttgccgc cgtgaaatga cggacggatg caggaatggc ggcaggcgtg aatgggagga
      181 aatgaccgat tgacttgttt caaaataaaa aagtagaatg cagggcttat cccgcgtctg
      241 tgggcggttt tgcctgcacg gtcggttttc tgtttaaaat aaaataaagg agaaatgatg
      301 ctgacgttta tcggactgct gattatcggg gtcatcgtat ggttgctgct gacggaaaaa
      361 gtgtcgccca tcatcgcatt aatcttggtg ccgctgtttg gggcgttgct ggcggggttt
      421 gatgtatccc aattaaaaga attttattcg ggcggcacca aatcggtgat gcagattgtg
      481 attatgttta tgttttccat tttgtttttt ggaatcatga acgatgtggg gctgtttcgt
      541 ccgatgatag gcggtttgat taagctgact cggggtaata tcgtggcagt gagtgtgggg
      601 acggtcttgg tgtcggtggt ggcgcagttg gacggggcgg gtgcgacgac gtttttattg
      661 gtcgtccccg cccttttgcc gctttacaag cgtctgcata tgaatcctta cctgctgttt
      721 ttgctgctga cttccagtgc gggattgatt aaccttctgc cgtggggcgg gccgaccggg
      781 cgggttgcaa gcgtgttggg cgcagatgtg ggcgaattgt ataaaccttt gttgacggtg
      841 caaattatcg gtgtggtgtt tatccttgcg ctatccctgc ttttgggtgt gcgtgaaaaa
      901 aggcggattg tccgggagtt gggcgcgttg cccgccgtgg cggatttgat aaagccggtg
      961 cctttgtcgg aagaagaaca aaaattggcg cgtccgaaac tgttttggtg gaatgtcctg
     1021 ctgtttttgg cggcgatgag cctgcttttt tcgggcatct tcccgccggg ttatgtattt
     1081 atgctggctg caacggcggc gttgcttttg aattaccgca gcccgcagga acagatggag
     1141 cggatttatg cccacgccgg cggcgcggtg atgatggcgt ccattatttt ggcggcaggt
     1201 acgtttttgg ggattttgaa gggtgcgggg atgttggacg cgatttccaa agacattgtg
     1261 catatcctgc cggacgcgct gctgccttat ctgcatattg ccatcggtgt gttgggcatt
     1321 ccgcttgagt tggttttgag tacggacgct tattatttcg gactgtttcc gattgtggag
     1381 cagattacct cgcaggcggg cgtggcgccc gaagcagcag gttatgcgat gttgatcggc
     1441 agtatcgtcg gcacttttgt tacgccgctt tcgccggctt tgtggatggg cttgggtttg
     1501 gcgaaattgt cgatgggcaa acacatccgt tattcgtttt tttgggcgtg gggtttgtcg
     1561 ctggcgatat tggccagttc gatagcggca ggaatcgtgc ctctgccgta aacggcgaag
     1621 ccgtctgaag cctgaatcgt tcctgtggtc tgtcgcaggc tctgcctgcc cgtgtgttgc
     1681 aatcgcgccc caatgccgtc tgaaggcagt tgccggcgcg gttttttgtg ccgcagtttt
     1741 gaaaaatacc ggcaagcgta aaggccgcta aattgcggct aattttgggg tgttagaata
     1801 ggcacggttt attttgaagg gaaagttgat gcgtgttttg ctggtggaag acgatgcgat
     1861 gattgcggag gcggtgtcgg caagtttgaa agacggcggc tatgcggtgg attgggtcaa
     1921 aaacggcgcg cggctgccgc tgcccagcct tatgacttga tgctgctgga tttgggtttg
     1981 ctggggcggg acggtttgga tgtttgttcc gctggcaatg cttgcaggta tgttttccta
     2041 ctacgaaacc ttccacgaaa cggaagcgtt gcaggacgac ctgctccgtc aggcggcatt
     2101 gtacgtcgca cccgattcca aacccgaaac tttgcccgag ggcgacggcg atacgcgcat
     2161 tttcgtgcag atgccgcagc aggaagaccc tgttgtcagc ctgcctgcgc atctggcgga
     2221 cggtctgcac acgcttcagg cggacgggga cgacgattat taccgcgtct atatccgcac
     2281 gaccgaacag ggacggattg ccgtcatgca ggaaaacgaa taccgtgaag atttggcggc
     2341 ggatgcggca cggcaaagcg tgttgcccct gctggcggca ctgccgctga tgatactgct
     2401 gaccgtgtgg attacgcaca aagccatgcg ccccgtctgc aaattgtcgc aaagtctcga
     2461 acaacgccga atcaatgacc tgtctgcctt gagtgtggac aatattccca gtgaaatcag
     2521 agggttcgta accgccatca acttgctttt gaaacgtgcg gatgaagaca tccgccaccg
     2581 tcagcggttt gtcgccgacg cggcacacga attgcgtacg ccgatgacag ccctttccct
     2641 tcaggcggaa cggctcaaca atatgtctct gccacccgat gcggcgcggc agtctgccgt
     2701 tttgcagcaa agcatacggc gcaacaaaca cctgctcgag cagcttttgg cactggcgcg
     2761 ttcgcagtcg gacgaaaccc ctttggcgaa aacgacattc ggtctgcaaa gccgtttccg
     2821 ccaagtgttg caggaactga tgccgcttgc cttggaaaaa cgtcaggaca ttggtgtggc
     2881 ggtcggaggt gatgtcgaaa tatttgccga tgaaactgaa atctatacct tggttaaaac
     2941 gtttgttgat aacgcaatac gttatacccc gtccgaaggc aggatagatt tgggtttcac
     3001 agacgaaggg aaatatcttg ccgtgtgggt ggaagataac gggaacggca ttccggaatc
     3061 cgaacgcgtc cgcgtcctcg atccgtttta ccgtattttg ggaacggagc agcaggggac
     3121 ggggctgggg ctgtcgattg ccgacacgct agccaaaaaa tacggcggat atttggaact
     3181 gaccgacagc cgcaggttcg gacacggtct gctgatacgc gccttgttgg acaaggaaac
     3241 cctgaaatag acagatgccg tctgaaggct tcagacggca ttttttacat cagcacaata
     3301 tcgtattgtt cctgcgtgta agcggtttcg accgccagag aaatcggttt gccgatgaaa
     3361 tctatcagca ttgccaagga ttgcgattct tcgtccaaaa acaaatcgat gacgttgggg
     3421 gcggcgagga tgcggaaact ttcggcatcg taacggcgcg cttcgcggac gatttcgcgc
     3481 tggatttcgt agcacacggt ttgcggcgtt ttcagacggc ctctgccttg gcaggaaggg
     3541 cagggttcgc agaggacttg gtttaagttt tcgcgcgagc gtttgcgcgt cagctcgacc
     3601 agccccaggc tggtaaaacc gtgcagggta acgcgggtac ggtcgaaggc gagggctttg
     3661 gcaagctcct gcaacacggc ttcgcggtgg ctttcctgcg ccatatcgat gaagtcgatg
     3721 atgatgatgc cgccgaggtt gcgtagcctc aattcgcggg cgatggtgtg gcaggcttcg
     3781 aggttggtgc ggaagatggt ttcgtcgaag ttgcgtgcgc cgacgaagcc gccggtgttc
     3841 acgtctatcg tggtcatggc ttcggtggat tcgataatca ggtagctgcc gaagttgagg
     3901 ttgacgcgcg gttgcagggc gcggctgatt tcctgttcga cgttgtgggt ttcaaacagc
     3961 gggcgttcgc ctttgaacaa ttctatcctg cccaatgcgc cgtggacgta ttgttcggca
     4021 aaacgcgtca tgcgcccgtg gtttacggtg gaatcgacga ggattttttg cgtgtcgcag
     4081 ccgaccatat cgcgcaatac gcgcaaactc aagggcaaat cctgataaag cagggtttcc
     4141 ggcggccgga ttttcgcctg ttcttggatg tgttcccaca ctttggtcag gtagtcgatg
     4201 tcggactgga gctgttcgtc ggtggcgttt tcggcgttgg tgcggatgat gtagccccgg
     4261 caggcatttt ccggcaggag cttgtcgagg cgttcgcgca ggctgctgcg ttcggcatcg
     4321 tcttcgatgc gttgggacac gccgatgtgg tcttcttgcg gaagatggac gaggaaacgc
     4381 cccgccagcg agatttgggt ggaaagccgc gcgcctttgg tgttgatcgg gtctttgatg
     4441 acctgcacca aaacagactg cccttcaaac agcatatgtt cgatgcgctg ggtttcttcg
     4501 gggttgcggc gttgttcgag gacatcgacg atgtgtaaaa acgccgcgcg ttccaagccg
     4561 atgtcgataa acgcgctctg catcccgggc agcacgcggc gcaccacgcc cagatagata
     4621 ttgccgacca ggctgtgttc gctgttgcgc tcgatgtgca gctcgcagat attgttttcc
     4681 tccaacaccg ctacgcgcgt ttcctgcggc gtgatgttga ccaatatcgt ttcgggcggg
     4741 cgcgcgatgt ctttggggat ggggagtcct gacaacatgg tttttcctga aaatgtaata
     4801 aaaatatttt tgcacccgtt ccgccgcgca gcgcgggtgc aaaaatattt ttcacaatgc
     4861 ctatcatact ttaaaaaaga aactttgaca cattccgcac atgccgtttg aacgtgcatc
     4921 tgttccgcac ttgccaaaca ggggaaatgc ccctatattg attatcattg caaaactttc
     4981 ggaaacccaa tatgcagacc gttaccatgt acacaggtcc gttttgtccc tactgcgcga
     5041 tggcgaaaag gctgctgcac gcggcaggtg tcggacatat cgacgaaatc cgtgtcgatg
     5101 caagccccga agcctttgcc gaaatgcagc agctttcggg acagcgcagc gtgccgcaga
     5161 ttttcatcgg cgaaacgcac gtcggcggat ttaccgacct ctaccgcctc cagcaggaag
     5221 gcgggctgga cggactgctg aacccttaac ccccaactag gaaaacaaaa tgagcgaaga
     5281 actgcaaccc gtattcagca tcgagcgact gtatgtcaaa gacttgtctt tggaagtgcc
     5341 gcacgcgccg caaatctttt tggaacaggg cgagcctgaa gtggatatgc gcgtttccac
     5401 cggcagccaa aagctggaag acggctacta caacgtggac gttaccgtaa ccgttaccgc
     5461 caagctggat aacgagcgca cgatgttttt gaacgaagta acccaaagcg gcattttccg
     5521 tttggaaaac atccccgaag aagatgtgca gctgctgttg ggcgtggcgt gtccgaacat
     5581 cctcttccct tacgcgcgcg aagccgtttc cagtacggta acgcgcgccg gcttcccgcc
     5641 cgtcctgctc gcgccgatta attttgaggc gatttaccaa caacagcagg aagccgaagc
     5701 cgccggggct tgattcctgc ctgccgatgc cgtctgaatc cgtttcagac ggcatttttg
     5761 ttttcggata aaataggcgc gtccaaagga atctgcctat gatgtcgccc gaaacccgaa
     5821 aacagctcaa aatcaccgat gtttccgcca agaagctcga aaaactcaac ctccataccg
     5881 catgggattt ggtgttgcac ctgccgttgc gttacgagga tgagacgcac atcatgccga
     5941 ttaaggacgc gccgattggc gtgccgtgcc aggtcgaggg cgaggttatc catcaggaag
     6001 taacgttcaa accgcgcaag cagttgattg tccaaatcgc cgacggttcc ggcagcgtcc
     6061 tttttctgcg cttcatccac ttttacgcca gccatcagaa acagacggcg gtcggtaagc
     6121 gcatccgcgc cgtgggcgaa atcaaacacg gcttttacgg cgacgagatg attcatccca
     6181 aaatccgcga tgccgagggc ggcggtttgg cggaaagcct cacgccggtt tacccgaccg
     6241 taaacggttt gaaccagccc actttgcgcc gtattattca gacggcgttg gacgttacgc
     6301 cgctgcacga cacgctgccc gatgccctgc tgtgccgtct gaagctgcca cacctcgccg
     6361 aaagcctgcg tcttttgcat tcgccgccgc cgagtttcac gattcatcag ctttcagacg
     6421 gcacgctgcc tgcatggcaa cggctcaaat tcgatgaact tttggcgcaa cagctttcga
     6481 tgcgcttggc gcgacagaag cgtatcggcg gcacggcggc ggcattgggc ggcgacggca
     6541 cattgaccca agccctgcgc caagccctgc cgtttgccct gaccgatgcg caagaaaaag
     6601 ttgtttccga aatctgccgc gatatggcgc aaacctaccc gatgcaccgc ctgctgcagg
     6661 gcgatgtcgg cagcggcaaa accattgtgg ctgctttgtc tgctttgacg gctatcgaat
     6721 ccggtgcgca ggtggctgta atggcgccca ctgaaatcct tgccgaacag cattttatta
     6781 aatttaaaca atggctcgaa cctttgggca ttgaagttgt ccgccttttt ggcagtttgc
     6841 gtaaaaaagc caaagacgaa gccaaagcca aactcgctga cggcagcgtc aaaatcgccg
     6901 tcggcacgca cgccctgttt tcagacggcg tggcgtttca caatttgggc ttgaccattg
     6961 tggacgaaca gcaccgtttc ggcgttgccc aacgcctcgc cctcaaaaac aaagggcgcg
     7021 aagtccatca gctgatgatg tccgccacgc ccatcccgcg cacgcttgcg atgagttttt
     7081 tcgccgattt ggacgtgtcc gtcatcgacg aattgccgcc cgggcgcaca ccgattaaaa
     7141 cgcgcctcgt caacaacgtc cgccgcgccg aagtcgaagg cttcgtcctc ggcacttgcc
     7201 gaaaagggcg gcaggcgtat tgggtctgcc cattgattga agaaagcgaa accctgcaac
     7261 tgcaaaccgc cgccgaaacc ctcgcccggc ttcagacggc attgcccgaa ctcaatatcg
     7321 gactggtaca cgggcgcatg aaggccgccg aaaaggccga agtgatggcg cggttttctt
     7381 cgggggggct gaacgtcttg gtcgccacca ccgtcatcga agtcggcgta gatgtgccca
     7441 atgccgccct gatggtcatc gaacacgccg agcgcatggg cttggcgcag cttcaccaat
     7501 tacgcggacg ggtagggcgc ggcgcggcgg aaagcgtgtg cgtcctcctg tttgccgaac
     7561 ccttgggcga actcgccaaa gcgcggctga aagtcatcta cgaacacacc gacggctttg
     7621 aaatcgcccg ccaagacctc aacatccgcg gccccggcga atttctcggc gcgcgccaaa
     7681 gcggcgtgcc tatgctgcgt ttcgccaagc tcgaagaaga cctgcacctc ttggaacaag
     7741 cgcgcgaaac cgccccgatg ctgattgaac aaaaccccga aatcgtcgaa gcgcatttgg
     7801 cacgatggct ttccggcagg gaaggttatt tgggcgtgtg agcgaaatgt cgccggaaat
     7861 attggaaaaa agtgcaacgg aatctgattt tgcattattt ttgacacatt ttcatgattt
     7921 taaatctcgt tattcccaag tgggcggaaa tccggcaatg ggaagccgca gagacttatc
     7981 gggaaaacgg caagccctcc gccgtcattc ccgcgcaggc gggaatctag aaatgaaaaa
     8041 ccacggtgtt atcggcaatg actgaaaccg gagaaatgac gaaattgaag cgtgtggaaa
     8101 tgtgtcggaa atcgctgaag ccggataaac tatagtggat taaatttaaa tcaggacaag
     8161 gcgacgaagc cgcagacagt acagatagta cggcaaggcg aggcaacgct gtactggttt
     8221 aaatttaatc cactatagat tcccgctttt gcgggaatga cggtatttta gatttttgtt
     8281 ttcgcggaat aatgagccaa aagccgtccg aacccaaaaa aaccgtcgct ttcagacggc
     8341 atcaaaaaac cgcaggcacg atgcctgcgg tttgccgttt gcgcttcagg ggagcagggg
     8401 gattgcgccc aagccgtgtg tttccttcag tccgaacatg atgttcatat tttgcaccgc
     8461 ctgacccgcc gcgcctttga cgaggttgtc gatgacggaa aggacgaccc acacatcgga
     8521 ttgcgccgcc tgttggatgc tgatgcggca gaggtttgcg ccgcgcacgc tgcgggtttc
     8581 gggtgtcgaa ccggcgggca ggatgtccac gaacaggctg tcgcggtaat agtcgcgcag
     8641 gatggtttcg gggcagatgc cgtctgaaag gtggaggtaa acggtggcgt gcataccgcg
     8701 tatcatcggc gcgaggtgcg gcgtgaacac gaatccttcg gcgatgccgt cctgaagccc
     8761 ggcgatggtc tgcctgattt cgggcaggtg gcggtgtcca gctatgccgt aggctttgaa
     8821 gttgtcgccg acttcgcaca acagcgaacc gacattgcct ttcctgcccg cgccggacac
     8881 gccggatttg cagtcggcaa tcagcggcat accgggcttc agacggcatt gccgcaacag
     8941 cggcacgagc ggcagggata cgcaggtcgg gtagcagccg gggttggcga cgaggcgcgc
     9001 ctgtgcgacg gcttcgcggt tgagttcgct caatccgtac acggcttggg aaacgaggcc
     9061 gggggcggcg tgggtcatgc cgtaccagtg ttcccaggtc ggaatgtccc atatgcggaa
     9121 gtcggcggaa aggtcgatga cgcgcacgcc ctgttcaatc aggcgcggcg cgtctttcat
     9181 ggcgatgccg ttgggcgtgg cgaagaagac gatgtcgcat tgttccaaac ctgcctcgtc
     9241 gggcgtttgg aaggcgaggc cgtacacgcc gcgcaaactc ggaaagtaat cggcaactgc
     9301 ggttcccgct tcgctgcggc tggttacggc ggcgacttcg acatcgggat gggcggcaag
     9361 caggcgcagc agttccacgc cggtgtagcc cgtcgcgccg acaatgccga ctttgatttt
     9421 tttactcatg gtttttcctt tgtgtggttg gcgggtatgc cgtccgaacg ctgtccgacg
     9481 gggttttgat tggcggcgga aggacggtac acaaagcgtt tgaaaagcgt gtctaattta
     9541 atggcatccg cctctgtttt taaaacccag ccctgccgtc cggaataaac ataaccgtag
     9601 gcgcgaaagc cggcaggata tttcggtgcc tgccccaaac ccaaaccgta tgcggcgaaa
     9661 gcggtggtcg tggagaagga aagcaggagg aggacgaggg ttttcataag gttccaaacc
     9721 gaacggggcg atgaagcccg attatagcaa aaggtcgtct gaaaaccgtt ttcagacgac
     9781 cttttattta attacaataa aaatgttaac aacaaaaaac aaaccgcttt tttccgtttg
     9841 gagacaaatt tttaactaaa attgtcaaca attccttgac gcacacacaa aaaaaacttt
     9901 aatatttgta aatatatgta acattataaa ttttaaatat tttctgagta aggggaagta
     9961 atgagcatcc atctcgactt cggcattagt cctaaaacgt tccgacagac ttatctgtat
    10021 caaaagccca agctctttaa aggagcggtt cggaatctcg aatccgcatc ttggaaagat
    10081 atcaacgaga tataccaacg agcagaccca accgcaccgc tgtttcatct gcgtaaaaaa
    10141 ggcgcaatcg ttcctaaaga agaatacgtc gaaagtttcg acgatttggg caaaactcgc
    10201 taccgtttta ttaaatccgt tatctacgaa catatgaaga atggtgcgtc gttagtctat
    10261 aaccatatta acaacgagcc gttttcagac catatcgccc gtcaagtcgc ccgctttgcc
    10321 ggcgcacata ctattgttag tggatatctt gcttttggca gcgacgaatc ttataaaaac
    10381 cattgggacc cccgcgatgt gtatgccatc cagcttttcg gcaagaaacg ttggcaactt
    10441 actgcccctg atttccctat gccattgtat atgcaacaga ctaaagatac tgatatttcc
    10501 attcctgaac atatcgatat ggatattatc cttgaagcag gtgatgtcct ctacatccca
    10561 cgcggttggt ggcacagacc tatcccgctc ggctgtgaaa ccttccactt cgctgtcggt
    10621 accttcccac caaacggcta taattacctc gagtggctaa tgaagaaatt tcccaccata
    10681 gaaagtctgc gccacagttt ctcagactgg gagcaagata ggacgcgtat caacgatact
    10741 gccacacaaa ttgctgccat gattgccgac cccgtcaatt atgaagcctt cagtgaagac
    10801 tttctcggca aagaacgtac cgataccgct tttcatctcg aacagttcgc gaatcccaac
    10861 gctactccgc tttcagacga cgtcaggttg agattaaatg ccaataattt ggatacgttg
    10921 gaaaagggat atttgattgg gaatgggatg aagataagcg tagatgagtt ggggaaaaaa
    10981 gtgttagaac acatcggtaa gaatgaaccg ttattgttga aaaatctact ggttaacttc
    11041 aatcagggaa aatatgaaga agttaggaag ttgatttatc agttgataga gttagatttt
    11101 ctggaaattt tgtgagggat tctatgaaaa actggaagca atttacattt ttcgtaatat
    11161 tagtaatagc ttgttatcaa gtgctatatt ttttatcaga tatgtttctt ctcgactaca
    11221 ttaacaaata tagttggaat ttgaatttta ttcagggtac tttgaatttt ttttcaatat
    11281 atctgccata tgtttttgtt agccgaattt ttaggaatac caaccaagaa aaggagtata
    11341 aaaatgattg aacttcaact tcatgaattg aagctggttt cagggggagg tcctgtaaca
    11401 gacaatatag ctggaaatgt agctaatgct gccacaacca aaggaggtcc cacatggggg
    11461 gatttggttg caatacctgc tgccgcagcg gcagtttatt atctgccgaa aaatgcttac
    11521 ggtgctgctg gtgcaaatgg tgtatacaat gtgactcgta attgggtaaa tgatgccgtt
    11581 aatgcacctc cttataacgg aagaccaatc tttgagattg aacatggatt aactgctccc
    11641 gcaacaaaag cagataaatc aggaaacggc tacactgacg gtacagatta ctgctgatat
    11701 ttctcatcgt ccagacaatc tctaggggtc gtctgaaact ttcttaactt taattttatg
    11761 aatagaccca agcaaccctt cttccgtccc gaagtcgccg ttgcccgcca aaccagcctg
    11821 acgggtaaag tgattctgac acgaccgttg tcattttccc tatggacgac atttgcatcg
    11881 atatctgcgt tattgattat cctgtttttg atatttggta actatacgcg aaagacaaca
    11941 gtggagggac aaattttacc tgcatcgggc gtaatcaggg tgtatgcacc ggatacgggg
    12001 acaattacag cgaaattcgt ggaagatgga gaaaaggtta aggctggcga caagctattt
    12061 gcgctttcga cctcacgttt cggcgcagga ggtagcgtgc agcagcagtt gaaaacggag
    12121 gcagttttga agaaaacgtt ggcagaacag gaactgggtc gtctgaagct gatacacgag
    12181 aatgaaacgc gcagccttaa agcaactgtc gaacgtttgg aaaaccagaa actccatatt
    12241 ttgcaacaga tagacggtca gaaaaggcgc attagacttg cggaagatca gtggcaaaaa
    12301 cggcactggg cagacaggaa ttgtcgggat tgggcatggt atcctccgat ttggcgaaga
    12361 gcaacgaacc tgtttatctc gtgaaaataa aacccgacaa accaaccatc actgcatacg
    12421 gtgaggaaaa accgctgcaa atcggcatga cgttggaagc agacatccta cacgagaaac
    12481 ggcgattgta cgaatgggta ttggagctga tttatagtat gtcgggcaaa ctgtaagact
    12541 tgaggctgta aagataaaga agttgtctga aaccttttca gacgaccaga agacaa
//
LOCUS       DAXHTY010000058        12154 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXHTY010000058 DAXHTY010000000
VERSION     DAXHTY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.058"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(64..486)
                     /locus_tag="ACFNMK_001361"
     CDS             complement(64..486)
                     /locus_tag="ACFNMK_001361"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC6364623.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(497..1894)
                     /gene="atpD"
                     /locus_tag="ACFNMK_001362"
     CDS             complement(497..1894)
                     /gene="atpD"
                     /locus_tag="ACFNMK_001362"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC6364624.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGRIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(1932..2807)
                     /gene="atpG"
                     /locus_tag="ACFNMK_001363"
     CDS             complement(1932..2807)
                     /gene="atpG"
                     /locus_tag="ACFNMK_001363"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC6364625.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(2832..4379)
                     /gene="atpA"
                     /locus_tag="ACFNMK_001364"
     CDS             complement(2832..4379)
                     /gene="atpA"
                     /locus_tag="ACFNMK_001364"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC6364626.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(4390..4923)
                     /locus_tag="ACFNMK_001365"
     CDS             complement(4390..4923)
                     /locus_tag="ACFNMK_001365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC6364627.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(4928..5398)
                     /locus_tag="ACFNMK_001366"
     CDS             complement(4928..5398)
                     /locus_tag="ACFNMK_001366"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC6364628.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(5469..5705)
                     /gene="atpE"
                     /locus_tag="ACFNMK_001367"
     CDS             complement(5469..5705)
                     /gene="atpE"
                     /locus_tag="ACFNMK_001367"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC6364629.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(5762..6628)
                     /gene="atpB"
                     /locus_tag="ACFNMK_001368"
     CDS             complement(5762..6628)
                     /gene="atpB"
                     /locus_tag="ACFNMK_001368"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC6364630.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(6618..6971)
                     /locus_tag="ACFNMK_001369"
     CDS             complement(6618..6971)
                     /locus_tag="ACFNMK_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC6364631.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            complement(7339..7677)
                     /locus_tag="ACFNMK_001370"
     CDS             complement(7339..7677)
                     /locus_tag="ACFNMK_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364632.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            complement(7812..8672)
                     /locus_tag="ACFNMK_001371"
     CDS             complement(7812..8672)
                     /locus_tag="ACFNMK_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC6364633.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            8830..9399
                     /locus_tag="ACFNMK_001372"
     CDS             8830..9399
                     /locus_tag="ACFNMK_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC6364634.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            complement(9461..10324)
                     /locus_tag="ACFNMK_001373"
     CDS             complement(9461..10324)
                     /locus_tag="ACFNMK_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6364635.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(10484..11170)
                     /locus_tag="ACFNMK_001374"
     CDS             complement(10484..11170)
                     /locus_tag="ACFNMK_001374"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC6364636.1"
                     /translation="MAGLTFEQTVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NTLARKLDKR"
     gene            complement(11173..11910)
                     /locus_tag="ACFNMK_001375"
     CDS             complement(11173..11910)
                     /locus_tag="ACFNMK_001375"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC6364637.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVKECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
BASE COUNT         2922 a         3144 c         3114 g         2974 t
ORIGIN      
        1 tttcaaaact tggaaaaata aaaagcacga ccaaacggtc gtgcttttcc aaaccaaaca
       61 agtttatttc ttgtgcgaac ggatatagtc caaagttttg agctgtgcaa tcgcagcagc
      121 caatgcttta tgcgcctccg ccaaagcctt atcgtcttta gcttgggaaa tgcccgcttc
      181 tgcggctttt ttcgcctctt ccgcacgtgc ctgatccatc tccgcactgc ggacggcaac
      241 atccgccaag acagttactt tatcaggctg tacttccaaa acaccgccgg aaacagcaac
      301 caaaacctct ttatcctcgc ccggaacggt caaacgcaaa gcccccggcc gcaccaaact
      361 cataatcggc tcgtgtcgcg ggtaaatacc aagctcacct tgaacggtcg gaaccactac
      421 aaagctggct tcgcccgaat agatgttttg ctcactactg accacctcaa cttgcatgat
      481 gctcatgccg acctcctcag tttaaggttt tcgctttctc aaccgcttct tcgatgctgc
      541 cgaccatgta gaatgcctgt tcgggcagat gatcgtattc gccgttcaag atggctttga
      601 agccggcaat ggtatcgcgc agggcgacat atttgcccgg agaacctgta aacacttcgg
      661 caacgtggaa cggttgggac aggaagcgtt ggattttacg cgcacgcatt acggtcagtt
      721 tgtcttcgtc agacaattcg tccataccca agatggcgat gatgtcgcgc aattctttgt
      781 atttctgcag ggtggactgt acgccgcgcg ccacgtcgta gtgctcttga cccaagacca
      841 tcggatccag ttggcgcgaa gtggaatcaa gcggatcgac tgccgggtaa atacccaaag
      901 aggcaatatc gcggctcaat acgacggttg cgtccaagtg ggcgaacgtt gttgccggag
      961 acgggtcggt caagtcgtcc gcaggtacat atacggcttg gatagaagta atagaaccgg
     1021 tttgggtaga ggtaatacgc tcctgcaaac gacccatttc ttctgccaat gtcggttggt
     1081 agcccactgc agacggcata cggcccaaca atgcggatac ttcggtacca gccagagtgt
     1141 aacggtagat gttgtccacg aagaacaata cgtcgcggcc tttgccgttt tcgtcttttt
     1201 cgtcacggaa gtattccgcc atggtcaaac cggtcaatgc gacgcgcaaa cggttgcccg
     1261 gaggttcgtt catctgaccg taaaccattg ccactttatc caatacgttg gaatctttca
     1321 tctcgtggta gaagtcgtta ccttcgcggg tacgctcacc cacgcctgcg aacacggaca
     1381 agccgctgtg cgctttggcg atgttgttga tcaattccat catgttcacg gttttaccca
     1441 caccggcacc gccgaacaga cctactttac cgcctttggc aaacggacac agcaagtcaa
     1501 tcactttaat gcccgtttcg agcaattcgg ttgtggaaga cagttcgtca aacttaggag
     1561 cggcttggtg gatggcacgg ctcttgtcgg tatcgattcg acctgcctcg tcaacaggcg
     1621 ttcccaatac atcgacaatg cgtcccaacg tacctttacc taccggcaca gtaatgggcg
     1681 caccggtatt gctcacagtc atgccgcgtt tcaaaccgtc cgagctgccc atcgcaatgg
     1741 tacggactac gccgtcgccc aaaagctgtt ggacttccaa agtcagaccg ttttcgtcta
     1801 atttcaaagc gtcgtaaacg cgcggaatca tgtcgcgtgg aaattccacg tcaacaaccg
     1861 caccgataat ttgtacgatt ttgccttggc tcattatcgt atcctaattt ccgtacagga
     1921 ttcagacggc atcagacagc cgccgcacct gctacaattt ctgacaattc cgtggtaatc
     1981 gcagcttgac gcgatttgtt atataccaaa cgcaactctt tgatggcatt gcctgcattg
     2041 tctgttgcag ctttcatggc aaccatgcgg gctgcctgtt cggatgccat attgtcgctc
     2101 aacgcctgat aaaccacaga ctctaaatag cggcgaacca gatattccaa cactgcaagt
     2161 gcagtcggtt cgtagcggta ttcccagctg aacggtgatt tgggagctga atcgccaatc
     2221 acgttctcac cgataggcag caatacttcc attctcggtt cttgacgcat ggtattgaca
     2281 aaacccgaat acaccagatg gattctgtca atttcatgtt tctcataccg ttggaagagt
     2341 tctgtcaaag gtccgagcag catttccatt tttggggtat cgcccaaatt tacggcactg
     2401 gcaaccacat tcagaccaat gctctgacac gccatcagac ctttactgcc aaggcatacg
     2461 atttcctctt caataccttg attccgatac tcttgaactt gtgccaaaaa ctttttcagc
     2521 acgttggcgt tcaaaccgcc acacaaaccc ttatcagacg taatcaaaat aaaaccgaca
     2581 cgtctgattt cccgatgaga ttccagtaac ggaataccat gatcggtatt ggtttgcgca
     2641 agatggctca tcaccatacg cactttttcg gcatacggac gcgccaaacg catccgttcc
     2701 tgagtcttcc gcattttaga ggttgacacc atctgcatcg ctttagtgat cttttgggta
     2761 ttctgaacac tgcggatttt ggtgagaatc tcttttccta ctgccatttc agactccttt
     2821 cacttcaagc cttatgcctg ataggcgtaa gaagatttga aggatttcat ggctgcttca
     2881 agcgttttct cgctctcgtc ggacattgca cctgaagcat tgacggcttc caaaacttcc
     2941 ggatgttggg tacggacaaa gctcaaaaat tcagattcaa aagccagagc tttggcaacc
     3001 ggaacatcag aatacgaacc gttgttgatt gcccaaaggg tcaaagccat ttcagccgta
     3061 ttcaacgtac tgaactgttt ctgtttcatc agttcggtta cgacttcgcc atgctccaat
     3121 tgtttgcgcg tagcttcatc caaatcggat gcaaattgcg agaacgccgc caattcacga
     3181 tattgtgcca acgccaaacg gataccgcca cccagctttt taatcacttt ggtttgtgca
     3241 gcaccgccta cgcgggatac ggaaataccg gcattgattg caggacggat accggcgttg
     3301 aagaggtcgg tttccaagaa aatctgaccg tcggtaatcg aaatgacgtt agtcggaacg
     3361 aaagcagata cgtcgcccgc ttgggtttcg ataatcggca aagcagtcag agaaccggtt
     3421 ttgcctttta cttcgccgtt ggtcaatttc tccacttcgt gttcattgac acgtgctgca
     3481 cgttccaaca gacgggagtg caggtagaac acatcgccgg gataggcttc gcggccgggc
     3541 ggacggcgca aaagcaggga aatttgacgg taagccacag cctgtttgga caaatcgtca
     3601 taaacaatca aggcatcttc gccgcggtcg cggaagaatt cacccatcgt acaaccggag
     3661 taaggtgcga tatattgcaa tgccgccgct tcagatgcag ttgcagcaac cacaatcgtg
     3721 tgttccatcg cgccatgctc ttccaatttg cggaccacgt tggcaataga agatgctttt
     3781 tgaccgatag cgacatagat acagataaca cccgtacctt tttggttgac gatggcatcc
     3841 agcgctacgg ccgttttacc ggtttgacgg tcgccaataa tcaactcacg ctgaccgcga
     3901 ccgacaggaa ccatagagtc aatcgccttc agaccggttt gcatcggctg gtcaaccgat
     3961 ttgcgcgcaa tcacgcccgg tgcgattttt tcgatagggg cggtcaaagt tgtattaatc
     4021 gggcctttgc cgtcgatagg ccgacccaat gcatcaacga cgcgtccgac cagttcgcgt
     4081 ccgaccggca cttccaagat acgaccggta caggtaaccg tgtcgccttc tttaatatgt
     4141 tcgtactcgc ccaacactac ggcgccgacg gagtcgcgct ccaggttcat cgccaagccg
     4201 aaagtgttac ccgggaattc gagcatctca ccttgcattg catctgacaa accatggatg
     4261 cgaacgatac cgtcagttac cgaaatcacc gtaccacggg tacgcacttc ggcatttaca
     4321 gacagatttt cgatcttggc tttaatcaaa tcgctaattt cagcaggatt aagctgcatg
     4381 aaaactctcc taattcgtca tagtcgtgta caaggcactc aatttgcctt gtacagacaa
     4441 atccaaaacc tgatcaccca cttcaacttt tatgccgcca atcagctccg gttcgatttc
     4501 gacagagatt ttcagctcgc tgtcgaaacg cttattcagc atttgcgcca actcgccgac
     4561 ctgtttgtcg gtcaacggat aggcactgta aatgacggca gatttgatat ggttgaatga
     4621 taaggtcaag tcttgatatt gagcatatac ttccggcaat atcgacaaac gtttctgccc
     4681 ggccaagacg ataacaaagt ttttcaactc cttgtctttc aaaccgacca aatcgatgag
     4741 gatatctgct ttttctgaag cattcgtttc aggacggtca atcaatgaag ccaccttccc
     4801 ttcctgaaca accgccgcaa gtttttccag tccgcccaac caagactcaa tttggctttt
     4861 ttcctgagcc agaccgaaca atgcctttgc ataaggtctg gcaatcgttg cgaactctgc
     4921 cataagatta cagctcctgt ttcagggtat cgagcagttt tgcgtgtttg gaagcatcga
     4981 cttcgctgcg caaaatagat tcggcacctt tgacagccaa cacggcaacc tgctcgcgca
     5041 gggattcgcg tgcgcggaac aattcctgct ccacatcggc ctttgcctga gctgcaatgc
     5101 gcgccgcctc ggaagaagcc tgttctttgg cttcttcgac aattttggcg gcacgttttt
     5161 cggcgttggc aaccatttcg gaaacctgat tacgcccttc tgccaagagt tctgcaacct
     5221 ttttttcagc ctgctcgaaa tcgcttttac cacgctcggc ggcagccaag ccttcggcga
     5281 cttttgcggc acgctcatcc aaagcttttg caatcggcgg ccacacgaat ttcatggtaa
     5341 accataccaa accgaaaaag acgatgattt gagcgaataa tgttgcattg atattcacgt
     5401 tacttaacct tcgtactggg gttaatcaaa caggctgcgc ctgtacggaa cggacgaatc
     5461 cgtcctgatt atgcacctgc aaacgggtta acgaaggcga acagcagtgc aatggcgaca
     5521 ccaatcaaga atgcggcatc aatcaaaccg gcaatcagga acagtttggt ttgcagcgga
     5581 ccgatcagtt cgggctgacg ggcagaagac tccaaatatt tagaaccgac cattgcgata
     5641 ccgatagagg cacccaatgc acccaatgca acgatcaaac cacatgcgat agcaatcaaa
     5701 cccattttaa actccttaaa gaaacaaagg ttaaactaca aaaacaaact acttaggaaa
     5761 atcagtgcgc atcatgtgcc tgtccgatat agacgaacgc caacgccatg aaaataaacg
     5821 cctgcagggt aatcaccaaa atatggaaaa tcgcccatgc caaaccggca ataatgtgga
     5881 atacaaacag aatcggatcc atgacttcga cgctgccgga agccgcccaa gcaccgccaa
     5941 gcaaggctat cagcaagaat accaactcgc ccgcatacat attgccgaac aaccgcatac
     6001 cgtgggatac ggttttagaa agaaactcga ccaaattcaa cagaaagttc gcaggtgcga
     6061 gttttgcacc gaacggcgcg ctgaacaact cgtgaaacca gccacccaat cctttgattt
     6121 tgatgttgta atagatacaa atcagcaaca cgccgacagc gagtgccaaa gtggtgttca
     6181 aatcggcagt cggtacgacg cgcagcaggg cgtgatggtt gccggtaatg ccctgccata
     6241 ccatcggcag caaatcgacc ggcagcatat ccatcgcgtt catcagaaaa atccagacaa
     6301 acagcgtcag acccaacggc gcgacggctt ttctagactt ttcgttgtga atgatgctct
     6361 tacacatatc gtccacaaac tcaaacaaga tttccacggc agcctggaaa cgtccgggaa
     6421 cgcctgccgt tgcttttttc gcaccgcgcc acaacagaaa gctgccgatt acgcccaaca
     6481 ggacggcaaa aaagacggca tcaaggttaa taaacgaaaa atcagcaatg tttttcagtc
     6541 cctgaccctg agtaacatcc gacaaactgg tcaagctctg caagtggtgc ttgatgtagt
     6601 cggcagcggt aatggtttca cctgccataa tctttcactc tcaacaatac taaaaaaacc
     6661 aaatggctga caccgagcag ccccatcaga aacggggcga acaccagcga ttgatgccat
     6721 attgcaaata cggcgagcat ggacaacagc gacagcacta cttttaaaat ctctccgaag
     6781 acgaacatcc tgctttgcag gaaagggttt cccctgaaaa gttttaaaag taaaactgca
     6841 acaaacgtgg gaagcaggta ggacaaaccg ccaccgaccg ccgaaaggaa tccggcaaaa
     6901 ccccatacag caaaggcaac tgcggcgcat atggacaata cggcggattg taggatgata
     6961 atctgcttca taaagggaat gtttccgcct cggatttggg gcgcggctaa tataatttag
     7021 aagccttatt acgtcaagcg acagttaatc tttgtgaaac aacgtatccc aatccgccgc
     7081 gctcgccgcc tgaataacgg cgacaggtgt cattctaaca cacattacat ataattacag
     7141 gatattaagg agtttgtccg caatttcttt acacttttaa tgttcttacg tgatttgttt
     7201 gctttacgtg gaaataataa aaaatcaacg cgaaattgta gcagtttatc ggtcggattg
     7261 tcggcagttt ggggaatttg ctcaataaat aaaaggtcgt ctgaaaatat tttcagacga
     7321 ccttttccga ataaaggatt agcaacggcc tgccgcttta agcaaagcat tgcattgact
     7381 tttgcctttg tgcgttccgc ctcccaaaca aattgcatcg gaagtggtaa cgccgattgt
     7441 gctgattaca ctggtaacat agcattggct cacgcgctta cccacagttg cggtaaagtt
     7501 gatgcgtatg ccttcattgc tgcggttgct gatttttacg gcatttgggc tgacgcccaa
     7561 ggcaaacgcg gcacgttcct gaagtttctg gtcggaaacg gttacattat tgattgagcc
     7621 gcaacctgct aatgccaacg caacgaacgc agccgaaacg atgatgcgtg tgttcataat
     7681 ttcctcgaaa attaaaaatg aaaacaggaa aacgattctt acgtgaagca gaaaaatgtc
     7741 aatagaatta tatttcccac ttaaaatctg gaaagctgtt ctctatattt cagacggtat
     7801 atcccgcaaa attaaggtcg gtaatctatg cccaactgct tcaagatatg gtcgaaggtt
     7861 tcgggcgtgt cgaaatgcag gatgattctg ccttttttat ggttggcggt tttgatttcg
     7921 gcgttgacac ccagtttttc agtcagcaaa tcattcaggc ggccgatgtc ggcggcggca
     7981 gtttttttgg gctcgggacg tttgttttga agggcggctt ggctgcggcg ttcgacttcg
     8041 cgcaccgacc agccgttttt gacagccttt tgcgccaatt cgagctgttc gacgacgggc
     8101 agggtcagca gtgcgcgggc gtgccccatt tcgaggcggc gttggtaaag catttcctgc
     8161 acgggttcgg gcaggcttaa aaggcgcagg ctgttggaaa tcgcgcttcg gcttttaccg
     8221 acggcttggg cgatggtttc gtgggtcagc ccgaactcgt cggcaaggcg tttcaagcct
     8281 tgtgcttctt cgatggggtt gaggttttcg cgctggaggt tttcgatcaa acccattgcc
     8341 aacgcggttt cgtcgctgat ggttttgata acggcgggga tttcggtcag gccggcaatc
     8401 tgtgcggcac gccaacgccg ctcccccgca atcagttcgt atcgggacag tccgtgttcg
     8461 cgcacgatga cgggctgtat cacgccttgc gccttaatcg aatctgccag ctcctgcaag
     8521 gcttcgtcat cgatttgaac acgcgcctga tagcggccgg gccggatatc tttaaccgca
     8581 accgtggtca atcggtcgcc gctgctgttg tctgcgccgt tggcgagcag cgaatccaaa
     8641 ccgcgcccca atccgccttt tacttttgcc ataccgccct cccgtgccta ttcagatagg
     8701 atgttaaatc ggatatttta tcggatattg ggtgttgccg acaatttgta tccgtattta
     8761 tcggatttct gttttttcac tataatagcc ggtttgccgt tgcaggcggt tttatgggaa
     8821 aggcggatga tggtacggcg tttgataatc ggcatcagtg gggcgagcgg tttccaatac
     8881 ggcgtgaagg ctttggaact tttacgcgcg caagatatcg aaacgcacct tgtggtatcg
     8941 aaaggtgcgg agatggcgcg cgcttcggaa acggcttatg cgagagacga ggtatatgcc
     9001 ctcgcggact tcgtgcatcc gatcggcaat atcggggcgt gcattgccag cggtacgttt
     9061 aaaacggacg ggatgctggt cgccccctgt tcgatgcgga cgcttgcctc ggtcgcgcac
     9121 ggcttcggcg acaacctctt gacgcgtgcg gcggatgtgg ttttgaagga aaggcggcgg
     9181 ctggtgctga tggtgcgcga aacgccgctg aaccttgccc atttggacaa tatgaagcgg
     9241 gtaacggaaa tgggcggcgt ggtgtttccc cctgttcctg cgatgtaccg caaaccgcag
     9301 acggcggacg acatagtggc gcacagtgtt gcacacgctt tgtcgctgtt cggaatcgat
     9361 acgccggatt cggcggaatg gcagggaatg gcggattaaa ggacaaaaat gccgtctgaa
     9421 cacggatacg gttcagacgg catcatttta tacgactgcc ttatttggct gcgccttcat
     9481 tccatgcggc aggggatttg tagccctcga agcgtttgtg cgcgtaggct ttgaacgcgt
     9541 cggagttata ggcctcggtt acgtctttaa gccattggct gtctttgtcg gcggttttga
     9601 cggcagacca gttgacatag gcaaagctcg gttcttggaa cagggcttcg gtcagcttca
     9661 tgccgctgct tatggcgtag ttgccgttga cgacggcaaa atccacgtcg gcgcggctac
     9721 gcggcagttg cgcggcttca agctcgacga ttttgatgtt tttcaggttc tcggcgatgt
     9781 ccgctttgga tgcggtcaac ggattgatgc cgtctttgag tttgatccaa cccagttcgt
     9841 cgagcatcac caagacgcgg gcgaagttgg acgggtcgtt gggcgcggat acggtgctgc
     9901 cgtctttgac ttcttccagc gatttcagct tgcccgggta cagtcccaaa ggcgcggtcg
     9961 gcacttggaa gacttcggtg atgtccagat tgtgttcttt tttgaagtcg tcaagatagg
    10021 gtttgtgttg gaagacgttg atgtccaact cgccctcagc caatgccaga ttcgggcgta
    10081 catagtcggt aaactcgacc agtttgacgg tgtagccttt tttctccagc tcgggttgga
    10141 tttgttcttt gaccatatcg ccgaagtcgc cgacggtcgt gccgaagacg atttcttttt
    10201 tcgccgcgcc gttgtcggcg gcggcagaag cggatgcggc gggcgcgctg tctttttgac
    10261 cgccgcaggc ggcgaggatg agcgcgagtg cggcggcgga aagggttttg aagaaggttt
    10321 tcatattttc tcctgatgtt gtggcggttt caaacaaaaa tgacgggcag ggagtcctgc
    10381 cgtccggatt cggcgttcag acggcatttg ccgcgaacag gggaatttta tagcattttt
    10441 cggatagcgg tgggggtttt ggcgttcaga cggcattttg ggttcaacgt ttgtcgagtt
    10501 tccgcgccaa cgtgttgccg gtgctttgaa tcaggataac gagcagcacg aggagggcga
    10561 cgatgaagac gatgacttcg gtttggtagc ggtagtagcc gtagcggatg gcgaggtcgc
    10621 ccaagccgcc gccgcctatc atccctgccg ccgcgctgta tgacaaaagc ccgatggcaa
    10681 gcacggtaat gctggaaacc atgcccgcgc gcgcttcgtt caagaggact ttgcagacga
    10741 tggcaatcgg aggcgcaccc atcgcggcgg cggcttcaat cacgcctttg gggacttcgc
    10801 gcaggttttg ttccaccagt cgggcaaaat aaaacaatcc cgacacgctc aacaccagcg
    10861 aggcggcaac cgggccgatg gtgctgccga cgatggcgcg tgtggcgggt atcatcgcaa
    10921 tcatcaaaat gacgaagggg aaggcgcgca tgaggttgac gaggttgtcg agcaggaagt
    10981 tcaccggctt gttgtaatgc agttggcggc tggaagttac gaagagcaac acgcccagca
    11041 acgtgccgaa aacgacggcg aatgtggtgg acaaaccgac cattacgaag gtttcgccca
    11101 atgcgcggac gatttcgcct ttcataccga tgatgttgga aacggtttgt tcaaatgtta
    11161 agcctgccat attcagtcct cccgaatcag ttcgcgcccg atgtcggatc gggcgtggat
    11221 ttggttgccg cgtacttcga cgatttcgac gactttgcct ttatccaaca gggcggcgcg
    11281 gtcgcacagt cgtctgataa cgctcatttc gtgggttacg atgacgatgg ttacgttgaa
    11341 tcgtttgttg atgtcttcca aacattccaa gacgctgcgc gtggtggcgg ggtcgagggc
    11401 ggaagtgggt tcgtcggcga ggatgacttg gggtttgggc gcgagtgcgc gggcgatgcc
    11461 gacacgttgt ttctgcccgc cggaaagctg ggcgggatag tggccggcgc gttcggtcaa
    11521 gccgacgatt tcaaggcatt ctttaacgcg cgctttgatt ttttcagacg gccatccggc
    11581 gatttccaaa ggaaaggcga cattgccggc gacggtgcgg ttgttcaaca ggttgaactg
    11641 ctgaaacacc atgccgatat tctgccgcgc ctgacgcaat gcggcggcat caagcgcggt
    11701 cagctcttgt ccgcagacgt tgaccttgcc cgtgtcgggg cgttccaaca ggttaatcag
    11761 ccgcaacagg gtggatttgc ccgcgccgga atagcccatc agcccgaaga tttcgccgtc
    11821 gcggatttca aggctggtcg gctcgacggc ggcaaaacgg gtcttgtcgc gcgtttggta
    11881 atgcttggaa accttgtcca aaataatcat tgtctttccc atacaacaaa gcccgatgca
    11941 ggacacaacg ggcgcggaag ataaagctga aattgtcgga acgctttagc tgttatgccc
    12001 gcaagctgtg tcaaatcggc aggttaattt tcgtaggata ttatcgggaa agcatttttt
    12061 tgtcaataaa gcaggaagcg ggcaaccatt tcggacaatg ccgtctgaaa cgggcaaagg
    12121 cagcggttcg caccaaaacg gcaaataatt gaaa
//
LOCUS       DAXHTY010000059        11830 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXHTY010000059 DAXHTY010000000
VERSION     DAXHTY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11830
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.059"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..221
                     /locus_tag="ACFNMK_001376"
     CDS             <1..221
                     /locus_tag="ACFNMK_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364638.1"
                     /translation="YADAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATLLALDWQ
                     GNKPLGAEELADLKPLYKDLKNNIGNIV"
     gene            546..977
                     /locus_tag="ACFNMK_001377"
     CDS             546..977
                     /locus_tag="ACFNMK_001377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364639.1"
                     /translation="MNMYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWG
                     DGYDVYRQLRWQQISEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAME
                     LKAFPCLSSLFLTKWHKNLYDSGLTKTSTALPRLSSKRTIL"
     gene            complement(1119..3419)
                     /gene="recQ"
                     /locus_tag="ACFNMK_001378"
     CDS             complement(1119..3419)
                     /gene="recQ"
                     /locus_tag="ACFNMK_001378"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC6364640.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSSTSADEAREIAD
                     KLAQCRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMAGQSGIVYCLSRKKVEDVARFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
     gene            complement(3484..4266)
                     /gene="trpC"
                     /locus_tag="ACFNMK_001379"
     CDS             complement(3484..4266)
                     /gene="trpC"
                     /locus_tag="ACFNMK_001379"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC6364641.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNTEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPILRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLHALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIEAEVGKLF"
     gene            complement(4317..5273)
                     /locus_tag="ACFNMK_001380"
     CDS             complement(4317..5273)
                     /locus_tag="ACFNMK_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HFC6364642.1"
                     /translation="MILTPPDTPFFLRNGNADTLAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSGSHYAVELMLAVRDRGWNGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRRTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNSR"
     gene            complement(5406..6944)
                     /gene="murJ"
                     /locus_tag="ACFNMK_001381"
     CDS             complement(5406..6944)
                     /gene="murJ"
                     /locus_tag="ACFNMK_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC6364643.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPSKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVEN"
     gene            7002..7109
                     /locus_tag="ACFNMK_001382"
                     /pseudo
     CDS             7002..7109
                     /locus_tag="ACFNMK_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7403..8101
                     /locus_tag="ACFNMK_001383"
     CDS             7403..8101
                     /locus_tag="ACFNMK_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6364644.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAAESKDVANSHIFDAMVNQKIKLQNPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMHTID
                     LLADKVREEQKAAQ"
     gene            complement(8158..9162)
                     /gene="amgK"
                     /locus_tag="ACFNMK_001384"
     CDS             complement(8158..9162)
                     /gene="amgK"
                     /locus_tag="ACFNMK_001384"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC6364645.1"
                     /translation="MQRQIELKNWLQTIYSGRSFELAFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQILHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYHPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            9232..11640
                     /locus_tag="ACFNMK_001385"
     CDS             9232..11640
                     /locus_tag="ACFNMK_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC6364646.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGKPEKTESAVQGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
BASE COUNT         2761 a         3253 c         3282 g         2534 t
ORIGIN      
        1 tatatgctga tgccggagac gtatattgcg tctataacat cagactgaag cagtacactg
       61 cctgccaggt tacccgagtt gaagaacacg gtggcaaaaa aacacatgcg accctgctgg
      121 ctttggactg gcagggcaac aaaccgcttg gggcggagga gctggcggat ttgaaaccgc
      181 tttacaaaga cttaaagaat aatattggaa atattgtatg aacaaaaaat taaactatat
      241 ttttatgttg gactgtttag ggttggtgat attgtttact tgtataatag ctacttttga
      301 aagagattat ggatttaaaa tttttactaa ttctaagagg cctgaatttt attattggat
      361 tggaatgttt tattatggaa ttatttcttg ctggtttgat tatcaattaa tttcaacaaa
      421 ggcgaattcg tataaaagaa aagttaaaca atataaaatt ttttcagtaa tattttcagt
      481 tttgatattt atttctacta tagtaaaact ttaaattttg gagcaaaaat ttatgagcga
      541 ttcaattgaa tatgtattgg gaacgcggtc tgcacatgta taaggcaagt gccgtcgtgc
      601 cgacgggata tgtacgggtt gggaataccg cgccgctggt cggcgaagac acgcaacggt
      661 atgcctcttt ttggggcgac ggctacgacg tgtaccgtca gttgagatgg cagcagatat
      721 ccgaaaaaca gagaaaggca ttcaaaaaag ccgctaaaag caaaaagacc gtgatgtttg
      781 ccggacggga atacggcata tccaaacaga atttgagcga tgtttgggat gattttgaag
      841 acgcgatgga actgaaggcg tttccctgcc tgtcttcgct gtttctgacc aaatggcata
      901 aaaatctata tgatagtgga ttaacaaaaa ccagtacggc gttgcctcgc cttagctcaa
      961 agagaacgat tctctaaggt gctgaagcac caagtgaatc ggttccgtac tatccgtact
     1021 gtctgcggct cgccgccttg tcctgatttt tgttaatcca ctataaaaac aggaattttt
     1081 aaatagaggc aatgccgtct gaaacttggt aacgggcttc agacggcatt tcgttccaat
     1141 accgccaaca ccgccgcacc gtaacgtgcg gctttttctt cgcctacgcc gtatacggcg
     1201 gcaagctccg ccaaaccttc cggctgtttg gcggcaatgg cgcgtagggc ggctttgctg
     1261 agaatgcggt agggttcgga ctgttcgtgt tttgccgttt cgttgcacca ttggatcagg
     1321 gcgcgcatca ggcggcgttt gtgtttggcg gtttcatcga tgccgtctga aaacggacgg
     1381 cagacggcga ggatgtcccg tccgtatttg gcggcgcgta cgctgcccaa gccgtgtacg
     1441 ccttcgaggt cggtttcggt ttcgggcgta tcggcaagca tatcggcaag gctttcgtcg
     1501 gagaggacgg catgcagggc gcagttttcc gcccttgcct gttcataccg ccaggcctcg
     1561 agtttttgac gcagttgttg ttcgcgttcg gtttgcggac ggatgaccgc gtcgcggctg
     1621 aagccggcgg cgttgcggca gacttcgagt atgccgtgtc cgaaacgttc ggttttggct
     1681 tcgcccaagc cgtagatgtc gtgcagcccg ttgaggtttt gcggcatttt ttcgacaagg
     1741 tcgcgcaggg ttttgtcgcc gaaaatcata taggcgggga tgccttcggc ttctgcctgt
     1801 ttcatacgcc aaacgcgcaa tgcctgccac aggcgttctt cgcgttcggt acgcagccag
     1861 ttgtctttga gggtgcgggc ggcgggcttg tcgcgcttga gcggacgcag catcacttcg
     1921 gtttcgcctt tgaggacttt tttggcggct tcggtcagtt gcaatgcctg atatcgggta
     1981 atgttgacgg tgaggtagcc gaggctgata cactggcgga tgacgctgcg ccattctttg
     2041 tcggacaact ccgtaccgat gccgaaggtg gacagttgtt cgtgccggtt gccgcgtatc
     2101 caatcgtcgc ttttgccgcg caaaaggttg gtgatgtaac cggcggcaaa acgttgtccg
     2161 gcgcggtaca cgcagctgag taatttttgc accaacaccg tgccgtcaaa ccgtacgggc
     2221 ggatgcaggc agttgtcgca atggccgcag ggttcggatt cttcgccgaa atgtttgagc
     2281 agcagtacgc ggcggcaggc ggcggtttcg cagacggaaa gcatggcatc gagtttttgc
     2341 atttcgattt gcttttgcac ctcgtcgctg ttgccttcgg caatccgttc gcgcagcaac
     2401 acccaatcgt tcaaaccgta acacagccag ctcgcggcag gcagcccgtc ccggccggca
     2461 cgccccgatt cctgatagaa atgttcgaca ctctggggca tatcgagatg ggcgacaaag
     2521 cgcacgtcgg gtttgtctat gcccatgccg aacgccacgg tcgccaccac gataatattg
     2581 tcttcatgcg taaagcggcg ttggttttcc tcgcgcacgt ccatgctcaa accggcatga
     2641 tacggaatcg cgtttaatcc gttttcacgc aaaaaccgcg ccacatcttc cacctttttg
     2701 cggcttaaac aatacacaat gccgctttgt cccgccattt ctttgcggat aaattcaagc
     2761 agttgttttt tgccgttatt tttttcgata acctgataat aaatattcgg gcggtcaaag
     2821 ctggagacaa attcgggcgc atcgtccaag tgcagataat gcttgatgtc ggcgcgtgtg
     2881 gccgcatcgg cggtggcggt cagggcgatg cgcgggacgt tcggatagcg ttcggcaagc
     2941 atgccgagct gttggtattc ggggcggaaa tcgtgtcccc actggctgac gcagtgcgct
     3001 tcgtcaatgg cgaacaggct gacggtttgt tggtcgagaa aacgcaaaaa gcggtcggta
     3061 accaagcgtt ccggtgcgac ataaagcagt ttcagacggc attgggcaag cttgtcggca
     3121 atctcgcgcg cttcatctgc cgatgtgctg ctgttgactg ccgccgcttc aatcccggct
     3181 acatgcaggc tggccacttg gtcgttcatc agcgcaatca gcggcgatac gacaaccgcc
     3241 acgccttcgc gcatcagcgc gggaatctgg taacacaaag acttgccgcc gcccgtcggc
     3301 atcagcaccg tcaaactccc gccgcctgcc aaagtattga tgacagcctc ctgcctgccg
     3361 cgaaattcgg gataaccgaa tacttcgtgc aaaatctgtt tggcggtcgg tcggtgcatg
     3421 atggttccgt gctcggtaag gatgttgatc ggtcggcggc aatatgccgt ctgaaatcgg
     3481 gatttagaag agtttgccca cttctgcttc aatatcgtcg gcacgcatga acgtttcgcc
     3541 gatcaggaag gtgtgcacgc cgcgcgcccg cataaattcc acatccgcct tacccgtaat
     3601 gccgctttcg gtaacgacgg ttttgccttc cagcgcgtgc agcagcgaca gggtttggtc
     3661 gagggagact tcaaaagtcc tcaggttgcg gttgtttaca ccccacaacg gcgtggtcag
     3721 gttgcggcat ttttccaatt cgctttcatc gtgcagctcg agcaggacgg tcatacccaa
     3781 ttcgtgcgcc accgcttcaa agcgttccaa ttgttcctgt tccagtgcgg cggcaatcag
     3841 caggacagca tccgcccccc aggcgcgcgc ctgataaacc tggtattcgt cgatgatgaa
     3901 gtctttacgc aaaatgggca gagatacggc ttcgcgcgcc tgtttgaggt attcgggcga
     3961 accttggaaa tagggttcgt cggtcagtac ggacaaacac gccgctccgg cgttttcata
     4021 ggcgcgtgca atctcggccg gtcggaagtc cggacggatc aggcctttgc tcgggcttgc
     4081 ttttttgatt tcggcaatga cggcgggcag gttgaggcgg tgtttgccgc gtatcgaatc
     4141 gatgaagctg cggacgggcg cggcttctgc ggcaagtgcg cggatgtgtt cggtgttgac
     4201 ggcggctttt tgggcggcaa cttcctgtgc tttggtggca aggattttat tgaggatgtc
     4261 ggtcatgtcg ggttccgtat tcgtctgggg aaagggggaa tattagcatc aaaccgttaa
     4321 cgactgtttg tgcggaagct gtcgaaatag gacaggacgg tctgcggcaa ccattgcagg
     4381 tgcagcctgc cgccggtgct gccgacaaag ccgacatgac caccgtgtgc cggctggaac
     4441 agggtaacgg cttcggacac ttcgtccggt gtcggcaacg cttcgggcgg caggaagggg
     4501 tcgttgacgg cattgagcag gagcagcggt ttggcaacgt gtttgagcag cggtttgcag
     4561 gaagttcggc ggtagtagtc gtgccggtcg gcaaagccgt gcagcggtgc ggtgaaacgg
     4621 tcgtcaaact cgcccagtgt tttgcaccct gcggcaaatg ccgtctgaaa accttggagc
     4681 gaccgtgctt tgggtatcag ggtgcggagg aagtagcgcg tgtagagcag ccgcgtgatg
     4741 ccgctgtcga agcgtctgcc tgccgcctct gcatcgacgg gggcggagat gacggcggcg
     4801 gcttgcggca gcgcgttttc gccctgttcg cccaaatatt ttgccagcgc gttgccgccc
     4861 agcgatacgc cgacggcgta tatttcacgg taacgcgcgg cgagcgtgtc caaagtaaag
     4921 gcgatttcgg cggtatcgcc caagtggtag aacaccgggg cggtgttggc aatgccgccg
     4981 cagctgcgga aatggacgac tgcgccgttc caaccgcgat cgcgtaccgc gagcatcagt
     5041 tcgaccgcgt aatggctgcc actgcttcct tccaaaccgt gaaacagcac gaccagcggt
     5101 gcgtcgggcg aaatgccgtc tgaaaagtcg taggcgactt tggttttacc cgtgctgtct
     5161 ggaagcagct cgcggcggta tgcaggtgcg gggcgttgca ggaatttggc ggccagcgtg
     5221 tcggcattgc cgttgcggag gaaaaagggc gtgtccggcg gtgttaaaat cataaggtat
     5281 cggttttctt gttttcagac ggcattgatg atgcggcagc ccgtccggcc ggtgcggacg
     5341 cgggggatgc gcgcccgaat ataggcgcgg aaaaagcgtt ttccgaaaaa ggatatcggc
     5401 atcggtcagt tttccacgcg tttgaaatgg cgcggacgga agcccaaagc cgccagtgat
     5461 gcgaaataca gtccgccgcc gacggcaatc aggatgcaga gctgccccgc tttccgcatt
     5521 ccgccggcgc gtacccattc aaacggcagc cagatttggg cggcatacag gccgcctccc
     5581 atcacggcga gcgagagcag catttttgct aagaacgctg cccaaccctt gctaggttgg
     5641 taaataccgt gtctgcgcaa caggtaaaac aacaatccgg cattgataca cgcgcccaga
     5701 ccgatggcaa gcgaaagtcc gacgtgtttc agtgggccga taaaggcaag gttcatcaac
     5761 tgcgtgcaga tgagcgtgaa gatggcgatt ttgacgggcg ttttgatgtt ttgccgcgca
     5821 tagaagccgg gtgccaacac tttaatcatg attaagccga ttaaaccgaa agaataggca
     5881 atcagcgcgt gttgcgtcat ctgcgcgtca aacagcgtga attctcggta cataaacaag
     5941 gttgccacca gcgggaacga caacaccgcc agtccgaccg ccgccggcag cgtcagcagc
     6001 atgcacaggc gcaaacccca gtcgagcagg gcggaaaact gttccgtatc ttggtttgcc
     6061 gagtgtttgg acaaagtcgg cagcaaaatc gtaccgagtg ccgcccccag cacgccgccg
     6121 ggcagctcca tcatgcggtc ggcgtaatac atccatgaaa cgctgcccga ttgcagataa
     6181 gacgcgaaaa tcgtgttgat aaccaaagaa atctgcgcca cgctcacgcc caaaatcgca
     6241 ggtgccattt gtttcatgac gcggttgacc gccgcatctt tgaaattcag tttgggcagt
     6301 ttcaaaaaac ccagtttcgc caaccaaggg agctgaaagc cgagctgcaa aataccgccg
     6361 acaaaaaccg cccaagccag cgcggtaacg ggaggatcga aatacggcac gaaaaacagc
     6421 gcgaatacga taaacgacac gttcaggaac gtgggcgtaa acgccggaat gctgaattta
     6481 tgataggaat tgagtaccga gccgacaaaa gaggaaagtg aaatcaataa gatatagggg
     6541 aacgtaatcc gcagcaaatc gatggagagc tgaaatttgt cggcatcttt ggcaaaaccg
     6601 ggtgcggaaa cataaatcac ccaaggcgcg gcaagtatgc ccagcgcggt aacgatgacc
     6661 agcacaaacg acagcatccc cgccacatgg cggataaaag cctccgtcgc ctctttagaa
     6721 cgcgtttcct tatattccgc caaaatcggc acaaacgctt gggcaaacgc cccctccgca
     6781 aacacgcggc gaagcaggtt gggcagtttg aacgcgacaa aaaacgcatc cgtcgccatg
     6841 cccgcgccga atgcgcgcgc aatgaccgta tcgcgcacaa atcccaaaac gcgcgacacc
     6901 atcgtcaggc tgccgacttt tgccaaagct cccagcatat tcatcgtatt tccttttcgg
     6961 ttgaaacccc gccctttagg gcggcaggat cagactttga tagtggatta aatttaaatc
     7021 aggacaaggc gacgaagccg cagacagtac aaatagtacg gcaaggcgag gcaacgctgt
     7081 actggtttaa atttaatcca ctatatttga aaggggtgta acccttccga accagggcaa
     7141 cgcatagggc ggtgctttat gtgccgtcct gtgtgttgga acatcattag ttccccaaca
     7201 gccgtatccg tctggggcga cggcgcgtat tgtacgacag aaaccgcttc agacggcatc
     7261 gggtttgatg ccgtctgaag cggtttcccg aaacgaaaac gtccttttcc ggcggcaaac
     7321 tgtatcaaca cgcggaaatg caataaaata gccggattcc gattgatttc caacatctgt
     7381 ttccaacatc acggagagcc gtatgaaatc cagacacctc gccctcggcg ttgccgccct
     7441 gttcgccctt gccgcgtgcg acagcaaagt ccaaaccagc gtccccgccg acagcgcgcc
     7501 tgccgcttcg gcagccgccg ccccggcagg gctggtcgaa gggcaaaact ataccgtcct
     7561 tgccaacccg attccccaac agcaggcagg caaagtcgaa gtccttgagt ttttcggcta
     7621 tttctgtccg cactgcgccc acctcgaacc tgttttaagc aaacacgcca agtcttttaa
     7681 agacgatatg tacctgcgta ccgaacacgt cgtctggcag aaagaaatgc tgacgctggc
     7741 ccgccttgcc gccgccgtcg atatggctgc cgccgaaagc aaagatgtgg cgaacagcca
     7801 tattttcgat gcgatggtca accaaaaaat caagctgcaa aatccggaag tcctcaaaaa
     7861 atggctgggc gaacaaaccg cctttgacgg caaaaaagtc cttgccgcct acgagtcccc
     7921 cgaaagccag gcgcgcgccg acaaaatgca ggagctgacc gaaaccttcc aaatcgacgg
     7981 tacgcccacg gttatcgtcg gcggcaaata taaagtcgaa tttgccgact gggagtccgg
     8041 tatgcacacc atcgaccttt tggcggacaa agtacgcgaa gaacaaaaag ccgcgcagta
     8101 agcccgtttg gaaaatgccg tctgaaactt ggttttcaga cggcattttg attgggttta
     8161 aaacgtaaag cccgtttcca gttcttcatc gccgaccagt tcgaccaaga gcgcgtagag
     8221 cggggcgagt tcggcataac ggcgcgatac gcggcgcaga tagtttaaga aacgcgggat
     8281 ttccggatgg tatttgtctt tgccgtcgcg gtagtacagg cgtgcgaaga tgcctgcaac
     8341 cttcaagtgc cgctgcacgc ccatccattc gaaccagcgg taaaactcgt caaacgcttc
     8401 ggggacgggc aggccggcag cccgcgcctt ttcccagtag cggataacca agtccaagac
     8461 aaattcttct tcccactcga taaaggcatc gcgcaacagc gacaccaaat cgtaggaaat
     8521 cgggccgtaa agcgcgtctt ggaagtctaa aacgcccggc ctgccgcgcg tcagcatcag
     8581 gttgcggacg ataaagtcgc ggtgcacata gactttgggc tgcgccaaca ggggcggcag
     8641 cagtgtatcg acggtctgct gccaaagttg gcgttgtttg aatgttaatt cgcgccccaa
     8701 ttcttttgcg acaaaccatt ccgggaacag gttaatttcg cgcaacatcg tttcacggtc
     8761 atattcgggc aaaacccctt cacggcttgc cttctgcaat tcgaccagct cgccgatggc
     8821 ctccagcagc agcgctttgt gcgccgtttc gccctgttcc tgaagcatcg cggttaaaaa
     8881 cgtcgtattg cccaagtcgt tcaataccac aaaccccaaa tccgtgtccg cgtgcaatat
     8941 ctgcggcaca ttgaccatgt caaacagttt ctgcactttc aaataaggtg cgacactcat
     9001 cttgtcaggc ggcgcatcca tgcagacgac actgctgccg tctgaaaacg ttgcacggaa
     9061 atagcggcgg aaatcagcat ctgccgccgc aaaggccagt tcgaaactgc ggccggaata
     9121 gatggtctga agccaatttt tcagttcgat ttgtcgttgc ataacagtac taaagcattt
     9181 caggttacaa taaacgctat tctaactggc aaaccgacct gaggggcaat tttggctcgt
     9241 ttattttcac tcaaaccact ggtgctggca ttgggcttct gcttcggcac gcattgcgcc
     9301 gccgccgatg ccgttgcggc ggaggaaacg gacaatccga ccgccggagg aagcgttcgg
     9361 agcgtgtccg aacccataca gcctaccagc ctgagcctcg gttcgacctg cctgttttgc
     9421 agtaacgaaa gcggcaaacc cgaaaaaacc gaatctgccg tccaaggcag cggcgaagca
     9481 tccatccccg aagactatac gcgcattgtt gccgacaagg tggaaggaca gtcgcaggtg
     9541 caggtgcgtg ccgaaggcaa cgtcgtcgtc gaacgcaacc ggacgaccct caatgccgat
     9601 tgggcggatt acgaccagtc gggcgacacc gttaccgcag gcgaccggtt cgccctccaa
     9661 caggacggta cgctgattcg gggcgaaacc ctgacctaca atctcgagca gcagaccggg
     9721 gaagcgcaca acgtccgtat ggaaaccgaa cacggcggac ggcggctgca aagcgtcagc
     9781 cgcaccgccg aaatgttggg cgaagggcat tacaaactga cggaaaccca attcaacacc
     9841 tgttccgccg gcgatgccgg ctggtatgtc aaggccgctt ccgtcgaagc cgatcgggaa
     9901 aaaggcatag gcgttgccaa acacgccgcc ttcgtgttcg gcggcgttcc cattttctac
     9961 accccttggg cggacttccc gcttgacggc aaccgcaaaa gcggcctgct tgttccctca
    10021 ctgtccgccg gttcggacgg cgtttccctt tccgttccct attatttcaa ccttgccccc
    10081 aatctcgatg ccacgttcgc gcccagcgtg atcggcgaac gcggcgcggt ctttgacggg
    10141 caggtacgct acctgcggcc ggattatgcc ggccagtccg acctgacctg gctgccgcac
    10201 gacaagaaaa gcggcaggaa taaccgctat caggcgaaat ggcagcatcg gcacgacatt
    10261 tccgacacgc ttcaggcggg tgtcgatttc aaccaagtct ccgacagcgg ctactaccgc
    10321 gacttttacg gcaacaaaga aatcgccggc aacgtcaacc tcaaccgccg tgtatggctg
    10381 gattatggcg gcagggcggc gggcggcagc ctgaatgccg gcctttcggt tctgaaatac
    10441 cagacgctgg caaaccaaag cggctacaaa gacaaaccgt atgccctgat gccgcgcctt
    10501 tccgccgatt ggcgcaaaaa caccggcagg gcgcaaatcg gcgtgtccgc acaatttacc
    10561 cgcttcagcc acgacagccg ccaagacggc agccgcctcg tcgtctatcc cgacatcaaa
    10621 tgggatttca gcaacagctg gggttacgtc cgtcccaaac tcggactgca cgccacctat
    10681 tacagcctca accgcttcgg cagccaagaa gcccgacgcg tcagccgcac tctgcccatt
    10741 gtcaacatcg acagcggcgc aacttttgag cggaatacgc ggatgttcgg cggcggagtc
    10801 ctgcaaaccc tcgagccgcg cctgttctac aactatattc ctgccaaatc ccaaaacgac
    10861 ctgcccaatt tcgattcgtc ggaaagcagc ttcggctacg ggcagctctt tcgcgaaaac
    10921 ctctattacg gcaacgacag gattaacacc gcaaacagcc tttccgccgc cgtgcaaagc
    10981 cgtattttgg acggcgcgac gggggaagag cgtttccgcg ccggcatcgg tcagaaattc
    11041 tatttcaagg atgatgcggt gatgcttgac ggcagcgtcg gcaaaaaacc gcgcaaccgt
    11101 tccgactggg tggcatttgc ctccggcagc atcggcagcc gcttcatcct cgacagcagc
    11161 atccactaca accaaaacga caaacgcgcc gagaactacg ccgtcggtgc aagctaccgt
    11221 cccgcacagg gcaaagtgct gaacgcccgc tacaaatacg ggcgcaacga aaaaatctac
    11281 ctgaagtccg acggttccta tttttacgac aaactcagcc agctcgacct gtccgcacaa
    11341 tggccgctga cgcgcaacct gtcggccgtc gtccgttaca actacggttt tgaagccaaa
    11401 aaaccgatag aggtgctggc gggtgcggaa tacaaaagca gttgcggctg ctggggcgcg
    11461 ggcgtgtacg cccaacgcta cgttaccggc gaaaacacct acaaaaacgc tgtctttttc
    11521 tcacttcagt tgaaagacct cagcagtgtc ggcagaaacc ccgcagacag gatggatgtc
    11581 gccgttcccg gctatatccc cgctcactct ctttccgccg gacgcaacaa acgaccctga
    11641 ccgtcggaaa cccggcagga gcaccgttcc cgaacaagac ggcattccac cgacaacccc
    11701 aaacccgcca tcaaaggtag gattcaaacg ataaggaaag aatgatgaaa atcaaagccc
    11761 tgatgattgc cgccgcattg ctggcagcag ccgatgtcca cgccgcaccg caaaaggcaa
    11821 aaaccgcatc
//
LOCUS       DAXHTY010000060        11642 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXHTY010000060 DAXHTY010000000
VERSION     DAXHTY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11642
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.060"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..132
                     /locus_tag="ACFNMK_001386"
     CDS             <1..132
                     /locus_tag="ACFNMK_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6364647.1"
                     /translation="RVLRKYNGIDCKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            211..492
                     /locus_tag="ACFNMK_001387"
     CDS             211..492
                     /locus_tag="ACFNMK_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6364648.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(519..1739)
                     /locus_tag="ACFNMK_001388"
     CDS             complement(519..1739)
                     /locus_tag="ACFNMK_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC6364649.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKRQ"
     gene            complement(1754..2179)
                     /locus_tag="ACFNMK_001389"
     CDS             complement(1754..2179)
                     /locus_tag="ACFNMK_001389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364650.1"
                     /translation="MPSEHAAYADLMASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAIQAFVPDGKIGYNIGHQCPTY"
     gene            2284..2943
                     /gene="queC"
                     /locus_tag="ACFNMK_001390"
     CDS             2284..2943
                     /gene="queC"
                     /locus_tag="ACFNMK_001390"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC6364651.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2971..3489
                     /locus_tag="ACFNMK_001391"
     CDS             2971..3489
                     /locus_tag="ACFNMK_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC6364652.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3496..3918
                     /gene="queD"
                     /locus_tag="ACFNMK_001392"
     CDS             3496..3918
                     /gene="queD"
                     /locus_tag="ACFNMK_001392"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC6364653.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4187..4558
                     /locus_tag="ACFNMK_001393"
     CDS             4187..4558
                     /locus_tag="ACFNMK_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC6364654.1"
                     /translation="MKLLFIPLVLFVAAEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4555..5193
                     /locus_tag="ACFNMK_001394"
     CDS             4555..5193
                     /locus_tag="ACFNMK_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC6364655.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGIPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQNGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(5714..5790)
                     /locus_tag="ACFNMK_001395"
     tRNA            complement(5714..5790)
                     /locus_tag="ACFNMK_001395"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5754..5756),aa:Pro,seq:cgg)
     gene            complement(6015..7100)
                     /gene="nagZ"
                     /locus_tag="ACFNMK_001396"
     CDS             complement(6015..7100)
                     /gene="nagZ"
                     /locus_tag="ACFNMK_001396"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC6364656.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGVILFRRNFQ
                     NIAQLKELTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAARQIGWVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVIQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(7157..8677)
                     /locus_tag="ACFNMK_001397"
     CDS             complement(7157..8677)
                     /locus_tag="ACFNMK_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC6364657.1"
                     /translation="MQLIDYSHSFFSVMPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDGSWGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(8879..10378)
                     /locus_tag="ACFNMK_001398"
     CDS             complement(8879..10378)
                     /locus_tag="ACFNMK_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC6364658.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALITKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            complement(10516..11145)
                     /gene="nth"
                     /locus_tag="ACFNMK_001399"
     CDS             complement(10516..11145)
                     /gene="nth"
                     /locus_tag="ACFNMK_001399"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC6364659.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(11191..11598)
                     /locus_tag="ACFNMK_001400"
     CDS             complement(11191..11598)
                     /locus_tag="ACFNMK_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC6364660.1"
                     /translation="MMPAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGALLC
                     AGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLAAG
                     WLRLNPWTSGLMLVVGKMARYAFILWGMQYYAA"
BASE COUNT         2778 a         3097 c         3194 g         2573 t
ORIGIN      
        1 cgtgtcttgc gaaaatacaa cggaatcgat tgcaaatctt tcccgctgtt cttgaaagaa
       61 tgcgaatttc gatttaactt cggcacaccg tctcaacagc ttaaaatcct gcgggattgg
      121 tgtggaattt agggctaatc tagtacagcc ccttgttttt tcgatacgga accggataga
      181 ggaaaaatcg aacattgcgc ctgccttgct atgattcacg aagaaatctc cgccatgcct
      241 atgggctatg aaaccttgat cggcgatatg ggcagcgcac tgtcaggcgg acaaaaacaa
      301 cgcatcgtat tggcgcgggc cttatattgc gaaccgaaaa tcctattttt agatgcagcg
      361 accagccatt tggatattgc caatgaaaaa gcagtcaatg caaacttgaa tggcttgtct
      421 atcataaaaa ttatggcggc acacagaaag gaaacggtgg aatcagcaga taggaaaatg
      481 tctttaggat aaaaatacag tttcaaaaat actcaagact actgccgttt tttcgcctga
      541 gcgtcaaact ctgccagcgt catgttcaaa gtctgcaaac acggtgtcat taccgcatcg
      601 acagcttggt tcacatgatc cctttccaca ggcaacggac ggtaaacgaa gagcttgaag
      661 agttcgttca actcaatcga atccgccccc gttttcaaca cccaaccctg tctgccggaa
      721 tagatgtagc cgtgccgcgc cagcttttcc aaaagctcgc ccaactcgtc gtagcccata
      781 ttgatatgcc gtctgaactc ctgaacaggc aaggctttgc cttctttttg cgccgcatcc
      841 agaagcagca ggattttcaa cacgtcgtca aaccgtccgc gcgagtcgaa gcccctgcgg
      901 aacgcttctc cctgccagta ggagagtgaa gaagtcagca ccgcgccgcc caagaccagc
      961 gtccacaaca ggttcagcca caacagaaaa aacggcacgg cggcaaacgc gccgtaaatc
     1021 gagcggtagc cgtcgaaatt gcccatatac caagtgaaga gggagcgcgc ggtttccaga
     1081 caaaacgctg ttgccaaagc cccgacaaac gcctgccgcg cgggaacgaa gcggtttggc
     1141 acgaagcggt acagccccca cagcaaaagc gtcatgaagg tcagcgtcgc cgccgttcgc
     1201 aacgcgcccg accactgcgg cgcacctgag gcaagcgcgg catcctgtac cgagccgacc
     1261 ataaaggaaa tgcccacgcc caaagacagc ggcccgaacg tcagtaaagc ccaatagacg
     1321 agaaactgca tcatccacgg acgctgggaa ttgacccgcc agatgcggtt gaacgtattg
     1381 tctatcgtcc gaatcagcat cagcgaggta acgaccagca tcacgctgcc gattgccgtc
     1441 agccggttcg cctgctcgcg gaacgcattg atatagtcga acaccatgtc cgcgccctgc
     1501 ggcacaatgg tttggttgac gaaggagacg aacgaatccg accagcggtc gaacacgggg
     1561 aaaatcgaag cgaccgccac catcacggtc agcacgggga cgagtgccag cagcgtcgta
     1621 aacgtcatgc ttgccgccgc ctgcggtacg cgttcttcat caaagcggcg gacgacgaac
     1681 catgcaaacg cacagatttt attgtctgcc aaaccttgca aacgttgtaa aaaggtcata
     1741 atttcttgcc cggtcagtaa gttgggcatt gatgcccgat gttatagcca attttgccgt
     1801 caggaacaaa tgcctgaatt gcggctgttt cagacggcat cggaacaact gttatgccgt
     1861 ctgaagaccg aaccatttta acggaatccg cccatgaacc caaatccccc tcaaaatcct
     1921 cgtcctctac tatttccaaa acggcagcac ccgcaatccc gcccgccgaa tcgctcgcgg
     1981 catcgacagc gttgaaggtt gcgaagccgt attgcgcacc gtccccaaag tctccgccgt
     2041 ctgcgaagcc gtcaaaaaag atattcccga cagcggctcc cgtcctgacc gccgaagaaa
     2101 acaatatcgc cttcgcacaa agcaaacgct tggcggaact cgccatcaag tcggcataag
     2161 ccgcgtgttc agacggcatg gcgttcagat gccgtctgaa cgcgtttgcc tgtataatcc
     2221 gcgtctttac tgtccaactt cgcggttcgc aaacctcccg cgttaccaaa actaggattc
     2281 gatatgtcaa accaaaaagc cttggtcatc ttttcgggcg gtcaggattc gaccacctgc
     2341 ctgattcagg caatccaaac ctacgggcgc gaaaacgtcc aagccattac tttccaatac
     2401 gggcaacgcc atgccgtcga gctggaacgt gcccgctgga ttgcgcagga tttgggcgtc
     2461 aaacaaaccg tactcgactt gagcctgatg cggcagatta cgcacaatgc cctgatggac
     2521 gacaccgccg ccatcgaaac tgccgaaaac ggcgttccga atacctttgt agacggccgc
     2581 aacgcgcttt tcctgctcta tgccgcgatt tacgccaaag ggcaggggat acggcacatc
     2641 atcgcgggcg tgtgcgaaac cgacttctcc ggctatcccg actgccgcga cgtgtttgtc
     2701 aaatcgatga acgttaccct taatttggcg atggactatg attttcaaat ccacacgccg
     2761 ctgatgtatc tgaccaaggc gcaaacttgg gcgttggcgg acgaaatggg cgtgctggac
     2821 tatatccgcg agcaaaccca cacctgctat aacggcatcg tcggcggctg ccgcgaatgc
     2881 ccgagctgta tcttgcgcga acgcgggctg gcggaatatc tggaaagtaa aaaggccgtc
     2941 tgaacacgcg caaaccataa ggaatacgat atgcccaagc tccatatgtt ttacctcggc
     3001 ggcaatgccg gcaggtcgaa tatcgaagtg cacgacatcc aatttgctgt gtgcgacgac
     3061 taccgtgaag ccgtccccgc actcaaagcc gcgtggttcg gcgatgcgga caaaatccac
     3121 atcgacggct ggcaggttgt cgaatgggcg gacggttacg acatcgccgt atccgaaacg
     3181 cccaaaacga aaatgccgtc tgaaaacgcc ccgcgcttgt attttgccaa tgtcggcggc
     3241 tatcgcgcag ggcagctcgc cgaagcgcac gcttttgggc tgttcgccgc cgccacgcct
     3301 gccgaagcca aacaaaaagc cctgcaaacc ctgttgaccg acagctatgt tcagcaacat
     3361 aaagacaact taaaagacgt ggacaacctg cttgcgctcg accgcatcgg caatttccat
     3421 atccgcctga ccccgaatcc gcacggcaag cctgccgaaa tcggctttca gggctatttg
     3481 cccatttgag aacccatgaa aatcaccaaa atcttcacct tcgactcctc gcatatgctc
     3541 gacgggcatg acggcaaatg ccaaaacctg cacgggcata cctacaaact cgaaatcacc
     3601 gtttcagacg accccgtcag aggcgggccg aaagacggta tggtgatgga ctttaccgac
     3661 ttgaaagcca ttgtcaaaca acacattacc gaccccttcg accacgcctt catctaccac
     3721 ggcggcaacg ggcgcgaatc tcaaatcgcc gcactcttgg aaggctggaa catgaaaacc
     3781 ctgcgcctgc cctgccgcac cactgccgaa aatatggcgg tcgaaatgta cgaccgtctg
     3841 aaaaacgcgg ggctgaacgt gtgccgcgtc aaattgtggg aaacgccgac atcgtgtgcg
     3901 gagtatgaag gggagtaggg aatatcttga acgtatcgat atagtaaatt ccaataagac
     3961 atgcccaacc gcgtcattcc cgcgcaggcg ggaatccaga ccttgattta tcaggaatat
     4021 ttaaaaattg cagcaattcc aactctctgg attcccgcct gcgcggaaag gacggtttag
     4081 agcgtcctta tttgaattta ccgtaaaacg gttttttctc ctgtacggat tccccgtttt
     4141 ttcagacgac cttccatatc aaatacaccc attaaaagga atacccatga aactcctctt
     4201 catcccccta gtcctcttcg tcgccgccga acatttctac atcgcctggc ttgaaatgac
     4261 gcagattccc agcgaaaaag cggcggaaac gttcaagctg ccttatgaat ttatggaaca
     4321 aaatcgcgtg cagaccctgt tcggcaacca agggctgtat aacggctttc tcggcatcgg
     4381 gctggtgtgg tcgcggtttg ccgcgccgga taacgcggtg tacggcgcga cggtactgtt
     4441 tctcggcttc gtcctgattg ccgccgcgtg gggcgcgttc tcttccggca acaaaggcat
     4501 actcgtcaaa caaggtttgc ccgcattttt ggcagcggcg gcggtgttgg cggtatgaaa
     4561 aaaatcaatg ttgcccccga aaatccccaa taccgcatcg tcgaaatctt tgaaagcctg
     4621 caaggcgaag gctggaacac gggcataccc gccgttttcg tccgcttggg caaatgcaac
     4681 ctcgcctgcg gctggtgtga taccgattat ttgacattcg gcatgatgag cctgtccgac
     4741 atcttaggct gtctgaaaac ctatgccgcc cgcaacatca tcatcaccgg cggcgaaccg
     4801 accatacagc cgcatctcga tatgctgctg gacatgctca aggcggaagg ttatttcctc
     4861 tgtatcgaaa ccaacggact caatcccgcg ccgccgcaaa tcgactatgt cgccaccagc
     4921 cccaaagcct gctatgccgc caaatacgaa aaaagctgta tcgaaacagc cgacgaagtg
     4981 cggattgttg ccgacggcga tgtcgttgcg ttctgtgaaa atatggaacg caaaatccgt
     5041 gcgcatcatt actacctttc gccctgcgaa caaaacggtg caatgaacat ctacgacacc
     5101 atccgccaaa tcggtattct gaacagccgc cccgacgcgc cggtgcattg gcagttgagc
     5161 gtgcagacgc acaaatgggc gggaatagag tagtttaagc agtgtaactc aaagggacgg
     5221 cgtacggttt taccgatgtt tgacatacgg ggaaagtgtg ccgcttctgc gtggaaatgc
     5281 cggcatttcc accgcccaat caggacggag ccttactgaa taagatgctg ccgttgggta
     5341 caagctcggc ttcctaaatt ccgatggtct tttgaacctt gccgatactc tgtgccagtg
     5401 cgcgcaaatg gcagggttag ggaaaacgaa atgccgtctg aaacagcatt ctgagacctt
     5461 tgcaaaaata gtctgttaac gaaatttgac gcataaaaat gcgccaaaaa attttcaatt
     5521 gcctaaaacc ttcctaatat tgagcaaaaa gtgggaaaaa tcagaaaagt tttgcatttt
     5581 gaaaatgaga ttgagcataa aattttagta acctatgtta ttgcaaaggt ctcattctgt
     5641 ttcagacggc atttttctgt tgacgtcaaa aggaaaaacc gcctcggcaa tggataccga
     5701 ggcggtttgg atttggtcgg aatgagagga ttcgaacctc cgaccccttc gtcccgaacg
     5761 aagtgcgcta ccaggctgcg ctacattccg aattaagtaa ggcgtgatta tagcgcaaaa
     5821 aatacggcat gcctataccg ttttgccttt ttgccgcgtg tcgggcggat tgaaagggct
     5881 gtgtttgaat acagtgttga taagcatcct tatctccaag taattcaata agataacttt
     5941 ctacctgacc gaaaaaatca ttgcctttcc ctgacaaacg gttgaaatcg gcagattgtt
     6001 gaaacgcagc cgatttaaaa ggcctctccg acttttacgc cgcccgccgt gtcttgcggc
     6061 gaggcaagac cggcaacaaa ggcttgcgcc gcttggaaat ccgccgtctg tataacggct
     6121 tgcgcggcgg cactgccgag cgtgttggcc atatattgcc aacgttgcgc caaagcggga
     6181 ttgtcaggaa tgcggaaatc ttcgcgcagt tcatccacaa ggtcgggacg gttgcagacg
     6241 aggacgatgt cgcaacctgc ctcaaaggaa atgcgggcgc gttctttgat gccgcctgcc
     6301 ccgcacgcgc cctccatagt taaatcgtcc gagaaaatca cgcctttgaa tccgatatcg
     6361 cggcgcaaaa tttgtttgag ccagatttcg gaaaaccctg cgggctttgt gtccacttgt
     6421 ggataaacga cgtgggcggg cataaccgcc gccatacctt cgcggctcat aatgtggaag
     6481 ggggcgaggt cggcggcttc gagttcggac aggctgcgcc tgtcttccgg caagaccaga
     6541 tggctgtctc cttcgacaaa tccgtgtccg ggaaaatgtt tgccgcagga tttcataccg
     6601 ccttttgcca aacctttttg aagggcgagg gcgaggtggg cgaccgcttc gggattgtgg
     6661 tggaaactgc ggttgccgat gacggggcag tttccccagt ccaaatctaa gacgggcgtg
     6721 aaggacaaat cgatgccgca ggcggaaagt tcggttgcca aaacccagcc tatctgccgg
     6781 gctgcggttt cggcggcgga cgctccgtct ttgtcccaaa tttgccctaa aacgttcatt
     6841 gcgggcaggc gggtaaaacc ttcgatgaaa cgttgcaccc ttccgccttc gtgatcgacg
     6901 gcgataatga gttcgggtgt gcgcagggct ttgatttcgg cggtgagttc tttgagctgt
     6961 gcgatatttt ggaagttgcg gcggaacagg atgactccgc cgacggcggg atcgagaagg
     7021 cgttgctttt cctcttcggt caggcggaag gcggcaatgt ctgccatgac ggggccgcgc
     7081 ggaatatggg ggacggtcat tgcggtttgc tccaaaaagc ttcagacggc atatgccgtc
     7141 tgaacaggga aaggggtcag gcgttggcgc gttttttatc tttcaacaga aaaatcagca
     7201 ccgccaatac aatgcctgtc gtgccaaagc ccaacagcgc ggattttgtc agacccaatg
     7261 cgaggtagcc cgatgcggct gcggctgcaa ccgtcaaggc gtaaggcagt tgcgaggtaa
     7321 cgtggtcgat gtggttgcag cgcgcgccgg tggacgacag gatggtcgtg tcggagatgg
     7381 gcgaacagtg gtcgccgcat accgcccccg ccattactgc ggacatacac gggataatca
     7441 gcgcgggttc gactttgacc gccatggcgg cggcaatcgg cagcataatg ccgaacgtcc
     7501 cccagcttgt gcctgtggca aacgccatca cgctggcgag caggaagagg atgacgggca
     7561 ggaagccggg atggatgttg cccgcaacca gtgtggagag gtaatcgccg gtgtgcattt
     7621 cgccgacaac cgtactgatg agccaagcga ggattaaaat ggcgattgcg ccgaacatag
     7681 atttcgcacc ctgccaaacg gctttgggat agtcggcggt tttaatcgtg ccgagcgtgc
     7741 agagaacgac ggcaaggacg ccgcaagtgc cgccgaatac cagcgaagtg ttcacgtcgg
     7801 tattttcaaa tgcccccaaa atgctgaagg tttcgcttgc ctgtgcaccg gtgtagatca
     7861 tggcggaaac cgttgaggcg attaaggcca aaacgggaat aatcaatgcg taaaccctgc
     7921 cccagctgcc gtctgaaacg gcagtttcat cgtgggcttc gttcaacgcg gcttgttcga
     7981 aacgtgccat cgagccgatg tcgaaggaga accatgcgac gacgaacacc ataatcaggg
     8041 caaacagtgc gtaatagttc atcaggctca tggcgacaaa cgtccccatc ggcgtgtatt
     8101 cggtgatttt gtaggtaacg agcagtccgg caagcgtggc gataatcgac gcgccccagc
     8161 ttgaaacggg catcagcacg cacataggcg cggcagtgga gtcgaggatg taggcgagtt
     8221 tggcgcggga aactttaaac ttgtcggtaa cggggcgggc aatcgcaccg acggcgagac
     8281 tgtggaaata gtcgtcgata aaggttacaa acacgaggca ggcggtcagc attttcgcgc
     8341 cgcgccggtt tttaatgtgc cgttttgccc agtcggcaaa cgcctgattg ctgccggagt
     8401 aggtcagcag ggaagtaaaa atacccaaaa gtatcaggaa aaccaagatt tttggtttgc
     8461 ccagcgacca atcgccgtct gaccaagcca agccgacgac catgtctttc aggtgtgtca
     8521 gaccgtcgac ggggttgccg ccgaccaaaa aggcaacgcc gaccagaata ccgatgccta
     8581 aagacagcag tacgcggcgg gtaatgacgg caagtgccag tgccaaaaag ggtggcataa
     8641 ccgagaaaaa tgaatgtgaa tagtcgatca gctgcatggt tatgggggtg ttaagcgtcc
     8701 ggatgggagc gtatctgtcc gcctccggtt tgggttttgt tggcaaaatg ggcggaaata
     8761 ttttttgtcg taaaaaatat ttgtttaaaa tcaaccaact gatttttgta aaatgcccgt
     8821 taatcggtat tgacgggcat tttatcattt aaaaaatatt ttggttaaat tatgtgggtt
     8881 attgcaggtt taatgcgata aacagcgtgt tgccacggcg catgaccagc agggggacgt
     8941 ttttgcctgc cttgtccata gctttgcgga aaccggcttc gtcattgacg gggacttgcc
     9001 cgacggcgag gatttcgtcg ccgcgcctca agcctgcgcg ttctgccgcg tcggaaaccc
     9061 gtacgacgac gaggtgtccg ccgctgctgt cggtatgtgt ctgaagggta atgcctgcgg
     9121 attcgaccga gaacgtaccg gattgctgtt cggtgtaggg ggcttcatct gttttggatg
     9181 atgcgccgat atgctcggcg gcgttgccca gcttgacttt gattgtgatt tcttcgcctt
     9241 tgcgccatac gccgaggctg acttcttttc ccggcgtaat ggcgccgacc ataacgggaa
     9301 ggtcgccgga agaacgtatt tctccgccgt cgaggctgag gacgatgtcg cctgcctgca
     9361 agcctgcacg ttctgcgggg ctgccgggaa ggattttggt aatcaatgcg ccgccggctt
     9421 tgtccaaacc gaacgattgt gccaaaccgt aggatacttc ttgaataatc acgcccagtt
     9481 gtccgcgttg gactttgccg gtgtttttca gctgttcggc gacattcatg gcaacgtcaa
     9541 tcgggatggc gaaggaaatg cccatgaatc cgccgctgcg gctgtatatt tgcgagttga
     9601 tgccgacgac ctgtcctttt aagttgaaca gcgggccgcc ggagttgccc ggattgatgg
     9661 caacgtcggt ttggatgaag ggtgtgtagc tttcgttggg caggcttctg cctttggcgg
     9721 acacgatgcc ggcggtcacg ctgttgtcga agccgaaggg cgcgccgatg gcggcgaccc
     9781 attcgcccgg tttcaaatct ttgggattgc cgattttgac gacgggcagc tcttccgttg
     9841 cgtcgatttt cagaagggcg acatcggatt ggacatccga accgatgagt ttggcggtat
     9901 attcgcgctt gtcgttgagc aggactttga tactgcccat gccggtaacg acgtgcgtat
     9961 tggtcagaat atagccgtct ttgctgatga tgaagcccga accgaagttc aatccgccgt
    10021 catctgcttc ttcttggggg atttcgggca tgttcgggac gaggcgtttg aaaaattcgt
    10081 agaacgggtc gctgtcggca agcgggtcgg aatcggtttc ggcattgccg ctgccgtttt
    10141 gggtgcgcgg ggcgggggct gcctgaatat tgacgactgc cgggccttcg ctttgaacca
    10201 gttggacaaa gtcgggcagc agcatactga cgctgccgtc gtctttggtg tgtttgatgc
    10261 gttctacgaa ggatgcttct tttttgtccg caccgaaaaa gctgccggct ttgtcgcagc
    10321 ctgccagcga ggcggcacac agtgctgcca aagcgaggta ttggtatttt ttgaacacgt
    10381 tttgtccttt gtcggatgcc ggtaccggct ttaatgccgt ctgaagcgca ttttgtcggc
    10441 ttcagacggc ataggttgaa attctacaac gtccgtccga attttcaagc gtttcatttt
    10501 gaagggcggc ggcagtcagg ctttggcggg atattcgcac aaatcgttga tgatgcaggt
    10561 ttggcattgc ggtttgagtg ccttgcaggt gtagcgtccg tgcaaaatca gccagtggtg
    10621 cgcgtccatc agaaattctt taggaatgaa gcgcatcagt ttgtcttcga cttcgcgcac
    10681 atctttcccg ggggcgattt tggttcggtt ggacacgcgg aaaatatgcg tatcgaccgc
    10741 cataacgggg tgtccgaacg ctgtgttcaa taccacgttt gccgttttgc gccccacgcc
    10801 cggcaacgat tccaaagcct cgcggtcttc cggcacttcg cccttgtatt tttccagcag
    10861 gatgcggcag gtctgcatga tgtgcttgga cttggtttta tacaacccga tggttttcgt
    10921 gtattccatc acgccgtcca agcccaaatc cagcattgcc tgcggcgtat cggcaaccgg
    10981 aaacagcttc gccgtcgcct tgtttacgcc gacatcggtc gcctgcgctg aaagcagaac
    11041 ggcgattaaa agctcgaaag gggagttgaa attcagctcg gtggtcggat gggggttggc
    11101 ggcgcggaaa cgctcgaaga tttcttggcg gatttgtctg ttcatttttt tatacggtag
    11161 gtttgtgcgg tcggcattat aacgcacggt tcaggcggcg taatattgca ttccccacag
    11221 gataaaggcg taacgcgcca tcttgccgac aaccagcatc agcccgcttg tccacggatt
    11281 caaccgcagc cagccggcgg caagcggcag tgcgtcgccg acgacgggca gccaggcaaa
    11341 cgcaagcagc caaataccga aacgccgcat cagattcagt gttttttcag acggcatttt
    11401 tcgggaaggc agcaaacgtc ccatccaata ggaaaccata ctgcccaatc cgttggcaag
    11461 gccggcgcac agcaacgcgc cgtatgcgtg ttcgggaaag cggtggacga acagggcaaa
    11521 ggcggcttcg gatgtgccgg gcaggagggt ggcggaagtg aaggcggaaa aggcgagtgc
    11581 ggcgtaggcg ggcatcattg caaacagtct caaacaggca aaaatcggcg acggattgta
    11641 cg
//
LOCUS       DAXHTY010000061        11001 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXHTY010000061 DAXHTY010000000
VERSION     DAXHTY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11001
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.061"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            228..410
                     /locus_tag="ACFNMK_001401"
     CDS             228..410
                     /locus_tag="ACFNMK_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364661.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(472..1473)
                     /locus_tag="ACFNMK_001402"
     CDS             complement(472..1473)
                     /locus_tag="ACFNMK_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC6364662.1"
                     /translation="MIDGQTTEPKQKNRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEVGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1550..5383)
                     /locus_tag="ACFNMK_001403"
     CDS             complement(1550..5383)
                     /locus_tag="ACFNMK_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC6364663.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEFVELAGLDGKHPIIHCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKHTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAISPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKARWEWLLANLDAPLADYKARYGAA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDADMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKASVGKA
                     PIKEQVIHFINRPLPKNVPAKTPRSLLGIEDGKSIPIIRNPASPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVQLNGVQGQQ
                     YLYHDPCHTPIKTMNATQLASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5644..5740
                     /gene="ffs"
                     /locus_tag="ACFNMK_001404"
     ncRNA           5644..5740
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFNMK_001404"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5908..7599
                     /gene="dld"
                     /locus_tag="ACFNMK_001405"
     CDS             5908..7599
                     /gene="dld"
                     /locus_tag="ACFNMK_001405"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC6364664.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPTKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7731..7838
                     /locus_tag="ACFNMK_001406"
                     /pseudo
     CDS             7731..7838
                     /locus_tag="ACFNMK_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7894..8265
                     /locus_tag="ACFNMK_001407"
     CDS             7894..8265
                     /locus_tag="ACFNMK_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC6364665.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8420..8968
                     /locus_tag="ACFNMK_001408"
     CDS             8420..8968
                     /locus_tag="ACFNMK_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6364666.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            9103..10194
                     /locus_tag="ACFNMK_001409"
     CDS             9103..10194
                     /locus_tag="ACFNMK_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6364667.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNSYLDDGYDSLKARLD
                     GAWAETERLADLAWDGAPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10766..10936
                     /locus_tag="ACFNMK_001410"
     CDS             10766..10936
                     /locus_tag="ACFNMK_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC6364668.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2581 a         3007 c         2973 g         2440 t
ORIGIN      
        1 gcaatcatgg cttgggcggt ttgctggaag aaggtgctca tgagaaatcc cctaaatgtc
       61 ttggtgggaa tttaggggat tttggggaat tttgcaaagg tctctatttg tactgtcttc
      121 ggctcgccgc cttgtcctta tttaaattta attcactata tttgcccgtg cgtttgtgat
      181 aaattcggca ctgtatttga tatgatttta acaaatcggg aggggcaatg ttttggtatg
      241 taatcggctt ttgctccttt gtcgtcgcgc tgctgtcgct gtgggtcaat gtcggcgcat
      301 tcggtatgca ggaagacgat accccgcaat cggattatga aaaacgtttg ggcttgggtg
      361 cgaagctgaa aaacaaaaat acgccggata cggaagaaaa gcagcaataa agatatgaaa
      421 tgccgtctga aagattttca gacggcattt gctttgaagc atgggagaca gtcaaacatt
      481 tatctcatgc tcaaatgcgt tcaggattcg gcggacttca tccgcatcct taaccgttcg
      541 gacgcggtca aacagcgttt gcgccgggtc gaacgttttt ttcatcatgc ccaaccattg
      601 cttaagacgg gcgatcgggt atttattgtt tgcctctttt gtcaggcaca gctcgaaaaa
      661 ctgccgtatc catttagaaa cttcggcaaa atccgtgtct ttgaccggcc cgccgttctc
      721 gtattgcttg atttgccgcg ccaaatcggg gcggatgacc gcgccgcgac cgagcatcac
      781 gctgttgcag ccgctgattg ttttgatgcc gatatagtct tgcaggctga aaacgtcgcc
      841 gttggcggta acgggaatat tgacgctgtc tcggattttc cttatccatt cccaatgcgc
      901 cggcggttcg taaccctcgg ctttggtacg cgcgtgtacg gtcagtccgc atgcgcccac
      961 ttcggcaatc gcacaggcgc attccaaagc ccggcttttg tcttcgtaac cgagccgcat
     1021 ttttgcggtg agcggaatat gtgcgggcaa acgtccgcgc agcgttttga cgatgtggaa
     1081 tatcagttcc ggctctttta aaaggattgc gccgcctttg tgtttgttga cggtgggcgc
     1141 ggggcagccg aagttcaaat cgattttgtt cgcaccgaag cggacggctt ccagcgcatt
     1201 cgccgccata ttgtccgcat cgctgcccaa aagttggacg gtgcagggcg tgccggaaaa
     1261 cgttttgttt ccgttggcga tttcggggac atattttaac catatggatc gggaatgcac
     1321 ggtatgggta atgcgtacaa attcgctgac gcattcgtcg tagccgccaa tacgcgtcag
     1381 caggtcgcgc atcacgtcat cgaccagacc ctgcataggg gcaaggatga ttcggttttt
     1441 ttgtttgggt tcggttgttt gtccgtcaat catgatttgg gtaggtattg ggtttaaaca
     1501 atgccgtctg aaggctttcg gcgttcagac gacatttccg tgtccgttgt tacagcaata
     1561 ctttttccac gccgccgtta ttggcttttt tcacaaactc gtccagccag ttttcgccga
     1621 ggatgtgttt cgccatttcg atgacgatgt agtctgcagg catattgttg tcgtcggcgt
     1681 agcggctcag accttgcagg caggcggggc aggaggtgag cattttgacg ggttcgccct
     1741 gcggcagctc tttgaggttt ttctcgattt cctcttgttt gcggaacttg acttgcgtgg
     1801 cgatgtctgg tcgtttgacg gcgaacatac cggactcgcc gcagcagcgg tcgcttaaga
     1861 cgactttctg tcccatcagg ctgctggcca gttgggtggc attcatggtt ttgatgggcg
     1921 tgtggcaggg gtcgtggtag aggtattgct gaccttgtac tccgtttagt tgcacgcctt
     1981 tttcgagcag atattcgtgg atgtcgatga tacggcagcc tgggaagatt tcctcaaaac
     2041 ggtatttttc cagttggtcg taacaagtgc cgcaactgac gacgacggtt ttgatgtcga
     2101 ggtagttgag ggtgttcgcc atacggtgga aagcgacgcg gttgttggtg ctcatttctt
     2161 cggcttttgc cttgttgccg ccggcatctt gcggatagcc gcaacacata taacccggcg
     2221 gcaggacggt ttgtacgccg acgtgccaaa gcatagcttg gacggcaagc ccgatttggc
     2281 tgaacagacg ctccgagccg cagccgggga agtagaacac ggcttcggca tcttccggag
     2341 aagccggatt gcggatgatg gggatgctct tgccgtcttc gatgcccaat aaggagcgcg
     2401 gtgttttggc gggtacgttt ttgggcagcg ggcggttgat gaagtggata acctgttctt
     2461 taatcggggc tttgccgacg cttgccttgg gttcggcttt ttgcttttta gtgccgatcg
     2521 gaagaagttt gccgattttg taggcgaagt tctgcgcggg gaagccggtc tgtatcatcg
     2581 cggctcgcag ggctttgatg gttttcgggc ctgtggcgtt caaaaatgcc atgcccattg
     2641 aagccgcagg cgcaaatcgt ttgtggccgg aatcggcaag gtagttgcgg acggctacgg
     2701 taacgtcgcc gaagtcgata ttgacggggc aaggcttaac gcagcggtgg cacacggtgc
     2761 agtggtcgcc gatgtccatc agttcttcaa aatgtttgac ggaaacgccg cgccgggttt
     2821 gctcttcgta taagaaggct tcggtcaaaa gccctacgcc gaggattttg ttgcgggggc
     2881 tgtacagcag gttggcacgt ggaacgtggg tggagcagac gggtttgcat ttgccgcagc
     2941 gcaggcagtc tttgacggaa tcggcaatcg tgccgaggtc tgatttttcc ataatcagcg
     3001 attccgcgcc caaaagctcg aaagacggcg tgtaggcgtt gcgtaagtcc gagcctttca
     3061 tcagtttgtg gcggttgaag gtgtgtttcg gatcgacttg gtttttgtag ttccaaaacg
     3121 gctgcatttc ttcgtcgctt aaaaattcga gcttggtaat gccgatgccg tgttcgcccg
     3181 aaatcacgcc gttaagcgaa cgggcgattt tcataatgcg ttcgactgag cggtatgccg
     3241 tctgaagcat gtcggcatcg tctgaattga cggggatatt ggtgtggacg ttgccgtcgc
     3301 cggcgtgcat atgcagcgca acgaagacgc ggctgcgtac ggttttggcg tggatttttc
     3361 ccaaaccttg gataattttg gtatcggttt tgccgctgaa gatttcagaa agcggcttca
     3421 ttacgtccgc tttgacagac acgcgcaggc ggaaatcgcg gaatgcggta aagcagcttt
     3481 cattgtcttt ggcttcgggc gcggcatgga cggctgcgcc atagcgagcc ttatagtcgg
     3541 caagcggcgc gtctaagtta gcaagtagcc attcccaacg cgctttgaca gcggcaacgt
     3601 gggcaagggc gtgtttgccg cgttcgccca acagttcggc ggttggtagg tctgttccca
     3661 ttttgtcgat ggggagtttg ccggacagat attgctccaa ggcggcacag agtttgagtt
     3721 tgttttgaat ggaaagctca atgttgatgc gttcgatgcc gtctgaatac tcaccgagcc
     3781 tttcgagtgg gatgaccacg tcttcgttga ttttaaaggc gttggtgtgt ttggcgatgg
     3841 ctgcggtgcg gctgcggtcg agccagaagg ttttgcgtgc ttcgggagat atggcgataa
     3901 agccttcgcc gtcgcgggcg cgggcaagtt cgcagatgtg ttcggcggct gcctctacgg
     3961 cggcttcgtc gtctgaaacc acgtctgcca gcaagaccat tttcggccgg cctttgcctg
     4021 ccgctttggt ggcgtagccg acggcgcgga cgtaacgcca gtccaaatgc tccaaaccgg
     4081 ccagccgcac gctgtcgtgg gcgagcagga aatcgcggat ttcgacgata gaaggcgtag
     4141 cggtggcgac cgtaccgaaa aactccatac acacggtgcg cgtgtgtttc ggcattttgt
     4201 gcaacacgaa ggcgacgctg gtgatgatgc cgtccgtgcc ttctttctgc acgccgggta
     4261 ggccgctcaa gaatttgtcg gtaacgtctt tgcccaaacc gactttgcgg aatttgtggc
     4321 cgggaatttc caaacgttcg gttttaacaa tattgaggcc gtctgaatcc agcgtgtgaa
     4381 cgtcgaacac ggcggtttct tcgtcgtgga ttttgccgaa attatggcgc acgcgctcga
     4441 tgcgcagcca ttcgccttga gggttgacca tctgccagta ggcgaggttg tccaacgccg
     4501 tcccccacag tacggctttt ttgccgcccg cgttcatcgc cacattgccg cccacacagg
     4561 acgcatcggc ggaagtcgga tcgacggcga acaccaaacc cgcctgatgc gcggtttctt
     4621 ctacccgccg cgttaccacg cccgcgccgc aatggataat cggatgtttg ccatccaagc
     4681 ccgccagttc gacaaactcc acgccgcgat gtttgtccag cttttccgta ttgatgaccg
     4741 cgctcattgc gtccaaagga atcgcgccgc cggtataacc cgtaccgccg ccgcgcggga
     4801 taataaccaa gtccaactca attaaggcgc gcaccaaagg cgcgacttcc gcctccgtgt
     4861 cgggattgac gacgacaaac ggatactcca cgcgccagtc ggttgcatcg gtaacgtgcg
     4921 ttacccgcgc cagcccgtca aacataatat tgtgcggctt ggtgattttg ctcaaacgct
     4981 ccaaaatctg ccgccgcttt tggctggttt catcaaaact gctatcaaaa cgctcgactg
     5041 ctttttctgc tgcggcaacc aacacatcca cttgccgatt atcgtcgcgg cgtttgcgga
     5101 tttcatttaa gcggtggcgc atttcccgca ccagcgcggc gcggcgtttc gggtgctcca
     5161 acaagtcatc gaccagatat ggattgcgca caactaccca aatatcgccc aacacttcaa
     5221 acagcatccg tgccgaacgt ccggttttac gctgtccgcg caagtcttgc aggatttgcc
     5281 acgcttcgtc gcccaataat cggatgacga tttcgcggtc ggtgtaggaa gtatagttgt
     5341 aggggatttc ccgaatacgc tgaggggcgg tagtcgtggt catggtgtgt ccgtgtttgg
     5401 cagtttttat ttgaatgccg tctgaaaagt cggtttcagg cgagtttgaa aaggcgataa
     5461 tgtagttcaa tttttgatac ttttcaatgc cgaaacagga aataccctta tagaaacaaa
     5521 ataacgcttt gagaaattgt tgacaatatt atcaaatata aattttaggt ggtgtttccg
     5581 gcgtatgacc gtatcaaaac ggttgagttt ttcagaaaac ccgttataat gccgtttctc
     5641 tgaggcgggt ctccccgcat ggcaaatcgg aacaccgggt caggggcgga agccagcagc
     5701 ccactccgat gcgccagtgc cgggggtttg gcccgccgcc ctatttgaaa cgccggagct
     5761 tcgatgttcc ggcgttcccg tatcgcaccg atgccgtctg aaagccgttc agacggcatt
     5821 ttctttactt tgccataacg tataactaca atgtgaaaac aattccaatg tgcatatgca
     5881 cctgccgaca attttaagga aactgccatg agcgcatcac aattactgtc aagactgacc
     5941 caaaccgtag gcgaaaaata catcattacc gaccccacga aaaccgaaca ataccgccaa
     6001 ggctaccgct tcggcgaggg taaggcgttg gcggtggtgc gtcccggaag cattctggaa
     6061 atgtggaaaa ttctgcaggc gtgcgtcgaa gcggacgtga ttgtgattac gcaggcggcg
     6121 aataccggcc tgaccggcgg ctcgaccccc gacggcaacg attacgaccg cgacatcgtg
     6181 attgtgaaca ccatgcggat gaacatcatc caaaccatca acaacaacga acaagtcgtc
     6241 tgcctgcccg gctcgaccct gaaccagctt gaattgctgc tgaaaccttt gggacgcgaa
     6301 ccgcattcgg tcatcggctc atcctgcatc ggcgcgtccg ttttaggcgg cgtgtgcaac
     6361 aactccggcg gcgcgttggt acagcgcggc ccggcctaca ccgaaatggc actgttcgcc
     6421 caaatcaacg aagaaggcag gttggaactg gtcaaccact tgggcataga cttgggcgac
     6481 acgcccgaag aaatcctgac caacctgcaa ggtcatcatt atcaaaacaa agacatcaaa
     6541 caagacgcgg gcaaagggca cgaccacgcc tattgcgaac acgtccgcca agtggacgaa
     6601 ccgaccgccg cgcgtttcaa tgccgacccc gcccgccatt acgaagcctc gggctgcgcg
     6661 ggcaaactga tggtttttgc cgtccgtttg gacaccttcc cgcaagaaaa acaaaccgcc
     6721 gtgttctaca tcggcacgaa cgacatcaac gagctgaccg acatccgccg cgccgccttg
     6781 ggcgaatttg aaagcctgcc tgtttccggc gaatacatcc accgccacgc tttcgacatt
     6841 gccgacgtgt acggcaaaga cacgttttac gtcatcaaaa aattcggtac gcaccaactg
     6901 ccgaaattat ttgacttgaa ggcgcgcgtg gacaggttcg gcaaaaaagc cagcttcctg
     6961 cccaaacatt tttccgacaa ggcaatgcag ttcgtcagca aatacctgcc cgaccacctg
     7021 cccaaatcca tgcgcgatta ccgcgacaaa tacgaacacc acctgattct gaaaatgggc
     7081 gggaaaggcg tggatgaggc gcgcgcgttc ttaaaagaat attttgcaca ccacagcggc
     7141 gcgtttttcg agtgcaacgc cgaagaaacc caagccgcga tgctgcaccg tttcgccgtc
     7201 gcctccgccg ccatccgcta ccgcgccgtg catgatgacg aagtggaaga cttggtcgcg
     7261 ctcgacatcg ccctgcgccg cgacgatcgc gactggtttg aaaaactgcc gtcggaaatc
     7321 gacaataaaa tcatccataa actctactat ggacatttca tgtgccacgt tttccatcag
     7381 gattacatca tcaaaaaagg caacgactgc atggcgttgg aacacgaaat gctgcatctt
     7441 ttagaccaac gcggcgcgca atatcccgcc gaacacaacg tcggtcattt gtatgaagcc
     7501 aaacccgcgc tcaaacagtt ttaccgcaaa cttgacccga ccaacagctt caacccgggc
     7561 gtaggcaaaa ccagcaaaaa gaaaaactgg gcggaataag cgcgtccgct ttgaaggcag
     7621 gcaatgccgt ctgaaggcaa aacgccgttc agacggcatt gtcgggtttg aagttccccg
     7681 tcgggtaatc gctttcaaac cgtatgccga cacacgggcg cgcccctccc aaatatagtg
     7741 gattaaattt aaatcaggac aaggcaacga agccgcagac agtacagata gtacggcaag
     7801 gcgaggtaac gccgtactgg tttaaattta atccactata aagtctgatc ctaccttccc
     7861 aaagggcagg gtttcaaccg aaaaggaaac acgatgaaac catacaaaat ctacacccat
     7921 cccgccctgc cgccccaagc cgtcaaacaa ggctggtcat ggccggggct cttgttcggc
     7981 acgctgtggg cgtgtttcaa aagaatgtgg ggtttggggc tggggctgac gggcgcgata
     8041 ttcgtgctgg ctgtgtttgc ccaactggtt tacggcgaca cgcccgccac agactcggcg
     8101 tttaacgtat tgggcttggc ggtttccgtc tggttcggcg caaagggcaa cagcctttat
     8161 gcacgccacc ttttatcgcg cggctatacc gaattgcccg aaacggttga ggcagccaat
     8221 ccccaagccg cattggcgca atatttcggg cgcgggggcc gataagcgcg ttttcttttc
     8281 catcgtgccg tctgaaaaat gttcagacgg catttcttat aacctttcgt ttgcagcaca
     8341 taaccaatca gtctttccta ttccgttccg aaatgcatac catgcgcccg tacccaatat
     8401 ttgcgaacaa ggaaagaaaa tggcacgttt aaccgtacac accctcgaaa ccgcccccga
     8461 agccgccaaa gcgcgcgtcg aggcggtact tcaaaacaac ggctttatcc ccaaccttat
     8521 cggcgtatta tcaaacgccc ccgaagcctt ggcgttttac caagaagtcg gcaagctcaa
     8581 cgccgccaac agcctgaccg ccggcgaagt cgaagtaatc cagattattg ccgcccgcac
     8641 caaccaatgc ggcttctgcg tggcagggca caccaaactc gcaaccctga aaaaactcct
     8701 ttccgaacaa tccgtcaaag ccgcgcgcgc tttggcggca ggcgaatttg acgatgctaa
     8761 actcggcgcg ctcgccgcct ttacccaagc cgtaatggca aaaaaaggcg cggtatccga
     8821 cgaggaactc aaagcatttt ttgatgcggg ctacaaccag cagcaggcag tcgaagtcgt
     8881 gatgggcgta gccttggcaa ctttgtgcaa ctacgtcaac aacctcggac aaaccgaaat
     8941 caaccccgaa ttgcaggctt acgcctgata cggcaaaccg cccgaatatc gggcggtttt
     9001 tcaaaaacac cccctcaaac aaaaacaagc cgcctaagcg gcagggaagc agccgcgcat
     9061 caggcgcgcc cccgatttcc ccgcaaacct taaggaacaa agatgaacgc ccaaaccctg
     9121 attgccaacg ttgccgaatt cgtcaaaacc aagctcaaac ccatagtgga cgacatcgac
     9181 cgcaaaggat actaccccga agcatttatg cgcgaactcg gcgcaatcgg tggattcggc
     9241 gcagtcggta cagaagccga aggcggcaac ggcttgggtt tggcaacgca aatcgccgta
     9301 ttgcgcgaaa tcggcaaaga atgcggcgca acctctttca gtgcgtggtg tcaggcggct
     9361 tgcgcgtggt atctgcacca aacgcccaac cgggccgtca aagacaaata ccttgccgac
     9421 atcctgcaag gcaaagtatt ggcgggtacg ggcatgtcca ataccgtcaa acaccttgcc
     9481 gacatcgaaa aacacaacct ccaagccgag cgcgtggagg gcggctacac agtcaacggc
     9541 gcgctgccgt gggtgtccaa catcggcgaa gaccacattt gggcgaatac cgcccaaatc
     9601 ggcggcggct acgtcatgtt catcacaggc ggacaatggg aaggcgtaag cctgcaaaac
     9661 tgccccgaat tttgcgccct cgaaggcaca cgcaccttca gcctgaactt caaagacgta
     9721 tttatccccg acgaagacat catcgccgcg cccgaacagt ttgccgacta catccaaagc
     9781 atcaaagcag gctttatcct cctgcaaatc ggcatcggcg cgggcgtgat tgacggcagc
     9841 ctcggcatca tccgcctcgc caacgtcgtc aacgccgaag tcaacagcta tctcgacgac
     9901 ggctacgaca gcctcaaagc aaggttggac ggcgcgtggg cagaaaccga acggctcgcc
     9961 gacttggcgt gggacggcgc gcccgataac ctcgccaccc tcaagctgcg cgaagccgcc
    10021 gccgtactgg cacttgccgc cgcccaatcc gccgccctgc attccggcgc aaaaggctac
    10081 ctgatgcgca gtcccgccca aaggcgcgtc cgcgaagcaa tgtttgtcgc catcgtaacc
    10141 cccgcgatca aacacctgcg caaagaaata gcggcaatcg aagccgcaaa ataaacaggc
    10201 ggaaaaaaca gaaaccccca ccgcgtcatt cccgcaaaag cgggaatcca gcccccaacg
    10261 caacaggaat ccatcggaaa aaacagaaac cccaaaaccg tcattcccgc aaaagcggga
    10321 atccagcaaa cgaaaaatca caggaatcca tcggaaaaac agaaaccaaa cagacaagat
    10381 tcccgcctgc gcgggaatga cgcgggagcg ggggtcgtca ttcccgcgaa agcgggaatc
    10441 cagcccccaa cgcaacagga atccatcgaa aaaaccgaaa cctccaaacc gtcattccca
    10501 cggaagtggg aatccagtaa tcgaaaaacc acaggaatct atcgaaaaaa aaccaacccc
    10561 aaaaaaaccg ggcggatacc gcaccatcct cccaaaccct gatttatagc ggatgaacaa
    10621 aaatcagggc aaggcggcga agccgcagac agtacaaata gtacgggtgc ttcagtactt
    10681 tagagaatcg ttctctttga gctaaggcga ggcaacgccg taccggtttt tgttaaccca
    10741 ctatacaaaa aacaaggaaa cacaaatggc gcaatatatg tgcggcccct gcggctggat
    10801 ttacgatgaa gaacacggcg accccgaaca cggcatcgcc cccgggacaa agtttgaaga
    10861 catccccgac gactggaaat gccccgaatg cggtgtaggc aaagaagatt tctacctgtt
    10921 ggatttcgtg atatagcggc agccgcaccc ttagggaaaa ccccgtcatc cggcgttccg
    10981 cctgcggcgg cagggaacac c
//
LOCUS       DAXHTY010000062        10657 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXHTY010000062 DAXHTY010000000
VERSION     DAXHTY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.062"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(92..1234)
                     /locus_tag="ACFNMK_001411"
     CDS             complement(92..1234)
                     /locus_tag="ACFNMK_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC6364669.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDNDRRIVQTALDNARSA
                     EVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRVLQALYPQLGTWLKKYYAG
                     WLAAMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1283..1705)
                     /locus_tag="ACFNMK_001412"
     CDS             complement(1283..1705)
                     /locus_tag="ACFNMK_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364670.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1698..2507)
                     /locus_tag="ACFNMK_001413"
     CDS             complement(1698..2507)
                     /locus_tag="ACFNMK_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6364671.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCNDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2589..2966)
                     /gene="acpS"
                     /locus_tag="ACFNMK_001414"
     CDS             complement(2589..2966)
                     /gene="acpS"
                     /locus_tag="ACFNMK_001414"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC6364672.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVIAEK"
     gene            complement(3166..3933)
                     /locus_tag="ACFNMK_001415"
     CDS             complement(3166..3933)
                     /locus_tag="ACFNMK_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6364673.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMILFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3943..4482)
                     /locus_tag="ACFNMK_001416"
                     /pseudo
     CDS             complement(3943..4482)
                     /locus_tag="ACFNMK_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
     gene            complement(4654..4875)
                     /locus_tag="ACFNMK_001417"
                     /pseudo
     CDS             complement(4654..4875)
                     /locus_tag="ACFNMK_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine catabolism dioxygenase TauD"
     gene            complement(4874..4981)
                     /locus_tag="ACFNMK_001418"
                     /pseudo
     CDS             complement(4874..4981)
                     /locus_tag="ACFNMK_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(5027..5755)
                     /gene="pdxJ"
                     /locus_tag="ACFNMK_001419"
     CDS             complement(5027..5755)
                     /gene="pdxJ"
                     /locus_tag="ACFNMK_001419"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC6364674.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAEFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(5779..6570)
                     /gene="recO"
                     /locus_tag="ACFNMK_001420"
     CDS             complement(5779..6570)
                     /gene="recO"
                     /locus_tag="ACFNMK_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC6364675.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVSFVPVSASWYGSQELKTLHRAEWVGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHTDGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(6604..7731)
                     /gene="pheA"
                     /locus_tag="ACFNMK_001421"
     CDS             complement(6604..7731)
                     /gene="pheA"
                     /locus_tag="ACFNMK_001421"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC6364676.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASSSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRQDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(7770..9002)
                     /locus_tag="ACFNMK_001422"
     CDS             complement(7770..9002)
                     /locus_tag="ACFNMK_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFC6364677.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
     gene            complement(9522..10376)
                     /locus_tag="ACFNMK_001423"
     CDS             complement(9522..10376)
                     /locus_tag="ACFNMK_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6364678.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT         2551 a         2876 c         2743 g         2487 t
ORIGIN      
        1 atcctgattt ttgttaatcc actataatat cggccggata ttaaaaaaag ccgtctgaac
       61 atttttcaga cggcattttt gtacactttc ctcaacggtt cgatccctgc accatatcaa
      121 tcaggaacag gcggcaatcg aggttgccgt tataaagcgg aattttgcgt tttggcgaca
      181 ggcgcatgaa tttgggcatt tcccgatctc cggtaaacat tgccgccaac cagcctgcgt
      241 aatatttctt caaccacgtc cccaactgcg gataaagcgc ctgcaagaca cggacttcct
      301 caaggcgcac gccgtagggc ggattggaca ccataatgcc gttttcgccg ttcggtcgga
      361 cggactgcgc gtcggcaacg ctgaaggaaa cgatgtcgtc cacttcggca ctgcgtgcgt
      421 tgtccaatgc cgtctgaacg atgcggcggt cgttgtcgct gcctgcaatc ggggcgcgga
      481 cggggcgggt ttgcgcttcg gcgcggcggc gcaaatccgc ccacagcgtt ttatcgaaat
      541 tttgcagttt ttcaaaaccg aagcggcgca tcatgcccgg cgcgcggcgg gcggcaatcc
      601 aagcggcttc gatagcaatc gtgccgctgc cgcaaaacgg gtcttgaaac ggctgcgtgc
      661 cgtcgtagcc tgccgagagc agcagtccgg cggcaaggtt ttcgcgcagc ggggcttcgc
      721 cggtatccag gcggtagccg cgtttgaaca gggcttcgcc cgaagtgtcg atgaagattt
      781 caacattgcg ttcgtccaaa aaggcgtgga tgcggacatc gggcgcggct ttgtccacgc
      841 tcggacgtgc gtcgtaaatg tcgcggaaag cgtcgcagac ggcatctttg acggtcagtc
      901 cgacaaattg gaggcttttg acgttggcac gctttgcctc gactttgact ttgaacgtct
      961 gctgtaaagt aaaccaatta aaccagttga tgtttttggc aagtttgtag atgtcgcgct
     1021 cggttcggta tgtccctttg gtcagacgca gcaggatacg gccggcggta cgcgagtgca
     1081 ggttggcggc gtaggcctgt tccaatccgc cccggcagga aacgccgccg tcaaacgctt
     1141 gtacatcggt acagccgagg ctttcgagtt cttgcgataa aacggtttcc aagccgcgcg
     1201 ggcaggtggc gaaaagtgta tataaagtat tcatagcgtg tcctttccgg acagatgttc
     1261 cggcaatagc ggattgaaag gtttattcgg cggtaaatgc cgtctgaaat gtccgactgc
     1321 cgattgtaat gtccgccgta aaatcgcgcc tgccttcgac acagatgggc aggcggatgc
     1381 gtactgcctg ccaagtcccc gacggttgcc gctcgaacat atagcggttg aaacccatat
     1441 ccatattttt catactgaag ctgatgctga cctgttccgt gccggcgggc gcgtgttcga
     1501 tataaatatc aaacggtttt ttggttgaaa cggcggcggc gcggacgcgg cttccgtccg
     1561 gcagcgtgca accctcggtc aaatcgcatt gcgccgccac ggcttgcggc tgctgcgcct
     1621 gccaccattg caacaaaacg agcttgacgg cggcaaaggc aatcagcagc agtgcggcaa
     1681 gcagtaattt acggttttta ttcatgattc ttccctattg tttcatccct taccaccacg
     1741 tgcgccccca atccgagcca tagttttcca taacgtgcaa ccgtctgtcc gtttgctgca
     1801 agtacgagcg gcacggatac gccctgccgc agggcttctg ccgcctcttg tgcctgaaca
     1861 tcattgcaca cgcgccacac gacaacatcc gcgccctttt cctgcgcctg ccgccattgt
     1921 tcgcgggtct gcaccatcat ccacacgctg tcggcgtgtt cgggtctgtc ctgccattgc
     1981 gccgcctcca tcaaaacgtt cgcaccgctt ccctctgccg aacccaaagg caggacgcgg
     2041 tacgcgccca tactgttctc cccgtgcaaa cccgttttca ggctgccgta caaacggcgc
     2101 ggaacggaca gcgaacgcaa aagcgcgccg ttggcgggca gcatgggggc aacggtaaaa
     2161 tcacccgcct tctgccaaga ccattcctgc ccttcgcggg attgcggttc gcccgtccat
     2221 tggccggggt tgacccatag gaatttcagg cagacgcggg cgtgttcgta ggaatggatt
     2281 ttggtcagcc aaggcgtggc ggcaaggatg cggatgccga gttcttcttc aaactcgcgt
     2341 tgcaaggctt ggaagtcggt ttcgcccgct tcgaccttgc cgccggcaaa ttcccaatat
     2401 ccggcatagg gtttgccttc ggggcgcgag ctgagcaggt agttgccgtc tgaatcgagc
     2461 aggatgccgg caacgacgcg gataaggggt cgggtgtctt gaatcatggc gggtacgcgg
     2521 ttggtcggtc ggagtgcaaa tgagggacga gcaatgccgt caacgggttt gccgcatttc
     2581 agacggcatt atttttcggc aatgacaaac gccaatacgg tgtcttcttc gtcgctcatg
     2641 ctgaggctga cgcggctgat gccttgttcc tccagccatt tggacagggc ggggccgtag
     2701 aaaaattcgg gcttgcccaa tgcgtcatgc ccgatgccga tgttgcggaa ggaaaccgcg
     2761 ccgcgtatgc ccgtaccgac ggctttggcg aaggcttctt tggcggcaaa gcgtttggcg
     2821 aggtagttga cgggtttgcc cgcttgcgga aattcaagca gctcttctgg agtgaggatg
     2881 cgcccggcaa acgcctgtcc gaattttttg tttaagcgga tgatgcgctt gagggaaaca
     2941 atgtctgtgc cgatgccgta aatcatattt gcgctccttc gcccttggtt gcgggtagtg
     3001 atgaatgtga tgatgaatgc cgtcaatttc ttggggcgtt aaaatggcgt gaatatccac
     3061 ttttggggtt ttgtcggtaa caattttcac tgtaaccttt tgcatttgaa attctaaaac
     3121 atcgtgccaa ttatttaata aggagttaag gcatttaata atgaattaaa gtttgattaa
     3181 acccgaatgc ccgattttca gagccaatac caataaaaca ataaaaatta acattttaaa
     3241 aaaatttggg ctaatcttag tccttaaccg aattccaaca tacaatccaa taacagacaa
     3301 tacggacagt aaaaatatta aaccgtattc actcttattt aataaccaat actggtctct
     3361 tagcatatat atttgaacaa ttttcgccaa aagatagcat agattgcttg attttacgat
     3421 acgattttta ttttctgttt cgctaagcaa aaatattaac aatatgggag acatggcatt
     3481 ggttgaaccg ccgatgatgc ctgccaaaaa cccaaaaaga atcatattct tattattggc
     3541 aactacttga atattttttg cttttgcaca tacatttaaa ataccattaa cagaataata
     3601 caatgtaatg attgccatca gtaaaagcag ccacgacact ggaagtatca aaagcaactt
     3661 cacccccaaa atgctgccaa cgacgctgcc gatagcaagc aatttatagg tttttaaata
     3721 ataaacaatc tcttgccaaa aacctttttt gttattgctg catagaacca acaagctcat
     3781 taacaggctt ggtaatgcca ccaaggcaac aaccttagac aatggcatgt taaaagccaa
     3841 tgcggttgta ccgagcatcg gaaatcccat gcctgtaatt ccgtgcagta ttgcggcagc
     3901 aacaaaaacg atagattgca ttatttcttg cataaccgtc ctttatacag taattcttgc
     3961 tctaatataa tgcctgtttt tatccgtata atcagttccg gcatgcaagc cattgacatt
     4021 gtcaataata atcaaaccat catcttgtgc agaaaattct tgaatatcac tcgcattttt
     4081 tataatcttt acaagataat caagtgcaag taccatttcc tttgatactt tatttctatt
     4141 ttcttcaata cctttataga tacaatcact tctaaatcta atcatttgag tattgaccga
     4201 taagatatta ccccatctca caccttgttt tttatcaaat gatgctggtg ctttaaatgg
     4261 atataaattg cccgttaatg tttttaagtg ttttttaccg gtttctgttt tagataactg
     4321 attgacaata tctgatgaac ttaaaaatag ggaattacct ccatcattgg ctgattttac
     4381 tacatacata accaaataac tttccgggtt ttcactaaaa gatgaatcac tatgaagtgg
     4441 acattctcca acatcttccg agaaagttat atcattattt atagtggatt aacaaaaacc
     4501 agtacggcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc tgaagcacca
     4561 agtgaatcgg ttccgtacta tctgtactgt ctgcagcttc gtcgccttgt cctgattttt
     4621 gttaatccac tatattacat aatcatcgcc tcaatattta atatcccaaa atatagattt
     4681 aaaaccatcg cctttgtggt ttgtcagcat tcctaattga gatagaaagt caaataattt
     4741 cttgtttctc cggctgccat caagatggtt tagattaaga cctcgaacaa tacaataccc
     4801 aatactctgt actgtattgt gaatttcttc tatcaaacta ttattgtttg cagataaaat
     4861 atacttttga ttcatatagt gaattaaatt taaaccagta cggcgttggc ttgccttgcc
     4921 gtactatttg tactgtctgc ggcttcgccg ccttgtcctg atttaaattt aatccactat
     4981 aacatatccc tgtcgtgaaa tgctgtctga aagggtttgc ctgcccttac ggcagcagcc
     5041 ttgccctgaa catcgcctcc ttcatttggc gcacggcttc gggcagtccg aggaagaggg
     5101 cttgggcaat cagcgaatgc ccgatgttca gttcgcggat ggcgaggatt tgggcgatgg
     5161 gggtaacgtt gtgtatggtc agtccgtgtc cggcgttgac gaccaagccc aaatcgccgg
     5221 caaaatgcgc gccgttttgc agacgctcga attgcttgag ttgttcggcg tggctgcgcg
     5281 cgtcggcata cgcgcctgtg tgcagctcga caacgggcgc gccgacatca taggcggctt
     5341 ggatttgcct gtcgtcggca tcgataaaca aagacacgcg tatgcccgcg tcggtcagga
     5401 ttttggtgaa ttcggcgatt ttttcctgtt gcgccaatac gtccaaaccg ccttcggtcg
     5461 tgatttcctg ccgtttttcc ggcacgatgc acacgtcttc cggcatcact ttaagcgcgt
     5521 tttcgagcat ttcttccgtc aacgccattt caaggttcag gcgcgtgcgg atggcgtttt
     5581 tgacagcaaa cacgtccgcg tctttgatgt ggcggcggtc ttcgcgaagg tgcatggtaa
     5641 tcaggtccgc accgtgcgtt tcggcaacca gtgccgcctc cacggggctg ggataagtcg
     5701 tgccgcgcgc gttgcggacg gtggcgatgt ggtcgatgtt gacacctaaa agcataatct
     5761 ttccttttat ttctgccttc agacggcatt tgaagccgtg ccgtccgaag tcgggacggt
     5821 ttcccgggcg gtttctttgc ggtcaaactg ccgtatctgt tccaacacct gccgcgattt
     5881 cagcccttcg ggcaacaggt ggcggataaa aagccgtgta attttcaatg cctgttgcag
     5941 gctttcggcg gtgcggaaac tgccttcgcg caaatcgatc aggctttgcc cttcgacaat
     6001 tgcgccggct tcgtgcgaaa gcgcgtctgc gtcctcgcat acgggcatga ccgcctcttc
     6061 gggcatcagc cggtaggttt tgtccgccaa aatatcgccg cccgtgccgt cggtgtgcaa
     6121 atcgggggca acgcccaagg cattgagcag cttccactcg aagcggcgca aatcggcaat
     6181 gtggttggct tcacgacaca ccgcctccat cacttttgcc aacgcgtcgt acagttcggg
     6241 catcggatct tcgcgcgcgg tcagtttcaa caccagttcg ttcacataca gaccgctgaa
     6301 caacgccctg ccctgcggct gccgccatcc gccgacccat tcggcacggt gaagggtttt
     6361 gagttcctga ctgccgtacc acgacgcgct gacgggcacg aaggaaacca gcacgccgcg
     6421 caattcgctc tgccgcttgc gcgcgcttct tgccaaaagt gccacacgcc cgtaacggcg
     6481 gctgaaggct tcgacccgca ggctgctttc gcgccatggc gaggaagcca acaggaagac
     6541 gggttcgtgg ttgactcggt gttcggacat catgcctgaa atgccgtctg aacgctgctg
     6601 ccgctacaaa acggcggtcg ggtacgaacc gatgactttg acgaacgaag cgcgttcgcc
     6661 caagcgttcc aatgccgtct gaatctgcgc gtcctggcgg tgtccttcga tgtcgatgaa
     6721 gaacaggtat tcccacaaaa cggatttgct cggacggctc tcaaacttgg tcatggaaat
     6781 acccgactcg gtcagcggtt gcagcagcga ggcaaccgca ccggcgcggt tgggcgcgga
     6841 aacggccagc gaagtcttgt cgctgctgct tgcaccggtt tcgtgatgtc ccatcaccaa
     6901 gaagcgcgtg gtgttgttcg gttcgtcttc gatgcactcg gcaaccatat cgagtccgta
     6961 gatttccgcc gccgtgcgtc cggcgatggc ggcaaccgta ccgtcgtccg attcggcaac
     7021 cagtcttgcg gcttcggcat tgctggacac ggcaatccgt tcggcgttgg gcaagtgtct
     7081 gcccaaccag tcgttgcact gcgccaacgc ctgcgcgtgg gaaaagactt tggcaatgcc
     7141 ttcggtgctg ccgttgtttt tacgcaaaag gttgtggtgg atgcgcaaaa cgacttcgcc
     7201 gcacgcctgc aacgcggtaa cggcaagcaa atccaacgtg cgcccgaccg agccttcggt
     7261 cgagttttcc acgggcgcga ccagataatc cgcctgacgg gtttcaacct gcttgaagca
     7321 gttgtctatg gtcggacacg ccatggtgtg cgcggcgtgt ccgaaatgct tgatcgccgc
     7381 ctgctgggta aacgtgccct gcggccccag ataggcgatg gtcagcgggc gttctacggc
     7441 gaggcactcg ctcatcactt cccgaaacag gcgtgctacc gattcgtcgg gcagcgggcc
     7501 tttgttcaaa tcctgaatgc ggcgcaacac ggcgacttcg cgttcgggac ggtacactgc
     7561 gcccgtgcct ttcagttcgc cgatggcgtg tgcgtgttgc gcgcgttcgt tgagcaggcg
     7621 caggatttcc gcatcaatgg tgtcgatggc gttgcggtgg ggaaggagga gttcgtcgat
     7681 agtttgggac ataatttcgc ctgaacagta atttatcgtg cattctaaca tcaaatgccg
     7741 tctgaaacgg tttcagacgg cattttccgt taaaggtatt cgctttgccc gttttctttc
     7801 cacgcacgat gcgagcagag ccacagaagc gcaatgccgc aagaggtgga cgcggtcatc
     7861 gttgccgcca tcacggttgc cgaaccgtcg tgcaagaagg tcgccgccat acccaccccc
     7921 gcgccgatta aagattggaa tacacccaat acggcgtttg cgctgccgcc ctcttctttg
     7981 aaataggaca taaaacacgc ctgcgtgttt gcaccgacca agccctgcgt accgacggaa
     8041 aacatcacgc acgcgaccag cagccaaaac gggggcaacc cgaaaaacag cacggcggcg
     8101 agttgggaca ggttggcggc aaactggacg acaatccccc atagcaggat gctttgcgga
     8161 tgcacgccgg ttttgagccg ccacgcggta acgcggttga aaaacatcat cgtgatgatg
     8221 tttagtgcaa acgcccaagc gtattgatga ggcgtaacac ggtaaagctg ctggtacacg
     8281 aaggaagatt cggtcagaaa ggcgaacatc gaaccgaagc tgaatgcctg aaaaaacaga
     8341 taacccatcg cggcacgggt tttcaatacg cgtttgaacc gcccagccac cagcccgaac
     8401 acatcccgtc cgattttgcc gccgacggca ggcttgggca ggaaatactg caccaaaccg
     8461 agcagcacca gcgaatacgc cgccagaaaa acaaaaatcg cctgccagcc acccaagccc
     8521 tgcaacagtg cgccgaccat gggtgcgacc agcggcacaa ccatcaaaat gatgccgata
     8581 agggcaaaca tctgggcggc tttgcgtccg gaataataat cgcgcaccat tgcgccgacg
     8641 atgaccacag tcatgcccgc accgaatgcc tgcacaacgc gcaggttgag gagctgttcg
     8701 gcactcgaag caaatacgat ggcggcaacg gcaaggcagt agacggccag tccggtcagc
     8761 gcgacgggtt tgcgcccttt gatgtcggac accgaaccgc cgaccacctg tccgaacgcc
     8821 gtgccgaaca taaacaaact caggctctgt tcgatgcggt ggacatccgc gttcagcgac
     8881 tgcgccattt cgggaatcgc gggcaggtag gcatcgatgg aaaacggcat cagcgtaacc
     8941 agcatcgcca tcaaaaccgc catcagtttt tcgctcattt caggataatg ggcagaaggc
     9001 ataaattctc gtccgattcg caaacaaacc gcccggcggc ggaaaacaca atacgcggaa
     9061 acggaaaatt ttaacacacc ggcacaaaca ggcggcaaca aaagccccgg caaaaccgga
     9121 cgaaccggaa cgatatagtg gattaacaaa aaccagtaca gcgttgcctt gccttagctc
     9181 aaagagaacg attctctaag gtgcttaagc accaagtgaa tcggttccgt actatctgta
     9241 ctgtctgcgg cttcgtcgtc ttgtcctgat ttttgttaat ccactataaa atcccgtcat
     9301 tcccgcgcag gcggaaatcc ggacatccaa cgctgcagca atttgtcgga aataaccgaa
     9361 actcaaaaaa ccggatgccc gcctgcgcgg gaatgacgac gcaaagatgg tcgttatttc
     9421 ggataaatat ccgtcttttc gttccggata cccgctttcg caggaatgga gatggaaaga
     9481 tttcagccta ttcggcaatc atcaaatacc gcttcctaac tttatttttg atgcgacagc
     9541 cggccggcaa ccaaggtggc ggcgatgacg acaaacacgc ccaaggcgga cacgggcgac
     9601 aggtgttcgc ccaaaatcaa aaccgccagc agcacgccga cgacgggttc gagcgaaatc
     9661 aacagtcccg aaacgttggc aggaacacgg ctcatcccct tgttccacag ccaataggcg
     9721 taccagccgc accccaaacc caaatacagc agcgacaata ccattccgac gctccagtcc
     9781 acggtataac tttgcgccaa agcaagcgaa aacggcaggc acatcaacga tgcggcggca
     9841 atggaaacag atgtgaatgc cggtgcgccg atgcgtgcaa tcagcctttg cgtcggacgc
     9901 atagcggcac aaaagcccgc gcccgccaac aacaccagca ggcagccgaa ccagccgact
     9961 tcgccgccct cttccgcacc gcccgccatc agcagcgcga caccggcaaa tgccgccgcg
    10021 ccgcatatcc agtggtaggc acgcgctttg tcgttgaaga aaaagtgtcc gacaaacacc
    10081 atcagcagcg gctcgagtcc gacaatgacc gatgcgctgg cggcggaagt gtatttcaac
    10141 ccgacaaact gaagcagcag ggtcagcaca tagttgacga acgacacaat cagcaacggc
    10201 ttccattcct cacgcggaat cttgccgaca tgacggcggc aggcgggcag tgcaggcagc
    10261 gcagcaatca gcaggcgcac gccgaccatc aatgcgggat cgatgccgcc atagacatat
    10321 ttggcggcaa taaacgagct gctccagata atcagggcaa ggatttggta aaacatagtg
    10381 agtgataaag tcagatggtc atacaaaagt atagcggatt aacaaaaatc aggacaaggc
    10441 gacgaagccg caggcagtac aaatcgtacg gaaccgattc acttggtgct tcagcacctt
    10501 agagaatcgt tctctttgag ctaaggcgag gcaacgccgt actggttttt gttaatccgc
    10561 tataacagca accttgtcgc cgtcattccc gcaaaagcgg gaatccagga cgtaaaatct
    10621 caagaaacct tttttcccga taagtttccg caccgac
//
LOCUS       DAXHTY010000063        10575 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXHTY010000063 DAXHTY010000000
VERSION     DAXHTY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.063"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(231..1295)
                     /locus_tag="ACFNMK_001424"
     CDS             complement(231..1295)
                     /locus_tag="ACFNMK_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6364679.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRDCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLYALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTDRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
     gene            complement(1296..2054)
                     /locus_tag="ACFNMK_001425"
     CDS             complement(1296..2054)
                     /locus_tag="ACFNMK_001425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6364680.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAKKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2129..2204)
                     /locus_tag="ACFNMK_001426"
     tRNA            complement(2129..2204)
                     /locus_tag="ACFNMK_001426"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2169..2171),aa:Ile,seq:cat)
     gene            complement(2333..3367)
                     /locus_tag="ACFNMK_001427"
     CDS             complement(2333..3367)
                     /locus_tag="ACFNMK_001427"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF006587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFC6364681.1"
                     /translation="MEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLCVDAAEQALTDA
                     GLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGMSRNFSANTYVRMMPYTAAAN
                     IGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFFPSEVYVFDS
                     LYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAIIYAELVGYGA
                     NSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHHNDSMESRAVAA
                     VFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGFADRQSNPEGKLPPQLWDGQNDPDLP
                     AINLTGSGSF"
     gene            complement(3371..3935)
                     /locus_tag="ACFNMK_001428"
                     /pseudo
     CDS             complement(3371..3935)
                     /locus_tag="ACFNMK_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            3982..4482
                     /locus_tag="ACFNMK_001429"
                     /pseudo
     CDS             3982..4482
                     /locus_tag="ACFNMK_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            4485..4568
                     /locus_tag="ACFNMK_001430"
                     /pseudo
     CDS             4485..4568
                     /locus_tag="ACFNMK_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            4849..5283
                     /locus_tag="ACFNMK_001431"
     CDS             4849..5283
                     /locus_tag="ACFNMK_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364682.1"
                     /translation="MCGLWLLRALTAKLPQQRYAALFITLFFAAVLVFGRHDSMYWYP
                     VLVNLMMLAVFGGSLFAGQTVIERLARLQQPDLPEKAVRYTRRVTQVWCVFFIANGTL
                     AALLARLGRYDWWAVYTGVIAYVLMGMPFAGEWLYRKLVLKV"
     gene            5290..5805
                     /locus_tag="ACFNMK_001432"
     CDS             5290..5805
                     /locus_tag="ACFNMK_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC6364683.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYESDTFGLHCNVVTQSDNQRPLLQLHQSGGHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            5824..7302
                     /gene="ubiD"
                     /locus_tag="ACFNMK_001433"
     CDS             5824..7302
                     /gene="ubiD"
                     /locus_tag="ACFNMK_001433"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC6364684.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFEHPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVDVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGSVIKKDPAVTAKIDGIWEELGL"
     gene            complement(7366..9480)
                     /locus_tag="ACFNMK_001434"
     CDS             complement(7366..9480)
                     /locus_tag="ACFNMK_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC6364685.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLEAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDTPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            9571..10464
                     /locus_tag="ACFNMK_001435"
     CDS             9571..10464
                     /locus_tag="ACFNMK_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC6364686.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSWENRAEDSRVAAKGVAFGGGETVCIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLNHTKGHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
BASE COUNT         2320 a         2578 c         3040 g         2637 t
ORIGIN      
        1 gtactggttt ttgttagtct actatacatt gtaacaaagc acttgatgcg gcagcacaag
       61 ccggggaaga cgctgcaaaa ctttgccaat aggctgcgta atatcggggg atattgggat
      121 tttcttattt ttaaggatgg attcggggtg tttctttcca atgaacgaat ataatttggc
      181 ggtttgttgc atcaaaggct ttcagacggc atatgccgtc tgaaagcagt ttatattgca
      241 tccaataatt tgtcggcggt atggttgatg gtaaaacggt tggcgacgca ttgatgtcct
      301 gccgttgcca gtgcgttttt cgtttcaggg ttcaagacga ggcgttcgat ttcgtccgcc
      361 aaagattcag gtgtcagcct gtccagcaga atccccgatt gatgatgttc gataatttcc
      421 ttttgcgccc ccaaagtatt ggaaatcacc gccgttcggc agtacatcgc ctcgcataat
      481 gacaaaccga atgcctccgg gacgaggctg ggcaaaacca cgacatcgct ttggcggtaa
      541 aacgaagcaa gtttttcggt aaacccttca aaagaaacaa atggttctgc tcctgaagca
      601 gatacgtccc gcttcaggcg gcacatataa tccggatgtc cgtcccctgc caatttgagc
      661 ctgatttgag gatatttccg atgcagtatc acacaggctt caatcaggtt ttccaatcct
      721 ttttctggac tgatcctgcc ggcgtaggcg acggtaaaaa aacggctgtc gggtttttct
      781 tgagagggag ggaagcgatc ggtatcgata ccgttatgaa caatccggta tttttcttta
      841 aagggattgt cggcggtttg cacatcgtaa accagacggg aaacgcaaat aaagcggtcg
      901 gtgtttttct gtatcaggcg gtgataaaaa tcagttttgt tggcgacaac attatgtttg
      961 acaaatatca ggcgcacccc ggtcagtttt ttcaaaagta tggataaggc ggcaattttg
     1021 ccggtgtgta tcatcaggtg ggaaatgcgg tttttccgga taaagcgggt aagtgcgtag
     1081 agcgaaaaca gcccgttgag cgtgtgaagg cgcgttgtga aaacggaaga aacttcggaa
     1141 aatcgcctgt gcatcaattc attattttta ttgacggcgg taaacattgt gcagtcccga
     1201 agccccaatg cttttgaaac atcatagaca tattgttcgc ctccacccca aatcttggcc
     1261 gatacgatat ttaatatcct gaattctttt tccatttaaa attttccgcc ggatttgtac
     1321 agataatata gtttgacata tttaatcatc gtgtaatagc tgtggttgac ggacataatc
     1381 caacccattt ttccatcaag aaaccctttg ttcaggatat aaattttgaa aaacccccaa
     1441 atcgggcgga ggataatgtc cctaacgaaa cgcacgggct ttccctgctc tcggtatttt
     1501 ttggctgaaa tggaagtata tttgttgaac ttgttgaaat attgttccca gttgtcgtac
     1561 gtgtaatggt acataaaatg cttcagacgg cgtttggggt agggggtttg tacggtttcg
     1621 tgtactttgc cttgcaccga actgtctttt ttcggcatca gacggcatac gctgtcggga
     1681 cgcatcgcgc cgtgtgtggc gggatggttg gggaaaaggt tgcggcgttc gacaaaatag
     1741 gcggcataat cgccggtttg gacaattttt gagatttcat cagatagttc cggcgtgcag
     1801 cgttcgtctg catcaatcag gaaaacccat tctccgcctg cctgttcgat ggcaaatgtt
     1861 ttttgcgctc cgaaatcccc attcaaatgc cgtctgaaga cttttgcgcc caaaccctcg
     1921 gcaatttcgg cagtattgtc ggtgctgtag tcgtcgataa cgatgacttc tttatcgaaa
     1981 cggcaacttt caatacattc ctgaatgtgg tttgcttcgt ttttggcaac aatcaatacg
     2041 ctgacttttt tcattatcaa ggtttctcaa agcattttgg tttcacaggg cggcaaaaaa
     2101 gcccaaacat acgtccgggc ttgggaattg gtgggtccgg tggggttcga acccacgacc
     2161 aagggattat gagtcccctg ctctaacccc tgagctacag acccgtcaag agtaggcgcg
     2221 tattgtatag catagtggtg ggattgacaa acttatttca tccgatgatt aagacgcagt
     2281 tgcttcctcc gaaggcaaac gacgagctgg cggcaatgtg tttttccgtt gtctagaagc
     2341 tgccgctgcc ggtcaggtta atggcgggca ggtcggggtc gttctgcccg tcccaaagct
     2401 ggggcggaag ttttccttcg ggattgcttt gccggtcggc aaagccccac gcgaacgcgg
     2461 cttcgattgc gcccgccgca cccagcgtgt gtccggtttg cggcttggtg gacgtgcagg
     2521 gcgtattgtt gccgaaaacc gctgcaacgg cacggctttc catactgtcg ttgtggtgcg
     2581 tcccggtgcc gtgcagatta atccagccga tgtcttcggg cgcaagacct gcgtgttgca
     2641 atgccgtctg aaaggcaagg attgcgcctt gcgcgtcggg gcggggcgtg gaaatatggt
     2701 aggcatcgct gttggctccg tagccgacga gttcggcgta aattatcgca ccgcgccgtt
     2761 tggcgtgttc caattcttcc agcacgaaaa tccccgcgcc ttcgccgatg accagcccgt
     2821 cgcggttcgc gtcgtatggg cgcggggttt tttccggttc gccgttgcgg cggctggcgg
     2881 cataaagcga gtcgaaaaca tacacttcgg acgggaaaaa ttcttcgcct ccgcccgcca
     2941 gcatcatatc ggtcagaccg tatttgatgg cttcgtaggc ataacctatg ccttggctgc
     3001 cggacgaaca cgcgctcgat gtcgggatga tgcgtccttt cagtccgaaa aagatgccga
     3061 tattggcggc ggcggtgtac ggcatcatac gcacataggt gttggcactg aagttgcgcg
     3121 acatgccggt cagcaacaat tcgcctacat cgccgatgtc tttggtgctg ccgctggaag
     3181 agccgcaggc aacgcccatc cgtccgtcgg taatgctttc gtccccgagc aaaccggcat
     3241 ccgtcaaagc ctgctccgcc gcatcgacgc acaaatacga cacacgcccc atacttctga
     3301 gctgcttgcg cgtccaatgt ttcggcggcg cgtaatcctc aatcggcgca cccagttgcg
     3361 cttccaattc ggggaaacgt tcgtgccaat ccactatatt ttcgcaagac gcgttttgcc
     3421 tgattccaaa agttctcaat gccgttaatg tggttttgac ggtctgcaaa ttccttggaa
     3481 tggttgatgc ggtaatggat aaaactgctc acgtccaact tgtcgcagct gctcagacta
     3541 tcggtataaa caatactgtc cggcatgatt ttctttttga tgacagggag taacgtttca
     3601 gacttggcat tgtttacgac aacggtatag acccgtccgt tgcgtttcag aatgccgaaa
     3661 acaaccactt ttcctgccgc accgcgacca cgtctgcctt tacgccgtcc gccgaaatcg
     3721 ctttcgtccg gctcgacagg atcctcaaaa acctcatcgg cagccaagac caaatgatgg
     3781 ttgataaccg tgcggatttt acggtagaac agtgctgccg aattgggatg gatacccaaa
     3841 atatcggcgg cagaacgggc ggtaacttcc agtacaaaaa aatggagcag ttctttctgt
     3901 acttttttct ttaatttgca gtgtgttatc ttcatatttc gagggtaaca tatctgctaa
     3961 tctagtaaaa tttaatccac tatagtttat gcctcgcacg acggggaaat ggcgcgcagt
     4021 ttcgatttgt ggttggaact gttgaaatcg caaatcgtgt cgccgacttc gttcggtttg
     4081 tcggtgcaca atgcaacggc ggggtaatgg tcgatattga ggcgggatat gagcgagcag
     4141 acggcattgg ccgtgtgtgc cgacggtgtg gaaacggcat tggcggaagc ggcatcactg
     4201 ttggaagagg gatgcgggtc ggttttggtt ttggcggcgg acgatccgct gccggaagga
     4261 tatgccgtat cggcaacgcg cgcgccgttt gcttatgctt tggcgatggt tctgaccaaa
     4321 gggacgcgtt acagcctgac tttatctgct tcggacgata tgccgtctga agccggtatg
     4381 ctgcccgagg catattggag cggtttggaa tgggtgcgct ttctgctgaa cggcagtcgg
     4441 gaatgccgcc gggtatatcg caatcgtgaa tggctgtggc agcccgcaag ctgcaggctg
     4501 aagatttccg caatgtccgc accgtcaacg acgtggtaca ggcggtattg aagattcagg
     4561 cgggctaatc gggtatttct aattattttt cagacggcat gtttttatcg gatgccgtct
     4621 gaaaacattt gtgcggattc aaaatgaaac ccggtttttt cggacatatt tttctgaccg
     4681 tattaagcat tgcctatccc gctttgtggt atagtggatt aacaaaaatc aggacaaggc
     4741 gacgaagccg cagacagtac ggcaaggcga ggcaacgctg tactggtttt tgttaatcca
     4801 ctatattacg gcagggaggc gggctggttt gtctggctgg cggcggcgat gtgcgggttg
     4861 tggctgctgc gcgccctgac tgccaaactg ccccaacagc gttatgcggc tttgtttatt
     4921 acactgtttt ttgccgcagt attggtgttc gggcggcatg attcgatgta ttggtatccc
     4981 gtcctcgtca atctgatgat gttggcggtg ttcggcggca gcctgtttgc cggacagacg
     5041 gtcatagaaa ggctcgcgcg gctgcaacag cccgacctgc ctgaaaaagc cgtgcgctat
     5101 acccgccggg taacgcaggt ttggtgcgta ttctttattg ccaacgggac gcttgccgct
     5161 ttgttggcac ggttggggcg atatgattgg tgggcggttt ataccggcgt gattgcctat
     5221 gtattgatgg ggatgccgtt tgccggcgaa tggctttacc gcaaactcgt tttgaaagtc
     5281 tgaaacctta tgtacacttg cacttcctgc ggcgaaaaac atgaagatat gcccgcaatc
     5341 ggctttaccg cccccgatcc ctataatcaa ctcagtgaag aagaacgcat tgcttatcaa
     5401 gcagaatgcg attctgattt ctgcatcatc cgctatcccg accaaaccga ccgctttatc
     5461 cgcgcggtgc tgccgattcc gattatcggg catgaggaaa cgctggaata tggcgtgtgg
     5521 gtatccgtca gcgaaaagag ttttaacgac tatcaaagcc gcttttacga caatccggaa
     5581 aacgccgttt atttcggtat gatctgcaac cggctgcccc cttacgaatc cgacaccttc
     5641 ggtttgcatt gcaatgtcgt tacccagtcc gacaaccaac gccctttgct gcaactgcat
     5701 caaagcggcg gacatccgct ggtacgcgac ttttatcacg gcatagaata tgccgaagcg
     5761 caggcaagga taggagcggt atccgaatcg gattgttcag gataaaagga caaaaagata
     5821 aacatgaaat acaaagacct acgcgacttc atcgccatgc tcgagcagca gggcaaactc
     5881 aagcgcatcg cgcatcccgt ttccccgcat ttggaaatga ccgaaatcgc cgaccgcgtg
     5941 ctgcgtgccg aagggccggc gttgctgttt gaacacccga ttaagcccga cggcacgcgc
     6001 tacgattatc ccgtgttggc aaacctgttc ggcacgcccg aacgcgtggc gatgggtatg
     6061 ggggcggaca gcgtgtccaa gctgcgcgaa atcgggcaga cgctggcgta tttgaaagaa
     6121 cccgaaccgc ccaaaggcat caaagatgcg ttttccaaac tgccgctact gaaagacatt
     6181 tggagcatgg cgccgaacgt ggtgaaaaac gcgccgtgtc aggaaatcgt gtgggaaggc
     6241 gaaaacgttg atttgtataa acttccgatt cagcattgct ggccggaaga cgttgcgccg
     6301 ctggtaacat ggggcttgac cgtaacgcgc gggccgcaca aaaaacgcca aaatctcggc
     6361 atttaccgcc aacaactcat cggcaaaaac aagctgatta tgcgctggct gtcgcatcgc
     6421 ggcggcgcgt tggattatca ggagttccgc aaactcaatc ccgatacgcc gtatcccgtc
     6481 gccgtcgtgc tcggctgcga ccctgccacc attttgggcg cggtaacgcc cgttcccgat
     6541 accttgagcg aataccagtt tgccggactg ctgcgcggtt cgcggacgga gctggtgaaa
     6601 tgtatcggca acgatttgca agtgcctgca cgcgcagaaa tcgtgctgga aggtgtgatt
     6661 catccgaacg aaaccgcgtt ggaaggcccg tacggcgacc acacgggcta ttacaacgag
     6721 caggattatt tccctgtgtt tacggtcgaa cgcatcacca tgcgcgaaaa cccgatttac
     6781 cattcgacct acacaggcaa accgcccgat gagcctgccg ttttgggcgt ggcgttgaac
     6841 gaagtgttcg taccgctctt gcaaaagcag tttcccgaaa tcaccgattt ctacctgccg
     6901 cccgaaggct gctcctaccg catggcggtg gtgagcatga aaaaacagta cgccggacac
     6961 gccaaacgcg tgatgatggg ctgctggtcg ttcctgcgcc agtttatgta caccaaattc
     7021 atcatcgtgg tggatgacga tgtggacgtg cgcgactgga aagaagtcat ctgggcggtc
     7081 accacgcgca tggaccctgt gcgcgacact gttttggtag aaaacacgcc catcgattat
     7141 ctcgacttcg ccagccccgt cagcggactc ggcggcaaaa tgggtttgga cgcgaccaac
     7201 aaatggccgg gcgaaaccga ccgcgaatgg gggagcgtca tcaaaaaaga ccctgcggtt
     7261 acggctaaga ttgatgggat ttgggaggaa ttggggttgt aggttttacg gtctgccgtc
     7321 tgaagcgtgg gggcgtttca gacggcattt gtgtacgcag gatgtttacg gttctttagg
     7381 tttgagccac tgccattgtt ccagcagggt gtcttcgagg attttctgtt tctctttgcg
     7441 cgaattgatg ccgccggtca gcctgccgta atcgacggta atggacggtt tgtccaccgt
     7501 accggtgatt ttcaggggaa tcggtttgtt tttcggatgg acggcgttgc ggataaggac
     7561 atcttcagac aattcctgcg tatccagatt ggtatagccg ttgctggtaa catagaggct
     7621 gtcggagaag agttcggtat cgatgtggcg gctgatgccg tctgaaattt cgctgttgag
     7681 cgtgaatcgg tggaagggtg tgctgggtgc ggcattgtcg gcagttttgc ccgaaatgcc
     7741 gtttttcagg atattgtcca tgtcgatacc gtgccatgca ccgttggaaa tatttagcga
     7801 caggctgccc tgaagcgagc ggataagctc ttttcgggtt tcgccgcccg cggtcaggtc
     7861 gatgaccgcg tcgccgttgc cgctgaagct gtggaagccg aacaggtctt gcagcagcgg
     7921 ttggatttgg atgttgcttg cattctgttg caggcggtaa gtggcgggac gggtgttggc
     7981 gatgctgatg ccgccttcgg tatggccgcc gtaaagccct gacttgaaac ggctgagcgc
     8041 gatatggcct ttgtcggcgt ggaggtaggt ttccatatcg tccagttgca ggccgggaag
     8101 ttggactttt ccgattttca ggtgcgcctc gatgtcgccg gacagcttgg cgagggtgtc
     8161 gggaaatatt ttgccgtttt gttgccgcac gtcgtcaaga tagggggtca ggttcaattt
     8221 ttgcagtgcg acggcggctt ccagatgcgg tgtgtcttca tgtgtgtatc tgaatttcgc
     8281 ggcaacggtt tggcggtcga atgtgccgtt taattcggca ttccaatttt gcagattcgg
     8341 tacggacagc gaaccgtcga gccggctgat gaaacggggt tgcggcaggc ggttgacggt
     8401 atcctgaagg gtcgatacat acaggcgcgg cgcctccagc cctttgtttt ccgtccatac
     8461 gagcggcgaa ttgagggaga agttggtctg gtggcgcggt gttttgaagc tgccggagat
     8521 ttcggcgttg ccgatgttgg cgatgccgga gtgcaggttg gctttgtcga gtttgaacga
     8581 accgtcccat cgggcatatt cgccgccggc ggtaaatgcg ccgttgacgg tttcaatttt
     8641 aatgctgttg ttcctgagtg ccagcgtagg gatttgggcg gtcaggtgga ggttgcggaa
     8701 ggaagtgtct gcacgcaggc cgaggccggc ggcatccgct ccgccggcgt tgaagcggac
     8761 agaagggctg ccggtggtgg aaatggtgat gccgtgtccg tccagcgaag tggaagcttc
     8821 aaaatggaac ggtgagattt cgggcgtgcc gatgccgtct gaaaggaaca gccccctgct
     8881 tttccacggg acggacagct ttccccaaac cagtatgccc gaactttcaa acggctgccc
     8941 cgacgaatcg ggggattgca ggttgaggtt gatttccttc aggataagct gttcctgcag
     9001 gaaattgagg cggacggtgc tgttttcgac gataatgcgg ttgactgagg cttggcgttt
     9061 ttggctgtcg atcaggtctt ggatgttcca aacacctttc ccgtcgcgcg tcagggcaag
     9121 ttccgcactc gaaaccaccc atttttcaat ctgtatctga tccgaccaca ggtttttcca
     9181 gctcaatccg attttggttt cttggacgga aacggcagtc tggtcgccgc cgggttcggt
     9241 aatggtcagg tttttcagga tgacggtcgg ccggggcagg agcctgcgct gaatgtccgc
     9301 atcaaacgag attttccggt gtgtgtgtgc aatgctttgt tggaggcggc tgcggatgtt
     9361 ttcaggcgtg aaggtgcgat atacggaagc gtgcagcccg actgccgcca aaagcagtat
     9421 cgtcagcacg gccaccgcat atttcagacg gtatttgtgg aaaaccgata ataaatccat
     9481 agcccgaaca atctgtaaaa cacgccacag tataacacgg cttgcaaaaa tcctattttg
     9541 ggcaggccgt ttcttgtgtc aggaggaaat atgattattg tcatgagcag gcgtgccgcc
     9601 gaagcggaca ttgccggcgt tgtcgccttt atccgcagca gaggtctgcg agaacacatt
     9661 tctcacggcg acgagcgtac cgtcatcggc gcaatcggcg acgatagggt tttgagcgtc
     9721 agggaggtgc agacgctgcc ggaggtcgag aaagccgtcc gcattttgga tacatggaaa
     9781 accgtcagtt gggaaaatcg ggcggaagac agccgcgttg ccgccaaagg cgtggctttc
     9841 ggcggcgggg aaaccgtctg tatcgccgca gaaccgtcag tatggcgcaa tgcggatgca
     9901 gtgtttctag atccgttttt cacttcggca aacttatacg acacttcaag tgcggatgaa
     9961 gggcgaggac ggtgcagacg gttggcggaa cagaccgcat cggcgcatgg cgcagggaaa
    10021 cccgtattgg tgcgcgtccg caacgtccgc catgtcgaag cggctttgaa tgcgggggcg
    10081 gacatcctgt atttgggcgg cgggctgatg tccgatcttg ccgtattgaa tgaagccgga
    10141 agcctgaaca ttcccttggt gctgtgtaaa gacaagcacc attctgccga agattggttg
    10201 aatgccgcag aatatgtcgt ctcgcgcggc aaccgccacc tgattctggg cgagtcgggc
    10261 gtattgaacc ataccaaagg ccatccctac cgtttggatg tcgaatccat cgtgaaagtc
    10321 cgccaaatca gccatctgcc cgtgattgcg aatattacgg gattatggaa ccgcgatatg
    10381 ccgcaggaaa tcctgtacgg cttggcaaag gcggcaggcg cgtgcggaat cgtcggaacc
    10441 tgttttgaaa agtcgggcgg atagcaggca taccggcaga ttcaaaggcg gagcagacat
    10501 tcggacaaca tgccgtctgc cgcccgaata gcggtgccgt ctgaagcgca caagacggtt
    10561 tcagacggca tataa
//
LOCUS       DAXHTY010000064        10459 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXHTY010000064 DAXHTY010000000
VERSION     DAXHTY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.064"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..110)
                     /locus_tag="ACFNMK_001436"
     CDS             complement(<1..110)
                     /locus_tag="ACFNMK_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
                     /protein_id="HFC6364687.1"
                     /translation="MEDKQGMTKAVAGVMTDAPADGRKPTTASNLPPLSN"
     gene            complement(181..426)
                     /locus_tag="ACFNMK_001437"
     CDS             complement(181..426)
                     /locus_tag="ACFNMK_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364688.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
     gene            complement(427..777)
                     /locus_tag="ACFNMK_001438"
     CDS             complement(427..777)
                     /locus_tag="ACFNMK_001438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364689.1"
                     /translation="MQNGGGKIYQTADNVEGIMLLKVVPERTVSADAKTRDPMWDNAA
                     LQTSEGVNFIARFLGFFSDGEYRYVDVLQPNHSDIIRYSGKDFPLNQILNHIHPARYA
                     VTFENNVDSKLRRH"
     gene            complement(784..1056)
                     /locus_tag="ACFNMK_001439"
     CDS             complement(784..1056)
                     /locus_tag="ACFNMK_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase"
                     /protein_id="HFC6364690.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWDTMLLLLWFLVRFAYKKPRQN
                     PFRQPDRRRYPDAVCLDYSQTILPEQRRNTGTRRVGQKIQRSRSRV"
     gene            1292..2719
                     /locus_tag="ACFNMK_001440"
     CDS             1292..2719
                     /locus_tag="ACFNMK_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFC6364691.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            2788..4191
                     /locus_tag="ACFNMK_001441"
     CDS             2788..4191
                     /locus_tag="ACFNMK_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFC6364692.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(4355..5142)
                     /locus_tag="ACFNMK_001442"
     CDS             complement(join(4355..4887,4887..5142))
                     /locus_tag="ACFNMK_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC6364693.1"
                     /translation="MSQTAATFNLSRNTLYLWIRLKKQTGSLKHQVTGLNAVKSDRQK
                     PAQYVGQHPDAYLHEIAKHFDCTAATVCYALKQMGITRKKRPTTYKEQDPAKVTHYLT
                     QPAEFSDYQRVYLDETGFDRYLFRPYTRSLKGQIVKAQISGKRYQRLSLVSAQVGNRL
                     IAPMVYQNTMIGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHKVL
                     PLAPYLPELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
     gene            complement(5435..7648)
                     /locus_tag="ACFNMK_001443"
     CDS             complement(5435..7648)
                     /locus_tag="ACFNMK_001443"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6364694.1"
                     /translation="MTAISPIQDTQSATPQELREWFDSYCAALPDNDKKLVLAARSLA
                     QEHYPADAATPYGEPLSDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNVPDNPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
     gene            7791..8432
                     /locus_tag="ACFNMK_001444"
     CDS             7791..8432
                     /locus_tag="ACFNMK_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC6364695.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREGRLKDEVRPEIQAWLDKVDGYNNKLVQPRV
                     IKIGLPEFATAEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFLVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
     gene            8769..9275
                     /locus_tag="ACFNMK_001445"
     CDS             8769..9275
                     /locus_tag="ACFNMK_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364696.1"
                     /translation="MDFFDFFAQGKYKKIATEQGLDRKYFIFHYIVCNLSAISSFCLM
                     KVIHSYTILCLLIMIIGIAFGFILRNRVLSIVKNKQNFLSDMFPLEMPFFSRVYKIPI
                     LARFLNRGMKWLYLQNFFCLVMICFSISLILNEERGWMPYFICYWFGLCYPANTYYVF
                     LSRDYKHI"
     gene            9411..10211
                     /locus_tag="ACFNMK_001446"
     CDS             9411..10211
                     /locus_tag="ACFNMK_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFC6364697.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
BASE COUNT         2560 a         2316 c         2734 g         2849 t
ORIGIN      
        1 ctgttagata aggggggaag atttgaagcg gttgtcggct tcctgccgtc cgctggcgcg
       61 tccgtcatca cgccggcaac cgcctttgtc atcccttgct tatcttccat ggtgcgaatc
      121 ctcaaaaacg ggcaaaaaaa agccctgtta cttgtagaaa gtaaaggacg ttaatttttg
      181 ttaatcgtcc cttcttaggg acgcaatata taagggacgc aatatataag gttttaaaac
      241 gctaagaaca aaatccgata acggctctga agaagtaagt ctttctttat tgcacaagat
      301 agcaaacttc cacggcattc tcgccccacc cgtgccgtcc aagccttttt caaagacata
      361 gatggttttc ttggcaatca cttcgtcagt ttggcggtcg ataatccgta tggtcgcacc
      421 tgccactcag tgcctgcgca gcttggaatc gacattgttt tcgaacgtta ccgcataacg
      481 ggcggggtgt atatggttaa gtatttgatt tagcggaaaa tctttacctg aataccgaat
      541 aatatcggaa tggttgggtt gcaggacatc cacatagcgg tattccccat cgctaaaaaa
      601 tcctaggaaa cgagcaataa aatttacgcc ttcgctggtc tgtaaagccg cattgtccca
      661 catcgggtct ctggtttttg catctgccga aacggtacgc tcaggtacta ccttcaacag
      721 cataatccct tccacattgt ccgccgtctg gtaaatcttt cccccgccgt tttgcattgt
      781 tcgttaaaca cggcttcggc ttctttgtat tttctgtccc actcttcttg tgcctgtatt
      841 tcttctttgc tcgggcagaa ttgtttggga ataatccaaa caaacagcat caggatagcg
      901 gcggcggtca ggctgcctga aaggattttg ccggggtttt ttgtaggcaa agcggacgag
      961 aaaccaaagc aacagcagca tggtgtccca atagccgatt gagaatagga tggccaaacc
     1021 ttctaggaaa tggcgtaaat cgtttgtggt aaacatgggt agttcctgtg gttaaatgtg
     1081 caggctgctt tttgccgaac cttgccgcat ctcaaaagta gcctgcgctt cagcgttgcg
     1141 ttacgcagta aaataatgaa tatttgtaac gacttgggta ttttttgtca atattcccgc
     1201 ctttccctta acagctgccg cgctttccgt taaaattcct ttacatattt atattgtttc
     1261 ctgtttctat attgccaagg ttatacccgt tatgtttttc tccgccctga aatcctttct
     1321 ttctcgatac attactgtat ggcgcaatgt ttgggcggtg cgcgaccagt tgaaaccgcc
     1381 caaacgcacg gcggaagaac aggcgttttt gcccgcgcat ttggaactga ccgatacgcc
     1441 ggtctctgcc gctccgaaat gggcggcgcg ttttattatg gcgtttgcgc ttttggcttt
     1501 gttgtggtcc tggttcggca aaatcgatat tgtggcggcg gcttcgggca aaacggtgtc
     1561 gggcgggcgc agcaaaacca tccagccgct ggaaacggcg gtggttaagg cggtacatgt
     1621 gcgcgacggg cagcatgtga aacagggaga aacgctggcg gaactggagg ctgtgggaac
     1681 agacagcgat gtggtgcagt cggagcaggc tttgcaggct gcccaattgt ccaaactgcg
     1741 ttatgaagcg gtattggcgg cattggaaag ccgtaccgtg ccgcatatcg atatggcgca
     1801 agcacggtct ttaggtctct ccgatgccga tgtgcaatcg gcgcaggtgt tggcgcagca
     1861 ccagtatcag gcatgggcgg cgcaggatgc gcaattgcag tcggctttgc gcggccatca
     1921 ggcggaattg cagtcggcca aggcgcagga gcagaagctg gtttcggtgg gggcgatcga
     1981 gcagcagaaa acagcagact accgccgttt gcgggccgac aattttattt cggaacatgc
     2041 gtttttggag cagcagagca aatcggtcag caattggaac gatttggaaa gtacgcgcgg
     2101 tcagatgagg cagattcagg cggccattgc acaggcggag cagaatcggg tgctgaatac
     2161 gcagaacctg aaacgcgata cgctggatgc gctgcgccag gcaaacgaac agattgacca
     2221 ataccgcggc caaacggata aggcaaagca gcggcagcag ctgatgacaa tacagtcgcc
     2281 tgcggacggc acggtgcagg aattggccac ctatacggtg ggcggtgtgg tgcaggctgc
     2341 ccaaaaaatg atggtgattg cgcccgatga cgacaaaatg gacgtggaag ttttggtatt
     2401 gaacaaagac atcggttttg tggaacaggg acaggatgcg gtggtgaaga ttgagagctt
     2461 tccctatacg cgctacggtt atctgacggg caaggtgaaa agtgtcagcc atgatgcggt
     2521 aagccacgaa cagttgggct tggtttatac ggcggtggtg tcgctggaca aacatacctt
     2581 gaatattgac ggcaaagcag tgaatctgac ggcgggcatg aatgtcacgg cggagattaa
     2641 aacgggtaaa cggcgggtgc tggattatct gttaagcccg ctgcaaacca aattggacga
     2701 aagctttagg gagcgatagg cggatccgta ctgggcattt gttatccgcc ggttcggaca
     2761 tgcagactgc ctgaaaccat tgcccggatg acattgctca atctaatgat aatgcaagat
     2821 tacggtattt ccgtttgcct gacactgacg ccctatttgc aacatgaact attttcggct
     2881 atgaaatcct atttttccaa atatatccta cccgtttcac tttttacctt gccactatcc
     2941 ctttccccat ccgtttcggc ttttacgctg cctgaagcat ggcgggcggc gcagcaacat
     3001 tcggctgatt ttcaagcgtc ccattaccag cgtgatgcag tgcgcgcacg gcaacaacaa
     3061 gccaaggccg cattccttcc ccatgtatcc gccaatgcca gctaccagcg ccagccgcca
     3121 tcgatttctt ccacccgcga aacacaggga tggagcgtgc aggtgggaca aaccttattt
     3181 gacgctgcca aatttgcaca ataccgccaa agcaggttcg atacgcaggc tgcagaacag
     3241 cgtttcgatg cggcacgcga agaattgctg ttgaaagttg ccgaaagtta tttcaacgtt
     3301 ttactcagcc gagacaccgt tgccgcccat gcggcggaaa aagaggctta tgcccagcag
     3361 gtaaggcagg cgcaggcttt attcaataaa ggtgctgcca ccgcgctgga tattcacgaa
     3421 gccaaagccg gttacgacaa tgccctggcc caagaaatcg ccgtattggc tgagaaacaa
     3481 acctatgaaa accagttgaa cgactacacc gacctggata gcaaacaaat cgaggccata
     3541 gataccgcca acctgttggc acgctatctg cccaagctgg aacgttacag tctggatgaa
     3601 tggcagcgca ttgccttatc caacaatcat gaataccgga tgcagcagct tgccctgcaa
     3661 agcagcggac aggcgcttcg ggcagcacag aacagccgct atcccaccgt ttctgcccat
     3721 gtcggctatc agaataacct ctacacttca tctgcgcaga ataatgacta ccactatcgg
     3781 ggcaaaggga tgagcgtcgg cgtacagttg aatttgccgc tttataccgg cggagaattg
     3841 tcgggcaaaa tccatgaagc cgaagcgcaa tacggggccg ccgaagcaca gctgaccgca
     3901 accgagcggc acatcaaact cgccgtacgc caggcttata ccgaaagcgg tgcggcgcgt
     3961 taccaaatca tggcgcaaga acgggttttg gaaagcagcc gtttgaaact gaaatcgacc
     4021 gaaaccggcc aacaatacgg catccgcaac cggctggaag taatacgggc gcggcaggaa
     4081 gtcgcccaag cagaacagaa actggctcaa gcacggtata aattcatgct ggcttatttg
     4141 cgcttggtga aagagagcgg gttagggttg gaaacggtat ttgcggaata aagcaggctg
     4201 aaacggttat gaaattccca aagcagcctg caccccgttt cgaaagtgca ggctgctttg
     4261 ggattgatcc gatactttca cattctcatt gcctttaaat aaagcacagt taaaaacaca
     4321 tttaaattaa gtgcgaatta attcaaatgt atattcaatt aaaatcaaaa taggacagta
     4381 gtgcatcgtc aaatcgggcg taatcagaca aaacggttct cagataccgc ttaatattcg
     4441 cccacacttt ctcaatcggg ttgagctcag gtaaataagg tgcaagaggc aataccttat
     4501 gtcccaattt ttccgccatt tcccgtaaga cacccatacg gtgaaatcgc gcattatcta
     4561 aaataatcac cgatttttga gtcaatgcgg gcagtaggca ttgctgaaac cacgcttcaa
     4621 aaaagactcc gatcatcgta ttttgataaa ccatcggagc aatcagccgg ttgccgactt
     4681 gtgcggacac caaagataag cgttggtatc tttttccact tatctgcgct tttactattt
     4741 gccctttcag gctgcgggta tagggacgga acaggtagcg gtcaaatcct gtttcatcca
     4801 aataaacacg ttggtagtcg gaaaattcgg ccggctgtgt caaataatgc gttactttgg
     4861 ccgggtcttg ttctttgtaa gtggtggtct ttttttgcgc gttatcccca tctgtttgag
     4921 tgcatagcaa acggtggctg ccgtacaatc aaaatgtttg gcgatttcat gcagataggc
     4981 atccgggtgt tgcccaacat attgagccgg tttttgccta tccgatttga cggcatttag
     5041 accggtaact tgatgtttta ggctgcctgt ttgtttttta aggcgaatcc acaggtaaag
     5101 cgtgtttctt gacaagttaa acgttgctgc ggtttggctg atgtttttgc attgttcgta
     5161 atatcgtgga ttaaatttaa accagtacgg tgttgcctcg ccttgccgta ctatttgtac
     5221 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatagtt taaagctttg
     5281 tttcttaagt ccgcagagta tgccatggtt agaccttcaa agttgagtat tgtactattt
     5341 tgtttttaat tgactatgca acaaaaaata gcaaaccccg gcaatcaaaa tgccgtctga
     5401 agcgttattc ggctttcaga cggcattttt gtatttaaag ccgggtaacg ctcaatacgc
     5461 ctttgacgtc gccgaggctg gcgaggacgc gcgggaggtc gttgacctgt ttgacttcga
     5521 gcgtgaaccg catactggct tccaagtcgc gggactgggt ttgcacggcg gtaacgttga
     5581 gtttgtggcg ggcgagcgcg tcggatacgt cgcgcaaaag cccggagcgg tcttgggcgc
     5641 ggatttcgat atcgacggcg aacacttgtc cttcctgcaa cgccgcccaa cttgcgtcca
     5701 gtactttttc gggcgcgtgt tcggcgaggt gtcggaaaga ggggcaggtt ttgcggtgta
     5761 ccgaaatgcc gcgatcgcgg gtaacgaagc cgacaatgtc gtcgggcggc gcgggtttgc
     5821 agcatttggc aagcgtggtc atcagaccgt cttcgccgtc gatgagcacg ccgtttttgc
     5881 cgcctttttt gattttggac tgtttgacga tggtggtttc gctgacgggt acgggcggcg
     5941 gttcgttcag cgtgccgcag gctttttgga tggcgcggtt ggaaatttcg ccttgtccga
     6001 cggcggtgta gaggtcttct ggctttttgt agccgagatt ttcggcaagc tcttgcaggt
     6061 tgggtttggg cgtgagtttg gcaagctgtt tgtcgagttg gacgcggcct tcttcgcgca
     6121 cggtgtcggc gttttgctgg cggatgtagg cgcggatttt gccgattgcc ttgttggatt
     6181 tgacccagcc ttcgtaaagc cagttgacgg aaggatgccc ttctttggcg gtaatgattt
     6241 cgacacgctg tccgttttcg agcggggtgg acagcggcac aatctgccct tcgactttcg
     6301 caccgcggca acggtcgccg atgctgctgt gtaaggcgta ggcgaagtcg atgggggttg
     6361 cgcccgtggg cagggagagg actttgccgt gcggggtcag gacgtaaatc gtgtcgttga
     6421 aaagctcggt tttgaaggcg gcggcgaggt cttccttgcc gctttccgcc atgttttcac
     6481 gccagtccag gagttggcgc aaccaggcga ttttttgttc gtaggctgaa tcgcctttgc
     6541 cgccctcttt ataacgccag tgggcggcga caccgaattc gttgaattgg tgcatatcga
     6601 aggtgcggat ttgtacttcc acgcctttgt cttccgggcc gacaatgacg gtgtgcaaac
     6661 ttttatagcc gttgcctttg gggttggcga tatagtcgtc gaactcgccg gggatgggct
     6721 gccagagact gtggacgata cccagtgtgg tgtaacactc ggggacggta tcaaccagaa
     6781 tccgcacggc gcggatgtcg aacagaccgt cgaagctgag ttttttcttc accatttttt
     6841 tgtaaatgga gtagatgtgt ttcggacggc cggcgacttc aaagtggata ttgtattttt
     6901 tgagttccgt acgcaggata ttgaggaagt tttcgatgta ttcgaggcgt tcggtacgtt
     6961 tttcgtccaa aagcagcgcg atttcgcggt atttttcggg ttcttgatgg cggaagccga
     7021 ggtcttcgag ctgccatttg agctgccaca cgcccaagcg gttggcgagc ggggcgaaga
     7081 tgtcgagggt ttctttggcg acggcgcgtt tttcggggtt gtcggggacg ttgcttaaaa
     7141 attgcagggt gcgcgtacgc atcgccagtt tgattaacac gacgcggatg tcggtaacca
     7201 tcgccagcag cattttccgc atagtttcgg cctgctgggc gcgttcttcc ggcgtggcga
     7261 ggctgtccac gcgggcgaag tgggtaagtt tctgcacttc gtccacacct ttgaccagct
     7321 cggcgacggt gctgttgcag cgttcggaaa ccaagagatt ccagtcgggg acgtagcgtc
     7381 cgatgtcggc aagcagggtg gcggcgacgg catcgggcag caggtcgagt tcatggacca
     7441 tttgcgccgc accgaggaag tggtcggaca acggctcgcc atacggcgtg gcggcatcgg
     7501 cggggtaatg ttcctgcgcc agtgaacggg cggctaagac aagtttttta tcgttgtccg
     7561 gcagcgcggc gcagtagctg tcgaaccatt cgcgcaattc ttgcggggtc gcgctttgcg
     7621 tgtcttgaat cgggctaatg gcggtcatga ttgggtcttc cgttccgata aaaacgagtt
     7681 tattgtaaca gcttatttat ttacggtgca taggcgggaa gataaacttg tttcgggcgg
     7741 cgcggggtgt taaggtagcc ggtaccgaca ggttgacgag gaatattttg atgaaactgt
     7801 atatttacga ccattgcccg ttttgcgttc gtgcgcggat ggctgccggg ctgtttggcg
     7861 cggacgttga agaagccgtg ctggcaaacg acgacgaggc aacgccgatc ggtatgattg
     7921 gcgcgaaaca agtgcccgtc ctgcaaaaag aagacggttc gtttatgggc gagagtttgg
     7981 acattgtgcg gcatttcgac cgcgaaggcc gtctgaaaga cgaagtccgc cccgagatac
     8041 aggcgtggtt ggacaaggtg gacggataca acaacaaact ggtgcagccg cgcgtaatca
     8101 aaatcgggct gcccgagttc gctactgccg aagcggttaa atatttcacg gacaaaaaag
     8161 agaaaagcat cggcagcttt tccgccaatt tgaataaaac cgcccaatac ctcgaacgga
     8221 taaacgcaga tttgcaagag ttggaaaatt tgatggacgg tgcttcagac ggcatcaacg
     8281 gcggaatcgg tatggaggat attttggttt tcctcgtttt gaggaatctg accgtggtgc
     8341 gcggcatcga atggccccaa aaggtgatgg gttatttgat gacgatgagt gaaaaatcgg
     8401 gcgtgccgct ttattttgac agggcattgt aaaagtggct gttattaact gacagatgaa
     8461 ggaggttcta ccctatttga ggtaaactct atttaccatt ggccgaaata ccagtgctta
     8521 tctccaagac gaccttccca atcccttttg cctcttaaaa agaagtgact agtgtgtaaa
     8581 gttatagttc cttttatagc tttccaataa aattaaagta tttaaaagct tgtttagttc
     8641 taatttgtta atttctgtat ctaaataatg ttcagaatag tattattttt taaataaacc
     8701 atgaatagta cttcacattg tttgtcgtat tatttagctt ttaaatttct aatgtgaaag
     8761 atagtttaat ggatttcttt gatttttttg cgcaaggtaa gtataaaaaa attgcaactg
     8821 aacaaggttt agaccgtaaa tattttatat tccactatat cgtatgtaat ttatctgcca
     8881 tttcttcttt ttgtttaatg aaggttatcc atagttatac tatcctttgt ttattaataa
     8941 tgataattgg cattgcattt ggatttatct tgagaaatag ggttctatct atagtaaaaa
     9001 ataaacaaaa ttttttatct gatatgtttc ccttggaaat gcctttcttt tctagagtat
     9061 acaaaatacc tatcttggcc agattcttga ataggggaat gaaatggctt tatctccaaa
     9121 attttttctg tttagttatg atttgtttct cgatatctct tatccttaat gaagaaaggg
     9181 ggtggatgcc ttattttatc tgctattggt ttggactttg ttatccagca aacacttatt
     9241 acgttttttt atccagagat tataaacata tctaaactat tatgcgtggc ggtacttcca
     9301 aaggtaaatt ttaacctaat caatttactc aaaccatcca aaaattacca agcagcaata
     9361 taaacacaga tttatttgag ccaacctgat caaaacaagg aactaaatag atgaaaaaga
     9421 ccatttttaa cattaccaaa atggactgcc cgtccgaaga acagctcatt cgcatgcgtt
     9481 taaaagatgt gtctgatatt tatgaattac agtttgatat tgctgggcgg tgcttaaccg
     9541 tctatcacga taatcaagat acaacgattt tacaagtact tgaaccgctt aattttgata
     9601 gtcatatcat ttccaccgaa gtgattgttg ataagatagt gtttaacaag cctgatgagc
     9661 acttagaaaa aagattatta tatcaagtgc tcatgattaa ctttgtgttt tttatcatag
     9721 agtgttcggt tggtattttt gctaattcta tggggctgat tgctgatagt ctagatatgc
     9781 tggcggacag ctttgtctat atattggcgc tatcagccat tggtatgacg ctggcgtata
     9841 aaaaacgtgt ggcatttttg gcggggataa cgcagataat attggcgtta tttggggtga
     9901 tagaagtgat taggcgtttt atcggtgctg agcagctgcc caattatcaa ctgatgattg
     9961 gcacggcatt tttggcattg attgcaaact ggctgtgctt atatctactg agcaaaaatc
    10021 aaaataaaga aatccatatc aaagccagca tgatttttac atccaatgac attatcatca
    10081 atatcggtgt gatagcggcg ggagcattga cgttattgac tcattctagc tatcctgatt
    10141 taatcattgg catgattgtc tttgtcattg tactatttgg tgccagaaat attttaaaat
    10201 tggcaaaata attcctttca ccagcaaagg agtttttatg caaatatcgg tcaaagctac
    10261 ctacatacgt ggcagtactt ccaaaaactt gattttttaa aatgtggtta atgcgagttt
    10321 gtttatctgg tatttgattg gtcaagcctt catccaagcg tttgacgatt tcacgcaaaa
    10381 atacccctga tttacacaca gggtctaaga atgtcgcctt tggatttgac caaatctcag
    10441 acggagacct ttgcaaaat
//
LOCUS       DAXHTY010000065        10243 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXHTY010000065 DAXHTY010000000
VERSION     DAXHTY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.065"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            50..289
                     /locus_tag="ACFNMK_001447"
     CDS             50..289
                     /locus_tag="ACFNMK_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364698.1"
                     /translation="MNVWYIWDRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMSESPDFANVVAMAVAMICIVWIAVLSMTD"
     gene            504..1805
                     /gene="bioA"
                     /locus_tag="ACFNMK_001448"
     CDS             504..1805
                     /gene="bioA"
                     /locus_tag="ACFNMK_001448"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC6364699.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNLTSRFDDEWDEADLQPVRALFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKCRLKTAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            1802..2449
                     /gene="bioD"
                     /locus_tag="ACFNMK_001449"
     CDS             1802..2449
                     /gene="bioD"
                     /locus_tag="ACFNMK_001449"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC6364700.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSYPASPHLAARLDGRDLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLYSLVFNHIHDSRDAHIAQDSLNYLKCRLKADFSEAEWMELAKTDAV"
     gene            2464..2940
                     /locus_tag="ACFNMK_001450"
     CDS             2464..2940
                     /locus_tag="ACFNMK_001450"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC6364701.1"
                     /translation="MEHLFGKWLPDLPAAVSDGIDLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            2970..3860
                     /gene="ubiA"
                     /locus_tag="ACFNMK_001451"
     CDS             2970..3860
                     /gene="ubiA"
                     /locus_tag="ACFNMK_001451"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC6364702.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            4045..4494
                     /gene="ptsN"
                     /locus_tag="ACFNMK_001452"
     CDS             4045..4494
                     /gene="ptsN"
                     /locus_tag="ACFNMK_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC6364703.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            4496..5458
                     /gene="hprK"
                     /locus_tag="ACFNMK_001453"
     CDS             4496..5458
                     /gene="hprK"
                     /locus_tag="ACFNMK_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC6364704.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            5439..6293
                     /gene="rapZ"
                     /locus_tag="ACFNMK_001454"
     CDS             5439..6293
                     /gene="rapZ"
                     /locus_tag="ACFNMK_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC6364705.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            6374..7315
                     /gene="ylqF"
                     /locus_tag="ACFNMK_001455"
     CDS             6374..7315
                     /gene="ylqF"
                     /locus_tag="ACFNMK_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC6364706.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGKKPSEA"
     gene            7416..8018
                     /locus_tag="ACFNMK_001456"
     CDS             7416..8018
                     /locus_tag="ACFNMK_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC6364707.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            8202..9875
                     /gene="recN"
                     /locus_tag="ACFNMK_001457"
     CDS             8202..9875
                     /gene="recN"
                     /locus_tag="ACFNMK_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC6364708.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            9985..10061
                     /locus_tag="ACFNMK_001458"
     tRNA            9985..10061
                     /locus_tag="ACFNMK_001458"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10019..10021,aa:Arg,seq:acg)
     gene            10079..10153
                     /locus_tag="ACFNMK_001459"
     tRNA            10079..10153
                     /locus_tag="ACFNMK_001459"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10112..10114,aa:Glu,seq:ttc)
BASE COUNT         2476 a         2593 c         2789 g         2385 t
ORIGIN      
        1 atagggctgt aaccttcaat cggaatttgt tgcctgcggg atatacggta tgaatgtttg
       61 gtatatatgg gacaggatgg tggaaatcta tcataagtat aagaagccgt gcctggtttt
      121 ggcggtggat tttgtgatgg gtatggtatt catagagccg aatgaggagc cgtgcatcgg
      181 cagatgctat gcgccaatgt cggagtcccc tgattttgcc aacgttgttg cgatggctgt
      241 tgctatgatc tgtatcgtat ggattgccgt tttgtctatg actgattgaa gcgttgggcg
      301 gaggctgcgt gaaacggtat tgggcgttgg gccgtctgat tccaatcggg cttgaggaat
      361 gcgaaacggt gtgcgcttat gttgcggaac gatttgtttc gcggttttgc gcccgaaacg
      421 gatggaaagg tgtgggaagc ggtctgtcgg agtagaatac gcgttttgcg tttgaatgca
      481 gcaataagaa aagagagaaa cttatgccgt ctgaacatca acacacatca tcattgcttg
      541 atttcgaccg tacccatctg ctccatccct atacttccat gaccgatccg cttcccgttt
      601 atcctgtcaa acgtgcagaa ggggtgttta tcgaattggc ggacggcacg cggctgattg
      661 acgggatgtc gtcttggtgg tgtgcgatac acggttacaa tcatcccgtt ttgaatcagg
      721 cggttgagac gcagatgaaa cagatgtcgc atgtgatgtt cggcggtttg acgcatgagc
      781 cggcggtgga gctgggcaaa ttgttggtcg ggattttgcc gcaggggctg gatcgtattt
      841 tttatgcgga ttcgggttcg gtttcggtgg aagtcgcgtt gaagatggcg gtgcaatacc
      901 agcaggcgcg gggtttgcgt gcaaaacaaa atatggcgac ggtgcggcgc gggtatcacg
      961 gcgatacttg gaacgcgatg tccgtctgcg atccggaaac ggggatgcac catattttcg
     1021 gcagcgcgtt gccgcagcgt tattttgtcg ataacctgac aagccgtttc gacgatgaat
     1081 gggacgaggc ggatttgcag cctgtccgcg ccttgtttga agcgcatcat acggacattg
     1141 ccgcctttat tttggaaccg gtcgtgcagg gcgcgggcgg catgtatttt tatcatccgc
     1201 agtatcttcg cggattgcgc gatttgtgcg acgagtttga tatcgttttg atttttgatg
     1261 aaatcgccac cggattcggg cgcacgggca agatgtttgc ctgcgaacac gcggaggtcg
     1321 tgccggatat tatgtgcatc ggcaagggtt tgagcggcgg ctatatgacg ctggcggcag
     1381 caatcacttc gcaaaaagtt accgaaacga tttcgcgcgg cgaagcgggc gtgtttatgc
     1441 acggcccgac gtttatggca aacccgctgg cgtgtgccgt tgcctgcgct tcggtcaaac
     1501 tgcttttgtc tcaagactgg caggcaaata tccgccgcat tgaaagcatc ttaaaatgcc
     1561 gtctgaaaac cgcgtgggac attcgtggtg tgaaagacgt gcgcgttttg ggcgccatcg
     1621 gcgtgatcga gttggaaaaa ggcgtggata tggcgcgttt tcaagcggat tgcgtggcgc
     1681 agggcatttg ggtgcgcccg ttcggcaggt tggtttatct gatgccgcct tacatcattt
     1741 cagacggcgt tttgaccaaa cttgccgaca aaaccgtgca aatcttgaag gaacacagca
     1801 aatgaaaggc gtttacttcg tcagcggcat agacacggac atcggcaaaa ccgtcgccac
     1861 cggtatgttg gcaaaacaat tgttgcagca gggcaaaagc gtgatcacgc aaaagcccgt
     1921 gcaaaccggt tgccaagaca tcgccgaaga catcgccgtt caccgcaaaa tcatgggcat
     1981 acccatgcag gaagccgatg aacagcgtct gaccatgccc gaaatcttca gctatcccgc
     2041 ctcgccccac cttgccgccc gtctggatgg cagggatttg gacttggaca aaatccgtac
     2101 cgccacacaa gaattggcgg cgcagtacga agtcgttttg gtcgaaggcg cgggcggatt
     2161 gatggttccg ctgacggaaa acctgttaac cattgattat atccgtcagc aaggctatcc
     2221 cgtcatcctc gttaccagcg gacggcttgg cagtatcaac cacactttac tcagtttcgc
     2281 agcgctcaaa caatacggca ttcgcttgta cagcctcgtg ttcaaccaca tccacgacag
     2341 ccgcgacgca cacatcgccc aagacagcct gaactacttg aaatgccgtc tgaaagccga
     2401 tttttccgaa gcggaatgga tggagttggc aaaaacagac gcggtataaa gattgggaaa
     2461 aatatggaac acctatttgg gaaatggctg cccgacttgc ccgccgccgt ttcagacggc
     2521 atcgacctgc cgatgagccg gttattgaaa gcccggtcgc tgaccgccgc attgcgcgcc
     2581 ttgccgcata cattttcggt ggaactgctg aaactgggcg aattggagac ggaatgcgga
     2641 gggaggctgg tgcgcgaagt tttgttgaag ctggacggta tccctgttgt cgcggcaagg
     2701 agcgagtgcc gtatcggttc ggcgttttgg caaaacattt tggactgcgg cacgcgtcct
     2761 ttgggcgagc gtctgtttca agccgatttg gaaggggcgc gttcggcgtt tgagtttgcc
     2821 gtttccggcg aaggatgcgg acggtacttt gccgcgcggc gttctcggtt ttcccgtcac
     2881 ggcgaggaaa tgctgctgac cgagtatttt ctgcccgaac tgaaacgctt tatcggataa
     2941 aataccgttt tttcaagctg tgcggcaata tgaatcctaa atccccttta tttttacgac
     3001 tgtccgatcg tttggatgtg tacctgcgcc tgatgcgggc ggacaagccc attgggacgc
     3061 tgcttttgct gtggccgacc tactgggcat tgtggctggc ttcagacggc attcccgatt
     3121 tggcggtatt ggcggcgttt acaatcggca cgtttttaat gcggagtgcc ggttgcgtca
     3181 tcaacgattt tgccgaccgc gattttgacg gtgctgtcga gcgcaccaaa aaccgtccgt
     3241 tcgcacaggg tcgggtcgga aaaaaagaag cgctgctgct gacggcattt ttgtgcctgc
     3301 ttgccgcatt gtgcctgatt ccgctgaatc atctgacatg gctgatgagc ctgcccgcgc
     3361 tgtttcttgc gctgacttac ccgtttacca aacgcttttt tccgattccc caactctatc
     3421 tcgggcttgc cttttccttc ggtatcccga tggcgtttgc cgccgttgcc ggaaacgtgc
     3481 cgcctcaagc gtggatactc tttgccgcca atgtgttatg gactctggcg tatgacacgg
     3541 tttatgcaat ggcggacaaa gaagacgatt tgaaaatcgg catcaaaacc tccgccgtca
     3601 cgttcgggcg ttacgacatc gccgccgtta tgctgtgtca tggcggcttt accctgctga
     3661 tggcagtatt gggtgcggtt atcggtgcgg catgggcata ttggacggca atccccatcg
     3721 tcctgctgct gcaataccgc caatatgccg ccatcaaaag ccgcgtccgg caaatctgtt
     3781 ttgaaacgtt tttggcaaac aacagaattg gttgggtgtg gtttaccgcc atttttgccc
     3841 atacgttttt cgcgaaataa ggcagggcaa tgccgtctga agagccgtaa actgctttgg
     3901 acggcatttc tatctgtgcc gaaaagcgtt aaaatatgtt tttaaaacgc tgtgttatgt
     3961 cagcccgtac cgtatgcggg attgagattt gccctagcag tcggtacaat ctttctgttt
     4021 tgcgatgtct gaaaagagaa gcttatgagc cttatcggcg aaattttgcc tttgtcccat
     4081 attgttttgg atatggaggt gggcagtaaa aaaaggctgt ttgaggaagc gggcctgctt
     4141 ttggaacgcg aatcctcatt gtcccatgct gatgttttcg aatgtctttt tgcccgtgaa
     4201 aaactcggtt cgaccggttt ggggcagggc gttgccatcc cgcacggccg tcatgccggc
     4261 gtgaagcagg cgacgggcgc gttcatccgc acgcgcgaac ccgtcggatt tgacgcaccg
     4321 gacggcaagc cggtttccct gatttttatc ttgctggtgc cggaaaacgc aactggcgaa
     4381 catttggaag tcttatccaa actggccggc aagttttccc aaaaaagcat ccgcgaatcg
     4441 ctgatgacgg tttcttctgc ggaagaagcc cgcgccattt tgactgaaga ataatatgcc
     4501 cagtatttcc gtccgccgac tgtttgacga caaccaatac aagttacaac ttgcctgggc
     4561 cgccggcaat tcgggtgcgg acaaccgtat cggcgtagag gcggacaagc ccgtcctcgc
     4621 tttggtcgga cacctgaatt tcatccaccc caaccaaatc caagtggtcg gtttggcgga
     4681 atcggaatat ctgaaccgtc tcgaatcggg ggaaacgggt tatcagttcg gcgacctgtt
     4741 cgacatttct atgtctttgg ttattgtggc aaacggcttg ccggtttccc cggggctgcg
     4801 cgactattgc cataaaaacg atattccact gctgacttcc aaactcgaaa gcccctatct
     4861 gatggacgtg ttgcggattt acctgcaacg caccctggcg gcatcgtccg tcaaacacgg
     4921 cgtatttctc gatgtgtttg aaatcggcgt gctgattacc ggacattccg gcttgggcaa
     4981 gagcgaattg gcgttggaac tgatttcgcg cggccacagc ctgattgccg acgatgcggt
     5041 cgagctgttc cgcatcggtc cggaaacatt ggaagggcgt tgttcgccta tgctgcgtga
     5101 ttttttggaa gtgcgcggct tggggatact caatatccgc catattttcg gcgaaacttc
     5161 catccgcccc aaaaaaatcc tgcaactcat tatcaatttg gtcgaggcag acgacgagta
     5221 tatgaagcag ctcgaccggt tgagtatccg caccgaaacc gaatccatcc tcaacgtcaa
     5281 cgtccgttcg gttacgctgc ccgtcgccgt cggacgcaac ctcgccgttt tggttgaggc
     5341 ggcggtacgc aattacattt tgcagttgcg cggtaaggac agtacgcgcg aatttttgga
     5401 acgccatcag acgcaactta aagaaaacga acagaatcat gaaaatcgtc ctgattagcg
     5461 ggttgtccgg ttcgggcaaa tccgtcgcac tgcgccaaat ggaagattcg ggttatttct
     5521 gcgtggacaa cctgcccttg gaaatgttgc ccgcgctggt gtcgtatcat atcgagcgtg
     5581 cggacgaaac cgaattggcg gtcagcgtcg atgtacgttc cggcattgac atcggacagg
     5641 cgcgggaaca gattgcctct ctgcgcggac tggggcacag ggttgaagtt ttgtttgtcg
     5701 aggcggaaga aagcgtgttg gtccgccggt tttccgaaac caggcgagga catcctctga
     5761 gcaatcagga tatgaccctg ttggaaagct taaagaaaga acgggaatgg ctgttcccgc
     5821 ttaaagaaat cgcctattgt atcgacactt ccaagatgaa tgcccaacag ctccgccatg
     5881 cagtccggca gtggctgaag gtcgaacgta ccgggctgct ggtgattttg gagtccttcg
     5941 ggttcaaata cggtgtgccg aacaacgcgg attttatgtt cgatatgcgc agtctgccca
     6001 acccgtatta cgatcccgag ttgaggcctt acaccggtat ggacaagccc gtttgggatt
     6061 atttggacgg acagccgctt gtgcaggaaa tggttgacga catcgaaagg tttgttacgc
     6121 attggttacc gcgtttggag gatgaaagca ggagctacgt taccgtcgcc atcggttgca
     6181 cgggaggaca gcaccgttcg gtctatattg tcgaaaaact cgcccgaagg ttgaaagggc
     6241 gttatgaatt gctgatacgg cacagacagg cgcaaaacct gccgagccgc taatcccgtt
     6301 gaacgatgat gccgtctgaa acccgatttt ctgtttcaga cggcatttca aatattgaga
     6361 agaaaggaaa gacatggcaa tccaatggtt tcccggccat atgaacaaag cgaaaaaagc
     6421 catcgccgag cgcgccaaaa gcgtagacat ggtgattgaa atgctggatg cgcgaatgcc
     6481 cgcctccagc gaaaaccccc tgcttgccca gctttccaaa ggcaaaccca aacttaaaat
     6541 cctaaacaaa caagatcttg ccgaccccga acgcacaaag gtctggcttg accactacaa
     6601 cagccgcccc gacacgtgcg ccattgccct cgattcctcc gaaaccggcg cacacggcaa
     6661 aatcacccaa gcctgccgcg cgatgattcc ccatcggcaa ggcatagaca aacccttgcg
     6721 cgtcttgatt tgcggcattc ccaacgtcgg caaatccacc ctcatcaacg gcatgatagg
     6781 caaaaaatcc gccaaaaccg gcaacgaacc cggcatcacc aaagccgaac aacgcctttt
     6841 ccttgccgac gacttctggc tgtacgacac ccccggaatg ctgtggccga aaatcattgt
     6901 cgaagaaagc ggctacaacc tcgccgccgg cggcgcagtc ggacgcaacg cattggacga
     6961 agaagaagtc gccctcgaac ttttagacta cctccgccgc cactacctcc ctatgttgca
     7021 agaacgctac caagccgaca aagaccccag cagccactgg gacgaaaacg tttggctcga
     7081 atggatagcc aaaaaacgcg gcgcagtcct cagcggcgga cggatcaact accaaaaagc
     7141 cgccgaaaac atcctcaccg acttccgtga aggcaaaatc ggcagaatca ccctcgaaac
     7201 gccgaaccaa tgggaaactt ggctcaaaaa agcccgtcag aaagaagccg aactcaaagc
     7261 catacgcgaa gccagaaaag cagagcgtaa agggaagaag ccttcggaag cataaagaat
     7321 gccgtctgaa aaatattttt caggcagctt ctctctactc caaccgattt cagacggcat
     7381 atccaaaccc atgccgtttc agcacggata cccgtatgac cgacaaaatt tctcccgacg
     7441 cgctgattga agccgcactg ctgacccaaa ccgaaccgct gaccgaaaaa tctatgcgcg
     7501 aattgtgtgt gccgccactg tcgcaggaca aactgattga tgtgttggcg cagttgaaaa
     7561 cgcgttggca ggatagggcg ttgcaactgg tgcatacgca agagggctgg cgttttcaga
     7621 ttgttcagac ggcattcgag cggctgggca gcctgcaaga acagcgtgcg ccgcgctact
     7681 cccgcgccgt gatggaaaca ctggcgatta tcgcctacca gcagcccgta acgcgcggcg
     7741 acatcgaagg catacgcggt gtggcggtgt cgcagaacgt gatgcagacc ttgcaggatc
     7801 gggggtggat tgaagtcatc ggacatcggg acacattggg aaaacccgca ttgtgggcga
     7861 caacggcaac ttttctcagc gatttgcgct tggacggtct ggaagaactt ccgccgctga
     7921 ccgaactggg cgaactggtt ttacccgatt taatggaaat gccgcctacg gatgaagaag
     7981 agccggaagc cgtaccgtcc gataccctgc ccaactgaaa ttccaaatgc cgtctgaaac
     8041 gcacattgct tcagacggca tttcaacaaa taagcagata aaaacaagca ctaagcaaaa
     8101 ttaaggaaaa acttattttt aatttaaaaa atcttagtta taattcgtat atctaaagtt
     8161 gatattgctt ttgtcggtag aattgctaag gaatcctcac gatgcttcta acactttctt
     8221 tgcgtgattt tgtcattgtc gaaaatctga atctggattt tcaaagcggc tttaccgtat
     8281 tgaccggaga aactggcgcg ggcaagtcca ttactttgga tgcgattggt ctgctgttgg
     8341 gcgataaagc cgattacagc caagtccgca gcggcgcaaa agaagcgcag ttgtcggcgt
     8401 tgtttgatat ttcccattta cctgttttaa aagcagaatt gtatgaacag gggcttttaa
     8461 acgacggaga agaagaactc agtatccgcc gcattatcga tgccaaaggc aaaagccgca
     8521 gctttatcaa caatcaggcc gctaccttgg cgcaactcaa agccgtcggc agccagctta
     8581 tcgacatcca tgggcaaaac gcccatcatt cgcttaatca ggaagccgcc cagcgcgaat
     8641 tgttggacgc atttgcgggc agcagggcgc aggcggaaac cgtcaggcag ctttatcaaa
     8701 actgggcaaa ctcaaaaaaa gcattgcagg aagcgcagga acaggcagaa aacattgcac
     8761 tcgaacaaga gcgtctggaa tggcagttta acgaattgaa tcagttggac attaaacaag
     8821 gcgaatggga agccctcagc caaagccacg acagccttgc ccattctgcc gagctgttgc
     8881 aggctgccga agaagtcgga agcaagattg acggcgacaa cggcatccaa cgccatatct
     8941 atcaatgtca aaaactattg gccaatctgc aaaacatcga gccgcgcttt gccgagagcc
     9001 tgaatatgtt ggcaagcatc gaagccgaat tgggcgaaat cagtgccaat atgcgcgatg
     9061 tggcaggtcg cagcgacatc aatcccaacg aacttgccgc acaagagcag cgtatgggcg
     9121 agctgatggg gatggcgcgg aaataccgga tcgagcctga agagttgcct gccaagttgg
     9181 cagaaatcga agaacgcctg caaagcctgc aagctgccgc cgatttggac gcgctcgagc
     9241 ataatgttgc ccacaatttt gccgaatatc aggaagctgc ccacatcctt tctgccatgc
     9301 gccatcaggc ggcagagcgt ttgagcggcg aaacgaccga gcatatgcaa caccttgcca
     9361 tgaaaggtgc acgtttcgac atcgtcctgt tgccctcgtc gccgacggca cacggtttgg
     9421 agcaggttca atttcaagtt gccgccaaca aaggcaatcc gccccgtccg ctgaataaag
     9481 ttgcctccgg cggcgaattg gcgcgtatca gccttgcctt acaggttgtt gccagccaat
     9541 atacccaagt tcccaccctg atttttgatg aggtcgatac cggtattgga gggggagtgg
     9601 ctgaaatggt cggcaaggca ttacgtgcgt tgggcagaaa acatcaggtg cttgccgtta
     9661 cccatcttcc gcaagtcgca tcctgcggcg aaaaccactg gcgagtgcgc aagcacagcg
     9721 agggagagca aaccgtcagc gaaatcagta tattggatga aatccaacgg gtcgaagagg
     9781 ttgcccgtat gttgggcgga gaagtcatta ccgatacgac gcggcaacat gcggcagaat
     9841 tgctgcaact tgcgtcgaaa aatagtttat tttaaaatca atcagttaaa aaataactaa
     9901 aaataaaagt ctaaaacaat agacagaact cagataaatc cgtattatta cgctttctta
     9961 atcacttgaa caagtgattg tgctgcaccc gtagctcagt tggatagagt atctggctac
    10021 gaaccagagg gtcgggcgtt cgaatcgctc cgggtgcgcc agtaagaaaa tacaatacgc
    10081 gcccatcgtc tagcggttag gacatcgccc tttcacggcg gtaaccgggg ttcgattccc
    10141 cgtgggcgtg ccaattcaaa atgcctccga ttatatcgga ggcatttctc atttctcatt
    10201 tctcatttct catttctcat ttctcatttc tcatttctca tac
//
LOCUS       DAXHTY010000066        10101 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXHTY010000066 DAXHTY010000000
VERSION     DAXHTY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.066"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(102..1109)
                     /locus_tag="ACFNMK_001460"
     CDS             complement(102..1109)
                     /locus_tag="ACFNMK_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6364709.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDNTLSELLELINRQLTEKNLKIEKASAAVIDATIIQTAGSKQRQAIE
                     VDEEGKVSGQTTPSKDKDARRTKKNGLYKLGYKQHTRTDEEGYIEKLHITPANAHECK
                     HLPPLLEGLPKDTTVYADKGYDSAENRQHLKEHRLLDGIMRKAQRNRPLTEAQTKRNR
                     YLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSVPVA
                     A"
     gene            1150..1989
                     /locus_tag="ACFNMK_001461"
     CDS             1150..1989
                     /locus_tag="ACFNMK_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364710.1"
                     /translation="MGNFAKVSNCEFWDKINYNRAAQAAEQRAKQAQETPKEPPKQEE
                     HFIAKLPNDKLMTIYRLFQKQLDDMRGLERDIERGDWYINARYRMNENRNKIRALEDD
                     NKRLHHDYWAVQDQGENRFFGMGKIGAKLVKENEKQQHINERHGANVREIRRLKTENE
                     KLEGQYSDKLAADVVKVRDRFNEELDNVRREIGKRPMEEFRNVFLQAAQEAQTTIGQK
                     AVQIELEKFPLNGSPSAKRIAFYFGAQRIREVEQKEREDQRKKQERQARRMERDDGHG
                     IGM"
     gene            complement(2259..3521)
                     /locus_tag="ACFNMK_001462"
     CDS             complement(2259..3521)
                     /locus_tag="ACFNMK_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC6364711.1"
                     /translation="MTLFRPEVFQAKKNRWTGQIVLVRPFSLQFLTFFAVSLAAILVA
                     FLIFGSYTNKTTVTGQLLPTTGVVRVYSQDMGVIAHQHVMNGDFVKKGDVLFTLSTSR
                     NDNNGSIQARLLAEAELKKSLSEQEIIMKKRVHAAEKTAQENTVHRFQNQMQHVRNQI
                     IMQEKRIAISEKMLEKQRYLAKMDAISELEKNSYEIALLELKAGLAAYQREADNLARE
                     ITVQQSNLKNLPEQQATEISQLERAVSVYQQEILDYQQRNEQTIRATISGYVSSINTE
                     IGQQVDTNKLLMSIVPKESELLANLYVPSRAIGFVKPNDKVILRYQAYPYQKFGHAEG
                     HVISIAQTALGAQEWTNLGNIFTQTAQVNEPVYLIKVKLDNQHIRIYGTEKKLQIGMI
                     LEADILHENKRLYEWILDPLYQVMGKIS"
     gene            complement(3523..5619)
                     /locus_tag="ACFNMK_001463"
     CDS             complement(3523..5619)
                     /locus_tag="ACFNMK_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFC6364712.1"
                     /translation="MPTYLSRLSFSFSRRLPVILQTEAAECGLACLVSVLGFHGFYTD
                     LRHLRARFSLSLKGATLADLVRFANSMNLTARAVRLDLDELANLRVPCILHWDLNHFV
                     VLHEVHRNHIMIMNPASGWQKVRMEEVSRCFTGVALELFPNTQFEEKHETRKIRVLPM
                     LRGVVGLKRSLFQLLLLATVLQVFALASPFFMQWVIDHAIVSADRDLLLTLALGFGLL
                     MIVQQLVSLLQTWAGMYLSTSLNIQWKANVFSHLMDLPISYFAKRHLGDVVSRFGSVD
                     SIQSTLTSTFFVLVINSILAVFTLGLMYLYSPSLMAVVLVVLLIYIGIRWVAYYPLRH
                     ATEENIVHAAKQSSYFMETIRGIQTVKLFDKNTQRHAAWLNLFIDTINTGLTTQKLSA
                     LFGFANSLLFGIANILIVYFGAVSVLNGGFTVGALMAFMSYKSQFESKAGSLIDQFVQ
                     IKMLGLHAERLADIVLEETENEQNNDFSLPKNLEHFDIQIENVSFSYAENEPYILQNF
                     SLIIKQGTAIAFAGHSGCGKSTLIQILTGSLKPESGHVLLGGHDIHALPPAFIRTWSA
                     SVMQNDVLFTGSIAENISFFDDTPNMEKIAFCAQMANIHHEIVAMPMAYETLIGDMGS
                     ALSGGQKQRIVLARALYREPKILFLDEASSHLDINNERVINDNLRQLNITKIMVAHRQ
                     ETLNTADSVVYLDKVA"
     gene            complement(5836..6171)
                     /locus_tag="ACFNMK_001464"
     CDS             complement(5836..6171)
                     /locus_tag="ACFNMK_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364713.1"
                     /translation="MYELSIVDLELVSGAGPIADFTTGEGGAIGGGTGYIAGKAGGGT
                     IGGILGSPFAPGGTIVGVAAGQQYGGKVGSVIGAGAGSAIGGVIGRGIESILDNDRSG
                     NDYGDGTGY"
     gene            complement(6764..7099)
                     /locus_tag="ACFNMK_001465"
     CDS             complement(6764..7099)
                     /locus_tag="ACFNMK_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6364714.1"
                     /translation="MKELNISDLKIVSGGAEPAYINPLDAIGIGSAAVGGGRAVSATA
                     TGDMIARSAARGGLIGLAGIAGWNTGTYLYHETPLGDWLSSGVDKLTGLDQNFNDASG
                     NDYGDGTGY"
     gene            complement(7341..8489)
                     /locus_tag="ACFNMK_001466"
     CDS             complement(7341..8489)
                     /locus_tag="ACFNMK_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6364715.1"
                     /translation="MHINFSMEYKEFNENYLYKKPFIFKNALDVSSISWKEINELYQR
                     ADPTDWQFKFRKGEIIPKEAYVESFNDVGKIRYRFNKTAVYQYLQDGATMVYNRIDNE
                     PFVDSIAKQIAQFAQAQTVVSGYLAFGSSSSYRNHWDTRDVFAVQLIGTKHWTLSAAN
                     FDMPLYMQQAKDIPHITPPTTVDMEVILEAGDILYIPRGWWHNPMPMNCETFHLAIGT
                     FPPNGYNYMEWLMKKIPDIQSIRQNFIGWQHDQKNLDDAAQAITEMMNNPKNYQTFMQ
                     DFLGSQRTNTAFNMDLFGNAHNQTLPEHCFLRLNSNDYSTISQGYLIANGIKLNIDEI
                     SMEFLMILVDKYMISLAEILLFFSVDEEENIKKLVWRLGELDIIEIIR"
     gene            complement(8665..8868)
                     /locus_tag="ACFNMK_001467"
     CDS             complement(8665..8868)
                     /locus_tag="ACFNMK_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFC6364716.1"
                     /translation="MNGLKADRPSANKANLSIDDVIKQEEIKRISFDMSESLHRELKK
                     YAAENGVTIRVVLNKLVSDLLNK"
     gene            complement(8911..9318)
                     /locus_tag="ACFNMK_001468"
     CDS             complement(8911..9318)
                     /locus_tag="ACFNMK_001468"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFC6364717.1"
                     /translation="MIDGAPQTADLAISAIKASDLVLIPVQPSPYDIWATADLVNLIK
                     QRIKLIDGNLKAAFLINRTIKGTKIGGEIRAALEEYELPVLSTVIVQRIIYPTSAITG
                     STVLDKEPNGEAAKEIQSLANEIINLINIDNHI"
     gene            complement(9331..9555)
                     /locus_tag="ACFNMK_001469"
     CDS             complement(9331..9555)
                     /locus_tag="ACFNMK_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6364718.1"
                     /translation="MKVISVLNQKGGSGKTTVATHLARALQLAGNSILLVDSDPQGSS
                     RDWAAVHEEQPVPVIGIDSPTIDRDLKSVV"
     gene            complement(9902..>10101)
                     /locus_tag="ACFNMK_001470"
     CDS             complement(9902..>10101)
                     /locus_tag="ACFNMK_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6364719.1"
                     /translation="QTKHNRYLSKIRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSH
                     LKAMCLNLLKAANRLSAPAAA"
BASE COUNT         3101 a         2101 c         1844 g         3055 t
ORIGIN      
        1 acaggttttc ggctgttttc gccccaaata cctcctgatt ttacccaaat acccccttaa
       61 tcttccccgg acgccccata atcaggcatc cgggccgcct tttaggcggc aacaggcaca
      121 cttagcctgt tagctgcttt caacaggttt aaacacatcg ccttcagatg gctttgcgca
      181 ctcactttaa tcagcccgaa ataggctgcc cgggcgtagc ggaatttacg gtgcagcgta
      241 ccaaagcttt gttcgaccac ataacgggtt ttcgacaaat atcggttacg tttggtttgc
      301 gcttccgtca gcggacggtt acgctgggct ttgcgcataa tgccgtccag caaccgatgt
      361 tctttcaggt gttgccggtt ttccgcactg tcatagcctt tgtcggcata gacggtcgta
      421 tctttgggca gtccttccaa caaaggcggc aggtgtttgc actcatgggc attggcgggg
      481 gtgatgtgca gtttctcgat atagccttcc tcatcggtac gggtatgctg tttgtaaccg
      541 agtttgtaga ggccgttttt ctttgtccgg cgggcatctt tatctttact cggtgtggtt
      601 tggccgctga cttttccttc ttcatcgact tctatagcct gacgctgttt gctgccggcg
      661 gtctgaataa tggtggcgtc aatgacggcg gcggatgctt tctctatttt taggtttttt
      721 tcggtcagtt gtcggttaat cagttccagc aattcggaca gggtgttgtc ttgcgccagc
      781 cagttacggt agcggcataa ggtgctgtaa tcggggatgc tcagttcgtc aaaacgacaa
      841 aacaggttga aatcgatgcg ggtgatgagg ctgtgttcga gttcgggatc ggaaaggctg
      901 tgccattgtc cgagcaggac ggctttgaac atggataaca ggggataggc gggacggcct
      961 cggtggtctc gaaggtaacg ggttctttga cgattcaggt actgttcgat cggttgccaa
     1021 tcaatcacct gatccaactt caatagtggg aagcggtcga tgtgtttggc aatcatggct
     1081 tgtgcggttt gctggaagaa ggtactcatg agaaatcccc taaatgtctt agtaggaatt
     1141 taggggattt tggggaattt tgcaaaggtc tcaaattgtg aattctggga taaaattaat
     1201 tataatcggg ctgcccaagc cgccgagcag cgggcaaagc aggcgcagga aacaccgaaa
     1261 gagccgccga aacaggaaga gcactttatc gccaagctgc cgaacgacaa gctaatgacg
     1321 atatacaggc tgtttcaaaa acaactggac gacatgcgcg gcttggagcg cgatatagaa
     1381 cgcggagatt ggtacatcaa tgcgcgatac cgcatgaacg aaaaccgaaa caaaataagg
     1441 gcgctggaag acgacaacaa acggctgcat cacgactatt gggcagtcca agaccaaggc
     1501 gaaaaccgct ttttcggcat gggtaaaatc ggcgcaaaac tggttaagga aaacgagaag
     1561 cagcaacaca tcaacgaacg gcatggagcg aacgtgcgag aaatccggcg gctgaaaaca
     1621 gaaaacgaaa aactggaagg gcagtatagc gacaagttgg ccgccgacgt ggtcaaagta
     1681 cgcgacaggt ttaacgaaga actggacaat gtccgcaggg aaatcggcaa acggccgatg
     1741 gaagaattca ggaacgtatt tttgcaggca gcccaagaag cacagacgac aatagggcag
     1801 aaggcggttc aaatcgaact ggaaaaattc cccctgaacg gcagcccttc ggccaagcga
     1861 atcgcgtttt atttcggcgc gcagcggata agggaagtag agcagaagga gcgggaagac
     1921 cagcgcaaaa agcaggagcg gcaggcgcgg cgtatggagc gggacgacgg ccacggcatc
     1981 ggcatgtaga aaaaccagcc tatcatactg gttggaagac gtattatggt acaaagtcag
     2041 caaatgcatc gaaaatattt acttctcgca tacaacgcag atacaattgc agcaattcag
     2101 gaagcaaaaa tggagcgaaa aattgtatca cttctctgtc ttcattatta cataaaacac
     2161 tgtgtatttg tgcttaacaa caaattggaa gtcatcggac attggttttg cttgataatt
     2221 aggggcactc atacgctcca atgtattgag caattcagct atgatatttt tcccattact
     2281 tgatacaaag ggtcaagtat ccattcatat aaccgtttat tctcatgcaa aatatcggct
     2341 tctagtatca taccaatttg caatttcttc tccgtgccat atatacgaat atgctgatta
     2401 tccagtttta ctttgattaa ataaacaggc tcattcactt gtgctgtttg agtaaaaata
     2461 ttgcctaaat ttgtccattc ttgcgcccct agtgccgttt gagcaataga aatgacgtgt
     2521 ccttctgcgt gtccgaattt ttgataagga tatgcttgat aacgcaaaat taccttgtca
     2581 tttggtttta caaaaccgat tgcgcgacta gggacatata aattagccag taattcactt
     2641 tctttcggca cgatactcat caatagtttg ttagtatcta cctgctgacc aatttccgtg
     2701 ttaatagaac tgacatagcc tgatatggtt gcgcgaatag tttgttcgtt acgttgttga
     2761 taatccaata tttcttgttg atagacagat actgcgcgtt ctaattgact gatttcagta
     2821 gcttgctgtt caggcagatt ttttagattg ctttgctgta cagttatttc ccgagcaaga
     2881 ttatctgctt ctcgttggta agcggctaaa cctgctttca gctccaataa agcaatttca
     2941 taactatttt tttctaattc agaaattgcg tccattttgg ctaaataacg ctgtttttct
     3001 aacatttttt cagaaatcgc aatacgtttt tcttgcatga taatttgatt tctaacgtgt
     3061 tgcatttggt tctgaaaacg gtgtaccgtg ttttcttgtg cggttttttc tgctgcatga
     3121 acacgttttt tcataataat ttcttgttct gatagtgatt tttttaattc agcttcggcg
     3181 agtaatcgtg cttgtatgct gccattattg tcatttcgtg aagtagataa tgtaaataac
     3241 acatcgcctt ttttcacaaa atcgccattc ataacgtgtt gatgagcaat aacccccata
     3301 tcctgcgaat atacgcgtac tacccctgtt gtcggcaaaa gttgtcctgt tacggtggtt
     3361 ttattggtat agctcccaaa aattaaaaaa gcaactaaaa tagccgctaa tgagaccgcg
     3421 aaaaatgtta aaaactgcaa ggaaaaagga cgcactaaaa caatttgccc tgtccagcga
     3481 ttttttttag cttggaatac ttcggggcga aataatgtca tttcaggcta ccttgtctaa
     3541 ataaactacg ctatcagcag tatttaaagt ttcctgacga tgcgcgacca tgattttggt
     3601 aatgttgagt tggcgtagat tatcgttaat cacacgttca ttgttgatgt ccaaatggct
     3661 actggcttca tctaaaaaca aaatttttgg ttcacgatat aaagcacgag ctagtacgat
     3721 gcgctgcttt tgcccaccac tcaatgcact ccccatgtcg ccaatcaatg tttcatacgc
     3781 cataggcata gcgacaattt catgatgaat attcgccatt tgagcgcaaa atgcgatttt
     3841 ttccatattg ggcgtatcat caaaaaaact gatgttttct gcgattgagc cagtaaataa
     3901 cacatcattt tgcatcacac ttgcgctcca tgtgcggata aatgcaggtg gtaacgcatg
     3961 aatgtcatgc ccacctaata acacatgacc gctttcaggt ttcagactgc ctgtcagaat
     4021 ttgaatgagc gtagatttcc cacagcctga atgtccagca aacgcaatgg ctgttccttg
     4081 tttgatgatt aaactgaaat tttgcaaaat gtatggttca ttttcagcat agctaaatga
     4141 tacattttct atttgaatat caaaatgttc taaattttta ggcagactga aatcgttgtt
     4201 ttgttcattt tccgtttctt ctagcacaat atccgccaag cgttccgcgt gtaatcctag
     4261 cattttgatt tgtacaaact ggtcaatcaa tgaacctgct ttactctcaa actgactttt
     4321 gtaggacata aacgccatta acgccccaac ggtaaaacca ccgttcaata cagaaaccgc
     4381 accaaaatac acaattaaaa tattagcgat accaaataaa aggctgttcg caaaaccgaa
     4441 cagcgcggaa agtttttgtg tcgttaaacc tgtgttaatc gtgtcaataa ataaattcag
     4501 ccaagccgca tgacgttgtg tatttttatc aaataattta accgtttgaa tacctcgtat
     4561 ggtttccatg aaataactgc tctgcttggc agcatgaaca atattttctt cggttgcatg
     4621 gcgtaatgga taatatgcca cccaacgaat accaatataa atcagcaaaa caaccagcac
     4681 aactgccatt aaactcgggc tgtacaaata cattaatcct aatgtaaata cggccagaat
     4741 gctgttgatc accagcacaa agaaagtaga agtgagcgta ctttgaatac tgtccaccga
     4801 gccaaaacgc gaaaccacat cacctaaatg tcgtttagcg aaataagaaa taggcaaatc
     4861 cattaagtgg ctgaatacgt tagctttcca ttggatattc aaagaggtag ataagtacat
     4921 acccgcccaa gtttgcaaca atgacactaa ctgctgcaca atcatcaaca agccaaaacc
     4981 caatgccaat gtcagcaata aatcacggtc tgccgataca atggcatggt ctattaccca
     5041 ttgcataaaa aaagggcttg ccaaagcaaa cacctgtaat acagtagcca acagtaaaag
     5101 ctgaaataaa gatcttttta atcccaccac tccgcgcaac attggtaata cgcgaatttt
     5161 acgcgtttcg tgtttctctt caaattgcgt gttaggaaat agttccaacg ctacacctgt
     5221 aaaacagcgc gagacttctt ccatacgcac tttttgccaa cccgaagcag ggttcataat
     5281 cataatgtga ttgcgatgaa cttcatgcaa caccacaaaa tgatttaaat cccaatgcaa
     5341 aatacagggt acgcgcaaat ttgctaactc atctaaatcc aaccgaacag cccgagcggt
     5401 taaattcatg ctattagcaa accgtaccaa atccgccaat gttgcgcctt taagcgacaa
     5461 agaaaaacgc gcccttaaat ggcgcaaatc cgtataaaaa ccatgaaacc ccaacaccga
     5521 taccaaacac gctaatccac attcggctgc ttccgtttgc aaaataacgg gcaaacggcg
     5581 cgagaaactg aatgataggc gagagagata tgttggcatt agagttccta ttttattttt
     5641 tatttaaaaa aattattatt accgataata atactgataa taaaaaaaat aaaactattt
     5701 tcatttgatt acgacttaat ggaggctaat gtattaatcc gtaatgtaca gataagccaa
     5761 ccaaaaaaat caaaacaaga aagaaagcca tgagtacttg gtaaggattc atcaatgcta
     5821 tccctataat tttgtttaat accctgttcc atcaccgtaa tcattacctg atcgatcatt
     5881 atccaaaatg ctttcaatac ctcgtccgat cacaccacca atggcagatc cagctcctgc
     5941 cccaattaca gaaccaacct taccaccata ttgttgtcca gctgcaacac caactatagt
     6001 accacctgga gcaaatggag agcctagaat tcccccgatg gttccaccac ctgctttccc
     6061 tgcaatatat ccagtaccac caccaatagc acctccctct cctgtagtga aatcagcaat
     6121 gggaccagca ccactaacta attcaagatc cacaatagag agttcataca tttttattta
     6181 cctcaaaata aaatttaata ataaaaaaga aatgagttag acagaatagt atcactataa
     6241 aaatgataga ataaatattc atattaaaat aaaaattttc ctgtctaaat agagttatta
     6301 aggcaatata caaaataacc ataaaataaa aagtgaatat ttttacaaat tctattttat
     6361 tttgattttt atttattcct cgtgcaaaaa ttaccatttt ataaatcaaa agcaatgttg
     6421 aaattataaa cgaaaatgct attaatatag aaatatgatt tgcaaaaggt atagttgata
     6481 aattaaatct aactttactg tatacaatgt atacgttcaa taaaataaat gatattgagg
     6541 atatattgat taaaattttt gtgttattta ataaaacacc cattgattac ctcacgattt
     6601 caataacatc taataaacat aactccctaa ctaatttctt aatattttct tgttcatcaa
     6661 cactaaacaa cgataatatt tcagctaggg atatcatgta aagcaagaaa gaaagccatg
     6721 agtacttggt aagcattcat caatgctatt cctataattt tgtttaatac cctgttccat
     6781 cgccataatc attaccagat gcatcattaa aattttggtc tagtcctgtt aatttatcaa
     6841 ctcctgaact taaccaatcg cctaatggcg tttcatgata taaataagta cctgtattcc
     6901 agcctgctat gcctgccaaa ccaattaatc cacctcgagc agcacttctt gctatcatat
     6961 ccccagttgc agtagctgaa acagctctcc ctccacctac cgctgcacta cctataccaa
     7021 tagcatcaag aggattaatg taagctggtt cagcaccacc tgaaactatt ttcaggtcag
     7081 aaatatttaa ttctttcatt gcttttcctt tttaacaatc ttatttttca atatgattga
     7141 atagtacgct catgccaata ccaatcataa tactaaatac tatattaatg taatctccgt
     7201 cataattagg atgaagacgc caccctccta attctgataa tttcataaaa atatatatta
     7261 caatagaata tccaattgca tgtaaaaatg ttttcatata ttttatatcc taaaatatta
     7321 aatcaaatat taaatccatc tcatcttata atttcaatta tatctaactc tcctagccgc
     7381 caaactaatt tcttaatatt ttcttcttca tcaacactaa aaaacaataa tatttcagct
     7441 agggatatca tgtatttatc aaccaaaatc attaaaaatt ccatactgat ttcatctata
     7501 tttaatttaa tgccattcgc aatcaggtat ccttgtgaaa tagttgagta atcattacta
     7561 tttaatctta aaaagcaatg ctcaggtaat gtttgattat gagcatttcc aaacaaatcc
     7621 atattgaatg ctgtattggt acgctgactt cccaaaaaat cttgcataaa tgtttgataa
     7681 ttttttgggt tattcatcat ttcggtaatt gcttgagctg catcatctag gtttttttgg
     7741 tcgtgttgcc agcctatgaa attttgacga atactctgaa tatcaggtat ttttttcatc
     7801 aaccattcca tataattata accatttgga ggaaaagtac caatagctaa atggaatgtt
     7861 tcacaattca taggcatagg attatgccac caaccacgcg ggatatataa aatatctcct
     7921 gcctctaaaa tgacttccat atctactgtt gtaggcggag taatgtgcgg tatatctttt
     7981 gcttgttgca tatacaatgg catatcaaaa tttgcagcag aaagtgtcca gtgttttgtg
     8041 ccaatcaatt gaaccgcaaa cacatcacga gtatcccaat gattccgata tgatgaagaa
     8101 ctaccaaaag ctaaataacc actaacaact gtttgtgctt gagcaaattg agcaatttgt
     8161 ttagcaatac tatcaacaaa tggttcatta tcaatacggt tataaaccat tgttgcacca
     8221 tcttgcagat actgatatac ggctgtttta ttaaaacgat aacgtatctt acctacatca
     8281 ttgaatgatt caacataggc ttctttcggg attatttctc ctttgcgaaa tttgaattgc
     8341 cagtcagtag ggtctgcacg ttgatacaat tcattaattt ctttccatga gatagaacta
     8401 acatctaaag catttttaaa aataaatggc tttttatata aataattttc attaaattct
     8461 ttatattcca ttgaaaagtt aatatgcaca aataatcctt attttcatta ttaataatga
     8521 aaattaagtt tacttaacta gtgtttatat gtcaagtttt gcaatgaaaa gacgttctgt
     8581 ttttggaaga aggtattttg ttatttttag ataaagttgg atattgcttt gacattatgg
     8641 tcaaatacat tctgccgtgt gttattattt attcaataaa tctgaaacga gtttattgag
     8701 cacgacgcga atagttacac cattctcagc cgcgtatttc ttcaattctc tatgcagaga
     8761 ttcagacata tcaaaactga ttctcttgat ttcttcttgc ttgatcacat catcaataga
     8821 taagtttgct ttgtttgcac taggacgatc tgcttttaat ccgttcataa ttatttccta
     8881 caatatttaa aaatttaaaa taatatttca ttaaatatga ttatctatat ttatcaaatt
     8941 tattatctca ttcgcaagtg attgaatttc cttagctgct tctccatttg gttctttatc
     9001 cagtacagtg ctacctgtga tagcagatgt tggataaata atacgttgca caatcacggt
     9061 tgataaaaca ggtaactcat attcttctaa cgctgctcta atttctccgc caatctttgt
     9121 tcctttaata gttcggttaa ttaaaaaagc agcctttaaa tttccatcta tgagttttat
     9181 ccgctgcttg attaaattaa ccaaatcagc ggttgcccaa atatcgtatg gcgacggttg
     9241 aacagggatt aaaactaaat cagaggcttt aatcgctgat atggctaaat cggctgtttg
     9301 tggtgcgcca tcaataataa caaaatcttt ttaaacaacg ctttttaaat cacgatcaat
     9361 tgtcgggctg tcaataccaa tcacaggaac aggttgctct tcgtgaacag cagcccagtc
     9421 cctactgctt ccttgtggat cgctatcaac caataagatg ctatttcctg ctaattgcaa
     9481 ggcgcgtgcc aagtgagttg cgactgtggt tttaccactt ccaccctttt gatttaatac
     9541 ggaaataact ttcatgttag ctcctttgaa tatctacgct cattatgctt ttaattaaat
     9601 aatattttaa tctattattt aaataaataa aagaatattt acattttgtt ttaaatttta
     9661 gcaagaaaag ctgttaaaga gattactgta tcaaactaga cttttttgag atatgtggca
     9721 gaaaatgagt tgatattgcg ttaaagttta aattgagacc tttgcaaaaa agcccttccc
     9781 ccaacagccg aaacccaaac acaggttttc gtctatttcc gctcctaatt actcctgatt
     9841 ttacccaaat gcccccttaa tcctccccgg acgccccata atcaggcatc cgggccgcct
     9901 tttaggcggc agcgggcgca cttagcctgt tggccgcttt caacaggttc aaacacatcg
     9961 ccttcagatg gctttgcgca ctcactttaa tcagcccgaa ataggctgcc cgcgcatagc
    10021 ggaatttgcg gtgcagcgta ccgaagcttt gttcgaccac ataacggatc ttcgacaaat
    10081 atcggttatg tttggtttgc g
//
LOCUS       DAXHTY010000067        10096 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXHTY010000067 DAXHTY010000000
VERSION     DAXHTY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.067"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(59..253)
                     /locus_tag="ACFNMK_001471"
     CDS             complement(59..253)
                     /locus_tag="ACFNMK_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC6364720.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(243..2324)
                     /locus_tag="ACFNMK_001472"
     CDS             complement(243..2324)
                     /locus_tag="ACFNMK_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC6364721.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANHVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2800..3180
                     /locus_tag="ACFNMK_001473"
     CDS             2800..3180
                     /locus_tag="ACFNMK_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC6364722.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTADF
                     RAFSCENGLSVHVRRLDGGRIALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3463..3996)
                     /locus_tag="ACFNMK_001474"
     CDS             complement(3463..3996)
                     /locus_tag="ACFNMK_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364723.1"
                     /translation="MEKIEKFKSELLDDIFQYIQCISIFVYKKKIYYLIDYKENFELN
                     AKISFDIYLREGIITKEQYNYDYKNYRNGIWQLTKDNFESYLQSDSVIVLKKDELKEL
                     MFQGFTSAEAVRLYSVVENKLSYNDPILHMDWDRCHEDYVYDGWFAKAMDFGYLIPDE
                     FCYWKIEGRDYWKFGQL"
     gene            complement(4150..4643)
                     /locus_tag="ACFNMK_001475"
                     /pseudo
     CDS             complement(4150..4643)
                     /locus_tag="ACFNMK_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
     gene            5070..6503
                     /locus_tag="ACFNMK_001476"
     CDS             5070..6503
                     /locus_tag="ACFNMK_001476"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC6364724.1"
                     /translation="MNEYYQLIKHPDISLSPISDGIGVGNPATGEILAYVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALVVLAYEAGVPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            6612..7400
                     /locus_tag="ACFNMK_001477"
     CDS             6612..7400
                     /locus_tag="ACFNMK_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC6364725.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDQWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            7768..9324
                     /locus_tag="ACFNMK_001478"
     CDS             7768..9324
                     /locus_tag="ACFNMK_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC6364726.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVVMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESV
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVRITRLSEE"
     gene            9338..10084
                     /gene="surE"
                     /locus_tag="ACFNMK_001479"
     CDS             9338..10084
                     /gene="surE"
                     /locus_tag="ACFNMK_001479"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC6364727.1"
                     /translation="MNVLISNDDGYLSEGIDVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKTPPQSPILWNIN
                     IPAVEPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
BASE COUNT         2436 a         2507 c         2753 g         2400 t
ORIGIN      
        1 ctatattata aaaattccgt ctgaagcggc gttcagacgg aatttgtttc aagcaggctt
       61 aacagcagcg tccgccgttt gcgccgcaac ggcgcttgcg gcagtagtct tgaaattgca
      121 gcttggtcat cacgggggcg ttgggattat gtttgcgctg ctgtgcaacg tagttttcat
      181 aatcaggcac gcctgccatc aagtttgccg tcagcttgat ggttttccac caagacgcga
      241 gcttatgctt cactttgtgc ctccggctgc ttgccgtcgc ggtacaccgc cgggatttct
      301 ttggcggtcg gccagccgac tttgcgtgct ttgagggcgg tacgcaaacc gtacgcggcg
      361 acaatcacga caaccgacaa gaagaggatg gtcagaccgg cattaatctt gtcgttgaag
      421 atgatttgcg ccatttcgcc gatgtctttg gcaggcgcaa ggacttcgtt tttagccaat
      481 gcgtcgctgt atttgccggc gtgggcaagg aagctgatgc gcgggtcgct gtggaacagt
      541 ttttgcaggc cggcgtagca ggttacgaac agtacgccga cggcgggaac gagtaccacc
      601 caaacataac ggtcgcgttt catcttaatc agcaccacgg cgcacataat caaggccacg
      661 cctgccagca tttggttggc gatgccgaac aaaggccaga gcgagttgat gccgcccagc
      721 gggtcggtta cgccggtgta gagaaagtag ccccacaatg ccacggcgaa gaaggtcgca
      781 atcaggttgg cggggatgga gtcggtgttg ccgaaaggtt tgtagaagat gctgcccaag
      841 tcttgaatca taaaacgtgc gacgcgcgta ccggcatcga cggcggtcag gatgaacaag
      901 gcttcaaaca atagggcgaa gtgataccag aacgccatca tcgcctcgcc cggaatcagg
      961 cggctcataa tgtgcgccat accgactgcg agggtgggcg caccgcctgc acgggaaagg
     1021 atggtgtttt cgcccacttc cttagcggtg tgcaacaggg ttgcagcatc gacagggaat
     1081 tgcagcttgg tggtaatcac ttcggcggcg gtattggcat ccgtaccgat cagggcggca
     1141 gggctgttca tggcgaagta cacgccggga tcaagcgatg cggcggcggc aagcgccata
     1201 atggctacga aactttccat caacataccg ccgtaaccaa tcatacggac gtgggtttcg
     1261 ttttccagca ttttcggcgt ggtgccggag gaaatcagcg cgtggaagcc cgaaaccgca
     1321 ccgcaggcga tggtgatgaa gaggaacggg aacaatgcgc ctgagaatac cggacccgaa
     1381 ccgtcgataa agtgggttac ggcaggcatt tgcaaagcgg gattgacgat gacgatgccc
     1441 aaagccaagg ccgcaatcgt accgattttc aggaaggtgg agagatagtc gcgcggagtg
     1501 agcagcaacc atacgggcaa tacggaggcg acaaagccgt aaatcataat cgcccaagtg
     1561 agctggatgc cgtcaaggtc gaaccaatgt ccgatggaac ttttcgccac atcttcgccg
     1621 taaattaccg ccagcatcag caaaataaag ccgacgatgg aaatctcgcc gattttgccc
     1681 ggacggatat agcgcgtgta aatacccata aacagggcaa tcggcatagt ggcggcgatg
     1741 gtaaacgtac cccaagggct gtgaaccagt gcttttacga caatcaacgc caacaccgcc
     1801 ataatgatga ccataatcat caaaataccg atggaggcaa tcacgcctgg aacagtaccg
     1861 agttcttgtt tcacaatatc gcccaaagac ttaccgtcgc gacgcataga gacgaacaag
     1921 accatcatat cctgtaccgc gccggcaaac accacgccga agataatcca caaagtaccg
     1981 ggcaggtaac ccatttgcgc cgccaaaacc gggccgacca aagggcccgc gcccgcaatc
     2041 gcggcaaagt ggtgtccgaa caatacgcct ttgtgcgtcg gaacgtagtc caagccgtcg
     2101 ttgtggcgtt ctgccggagt caggcggtta ggatcgagcc gcattacgtg gttggcgatg
     2161 tagaggctgt aaaaacggta ggcgatgcag taaacggata tggcggcggt aaccatccat
     2221 accgcgctga cctgttcgcc tcggctgaga gccagagtgg taaaggatgc taagccgacc
     2281 agtaccacta tgccccaaat gaggaaggtt ttgagtgatt tcatcgaata aatccttatc
     2341 tcacactgtc ggaatatgcc tgaacggcgg gaaacggatg accgcctgcc ggcacgggca
     2401 caaaccggag tctgaatgat tgacgaagta tgaaacagtg cgcttgtcgg gatttgtgcc
     2461 ttatgcccgc atcaaacagt gcagggatgc gggcgcacaa aacgttaaac gccgaatagg
     2521 attttaacgc aaattagcac actgatagcg gtttttactt ggaatttgga aaaaatttac
     2581 attccttcgg gcggacaggc gggtttggac ggcatcgtca ggcaaaaggc ggcgccggaa
     2641 gacgggtaaa gaaggggcgc gcaatccgga ttattgattc atcgcgggta aattccggtt
     2701 atcgggcttg tgtgtttgcg cgtccgttta tagtatggcg ttgccgcagc ttggaatcag
     2761 ggcggttgtt tcatatctta tttttattgg ggagctttta tgaatatcag gtttttcgcg
     2821 ctgaccgtac cggttttgtc tttggcggcc tgtgccgtgc cggaggcgta tgatgacggc
     2881 ggacgagggc atatgccgcc cgttcaaaac caagccggca cggcagattt tcgggcattt
     2941 tcctgcgaga acggtttgtc tgtgcacgtc cgccgtttgg acggcggcag aatcgcgttg
     3001 cggttggacg gcaggcgtgc cgtcctctct tccgacgttg ccgcatccgg agaacgctat
     3061 accgccgaac acggtttgtt cggaaacgca accgagtggc accagaaagg cggcgaagcc
     3121 tttttcggct ttaccgatgc ctacggcaat tcggtcgaaa cttcctgccg cgcccgttaa
     3181 acggggcagc ctgtttgaac cgccgtccgg atttttcggg cggtttttta tgtcggattt
     3241 gttttgcagt tcggcctccg gcagggtttt ggctgtctga taccgtccgt aacttcgggt
     3301 attgccgagt ttgtcggcaa cgaccgctgc gacttgggcg gcgttttgcc gaacgcctgc
     3361 gttacttccc tttgtaggcc gatctctttg gcaaccgcgt ctttgtcgaa gctgtttttc
     3421 agacagcctt ttaaattcat acaatctatg gcttgcttat ctttataatt gtccaaattt
     3481 ccaataatcc cgcccttcaa ttttccaata acaaaattcg tccggtatta aatacccaaa
     3541 atccatcgcc tttgcaaacc agccgtcata aacataatct tcatgacatc tgtcccaatc
     3601 catatgtaag ataggatcat tgtacgacag cttgttctcg acaacggaat ataaccttac
     3661 cgcttcagca gaagtaaatc cctgaaacat cagttccttc aattcatcct tttttaatac
     3721 aatgactgaa tccgattgaa gatagctttc aaaattgtcc ttagttaact gccagatacc
     3781 attacggtaa tttttataat cataattata ttgttctttt gttataattc cttctcttag
     3841 ataaatatca aaagatattt ttgcatttaa ttcaaaattt tccttataat caatcaagta
     3901 atatatcttt ttcttataga caaaaataga tatgcattga atatactgaa agatgtcatc
     3961 caataattca gatttaaatt tctcaatctt ttccatattt gccaaattcc ttaataactt
     4021 tatcttcagc aagccgtaac ccacattaaa tctgcaggta aggtgtggta cgcacgccag
     4081 tgaacttgag tctaataaaa aggtcgtctg aaacattttt ccggctttca gacggccttt
     4141 tgccaaatat tatcggcagc ggctcaatgc caactttaaa cctgctccga tttcttcagg
     4201 gctgttatcc aatgataaaa ttacatcgtc tgcatcaatg gcatcccacg cttccagctt
     4261 gacatggcgg ctcgggctga ttttcaggca gccgttgtgc agccaaatat ccacgctcat
     4321 catgttttta aatagggcgc gtctggtttt atagcccaag ttcctgcata ggttggcaac
     4381 ccaatcctca tagcgttgcc gaaatttttc ggtatcaaaa aaatcttggt cttctggact
     4441 gtcataaacg aaagtcctgc tgtttgccaa cgcttgcaag accgtcgtgc ctaaagtttc
     4501 attgtcggta tccaatggca ggatatgggg ggatataggt ggtctattgc cgttaatccc
     4561 aaacctgaac atgtttgaac aatcagagct tcttcattcg tataaaaaac tgcccaataa
     4621 ttttgagcct gtttaaaaat cattatttta tctccgtaat tttgattgta tagtggatta
     4681 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg caaggcgagg
     4741 caacgctgta ctggtttttg ttaatccact ataatgtttg gatttttgcc atactctacc
     4801 acacgttgca actgcaatct ttgttcctta tatattatta aagggctgca ttacttttct
     4861 ttctaaatta ccattgtata aaaagtattt ttcttaaacg acagggattg cgcccgcgcc
     4921 atattcagac accgcccgga tgttgcgctg cccgatcgga tgcttcagac ggcatcggaa
     4981 gggtttgcag ttttggaata tgaagatgat aatgtccgcg agattgacgg catttgaaag
     5041 cggttattcc gcataccgga ggacacgaaa tgaacgaata ttaccaatta atcaagcatc
     5101 ccgatatttc cctttccccg atttcagacg gcatcggggt cggcaatccg gcgacgggtg
     5161 aaattttggc gtatgtccgc aagacggatt cggacaagct gaaaaacctg attcaaaaag
     5221 cagctgcggc acaaaaatta tgggcggcga aaaccgcttt ggaacgcgcc gacatattgt
     5281 ggcgttggta ttttttagtt aaagaaaaca aggaagcact ggcgcgcctg atgacgatgg
     5341 agcagggcaa aagcctgacc gagtcgcgcg gcgaaatcga ttatgcggct tcgtttgtgc
     5401 gctggtttgc ggaagaggcg cggcggattg acggcgatgt gctgacaagt gtgaaagcgt
     5461 cgcaaaaact ggtcgtgttg aaacagcctg tcggcgttac cgccgcgatt acgccgtgga
     5521 acttcccatc tgcaatgatt gcgcgcaagg ctgcgcctgc tttggcggtg ggctgcgcga
     5581 tgatcgtcaa acccgcatcg ctcacgcctt tgagtgcgta tgcgttggtc gtgctggctt
     5641 atgaagcggg cgtaccacag gatttgttgc ccgtcgtcag cggtcgcgca tcggaaatcg
     5701 gccgtgaatt tgccacgaac ccgattatcc gcaaaatcag cttcaccggc tctaccgaag
     5761 tcggtgcgaa aatttttgcc gacagcgcgg cggacattaa aaaactcagt ttggagctgg
     5821 gcggcaacgc gccgtttatc gtgtttgacg atgccgattt ggacaaagcc gtcgaaggtg
     5881 cgctcgccag caagttccgc aacagcggtc agacctgcgt ctgcaccaac cgcgtttacg
     5941 cccaatcggg catttacgac gcattttgcc gcaaattgag tgaaaaagca gccgcgctca
     6001 aattgggcaa cggcttggag gatggcgtaa accaagggcc gctgattgag gaaaaagcgg
     6061 tagaaaaagt cgagcagcac atcgccgacg cgctctcaaa aggcgcggtc tgtctgaccg
     6121 gcggcaaacg cagcgcgttg ggcggaacgt tttttgagcc gaccgtctta agcggcgtaa
     6181 cggcgcaaat ggcggtagct cgcgaggaaa ccttcgggcc tttgtgtccg gtattccgtt
     6241 ttgaaaccga agccgaggtc atcgaggctg cgaacaatac ggaatacggt ttggcagctt
     6301 accttttcac cgccgacacc gcacgccagt ggcgcgtcgg cgaagccttg gaatacggta
     6361 tggtcggcat caatacgggc ttaatcagta atgaagcggc accgttcggc ggcgtgaaac
     6421 gcagcggttt gggacgtgaa ggcagcaaat acggtgcgga cgaatatctg gaattgaaat
     6481 atctgtgtat ggatgtcggg tgacggatgc cgtctgaacg gcagggtttc agacggcgcc
     6541 gcattttaag cagtctctat ctgttgtaca atgcgccctg tttttacggt tatttttgat
     6601 ttgaacaaga tatgatggaa aacggaaaaa cagtcccgag atggcgtttt gccttgaaaa
     6661 gtgcgggctg gcatctctta atcagcctgt cggttgcagg gctggcggca ttgctggttt
     6721 ttaaggtttg gtatccttat ccttatgccg agctgacggg agggctgtcg ctttatcagc
     6781 tggtggtggc tgtcgatatt gtatgtggtc cgctgctgac tttaattttg gcaagcccga
     6841 agaaaaagac aaaggcacgc atggtcgatt tttccatggt cggcatcatc cagctggcgg
     6901 ctttggtgta cggtctgcac agcgtttctt tggcgcgtcc cgtggtggag gcgtttgaaa
     6961 aagacaggat taatgttgtg accgccgccg aagtggcggt tgaagatttg cacaaagcgc
     7021 cggaagggct gcaaagcctg tcgtggttcg gcatccgccg cattgcattg aaagaacctg
     7081 aggatgcgga tgagaagaac aagacgctgg atttgtccct gaaaggtatc gagccgagta
     7141 tgcgtcccga ccagtggctg ccgtattccg acaaggaagc agaagaaatc cgcaaacatc
     7201 tgaaaccgct gaaagtcttg gcggatgcga gaaaaacgac ggttgcggac attctgaaac
     7261 aggcaggtct cgccgaaggg gaggagctgt attacctgcc gtttaccagc agcaggcaga
     7321 aagagtggat agtcattacc gataaagagg gcaacaccaa aggctacgcg ccgatagacg
     7381 gcttcatcat caccccttaa gcgttgggac tccgtccgca ctcgaacatc cgttcttcgc
     7441 ggcggtagaa tcagactgta tttgagaggg gcgcaacccc tcaatcaggg caacacatag
     7501 gggaaggctt tatgtgccgt ccgtgtgttg aaacatttca aaataagctc atgccgtccg
     7561 aacatccttt cagacggtat ggcgttttgc cattgccccg ataagctgtt aaactattta
     7621 aattatttcg accgaaggta cacgcacatg caagaccccg agcagagcag caagcccgcc
     7681 cgccgttttt tgtgcgtacc gtctgttcag gcagcttgag cgcttcaggc tgccttttcg
     7741 cgtttgaact tgaggaatac gaaaatcatg gatttcagtt ggttggcaga accgcatacc
     7801 tggataggtt ttgccacgct tttggtgttg gaagtcgtat tggggataga caatcttgtc
     7861 tttgtggcga ttttggcaaa caaggtccag cccgcacggc gcgaccgcgc acggattatc
     7921 gggctggggc tggcagtcgt catccgcatc attatgcttg cttttatggc gcacatcatc
     7981 acgctgaccg agccgctgtt ccaaatcggc ggcctcgccg tttccggcaa ggacatgatt
     8041 atgctcgcgg gcggtatttt cctgctttac aaagccacca ccgaactgca cgaacgcctc
     8101 gaagggcaca accggtttac cgttgccgac agccagaaaa aacacgcgcc gttttggggc
     8161 gtggtcgcgc aaatcctgat actggatgct gtgttttcca tcgattcggt catcaccgcc
     8221 gtagtgatgg tcgatcacat cgttgtggcg atgggcgcgg tcgtcgtcgc gatggcggtg
     8281 atgatttccg ccagcaaact cttgaccgaa tttgtagaca ggcatcccac cgtcgtgatg
     8341 ctctgccttg gttttttgtt gatgatcggt ttcagcctga ttgccgaagc cttccatttc
     8401 cacattccca aaggctacct ctacgccgcc atcggcttct cgattttaat cgaactgttt
     8461 aaccagattt cccagcgcaa cagccgcaaa aacgactaca tcggcagctc gtggcgcaag
     8521 cgcaccgccg aaaacgtctt gggtatgatg ggtatacgcg aaagcgtgct tgccgacgcg
     8581 ggcggcgaat ccgtggatga cgcgcatttt gaagaaaacg aaaaatcgat gatacgcagc
     8641 gtgctgacgc ttgccgaacg cccgattatg ggggtaatga tcccacgccg cgacatcgaa
     8701 cggctggaca tttcccaaag ccgcgaagaa cagtgtgccc aactgcaaaa cacgccttac
     8761 agccgcctgc tcgttgtcgg taaggcgggc gtggacgaac ctttgggcta catcaacaaa
     8821 aaagacctgc tgtcccaact gctggaaaca ggcggtctcg acattcagac ggcattgcgc
     8881 cagccgctcg ttttgcccga cagcaccacc gcgctgggcg caatcgaact cttccgccaa
     8941 agcagcgcgg attatgcttt ggtggtggac gagttcggcg cggtattggg catggtaacc
     9001 atgaaagacc tgctcgaaac catcgcaggc gagttccccg aagaatttga gcgcgaagaa
     9061 gaaccagccg ttcaggggaa tcccgatgaa agcctgacgg tggaaggcgc gttggaatat
     9121 gtggaactcg caccgcaact caacctgccg cagcaggagg aagatgccga tttccatacg
     9181 gttgccgggc tgattatgga agaattgcaa accatccccg atgtcggtga ttttgccgat
     9241 ttccacggct ggcggtttga agtggtcgaa aaagaagggc agcgcatcga acgggtcagg
     9301 attaccaggc tgtccgaaga atgattcagg ataagaaatg aatgttttga tttccaacga
     9361 cgatggctat ctttccgaag gtatcgacgt tttggcgcgc gttacggcgg aatttgccaa
     9421 cgtcagggtg gttgcgcccg aacgcgacag gagcggggtc agcaattcgc tgacgctgga
     9481 acgccctttg cagttgaaac aggcgcaaaa cgggttctac tatgtcaacg gcacgccgac
     9541 cgactgtatc cacatcgggc agtctgtatt ttcggatttt caggccgatt ttgtcttttc
     9601 gggcatcaac cggggcgcga atatggggga cgacacgctt tattcgggga cggttgcggc
     9661 ggcaaccgaa gcctacctta tgggcatacc cgctgtggcg ttttccttaa acgacgcttc
     9721 cggacgctat tgggcaaccg cagaaaaggc actgtggaca ttgttggcgc attttttcaa
     9781 aacccccccg cagtccccta ttttgtggaa catcaatatc cccgccgtcg agccggaaga
     9841 tgtgcggggc attaaaatcg cccgtttggg caggaggcat cacggtcaga acgtcatccc
     9901 cgcgcgcaat ccgcgcggcg aacagattta ttggataggc ccggtaggca gggtttccga
     9961 caaagaagag ggaacggatt tcggtgaatg cggcgcaggt ttcattaccg taacgccgct
    10021 gcaaatcgac ctgaccgcct atccggacat ggcggaaaca gcggcgttct ggcatgcgga
    10081 ctgaccgttt catcaa
//
LOCUS       DAXHTY010000068         9909 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXHTY010000068 DAXHTY010000000
VERSION     DAXHTY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9909
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.068"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..1028
                     /locus_tag="ACFNMK_001480"
     CDS             <1..1028
                     /locus_tag="ACFNMK_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798991.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC6364728.1"
                     /translation="GIGDILYGTRYAIDKAAMRNIAPLPAEGKFAAIGGLGSVAGFEK
                     NTREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKAAVSGDFSKSYTCSF
                     HGSTLVKTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTARYGNPYQETVYIEISDG
                     IGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITLKQQPLKAYNL
                     TVADWHTYFVKGNQAETEGVWVHNDCPYGEKYRTEVGSYTNTHESGRTYSGKGTRQRS
                     QISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNNYNRIESPGKK
                     YRKQDGD"
     gene            1031..1354
                     /locus_tag="ACFNMK_001481"
     CDS             1031..1354
                     /locus_tag="ACFNMK_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC6364729.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1413..1640
                     /locus_tag="ACFNMK_001482"
     CDS             1413..1640
                     /locus_tag="ACFNMK_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364730.1"
                     /translation="MEIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            1767..2309
                     /locus_tag="ACFNMK_001483"
     CDS             1767..2309
                     /locus_tag="ACFNMK_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC6364731.1"
                     /translation="MINHIEKYIGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LGLSKHDLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVVYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVGNLSDMNRKPFTW"
     gene            2443..3111
                     /locus_tag="ACFNMK_001484"
     CDS             2443..3111
                     /locus_tag="ACFNMK_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC6364732.1"
                     /translation="MVKTADGYKAIARIRVGDRVFAKDEASGVTGYKPVTAQYGNPYQ
                     ETIYIKVSDDIGNSQTLISNRIHPFYSDGKWVKAEDLKAGNRLSSESGAEQTVQSITV
                     KPEPLQAYNLTVADWHTYFVKGNQAETEGVWVHNSCPPKRAPEYHAGTVSESNFLNAA
                     EKWLGENYKSYPNGRYVSQDGMRQVRYGYHETNSSTHHGHFESYDKPNGRVIENSAVT
                     IIRD"
     gene            3115..3387
                     /locus_tag="ACFNMK_001485"
     CDS             3115..3387
                     /locus_tag="ACFNMK_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364733.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPMGILPKPVALSIVQYEN
                     DPGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            3508..4053
                     /locus_tag="ACFNMK_001486"
     CDS             3508..4053
                     /locus_tag="ACFNMK_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC6364734.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLNNEVDNLSDMKRKPFVW"
     gene            4218..4817
                     /locus_tag="ACFNMK_001487"
     CDS             4218..4817
                     /locus_tag="ACFNMK_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC6364735.1"
                     /translation="MAHIRVGESVLSKDEASGKTGYKPVTAQYGNPYQETVYIKVSDG
                     IGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITFKQQPLKAYNL
                     TVADWHTYFVKGSQAETEGVWVHNACPPKRTGSSKNEKHGDGGRSQISAESRIAELKN
                     KIIPGMSKNERLKIERTIRNITKNANRKAKGEEHGRRGR"
     gene            4798..5254
                     /locus_tag="ACFNMK_001488"
                     /pseudo
     CDS             4798..5254
                     /locus_tag="ACFNMK_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5475..5833
                     /locus_tag="ACFNMK_001489"
                     /pseudo
     CDS             5475..5833
                     /locus_tag="ACFNMK_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5845..6180
                     /locus_tag="ACFNMK_001490"
     CDS             5845..6180
                     /locus_tag="ACFNMK_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364736.1"
                     /translation="MMADKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
     gene            6329..6823
                     /locus_tag="ACFNMK_001491"
                     /pseudo
     CDS             6329..6823
                     /locus_tag="ACFNMK_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6943..8076
                     /locus_tag="ACFNMK_001492"
     CDS             6943..8076
                     /locus_tag="ACFNMK_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364737.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGTADDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHEEEVWLNYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGYGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLENLEKEVRRYAEAAARRSGGRRDLSY"
     gene            complement(8163..8438)
                     /locus_tag="ACFNMK_001493"
     CDS             complement(8163..8438)
                     /locus_tag="ACFNMK_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364738.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLMCISVFWIVVFNL"
     gene            complement(8455..9270)
                     /locus_tag="ACFNMK_001494"
     CDS             complement(8455..9270)
                     /locus_tag="ACFNMK_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6364739.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNNRINQTEED
                     NYSTGVGSCINGFPVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(9574..>9909)
                     /locus_tag="ACFNMK_001495"
     CDS             complement(9574..>9909)
                     /locus_tag="ACFNMK_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1301 family transposase"
                     /protein_id="HFC6364740.1"
                     /translation="GGNVHDSQVANDLIEVIQEAQYFIADKGYDSQEIRDKAIEHGMK
                     AIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLKQFRSIATRYEKLARNFKSMLYLA
                     CSIIHAKLN"
BASE COUNT         3084 a         1887 c         2150 g         2788 t
ORIGIN      
        1 tgggcatagg cgacatactg tacggaacgc gctatgccat agacaaagcc gcaatgcgca
       61 acatcgcccc cttacccgcc gagggcaaat tcgccgccat cggcggcttg ggcagcgtgg
      121 cgggctttga aaagaatacg cgtgaagccg ttgaccggtg gatacaggaa aaccccaatg
      181 ccgccgaaac cgtcgaagcc gtcttcaacg ttgccgcagc agccaaagtc gcgaagctgg
      241 caaaggcggc gaaaccgggg aaagctgcgg ttagcgggga tttctcaaaa tcctacacct
      301 gctccttcca cggcagtacc ttggtcaaaa cggcagacgg ctacaaagcc attgcccata
      361 ttcaagccgg cgaccgcgtc ttcgccaaag atgaaaccag tggaaaaact ggatacaaac
      421 ctgttaccgc ccgatacggc aatccgtacc aggaaaccgt ttacattgaa atttcagacg
      481 gcatcggtaa cagccaaacc ctgatttcca accgcatcca tccgttttac agtcaaggaa
      541 aatggataca ggcaggtcgt ctgaaaaaag gcgacaccct gctttccgaa agcggtgcaa
      601 aacagacggt tcaaaacatt actctcaaac agcagccgct caaagcctac aacctgaccg
      661 ttgccgactg gcatacttat ttcgtcaagg gtaatcaggc ggaaacggaa ggggtttggg
      721 ttcataatga ttgtccgtat ggagaaaaat ataggacaga agttggttct tatactaata
      781 ctcatgaaag tggtcgaact tattctggaa agggcacaag acaacgttcc caaatctctg
      841 gagcaagaga agctagggtt aataatgacc ctcatatagc tacagatttt actcctgcca
      901 aaaataatcg agaggcattc aaagatgaat ctagaagaat tgacgctcac ggtggttcca
      961 aaagtaccaa taattacaat cgaattgaaa gcccggggaa aaaataccga aaacaagatg
     1021 gagattaaaa atgattgttg acgaaaaatc tacttcagat gtactgaaac aatatcaaga
     1081 actagggttt gatttgacaa aacctatgat aatagagttt tttataggag gttctcaaaa
     1141 gaatctgcaa tcaatagaaa gccgactcat ttcttatcgg caagattttc aaatcagtat
     1201 tgaacaagat gaattcggag aaatgtggac ttgctattgt tccgtcaata tcgtcccttc
     1261 ccttgaaaac attttagcta ttgaaactac gttatttggt attgcacaaa aaaatgattg
     1321 taattatgaa ggttttggtt cgtatgggaa ctaacaaaaa gtgtaggttg ggttgaaaaa
     1381 actatattcc cattaaagat taggggatgt ctatggaaat aaaattcaat actttggggg
     1441 ttatcctgaa tggggtcaat cctgaagaaa aattcataaa aattattgat gaccaggaaa
     1501 ataccggtgg atttctgata ctgttatctt caaatgacaa attttcatcg tttgacagtt
     1561 acgatgactg ggtcgaaaac ttagaaatat taaaagaata cctgcaagaa tctcattgga
     1621 taataaagtg ggtcggatag tcggcaaacg tagtccgtct gaaacgcttt tacttttcag
     1681 acggactttc tatcaatggg aacaaggcgt tttgtctcaa tctggtctat gattattcga
     1741 tacagtgatg ggaagctaag aaaaatatga ttaatcatat tgaaaaatat ataggcacta
     1801 tttctcatgg ctcgaaaagc gattcgggaa gtcagtacaa attaaatatc gcagctattc
     1861 cctcttcccc taacagggat ttaaaaactt atattacttt agggttaagc aaacatgatc
     1921 tgaattataa aagcagattt gaaatacttt tcgtatgttc tttaaaatat gatgaaaatc
     1981 agattttccc atttttaaga tggttggcag aaaccatcat tgagaacaaa aaaatccttc
     2041 ttcgggggca ggttgtttat ttgcccagaa gcattgttga ttcaacaaaa atggatgcac
     2101 tgtatgtttc cgccccattt tatttcgacg atgattttca ggtctgttat ggcgaacact
     2161 acaatatcgt tttccctttg cttgtcccct tgtataaaca ggaagccgaa ttggtggaaa
     2221 agaaaggttg gaatgctttt gagcagttct tgttggataa tgaagttggc aacctttcgg
     2281 atatgaatcg gaaaccgttt acttggtaag gattttctta aatttgatta aaggtcgtct
     2341 gaaaccgtgt ttaactttca gacaaccttt ccttcatttg aaacaggata ttgaaaacta
     2401 agtttttcaa cattctacac ctgctccttc cacggcaaca ccttggttaa aacggcagac
     2461 ggctacaaag ctattgcccg tatccgggtc ggcgaccgtg tcttcgccaa ggacgaggca
     2521 agcggagtaa cgggatacaa acctgttacc gcccaatacg gcaatccgta tcaggaaacc
     2581 atttacatta aagtttcaga cgacatcggc aacagccaaa ccctgatttc caaccgcatc
     2641 cacccatttt attcggacgg caaatgggtt aaagcagagg atttgaaagc gggaaacagg
     2701 ttgtcgtctg aaagcggcgc ggaacagacg gttcaaagca ttaccgtcaa accggaaccg
     2761 ctgcaagcct acaatctgac cgttgccgat tggcatacct acttcgtcaa gggcaatcag
     2821 gcggaaacgg aaggggtgtg ggttcataat tcttgtcctc ccaaaagagc tcccgaatac
     2881 catgcaggaa ctgtatctga atctaatttt ttaaatgcag ctgagaaatg gctaggggaa
     2941 aactataagt catatccaaa tggtcgatat gtatcacaag atggaatgcg gcaggtacga
     3001 tatggttatc atgaaacaaa ttcatctacg catcatggtc attttgaatc atatgacaaa
     3061 cctaatggac gtgtaataga aaatagtgcg gtaacaatta ttcgggattg aagaatgatg
     3121 agtgtaaaag aattattcaa agtcatctta gatgaaaata aagattttcc tattagaacg
     3181 attcatcgta ccccgatggg aatacttccc aaacctgttg ctttaagtat tgttcaatat
     3241 gaaaatgacc cgggatttta tttgttttat ttggatgaga ccggtcagga acaaacggat
     3301 acttatcatg acacattaga ttcggcattt gaacaggctg aatttgaatt tggaatcagc
     3361 aaagaggaat ggatgcaaag tccttagacg gcaaaatcaa gatgccgctt tatttttcag
     3421 acggcatttc cgtcaataaa aaagtcggta ttggagccca aaacagccta cgtcttgccg
     3481 ggatagggat gaaatcttta agaaaaaatg attatagttg aacatttaga acattatttg
     3541 ggtgaaatcg aaagcggtat aaaatgcttg gatcgacgtt atcatctcag cgtttccgtc
     3601 ttcccatcac aaccatataa aggggtaact actttttcta ctttaggctt gaatcgatat
     3661 gacttgaact ataagagtag gtttgaatta atttttacct gttctgaaga atggaataaa
     3721 gaaaatattg cagcttttct atcaggagtg gctgaatatt tgattgacaa tagacaacct
     3781 attttaagag gtgaaattat tcaattaccc agggtaatta tcgaaggtag caaaatggat
     3841 gcactgtatg tttccgcccc attttatttc gacgatgatt ttcaggtctg ttatggcgaa
     3901 cactacaata tcgttttccc tttgcttgtc ccgttgtata aacaggaagc agaattggta
     3961 gaaaagaaag gttggaatgc ttttgagcag ttcttgctga ataatgaagt tgacaacctt
     4021 tcggatatga agcggaaacc gtttgtttgg taagggcttt cttaaatttg attaaaggtc
     4081 gtctgaaacc gtgtttaact ttcagacaac ctttccttca tttgaaacag gatattggga
     4141 actaagttct tcaaaaatcc tgtctccgtc ccttatacgt cggtgcttta gtcaaaacgg
     4201 cagacggcta caaagctatt gcccatatcc gagtcggcga gagcgtcctt tccaaggacg
     4261 aggcaagcgg gaaaacagga tacaaacccg ttaccgccca atacggtaat ccgtatcaag
     4321 aaaccgttta cattaaagtt tcagacggca tcggcaacag ccaaaccctg atttccaacc
     4381 gcatccatcc gttttacagt caaggaaaat ggatacaggc aggtcgtctg aaaaaaggcg
     4441 acaccctgct ttccgaaagc ggcgcaaaac agacggttca aaacattacc ttcaaacagc
     4501 agccgctcaa agcctacaat ctgaccgttg ccgattggca tacttacttc gtcaagggca
     4561 gtcaggcgga aacggaaggg gtttgggttc ataatgcgtg tccgcctaaa aggacaggaa
     4621 gctccaagaa tgaaaaacat ggagatggcg gtcgaagtca aatatcagca gaatcacgaa
     4681 ttgctgaatt aaaaaataaa attattcccg gaatgtccaa aaatgaacga ttaaagattg
     4741 agcgaacaat cagaaatatt acaaaaaatg ccaatcgaaa agcaaaagga gaagagcatg
     4801 gtcgacgcgg tcgttaaaac tcccgaattt ttacctttta cttctgtagg aatattccgt
     4861 tttggtgcgg atataacaga gtacaaaaac atactggaaa cttttatgta tgaacctcct
     4921 gatgagttta gaacagaata ttatgaatct cccgactcaa atttactcat ttccgtcaaa
     4981 aaaaaacaaa attatttcga tattttgtta tcaggagcta tattttatgg gcgttaatat
     5041 aataggtctg aattttgaag attttaaaca acttttccat caccctaaat catacggtgt
     5101 tgataaatat tatttatcaa atgaatctta tccgacatat gtctatgagt ttgatgaaat
     5161 aggagtacaa gcttgggaag caaaagggaa aattgttact atcattgccg ggggcaaaga
     5221 caattattcg acagaacctt actacgatga ataacattaa tggcaggcat aggttgggtt
     5281 gagaactcaa caacaagagg tcgtctgaaa ccgtaatcga tttttcagac gacctttctg
     5341 tcaatccaaa cagattgtcg aatcttaatc caacctgtgc ttgctgtacg gaacaggcac
     5401 ggtgttcatt cacaagttaa gtcatatatt gaatcaaaag gcggcattgt taaaacaggt
     5461 ttaggagatt aaacatggca tcatatgttt ccataaaagg atggattgaa tgctctgacg
     5521 atgatataaa aatcatccaa gaaaacatta ataatttttg gaataattgt cccttcaata
     5581 tcgaagagaa agaatctgca aaaatatata aaagtgggtg ggttttcccg acaaatagtt
     5641 ttaattggag cagctatatt tttttggtgc atgcgtaaaa tcttatttca ttatttattt
     5701 tgaaaaatgt attaaaacaa taatggaatt ggatattgaa atatcaggtt tttttgaatt
     5761 agattattat gaagatagtt ataaagtaaa ttggaaaata aataatggca atttaataca
     5821 aacgactaat taaatggaca aaaaatgatg gctgataagg aacaagtaga aaagatgatt
     5881 tattctgaaa ataaacagag tgttattgac ggtatgctcg gtatgacctt cagctcggag
     5941 gaggatgaaa tcccttggat ttctgagaaa ttgacggagt tgtcgaagca taaagacttg
     6001 gatattgcca gattgtcatt aacctgtttc ggacatcttg ccaggatgca tgaaaatatc
     6061 ggcgattgcg ataaagtgat tgctttattg cgctcaaaac aaggtaatcc ggattttcaa
     6121 ggttttgccg aagatgcgtt agacgatatt tccttcttta tatttaaaaa gcgtccgtaa
     6181 gcaccggcaa ggtcaaatct ggcaaatgcc gtctgaagcg gtattcggct ttcagacggc
     6241 attttctccg attggcacag gatgcagggg cttgccggct tgattttgcc gaagttgttg
     6301 gcaaacaatt tggagggggc ggcaatttat ggaaaataga tttgcaaaaa tcgttaagga
     6361 tatttttagt tttacccaat ttgtttccga agcggaaaat aaaattccta accaatcctc
     6421 ccgttattac gactgctggt aggaattgga aatcttgaat gcttccgcat tggaagattg
     6481 ggaaatatcg gaaaagcctg aaaaatggga gaaatggaca gaaaaatatc agaccgaagc
     6541 gttacagtta gtcaagaatc tatttgccga agaagttcct gaaaataata tagtttgcga
     6601 tatttctcat tttttgcatt ttgccaatcg ggcagaagcc gacattccca atcaatcacg
     6661 tttatatgcc gaatattggt gggaaatgga aacgttcaaa atgtcggttt tagaagattg
     6721 ggaacgctcg ggaaagccgt tagattggga aaaatggcaa aggcaatatc aatttgaagc
     6781 atttgaatta gtcaaaaaat tgaatcaaag caaacaagag taacaagctg cagcctgcat
     6841 aggggttttt aaagtacggc atcaaccgta gattaaatta aaaatcaaac aatcaaagct
     6901 cgtctgatat aatcgatata ctctgttaaa cgaaaaataa atatgtttaa aaaattcaaa
     6961 ccggtactgt tgtcattttt tgcacttgta tttgcctttt ggctgggaac aggtattgcc
     7021 tatgagatta atccgcgttg gtttttgagc gatacggcaa ctgaagtacc tgaaaatccg
     7081 aatgcttttg tggcgaaact tgcccgcctg ttccgaaatg ccgacagggc ggttgtcatc
     7141 gtgaaggaat cgatgaggac ggaggaaagt cttgccggaa ctgcggatga cggtccgttg
     7201 cagtcggaga aggattatct cgcgctcgct atccggctca gtcgtttgaa agaaaaggcg
     7261 aaatggtttc acgtaacgga gcaggaacat gaggaagagg tctggctgaa ttaccatatc
     7321 ggcgagggcg gtttggttgc ggtttcgctt tcgcaacgct cgccggaagc gtttgttaat
     7381 gccgaatatc tgtatcggaa cgatcgtccg ttttctgtaa atgtgtacgg cggaacggct
     7441 cacggagaaa attatgaaac gacaggagaa tatcgggttg tttggcaacc ggacggttcg
     7501 gtatttgatg cgtcggggta cgggaaaatc ggggaagatg tttatgagca ttgcctcggg
     7561 tgttatcaga tggcccaggt atatttggcg aaataccggg atgtcgcgaa tgatgagcag
     7621 aaggtttggg acttccgcga agagagcaac cggattgcgt cggactcgcg cgattctgtg
     7681 ttttatcaga atatgcggga attgatgccc cgggggatga aggcgaacag tcttgtggtc
     7741 ggctatgatg cggacggtct gccgcagaaa gtctattgga gtttcgacaa tggaaaaaaa
     7801 cgccagagtt tcgaatatta tttgaaaaac ggaaatcttt ttattgcaca atcttcgacg
     7861 gtagcattga aagcggatgg cgtaacggcg gatatacaga cctatcatgc gcaacagacg
     7921 tggtatttgg atggcgggcg gattatccgc gaagagaaac agggagacag actgcctgat
     7981 tttcctttga acttggaaaa tttggaaaaa gaggtgcgcc gttatgcaga ggctgcggcg
     8041 agacgttcgg gcggcaggcg cgacctttct tactgaacgc caatgccgtc tgaaaccggt
     8101 tttcagacgg cattggagag tcaaacgctt tttgcaatgc tgtatattcc aaatccaatt
     8161 cgctataaat tgaaaaccac aatccaaaaa acagaaatac acattaaacg ggtcttttta
     8221 ccgaccgtat catcccatag gttttccaca ccttttttca tccataaatt caaaccattt
     8281 aagtttgcat ccttaccgac aattgcagca gcatcatccg cataacggtc acccaaatta
     8341 gccaaataac gccaaccgcc ataggtatcg ccacggaaag cataaccacg caaggtttgg
     8401 atttgttctg gtgttaaaac aacttcaccc tcattcataa taagatcctt taaattattt
     8461 tttacttgaa ttgtttacaa aactctgtac caattgctct acttgttggt gataacctac
     8521 caactcctgc tgtgtgagct gctgcttttc ccgaataaac gggtaatacc gtaccgggaa
     8581 accgttaata caggaaccta cgccagtgct gtaattatct tcttcagttt ggtttattcg
     8641 attattccga tgagtaataa tcgccggatt ttctcctttt ttaatatagt aaaattcatg
     8701 aaaatatgga ttttcaggaa tatgtctttt atcttcataa gcctcgtatt caggataggc
     8761 agatggcatt tttttaaccc cgtaagtaag caatctttct cccatagtcg tgcttaaact
     8821 acacgcatct tttcggataa caatatcgtc cacgctatcc aaaccgtcgc gtagaaattg
     8881 gataaagtgt gctttgtttt gctggttgta tggctcaaga acccaacctt ggtcggtttt
     8941 tttgtaatag acgctaatct ctatcaaatt aatggagtaa gcatcctgcc cgggcgtgtc
     9001 ggaaagccat tttgtggcat tgtcttgcgt ataccgtgca agatagccat aggggataac
     9061 cattttggtg ccctgaaaat caaatgtaac cgtatgttca aatcctgtca ttggctcggg
     9121 attgttgaaa ctggtttgtt ctttagcctg ttgcgcactt actgcttttt gatctgtctt
     9181 ttcattcaca ggttcacatg aggtcatagt aaaaacactc ccaataaaca aaataaaaag
     9241 ttgataaatg gtagtagcat atggtctcat aatttcaagc ctagaaatta gttaaagaat
     9301 atattatggt agatactaac tctttaagtt gacatattgt gcgatatcga gggctgtacc
     9361 gtattttccc atcgtatttt cccatcgtat tttcccatcg tattttccca tcgtattttc
     9421 ccatcgtatt ttcccatcgt attttcccat cgtattttcc catcgtattt tcccgttctt
     9481 tatatgagcg gcaggcattc tcataaaaga agggataccg ataaggaaaa atacctcggt
     9541 ataaaaaata tactgattga ttagggcgtg tcctcaattt aacttagcat gaataataga
     9601 gcaagccaag taaagcatcg atttaaaatt acgagctaat ttctcatagc gagtagcaat
     9661 actacgaaac tgctttaatc gtgcaaatgc attctcgact aagtggcgta atttataaag
     9721 gtagctatca aaatcagggt taggtttctt ggcattttta cgctttggta taatagcttt
     9781 cataccgtgt tctatcgctt tatctctgat ttcttgcgag tcataccctt tgtcagcgat
     9841 aaaatactgg gcttcttgta tgacttctat caagtcgttt gcaacttgac tgtcgtgcac
     9901 gttaccccc
//
LOCUS       DAXHTY010000069         9824 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXHTY010000069 DAXHTY010000000
VERSION     DAXHTY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9824
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.069"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(40..1335)
                     /locus_tag="ACFNMK_001496"
     CDS             complement(40..1335)
                     /locus_tag="ACFNMK_001496"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6364741.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGAQIFEQSPMTCIEP
                     HQNGWLVYTPEGSVECKNVVYAVNTYAGLNPIFKPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1461..2348)
                     /locus_tag="ACFNMK_001497"
     CDS             complement(1461..2348)
                     /locus_tag="ACFNMK_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6364742.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2348..3313)
                     /locus_tag="ACFNMK_001498"
     CDS             complement(2348..3313)
                     /locus_tag="ACFNMK_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6364743.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3329..4453)
                     /gene="potA"
                     /locus_tag="ACFNMK_001499"
     CDS             complement(3329..4453)
                     /gene="potA"
                     /locus_tag="ACFNMK_001499"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC6364744.1"
                     /translation="MTATTASSTKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4648..4755)
                     /locus_tag="ACFNMK_001500"
                     /pseudo
     CDS             complement(4648..4755)
                     /locus_tag="ACFNMK_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4834..5103)
                     /gene="rpsO"
                     /locus_tag="ACFNMK_001501"
     CDS             complement(4834..5103)
                     /gene="rpsO"
                     /locus_tag="ACFNMK_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC6364745.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5326..6261)
                     /gene="secF"
                     /locus_tag="ACFNMK_001502"
     CDS             complement(5326..6261)
                     /gene="secF"
                     /locus_tag="ACFNMK_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC6364746.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6265..8118)
                     /gene="secD"
                     /locus_tag="ACFNMK_001503"
     CDS             complement(6265..8118)
                     /gene="secD"
                     /locus_tag="ACFNMK_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC6364747.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8330..8596)
                     /gene="yajC"
                     /locus_tag="ACFNMK_001504"
     CDS             complement(8330..8596)
                     /gene="yajC"
                     /locus_tag="ACFNMK_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC6364748.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAISAKVD"
     gene            8724..>9824
                     /locus_tag="ACFNMK_001505"
     CDS             8724..>9824
                     /locus_tag="ACFNMK_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC6364749.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACTGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALTHTSKYLNRLESRLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2520 a         2600 c         2434 g         2270 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatactgc ctgcgacgct taacggctgt ctttccactg
       61 ataatatttc gagccgagga aagaaccgag tttgcgcaaa aacggttgca ggataatatt
      121 cggctggcgc aaccgctcaa acggcttcag acggcattcg tcccctaaaa ttgcttcggc
      181 aaccgccaaa cctgcaatac ctgttatcgc catcccgtgt ccggaataac cttgcgtata
      241 aaaaacattc ggggccaaac gtccgaaatg cgggacaagg ttggcggtaa tatcgcactc
      301 cccgccccac gaatattcga ttttgacatc ggcaagctgc ggaaaaactt taagcatatc
      361 ttggcggaca agctcggtca tacgcgcagg attgtcgata aactcgttat ccttaccgcc
      421 gaaaagcagt ctgccgtccg cgctgaggcg gtaataatcc aaaatatggc ggttgtcgca
      481 tacagccata ttgttgcgga taagcccttt tgcgcgcgcc cccaagggtt cggtggcaat
      541 aataaaggtg ctaacggcaa tcgccttacg ctccaaaggc ttgaatatcg gattcaaacc
      601 ggcataggta ttgacagcat agaccacatt tttgcactcg acgctgcctt cgggcgtgta
      661 aaccagccaa ccgttttgat gcggttcgat gcacgtcatc ggggattgct cgaaaatctg
      721 cgcaccggct tcggcagcgg cacgagcgat gcccaaagtg taattgagcg gatgcaggtg
      781 tccggataag gggtcgaatt gtgccccttg gtacatatcg ctgtcaagct gctgtttcaa
      841 ctcggcttta tcccaaagtt gataatgact cgcaccgtaa tgccgttggg cgtgttcatg
      901 ccactgctgc aactcttccc aatgctgcgg acggacggca accgtggcat aaccgcgctg
      961 ccaatcgcaa tcaatggcat gtttgcggac gcgttcgtcc accagttcga ccgcctgcaa
     1021 agactgttgc caaaaccatt gcgcctgctc caagccgacc tgtttttcaa tttcccccat
     1081 accgcaggcg taatcgctga taacctgccc gccactccgt cccgacgcgc cgaaaccgat
     1141 acgcgcggct tccaacacaa ccgtttcatg tccctgctcc gccaagggca atgcagtgca
     1201 caaaccactc aatccgccgc cgatgataca ggtatcggtt ttcagacggc attgaagttt
     1261 cggataaaca gtatgaggat taaccgaact aaaataataa gaaggcagat attcttgaaa
     1321 atcagggcga atcattgtgt ttgctttatc aggtgtattt tcggacggaa tgatacaggc
     1381 tgtcgggcca tatcgtccaa acagaaaatc ggttgaagaa aacaggctga cccagtcatg
     1441 cggtcagcct gccttattaa ttaatttgct ttctcggcag ccaatttttc ttggcggtag
     1501 gcttctgccg cttctcggtc acgcttggtt gcctgcctca tcatccaata attgacgatg
     1561 atgaccaatg ttccgatgat gccgattagg atggtcgcca agacattcat ctgcggatca
     1621 agacccagct tgattttgga gaaaatcacc tgcggcaatg tggatgaacc ggggccggag
     1681 aggaatgagg taattaccaa atcatccaaa gacagggtaa tgccgagcag aaagcctgaa
     1741 gcgatggcag gggcaatcaa aggcaaagtg atgacaaaaa agattttcag cggacgcgcg
     1801 cccaaatcca ttgcggcttc ttcgagcgat tggtcaagct caaccagacg cgaacggata
     1861 acaacggtaa tgtatgccat gcacagcgtc gtatgtccta agaaaatagt gaaaaagcca
     1921 cgatcgaagt agagatgttg taaccattcg ctgccctgca aaaatatctg tacctgaata
     1981 atcagcagca gcatagacag accggtaatc acgtcgggca tcaccatagg tgcggaaatc
     2041 ataccggcga acaaggtact gccgcgaaaa cgtttaatcc gcgccatcgc atagcctgcc
     2101 agcgtgccca aaacgacggc agcaagcgaa gacacaacgg caatccgcag cgacagccaa
     2161 gcggcttcca agatggtgtc gttttccagc aatgcgccgt accacttggt cgaaaagccg
     2221 ccccaaacgg ttaccagctt ggattcgtta aacgaataga tgaccaaaac aaccagcggg
     2281 atatacagaa acgccagcga cagtgccaac atcagtttca agaaccaaga taatttggat
     2341 ttctgcatta tttggctcct tcttccaatt cgcggttttc ataatgctga aacagggcaa
     2401 tcggcacgac cagcagcgcg accatcacga cggcgacggc ggaagccagc ggccagttgt
     2461 tttgatcgaa gaacgcctgc cacaagactt taccaatcat caggttttcc gaaccgccga
     2521 ccagctcggg aatgacgaac tcgccgacag cagggacgaa aaccagcatg gagcctgcaa
     2581 taatgccggt tttcgacaaa ggcagggtaa tcgtcaagaa cgatttgacc ggccccgcac
     2641 ccaaatcgga agccgcttca agcaggcggt tgtcgagttt caccagttgc gtgtatagcg
     2701 gcagaatcat aaacggcaga taggcgtaaa ccatcaccaa attgagcgaa aaggcattgt
     2761 agaacaaatc caaaggctcg ctgataatac ccattttaat caacaggttg tttacaatac
     2821 cgttatgccc gagcagaccc atccacgcat agacgcgcaa caggaacgat gtccaaaagg
     2881 gcagcataat ggcaagcagc aaaccattgc ggacagaagg attggcacga gaaatcgcat
     2941 aggcggtcgg ataaccgacc aacaggcaaa tcactgtcgt catcaatgcg gttttgattg
     3001 aagaccagta ggtcatcaga tagatattgc tgttttcact gtcgccgaac gggttaaggg
     3061 tatgccaaaa attttggaaa atatcggcat agttttgata actgatggcg atattcagac
     3121 gacctaaatc ttcgtcgatg ctggtcagcg gcgtaaacgg cggaatggcg atttcttgtt
     3181 cggcaaagct gattttcagc acgatggcga acggaatcag aaacagcacc aaaagccaaa
     3241 tatacggtac ggcaatcacc gcacgctgcc ccggacggcg gaacagtttg tttttcagtt
     3301 tattaaggtt cattgcattc cccttaaatc aacggaacaa cggagtcggt tggttttccg
     3361 gccagctgat atagacggtt tcgtcccaag tcggcggtgt aatgttgcgc acataccagt
     3421 aaggggcggg gacttggctt ttgacgacgc gcccgttgcc gagcttgata tggtaaatgg
     3481 cgaagctgcc caaataggcg atttctttta ccgtgccttt cgcccagttg tagtcgccca
     3541 aatattcggg tttttcttta tataaatcaa tatcctctgg tcgaatacta acccaaaggt
     3601 cctgctcgct cggaccaccc aaaccgtgat cgatgcggac gtggttttcc aaaccttcgc
     3661 attcgataac ggcatagtcg gcatgatctt caatcaccac accgtcaaag atgttggttt
     3721 cgccgataaa ctcggcagtg aagcggctgt tgggatagtc gtacacgtcg ctgggtgtgc
     3781 cgacttgctg caactgaccg tcagacataa tggcgatgcg ggtcgccatc gtcatcgcct
     3841 cttcttggtc gtgcgtaacc ataatacagg ttacgccgac ttgttccagc gtattgacca
     3901 actcaagctg ggtttgttgg cgcagttttt tgtccaatgc accgaggggc tcatccagca
     3961 gtagaatttt cggacgtttt gccagactgc gtgccaaagc aatgcgctgc tgctgaccgc
     4021 cggacaattg gtgcggtttg cgtttagcaa atttggtcat ctgaaccagg cggagcattt
     4081 cttcgacgcg cgcggcgatt tcgcctttag gcattttgtc ctgtttcaga ccgaaggcaa
     4141 tgttttgttc tacggtcata tgcggaaaaa gcgcgtaact ttggaacatc atattgatgg
     4201 ggcgatcata gggtgcaagt ttggtaatat cctgaccatc aaggataatt tttccctgat
     4261 tgggactttc catacccgcc agcatacgca gcagtgtaga ttttccgctg ccggaactgc
     4321 ccaaaagggc gaagatttcg tgttgataaa tgtccaagtc gatgttatcg acagcgtaat
     4381 tgtcaccaaa ctttttcacc aaaccttgga tttgcagata aggcttggtt gaagacgcag
     4441 tggttgcggt cataatggca atactccaat aaaaagacga gtaccggcaa aacggatgtt
     4501 cgaatgggtg ataaaaagct gtttgattgc tggcgggagt taaacgtttg atgccgtctg
     4561 aaactcttgt aaagcgcacg ggcagcatga aatggaacaa gattccaaag aactttatat
     4621 tatattagtt tatgcggttt tcgggcaata tagtggatta aatttaaacc agtacagcgt
     4681 tgccttgcct tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgatttaa
     4741 atttaatcca ctataaatat ggatttgagc ttgtcggaaa gcaacagaaa agaaaacacc
     4801 gcccattttt ctgggcggtg tcggaaagcg taattattta cgcagaccca agcgggtaat
     4861 caacgcgcga tacgtatcgg gctgggtacg gcgcaagtag gccagcaggc ggcggcgttg
     4921 gctgaccatt ttcaacaggc cgcgacggct gtggtggtct ttggggttgg ctttgaagtg
     4981 gggggtcagg tcgttgatgc ggaaagtcaa cagagcgact tgtacttcgg aagagccggt
     5041 gtcgccttct ttgcgttgga aatctttaac gatttgtgct ttttgttcta cggtcagtgc
     5101 cataatgaaa actccaaaaa tataagaatc cccataggga ttcagacaag tttgccaagc
     5161 ctgaagacaa cggcaaactc cctatgccca agataggaca acgtggcatt atgacacaat
     5221 tcccgcattt ctgcacaata ttttaagacc gtggaagtaa ccgttaaaaa tgccgtctga
     5281 agcattgtct gcttgagacg gcaatgttca gacggcatat gcgcttcaaa ccacgatttc
     5341 ttctttcttc ttcggttctt taccgatatt gtcgcggctc aaaccgaaca ttagcagaag
     5401 cgggctggca accaatacgg aagaataaat gccgaacacg atgccaatgg tcaacgccat
     5461 agaaaagccg tgcaaggccg caccgccgaa caccagcatg gatacgacca tcgcctcggt
     5521 cgaaccgtgg gtaatgatgg tgcggctcat cgttgcggta atcgcgttgt cgatgacttc
     5581 cggcacggca tgtccgcgca tcgccggctt gcggaagttt tcacggatac ggtcgaagac
     5641 gacgacggat tcgttcacag aatagcccaa tacggcaagg atacccgcca agacggtcag
     5701 cgaaaattcc cattggaaga aggcaaagca gccgagaata atcacgatgt cgtgcatatt
     5761 ggcgataatg gcagatacgg caaaacgcca ttcaaaacgc atcgacaggt aaataatgat
     5821 gccgataacg acaaaaccta aagccatcaa tccattactt accaattcct caccgacttg
     5881 cgggccgata aattcgactt ggcgcaaggt aacgtcggga ctgtcttttt tcagcaaatc
     5941 cataacctga ttggacaact gtgcggaagt aacaccttct ttgttcggca ggcggatcat
     6001 gatgtgtttg ttcgtaccca atgcctgaac ctgtacatca cctattttca gcgtatcgag
     6061 gcgttcgcgc atcttattga catccgcacc ctgctgatat tggacttcca ttaccgtacc
     6121 gccggtaaat tcgacagaga aattcagacc tctggtaacc aaaaagaaca cggcagcgat
     6181 aaacgtaacc aacgaaatga aggtcgtcag tttgccgtag ctcataaacg gaatatcgcg
     6241 tttgatttta aagagttcca tagcttactc cttacctgcc gcagtttcgg cttcaggttt
     6301 ccataccgca ccgatggaaa tattctgcaa tttgcgtctg cgtccgtacc acagattgac
     6361 caacgcacgg aatacgacga cggaggaata catcgaagtc agaataccca aacagtgtac
     6421 gaccgcaaaa ccgcgtactg ggccggaacc gaataccaaa agcgcgatac cggcaatcag
     6481 cgaagtcagg ttggaatcga caatggtcgc ccatgcgtgt tggaaaccga gattgattgc
     6541 ctgctgcggc ggcacgccgg cacgcaattc ttcgcggata cgttcgttaa tcaagacgtt
     6601 ggagtcgatt gccataccca aagtcaacgc cagcgcggcc atacccggta acgtcaacgt
     6661 tgcctgcatg gcagacaaaa taccgattag gaacagtatg ttggcactca atgcaatggt
     6721 agaaaagaaa cccatcagac gatagtaaac caccatgaat gcagcaacga tggcaaaacc
     6781 ccataaagtc gaatggaagc ctttttcgat gttctcctta cccaaagacg gaccgatggt
     6841 acgttcttcg acaatctgca tcggcgcggc aagagaaccg gcacgcaaca gcaaagacgt
     6901 atcattggct tcggctgtcg tcatgcttcc ggaaatttcc acgcgtccgc cggtaatggc
     6961 agtacggata accggcgcgg ttacaacctc ggattttcct tggtcgatca aaaccatcgc
     7021 catgcgtttg ccgacatttg cggcagtcag ttcgccaaaa atgctgccac ccgcgctgtc
     7081 caagctcaga ctgacggcag gtgcgcccat ttggtcgaaa ctcggttgcg catcgttgat
     7141 gttgtcgccc gtcagctcga cctgtttgct gattagcaga gtttcgggac gatctccgcc
     7201 gcttgaaagc agctcataac cgctcggcac gttgccttcc aatgcctcgc gcaacttggc
     7261 aggatcgtcc tccaccatac gcaattccaa agtcgcggta cggccgatga tgtcttttgc
     7321 cttggcagta tcctgaacgc ccggaagctg cacgacgata cggtcggcac cggactgctg
     7381 gatgacgggt tcggccacgc ccaactcgtt cacacggttg tgcagggtag tgatgttctg
     7441 tttgaccgca tcggaacgca ctttattgac cgcctcttcc gaaagcgtca agacgatatt
     7501 gctgccgtct gaattcagcg ttgcttcagg aaacagcttg cgcaactgcg gcagagcctt
     7561 ttgcacatca cctgcatcct gcaaagggac ggtcaggctg tttccagcct gacgcaccgt
     7621 gccgctgcgg attttttcgc ggcgcagttc gcggcggatg tcgcccgaat aacgttcaaa
     7681 cgttttctgc atcgccgctt tcatatcgac ctgcatggtg aaatgcacgc cgccgcgcaa
     7741 gtccaaaccc aaaaacatcg gattggcttt gattttcgcc atccattcgg ggctgtccgc
     7801 caacaggttg agcgcggtaa tatacccttc gcccaaagtg ttttcgatga cgtcgcgcgc
     7861 tttaagctgc gtttctgtgt ctttgaaacg cactttcagt gaattgtcca caacaaacat
     7921 cccgtcggtc tgaatacctg cgtttttcag cgcggcatcc actttgaatt gagtctgttc
     7981 gttgatgatg atggcttgtc ggttggtcga tacctgcacg gcgggtgttt cgccgaatag
     8041 gttgggcagc gaatacactg cggcaaccgc aattgtgaac acaatcagca gatatttcca
     8101 taaaggataa cggttcatca ttgttcctta atggttggaa ccccaccctt tcggtggtgt
     8161 cggaatcggg ctatttcaga agaggcaaaa acccttccca gccaggcaag accggaaggc
     8221 ggcatcttga atatgccgcc ctgcgtgtcg gaacatggtc aagccttcgg ttggaattca
     8281 aaacaaagtg ccgcattcgg gcttttccga tgcggcttgt cggcacaaat caatcgactt
     8341 ttgcagaaat cgcattgcgt tccacttcga cctcgatttt tgtaccctgt ccgatatcca
     8401 cggtaaaaaa ctgttcgccg actctggtta ccttaccctt gaaacctgcc gccaagacca
     8461 ctttgtcgcc gactttcaag gcggcaagca ttgcctgatg cgctttgaat ttcttttgct
     8521 gcggacgcat gatcaggaag tagaacacca ccataatcaa cactaaagga gcaaattgtg
     8581 caacagcttg attcataatt tatccgttct ttctaatatg gttgaaaatc gagaggggta
     8641 tataataaca taagaccgta aacaatatat cgggtttgcc gtccgtaccg accgtatacc
     8701 gcagcctgcc cgtccacaaa cccatgtcct tgacccgcct aatcttgaaa ttctatgcgc
     8761 tgttgcgcct ctttttgggc aaaaacgccc gcaccgcatg gatttcgcat cccgcctgca
     8821 ccgggcacga acccggcgca aaccatcccg attcgcccga ccgcatcctc tgcatcgagc
     8881 aggcattgcg ccgcgccggt atttggcagc acctccaaac catagaggcg gaagaaatca
     8941 gcgatacgcg cctcgcgctt acccatacga gcaaatattt aaaccggtta gagtcccgcc
     9001 tgcccgaaga cggcaagatt tgccgcttgg acgacgacac cgttatcagc aaaaactccc
     9061 tgtctgccgc ccgtttttcc gcaggttcgg cagttcaggc agtcgacatg gtcatgaacc
     9121 gtaaagcatg gcatgccttt tgcgccgccc gcccgcccgg acaccatgcg ggcagcggca
     9181 aagccggcgg attctgcctg ctgaacaacg ttgccgccgg cgtcatgcat gccattgccg
     9241 aataccgcct gaaacgcatt gccgtcatcg atttcgatgt ccactacggc gacggtacgg
     9301 cagaaatatt caaagacgat ccgcgcatcc tgtttttcaa cctgtttgaa accgaccttt
     9361 ttcctttccc cgaaaacaac gatatgcccg acggcggcaa tatggtgcac ctgcccttgc
     9421 cgccgggaac gggcagccgc acattccgcg aagccgtccg cagacagtgg ctgccccggc
     9481 ttgccgcatt caaacccgaa ctggtgctgc tgtcggcagg attcgacgca caccgtctag
     9541 acgaatcggg caggctcaac ctgcacgagg cggattttgc ctggctgaca cacaaaatca
     9601 ttcagacggc atcgggctgc cccggcaaaa tcgtatccgt actcgaaggc ggctacaccc
     9661 ttgaacctct ggcacaatct gctgccgagc atatccgcgt gctggcaggg ctgggcaaat
     9721 ccgatgccgc aaccgcctat cagaaaacac tggacccaac aaaaaaacgg tttgccaaac
     9781 cgaaaaccgg acaggtgcgc caacctaccc aatccgaccg ttac
//
LOCUS       DAXHTY010000070         9744 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXHTY010000070 DAXHTY010000000
VERSION     DAXHTY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9744
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.070"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(615..1151)
                     /locus_tag="ACFNMK_001506"
     CDS             complement(615..1151)
                     /locus_tag="ACFNMK_001506"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC6364750.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVASEDMAACALRELAEETPYTTD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(1185..1541)
                     /gene="folB"
                     /locus_tag="ACFNMK_001507"
     CDS             complement(1185..1541)
                     /gene="folB"
                     /locus_tag="ACFNMK_001507"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC6364751.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1597..2199
                     /gene="plsY"
                     /locus_tag="ACFNMK_001508"
     CDS             1597..2199
                     /gene="plsY"
                     /locus_tag="ACFNMK_001508"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC6364752.1"
                     /translation="MFNISAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            2287..3132
                     /locus_tag="ACFNMK_001509"
     CDS             2287..3132
                     /locus_tag="ACFNMK_001509"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC6364753.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     AVLIDPPYEEKQDYWRVTETLKSALKRFESGCYLIWYPCLSREESRKLPEELKKLVPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3378..4352
                     /locus_tag="ACFNMK_001510"
     CDS             3378..4352
                     /locus_tag="ACFNMK_001510"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC6364754.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYILYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(4511..4729)
                     /locus_tag="ACFNMK_001511"
     CDS             complement(4511..4729)
                     /locus_tag="ACFNMK_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC6364755.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            5073..5335
                     /locus_tag="ACFNMK_001512"
                     /pseudo
     CDS             5073..5335
                     /locus_tag="ACFNMK_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            5503..7278
                     /gene="ggt"
                     /locus_tag="ACFNMK_001513"
     CDS             5503..7278
                     /gene="ggt"
                     /locus_tag="ACFNMK_001513"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFC6364756.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLIEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            7341..7604
                     /locus_tag="ACFNMK_001514"
     CDS             7341..7604
                     /locus_tag="ACFNMK_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364757.1"
                     /translation="MSNQTKIQAIKQASEQIFAICETPNTALQAIHLILRHGGAGELS
                     WQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLPLGSLVLT"
     gene            7759..9009
                     /gene="glyA"
                     /locus_tag="ACFNMK_001515"
     CDS             7759..9009
                     /gene="glyA"
                     /locus_tag="ACFNMK_001515"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC6364758.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            9137..>9744
                     /locus_tag="ACFNMK_001516"
     CDS             9137..>9744
                     /locus_tag="ACFNMK_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6364759.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELIIPDYS
                     TLCRYRNWLAQDDTLSELLKLINCQLTEKGLKIEKASAAVVDATIIQTETFAKFLSLP
                     TAETQTQVFGCFRPKYLLILPKHPLNPPRIPDNQASGLPFRR"
BASE COUNT         2611 a         2636 c         2120 g         2377 t
ORIGIN      
        1 aagtatccat atattctatc tttaattatt tcattatttt cttcattttc ttcatctaaa
       61 aatatacatc tttttaatag ttccccatta aattcataaa catcgtaata agctttaaaa
      121 atagatatca tatcatttcc ttatcttcca taaatcggct tcggttgttt aaataccgcc
      181 cgtgccaatt accgttctca aattcaaatt gggcttttag gtctataaca aagaaaaagc
      241 cttggcagga taaacctacc aaggcttttt attaaaaaaa acgctctatt actgtgtcta
      301 aaaatgcaaa aaaagtaacc atcataaccc ttttgccaaa atcaatatca tcttccgatg
      361 aatgaattga cggtatataa gaaagaatta ataagctcat aaaccggtaa gaaaaatcag
      421 taacaaagat tttcattatt aaccattctt aaaaatgatc acgttcgttt gggtaattat
      481 tatacccttt tccctcttat tactaaagca cgaatgactt taaaggacgc gcccagagac
      541 aagccgaaaa ggcatatctc atccgccaaa caaaatgccc gccaatctac tccggagggc
      601 agttcaacac cctgtcaatc cttcatcagc cagtattgca aaccgattaa tgtcttgccg
      661 tctttaattt catcgtttgc caatgcctga cggacttctt ctttcgacat caatacggtt
      721 tccgtaatct cgtcttcgtc attggcaagc gtactgccca aacgcacgcc ttccgcctcg
      781 aacagataca ttttttcgtt gcaaaaaccg accgccgtat aaaaactgta aagcaggcgt
      841 acgctgtcgg tggtataagg cgtttcttca gccagttctc gtaaagcgca tgctgccata
      901 tcctcgctcg ccacatccag cttgcccgca ggaagttcca atgtcgcctg atttgccgca
      961 taacgccact gccgcaccaa aaccactttc ccctcgtccg taaccgccaa cacgcatgcc
     1021 gcacccggat ggcggatgac gatacgctgc ccttcattgc cgttgggcaa cctgacctta
     1081 tccctgctga tactgacgaa accgccctcg taaatggttt cgccgcctaa ttttacttcc
     1141 ctcaaatcca cttcatatct cctttccgtt tcctattctg ccgttcaatc ttcacgcttg
     1201 ccgcgctcga tttccacgcc aacctcgcgc acgccttcca aaatacccgg cttgacgatt
     1261 ttcacgcgca cccacaccgc gccgaaatat cccaaaacca aatcggcaat atattccgcc
     1321 aacgcttcca aaagcaggaa atcctgttct ttcagatgtc ggcgcagcgt ttcgcatacc
     1381 tcggcataat gcaccgtatt ggcaatatcg tcgtccgaac ccgctttctc gggaacaccg
     1441 atgtccaaat cgacaatcag ggtctgcaac cgttcgcgct cccagccgta cacgccgata
     1501 agcgtatccg ccttcatacc gtgcaaaaag attttatcca taatccgccc cgttttttgc
     1561 taaaataacc gcaccattct aagtaaagta ccgcaaatgt tcaacatatc ggctgttgcc
     1621 gtttcctatc tgatcggctc actttctttc gccgtcatcg tgtccaaata ttacggcatg
     1681 gacgacccgc gcacctacgg atcgggcaat ccgggggcaa ccaatgtttt acgcagcggc
     1741 aaaaaaaagg cggccgcgct gacgctcttg ggcgatgccg ccaaaggttt agttgccgtt
     1801 ttgcttgcac gcgtgcttca agaaccgctc ggtttatccg acagcgcaat cgcggccgtc
     1861 gcactcgccg cgctggtcgg gcatatgtgg ccggtgtttt tcggatttaa aggcggcaaa
     1921 ggcgtggcaa cggcattggg cgtgcttctg gcactctctc ctgcaactgc cttggtctgc
     1981 gcgttgattt ggcttgtgat ggcattcggc ttcaaagtat cctcccttgc cgcattaacc
     2041 gccacaatcg ccgcccccct tgccgcactg ttttttatgc cgcatacttc ttggattttc
     2101 gcaaccctcg caatcgccat attggtgttg ctccgccata agagcaacat cctcaacctg
     2161 attaaaggca aagaaagcaa aatcggtgaa aaacgctgat tcggcatttg ttttccatta
     2221 cgtcatgccg tctgaaacca tgttcccgcg ttcagacagc atttccgcac aaacggcttt
     2281 cataccatgc tcagttacag acacgcattc cacgccggca accacgccga tatgctcaaa
     2341 cactttacct tgtttttagt gctgcaatat ttcaaccgca aagacaagcc ctactggtac
     2401 atcgacacgc acggcggcgc gggtgtgtac aatctcgaag gcagcgaggc gcagaaagtc
     2461 ggcgaatacc ggcaaggcat cgccctgctc cgacaggcgc aaaacctgcc tgccgaactc
     2521 tctgactttg ccgcgcacat acaaaaaatc ctgccctcgc ccgaacttta ctgcggttct
     2581 ccgtggctgg cacaatcgct gacccgcgtc ggcgacaaat tacgcctgtt tgagctgcac
     2641 cccaccgact ttgtccatct gcaaaacaat atgggcgaag caggattggg caaacgcgga
     2701 caagtgttgc gcgaagacgg ttacaaaggg ctgatttccc tgctgccgcc cccgccgcgc
     2761 cgtgccgccg tcctcatcga cccgccttac gaggaaaaac aggactactg gcgtgtaacg
     2821 gaaacgctga aatccgcttt aaaacggttt gagtccggct gttatctcat ctggtatccc
     2881 tgcctcagcc gcgaagaaag ccgcaagctg cccgaagaac tgaaaaaact cgttcccgac
     2941 aactaccttc acgccgaact gcacgtccac gcgccgaaag ccgacggttt cggtatgcac
     3001 ggcagcggaa tgttcgtcat caatccgccc tatctgttgg cggaacaatt ggcggcaaac
     3061 ctccccgccc tgacgcggct gctggcgcaa gacgaaggcg cgcgctacct tttggacagc
     3121 aaaatccgct gaccccgatg ccgtctgaaa cccttcagac aacatctccc gtcaaataaa
     3181 caaaaccgga cgatataccg gcagtcggca cttttaagaa taaatatccc ctcatccgcc
     3241 tgccgcgttc cgacttgccc catgccgttt ttttacacta caatccccta gactatcctg
     3301 caaaacgcac caaaggaata cgccacaata acctgccgcc tttcagacgg cacaaacctt
     3361 tccgaaacga gcaaactatg gacacactga cccgattcct ccccgaacac ctgcaacaaa
     3421 accagctgcc cgaagcactc ggcggcgttt tactgtccgt tgtttccgcc tgcaccgaaa
     3481 tcaacgccaa agtccgcctc ggcgcactgg ccggcgtatt gggtatggcg ggcacgggca
     3541 atattcaggg cgaagaccaa aaaaaactgg atgttatcgc caacaacatc atgattgaca
     3601 cactcaaagc caaccctgcc gttgccggtt tggcaagcga ggaagaagac tctttcgtaa
     3661 gtgccggtga aaacggacgc tatctcgtcc tattcgaccc tttagacgga tcgtccaata
     3721 ttgatgtcaa catttccgtc ggtacaattt tctccatcct tgaaaaaccc gaaggcgcat
     3781 tggcaaccga atcattcctt caaacgggca gacagcagct tgccgcaggc tatatcctct
     3841 acgggcctca aacccagctc gtattcacat tcggacacgg cgtatatatg ttcacgctca
     3901 atgccgaaaa cgaatttgtg ctgaccaaag aaaaaccgaa agtacccgaa agtaccaaag
     3961 aatttgccat caatatgtcc aaccgccgcc actggctgcc ccccgtccaa caatacatcg
     4021 acgagctctt ggcgggcgaa accggcacgc gcggcaaaaa ctacaatatg cgctgggtag
     4081 ccagcatggt tgccgaaatc caccgcatac tgatgcgcgg gggcgtgttc atgtatccgc
     4141 aagacaaacg cgacccgtcc aaacccggca aactgcgcct gatgtatgaa gccaatccta
     4201 tggccctgat actcgaacaa gccggcgcat cggcaagcaa cgcccgtcag gacatactcg
     4261 gcatccgtcc cgaaagcctg caccagcgcg tcgccgtcat tatgggcagc agcgaagaag
     4321 tggattacct caaccgcctg cattcaaaat aaaaggcagc ctgaccgata aagaaaggcg
     4381 gcagcgcgca tccgacgctg ccgccgatat ttcgtttata gccttaaaaa gatagaaaac
     4441 cacctttccg aacggcaaaa atgccgtctg aaaagttttc agacggcatt ttaaaaatta
     4501 aagcccgaat ttatttgccc gctgcttttt gcaggatggc gtacaactcg tctttcagtt
     4561 tcaatttggc ttttttcagc tcatcgatgg tttccgcacc gctggtaacc ggattgttga
     4621 ccagaccggt aattttatcg tccagctcgt tgtgttcgtc gaacagacgg gcgaagcggg
     4681 aattttcctg tttcaatttg gaaatcaaat cacggtattc tgggaacata tcgctttcct
     4741 ctcttggttt acggttaaaa agtcaaactg aatctttaca ttaattataa caaagataat
     4801 atttttatcc agtgttttat cggatacggc aaaattttct tttaacaccg ccgtccggtt
     4861 gataaaaacg ttgacaatat cacgctttac gcataaattt tcagcctttc ctactgtata
     4921 gtggattaac aaaaatcagg gcaaggcgac gaagccgcag acagtacaaa tagtacggaa
     4981 ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgagaca
     5041 acgccgtact ggtttttgtt aatccactat atatgaaagg cacgccccag tttccccaat
     5101 gtggattttc agcgcgtgca gtagaggtgc tgacccaatt ggcagaccct ttgcttttgt
     5161 taatatccta gaaaacccag aaattcgcag tactctacca ctcatcgcca atcggccaac
     5221 ctttccacag ctttgggtta atggtgagtt aatcggtggt agtgatatta tcctacaaat
     5281 gtaccaatca ggtgagctaa aaccactcat tgaagagcat agcccaaagg tctgattact
     5341 taatatctta aaataaatat caaaaaagac tgctgatgac attagcggtc tttttttgat
     5401 ggtcaagatt gatagtttgg attgaattat ttagccatat taaatacaat aatcagcatg
     5461 ccttgtatga atcatcaatc aaactcaggc gaaggagtgc ttgtggctaa aacatattta
     5521 ttgactgcat tgataatgtc tatgacaatc tctggatgtc aagtcatcca tgccaatcaa
     5581 ggtaaggtta atactcattc tgctgtcatc acaggtgcag acgctcacac gcctgaacat
     5641 gcaacgggac tgatcgaaca aaagcaggtg attgcaagtg attttatggt agcgtcagcc
     5701 aatccattag caacacaagc tggctatgat atcttaaagc aaggcggtag cgctgcagat
     5761 gcgatggtgg cggtgcagac gacactaagc ttggtagagc cacagtcgtc aggcttgggc
     5821 ggtggtgcat ttgtgttgta ttgggataat accgccaaaa cattgaccac atttgatggg
     5881 cgtgagacgg caccgatgcg tgcgacgccg gaattatttt tggataaaga tggtcaacca
     5941 ttgaaattta tggaagcggt ggtcggtggt cgctcggtgg gtacgcctgc tatccctaaa
     6001 ctgatggaga caatacatca gcgatacggt gtattgcctt ggggaaaatt atttgataca
     6061 ccgatccgct tggcaaaaca aggctttgag gtgtcgccaa ggcttgccat ctcggttgag
     6121 caaaatcagc agcatttggc acgctatcca aaaacagccg cttatttttt gccgaatggt
     6181 gtgccgctac aagcaggcag cttgctgaaa aatttagaat ttgctgacag tgttcaggcg
     6241 ttagcagctc aaggtgcaaa agctctgcat actggtaaat atgcccaaaa tatcgtttca
     6301 gttgtccaaa atgctaagga taaccccggt caattatcct tacaagattt atctgattat
     6361 caagtggtgg aacgcccgcc tgtttgtgtg acttatcgta tttatgaagt atgcggtatg
     6421 ggtgcaccaa gctcaggtgg gattgctgtg ggtcaaattt tggggatttt aaatgaattt
     6481 tcacccaatc aggtggggta tgatgctgaa ggtttacgtc tcttgggcga cgcttctagg
     6541 cttgcgtttg cggatcgtga tgtatatttg ggcgaccctg attttgtacc agtacccatt
     6601 cgccagttga tttctaaaga ctacctaaaa catcgcagcc agctgcttga gcagtcggat
     6661 aaggcattgc ctagcgtatc tgcaggcgat tttattcatg agtgggtgtc ctcacaagcg
     6721 attgagttac cttctaccag tcatatatca attgtggata aggcgggcaa tgtgctatca
     6781 atgacgactt ccattgaaaa tgcgtttggc tcaactttga tggcaaatgg ctacctgctg
     6841 aataatgagc tgacagactt tagttttgag ccaataaagc agggcaaaca ggtcgccaat
     6901 cgtgtcgagc ccggcaagag accacgctcc tcaatggcac caaccatcgt atttaaagca
     6961 ggcaagcctt atatggcaat cggctcccca ggtggtagcc gtatcattgg ctatgtcgct
     7021 aagacgattg tggcacatag tgactggaat atggatatcc aagatgccat cagtgcaccc
     7081 aatttattaa atcgctttgg tagctatgaa ctagaaacgg gtacaaccgc cctccagtgg
     7141 caacaagcat taaatgattt gggctataag actgacgtgc gtgagctaaa ttcaggtgtt
     7201 caagccatta ttatcgagcc aagcaggctg gttggtggtg ccgatccacg ccgagaaggt
     7261 cgagtgatgg gtgattagag gtgaagcttg tattttgtga atttgaccgc attaagttaa
     7321 tattaaaata gggggaaatt gtgagtaatc aaacaaaaat acaagccatc aagcaagctt
     7381 ctgagcagat cttcgcaatt tgtgagacgc caaacacagc attacaagcc attcatctga
     7441 ttttaagaca tggtggggca ggagagttgt cttggcaagt ggtgtatcag cgggttatgg
     7501 ctgataaaga tgtggttggt gctggttatt tgattgattt tgcacaaact gccgaaaatt
     7561 taccatttga tgtattgcct ttgggtagcc ttgtactaac ataaaggtaa tgagacgcta
     7621 aaaaccagca tgttaaataa attacctgat aatgccaaag aaaatttacg cattatggac
     7681 tatctgcctt aggttttgaa gtattttttt atacaaaaaa cactcatgat accctgatta
     7741 cacaaaggag ccgagaacat gttttcaaaa agcgttaccc tcgcacaata tgaccccgat
     7801 ttggcagcag ccattgccca agaagaccaa cgccagcaag accacgtcga gctgattgcc
     7861 tctgaaaact acgtcagctg cgccgtaatg gaagcgcaag gttcccagct gaccaacaaa
     7921 tacgccgaag gctaccccgg caaacgctac tacggcggct gcgaatacgt cgatattgtc
     7981 gaacaattgg cgattgaccg cgtcaaaaaa ctgttcggcg cgcaatacgc caacgtccaa
     8041 ccgcactccg gctcgcaagc caaccaagcc gtgtacgctt ccgttttaaa accaggcgac
     8101 accattttgg gtatgtctct agcgcacggc ggccacctga cccacggcgc gagcgttaat
     8161 atttccggca aactctacaa cgccgttact tatggcttgg atgaaaacga agtgctcgat
     8221 tatgccgaag tggaacgctt ggctctcgaa cacaaaccta aaatgattgt ggccggtgcg
     8281 tctgcctacg cgttgcaaat cgactgggca aaattccgcg aaatcgccga taaagtcggc
     8341 gcatacctct ttgtcgatat ggcgcactat gccggtctgg ttgccggcgg cgaatatccc
     8401 aatcccgtgc cgttctgcga cttcgttacc accaccaccc acaaaaccct gcgcggtcct
     8461 cgcggcggcg taattttgtg ccgcgacaat acccacgaaa aagcgttgaa ctcttccatc
     8521 ttcccaagcc tgcaaggcgg tccgctgatg cacgtcatcg ccgccaaagc cgtagcgttt
     8581 aaagaagcgt tgcaacccga gttcaaacaa tacgcaaaac aagtgaaaat caatgctgcc
     8641 gccatggcgg aagaactggt caaacgcggt ttgcgcatcg tttccggccg caccgaaagc
     8701 cacgttttcc tcgtcgacct gcaaccgatg aaaatcaccg gcaaagccgc cgaagccgct
     8761 ttgggcaaag cgcacatcac cgtcaacaaa aacgccattc cgaacgatcc ggaaaaaccc
     8821 ttcgttacct ccggcatccg catcggttcc gccgccatga ccacacgcgg ttttaacgaa
     8881 gccgacgcgc gcgtattggc gaacttggtt gccgacgtat tgtctaaccc cgaagacgaa
     8941 gccaacctcg ccaaagtccg cgaacaagta actgctttgt gcaataaata ccccgtttac
     9001 ggcgcgtaag cctttttaaa aatattccgc caagcaatcc aatgccgcct gaaatctcat
     9061 aatgtttcag gcggaaacct ttgcaaaatc ccccaaaatc tcctaaattc ccaccaagac
     9121 atttaggaga tttctcatga gcaccttctt ccggcaaacc gcacaagcca tgattgccaa
     9181 acacatcgac cgtttcccgc tattgaagtt ggaccaggtg attgattggc agccgatcga
     9241 acaatacctg aaccgtcaaa aaaaccgtta ccttagagac caccgcggcc gtcccgccta
     9301 ccctctgctg tccatgttca aagccgtcct gctcggacaa tggcacagcc tctccgatcc
     9361 cgaactcgaa cacagcctca tcacccgcat cgatttcaac ctgttttgcc gtttcgacga
     9421 actgatcatc cccgattaca gtaccttatg ccgctaccgc aactggctgg cgcaagacga
     9481 caccctgtcc gaattactca aactgattaa ctgccaactg accgaaaaag gcctaaaaat
     9541 agagaaagca tccgccgccg tcgttgacgc caccattatt cagaccgaga cctttgcaaa
     9601 attcctttcc ctcccgacag ccgaaaccca aacacaggtt ttcggctgtt ttcgccccaa
     9661 atacctccta attctaccca aacaccccct taatcctccc cggatacccg ataatcaggc
     9721 atccgggctg ccttttaggc ggca
//
LOCUS       DAXHTY010000071         9602 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXHTY010000071 DAXHTY010000000
VERSION     DAXHTY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.071"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(81..383)
                     /locus_tag="ACFNMK_001517"
                     /pseudo
     CDS             complement(81..383)
                     /locus_tag="ACFNMK_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(597..983)
                     /locus_tag="ACFNMK_001518"
                     /pseudo
     CDS             complement(597..983)
                     /locus_tag="ACFNMK_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(985..1416)
                     /locus_tag="ACFNMK_001519"
     CDS             complement(985..1416)
                     /locus_tag="ACFNMK_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6364760.1"
                     /translation="MDTKHLPSTCRDAASAVCTKTPEYYLNHGKWPDGNSNAGVASSS
                     TIKGKYVEKVEVAKGVITATMLSTGVNNEIQGKKLSLWAKRQNGSVKWFCGQPVKRDD
                     ANAKDDVTAATGTDKIDTKLKKPFYPISFRTDRPGFPPARE"
     gene            complement(1396..1734)
                     /locus_tag="ACFNMK_001520"
                     /pseudo
     CDS             complement(1396..1734)
                     /locus_tag="ACFNMK_001520"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1973..2323)
                     /locus_tag="ACFNMK_001521"
                     /pseudo
     CDS             complement(1973..2323)
                     /locus_tag="ACFNMK_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(2538..2744)
                     /locus_tag="ACFNMK_001522"
                     /pseudo
     CDS             complement(2538..2744)
                     /locus_tag="ACFNMK_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(2773..3086)
                     /locus_tag="ACFNMK_001523"
                     /pseudo
     CDS             complement(2773..3086)
                     /locus_tag="ACFNMK_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            complement(3164..3250)
                     /locus_tag="ACFNMK_001524"
                     /pseudo
     CDS             complement(3164..3250)
                     /locus_tag="ACFNMK_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
     gene            complement(3269..3487)
                     /locus_tag="ACFNMK_001525"
     CDS             complement(3269..3487)
                     /locus_tag="ACFNMK_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HFC6364761.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YSGLNLNQYGVASPCRTICTVCGFVALS"
     gene            3608..4561
                     /locus_tag="ACFNMK_001526"
     CDS             3608..4561
                     /locus_tag="ACFNMK_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6364762.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVHD
                     AHVVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAEAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHASAVREGYVSFEDAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(4636..6693)
                     /gene="metG"
                     /locus_tag="ACFNMK_001527"
     CDS             complement(4636..6693)
                     /gene="metG"
                     /locus_tag="ACFNMK_001527"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC6364763.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGNECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGCLKDVADSALLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPKVAEN
                     AAKFLNLEAITWANTRETLGKHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(6809..8647)
                     /gene="glmS"
                     /locus_tag="ACFNMK_001528"
     CDS             complement(6809..8647)
                     /gene="glmS"
                     /locus_tag="ACFNMK_001528"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC6364764.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPTDVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLSYHVALARGTDVDKPRNLAKSVTVE"
     gene            8929..9447
                     /locus_tag="ACFNMK_001529"
     CDS             8929..9447
                     /locus_tag="ACFNMK_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364765.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
BASE COUNT         1959 a         2247 c         2843 g         2553 t
ORIGIN      
        1 gaaaccgctc cgccgtcatt cccgcgcagg cgggaatcca gccccgtcgg tgcagaaact
       61 tatcgggtaa aaaggtttct ctagtttggt gtcgattttc ttgtcggtgc tgttgacggc
      121 aggtgcttgg tgtcgatttc gttgttgccg gtggctttgt tgacgtcgtc gttggtggtg
      181 gtggtgtcgt tgcgctgaac cggctgtccg cagaaccatt ttaccgaacc gttttgacgc
      241 ttggcccaca gggagagttt tttgccttgg atttctttgt ttacgccggt tgaaagcatt
      301 gtggcggtaa tgacgccttt tgcgacttca actttctcaa catatttgcc tttgatgtcg
      361 gaggaggatg ccacgccggc ggcatcatta aatcccgtca ttcccacgaa agtgggaatc
      421 taggattcag gactggagaa acctttttac ccgataagtt tctgcgccga caaacctaga
      481 ttcccgcctg cgcgggaatg acgaagtttt ttagtcattt gccgtgattc gggcaatact
      541 cggtaacggc tgattttttg gaagtatttg aaagcgaaac cgggtgtttt tgtacggctg
      601 gcagatgaag cgtcgcggca ggttgacggc aggtgcttgg tgtcgatttt gttaccgctg
      661 tcggcggtga cttcgtcggt gccggctttg gctttggcgt cggcgcgctg aaccggctgt
      721 ccgcagaacc attttaccga accgtcttga cgcttggccc acagggagag ttttttgcct
      781 tggatttctt tgtttacgcc gcttgatttc atttcggcgg taacgacgcc gtttttgact
      841 tcaacgcttt taacatattt gcctttgatg tcggtggcgg aggatgccac gccggcagaa
      901 gtgttgtttt tcggccattc gccgtgattc aggtaatact cggtgacggc tgatttttga
      961 ccttcagcca aaaggatagc ttcgtcattc ccgcgcaggc gggaatccag gtctgtcggt
     1021 acggaaactt atcgggtaaa aaggtttctt tagtttggtg tcgattttgt cggtgccggt
     1081 ggcggcggta acgtcgtctt tggcgttggc gtcgtcgcgc ttaaccggct gtccgcagaa
     1141 ccattttacc gaaccgtttt gacgcttggc ccacagggag agttttttgc cttggatttc
     1201 gttgtttacg ccggttgaaa gcattgtggc ggtaatgacg ccttttgcga cttcaacttt
     1261 ctcaacatat ttgcctttga ttgttgaaga agatgccacg ccggcattac tgttgccgtc
     1321 aggccatttg ccgtgattca ggtaatactc gggtgttttt gtgcaaacgg cagatgctgc
     1381 gtcgcggcag gttgacggca ggtgcttggt gtcgatcttc ttggtggcgt cgttgtcggc
     1441 ggcaacgtcg tctttggcgg tgtcgttgcg cgtaaccggc tgtccgcaga accattttac
     1501 cgaaccgtct tgacgcttgg cccacaggga gagttttttg ccttggattt ctttgtttac
     1561 gccggttgaa agcattgtgg cggtaatgac gccgtttgcg actgtaactt ccttaacata
     1621 tttgcctttg atgtcggagg gggtggatgc cacgccggca gaagtattgt tttttggcca
     1681 tatgccgtga ttcaggtaat actcggtgac ggctgatttt tgaccttcag ccaaaagaat
     1741 gacaaatttt aggtttctta ttttggtttt ctgtctttga gggaatgatg aaattttgag
     1801 ttttaggaat ttatcgggag caacagaaac cgctccgccg tcattcccgc gcaggcggga
     1861 atctaggtct gtcggtacgg aaacttatcg ggaaagcggt ttcttgagat tttgcgttct
     1921 ggattcccac tttcgtggga atgacgggat gtaggttcgt gggaatgacg gtttaggtat
     1981 ttttatagaa agccgtaggt ggtgtttcta tgcaaccggc agttgattca ttgcggcagg
     2041 ttgacggcag gtgcttggtg tcgatttttt cgttgccgct gtcggcggta acgccgtcgg
     2101 cgttgttggc ggctttggcg tcgcgctgaa ccggctgtcc gcagaaccat tttaccgaac
     2161 cgtcttgacg cttggcccac agggagagtt ttttgccttg gatttctttg tttacgccgc
     2221 ttgaagccat ttgggcggta atgacgccgt ttgcgactgt aacgctttga acatatttgc
     2281 ctttgatgtc ggtggagacg gatgccacgc cggcggcatc attaaatccc gtcattccca
     2341 cgaaagtggg aatctagaac gcaaaatctc aagaaaccgt tttacccgat aagtttccgt
     2401 gccgacagac ctagattccc gcctgcgcgg gaatgacgaa gtttttcggc catgtgccgt
     2461 gattcgggca atactcggta acggctgatt ttttggaagt gtttgaagtc ggcgcgtggt
     2521 gtttctatgc aaccggtaga tgaatcatcg cggcaggttg acggcaggtg cttggtgtcg
     2581 atcttcttgg cggcgtcggt gtcggcggcg acggtgtcgt ctttggcggt gtcgttgcgc
     2641 gtaaccggct gtccgcagaa ccattttacc gaaccgtctt gacgcttggc ccacagggag
     2701 agttttttgc cttggatttc tttgtttacg ttgcttgaag ccattatgtc agacggtatt
     2761 gcccgggcag ctttattcgt acactttcag cagctcgact tcaaatatca aagtggcgtg
     2821 cgggggaatc acgccgccgg cgccgtgtgc gccgtagccc atttccgaag ggatggtcag
     2881 cttgcgtttg ccgccctcct tcattccgcc gaagccttcg tcccagcctt tgatgacttg
     2941 tccgacgccg agcgtgatgg tcagcggctg gcggcgaatt tggtgccgtc ttccagccaa
     3001 cctgtgtaat gcacggtaat ctctttgcct ttaactgctt cttttccgaa gccttcctgc
     3061 aaatcttcaa taatcaggct gcccatattt gtcctttcgt tgcttgttgg ttaaaacggc
     3121 aagggtaaca taccgtccgg cgaagtcaaa tgccgctcaa acgtcagctg ctccggtgca
     3181 gctgaaacgg ctgtgtttgt ttgactgttt tatttttttc gtaaaggttc catgcttttt
     3241 catggaaata tagtggatta aattttaatc aggacaaggc gacgaagccg cagacagtac
     3301 aaatagtacg gcaaggcgag gcaacgccgt actggtttaa atttaatcca ctatagaaaa
     3361 cgacggtgtt gattaggggc tcgaccagcg cgaccgcccc cgatacgcct atactgcccg
     3421 tcagtacata ggttacactg aaggcggtgc tgaaatgcag tgcggcaaaa gtcaaggttt
     3481 taagcatcat catctcccta attgaaattt acggatagat gataaagatt atcataaggc
     3541 tgcgcggttt aaatttgcta tttgttgtta gtgtaaataa atcatttttt aaatggggat
     3601 agaaattatg aatcataaaa agatcgttgt tttggatgcg gatactttgc ccggccgggt
     3661 ttttcatttt gattttccgc acgagctcgc agtttacggt acgacaggtg cggatgaaac
     3721 ggcagaacgg gtgcacgacg cacatgttgt cattaccaat aaggtaatga tttccgccga
     3781 cattattgcg gctaatccgc agttggagct gattgccgtc agtgcgaccg gtgtgaacaa
     3841 tgtcgatatt ggggcggcgg aggcggcggg cgttgcggta tgcaatgtcc gcgcctacgg
     3901 aaacgaatcg gttgcggaac acgcctttat gctgatgatt gcgttaatgc ggaatctgcc
     3961 tgcctatcag cgtgatgttg cggccggttt gtgggaaaag tcgccgtttt tctgccatta
     4021 cggcgcgccg attcgggatt tgaacggcaa aacgctggcg gttttcggac gcggcaatat
     4081 cggacggacg cttgccggat acgcgcaggc attcggtatg ggggtggtgt ttgccgaaca
     4141 caaacacgcg tccgctgtgc gcgaaggcta tgtttccttt gaagatgcgg tacgggctgc
     4201 tgatgtgttg tcgctgcact gtccgctaaa cgcccaaact gaaaatatga taggcgaaaa
     4261 cgaattgcgg cagatgaagc ctggcgcggt tttaatcaat tgtgggcgcg gcgggctggt
     4321 ggatgaaaac gcgctgcttg ccgcactcaa atacgggcag atcggcgggg ctggtgtcga
     4381 tgttttgacg gaggagccgc caaggggcgg caatcccctg ctgaatgcac gacttcccaa
     4441 tctgattgtt acgccgcata ccgcgtgggc gagccgcgag gctttggaca ggctgttcga
     4501 tatattgttg gcgaacattc acgcctttgt gaaaggcgag gcgcaaaacc gggtagtttg
     4561 agcttgtcgg gataacgaaa aacaatgccg tctgaacgcc caaagggttc agacggcatt
     4621 gtttgagatt tctacttaac cgactttgtc gcccggttgc gcgcctgcat ccacatccaa
     4681 gagcttcagt ttcccgtctg ccgtggcagc ggagagaatc atgccttcgg atacgccgaa
     4741 ttttgccatt ttgcgcgggg cgaagttggc gacggcgatg accatgcggc cgttcaattc
     4801 ggcaggattt gggtaagacg cggcgatgcc ggagaagatg atgcgttttt caaaaccgaa
     4861 atcgaggtcg aattttaaga gtttggtgct gccttcgacg gcttcgcagt tcaatacttt
     4921 ggcgacgcgc atatcgattt tcataaagtc gtcgaagctc gcctgttcgg cgactttttc
     4981 gtatttgctc tcttcggcgg caggcgctgc tgctgctgcg atactttgtt tgttggcttc
     5041 gattaaatcg tccacttgtt tttgctccac tcgttgcatt aaatgttcgt atttgttgat
     5101 ggcgtgtttg cccaaggttt cgcgtgtatt tgcccaagtg atggcttcca aattcaggaa
     5161 tttggcggca ttttcggcca ctttcggcaa cacgggggcg aggtaggcgg tcagcatggt
     5221 aaaggcgttg atgagttcgc tgcatacttc gtgcaggcgt tcgtcttggc cttcttgttt
     5281 ggcgagttcc caaggtttgt tggcatcaac gtattcgttg acaatgtctg ccaaggccat
     5341 gatgtcgcgc agggctttgg cgtattcgcg gctttcgtag cattcggcaa tggcttcgct
     5401 ttgtgcggtc agttttgcca gcaatgcgct gtcggcaacg tctttcagac agccttcaaa
     5461 gcgtttggcg atgaaacctg aggcgcgggc ggcgatgttg acgtatttgc cgacgaggtc
     5521 gctgttgacg cggctgataa agtcttgcag gttcaaatcg atgtcttcga ttttgctgtt
     5581 gagtttggcg gcgatgtagt agcgcatcca ctcggggttt aaaccttgtt ccagatagga
     5641 tttggcggtg ataaacgtgc cgcgcgattt ggacattttt tgtccgtcta cggtcaaaaa
     5701 gccgtgtgcg tacacgccgg tcggggcgcg gtggccggag aaatgcagca tggcaggcca
     5761 gaacagggcg tggaaataga ggatgtcttt gccgatgaag tggtacatct cggtttggct
     5821 gtcggctttg aagtattcgt caaaatcgac gccgatgcgg tcgcacaggt ttttaaacga
     5881 cgccatgtag ccgacgggcg cgtccagcca gacgtagaag tatttgcccg gcgcgtcggg
     5941 gatttcaaaa ccgaaatacg gcgcgtcgcg ggaaatatcc cagtcggaca gggtggtttc
     6001 ttcaccttcg cccagccatt ctttcatttt gttgagggct tcggcttgca gatggggctt
     6061 gccgtcgtgc gggttgttgc cggaagtcca tgctttgagg aagtcggcgc attctcccag
     6121 tttgaagaag aagtgttcgg attcgcgcaa ttcgggtttc gcgccggaaa cggcggaata
     6181 cgggttaatc agttcggtcg gggaataggt cgtgccgcag acttcgcagt tgtcgccgta
     6241 ttggtcttgg gcgtggcatt tcgggcattc gcctttaacg aagcggtcgg gcaggaacat
     6301 ttgtttttcg gggtcgaaaa gctgctcgat gacgcggctc tcaatcttgc cgttggcttt
     6361 cagcgcgcgg taaatgtctt gggaaaactg tttgttttca ggggaatggg tgctgtaata
     6421 attgtcgtaa ccgatgaaaa agccagtaaa gtcggcgagg tgctcttcgc gcactttggc
     6481 aatcatgtct tcgggcgcga tgccttgttt ttgcgcggca agcatcacgg gcgtgccgtg
     6541 ggtgtcgtcg gcgcagcagt agtggcattc gttgccgcgc agtttttgaa agcgcaccca
     6601 aacgtcggtt tggatgtgtt cgaccatgtg gccgaggtgg atgctgccgt tggcataggg
     6661 cagggcggag gtaacgagga ttttgcgtgt catattgtgc tttgcaaaca atgggtaaag
     6721 gcggattata ccgcaaatca aacggggaaa tgccgtctga agcctgaaaa atcgggcttc
     6781 agacggcatg ttcgcaaact atccggactt attcgaccgt aacggatttc gccaggttgc
     6841 gcggtttgtc cacatcagta ccgcgtgcga gggcgacatg gtaggaaagg agctgcacgg
     6901 ggatagtatg cacgacgggg gataattctc cgacgtggcg cggtgcgcgg ataacgtgca
     6961 caccttcggt ggcattaaaa ttgctgtcga ggtcggcaaa gacgaaaagt tcgccgccgc
     7021 gcgcgccgac ttcctgcata ttggctttga ctttgtccaa caggctgtcg ttgggtgcga
     7081 tgacgacgac gggcatgttt tcgtccacca aggcaagcgg cccgtgcttc agttcgccgg
     7141 caggataggc ttcggcgtgg atgtaggtga tttccttcag ttttaacgcg ccttcgaggg
     7201 caatcgggta atggatgccg cgccccaaaa acagcgcgct ggttttcttg gcaaactgtt
     7261 gcgcccaagc ggcaatttgc ggttcgaggt ttaaagcgtg ctgcacgctg ccgggaagct
     7321 ggcggagttc ttcggtataa cgtgcttcat cttcgccgga taccaagccg cgtaccttcg
     7381 ccagcgttac cgccaaaccg aacagtgcga ccagctgcgt ggtaaatgct ttggtcgagg
     7441 cgacgccgat ttccgcaccg gcacgggtat aaagcacgag gctgctttcg cgcggcaggg
     7501 cggattccat cacgttgcaa atggagaggc tgtggcggtg tcccaaagat ttggcgtatt
     7561 tcaaagcctc catcgtgtcc agcgtttcgc cggattggga aatggtaatg accagttggt
     7621 ccggatcggc aatcacgctg cggtatcggt attcgctggc gatttcgacg tcggtcggaa
     7681 ttttggcgat ggattcgagc cagtatttgg cggtcagtgc ggcgtaatag gacgtgccgc
     7741 aggcaaggat tttgacgctg cggatgcttt caaatacgct tttggcgttt ttgccgaagt
     7801 tttcggggat gaagccgccg tcgaggaaaa cctccgccgt gtctgcaatc gcgcggggct
     7861 gctcgtggat ttccttttgc ataaagtggc tgtacggccc cagctccaaa gaggcgagcg
     7921 agagttcgga taccttgact ttgcgttcgg caggcaggcc gtttttatcg gtcagccttt
     7981 tgatgccgtc tgaagccagc agtgcgatgt cgccgtcttc aaggtacgcc acgcggcgcg
     8041 taaaggcgat gacggcggac acgtccgaag cgataaaggt ttcatcgtcg cccaaagcga
     8101 ccaaaagcgg gcagcccata cgcgccacaa ctaattcatc aggcttgtct tgggcaataa
     8161 ccgcgatggc gtatgcgccg tggaaacgtt tgaccgcttc ctgcacggct tcaaacagcc
     8221 tgccgccgtt ttgcgcgtat tcgtgattga tgctgtgggc gatgacttcg gtatccgttt
     8281 gcgattcaaa ccggtatccc aaaccttcca agcgtttgcg ttcgctttca aagttttcga
     8341 tgatgccgtt gtggacgacg gctatcatcc caccggaaat atgcggatgg gcattcggct
     8401 cggtaacgcc gccgtgtgtc gcccaacgcg tatgcccgat gccgatgccg ccgctgatgc
     8461 ctttttcgcg tgctgcgtct tccataagct gcacgcgtcc gacgcggcgc acgcgtttga
     8521 ttttgccgtc ggtgttgacg gcaatgcctg atgagtcata accccggtat tcgaggcgtt
     8581 tgaggccgtc ggtcagaaaa tcgacgacgt tgtgatgggc gcggatggcg ccgacgatac
     8641 cgcacataac tgttccttag tatccggttg aaaaaaaaca ggcgcggacg gctcccgtgc
     8701 cgcaccttcc tcttcggatt ataaaccgcc tcccgcgccg aaaaacagca aaatgccgtc
     8761 tgaaggcttg ggcttgctca aaaaagggag ggatttcccc gcctgaccgg gatgggcgtt
     8821 cagacggcat tgcctgccgc cggtttatag tttttacaaa tcaatattga caagctgaaa
     8881 aaaaaaaaca atatactcgc tcggtcttaa tgttaacgga gtatggaaat gaaacaaatg
     8941 cttttagccg tcggcgtggt ggcggtgttg gcgggctgcg gcaaggatgc cggcggttac
     9001 gagggttatt ggcgcgaaaa atcggacaaa aaagagggta tgattgccgt caaaaaagaa
     9061 aaaggcaatt acttccttaa taaaatcaac gtgtttacag gtaaggaaga atctatgctt
     9121 ttgtctgaaa aagacggcgc gctttcgata aacacgggga taggggaaat cccgatcaaa
     9181 ctttccgatg acgggaaaga gctgtatgtc gaacgcaggc agtatgtcaa aaccgatgcg
     9241 gcgatgaagg acaaaatcat cgcccatcag aaaaagtgcg gacaaacggc acaggcatat
     9301 ctcgacgcgc gaaatgcgtt gccgtcaaac cagacgtatc agcagcatca ggcggcgatc
     9361 gagcagttga aacggcggtt tgaagccgag tttgacgaat tggaaaaaga aatcaaatgc
     9421 aacggcaaac cgacattgtt gttttagcgt aggacaaccg ggaggatgcc gccgtccgaa
     9481 tcggacgtgc ggtttctgta ccggtacggg cgggcaggaa tgtccgcttt ttttgttcgg
     9541 acgcgtttga atacccgttt gattccgtcc gtttgcaaag ggtatttttg ttcgggcgga
     9601 aa
//
LOCUS       DAXHTY010000072         9447 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXHTY010000072 DAXHTY010000000
VERSION     DAXHTY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9447
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.072"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..247)
                     /locus_tag="ACFNMK_001530"
     CDS             complement(<1..247)
                     /locus_tag="ACFNMK_001530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6364766.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYID"
     gene            486..1385
                     /gene="crgA"
                     /locus_tag="ACFNMK_001531"
     CDS             486..1385
                     /gene="crgA"
                     /locus_tag="ACFNMK_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC6364767.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPSSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1609..4824)
                     /gene="carB"
                     /locus_tag="ACFNMK_001532"
     CDS             complement(1609..4824)
                     /gene="carB"
                     /locus_tag="ACFNMK_001532"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC6364768.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECEARPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSNDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4831..5379)
                     /locus_tag="ACFNMK_001533"
     CDS             complement(4831..5379)
                     /locus_tag="ACFNMK_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364769.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(5382..5945)
                     /locus_tag="ACFNMK_001534"
     CDS             complement(5382..5945)
                     /locus_tag="ACFNMK_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC6364770.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            complement(6796..7185)
                     /locus_tag="ACFNMK_001535"
     CDS             complement(6796..7185)
                     /locus_tag="ACFNMK_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC6364771.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7477..7602)
                     /locus_tag="ACFNMK_001536"
                     /pseudo
     CDS             complement(7477..7602)
                     /locus_tag="ACFNMK_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7656..8789)
                     /gene="carA"
                     /locus_tag="ACFNMK_001537"
     CDS             complement(7656..8789)
                     /gene="carA"
                     /locus_tag="ACFNMK_001537"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC6364772.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYASGLIIRDLPLLHSNFRASESLHDYL
                     VRNQTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2133 a         2406 c         2422 g         2486 t
ORIGIN      
        1 cgtctatgta ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa
       61 tcacagcatc catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa
      121 tcacggtttc ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa
      181 gcgtgcggtt taacccgccg tgagaatgtc cgaacgcctt gccgccgtct aataataaaa
      241 tattcatttg ttacctcgtt tgtgaattga tgaatgtatt gttccgtttt actttataat
      301 ttaaaagtgc aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgtttcaac
      361 acacgggatg acacataaag cgtctcccta tgtgccgtcc tgattcggaa ggggttacac
      421 ccctcccaaa taaagtctga tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa
      481 acacgatgaa aaccaattca gaagaactga ccgtatttgt tcaagtggtg gaaagcggca
      541 gcttcagccg tgcggcggag cagttggcga tggcaaattc tgccgtaagc cgcatcgtca
      601 aacggctgga ggaaaagttg ggtgtgaacc tgctcaaccg caccacgcgg caactcagtc
      661 tgacggaaga aggcgcgcaa tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag
      721 cggcggaaac cgaaatgctg gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt
      781 ccgcgatgcc gatggtgctg catctgctgg cgccgctggc aacaaaattc aacgaacgct
      841 atccgcatat ccgactttcg ctcgtttctt ccgaaggcta tatcaatctg attgaacgca
      901 aagtcgatat tgccttacgg gccggagaat tggacgattc cgggctgcgt gcacgccatc
      961 tgtttgacag ccgcttccgc gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc
     1021 cgcaatctac agaagagctt gccggccacc aatgtttagg cttcaccgaa cccagttctc
     1081 taaatacatg ggcggtttta gatgcgcagg gaaatcccta taagatttca ccgcacttta
     1141 ccgccagcag cggtgaaatc ttacgctcgt tgtgcctttc aggttgcggt attgcttgct
     1201 tatcagattt tttggttgac aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg
     1261 aacaaacctc cgataaaaca caccccttta atgctgttta ttacagcgat aaagccgtca
     1321 atctccgctt acgcgtattt ttggattttt tagtggagga actgggaaac aatctctgtg
     1381 gataattcta taaaaacctg tcataaaatt gtttttactc acttaataac tttgagaaaa
     1441 aataaataag atgatgtata gccgcggcaa tggcagtcta tatggggagc gtgatagggc
     1501 tgttccttat gtacggcttg tgtgtggcaa catcggcttt ccaaatgaca aaatgccgtc
     1561 tgaaaccgca aaacggtttc agacggcatt ttcaacctga ttcaggtatc agttgcggtt
     1621 tttcaaacgc ccgtgcaact cttgaacgct gtacacgccc agatggtcgc ggcttttcgc
     1681 gccttcgctc atcgcttcgc cgccggcagt ggtggtgtat tgcggcacac gttgctgcaa
     1741 tgcgctttgg cggatgatgt ggctgtcgga cacggattgc ggatcgttgg aaacggtatt
     1801 cacgaccagt gcgatttcac cgtttttcag cgcgtcgccg atgtgcgggc ggccttcggg
     1861 gactttattg atggtttgga caatcagccc gtgttcggtc aggtattgcg ccgtgccgcg
     1921 tgtggcgcag atgccgtagc ctaaggcttg gaagtttttg gcggttttaa tgacgcgttc
     1981 tttgtcttct tcgcgcacgg agaggaagat tttgccggtc gggttgaggc gttcgcccgc
     2041 gccgagttgg gctttgtagt aggcttcgcc gaagcttgcg cccacgccca tgacttcgcc
     2101 ggtggagcgc atttccggac ccaaaatcgt atccacgccc gggaatttga tgaaggggaa
     2161 cacggcttct ttaacggcat agaaatcggg gacaacttct ttttccacgc cttgttcttt
     2221 cagggagatg cctgccatgc agcgcgcgcc gactttggcg agcggcacgc cggtggcttt
     2281 ggagacgaag ggcacggtgc ggctggcgcg cgggttcact tccaatacga acactacgcc
     2341 gtcctgcacg gcaaactgca cgttcatcag tccgaccacg cccagcgcgt acgccatcgc
     2401 tttggtttgg cggcggattt cgtcttggat ttcttcgctt aaggagtagg gcggcagcga
     2461 gcagccggag tcgccggagt ggatgcccgc ctgttcgacg tgctgcatga tgccgccgat
     2521 aaccacatct ttgccgtctg aaacgcagtc cacatcgact tcaatcgcgt tgttcaggaa
     2581 gaagtcgagc aacacggggc tgtcttcgga aacctgcacg gcttcgcgca tatatttttg
     2641 caactgctcg gcggagtgga caatctgcat cgcgcgtccg cccaaaacat aagacgggcg
     2701 cacgaccagc ggataaccga tttcttcggc tttgacgagt gcttcttctt cgttgtgggc
     2761 gatgcggttg ggcggctggc gcaggcctaa gtcgttcaac actttttgga agcgttcgcg
     2821 gtcttcggcg gcatcgatgc tgtcggcgga cgtgccgatg atgttcacgc cgttttcaac
     2881 cagcgcgttg gcgagtttga gcggggtttg accgccgtaa tgcacaatca cgccccacgg
     2941 gttttcggtg cggacgattt ccaacacgtc ttccaatgtc agcggctcga aatagaggcg
     3001 gtcgctggtg tcgaagtcgg tggacacggt ttctgggttg cagttgacca taatcgtttc
     3061 aaaaccggat tcgcgcaggg cgagcgcggc gtgaacgcag cagtagtcaa actcgatgcc
     3121 ctgaccgatg cggttcgggc cgccgccgag aatcatcact tttttgcggt cggaaggacg
     3181 cgcttcgcat tcctcttcgt aagtggaata gaggtaggcg gtttcggtgg cgaactcggc
     3241 ggcgcaggta tcgacgcgtt tgtaaaccgg atgcagcttc agcgcgtagc ggtgttcgcg
     3301 aacttctttt tcgcttacgt tcaacaattg tgccaaacgt ttgtcggaga agcctttgcg
     3361 tttcagacgg cgtagggcgg cgaaatccaa atcactcaaa atgccgtctg aaaccgcttt
     3421 ttcttccttc atcaagtctt cgatttgcgc caggaaccaa gggtcgatgg cgcagatttc
     3481 gtggatttct tccagcgtga agcccgcgcg gaacgcgtct gccacaaaca gcatacgttc
     3541 ggggccgggg tttgccagtt cgcggcggat ttccgctttg tcggagcttc tcggattgaa
     3601 gccgcacaag cctgtttcca agccgcgcag ggctttttgg aagctttcct gaatcgtgcg
     3661 gcccatcgcc atcacttcgc ccacggattt catctgcgtg gtcaggcggt cgtctgcggc
     3721 agggaatttt tcaaacgcaa aacgcgggat tttggttacc acatagtcga tggacggctc
     3781 gaatgacgcg ggggttttgc cgccggtgat gtcgttgcgc aactcgtcca gcgtaaagcc
     3841 gaccgccagt ttcgccgcca ccttcgcaat cgggaagccc gtcgctttgg aagccagcgc
     3901 ggacgaacgg ctcacgcgcg ggttcatctc aatcacaatc atctcgccgt tttcagggtt
     3961 tactgcaaac tgaacgtttg agccgccggt atccacgccg atttcacgca acactgccaa
     4021 cgaagcgttg cgcatgattt ggtattcctt gtccgtcagc gtttgcgccg gcgcaaccgt
     4081 aatcgagtcg cctgtatgca cgcccatcgg gtcgaagttt tcaatcgagc agatgatgat
     4141 gcagttgtcg ttcttatcgc gcaccacttc catctcgtac tctttccagc cgagaacgga
     4201 ctgctcaatc aacagctcgt gcgtgggcga cgcatcgaaa ccgcgttcgc aaatcgccaa
     4261 aaactcgtct ttattgtagg caatgccgcc gcccgaaccg cccatggtga aagaaggacg
     4321 aatcagggta gggaagccga cctgttcttg cgccgccaaa gcttcgttca tcgtgtggca
     4381 gacaaaagat ttcgggcaag acaaaccgat tttttccatc gcttctttaa agcggccgcg
     4441 gtcttccgcc ttgtcaatcg cgtcttccgt cgcgccaatc agctcgacat tgtattttgc
     4501 cagcacgccg ttgcgtgcca aatccagcgc acagttcagc gcggtctgac cgcccatcgt
     4561 gggcagaatc gcatcaggcc gctccttggc gataatcttc tccaccgtct gccacataat
     4621 cggctcgatg taggtaacat ccgccatttc agggtcggtc ataatcgtgg cggggttgga
     4681 attcaccaaa atgactttat agccttcttc acgcaaagcc ttgcaggcct gtgcgcccga
     4741 atagtcaaat tcgcaggcct gaccgataac gatagggccg gcgccgatga taaggatgga
     4801 ttttaggtcg gtacgtttgg gcatgggtgg ttactcttga attaaagaag gttgttttgt
     4861 tacatagctt ttaagaaaag tatttccctt ttctaaaatt gaagctaaaa catcatcttc
     4921 tatgttagat aaaatattct ctaaatctgc gcttgattta attttaaaga attcttttgt
     4981 ttgagaatgg tatacaagag aaggtttatt tagctgtaaa agttctgcta tattatttaa
     5041 tgaaccaata ctcttaccat cccataaaat aaagccataa tcagcaattt ctgccatttt
     5101 cttatctttt gctgtataaa atacccttcc agttcctttg ctgtctactt ggacaaactg
     5161 ccaattcccc acattatttc ggtaaatttt gccagaaaag taaatatgca cattagcata
     5221 atcttgttct tgtagaaatt tttggatagc tttatcagcc ccattagcat ctccaataac
     5281 aatatcaaaa ttattgctaa gaatattgtt aattctttct cgtatttgtg gatttagtcg
     5341 tgaaatgctg cgtgaaccag aaaagaaaat agtgcgcatt tttatcctct agtctttgtc
     5401 attgctaaaa caattacaga tctagcattt tttgaaaaca atttttcagt tgatatttcc
     5461 aatgttgctc cgctatcaaa taaatcatca attactaaaa tattcttatt ggatagatct
     5521 atattgtcaa ttgtgatgga attattcagt atttctagtt tttctgattt ttcttcaata
     5581 tttttgagtg gtgtgtgtga gctagatttt cttaaaatag gagaatatgg aatattcaag
     5641 cgatcactta gttctttggc aattaattga actggttgat taatacgctc tgttgtaaaa
     5701 ggggcgggaa caattaaatt tatagattct aaaccactaa atttttgtaa aatatagtct
     5761 accagcaaag aaacattttg ggtttgatta cgatatttta attgataaac ccattctcca
     5821 atcacacttc tcttgctatc aaagtgagga tgccctaatt catcataacc taacaaaata
     5881 cttttttgca tatgatggtc taaagcaaat ccttttgtcc agttaccatt tagttctatc
     5941 gccatttcat tgactccttg tcaaaattaa gtataacgag caatcctagc aagccgtagc
     6001 ccgcatgtag ggtatgtgcg gtacgcacgc acgcgttctc cattttccct gcaaccccaa
     6061 cccaccgcgt gcgtgccttg cggcacactc tctgccgatg agttcaaagg tcgtctgaaa
     6121 aaacgttcgc ggcgtttgtt ccacctgcct tgcgataaag gctgcctgaa aaattcccgc
     6181 tgttgatatt tcgggcattc tttctcaaac cgcctgcaac cgcaaaatcc gctgcaacct
     6241 gccccctccc ccgtggggga gggttgggga gagggcattc cccgaatttc ggcaaccttt
     6301 cccaacaact caaccaccga aatacaagcc ttgtggcttg ttgccctctc tctagctctc
     6361 tcccacggga gagaggacgg agtggctgta gggctaaggg ttctgcaagg aaacaggttg
     6421 tgcaggttgc tttttgcttt tcagacgacc tgaaatcaac ggtaaatcca agtcgctttc
     6481 aaaccgcctg caaccgcaaa atctgctgaa acctactccc tcccccgtgg gggagggctg
     6541 gggagagggc attctccgaa tttcggcaac ctttcctaac actctaaccg ccaagacaca
     6601 agccttgcgg cttgttgccc tctctctagc tctctcccac gggagagagg atggggtggc
     6661 tgttggggct aaggtttctg taaactaatc aggttatgca ggctgctttt tgtttttcag
     6721 acggccttta taaatgctca attcagtcgg ctttatccta ttattttcaa taatccaatc
     6781 aaaatcagcc atctgctatc gtgcagcttg cttttccaat tcttgcaaca cgagcaggat
     6841 ttctgtcagc acatcgtttg tctgctgtaa aatctcgtga ttccaaaacc gcagtacggt
     6901 aaagcccaag ctgttgagat atgccgtccg cgcgtggtcg tatgcggctt gttctgtgtg
     6961 ctgccctccg tctgcttcga caatcaactt gggcgttacg cacataaaat caacaatata
     7021 gtttcccatc ggctgctggc ggcggaattt atagccgttc agacgacctg cccgcaggtg
     7081 ctgccacaat tttgcttccg ccccgctcat ttcttggcgc atggctttgg cgcgttggcg
     7141 caagatgggg ttgtcggtgg tcaagagttt ctcgtgcggg ttcattttgt tgtcgtgctg
     7201 tttgctgccc tctctctaac tctctcccac ggggagagag gacggtgcgg ctgtagtgat
     7261 taagggtttt gcaaggaaaa caaggaaaac aagttgtaca ggctgttttt tatttttcaa
     7321 gcgacctgaa atcaacgctt gtaaatccaa gtcgcttttc aaactgcctg caaccgcaaa
     7381 atctgctgca acctaccccc tcccccgtgg gggagggctg gggagagggc attttccaag
     7441 ttgcggcaat ctttcccaaa tcccttagtc ttctaaaata caagccttac ggttgttgcc
     7501 aaatgaaact tcttccatac ccaaaatttg cgtgtaaaac gcgatggttc ggtcaatgtc
     7561 ggcaacggtc agtaccagat ggtcgagtgc gctaatcttc atttccggct ttctttttca
     7621 gacggcagca gcatactgtt gccgtctgaa agccattatg cccgtttggc agccttcata
     7681 ttgccaatga atttgtcgaa caaatagccg acatcttgcg ggcccgggct ggcttcgggg
     7741 tggccttgga agcagaatac gggtttgtcg gtcagttcga taccttgcaa ggtgttgtcg
     7801 aacaaggatt tgtgggtaat ccttgcgttg gcgggcaggg tgtcggcatc gacggcgaag
     7861 ccgtggtttt ggctggtgat gacgactttg ccgctgtcca aatcttgcac agggtggttc
     7921 gcaccgtggt ggctgaagcg cattttcagg gttttcgcgc cgatagcgag gctgatgagc
     7981 tggtgtccca agcagatgcc gaaaatcggt ttgccgcttt ccatcagttt ttgcacggct
     8041 tcgatggcgt aggtgcaagg ctcggggtcg ccggggccgt tggacaggaa cacgccgtcg
     8101 ggattgagtg ccaacacgtc ttgtgcgctc gtttgggcgg ggacgacggt caggcggcag
     8161 ccgcgcgagg cgagcatacg caggatgttg gttttcacgc cgaaatcgta ggcgacgacg
     8221 tggtaaggct gttcgtcagg ggtaacgaaa cccttgccca attcccattc gccttccgtc
     8281 cattcgtaag tttccgtgca ggaaacttct tttgccaagt ctttgccgac catactgccg
     8341 aacgcggcga tgagttcttg cgctttttcg attgtggcat ccgcaccggt cagaatcgcg
     8401 ccgccttgcg cgcctttttc gcgcaacagc gtggtcaggc ggcgggtgtc gatgtcggcg
     8461 atggcgacgg tttggttgcg taccaaatag tcgtgcaggc tttcggaggc gcggaagttg
     8521 ctgtgcaaga gcggcaggtc gcggataatc aggccggagg cataaacgct gcggctttct
     8581 tcatcttcgg cgttggtgcc ggtgttgccg atgtgggggt aggtgagggt aacgatttgt
     8641 ttgcagtagg acgggtcggt caggatttcc tgatagccgg tcatcgaagt attgaacacg
     8701 acttcgccgg aagtcgaacc ttcgtaaccg attgatgtgc cgtgaaatac gctgccgtca
     8761 gcgaggacga ggagggcggg ggtgctcatg atgggaatcc tgtttttaat aaaattcttg
     8821 acaatgccgt ctgaagggac ttcggcaggg ggcatccggt cggaaaaatc cggtcaaaaa
     8881 aaaacacgcc gcgcaagata gacgcgtaac gtgcttttcg ggcaagtgcc tataccctga
     8941 aagcagcata ttttaagacg aaacataccc gatttcaagc gcaaacgcgg caggcgggct
     9001 gatatgttcg cgcgaaaacg gaaatccggg gcataaaacc gcccgctccg cgcccactat
     9061 gcccgttacg catttcccca cgtccgcccg gcgaaactat gggaataccc gaatcgtcag
     9121 gaatacccga accgtcattc ccgcgcaggc gggaatccag acctgtcggc acagaaactt
     9181 atcgggtaca acggtttctt gagattttac gtcctagatt cccgcctgcg cgggaatgac
     9241 ggtatcggtt tgtcggcgtt tcatttgccc ccctcccgaa aaacgcaaaa aatgccgtct
     9301 gaaagctctg tagggagggc ttcagcccac cgattccatc aatcccacat ttccgccgtt
     9361 ttcaccgaat ccgccccttt tcacaatcca gtcctacgca actgaaccgt cattcccgcg
     9421 caggcgggaa tccggacttg tcggcac
//
LOCUS       DAXHTY010000073         9373 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXHTY010000073 DAXHTY010000000
VERSION     DAXHTY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.073"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(69..458)
                     /locus_tag="ACFNMK_001538"
                     /pseudo
     CDS             complement(69..458)
                     /locus_tag="ACFNMK_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
     gene            571..1008
                     /gene="rnhA"
                     /locus_tag="ACFNMK_001539"
     CDS             571..1008
                     /gene="rnhA"
                     /locus_tag="ACFNMK_001539"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC6364773.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHAGHAENEHADDLANRGAAQFS"
     gene            complement(1081..1494)
                     /locus_tag="ACFNMK_001540"
     CDS             complement(1081..1494)
                     /locus_tag="ACFNMK_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364774.1"
                     /translation="MNKFLATWLAPILVLSGCATIMGESSQTVPIKSQPSGAVFKIQD
                     ETGVIVQSGVTPANVSLPKGNGKYFGRKKYQVSFEKKGYQPLVYKLETNPNGWYIGGN
                     LIFGGLIGYLIVDPLSNGKMFTISPKQVDVPLIAK"
     gene            1646..1999
                     /locus_tag="ACFNMK_001541"
     CDS             1646..1999
                     /locus_tag="ACFNMK_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6364775.1"
                     /translation="MVQLLATEADRLIGEKIRQRRKELGYSAEKLAEHINLSQQQISR
                     YERGVGKINISHLVDIAVFLETPIAWFFQDCLPKPLTVACTPENTPEQQWHSLNPNQK
                     AAFAAFLDTLRNKET"
     gene            1996..2400
                     /locus_tag="ACFNMK_001542"
     CDS             1996..2400
                     /locus_tag="ACFNMK_001542"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC6364776.1"
                     /translation="MNTKPCPICTAQDEDILLQTPNLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
     gene            2440..3624
                     /locus_tag="ACFNMK_001543"
     CDS             2440..3624
                     /locus_tag="ACFNMK_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC6364777.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDAQRVADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQHAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(3687..4220)
                     /locus_tag="ACFNMK_001544"
     CDS             complement(3687..4220)
                     /locus_tag="ACFNMK_001544"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFC6364778.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(4399..6654)
                     /locus_tag="ACFNMK_001545"
                     /pseudo
     CDS             complement(4399..6654)
                     /locus_tag="ACFNMK_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
     gene            7024..8169
                     /gene="nirK"
                     /locus_tag="ACFNMK_001546"
     CDS             7024..8169
                     /gene="nirK"
                     /locus_tag="ACFNMK_001546"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC6364779.1"
                     /translation="MKRQALAAIIASMFALAACGGEPAAQTPAASAEAASSAAQTAAE
                     TPSGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYHYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGSGAAPAASAPAASAPAKSDY"
     gene            8296..9051
                     /locus_tag="ACFNMK_001547"
     CDS             8296..9051
                     /locus_tag="ACFNMK_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC6364780.1"
                     /translation="MKFARLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKASE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQRNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
BASE COUNT         2466 a         2616 c         2185 g         2106 t
ORIGIN      
        1 gaaaacagcc gaaaacctgt gtttgggttt cggctgtcgg gagggaaagg aattttgcaa
       61 aggtctcaac ccatatccaa cgccctgccc gccggtacgg tatcctgagc cgccgcaacc
      121 gcagaatgcg tggcactcat gccgtatttt ttgtgaaaat agtctgccgc cgcgcaatac
      181 agcgacaaac ggatttcggc atcgtccgtt ttcggtttga cagaaaacac ctgctgcggc
      241 gcaaacacgc aatcgccgct ttctgccgac caaacctctg ccgccccgtc cggtgcacgg
      301 acttcgacat cgccctgcaa cacattcagg cagacccatt ccccttcctc agacgaatag
      361 cccgacaaca aaatttccgg caggttttcc actttccata caggcatttg tccgaaacaa
      421 aacaactcgc cgctttgacc cactatccgc tccttcatat tcaaaaataa agttgcacat
      481 tatatgccta tttcaatccg ccgcaatctt tcagacggca cggcgcgcaa accgcttata
      541 atcacgccgg acaccacaca aaggcacaat atggacacac ccgtttacct ttacaccgac
      601 ggcgcgtgca aaggcaatcc cggcgcgggc ggctggggcg tattaatgcg ctacggcagc
      661 catgaaaaag aacttttcgg cggcgaagcg caaaccacca acaaccgcat ggagctgacc
      721 gccgtcatcg aaggactgaa atcgctcaaa cgccgctgca ccgtcatcat ctgcaccgac
      781 tcgcaatacg tcaaaaacgg catggaaaac tggatacacg gttggaagcg caacggctgg
      841 aaaaccgccg ccaaacagcc cgtcaaaaac gacgacttgt ggaaagaact cgacgctctg
      901 gtcggacggc accaagtcag ctggacttgg gtgaaaggac acgcgggaca cgccgaaaac
      961 gaacacgccg acgatttggc aaaccgtggc gcggcgcagt tttcctgact gccgcaccgg
     1021 caagaaaatg ccgtctgaaa ccgctaatgg gctttagacg gcatttttac catacactcc
     1081 ttatttcgca attaacggta catctacctg cttgggtgaa atcgtaaaca tcttaccgtt
     1141 actcaacgga tcaacaatca gataaccaat caggccaccg aaaatcaggt ttccaccaat
     1201 ataccaacca ttcggattgg tttctaactt gtaaaccaac ggctgataac ccttcttctc
     1261 aaaagaaacc tgatattttt tcctgccgaa atatttgccg ttgcctttag gcaacgaaac
     1321 atttgcgggc gttacacctg attgaacaat cacacctgtt tcatcctgaa ttttaaatac
     1381 cgcaccggat ggttgacttt taatcggcac ggtctgactg gactctccca taatcgttgc
     1441 acaacccgac aaaaccaaaa tcggtgcaag ccatgttgcc aagaacttat tcatcaaaat
     1501 catctccaac tgactaaaaa ataaaataca ttaaattaca caataatgat gtaatttgga
     1561 atattttact tatttacaac actgtttaca agagaaaaaa caagataaat atccatagat
     1621 gagtaaatct acaatcggat acaaaatggt acaattgctt gcaacagaag cagaccggct
     1681 aataggtgaa aaaatccgac aacgacgaaa agagttgggc tattctgcag aaaagctggc
     1741 ggaacacatc aatctttctc aacagcagat ttcacgctac gaacgtggcg taggtaaaat
     1801 taatatttcg cacttagtcg atattgccgt ttttctggaa acgccgattg cttggttttt
     1861 ccaagattgc ctgcccaaac cactgacggt agcctgtact cctgagaaca cacccgaaca
     1921 gcaatggcac agccttaatc caaaccaaaa agctgccttt gccgcctttt tggatacttt
     1981 aaggaataaa gaaacatgaa caccaagcct tgccccatct gcacggcaca agatgaagat
     2041 attttgctgc aaacccccaa cctccgcgtc atcgccgtcc ataacgacag cggttcgcct
     2101 gcattctgcc gcgtcatttg gcgtaagcat attgccgaaa tgaccgacct ttcggcagcg
     2161 gaacgcggcg aattgatgga aatggtgtac aaagtcgaag ccgctatgcg ccaagtgttt
     2221 caacctgcca agattaacct cgccagcttg ggcaatgtcg tgccgcacct gcattggcat
     2281 attatcgccc gctttgaaaa cgatgcgtct ttccccgcgc cgatttgggc aaaccccgtc
     2341 cggaaacacg gtatgaccct gccgcaagat tggacggaac agcttaaaaa gctgctttaa
     2401 gcccgccgat gccgtctgaa accgtatgaa aggggaatta tgaccgaacc gacctcccgc
     2461 cgccgtttcc tgaaaacctg caccgccgcc gccggcgcgg gactgcttca ggcttgcggc
     2521 acatccgcca catccgttcc gccccttccc tcttcccatt ccgttgtgaa agctcgaacc
     2581 gtgcctctcc aaacgccacg ccgtcaaagt tcggacggca accttctgcg cgttgtcgct
     2641 tcgtcaggct ttgccgaaga caccaaccgc gtcaacacag ccttaacccg cctttacaat
     2701 gccggtttta ccgtaaccaa ccaacaggcg ggcagccgcc gcttccaacg gtttgctggt
     2761 acggacgcac aacgtgtcgc cgatttccaa gaggtcgctt ccggccgcgt cgccacgcct
     2821 aaagtgctga tgggcttgcg cggcggttac ggtgcggcgc ggattctgcc gcatatcgat
     2881 tttgcttcgc tcggcgcaag gatgcgcgaa cacggcacgc tgtttttcgg attcagcgac
     2941 gtatgcgccg tccagctggc attgttggca aaaggcaata tgatgagttt tgccggcccg
     3001 atggcttata gcgattttgg caaacccgcc cccggtgcgt ttacgatgga tgcctttatc
     3061 aagggtgcaa cccaaaaccg cctgaccgtt gatgttcctt atatccaacg cgccgatgtc
     3121 gaaaccgaag gcatattgtg gggcggcaac ttaagcgtcc tcgcctcgct cgccggcacg
     3181 ccttatatgc ccgacatcga cggcggcatt ttgttcctcg aagatgtcgg cgaacagccc
     3241 taccgcatcg aacgtatgct caatacgctg tatctttcgg gtattttgaa gaaacagcac
     3301 gccatcgtgt tcggcaattt ccgtatggaa aaaattcgag atgtctatga tccgtcttat
     3361 gatttttccg ccgttgccaa ccatgtttcg cgcacggcga aaatccccgt gctgacgggc
     3421 ttcccgttcg gacacattgc cgacaaaatc actttccctc taggcgcgca cgcccgaatc
     3481 cgtatgaacg gaaacagcgg ttattcggtc gcgtttgaag gctacccgac actcgatgcg
     3541 tccgccctga ctttggatac cctgctccca ccgccggatt tgcccatctt ccccgaaagc
     3601 ggtgttgccg atatttcgga ataaacccgc aaacggacaa atgccgtctg aagccttcag
     3661 acggcatttc ccaagacggc ggcagattac agcaatgccc gaatatcggc ttcgatttct
     3721 tcgggcgtaa cactaggcgc aaaacgctcg accacttcgc cgtcgcggtt gacgaggaat
     3781 ttggtaaagt tccatttgat gtcgccttcg tcgcgcttct ctcccaaagc tgcgagcttc
     3841 aacacgaaat ctttaaacag atgattgcct ttatcttgcg gtttgacgga tttcaggtag
     3901 gcatacaagg gcgcggtatt tgctccattg acttcgattt tgtcgaaaat cttaaacttc
     3961 gtgccaaact tcatcataca cacttgggca atttctccgc tgctttcggg agcctgttcg
     4021 cggaactggt tgcacggaaa atccaaaatc tccaagcctt ctgcggtata ttgtgcatac
     4081 agcttctgca aagcctcgta ttgcggggtc agaccgcaac gcgttgccgt gttgacaatc
     4141 agcagaacct tgccgcgata gcctgacaaa tcaaccgcat tgccttccgc atctttcatt
     4201 tgaaaatcgt aaatacccat ttttatcctt atctgatgta aaccgatgcc atctgaaacg
     4261 tgcttcagac ggcatgaaag cagcaattgt atagccgatc aaaataaaaa atccacatcc
     4321 ttttccattc ccgtcccaat ccgcaataaa aaactgcacc cgaaaacggg tgcagttgct
     4381 catttcatac cgcaaaactt atttgtcccg gccgaatacg attttagtag cttggatggc
     4441 aacgcagatt gcaccgccga taaagaccaa gtcagctgcc gtacgtaccc aacgcaaggt
     4501 atcgaggatt tccatttgca ggaactcttc gctgcgggca taccacagac cgtgcgtgat
     4561 ggaggcgtat gcctgaatcg cgccgacagg cagcaggctg ataacaatca taccgaccaa
     4621 gccaccgttg agcagccaga agccccaagt catcagtttg tcatcaaact gcgcgttcgg
     4681 tttcaaataa cgggcaacca acaatacgaa gcccaatgcc aagaaaccgt acacaccaaa
     4741 caaggcggtg tgcgcgtgaa cggcagaagt gttcaaacct tgaatataga acagggaaat
     4801 cggcggattg atcaggaagc cgaatacgcc ggcaccgatc atattccaaa aggcgactgc
     4861 cacgaagcac atcagcggcc aacgcaggcg tttcgcccat ttggacaggt gttggtaaga
     4921 ccaatgctcg tatgcttcac ggcccagcaa caccagcggc acgacttcca aagcggagaa
     4981 gcaggcaccg attgccatag aggcggaggt agagccggag aagtacaggt ggtgcagcgt
     5041 acctggaacg ccgcccaaca taaagatggc ggcagcggcc agagtagaag ctgtggcggt
     5101 actgcggcgg acaaagccca tattgtagaa gacaaaggca aaagcggcag tggcaaatac
     5161 ttcaaagaag ccttccaccc acaggtgaac cacccaccaa cgccagtatt ccattacagc
     5221 aatcggggat ttttcgccat agaacaggcc tggtgcgtag aatacgccca caccgaccat
     5281 agaagcgaca aagattgcca gcaggttttt gtccacgcct ttttctttaa aggcggaaac
     5341 cgtgcaacgc aacatcagga acagccataa cagcagaccg accatcaaaa ggagttgcca
     5401 gaaacgtccc aaatcgaggt attcgtaacc ttggtgtccg aaccagaagt taaattccgg
     5461 gggaaggatg tgcgtcaacg cgaagaagtt gcccgcgtaa gaaccgccga ccacgatgaa
     5521 gagggcgata tagaggaagt ttacgccggc acgttggaac ttgggatctt taccgccgtt
     5581 gacaatcggc gcgaggaaca aacctgccgt caaaaagccg gttgcaatcc agaagatggc
     5641 ggattggatg tgccaagtac gggtcagggc gtaggggaac cagtcggaca tttcaaagcc
     5701 caacgcctcg tcaatgccgt agaaaccctg gccttcgacg gtgtagtgcg cggtcagtcc
     5761 gcccagcaat acttgtacca caaacagggc gaccgtcagg aagacgtatt tgcccaatgc
     5821 tttttgcgaa ggggtcagtt ggattttgga aatcgggtct tcagacggca cttccacttc
     5881 ctcgtgtttg gtcaagaagg aataacccca catcagcaaa ccgatgccca tcagcagaag
     5941 aacaacgctg gtgaatgacc acatatagtt ttcagtggtc ggtacgttgt tgatcaaagg
     6001 ctcgtgcggc cagttgttgg tataagtgaa cgtctcgtca ggacggttgg tcgaagcaga
     6061 ccaagaagtc tagaagaaga agtcgaacag tttttcacgc gcttcttggc ttggcaatgt
     6121 gttgtttttc attgcaaagt gttcgcgggt ggtttgcagc gcgggatcat cgccgtaaac
     6181 accgtggtaa taaggcagga tgctttcgat ggctttcacg cgcgtatcgc tgatgacgac
     6241 gctgccgtct tctttaatac ggctttggtt gcggtattcg tcagccagtc gggttttcag
     6301 aacggcttgt tcttcagggg aaacttcatc gaattttttg ccgtaagtct gttgcgcggt
     6361 caaatccaac caagcggaca actcacgatg cagccagtcc gccgtccagt ccggagcctg
     6421 atatgcgccg tgacccagaa tcgaaccgac ttccataccg ccggtactct gccacgcaga
     6481 ctgacctgcc aaaatatcgt ctttcgtcat cagcactttg cctgatgcgg aaacgacctg
     6541 ttcggggtaa ggcggggctt tcttataaac ctcgctgccc atatagccaa gaatggtaaa
     6601 gcataccgcc agaacggcaa acagcaagta ccacagcttc ttgtactgtc ccattttgag
     6661 agctcctttt aatatagtgg attaaaattc acaaaatatg aatgttaaag attgtagcac
     6721 ggtttaccgc gcaaataaac atttgttcaa agaaactcac atataaaaca aatacatata
     6781 tgataataac tatcattatt ctttagtcgg caactaccct gcctttgcct gatttgccga
     6841 agcccttaag caaatcagcc tatttattgt aatttttagt agctataaag tattagaagt
     6901 atcattttaa gttcatattt tatgaattat ttgacttaaa tcaaaatgtc ggtgatgggg
     6961 caaacgcata atcacaccag ttcttaacca atctccctac ttttcttaca aaaggaaaac
     7021 attatgaaac gccaagcctt agccgcaatt atcgcttcta tgtttgcttt agccgcctgc
     7081 ggtggcgaac ctgccgcgca aacccctgcc gcttccgccg aagccgcaag ctccgccgca
     7141 caaaccgccg ccgaaacacc gtccggcgag ttgcccgtta tcgatgcggt taccacccac
     7201 gctcccgaag tacctcccgc aatcgaccgc gactatcctg ccaaagtccg cgtaaaaatg
     7261 gaaaccgtcg aaaaaaccat gaaaatggac gacggcgtgg aataccatta ctggacattt
     7321 gacggcgatg ttccgggccg catgatccgc gtacgcgaag gcgatacggt tgaagtggaa
     7381 ttttccaaca atccttcttc taccgtaccg cacaacgtcg acttccacgc ggctaccggc
     7441 cagggcggcg gcgcggccgc aacctttacc gctccgggcc gtacttccac attcagcttc
     7501 aaagccctgc aaccgggtct gtacatctac cactgcgccg tcgcaccggt cggtatgcac
     7561 atcgccaacg gtatgtacgg tctgattttg gtcgagccta aagaaggtct gccgaaagtg
     7621 gataaagagt tctacatcgt ccaaggcgac ttctacacca aaggcaaaaa aggcgcgcaa
     7681 ggcctgcaac cgttcgatat ggacaaagcc gttgccgaac agcctgaata cgtcgtattc
     7741 aacggtcatg taggtgctat cgccggcgat aacgcgctga aagccaaagc aggcgaaacc
     7801 gtgcgtatgt acgtcggtaa cggcggtccg aacctggtgt cttccttcca cgtcatcggc
     7861 gaaatcttcg acaaagttta tgttgaaggc ggcaaactga tcaacgaaaa cgtacaaagc
     7921 accatcgttc ctgccggcgg ctctgccatc gtcgaattca aagtcgacat cccgggcagc
     7981 tacactttgg tcgaccactc catcttccgc gcattcaaca aaggcgcgtt gggccaattg
     8041 aaagtagagg gtgcggaaaa ccctgaaatt atgactcaaa aattgagtga taccgcttac
     8101 gccggcagcg gtgcagctcc tgctgcttcc gctcccgcag cttctgctcc tgcaaaaagc
     8161 gattactaaa ttgaacaccc atttttaggc aaacgaacca ctgccgccgt acttcattac
     8221 gcacggcagt ggttttttaa caaccaatct ttcctttcgg aagattgatt ttaactgcct
     8281 gtcaggaggc atgatatgaa gtttgcccgg ttactctttc tctgtgcggc actcgccggc
     8341 actcaagcgg cagctgccga aatggttcaa atcgaaggcg gcagctaccg cccgctttat
     8401 ctgaaaaaag ataccggcct gattaaagtc aaaccattca aactggataa atatcccgtt
     8461 accaatgccg agtttgccga atttgtcaac agccaccccc aatggcaaaa aggcaggatc
     8521 ggttccaaac aggcagaacc cgcttacctg aagcattgga tgaaaaacgg cagccgcagc
     8581 tatgcaccaa aagcaagcga gctgaaacag cctgttacca atgtttcctg gtttgccgcc
     8641 aacgcctatt gcgccgcaca aggcaaacgc ctgccgacca ttgacgaatg ggaatttgcc
     8701 ggacttgctt ccgccacgca gagaaacggc tcaaacgaac caggctacaa ccgcactatt
     8761 ctcgattggt atgccgacgg cggacggaaa ggcctgcacg atgtcggcaa aggccgcccg
     8821 aactactggg gcgtttacga tatgcacggt ctgatttggg aatggacgga agatttcaac
     8881 agcagcctgc tttcttccgg caatgccaac gcccaaatgt tttgcagcgg cgcatctgtc
     8941 gggtcgagcg actcgtccaa ctatgccgcc ttcctccgct acggcatccg taccagcctg
     9001 caatccaaat acgtcctgca caacttaggc ttccgctgcg caagccgata accccttcaa
     9061 ttatagtgaa ttaacaaaaa ccagtacggc gttgcctcgc cttgccgtac tggtttttgt
     9121 taatccgcta tattccgcca tctcttaaga tttacagcga tacacgggtg atttaaggaa
     9181 tgcccgaacc gtcattcccg caacttttcg tcattcccgc aacttttcgt cattcccgca
     9241 acttttcgtc attcccacga acctacatcc cgtcattccc acgaaagtgg gaatctagaa
     9301 tctcggactt tcagataatc tttgaatatt gctgttgttc taaggtctag attcccgcct
     9361 acgcgggaat gac
//
LOCUS       DAXHTY010000074         9256 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXHTY010000074 DAXHTY010000000
VERSION     DAXHTY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.074"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(57..1850)
                     /locus_tag="ACFNMK_001548"
     CDS             complement(57..1850)
                     /locus_tag="ACFNMK_001548"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC6364781.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQTNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            2095..3204
                     /gene="proB"
                     /locus_tag="ACFNMK_001549"
     CDS             2095..3204
                     /gene="proB"
                     /locus_tag="ACFNMK_001549"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC6364782.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTIACQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3217..4479
                     /locus_tag="ACFNMK_001550"
     CDS             3217..4479
                     /locus_tag="ACFNMK_001550"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC6364783.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDEKRIGAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KAGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVW
                     HIETHSTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(4704..7748)
                     /locus_tag="ACFNMK_001551"
     CDS             complement(4704..7748)
                     /locus_tag="ACFNMK_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6364784.1"
                     /translation="MFWIVMIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFHRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESQTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDA
                     WVVEPPEVPKVPMTAIDIQPPPPVSEIYNRTYEPPSGFEQVQRSRIAETDHLADDVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDT
                     EADEGAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLLPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            7913..8593
                     /locus_tag="ACFNMK_001552"
     CDS             7913..8593
                     /locus_tag="ACFNMK_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFC6364785.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     DSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            8622..8981
                     /locus_tag="ACFNMK_001553"
     CDS             8622..8981
                     /locus_tag="ACFNMK_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFC6364786.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASIPPATGNLFANWTGA
                     LLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQLNRPASALSNIFLHTA
                     GSLLLTWLGLKIGAAVK"
     gene            complement(9114..>9256)
                     /gene="mafI"
                     /locus_tag="ACFNMK_001554"
     CDS             complement(9114..>9256)
                     /gene="mafI"
                     /locus_tag="ACFNMK_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein MafI"
                     /protein_id="HFC6364787.1"
                     /translation="NEFDEEDGWPDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVI
                     EE"
BASE COUNT         2019 a         2602 c         2439 g         2196 t
ORIGIN      
        1 ccgcaatgcc gtctgaagcc tttttatgct tcagacggca tcgtctgttt tcacgctcaa
       61 atcgtaccgc tgccttgcgc tttcggtttg gcggcactaa attccagttt gctcaaagcg
      121 gaaaggtagg ctttggcagt ggcaaccaaa acgtcggtat ccgcgccctg accgttgacg
      181 acgcggttgc cgcgtgccag acggacgctg gtttcgcctt ggctttccgt accttgcgtg
      241 acggcgttga cggaataaat ctgcaaagtc gcgccgcttt gcgccacgct ttcaatcgct
      301 ttgaaaattg catcaaccgg accggaaccg gttgcggaag cgcgtttttc ttcgcctttg
      361 atgctgaaca cgatgtcggc gcgcggctct tcgccggttt cggtgctgat tttttgggag
      421 atgaatttgt agctctcggc attcatgctg cccatttcgt cggataccag tgcgtgcagg
      481 tcttcatcga agatttcgcg ttttttgtcg gcgagttctt tgaagcgggc aaacgcggcg
      541 ttcagtgcct cttcgctttc caactcgatg cccaaatccg ccagcttggt tttgaaggcg
      601 ttgcggccgg acaatttgcc caagctcaaa cggtttgttg cccagccgac cgattcggcg
      661 gacataatct cgtaagtttc gcggtgtttc agcaccccat cctgatggat gcccgattca
      721 tgcgaaaagg cattggcacc gacaatggct ttgttgggct gcacgggata gcccgtaatg
      781 gtggacacca gtttggacga aggcacgatt tgcgtggtat cgatgccggt ttccaagccg
      841 aacaagtcgt ggcgcacttt caacgccatc acgatttctt caaccgaagc attgcctgca
      901 cgttcgccca agccgttgac agtacattcc acctgacgcg cgccgccttt taatgcggca
      961 agcgaattgg caaccgccaa gcccaaatcg ttgtggcagt gtgccgacca aacgactttg
     1021 ccgccgttgg gcgttttggc aatcagttcg cggaaaaatt cttcggtttt gtacgggatg
     1081 gaatagccga cggtatcggg aatattgatg gtggtcgcgc ccgcttcaat caccgcgccg
     1141 cagatttcgg caaggaaatc gatttccgaa cgcaacgcgt cttcgcagga aaattccaca
     1201 tcgtcggtgt attcacgagc gattttcacc gctttgaccg ccgcctcaat cacctgcttc
     1261 ggcttcatct tcaatttgta ctccatatgg atggggctgg tggcgatgaa ggtgtggatg
     1321 cgttttttcg gcgcgggcgc aacggcctca cccgcctgac ggatgtcccg ctcgatggcg
     1381 cgggacaatg aacagaccgt tgatttggta atggttttcg caatcgcatt gaccgcctcg
     1441 aaatcgcccg ggctggcagc ggcaaaaccc gcttcgatga tgtccacacc caatttttcc
     1501 agctggcggg cgacgcggat tttttcctct ttggtcatag cggcgccggg cgactgctcg
     1561 ccgtcgcgca gggtggtatc aaaaataatt acgcggttgg tctgtgtcat ttctgttctc
     1621 tccagagatt cgtttttttg taaaaaagca ttcaaatcca gcggtctgcc ttgcgattcg
     1681 gcaagcgcgg tcagcttttg cagttgcgcc agaggcagcg agccgcgcgt gcgccattta
     1741 tagatggcgg cggtcgtcgc ggcattttcg ggatcgtgct gtttcaacgc ttcggcaagc
     1801 gcgttcacgc cgccgaaata agcaaccaag cggtctatat ctaattgcat ggtagtcctt
     1861 gtctaaaata aatcagccta cagcagaaat atggacagga ttatacgcat tttggacaaa
     1921 acggcaatat aaatttgaaa aatctcaata agccgccata gattttatta attttataca
     1981 ttttgtccat tttattgcag acgcggcgtg attgttgaca attgcggcag aacagggcaa
     2041 attcggcggc acaaggtggc aacgagacag gacaaaccaa caaggacgga aacaatgaaa
     2101 tacaaaagaa tcgtatttaa agtcggcaca tcttcgatta cccattcgga cggcagtctc
     2161 tcgcgcggca aaatccaaac catcgcctgc cagcttgccg cattgcatca tgcgggacac
     2221 gagctggtct tggtgtcttc cggcgcggtt gcggcagggt tcggtgcgct gggtttcaaa
     2281 aaacgtccgg tcaaaatcgc cgacaaacag gcttccgccg ccgtcgggca ggggctgctg
     2341 atggaagaat atacggcaaa cctgtcttca gacggcatcg tgtccgcgca aatcctgctc
     2401 agccgcgccg actttgccga caaacgccgc taccaaaatg ccggcggcgc actttccgtg
     2461 ctgctgcaac gccgcgccgt ccccatcatc aatgaaaacg atacggtttc ggttgaggaa
     2521 ttgaaaatcg gcgacaacga cacattgagt gcgcaagtgg cggcgatgat acaggcagac
     2581 ctcttggtgc tgctgaccga catagacggt ctttacacgg gcaacccgaa cagcaatccc
     2641 gatgccgtac ggctggacaa aatcgaacac atcaaccatg aaatcatcga aatggcgggc
     2701 ggatcgggtt cggcaaacgg cacgggcggt atgctgacca aaatcaaagc ggcgacgatt
     2761 gcgaccgaat ccggcgtacc ggtgtatatc tgttcctcgc tcaaacccga tgcacttgcc
     2821 gaagctgccg aacatcaggc ggacggctcg tttttcgtcc cccgtgccaa aggtttgcgg
     2881 acgcagaagc aatggctggc gttctattcc gaaagccggg gcagcgttta tgtggacgaa
     2941 ggtgcggaac acgctttgtc cgaacagggg aaaagcctgc tgatgtcggg cattgccgga
     3001 atcgaagggc atttttcccg tatggacacc gtaaccgtgt acagcaaggc aaccaaacag
     3061 cccctgggca aagggcgcgt cctgttcggt tctgccgccg ccgaagacct gctcaaatcg
     3121 cgtaaggcga aaggcgtgtt catccatcgg gacgactgga tttccatcac gcccgaaata
     3181 cgcctgcttc tgaccgaatt ttagaaaagg aaacccatgt caaacacaca aaaacagctt
     3241 gcccttgcca aagcggcaaa aaaatccgtc aacacggcgg atacggaaga aaaaaaccgc
     3301 gcgctgcttg ctatggcgga cagtctggaa gcggcggcgg cagatatttt ggcggcaaac
     3361 cgtcaagact tggaagccgc ggcaggcaac attcccgaaa gcatgaccga ccgccttttg
     3421 ttggacgaaa aacgcattgg cgcgatggcg gacggcatca gggcggttgc cgcgctgccc
     3481 gaccccgtgg gcgaaatact ggaaacctcg actttgccca acggcttgga aatcgtcaaa
     3541 aagcgcgtgg cgatgggcgt tatcggcatt atttacgaaa gccgcccgaa cgttacttcc
     3601 gatgcggcgg ctttggcact gaaaagcggc agcgcggtcg tactccgcag cggcaaagat
     3661 gcattccaat ccgcacgcgc catcgttgcc gcactcaaag ccggtttggc gcaaacccgc
     3721 atcgaccccg acgcgctcca gttgattgaa gacacagggc gtgagggcag ttacgaaatg
     3781 atgagggcga aagattatct agacctgctg attccgcgcg gcggggcggg gctgatacgg
     3841 gcggtggttg aaaatgccgt cgtgccggtc attgaaacgg gaacgggcat tgtccacatt
     3901 tatatcgaca aggacgcgga ttgggacaag gcgctccgta tcgtgtacaa cgccaaaacc
     3961 agccgtccgt ccgtgtgcaa ctcgatggaa gtgctgctgg tacatgaaga cattgctgcc
     4021 gacttcctgc ccaagctcga acggctgttg gttcgcgacc gcatagaagc cggactgccg
     4081 cccgtccgct tccgtttgga tccgcaggcg gcgcggcata tcggcggcga agcggcgggt
     4141 gcagacgatt tcgataccga gtttttagac tacatcctcg ctgtgaaaac cgtcgcttcg
     4201 gtcgaagagg cggtctggca catcgaaacc cacagcacac accattcaga cggcatcgtt
     4261 accgaaaacc gccacgctgc ggactatttc acgactcata tcgattctgc cgccgtgtat
     4321 gtcaacgcgt ccacgcgctt taccgacggc ggcgaattcg gcttgggttg cgaaatgggc
     4381 atctccacgc aaaaactcca cgcccggggc ccgatgggtt tgaaagagct gacgagttac
     4441 aaatacatcg tacaaggcac gggacaggtc agggaataat ccgaattcaa atgtctgata
     4501 tagtggatta actttaaacc ggtacggcgt tgcctcgcct tagctcaaag agaacgattc
     4561 tctaaggtgc tgaggcacca agtgaatcgg ttccgtacta tttgtactgt ctgcggcttc
     4621 gtcgccttgt cctgattttt gttaatccac tataaaaatg ccgtctgaaa cagtcttcag
     4681 acggcatttc catttgcaaa aaatcaagca ttgtccaagg ggacgagaat cgtacggttg
     4741 ccgttgtgtt ccggtgcgga cacaatgcct tccgcctcca tctggtcaat cagacgcgcg
     4801 gcgcggttgt agccgatacg caaggcgcgc tgtacgcccg aaatgctggc tttgcgcgtt
     4861 ttcaggacaa cggatacggc ctcgtcgtac atcggatcgg tttcgtcgtc gccgctgcgc
     4921 ccgatgccgg gcagctcttc gctgccgccg ccgctcaaaa tatcgtcaac atagtccggt
     4981 tcgccaaact gtttcaaata ttcgaccacg cggtgcacct cttcatccga ggcaaacgcg
     5041 ccgtgaacgc gctgcggata ggcagtaccc ggcagcagga acagcatatc gccctgaccg
     5101 agcaggtttt ccgcgcccat ttggtcgaga atcgtgcggc tgtcgatttt gctggacact
     5161 tggaacgcga tacgcgtcgg gatgttcgcc ttaatcagac ccgtgatgac atcgacgctg
     5221 gggcgttgtg tggcaagaat caaatggatg cctgccgcgc gggctttttg ggcgaggcgg
     5281 gcaatcagtt cttcgatttt cttgcctgcc gtcatcatca ggtcggcaaa ctcatcgacc
     5341 acgaccacga taaacggcag tttttccaaa ggttcgggat cgtcgggcgt gaggctgaac
     5401 ggattgccga ttttttctcc ccttgctgcg gcttcggcga ttttttgatt gaagcccgca
     5461 agattacgca cgcccataaa gctcatcagg cggtagcgtt tttccatttc gttaacacac
     5521 cagttcagcg cgtttgccgc cagcttcata tcggtaacga caggggcgag caggtgcggg
     5581 atgccttcgt aaatgctcaa ttccagcatt ttcggatcga tcataatcat acgcacgtct
     5641 tccggcgcgg ctttgaaaag catagacaga atcatcgcgt tgacacccac cgatttgccc
     5701 gaaccggtcg tgccggcaac cagcaaatgc ggtgctttgc ccaagtcggt tacaacgggc
     5761 tgtccggtga tgtcctgacc gagcgcgagc gtcagcttgg atttggattc ggcaaactcg
     5821 ggcgaattga agatttcgct caggcgtatc atttgacgtt tcgggttcgg aagttccaaa
     5881 cccatgcagg ttttgccggg gatggtttcg acaacgcgga tggaagccac gccgagcgaa
     5941 cgcgccaaat ctttttccag attcagaacg gaattgccgc gcacgccgac atcgggttcg
     6001 atttcataac gcgtaattac ggggccggaa taagaatcga caaccttgac cttgactttg
     6061 aactccgcca atttttcttc gatggtgatg ctgttttcca acagttcttc ttcggtttgc
     6121 gtcgcctcgg gattgaacag cggcggcaga agcaggtcgg ttgtcggcag gtgttcggat
     6181 accgcaccgg tttcttcaga tgggaacgcc ccttcatccg cttccgtatc cgatacccgg
     6241 caggacgggc gttcagacgg cacattttca aacggacaaa ccgcctgact gtcatgcccg
     6301 aacgcttcgg tttccgacag atattgcccg cttgaccgct ctgccgcacc ttcactgccg
     6361 tcatccgcaa tagcggcggt ttcctcctgc caacctccat tcaaaacatc atcggcaaga
     6421 tggtcggtct cggcaatgcg gctgcgttgc acctgctcga atcctgacgg cggttcatag
     6481 gtacggttgt agatttccga tacgggaggc ggcggctgaa tatcgattgc ggtcatggga
     6541 actttcggca cttcgggtgg ttcgaccacc caagcatcgg gcgcggcagg ttcttcaata
     6601 tggatatcgg cagtttcatc ataaaccgga ccgccgtatc ccacagacga aacggtttcc
     6661 gtgaaaacat tttcggtttc agacggcatt tcgacatccc gtttcccgac aaccgtctga
     6721 ctttccggaa tcaggcgaga aattcgcgcc tccgcagaaa ccgttgcctt gttcggttct
     6781 ttcaaattga cggaaacgcg gcggcgggca tcgcggacgg ccgtgccgtc tgaaacggac
     6841 tgcccttggg aaacatccgg ggattttgcc tccgcctgcc ctttcccctg ccctgcatga
     6901 cggtggaacg gcgcattccg tgcattttcg gctttgaccg cagaaaaggc agacggatgc
     6961 tgcttctcaa aatacggttc aaatccgtaa tccgcagact cggaaaacgc ttctttgtcc
     7021 gcatcgaaca tacgggaata agtgcgttga agcacagggt cgtccaaacc gataatctgc
     7081 agcccttcca tcggggtatc ggacacgcgg actttgggga ccgctgtttc gtttttctca
     7141 aatgcatcga tatagcgttt tttcgtttcc cttaaagccg cgctgtttaa agcacgcgtt
     7201 gcttcttcca aagtgatttc tttaaaaacc ggacggacgg gcgaaatttc gctttcagac
     7261 ggcatcaacc cttcagcaat actccggtcg aatgggatgc ggcggttgtc gattacggca
     7321 gttgcaatgt cttccgtatc ggcagcctct tcctccgcag cttctgctgc ttccgtttcc
     7381 gcttcttccg tcccgtttcc actgtctgaa tatccgtcgg atgcggtcgg aacatccgca
     7441 cgatttgcag aagcaggcgg aacgggcctg tccggttcgg attcgtgcgc cagatagttt
     7501 cggcagaaac ggacgacacc gacaaacagc caatacactg ccgttttgac ggcatggaaa
     7561 agcatcaggg caagttcggg aaaatcgggc ataccgtctt tgatttcagg cagctccgcc
     7621 tgtttttccc ctttcttttc ctgccacgca gaaacctcgc gcatccactc gcgttcggat
     7681 tgtgcgcgga caaaaaacaa gccggcaagc gcaagcaaca aaataacgat cataactatc
     7741 caaaacatat gccgtttccc gcaagacaaa cgattaaacg ggtttatttt aacgtgtttc
     7801 catcccaaaa aaaacaaccg gtcggcaatt ctgccgaaac cgattcggct ttcggtttgc
     7861 ccccgccgac agtataatag ccgtcctttc ccttaatctt tgcggcattg ccatgatttt
     7921 ccaaacagtc tggttttcag atatggtact gtcggtttcg tggattgtcc ttatcctgat
     7981 actggcagct tccgcgccgt ccgcattccg ctcgcttgcc cgctaccgtt ccgcccttcc
     8041 cctgtgtacc gtaatttttt ccgcagcatg gtgtctgaac gcctctgccg gcggcggaca
     8101 gcttgcccag atgaactacc acctgctcgc cgtcaatctg gttgcactga tggtgggcac
     8161 ttccgccgcc ttttggcttg ccgcgctgct gatgctgccc tactgcctgc tgtttgccga
     8221 ttctgccggc gcatatccgc cgaacgcttt ggtgctgatt ctgcccgcgc tggtcgtcaa
     8281 ccgcctgtcg cgtatgctgg tcaaccgcct gccgcccaat attttcatct ttattttcgt
     8341 caacggcttt ctcgcttccg ccgccggcat tctgttgacc gggctggtgc tgaccggtat
     8401 tttggatgct gcaaacgctt ttccgtccga aatattgtgg acgaccgccc tgcccgtctt
     8461 tattctgctg gcgtgggcgg aagccttcct cagcggcatt tcaactgcca tatttgttgc
     8521 actgaagccc cattggatta acactttcga cgacaaccgc tatcttaagt ccgaccgcgg
     8581 catatggcgg tagccttcag acggcataga caggcaaaac catgctttcc aacatcatcc
     8641 ccctttccat cggtgcggca ctcggtgcaa cggcgcgttg gctgctcaac ctcgccgttc
     8701 ccgcatcaat acctcccgcc acaggcaacc tgtttgccaa ctggacgggc gcgctgctca
     8761 tcggcatctt tgccgaaacc gtcaaccacc cacaatggaa gctgctgctg atcaccggtt
     8821 ttctcggcag cctgaccacg ctttccggat tttctctgga aaccgtaacc ctgctccaat
     8881 tgaaccgtcc cgcttcagca ctttccaata ttttcctaca cacggcaggt tcgctgctgc
     8941 tgacttggct cgggctgaag ataggggcgg cagtcaaata atcgcaaaaa attgcttcct
     9001 ttggttttga cacaaactat acaagtccta acatcttaat catggcgcag catataagcg
     9061 tggatttgca gcccaatcta tactttctga ttctattcat taagccatta tgcttattcc
     9121 tcaatcacgg tttccaccac cgaatgaata cttttaggca attgcaaaaa agcattccaa
     9181 gcaattaaag cagttttaaa agtatgcagc gaacagctta aatcaggcca gccgtcttct
     9241 tcatcaaatt cgttgt
//
LOCUS       DAXHTY010000075         9071 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXHTY010000075 DAXHTY010000000
VERSION     DAXHTY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.075"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(129..464)
                     /locus_tag="ACFNMK_001555"
     CDS             complement(129..464)
                     /locus_tag="ACFNMK_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6364788.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            593..1498
                     /locus_tag="ACFNMK_001556"
     CDS             593..1498
                     /locus_tag="ACFNMK_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFC6364789.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGNGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1560..2048)
                     /locus_tag="ACFNMK_001557"
     CDS             complement(1560..2048)
                     /locus_tag="ACFNMK_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6364790.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2195..3097
                     /locus_tag="ACFNMK_001558"
     CDS             2195..3097
                     /locus_tag="ACFNMK_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6364791.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3138..4268)
                     /locus_tag="ACFNMK_001559"
     CDS             complement(3138..4268)
                     /locus_tag="ACFNMK_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6364792.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4473..7097
                     /gene="alaS"
                     /locus_tag="ACFNMK_001560"
     CDS             4473..7097
                     /gene="alaS"
                     /locus_tag="ACFNMK_001560"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC6364793.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLSLYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7159..7890
                     /locus_tag="ACFNMK_001561"
     CDS             7159..7890
                     /locus_tag="ACFNMK_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364794.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8317..>9071
                     /locus_tag="ACFNMK_001562"
     CDS             8317..>9071
                     /locus_tag="ACFNMK_001562"
                     /inference="COORDINATES: protein motif:HMM:NF042870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HFC6364795.1"
                     /translation="MDRKNVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTK
                     AKEQAERYLQEIGRTKPSALPEYYAVSDFAHFHLYRRVPEEGVENQWQFPLEALPEYI
                     TRGVFDFMFGIEAKVRQIQEEADIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFL
                     FFADDSAVFQRNYLFQDFLESCKETDTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFE
                     YVNGGLFKERLRTFDFTAKQHRALIDCGNFDWR"
BASE COUNT         2317 a         2390 c         2181 g         2183 t
ORIGIN      
        1 cttacccctt gtttctcaaa tgccgtctga aaaaaacggc tgtatatgtt gtctgcaaaa
       61 tatcgggaag cataacaggc aaaatgccgt ctgaaatatt ttcagacggc atttcaatcc
      121 gattcggatt atttttgcgt ttcaaccgct tccaatgcac gcagggcgta agtgtaagcg
      181 gcaccggcat tcagggcgat ggcggttgcc aatgcgccgg cgatttcgct gtcggtcgca
      241 ccggctttgg cggcggcggc ggcgtgaaca ctgatgcagc tctcacaacg tgtggtaatg
      301 gcaacggcga tggcaatcag ttcgcgcgtt ttggcatcaa gtgcttcggc ggcggcggcc
      361 tgttccaatg cgccgtaggc ctgcagcatt ttaggatgcg ccttacccag ctcgccgaac
      421 gattttttaa ccaatgcggt atgttctttc caatctttaa acattttctt ttcctttctc
      481 ttgcgtttaa ccctgatacg cgcttgcgta tctgttttcg atgtgcgtat tattgcaatt
      541 attcagttgt gtttctcgtt taatcatctc attttatggt tcaaaaagat ttatggacat
      601 tctggacaaa ctggtcgatt tcgcccaatt gacgggcagt gtggatgtgc agtgcctttt
      661 gggcggacaa tggtcggtac ggcatgaaac cttgcaacgc gaaggactgg tacacattgt
      721 tacatcgggc aacggctatc tctgcatcga cggcgaaact tccccgcgtc cggtcagtac
      781 aggggatatt gtatttttcc cgcgcggctt gggtcatgtg ttgagccacg acggaaaatg
      841 cggagaaagt ttacaaccgg atatgcggca gcacggtgcg tttacggtca aacagtgcgg
      901 aaacggacag gatatgagcc tgttttgcgc ccgtttccgc tacgacaccc acgccgattt
      961 gatgaacggg ctgccggaaa ccgtttttct gaacattgcc catccgagtt tacagtatgt
     1021 ggtttcaatg ctgcaactgg aaagcaaaaa acctttgacg gggacggttt ccatggtcaa
     1081 cgcattgtcg tccgtcctgc tggtgcttat cctgcgcgcc tatctcgaac aggataagga
     1141 tgtcgaactc tcgggcgtat tgaaaggttg gcaggacaaa cgtttgggac atttaatcca
     1201 aaaggtgata gacaaaccgg aagacgaatg gaatgtcgac aaaatggtgg cggctgccaa
     1261 tatgtcgcgc gcgcaactga tgcgccgttt caaaagccgg gtcggactca gcccgcacgc
     1321 ctttgtgaac catatccgcc tgcaaaaagg cgcgttgctg ctgaaaaaaa acccggattc
     1381 ggttttgtcg gtcgcactgt cggtaggctt tcagtcggaa acgcacttcg gcaaggcgtt
     1441 caaacggcaa tatcacgttt cgccgggtca ataccggaaa gaaggcgggc aaaaataaat
     1501 cggggcttca aacgcaaatg ccgtctgaaa aggctttcat acagcatttg cgtaccgcgt
     1561 catttcaagg gctgcatctt catcacttcc atcaaaaagt tggtaaatgc ggggttgttg
     1621 ggtttgacat ccatattttt ccaacgctgc tgccagccgc gcaaggcatt ctggatatac
     1681 agcttggact gttccgtatt gattgcgccc cgctggctgt ctatcgccga acgcaggtag
     1741 atttcataca tactgtcatc gacggcattg cgtccgacca ggcgttttct gaagttgttc
     1801 agatattgcg ccgcctgaac cttggtcatt ttaccgatac ccacctgata gcccaagcgc
     1861 gtcgcttcat cgctgatttt ggcaacatcc gtccaatgcg aagaggcaag gcggaaacct
     1921 tttgcaggtg cttccgtttt gacggtattg ataggattca cggggatttc cgtcaatgtg
     1981 ggcacataaa tagactggca gccggaaaga actgccacaa tggaaagagg gataaggtat
     2041 tttttcatgc ccccattata atcaagtttg ccttgagaaa acaaattgtt cggcaagaaa
     2101 aataaaattt cggcatcaga agcaggcaaa aacacattcc acaagccttg ccgcaaggtt
     2161 tacaatccga ccgtccttat cgcaacgacc gtttatggat accgcaaaaa aagacatttt
     2221 aggatcgggc tggatgctgg tggcggcggc ctgctttacc attatgaacg tattgattaa
     2281 agaggcatcg gcaaaatttg ccctcggcag cggcgaattg gtcttttggc gcatgctgtt
     2341 ttcaaccgtt gcgctcgggg ctgccgccgt attgcgtcgg gacaccttcc gcacgcccca
     2401 ttggaaaaac cacttaaacc gcagtatggt cgggacgggg gcgatgctgc tgctgtttta
     2461 cgcggtaacg catctgcctt tggccaccgg cgttaccctg agttacacct cgtcgatttt
     2521 tttggcggta ttttccttcc tgattttgaa agaacggatt tccgtttaca cgcaggcggt
     2581 gctgctcctt ggttttgccg gcgtggtatt gctgcttaat ccctcgttcc gcagcggtca
     2641 ggaaacggcg gcactcgccg ggctggcggg cggcgcgatg tccggctggg cgtatttgaa
     2701 agtgcgcgaa ctgtctttgg cgggcgaacc cggctggcgc gtcgtgtttt acctttccgt
     2761 gacaggtgtg gcgatgtcgt cggtttgggc gacgctgacc ggctggcaca ccctgtcctt
     2821 tccatcggca gtttatctgt cgtgcatcgg cgtgtccgcg ctgattgccc aactgtcgat
     2881 gacgcgcgcc tacaaagtcg gcgacaaatt cacggttgcc tcgctttcct atatgaccgt
     2941 cgttttttcc gccctgtctg ccgcattttt tctgggcgaa gagcttttct ggcaggaaat
     3001 actcggtatg tgcatcatca tcctcagcgg tattttgagc agcatccgcc ccactgcctt
     3061 caaacagcgg ctgcaatccc tgttccgcca aagataaaaa atgccgtccg aacatccttc
     3121 agacggcata tcgggcttta tttccccgcc ttcacatcct gccactggcg caccataaac
     3181 ttcaatgccg ccggctggat aggcaccatg ataaagctgt ttttcaaatc ctcctcggtt
     3241 gggaaaatcg tattgtcgtt tttaaattcg tcttccatca gctcacgcgc cggcttgctc
     3301 gaaggcgcat aagtaacgaa attgccgttt ttcgccgaca cttccgggtc gaggaagtcg
     3361 ttgatgtatt tgtgcgcgtt ggcgacgttt ttcgcatctt tcggaatcac gaaagaatcc
     3421 acccaaatcc ccacgccctc tttgggcatc atcacgcgga ttttttcctt gccgcccgct
     3481 tcttcggcac ggcgtttggc gatgttcaaa tcgccgccga aaccgattgt tacgcaggta
     3541 tcgccgcgcg ccaaatcatc gataaagccg gacgaagtga agcgtttgat attggggcgg
     3601 tttttcttga gcagggcggt tgcctccctg atgtcttccg tattgctgct gttcgggttt
     3661 ttacccaaat agttcaacac cataggatag atttccgccg cgctgtccaa atagctgatg
     3721 ccgcattgct tgagtttgga cgtgtattcg gggtcgaaca ccaaatccca ctggttgtcc
     3781 ggcagcttgt ccgtacccaa agcctttttc acgcgttcgg tattgatggc gaaggtattt
     3841 gtcccccaat aaaacggcac ggcgtattcg tggccgggat cgaccccgtc catcagcctc
     3901 atcatttcag ggttgaggtg tttataattg ggaatcagcg acttatcgat tttctgatac
     3961 gcacctgcct taatctgcct gcccacaaac gcattggacg gcgcgacaat gtcgtaaccg
     4021 gattttccgg tcagcacctt gctttccagc gtttcatcgc tgtcgtacac atcataagta
     4081 accttgatgc cgtttttctt ttcaaaatcg gcaacggttt ccggatcgac gtattccgac
     4141 cagttgtaaa ttttcaatac gtttcggttt tccgccggcg ccggtttttc ggcaggcggt
     4201 ttgtccgaac cgccgcacgc tgcaagcagc aaagcagtca ggacggcgag tggcagatgt
     4261 ttggccatta tcattccttg catatcgggt tggagaaagc ggccattata gccgatattg
     4321 gcaacagggc ttcagacggc attcaaaatc ccgccacact cttccgaaaa ccgccgcttc
     4381 catagctaga aacagggatt tgcggtaaga taccgccgtt cgttttccct gcttttacca
     4441 tgacacgaca tttgagagac attgaaaaaa ttatgaaaac ctccgaactg cgccaaaaat
     4501 tcctaaaatt ttttgaaacc aaaggccaca ccgtcgtccg ctcttccagc ctcgtgccgc
     4561 acgacgaccc gaccctgctg tttaccaacg cgggcatgaa ccaatttaaa gacgtattct
     4621 taggtttcga caaacgcccg tacagccgcg ccaccaccgc gcaaaaatgc gtacgcgccg
     4681 gcggcaaaca caacgacttg gaaaacgtcg gctacaccgc ccgccaccac accttctttg
     4741 aaatgatggg caacttctcc ttcggcgact acttcaaacg cgacgccatc catttcgctt
     4801 gggagtttct gacttccccc gaatggctca acatccctaa agacaaactg ttggcgaccg
     4861 tttacgcaga agacgacgaa gcctacaaca tctggttgaa cgaaatcggt atgccgtctg
     4921 aacgcatcgt ccgcatcggc gacaacaaag gcgcgaaata cgcgtccgac aacttctggc
     4981 aaatgggcga caccggccct tgcggcccct gctccgaaat tttctacgac cacggcgaag
     5041 aaatttgggg cggcattccc ggcagccccg aagaagacgg cgaccgctgg atcgaaattt
     5101 ggaactgcgt attcatgcag ttcaaccgcg acgaacaagg caatatgaat ccgctgccca
     5161 agccgtccgt cgataccggc atgggcttgg aacgcatggc cgccgtcatg cagcatgttc
     5221 acagcaacta cgaaatcgac ttgttccaag acctgctcaa agccgttgcc cgcgaaaccg
     5281 gcgcagcttt cagcatggaa gaaccaagcc taaaagtcat cgccgaccac atccgctcct
     5341 gctcattcct gattgcagac ggcgtattgc cttccaacga aggccgcggc tacgtattgc
     5401 gccgcattat ccgccgcgcc gtgcgccacg gttacaaact gggtcaaagc aaaccgttct
     5461 tccacaaact cgttgccgat ttggtcaaag agatgggcgg tgcctaccct gagttgaaag
     5521 agaaacaagc acaaatcgaa gaagcgttga aaaacgaaga aagccgtttt gcccaaacgc
     5581 tggaaactgg tatggctttg ttggaaaacg cgctggccaa aggcggcaaa acactcgacg
     5641 gcgaaatcat cttcaaactc tacgatactt acggtttccc atacgatttg accgccgaca
     5701 tctgccgcga acgcaatatc gaactggacg aagcaggctt cgagcgcgaa atggaagccc
     5761 aacgcgcacg cgcacgcgcc gcacaaagct tcaaagccaa cgcccaactg ccttacgacg
     5821 gtcaagatac cgagtttaaa ggttatagcg aacgccaaac cgaatccaaa gtcctctcct
     5881 tatacaaaga cggcgagcaa gtcaacgaat tgaacgaagg cgacagcggc gcagtcgtca
     5941 tcgactttac cccgttctat gcagaatccg gcggccaagt cggcgatgtc ggctatatct
     6001 tctcaggcga aaaccgcttt gaagtacgcg atacccaaaa aatcaaagcg gccgtattcg
     6061 gtcaattcgg cgtacaaact tcaggccgtc tgaaagtcgg cgacagcgtt accgccaaag
     6121 tagatgacga aatccgcaat gccaatatgc gcaaccacag cgcgacccac ctgatgcaca
     6181 aagccctgcg cgacgtattg ggcggacacg tcgaacaaaa aggctctttg gttaccgccg
     6241 aatccacccg tttcgacatt tcccatcccc aagcggtaac tgccgaagaa atcgccgaag
     6301 tcgaacgccg agtcaacgaa gccatcttgg ccaacgttgc cgtcaacgca gccattatga
     6361 gcatggaaga tgcccaaaaa accggcgcca tgatgctctt cggcgaaaaa tacggcgacg
     6421 aagtgcgcgt actgcaaatg ggcggtttct ccaccgaatt gtgtggcggt acacacgttt
     6481 cacgcaccgg cgacatcggc ctcttcaaaa tcatcagcga aggcggtatt gccgcaggcg
     6541 tgcgccgtat cgaagccatc accggcctga acgcactcaa atgggcgcaa gagcaagagc
     6601 gtttggtgaa agacatcatt gctgaaacca aagcccaaac cgaaaaagac gtactggcga
     6661 aaatccaagc aggcgcggca cacgccaaag cattggaaaa agaattggca cgcgccaaag
     6721 ccgaactcgc cgtccacgca ggcgcaaaac tcttggacga cgcaaaagac ttgggcgcag
     6781 ccaaactcgt tgccgcccaa atcgaagccg acgcagccgc cctgcgcgaa atcgtaaccg
     6841 acctgaccgg aaaatccgac aacgccgtga ttcttttagc ggcagtaaac gacggcaaag
     6901 tctccctgtg cgccggcgta tccaaaccgt tgaccggcaa agtgaaagca ggcgatctgg
     6961 ttaaatttgc agccgaacaa gtcggcggca aaggaggcgg ccgtcccgac ttggcgcagg
     7021 ccggcggcac ggatgccggc aaattgcccg aaatgctggt aagcgtggaa agctggcttt
     7081 gtcagaaatt aagttaaatt taaatcttaa taagccattt gagtcttgtt caaatggctt
     7141 atattttgga ttgttagaat gaatactaaa acagagttac aaaaactttt agaagaggat
     7201 atatcaactt taacagaaac actaatttgt gcagatgctc ttcctccaag atatgtacgt
     7261 agcattgcaa ctcctattgt ccgtcgatgg ttaattgaca aacaattaaa tattctcgca
     7321 aaagaaatag gtttaactat agaattgccc atccttgata caagtttggt atttgaaaaa
     7381 ttgtcaacct tagagaataa agttaatttt tatatggctg gtggagttta tttaggcgga
     7441 gaatttatta gctctatata tcattcatca caagaatttt ctggtgaacc aataatctat
     7501 gctgaaccaa atataatttt atgtcctgct gaaaaatttt taactttaaa acgagttttc
     7561 cataatggaa atatttttaa tatgaatcag ataattactt ttttatccaa taaacaagga
     7621 ggagtacatt ttgataaaaa ttacgataaa tataaaactt ggcaagttgc tatagaaaaa
     7681 gcagcaaatt ttttaaaact cggtaatcca tataatgaag ataaattatc tcttagtgaa
     7741 gaacatgata ctattttagt agttttacca ttagaaaaag gatatgaatg gaattgtctt
     7801 gagatagaag tactatccgc tgctcaatct ctagcaaata tctattgcaa caaagttcgc
     7861 ctcatagatg gtcatgtttg gaaggaataa aagaaaaagc atagttggta atttttagtt
     7921 acgtagggtg ggcttcagcc caccgttttt tcatttttga ttgttgattg gtgggctaaa
     7981 gcccacccta cggttactaa aatcccgtca ttacctcgca ggtgggaatc cggtttgttc
     8041 ggtttcggtt ttttagggac agtgccgcaa cattaaattt ccagattccc actttcgcac
     8101 aaataacagc gcaaataggg caaagataag gatatggggt tttttctttg aatttactat
     8161 acaattcgcc cgtccccgtt atagaaaccc aagccgatga aaaccctgct ccaactccaa
     8221 accgccgcac aaaacttcgc cgcctactac aaagaccaaa ccgacgaacg ccgcgagaaa
     8281 gacaccttct agaacgaatt tttcgccatt ttcggcatcg accgcaaaaa cgtcgcccac
     8341 ttcgaatacc ccgtcaaaga ccctgccgac aacacccaat tcgtcgatat attttgggaa
     8401 ggcatcttcc ttgccgaaca caaatccgcc aacaaaaacc tgaccaaggc caaagagcag
     8461 gcggagcgtt atttgcagga aatcgggcgc accaagccct ctgcgctgcc cgaatattac
     8521 gccgtcagcg attttgccca tttccacctt tatcgccgcg tacctgaaga aggcgtggaa
     8581 aaccaatggc agttcccttt ggaagcattg cccgaataca tcacgcgcgg cgttttcgac
     8641 ttcatgttcg gcatcgaagc caaagtccgc caaattcaag aagaagccga tattcaagcg
     8701 gcggcggcca tcggcaggct gcacgacgcg ctcaaagaag aaggcattta cgaagaacac
     8761 gagctgcgcc tcttcatcac gcgcctgctt ttcctctttt ttgccgacga cagcgccgtt
     8821 ttccagcgca actatctctt tcaagacttt ttagaaagct gcaaagaaac cgacacgctc
     8881 ggcgacaagc tcaaccaact ctttgaattc ctcaacacac ccgaccaaaa gcgcagcaag
     8941 acccaaagcg aaaaatttaa aggtttcgaa tacgtcaacg gcggcctgtt taaagaacgc
     9001 ctgcgcacct tcgacttcac cgccaagcag caccgcgcct taatcgactg cggcaatttc
     9061 gactggcgca a
//
LOCUS       DAXHTY010000076         9071 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXHTY010000076 DAXHTY010000000
VERSION     DAXHTY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.076"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(4..1260)
                     /locus_tag="ACFNMK_001563"
     CDS             complement(4..1260)
                     /locus_tag="ACFNMK_001563"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC6364796.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLQNLKIDL
                     DTGKYLRLDSAKHQTDGFFAAVLQRK"
     gene            complement(1608..1949)
                     /locus_tag="ACFNMK_001564"
     CDS             complement(1608..1949)
                     /locus_tag="ACFNMK_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364797.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEVDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2153..3010)
                     /locus_tag="ACFNMK_001565"
     CDS             complement(2153..3010)
                     /locus_tag="ACFNMK_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC6364798.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPQGFNAFEPPLNPYGWRGIYIQY
                     WAEYGFERQEARYHLLDRMAYLAPARVAETEALNETEIRRIDQGLIAVLELRPDGFWH
                     EIERIMKAV"
     gene            complement(3071..3886)
                     /locus_tag="ACFNMK_001566"
     CDS             complement(3071..3886)
                     /locus_tag="ACFNMK_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC6364799.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            3950..4120
                     /locus_tag="ACFNMK_001567"
     CDS             3950..4120
                     /locus_tag="ACFNMK_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364800.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCTFELAVCHP"
     gene            4130..4915
                     /locus_tag="ACFNMK_001568"
     CDS             4130..4915
                     /locus_tag="ACFNMK_001568"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6364801.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLSKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDAWLESGDIVAANPKVLAQMLKIISAHV"
     gene            5184..7303
                     /locus_tag="ACFNMK_001569"
                     /pseudo
     CDS             5184..7303
                     /locus_tag="ACFNMK_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            7427..8977
                     /locus_tag="ACFNMK_001570"
     CDS             7427..8977
                     /locus_tag="ACFNMK_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC6364802.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         2281 a         2164 c         2380 g         2246 t
ORIGIN      
        1 ggtttatttg cgttgcaata cggcggcgaa gaagccgtcg gtttggtgct ttgccgaatc
       61 gaggcgcagg tattttccgg tatccaaatc aatcttcagg ttttgcagca gttcggcgca
      121 gttgacgggt tcaaattcgg gatgttcgga caggaaacgt tcgacttgca gctcgttttc
      181 ttcgggcagg atgctgcaag tggcgtaaac caagcggcct tgcggtttta ccagtttgga
      241 ggcagcatcg aggatgctgt gttgctgttc caaaaggttg gccacggtct cggcagattg
      301 gcggtatttg aggtcgggat tgcggcgtaa agtacccaaa ccggagcagg gcgcgtccac
      361 caacacacgg tcggctttgc ctgccagtcg ggcgatacgg ctgtcgtgtt cgctgctgat
      421 gcgttcgggg tggatattgg tcagtccggc gcgggtcata cgcggtttga ggttggcaag
      481 gcgtttttcg gcgatgtcga aggcgtagat tctgccttta ttggccattt gcgcaccgac
      541 agccaaggtt ttgccgccgg caccggcaca gaaatcgaca atgatttcgc cgcgttttgc
      601 gcccaccaat aaagcaagca gctggctgcc ttcgtcttga acttccagcg taccgtccaa
      661 aaacagctcg tgtttgttaa gcgcgatttt gtttttcagg cggatgcccc aaggcgaata
      721 aggcgttgcc tctgcatcgg cactttcggc ttgcaacaat ggcagcactt tatcgcgttt
      781 gcctttcaag gtattgacgc gtatatcgag cggcgcagct tggttgatgc tgcggccgaa
      841 ggcgaggatt tcttcttcgc gccaatgctg ctgcagctgt tctaccagcc attgcggcaa
      901 ttctgcggcg gtattcaggc cgtctgaaaa ctcggtttta cgggctttca aattgccgag
      961 aaactctatt tcttcttcat caagcaggtc tttgatttgg ctgatgttgg tgcttctgcc
     1021 gagaaccagt gcggcgagtg cggctttgcg cggctgcgcg tgcggacggc gcagggcggt
     1081 actgattttt tgatagtggc gcagcgcggc aaaggcggtt tcggcgattt cgtggcggtc
     1141 ttggcggccg agctttttgt gttcgcggaa ataggcggag aggacggcat cggaaggctg
     1201 tttgaaagtc agcatttcag ccaaaacttt ggcggtatgg tcgagttgtg cggcgttcat
     1261 aatgtaatgg gttctcttac tgtgggttat tcataatcaa tctaacgtcc ttaaaataac
     1321 aatagcatct ttatcattac ttagtattct atccgcaata agaaagatgt gttaaaagtg
     1381 cgcaatcggg atttgtctaa atttctccgt taactacaaa ggcagcctga aaaaagttct
     1441 attttcaggt cgcttgaaca gcatccgata taatcacatc agcgatttca ttttgtaatc
     1501 actccccaaa acatgaggaa aaaaacaaaa tttattttct tcgttcagca tatctatcct
     1561 aaatatgcca tcactccatt gagtttttaa tttgtatttc gctcttgtta aatgtttttc
     1621 tgtttgccga tttcttttgc taatgtaaaa aatactctac cactctcatt ggttaaagtt
     1681 ttcaagtatt catcaacatc agtaacatta ataacaactg tttcatttgt atgtttatta
     1741 aattctaaat caataataat attcccatta accaatattt catatatatc tacttcttca
     1801 ggcagcatag acaaacaata agcacttgaa gctgaaaact cttttttcag atactgaaga
     1861 agaaaagtat ttttaattaa aatattatta aatttatcta atgattgctt gacttcttta
     1921 gttgtccaat ttttaacatt attcttcatt gtattctacc tggggtcgta gttattaaat
     1981 taccatactt atttgtttgg atcgtcataa tattagttgg catgttgttg ttatatttat
     2041 catatccaat atccttatta actcggatga ctctttcata aattgtatgg gaaatgagat
     2101 ttccatcact aagcctctca atagatatac gcgttaatgt tgggaaaggg tttcagacgg
     2161 ccttcataat gcgttcgatt tcgtgccaaa agccgtcggg gcgcaattcc aaaacagcaa
     2221 tcaagccttg gtcgatacgg cggatttcgg tttcgttcaa tgcttcggtt tcggcaacac
     2281 gcgcaggcgc gagataggcc atacggtcga gcaggtggta gcgtgcttct tggcgttcaa
     2341 acccatattc cgcccaatat tgaatataga taccgcgcca gccgtatgga ttaagcggcg
     2401 gctcaaaggc gttgaaacct tgcggaaaaa atccgatgcc gcgcacgatg gaaacgggtt
     2461 ttacgtcaag cggcagaccg tgttgccgca aggttcggat gccgtctgaa gtgtgcggca
     2521 gaccgagttg ggcggtcagt ttggcggctt ttcccaacag cgtgtctttg gggtcgagtc
     2581 cgcgcatgtc ttcgagcctg cccgtgccgc cgccgtaata tttgcaggtc agctcgatat
     2641 ggtagggttt gccgttaagc cttaccacaa aatccgccgc gccttgcgta ttgccgtccg
     2701 aaccggaaac cgtgaggttg tgcgcgagca gttcggcgtg tggcgcgttg gcaaaccaaa
     2761 atgccagcag ttcttcggcg taaatgccga ggcggtgtcc gaacggggcg cgtcgggtga
     2821 ggtgttccgt cagcggcgcg ggatcggcat ccaatgccaa aagatagcgg aaaccgcgtt
     2881 cccccaatag ttcgcgcacg cccaattcgc aaccgctttg ccacaaaggc ggcgcagtca
     2941 gcagcgaggc gaggtcgcgg acgggttggc tggtgagctt ccaccataat gcgtctaggg
     3001 cgtaattcat gcttatccga atatcagtca tgccgtctga aaagctggaa atgttttcag
     3061 acggccttaa tcagtctttt ttatggatac gatggctgac ggtattgaaa aaaccgcgca
     3121 ggatatcgat gtcttcggtt tgcgtgttgg cgcgtccgaa caggctctgc atacggcgca
     3181 tcagacgctc gccgttgcgg cggttgaaaa agccgatgtc gttcatcacg ctttccatgt
     3241 gggcgaccat gcctttgatt tgctcgtggg tcgcagcgtg gtcttcttgt tgaagatggg
     3301 tcatgggcga accggtttgg ctgaagattt cgtagcacac gacctgcacg gcttgggcga
     3361 ggttgagcga gaaatagtcg ggattgccgt tgatggtcat cagtcggttg caggcttgga
     3421 cttcttcgat gctcaagccg aaagtctcgt tgccgaaaac cagtgccact ttctcgcctc
     3481 ggtttgcggt ctgcagtaat tcggatacca aatcgcgcgg ggtttgcagc ggcgcagtaa
     3541 tttcgcggcg gcggctggtc agggcgcagg cgatggtggt gtcagcaagg gcttcgtcca
     3601 aagaagcggc aatggtggca ttttccaaaa cgtctgccgc gccggaagcg aggatgaagc
     3661 tttcttccgg taatttaaac gattgaggat gctccgggtc aaacacgggc gggttttccg
     3721 tcatcggcgt tgccatcaga tttggggcga cgatggtcag tttgtgcaga cccattgttt
     3781 tcatcgcgcg cgcggcagag ccgatgttgg cgggatggct ggtgcgcgtg aggatgatgc
     3841 ggatgttgtc cagataagcg ggcagggcgg gtttgagagt agtcatgttc ggttttcggt
     3901 cggaatggaa tagggtataa tgcgccgtct ttttcgttcg gacggtaaaa tgccgtctga
     3961 aatgttcttt aacaacgtcg gaaatttaac gttgcgcctg aagcagacac gcgtcgggcg
     4021 gtattttacc cgatttggca ggatttaccc caaccaaata aactccgatt ttgccgctcc
     4081 gaaggactgt acgttcgaac tggcggtatg ccacccttaa ggaaatctga tgaatccgtt
     4141 tttgaataca gcctttaaag ccgcccgccg tgccggtcag atgatgatac gcgccgcagg
     4201 taacctcgat gccgtcaaaa ccgacagcaa agccttcaac gattttgttt ccgatgttga
     4261 ccgcaattcc gaaatcatcc tggttgaggc tttgaaagaa gcctatccgc accacaaaat
     4321 cacttgtgaa gaaagcggct cccacggcaa agccgcagcc gagtacgaat ggattatcga
     4381 tccgctcgac ggcacgacca atttccttca cggtcatccc caatacgcca tctctatggc
     4441 gctgctgcac aaaggcgtgt tgcaagaagc tttggtttac gcccccgaac gcaacgacgt
     4501 atatatggct tcgcgcggca aaggcgcgtt gctcaacgac cgccgcatcc gcgtttccaa
     4561 ccgcatcgaa ttgaaccgct gcctgatcgg taccggcttc cctgttgtcg atcaaagcat
     4621 gatggacaag tatctggcga ttttgaaaga tttcttgtca aaaaccgccg gcggccgtcg
     4681 tgaaggcgcg gcttctttgg atttgtgtgc tgtagcaaca ggtcgttttg acggcttttt
     4741 cgagtttaac ctcaaaccgt gggacattgc cgcaggtgca ttgattgttc aggaagcggg
     4801 cggtatcgta accgatatga cgggtgagga cgcttggttg gaaagcggcg atattgtcgc
     4861 cgccaatccc aaagtattgg cgcagatgct gaaaatcatt tccgcacacg tttaaatttc
     4921 agccctttcc cgaaaatatg ggaaaacggg cgggtatgcc gtctgaagta tcgcgccgat
     4981 atttcagacg gcatttttat tggcaacgcg gcaggacggc ggaaacggtc aaatgcccca
     5041 aactgccgag gatggaaacc catatctgat taaacggaga accctgtgcg atttgtttat
     5101 agtggattaa caaaaaccag tacggtgttg cctcgcctta gctcaaagag aacgattctc
     5161 taaggtgctg aagcaccaag tgaatgggca aagccaacgc ctttaccgca ccgattaccg
     5221 tcgtcaatta cgacgaacaa gccctcaata acaccgaagc gcgtactttg gtggatgccg
     5281 tagcgaaaaa agacgcttcc gtttggcagt tcggcggcga aagcaacacg ctgaccggac
     5341 tgtatttcag aggttatcag cttgatgcgc gccaattcag cgtcaacggt ttggcaggta
     5401 tgtacggcac gcaaggcacg gcaagcgtgc aagttggatc ggcacaactg attaaaggcg
     5461 catccactgc cgtaaacggc atggaccctg aaggtgcggt atccggttcc gtcaatatcg
     5521 aaaccaaaaa agccgccgat gaaggcaacc gcaaaatcgg tttgggctgg ttcagtaaca
     5581 accgcgccca aggtacgttc gacttgggcc aacgtttcgg cgaaaacaaa gcattcggcg
     5641 tgcgcgccaa cggcaaactg cgccacggcg acaccccgcg ccacggttac agcgaagaca
     5701 acaaagaatt tgcggtaaat gccgactatc gcggcgaaac actgcgcgta gcgttcgatt
     5761 ccatctacgc aaaacgcaaa accaacggcg gtcgcgcgcg tatgcaggac attcaaaacg
     5821 ccggcggacg cttgtttggc gcgcccgatg gtaaaaccaa cctgctgcct tcttggaact
     5881 ggcaaaatac cgccggccaa accaatatgc ttacgtttga atgggatgcg tttgataatg
     5941 cacagattac aggcggtatc ggctacaaca aggcacgcta ttacggcacg ctgatttccc
     6001 cgaccgtatg tttgaatgct accagcactt gtaccgatat gcaaaacaca aacgccagcg
     6061 gcaaagtaac ggcacgcaac tatgactaca ataccggtac tgcgcgcctg accgaccaat
     6121 atttccgcac attgagtatg aacctgaccg cacgcggcga atttgaaacc ggcccggtaa
     6181 cacataactg gagtatggct ttcgaccgcg ttatccgcca gcggaaaacc ataaacggca
     6241 gcgggaacgg caacagcaag atagaagtga aggcaaacga aaatattgcg aatcaattgg
     6301 catcgtttac agccgactat cccaattctt gggagaactc tgccaacttg gatgccaata
     6361 tcaaagtcaa cagcctggct ttgtccgaca ccttgggctt cgtggataac aaataccgcc
     6421 tgactttggg cgaacgtttc caaacagtcg aatataccga caaaaaatca cagagcggcg
     6481 atgccaaacg tttcagcccg atgctgatgg cggcgtgggt gccgcaacct gatttggttg
     6541 tgtacggcaa ctatatggaa gacttggagc ctgccgacat caaaaccgat gattccggcg
     6601 aaacgacaat ggcgaaaccg cgtgtcagcc gccagtttga agtgggcgtg cgtaaaaact
     6661 ggggcaattt cgtaaccacg ttaaacgcgt tccaaatcaa acgcccggct actggcgcgg
     6721 tcatacaact accaagacaa caaaaggggt aacaacaacg ctgacctatg gcaacaattc
     6781 cgatttcgcc cgctataagg cgcaaggcgg cgtagccggc gacgagcaag gtatggaacg
     6841 caatcgcggt atcgagttca atgcctatgc taacctgtta aacaaaaccc tgcgtccgac
     6901 tttgggcctg atgtatctgc aatcgaccgt gaaagagtat ccaaattcac gcgatatgct
     6961 ggttaacggc gtacaagtcg ccaatccgcg cgtaattgcc aaggcaggtt tggaatggga
     7021 tacaccgttt gccaaaggct tgactttaaa cagcaatgtt tcgtatttcg gcaagtctta
     7081 ccaagacacg caaaaacaat acgccttccc atcctatacc ttggttgatg taggcgcgcg
     7141 ctacaaaacc aagctcggca aaaatacgtt gaccgtcagc agctcggtgg aaaatctgtt
     7201 caacaaaaac tactggcagg tacagcgcgg ccaatacgac cgcagcttcg ccgtcgtcgg
     7261 tctgccgcgt acttactggc tgaaggcgga attggatttc tgatttctcc gcatctggaa
     7321 aatgccgtcc gaacaggttt cagacggcat ttcttttaaa gagatatggc gggatacgct
     7381 taaataatgc tattctagcg tctttgaaac ctgcttgttt gaagtgatga aaaaaccttt
     7441 gatttcggtt gcggcagcat tgctcggcgt tgctttgggc acgccttatt atttgggtgt
     7501 caaagccgaa gaaagcttga cgcagcagca aaaaatattg caggaaacgg gcttcttgac
     7561 cgtcgaatcg caccaatatg agcgcggctg gtttacctct atggaaacga cggtcatccg
     7621 tctgaaaccc gagttgctga ataatgcccg aaaatacctg ccggataacc tgaaaacagt
     7681 gttggaacag ccggttacgc tggttaacca tatcacgcac ggcccttttg ccggcggatt
     7741 tggcacgcag gcgcacattg aaaccgagtt caaatacgcg cctgaaacgg aaaaagttct
     7801 ggaacgcttt tttggaaaac aagctccggt ttcccttgcc aataccgttt attttaacgg
     7861 cagcggtaaa atggaagtca gtgttcccgc cttcgattat gaagagctgt cgggcatcag
     7921 gctgcactgg gaaggattga cgggaaaaac ggtttatcaa aaaggtttca aaagctaccg
     7981 caacagctat gatgcgccct tgttcaaaat caagctggca gacaaaggcg atgccgcgtt
     8041 tgaaaaagcg catttcgatt cggaaacttc agacggcatc aatccgcttg ctttgggcag
     8101 cagcaatctg accttggaaa aattctccct agaatggaaa gagggtgtcg attacaacgt
     8161 caagttaaac gaactggtca atcttgttac cgatttgcag attggcgcgt ttatcaatcc
     8221 caacggcagc atcgcacctt ccaaaatcga agtcggcaaa ctggcttttt caaccaagac
     8281 cggggaatca ggcgcgttta tcgacagtga agggcagttc cgttttggca cactggttta
     8341 cggcgatgaa aaatacggcc cgctggatat tcatatcgct gccgaacacc tcgatgcttc
     8401 tgccttaacc gtattgaaac gcaagtttgc acaaatttct gccaaaaaaa tgactgagga
     8461 acaaatccgc aatgatttga ttgccgccgt caaaggcgag gcttctggac tgttcaccaa
     8521 caatcccgta ttggacatta aaacttttcg tttcacgctg ccgtcaggaa aaatcgatgt
     8581 gggcggaaaa atcatgttta aagatttgaa gaaggaagat ttgaaccaat tgggtttgat
     8641 gctgaagaaa accgaggcag acatcagaat gagtattcct caaaaaatgt tggaagactt
     8701 ggcggtcagt caggcaggca atattttcag cgtcaatgcc gaagatgagg cggaaggcag
     8761 ggcaagtctt gacgacatca acgagacctt gcgcctgatg gtggacagta cggttcagag
     8821 tatggcaagg gaaaaatatc tgactttgaa cggcgaccag attgatactg ccatttctct
     8881 gaaaaacaat cagttgaaat tgaacggtaa aacgttgcaa aacgaaccgg agccggattt
     8941 tgatgaaggc ggtatggttt cagagccgca gcagtaatgg gtcaaatgcc gtctgaaatg
     9001 ttcaggcggc attttgttgt attggaaact gccgttttca accattcctt tagataagcc
     9061 gtgtcggatt c
//
LOCUS       DAXHTY010000077         8977 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXHTY010000077 DAXHTY010000000
VERSION     DAXHTY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.077"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(344..556)
                     /locus_tag="ACFNMK_001571"
     CDS             complement(344..556)
                     /locus_tag="ACFNMK_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC6364803.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            complement(568..849)
                     /locus_tag="ACFNMK_001572"
     CDS             complement(568..849)
                     /locus_tag="ACFNMK_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364804.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGRHYRHSENGITLYDFIFFDGKPADEARFAAILTETCRRAVKKICETK"
     gene            complement(887..1090)
                     /locus_tag="ACFNMK_001573"
     CDS             complement(887..1090)
                     /locus_tag="ACFNMK_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364805.1"
                     /translation="MNIIEQETKRAELRKIDAEIAKIIADAHKINAESVKIAQESRWY
                     PMIAATGLVTAIAAVLALIFKFA"
     gene            complement(1177..1470)
                     /locus_tag="ACFNMK_001574"
     CDS             complement(1177..1470)
                     /locus_tag="ACFNMK_001574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364806.1"
                     /translation="MHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHH
                     YLPPTATASKSGANSPNGTAAATAKHTKPPPKPRKTKNRLKKIQTASCPKHLQ"
     gene            complement(1502..1795)
                     /locus_tag="ACFNMK_001575"
     CDS             complement(1502..1795)
                     /locus_tag="ACFNMK_001575"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC6364807.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPPSRPHQHQIRPQRHPTTGL"
     gene            complement(1770..2114)
                     /locus_tag="ACFNMK_001576"
                     /pseudo
     CDS             complement(1770..2114)
                     /locus_tag="ACFNMK_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2107..2364)
                     /locus_tag="ACFNMK_001577"
                     /pseudo
     CDS             complement(2107..2364)
                     /locus_tag="ACFNMK_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2375..2695
                     /locus_tag="ACFNMK_001578"
     CDS             2375..2695
                     /locus_tag="ACFNMK_001578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364808.1"
                     /translation="MLGVLDCYQKSQSCPNPKSHCCFVSDDIFLWYGSRPTFEISLDL
                     TGKKKETSSAKKFVMPCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENF
                     RQYE"
     gene            2688..2882
                     /locus_tag="ACFNMK_001579"
     CDS             2688..2882
                     /locus_tag="ACFNMK_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6364809.1"
                     /translation="MNNYYLYRNCSSDVLWVKRIQRQIDGSLLLISDNSTYPPMPLAL
                     AEHPDIQIIGQVVQVSKDLN"
     gene            2906..3466
                     /locus_tag="ACFNMK_001580"
     CDS             2906..3466
                     /locus_tag="ACFNMK_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364810.1"
                     /translation="MKILALLIAATCALSACGGQAEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSVANKGLADQKTGLTLPEHVVPADNAEGKNLLHDFSDGLTLTVDTDKADKITA
                     VRVVWNTDAVPQKAEKLSKAAAALIAATAPGDRTLLRDTGDQIKMAIDSHNAQKEPTR
                     EWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            3607..4665
                     /locus_tag="ACFNMK_001581"
     CDS             3607..4665
                     /locus_tag="ACFNMK_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364811.1"
                     /translation="MKNKLTLRYFKITRCGYWSGSSKEPNHYEDLLKTLTHLETYVKG
                     KTIEETGISRDDKEPSTFLLDICKKNGLWLLAFWNPAAESSSDEIVSLNMKSKLGQVD
                     SSPVTAGNGKTFGFVSYFLIWPEHNFYSTILSPNATLAGRTEFEEYMLNFLWIDPNFI
                     EKTTYEETSTDGIIHTMERTNFKLPLKINGTPVPRFAGKLAKSPSEKEYVISNYDQVT
                     QVHNQCTVKVTSGQSSSGILKKMLPSFFGSTELFAPLKTIRVNHSVSAAFENKSNLKD
                     WIEDWEKNSFDLDKDDCGFKLKNDDKIYWIRGNVIKCELDVNLHKENGIYPAAKILDI
                     IDQRKDYVLNEFKLSDDS"
     gene            4655..5161
                     /locus_tag="ACFNMK_001582"
     CDS             4655..5161
                     /locus_tag="ACFNMK_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364812.1"
                     /translation="MILRNIFLICSAFMIGVAWYLGANIRFEEQWVLYEALRTTASII
                     FAVVGVWLAIVYPERLKSPFSQTFSSPVYRRGFKQLFSPVISSIFILSIVLLVGLLAP
                     LLKHIEAIIQYIPLCRKISFSLLASLTMWQIYTVLSVLVPLLNILSKNENDCHASNII
                     NKLKGEQN"
     gene            5265..5768
                     /locus_tag="ACFNMK_001583"
     CDS             5265..5768
                     /locus_tag="ACFNMK_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFC6364813.1"
                     /translation="MSTKFNQFINRVLSHEGGYVNHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAVRMLQRAAG
                     VADDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLSTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            5882..6217
                     /locus_tag="ACFNMK_001584"
     CDS             5882..6217
                     /locus_tag="ACFNMK_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364814.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAAVPQLAEIKKS
                     ANQEGGGND"
     gene            6210..6620
                     /locus_tag="ACFNMK_001585"
     CDS             6210..6620
                     /locus_tag="ACFNMK_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HFC6364815.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLTAEMAALKKPDALIHGKEVRQKERNSSQG"
     gene            6625..7173
                     /locus_tag="ACFNMK_001586"
                     /pseudo
     CDS             6625..7173
                     /locus_tag="ACFNMK_001586"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7189..7374
                     /locus_tag="ACFNMK_001587"
                     /pseudo
     CDS             7189..7374
                     /locus_tag="ACFNMK_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(7658..8020)
                     /gene="ssrA"
                     /locus_tag="ACFNMK_001588"
     tmRNA           complement(7658..8020)
                     /gene="ssrA"
                     /locus_tag="ACFNMK_001588"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8083..8688)
                     /locus_tag="ACFNMK_001589"
     CDS             complement(8083..8688)
                     /locus_tag="ACFNMK_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC6364816.1"
                     /translation="MNTDFQNILKQLGKQAKKEAAEKQEAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPSDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2247 a         1961 c         2312 g         2457 t
ORIGIN      
        1 ctgattaaag tgagtgcgca aagccatctg aaggcgatgt gtttgaacct gttgaaagcg
       61 gccaacaggc taagtgtgcc cgctgccgcc taaaaggcag cccggatgcc tgattatcgg
      121 gtatccgggc aggattaagg gggtgtttgg gtaaaattag gcggtatttg gggcgaaaac
      181 agccgaaaac ctgtgttggg atttcggttg tcgtgaggga aaggaatttt gcaaaggtct
      241 cagagtgagt ttattttggg gcggcggcag gtctggggca agcggtgtgg ggcttggttg
      301 tggtttttag gtttttgggg gtaaaaaatg ccgtctgaac ttttcagacg gcgtttgttt
      361 tttctatcca atcgaggaac tgccgccatt tttccagcgg catatcggcc cgacgggttt
      421 cggtatcggg ttcggcttcc cagcggccta cctgtatgta gtgcttcacc ccgacgattt
      481 gcgccaactc ggcctgtgtc agtttgcagc ggttgcgaag ggtgcggagg ttgtaaggcg
      541 tgtagccgag ttccatgtcg ttgcggttta ttttgtttcg catatttttt tgactgccct
      601 gcggcaggtt tcggtaagga tggcggcaaa tcgggcttcg tctgccggtt tgccgtcgaa
      661 aaaaataaaa tcgtaaaggg tgatgccgtt ttcggaatgg cggtaatgcc tgcccgggca
      721 ttcgccgtct gtctgcctgt ggattttggc aatcaggcgg ggatagcggc aatgggtaat
      781 gaaacggctt gcgccgtctt gcccgataat ccattcgggg tagcggttga atagcgcggc
      841 tctgttcatt ttgttcgtgg gataaagccc cttgcggggc ttgtggtcag gcaaatttga
      901 atatcagtgc caacacggcg gcgatggcgg taaccagccc ggttgccgct atcatgggat
      961 accagcggga ctcttgggct atttttacgg attcggcgtt gattttgtgc gcgtcggcaa
     1021 tgattttggc gatttcggcg tctatttttc ttaactcggc gcgtttcgtt tcttgttcga
     1081 ttatgttcat tggatttcct ttcgtttttg gcggttgccg ccgcttgtcg gatggcagga
     1141 tgtctgccat gtgtgtacat taataccttt taggttttat tgcaagtgtt ttgggcagga
     1201 tgccgtctga atttttttca ggcggttttt tgttttgcgg ggttttggcg gcggcttcgt
     1261 atgcttggcg gtggcggcgg ctgtaccatt cggcgagttc gcgccgctct tggaggcggt
     1321 agctgtcggc ggtaggtagt ggtgcaggct tgagaagccg gcgcgttgga gggcggcgcg
     1381 gctgatgtgg ccgagggcga ggtctgttgt gagggtgtct tttccggcgg tcaggctgat
     1441 gttggtgaag aggtggcgga atccgtgcat tgtgtgtttg gatttgccgg gggtgctgcc
     1501 gtcatagccc agtcgtcgga tggcgttgtg ggcgaatttg atgctgatgt ggtcgggatg
     1561 ggggggcggg tttgcgccgt gggcggatgc cggggaaaag gtggatgttg tcgccggtct
     1621 gtgtgtgcag ctctcggagt atttctaccg cccagtccga cagtgggacg gtaaaggggt
     1681 gtttggtctt catgtcggcg gcggggatgt gccataaccg ggcggtgagg tcgatgtctt
     1741 gccagcgggc ggaaagcagt gcggagggac gggatgactt ccccggtttc cccatcaatc
     1801 cgcgccgcct cgatttttcc gttggcgcgc agcatttcca ccgcctgccg caccgacatc
     1861 aaccgcttgc cgttgcgcct catcgcctcc accaaaaccg ccgaaatcaa tttggcttct
     1921 tccggtttaa gctccgtctt gcccgcatcg ctgcgccgtt tgcgcgtcgg cttgacgctg
     1981 accgcctcca gcttgcggta tagcgtggca aggctgatgc ccaattcctg cgcctgctgc
     2041 ttaagatatg cagagcgtgc gccgcgtccc attgcttccg cctgattctc gactgcctta
     2101 agacgctcaa tcattgccgg attcatcgcc ttctcccgtt tcaccgccca accattccgg
     2161 cacattgtct gtcggtgctt cagtcggcag ggcatagctt tcgcgcagtt gctcgcagtc
     2221 caaaataatt tgattgagcg tgccgaccat ctttgcctga tggctgatct cgtgtgcctc
     2281 actgtgcgca ttaagttggt cgaacaaatc tttcaaacgg ctcacttgac tgcggatacc
     2341 gacctcaagg cttgttaact gcatcattta tgatttgtta ggggttttag actgctatca
     2401 aaaatcacag tcctgtccca accctaaaag ccactgttgc tttgtttcag acgacatttt
     2461 tctatggtat ggatcaagac caacgtttga aatcagtctt gatctaactg gaaagaaaaa
     2521 agaaacatcc tctgcaaaga aatttgtgat gccttgtatt aatttccgtc accataggat
     2581 tgaaaaagaa acctacttag gaaattggaa tgaacaaatt gccgcattgg cagtaaaaca
     2641 aaatatagat tggtgtccaa gttttgatat tgagaatttt agacagtatg aataattact
     2701 atctatatag gaattgcagc agcgatgtgt tatgggtcaa acgtatccaa cgccaaatcg
     2761 acggcagcct actcttgatt tctgacaatt caacctatcc acccatgccc ttggcactgg
     2821 cggaacaccc cgatattcaa atcatcgggc aggtagtgca ggtatcaaaa gacttgaact
     2881 agacacaatc aaaaagggaa atagaatgaa aatactcgct ttattaattg ccgctacctg
     2941 tgctttatct gcgtgtggcg gccaagctga agaacaaccg gcatctgccc aacctcaaga
     3001 gcaggcacaa tccgaattaa aaaccatgcc ggtaagctat accgactatc aatcagtagc
     3061 caataaaggg cttgccgacc aaaaaactgg tctgaccctt ccagaacatg ttgttcctgc
     3121 cgacaatgcg gaagggaaga atctgctgca tgacttttca gacggcctca cattaaccgt
     3181 tgataccgat aaagccgaca aaattactgc cgtccgagta gtttggaata cagatgcagt
     3241 gcctcaaaaa gcggaaaaac tgtccaaagc cgccgcagcc ttgattgcgg caaccgcccc
     3301 aggagaccgc acactgctgc gtgataccgg cgaccaaatc aaaatggcaa ttgacagcca
     3361 taatgcgcaa aaagagccaa cccgagaatg ggcgcgtggt gggattgctt ataaagtcac
     3421 tgttactaat ttgccgagcg tggttttgac agcaaaagct gaataacctg cattacagcc
     3481 acaattagga gcagacggag atgatgttta tagagcctgc tgagaataaa taactgataa
     3541 aaaagctgtc tgaaaaggtt tcggatagtt aggatagcta tctattttta atatattaga
     3601 gagaatatga aaaataaact gactttacgt tatttcaaaa taactcgttg cggttattgg
     3661 tctggttctt caaaagaacc taatcattat gaggacttat taaaaacttt aactcattta
     3721 gagacatatg ttaaaggtaa aactattgaa gagactggaa ttagtcgtga tgataaagag
     3781 ccaagcactt tcttgctcga tatctgcaaa aaaaatggtc tgtggttgct tgctttttgg
     3841 aatcctgccg cagaaagctc ttcagatgaa attgtttctt taaacatgaa aagtaaacta
     3901 ggtcaagttg attctagccc agtaacagca ggaaatggga aaacttttgg cttcgttagt
     3961 tattttctta tttggccaga acataatttt tactcaacga ttctttctcc taatgcaact
     4021 ttagctggac gaacagagtt tgaagagtac atgcttaatt tcttgtggat tgatcctaat
     4081 tttatagaaa agactaccta cgaagaaact tcaacagacg gaataattca taccatggag
     4141 cgaactaatt ttaaattgcc tcttaaaatt aatggaactc ctgtgccacg ttttgcgggg
     4201 aaactagcta aatctccttc agaaaaagag tatgtcatta gtaattatga ccaagtaacc
     4261 caagtgcata atcaatgcac ggtaaaggtt acctctggac aaagttcaag tggaattttg
     4321 aagaaaatgt taccaagctt ttttggttca acagaattgt ttgctccatt aaaaaccatt
     4381 cgtgtaaatc attctgtttc tgctgcattt gaaaacaaat caaatcttaa ggattggatt
     4441 gaagactggg aaaaaaattc ttttgattta gataaagatg attgtggctt taagttaaaa
     4501 aacgatgaca agatttattg gattcgagga aatgtcatca aatgtgaatt agacgtcaac
     4561 cttcataagg aaaatgggat ttatccagct gctaaaattc tagatataat agaccaaagg
     4621 aaagattacg ttttaaacga attcaaatta agtgatgatt cttagaaata ttttcctaat
     4681 atgctctgca ttcatgattg gtgtagcctg gtatttaggt gctaatatcc gatttgaaga
     4741 gcaatgggta ttgtatgaag ccttgagaac aactgcatca attatttttg cagttgttgg
     4801 ggtttggttg gctattgtct atcccgaacg attaaaaagc ccttttagtc aaactttctc
     4861 ctctccggta tatcggcgtg gattcaaaca gcttttttcc cctgttataa gttccatttt
     4921 tattttaagt attgtgttac ttgttggttt gttggctcct ttgctcaaac atattgaggc
     4981 aataattcaa tatatcccac tttgtcggaa aatctcattt agcctgttag caagcttgac
     5041 gatgtggcaa atttatacgg ttttatctgt attagtgcct ttgttaaata ttttaagtaa
     5101 aaatgaaaat gactgtcatg catccaacat tattaacaag ctaaagggtg aacagaatta
     5161 gctatgtgat ttttagtgta aactgctttt aactcgtatt aaaaggccac tcttttaata
     5221 cctcctaaaa tccctgtacc tatcaataga tacagggatt ttttatgtcc accaaattca
     5281 accaattcat caaccgcgtc ctctcccacg agggcggtta cgtcaatcat cccaaagacc
     5341 ccggcggcga aaccaattgg ggcatcacta agcgcaccgc acaggcaaac ggctacaacg
     5401 gctccatgcg cgccatgacg cgtgaacagg ctatcggcat ttaccgaaag gcgttttggg
     5461 agcgttaccg cgccgaccaa atgccggaag cggtcgcgtt ccaatttttt gatgcctgcg
     5521 tcaaccacgg ttacggcaat gccgtccgta tgctgcaacg cgccgcaggc gtggcggacg
     5581 atggaatcat cggcgaaatc agcctcaaag ccatcaattc cctccccgaa aacgaccttt
     5641 tattgcggtt caacgccgag cgtctggtct tttataccaa gctcagtacg ttcacctctt
     5701 tcggcaaggg ctgggtacgc cgtgtggcgc aaaacctgat tcacgcgtct gcagataaca
     5761 ctgattaaag ggggagacaa accatgtcaa aaaagtcact catcgcccta atgaccgcag
     5821 ccatgcagcc cgatttcagc cacagcgacc taggcattcg ctacgccatg ccgactcagg
     5881 gatgttggac gcaagcccac cgcaagagcg gggtagccgc cgcgaaacgc gcagccaaaa
     5941 aaaagcgtcg caaataactg cctttttccg atggctgggc ggcttggtct ctaatccggc
     6001 cacaggaaaa atcagccata ccaaactatg ggcaaacgtc gccgcagccg ccatgacttg
     6061 gaagttcgtg caggcggcgg acgcccccga atggctttgg tgggcatacg gcgcgatggt
     6121 cggcgggtat gcattaatca aacgcggcat cgccgccgta ccgcagttgg cagaaatcaa
     6181 aaaatccgca aatcaggaag ggggcggcaa tgattgaatt tgtccgagcc aaaaaacggc
     6241 tgctttgggc atttgtgctt ttgcttgtgt ggacgtgcgg ttaccgatac gccgccgaca
     6301 aggccgaagc ggaacaaacc gccctgattg ccacctatcg gcattcttct atggttgcgg
     6361 cggaacaata cgccttgcag cttaaaaaag cgcaggacga aaggcagcgg tggtacgact
     6421 tttcccaaaa acaaggaaga aagcccgtga aaaaacagta tccgccgcaa acgaaaaaag
     6481 ccggctatct gaaaaccaag gaagaactgc ttgcggaatt ggcttgcctt acagcggaaa
     6541 tggctgccct aaaaaagccc gatgccttaa tccatgggaa agaagtgcgg cagaaagaac
     6601 gcaactcgtc gcagggttaa ggcaatgcca tccgttgaac tgctgttgga gattgtcgga
     6661 ctgccacgcg gcacctttta ttaccaattg gccgtccaat cggcagaaga caaatatgcc
     6721 gatttgaaac agcatatcca tgatatttat cgacgacata agggaagata cggctaccgg
     6781 aggattgcag cagccatcct tcatgcagga acaccggtca atcacaagaa agtcagccgt
     6841 ctgatggcga agacggggct gaaggcagtg atacggcggc gcaaataccg ctcgttcaaa
     6901 ggagaagtcg gcaaaattgc gccgaatatc ctgcgacgct gtttccatgc agaaaagccg
     6961 aatgagaaat gggtaacgga cgttaccgag ttcaatgtag gcggagaaaa gatatacctt
     7021 tctccgatta tggatttgtt taacggggaa atcgtcagtt accgtattca aatccgcccg
     7081 actttcgatt tggccggcga gatactgaaa ggtgcgccgg agaaaccggg atcgtctgaa
     7141 aagccgatac tgcattcgga tcaaggttgg caatatcaga tgttttttta tagtggatta
     7201 aatttaaatc aggacaaggc gatgaagccg cagacagtac aaatagtacg gcaaggcgag
     7261 gcaacgccgt actggtttaa atttaatcca ctatacaaca acgatagaat caagttgaaa
     7321 ttaaaagggc tgagccttgt tcagtacaga attcagtccc tgaaagccgc ttgattaaac
     7381 tgtccgactt tttggggtca gttcggcttc ggcatttttt tatccgtttg ggggtaactt
     7441 gtttggaaag ctgcaagctt ataaataaag gattacattt aagttttggg taaccttttt
     7501 aaaaaaatgc gtgatgactt ttgcattttt aaggcgtttt ttggggtaat tcgtgaaaag
     7561 ttaccccaaa agttacccca taaatggcga aaactcaagc atacgccagc atattcaagg
     7621 cttttttgtg ttgcaggatg ctgctgaaaa tagggtatgg tggaggcggg gggaatcgaa
     7681 cccccgtccg aaagtcctct acaaagcgtt ctacatactt agttgtgtct atttgaaaat
     7741 cttatttcca tcatgccgac caacaggcct tttggaaacc agttacctta aatctcattt
     7801 cctgccaagt aacccggtag gaaaccagtc aatgtaagat gacgttgcgg tggctttcgc
     7861 cacacagccc attgaccgac tgctgcaacg gctggcctta agcggccaaa gcgtaagttt
     7921 cgtcgtttgc gactatttga attcagtgtt ttacgggaat ctgagacccc ggtatgcccg
     7981 catctgcttc gcaacccccg tcgaaaccaa ggtcgccccc agaaatggtt tgcaaattat
     8041 acggatattg tgcggtgctg ccaagtctgt cggagaaatt tgtcagtctt gctgccttaa
     8101 tttgcgtttg agcaggatgc ggacgcagcc gtcgttgcct tcccggggtt cggcgtaggc
     8161 gagtacgtcg gggtgttgca tcagccagtt tcgggtcata tttttcagaa cgggtttgta
     8221 gcctttggaa cctaatccgc tgccgtggat gatttcgccg catacgccgc gtttttgggt
     8281 gaatgcgatg aattcgttga ggactttttg ggcttcttcc tgtgtgtagc cgtgcaggtc
     8341 gacatcggta acgacgggat agtatccgtt tttcaggcgt tggatgtcgt tttttccctg
     8401 tccgtttttg ctgaagctgg cgggcgggtc gttgtatgtg ctgccgatgt agaagtagtt
     8461 ttcttcgtcg gcgcggttgt ctttgggacg gactttgatg ggggttttgt cggacggtgc
     8521 ataatattgt tgccggtttt ttaacgggga gacttgtccg actgcttgtg aaaaatcgaa
     8581 atcctgttct tgtttttgct tgtttttttc ggcctcctgt ttttctgccg cttctttttt
     8641 ggcctgtttg cccagttgtt tgaggatgtt ttggaagtcg gtattcatat tttttcctgt
     8701 tatttgtccg atggctgttt cgggcggggt tttaatttgc cggaatgttt gccaagcggg
     8761 ggaggatgat tttgttgccg gcgtatgttt tttgaaagtg tgattgtata tcaaaaagaa
     8821 atgcggcaac cgtcggcagt gttgattgcc ggaaatgcgg gccggtcgaa ccgatatgcc
     8881 cgaacgcctg ataaagtttt aaaaacctgc cttgcgaagc aggttaatgg ttttgccaat
     8941 cttgaattgc cggaaacgcg aaacacggaa atctgat
//
LOCUS       DAXHTY010000078         8763 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXHTY010000078 DAXHTY010000000
VERSION     DAXHTY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.078"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(216..1433)
                     /locus_tag="ACFNMK_001590"
     CDS             complement(216..1433)
                     /locus_tag="ACFNMK_001590"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC6364817.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEAHRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1635..2363)
                     /gene="rph"
                     /locus_tag="ACFNMK_001591"
     CDS             complement(1635..2363)
                     /gene="rph"
                     /locus_tag="ACFNMK_001591"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC6364818.1"
                     /translation="MPDCIRISRAADSLRDIKITPHFLPHADGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2445..2909)
                     /locus_tag="ACFNMK_001592"
     CDS             complement(2445..2909)
                     /locus_tag="ACFNMK_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC6364819.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAIAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3126..3902
                     /locus_tag="ACFNMK_001593"
     CDS             3126..3902
                     /locus_tag="ACFNMK_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6364820.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVFSTENITPPQSGAAC
                     STDAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4040..4567
                     /locus_tag="ACFNMK_001594"
     CDS             4040..4567
                     /locus_tag="ACFNMK_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364821.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKICILRTSFI
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRAS
                     TDYIVSRNLKFRHLY"
     gene            4738..5592
                     /locus_tag="ACFNMK_001595"
     CDS             4738..5592
                     /locus_tag="ACFNMK_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC6364822.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNKHGIC
                     RFHDLFNPEQGAAYVVVNFISLLELAKEGLVRIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5633..6841
                     /gene="pncB"
                     /locus_tag="ACFNMK_001596"
     CDS             5633..6841
                     /gene="pncB"
                     /locus_tag="ACFNMK_001596"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC6364823.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEVELDALCQLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAVRLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6933..8651
                     /gene="argS"
                     /locus_tag="ACFNMK_001597"
     CDS             6933..8651
                     /gene="argS"
                     /locus_tag="ACFNMK_001597"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC6364824.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIADSPIVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQAVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGSIKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDANAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAESASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
BASE COUNT         2120 a         2405 c         2182 g         2056 t
ORIGIN      
        1 tctaaggtgc tgaagcaccg agtgaatcgg ttccgtacta tttgtactgt ctgcggcttc
       61 gtcgccttgt cctgattttt gttaatccac tataaattta atccactata ttttttgttc
      121 caaagtcaaa atatgccgtc cgaacattcg ggcggcagac aaaacggcac tgcccgataa
      181 aggcagtgcc gttgtccgtt tcaaaccgtg aaacatcagc ccaaattaaa ggctttatgc
      241 aataccctgg ttgccagttc catgtatttt tcatcaatca atacggaaac tttgatttcg
      301 gaggtggaaa tcatttggat gttgataccc tcttcggcga gcgtgcggaa gattttggcg
      361 gctacaccga cgtgcgaacg catacccaaa ccgactgcgg agactttgca tacggtgtcg
      421 tcgccatcaa tagaagccgc gccgatactg tcttggcgtt ccgacaggat ttccaaagtc
      481 tgcttgtaat cgccgcgcgg tacggtaaag gaaaaatcgg ttgtgccttc gctgccgaca
      541 ttttggataa tcatatcgac ttcaatgttg gcatcggcaa ccgcgcccaa aatctgatag
      601 gcaacgccgg gtttgtcggg tacgccgcgc acgttgatgc gggcttggtt tttatcgaat
      661 gcgataccgg ttacggcagc tctttccatg ttgtcgtcct cttcaaaggt aattaaggtg
      721 ccattgccgc cgtcttgcag gctgctcagt acgcgcaggc gcactttgta ttttccggcg
      781 aattctactg aacggatttg caaaactttc gaaccgaggc ttgccagttc gatcatttct
      841 tcaaatgtaa ccgtatccat gcggtgcgct tcgggtacga cgcgggggtc ggttgtgtaa
      901 acgccgtcta cgtcggtata gatttggcat tcgtctgctt tgagggctgc ggcgagcgca
      961 acggcggaag tgtcggaacc gccgcgtccg agcgtggaaa tattaccttc actgctgatg
     1021 ccttggaagc cggcaacgat gacgactttg ccggcggtaa ggtcggcacg cattttttcg
     1081 tcatcaatgc tttcgatgcg ggctttggtg tgggcggtat cggttttgag ggcgacctgc
     1141 cagcctgtgt agcttttggc atccacgccg atgtctttca atgccatcgc caaaaggccg
     1201 atggttactt gttcgccggt ggacaaaacc acgtccaatt cgcgcggatc ggggtgttcc
     1261 tgcatttcgt gcgccaacgc gaccaggcgg ttggtttcac cgctcatggc ggatacgacg
     1321 acgacaatgt cgtgtccttc ggcgcgggct ttggcgacac gtttggccac gtttttaatg
     1381 cgttcgggcg agcctacgga tgtgccgccg tatttgtgta cgattaacgc catgtttcgt
     1441 gctttcttgt gtgggattgg tcgggcagca tggtttgctg gaaaagcggc tattattact
     1501 attttttaca tgaagttcaa gaacggactg cgctttcccg cctgccgttt gacagcggtc
     1561 agcgaaaaac ctgttctttc agattgttga caaaatgccg tctgaacggt tttcagacgg
     1621 catccggacg acaatcaggc ggcggacaac gcattttgct ggtgttgcag cagttcgcct
     1681 atgccttttt gcgccagtgc aaccagtttg cccaattcgt ccaaactgaa cggcgcgtct
     1741 tccgccgtcc cctgtatttc gatgattttt cccgatgcgg tcatgacgat attcacatca
     1801 ctgtcgcaac cggagtcttc gggataatcc aaatccaaaa gcggcacgcc gttcactacg
     1861 cctactgaca cagcggcaac ggcttcgcgg atggggtttt cactcaaaat gccgtctgaa
     1921 accagtttgc cgacggcgat ttgcagcgcg acaaacgcac cggtaatcga agccgtgcgc
     1981 gtaccgccgt ctgcctgaat cacatcgcag tcaatcaaga tttgtcgttc gccgagtttt
     2041 tccatatcca cgaccgcgcg cagcgaacgc ccgataaggc gttggatttc ttgtgtgcgc
     2101 ccggactgtt tgcccgccga agcttcgcgg cgcatccggg aagcagttga ggcaggcagc
     2161 atcccgtatt ccgccgttac ccagccttgg tttttaccgc gcagaaacgg cgggacgttt
     2221 tcatctatgg aagcggtaca aatcactttg gtattgccgc attcaataag gcacgaaccg
     2281 tcggcgtgcg gcaggaaatg aggggtgatt ttgatatcgc gcaggctgtc ggcggcgcgc
     2341 gagatgcgga tgcaatcagg catactgccc tcccgttaaa aacagataaa ttaaaaagcc
     2401 ttaaatatga aaaatcacat ttaaggcctt caaactgaaa atttctacgc ctcttcggct
     2461 ttgctgcgga taatcaaaag cggcaggtgg ctttggcgca ttaccgtttc ggcaaaactg
     2521 cccattaaaa ggtgcatcag cccggtacgt ccgtgcgtgc ccaacaccag caggtcggca
     2581 ccgttttcat cggcataatc aaccaaatcc tgcgccattt cgcgcgcacc cttattggca
     2641 accagcaggt gtttgacggt attttccaca cccagttccc gggcggtgcg ctcggcggca
     2701 tccaaaacct cgttaccttg tgcaatggcg gctgcctcgt aactctcgtg ttgcaaaaat
     2761 tcgggggcga gcgccatata ttcggcagga ttggcaacgt gtaccaaagt cagacgcgca
     2821 ccgttgaccc cggcaagctc ggcggcatgt ttcagggcat tgatggacgt ttcactgccg
     2881 tcaacggcaa caaccagatg tttgtacata tcgtattctc cttttgcacc gcctcgcggt
     2941 gccctcttgt cggatgggcg cagggacagt ttgcgccgtt tcattataga cccgccgtcg
     3001 ggctttatac aacagccgaa cagcccgacc gctttccagt ataatatgcc gcttccgtgc
     3061 agtcaggcat tttttgccgg ctttcgttca ctttttgatt tgacgcaatc ttgcaggatt
     3121 cgaccatgtc cgacaacgct ttgacctctt cgcgacgctt cggcggcatc gccagactct
     3181 acggagactc cgccttggcg cacttttcac aggcacacgt ctgcgtagtc ggcgtgggcg
     3241 gtgtcggctc gtgggcggtc gaggctttgg cgcggacggg catcggacgt ttgactttga
     3301 ttgatttgga caacgttgcc gaatccaatg tcaaccgcca gctgcacgcc ctgaccggag
     3361 acttcggcaa agcaaaagtt accgccttgc gcgaacgcgt cgcccaaatc aacccagaat
     3421 gccaagtgtt tgaaattgaa gatttcgtta ccgaagacaa tttgccggaa tacttcggaa
     3481 aaggttttga tttcgtcatc gacgcgatcg accaagtgcg cgtcaaagca gcaatggcgg
     3541 cttattttgt ggaacgcaaa caaccgtttg tcctcagcgg cggcgcgggc ggacaaaaaa
     3601 atccggcgtt aatccaaacc gccgatttga gccgcgtaac ccacgacccg ctgcttgcca
     3661 acctgcgcta caccttgcgg aaacgctacg gattcagccg cgatacgaaa gcaaatatgc
     3721 gcgtgccgtg cgtgttctca accgaaaaca tcacgccgcc gcaatcgggc gcggcgtgtt
     3781 ctaccgatgc cgcaccgcag ggcttgtcgt gcgccggcta cggtgcaagc atgctcgtta
     3841 ccgcttcgtt cgggctatat tgcgcacagg cggcggtgga acacatcgca gacaaaaaat
     3901 aagcaatgcc gtctgaaaca ggattcagac ggcatttgaa caaactatgg ttatgattta
     3961 agacaacaaa ggatacggat aaaaaataac ataaaatata tgattcctaa taatatacca
     4021 agtatcggag agctatttaa tggaattcgt taataattta gttatttttt catttttatt
     4081 actaatgctt attccgatat tttttgtagt atatggtata taccataaga tacgttatcg
     4141 caaaatatgt atcctaagaa caagttttat attattagtg gtaatacttt gcagtatgta
     4201 ttacatatat tgccgttatc ttgaccaaca aaaagtagct tattattgca tagatgaaca
     4261 atgtatttct attgttcatc tatacaaaga ttatggtata aactctccca catatgcgag
     4321 aatttacgca ggaaaaatat tgtttagatt tcaagtaaga gctaaaaatt acgctgaatt
     4381 acttatggaa gatgatatat caattagtaa aaaaattttg gggaataaat ttatcattta
     4441 tgggtcgcta cctgtaatat acggtaatgt agataatatt gaagtaaaag aagctactgg
     4501 ttatatagat agagccagta ctgattatat tgtctcaaga aacttaaaat tcagacattt
     4561 atattaatta agaggtttta gcaagaccgt attcacaacc tgctcctttt caaaacattt
     4621 gcatttaaaa gccgttataa tgccgtctga acatctgccc gaccacatta tacgtgaatg
     4681 tcggcagatt gttttctttt gtaaacttat attaaaatcc acttaccgat tcacgccatg
     4741 ccgcccatcc ctgccccatc cgcaccatcc gagcacactg tcgcatgggt attcggccaa
     4801 cccgttaccg atttgccgca ggatttgttt atcccgcccg acgcgctgaa agtcgtattg
     4861 ggcagttttc aaggcccttt ggatctactg ctgtatctga tccgcaagca gaacatcgat
     4921 gttctcgata ttccgatggt gaagattacc gagcagtatc tgcactacat cgcccaaata
     4981 gaaacctatc agtttgattt ggcggcggaa tatcttttga tggcagcaat gctgattgaa
     5041 atcaaatcgc gcctgctgct gccgcgtacc gaaaccgtcg aagacgaaga agccgacccg
     5101 cgtgccgagt tggtgcgccg cctgctggct tacgaacaga tgaagctggc ggcgcagggt
     5161 ttggacgcgc tgccccgagc cggacgggat ttcgcgtggg cttacctgcc gctggaaatt
     5221 gccgtcgaag ccaagctgcc cgaagtctat attaccgact tgacgcaagc gtggctgggt
     5281 attttgtctc gggcaaaaca tacgcgcagc cacgaagtta tcaaagaaac catctccgtg
     5341 cgcgcgcaaa tgacggcaat cctgcgccgt ttgaacaaac acgggatatg caggtttcac
     5401 gacctgttca atcccgaaca gggcgcggct tacgtggtcg tcaacttcat ctcactgttg
     5461 gagcttgcca aagaaggatt ggtcagaatc gtgcaggaag tcggtttcgg agaaattcga
     5521 atcagcctca atcatgaggg ggcgcattca gacggcattt ccggcacacg gggcgggcgc
     5581 gatgtgttct aatacgcccc aagccgccac caaaaatcgg gagacacgcc atatgaccgg
     5641 catcatacat tcgctgcttg acaccgacct ctacaaattc actatgctgc aagtggttct
     5701 gcaccagttt ccgcagacgc acagccttta cgaattccgc tgccgcaacg cctcgaccgt
     5761 ctatccgctt gccgacatca aagaagactt ggaagtcgaa ctcgacgcgc tctgccaact
     5821 gcgctttacc catgacgaac tcggctacct ccgcagcctg cgtttcatca aaagcgactt
     5881 tgtcgattat ctcgaactct tccagctcca acgccgcttt gtcgaaatcg gcacagacga
     5941 taaagaccgt ctgaacatcc gcatcgaagg tccgatgata caggcgatgt tttttgaaat
     6001 cttcatcctc gccattgtca acgaacttta cttccgccgc ctggaaaccc ctgcagtcat
     6061 agaagaaggc gaacgccggc ttcaagccaa agccgtgcgc ctcaaagaaa tcgccgccgc
     6121 acaaaacccc gacgaaccgc ccttcctgat ttccgacttc ggcacgcgcc gccgctacaa
     6181 gctcgcgtgg caggaacacg tcatccgcac cctgctcgaa gccgccccaa gcatcgtgcg
     6241 cggcaccagc aatgtctttc tcgccaaaaa actcggcatc acccccatcg gcaccatggc
     6301 gcacgagttc ctgcaggcat tccaggccct cgacgtacgc ctgcggaatt tccaaaaggc
     6361 cgcgctcgaa agctgggtgc acgaataccg gggcgatttg ggcgttgccc tgaccgacgt
     6421 ggtcggtatg gatgccttcc tgcgcgattt cgacctctat ttcgccaaac ttttcgacgg
     6481 gctgcgccac gacagcggcg acccttacgt ttggggcgac aaagcctacg cccactatca
     6541 aaagctcaaa atcgacagcc gcaccaaaat gctgaccttc tccgacgggc tggacatcga
     6601 acgctcttgg gcattgcacc aatatttcaa agaccgcttc aaaaccggct tcggcatcgg
     6661 caccaacctc accaacgata tggggcatac gcccttgaat atcgtcttga aactggtcga
     6721 atgcaacggg cagtccgtcg ccaagctgtc cgactctccg ggcaaaacca tgaccaacaa
     6781 cagcaccttc ctcgcctacc tgcgccaagt gttcgacgta cccgaacccg aaacgccgta
     6841 aaccggcaga aaaagcgcac aattcctgtt tctgccgcat aaaatctttt aaaataccgc
     6901 ctgatttgaa tttaaccgaa agaccgaact tcatgaacct acatcaaacc gtcgaacacg
     6961 aagccgccgc cgcctttgcc gccgcaggca tcgccgacag ccctattgtt ttgcagccga
     7021 ccaaaaacgc cgaacacggc gatttccaaa tcaacggcgt gatgggtgcg gcgaaaaaag
     7081 ccaaacaaaa cccgcgcgag ttggcgcaaa aggtcgccga cgcattggca ggcaacgccg
     7141 tcatcgaaag cgcggaagtc gccggtcccg gttttatcaa cctgcgcctg cgccccgaat
     7201 ttctcgcgca aaacattcag acggccttga acgacgcacg tttcggcgtg gcaaaaaccg
     7261 acaaaccgca aaccgtcgtt atcgactatt cttcgcccaa tctggcgaag gaaatgcacg
     7321 tcggccacct gcgttccagc atcatcggcg acagcatttc gcgcgtgtta gaatttatgg
     7381 gcaataccgt tatccgtcaa aaccacgtcg gcgactgggg tacgcagttt ggcatgttgg
     7441 tcgcttattt ggtcgagcag caaaaagaca atgccgcgtt cgagttggcg gatttggagc
     7501 agttttaccg cgccgccaaa gtgcgctttg acgaagaccc tgcctttgcc gacaccgcac
     7561 gcgaatacgt tgtgaagctg caaggcggcg atgaaaccgt tttggcattg tggaaacagt
     7621 ttgtcgatat ttcgctctcg cacgcccaag ccgtttacga cacgctgggc ttgaagctgc
     7681 gccctgaaga cgtggcaggc gaatcgaaat acaacgacga tttgcaagcc gtggtcgatg
     7741 atttggttca aaaaggcttg gcggtcgaag acgacggcgc gaaagtcgtg ttcttggacg
     7801 agtttaaaaa caaagaaggc gaacccgccg catttatcgt gcaaaaacaa ggcggcggct
     7861 tcctttatgc ttccaccgat ttggcgtgtc tgcgctaccg tgtcggcacg cttcatgccg
     7921 accgcctgct gtacgtcgtc gaccatcgcc aagccctgca ctttgagcaa cttttcacca
     7981 cttcacgcaa agccggctat ctgccggaaa acgtcggcgc ggcatttgtc ggcttcggca
     8041 cgatgatggg caaagacggc aaaccgttca aaacgcgcag cggcgacact gtaaaactgg
     8101 ttgacctgct gaccgaagcc gtcgagcgcg ccaccgcttt ggtgaaagaa aaaaatcccg
     8161 aattgggcgc ggatgaagcc gctaaaatcg gcaaaaccgt cggcatcggc tcaatcaaat
     8221 acgccgattt gagcaaaaac cgcaccagcg attatgtgtt cgactgggac gccatgctct
     8281 cgtttgaagg caacaccgcc ccctatctgc aatacgccta cacccgcgtg caaagcgtgt
     8341 tccgcaaagc aggcgaatgg gatgcaaatg cgccaaccgt tttgaccgaa ccgctggaaa
     8401 aacaactggc cgctgagctg ctgaaatttg aaaacgtgct gcaaagcgtg gcggacacgg
     8461 cgtatccgca ctacctcgcc gcctacctct atcaaattgc gaccctgttc agccgcttct
     8521 acgaagcctg tccgatactc aaagccgaaa gcgcaagccg caacagccgc ctgcaactgg
     8581 cgaagctgac cggcgacacg ctgaaacaag gcttggattt gctgggcatc gatgtgttgg
     8641 acgtaatgta aaaccgcacc gcccgattgc ggacaacagc cccaccatcc ttatccgaat
     8701 ctgaaaaaag cggcgcgata caccgtatcc gccgcccctc ccaaaatgcg aaacaaacaa
     8761 acg
//
LOCUS       DAXHTY010000079         8715 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXHTY010000079 DAXHTY010000000
VERSION     DAXHTY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.079"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..212)
                     /locus_tag="ACFNMK_001598"
     CDS             complement(<1..212)
                     /locus_tag="ACFNMK_001598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364825.1"
                     /translation="MPSESPSFRRHIHKGALVRGGGEGIVGGGAAFGENKKTLSDSDM
                     TNFPKKIPLKALTVFPCGRIVRFFAAA"
     gene            298..1482
                     /gene="coaBC"
                     /locus_tag="ACFNMK_001599"
     CDS             298..1482
                     /gene="coaBC"
                     /locus_tag="ACFNMK_001599"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC6364826.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGSNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNISSGKMGVALAR
                     ACRAAGAEVSLIYGQLQTELPFGISNRVQTVSAEDMHRAVHRLIDKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1549..3705)
                     /locus_tag="ACFNMK_001600"
     CDS             complement(1549..3705)
                     /locus_tag="ACFNMK_001600"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6364827.1"
                     /translation="MPASQPSAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3797..4003)
                     /gene="rpoZ"
                     /locus_tag="ACFNMK_001601"
     CDS             complement(3797..4003)
                     /gene="rpoZ"
                     /locus_tag="ACFNMK_001601"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC6364828.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(4063..4680)
                     /gene="gmk"
                     /locus_tag="ACFNMK_001602"
     CDS             complement(4063..4680)
                     /gene="gmk"
                     /locus_tag="ACFNMK_001602"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC6364829.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4808..5407
                     /locus_tag="ACFNMK_001603"
     CDS             4808..5407
                     /locus_tag="ACFNMK_001603"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC6364830.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5453..6241)
                     /locus_tag="ACFNMK_001604"
     CDS             complement(5453..6241)
                     /locus_tag="ACFNMK_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC6364831.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDSL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEQEGLKTVRWHEEAVDLLPAGASKMDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            6566..7921
                     /gene="yegQ"
                     /locus_tag="ACFNMK_001605"
     CDS             6566..7921
                     /gene="yegQ"
                     /locus_tag="ACFNMK_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC6364832.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLNVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDKNGWATIEVKNR
                     FAVGDSLEIIHPGGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            8055..8531
                     /locus_tag="ACFNMK_001606"
     CDS             8055..8531
                     /locus_tag="ACFNMK_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC6364833.1"
                     /translation="MLAVCQSGSAPKNAHTASAGTPAGESCKVIRDDTAGSGKNIIYR
                     CSNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
BASE COUNT         2208 a         2389 c         2057 g         2061 t
ORIGIN      
        1 gcagcagcga agaaccgaac tatacgccca cagggaaaaa cggtcaatgc tttcagtggg
       61 atttttttgg ggaaattcgt catgtcgctg tcggataagg tttttttatt ttcgccaaac
      121 gctgcaccac ctccaacaat cccttccccc cctcctctga ctagtgcgcc tttgtgaata
      181 tgccgtctga aactcgggga ctcagacggc attttgtatc caaaacggta actaatgtat
      241 ccgtactttg ttatagaatg gctgctgttt ttttcttcgt aattagaaat tgtcaaaatg
      301 ggtaaacata ttcttttagg tgtaacgggc agtattgcgg cgtataagtc ttgcgagttg
      361 gtgcgactgc tgaaaaaaca ggggcattcg gttacggtgg ttatgagccg ctcggcaact
      421 gaatttgttt ctccgctgac ttttcaggct ttgagcggca atcccgttct gaccgacacg
      481 cacggcagca acggttcaaa cggcatggaa catatcaacc tgacccggaa tgcggatgtt
      541 tttctgattg cgccggcaag tatgaatacc gtggcaaaaa tctgtaacgg cgtggcagat
      601 aatctactga ccaatctggc agccgcacgg aaatgtcctc ttgccatcgc tcccgcgatg
      661 aatgtggaaa tgtggctcaa ccctgccaac caacggaata tcgcacaact ggtttcagac
      721 ggcattactg tctatatgcc gggcttgggc gaacaggctt gcggagaaaa tggtatggga
      781 aggatgccgg aacctgccga attgctggat ttgcttccag acttgtggac accgaaaatt
      841 ttaagggaca agaaaatctt gattaccgca ggtgcgacat ttgaagccat tgaccctgtc
      901 aggggcatca caaatatttc cagcgggaaa atgggcgtag ctttggcgcg ggcgtgtcgt
      961 gccgccggtg cggaagtcag cctgatttac gggcaacttc aaaccgaact gcctttcggt
     1021 atatccaata gggttcaaac cgtcagtgct gaagatatgc atcgtgcagt gcatcgttta
     1081 atcgacaaac aagatgcttt tatttctgtt gccgccgtct cagactataa ggttaaaaac
     1141 aggagtacgc aaaaattcaa aaaagataaa aatgccaaac cattatccat cgaattggat
     1201 gagaaccccg atattttggc ttctattgcc tcattgccga acccgccgtt ctgcatcggt
     1261 tttgccgctg aaacggagaa tgtaatggca tatgcgcgag aaaaacgtat taagaaaaaa
     1321 ataccggtga tagttgccaa tgatgtttca atcgcgatgg gcaaaccgac caaccggatt
     1381 accattatcg gggacgacgg ggaactatct tttcccgaaa caagtaaaga tgaagcggcg
     1441 atgcagattg ttgaaaggct tgccgtatat ttgaacaaat aagcaattga acggataaac
     1501 cataaaacgg gttgcctgtt aatcaaaagg caacccgttt tacctgcttc aacttctgat
     1561 gactttgcgg atatatggaa tactatgcag attttgaata atctgattca actgatccaa
     1621 attcttgact ttcaataaga atttgaattc gacaaaacct tccgttcccg actgggattt
     1681 agacggtgtt tcgaccgact cgatgtccgc accggaatcg gaaatcgctt gcgccattaa
     1741 tgccaacagg ccgtggctgt cttccgattg gacttgaagc ccgacacggt agttctgccc
     1801 attcatattt tcccagtctg catccagctg ctgttcggga tcggacttca acaacgtcgg
     1861 gcagctatcc ctatggataa tcatgccttt tcccttaacc aacagcaaac ggatggaatc
     1921 gccgggaaca gggtggcagc actctgcaaa atgaatatgc ccgctttcct gaccatcgac
     1981 tttaatggaa ctgagcctga cctcgctgcc gaaatgctcc cctgccaact cagcaatgtg
     2041 catggcgaca taaacaggca gggtatgccc catccctacg ttgtacagca cttcttcaaa
     2101 cgatgtctgc ttgtcgttga gatcggcaag atatttttcc ttgatgccgt ctgaaagcag
     2161 gacatctttg ggcagcaaac tggacagggc tttttgtaag aggctctctc ccaaaacgac
     2221 cgcatcgtgc cggttaaggt ttttaatata ttggcgtatg gcactgcgcg ccctgcctga
     2281 cacggcgaaa ttcaaccacg cgggattggg tttggcgtgt tcggatgtga taatttcaac
     2341 agaatcaccg gttttgagct tcgtacgcaa cggcatcatg atattgttga tacgtgcggc
     2401 aacggttttg tgcccgatat cggtatgcac cgcataagca aaatcgacag gcgttgcccc
     2461 tttgggcaaa gttaggattt ttccttttgg cgtaaggatg tagatttcgt tcggaaacaa
     2521 atcgactttg acgtgttcga gaaactcaat ggcattggca ctgcttgcct gcaaatctaa
     2581 gatatttttc agccaccggt ttgtgtgaag caccgcctga tcgaccgtct tagaatatga
     2641 tttatagctc caatgtccgg cgattccacc ttcggcaaca gcatccattt ccttggtacg
     2701 tatctgaact tcaatcggca agccgtaagg accgaccaaa gtcgtatgca gactttgata
     2761 cccgttgctt ttcggaatgg cgatatagtc tttgaaccgc ccgggcttgg gctgatagag
     2821 ggtgtgcaat gcgccgagtg cggcataaca ggctggaatg ctgttgacaa tgacgcggaa
     2881 accgtaaata tccataacct cggcaaagcg cagctttttc gccatcattt tctgatggat
     2941 gccgtacagg tttttttccc tgcctttgat tttggcctct atattcgcgc ctaccagccg
     3001 ctggccgaat gcgcgcaaga ctttgccgac aacgtcctgc cggttcttcc ggctcttgtc
     3061 catcgctttt tttaaagtct cgtagcggtt gggatgcagg ttttggaacg ataaatcctg
     3121 aagctcttga tatgcgttat tcaaacctat acggttggca atctgtgcat agatttcaag
     3181 ggtttccctt gcaatccggc ggcgtttgtc cgggcgcatc gaaccgagcg tccgcatatt
     3241 atgcagacgg tcggcaagtt taacgacaat cacgcgcaca tctttggtca tcgccaaaat
     3301 cagtttgcgg aaactctccg cctgatgttc cgcatgatct tcaaatttga gtttttcgag
     3361 tttggacagc ccgtccacca tctcggcaac cgtattgccg aacaccgccg ccatttcccc
     3421 ttttgtcacg cccgtatctt ccaatacgtc gtgcatcacg cctgcacaaa gaccctgtat
     3481 gtccatatgc caaagggcga gctgcgtcgc aacggcaatc ggatgcgtga tgtagggctc
     3541 cccgcttttg cgggtttgcc cgtcgtgggc gcgaaacgca taggcgacag ctttttcaag
     3601 ctccgcctgt tcctcgggct tgaggtagga ggcggtatgg aaaagcaggg cacgcgcttc
     3661 ggcggtcagg gggtcgtaag gggcggaagg ttgggaggcg ggcatttcag acggctttcg
     3721 gtatgtatgt gtttttcatt tcaaaccgtc ggactgcacg gcggcagaat gtgccgacgt
     3781 gcgtttccgg cagaatttat ttattgcgcg tcaacagttc cgtaccgata tgcccagcgg
     3841 cgatttccct taaagcggta acagtcggtt tgttattgcg gacatcgtcc acaagcggcg
     3901 tattgccatt ttcaagctgg cgggcccgac gggccgctac caatgtcagg tcaaaatggt
     3961 tggaaatttt tccggtacag tcttcggtgg taatacgtgc catattattt gctttctttc
     4021 aaaaatattt aaattgggaa accgggtatt ttcgcggttt ttttaggaat tttccaacaa
     4081 atctgcaata aatcccagtt gccgcgacct tttcagacgg caggcattca caatatggcg
     4141 caaatcctcc tccgcccgcg ccaagtcatc attgaccacg acaaaatcga acaatacgga
     4201 ctgttcgatt tcatgccttg ccttcgacag cctcctttgg ataacttccc gactgtccgt
     4261 cccgcgtccg ttcagacgtg cagcaagcac atcgaaagac ggcggcagga taaagatgcc
     4321 gacggcttcc ggcagcgcgt tgcgaacctg cgccgcgccc tgaacgtcga tttccaaaat
     4381 tacgtcatag cctgccgccg ccaaagcatt cacgccctcc gcgcttgtgc cgtaatagtt
     4441 gccgaacacg tctgcgtatt ccaaaaaagc ttcctgcgcg ataagcgact caaactcttc
     4501 tttggaaaca aaatgataat gtacgccgtt tacttcgcct tcacgcggcg gacgcgtcgt
     4561 gtgcgatacg gaaacgcgca aaccgttatg gtttgccaac agccgcgaca ccagcgtggt
     4621 tttgcccgtg ccggaagcgg ccgaaatgat aaagatgttg ccttttcgat aagcggacat
     4681 attttttacc tgtatatttt ccagccgatt gtatcacaac ggacacccgg tttcctattt
     4741 gccgatgccc atattttgcc gctattgttt tgattgattt ggcaagcggc aggctgacgg
     4801 ctacaatatg gcgttaaaaa catcaaactt ggaacacgca atgctggttc atcccgaagc
     4861 tatgagtgtc ggcgcgcttg ccgacaaaat ccgcaaaatc gaaaactggc cgcaaaaagg
     4921 catcttattc cacgacatca cgcccgtcct gcaaagcgcg gaatacttcc gacttttggt
     4981 tgatttattg gtttaccgct atatggatca gaaaatcgac atcgttgccg gtttggacgc
     5041 gcgcggcttc attatcggcg cggcactcgc ctaccatctc aacgtcggtt ttgtccccat
     5101 ccgcaaaaaa ggcaagctgc cttttgaaac cgtatcgcaa agctacgcgc tcgaatacgg
     5161 ggaagctgcg gtggaaatcc acaccgatgc cgtcaaaccc ggttcgcgcg tgctgctggt
     5221 cgatgatttg gttgccacgg gcggcacgat gcttgccgga ctggagctga tccgcaaact
     5281 cggcggggaa attgtcgaag ccgccgccat tttggaattt accgaccttc aaggcggcaa
     5341 gaatatccgt gcaagcggcg cgcccttatt taccctgctt caaaacgaag gctgtatgaa
     5401 gggctgaaaa ccgaccctgc cgtctgaaac cggcagggtt gttattatgc gttcaaatca
     5461 cgcccaaatc ttgcaagccc ctcaacacgc cgtcttcatc aacgccgggg caaacatatt
     5521 tcgccgcttc tttcgccgcc tgttccccgt tgcccattgc cacgccgaac ccgacttcag
     5581 acagcatttc cacatcgttc aaaccatcgc cgaacgccat cacgtccgcc atttccaaac
     5641 ccaatgcttc aaccacgctt ctgataccgt ccattttcga cgcacccgca ggcagcagat
     5701 cgaccgcttc ctcgtgccag cgcaccgttt tcaagccttc ctgttccaca atatccgacc
     5761 agagcggcat ttcgttttcc tccgcaaaca ccagcatctg atacaccggt ttgcttgaga
     5821 aataatcctt atcggcaaaa aaatcgctgg cgatatgctg caaggcgcgg cacacgcatt
     5881 ccgacagcgc ggacacggcg atcccctctc cgccgacaaa cgcataatcc atacccaagc
     5941 tatccaaatg cgcgcaaacc ctgcccatca aaccggcatc catcggtact tcgcgcacag
     6001 ttttaccgtg cagcagcgca aactgcccgt ttatcgttac cacggcatcc atacccgttt
     6061 ccgccatcat gtccctgacc ttttcgggta tcgtcgccaa agaccgcccc gttgccaacg
     6121 ccgtcaatat gcctttgccg cgcaaagccg ccaccgccgt tttcacggaa ggacgcaagg
     6181 tatccgtata tttgcggtac agcgtatcgt caatatcgaa aaacacgatt ttgggattca
     6241 tcatattctc tctcgcactc aaactgccgc attgtatccc aatcaggcaa atacttgata
     6301 aatccttata aatttcccgg caaaattgac cgcgaacacg aaaaggcgga taatccgccc
     6361 atcctcaaac ccttttcaga cggcatttgc agcaatgccg tctgaaacat ttttacaaag
     6421 cacacaaatc atgtttcaac acacaggacg acacataaag cgtcgcccta tatgttgccc
     6481 tgattcggaa agggttacac ccctcccaaa taaagtctga ttctaccgcc ctaaagggcg
     6541 gggtttcaac cgaaaaggaa acacgatgaa agcacccgaa ctcttattgc ccgccggcgg
     6601 attggaaaga atgcgcgccg cctacgatta cggcgcagat gccgtttacg ccggcagccc
     6661 gcgttactcc ctgcgcgccc gcaacaacga atttgccaaa ctcaacgtcc ttgagcaagg
     6721 cattaaagaa gcgcacgagc gcaacaaaaa attcttcctt accgtcaaca ccctgccgca
     6781 caactccaaa ctcaaaacct ttgtttccga tatggaaccc ttgattgcca tgaaacccga
     6841 cgcgctgatt atggcggatc cgggtttgat catgaccgtg cgcgaaaaat ggccggaaat
     6901 gccgatccac ctgtccgtac aagccaacac caccaactac tggggcgtga aattctggca
     6961 gaatatcggc gtcgaacgca tcatcctgtc gcgcgaattg agtatggaag aaatcgccga
     7021 aatccgccaa caatgccccg acatcgaact cgaagtcttc attcacggcg cattgtgcat
     7081 cgcctactcc ggccgttgcc tactctccgg ctacttcaac caccgcgacc ccaaccaagg
     7141 cacctgcacc aactcctgcc gttgggacta caaagtccac aacgccacgg aaagcgatgc
     7201 aggcgatgcc caacttctgc aaggttttaa ctttgaaaaa gcccaagaag aagccaacca
     7261 aaactttgaa ggcatcaatg gtcaaaaacg ccatccctac gccgacaaag ttttcctgat
     7321 tgaagagtcc aaccgcccgg gcgaaatgat gccgattatg gaagacgaac acggcaccta
     7381 catcatgaac tccaaagacc tgcgcggcat tgaagtggtt gagaaactcg ccaaaatcgg
     7441 cgtggacagc ctcaaagtcg aaggccgcac caagtcgctt tattatgttg cacgtgtcgc
     7501 ccagtcctac cgcaaagcga ttgatgatgc cgttgcaggc cgtccgtttg attacagcct
     7561 gttgagcgaa ctcgaaggcc ttgccaaccg cggctacacc agcggcttcc tcgaacgcca
     7621 ccaaacccaa gactatcaaa actacctcag cggccattcc accgccaaac aaagccaata
     7681 cgtcggacac gttaccgaaa tcgacaaaaa cggctgggcg accatcgaag ttaaaaaccg
     7741 cttcgccgtc ggcgattcac tcgaaatcat ccacccgggc ggcaaccaaa ccatcaaatt
     7801 ggaacaaatg acccgcaaag gccaacctgt cgatgttgcc ccgggcaacg gcattcaggt
     7861 caaaatcccc gatatgcagg gcaaagaaaa agccctcatc gcacgcgtat tgaaccccta
     7921 agccacgatg ccgtccgaac cccgttttca gacggcattt tgttgaatct tacggtacaa
     7981 tggcaatgat ttcccaccgc aagcaaggat atccgacatg aaaaaaaatc ctcatactct
     8041 ccgccgccgc cctgatgctg gcagtctgcc aatccggcag cgcacctaaa aacgcgcaca
     8101 cggcatccgc cggcacaccg gccggagaat catgcaaagt catccgggac gacaccgcag
     8161 gaagcggcaa aaacatcatt taccgttgca gcaacggtca ggcggcaata aatgcagcca
     8221 ttgcagacgg cgtattggaa tctgggattc ccgtcagctt cggcggcggg ggcgtaccgg
     8281 ttgcaaacgg caggaatctg gttacacggc aagcagcaaa cggcgttggc aaatccgact
     8341 ccgaggcctg cgaacacgcc ctaatcaatg ccgcccgcaa attccagcag accgccggca
     8401 aactcggcgg gcgcagtgtt accggcttcc acagctactt tgacaagcag ccgctgcaag
     8461 gcggacaata cgactgtcag gcaggttcgt tccacgtccg cgtggtcatg cgcgccaatg
     8521 tcgtccgtta acatatcggc aatcaaaaaa ttaggctgtg ttttagtaat ctgttgattt
     8581 caattatttg caaaggaaaa gacaattatt ttttcggtta ggaagagacc tatcctattg
     8641 aatacattta agttaaatac gtctatcaac actatattat agtggattaa caaaaatcag
     8701 gacaaggcga cgaag
//
LOCUS       DAXHTY010000080         8563 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXHTY010000080 DAXHTY010000000
VERSION     DAXHTY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.080"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(78..596)
                     /locus_tag="ACFNMK_001607"
     CDS             complement(78..596)
                     /locus_tag="ACFNMK_001607"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC6364834.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTKQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(616..2001)
                     /locus_tag="ACFNMK_001608"
     CDS             complement(616..2001)
                     /locus_tag="ACFNMK_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC6364835.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGCTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            2240..3781
                     /locus_tag="ACFNMK_001609"
     CDS             2240..3781
                     /locus_tag="ACFNMK_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6364836.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWGAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRTFVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRCAVSDKAVSPVMPSSPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAATVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFALGIFLLLDNGEGGK
                     QTETL"
     gene            3810..4409
                     /locus_tag="ACFNMK_001610"
     CDS             3810..4409
                     /locus_tag="ACFNMK_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC6364837.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVELIRRHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            4406..5011
                     /gene="nadD"
                     /locus_tag="ACFNMK_001611"
     CDS             4406..5011
                     /gene="nadD"
                     /locus_tag="ACFNMK_001611"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC6364838.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDAVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLASQGVSDGIPPAAARYIREHGLYEK"
     gene            5070..5456
                     /gene="rsfS"
                     /locus_tag="ACFNMK_001612"
     CDS             5070..5456
                     /gene="rsfS"
                     /locus_tag="ACFNMK_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC6364839.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            5510..5980
                     /gene="rlmH"
                     /locus_tag="ACFNMK_001613"
     CDS             5510..5980
                     /gene="rlmH"
                     /locus_tag="ACFNMK_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC6364840.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARIMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            6053..6319
                     /locus_tag="ACFNMK_001614"
     CDS             6053..6319
                     /locus_tag="ACFNMK_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC6364841.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            6476..7159
                     /locus_tag="ACFNMK_001615"
     CDS             6476..7159
                     /locus_tag="ACFNMK_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFC6364842.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            7283..7795
                     /gene="coaD"
                     /locus_tag="ACFNMK_001616"
     CDS             7283..7795
                     /gene="coaD"
                     /locus_tag="ACFNMK_001616"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC6364843.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETVKRYVPPAVYQ
                     KMIAEHQHEN"
     gene            7827..8480
                     /locus_tag="ACFNMK_001617"
     CDS             7827..8480
                     /locus_tag="ACFNMK_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFC6364844.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1866 a         2265 c         2502 g         1930 t
ORIGIN      
        1 caatgtaatc cattctgttc ccgatgttta tgccgtctga aacccatcct gtgtcgggct
       61 tcagacggca tattgcttca aagcaggttt tccgaggcaa cccagttcag aatatcggct
      121 tcgaccgctt cgggcgtgcc ggggttggcg attttgaccc cgtattcgcc ttcgcccagt
      181 tcctcgagga tttccagttg cgaatcgagc atccctgctt tcatgtaatg tcctttgcgc
      241 gacatcatgc gctcgaggtt gatgtcttgc ggcggactga ggtggatgaa ggcagctttg
      301 ccttcggctc cgcgcagaat gtcgcggtag ccgcgtttga gggcggaaca ggttacgatg
      361 gtgtggttcg caccgttttg cgcctgtttc gtcatccagt cgcgcagatt gcccaaccac
      421 ggatagcggt cttcatcggt cagcggaata cccgcgccca tcttgtcgcg gttggcttgg
      481 gtgtggaact cgtcgccttc ggcataggga cattgaccga ggtgtttctg cagggacagc
      541 gcggcggtgg tcttgccgca gccgcatacg cccataacga caaaatgcgt agtcatttac
      601 tatcctttcc gtctgtcaga cgatggcgaa cagcagtgcg gacaaggcaa agccgatgag
      661 tgcgatgagg gtttggttga ccgtccaggt tttcagcgtg gtcggtacgt ccatgtccaa
      721 gaggcggccg accagccaga agccggagtc gttgaagtgg ctgcaaccga ccgaacctgc
      781 cgccgttgcc aatacgatac aggcgagctg ccagtcggta aagccggcgg cggcaacggc
      841 aggagccatc agcgcggcgg cggtggtcag ggcgacggtt gccgaacctt gcgcgatacg
      901 cagtgccaag gcgacaagga aacagcccaa aaggacggga atgcccaaat ccgccatgct
      961 gtcggcgagt gccttgccga tgccggaagc gcgcaaaacg ccgccgaaca taccgcccgc
     1021 gccggtaatc agaatcacgg aacagacggg ggcgagtgcg ccgtccacgg ttttttccaa
     1081 cgcgctgccg ctttcgccgc gtttgcgtcc caagacaaac agtgcgacca atacggaaat
     1141 cagaagggcg atcggtgtcg aaccgattat ttttgccgtc tgaacccagg tttcgtccgc
     1201 acttacgagt ttttcgctga tgagggccga tacgccggta ttcaggaaaa tcagcagcat
     1261 gggaatcagc atgatggcga cgaccgttcc tgctttggca ggttctttcg gcaggtcgtt
     1321 gtcttgcgtg ccgccgctga gcagttcggg aacgggaaca tggatggtgc accccaacac
     1381 tttgccgagc atatagccgc tgaaatacca tgtgatgaag gcggtcggca gacccaaaat
     1441 caaaacttgg ccgatgttcg cgccgtaaaa ttcggaagcg gcaatcgggc ccggatgggg
     1501 cggcaggaag acgtgcatga cggaaaatgc gccgatggag gcaagcgcga agggcagtac
     1561 gtcctgtttc atgcgccgtg cggtggcgaa cacgatgggc agcatgacga ttagtccggc
     1621 atcgaagaaa atcgggaagc cgaaaatcag cgaggcaacg cccagcgcga acggtgcgcg
     1681 tttttcgccg aacatccgga tcagcgcgtc cgccagcgac tgtgcgccgc cggatgtttc
     1741 gaccagacgt ccgagcatcg cgcccaggcc gaccagaagc gccacgccgc cgagcgtgcc
     1801 gccgaagttt ttgaccagta cgtcgttgac aatgctgcct gtgggcaaac cggttgccaa
     1861 agccgtcagc aggctgacga tgaccagtgt cagcagcgcg tggatgcgga atttgacgat
     1921 taaaatcaga atgaggatga ttgccgccgc cgaaatgccc aacagggttt gcgcggacag
     1981 cgtctgtgtc cagccgtcca tttgtaaagc ctttctctgt aataaaagta caaaattgtc
     2041 aggttttttt aagttgtgcg aaaacgatag cacaaatcgg gcggtaagtt gtttgtctga
     2101 agttatattc ctgtttattt gaacgattta tttcagacgg cgggaggaag gcggatacgg
     2161 tatgtagtcg gattgaaata tacggtatgt ggggcttttg ggtacaatcg caacatcagt
     2221 gtttcaagtg gggaaggcga tggatggacg gcgttgggcg gtatggggtg cttttgccct
     2281 gctgccttcg gcttttttgg cggtaatggt cgttgcgcct ttgtgggcgg tggcggcgta
     2341 tgacggtttg gcgtggggcg cggtgctgtc ggatgcctat atgctcaaac gtttggcgtg
     2401 gacggtgttt caggcggcgg caacctgtgt gctggtgctg cctttgggcg tacctgtcgc
     2461 gtgggtgctg gcgcggctgg cgttcccggg gcggactttc gtgctgcgcc tgctgatgct
     2521 gccgtttgtg atgcccacgt tggtggcggg cgtgggcgtg ctggctctgt tcggggcgga
     2581 cggcctgttg tggcgcggac ggcaggatac gccgtatctg ttgttgtacg gcaatgtgtt
     2641 tttcaacctt cctgtgttgg tcagggcggc gtatcagggg tttgtgcaag tgcctgcggc
     2701 acggcttcag acggcacgga cgttgggcgc gggggcgtgg cggcggtttt gggacattga
     2761 aatgcccgtt ttgcgcccgt ggcttgccgg cggcgtgtgc cttgtctttc tgtattgttt
     2821 ttccgggttc gggctggcat tgctgctggg cggcagccgt tatgccacgg tcgaagtgga
     2881 aatttaccag ttggtcatgt tcgaactcga tatggcggtt gcttcggtgc tggtgtggct
     2941 ggtgttgggg gtaacggcgg cggcagggtt gctgtatgcg tggttcggca ggtgcgcggt
     3001 ttcggataag gcggtttccc ctgtgatgcc gtcgtcgccg cagtcggtcg gggaatatgt
     3061 gctgctggcg tttgcggcgg cggtgttgtc tgtgtgctgc ctgtttcctt tgttggcaat
     3121 tgttgtgaaa gcgtggtcgg ccggcgaatc gtggcgtgtg ttaatggaaa gtgaaacgtg
     3181 gcaggcggtg tggaatactt tgcgcttctc ggcggcgacg gtgtatgcgg cggcggtttt
     3241 gggtgtggtg tatgcggcgg cggcgcggcg gtcggcgtgg atgcgcgggc tgatgttttt
     3301 gccgtttatg gtgtcgccgg tttgtgtttc ggcgggcgtg ctgctgcttt atccgcagtg
     3361 gacggcttcg ttgccgctgc tgctggcgat gtatgcgctg ctggcgtatc cgtttgtggc
     3421 aaaagatgtt ttgtcggctt gggatgcact gccgccggat tacggcaggg cggcggcggg
     3481 tttgggtgca aacggctttc agacggcatg ccgcatcacg ttccccctct tgaaaccggc
     3541 gttgcggcgc ggtctgactt tggcggcggc aacctgcgtg ggcgaatttg cggcaacctt
     3601 gttcctgtcg cgtcccgagt ggcagacgct gacgactttg atttatgcct atttgggacg
     3661 cgcgggtgag gataattacg cgcgggcgat ggtgctgaca ttgctgttgg cggcgttcgc
     3721 gctgggcatt ttcctgctgt tggacaacgg cgaaggcgga aaacagacgg aaacgttata
     3781 atgtgaaccc tttttcagag gacggcaaaa tgagcgagtt ggataacatc cttgcccata
     3841 accggcagtt tgtcgagtcg ggcgaatatg aaaaatactt taccgacaaa taccccgaac
     3901 gcgggctggc agttttgtcc tgtatggatg cgcggattat cgggctgctg cccgacgcgt
     3961 tgggtttgaa aaacggcgat gccaagctga ttaaaaatgc cggcgcgctg gttacgcacc
     4021 cgtggggttc ggtgatgcgg agtcttttgg ttgccgtgtt tgaattgaag gtcagagaga
     4081 ttatggtcat cgcccatcac gattgcggta tgcaggggct gaatgccgaa gaattcctcg
     4141 ggcgcgtccg ggaaagccag attcccgaag accgtatcga aaccctgcgt tatgccggta
     4201 tcgacctcga cggctggctg accggtttcg acaacgtcga agacagcgtg cgccatactg
     4261 tcgaactcat ccgccgccat ccgctgatgc cgcgccatat tgccgttcac ggattggtca
     4321 tccatcccgt taccggcaaa ctgacgctgg ttgtggacgg cagtgtttca gacggcatgg
     4381 acttatcgga aggaatggaa acatcatgaa gaaaatcgga ctgttcggcg gcactttcga
     4441 cccgatacac aacgggcatc tccacatcgc ccgcgccttt gccgacgaaa tcggcttgga
     4501 cgcggttgtt ttcctgccga caggcggccc gtatcacaaa gacgcagcct ccgcttccgc
     4561 cgccgaccgc cttgccatgg tcgaactggc gacggcagaa gacgcgcgtt ttgccgtcag
     4621 cgattgcgac atcgtccgag aaggtgcaac ctatactttt gatgccgtcc aaatcttccg
     4681 ccagcagttc ccatccgcgc aactctggtg gctgatgggc agcgacagcc tgatgaagct
     4741 gcacacatgg aaaaaatggc agatgctcgt gcgcgaaacc aatatcgccg tcgccatgag
     4801 gcagggcgat agcctgcacc aaaccccgcg cgaactgcac gcgtggctgg gcaagtccct
     4861 tcaggacggc agcgtccgca tcttgtccgc cccgatgcat aatgtgtcgt caacggaaat
     4921 ccgccgcaac cttgccagcc aaggcgtttc agacggcatc ccgcctgccg ccgcacgcta
     4981 catccgcgaa cacggtttgt atgaaaaata aagtcaaatc agtaagaaac ggctataatg
     5041 ccgtctgaaa acatcccgtt aggaaaataa tgaacgaaca agaactgcaa gacctgcaaa
     5101 aaatggtcgg ggtcgccgtc aacgccctcg aagacatcaa agccaaagac atttccgttc
     5161 tcgaaacgca agacaaaact tcgctgtttg ccagaatgat tatcgccagc ggcgacagta
     5221 cgcgccaagt caaagcactg gccaacaacg ttgccgtcga tttgaaagaa gccggttttg
     5281 aaatcctcag caccgaaggc gacagcggcg aatggacgtt ggttgatgca ggagacctcg
     5341 tcgtccacgt tatgctcccc gccgtgcgcg acttctatga tatcgacacc ctttggggcg
     5401 gcgagaaacc gagtttccac gccggaatgc agaagccgtg gcacgcggca gactgattcc
     5461 cgatgccgtc tgaatgttca gacggcattt tcctgtaagg gagaaagcat tgaacatcac
     5521 cgttttggca gtcggcacca aaatgccgcg ctgggttgat gaggccgtcg ccgaatacgc
     5581 caaacgcttc ggacgcgacg tcgcctacgc actcaaagaa atcaaacccg aaaaacgcgg
     5641 cgcgggcgtg aatgccgccc aaggtatggc ggcggaagaa aaacgcatcc ttgaagccat
     5701 tccgcaaggc gcgttcctcg tcgttcttga cgaacgcggc aaagcaccga cctccgtcga
     5761 gctggcggaa cacctcaaaa gctggcagca aaacggcgaa cacgtctgct tcgtcatcgg
     5821 cggcgcggac ggaatgaccg accgcctcaa acaacaagcc cgtataatga tgcgcctgtc
     5881 cagcctgacc ctgccgcacg ggatggtgcg cgtccttctg accgagcagc tctaccgcgc
     5941 cgcctccatc ctgcacaacc atccctatca tcgggaataa gaaggctttt gtccgtatcc
     6001 ctaatcggtt aaaattaccc cgttatttct gaacacagca aaggaatccg ctatggcacg
     6061 catggtattc tgcgtcaagc tcaacaaaga agccgaaggc atgaaatttc cgccgcttcc
     6121 caacgaattg ggcaagcgca tttttgaaaa cgtatcgcaa gaagcgtggg cggcgtggac
     6181 gcgccaccaa acgatgctga ttaacgaaaa ccgtttaagc ctcgccgatc cgcgcgcgcg
     6241 cgaatacctg gctcagcaga tggagcagta tttcttcggc gacggcgcgg atgccgttca
     6301 gggatacgtt ccgcaataac ggttttccgt tttgaacaca ggctgtccga aactgcttca
     6361 gacggccttt aaaatacgcc ggacaacttt atcttcacgc aaccggacaa attttgacat
     6421 tgggcaatgt taaaatcccc cattattccc aaccgcgctt tcaggagcag atgatatgca
     6481 acacgacgtt tacgactaca ccgcgcatac ggtttctaaa aacaccgtcc tgcagaaaac
     6541 ctaccgcctg ctcggatttt cattcattcc ggctgccgca ggcgcggcac ttgccgccaa
     6601 tgccggtttc aatttttacg ccgccttcgg ttcgcgctgg atagggtttg ccgtcgtgtt
     6661 ggcgtttttc tacggtatga tccacttcat cgagaaaaac cgttacagca ataccggcgt
     6721 taccctgctg atggtattca cattcggtat gggcgtattg atcggccccg tgctgcaata
     6781 cgcgctccat attgccgacg gtgcgaaaat cgtcggcatt gccgccgcga tgaccgccgc
     6841 cgtcttttta acgatgtccg ccttggcgcg ccgaacccgg ctcgatatga acgcgctcgg
     6901 acgcttcctg accgtaggtg cggtcattct gatggtcgcc gtggttgcca atctgttttt
     6961 gggtattccc gcactcgccc tgaccatttc cgccggtttt gtcttgttca gttccttaat
     7021 gattatgtgg caggtacgca ccgtcatcga cggcggcgaa gacagccaca tcagcgcggc
     7081 actgacactg tttatctcgc tttacaacat cttcagcagc ctgctgaaca tcctgttgtc
     7141 tttaaacggc gaggattgat gtcggtaaaa cgccgtccga ctatgccgtc tgaaaatgct
     7201 tcagacggca ttttactttg ggcatacaat acggatgcac acgcaccaac acacagccga
     7261 ctatgaagcc gggaggaatc aaatgttacc gaatacaccg cgccgcgccg tttatgccgg
     7321 cagtttcgat ccgcccacat tggggcatct gtggatgata cggcaggcgc aatctatgtt
     7381 tgacgaactc atcgtcgcca tcggcattaa ccccgacaaa cgcagcacct ataccgtcgc
     7441 tgaaaggcag gatatgttgt gcgatattac taaaatgttt cccaacgtca gaaccgatgt
     7501 atttgaaaac cgatttctgg tgcattacgc ccgtgaggta gatgcaggat tcatcgtgcg
     7561 cggcatccgt tctgcttcgg attacgaata cgaacgttcc atgcgccata tcaacagcga
     7621 cctcgccccc gaaatatcca ccgtattcct catgccgccg cgcgaaatcg ccgaagtgtc
     7681 gtccactatg gtcaaaggtt tggtcgggcc ggaaggctgg acggaaaccg tcaaaagata
     7741 tgtgccgccg gccgtgtacc aaaaaatgat tgcagaacat caacacgaaa attgaaccct
     7801 tctatttttg caaggcatat atggatatgt tggaaatttt gttccgccat caagacttcg
     7861 tcgccatcaa caaaccgggc ggcatatccg tccaccaaga cagcggcgag accggactgg
     7921 cccgaacact cgccatacag ttgggcgtag agcgtgtgtg gctgctgcac cgattggaca
     7981 aacagaccag cggcatttta ctgtttgccc tcaacagaga aagcgcgtcc gcccttagcg
     8041 gacagtttgc cggcaaaagc atcaaaaaaa cctatttggc actatccgac cgcaaaccgt
     8101 ccaaaaagca gggttggatt aaaggcggaa tggaaaaatc cagacgcgga atgtggaagt
     8161 tgacgcgcaa tacagaaaat atcgccgtta cccgattcca cagcatcagt atcgccgaaa
     8221 aactgcggct gttcatcctg gaaccgcata cgggcaaaac gcaccaattg agagtggcga
     8281 tgaaaagttt gggcagtccg attctgggtg acagcttata cggcggaaca gaatccgaaa
     8341 ccatgtttct gtatgcatgg aaaatacaat ttgcctacca aaaccgacaa atcgaaattg
     8401 ttgcaccctt aaaaaacgaa tggcagactg aaaatatctc tcatgcgctg gaagaatttt
     8461 atatggcaaa agcaggttaa taaatataaa ttaatttata aacaatatga taaatatttt
     8521 tataaaaatt tttcaagaaa acggttgact gcaaatctct tca
//
LOCUS       DAXHTY010000081         8435 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXHTY010000081 DAXHTY010000000
VERSION     DAXHTY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.081"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..176
                     /locus_tag="ACFNMK_001618"
     CDS             <1..176
                     /locus_tag="ACFNMK_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6364845.1"
                     /translation="DFFEGKGVDVLYMHFHKANEFLGMTRLPTFLCNDVVKNPQVEKY
                     LADYQAHLEKVFG"
     gene            complement(385..1770)
                     /gene="pntB"
                     /locus_tag="ACFNMK_001619"
     CDS             complement(385..1770)
                     /gene="pntB"
                     /locus_tag="ACFNMK_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC6364846.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGSVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALGVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKGANSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNRPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1836..2219)
                     /locus_tag="ACFNMK_001620"
     CDS             complement(1836..2219)
                     /locus_tag="ACFNMK_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC6364847.1"
                     /translation="MTFAYWCILIAYLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2223..3764)
                     /locus_tag="ACFNMK_001621"
     CDS             complement(2223..3764)
                     /locus_tag="ACFNMK_001621"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC6364848.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTDNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAASAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIASINIFGGFAVTRRMLN
                     MFKKG"
     gene            4054..4887
                     /gene="serB"
                     /locus_tag="ACFNMK_001622"
     CDS             4054..4887
                     /gene="serB"
                     /locus_tag="ACFNMK_001622"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC6364849.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SEADAALLGSRQIDHAVLPDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKETGIGVAYRAKPKVRAAADVCINFGGLERVRGLF
                     G"
     gene            4951..6173
                     /locus_tag="ACFNMK_001623"
                     /pseudo
     CDS             4951..6173
                     /locus_tag="ACFNMK_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            6334..7914
                     /gene="purH"
                     /locus_tag="ACFNMK_001624"
     CDS             6334..7914
                     /gene="purH"
                     /locus_tag="ACFNMK_001624"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC6364850.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLGEHVAKMEERGIGNIDLVCV
                     NLYPFAATIAKPNCILEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDILSGTPEIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDICR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDANLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGVRHFRH"
BASE COUNT         1884 a         2309 c         2380 g         1862 t
ORIGIN      
        1 gcgatttctt tgaaggcaaa ggcgttgatg ttttgtatat gcacttccac aaagccaacg
       61 agtttttggg tatgacccgc ctgccgacat tcttatgtaa cgatgtggtt aaaaatccgc
      121 aagtggaaaa atacttggca gattatcagg cacacttgga aaaagtgttc ggctaattaa
      181 aaatccatct tcaacacgga gatggatttt gtttgtttcg ttgattttgt gtcagtttca
      241 gatgtaggtg cttattcgga cgggccgtct gaaaatgttt gccccaatgc aaaaaaaatc
      301 actgcaaacc ttcataaacg gggtttgcag tgattttttc aaatcaaaca gattgaaaac
      361 ctgcgccgaa ttgttcagac ggcattattt tttcagttcg gacagaatgt catccacggt
      421 tttcttcgca tctccgaaac acattacgct gttttcgttg aagaacagcg ggttttgtac
      481 acctgcgtag ccggtattca tcgagcgttt gaagacgacg acttcttttg ccttccatac
      541 ttccaacacg ggcatacccg caatcgggcg gttcgggtcg gtttgggcgg caggattgac
      601 ggtatcgttc gcaccaatca ccaagaccac atcggtttcg gggaagtcgt cgttgatttc
      661 gtccatttcc aacacgatgt cgtaggggac tttggcttcg gcaagcagta cgttcatatg
      721 accgggcagg cggccggcga cggggtggat gccgaagcgc acttctgtgc cgtttttacg
      781 caaaagctcg gtgatttcgg caacggggta ttgcgcttgt gcgactgcca taccgtagcc
      841 cggggtaatg atgacactgt ttgcgccttt cagcatttcg gcaacatcgg cagctttgac
      901 ttctcggtat tcccctatct cttggctgcc ggaagatgat gtgccgctgt cgctgccgaa
      961 accgccggcg attaccgaga caaacgaccg gttcatggct ttgcacataa tgtaggacag
     1021 aatcgcgccg cttgagccga ccaacgcgcc ggtaacgatg agcaggtcgt tggagagcat
     1081 aaagcctgcc gctgcggccg cccagccgga gtaggagttg agcatggaca cgaccacggg
     1141 catatctgcg ccgccgatgg aggcaaccaa gtgccagccg aatgcgaggg caatcagggt
     1201 cataatcagc aggatgaagc cgctgccgtc aatgccgacg aatacgagca gcaacacaaa
     1261 cgatacgcca agtgccagtg cgttgagctt gtgtttggcg ggcagttgca gcgggctgct
     1321 gctgattttg ccgttgagtt tgccgaatgc gaccagcgag ccggtaaagg ttaccgcgcc
     1381 gatgaagatg cctaaataca cttcgaccag atggatggtg tgcatatcgt gcgaaacgct
     1441 gcccggcgcg atatagctgt tgaagccgac caaaaccgcc gctaggccga cgaagctgtg
     1501 cagcagggca atcagttcgg gcatttcggt catttccacc tttttggctt tatagatgcc
     1561 gattgccgcg ccgatgagca tggcgatgat gatccagccc agtccgtggg tattgtcgga
     1621 aaaaacagtt acaaaaaggg cgaccgccat accggcgata ccggaatagc agccctgttt
     1681 ggcggtttcc tgtttggaca gccccgccag tgagaagatg aataaaattg cggcaacgat
     1741 atacgccgct gttacgagtc ctgaagacat ggaaattctc cgattttcga tgatttgttt
     1801 tcaatgccgt ccgaactgtt cagacggcat ctgtttcaag cagccgcgac aaacagcccg
     1861 acggtgcagg caaatccgcc cgcccacatc agcgagcgca atgctgcttt gtctgcgatg
     1921 tagcaccaga taaaggcgag gcggaacagg atgaacaggc cggcaagcgt gttgacggtt
     1981 gcttgtccgg cattgccggt tgcgtgtgcc gtcaaaacgg cggcggcaaa cggtgcaaaa
     2041 gcttcaaaac cgttttgctg cgcagcgtgg gcacgggcgg ctgcgccttg cgtgtgcgcc
     2101 agaaaatcgc gcggattgtg gttgtcttta aaccggaatc cgcccgcttt tttggcatac
     2161 gccgcacaaa aaagcggcaa taggtaggca atcagaatgc accaataggc gaaagtcatg
     2221 gcttaccctt tcttaaacat attcagcata cgccgtgtta ccgcaaagcc gccgaagatg
     2281 ttgatgctgg caatcaggat ggcaacaaac gacagcagcg aaacaaagcc gttgccctga
     2341 ccgatttgca gcagcgcgcc gacgacgatg atgccggaga tggcattggt taccgacatc
     2401 agcggtgtgt gcagcgagtg gctgacgttc caaacgacat agtagccgat gacgcaggcg
     2461 aggacgaaca cgataaagtg gttcaggaat gctgcgggtg cgaccgcgcc gacccacagc
     2521 accaatacgg cggcgatgac ggcgggcgcg agttttttcc acagggaaac aggtttcggc
     2581 tcgggcttgg cggcagacgc ggctttttca gacggcgttt gctgcggctg ggcggaaact
     2641 tgaattggcg gaggcgggaa ggtgatttcg ccgtcgtggg taacggtcat gttgcggata
     2701 atcacgtctt cgaagtccag cgtgatttcg ccgtctttgt tcgggcttaa cagcttggtc
     2761 aggttaacca agttggtggc gtaaagttgg gaagactgtc cggcaaggcg gtttgccatg
     2821 tcggtgtaac cgatgatttt cacgccgttg tcggttacgg acaattcgcc cgggcgggtg
     2881 agttcgcagt tgccgcccgt cgccgccgcc aaatcgacga tgacggagcc ggatttcatg
     2941 ctttcgacca tttctttggt aatcagcttg ggcgcgggtt tgcccggaat ggcggcggtg
     3001 gtgatgatga tgtccacttc tttcgcctgt tcggcaaata gcttcatctc ggcggcgata
     3061 aattcgtcgc tcatcacttt ggcgtagccg tcgccgctgc cgcccgattc ttgcgggaag
     3121 tcgagtttca ggaacttgcc gcccatcgat tcgatttgtt ccgccacttc caagcgggta
     3181 tcgaatgcgc gcaccactgc gccgagcgag tttgccgtac cgattgccgc cagacccgcc
     3241 acacctgcgc caatcaccaa aacctgcgcg ggcggcactt tgccggcggc ggtgatttga
     3301 ccggtgaaga aacggccgaa ggcgttggcg gcttcaatca cggcgcggta gccgctgatg
     3361 tttgccatcg aagacaaagc gtccaaagcc tgcgcgcggg aaatacgggg caccatgtcc
     3421 atcgccagcg cattgacttt cttggcgcgc aaggcttcga ccaaagcctc gttttggcgc
     3481 ggccacagga agctgacgat ggtttgacct tcgttcaaaa gcggcagctc gccttcggac
     3541 ggcgcgttaa ccttataaat caaagggcag gcccaaaccg ccgctttgtc ggcaacggtt
     3601 gcgcctgctg tttggtaagc ggcatcgtcc aaacttgccg ccaaacctgc accgctttcg
     3661 acaacggttt caaagcccag tttgcccagc agggcgacgg tggcgggcgt acaggcgacg
     3721 cgggtttcgc cggataatga ctcgcgtggg atgccgattt tcatctctga atcctttttc
     3781 gggttgttta tatgtatcgt gggttaaatt taaatcgggg cagggcggag caacgccgta
     3841 ccggtttaaa accgattcac ttcaaatgtt aatatatttt agataatccc cttataacga
     3901 attttcatca ggctggcaat agttgcggca ttttcccgtg ttgtccgaca catactgccg
     3961 tttgtttttg aagccgtttt ccgattgcag tcccgcccgc cgtaacattc tttgccgttt
     4021 cactatataa tccgcatttt ttgagccacc gccatgccgc aagccctcgt cctccaattt
     4081 ccctccgccg aagccctgcc ttccgacttc ccctcccgcc tgcccgaacc tgattgcgcc
     4141 gatgaaaagc gtatgcgttt tattgttgaa gaagggtttt ctttaagcga ggcggacgcg
     4201 gcgttgcttg gcagccgtca aatcgaccac gccgtgttgc cggatatgga ttttggcgaa
     4261 ctcggtttga ttgtcagcga tatggattcg acgctgatta ccatcgaatg cgtcgatgaa
     4321 attgcggcag gcgtgggttt aaaagacaag gtagcggaaa ttaccgagcg ttcgatgcgc
     4381 ggagagttgg atttcgagca gtcgttgcgc agccgcgttg cgctgttggc gggattggac
     4441 gaacagattt tggcggacgt ttatgaaaac gttttgaagc tctcgcccgg tgcggaattt
     4501 ttgttggacg aatgcaaaag gcacgatgtg aaattcctgc tggtgtccgg cggcttcacg
     4561 ttttttaccg aacgtctgca acaacgcctc ggcttcgaat accaacacgc caatgttttg
     4621 gaaattgaaa acggcaggtt gaccggccgt ctgaaaggca gaatcatcga cgcgcaggca
     4681 aaggcggatt tattgcgcga ataccgcagc cgcctcggat tgcagccaca tcaggtgttg
     4741 gcgatgggcg acggtgcgaa cgatattccg atgctcaaag aaacgggcat aggcgtggct
     4801 taccgtgcca aaccgaaagt gcgggcagcc gccgatgtct gtatcaactt cggcggtttg
     4861 gagcgtgtac gcggcctgtt cggataggcg gataggaaac ggatgtcgtc cgaaaggttt
     4921 tcagacggca tttgaacggc aggaacgaca gtgggacgca gaaaactttg gtttgccctg
     4981 gcagcagcag gcgttatcgg cggtttggtc ggcattgtgc tgacggaact gatgcacttc
     5041 atacagcata cggcaacggt tatggcgcgg acggcgtgta cacttcgttc cgcgaaggcg
     5101 tggcacaggc ttccggtatg cggcgcgttg ccgtgctgac gctgtgcggc gcggtcgcag
     5161 gcagcggctg gtggttgctg aaacgtttcg gcaagccgca aatcgaaatc aaagccgcct
     5221 tgaaacagcc gttgcagggg ctgccgtttc tgacgacggt tttccatgtt ctgctgcaaa
     5281 tcataacggt cggactcggt tcaccgctcg gacgggaagt cgccccgcgt gaaatgactg
     5341 ccgcgttcgc ttttgccggc ggcaggcggt tgggtttgga tgaagacgaa atgcggctgc
     5401 tgattgcttg cgcttcgggt gcgggtttgg cggccgtgta taacgtgccg ctcgcctcca
     5461 cactttttat cctcgaagcc atgctgggcg tgtggacgca gcaagccgtc gccgccgcat
     5521 tgctgacttc cgtcatcgcc accgccgtcg cgcgcatcgg cttaggcgac gtgcagcaat
     5581 atcatcccgc caaccttgcc gtaaacactt cattactttg gttttccgcc gtcatcggcc
     5641 cgatactggg cgcaaccgcc gtctttttcc agcgcaccgc ccaaaaattc cccttcatca
     5701 agcgcgacaa catcaaaatc attcccttgg cagtctgcat gtttgcgctc atcggcgtga
     5761 ttgccgtttg gtttcccgaa attttgggca acggcaaagc aggcaaccaa ctgacctttg
     5821 gcggtttgac cggttggcaa cacagccttg agctgaccgc cgtcaaatgg ctggttgtct
     5881 tgatggcgtt ggcggcaggc gcatacggcg gactgattac gccgtctatg atgctcggca
     5941 gcaccatttc ttttgcagcc gccgccgcgt ggaacagcgt tttccccgaa atgccgtctg
     6001 gaagcgcggc agtcgtcgga gcggcggtgt ttctcggcgt atcgctcaaa atgccgctga
     6061 ccgccattgt gttcgtattg gaactgacct acgcgccgct gtccctgctg ctgcctttgt
     6121 gcatcggcat ggcgggggca ttgggaacat cgggaaaaat gggcttcaaa taaatgccga
     6181 caaatgccgt ctgaaaggtt ttcagacggc atttctttgt aaattgtcag caatatctgc
     6241 aaaaacctac tgccaaaaaa ctagaaatag cggataatac cgccccgaaa aagttcccct
     6301 ttccccatcc aactgtcaga aaaggaagcc ccaatgtccg tcatcaaacg cgccctgatc
     6361 agcctgtccg acaaggcagg cgcggtcgaa tttgcccaaa ccctgaccaa gctcggtgtc
     6421 gaaatcctct ccaccggcgg cacggcgaaa ctgcttgccg atgcgggcgt tcccgtgatt
     6481 gaagtcgccg actataccgg cttccccgaa atgctcgacg gccgcgtgaa aacgctgcat
     6541 ccgaaaatcc acggcggtat tttgggtcgg cgcgatttgg gcgaacacgt cgccaagatg
     6601 gaagaacgcg gcatcggcaa tatcgacctt gtgtgcgtca acctttatcc cttcgccgcc
     6661 accatcgcca aaccaaactg catactggaa gacgcgattg aaaacatcga catcggcggc
     6721 ccgaccatgg tgcgttctgc cgcaaaaaac tggaaacacg tcgccatcgt taccgacacc
     6781 gccgacttcc ccgccatcgc cgccgaactc gaagccaaca acggcgcgtt gagcgacaaa
     6841 acccgcttca acctgtcgcg caaagcgttc agccacaccg cccaatacga cggcatgatt
     6901 tccaactacc tgacctcgct ttcagacgac atcttgagcg gcacgcccga aatcggtgaa
     6961 ttcccgagcc agttcaacca aagctggatt aaagtgcaag acatgcgcta cggcgaaaac
     7021 ccgcatcagc gcgccgcgtt ctaccgcgat attgaccccg ccgcaggcag cctttccgcc
     7081 tacaaacaac tgcaaggcaa agaattgtct tacaacaaca tcgccgatgc cgatgccgca
     7141 tgggaagccg tcaaatcctt tgacgcgcca gcctgcgtga tcgtcaaaca cgccaatccg
     7201 tgcggcgtcg ccgttgcagc cgatacattg accgcctaca aactcgccta cgtcaccgac
     7261 accaccagcg cgttcggcgg catcatcgcc ttcaaccgcg aagtggacgg cgaaaccgtc
     7321 aaacagatta ccgacaacca gtttatggaa gtcttgatgg cgccgaagtt taccgccgaa
     7381 gccctcgaaa tcgccgccgc caagaaaaac gtgcgcgtat tggaagtgcc gcttgaggca
     7441 ggcgcaaacc gcttcgaact caaacgcgtc ggcggcggac tgttggtaca aacgcccgac
     7501 atctgccgca tcagccgcgc cgatttgaaa gtcgtctcca aacgccaacc gaccgagcag
     7561 gaatggaacg atttgctgtt cgtctggaac gtcgcaaaat acgtcaaatc caacgccatc
     7621 gtcttcggca aaggcggtca aacctacggc atcggcgcag gccaaatgag ccgcgtggac
     7681 agcacccgca tcgccgcccg caaagcgcaa gatgccaatc tcgacctcaa cggcgcatgt
     7741 gccgcttccg atgccttctt ccccttccgc gacggcgtgg acgtgattgc cgagcagggc
     7801 atcaaagcca tcatccatcc cgcaggctcc atgcgcgacc aagaagtctt cgacgcagcg
     7861 gacgaacacg gcatcgccat ggtcgtaacg ggcgtgcgcc atttcaggca ttaacgcaga
     7921 taaacaaggc aatgccgtct gaagggcttt cagacggcat tttgcgctat tttgtgaagg
     7981 tagggatgac ggttcgggta ttcctgacag ggtggatttt caaggtgttg tatagggtgt
     8041 aggaggattc gtaaaaggtg ggatgcaggg tgtgcttcag cccgctgcat caaaaatttt
     8101 tggagaaccg gcgggagtcg gcggttttgg tttcggcggg gacggtggaa atgggtaaca
     8161 ttgacggaat cgacggaagc ggtgggctga agcccaccct tgtatattgg aatcactgta
     8221 tcatagcaac aaaccgcccg gccgccaccc gcgcccaccc aaggcacaca accgttgcgt
     8281 agctcaggga gcggcagggc aacccatcga cacaaccgga cagttgccgg acaacacaac
     8341 cgaatgcaag gcaggtttat gatgagtacc caataccatt acgcaggtat agtgaattaa
     8401 atctaaacca gtacagcgtt ggttcgcctt agctc
//
LOCUS       DAXHTY010000082         8269 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXHTY010000082 DAXHTY010000000
VERSION     DAXHTY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.082"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(240..968)
                     /gene="rmpM"
                     /locus_tag="ACFNMK_001625"
     CDS             complement(240..968)
                     /gene="rmpM"
                     /locus_tag="ACFNMK_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC6364851.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1332..2282
                     /locus_tag="ACFNMK_001626"
     CDS             1332..2282
                     /locus_tag="ACFNMK_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC6364852.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2395..3129)
                     /gene="fnr"
                     /locus_tag="ACFNMK_001627"
     CDS             complement(2395..3129)
                     /gene="fnr"
                     /locus_tag="ACFNMK_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC6364853.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3334..4755
                     /gene="hemN"
                     /locus_tag="ACFNMK_001628"
     CDS             3334..4755
                     /gene="hemN"
                     /locus_tag="ACFNMK_001628"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC6364854.1"
                     /translation="MKIIQIQNKHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTVAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5108..6682)
                     /locus_tag="ACFNMK_001629"
     CDS             complement(5108..6682)
                     /locus_tag="ACFNMK_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC6364855.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            7007..8107
                     /locus_tag="ACFNMK_001630"
     CDS             7007..8107
                     /locus_tag="ACFNMK_001630"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC6364856.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPGR
                     LRRKLLDLQDTGADELHRSRMLSQELSRLYAQTVGELLNKQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADTRQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
BASE COUNT         2097 a         2325 c         2011 g         1836 t
ORIGIN      
        1 acggcaatgg gacacaaacc tgaatctgaa tctgaatctg aatctgaatc tgaatccgaa
       61 tccgaacctg cacctcgatt ctgacgatag ccgtttcaga aaaccaccca aaaaaacaaa
      121 ccccgcaaaa gcgggggttt gtttcacatc atcgatgccg accgacgttt cacgaatatt
      181 gcaacagtac ctggtacaaa atccactaac ctcatgcatc ggcaagatat tgcctagcct
      241 tagtgttggt gatgattgtg tgccggcaca acctgacggg ttacgatgct gcggattttc
      301 acatccacac ggcggtcagg ttcgatacat gcaatcagag cctcacgttt tttggcttta
      361 gagactttcg cacccagttt ggcaacttcg gcttcacaaa cttgagtcat ttgcgcttga
      421 gattcgccca agccgacagc agaaattcta gaaacaggta cgccgttgct gaccaggttg
      481 tttgccacta cgtatgcgcg gcgttcggac agggcctgat tgtatttgtc agaacccata
      541 aagtcggtat ggccttcgac gcggacagat tggacattgg ttcgactcag gcgttgcgcc
      601 aatactttca ggttgtcttg agcttcggcg cgcaatgaat ccttatcgaa accgaacagg
      661 gttttggcag acagggaaat ggtttcatca acatattgcg gagcctgctc cacaacgacg
      721 acaggcgcgg gtgcgggttc gggttctggc tcgggttcgg gggcagcaac cgcatcgccg
      781 cattctacgc gaccttggct tgctttatca aagtaggcgt ttttccagca ttcgccatag
      841 ttgttgcgta cgatttcgtt cgactggccg cttacggtgt aaccctgaac ggacgcctcg
      901 cccgcaacag cagtgccgga agcgagcaat gcaacgaata atgcgcttaa tttcagctgt
      961 ttggtcattt tattccctca ttaaatttgt acagcagata cagaagcaac tgctgaattg
     1021 ccaacataca ggccgctgta gaaacatggc ggcaaatcgg atatttcagt ctccactata
     1081 aagaagcagg gggaggactg tcaatactca acgcctgaaa acaaccgtaa aacagcctgt
     1141 ttcctgatag tctatcatgc cgaaaaatca attccgtgtc attactttag ggggattttt
     1201 tccatatcgc acgattgccg ttgcacaaaa acaacaaaca caacggcaaa gccccatacc
     1261 gtaccggcaa gaaaatatga taaattataa acaatgttca gccacccgac acagacccac
     1321 accgacccgc catgaaatta caacaattga aatacgcctt agaagtttac cagcacaacc
     1381 tgaacgtttc cgaagcggcc gaagccttgt ttacttcgca acccggcatc tccaaacaaa
     1441 tcaaattgct ggaagaagaa atcggcattc agatttttat ccgcagcggc aagcgcgtgg
     1501 tttcggtctc gcagccgggc aaggtggttt tggatattgc agaacgtatt ttgcgcgatg
     1561 ttcagaacat taaaaatatc ggcagcgagt ttaccggaca ggacagcggt tcgctgacgg
     1621 ttgccacgac gcatactcag gcacgctatg ccctaccctt gattgttgcc gattttgtga
     1681 aacgctatcc gaaagtcaac ctgaccatca aacaggggag ccctgccgcc atcgcacgta
     1741 tggttacctc aggcgaggcg gacttggcga ttgttacgga acggatagac gaccatcccg
     1801 aactgggaaa acttccctgc tatgactgga ctcatgcggt tatcgtaccg aacgaccacc
     1861 ccttgctcga atgcagaaac cccctccgca ttgaagattt ggcgaggttt ccgctgatta
     1921 cttatgaatt tgcattcaat gcgggcagca gcatcgcgcg ggcattttcc aaagcccgtt
     1981 tggaacagcc ggatgtcgca ttggcggcgg cggatacgga cgtattgaag acttatgtgc
     2041 gcttgggttt gggcgtggga ctgatggcga aaatggcgta caacccggat acggacggcg
     2101 atttgcagct tgtggatgcg gcacacctgt tcgagccgtc gccgacgtgg attgctttgc
     2161 gcagcgatac ttatttgcgc ggatatgcct acgactttat ccaagcgttt gcgccgcacc
     2221 tgacacgcga gaaggtggat aggattctgt acacgcccat cagcgaggat ttttcgattt
     2281 aggcggctgc cggttttcag acggcacttt gcggcagata caacaaacag gacagatgtt
     2341 ttcgtctgcc ctgtgtttat tgagaatgcc gtctgaaatg ttcgtacggg ttaatcaaat
     2401 ggcgtgcgaa caaccggata ccatcttctt caacacctgc agattgagga tcttgatgtg
     2461 tttgtgttcg acggaaatca atccttcctg atggaacttg gataatgtgc ggctgacggt
     2521 ttcaagtttc agcccaagat aactgccgat ttcttcgcga gacattctta agatgaagtc
     2581 gttggcagca aaacctcggg aataaaggcg ttgggaaagg ttcagcagga aggcggcaat
     2641 ccgctcttcg gcgcgcatat tgcccaacag cagcataaca ccttggtcgc gcacgatttc
     2701 acggctcatc atgcggaaga agtgcgtacg caggctgggg atgttttgcc ccagttcttc
     2761 gatgtgggta aacggcagtt cgcacacttc gctgtcttcc aaggcgaccg cgtcgcaact
     2821 gtgcacatgg gaacagatgc cgtccatgcc gatgagttcg cccgacataa agaaacccgt
     2881 tacctgatcg cggccgtcct gactggcgac ggttgttttg aagaagcccg aacggatggc
     2941 aaagagcgag gtaaaggctt cgccgacaca gaacaggtat tcgccctttt tcaggcggcg
     3001 gctttgacgg atgacggcat cgagttggct gagctcgttg ggcagcagcc cgacaggcag
     3061 gcagagttcc cgcaaagaac aggaagaaca cagcgttttc atctgatgtg tagtattatg
     3121 cgaagccata ccgtaccttt ttgtgcgctt tgccccatca tgattatagt ggattaaatt
     3181 taaaccagta cggcgttgcc tcgccttgtc cttatttaaa tttaatccac tatatgtgct
     3241 tattgacaca tatcaagaca ggtttatcat actgtggcat tctaccaaac tatccaaaat
     3301 ttgactaaca tcataaaatc gcacacacac accatgaaaa tcattcagat acagaacaag
     3361 cacaatgtaa acgatgaccg ccccgagttt gaccgcgcgc tgattgccag cctgcccgcc
     3421 agcggtccgc gctacacttc ctaccctacc gccgaccgtt tccatgacgg tttccgcgaa
     3481 ggcgaatata tcaaagcttt acatttgcgc ggtatgggcg cgttaaacaa accgctttcc
     3541 ctttacattc acattccgtt ctgcaacacc atctgctact actgcggctg caacaaaatc
     3601 atcaccaaag acaaaagccg cgccgatgcc tacatcgaat atcttgaaaa agaaatggaa
     3661 ctgctcgctc cacatctgaa cggacggcac cagcttgccc aactgcactt cggcggcggc
     3721 acgccgacct ttttgagcga cgaacagatc gaacgtgtct tccgcatgat acgcaaacat
     3781 ttcgagttaa tccccaccgg cgaatactcc atcgaaatcg acccgcgcaa agtcagccgc
     3841 gacaccgtcc tcatgctcgg cagactcggc ttcaaccgca tgagcatcgg cattcaggat
     3901 ttcgacccca aagtgcaggc agcggtcaac cgcatccaaa gttacgaaga aaccaaagaa
     3961 gtcatcgatg cggcgcgcga agcggggttc aaatccgtca gcgtcgattt gatttacggc
     4021 ctgccgcacc agacttcgga aagcatcaaa accaccatcg ataccgtttt gtcgctcgat
     4081 cccgaccgcc tcgcccttta tcactacgcc cacctgccgc acgtgttcaa accgcaacgc
     4141 cgcatcgata ccgtcgccgt tcccgacagc gaagagaagc tcgatatgct gcaatactgc
     4201 gtccaaaccc taaccgaacg cggctacgtc ttcatcggca tggaccattt cgccaaacct
     4261 gacgacgaac tctccatcgc cctcaaagaa ggcttcctcc agcgcaactt ccaaggctat
     4321 tcgacctacg cggattgcga tttggtcgcc atcggcgtgt cgtccatcgg caaaatcggc
     4381 agcacctatt cccaaaacga acgcgacatc gatgcctact atgccgccat cgacgaaggc
     4441 agactgccca tcatgcgcgg ttatcagctc aatcaggacg acatcctgcg ccgcaacatc
     4501 attcaggatt tgatgtgccg tttcgcgctc gactatcgga tttacgaaag tatgttcggc
     4561 atcccgttcg accgctactt caaagacgaa ctggcggatt tggaaaaact cgccggcttg
     4621 ggattggtgc gcctgaacag ccacgggctg accgttaccc cgaaaggacg cttcctcatc
     4681 cgcaacatcg ccatggtctt cgattaccac ctgcgccaca aagaaaccaa ggcgaaatat
     4741 tcgcaaacgg tgtgattgcg tctaacgtac aaatgccgtc tgaaaggctt tttcagacgg
     4801 cattttgctg ccggcaggat aagtgttttc aagaacaggc ggcggcatat cataacgttc
     4861 cgcacctttg tgtccgaccg ttccgaaacc aagatatagt ggattaacaa aaaccagtac
     4921 agcgttgcct cgccttgccg tactagctgt actgtctgcg gcttcgtcgc cttgtcctga
     4981 tttttgttaa tcactatacc aaacggcata tcccgacaga acagattgtg cataaggcac
     5041 aagcccgcac attccatcaa caaaatgccg tctgaacacg ggttcagacg gcatcagtat
     5101 tttacaatca gaatactgcc tgtaaaacca agtagcagac aaccgacaat acggcggcgg
     5161 caggcagggt aatgacccac gccaaaccga tgggcttcat cagtttccag ttggcattgc
     5221 ggttgaccag accgataccg agtaccgcgc cgaccaagat atgcgtactg gacacgggca
     5281 gccccatcag cgacgcgccc atcacgacgg atgcggcgga cagttcggcg gtaaaacccg
     5341 aagcaggatg catttccgcc aaactcgtac cgacggtttt aatcacctct ttaccgacaa
     5401 accacaaacc gacaatcagc gcgataccga aagtcagcat cgcaatcggg gggacgacat
     5461 tttgcgcggc aacgctgttg gtacgcaaaa catccataat cgcggcaaac ggaccgatgg
     5521 cgttggcgat atcgttcgcc ccgtggctga atgcgaagcc gcaggcggta aagacctgca
     5581 tccatgaaaa catctgaaag gtcgatttgc ccaagtcttt acgcttgagg cttttggcaa
     5641 aaacaaacgt ccccatccac accgccgcgc ctatcataaa gatggtcagg aagctgttga
     5701 cgttgctcat ccccaaatgc aggtttttca agcccttgaa aatcagcata gcggaaatca
     5761 tcatcgcgcc gaacgaagcg ataaagggaa tccaagaatg cagtgccttg taggaatcga
     5821 cattgttttt acggttgtcg aacgcataaa gaccgcggta atactccgat tgcagctctt
     5881 gcggatcgaa ttcgggttcg tcgtaaattt gcgcgtcgtg cgccattttg gtggcgtact
     5941 cgactttttc ggcttcggac aaaccctcga aaaacaggcg gtgccgttct ttataggcct
     6001 ttttttcctg cttgatgccc ttgagcgtgc cttccgccca agcgttgtaa tctaagacgt
     6061 ttttcttgac gcgcgaaaac aggaaatagg acaccgcgcc gcccaacacg ggcgacaata
     6121 cccaagaaac accaataccg cccagcttgc cccaacgtat caaatcgccc gatgcggcat
     6181 cgttcattac cgccatacat accgcgctgc cgacaatgcc gccgataatg gaatgggtgg
     6241 tagataccgg aagccctttt ttcgaggcaa acaacagcca caacgccgcc gccaaaagcg
     6301 cggacatcat aataaacaca aactgtatgg gttcgaaatc aacacccttc aaatcgacga
     6361 tgcctttgcg tatggtattg gttacctcac cgcccgcgat gaccgcgccg ctgacctcaa
     6421 ataccgccgc aatcagcaaa gcctgcggga tagtcagcgt acccgcaccg acgctggtgc
     6481 cgaaagaatt ggcaacatcg ttgccgccga tgttgaacgc cataaacacg ccgaacatcg
     6541 tggcaataat gaataaggcg gcgtaattgt aatgggtata acccaagccc caataaataa
     6601 aatagccgac tgtacctacc agcatggcgg caaagacggc gttgatggtt ttaacatttg
     6661 cgctcatttg gatttgagcc atggcaatct tcctttaatg tggtggaaat catgagtgtt
     6721 gccgacactg tactgctgcc tccggtcgaa ctgccgtttc gggatatggc agacgcatcc
     6781 ttccttctga accgcatccg aataccccca aacctgaccg aatgcaaaac tgctgccgga
     6841 aacggtttgt atgacacttc tactactgga tgttgcgggc gcattataat atttttccat
     6901 ccgtcttgaa acatttattt acactttatt tacactgcgg cggcaaatcg gtatacgagc
     6961 gtcaatacac gttaaaatgg cgttttgcac cagtttggga gtgatgatgg aaacacagct
     7021 ttacatcggc atcatgtcgg gaaccagtat ggacggggcg gatgccgtgc tggtacggat
     7081 ggacggcggc aaatggctgg gcgcggaagg acacgccttt accccctacc ccggcaggtt
     7141 acgccgcaaa ttgctggatt tgcaggacac aggcgcggac gaactgcacc gcagcaggat
     7201 gttgtcgcaa gaactcagcc gcctatatgc gcaaaccgtg ggcgaactat taaacaagca
     7261 aaacctcgcg ccgtccgaca ttaccgccct cggctgccac gggcaaaccg tccgacatgc
     7321 accggaacac ggttacagca tacagcttgc cgatttgccg ctgctggcag aacggacgcg
     7381 gatttttacc gtcggcgact tccgcagccg cgaccttgcc gccggcggac aaggtgcgcc
     7441 gctcgtcccc gcctttcacg aagccctgtt ccgcgacgac agggaaacac gcgcggtact
     7501 gaacatcggc gggattgcca acatcagcgt actccccccc gacgcacccg ccttcggctt
     7561 cgacacaggg ccgggcaata tgctgatgga cgcgtggacg caggcacact ggcagcttcc
     7621 ttacgacaaa aacggtgcaa aggcggcaca aggcaacata ttgccgcaac tgctcgacag
     7681 gctgctcgcc cacccgtatt tcgcacaacc ccaccccaaa agcacggggc gcgaactgtt
     7741 tgccctaaat tggctcgaaa cctaccttga cggcggcgaa aaccgatacg acgtattgcg
     7801 gacgctttcc cgttttaccg cgcaaaccgt tttcgacgcc gtctcacacg cagcggcaga
     7861 tacccgtcaa atgtatattt gcggcggcgg catccgcaat cctgttttaa tggcggattt
     7921 ggcagaatgt ttcggcacac gcgtttccct gcacagcacc gccgaactga acctcgatcc
     7981 tcaatgggtg gaggcagccg catttgcgtg gttggcggcg tgttggatta accgcattcc
     8041 cggcagcccg cacaaagcaa ccggcgcatc caaaccgtgt attctgggcg cgggatatta
     8101 ttattgagcg caaataccgt ctgaaagcca tttccgccgc tcagacggca ttttcgcccc
     8161 ttttgtttac aaacccttaa aatccattta cacttaaacc cgttcaaaac aaaacaaacc
     8221 ctgccatgaa aaccctgctc ctcctcatcc ccctcgtcct cacagcctg
//
LOCUS       DAXHTY010000083         8253 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXHTY010000083 DAXHTY010000000
VERSION     DAXHTY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.083"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(241..858)
                     /gene="thiE"
                     /locus_tag="ACFNMK_001631"
     CDS             complement(241..858)
                     /gene="thiE"
                     /locus_tag="ACFNMK_001631"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC6364857.1"
                     /translation="MTFLPLKSPLKFYAVVPTADWVERMVEAGADTVQLRCKALHGDE
                     LKREIARCAAACRGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLEKLREYVKQAGSTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
     gene            complement(879..1979)
                     /locus_tag="ACFNMK_001632"
     CDS             complement(879..1979)
                     /locus_tag="ACFNMK_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC6364858.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNASAEHGSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            complement(1976..3199)
                     /gene="cytX"
                     /locus_tag="ACFNMK_001633"
     CDS             complement(1976..3199)
                     /gene="cytX"
                     /locus_tag="ACFNMK_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC6364859.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     regulatory      complement(3266..3370)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3445..4929
                     /gene="tldD"
                     /locus_tag="ACFNMK_001634"
     CDS             3445..4929
                     /gene="tldD"
                     /locus_tag="ACFNMK_001634"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC6364860.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIASRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITGGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSEI"
     gene            4994..5455
                     /locus_tag="ACFNMK_001635"
     CDS             4994..5455
                     /locus_tag="ACFNMK_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364861.1"
                     /translation="MQEQNRKPSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAAFWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVASIGTFAGKNKERKAASAADGTKNDV"
     gene            5445..6266
                     /gene="prmC"
                     /locus_tag="ACFNMK_001636"
     CDS             5445..6266
                     /gene="prmC"
                     /locus_tag="ACFNMK_001636"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6364862.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            6352..7740
                     /locus_tag="ACFNMK_001637"
     CDS             6352..7740
                     /locus_tag="ACFNMK_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC6364863.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLTGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            7741..7965
                     /locus_tag="ACFNMK_001638"
     CDS             7741..7965
                     /locus_tag="ACFNMK_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC6364864.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDTQEPYSLRDEIPPHY"
BASE COUNT         1738 a         2216 c         2518 g         1781 t
ORIGIN      
        1 gtcgaattac tttatttttt gatacgcaac cggccggttg ccgtcattcc cgcgcaggcg
       61 ggaatccatt tttgaaattc ggaaactgtt tttcaaatga tggtttctca agttttaagg
      121 cggattcccg cttgcgcggg aatgacggaa tttgatgata tgctacattt aaagttaagt
      181 atgtttgcga taacgggcta agttttgcct acacggcaat tgaattttct tctttcggtt
      241 ttatccatcc cacaaagcct gaaacgcttt aaccaccgct tcgggatttg ccgcttcggt
      301 tacggcgcgg acggcggcga gtgaggaaac gccggtggcg agtacggctc gggcgttgtt
      361 caaatcgatg ccgccaatgg cgacgacggg cgtgctgcct gcctgtttca catattcacg
      421 cagtttttcc aagccttgag gggcggtggg catttgtttg gtcgtggtcg ggaaaatcgc
      481 gccgctggcg acgtagctgg ggtgcacgga cagggcgcgg tcgagttcgg cgacggagtg
      541 cgtactcaaa cccaaacgca aaccggcagc ggcaatggcg gcgaggtcgg cggtgtccat
      601 gtcttcttgt ccgagatgca cgccgtacgc gcctgcttct atcgcttcgc gccagtggtc
      661 gttgatgaaa agctgcgtac ggctgccacg gcaggctgcg gcgcagcggg cgatttcgcg
      721 tttcaattca tcgccgtgca gggccttgca gcgcagttgc accgtgtcgg cacctgcttc
      781 gaccatgcgc tcgacccaat cggcggtggg gacgacggcg tagaatttga gcggggattt
      841 gagtggcagg aaggtcatgg ggttcctttc agacggcttt aatcttgtct tcggatatac
      901 gccaaaccgc tttcttcatc gcgttcgggt gcatcttttc cgtcaaacag tgccactgcc
      961 aatctgacgg cggcggcggt tacggcgggg gagatcatga aaccgtggcg gaaaaggccg
     1021 ttgatttcga tgaggcggcg tttgcggctg tagcggattt cggggttgtg gtggttgagc
     1081 gtggggcgca ggccggtggc gatttcgagg atgtcggctt cgccgaaggc ggggtggacg
     1141 gcatagagtg cggacaagag ttccaaccct gaacgcacgc tggcgggggc ttggctttcg
     1201 ctttcgattt gggtcgcgcc gatgacgaag acgtggtttt ctttcggggc gatgtagagc
     1261 ggataacgcg ggtgcagcag gcgcacgggg cggttgagcg tgatttcggg tgtgtaaacc
     1321 cgcgccactt cgccgcgtat gccgcgcagg gtgctgccgt gttcggcaga agcattccac
     1381 gcggtttttg cgccgtagcc gcggcagtcg atcagccagt cgtattgggc ttgcaggtct
     1441 tcgggggcac attcgtgttc ccaatggcaa gggacgttca gttcgtccaa agcgtcggca
     1501 agtgcagaca atatttgccg cccgtcgagc tggccttcgg tcggcaggta gatgccgtct
     1561 gaaaaacgtc cgccgagttg cggttcgcgt tcggcgatgt cgtcggcgcg ccaacggacg
     1621 atttcgtcat ccgctacgcc gccgcgtttg agatggcgga cgaactcgct ggataatggc
     1681 ttgtcctgcc cgtgccacac aatcaggctg ccgttttcct gcatcatcgt gtgcgtgttc
     1741 agacggcatc ggatgccgcg ccaaagcggg atgctctgcc tgcccagcct gaccacttcg
     1801 ggcgtggctt cgaccgcttc cgccgcaggc gcgagcatgg cggcggcaac ataggcggcg
     1861 gcgtgttcgc cccggcggca gcctttatcg aaaagtgcaa tctgataacc ttgttctgca
     1921 agctgcaacg cggtcagcct tcccgagagg ccgccgccga ggatggcgat acgggtcatg
     1981 acgggttcct ttgtaaagat tgggtttttt taaagaaaag gcgtaccgat acggtggcaa
     2041 tggcaacggc agacattacg ggggcggtca gaccgatgct gctttcccag ccggacgaga
     2101 gcaggaagcg gtagaggatg aagcccgcaa gccacagaac cagtccggca aagtcaaagc
     2161 cttcaatctc ctcacgccgt ttcaagacga aaaagtcggc aatcaaaacc gccgccatcg
     2221 gcgcaaatac cgagccgata agcagcagga agttttcata ttcggtaacg ggcagcatga
     2281 cggcaagtac cgtgccgatc agggtaacgc cgacagcgac gggtgtttcc gcaaaacgcg
     2341 cggaaatgtt gttcgcactc gcgccggcgg aataggcatc gagaaacgtt gtggtaacgg
     2401 tggagaggac gaccgccaaa atgcctgccg cacccaaacc tgcgcccagc aggatttttg
     2461 ccacgtcggt ttctccggtg aacaacgccg ctgccaaacc caaggcatac atccagcagc
     2521 cggtcagcgt gtaggcgagc gttgccgtca gggttgccgc aaacgggcgg cgcgcgtggc
     2581 gcgtgtagtc ggcggcaagc ggcagccagg aaagcggcat cacggcggac agctcgactg
     2641 ccgttccgaa actcatgccg tctgaaacct gtgcggcggt gctgcctgcc gtggaaaaga
     2701 cttcggcact cagccacaga accgccaaca gcatcagcag catcgaaacg gttttcagcc
     2761 cgcctgtttt gcgtgcgccg aaaaccagcc acagcacaat cagcgcgccg tttgccaatg
     2821 cccaccagac aaaagattcg ccgtcccaca acactttgcc caaagcggag ctgaccgttg
     2881 cgccggcgta aatcatcacc gccgtccagc cggccagttg cagcatattc gccacggaaa
     2941 acagcactga accgcgtttg ccgaacgaca ggcgcacgct ttccatcgag ctgcgtccgg
     3001 tcagtgcgcc gatatacgcc gccgcaaaaa acagcgcgcc gccgacggca tgacccaaaa
     3061 gtagagccgc cagaccgcgt tgccagccca aaggcgcaag cagcgtaccc gtgctgattt
     3121 cggcaatcga taccgccgcg ccgaaccaaa tcagcccgat ggcggcggaa gatgaaggag
     3181 aggaggcatt gcccgacata tgacgtcctt taaaaagaag gaaggtgggc ggggaagtgc
     3241 ggaaaaggga acggaagcct gataaggcat ttgacggttt gtttcctacg caggcattac
     3301 cccgacaggt tctacggatt ttgctttcgc aatctcagcc gcccggcggg cagcactccg
     3361 acaaggagct ttaagtgtac acaaaagcag gggatgttgg caaaattctt acattttcgg
     3421 cggcacggcg catttccata acaaatgccg tataatcaag cccctgattt tttatgcgcc
     3481 gacactatgc atccgaccta ttccgccgta caggcgcggc tgctcgaagc caaccgcctt
     3541 tcccccgaac tgctcgccaa aagcctgtgc atcatcggcg cgcaccacgt cgattacgcc
     3601 gacatctact gccagcgcac cgcttatgaa agctggcatt tggaagaggg catcgtcaaa
     3661 tcgggcagct tccaaatcaa tcagggcgtg ggcgtgcgcg ccgtttcggg cgataaaacc
     3721 gcctttgcct acgccgacag cctgtgcatc gattcgataa accgttccgc ccgcgccgtc
     3781 cgcgcgattg gggcggcagg cggcaaggtg tccgccaaaa tgccgtctga aacgcgcggc
     3841 aagccggttt gttccgcgtc cgaccccatt gccggcctcg attccgccgc caaagtcgcg
     3901 ctgttgaaca aagtggaagc aatcgccaaa gccgccgatc cgcgcatcgt gcaagtgatg
     3961 gccggtttga cctgcgaata cgatatggtt tacctcgccc gtctggacgg caaacacgcc
     4021 gccgacatcc gcccgatggt gcgcctgaac gttaccgtta tcgccaaaca gggcgaacgc
     4081 cgcgaacagg gcagcgcggg cggcggcgga cgctacgact tggcttattt cgatgaaaac
     4141 ttggtgcggc agtttgtcga tgccgccgtc aaacaggcac tcaccaacct cgaatcccgc
     4201 cccgcgcccg ctggcgaaat gaccgtcgtt ttgggcaacg gctggccggg cgtgttgctg
     4261 cacgaagcgg tcggacacgg tttggaaggc gatttcaacc gcaaggggac aagcgtcttt
     4321 tccggcagaa taggcgagcg cgtcgccgcc aaaggcgtta ccgttgtgga tcagggtgat
     4381 attgccagcc ggcgcggttc gctcaacatc gacgacgaag gcaacgaaac ccgccgcacc
     4441 gtattgattg aagacggcat tttagtcggc tatatgcagg acgaaaccaa tgcccgcctg
     4501 acgggtacgc aatccaccgg caacggccgc cgcgaaagtt acgcttccgc ccctatgccg
     4561 cgcatgacca atacctttat ggaaaatggc agctatgagc cggaagaaat catcgcgtcc
     4621 atcgacaaag gcatttacgc cgtcaacttt ggcggcggac aggtggacat caccggcggc
     4681 aaatttgtgt tcagcgcgtc cgaagcatgg tgggtggaag gcggcaggct gcaatacccc
     4741 gtcaaaggcg cgaccattat cggcaacggg cccgaagtgc tgaaacacgt ttcgatgata
     4801 ggcaacgata ccgcgttgga cagcggtgtc ggcgtgtgcg gcaaagaagg gcagagcgtc
     4861 cccgtcggcg tggggcaacc gactttgcgg attgatgccg gactgaccgt cggcggtagc
     4921 gaaatctgac gcagaatccg gcacgatgcc gtctgaaagg ttttcagacg gccttttaca
     4981 ttaggataaa accatgcagg aacagaatcg gaaaccaagt tttcccatag tgatgttgct
     5041 ggtgtcggtt gccctgtgga tagcgtcttt atccaatgtt gcattttatt tgggcaatca
     5101 tggaagcatg gagggtttga ccgttttgat tttggggtcg atatttgctt ctttggatat
     5161 caggtattgt gcggtctatg cgaattatgt ttggttggcg gccattgttt tgctggcgtt
     5221 gcggaagaag gtcgtgcctg tccatgcggc attttggggc ttggcgttgg tggctttcag
     5281 tgtgaaagcc gtatacgtcg atgaagcagg gaatacatcg gatattgtgc gctacggtgc
     5341 aggattttat ttgtggtatg ccgcatttgc ggttgcctcc atcggtacgt ttgccggaaa
     5401 gaataaggaa agaaaagccg catcggcggc agacgggacg aaaaatgacg tttgataaat
     5461 ggttgggctt gtcaaaactg cctaaaaatg aagcaagaat gctgctgcaa tatgtttcgg
     5521 aatatacgcg cgtgcagttg ttgacgcggg gtggggaaga aatgccggac gaagtccgac
     5581 agcgggcgga caggctggcg caacgccgtc tgaacggcga gccggttgcc tatattttag
     5641 gtgtgcgcga attttatggc agacgcttta cagtcaaccc gaacgtgctg attccgcgcc
     5701 ccgaaaccga acatttggtc gaagccgtat tggcgcgcct gcccgaaaac gggcgcgtgt
     5761 gggatttggg gacgggcagc ggcgcggttg ccgtaaccgt cgcactcgaa cgccccgatg
     5821 cgtttgtgcg cgcatccgac atcagcccgc ccgcccttga aacggcgcgg aaaaatgcgg
     5881 cggatttggg cgcgcgggtc gaatttgcac acggttcgtg gttcgacacc gatatgccgt
     5941 ctgaagggaa atgggacatc atcgtgtcca acccgcccta tatcgaaaac ggcgataaac
     6001 atttgttgca aggcgatttg cggtttgagc cgcaaatcgc gctgaccgac ttttcagacg
     6061 gcctaagctg catccgcacc ttggcgcaag gcgcgcccga ccgtttggcg gaaggcggtt
     6121 ttttgttgtt ggaacacggt ttcgatcagg gcgcggcggt gcgcggcgtg ttggcggaga
     6181 atggtttttc aggagtggaa accctgccgg acttggcggg tttggacagg gttacgctgg
     6241 ggaagtatat gaagcatttg aaataattgt ttgcaaaatt ggcgggtttt ccattattgg
     6301 gttatgatgt gtttcgatat gtaaacattt ataggtttgg agcgataaac aatgaatgca
     6361 gtcgtagttg ccgtaatcgt tatgctggtg ctgtcgctgt cgcgcgtgca cgtggtattg
     6421 agcctgacgg tcggcgcgtt tgtcggcggc gcggtggcgg gtatgccgct gcaaaacatt
     6481 gccgatgcgg cgggacaggt cagtcaggcg gggattatcc ccgtgttcaa caaaggtttg
     6541 gaaggcggtg cgaagattgc gctttcttat gcgatgctcg gcgcgtttgc aatggcgatt
     6601 acccattccg gcctgccgca gcagcttgcc ggcgcggtcg tccgcaagct gaaccggggc
     6661 ggtatgcccg acagcgtgcg ttcgggcgag ggcgcggtca aatggctgct gctttccatc
     6721 atccttgtga tgggcattat gagtcagaac atcataccta tccacatcgc ctttatcccg
     6781 atgattgttc cgccgctgct tttggtgttc aaccgcctga aaatcgaccg ccgtctgatt
     6841 gcgtgcgtca ttactttcgg gctggttacg acttatatgt tcctgcctta cggcttcggc
     6901 gcgatttttt tgaacgaaat cctgttgggc aacatccatt ccgccgcgcc gcagcttgat
     6961 gtgaaaaaca tcaacgtaat ggcggcaatg gcgattcccg cgttgggaat gctgaccgga
     7021 ctcctgctgg cgtttgtcca ttaccgcaaa ccgcgcctgt accaaagcaa caatgccgat
     7081 acggcgggca acgccgatgc ggcaaaccgt ccgcagccgt ccgcctaccg cagcctggcc
     7141 gccgccgccg ccattgccgt atgttttgcc atccagttga tgtatgaaga ctcgctggtg
     7201 ttgggcgcga tgctcggttt cgccgtattt atgatgttgg gggtcatcaa ccgcgacaag
     7261 gcaaacgacg tattcggcga aggtatcaag atgatggcga tggtcggctt cattatgatt
     7321 gccgcgcagg gttttgccgc cgtgatgaat gcgaccgggc atatccagcc gctggtggaa
     7381 agcagtatgg cgatattcgg caacagcaaa ggtatggcgg cattggcgat gctggtggtg
     7441 gggcttttgg taacgatggg catcggttcg tccttttcca ctttgccgat tattgccgcg
     7501 atttatgtgc ctttgtgtgt cggtttgggt ttttcgccgc ttgccaccgt cgccattgtc
     7561 ggcacggcgg gggcgttggg cgatgccggt tcgcctgcgt ccgattccac gctgggcccg
     7621 acgatggggc tgaacgccga cgggcagcac gaccacatcc gcgattccgt tatcccgacc
     7681 ttcatccact acaatatccc gctgctgatt gccggctgga ttgccgcgat ggtgctgtaa
     7741 atggacgcgg ttcaagagtt ggaacaccgc atcacagagc tggaaatcca atccgcgctt
     7801 caggaggacg taatcgccgg cctgaacgcg atggtggcgg aattgcggca gacgctggat
     7861 ttgcagcagg ctcagttgag gctgctgtat caaaaaatgc aggacaggaa tcccgacacg
     7921 caagagccgt attccctgcg cgacgagatt ccgccgcatt attgatgtgc cgccgtatcc
     7981 ggatttcctt aaaaaaggct gtgtttgaat attccgccga tgcaaaccta agatgtaact
     8041 ttaatcagtc ggacaaaatg ccttttatcc gatggaaccg ttttccgccc gaacggaaat
     8101 aagcggtttt cccctgtaaa cagataaaac caaccgggta ttcatacaca gccaaaaaaa
     8161 aagccgtccg aacccaaagg ggacagacgg ccaaacacat tacggggaaa acgtcttact
     8221 caatgagtct gcgaaacaga cattgctaaa aca
//
LOCUS       DAXHTY010000084         8106 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXHTY010000084 DAXHTY010000000
VERSION     DAXHTY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.084"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="ACFNMK_001639"
     CDS             complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="ACFNMK_001639"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC6364865.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RTATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHTSAAAAFETGADTVIPLSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELLAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1556..1711
                     /locus_tag="ACFNMK_001640"
     CDS             1556..1711
                     /locus_tag="ACFNMK_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364866.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1708..2139
                     /locus_tag="ACFNMK_001641"
     CDS             1708..2139
                     /locus_tag="ACFNMK_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFC6364867.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVFALAGAFVIGRSRDQGKN"
     gene            2273..6601
                     /gene="app"
                     /locus_tag="ACFNMK_001642"
     CDS             2273..6601
                     /gene="app"
                     /locus_tag="ACFNMK_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC6364868.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYQKDTKHYYHEDYHN
                     PRLNKFVTDAAPVEMTINMNGNTYGNITDYPDRVRIGSGRQYWHKKNGNPQQIFGSYS
                     YRIAGNTHNQVGGSDGHVSLNGDVKKPNNYGPLPTGVSFGDSGSPMFIYDAKKQKWLI
                     NGVLREGNPYQGTHNGYPIVRKAYLTKIQDGDTGASIYDMVGGASYLWEAKELGKSTI
                     SQENNPKFSQMSDIANPSLPKQNKYTPSLDHRKHITFGEYVEGGVGKLTLKSNIDQGA
                     GGLYFEGDFIVSPTQNETWKGAGISISDESTIIWKVNGVANDRLSKIGKGTLHVQAKG
                     ENQGSISVGDGKVILDQQADENNKKQAFSEIGLVSGRGTVQLNADNQFNPDKLYFGFR
                     GGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKESTVTITGNKDIATTGNNNSLDSKKE
                     IAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNGNITQTNGKLFFSGRPT
                     PHAYNHLGSGWSKMEGIPQGEIVWDNDWINRTFKAENFHIQGGQAVISRNVAKVEGDW
                     HLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDDKVIASLTKTDISGNVSLA
                     DHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNGDLSLVGNAQATFNQATLNGNTSA
                     SGSASFNLSNNAVQNGSLTLSGNAKANVSHSALNGNVSLADKAVFHFENSRLTGQISG
                     GKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAAGAQTGSVSDTPRRRSR
                     RSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFRFMSELFGYRSDKLKLAESSEGTY
                     TLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTLQNEHVDAGAWRYQLIRKDGEFR
                     LHNPVKEQELSDKLGKAEAKKQAGKDNAQSLDALIAAGRDAVEKTESVAEPARQAGGE
                     NVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRARRARRDLPQPQPQPQPQP
                     QPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVWTSGIRDT
                     KHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNSARLAHGA
                     VFGQYGIGRFDIGISTGAGFSSGSLSDGIGSKIRRRVLHYGIQARYRAGFGGFGIEPH
                     IGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPYLSLSYTD
                     AASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLEAQHSAGI
                     KLGYRW"
     gene            complement(6710..7432)
                     /locus_tag="ACFNMK_001643"
     CDS             complement(6710..7432)
                     /locus_tag="ACFNMK_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6364869.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGIDYFGTDEDIDFPDPPNMG"
BASE COUNT         2172 a         2224 c         1945 g         1765 t
ORIGIN      
        1 aaccgaacgg atcggattcc cgtttttatg ggataacgga atgttcagcc ggacaccgcc
       61 ccggtgcttt cgatccgttt attttccgat acaaaacctc gaaaaaatca cgccgagcaa
      121 atcatctgcg gtaaattccc ccgtgatttc gccgcacgcg acctgagcca agcgcaaatg
      181 ttccgcaagc agctcgattt gatggttgcc gcacaatgcc gccagcgaca attcttcctg
      241 cgctgctttg agtgcgttga cgtgccgcgt ccgcgccaaa aacagccctt cgctttcgcc
      301 ctgccaaccg gcctcgcgca acaacgtccg tttcagcgcg tccaagccgt cgccggtttt
      361 cgccgacaac ggaatcaccg tatccgcacc cgtttcaaaa gccgctgccg cactcgtgtg
      421 caaatcggat ttgctgtgga tttcgatgcg tttcaactcc ggcggcaacg cgtccaaaat
      481 cgcccgtgtc ttttcattca aaccctcgcg cggatcgacc aacaccagcg cgacatcggc
      541 ttcggatacg gctttgcggc tgcgttcgat gccgatacgc tcgaccacgt cgtccgtctc
      601 gcgcaaacct gccgtatcga caatatgcac cggcacgccg tcaatcagga tacgttccct
      661 gaccgcgtcg cgcgtcgttc cggcaatatc ggtaacaatc gccacttcgt cgcccgccaa
      721 cgcgttcagc aggctggact tgcccacatt cggcgcgccg accaatacga cattcagacc
      781 ttcgcgcaaa atcgcgccct gctgcgcgtt ggcaagcaca tcatccacgg cgcggcgcaa
      841 gccgtccagt ttgccgcgtg cgtctgccgc ttcgagaaaa tcaatgtctt cctcgggaaa
      901 atctaacgtc gcttcgacca gcatccgtaa ggtaatcaag tcttcgacca gaccgtgtat
      961 ccgccgcgaa aaatcgccct tgagtgagcg caacgccaga cgcgccgccg aacggctgga
     1021 tgcgtcaatc aaatccgcca cgccttccgc ctgtgccaag tccagtttgt cgtttaaaaa
     1081 cgcacgtttg gtaaactcgc ccggttcggc aaggcgcgcg cccaactcca aacagcggtt
     1141 cagcagcata tccatcacca ccggcccgcc gtgtccctga agctcgatga catcttcacc
     1201 cgtaaaactt gccggtgcgg caaaaaacag caaaagcccg ctgtcgattg cctgaccgtc
     1261 cgcgtccgta aaatcagcat aggttgcggt acgcggcttg ggcgttttac cgcacaaagc
     1321 ctgcgccatc ggcagcaggt ttttccccga tatgcgtatc acgcccacgc cgccgcgtcc
     1381 gggcgcggtg gcgactgccg caatcgttgg aacgttatcc gacataaaac ccccgaaaat
     1441 tcaaaacagc cgcgattata gcaaatgccg tctgaagtcc gacggttcgg ctttcagacg
     1501 gcataaaacc gcaaaaatgc ttgataaacc cgtccgcctg acctaatata accatatgga
     1561 aaaacgaaac acatacgccc tcctgctcgg tataggctcg ctgctgggtc tgttccatcc
     1621 cgcaaaaacc gccatccgcc ccaatcccgc cgacgatctc aaaaacatcg gcggcgattt
     1681 tcaacgcgcc atagagaaag cgcgcaaatg accgaaaacg cacaggacaa ggcgcggcag
     1741 gctgtcgaaa ccgtcgtcaa atccccggag cttgtcgagc aaatcctgtc cgacgagtac
     1801 gtgcaaataa tgatagcccg gcgtttccat tcgggaccgt tgccgccgcc gtccgacttg
     1861 gcgcaataca acgacattat cagcaacggg gcagaccgca ttatggcaat ggcggaaaaa
     1921 gaacaagccg tccggcacga aaccatacgg caagaccaaa ccttcaacag gcgcgggcaa
     1981 ctgtacggct tcatcagcgt catcctgata ctgctttttg ccgtcttcct cgtatggagc
     2041 ggctaccccg caaccgccgc ctcccttgcc ggtggcacag tgtttgcctt ggcgggtgct
     2101 ttcgtgattg gaagaagccg agaccaaggc aaaaattaat gacaaatcct agggcgtgct
     2161 tcatatccgc ccgaacgccg aaccgcacat ataggcacat cccgcgcgcc gcccggaagc
     2221 ggaagccgcg ccctaccaaa caaacccgaa tcccgtcaga taaggaaaaa taatgaaaac
     2281 aaccgacaaa cggacaaccg aaacacaccg caaagccccg aaaaccggtc gcatccgctt
     2341 ctcgcctgct tacttagcca tatgcctgtc gttcggcatt cttccccaag cctgggcggg
     2401 acacacttat ttcggcatca actaccaata ctatcgcgac tttgccgaaa ataaaggcaa
     2461 gtttgcagtc ggggcgaaag atattgaggt ttacaacaaa aaaggggagt tggtcggcaa
     2521 atcaatgaca aaagccccga tgattgattt ttctgtggtg tcgcgtaacg gcgtggcggc
     2581 attggtgggc gatcaatata ttgtgagcgt ggcacataac ggcggctata acaacgttga
     2641 ttttggtgcg gagggaagca atcccgatca gcaccgcttt tcttaccaaa ttgtgaaaag
     2701 aaataattat caaaaggata caaagcatta ctatcacgag gattatcata atcctagatt
     2761 aaataaattt gttactgatg ccgctccagt agaaatgacc attaatatga atggtaatac
     2821 ttatggaaat ataactgact atcccgatcg tgtgcgtatt ggatcagggc gacaatattg
     2881 gcataaaaaa aatggtaacc ctcaacaaat ctttggtagt tatagttacc gaattgctgg
     2941 taatacccat aatcaagtgg gaggaagtga tggacacgtt agtttaaatg gggacgtaaa
     3001 aaaaccaaac aactatggcc ctcttcctac gggcgtttca tttggagata gtggttctcc
     3061 aatgtttatt tatgatgcaa aaaaacaaaa atggctaatc aatggtgtat taagagaagg
     3121 taatccatac caaggaacac acaatggcta tccaattgta cgtaaagcct atttaacaaa
     3181 aatccaagat ggagatactg gagcgagtat ttatgatatg gtgggtgggg cttcttatct
     3241 gtgggaagct aaagaattag gaaaaagcac tattagccaa gaaaataatc caaaattttc
     3301 tcaaatgtca gatattgcta atcctagctt gcctaagcaa aacaaatata ccccatcgtt
     3361 agatcacaga aaacacatta cttttggaga atatgttgaa ggcggagttg gtaaacttac
     3421 tttgaaatca aatattgatc aaggtgcagg cggtttgtat tttgagggag attttatagt
     3481 ttcgcctacc caaaatgaaa catggaaagg ggcgggtata tctatcagtg atgaaagtac
     3541 cataatttgg aaagtcaatg gcgtggcaaa cgaccgcctg tccaaaatcg gcaaaggcac
     3601 gctgcacgtt caagccaaag gggaaaacca aggctcgatc agcgtgggcg acggtaaagt
     3661 tattttagat caacaagcag atgaaaataa taaaaaacaa gcctttagtg aaatcggctt
     3721 ggtcagcggc agggggacgg tgcaactgaa tgccgataat cagttcaacc ccgacaaact
     3781 ctatttcggc tttcgcggcg gacgtttgga tttgaacggg cattcgcttt cgttccaccg
     3841 tattcaaaat accgatgaag gggcgatgat tgtcaaccac aatcaagaca aagaatccac
     3901 cgttaccatt acaggcaata aagatattgc tacaaccggc aataacaaca gcttggatag
     3961 caaaaaagaa attgcctaca acggttggtt tggcgagaaa gatacgacca aaacgaacgg
     4021 gcggctcaac cttgtttacc agcccgccgc agaagaccgc accctgctgc tttccggcgg
     4081 aacaaattta aacggtaaca tcacgcaaac aaacggcaaa ctgtttttca gcggcagacc
     4141 gacaccgcac gcctacaatc atttaggaag cgggtggtca aaaatggaag gtatcccaca
     4201 aggagaaatc gtgtgggaca acgactggat caaccgcacg tttaaagcgg aaaatttcca
     4261 tattcagggc gggcaggcgg tgatttcccg caatgttgcc aaagtggaag gcgattggca
     4321 tttgagcaat cacgcccaag cagtttttgg tgtcgcaccg catcaaagcc acacaatctg
     4381 tacacgttcg gactggacgg gtctgacaaa ttgtgtcgaa aaaaccatta ccgacgataa
     4441 agtgattgct tcattgacta agaccgacat cagcggcaat gtcagccttg ccgatcacgc
     4501 tcatttaaat ctcacagggc ttgccacact caacggcaat cttagtgcaa atggcgatac
     4561 acgttataca gtcagccaca acgccaccca aaacggcgac cttagcctcg tgggcaatgc
     4621 ccaagcaaca tttaatcaag ccacattaaa cggaaacaca tcggcttcgg gcagtgcttc
     4681 atttaatcta agcaacaacg ccgtacaaaa cggcagtctg acgctttccg gcaacgctaa
     4741 ggcaaacgta agccattccg cactcaacgg caatgtctcc ctagccgata aggcagtatt
     4801 ccattttgaa aacagccgcc ttaccggaca aatcagcggc ggcaaggata cggcattaca
     4861 cttaaaagac agcgaatgga cgctgccgtc aggcacggaa ttaggcaatt taaaccttga
     4921 caacgccacc attacactca attccgccta tcgccacgat gctgcaggcg cgcaaaccgg
     4981 cagtgtgtca gacacgccgc gccgccgttc gcgccgttcg cgccgttccc tattatccgt
     5041 tacaccgcca acttcggtag aatcccgttt caacacgctg acggtaaacg gcaaattgaa
     5101 cggtcaggga acattccgct ttatgtcgga actcttcggc taccgaagcg acaaattgaa
     5161 gctggcggaa agttccgaag gcacttacac cttggctgtc aacaataccg gcaacgaacc
     5221 tgcaagcctc gaacaattga cggtagtgga aggaaaagac aacaaaccgc tgtccgaaaa
     5281 ccttaatttc accctgcaaa acgaacacgt cgatgccggc gcgtggcgtt accaactcat
     5341 ccgcaaagac ggcgagttcc gcctgcataa tccggtcaaa gaacaagagc tttccgacaa
     5401 actcggcaag gcagaagcca aaaaacaggc gggaaaagac aacgcgcaaa gccttgacgc
     5461 gctgattgcg gccgggcgcg atgccgtcga aaagacagaa agcgttgccg aaccggcccg
     5521 gcaggcaggc ggggaaaatg tcggcattat gcaggcggag gaagagaaaa aacgggtgca
     5581 ggcggataaa gacagcgcct tggcgaaaca gcgcgaagcg gaaacccggc cggctaccac
     5641 cgccttcccc cgcgcccgcc gcgcccgccg ggatttgccg caaccgcaac cccaaccgca
     5701 accccaaccg cagccccaac cgcagcccca accgcagcgc gacctgatca gccgttatgc
     5761 caatagcggt ttgagtgaat tttctgccac gctcaacagc gttttcgccg tacaggacga
     5821 attggaccgc gtgtttgccg aagaccgccg caacgccgtt tggacaagcg gcatccggga
     5881 caccaaacac taccgttcgc aagatttccg cgcctaccgc caacaaaccg acctgcgcca
     5941 aatcggtatg cagaaaaacc tcggcagcgg gcgcgtcggc atcctgtttt cgcacaaccg
     6001 gaccgaaaac accttcgacg acggcatcgg caactcggca cggcttgccc acggcgccgt
     6061 tttcgggcaa tacggcatcg gcaggttcga catcggcatc agcacgggcg cgggttttag
     6121 cagcggcagt ctttcagacg gcatcggaag caaaatccgc cgccgcgtgc tgcattacgg
     6181 cattcaggca cgataccgcg ccggtttcgg cggattcggc atcgaaccgc acatcggcgc
     6241 aacgcgctat ttcgtccaaa aagcggatta ccgctacgaa aacgtcaata tcgccacccc
     6301 cggccttgcg ttcaaccgct accgcgcggg cattaaggca gattattcat tcaaaccggc
     6361 gcaacacatt tccatcacgc cttatttgag cctgtcctat accgatgccg cttcgggcaa
     6421 agtccgaaca cgcgtcaata ccgccgtatt ggctcaggat ttcggcaaaa cccgcagtgc
     6481 ggaatggggc gtaaacgccg aaatcaaagg tttcacgctg tccctccacg ctgccgccgc
     6541 caaaggcccg caactggaag cgcaacacag cgcgggcatc aaattaggct accgctggta
     6601 acaccggata tgccgaaagg ggtttgacga tgccgccgtg cgctgtcaaa ccctttttct
     6661 gccgccgcct tgtgcctgct ccattgtctg ataaccgtca aattgatttt tagcccatgt
     6721 ttggtgggtc ggggaaatct atatcttcgt ccgtgccgaa atagtctatg cccgatatac
     6781 aattttgata cacaaacttg gaaatatcgg tatcgtcgcc ggagcgatag aatgcggaca
     6841 gtttttcatt gaattgcggc atctgcattt cggagatttc caagatgccg cagccccccg
     6901 ccatcatcag accgttggca aataaggttg ccgttcgttt gttgccgtcc caaaaaacct
     6961 gctgccgcat acaataaagc ataaaacgga cggctgcctc cgtcgtcgaa ccgctttgca
     7021 gtccgatatt ttgtaacacg cgggccactt caatttcctt cactggattc ggggcatgac
     7081 gggaaccgtc caatagcgtt acgccgaccg aaccggtacg gaaatcaccg ggtgccaaag
     7141 aatcgtcctt ggcaacaatg ttgttgattt ttttaaggag tgaaatatcg accggttcgc
     7201 cgtttgaaat atgcgaaatc acatattgat aggcacgttt caggttcaag atggtttgga
     7261 tgtcttccag tgacgcggag gctacatttt tgccatggat aatttgttcg gtctgcaata
     7321 aggtcgtctg acaattctca aaacggctga ggttgtgaat ttgggcaacc aatactttct
     7381 ttgccaaaaa aatattttct ttcaaactca acttgtattt gtcgggaaac acgataattc
     7441 cttaaataat taaagcagta cgttaaatca tcatcttatc atcactttcg gggtttatgt
     7501 tgcatgcgtc cgttttctaa tacggtttcc catcccgcga aacgaggcgg agacctgtgc
     7561 aaaaaacctg ttcacgaaat tttccgcatt aacgatacgc tgaaaatgcg ctaaaaatga
     7621 ctgtgtttga atatcggttg atttcatccg tttgtgtaac ctcgcgccgg ttttgtcatg
     7681 attttgcatt atagtgaatt aaatttaaac cagtacagcg ttgcctcgcc ttgccgtact
     7741 atctgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaattca ctatattttt
     7801 gtcatgcgga tatttcacgg gatgacaaaa cgggcgcaaa aaagcccgat tggaaaatcc
     7861 gaatcgggct tttttgtgat gttctgttcg atttgcgtac taactgccgg ccggagcgtc
     7921 cgcgcccggt ttggcaggtc ggattctcga atccgacggc tatttgagat ggcaggcttt
     7981 tgtcggatac aagtatccga cctacggctt gcttttcgtc attcccgcga aagcgggaat
     8041 ctagaatctc ggactttcag ataatctttg aatattgctg ttgttctaag gtctagattc
     8101 ccgcct
//
LOCUS       DAXHTY010000085         8036 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXHTY010000085 DAXHTY010000000
VERSION     DAXHTY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.085"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(78..494)
                     /gene="dksA"
                     /locus_tag="ACFNMK_001644"
     CDS             complement(78..494)
                     /gene="dksA"
                     /locus_tag="ACFNMK_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC6364870.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(641..1450)
                     /gene="proC"
                     /locus_tag="ACFNMK_001645"
     CDS             complement(641..1450)
                     /gene="proC"
                     /locus_tag="ACFNMK_001645"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC6364871.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLQKELGVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTL
                     SRYIGGTRRIVRVMPNTPGKIGLGVSGMFAEAEVSETDRRIADRIMKSVGLTIWLEDE
                     AQMHGITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVTLAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1481..1888)
                     /locus_tag="ACFNMK_001646"
     CDS             complement(1481..1888)
                     /locus_tag="ACFNMK_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC6364872.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQGGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1888..2574)
                     /locus_tag="ACFNMK_001647"
     CDS             complement(1888..2574)
                     /locus_tag="ACFNMK_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC6364873.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSEQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2694..3737
                     /gene="pilT"
                     /locus_tag="ACFNMK_001648"
     CDS             2694..3737
                     /gene="pilT"
                     /locus_tag="ACFNMK_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC6364874.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3900..5126
                     /locus_tag="ACFNMK_001649"
     CDS             3900..5126
                     /locus_tag="ACFNMK_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6364875.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5412..7562
                     /gene="yccS"
                     /locus_tag="ACFNMK_001650"
     CDS             5412..7562
                     /gene="yccS"
                     /locus_tag="ACFNMK_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC6364876.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIITTVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDNPDIRHLGRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAAIETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVATACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVVVSTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITEHLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1986 a         2651 c         1747 g         1652 t
ORIGIN      
        1 ccctcctctg actggtgcgc ctttgtgaat atgctgtctg aaactcgggg actcagacgg
       61 cattttgttt gccgccatca gtcggcaaac tgttttttca tcctctctcg gcgttcttgg
      121 gactcaacag ataaagtggc tgtcgggcgt gccagcagcc gcttcagacc gataggctct
      181 cccgtatcgg cacagaatcc ataatcccct tcatcaatat tgcggatggt cgcctgtatt
      241 ttactgagaa gttttcgttc ccgatcgcgg gtacggagtt ccaatgcgta ctcttcttcc
      301 tgtgtggcac ggtcggcagg atcgggggct gattcgtgtt cttggagatg ccctgtcgta
      361 gcggaagcat tttcgatgag ttcgtcttgc atttttacca gcaattcgcg gaaaaaagcc
      421 aaatgctcgt cattcatata atcgtcttcc ggcccgttcc aattcaaaat atcttgttct
      481 gtcagctttg ccataatgta tatcctctcg gttcaccgaa aaaaacaaag caccacaaag
      541 tggctttatt cctttgattt ttataaggct tattgcctaa ggcgtttttg aaaacgcgca
      601 tcataacacg ttttggattg gtttttttat tttctttaca ttattgatat tgccgttcca
      661 tttcctgcga acggcgcaca caggcacaaa cgccctcgct tatggcttcg gcgacacgat
      721 gccgcctgaa agcttccacg gcttcgtggg ttgtcccgcc ttttgacgtt acattttttt
      781 gaagcttctc gaaatcttca cccgtctgct cggcaagggt aaccgctcct ttaaacgttg
      841 ccagactgag cgcgcgtgct tctgccatat caaacccttg tcggatggcg gcattttgca
      901 atgcgtccag cagataaaac acataagccg gtccgctgcc gctgatgccg gtaatgccgt
      961 gcatttgcgc ttcatcttcc aaccaaatgg tcaaaccgac tgatttcatg attcgatcgg
     1021 caatcctgcg gtctgtttcc gatacttccg cttcggcaaa cataccggat acgcccagcc
     1081 cgatttttcc gggtgtattc ggcataaccc ggacaatgcg gcgtgttccc ccaatgtaac
     1141 ggctgagcgt atcgaccggc aatccggctg cgacagaaag caccaatgcg ccgttggtgc
     1201 ggatattttt gcacgcggct tccatatcct gcggcttgac ggcaaggatt aaaacatcgt
     1261 cggaatgaag ctccggcaag gtttcagaag tttcgacccc caactctttt tgcaaacgtt
     1321 cgcgcttttc cgcaccccgg ttggcaatat ggatgcggta accgccttgt ttgaccaatc
     1381 cgcccgcaac ggcagccgcc atattgccgc cgccgagaaa ataaacattc attatttcta
     1441 ccctattcaa aattacctta ctaaattcat tcccaatttt ttaccatcgg acgcgctcgg
     1501 acagaccgtt gcgccgcaac ccctgctttt ccaagcagtc aaatcctccc tgtttgaacg
     1561 tctcaatcaa aggtttgccg tcacgcgtac gccaatccag cccgcaggct tcaaacttcc
     1621 ctattttcct gtaaaagtaa gattgttgtg cggattcata ttcatttgcc ctgacggaac
     1681 gattgccgtc tgaaagcggt atctcgtagt cctctaaaga cggcggactc tcgccgccaa
     1741 tatcccaaaa atcgatggcg gcaggttttc tcggcttaca ccagccggac agggacgaac
     1801 actcatacca acctgccaac cggcagcctg caaccgtaaa tgcaagcaac gcagccattg
     1861 ccgtatattt tattgcccga atttccatca gccccttttc ccgaaaatcg cgctgccgat
     1921 acggacgtgt gtcgcaccgc actcaatggc ggcaggcata tcgtccgaca tccccataga
     1981 cagcacgtct gccttaacgc cagccgcatt gaggtcggca agcagtttcc gcatcgtttg
     2041 aaattgcacc ttcaactccg tttcactgct gttagctttg gcaacacaca tcagtccgcg
     2101 tacgacgata ttcggcagct tcgccacttc cacagcaagc gcgactgctt cttcgggcgc
     2161 gacaccgtgc ttcaccgcct cgcccgcaat gttcacctca atacacacct gcaaaggcgg
     2221 cattgaggaa ggacgctgtt cgctcaaccg gacagcggtt ttcagacggc atacggtatg
     2281 cacccaatgc gcgcgttcgg cgacaaactt ggttttgttg gactgcacat cgccgatgac
     2341 gtgccacacg atgtcggtca aatccgccaa ctcttccgtt ttgccgtacc actcctgaat
     2401 atagttctcg ccgaaatcac gctgtccggc ggcgtaaact tcgcggatgc cgtctgaagg
     2461 gaaagtctta ccgacggcaa tcaggctgac ggaatgcggc ttccttcccg cctgcagaac
     2521 caattttccg atacggtcgg acacctcaca ataacgttct tgcaacaccg tcatagatta
     2581 tcccctaatt aaaaatgatt aaacagttga aacccctcca gtcagggcgg tacaatcaag
     2641 gttgttagaa ccattccaac caatcgaaac attatactaa acagagccgc attatgcaga
     2701 ttaccgactt actcgccttc ggcgctaaaa acaaagcatc cgaccttcac ctgagttcgg
     2761 gcatatcccc tatgattcgg gttcacggcg atatgcggcg catcaacctt cccgaaatga
     2821 gcgcggaaga agtcggtaat atggtaactt cggtgatgaa cgaccaccag cggaaaatct
     2881 accagcaaaa cttggaagtc gacttctcgt tcgaactgcc caacgtcgcc cgattccgcg
     2941 tcaacgcctt caacaccggc cgcggtcccg ccgccgtatt ccgtaccatt cccagcaccg
     3001 tcttatcgct ggaagaattg aaagccccga gcattttcca aaaaatcgca gaatcgccgc
     3061 gcggcatggt attggttacc ggccctaccg gttcgggcaa atcgaccacg cttgccgcga
     3121 tgatcaacta catcaacgaa acccagccgg cacacatcct gaccatcgaa gacccgatcg
     3181 aattcgtcca ccaaagcaaa aaatccctga ttaaccagcg cgagctgcac cagcacaccc
     3241 tcagcttcgc caacgcgctg agttccgcat tgcgcgaaga ccccgacgtt atccttgtcg
     3301 gcgagatgcg cgacccagaa accatcggct tggctctgac cgccgccgaa accggacact
     3361 tggttttcgg cacgctgcac acgaccggcg cagcaaaaac cgtcgaccgt attgtggacg
     3421 tattcccggc gggagaaaaa gaaatggtgc gctctatgct gtccgaatcg ctgaccgccg
     3481 tcatctccca aaacctgctg aaaacgcacg acggcaacgg ccgtgtcgcc tcgcacgaaa
     3541 tcctgattgc caaccccgcc gtccgcaacc tcatccgcga aaacaaaatc acgcagatta
     3601 actccgtcct gcaaaccggg caggcgagcg gtatgcagac aatggaccaa tcgctgcaat
     3661 cgctggtgcg ccaagggctg atcgcaccgg aagtcgcacg cagacgcgcg caaaacagcg
     3721 aaagtatgag tttctgacac acaaccgcct tccggccata ccggcgggaa aacaaggcgc
     3781 aaacacgcgg ggcgggacgc agcatcccgc ccggctacct ttccgaacaa gaagcgtccg
     3841 ccttcctgtt gaaacctgcc gccgcaaact gcaaggctta aaccgaaaag aagctaacga
     3901 tgaataccga taacctgcac gacatcttgg acgaaatggt tcaagtgtat tctcaaaaaa
     3961 aacaaagccg atccgaaacc ccggccgaaa tcggcgcaca cttccacccg ctgctcgacc
     4021 gcctgtgcga aaccgcagaa gcacaaaacg cgtccgacat ccttatcagc aaaggattcc
     4081 cgccctcgtt gaaaatcaac agcgcattaa ccccgcagcc gcaaaaggcg ctgacgggcg
     4141 aggaaaccgc cgccatcgcc gcatcgacga tgaacgccga acaatcggaa atattccggc
     4201 gcgacggcga aatcaactac tccgtccagt cgcgcagcgg cacgcgctac cgcgccaacg
     4261 cctaccacag ccaaggcagc gcaggtttgg ttttgcggcg catcaaccac gtcatcccgc
     4321 aaatgcagga attgggtctg cccgaaaaac tcaaagacct cgccgtcgca ccgcgcgggc
     4381 tgctgattat cgtcgggcct accggttcgg gcaaatccac cacgatggcg actatgctcg
     4441 aacaccgcaa caaaaccctg cccagccata tcgttaccat cgaagacccg attgagttta
     4501 tctacaaacc gcgccgctgc atctttaccc agcgcgaaat cggcgtcgac accataaact
     4561 ggcagacggc ggtacaaaac gctatgcgcc agtcccccga cgtggtctgc atcggcgaag
     4621 tccgcagcag ggaaagtatg gaatacgcga tgcagctcgc ccaaaccggc cacctgtgca
     4681 tttttacgct ccacgccaac accgcgccgc agtcgctcga acgcatactc aacttctacc
     4741 ccaaagaaca gcacaaccaa atactgatcg acatcgccct caacctgacc ggcatcatct
     4801 gccaacgcct cgccctcaaa caagacaaaa cgggcaggac ggcggttgtc gacttgctca
     4861 tcaacacgcc cgccatccaa gacttcatcc tgaagggcga cctgatgaac atcagtaaaa
     4921 tcatggaaac cgccaaaacc gacggaatgc agacgatgga tcaaaacctt ttcgaactgt
     4981 accgtcacgg catcatcagt tacgaagaag ccctgcgcca gtccgtttcc gccaacaacc
     5041 tgcgattgca catccaactg cacaaagaag gcaaaacgcc cgaactcctt tacgacaggg
     5101 tcaacggtct caacctcatt tcctgatccg caaaacccaa tgccgtctga aaaccgcatc
     5161 cccgttttca gacggcatga ttttatccgc cccattcatg tgctacactt tatagtggat
     5221 taaatttata gtggattaac aaaaatcagg acaagacgac gaagccgcag acagtacaaa
     5281 tagtacggaa ccgattcact cggtgcttca gcaccttaga gaatcgttct ctttcagcta
     5341 aggcgaggca acgccgtact ggtttttgtt aatccactat attcacttca taacaataaa
     5401 ccggtaaaac catgaaaacc ccactcctca agcctctgct cattacctcg cttcccgttt
     5461 tcgccagtgt ttttaccgcc gcctccatcg tctggcagct aggcgaaccc aagctcgcca
     5521 tgcccttcgt actcggcatt attgccggcg gccttgtcga tttggacaac cgcctgaccg
     5581 gacggctgaa aaacatcatc accaccgtcg ccctgttcac cctctcctcg ctcacggcac
     5641 aaagcaccct cggcacaggg ctgcccttca tcctcgccat gaccctgatg accttcggct
     5701 ttaccatttt aggcgcggtc gggctgaaat accgcacctt cgccttcggc gcactcgccg
     5761 tcgccaccta caccacactt acctacaccc ccgaaaccta ctggctgacc aaccccttca
     5821 tgattttatg cggcaccgta ctgtacagca ccgccatcat cctgttccaa atcatcctgc
     5881 cccaccgccc cgtccaagaa agcgtcgcca acgcctacga cgcactcggt ggctacctcg
     5941 aagccaaagc cgacttcttc gaccccgatg aggcagcctg gataggcaac cgccacatcg
     6001 acctcgccat gagcaacacc ggcgtcatca ccgccttcaa ccaatgccgt tccgccctgt
     6061 tttaccgcct tcgcggcaaa caccgccacc cgcgcaccgc caaaatgctg cgctactact
     6121 tcgccgccca agacatacac gaacgcatca gctccgccca cgtcgactac caagagatgt
     6181 ccgaaaaatt caaaaacacc gacatcatct tccgcatcca ccgcctgctc gaaatgcagg
     6241 gacaagcctg ccgcaacacc gcccaagccc tgcgcgcaag caaagactac gtttacagca
     6301 aacgcctcgg ccgcgccatc gaaggctgcc gccagtcgct gcgcctcctt tcagacggca
     6361 acgacaatcc cgacatccgc cacctcggcc gcctgctcga caacctcggc agcgtcgacc
     6421 aacaattccg ccaactccag cacaacggcc tgcaggcgga aaacgaccgc atgggcgaca
     6481 cccgcatcgc cgccatagag accggcagcc tcaaaaacac ctggcaggca atccgtcctc
     6541 agctaaacct cgaatcaggc gtattccgcc atgccgtccg cctgtccctc gtcgttgcca
     6601 ccgcctgcac catagtcgaa gccctcaacc tcaacctcgg ctactggata ctgctgaccg
     6661 cccttttcgt ctgccagccc aactacaccg ccaccaaaag ccgcgtccgc cagcgcatcg
     6721 ccggtaccgt actcggcgta atcgtcggct cgctcgtccc ctacttcacc ccgtctgtcg
     6781 aaaccaagct ttggatcgtc gtcgtcagca ccaccctgtt cttcatgacc cgcacctaca
     6841 aatacagttt ctccaccttc ttcattacca ttcaagccct gaccagcctc tccctcgcag
     6901 gtttggacgt atacgccgcc atgcccgtac gcatcatcga caccattatc ggcgcatccc
     6961 ttgcctgggc ggcagtcagc tacctgtggc cagactggaa atacctcacg ctcgaacgca
     7021 ccgccgccct tgccgtatgc agcaacggtg cctatctcga aaaaatcacc gaacacctca
     7081 aaagcggcga aaccggcgac gacgtcgaat accgcgccac ccgccgccgc gcccacgaac
     7141 acaccgccgc cctcagcagc accctttccg acatgagcag cgaacccgca aaattcgccg
     7201 acagcctgca acccggcttt accctgctca aaaccggcta cgccctgacc ggctacatct
     7261 ccgccctcgg cgcataccgc agcgaaatgc acgaagaatg cagccccgac tttaccgcac
     7321 agttccacct cgccgccgaa cacaccgccc acatcttcca acacctgccc gaaaccgaac
     7381 ccgacgactt tcagacggca ttggatacac tgcgtggcga actcgacacc ctccgcaccc
     7441 acagtagcgg aacacaaagc cacatcctcc tccaacagct ccaactcatc gcccgacagc
     7501 tcgaacccta ctaccgcgcc taccgccaaa ttccgcacag gcagccccaa aacgccgcct
     7561 gaaaaagttt cggcattttg taagagagag cagattgtca gacaggttgc aagatagtgg
     7621 ataaagcttt gcgcagggta aatgcgtagc aactgaaccg tcattcccac gaatctacat
     7681 tccgtcattc ccacgaaaac agaaaaccaa aaacagaaac ctaaaatccc gtcattccca
     7741 cgaacctaca ttccgtcatt cccacgaaaa cagaaaacca aaaacagaaa cctaaaatcc
     7801 cgtcattccc acgaacctgc atcccgtcat tcccacgaaa gtgggaatcc agaatctcta
     7861 aagcttcagc taacctttga atattgctgt tgttctaagg tctagattcc cgcctgcgcg
     7921 ggaatgacgg catcggtttg acggtattta attgaattgt ggaaattgat ggattcagtg
     7981 agattggcga gatgaagcct accctatagc ccgcctttta cgaacccgcc ccctcc
//
LOCUS       DAXHTY010000086         8035 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXHTY010000086 DAXHTY010000000
VERSION     DAXHTY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.086"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(210..1595)
                     /locus_tag="ACFNMK_001651"
     CDS             complement(210..1595)
                     /locus_tag="ACFNMK_001651"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC6364877.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLVGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGIVLKEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(1694..4084)
                     /gene="gyrB"
                     /locus_tag="ACFNMK_001652"
     CDS             complement(1694..4084)
                     /gene="gyrB"
                     /locus_tag="ACFNMK_001652"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC6364878.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSSEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            4354..5226
                     /locus_tag="ACFNMK_001653"
     CDS             4354..5226
                     /locus_tag="ACFNMK_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC6364879.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAVVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            5314..7350
                     /locus_tag="ACFNMK_001654"
     CDS             5314..7350
                     /locus_tag="ACFNMK_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC6364880.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDGGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSTHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAV"
BASE COUNT         1727 a         2254 c         2219 g         1835 t
ORIGIN      
        1 tcttgccgga tgctgcaaac aacgccggtt tgcattcctg atggcggtgg ttttcttaga
       61 cgaacgcccg aacacgcagg aatggatagg cttggggctg gttacggcgg gcgtgttgac
      121 gctggcactg aaacggtaaa gccgcaagaa ataaatgaaa tgccgtctaa aaaactgttt
      181 tcagacggca ttttcgtttc tgtccatcct cagcactcga ccacgcgcac ggatacgggg
      241 acggcttttt tccggagcgt ggcggcgagt ccggcaagcg aggtttcttt gtaggtcgcc
      301 ttcatatccc ggccggtttg cagcatggtt cggatgactt cgtcgagcga gacttttttg
      361 tccgtgccgt cttccaaaag cgcgagcgtg ccgagtttga gggctttttc ggcggcgatg
      421 ccgttgcgct cgatgcaggg gatttgcacc agtccgccga cggggtcgca agtcagcccc
      481 aaatggtgtt ccatcgccat ttcggcggcg ttttccactt gtttgggcgt gccgccgatg
      541 acttcggcgt atgcgcccgc cgccatcgaa cacgctacgc cgacttcgcc ctgacagccg
      601 acatccgcac cggaaatgga ggcgttggtc ttgtagagga tgccgattgc gcctgcggtg
      661 agcaggaagt tttcgacgcg ttcctgtgtg gcgtgcggat tgaacttgcg gaaatagtgc
      721 aatacggcgg gaatgatgcc tgccgcgccg ttggtcggtg cggtaacgac gcgtccgccg
      781 gcggcgtttt cttcgttgac cgccatggcg tacaccatcg gccagagctg ggtgttgacg
      841 atttcggttt cgcgcaggac tttgagcttg gcggcaagct gcggggcgcg gcggcggacg
      901 ttcaatccgc cgggcagttc gccgtccgca cccaagccgc gtttgatgca gccttccata
      961 acctcggcaa cggcagcggc gcggcggcgg atttcggctt cgccgcatcc ggcaagcgcg
     1021 gcttcgtttg ccaacacgac ttcggagatg tcgagccggt tcagacggca tcgggcaagc
     1081 agttcggcgc aactggtata gggataggga acggcttttt ccgtttccgc ctgccggtca
     1141 aaatcttctt cggtaacgac aaagccgccg ccgaccgaat aataaacctg ttctttcaag
     1201 acgatgccgt ctgaagcata ggcggtaaaa cgcaggccgt tggggtgttt gggcagcact
     1261 tgattgccga gtatgttcag gtcgcggtcg gggatgaagc ggatttcttg cccgttgagc
     1321 cggaggatgt gctgcgtgcg gatgcgttcg aggcgttcgg gaatgccgac aagcgggatg
     1381 tcgtgcggca ggctgccttc caaaccgagc atcagtgcgt caaatgtacc gtgtccgtat
     1441 ccggtcaggg cgagcgagcc gtaaatgtcg atgacgatgc gaacagcctg tgcatccaaa
     1501 cctgccgcaa aggcggcggc tgccttcatc gggccgaccg tatgcgaact ggaaggcccg
     1561 ataccgattt tgaaaatatc aaaaatgctg accatggttt tgctccgaaa ggcggtttca
     1621 gacggtatgg cttccgtttg acaaaccaaa aggagacgcg gcacgatgcc cgtctccttt
     1681 tttaaaacgg cacttatgcg tcgatatttt gggcaatcag cgcgttgttt tcgataaagg
     1741 cgcggcgcgg ttcgacctcg tcgcccatca gggtaacgaa tacttcgtcg gcggcaatgg
     1801 cgtcttcgat gcgcactttc aacagacggc gcacggcggg atccatcgtg gtttcccaca
     1861 gctgctcggg gttcatctcg cccaagcctt tgtatcgttg gatggacata cctttttggg
     1921 caacgctcat caagatgtcc aaagcggttt caaagctgtc cgcgtcgtac ccgttttcgc
     1981 ctttgtaaag cttggcaccc tcgccgacca tgcctttgag cgcggcggcg gtttgggtga
     2041 gggtttggta ggctttgctg ttgaggaact tgggttcgat gtagctgacc atgacgttgc
     2101 cgtgcagctt gcgcgtgatt ttgatgaacc ggtgtccttc atgaccttcg atgcgttcga
     2161 gggcgacttc tttttcgtca agcagaccgg aaagttcggc aacggcttta tcggcgtttt
     2221 cagacgacgt caaatcaatg ggcgacgcgt gtagcatggc gcgcaggacg agttcgtcta
     2281 cgaagcggct ttcctgttcg atgacggttt ttgccaacag gaattgtttg gcggtgtcgg
     2341 caagttctgc gccttcgatg gtgcggccgt ctgaaacgat tttggctttt tccaaggcaa
     2401 gaccgagcag ccattggtct ttttccaact cgtccttgag gtaacgttcc tgtttgccgt
     2461 atttcgcttt atacaaaggc ggctgggcga tatagatgta gccgcgctcg accagttcgg
     2521 gcatttggcg gtagaagaag gtcaggagca gggtgcggat gtgcgcgccg tccacgtcgg
     2581 catcggtcat gatgatgatg cggtggtagc gcagcttctc ggcattgaat tcttctttgc
     2641 cgatgcccgc gcccaacgcg gtaatcaaag cggctacttc ctgactggcg agcatttttt
     2701 caaagcgcgc tttttcgacg ttcaaaatct tgcccttaag cggcaaaatc gcttggaatt
     2761 tgcggtcgcg cccctgcatg gcggaaccgc ctgcagaatc gccctcgacc agataaagtt
     2821 ctgacaatgc ggggtctttt tcttggcagt cggcgagttt accgggtagc cccaagccgt
     2881 ccattacgcc tttgcggcgg gtgatttcgc gggctttgcg ggcggcttcg cgcgcgcggg
     2941 cggcatcgac gattttgccg gtgatgattt tggcttcgtt cgggttttct tcaaggaagt
     3001 cggtcagcgc ttggttgatg acttcgttga caacggggcc gatttcgctg gaaaccagtt
     3061 tgtctttggt ttgggatgag aatttggggt cgggcagttt gacggacaac acgcaggtca
     3121 aaccttcgcg catatcgtcg cctgctgttt ccactttggc ttttttggcg acttcgttgg
     3181 cttcgatgta gctgttgatg gtgcgtgtca tcacttggcg caacgcggtc aagtgcgtac
     3241 cgccgtcgcg ctgagggatg ttgttggtga agcactgcac gctttcttgg tagctgtcgt
     3301 tccactgcat cgcgcattcg acgctcatgc cgtctttctc gccgaacgca tagaagattt
     3361 tttcgtgcaa cggcgttttt ttgcggttca tgtattgcac gaaaccggcc acgccgccgg
     3421 aaagggcgaa gctttcgtgt ttgccgtcgc gttcgtcggt caattcgatg tccacgccgt
     3481 tgttcaggaa ggaaagttcg cgaatgcgtt tggcgaggat gtcgaagctg tattcgacgt
     3541 tgccgaaagt ttccgtgctg gcgaggaagc gtacggtcgt gcctttcttg tcggaatcgc
     3601 cgacaacttt cagcggctct tcggtttcgc cgcgcacgaa gcggacgaag tgttctttgc
     3661 cgtcgcggta gatggtcagc gtaacccagt cggacagcgc gttgacgacg gacacgccca
     3721 cgccgtgcag gccgccggag attttgtagc tgttgttgtc gaatttaccg cccgcgtgca
     3781 agacggtcat gatgacttcg gcggcggagc gtccttcttt cgggtggatg ccggtgggca
     3841 taccgcgccc attgtcggcg acgctgacgg aatggtcggc gtggatggta acggtgattt
     3901 tgtcgcaatg tccggcgagt gcttcgtcaa tggcgttgtc caatacttcg aacaccatgt
     3961 ggtgcaggcc gctgccgtcc tgcgtgtcgc cgatgtacat accggggcgt ttgcgtaccg
     4021 cttccaagcc ttcgagcacc tgaatgctgt cggcgccgta ttcttcgtgt ttttgttcag
     4081 tcatattttt tgccggattt tgaaaagatt ttgcgatgcc gccaaaacaa gtccgcacct
     4141 tgtggaaaaa gcgggcggga cgacatatat aattgtgtat tatagccgat tttgccgcct
     4201 aattcagcgt tatccgcatc agtgtgccgc cgggaaaaga tgaaacggta cgtttgcctc
     4261 cggcatcagg tcggggattg tcccgtaaag tggcaaaagc gtttttttgc cactaaaatc
     4321 tacaccctat acttttcgga caggggcgcg gaaatggaaa tatggaatat gttggacact
     4381 tggctcggtg ccgtcccgat acgtgcggag gcggtcgaat ccgtggcggt ggtcgcggct
     4441 ttgctgctgg cgcgcgccct tctgttgaat atccacttca aacggcatcc ggatttcggc
     4501 atcgaaagca agcggcggtt tttggttgcc agccgcaata taacgctgct tttggtgctg
     4561 ttttcgctgg catttatctg gtcggcgcaa atccaaacgc tggctttgtc gatgtttgcg
     4621 gtggcggcgg cggtcgtcgt ggcgacgaag gaactgatta tgtgtctgtc gggcagtatt
     4681 ttaaggtctg ccacccagca atactcggtc ggcgactata tcgaaatcaa cggcctgcgc
     4741 gggcgcgtgg tcgacatcaa cctgttgaac acgctgatga tgcaggtcgg tccgaacccc
     4801 ttggtcggac agcttgcggg aaccaccgtt tctttcccca acagcctgtt gttgagccac
     4861 cccgtgcgcc gcgacaatat tttgggcgac tatgtcatcc atacggtcga aatccccgtt
     4921 cccatccatt tggattcgga tgaagccgta tgccgtctga aagccgtact cgagcccttg
     4981 tgcgcgccct acatccccgc catccaacgg catttggaaa acgtgcaggc ggaaaaactg
     5041 tttatcacgc ccgccgccag accgcgcgtt acccgcgtgc cgtacgatga caaggcatac
     5101 cgcatcatcg tccgcttcgc ctcccccgtt tcaaagcggc tggaaatcca acaggcggtt
     5161 atggacgaat ttttgcgcgt acaataccgc ctgttaaatt accccgccgg ctccgaaaca
     5221 ctttaacttt ccccgaccga ccccatttcc ggcttcagac ggcatattgc cgatatgctg
     5281 tctgaagcac aacacgcaaa ggaaacccat cttatgactg acaacgcact gctccatttg
     5341 ggcgaagaac cccgttttga tcaaatcaaa accgaagaca tcaaacccgc cctgcaaacc
     5401 gccatcgccg aagcgcgcga acaaatcgcc gccatcaaag cccaaacgca caccggctgg
     5461 gcaaacactg tcgaacccct gaccggcatc accgaacgcg tcggcaggat ttggggcgtg
     5521 gtgtcgcacc tcaactccgt cgccgacacg cccgaactgc gcgccgtcta taacgaactg
     5581 atgcccgaaa tcaccgtctt cttcaccgaa atcggacaag acatcgagct gtacaaccgc
     5641 ttcaaaacca tcaaaaactc ccccgagttc gacaccctct cccccgcgca aaaaaccaaa
     5701 ctcaaccacg atctgcgcga tttcgtcctc agcggcgcgg aactgccgcc cgaacagcag
     5761 gcagaactgg caaaactgca aaccgaaggc gcgcaacttt ccgccaaatt ctcccaaaac
     5821 gtcctagacg cgaccgacgc gttcggcatt tactttgacg atgctgcacc gcttgccggc
     5881 attcccgaag acgcgctcgc catgtttgcc gctgccgcgc aaagcgaagg caaaacaggc
     5941 tacaaaatcg gtttgcagat tccgcactac ctcgccgtca tccaatacgc cgacaaccgc
     6001 gaactgcgcg aacaaatcta ccgcgcctac gttacccgcg ccagcgaact ttcagacggc
     6061 ggcaaattcg acaacaccgc caacatcgac cgcacgctcg caaacgccct gcaaaccgcc
     6121 aaactgctcg gcttcaaaaa ctacgccgaa ttgtcgctgg caaccaaaat ggcggacacg
     6181 cccgaacaag ttttaaactt cctgcacgac ctcgcccgcc gcgccaaacc ctacgccgaa
     6241 aaagacctcg ccgaagtcaa agccttcgcc cgcgaaagcc tgaacctcgc cgatttgcaa
     6301 ccgtgggact tgggctacgc cagcgaaaaa ctgcgcgaag ccaaatacgc gttcagcgaa
     6361 accgaagtca aaaaatactt ccccgtcggc aaagtattaa acggactgtt cgcccaaatc
     6421 aaaaaactct acggcatcgg atttaccgaa aaaaccgtcc ccgtctggca caaagacgtg
     6481 cgctattttg aattgcaaca aaacggcgaa accataggcg gcgtctatat ggacctctac
     6541 gcccgcgaag gcaaacgcgg cggcgcgtgg atgaacgact acaaaggtcg ccgccgtttt
     6601 tcagacggca cgctgcaact gcccaccgcc tacctcgtct gcaacttcgc cccacccgtc
     6661 ggcggcaggg aagcccgcct gagccacgac gaaatcctca tcctcttcca cgaaaccgga
     6721 cacgggctgc accacctgct tacccaagtg gacgaactgg gcgtgtccgg tatcaacggc
     6781 gtagaatggg acgcggtcga actgcccagc cagtttatgg aaaatttcgt ttgggaatac
     6841 aatgtattgg cgcaaatgtc cacccacgaa gaaaccggcg agcccctgcc gaaagaactc
     6901 ttcgacaaaa tgctcgccgc caaaaacttc caacgcggaa tgttcctcgt ccgccagatg
     6961 gagttcgccc tcttcgatat gatgatttac agcgaagacg acgaaggccg tctgaaaaac
     7021 tggcaacagg ttttagacag cgtgcgcaaa gaagtcgccg tcatccaacc gcccgaatac
     7081 aaccgcttcg ccttgagctt cggccacatc ttcgcaggcg gctattccgc aggctattac
     7141 agctacgcgt gggcggaagt attgagcgcg gacgcatacg ccgcctttga agaaagcgac
     7201 gatgtcgccg ccacaggcaa acgcttttgg caggaaatcc tcgccgtcgg cggatcgcgc
     7261 agcgcggcag aatccttcaa agccttccgc ggccgcgaac cgagcataga cgcactcttg
     7321 cgccacagcg gtttcgacaa cgcggtctga cggcagggtt gaagtaaaaa atatggcgga
     7381 ttcgatagaa aaacatccgc accgtcattc ccgcgcaggc gggaatccag accggtcggt
     7441 gcagaaactt atcgggaaaa acggtttctt tagattttac gttctagatt cccactttcg
     7501 tgggaatgac gcggaaaagt tgctgtgatt tcggataaat tttcgcaacg tttaatttcc
     7561 gttttacctg ataaatgccc gcaatctcaa atcccgtcat tccccaaaaa caaaaaaatc
     7621 aaaaacagaa atcccgtcat tcccgcgcag gcgggaatcc agaccggtcg gcacggaaac
     7681 ttatcggata aaacggtttc tttagatttt acgttctaga ttcccgcttt cgcgggaatg
     7741 acgcggaaaa gttgctgtga tttcggataa attttcgcaa cgtttaattt ccgttttacc
     7801 tgataaatgc ccgcaatctc aaatcccgtc attccccaaa aacaaaaaaa atcaaaaaca
     7861 gaaatcccgt cattcccgcg caggcgggaa tccaggtctg tcggtgcgga aacttatcgg
     7921 ataaaacggt ttctttagat tttacgttct agattcccgc tttcgcggga atgacggaat
     7981 atttttgaat ttgataaaaa tgccgtctga aacggtcaaa caacgcttca gacgg
//
LOCUS       DAXHTY010000087         7679 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXHTY010000087 DAXHTY010000000
VERSION     DAXHTY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.087"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(92..176)
                     /locus_tag="ACFNMK_001655"
     tRNA            complement(92..176)
                     /locus_tag="ACFNMK_001655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:Leu,seq:caa)
     gene            453..1106
                     /locus_tag="ACFNMK_001656"
     CDS             453..1106
                     /locus_tag="ACFNMK_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC6364881.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDSERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1185..1838
                     /gene="hisG"
                     /locus_tag="ACFNMK_001657"
     CDS             1185..1838
                     /gene="hisG"
                     /locus_tag="ACFNMK_001657"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC6364882.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSYLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1942..2841
                     /locus_tag="ACFNMK_001658"
     CDS             1942..2841
                     /locus_tag="ACFNMK_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC6364883.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2838..4127
                     /gene="hisD"
                     /locus_tag="ACFNMK_001659"
     CDS             2838..4127
                     /gene="hisD"
                     /locus_tag="ACFNMK_001659"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC6364884.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLKAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4173..5270
                     /gene="hisC"
                     /locus_tag="ACFNMK_001660"
     CDS             4173..5270
                     /gene="hisC"
                     /locus_tag="ACFNMK_001660"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC6364885.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5267..6184
                     /gene="hisB"
                     /locus_tag="ACFNMK_001661"
     CDS             5267..6184
                     /gene="hisB"
                     /locus_tag="ACFNMK_001661"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC6364886.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6279..7148
                     /locus_tag="ACFNMK_001662"
     CDS             6279..7148
                     /locus_tag="ACFNMK_001662"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC6364887.1"
                     /translation="MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            7273..7635
                     /locus_tag="ACFNMK_001663"
     CDS             7273..7635
                     /locus_tag="ACFNMK_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFC6364888.1"
                     /translation="MNRLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKETFAKSPKIP"
BASE COUNT         2031 a         2243 c         1820 g         1585 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatattt agttttatct atttcattaa acagcaatag
       61 acaaaaaaaa taaccgctct aaaagcggtt gtggtgccca gggtcggact cgaaccgaca
      121 caccttgcgg cgggggattt tgagtcccct gcgtctacca atttcgccac ctgggctggt
      181 gaagaagtcg tcattataat ggcttttgaa attctgtaaa cctttttttt gaaattattt
      241 tatctgtttt tattttattt ttgattttaa atagaatttt tattatttta atcttactgt
      301 tctttccgct ccaaagattc tgtatgattc ggcaattcct gccgtgcaga caacgtaaaa
      361 aaatactaca ttaaatctgc caaacgcgtt aagatggaaa tattcaaatt ccgtacgaat
      421 caggttttgc tatttattct tgggagattg tcatgttttc cgtaccgcgt tcctttttgc
      481 cgggcgtttt cgtacttgcc gcgcttgccg cctgcaaacc tcaagacaac agtgcggcgc
      541 aagtcgcttc ttcaagtgca tccgcgtcgg ctgcggaaaa tgcggcaaag ccgcaaacgc
      601 gcggtacgga tatgcgtaag gaagacatcg gcggcgattt cacgctgacc gacggcgaag
      661 gcaagccttt caacctgagc gatttgaaag gcaaggtcgt gattctgtct ttcggcttta
      721 cgcactgtcc cgatgtctgc ccgacagagc ttttgacgta cagcgacacg ttgaagcagt
      781 tgggcgggca ggctaaggac gtgaaagtgg tgttcgtcag catcgattcg gaacgcgaca
      841 cgcctgaaat catcggcaag tatgccaaac agttcaatcc ggactttatc ggtctgacgg
      901 caacgggcgg ccaaaacctg ccggtcatca agcagcaata ccgcgtggtt tctgccaaag
      961 tcaatcaaaa agacgacagc gaaaactatt tggtcgacca ctcttccggt gcgtatctca
     1021 tcgacaaaaa cggtgaggtt gccattttct cgccttacgg aagcgagccg gaaacgattg
     1081 ctgccgatgt aaggaccctg ctctgataaa accgtatgcc gtctgcaccg tcggcgccta
     1141 ttcagacggc attattgttt caaccgacaa aggacatcca caccatgcag gataatgctt
     1201 tgaccatcgc cttatccaag gggcgcattt ttgaggagac gctgccgctg cttgccgctg
     1261 ccggcattgt tccgactgaa gagcctgaaa aatcgcgcaa gctgattatc gggacgaacc
     1321 atgaaaacat ccgccttgtc attgtccgcg caaccgatgt gccgacttat gtccgctacg
     1381 gcgcggcgga cttcggcatt gcgggcaaag acgtgctgat cgaacacggc ggcacggggc
     1441 tttaccggcc tttggatttg gagattgcca agtgccgcat gatggttgct gtgcgtaaag
     1501 ggtttgatta cgaagcagct tcgcaacccg gatgccgtct gaagattgcc acaaagtatc
     1561 ctgaaatcgc ggcatctcat tttgctggca agggtgtcca tgtggacatt atcaaactgt
     1621 acggctcgat ggaacttgcg ccgctggtcg gcttgagcga tgcgattgtg gacttggttt
     1681 cgacgggcaa caccttgaag gcaaacggct tggaagcagt cgaacacatc gtcgacattt
     1741 ccagctacct ggtggtcaac aaggctgctt tgaaaacgaa atacgcgctg ctggagccga
     1801 ttattcaggc gttcggcggc gcagtgaagg cgaagtaagc atccatttga ataaagatgc
     1861 gttttcagac gaccctatcc gttcccgccg acaggtcgtc tgaaaatatc accggcagta
     1921 aactgtatag gagaagttaa aatggttgca aaaataaaaa aattctcaga ttcaaccctt
     1981 tccgttttga ataacggcga gcgtcggttt tatgtctatt gtctgaccga cctgaaaaaa
     2041 gacaaaatcc tctacatcgg caaaggctgc ggtaatcgta tcttcgagca tgaatgggtt
     2101 gctagtcgtt cacaagatcc agtctccggc gagattatcg atcggaaact caaagccatc
     2161 tccaaatgca agaaactcgg tcgctatatc atcagctatc atctgactga agtcgaagca
     2221 ctcgccgccg aatctgcctt aattcatttt gttaaatctg tcttgggtaa aaaactcaaa
     2281 aataaaattg ccgggcatgg tccgggtggt attagcgtag aagaactaga tcgccgcttt
     2341 ggattctctt ctctcccact taacgagatt aaccccgacg ggctgattct cgccatcaaa
     2401 atccacaatg ctttcgattt agatactgac gaagaattag actacctttt cgacaaccaa
     2461 gacgatgcca acctcaaatc gcgtacgttg ggcaactggg ttatcggtaa agatgttgct
     2521 tcaaaagtga aatacgttat cggcgttcac accggtctgc aaaacgctgt tgtcagtgca
     2581 tacgaagtgg acggttttga aacaatggtt gaggaaacca aaaacggtag aaaacaatcc
     2641 cgttaccgtt tccgcactac ctctcgtagc gaagaggtat tagccaaact cggtctgcaa
     2701 caaaaatgcc tgcccgaatt gaagtttggt agcgggggag aaaaagcgta tatcagaccc
     2761 aaaacagaga cagaaactga acaagagaat attcagacga cccccaatcc aaaaataaaa
     2821 aaggaaaaaa ccaaatcatg aaaaaactca acacccaatc gcccgatttc caagccggac
     2881 tcaaagccct gctggctttt gaaaccgcgc aaaaccccga aaccgaacgc atcgtcgccg
     2941 acatttgcgc cgacgtgcaa aagcgcggcg atgcggcttt gattgaatac accaacaaat
     3001 tcgatcagac aaacgctaaa agcatcgatg atttaatact cacgcaagcc gatttgaaag
     3061 cggcgttcga gcgcattccg aacgacgttc agacggcatt gcagaccgcc gcccgccgtg
     3121 tcgaaagcta ccaccaacgc caaaaaatgg aatcgtggag ctacaccgat gaagacggca
     3181 cgctgttggg acaacaaatc acaccgcttg accgcgtcgg catttacgtc cccggcggca
     3241 aggcggcgta tccgagttcc gtcatcatga acgccatgcc cgcccacgtc gcaggtgtga
     3301 aagaaatcat catggtcgtg ccgacaccaa aaggcgaacg caacgacatc gtacttgccg
     3361 ccgcatacgt cgccggcgta accaaagtct tcaccgtcgg cggtgcgcag gcggttgccg
     3421 ccctcgccta cggcacggaa accatccccc aagtcgataa aatcaccggt ccgggcaacg
     3481 ccttcgtcgc cgccgccaaa cgccgcgtgt tcggcgtggt cggcatcgac atggtggcgg
     3541 ggccgtctga aatcctggtc atcgccgacg gcacgacacc tgccgattgg gtggcgatgg
     3601 atttgttcag ccaggccgaa cacgacgaaa ttgcccaagc catcctcatc ggcacgtcgc
     3661 aagcgtatct cgacgaagta gaagccgcta tggacaggct catcgaaacc atgccgcgcc
     3721 gcgacatcat cgaagcctcg ctcggcaaca ggggcgcgat gatactcgcc aaagacttgg
     3781 acgaagcctg cgaaatcgcc aactacattt cccccgaaca cttggaactg tcagtcgaaa
     3841 atccgcagga atgggcgaaa aaaatccgcc acgccggtgc gattttcatg ggacgctaca
     3901 ccggcgaaag cctcggcgac tactgcgccg gtccaaacca tgtattgccc accagccgaa
     3961 ccgcccgctt ttcctcgcct ttggggacat atgatttcca aaaacgctcc agcctgattc
     4021 aggtttcgga acagggcgcg caaaaattag gcgaaaccgc cagcgtgctg gcacacggcg
     4081 aaagcctgac cgcccacgcc cgcgcggcag agttccgtat gaaataatgc cgaaacggcg
     4141 tacaggcata ttccaaccat taaggaaaca cgatgaaatc cgtccgctcc ttcatccgcg
     4201 acgacataca agctatgtcg gcatatcaga ttgccgacgt tccgcccggc tttgccaaac
     4261 tcgattcgat ggaaagtccc gtccaccctt ttgccggaca tgaaacgctg ttgcaggaat
     4321 ggcaggcacg gcttgccgcc gcgcccatcc atctttaccc caatccctcc ggcagcggtt
     4381 tacaggaagc attacgttcg gcgttcgaca ttcccgactg cgccgacatc gcgctgggca
     4441 acggctcgga cgagctgata cagttcatca cgatgctgac cgccaaaccg ggcgcggcaa
     4501 tgttggcagc cgaacccagt ttcgtcatgt accgccacaa cgccgcgctg tacggcatgg
     4561 attatgtcgg cgttccactg aacggagatt tcaccctcaa cctgcccgcc gtcctcgaag
     4621 ccgtcaggaa gcaccgccct gccctgacct ttatcgccta ccccaacaac cccaccggcg
     4681 tatgtttcac gcgtgccgaa atcgaagccg tcatcgaagc ttcagacggc atcgtcgtcg
     4741 tcgacgaagc ctacggcgca ttcaacggcg acagcttcct gccgcaggcg ggcagcattc
     4801 ccaacctcat cgtcatgcgt accgtcagca aaatcggttt tgccggactg cgtatcggct
     4861 atgcggcagg ctgtcccgaa gtcatcggcg aactgcaaaa aatcctgccg ccctacaata
     4921 tgaaccaact gagcctgacc accgccaaac tcgccctgca acactacggc atcatctctg
     4981 ccaacatcga cagcctgaaa aacgaacgcg aacggatgtt cgccgaattg ggcaaaatat
     5041 gccgtctgaa cacctttcca agtcaggcaa acttcattac catacgcgta cccgatgccg
     5101 atttgttgtt tgacacgctc aaacaaaacc gcatcttggt taaaaaactg catggcgcgc
     5161 acccgctttt ggaacactgc ctgcgcatta ccgtaggcag tcccgcacaa aacgatgccg
     5221 ttctcaacat cattcgccaa ctttactgcc aaccaacgga tttcctatga atttgactaa
     5281 aacacaacgc caactgcaca actttctgac cctcgcccaa gaagcaggtt cactgtccaa
     5341 gctcgccaaa ctctgcggct accgtacccc tgtcgcactc tacaaactca aacaacgcct
     5401 tgaaaagcag gcagaagacc cagatgcacg cggcatccgt cccagcctga tggcaaaact
     5461 cgaaaaacac accggcaaac ccaaaggctg gctcgacaga aaacaccgcg aacgcactgt
     5521 ccccgaaacc gccgcagaaa gcaccggaac tgccgaaacc caaattgccg aaaccgcatc
     5581 tgctgccggc tgccgcagcg ttaccgtcaa ccgcaatacc tgcgaaaccc aaatcaccgt
     5641 ctccatcaac ctcgacggca gcggcaaaag caggctggat accggcgtac ccttcctcga
     5701 acacatgatc gatcaaatcg cccgccacgg catgattgac atcgacatca gctgcaaagg
     5761 cgacctgcac atcgacgacc accacaccgc cgaagacatc ggcatcacac tcggacaagc
     5821 aatccggcag gcactcggcg acaaaaaagg catccgccgt tacggacatt cctacgtccc
     5881 gctcgacgaa gccctcagcc gcgtcgtcat cgacctttcc ggccgccccg gactcgtgta
     5941 caacatcgaa tttacccgcg cactaatcgg acgtttcgat gtcgatttgt ttgaagaatt
     6001 tttccacggc atcgtcaacc acagtatgat gaccctgcac atcgacaacc tcagcggcaa
     6061 aaacgcccac catcaggcgg aaaccgtatt caaagccttc gggcgcgccc tgcgtatggc
     6121 agtcgaacac gacccgcgca tggcaggaca gaccccctcg accaaaggca cgctgaccgc
     6181 ataaaaaacc ataccgtctg aaacacccgc aggcttttca gacggtatcg gaacagataa
     6241 gattacacta cactacaaac agaaaaggag taaacatcat gtccgcaaac gaatacgcac
     6301 aaatcggctg gataggctta gggcaaatgg gtctgcctat ggtaacgcgg ctcttggacg
     6361 gcggcatcga agtcggcgta tacaaccgct ctcccgacaa aactgccccc atctccgcca
     6421 aaggcgcaaa agtttacggc aacaccgccg aactcgtccg cgactatccc gtcattttcc
     6481 tgatggtttc cgactatgcc gccgtgtgcg acatcctgaa cggagtccgc gacggattgg
     6541 ccggcaaaat catcgtcaac atgagcacca tctccccgac cgaaaacctc gccgtcaaag
     6601 cacttgtcga agccgcaggc ggacagtttg ccgaagcacc cgtttccgga tcggtcgggc
     6661 ccgccaccaa cggcacgctg ctgattctgt tcggcggcag cgaagccgtt ttaaacccgc
     6721 tgcaaaaaat attttccctc gtcggcaaaa aaaccttcca tttcggcgat gtcggcaaag
     6781 gttcgggcgc gaaactcgtc ttgaactcgc tcttgggcat tttcggcgaa gcgtacagcg
     6841 aagcgatgct gatggcgcgg cagttcggca tcgataccga caccatcgtc gaagccatcg
     6901 gcggctcggc aatggactcg cccatgttcc aaaccaaaaa atccctgtgg gcaaaccgcg
     6961 aattcccacc cgccttcgcc ctcaaacacg cctccaaaga cctcaacctc gccgtcaaag
     7021 agcttgaaca ggcaggcaac accctgcccg ccgtcgaaac cgttgctgcc agctaccgca
     7081 aagcagtcga agccggctac ggcgaacagg acgtttccgg cgtttacctg aaactggcag
     7141 aacactgatt gccttttcca aacacaatgc cgtctgaaca tatttcagac ggcattttta
     7201 tcaccccacg cttaaaatca gtcccgatta tgactatact ccgcgcaaat ttagtcaata
     7261 tcaagaccaa ttatgaaccg actcgaccaa cttggcaccc gtatcaacct gatttgcaat
     7321 gtcttcgaca aatggattgg gcagcaggat ctgaattaca acctctttgc cgtactttat
     7381 accctggcaa ccgaaggcag ccgcacgcaa aagcatatcg gcgaagagtg gagtctgccc
     7441 aaacagaccg tttcaggcgt atgcaaaacc cttgccggac aagggttgat tgaatggcag
     7501 gaaagcgaac aggaccggcg cgaacggttg ctgtcgctga ccgaaaaagg caaagcccat
     7561 gccgcacctt taacagaaag cgcgcaggaa ttcagcgaca aagagacctt tgcaaaatcc
     7621 cccaaaatcc cctaaattcc caccaagaca tttaggggat ttctcatgag caccttctt
//
LOCUS       DAXHTY010000088         7500 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXHTY010000088 DAXHTY010000000
VERSION     DAXHTY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.088"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            147..1157
                     /gene="dusB"
                     /locus_tag="ACFNMK_001664"
     CDS             147..1157
                     /gene="dusB"
                     /locus_tag="ACFNMK_001664"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC6364889.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCREFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFVDEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVCAAGVPVTLKTRLGWHEDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGDTAGV
                     RIARKHIGWYIGEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAGKTDRWACGYRTN
                     AF"
     gene            1187..1426
                     /locus_tag="ACFNMK_001665"
     CDS             1187..1426
                     /locus_tag="ACFNMK_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC6364890.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            1429..1965
                     /gene="ruvC"
                     /locus_tag="ACFNMK_001666"
     CDS             1429..1965
                     /gene="ruvC"
                     /locus_tag="ACFNMK_001666"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC6364891.1"
                     /translation="MAASVRILGIDPGSRVTGFGVIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVTVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            2016..2903
                     /locus_tag="ACFNMK_001667"
     CDS             2016..2903
                     /locus_tag="ACFNMK_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC6364892.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDAQILKGRNRYDNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPGEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGVQDFYKLHETT"
     gene            3622..4416
                     /locus_tag="ACFNMK_001668"
     CDS             3622..4416
                     /locus_tag="ACFNMK_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC6364893.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYDLPSSLSDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGKLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            4540..7143
                     /gene="pepN"
                     /locus_tag="ACFNMK_001669"
     CDS             4540..7143
                     /gene="pepN"
                     /locus_tag="ACFNMK_001669"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC6364894.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAVADYMLEGETLTIAGVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQTFQFESVTEAVVPSLLRGFSAPVHLNYPYSNDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGVELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLCADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
BASE COUNT         1895 a         1938 c         1982 g         1685 t
ORIGIN      
        1 tgcccgcctg tgagtggcgg ggcaggaaat ctgtgtgcgg attatgccat aaaacgatgc
       61 ccgccccaat cgcgggcgcg tccggagatt ggaaatcctg cttaaaaaat gtacaatgtc
      121 gcactttttt gaaacgcgga cccattatgc acatcggcgg ctattttatc gacaacccca
      181 tcgcacttgc gccgatggcg ggcattaccg acaagccgtt ccgccgcctt tgccgcgagt
      241 ttggcgcggg ttgggcagtg tgcgaaatgc tgaccagcga cccgacgctc agaaatacta
      301 gaaaaacctt gcaccgcagc gattttgtcg atgaaggcgg catcgttgcc gtgcagattg
      361 ccggcagcga tccgcagcag atggcggatg cggcgcgtta caacgtcagc ctcggggcgc
      421 agcttatcga catcaatatg ggctgtcccg ccaaaaaagt gtgcaacgtc caagccggca
      481 gcgcgctgat gcagaacgag ccgctggttg ccgccatttt ggaagccgtc gtctgtgcgg
      541 caggcgtacc cgttaccctc aaaacccgtt tgggctggca cgaagaccat caaaacctgc
      601 ccgtcatcgc caaaatcgcc gaagattgcg gcatcgccgc ccttgccgtc cacggacgca
      661 cgcgcacgca aatgtacaaa ggcgaggcgc gttacgaact catcgccgaa accaaatgcc
      721 gtctgaacat cccggtctgg gtcaacggcg acattacctc gccgcaaaaa gcccaagccg
      781 tcctcaaaca aaccgccgca gacggcatca tgatagggcg cggtgcgcaa ggcaggccgt
      841 ggttcttccg cgatttgaaa cattacgccg aacacggtgt tttaccgcct gccttgagtt
      901 tggcagaatg cagtgccgcc attttgaacc acgtccgcgc catgcacgcg ttttacggcg
      961 acaccgccgg tgtgcgcatc gcacgcaaac acataggttg gtacatcggc gaaatgcccg
     1021 acggcgaaca gacacgtcgt gaaatcaacc gcttggacag tgcggcggcg caatacgaca
     1081 tgcttgcagg ttatcttgaa agacttgccg gaaaaaccga ccgttgggcg tgcggctacc
     1141 gcacaaatgc cttctgaaca cttgattatc ctttgaaagt gcaatcatgc cccataccct
     1201 tcccgatatt tcccaatgca tcagacaaaa tttagaacaa tatttcaaag acctgaacgg
     1261 taccgaacct tgcggcgtgt acgatatggt attgcatcag gtggaaaagc cgctgctggt
     1321 gtgtgtgatg gagcaatgcg gcggtaatca gtccaaagcc tccgtgatgc tggggctgaa
     1381 ccgcaatacc ctgcgtaaga agctgattca acacggtttg ttgtgaatat ggctgcaagc
     1441 gtccgtatct taggcatcga cccgggcagt cgcgtaacgg gtttcggtgt catcgatgtc
     1501 agggggcggg atcattttta cgttgcctca ggctgcatca aaacgcctgc cgatgcgcct
     1561 ctggcagaca ggattgccgt gattgtgcgg cacatcggcg aagtcgttac tgtttacaag
     1621 cctcaacagg cggcggtgga acaggtgttc gtcaacgtta atccggcatc gacgctgatg
     1681 ctcggtcagg ctaggggcgc ggcattggcg gcattggtca gccataagct gcccgtttcg
     1741 gaatacacgg ccttgcaggt caaacaggcg gtagtcggca aaggcaaggc ggcgaaagaa
     1801 caggtgcagc atatggtggt gcagatgctg gggctttcgg gaacgccgca ggcggatgcg
     1861 gcggacggtc ttgccgtcgc gctgacccac gccttacgca accacgggct tgccgccaaa
     1921 ctcaatcctt cggggatgca ggtcaagcgc ggcaggtttc aatagtttca gacggcattt
     1981 gtattttgcc gtctgaaaag aaaatgtgta tcgagatgaa atttatattt tttgtactgt
     2041 atgttttgca gtttctgccg tttgcgctgc tgcacaagat tgccgacctg acgggtttgc
     2101 ttgcctacct tctggtcaaa ccgcgccgcc gtatcggcga aatcaatttg gcgaaatgtt
     2161 ttcccgaatg gagtgaggaa aagcgtaaaa ccgtgttgaa acagcatttc aaacacatgg
     2221 cgaaactgat gctcgaatac ggcttatatt ggtacgcgcc tgccgggcgt ttgaaatcgc
     2281 tggtgcgcta ccgcaataag cattatttgg acgacgcgct ggcggcgggg gaaaaagtca
     2341 tcatcctgta tccgcacttt accgcgttcg agatggcggt gtacgcgctt aatcaggatg
     2401 tcccgctaat tagtatgtat tcccaccaaa aaaacaagat attggacgca cagattttga
     2461 aaggccgcaa ccgctacgac aacgtcttcc tcatcgggcg caccgaaggg ctgcgcgccc
     2521 tcgtcaaaca gttccgcaaa agcagcgcgc cgtttctgta tctgcccgat caggatttcg
     2581 gacgcaacga ttcggttttt gtggattttt tcgggattca gacggcaacg attaccggct
     2641 tgagccgcat tgccgcgctt gcaaatgcca aagtgattcc cgccatcccc gtcagggagg
     2701 cggacaatac ggttacactg catttctacc cggcttggaa atcctttccg ggtgaagacg
     2761 cgcaggccga cgcgcagcgt atgaaccgct ttatcgaaga gcgggtgcgc gaacatcccg
     2821 agcagtattt ttggctgcac aagcgtttta aaacccgtcc ggaaggtgta caggattttt
     2881 acaaattaca cgaaactaca tagtggacta ttaagggaaa attgagtttt gcttaaatgc
     2941 gaaaaattga attatataaa cagaagcatt taataattgc tgttatttta agtgtctaaa
     3001 aaataatatt ttttgatttt aaaaatagat ttcaaagaaa atttggagaa tacatgataa
     3061 atcaatgaat atattctatt gatatggtta atatattaaa attattttcc tttaaaaaca
     3121 taatattata aattattatt caataactgc tattttattc ggtgccagct tgagaaatat
     3181 attgtcaaat atatagacat tataaaatta agtatcaatt taaataaatt tttagaattt
     3241 taatctaaaa aatcaaaaaa ttcactattt tataaaataa acgcttctat attcgttttg
     3301 taaaaaatgc ttggcaagct gtaaaaaatg gcgtacattt cgctacatgg aattacacaa
     3361 caggcaggaa atgccgtctg aaaggatttt ccggtcaatc ttgcgattgg tcggggtttc
     3421 atcggaaacg gtgaaacgaa agtttgccgg cgtagagttg agctacgcgg gtaaagccgc
     3481 aggcgaaagc ctgtattgtt tgtgaagcgt aaatctctga tttgaggtat tggggcaatc
     3541 ctgtgggggg ttgcctcttt ttttatccgc cttttaatga cacaataggc gcaagtctgt
     3601 ttttgaatgc aaggtactgc catgaatacg attttggctt tcgatattga aaccgtaccc
     3661 gatgtgcagg gtatccgtac gctgtatgac ttgccgtctt ccctgtccga cgatgaagtg
     3721 gtgctgtttg cccagcaaaa acgccgtgct cagacaggcg gggattttat gcagcatcat
     3781 cttcatcagg ttgtggcgat ttcgtgctgc atgcgttggg ggcaggataa agtccatgtc
     3841 ggtaccatcg gcgagatgga tgacggcgag gaagtggtca ttgccaagtt tttcgagttg
     3901 attgaaaaac atacgccgca actggtcagt tggaacggcg gcggtttcga tctgccggtt
     3961 ctgcattacc gctccctgat atatggcatc aacgccgcgc gctattggga tatgggcgac
     4021 ggcgatttcg gcgacagccg cgatttcaag tggaacaact acatcagccg ctaccaccaa
     4081 cgtcattgcg atttgatgga tttgctcgcg ctttaccagc cgagggcgaa tgtgccgctg
     4141 gacgatatgg cgaaactgtg cggttttccg ggtaaattgg gtatggacgg cagcaaggtc
     4201 tgggaggcat tccatgccgg taaattgaag gaaatccgca attattgtga aaccgatgcc
     4261 gtgaatacgt atttgatgta tctgcgtttt tgtctggtca gcggcagatt cgacgcggac
     4321 gaatacgaaa tggaaatcaa gcggatcaga aactatcttt ccgcccaaac agaagacaaa
     4381 ccgcattggg cagaatttgt ccaagcgtgg aaatagaaaa actgctgtcc gaaggcggta
     4441 cgcttgtgcc gccgtttcgg tttcagacgg catacgcggg atgcgcccat gccgtctgaa
     4501 gccttttcca gtcaaaccaa acaatattga aagaaaaaaa tgagcaaaac cgtccgttat
     4561 ctgaaagatt accaaacgcc tgcctaccgt attctcgaaa ccgacctgca tttcgatatt
     4621 gccgaaccgc aaaccgtcgt gaaatcccgt ttgacggtcg agccgcagag ggtgggggag
     4681 ccgctggtgt tggacggttc ggcgaaactt ttgtccgtca aaatcaacgg ggcggtggcg
     4741 gattatatgt tggaaggcga gacgctgacg attgcgggcg tgccgtccga acgcttcacc
     4801 gtcgaagtgg aaaccgaaat cctgccggcg gaaaacaaat cgctgatggg gctgtatgct
     4861 tccggaggca atctgtttac ccagtgcgag ccggagggct tccgcaaaat cacgttttac
     4921 atcgaccgtc ctgatgtgat gtccaagttc acaaccacca tcgttgcgga caaaaaacgc
     4981 tatcccgttt tgctctccaa cggcaacaaa atcgacggcg gcgagtattc agacggccgc
     5041 cattgggtga aatgggaaga cccgtttgcc aaaccgagtt atctgtttgc tttggtcgcg
     5101 ggcgatttgg cggtcacgga agactatttc accaccatga gcggcagaaa cgtcaaaatc
     5161 gagttttaca ccaccgaagc ggacaagccc aaggtcggct ttgccgtgga atcgctgaaa
     5221 aacgcaatga agtgggacga aacgcgcttc ggtttggaat acgacttgga tattttcatg
     5281 gtcgtcgccg tgggtgattt caatatgggt gcgatggaaa acaagggttt gaacatcttt
     5341 aacaccaagt tcgtcctcgc cgacagccgt accgccaccg ataccgattt tgaaggcatt
     5401 gaatccgtgg tcggacacga atatttccac aactggacag gcaaccgcgt tacctgccgc
     5461 gattggttcc agctttcgct gaaagaaggg ttgaccgtgt tccgcgacca agaattttcc
     5521 ggcgaccgcg ccagccgcgc cgtgcgccgc atcgagaaca tccgcctgct gcgccagcac
     5581 cagttccccg aagacgcagg cccgaccgcc catccggtgc gccccgccag ctatgaggag
     5641 atgaacaatt tctacaccat gaccgtttat gaaaaaggcg cggaagtggt gcggatgtat
     5701 cacaccttgc tcggcgaaga gggcttccaa aaaggcatga agctctattt ccaacgacac
     5761 gacgggcagg ccgtgacctg cgacgatttc cgcgcggcga tggcggacgc gaacggcatc
     5821 aatctcgacc agttcgcctt gtggtacagc caggcgggta cgcccgtctt ggaagcggaa
     5881 ggtcgtctga aaaacaatag tttcgagtta accatcaaac aaaccgtgcc gcccacgccc
     5941 gatatggcgg acaagcagcc gatgatgatt cccgtcaaag tcgggctgct gaaccgcaac
     6001 ggcgaagcgg tggcattcga ttatcagggc aaacgcgcaa ccgaagccgt gttactgctg
     6061 accgaagccg aacagacctt ccagttcgaa agcgtaaccg aagccgtcgt tccctcgctg
     6121 ctgcgcgggt tcagcgcgcc agtgcatctg aactatccgt acagcaacga cgacctgctg
     6181 ctcctgcttg cccacgacag cgacgccttc acgcgctggg aagccgcgca aacgctctac
     6241 cgccgcgccg tcgccgccaa ccttgccgcg ctttcagacg gcgtcgagtt gccgaaacat
     6301 gaaaaactgc ttgccgccgt cgaaaaagtc atttcagacg acctcttgga caacgccttc
     6361 aaagccctgc ttttgggcgt tccgtctgaa gccgaactgt gggacggcgc agaaaacatc
     6421 gacccgctgc gctaccatca ggcgcgcgaa gccttgttgg atacgcttgc cgtccgcttc
     6481 ctgccgaaat ggcacgaatt gaaccgtcag gcggcgaagc aggaaaacca aagctacgag
     6541 tacagccccg aagctgccgg ctggcgcacg ctgcgcaacg tctgccgcgc cttcgtcctg
     6601 tgcgccgacc ccgcgcacat cgaaaccgtt gccgaaaaat acggcgaaat ggcgcaaaac
     6661 atgacccacg aatggggcat cctgtccgcc gttaacggca acgaaagcga tacgcgcaac
     6721 cgcctgctgg ctcagtttgc cgacaagttt tcagacgacg cgctggtgat ggacaaatat
     6781 ttcgccctcg tcggctcaag ccgccgcagc gacaccctgc aacaggttca aaccgccttg
     6841 cagcatccga agttcagcct cgaaaatccc aacaaagccc gctcgctcat cggcagcttc
     6901 agccgcaacg tcccgcattt ccacgcagaa gacggcagcg gctaccgctt catcgccgac
     6961 aaagtcatcg aaatcgaccg ctttaacccg caggtcgccg cccgcctggt gcaggcgttc
     7021 aacctctgca acaagctcga gccgcaccgc aaaaacttgg tgaaacaagc attgcagcgc
     7081 attcgggcgc aggaaggatt gtcgaaagac gtgggcgaaa tcgtcggcaa aattttggat
     7141 tgaggccgtc aaacagaaaa acaatgccgt ctgatatagt tacacacacg ttttatcatc
     7201 acttccccat cgttttatta cgcaatggca aaacggccgc aaagtcatat taattatagt
     7261 ggattaacaa aaatcaggac aaggcgacaa agccgcagac agtacagata gtacggtaag
     7321 gcgaggcaac gctgtactgg tttttgtgaa tccactatat tgctagaagt gcaccgatta
     7381 aggttaatta aaatacgacc cttatcccaa tattaagaaa aacagcaatc aaaataaact
     7441 tgattgctgt ttttcagttt aagccatgaa tgtaattagg ctccttatgc agatggcgtt
//
LOCUS       DAXHTY010000089         7472 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXHTY010000089 DAXHTY010000000
VERSION     DAXHTY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.089"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            140..721
                     /locus_tag="ACFNMK_001670"
     CDS             140..721
                     /locus_tag="ACFNMK_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6364895.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(783..1637)
                     /gene="folD"
                     /locus_tag="ACFNMK_001671"
     CDS             complement(783..1637)
                     /gene="folD"
                     /locus_tag="ACFNMK_001671"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC6364896.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQNNLHTPCLAVVLVGGD
                     PASAVYVRNKKTSCQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDSSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1860..1937
                     /locus_tag="ACFNMK_001672"
     tRNA            1860..1937
                     /locus_tag="ACFNMK_001672"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1895..1897,aa:Pro,seq:tgg)
     gene            1962..2038
                     /locus_tag="ACFNMK_001673"
     tRNA            1962..2038
                     /locus_tag="ACFNMK_001673"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1996..1998,aa:Arg,seq:tct)
     gene            2066..2141
                     /locus_tag="ACFNMK_001674"
     tRNA            2066..2141
                     /locus_tag="ACFNMK_001674"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2099..2101,aa:His,seq:gtg)
     gene            2277..2783
                     /locus_tag="ACFNMK_001675"
     CDS             2277..2783
                     /locus_tag="ACFNMK_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFC6364897.1"
                     /translation="MVDAWSVPDFESKICPPEALSERLARLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKDGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2780..4558
                     /locus_tag="ACFNMK_001676"
     CDS             2780..4558
                     /locus_tag="ACFNMK_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC6364898.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAVSVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRPDNRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4671..5495
                     /locus_tag="ACFNMK_001677"
     CDS             4671..5495
                     /locus_tag="ACFNMK_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC6364899.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(6258..7046)
                     /locus_tag="ACFNMK_001678"
     CDS             complement(6258..7046)
                     /locus_tag="ACFNMK_001678"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC6364900.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAEPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(7260..7454)
                     /gene="thiS"
                     /locus_tag="ACFNMK_001679"
     CDS             complement(7260..7454)
                     /gene="thiS"
                     /locus_tag="ACFNMK_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC6364901.1"
                     /translation="MNIILNGEPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         1594 a         1924 c         2236 g         1718 t
ORIGIN      
        1 atgtatggcg taaaggaagc gttttaacgc cgtattggga aaatgtaaag ttctttatcg
       61 ggatttcttt aagtattggc gttttcggtg tcgtggcggt agaatcgggg tcagttaaga
      121 acaattatca ggaacggata tggaagaaaa aaacgactac accgacgcgg catccgacaa
      181 ccgcaacggg caggaaattg aagtcggaat tgccggcgca ggcgaccgga tacttgccgc
      241 gctgctgaac caattgttta cctttttggt tttgttggtg ccgtttgccg gactgattgc
      301 tttcgccatc aagaatgaag gcaggattgg cggcagtgag gaaatattcg ggctgctttt
      361 gggcatgact tccttttggg taggactggc gggcattttg gtttataccg tcgttcaaat
      421 ctactatatg agtcgggacg ggcagtcatt gggtaagaag atcatgagaa tcagggtgtt
      481 gaaaaccgac ggccgcaatc cgggttttgt cggcacggtt ttggtacgcg aaatcgcatg
      541 gtcggttttg gttgccatta ttgccgccgt tatcggtctt gcagtaggtg acaacggaga
      601 aaacgccatc aacctgctgg cattccttgc caactttgtc ctgctcttta tggtcaaacg
      661 cgaccgccgc acgctttacg acatactggc ggatacggtt gtcgtcaagc tgcccaaata
      721 aacggacacg gcaaaatgcc gtctgaaagc ctttcagacg gcatttttat cttcagaacc
      781 gctcaagcat cgtgcagtga agccgcgtgc agggtgtttt ccatcaatgt ggcaatcgtc
      841 atcggaccca cgccgccggg aacgggcgta atcatcgccg cccgttcttt tgccgtttca
      901 aattccacgt cgccgcacag gctgctatcg tccaaacggt tgatgcccac atcaataacg
      961 accgcgccag gtttgatcca ttcgcctttg acaaagttcg gaatgcctac gccgaccacc
     1021 aaaatatcgg ctccggcaac ctcgtctgtc agattttcgg ttgcgctgtg gcagaccgtt
     1081 accgttgcgc gcgccagcag cagttccaaa gcctgcgggc ggccgacgat attcgacgcg
     1141 ccgaccacga ccgctttttt ccccttcgga tcaatgccgt aagcttccaa aagcgtcatc
     1201 acgcccttgg gcgtacacgg gcgcatcagc ggcattttga ccgccagcct gccgacattg
     1261 taaggatgga agccgtccac gtccttatcc ggcgaaatac gttccaaaac cgcctggctg
     1321 tcgaggtgct tcggcagcgg tagctgaacc agaataccgt ccacttcgga atcggcattc
     1381 aggcggtcga ccagtgccag cagttcttcc tgcgatgttg attcgggcag ctcgtaagac
     1441 agtgatttga tgccgcattt ttggcaggag gttttcttgt tgcggacata aaccgcgctg
     1501 gcagggtcgc ctccgaccaa aaccacggcc aggcaaggcg tgtgcagatt gttctgttgg
     1561 cgttgcgcca ccgcttcggc aaccgcctgc aggcgttttt gcgaaacttc tttaccattg
     1621 atcagttgtg ccgacatatc cgtccttttg ttaaacaggt agaaaatgct gaaaattatc
     1681 gcattttccg tttgttttta cagcaggatg ccgaaaataa atttgccctg aaataaaata
     1741 cggctgtttg gacggattaa atattttttc aaatcaaaaa actatatttt tcatcttggt
     1801 gtcttgaaaa taaatgttga cgcatctttc cctgagttgt atagttcgct tcttcaagtc
     1861 ggggcgtagc gcagcccggt tagcgcatct gctttgggag cagagggtcg tgagttcgaa
     1921 tcccaccgcc ccgaccaatc aatcagcacg aatcggcatg agcgcccgta gctcaaccgg
     1981 atagagcacc gaccttctaa gtcgggggtt acaggttcga ttcctgtcgg gcgtgccaag
     2041 aatttgattg aaaaccgttt ttatggtggc tgtagctcag ttggtagagc cccggattgt
     2101 gattccggtt gtcgtgggtt cgagccccat cagccacccc agataaaggc ccgtactttt
     2161 cggtacgggt cttttcccgt ttgcgcgtac tgtccgcgcc gccggctttg cctttcgcat
     2221 atgcttctgt atcatgtggg tttttctgtc ttttatggtt tttgtcggag gctttgatgg
     2281 ttgacgcttg gtctgtccct gattttgaat cgaaaatctg tccgcccgag gcattgtccg
     2341 aacggttggc tcggttgccg cgcccgctgg tgtttaccaa cggctgtttc gacatcctcc
     2401 acagggggca cgttacttat ctggcgcagg cgcgttcggc aggggcggcg ttggtgctgg
     2461 cgttgaatac cgatgcttcg gtgcggcgtt tgggcaagga cggcgaccgc ccgattaatc
     2521 ctttggagaa ccgtgccgcc gttgccgccg ctttggaaag tgtggatttg gtaacgtggt
     2581 ttgacgggga tacgccggcg gcgttgattg aggcggtcaa acctgagatt ttggtcaagg
     2641 gcggcgattg ggctgcggat aagattgtcg gtgcggcaga aacgttggcg cgcggcggtc
     2701 aggtgttttc aattccgttt ctgcaccaga cttcgacaac gaagactttg gcaaaaatcc
     2761 gtgcggcaga gggcggaaaa tgacggtttt gaagctttcg cactggcggg tgttggcgga
     2821 gcttgccgac ggtttgccgc aacacgtctc gcaactggcg cgtatggcgg atatgaagcc
     2881 gcagcagctc aacggttttt ggcagcagat gccggcgcac atacgcgggc tgttgcgcca
     2941 acacgacggc tattggcggc tggtgcgccc attggcggtt ttcgatgccg aaggtttgcg
     3001 cgagctgggg gaaaggtcgg gttttcagac ggcattgaag cacgagtgcg cgtccagcaa
     3061 cgacgagata ctggaattgg cgcggattgc gccggacaag gcgcacaaaa ccatatgtgt
     3121 gacccacctg caaagtaagg gcagggggcg gcaggggcgg aagtggtcgc accgtttggg
     3181 cgagtgtctg atgttcagtt ttggctgggt gtttgaccgg ccgcagtatg agttgggttc
     3241 gctgtcgcct gttgcggcag tggcgtgtcg gcgcgccttg tcgcgtttag gtttggatgt
     3301 gcagattaag tggcccaatg atttggttgt cggacgcgac aaattgggcg gcattctgat
     3361 tgaaacggtc aggacgggcg gcaaaacggt tgccgtggtc ggtatcggca tcaattttgt
     3421 cctgcccaag gaagtagaaa atgccgtttc cgtgcaatcg ctgtttcaga cggcatcgcg
     3481 gcggggcaat gccgatgccg ccgtgctgct ggaaacgctg ttggtggaac tggacgcggt
     3541 gttgttgcaa tatgcgcggg acggatttgc gccttttgtg gcggaatatc aggctgccaa
     3601 ccgcgaccac ggcaaggcgg tattgctgtt gcgcgacggc gaaaccgtgt tcgaaggcac
     3661 ggttaaaggc gtggacggac aaggcgtttt gcacttggaa acggcagagg gcaaacagac
     3721 ggtcgtcagc ggcgaaatca gcctgcggcc cgacaacagg ccggtttccg tgccgaagcg
     3781 gcgggattcg gaacgttttc tgctgttgga cggcggcaac agccggctca agtgggcgtg
     3841 ggtggaaaac ggcacgttcg caaccgtcgg tagcgcgccg taccgcgatt tgtcgccttt
     3901 gggcgcggag tgggcggaaa aggcggatgg aaatgtccgc atcgtcggtt gcgccgtgtg
     3961 cggagaattc aaaaaggcac aagtgcagga acagctcgcc cgaaaaatcg agtggctgcc
     4021 gtcttccgca caggctttgg gcatacgcaa ccactaccgc caccccgaag aacacggttc
     4081 cgaccgctgg ttcaacgcct tgggcagccg ccgcttcagc cgcaacgcct gcgtcgtcgt
     4141 cagttgcggc acggcggtaa cggttgacgc gctcaccgat gacggacatt atctcggggg
     4201 aaccatcatg cccggtttcc acctgatgaa agaatcgctc gccgtccgaa ccgccaacct
     4261 caaccggcac gccggtaagc gttatccttt cccgaccaca acgggcaatg ccgtcgccag
     4321 cggcatgatg gatgcggttt gcggctcggt tatgatgatg cacgggcgtt tgaaagaaaa
     4381 aaccggggcg ggcaagcctg tcgatgtcat cattaccggc ggcggcgcgg caaaagttgc
     4441 cgaagccctg ccgcctgcat ttttggcgga aaataccgtg cgcgtggcgg acaacctcgt
     4501 cattcacggg ctgctgaacc tgattgccgc cgaaggcggg gaatcggaac atacttaaaa
     4561 aatacaccct ttttcaacac ggggcggcat ccgtccgaat ccgtccggat tctgccgccc
     4621 gacagactgc cgcccaggcg ggcggggttt caaacattga ggaaattcta atgaaatggc
     4681 tcttcaccct tttggcggta ctcaatattg ccgtattcgg cggcacggtg ggctataaat
     4741 tagccgttaa agcgacgggc ggcgtgccgg aaaaccgggc cgtcgaaaac acgccccccg
     4801 caacgcccgc cgccgacaat gcggcggcat ccgtggagga tacggcggca ctgctcaaac
     4861 cgggcgacat cctgaccgaa gagcaggccg agcagttgcg cttgaaaaag gaagcggaac
     4921 agaaaaaact gagggagaaa aaacagcgtg aagaaaaagc ccgccgcgaa aaactcgccg
     4981 ccgaaaaggc gcaggcggaa cgcgaaaacg gcgcggcgga tgccttatgc gccgcgcagg
     5041 caagcctcac gatggacgaa gatgactacc accgcatcaa aggacttttg ggcaaatggt
     5101 cgcacgttgc cagcaggagc gtcgaaaaac gcaccgccaa agccaaacct gccgacaaaa
     5161 cctaccgcgt cgtcctgccc gtttccgccg atgccgaaaa tcaggcggcg gagctgtctg
     5221 ccaaaggttt caaccccata ccgtttgacg gcgcattgag tttgggtgtc ggcaacagcc
     5281 gggaaaacgc ccaagccctg caaaaccggc ttgccggcgc cggattcggc ggggcgcata
     5341 ttgtcgaaca ctttgccgaa gccgacaggc aggacgattc tttgagcgtg tcgcgtatga
     5401 cggttttgtt taccggcgtg aatgccgccg atgcggacga aattcgtaaa atcacgtccc
     5461 tatacggcaa actgaacctc aagtcttgca aataggcgcg aaagccggac gataaacgcc
     5521 aaacccaccg cgaaggtggg tttggcgttt cggttttgag tatctgatat ataaaaaggg
     5581 aatgtatgtc tcgtatatgc cgactccaag tgtgaaagtg atgatgggga aatatggtgt
     5641 ttaatggttc ggaacggacg gcttccgagt ggttttgatt atacgctaat aataataatt
     5701 atcaataaaa atttaaaaat aaatgcagat tttttgtaat acgatgaaaa ataaacaaat
     5761 taacctgtcc gccgatgttt gtgattcggg tcggcaaact ttcattgtgt cggacaaatc
     5821 ggggtcggca gtcatagctg agggcttcgt gcctgacgga aataaggttg cccgtgtttg
     5881 aacatccgtc cgattgggcg cggacagggt tgtcattcgg gggtgtttga gcgtggattc
     5941 ggggatgcga aagcggtaaa accgttgcgg atgaaggctt acggacggct ttaggtattt
     6001 ttaagtgtat tcaaacaaca acaaaacgct tcttctattg ccgtatttag ggcttgcgcg
     6061 gcagcctgca caccgtggag gccgagtgtt tcgttggttg cggcatcggg acgggcggca
     6121 aggttttccg gccggttttt ccattttttc cgacaaagcc gtgatgccgg ggaatggcgg
     6181 tttgtccgtt tcggaccgcc gccgttttga gccgcgatgc cgtctgaacc gttcagacgg
     6241 catttttgcc gcctttttca atattccgcc gaatgccaaa acggctgtcc gactgtcggc
     6301 gtgctggctt gcgctttgtc gcgtgcttcg accggttcgg cttcaaatgc cagccgtccg
     6361 gattcgacgg cgagtgcgaa ggcgcgtgcc atattgaccg gatcgccgct gcgggaaacg
     6421 gcagtattca aaagcacgcc gtcaaaaccc cattccatca cttgtgccgc ctgtgagggc
     6481 aaacccaagc ccgcgtcgat aatcagcggc gtgtcgggca ggcgttcgcg caggacgttc
     6541 aacgcgtagg cgtgaaccgc gcccaaaccc gtgccgatcg gagccgccca cggcattaac
     6601 gcctgacagc ccgcgtcgag caggcggcgg caggcaatca ggtcttcggt gcaataaggc
     6661 agcactttga agccgtcttt aatcaggatt tccgccgctt cgacaagttg gaacacatcc
     6721 ggctgcaagg tgtcgtcgtc gccgatgagt tcgagtttaa tccaatcggt ttcaaacact
     6781 tcgcgcgcca tttgcgccgt cgttaccgct tcctgcacgc tttggcagcc tgccgtgttc
     6841 ggcaggacgg gaacgccggt ttcttgaagc agcgaccaaa acccctgacc gtgcgcctcg
     6901 ccgccgcttc ccgtgcggcg cagcgagacg gtaatcatcg cgggttgggc gatacggacg
     6961 gattgtttga ggatttcagg ggtcgggtag gcagccgtgc cgagcagcag ccgcgagggg
     7021 aaagtttctc cgtatagggt gagcataatg ggttcctttg tatagtggat taacaaaaac
     7081 cagtacggcg ttgcctcgcc ttagctcaaa gaaaacgatt ctctaaggtg ctgaagcact
     7141 aagtgaatcg gttccgtact atccgtactg tctgcggctt cgtcgccttg tcctgattta
     7201 aagttaatcc accataacgt tgttttgggg acaggggtcg tctgaaaagg caaaaccgcc
     7261 tagccgccga ccaccggccg cacgatatcg attttgtcgt tttcgtttaa aaccgtttcc
     7321 gcatacgcgc ctttggggac gaaaacggtg ttgaccgcca cggcaaaggg cttttgcggc
     7381 gcggtttggg cgatgaggtc ggcaacgctt gtgccgtgaa gttcggcggg ttcgccgttt
     7441 aagatgatgt tcatggtttc tcgatgtttc tg
//
LOCUS       DAXHTY010000090         7324 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXHTY010000090 DAXHTY010000000
VERSION     DAXHTY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.090"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(99..1016)
                     /gene="thrB"
                     /locus_tag="ACFNMK_001680"
     CDS             complement(99..1016)
                     /gene="thrB"
                     /locus_tag="ACFNMK_001680"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC6364902.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLPR
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWVRTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQEGEMTFIKDPNAFRNLLLSLG"
     gene            complement(1053..1781)
                     /gene="ubiG"
                     /locus_tag="ACFNMK_001681"
     CDS             complement(1053..1781)
                     /gene="ubiG"
                     /locus_tag="ACFNMK_001681"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC6364903.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1933..3174
                     /locus_tag="ACFNMK_001682"
     CDS             1933..3174
                     /locus_tag="ACFNMK_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC6364904.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRQKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3326..4024
                     /locus_tag="ACFNMK_001683"
     CDS             3326..4024
                     /locus_tag="ACFNMK_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6364905.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVNGLNRQNITPAVYATKFD
                     HSIPEYALIEPHLVDQRRTLKKLRSFLFSSRLAQTRKNSAAKLVACHHADYADLFICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMRRELVGLYGVPPEKIQ
                     VAPPPQIRNASFHNPEKLPTCAPNTALPTMKPFSCSHRPATRAKVWNCLPTFSNIPAC
                     PSSSPLPAPRSPAL"
     gene            3907..4380
                     /locus_tag="ACFNMK_001684"
     CDS             3907..4380
                     /locus_tag="ACFNMK_001684"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6364906.1"
                     /translation="MFPSTGHTRKGLELLADFFEHTGLPVKLAVAGSPLPRPMKNVVG
                     LGFCTDMPELYRAADFTIMASLYEPFGLVGVESVLCGTRVVLSENMACTEVMNEEAGF
                     FFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYNPSLSHHIDRLTDMLASV"
     gene            4656..5219
                     /gene="gmhB"
                     /locus_tag="ACFNMK_001685"
     CDS             4656..5219
                     /gene="gmhB"
                     /locus_tag="ACFNMK_001685"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC6364907.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KLGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            5248..5991
                     /locus_tag="ACFNMK_001686"
     CDS             5248..5991
                     /locus_tag="ACFNMK_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6364908.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGTENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            5988..6675
                     /locus_tag="ACFNMK_001687"
                     /pseudo
     CDS             5988..6675
                     /locus_tag="ACFNMK_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
BASE COUNT         1742 a         2375 c         1725 g         1482 t
ORIGIN      
        1 gaaaataccc ggctatcgtc tcattttcgt tttaatcagc cataaaaatg ccgtctgaaa
       61 ccctttcaga cggcatttct gtcaaacgcc ggacacactc aacccaaact caatagcagg
      121 ttgcggaacg cgttcgggtc tttgataaac gtcatctcgc cctcctgcgg aaaatgaaaa
      181 tccaacaggc gcgacaccca aaaacggatg cagccggcgc gttgggcggt cgggaaatac
      241 gccttttctt cggcactcaa ggggcgcacg ccctcataac cgccgataaa cgcctttttc
      301 aacgcctcat ccaacttatt gtccgccgtc cttacccaat cgttgaccgc aatcgccaag
      361 tcatacataa aattgccccg gcaggcataa tagaaatcga tgaaacccga tacctgaccg
      421 ccgtcaagca acacattatc cttgaacaaa tcggcgtgga tgatgcccga aggcagatgg
      481 ttgccgagat tgtccttcag cgcatcgatt tcggaacaca gcagcgcggc atcgtctcgc
      541 ggcaggacgg gcagcagtcg ggcgcacgcc tctgtccacc acgcatcata acgcgggttt
      601 tccatttcca aagggaaatc ggcggcggca aggtgcattt tcgccaacat cgcgccggta
      661 tggaaacact gttccgccgt cggcagcgcg gtgtccgaac ctttcaggca ggcaaccagg
      721 catgcgggct tgcccgccaa aacggaatca agccggccgt ctttgcgcgc aaccggcgcg
      781 gcaaccgcca cgcccttcat actcaaatgc cggttaagct ccagaaaaaa cggcagctct
      841 tcctgtttca acacttcaaa cacggtcagc acataacgtc ccgaagtcgt cgtcagaaaa
      901 taattgctgt tggtaatccc ctgcgcgatg ccctgcaggg aaacaaattc ccccaaatcg
      961 taaccgctca ggaagccgcg catttcatca tcggaaacac tggtatagac agacatatca
     1021 actcacttgc tcaaaaacgg gcaaacccgc cgtcagaacg ccggacggca ggcaaacata
     1081 taattcacat cggtcgaatc gcacagggca taagtttgcg acaacacatg gtaagtcata
     1141 cccttcgtat ccgccacatc caagcctgcc tgacggcaca tccgcgccag ctcggcaggc
     1201 gcgatgaatt ttttccagtc gtgcgtgcct ttggggacaa acttcaacag atattccgcc
     1261 gccacaatca gatgcaggta cgatttcggg tttttattga tggtggaaaa aaacaccatg
     1321 ccgtccggtt tgaccagctt ggcacaagca cgcacgatgg cggcgggatc ggggacgtgt
     1381 tccatcattt ccatgcacgt taccacatcg aacgagtgcg gttccgcctc ggcaaggtct
     1441 tccacgcgga tacattcgta ttcgatatcg gcgacattgt tcaaagccgc gtgcaggcgg
     1501 gcggtttcca gcgactgctc cgccatgtcg atacccttga caaacgccgc gccgcgccgc
     1561 gccatgcttt ccgcaaggat gccgccgccg cagcccacgt ccaaaacccg tttgccgcac
     1621 aaatccgcgt gtccgtcgat ataatcgagc cggagcggat tgatgtcgtg caaggttttg
     1681 aactcgcccg acttgtccca ccatttgtcg gcaatccggc tgaatttggc gatttccccc
     1741 tcatcgacat tatatttttt gtcggacatt ttccctccca tctgacgaac cgcccactcc
     1801 aaaacccaag attatactat ggaacacaat gccgtctgaa agccttttcg ggcggcgcgg
     1861 cgcaaatgcc ttacaaatcc ttacacttta cggcataatg gcggctcgct ttttctggca
     1921 gaaagacaaa atatgcccac caaaacccct tcactgttcg gcggcgcgat gattatcgcc
     1981 ggcacggtca tcggcgcagg tatgctcgcc aacccgaccg ccacatccgg cgtatggttt
     2041 accggctcgc tggccgtgtt gctgtacacc tggttttcta tgctttccag cggcctgatg
     2101 attttggaag tcaacaccca ttatccgcac ggcgcaagtt tcgacacgat ggtcaaagac
     2161 ctgctcggac gcggctggaa catcatcaac ggcatcgccg tcgccttcgt tttatacctg
     2221 cttacttacg cttatatctt cgtcggcggc gacctgaccg ccaaaggctt aggcagcgcg
     2281 gcaggcggca atgtttcact caccgtcgga caactcgtct tcttcggcat tctcgccttt
     2341 tgcgtatggg catccgcacg cttggtcgac cgattcacca gcgtcctcat cggcggcatg
     2401 gtattaacct ttatttgggc aaccggcggc ctgattgccg atgccaaaat gcccgtcctg
     2461 ttcgacaccc aagcccccgt cggcaccaac tactggattt acgccgccac cgccctgccc
     2521 gtctgccttg cgtcattcgg tttccacggc aacgtctcca gcctgctcaa atactttaaa
     2581 ggcgacgcgc ccaaagtggc taaatccatc tggacgggca cactgattgc gctggtaatt
     2641 tacgtcctct ggcaaaccgc catccaaggc aacctgccgc gcaacgagtt cgcccccgtg
     2701 attgccgccg aagggcaagt ctccgtcctg attgaaaccc tgtccaaatt cgcccaaacc
     2761 ggcaatatgg acaaaatatt gtccctgttt tcctatatgg cgatcgccac ctcgttttta
     2821 ggcgtaacgc tcggactctt cgactacatc gccgacatct tcaaatggaa cgacagcgtg
     2881 tccggccgca ccaaaaccgc cgcgctgacc ttcctgccgc ctctaatttc ctgcctgctc
     2941 ttccccaccg gctttgttac cgccatcggc tacgtcggcc tggcggcaac cgtctggaca
     3001 ggcatcatcc ccgccatgct gctctaccgt tcgcgccaaa aattcggcgc aggcaaaacc
     3061 tataaagttt acggcggctt gtggctgatg gtttgggtct tccttttcgg catcgtcaac
     3121 atcgccgcac aggtattgag ccaaatggaa ctcgtccccg tatttaaagg ataaaggcaa
     3181 aatgccgtct gaagcccgcc ggcagcttca gacggcattg ccgcaacaaa cggcaaccgt
     3241 gttccggcac acagcgcatt accctacccc cttacgcaca aatcccgccc cgacagtcgc
     3301 gggacgcaac cataaaggaa caatgatgaa gctcaaaata gacattgcaa ccaacaactt
     3361 caaacacggc ggcggcacgg aacgctacac attggatttg gtaaacggtc tgaacaggca
     3421 aaacatcaca ccggccgttt atgcgacgaa atttgatcac agcattcccg aatacgccct
     3481 aatcgaaccc catcttgtcg atcaacgccg gacgctgaaa aaactgcgct cgttcctctt
     3541 ttcaagccgg ctcgctcaaa ccagaaaaaa cagtgccgcc aaactggttg cctgccacca
     3601 cgccgattac gccgacctct tcatctgcgg cggcacacac ttgggctacc tgcaccatat
     3661 ggcgcaaaaa ccgaacctgc tcgaccgcct cgccatacgc cgcaaccgca gcaactacgc
     3721 caccgccaaa ctgattatgg cgcattccca tatgatgcgg cgcgaactgg tcggactgta
     3781 cggcgttccc cccgaaaaaa tccaagtcgc cccccccccg cagatacgga acgcttcttt
     3841 ccacaacccg gagaaactgc cgacctgcgc gccaaatacg gctttgccga ccatgaaacc
     3901 gttttcctgt tcccatcgac cggccacacg cgcaaaggtc tggaactgct tgccgacttt
     3961 ttcgaacata ccggcctgcc cgtcaagctc gccgttgccg gctccccgct cccccgccct
     4021 atgaaaaacg tcgtcggact gggcttctgc accgatatgc ccgaactcta ccgcgccgcc
     4081 gactttacca ttatggcctc cctgtatgaa cccttcgggc tggtcggtgt cgaatccgtc
     4141 ctatgcggca cacgcgtcgt cctctccgaa aacatggcat gtacagaggt catgaacgaa
     4201 gaagccggat tctttttctc acgccaaaac ccggaaaccc tggcgcaagc cgttgcccaa
     4261 gccgtcagcc ttaaaaaaca gggcggacac cgcctgtccg acccgatgcg tgcactgaac
     4321 tacaacccgt ccctgtcgca ccacatcgac cgactgaccg acatgctggc atccgtataa
     4381 caaaatgccg tctgaacgag ttttcagacg gcattgctac gttatagtgg attaacaaaa
     4441 atcaggacaa ggcaacgaag ccgcagacag tacaaatagt acggaaccga ttcactcggt
     4501 gcttgagcac cttagagaat cgttctcttt gagctaaggt gaggtaacgc cgtactggtt
     4561 tttgttaatc cactatatca cgcggcacag gcgcatcact gccgatattt cagtataatg
     4621 ccacccccga cctgccccaa tccaaaggaa acgcgatgaa actcatcatt ctcgaccgcg
     4681 acggcgtcat caatcaggac cgcgacgact tcgtcaaatc cgttgacgag tggatacctg
     4741 tcgaaggcag catggatgcg gtggcatttc tgacgcaggc aggctacacc gtcgccgttg
     4801 ccaccaacca atccggcatc gggcgcaaat attttaccgt tcaaaacctt accgaaatgc
     4861 acgccaaaat gcaccgcctc gtccgtcagg caggcggcga aatcaacggc atctggttct
     4921 gcccgcacac cgatgccgac aactgcaact gccgcaagcc caaactgggt atgattgaag
     4981 acatcatcgg acgcttcaac gcccaagcct cggaaacctg gctggtcggc gacagcctgc
     5041 gcgatttgca ggcaatcgat gccgtcggcg gcaaacccgc gctggttctg accggaaaag
     5101 gcaaaaaaac gctctcccaa cacggacacg aattgcccga acacacacag gttttcgata
     5161 ccctgctcga tttctcacaa tacatcatgc aggaaaacgc cgcaccgcaa gccgactgaa
     5221 cataccgcat tccgacaagg caaaaccatg ctcatcatcc gcaacctgat ttactggctg
     5281 atactctgtt ccaccctgat tttcctcttt ccctttatgc tgctcgcctc gcctttccga
     5341 gacggggcgc acaagatggc gcgggtctgg gtcaaaatcc tcaacctctc gctcaaacac
     5401 atcgtcgggc tcaaataccg catcatcggc acggaaaaca tccccgaccg ccccgccgtc
     5461 atctgcgcca aacaccaaag cggctgggaa acgctcgccc ttcaggacat ttttccgccg
     5521 caggtttacg ttgccaaacg cgagttgttc aaaatcccct ttttcggctg gggcttgaaa
     5581 ctggtcaaaa ccataggcat agaccgcaac aaccgccgcg aagccaacga gcagctcata
     5641 aaacaggggt tggcgcgcaa aaacgaaggc tattggatta ccattttccc cgaaggcacg
     5701 cgccttgcgc ccggaaagcg cggcaaatac aaactcggcg gcgcgcgcat ggcgaaaatg
     5761 tttgagatgg acatcgtccc cgtcgccctc aacagcggcg aattttggcc gaaaaactcc
     5821 tttctgaaat atccggggga aatcaccgtc gtcatctgtc cgaccatccc gcacgcaagc
     5881 ggcagcgaag ccgaattgat ggaaaaatgc gaacatctca tcgaaacgca gcagccgctc
     5941 atttccggcg caggtccgtt tgccgccaaa atgccgtctg aaaccgcatg accgcctttg
     6001 tccacaccct ttcagacggc atggaactga ccatagaaat caaacaccgt gccaagaaaa
     6061 acctgattat ccgccctatc ggcacacata ccgtcagcat cagcgtccca ccgcacctgt
     6121 ccgccgccgc cctcaaccgc tggctgcgcg acaacgaagc cgtcctgcgg cgaacactgg
     6181 caaaaacacc gccgcacaat actgcaaacc ggctgcccga acatatctag ttccacggca
     6241 gacaacttgc cctcaccacc catcaagaca cgcaaatcct actgatgccg tctgaaatcc
     6301 gcgttcccga agacacacac gacaaacagg cggcattgtt ggcaaccttt ctgaaacggc
     6361 aggcgcacag ttacctgatt ccccgcctcg aacgccacgc ccgcaccaca caactgttcc
     6421 ccgcctcctc ctcgctgacc tctcaaaacc ttctggggcg tgtgccgcaa aaccacaggc
     6481 atacgcttca actggcggct ggtcggcgca ccggaatacg ttgccgacta tgtctgcata
     6541 cacgaactct gccacctcgc ccatccagac cacagccccg ccttttggga actgacccgc
     6601 cgcttcgccc cctacacgcc cgaagcgaaa cagtggctca aaatccacgg cagggaactt
     6661 ttcgccttgg gctgacgcgg tcccgggccg gcccgccgct ttcagacggc atccgtgccg
     6721 gaacaggcac gcgcccgccc gattcaaacc gcgatgacgc tttgccgccg gttcggggca
     6781 ggatggcggc acacgcgccg tctgccgcgt ttcatttcac accgctcttc cgaaacccgc
     6841 ccggtccgac gtgcggtatg aaacgcttaa gctggcgcga agtcttttac tgatttgccc
     6901 gcgaaaatgc cgtctgaaag gttttcggac ggcatttttt tgcgtttccc aggatggcgg
     6961 cggattcgta aaaggcggtc agggtggatt gtaggatggg ttgagacctg ccgaatccgc
     7021 cgcatctgcc aaatctaccg ccgtcattcc tacgaaagtg ggaatctaga acgcggggtt
     7081 aagaaaacct gcaccgcgtc attcccacga acctacatcc cgtcattccc acgaaagtgg
     7141 gaatccagga cgaaaaatct ccagaaaccg ttttacccga taagtttccg caccgacaga
     7201 cctagattcc cgcctgcgcg ggaatgacgg gatttgaggt ttctgttcgc gtcattccca
     7261 cgaacctgca tcccgtcatt cccacgaaag tgggaatcta gttttgtcgg tgcggaaact
     7321 tatc
//
LOCUS       DAXHTY010000091         7201 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXHTY010000091 DAXHTY010000000
VERSION     DAXHTY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.091"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            325..1770
                     /gene="zwf"
                     /locus_tag="ACFNMK_001688"
     CDS             325..1770
                     /gene="zwf"
                     /locus_tag="ACFNMK_001688"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC6364909.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAARGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            2050..2745
                     /gene="pgl"
                     /locus_tag="ACFNMK_001689"
     CDS             2050..2745
                     /gene="pgl"
                     /locus_tag="ACFNMK_001689"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC6364910.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            2726..3712
                     /locus_tag="ACFNMK_001690"
     CDS             2726..3712
                     /locus_tag="ACFNMK_001690"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC6364911.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            3762..4610
                     /gene="hexR"
                     /locus_tag="ACFNMK_001691"
     CDS             3762..4610
                     /gene="hexR"
                     /locus_tag="ACFNMK_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC6364912.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            4731..6377
                     /gene="pgi"
                     /locus_tag="ACFNMK_001692"
     CDS             4731..6377
                     /gene="pgi"
                     /locus_tag="ACFNMK_001692"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6364913.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            6520..6942
                     /locus_tag="ACFNMK_001693"
     CDS             6520..6942
                     /locus_tag="ACFNMK_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6364914.1"
                     /translation="MALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEE
                     RYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKFSEL
                     ACKTAETEARLEELHNRKKALIDEMAREADKKELPKRL"
BASE COUNT         1883 a         1907 c         1904 g         1507 t
ORIGIN      
        1 aaaaggtatg ccgtctgaaa gtgggaagcg ggtgttatta tagtacaaaa tttgtaattt
       61 tgttacaaaa aaatcaggca ttgaaaagaa atttgattta aatcgtagaa tctttcgatt
      121 gttgtaacgg aataggggaa acctgcccga tttttggact aggtcaaata aattgtaaaa
      181 atgggaatag acaaaaagtg tagcggtgtg tagtattact actcattatg ccacctgcct
      241 ttgtttccga tattgcgatg cagcgtgatg gggaaaacag gtttcaaacg gcatcgaaaa
      301 attctttcct gacgagataa tacgatgagt acacaaacaa attttgattt ggtgttgttc
      361 ggtgcgaccg gcgatttggc gatgcgcaag ctgttgccct gtctgtacca ggcgcatgtt
      421 gccggtctgc ttcatcccga agggcgtatt ttgggggtca gccgcagcga gttggatacc
      481 gaaggttttc tggcgaaggt ggaaaccagt tccaaaatcc atgtgaaaga aaatttttcc
      541 gatgaggctt gggcatcgtt tgtcgaacgt ctcgcatatc tcaaagtcga tgttacgcag
      601 ccggacgatt ttgccgcttt gggcgatttg gtcaaagcgc gcaaagaaac cgacaatgtc
      661 gtcatctatc tttccaccgc gccgaaattc ttcgcgcaaa catgtgaaaa ccttgccaaa
      721 atcggtttga acgccgacaa cgtgcgtgtg gttttggaaa aaccgttggg tacggatttg
      781 gcttcttccc agcaaatcaa taccgatgtt gcgcgttatt tcaaagaagg gcaaatttac
      841 cgcatcgacc actaccttgg caaagaaagc ctgcaaaacc tgcttgcgct gcgttttgcc
      901 aatgtaatgt tcgagccgct gtggaacaat aaatacatcg aaagcgtgca gttgaccatc
      961 gccgaacagc tcggtgtgga agagcgcggc gagttttacg acattaccgg cgcgttgcgc
     1021 gatatggtgc aaaaccattt gatgcagatg ctgtgcatga ctgcgatgga agcccccgcc
     1081 agcttggatg ccgacgcggt gcgcgatgaa aaagtcaaag tcatcaagtc attgaagccg
     1141 ctgaccgtcg agtctgtcaa tgaaaacgtc gtgcgcggac aatataccgc cgccagaggc
     1201 atgaacggct atcttgaaga aatcaacgtt ccgcaagaca gctttaccga aacctacgtc
     1261 gccattaaag ccgaaatcga aaacgaacgc tggaagggcg ttcccttcta cctgcgtacc
     1321 ggcaaacgca tggcgggcaa agtggcggaa atcgttttga acttcaaaga tttgaacagc
     1381 catatttttg aaggcagccg caccgcgccc aaccggctcg ttatcgagtt gcaaccatat
     1441 gaatccgtgc gcctctatac gcagatgaaa accccggggg caggaaataa ggtcgaaacc
     1501 gtgccgctgg caaccgattt gggcaaagca ttggaaggcc gccgcgcgga agcttacgag
     1561 cgcctgctgc tggatgtgat taacggcaaa ctcgctttgt tcaaccgccg cgacgaactc
     1621 gaagccgcgt gggaatatgt gatgccgatt ttggaaaact ggacaaataa caccacgccg
     1681 ccgcacggct acggcgcaca ctcgtggggg cctgaagccg cgcgcgaact attggcgcgc
     1741 gacggacaca agtggcacga agagcagtaa tacaataatg cgttcagacg gcatggggtt
     1801 tgaaatgccg tctgaacgta agtaaagcag tatagtggat taacaaaaat caggacaagg
     1861 cgacgaagcc gcagacagta caaatagtac ggcaaggcga ggcaacgctg tactggttta
     1921 aatttaatcc actataaaaa gcagtcccga tatttggtat caaaagcata aacaactgtt
     1981 cggccgacat attgctcaac ccgtttccat aacggaatat gccgtctgaa ataaaaaaag
     2041 gacacaaata tgtttgtttg gcacgaatac gaaaatgcgg cagaagcggc gcagtctttg
     2101 gctgacgcag tggcggatgc tttgcagggc gcactggacg agaagggcgg tgcggtgttg
     2161 gcagtttccg gcggacgttc gccgattgca tttttcaacg ccctgtcgca aaaagatttg
     2221 gattggaaaa acgtcggcat caccttggca gatgaacgca tcgtgccgac cgtccacgcc
     2281 gacagcaata ccggtttggt gcgcgaatac ctgttgaaga acaaagcgga agcggcaatg
     2341 tggattccta tggtggaaga cggaaaaact gaaaccgaat tacatcccga tgctgttgtc
     2401 gattatgcac tgaaacatta caaacagccc gatgttttgg ttttgggtat gggaaacgac
     2461 gggcatacgg cttcgatttt cccgaaagct ccgcagtttc agacggcaat cgacggttcg
     2521 gcaggtgtcg cgctggtgca taccacgccc gttaccgcgc cgcacgagcg cgtcagtatg
     2581 accttggatg cgattgccca tacggggcat gtgtttttgg cgatacgggg cgaagagaaa
     2641 aaagccgtgt tcgaccaagc cgcacaaggc gaaaaccgcg aatatccgat caacctcgtt
     2701 ttgaaccatc aaggagtgaa ctgccatgtc ttctacgccg aataaacaag ccggatatcc
     2761 ccgactggtc gccgatatcg gcgggacgaa tgcacgcttt gcgctggaaa ccgcgccgcg
     2821 cgtcattgaa aaagccgccg tgcttccgtg taaagactac gatacggtta ccgatgcggt
     2881 gcgtgcctat ctgaatcaaa gcggtgcaac agccgtacgg cacgcggcat ttgccatcgc
     2941 caacccgatt ttgggcgact gggtgcagat gaccaaccac cattgggcat tttccatcga
     3001 aaccacccgt cagactttgg ggctggacac cctcatcctt ttgaacgact ttaccgcgca
     3061 ggcattggcg gtaacgcaga cttcaagcaa agacctgatg caggtaggcg ggcaaaagcc
     3121 tgtcgaattt gcccccaaag ccgttatcgg ccccggtacc ggcctgggcg tgagcggatt
     3181 ggtgcacagc cacgcaggct gggtggcttt ggcgggcgag ggcgggcata ccagtttccc
     3241 gccgtttgac gatatggaag tgctgatttg gcagtacgcc aaaaacaaat acggccatgt
     3301 ttccgccgaa cgctttttga gcggcgcggg cttgagcttg gtttacgagg ctttggctgc
     3361 aaaacagaaa gccaaacccg ccaaactgat gccgtctgaa atcacggaaa aggctttgag
     3421 cggcgcgtcg cctttgtgcc gtcagacttt ggacatcttc tgcgccatgc tcggcacggt
     3481 tgcttccaac ctcgccctga cgctgggcgc gcgcggcggc gtgtacctgt gcggcggcat
     3541 tattccccgc gtgttggaat atttcaaaac ttccccgttc cgcagccgtt tcgagaacaa
     3601 gggcaggttt gaagcatatc ttgccgcgat tcccgtgtat gtcgtcttga gcgagtttcc
     3661 cggaatttcc ggtgcggctg cggctctcga caaccatttg agaaacgttt aaccacagcg
     3721 gctccttgca gcggggctgc attatcgaag ggcatatcat tatgttaagc aaaatcagcg
     3781 aatcactggc aaacctttcc ggtgcggaac gcaaagtcgc cgaatgtgca ttggcggaac
     3841 ccaaatggtt cgtccatgcc gccgttgccg aaattgccga acgtgcgtcc gtcagccagc
     3901 cgaccgtcat ccgattctgc cgcagcttgg gttataaagg gctgcccgag ttcaagctcg
     3961 ccttgtccgc cagcatcggt catgagggta tgccctatgt ccacgaagaa ctcaacgccg
     4021 acgacgatat ggcaagcgtg gtcgagaaag tgttgggcaa tgccgccgcc tcgctgttgg
     4081 gcgaacgccg cttcctgaaa gagtcggagc tggaaaacgc cattgccacg ctgatgcacg
     4141 cccgtcgcgt cgagttttac ggtgtcggca attccggcat tgtggcacag gacgcgcagc
     4201 ataaattttt ccgtttcggc atgtccaccg tcgcctatgt cgatacgcac acgcagctga
     4261 tggcggcatc tgttttgagc gatcaggatg ttttggttgc catttccaac acgggttcgt
     4321 ctatcgaact tttggatgcg gtcagcatcg ccaaagaaaa cggcgcgtct gtcatcgcac
     4381 tgacccgcaa cgattcgcct ctggcgcaac ttgccgactg cgtgttgagc gttgccacgc
     4441 aggaaaatgc cgaactctac acgcccatgg tttcccgcct cttgcagctt gccgtcatcg
     4501 acattctcgc catcggactt gccctgcgct tgggcgatgc tgccagcctg caattgcaga
     4561 aaagcaaaaa aagcatacac aacaagcaca tcgattacga caaagattga ccttcagacg
     4621 gcatcccaca aatgccgtct gaaatgccga acaacggccg tcggcggctt gcggcagttt
     4681 ccagcagcct tttcatccca caacaaaaaa cctcattcag gagcatatag atgaaacact
     4741 ttcacgactt acccgcatgg tcgaaattgt ggaaccactt tgacgacagc aaaacattgc
     4801 atatgcgcga aatgttcgag caagacccgc agcgtgcgga acgctactgg ctgcaagtcg
     4861 gcggactgac gctggactac tccaaaaacc gcatcaacga cgaaaccatg tcgcttttgt
     4921 tcgagcttgc ccgagaagca ggcgtgccgg agcggatgcg gcagatgttc cacggcgaaa
     4981 aaatcaatac caccgaaaac cgcgccgtcc tgcatgtcgc ccttcgcaac cgcaccaatt
     5041 cgccgattgt ggttgacggt gaagatgtga tgcccaaagt caaccgcgtt ttgcaacgta
     5101 tgggcgaatt tgcacacgaa gtccgcagcg gaagctggct gggctatacc aaccaagtca
     5161 ttaccgacgt tgtcaacatc ggcatcggcg gatcggattt gggtccgctg atgatgtgta
     5221 ccgcgctcaa acctttcggt catccgcgcc tcaatatgca cttcgtctcc aacgtggacg
     5281 gctcgcaact gcgcgacgta ttgtccaaag tccaccccga aaccacgctg ttcatcatcg
     5341 cctccaaaac atttaccacg caggaaacgc tgaccaacgc gctgaccgcg cgcgaatggt
     5401 ttttgaatca tgcgggcgac gaagaagccg ttgccaaaca cttcgccgcc gtttccacca
     5461 atcaaaaagc cgtcgccgaa ttcggcatcg acaccgccaa tatgtttgaa ttttgggatt
     5521 gggtcggcgg tcggtacagc ctgtggtccg ccatcggatt gccgattatg ctgtatctcg
     5581 gcgaagaaaa cttcatcgaa atgctcaacg gcgcgcacct gatggaccaa cacttcatca
     5641 acacaccgct cgagcgcaac ctgcccgtca ttctcgccct catcggcatc tggtatatca
     5701 actactacgg cggcggcagc cacgtcatcg cgccttacga ccaacatttg caccgcctgc
     5761 ccaaattcat ccagcagctc gatatggaaa gtaacggcaa acaggttacg ttggacggca
     5821 aagcagtcgg acacgaaacc tcgccgatta tctggggcga aacgggcatt aacggccagc
     5881 acgccttctt ccaactgctg caccaaggca cgcacattac ccccatcgac ctgattgcct
     5941 cacttgaaaa acgcagcaac ctgcccggac accacgaaat cctgcttgcc aacgtctttg
     6001 cacaagcaga agcctttatg cgcggcaaaa cccccgacga agtccgtgcc gaactcaaag
     6061 cgcagggtat ggatgaggcg cgcatcgaag agctggtccc gcacaaaacc ttctccggca
     6121 accgcccgac caacctcatt ctcatggaca aggtcaaccc gcgcaatatg ggcagcctga
     6181 ttgccatgta cgaacacaaa accttcgtac aaggcatcat ttggggcatc aacagcttcg
     6241 accagtgggg cgtggaactc ggcaaacaac tggctaaaac cattttgggc gaactgaccg
     6301 gcgaaaccgg gccgcaaaag cacgacagtt cgaccgaacg cctgatcaac ctctacctgc
     6361 agaccaaccg caaataaaac ctgcggaaaa atgccgtctg aacgccgacc gtttcaaacg
     6421 gcatttttat cgaacaggaa aaccgtcggt aaacttgcag agcgtgtgca atcccgatat
     6481 gatggtttgc ataaatttaa acatatatgt tccgcagcta tggcactgat taaagagccg
     6541 ttggacaaag cgaaacaaag gaacgaagaa cttgaagcgg cagaagaagc ggcggcgcag
     6601 gaggcattgg gtcgggagca ggaagtcgac cgcgtatccg aatgggaaga acgctacaag
     6661 ctgtcgcgca gcgagttcga gcaattctgg aaaggattgc ctcaaaccgt acagaataag
     6721 ctgcaagcct cacagaaaac atggaaaagc gggatggata aaatctgtgc caacaatgcg
     6781 aaagctgaag gtgaaacgcc aaacggcata aaattcagcg aactggcatg caaaacggcg
     6841 gaaaccgaag cacgcttgga agagctgcac aaccgtaaaa aagcccttat cgacgaaatg
     6901 gccagggaag cggacaagaa agaactgcca aagcggctct gaacagcgcg gttcaggcac
     6961 tgcccgccga tattgccgaa accgtcatgc ccgagtaccg caactgacaa aacggtttga
     7021 atgccaaatg cgccgacggc gacgaatacg gcgtggcaca atctgactgc cgtaccagag
     7081 aaatcaatgc gaaaaccaaa gaaatccaag gttatctgat tgactgaaac ttggatgcgg
     7141 gaatgtcggc ggcttttgcg tttttgtcgt ttttatagtg aattaaattt aaaccgatac
     7201 a
//
LOCUS       DAXHTY010000092         7181 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXHTY010000092 DAXHTY010000000
VERSION     DAXHTY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.092"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..1044
                     /locus_tag="ACFNMK_001694"
     CDS             <1..1044
                     /locus_tag="ACFNMK_001694"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6364915.1"
                     /translation="LEGDSGEWDDLVPENVTLIYLKKYTKSSFNQTVYNQILQRLIIQ
                     FEIKILWNFNCRETYNFTERCSPFIREYLSVWGLVFAHWLRPGSLQEFGMAHENLPFV
                     IDDYDVLISDNKTFTEYLCHQYGWNQDKFKTLYVPNSSESIQVKHHRKGNSLKVLWAS
                     GIEWNKGVEILPKIAEKISNLPITIDIYGGTKNKDGEDLLLKLNKDILLKENIKYKGK
                     FDNFRKLVEEGQYDCFLFTSIIEGMPNVIIEAAERQMSIITPLIGGLGEFLNSENAYI
                     VDNKNDVEKYIIELQKVLENKNTGKFTKEINLAHSYNKKFTLDSFKLSYNSLQDYLYT
                     IKDKKNKRIINEN"
     gene            1034..3316
                     /locus_tag="ACFNMK_001695"
     CDS             1034..3316
                     /locus_tag="ACFNMK_001695"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6364916.1"
                     /translation="MKIELINSYKQKKYVEAKYIAKKILLTDAKDYEALFYLANSFLF
                     TGEFKSAKDYFQLIIEYYPDKYQGYEGLYKIYKANGDRYNELIQLKKILENFPNVVHI
                     YKETTLLLLQENLINEAENISLECIKKFPDSYHGYESYAFVLEKKQEWEKALEIWNNI
                     IHKFPNFLAAHLNMLKLHVKIYGYERTTSLFESSSQKFKNTEKIDKLYAIEAEKAFKW
                     NDATEIWSRLLSQYPLNTFFVSRKAIALRHVKGIIASREYLFSALKDQPDNLVLKLNI
                     LDTYLIEKKWEYSLLYGNDIIKNVQDTAQKSNIYFKLRIPYRELKKLDELENIFLDLI
                     KENPSIYISWKGYATVPELMLDGNEKLLKKACHRWKRASEKFPNIVEAKIKYGEILLS
                     MQQLDFANQVFIELKRDFPNNFYAWKGWCLAAHHMRNWVEARKRLLETRIKFPSQFNS
                     IYYYLVNVLQNDNRLAEIENSFIWHTKSLFKNSLNECLELSKINIYRIIGNYKKADEK
                     ALELLNVLPFKDPTTLPKPQKSNISQYIEHSKNNETLVICFSGMDGKRTQNHYRNYGL
                     QCIDKIAFNSSDTYNYQGFANKTIKYNFLLLRDNYNSWYQIHTDYYRTIIENYIKSGN
                     YKKIVLVGASSGGFAAMMYGQLVHADIVYVQAPQTLAFTSYSTQFNKELELQYNLSST
                     AYTSIAKLQNQSNGFIPKVYVQVCENNGIDMFSINMLNTSDPNLFVHKYKNDTHSVHT
                     IIGKSKMFQDICSIIDDLQK"
     gene            3388..4239
                     /gene="kdsA"
                     /locus_tag="ACFNMK_001696"
     CDS             3388..4239
                     /gene="kdsA"
                     /locus_tag="ACFNMK_001696"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC6364917.1"
                     /translation="MPNINLPTFNIKISNSLPFTLFGGINVLEDLDSTLFACEKYVEV
                     TEKLKIPYVFKASFDKANRSSIHSYRGVGLEEGMNIFSAVKDRFGVPVITDVHEPYQC
                     NAVAEVVDILQLPAFLARQTDLVFAMAQTGRVINIKKPQFLSPEQMKNIVEKFKEAGN
                     DQLILCERGANFGYDNLVVDMLGFGVMKKTCDDLPVIFDVTHALQKRDSGSSASGGRR
                     EQVLDLAMAGMATGLAGLFLEAHPNPDKARCDGPSALPLDKLEDFLLRVKAVDETVKS
                     FPKLDIA"
     gene            4239..5528
                     /gene="kdsB"
                     /locus_tag="ACFNMK_001697"
     CDS             4239..5528
                     /gene="kdsB"
                     /locus_tag="ACFNMK_001697"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC6364918.1"
                     /translation="MNTENLKTVIVVPARYASTRLPGKPLADICGKPMIQHVYERACK
                     VPYIDDVIVAVDDRRVAEVVESFGGKVIMTSTQHDSGTDRLVEVMGKYAADIYINIQG
                     DEPLIRSEDIALLAQGMKDEQISVGTLCHALPASEATNPNTVKVVLSANGNALYFSRS
                     PIPYPRDKEHAYYFKHIGVYAYRKETLAKYSNLKQPDIELSEKLEQLRLLDAGIDIRV
                     FEVPETGPGVDTPECLERVRAIMSGQLPIDKHSVNLADIRLVITDVDGVLTDGGIFYN
                     ENGECLKRFHVRDGLGIRLLEESGIKVAVLSGRDSPTLRKRIDDLGISYYQLGIKDKH
                     AACIELMQEANCLKEQTAYIGDDTIDLPAFSACGLSFAVADTPEYIRNQADITLTLPG
                     GYGAFRELADKILAAQGKEDVFSSEAGFAQVMHGANQ"
     gene            5555..6265
                     /locus_tag="ACFNMK_001698"
     CDS             5555..6265
                     /locus_tag="ACFNMK_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC6364919.1"
                     /translation="MKTNFLKLAVETLQLEANAIMEMSKRLDNEFEKAIEIILNTQGR
                     VVVVGMGKSGIIGQKLAATMASTGTSAFFVHPGEAFHGDLGMIKPIDVALLISNSGET
                     EEIIRILPFLKEQGNKIIAMTGNIKSTLAKHAHSLLDISVSREACSNNLAPTSSTTCT
                     LAMGDALAMVLQSEKKFLPEDFARFHPGGSLGRRLLTRVSDVMKTKIPHCLPDASFKE
                     IVYSINRGYMGLTIGNGA"
     gene            6255..6503
                     /locus_tag="ACFNMK_001699"
     CDS             6255..6503
                     /locus_tag="ACFNMK_001699"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFC6364920.1"
                     /translation="MEHDTLHGIITDGDLRRAFDRFDNINQIKAKDIMSLSPKYVSAD
                     ARFAEAEAYMHAEKIDSLVVKESNKVIGVLNIYDIDHY"
     gene            6577..>7181
                     /gene="galE"
                     /locus_tag="ACFNMK_001700"
     CDS             6577..>7181
                     /gene="galE"
                     /locus_tag="ACFNMK_001700"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC6364921.1"
                     /translation="MKVLVTGGTGFIGSHTAISLIKAGYDVVILDNLCNSSINILPRL
                     KEITGKDITFYQGDIRDRALLQKIFAEHKIETVMHFAGLKAVGESNVLPMKYYDYNVS
                     GSLILAEEMAKAGVFSIVFSSSATVYGDPARTPITEDMPVGGTTNPYGTSKYMVERIL
                     SDIQKSDSRWSVILLRYFNPVGAHESGLIGEYPNGIPNNLLP"
BASE COUNT         2464 a         1133 c         1308 g         2276 t
ORIGIN      
        1 ttagaaggag attcaggaga atgggatgat cttgtcccag aaaatgtaac gctcatttat
       61 ttaaaaaaat atacaaaatc ttcatttaat cagactgttt ataatcagat tctacaaaga
      121 ttaataattc aatttgagat taaaatctta tggaatttta attgtaggga aacatataat
      181 tttacagaaa gatgctcccc attcatacgt gaatatttat cggtgtgggg attagttttt
      241 gctcattggc tgcgaccagg aagtttacaa gaattcggca tggctcacga aaatttgcca
      301 tttgtgattg atgactacga tgtattgatt tctgacaata aaacatttac cgagtattta
      361 tgtcaccaat atggctggaa tcaagataaa ttcaaaactt tatatgtgcc caactcttca
      421 gaatctattc aagttaaaca ccatcgtaaa ggtaactcac taaaagtttt atgggcttca
      481 ggtatcgaat ggaataaagg tgtagaaatt cttcctaaaa ttgcagaaaa gatttcaaac
      541 ttaccaatta ctattgatat ttatggaggt actaaaaata aagatggtga agacttatta
      601 ttaaagctta ataaagatat tttattaaag gaaaatatta aatataaagg taaatttgat
      661 aatttcagaa aattagtaga agaaggccag tatgattgtt ttctatttac aagtataatt
      721 gaaggaatgc caaatgttat tatagaagca gctgagagac aaatgtcaat cattactcct
      781 cttattgggg ggcttggtga attcttaaac tcagaaaatg catatattgt tgacaataaa
      841 aatgatgtgg aaaaatatat aatagagtta caaaaagttt tagagaataa aaatactgga
      901 aaatttacaa aagaaattaa cttagctcat agttataaca aaaaatttac cctcgatagt
      961 tttaagttat catataattc tttacaagat tatttgtata ctattaagga taagaaaaat
     1021 aaaaggatta taaatgaaaa ttgaattaat taattcatat aaacagaaaa aatatgtaga
     1081 ggcgaaatat attgctaaaa aaatattact tacggatgca aaagattatg aggccttatt
     1141 ctatcttgca aacagctttt tatttacagg agaattcaaa agcgcaaaag actactttca
     1201 gttgattata gaatattacc ctgataaata ccaaggatac gaaggcttat ataagattta
     1261 taaagccaat ggagataggt ataatgaatt aatccaacta aagaaaattt tagaaaattt
     1321 cccaaacgtt gttcatatat ataaagaaac tactttgctt ctactacaag aaaatttaat
     1381 aaatgaagct gagaatatta gccttgagtg tattaaaaaa tttcctgatt cctaccatgg
     1441 atatgaaagc tatgcttttg tattagaaaa aaagcaagag tgggaaaaag cacttgaaat
     1501 atggaataat atcattcata aatttcctaa tttcttagca gcacatttaa atatgctgaa
     1561 attacacgtg aaaatttatg gctatgaaag gactacctca ctatttgaga gtagttctca
     1621 aaagtttaaa aatactgaga aaatagataa attatatgca attgaagctg aaaaagcctt
     1681 taaatggaat gatgctacag aaatctggtc acgactgctt tctcagtatc ctctaaatac
     1741 cttttttgta tcccgcaaag caatagcact aagacatgta aaaggcataa tagcctctcg
     1801 agaatattta ttttctgcac tcaaggatca gcctgataat ttagtattaa aactaaatat
     1861 attagatact tacttaattg aaaaaaaatg ggaatattct ctattatatg gaaatgacat
     1921 tataaaaaac gtgcaagata ctgcacaaaa atcaaatatt tattttaaac ttcgaatccc
     1981 ttatcgtgaa ttgaaaaaat tagatgaact agaaaacatt tttttagatt taattaaaga
     2041 gaatccgagt atttatatta gctggaaagg ttatgctaca gttccagaat taatgttaga
     2101 tggtaatgaa aaacttctaa aaaaagcttg tcatcggtgg aaaagagcct ctgaaaaatt
     2161 tccaaatatc gttgaggcta aaataaaata tggtgagatt cttttgtcta tgcaacagct
     2221 agattttgct aaccaagttt ttattgaact taaacgagac tttccaaata acttttatgc
     2281 atggaaaggt tggtgcctag ctgctcatca tatgagaaat tgggtagaag ctcgtaaaag
     2341 gcttttagaa actcgtatta aatttccaag tcagtttaat agcatttatt attatttagt
     2401 taatgtatta caaaatgata atagactagc cgaaatagag aactccttta tctggcatac
     2461 taaatctttg tttaaaaact cccttaatga atgtttagaa ctatctaaaa ttaatattta
     2521 caggattata ggaaattata aaaaagctga tgagaaggca ttagagttat taaacgtatt
     2581 accatttaaa gatcctacta ctctgccaaa acctcagaaa tctaatatct ctcaatatat
     2641 tgaacattct aaaaataatg aaactttagt tatatgcttt tcaggcatgg atggtaaacg
     2701 cactcaaaat cattatagaa attatgggtt acaatgtata gataaaattg cttttaattc
     2761 tagtgatact tataattatc aaggttttgc taacaaaacc ataaaatata attttctctt
     2821 actaagagat aattataaca gttggtatca aatacataca gattattata gaacgatcat
     2881 tgaaaactat ataaaatctg gcaattataa aaaaatagta ttagttggag cttcatctgg
     2941 aggctttgca gctatgatgt atggacagtt agtacatgcc gacattgtat atgttcaagc
     3001 tcctcagaca ttagctttca caagctactc aacacaattt aataaagagc tagagcttca
     3061 atataattta agttctactg catatacaag tattgctaag cttcaaaatc aatcaaatgg
     3121 atttattccc aaagtatatg ttcaagtttg tgaaaacaat ggaattgata tgttttctat
     3181 caatatgctt aatacttctg atcctaattt atttgttcac aaatataaaa atgatactca
     3241 ttctgttcat acaattatag gaaaatcaaa aatgtttcaa gacatatgta gtataataga
     3301 tgatttacaa aaataatttc ttattgaata aaaattcaat cattaaatta gatctaaaaa
     3361 atttattttt aatcaaggat tttaaaaatg cctaatatta atttaccaac cttcaatata
     3421 aaaattagta atagcctacc ctttacatta tttggaggaa ttaatgtctt agaagaccta
     3481 gattccactt tatttgcctg tgaaaaatat gttgaagtaa cggaaaaatt aaaaatccca
     3541 tatgtattta aagcatcttt tgataaagcc aatcgctctt caattcattc ttaccgcgga
     3601 gtaggccttg aagaaggcat gaacatcttt tctgcggtta aagaccgttt tggtgtccct
     3661 gtgattacag atgtgcatga gccatatcaa tgtaatgcag ttgctgaagt agtggatatt
     3721 ttgcaattac cggcctttct tgcccgccaa actgatttgg tattcgccat ggctcaaacc
     3781 ggtagagtta tcaacattaa aaaacctcaa tttcttagcc ctgaacagat gaaaaatatt
     3841 gtggagaaat tcaaagaggc tggcaatgac caattgatct tatgtgaacg tggcgctaat
     3901 tttggctatg ataatttggt tgtcgatatg ctgggcttcg gtgtaatgaa gaaaacttgc
     3961 gatgatttac cggttatttt tgatgtaaca catgctcttc aaaaacgtga ttcaggctcc
     4021 tctgcttccg gtgggcgtcg tgagcaagtt ttggatttag caatggccgg catggcaact
     4081 ggtttagcag gtttattctt ggaagctcat ccaaatcctg ataaagcgcg ttgtgatggc
     4141 ccaagtgctc ttcctctaga taaattggag gatttcttgt tacgtgtgaa agctgtggat
     4201 gaaacagtta aatcatttcc taaattggat attgcttaat gaataccgaa aatctgaaaa
     4261 cggttattgt tgttcctgca cgctatgctt ctactcgttt acccggaaag cctttggctg
     4321 atatctgcgg taaaccaatg atccaacatg tctatgaacg tgcttgtaaa gtaccctata
     4381 ttgatgatgt gatcgttgct gttgatgatc gtcgtgttgc tgaagttgta gaatcttttg
     4441 gtggcaaagt tatcatgact tctactcaac atgattctgg tacagatcgg ttagttgaag
     4501 taatgggaaa atatgctgct gatatttata ttaatatcca aggagatgag ccattaattc
     4561 gatctgaaga tattgctttg ctcgctcaag gcatgaagga tgagcaaatc tcagtgggca
     4621 ccctttgtca tgctctacct gcttcagaag caactaatcc aaatactgtc aaagtcgtac
     4681 tatctgcaaa cggtaatgca ctttacttta gtcgtagccc tattccttat cctcgagata
     4741 aagagcatgc ttattatttc aaacatatcg gcgtttatgc ttatcgtaag gaaactctag
     4801 caaaatacag taacctcaag cagcctgata tagagctaag tgagaaactg gaacagttgc
     4861 gtttattaga tgccggcatc gatattcggg tttttgaagt acctgaaaca ggaccgggtg
     4921 tagatacacc agaatgctta gaacgtgtac gcgccatcat gtcaggccag cttcctattg
     4981 ataaacattc ggtcaattta gctgatattc gtctcgttat taccgacgtt gatggtgtac
     5041 taacagatgg cggtattttc tacaatgaaa atggagaatg tttaaaacgt tttcatgtac
     5101 gagatggttt aggtattaga ttgcttgagg aatcaggcat taaagtagcg gtattatcag
     5161 gaagagattc tcccaccctg cgaaaacgaa ttgatgattt aggtatttct tattatcaac
     5221 tcggcattaa agacaaacat gcggcatgta ttgagttaat gcaagaagct aattgtctta
     5281 aagagcaaac agcatatatc ggtgatgaca ccatcgatct acctgctttc tccgcttgtg
     5341 gtttaagttt tgctgtcgca gatacgcctg aatatatacg taatcaagca gacataactt
     5401 taacattacc aggaggctat ggggcatttc gtgagttagc agataaaatt cttgctgctc
     5461 agggcaagga ggacgttttt tcttcagaag ctggatttgc tcaagttatg catggtgcta
     5521 accagtaatc ataaaaaaat aataggagtt aataatgaaa actaactttc taaaattggc
     5581 ggttgaaacc ttacaattag aagctaatgc cattatggaa atgagtaagc gcttagataa
     5641 tgaatttgag aaagcaatag aaattatctt aaatactcaa ggtcgcgtag ttgtggtagg
     5701 gatgggaaaa tctgggatta tcggtcaaaa gcttgctgca actatggctt ctaccggtac
     5761 ttctgctttt tttgtccatc ctggtgaggc atttcatggc gatttaggga tgattaaacc
     5821 aatagacgtt gccttattaa tttcaaattc gggagaaact gaggaaatta tccgaattct
     5881 tccttttctg aaagagcaag gcaataaaat tattgccatg accggtaata ttaaatcaac
     5941 tttagccaaa catgctcatt ctttactgga tatcagtgta tcacgtgagg cttgttcaaa
     6001 taatcttgcg ccaacctcat caacaacctg tacacttgca atgggcgatg cattggcaat
     6061 ggttttacaa tctgaaaaaa aattcttgcc cgaagatttt gctagatttc atcctggtgg
     6121 cagcttaggc cgccgtctgt taacgcgtgt ttcagatgtg atgaaaacca aaattccgca
     6181 ttgcctaccc gatgcttcct ttaaagaaat tgtatatagt attaatcgag gttacatggg
     6241 gctaaccatt ggtaatggag catgatacgc ttcatggcat tattactgat ggagatttac
     6301 gtcgtgcctt cgatagattt gataatatca atcaaattaa agcaaaagac ataatgtctt
     6361 tatctcctaa atatgtttct gctgatgccc gctttgcaga agctgaagcc tatatgcatg
     6421 ctgaaaaaat tgattcatta gtcgtaaaag agtccaataa agtaattggc gttttgaata
     6481 tttacgatat tgatcattat taaaaagcta actgctaatt acgaattatt tcaaatataa
     6541 tgtaattaac tctaaactaa ataaagttaa agaataatga aagtcctcgt tacgggcgga
     6601 accggtttta tcggttcaca taccgccatc tcactaatta aagcaggtta tgatgttgta
     6661 attctcgata atctgtgtaa ttccagcatc aatattctgc cacgtttaaa agaaatcacc
     6721 ggtaaggaca ttacctttta tcaaggcgat atccgtgatc gtgctttgct gcaaaagatt
     6781 tttgctgaac ataagattga aacggtaatg cattttgccg gcttaaaagc agtgggtgaa
     6841 agcaatgtct taccgatgaa gtattatgat tacaatgtat cgggcagcct gattttggcg
     6901 gaagaaatgg ccaaagcagg tgtattcagt atcgtattca gttcgtcagc taccgtttac
     6961 ggcgatccag cacgtacgcc gattacggaa gatatgccgg taggcggtac gaccaaccca
     7021 tacggtacat cgaaatacat ggtggagcgg attctatcgg atattcaaaa atcagattca
     7081 cgctggagtg tgattttact gcgttatttc aatccggttg gcgcacacga aagtggtttg
     7141 attggcgaat accctaacgg tattccaaat aatctgctgc c
//
LOCUS       DAXHTY010000093         7101 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXHTY010000093 DAXHTY010000000
VERSION     DAXHTY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.093"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..429
                     /gene="mafB"
                     /locus_tag="ACFNMK_001701"
     CDS             <1..429
                     /gene="mafB"
                     /locus_tag="ACFNMK_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6364922.1"
                     /translation="NKPPKPSTQQHPTHSDNNIGLPASYVKPDTSISPTGTIQDRIRW
                     TKSKFPTEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSDKILGYQTEHRRVRY
                     DINNNIYVLANPKTFKIKTMFKPNLGKKYYDGEFKKDMGN"
     gene            395..601
                     /locus_tag="ACFNMK_001702"
     CDS             395..601
                     /locus_tag="ACFNMK_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC6364923.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTKVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            622..840
                     /locus_tag="ACFNMK_001703"
     CDS             622..840
                     /locus_tag="ACFNMK_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364924.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNIL"
     gene            837..1196
                     /locus_tag="ACFNMK_001704"
     CDS             837..1196
                     /locus_tag="ACFNMK_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6364925.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            1272..1484
                     /locus_tag="ACFNMK_001705"
     CDS             1272..1484
                     /locus_tag="ACFNMK_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364926.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            1571..1687
                     /locus_tag="ACFNMK_001706"
                     /pseudo
     CDS             1571..1687
                     /locus_tag="ACFNMK_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1746..2102
                     /locus_tag="ACFNMK_001707"
     CDS             1746..2102
                     /locus_tag="ACFNMK_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC6364927.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWGLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            2150..2278
                     /locus_tag="ACFNMK_001708"
     CDS             2150..2278
                     /locus_tag="ACFNMK_001708"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364928.1"
                     /translation="MPQTWGEAIQFRIKKQIENELAPPNWSTQFPNGSIYDPKVTK"
     gene            2275..2649
                     /locus_tag="ACFNMK_001709"
     CDS             2275..2649
                     /locus_tag="ACFNMK_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6364929.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
     gene            complement(2654..2949)
                     /locus_tag="ACFNMK_001710"
                     /pseudo
     CDS             complement(2654..2949)
                     /locus_tag="ACFNMK_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039136685.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3029..3556)
                     /locus_tag="ACFNMK_001711"
                     /pseudo
     CDS             complement(3029..3556)
                     /locus_tag="ACFNMK_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684692.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(3605..4591)
                     /locus_tag="ACFNMK_001712"
     CDS             complement(3605..4591)
                     /locus_tag="ACFNMK_001712"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC6364930.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALLAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKAGVREALQAVR"
     gene            complement(4631..5431)
                     /locus_tag="ACFNMK_001713"
     CDS             complement(4631..5431)
                     /locus_tag="ACFNMK_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC6364931.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAVALDLPVVRHAQAAGFIEG
                     VTLKRGEPLDSLEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYAGRFNQTERGSRSVYVFEQPESRITPIMEHIEQTYPGVRSYSLPSVGWS
                     NADGTAVRPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGVELKNRVN"
     gene            5556..6899
                     /gene="argG"
                     /locus_tag="ACFNMK_001714"
     CDS             5556..6899
                     /gene="argG"
                     /locus_tag="ACFNMK_001714"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC6364932.1"
                     /translation="MSQNHTILQNLPIGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVNVRFEEGVPVTLNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            6974..>7101
                     /locus_tag="ACFNMK_001715"
     CDS             6974..>7101
                     /locus_tag="ACFNMK_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6364933.1"
                     /translation="MKEYKVVIYQESPLSSLFFGAAKVNPVNFSAFLNKQPPEGWRV"
BASE COUNT         2023 a         1597 c         1597 g         1884 t
ORIGIN      
        1 aataagccac caaaaccaag tacgcagcaa caccctacac actctgataa caatatcggc
       61 ttacctgcct catatgttaa acctgataca tctatttctc cgacaggaac aattcaagac
      121 cgcatcagat ggacaaagtc caagtttcct actgagaaat ctttaaatgg acatttcaaa
      181 gctcatggaa aagaatttgg cgatataacc attgaagact accaaaaaat ggcgtctgat
      241 ttgttatcaa aacagacatc ggacaagata ttaggttatc agacggaaca tagacgagtg
      301 cgctatgata tcaataacaa tatctatgtt ttggccaatc caaaaacatt caaaatcaaa
      361 acaatgttta aaccaaactt aggaaagaag tattatgatg gagaattcaa aaaagacatg
      421 ggaaattgac ggagaaatat ggctacattg tcctgtttgc ggaactaaag ttatggacta
      481 tgatatctgt gacgtttgtc agtggcaaaa tacaggagaa actaatatag atggtggccc
      541 taatgaaatg acacttgcgg aggcgaaaga agcttacgca aaaggcttac caatcagata
      601 aataagcacc tagagaaatc aatgatgacg gaatcccatg gttacctatc aaataattta
      661 cctgttaaaa tcataaatga tattatttat gcaacccagt tagtcgaaga tttggtttta
      721 ggaaaaataa aaattgttga ttttttaaaa tcatataaca atttttattg ttggcttggt
      781 tttgatgagt tgcctcaatc tgagaaaata aaattcctaa gctatctcaa tatattatga
      841 gattaaaatt aaaaaattat gattgttctg actttgattt ggaagatttc ccacaagaca
      901 agttagaaaa tttttgtata cttttgacat tatcaatagg ttttgatgaa agtaatggtg
      961 ctgattattt ttatgtttat atatatagca ccgaatggtt actttcaaat atacatagac
     1021 cgatgtcatt aaaaaactca attgttacaa accgttttaa tattgaacac atattaaaat
     1081 tgataaatga tatattggaa atatgtaatt caacgtctga agataagtct atatcaaatt
     1141 tggcaaaata ttttgactgg gaatttgacg attataatct aaatatccaa gactaaagat
     1201 ggtggacagc atttagacca aaaacaggca aacggtattt tgatgacgaa ttaaaataaa
     1261 aggaattatt catgaaatta attaattttg aagagagttc agttaaaatt agtctcgata
     1321 aagatgagtt gtatattatt caagcaattg taggagaaat ttattcaggc gtatgcgtgg
     1381 attgtagaga ttttgagata attcatggtg ttgaaaaaat aaagttcttt ctttggataa
     1441 agaacttaaa aaaatatatg atacttggga taaatgttag ctaggtaagg tgggcacttg
     1501 ttgcccacct tttgccgtat attaaaaatc cttttcaggc tggaactggg tgggcatcaa
     1561 tgccaaccct aattgtgctg atgaagcagg aaaactgata tgggaaaacg accccaataa
     1621 aaattggtgg gctaaccgta tggatgatat tcgcggcatc atccaaggtg cggttaatcc
     1681 tttaatttac aaggtaagca agcaaaagat tatttacaac aaactcatat caggaactta
     1741 gacaaatgaa tgaacacaac ctgttaattt tctgtttaaa agacaatgtt tcaattagtg
     1801 aatatactga aatgattgat tgggcttata aaaacattca atctgaaaca gttgtagaaa
     1861 ttacggaaaa tcaaattatt gaatatcaaa atcgtggatt atgggggctt gtttctgaaa
     1921 ttaccgataa ttggttattt ggaccaagtg agggggattg gctaatagat aaggaaagta
     1981 ttttggctgt aaaagaaaaa ttacaaaatt cagatttttc tacagagccc ttagtgaaaa
     2041 atattattca tgtacttgaa tatgctataa aaaatgaaaa aacagtaatt tttcattttt
     2101 gagactaatc caatttttag taatattgat gcagagcaag cagcattaga tgccgcaaac
     2161 atggggagaa gctattcaat ttagaattaa aaaacaaatt gaaaatgaac tagcaccacc
     2221 aaattggtct acccagtttc ctaatggtag tatttatgat cctaaggtaa cgaaatgatt
     2281 attcaaaatg aatttaattt atatcctagt aatatgcttc ctgaaaggtt ttgttatcct
     2341 gaaaagtatg ttcgtatctc taacgataca tctttaatac cttatattca gccacataat
     2401 tttcactggt ggtttgagaa ttatggaaca gaaggggcag aagtagctta tatatttaga
     2461 aattctatcc tgcctgattt aaatcttatc ccattcgcta gtaatggaga atgggaagct
     2521 tattttgatg gtaatgatgt aacaggaaat cctagggtta ttgtcattaa tttagataat
     2581 atagaaaacc atgaattttt taatagtttt gaagagtggc ttgaattagc aattaaggat
     2641 acttggtaag cagctatcta taaaagagat gggaccatcc tggataacta ggataaactc
     2701 gatttactaa ttgttttaaa atggaaaaag aacttttatc tcactgttgt taaaacgcca
     2761 ttcgcactcc tttaaataca gctcaaaatg cgctttggga atgccgttaa acttgcgtaa
     2821 atgacgtttt gcttgattcc aaaagttctc agttccatta atatggtttt gtcgttcggc
     2881 aaaatgtgtg ctgtgattga tacggaaatg gctaaattcg cccgcatcca atacatcata
     2941 gccacgataa caaaatgagt ttattttgtt tataccggct tagacgactt tttctcatag
     3001 ggataattct aacttaattt gaatttccct agtgatctag ggcagcccct aaattaataa
     3061 agcagcacaa ctccttttgc cgatgttccg gactgtcaaa cgactgttcc tcatgccaca
     3121 tctccatcaa ggtacggata acccgctccg ccttaccgtt ggtctgcgga caagcaaatc
     3181 gggcaagcct ccaaccaatc ccattatcat aacaagctgc accgaaagca tgttggacgg
     3241 ctctttatat tacctatcat tgtcagagta aacgtactca atcaggtaca agcaggggtc
     3301 ggacagatgt tcggtcagaa acttggcagc actgtctgcg gttttgtccg gcaaaatggc
     3361 agagtataaa aatcgtcaat agcgacaaac aggtaatctc gtttatcggc ggccttctgt
     3421 cctttgagca acaacaaccg atcggtatca ggatgaacaa aacctcccgg gacaacctgc
     3481 cttttacggc tttaagtgca cggtaaatag tgacgcggct gacttagtgg cagcatactg
     3541 gggaggtgag tgtttttgtg tatattttta ttttggtatt cccttagaaa tactgtaaac
     3601 aacgctaccg gacggcctgc agggcttcgc gcacgccggc tttgagttct gcgccgaagc
     3661 gtctgcccaa gattctgccg aaatcgtcct tcggagtgta atccaccaca tcgggggctt
     3721 tgaccacgtc tcgcgccacg ctgtaaatat tgccgagtcc gtccaccagc ccgactttca
     3781 gcgcatccgc gcctgtgtac acgcgaccgc tgaacacgtc gggatattgt cggaatttga
     3841 ggcggccgcc gcgtccggtt ttgacggctt tgatgaactc gccgtgtatg ccggtcagca
     3901 tttcttccca gatttttgac tgttcgggcg tttcgggcga aaacggatcg cccatgcctt
     3961 tgttgctgcc tgcaatttta accctgcgtt tcacgccgat tttttccatc aggccggtcg
     4021 cgtcgaaact gctgccgata acgccgatgc tgccgacgat gctggacggg tcggcataga
     4081 ttttgtccgc cgccgccgcg atgtagtagc agccggacgc gcacatatct tccgccacga
     4141 gataaacggg aatgccgggg tgctgcgcct tcagacggcg tatttcttca aaagcggtgt
     4201 tggagacgac gggcgaaccg ccggggctgt tggcgcggat gacgatggct tttgcctgcg
     4261 ggtttttgta ggcggcctcc ataccgtctt tgagtttttt gacctggtct tctacaccgt
     4321 tgccgatttc gccgtacagg ttgacgactg cggtatgcgg cgtgttgccc gccaactgca
     4381 atgcggcttc gtcttttcgg aaaataccgg caagcagggc aaccagaatc agggtgctga
     4441 cggcgcgcca gatgtttttc cacatccgct ccctgcgcct gtcccgatag gcggacagca
     4501 gcacttcgcg catgatgtcg cgctcccata aggtttcccc cgcatttttt gcttcgggtg
     4561 cttcgttttc tcttctgatt cggtattgca tggttttcct taaatatcgt ccgatttggg
     4621 caaacggttt tcagtttacc cgatttttca gctctactcc caatccgtcc aagctgtgca
     4681 acacttccgc ccacgccgcg tccaaaaggt tgacggcttc tccttcggct ttgatgccga
     4741 actcgatatg cggcctgact gccgttccgt ccgcattgct ccagccgacg ctgggcaggc
     4801 tgtaagaacg cacgccggga taagtttgct cgatatgctc cataatcggc gtaatgcgcg
     4861 attcgggctg ctcgaacaca tacacgctgc ggctgccgcg ttcggtttgg ttgaagcggc
     4921 cggcgtaata agtatccaaa acccattccg ccatcgggtg cgccatcacg ggaaagccgg
     4981 ggaagaaata atgctcgcgg atggaaaatc cggcgatgtt gttaaacgga ttgggcacca
     5041 attccgcgcc ttcgggaaaa tctgccattt tcagacgttg ggcgtgttcc agcgaatcaa
     5101 gcggctcgcc gcgtttcagg gttacgcctt cgataaaccc ggcggcttgc gcgtggcgga
     5161 cgacgggcaa atccaaagca accgctgcgg cttgacgcgt gtggtcgtcg ggcgtggcgc
     5221 cgatgccgcc ggtaacaaac gtcggtatgc cgtctgaaaa gctgcggcgc agttgcctga
     5281 ccagcaaatc gggttcgtcg ggcagatatt gcacctgatt gagcttcagc cctttggatt
     5341 cgagcaggga tttgaaaaag gcgaaatgct tgtcttggcg gctgccgtgt aagatttcgt
     5401 cgccgatgat gatgaggttg aacgcattca taatgttttc ggttttaaat accgcctgaa
     5461 acggcggaat acccgttcag acggcatgat ggctgacaat atttcctatt tataagataa
     5521 tggctttgat ttttcaagct atcgaggatt ttttcatgag ccaaaaccat actattttac
     5581 aaaatctccc catcggtcaa aaagtcggca tcgccttctc cggcggactt gatacctccg
     5641 ccgcgctgtt gtggatgaaa ctcaaaggcg cactgcctta tgcctacact gccaacctcg
     5701 gtcagcccga tgaagacgac tacaacgcca ttcccaaaaa agcgatggaa tacggtgcgg
     5761 aaaacgcccg cttaatcgac tgccgcgcgc agttggcaca cgaaggcatc gccgccatcc
     5821 aatgcggcgc gttccacgtt tccaccggcg gcatcgccta tttcaacacc acgcctctgg
     5881 gccgcgccgt aaccggcact atgcttgttt ccgcaatgaa agaagacgac gtaaacatct
     5941 ggggcgacgg cagcacctac aaaggcaacg acatcgagcg tttctaccgc tacggtttgc
     6001 tcaccaatcc cgcgctgaaa atctacaaac cctggctcga ccagcaattt atcgacgaac
     6061 tcggcggccg ccacgaaatg agcgaatttc tgattgccaa cggcttcaac tacaaaatgt
     6121 cggttgaaaa agcctactcc accgattcca atatgctcgg cgcgacccac gaagccaaag
     6181 acttggaatt tttgaactcg ggcatcaaaa tcgtcaaacc cattatgggc gttgcctttt
     6241 gggacgaaaa cgttgaagtc agccccgaag aagtcaacgt gcgctttgaa gaaggcgtgc
     6301 ccgtcacatt gaacggcaaa gaatacgccg accccgtcga actcttcctc gaagccaacc
     6361 gcatcggcgg ccgccacggc ttgggcatga gcgaccaaat cgaaaaccgc atcatcgaag
     6421 ccaaatcgcg cggcatctac gaagccccgg gaatggcgtt gttccacatc gcctacgaac
     6481 gcttggtaac cggcatccac aacgaggaca ccatcgaaca ataccgcatc aacggcctgc
     6541 gcctcggacg tttgctctac caaggccgct ggttcgacag ccaagccctg atgttgcgcg
     6601 aaaccgccca acgctgggtc gccaaagccg ttaccggcga agttaccctc gaactgcggc
     6661 gcggcaacga ctactcgatt ctgaacaccg aatcgcccaa tctgacctac caacctgaac
     6721 gcctgagtat ggaaaaagtc gaagacgctg cgttcactcc gctcgaccgc atcggacagc
     6781 tcacgatgcg caacctcgac atcaccgaca cccgcgccaa actgggtatt tattcgcaaa
     6841 gcggtttgct ctcgctgggc gaaggttcgg tattgccgca gttgggcaat aagcaataag
     6901 gtttgctgtt ttgcatcatt agcaacttaa ggggtcgtct gaaaagatga tcccttatgt
     6961 taaaaggaat cctatgaaag aatacaaagt agtcatttat caggaaagcc cgttgtccag
     7021 cctgtttttc ggcgcggcaa aggtcaaccc cgtcaatttc agcgcgttcc tcaacaaaca
     7081 accccccgaa ggctggcggg t
//
LOCUS       DAXHTY010000094         6712 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXHTY010000094 DAXHTY010000000
VERSION     DAXHTY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.094"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            531..902
                     /locus_tag="ACFNMK_001716"
     CDS             531..902
                     /locus_tag="ACFNMK_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364934.1"
                     /translation="MKKIRLLLEFHCFPVWFYDENDEIIENDWCEELLPQEILSDNHL
                     KELCIWLADEFDKLYCDEMGNFDYEKLQDEFSFQTKENEQEFIDKLTEFRTKFTQKCF
                     GFCALQDNLPKIENGKIVSWQ"
     gene            complement(1037..1621)
                     /gene="rnhB"
                     /locus_tag="ACFNMK_001717"
     CDS             complement(1037..1621)
                     /gene="rnhB"
                     /locus_tag="ACFNMK_001717"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC6364935.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALEKYGVLPEHRRDFAPVRNLLAQQTLF"
     gene            complement(1690..3585)
                     /gene="mnmG"
                     /locus_tag="ACFNMK_001718"
     CDS             complement(1690..3585)
                     /gene="mnmG"
                     /locus_tag="ACFNMK_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC6364936.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLEHYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGSAEMHPHQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLNR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLAAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3910..5334)
                     /locus_tag="ACFNMK_001719"
     CDS             complement(3910..5334)
                     /locus_tag="ACFNMK_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6364937.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYGD
                     LSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITAV
                     FRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKLK
                     MGKDPEFKLSEHPGLKRRIKSDVW"
     gene            5494..6483
                     /gene="pdxA"
                     /locus_tag="ACFNMK_001720"
     CDS             5494..6483
                     /gene="pdxA"
                     /locus_tag="ACFNMK_001720"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC6364938.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGRLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKDLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            6486..>6712
                     /locus_tag="ACFNMK_001721"
     CDS             6486..>6712
                     /locus_tag="ACFNMK_001721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364939.1"
                     /translation="MIKDPSFPRRRESGLFGFSCFWVSGNFQIVIPAQAGIQTFGRRQ
                     YSKIIRKFEVLDSRFHGNDGMLRESGLFGFG"
BASE COUNT         1487 a         1682 c         1900 g         1643 t
ORIGIN      
        1 tcattcccgc aaaaacaaaa aatcaaaaca gaaaactgaa atatcgtcat tcccgcgcag
       61 gcgggaatct aggtctgtcg gtacggaaac ttatcgggaa aaacggtttt tccaaccctg
      121 agactccgga ttcctgtttt cgcgggaatc cggttttttg agtttcagtc atttttgata
      181 aattcttgca gctttgagtt tctagattcc cgcctgcgcg ggaatgacga aatttcagtt
      241 tttgattttt tgtttttgtg ggaatgacgg tttggaagtt tcccgaaatt caaaaaaaaa
      301 cggaaaccgg acggactgga ttcccgtttt cacgggaatg acggatttta ggtttcttta
      361 tctgaaattc ctaattattt gatgactgcg gttaaaaatg gacaaattgt aggaatgcaa
      421 cgcacaagac ctatatatga attcacctat aacggcacaa aacaccgtgt ggctattgat
      481 ataggcaata atgggtttat tgttggtgcc aaccccaaaa gagtagttaa atgaaaaaaa
      541 taagattgtt acttgaattt cactgttttc cagtatggtt ttatgatgaa aatgatgaaa
      601 ttattgaaaa cgattggtgt gaagaattat taccacagga aattttatca gacaatcatc
      661 tgaaagaatt gtgcatttgg ctagcagatg aatttgataa attatattgt gatgaaatgg
      721 gtaatttcga ttatgaaaag ttacaagatg aattttcgtt tcaaacaaag gaaaatgaac
      781 aagagttcat tgataaatta acagaattca ggacgaaatt cacacaaaaa tgttttggtt
      841 tttgtgcgtt acaagataat ttaccaaaaa ttgaaaacgg taaaattgtt tcttggcagt
      901 gagcgtagcg tacgcaactt gttgcccacg cttttgattt tctgtttttc gcaggaatga
      961 cggtttgggt tctttctctt tgaagttgcg atgctgaaaa tgccgtctga agttcagacg
     1021 gcatttttgt gccggtttaa aacagcgtct gctgcgcgag caggtttctg acgggggcga
     1081 agtcgcggcg gtgttcgggc agcacgccgt atttttcgag ggcttccaaa tgctgcttcg
     1141 tgccgtaacc tttgtgtttg tcgaaaccgt attggggatg gcgttgcgcc agtgcgtaca
     1201 tttccgcatc gcgggcggtc tttgccaaaa cggatgcggc ggatatttcg atgattttgc
     1261 tgtcgccttt gacgacggct tcggcaggga tgttcaaatg ttcgggaatg cggttgccgt
     1321 cgatgaatat tttttcggga cgcacagcca aaccgtcaac ggcgcgtttc atcgcgagca
     1381 tggtggcgtg caggatgttg aggctggcga tttcttcggg cgtggcggcg gcgacgtgcc
     1441 acgcggccgc ctgatttttt atcatttcgg caagcgcgtc gcgttttttc tcgctgagtt
     1501 ttttggagtc ggtcagtccg ggcaggtcga atgtttccgg aaggatgacg gcggcggcaa
     1561 acacgctgcc gactaaaggc ccgcgtcctg cctcgtccac gccggcggtc agtatgtgca
     1621 tgatgtttcc tgttgggatg gtgggaatgc cgtctgaacg atgtttcaga cggcatcgta
     1681 ccgatgtgtt tatttcgcgt ctttaaaccc gcgcttcaaa tgcaccatca gcagtgcgac
     1741 ggcggcaggc gttacgccgg aaatgcggct tgcctgcccg acggtttcag gtttgtgctg
     1801 gttgagcttt tgctgcactt ctgccgataa gcctttgact ttgccgtaat cgatgccgtc
     1861 gggcagtttt aaggtttcga tgtcgcggcg gctgtcgatt tcttcgtttt ggcggtcgat
     1921 atagccttgg tatttgactt ggatttcgac ttgctcgacc acttcggcag cgaggcgttc
     1981 agacggcatc gcgccttcga gcgtcatcag cgcggcgtaa tcgaggttcg ggcggcgcag
     2041 gaggtcgtgc aggttggctt cgcggttgag tttttgtccg aatacgcgga tttgttcgtc
     2101 ttcggcgagt ttttgcggcg tgtaccacgt tgttttcaaa cgttggattt cgcgctcgat
     2161 ggcttcgcgt ttttcgttga acatgcgcca ttgcgcttca cccaccaagc cgattttgta
     2221 gccgtcttcg gtcaggcgca tatcggcgtt gtcttccctg agttgcaatc ggtattcggc
     2281 acggctggtg aacattcggt agggttcgtt cacgcctttg gtgatgaggt cgtccaccaa
     2341 tacgccgaga taggcttgtt cgcggcgcag caggagcgga tcttgtccgc ggacgtattg
     2401 cacggcgttc gcgcctgcga gcaggccttg cgcggcggct tcttcgtagc ctgtcgtgcc
     2461 gttgatttgc ccggcgaaaa acagtccctc aatggttttg gtttcaaggc tggctttgag
     2521 gttgcgcgga tcgaagtagt cgtattcgat ggcgtaaccg gggcgcagga tatgggcgtt
     2581 ttccaaacct ttcatactgc ggacgagcgc gatttgaatg tcgaacggca ggctggtgga
     2641 gataccgttg gggtagtatt cgtgcgtggt cagaccttcg ggttcgagga aaatctggtg
     2701 gctgtctttg tcggcgaagc ggttgatttt gtcttcgata gacggacaat aacgcgggcc
     2761 cacgccttcg attttgccgg taaacatcgg gctgcggtcg aagcctgagc ggatgatgtc
     2821 gtgggtttgc gtgttggtat gcgtaatcca gcaggacact tggtgcgggt gcatttcggc
     2881 actgccgcgc acggacatga cgggaacggg tgtgtcgccg ggctgttcgg tcagttggga
     2941 aaagtcaatc gtgcgtccgt cgatgcgcgg cggcgtgccg gttttcagac ggccttgcgg
     3001 cagcttcaat tcgcgcaaac gtccgcccag cgatttggcg gcggggtcgc cggcgcgtcc
     3061 gccttcgtag tgttccaaac cgatgtggat tttgccggac aaaaacgtgc ctgcggtcaa
     3121 cacgacggcg cgtgctttaa actccacgcc catcgcggta attacgccgc tgatgcgttc
     3181 gccgtcgagc gttacgtctt cgacggcttg ttggaaaagg tcgaggtttt cttggttttc
     3241 caacatttcg cggatggcgg ctttatacaa aatgcggtcg gcctgtgcgc gcgtggctcg
     3301 cactgccgcg cctttgctgg cgttcaggcg gcggaattgg atacctgatt tgtcggttgc
     3361 caacgccatt gcgccaccga gcgcgtcgag ttcgcgtacc aaatgccctt tgccgatgcc
     3421 gccgatggag gggttgcacg acatttgtcc gagcgtttca atattgtgtg tgagcaaaag
     3481 cgtctgcgcg cccatacggg cggcggcgag cgcggcttct gtgccggcgt gtccgccgcc
     3541 gacgacgata acgtcgtagg ttttggggta aatcatgtgg gtcatgtgtg tattgcctga
     3601 cggtgtttca gacggcattt atagtggatt aacaaaaacc agtacagcgt tgcctcgcct
     3661 tagctcaaag agaacgattc tctaaggtgc tgaagtacca agtgaatcgg tttcgtactg
     3721 cttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaaaccac tatattcaat
     3781 atgccgtctg aaaaacgaaa tggattcaaa agtaaagggt tgggattgta cgcttgttcg
     3841 ccctgttttt acagtgtgcg gaaagggaaa agccgcttcg cggggaagcg gctccggtaa
     3901 gggcgggatt taccaaacgt cggatttgat acggcgtttc aggcccggat gttcggaaag
     3961 tttgaactcg gggtctttgc ccattttcag cttggcggtg taatcgcgca gcagcataaa
     4021 cgccaagggc gagagcagca ggatggcgac aaggttgatc cacgccataa tgcccatcgc
     4081 catatccgcc atatcccaga ccaaaggcac attggcaacc gcgccgaaat agacccacgc
     4141 caaaaccagc atacggaaaa cggcggtaat cagccaatgg cttttgatga attggacgtt
     4201 ggactcggca taggcatagt tgccgataac ggtggaaaag gcaaacataa acaggatgac
     4261 ggcgaggaag cccgcgcccc attgccccac ttggctgaca atcgccgcct gcgtcaacgc
     4321 cgcaccgctc aaatcgccgt aaggctgttg gtaaatcaag atgatgaagg cggtgcaaga
     4381 acaaacgatg atggtatcga caaacacgcc cagcatttga atcatacctt gcgaaacagg
     4441 gtgtttcact tcggcggcgg cggcgttcgg cgcggaaccc atacccgcct cgttggaata
     4501 caggccgcgt ttgatgccca tcatcatcgt ttgcgaaatc agaccgccga gtaagccgcc
     4561 tgctgccgcg tcgaatttga acgcgcccga aaaaatctga ccgaacacgt ccggaatcat
     4621 cggaatattg gtcaaaatga tgaaaagagc gataaagagg tacaaaaccg ccatcagggg
     4681 gacgacgatt tccgccgctt tagatatgcg cctgatgccg ccgaagataa tcggcgcggt
     4741 taaaatcacc agggcgacgc cgacataatg aggctcccaa ccccacgccg ctttgacggt
     4801 atcggcaatg gtattggtct gaaccgcttc aaacacaaag ccgaaacaga aaatcaggct
     4861 cagggcgaac aacacgccca gccatttctg ccccagccct tgagtgatgt agtaggcagg
     4921 gccgccccgg aaatggtggt tgtcgtagtc gcggacttta aagagctgcg ccagcgaaga
     4981 ttcgacaaac gccgaactca taccgattaa ggcggttacc cacatccaaa acaccgcgcc
     5041 cggtccgccg actttgatgg cgatggccac gcccgcgata ttgcccacgc ccacgcggct
     5101 ggcaaggccg gttacaaatg cctgaaacgg cgtgatgccg tgagggtcgt ccccctgttt
     5161 gcggccgccg agcatttctt tgatgctgcg cccgaacagg cggaattgga caaagcccgt
     5221 ggttacggtg aagaaaagcc ccgtacccaa aagcatataa accaagtatg accacatcgg
     5281 atcgttgatg gcgccgaccc agccgtgcag ccattcggta aagttctcgt tcatatcgct
     5341 tccttaaagt tgaaacccgc acatattggc ggtatgcaag cagggtttaa attttgtaaa
     5401 cgcccattct agcagattgt caacaaaatc agaaaaattt acatcgccgc gcggctgcgg
     5461 cgttagaatc gcattttgtt tggagcaaac acgatgaaac agcctgtttt tgccgttact
     5521 tccggcgagc ctgccggcat cggccccgat atttgtttgg acttggcgtt tgcacgcctg
     5581 ccctgccgct gcgcggtatt gggcgacaaa cacctgttgc gcgcgcgcgc cgaagccttg
     5641 ggcaaaagcg tcgtcttgcg cgacttcgat ccggaatcag gcggcgcggc atacggcgag
     5701 ctggaagtgc tgcacatccc tgccgtcgaa gcggttgagg cagggcggct caatcccgcc
     5761 aacgccgcct atgtgctgca acttttggac accgcgctcg caggcatttc agacggcatt
     5821 ttcgacggca tcgttaccgc gccgctgcac aaaggcatca tcaacgacgc gcgcgcaagc
     5881 acaggttttt tcagcggaca caccgaatat ctggcggaaa aaagcggcac ggggcaggtt
     5941 gtgatgatgc ttgccggcaa agacctgcgc gtcgccctcg taacgaccca cctgccgctg
     6001 aaagacgttg ccgccgccat cacgcaaccg ctgattgaaa gcgtcgcacg cattctgcat
     6061 cacgacttaa aacacaaatt cggcatcaaa aatcccaaaa tcctggtcgc cggactcaac
     6121 ccccacgccg gcgagggcgg acacctcgga cacgaagaaa ccgacaccat tatccccgca
     6181 ttggaaaacc tgcgccgcga agggataaac ctcgccggcc cgtatccggc agatacgctg
     6241 ttccaaccgt ttatgctcga aggcgcggat gccgtattgg cgatgtacca cgaccaaggg
     6301 ctgcccgtgt tgaaatacca cagcttcgga cagggcgtga acatcacgct cggcctgccc
     6361 tttatccgca cctccgtcga tcacggcacc gcgctcgatt tggcggcaac cggcagggcg
     6421 gattccggca gcctgataac tgccgtggag accgccgtcg agatggcgcg cggcagcctt
     6481 taaagatgat aaaagacccg tcatttccgc gcaggcggga atccggtctg ttcggtttca
     6541 gttgtttttg ggtttcgggt aatttccaaa tcgtcattcc cgcgcaggcg ggaatccaga
     6601 ccttcgggcg acggcaatat tcaaagatta tccgaaagtt tgaggttcta gattcccgtt
     6661 ttcacgggaa tgacggaatg ttgcgggaat ccggcttgtt cggtttcggt tt
//
LOCUS       DAXHTY010000095         6642 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXHTY010000095 DAXHTY010000000
VERSION     DAXHTY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.095"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            335..2164
                     /locus_tag="ACFNMK_001722"
     CDS             335..2164
                     /locus_tag="ACFNMK_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC6364940.1"
                     /translation="MMIKPTALLLPALFFFPHTYAPAADLSENKAAGFALFKNKSPDT
                     ESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAPDNVKTM
                     LHSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEYYRNALE
                     NWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADLNVVVDS
                     GRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHYSGASVQ
                     ADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKGYIGSVV
                     WDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGVWYVRDRAGID
                     ARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNALHPENGHYLDGKIGTTL
                     GTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLMFRSGGA
                     SSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDAAANFKR
                     MKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            2219..6388
                     /locus_tag="ACFNMK_001723"
     CDS             2219..6388
                     /locus_tag="ACFNMK_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC6364941.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFKTGKISVGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSNSFSTSPLGGKLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVIVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
BASE COUNT         1572 a         2128 c         1738 g         1204 t
ORIGIN      
        1 ttgcgttgat ttgccctatg cagtgccgga caggctttgc tttatcattc ggcgcaacgg
       61 tttaatttat tgaacgaaaa taaacttatt taatcctgcc tattttccgg cactattccg
      121 aaacgcagcc tgttttccat atgcggattg gaaacaaaat accttaaaac aagcagatac
      181 atttccggag ggccgcaacc tccgaaatac cggcggcagt atgccgtctg aagcgtcccg
      241 ccccgtccga acaacacaaa aacggccgtt cgaaaccctg tccgaatagt gttagaatcg
      301 aaatctgcca caccgagtgc acgacacccg taccatgatg attaaaccga ccgccctgct
      361 cctgccggct ttatttttct ttccgcacac atacgcgcct gccgccgacc tttccgaaaa
      421 caaggcggcg ggtttcgcat tgttcaaaaa caaaagcccc gacaccgaat cagttaaatt
      481 aaaacccaaa ttccccgtcc gcatcgacac gcaggatagt gaaatcaaag atatggtcga
      541 agaacacctg ccgctcatca cgcagcagca ggaagaagta ttggacaagg aacagacggg
      601 cttcctcgcc gaagaagcac cggacaacgt taaaacaatg ctccacagca aaggctattt
      661 cagcagcaaa gtcagcctga cggaaaaaga cggagcttat acggtacaca tcacaccggg
      721 cccgcgcacc aaaatcgcca acgtcggcgt cgccatcctc ggcgacatcc tttcagacgg
      781 caacctcgcc gaatactacc gcaacgcgct ggaaaactgg cagcagccgg taggcagtga
      841 tttcgatcag gacagttggg aaaacagcaa aacttccgtc ctcggcgcgg taacgcgcaa
      901 aggctacccg cttgccaagc tcggcaacac ccgggcggcc gtcaaccccg ataccgccac
      961 cgccgatttg aacgtcgtcg tggacagcgg ccgccccatc gccttcggcg actttgaaat
     1021 caccggcaca cagcgttacc ccgaacaaat cgtctccggc ctggcgcgct tccaaccggg
     1081 cacgccctac gacctcgacc tgctgctcga cttccaacag gcgctcgaac aaaacgggca
     1141 ttattccggc gcgtccgtac aggccgactt cgaccgcctc caaggcgacc gcgtccccgt
     1201 caaagtcagc gtaaccgagg tcaaacgcca caaactcgaa accggcatcc gcctcgattc
     1261 ggaatacggt ttgggcggca aaatcgccta cgactattac aacctcttca acaaaggcta
     1321 tatcggttcg gtcgtctggg atatggacaa atacgaaacc acgcttgccg ccggcatcag
     1381 ccagccgcgc aactatcggg gcaactactg gacaagcaac gtttcctaca accgttcgac
     1441 cacccaaaac ctcgaaaaac gcgccttctc cggcggcgtc tggtatgtgc gcgaccgcgc
     1501 gggcattgat gccaggctgg gggcggagtt tctcgcagaa ggccggaaaa tccccggctc
     1561 ggatgtcgat ttgggcaaca gccacgccac gatgctgacc gcctcttgga aacgccagct
     1621 gctcaacaac gcgttgcacc ccgaaaacgg ccattacctc gacggcaaaa tcggtacgac
     1681 tttgggcaca ttcctgtcct ccaccgcgct gatccgcacc tctgcccgcg caggttattt
     1741 cttcacgccc gaaaacaaga aactcggcac gttcatcata cgcggacaag cgggttacac
     1801 cgttgcccgc gacaatgccg acgttccttc gggactgatg ttccgcagcg gcggcgcgtc
     1861 ttccgtgcgc ggttacgaac tcgacagcat cggacttgcc ggcccgaacg gatcggtcct
     1921 acccgaacgc gccctcttgg tgggcagcct ggaataccaa ctaccgttta cgcgcaccct
     1981 ttccggcgcg gtgttccacg atatgggcga tgccgccgcc aatttcaaac gtatgaagct
     2041 gaaacacggt tcgggactgg gcgtacgctg gttcagccca ctcgcgccgt tttccttcga
     2101 catcgcctac gggcacagcg acaagaaaat ccgctggcac atcagcctgg gaacgcgctt
     2161 ctaaaccgat acggccgctt cagacggcat tgcagcaaac tattttgaaa cagacattat
     2221 gaccgatacc gcaccgacag ataccgatcc gaccgaaaac ggcacgcgca aaatgccgtc
     2281 tgaacaccgc cccaccccgc cggcaaaaaa acgccgcccg ctgctgaagc tgtcggcggc
     2341 actgctgtct gtcctgattt tggcagtatg tttcctcggc tggctcgccg gtacggaagc
     2401 gggtttgcgc ttcgggctgt accaaatccc gtcttggttc ggcgtaaaca tttcctccca
     2461 aaatctcaaa ggcacgctgc tcgacggctt cgacggcgac aactggtcga tagaaaccga
     2521 gggggcagac cttaaaatca gccgcttccg cttcgcgtgg aaaccgtccg aactgatgcg
     2581 ccgcagcctg cacatcaccg aaatttccgc cggcgacatc gccatcgtta ccaaaccgac
     2641 tccgcctaaa gaagaacgcc ctccgctcag ccttcccgac agcatagacc tgcctgccgc
     2701 cgtctatctc gaccgcttca agacgggcaa aatcagcgtc ggcaaagcct ttgacaaaca
     2761 aaccgtctat ctcgaacggc tggatgcttc ataccgttac gaccgcaaag gacaccgcct
     2821 tgacctgaag gccgccgaca cgccgtggag cagttcgtcg ggggcggcct cggtcggctt
     2881 gaaaaaaccg tttgccctcg ataccgccat ttacaccaaa ggcggactcg aaggcaaaac
     2941 catacacagt acggctcggc tgagcggcag cctgaaggat gtgcgcgccg aactggcgat
     3001 cgacggcggc aatatccgcc tctcgggaaa atccgtcatc cacccgtttg ccgaatcatt
     3061 ggataaaaca ttggaagaag tactggtcaa agggttcaac atcaatccgt ccgccttcgt
     3121 gccttccctg cccgatgccg ggctgaattt cgacctgacc gccatcccgt cgttttcaga
     3181 cggcatcgcg ctggaaggct cgctcgattt ggaaaacact aaagccggtt ttgccgaccg
     3241 caacggcatc cccgtccgtc aggttttagg cagctttgtc atccggcagg acggcacggt
     3301 gcatatcggc aatacgtccg ccgccctgct cggacggggc ggcatcaggc tgtcgggcaa
     3361 aatcgacacc gaaaaagaca tcctcgattt aaatataggc atcaactccg tcggcgcgga
     3421 agacgtactg caaaccgcgt tcaaaggcag gttggacggc agcatcggca tcggtggcac
     3481 gaccgcctcg cccaaaatct cttggcaact cggcatcggc acggcgcgca cggacggcag
     3541 cctcgccatt gcaagcgacc ccgcaaacgg acagcggaaa ctggtgctcg acaccgtcaa
     3601 catcgccgcc gggcaaggca gcctgaccgc gcaaggctat ctcgagctgt ttaaagaccg
     3661 cctgctcaag ctggacatcc gttcccgcgc attcgaccct tcgcgcatcg atccgcaact
     3721 tccggcaggc aatatcaacg gctcaataaa ccttgccggc gaactggcaa aagagaaatt
     3781 cacaggcaaa atgcggtttt tacccggcac gttcaacggc gtaccgattg ccggcagtgc
     3841 cgacattgtt tacgagtccc gccaccttcc gcgtgccgcc gtcgatttgc ggctggggcg
     3901 gaacatcatc aaaacagacg gcggcttcgg caaaaaaggc gaccggctta acctcaatat
     3961 caccgcaccc gatttatccc gtttcggttt cggactcgcg gggtctttaa atgtacgcgg
     4021 acacctttcc ggcgatttgg acggcggcat ccgaaccttt gaaaccgacc tttccggcgc
     4081 ggcgcgcaac ctgcacatcg gcaaggcggc agacatccgt tcgctcgatt tcacgctcaa
     4141 aggttcgccc gacacaagcc gcccgatacg cgccgacatc aaaggcagcc gcctttcgct
     4201 gtcgggcgga gcggcggttg tcgataccgc cgacctgatg ctggacggca cgggcgtgca
     4261 gcaccgcatc cgcacacacg ccgccatgac gctggatggc aaaccgttca aattcgattt
     4321 ggacgcttca ggcggcatca acagggaact tacccgatgg aaaggcagca tcggcatcct
     4381 cgacatcggc ggcgcattca acctcaagct gcaaaaccgt atgacgctcg aagccggtgc
     4441 ggaacgcgtg gcggcaagtg cggcaaattg gcaggcaatg ggcggcagcc tcaacctgca
     4501 acacttttct tgggataaaa aaaccggcat atcggcaaaa ggcggcgcac acggtctgca
     4561 tatcgccgag ttgcacaatt tcttcaaacc gcccttcgaa cacaatctgg ttttaaacgg
     4621 cgactgggat gtcgcctacg ggcgcaacgc gcgcggctac ctcaatatca gccggcaaag
     4681 cggcgatgcc gtattgcccg gcgggcaggc tttgggtttg aacgcatttt ccctgaaaac
     4741 gcgctttcaa aacgaccgta tcggaatcct gcttgacggc ggcgcgcgtt tcgggcggat
     4801 taacgccgat ttggacatcg gcaacgcctt cggcggcaat atggcaaatg caccgctcgg
     4861 cggcaggatt accgcctccc ttcccgactt gggcgcattg aagccctttc tgcccgccgc
     4921 cgcgcaaaac attaccggca gcctgaatgc cgccgcgcaa atcggcggac gggtcggctc
     4981 tccgtccgtc aatgccgccg tcaacggcag cagcaactac gggaaaatca acggcaacat
     5041 caccgtcggg caaagtaatt ctttcagtac ctcgcccctg ggcgggaaac tcaacctgac
     5101 cgttgccgat gccgaagtat tccgcaactt cctgcccgtc ggacaaaccg tcaaaggcag
     5161 cctgaatgcc gccgtaaccc tcggcggcag catcgccgac ccgcacttgg gcggcagcat
     5221 caacggcgac aagctctatt accgcaacca aacccaaggc atcatcttgg acaacggttc
     5281 gctgcgttcg catatcgcag gcaggaaatg ggtaatcgac agcctgaaat tccgtcacga
     5341 agggacggcg gaactctccg gtacggtcgg tatggaaaac agcggacccg atgtcgatat
     5401 cggcgcggtg ttcgacaaat accgcatcct gtcccgcccc aaccgccgcc tgacggtttc
     5461 cggcaacacc cgcctgcgct attcgccgca aaaaggcata tccgttaccg ggatgattaa
     5521 aacggatcag gggctgttcg gttcgcaaaa atcctcgatg ccgtccgtcg gcgacgatgt
     5581 catcgtatta ggcgaagtca aaaaagaggc ggcggcaccg ctccccgtca atatgaacct
     5641 gactttagac ctcaatgacg gcatccgctt cgccggctac ggcgcggacg ttaccatagg
     5701 cggcaaactg accctgaccg cccaatcggg cggaagcgtg cggggcgtgg gcacggtccg
     5761 cgtcatcaaa gggcgttata aggcatacgg gcaggatttg gacattacca aaggcacggt
     5821 ctcctttgtc ggcccgctca acgaccccaa cctcaacatc cgcgccgaac gccgcctttc
     5881 ccccgtcggt gcgggcgtgg aaatattggg cagcctcaac agcccgcgca ttacgctgac
     5941 ggcaaacgaa ccgatgagtg aaaaagacaa gctctcttgg ctcatcctca accgcgccgg
     6001 cagcggcagc agcggcgaca atgccgccct gtctgcagcc gcaggcgcgc tgcttgccgg
     6061 gcaaatcaac gaccgcatcg ggctggtgga tgatttgggc tttaccagca agcgcagccg
     6121 caacgcgcaa accggcgaac tcaaccccgc cgaacaggtg ctgaccgtcg gcaaacaact
     6181 gaccggcaaa ctctacatcg gctacgaata cagcatctcc agcgcggaac agtccgtcaa
     6241 actgatttac cggctgaccc gcgccataca ggcggttgcc cgtatcggca gccgttcgtc
     6301 gggcggcgag ctgacataca ccatacgttt cgaccgcttc tccggttcgg acaaaaaaga
     6361 ctccgccgga aacggcaaag gaaaataagc ggttttcaga cggcgcgccg ccaaaccgga
     6421 catttgaaaa cctgcttttc caccgtccgc cgccgccgtc cgcctgcaag ggaacagaat
     6481 cgatatagtg aattaacaaa aatcaggata aggcgacgaa gccgcagaca gtacaaatag
     6541 tacggaaccg attcactcgg tgcttgagca ccttagagaa tcgttctctt tgagccaagg
     6601 cgaggcaacg ccgtaccggt ttttgttaat ccgctatatt cc
//
LOCUS       DAXHTY010000096         6612 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXHTY010000096 DAXHTY010000000
VERSION     DAXHTY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.096"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            680..1126
                     /gene="yciA"
                     /locus_tag="ACFNMK_001724"
     CDS             680..1126
                     /gene="yciA"
                     /locus_tag="ACFNMK_001724"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC6364942.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
     gene            complement(1177..1998)
                     /locus_tag="ACFNMK_001725"
     CDS             complement(1177..1998)
                     /locus_tag="ACFNMK_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6364943.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYQRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            2117..2575
                     /locus_tag="ACFNMK_001726"
     CDS             2117..2575
                     /locus_tag="ACFNMK_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6364944.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAASFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2739..3854)
                     /gene="lst"
                     /locus_tag="ACFNMK_001727"
     CDS             complement(2739..3854)
                     /gene="lst"
                     /locus_tag="ACFNMK_001727"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC6364945.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLNKSFDFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            4082..4222
                     /locus_tag="ACFNMK_001728"
     CDS             4082..4222
                     /locus_tag="ACFNMK_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix 2"
                     /protein_id="HFC6364946.1"
                     /translation="MPPHIMHSTSDTAAKRGKTISPFLSRRRVQTRQDRTKREQSRQN
                     MP"
     gene            4289..6514
                     /locus_tag="ACFNMK_001729"
     CDS             4289..6514
                     /locus_tag="ACFNMK_001729"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC6364947.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     TKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1765 a         1924 c         1491 g         1432 t
ORIGIN      
        1 cgtatggatg gattcgtcat tcccgcgcag gcgggaatcc agaccccctg acgcggcggg
       61 aatctatcgg aaatgactga aaccccgaga ttctagattc ccactttcgt gggaatgacg
      121 gttcagttgc gttccgacaa caccgtaatc ttgaaatccg tccgacaaca ccgtaatctt
      181 aaaacccgtc attcccgcgc aggcgggaat ccagacctcc gacgcggcgg gaatctatcg
      241 gaaatgactg aaccccccga gattctagat tcccacttcc gtgggaatga cgtggtgcag
      301 gtttccgtat ggatggattc gtcattcccg cgcaggcggg aatctagacc tgtccgcaca
      361 gaaacttatc agataaaaac agttgctcaa accctgcgtt ctagattccc actttcgtgg
      421 gaatgacggt tcagttgcgt aggactgagt cgtcgaaagg ggcggattcg atggattcga
      481 tgaaaacggt agaaatgtgg gattgatgga aatggcgggc cgaagcccac tctacagccc
      541 cgccctacaa tacgccttgc gaatctgttc cgcgcccgcc cttgtatgtc tcaaatgggt
      601 taaactacgc acttcccgac ttcccgtttc gggcagaggt gcaggttcga tttatatttc
      661 aataaaacaa ggaaacttta tgcagcacga agaaggcaac cgccaacgcc ctcaaggcga
      721 actgctcctg cgtaccgtcg ctatgccgcg cgataccaat cccaaccaag acattttcgg
      781 cggctggatt atgtcgcaga tggatttggg cggcggcata ttggcggcgg aaatcgcgcg
      841 gggacgcatc gttaccgtcg ccgttcagga aatgaacttt atccgcccgg tcaaggtcgg
      901 caacgtcgtc tgctgctacg ggcattgcgt ccgcgtgggc aacacttccc tccagcttaa
      961 agtcgaagtc tgggtgaaaa ctttgatgaa cgattgcgtt accgaagacc gccacctcgt
     1021 aaccgaagcc gtgttcactt atgttgccat cgatgcggaa ggcaatccgc gcccgattcc
     1081 gaaagaaggc aaccccaaat tggcgggctt attgcctact ccgtaaaaat acccgtaaaa
     1141 atgccgtctg aaactgtctt cagacggcat tttgcatcat ttgaaccagg acctgatgcg
     1201 ggaaaacagc gacatactct gtttttcaaa gtcttcccgc acttcgggca cggctttttt
     1261 tatcactttc attcctgcaa aagtctgcgc cacgggcata atttcaatcg tgttcacatt
     1321 catatgcgcc ggccgctggt acagccacaa tgcggtttcc gcaatatctt cggggcggat
     1381 aaattccaca ccctcataca cgcccgccgc cctctcgtca tcgcctttga agcgcacatt
     1441 ggaaaactcc gtattgccgc acaaacccgg ctcgatattg gtaacgcgga tgttcttatc
     1501 cgccaactcc gcgcgcaaat tcaggctgaa ctggcgcaca aacgccttgg tcgccccgta
     1561 aacgttgctg ccggcataag cataattgcc tgcaatcgaa cccaaattca tcacataacc
     1621 gccgccgcgt tccaccattt gcggcaaaat tttgcgcgtc aggaacgtca aacccaaaac
     1681 attggtttga atcatcgttt cccaatcttc aaaatccgcc ttgtccgccg tgtccagacc
     1741 caaagccagc ccggcattgt tgatgaggca gtcgatgtcg gaaaattcat cggggatgcc
     1801 gtttaaggcg ttttccaccg actcgcgtcg cgacacgtcc atttccaaag ggtaaaacaa
     1861 agcacccaat tcatccgcca aggcctgaag cctgtccgca cggcgcgccg caccgataac
     1921 gcggtatccc gccccgacaa acgcacggca catcgcttcg ccgaatcctg ccgaagcacc
     1981 ggtaattaaa acagccattg tttttccttt ctttctcgtt tcccaaacct gtttcaggta
     2041 tcataacacc cttcagacgg catcgcgccg actgaaaatg acaacaacca taccaccacg
     2101 acaggacaca ttcaatatga aaacccaaat cagccttgct gctgccgcca tcacactgct
     2161 cctttccgcc tgcggaggca gcggaatacc ttcccaaccc aaaggcgaaa tttccgaaaa
     2221 ccgcaccgcc gcgttcaaat ccatgatgcc cgaattcaca cgcatgggca aaatggtcaa
     2281 agacgaagaa ccttacgatg tcgaaaaatt caaacaggcg gcagcgtcgt ttgccgaaag
     2341 cagcaagaaa ccgttcacac tttttgagtc cgatccgcaa ggcaacggcc gcgccctgcc
     2401 cgccgtttgg tcggatggtg caaaatttga agccgaaaaa acaaaattcg ccgccgccgt
     2461 cgaaaaactc aacgccgcag cacaaaccgg caaactggat gaaatcaaag ccgcctacgg
     2521 cgaaaccggc gcaagctgca aatcctgcca cgacagtttc cgcgcacccg aataacatcc
     2581 gaaacagaac aatgccgtct gaagccttca gacggcattt tctttgccat ttcaccaagc
     2641 gcaaagcaat caaaaattat cttcaattca aaaaatacgc catatcctaa tttttgcctt
     2701 acttactccg ccattttcta ttttgatttg ctatgcgatt aatctttatc gtcaaatgtc
     2761 aaaatcggga tgccggattg ggtaaacagg gcatacaccg gcttgagcca ataatcttcc
     2821 ggaagggaag ccggtttcaa tgcgtaaacg tgcacattgg gaaaatcctt catcgtcaac
     2881 gccgcaccgc tgaaaaaggt ataaatttca tacctcgtat gcgggttttt cttaatttcg
     2941 cgcaaaatat agtcttcgat gacatagggc gaatttaatg tggttacgcc ggaaagcccg
     3001 taggtttggc gggggtgcgg tgcgacatat tgtatgttga agttttttgc cgccttttcc
     3061 gaaatttcct tcatctcctt gtcgggcgaa cccaaaagta tccgcaccgt gccgcccgtt
     3121 tcatcccccg ccttcagttc ggacgcatcg aacagcggca ggtaagtcat cttgcggcgg
     3181 ccgtcgtcca taatgttttt caaacccttg aatatcgtgt aatgctcgtc ggaagcatta
     3241 cgggttttgg cgatgctcca atctccgatc atcatccggg caaaattacg cttgatcgtc
     3301 ccgtttacag aaaactcatc gcccaaatag ctgctgcttt gaattaaatt gattgtcccg
     3361 tcgtcaaagg ttttgatttc cgcatccggg taagtgctca aaaaggcggc aatgctgact
     3421 ttttccaaac tcgccaaata aatccgcttg accttcggca gcagcatcga cttcaccttc
     3481 agctccgcca tcgtcggaat gaaatcaaac gatttgttca aaccgtaggg caggtagaaa
     3541 aaatacgccc gctccgcctt atcctttatc tgattgaaat aataatcgta tttttcattc
     3601 ctgttttcag acatcagcac cacataaaac cgctcccccg gatgctgcgc cataatcctt
     3661 tctgccacct tcatctgcaa tatggtatag cagaaaatca gattgaccgg ttccccctct
     3721 tcattgaaga gtttgtcctt cagcagggaa accgcgttcc tttccccctg atttacccgg
     3781 tcaaatgtat aaaatatccc gaaacaaaaa acaatcaggc acaacacggt caaacaagcc
     3841 tttttcaagc ccatatccct aaaactccat tccgataaat taaacatacg ccccgagtgt
     3901 cccggggcgg aaaaatgcgg aattttagca gatattccga cagttaccga atattatagt
     3961 ggtttaaatt taaatcagga caaggcgacg aagccgcaga cagtacaagc agtacgacaa
     4021 ggcgaggcaa cgctgtactg gtttaaattt aaaccactat aaaatcaatt tcaccgaaaa
     4081 tatgccgccg cacatcatgc acagcacctc cgacaccgcc gccaaacgcg ggaaaaccat
     4141 ttcccccttc ctttcacgcc gccgcgttca aacacgccaa gatcgaacaa agcgcgaaca
     4201 aagccgccaa aatatgccat aatagccgcg cacattcccc gcgcaatgtg ttccaaaaga
     4261 aaaaaccaac ctacaagaga ataatcctat gactcaaaaa tccaccattg tttataccca
     4321 taccgacgaa gcccccgcgc tggcgaccca atcgctgctg ccgattgtgc aggcgtttgc
     4381 ccgccacgcc gatattgatg tcaaaactgc cgacatttcc ctgtccggcc gtattttggc
     4441 ggcattcccc gaatatctga ccgaagcgca acgcgtaccc gacgcgcttg ccgaattggg
     4501 cgaactggta aaacaacccg atgcaaacgt aatcaaactg ccgaacatca gcgcatccgt
     4561 accgcaactg accgccgcga ttaaagaatt gcagtctaaa ggctttgccg ttcccgacta
     4621 tcctgccgac ccgcaaaccg atgaagaaaa agccgtacgc gaacgctacg accgcatcaa
     4681 aggcagcgcg gtaaaccctg tcctgcgcga aggcaactcc gaccgccgcg cgcctaaagc
     4741 agtgaaaaac tttgccaaaa aaaatccgca cagcatgggc gcatggacaa aagactccaa
     4801 aacccacgtt gccaccatgc aaagcggcga ctttttccat aacgaacaat ccgttaccgt
     4861 acctgaagcg acttccgtat ccatcgtgtt caccgacaaa caaggcaata aaaaagagct
     4921 gcgcgcgcct gttgccctga aagccggcga aatcatcgac gcaaccgtga tgagcaaaaa
     4981 agccctgctc gccttccttg ccgaacaagt gaaagacgcg aaagctaaag gcgtattgtt
     5041 ctcgctgcat atgaaagcca cgatgatgaa agtgtccgac ccgattatct tcggacacgc
     5101 cgtcaaagta ttcttcgcgc ctgtatttga aaaattcggc ggcaaactgg ccgccgcagg
     5161 cgtcaacgtc aacaacggtt tcggcaacct gcttgccaat ctggacaaac tggatgcgga
     5221 cacccgcgcc gccgtcgaag ccgaaatcgc cgccgtttac gccgccaacc ctgatttggc
     5281 gatggttgat tccgataaag gcattaccaa cctgcacgtt cccagcgatg tcatcgttga
     5341 tgcttctatg cctgcgatga ttcgtaactc cggccgtatg tgggataaag acggcaaagc
     5401 gcaagacacc aaagccgtca ttccggacag cagctatgcc ggtgtttacc aagcaaccat
     5461 cgacttctgc cgcgaacacg gcgcattcga tccgacaaca atgggtactg tgcccaatgt
     5521 cggtttgatg gcgcaagcgg cggaagaata cggctcacac aacaaaacct ttgaaatcga
     5581 agccgacggc caagtccaag tcattgatgc ggcaggaaaa gtcctgatgc agcatgacgt
     5641 tgaagcaggc gacatttggc gtatgtgcca aaccaaagac gctccggtta aagactgggt
     5701 gcaacttgcc gtcaaccgcg cccgtctgag caacacgcct gccgtgttct ggctcgacga
     5761 aaaccgtccg cacgacaaga gcctgctcgc caaggttaaa gcctaccttg ccgaactgga
     5821 taccaatggc ctcgacatcc gcgtcctcgc tcctgaagaa gccgccaagt tcagcttggg
     5881 tcgtctgaaa aacggcgaag atactatctc cgtaaccggt aacgtcctgc gcgactacct
     5941 gaccgacttg ttcccgattt tggaactcgg cacaagcgcg aaaatgctgt ctatcgttcc
     6001 attgatgaac ggcggcggta tgtttgaaac cggcgcgggc ggttctgcac cgaaacatgt
     6061 tcaacaattc ctcgaagaaa accacctgcg ttgggactct ttgggcgaat ttctcgcact
     6121 cgccgtatcg tttgaacatc tggcgcaaaa aaccggcaac accaaagccc aagtcctcgc
     6181 cgacactttg gatgcagcca ccgaaaaact gctgttgaac gacaaatcgc ctaaacgtaa
     6241 agccggcgaa ctcgacaacc gcggcagcca tttctacctc accctctact gggcgcaaga
     6301 attggcggcg caagacaaag atgccgaact gaaagccgca tttacgccat tggcagccgc
     6361 tttgaccgcc gacgaagcga aaatcgttgc cgagctttcc gccgtacaag gcaaagcggc
     6421 cgacatcggc ggctactacg ccgccaatcc tgaaaaagcc gcacaagtga tgcgtccgag
     6481 cgtaaccttt aatcaggcac taaacgcctt ataagcacaa aggtaaaaat gccgtctgaa
     6541 cattcgtcgt tcagacggca ttagttatcc ctatctgcct gattatgatt aaacgccgca
     6601 cccattggat at
//
LOCUS       DAXHTY010000097         6476 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXHTY010000097 DAXHTY010000000
VERSION     DAXHTY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.097"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..336
                     /locus_tag="ACFNMK_001730"
     CDS             <1..336
                     /locus_tag="ACFNMK_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC6364948.1"
                     /translation="IDASGLGEKIKDGKKTVLSREEEQKICNTFTHKQAVEDFSVVIG
                     YDEIKAKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKD
                     RLAMLKFNS"
     gene            368..484
                     /locus_tag="ACFNMK_001731"
     CDS             368..484
                     /locus_tag="ACFNMK_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6364949.1"
                     /translation="MPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYRK"
     gene            complement(644..1981)
                     /gene="ribBA"
                     /locus_tag="ACFNMK_001732"
     CDS             complement(644..1981)
                     /gene="ribBA"
                     /locus_tag="ACFNMK_001732"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC6364950.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPSEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHNLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPAADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2195..2851)
                     /locus_tag="ACFNMK_001733"
     CDS             complement(2195..2851)
                     /locus_tag="ACFNMK_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HFC6364951.1"
                     /translation="MILHPSSFNYRDIPKGADFSDNDFVRHFETLVEGRYYIANAWTK
                     IVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGGSISHNIK
                     YKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNIIVIY
                     RKYFSFKEKCRKIFGAKAIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKFFSE
                     "
     gene            complement(2848..3204)
                     /locus_tag="ACFNMK_001734"
     CDS             complement(2848..3204)
                     /locus_tag="ACFNMK_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6364952.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKNA
                     GGFSLIYNNLQTKRLI"
     gene            complement(3360..3953)
                     /gene="ribA"
                     /locus_tag="ACFNMK_001735"
     CDS             complement(3360..3953)
                     /gene="ribA"
                     /locus_tag="ACFNMK_001735"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC6364953.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPQHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3946..4623)
                     /locus_tag="ACFNMK_001736"
     CDS             complement(3946..4623)
                     /locus_tag="ACFNMK_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364954.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNIASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            4777..4861
                     /locus_tag="ACFNMK_001737"
     tRNA            4777..4861
                     /locus_tag="ACFNMK_001737"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4811..4813,aa:Leu,seq:tag)
     gene            5381..6415
                     /gene="purM"
                     /locus_tag="ACFNMK_001738"
     CDS             5381..6415
                     /gene="purM"
                     /locus_tag="ACFNMK_001738"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC6364955.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         1612 a         1582 c         1687 g         1595 t
ORIGIN      
        1 atcgacgcat cgggcttggg cgaaaaaatt aaagacggca aaaaaaccgt actttctcgc
       61 gaagaagaac aaaaaatctg caataccttc acgcacaaac aggcagtgga agatttcagt
      121 gtggtaatcg gctacgatga aatcaaagcg aagaatcaca gcttgtcggc ggggcagtat
      181 ttcgaggtaa agattgatta tgtggatatt tccgccgacg aatttgcgca aaaaatggcg
      241 ggattttcag cggatttgga taaacttttc gccgaatctg ccgaattgga aaaagagatt
      301 aaggatagat tggcgatgtt gaaattcaat tcataaatac cgatgaatga aaccggtcaa
      361 acttaaaatg ccgtctgaaa gcctttcaga cggcattttg tttggtttgg aaaatactta
      421 tgccgttctt tccctgaaag agagaatcca aaaaaccaaa gccacaggaa tttatcggaa
      481 atgacaaaaa cccgacgaac cggattcccg cctgcgcggg aatgacgaat tagaagttac
      541 ccgaaatttg caaaaacaaa atccaaccca acagaccggg ttttcgtttg cacggaaata
      601 atgcaataaa taaagcaaat ataaagtatt tgaatttact atattatttt tccgcttctt
      661 caaagccgac gacttccaaa ccgaagccgg tcagaccggt gaaagatgag ggctgcccga
      721 ggacgcgcag ttttttgacg ttgaggccga cgaggatttg tgcgccgatg ccgtagcttt
      781 tgctgtccca tttgtaggct tggtttgcgc ctttaggcag ggtgcggtcg agcagggatg
      841 cgccgtcttc ggtgcggtgc agaaggatga cgacgccgct ttcggcttgt tggatgcgct
      901 caagggcttt gggcagcgac catgaatggc gcgggttggc ttgaatgaag tccatcacac
      961 tgaagggttc gtggacgcgg acgagggttt cggtgtcggc ggcgggcgtg cctttgacga
     1021 gggcgaggtg ggtttcgccg gagagtttgt cgacgtaaac gtgttgttgg aactcgcccc
     1081 acggggtttg tacaggcgca ttgcccatgt cttcgagcag gctttcggta cggctgcggt
     1141 attcgatgag gtcggcaatg gtgccgattt taaggttgtg ttcttcggca aacttcatca
     1201 gctcgggcat acgcgccatg gtgccgtcgt cgttgatgat ttcacagatg acggaggcag
     1261 gaatcagccc gttcatttgc gccaggtcga cgccggcttc ggtgtgtccg gcgcggacga
     1321 gtacgccgcc tttttgggcg cgaagcggaa agatatgacc gggttggacg atgtcttcgg
     1381 gtttagcggt cggggaaacg gcggtttgaa tagtcagggc gcggtcggcg gcggaaatgc
     1441 cggtggtaat gccgtgtgcg gcttcgatgg agacagtaaa gttggtgccg tattgcgcgc
     1501 cgtttttttg ggtcatcatc ggcagcccga gtttttcgac catttcgccg tccatcggca
     1561 ggcagaccaa gccgcgcgcg tgtttgatca tgaagttgat ggcttcgggc gtgacgaatt
     1621 gcgccgccat cagcaggtcg ccttcgtttt ctcggtcttc ggcatcggtg atgatgacca
     1681 ttttgccggc tttgatgtcg gctaggattt cggggatggg ggagatatgg gacatggtgt
     1741 gttttcctgt gtgttcggac gggcgggcgg tgtgttcggt atcgagccaa aaggcgggca
     1801 tttttgccgc ctgttcgatt ttacgggctt ttttctcgcc gaaggatttg tttttcaaaa
     1861 gggcggagag ttcgcctgtg ttgagggcaa cggcttcagc aaaacgagtt tgcaaaccgc
     1921 cgtagtgttc tgctatccat tgccgcaggt tgcggcggcg cagaccggct gtgtcggtca
     1981 tcgtttcatc ctttgtagaa atcggatgta gcggattgta atcgcgtatt taccaaaaag
     2041 caaatctgtt ttttcgctaa accgaattat ttgctttttg gtaaacagat gccgtctgaa
     2101 gccggattcg tggcttcaga cggcattgcc gcaaccccgc cggcggggcg gctgtttctt
     2161 cccgcccgct tccgattcgg ggtttgccgg aagcttattc cgaaaaaaat ttttgataaa
     2221 cggccttgat tgccgggtac agcataggcg gtacgagcag gcgcaatatc ggcaatccta
     2281 tgatgaatgc ggtcttcccg ataaaaaccg gacggattgc cttcgcgccg aatatctttc
     2341 tgcatttttc tttaaatgaa aaatatttcc ggtagatgac gataatgttt ttggaaaaat
     2401 aaagccttac caatatagac ctcagatagc cgatattgtc gaagacaaat ttttgcaaac
     2461 cgccgtagat gggggaattt ttgttttcga ctaaaaaatc cacaccccgg tcgagatgcg
     2521 tcagcacatc gctgaaattt ttgtatttga tgttgtggct gatagagccg ccgagaacgc
     2581 ggtactggta aaaagggtta tcgtaaaagg caaaagtctt gataaaacgc gccaattgca
     2641 aactgtacgg gaaatcctcg tgaatgtatc cttttgggaa aaacagattg tttttgatga
     2701 tgatttcccg cctgacaatc tttgtccacg cgttggcgat atagtaccgc ccctccacca
     2761 gcgtttcaaa atggcggaca aaatcattat ccgaaaagtc cgcccctttg gggatgtcgc
     2821 ggtaattgaa ggacgaggga tgcaggatca aatcaacctt tttgtctgca agttgttgta
     2881 aatcaaagag aatccccccg cgttttttga acggttggta tcggcccaat agtcgtcgct
     2941 gtccaaaaag attaggtaat cgccttttgc cgcccggata ccggcgttgc gggcatccga
     3001 cagcccgccg ttttcttgat gaatcacttt tatatgcgga tatttgcctg catattcgtc
     3061 gcaaatcttc ccgcagccgt ccggcgaacc gtcatcgacc aaaatcattt cataatcggc
     3121 aaaattttcg gcaagcacgg aatccacgca gcagcggagg tatttttcca cattgtaaat
     3181 agggacgatg atggagaaaa tcataaatat caatccatta tattaagatg tttgcgcgta
     3241 tgcctcaaac ccgcgctcgc aatgcgtttg catccactat aaaacttatt gctttatcaa
     3301 ggtatggaaa cctgtttccc gaaaggcggc gcaggatgcc cgttccctgc aactttgcct
     3361 tattccgaca tcagatgtcc cagcttgtct gctttggttt ggaggtagcg tttgttttca
     3421 aggttttccc cgacgtgctg ggggatgcgt tcgaccacgt taatcccggc atctttcagg
     3481 gtttggattt tttcggggtt gttggtcagc agtttgaccg agcggatgcc cagatattcg
     3541 tagatagatt gcgccaaacg gaaatcgcgg gcatcgacgg gcagcccgag tgccaaattg
     3601 gcttcaaccg tatccatacc ttggtcttgc agatgatagg cgcggatttt gttaatcagc
     3661 ccgatgccgc gtccttcctg acgcagatag acgatgatgc cgcgcccctc tgtctgtacc
     3721 gccctcatgg ccgcttcaag ttgaggtccg cagtcgcatt ttctcgagaa cagcgcatcg
     3781 cccgtcaggc attcggagtg gatgcgcgtc agaaccggat tgccgtctga aaaattaccg
     3841 acggtcagcg cgacgtgttc ctgcccgttt gcctcttcaa agccgtgcat cgtaaatacg
     3901 ccccattcgg tcggcaggcg gcaggatgcc acatggttca acatttcaga catcttcact
     3961 cccatctttt ccggcagaca tacccaacag cagtttcaaa ggttcggaca aagccaaggc
     4021 aagagccacc cattccacct gtgctgccgt acccgcacac tctgcgcatt caaattccac
     4081 atgaaccacg cccaacacac cgccactttc cgtgcagacc ggaatggaaa tttgcgccgc
     4141 cgaagcatga ttgcgttctc ccgaaagctc tcccaaatcc aaccaacgac gcacatccga
     4201 ggcaatattc atccagccgc tttgcgccga acggcaagcc agcgaaacct tgcctgccgc
     4261 ttcatccaaa tcccacaggt tttccaaaac ctcgccctgc cgggacaggc aaaccagccg
     4321 aaaggcggca ttttcagacg gcatcagggc ataaacggct gcggcgcgca cgcctttaca
     4381 gcgtgaaaac accgaatcca aagccatgaa aagccgtttc agaccttcct cattcccgct
     4441 gttttgctcc aaataatcgg caagcctcca atcctcatgc tcaagccaca aaaccgaacg
     4501 ctcaatcgaa gccgtcccca tatccattac cgtctgcgcc gtcagatacg ccgcccgaac
     4561 ctcgtcaagc ggcagcttca aaccctgagt cagcagaaaa tccttaatca aagcagcagg
     4621 catacccaaa ttccgtcaat aaaaacaaaa aaccgcccga ttccgatgtc aggcggacga
     4681 aaaacagatt ttaccgcccc aaaccccaag ccatcaacag ccccgccgaa aaccttgcac
     4741 aaaaaaacaa aatctgtata attgtccaat tcccatgcgg acgtggcgaa attggtagac
     4801 gcaccagatt taggttctgg cgccgagagg tgtgagagtt cgagtctctc cgtccgcacc
     4861 aataatgata aataaatcat atagttaatg cttatcggga caaattagtg ccacaaaaca
     4921 taagtcgtct gcttttcagc gggcggcttt tttatctgcc tatatgtttc aacacgcaga
     4981 acggtacata aagtgccgcc atttgttcta ccccgaatca gaagcgggtg cttccgcccg
     5041 caatcagatt caccgcccct aatgccgggg tttcaactgg aaagaagaaa atattgattt
     5101 tgatgatggc atcaaaaaac cccttccgcc cgtctgccaa aaggaatggt taagaaaagc
     5161 aatgcgtctt ttgatggaac gcgatatata aggaattgag gggaagggca ggcgggtttc
     5221 tgcccgttaa tcctgcggac gatcccgcca aacccgacac gcgcgggcat atggcggatt
     5281 gtttacaata ttgtctaaaa atctgaacct gcaaacgcga aataaggtag aatacgcccc
     5341 gttattttac cgtccaatcc ccatttacca aggaaaaatg atgagtactt cattgagtta
     5401 ccgcgatgca ggtgtcgata tcgacgcagg cgaccaactg gtcgaaaaca tcaaaccgtt
     5461 tgccaaacgc accatgcgtc cggaagtatt gggggatttg ggcggttttg gcgcattggt
     5521 cgaaatcggc aagaaatata aaaatcccgt attggtcagc ggtacggacg gcgtgggtac
     5581 caagctcaaa cttgcctttg attgggataa acacgatacg gtgggcatcg accttgttgc
     5641 aatgagtgtc aacgatattt tggttcaagg agcagaacct ctgtttttct tggattattt
     5701 cgcctgcggc aaattggatg ttccgcgcgc aaccgatgtc atcaaaggca ttgcacaagg
     5761 ttgcgaagaa tccggctgcg ccctgattgg cggggaaact gccgaaatgc cgggtatgta
     5821 tcccgtcgga gaatacgatt tggcgggttt tgccgtcggc gtagtggaaa aagagaatgt
     5881 cattaccggc cgcagcatcg gcgcgggcga tgtggtattg ggtttggctt ccaacggcgc
     5941 acattcaaac ggctattccc ttatccgtaa aatcatcgaa cgcgacaatc ccgatttgga
     6001 tgccgagttt gataatggca aaaccttgcg tgaggctgtt attgcgccga cccgtctgta
     6061 tgtgaaaccg attcttgccg ctttggaaaa atttaccatt aaaggtatgg cacacattac
     6121 cggcggcggc attaccgaaa acgtgccgcg cgtgttgcct gaaaacacgg ttgcacaaat
     6181 cgatgctaaa tcgtgggaat tgcccaagct cttccaatgg cttcaaaagg cgggcaatgt
     6241 ggaaacccaa gaaatgtacc gaacctttaa ctgcggcatc ggcatggtcg ttattgttgc
     6301 tgccgaagat gccgatgcgg ttcagggtct cttgggtgaa caaggtgaaa cagtttaccg
     6361 tttaggtttg atccgtgagc gtcagggaga cgagcatcaa acccaggttg cctgattgct
     6421 tctatagcga aatgccgtct caaacatggg tttggacggc atttttatag aggatg
//
LOCUS       DAXHTY010000098         6455 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXHTY010000098 DAXHTY010000000
VERSION     DAXHTY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.098"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(176..2404)
                     /locus_tag="ACFNMK_001739"
     CDS             complement(176..2404)
                     /locus_tag="ACFNMK_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFC6364956.1"
                     /translation="MRSAVLFIYFFLLNIFLFQPAVIRIQSNYILFMSIVSAPLPALS
                     ALIILAHYHGIAANPADIQHEFCTSAQSDLNETQWLLAAKSLGLKAKVVRQPIKRLAM
                     ATLPALVWCDDGNHFILAKTDGEGEHAQFLIQDLVTNKSAVLSFAEFSNRYSGKLILV
                     ASRASVLGSLAKFDFTWFIPAVIKYRRLFFEVLVVSVVLQLFALITPLFFQVVMDKVL
                     VHRGFSTLDVVSVALLVVSLFEIVLGGLRTYLFAHTTSRIDVELGARLFRHLLSLPLS
                     YFEHRRVGDTVARVRELEQIRNFLTGQALTSVLDLAFSFIFLAVMWYYSSTLTWVVLA
                     SLPAYAFWSAFISPILRTRLNDKFARNADNQSFLVESITAVGTVKAMAVEPQMTQRWD
                     NQLAAYVASGFRVTKLAVVGQQGVQLIQKLVTVATLWIGARLVIEGKLTVGQLIAFNM
                     LSGQVAAPVIRLAQLWQDFQQVGISVARLGDILNAPTENASSHLALPDIRGEITFEHV
                     DFRYKADGRLILQDLNLRIRAGEVLGIVGRSGSGKSTLTKLVQRLYVPEQGRVLVDGN
                     DLALAAPAWLRRQVGVVLQENVLLNRSIRDNIALTDTGMPLERIIEAAKLAGAHEFIM
                     ELPEGYGTVVGEQGAGLSGGQRQRIAIARALITNPRILIFDEATSALDYESERAIMQN
                     MQAICANRTVLIIAHRLSTVKTAHRIIAMDKGRIVEAGTQQELLAKPNGYYRYLYDLQ
                     NG"
     gene            complement(3073..3756)
                     /locus_tag="ACFNMK_001740"
                     /pseudo
     CDS             complement(3073..3756)
                     /locus_tag="ACFNMK_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            4093..4527
                     /locus_tag="ACFNMK_001741"
     CDS             4093..4527
                     /locus_tag="ACFNMK_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364957.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            4803..5522
                     /locus_tag="ACFNMK_001742"
     CDS             4803..5522
                     /locus_tag="ACFNMK_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364958.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
     gene            5740..>6455
                     /locus_tag="ACFNMK_001743"
     CDS             5740..>6455
                     /locus_tag="ACFNMK_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6364959.1"
                     /translation="MIASRLSGNISSAIQEHKDGKVNINRFQNILADLYSLGGLGSTL
                     IEKNGNMQSWGIPLAIAGDIIAATAIATGDTGTISTEEFYNFDNWKGFGYELFEDWSR
                     WVYDWLPDGWNLWKELDRNRSGQYHIYDPLALDLDGDGIETVAAKGFSGSLFDHTNNG
                     IRTATGWVAADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNG
                     DNIINAADAAFQTLRVWQDL"
BASE COUNT         1957 a         1394 c         1353 g         1751 t
ORIGIN      
        1 ttttgcaaag gtctctagat gagtgaaaaa gaagtgcagg ctgcctaaaa agacagaaaa
       61 agtctttccg gcagcctgca ctttggtttc atttcagtca gtaaacccag taaacgacgg
      121 tctgaaaacg cagaacgtta cgaaaaaagc agcctacacg cccatccccc gccttctacc
      181 cgttctgtaa atcatacaga tagcggtaat atccgttcgg cttcgccagc aattcctgct
      241 gtgttcccgc ttccacaatc ctgcctttat ccatggcaat gatccggtgt gccgttttaa
      301 cggtggacag acggtgggcg ataatcagca ccgtccggtt ggcgcaaatg gcctgcatgt
      361 tctgcataat cgctcgttca ctttcataat ccagcgcgct ggtggcttcg tcaaaaatca
      421 gaatgcgcgg attggtgatt aacgcgcggg caatcgcaat acgctgccgc tgtccgcccg
      481 acaagccggc cccttgttcg cccaccacgg tgccgtagcc ttccggcagc tccataataa
      541 actcgtgtgc gcccgccagt ttggctgctt cgataatgcg ttccagcggc atacccgtat
      601 ccgtcagcgc gatattgtcg cgtatgctgc ggttgagcag cacattctcc tgcaagacca
      661 cgccgacctg ccgccgcagc caggcaggag cggccaaagc caaatcgttg ccgtccacca
      721 acacccgtcc ctgctccggt acatacagac gctgcaccaa tttggtgagt gtggatttgc
      781 ccgaccccga acgccccaca atccccagca cttcccccgc ccgaatccgc aggttcaaat
      841 cctgcaaaat cagcctgccg tccgccttat agcggaaatc gacatgttcg aacgtaatct
      901 ccccccggat atcgggcaaa gccaaatgcg aagacgcatt ctcggtcggc gcattcagaa
      961 tatcccccaa acgcgccacc gaaatcccca cctgctggaa atcctgccac aactgcgcca
     1021 aacggataac aggcgccgcc acctgtcccg agagcatatt aaacgcaatc agctgcccca
     1081 ccgtcagctt gccctcaatt accagccgtg cgccaatcca caacgtcgcc accgtcacca
     1141 gcttctgaat cagctgcacc ccctgctggc cgaccaccgc caacttcgtt acccgaaatc
     1201 ccgaagccac ataagccgcc aactgattgt cccaacgctg cgtcatctgc ggctccaccg
     1261 ccatcgcctt taccgtaccc accgcagtga tgctttctac taaaaacgac tggttgtctg
     1321 cattgcgcgc gaacttatcg ttcagacgcg tccgcagtat cggactgata aatgccgacc
     1381 aaaacgcata ggcaggcaac gaagccaata ccacccaagt cagagtggag ctgtaatacc
     1441 acatcaccgc cagaaagata aacgaaaacg ccaaatccaa caccgaagtc agcgcctgac
     1501 cggtcaggaa attgcgaatc tgctccaatt cccgcacccg agccaccgta tcacccactc
     1561 gtctgtgctc gaaataggat aaaggcaggg aaagcagatg ccggaacaaa cgcgcgccca
     1621 attccacatc aatacgtgaa gtcgtatgtg caaacagata cgtccgcaaa ccgcccaaca
     1681 caatctcaaa cagcgacacc accaacaaag ccaccgacac cacatccaaa gtagagaatc
     1741 cccgatgtac cagcaccttg tccatcacca cttggaaaaa cagaggcgta atcagcgcaa
     1801 acagctgcaa caccaccgac accaccaata cttcaaaaaa caaccggcgg tatttgatta
     1861 ccgccggaat aaaccaggta aagtcaaact ttgccaaact gcccaatacc gaagcgcggg
     1921 aagcaaccaa tatcagtttg cccgaatatc tgttagaaaa ttcggcaaaa gacaataccg
     1981 cagacttatt cgtaaccaaa tcctgtatca aaaattgggc atgctcaccc tcaccgtctg
     2041 ttttggccaa aataaaatgg ttgccgtcat cacaccatac caatgcgggt aaagtcgcca
     2101 tagccaaacg tttaataggc tggcggacta cctttgcctt caatcccaaa gatttggcgg
     2161 ccaaaagcca ttgcgtttca tttaaatcgc tctgtgcgga agtacaaaat tcatgctgta
     2221 tatcggcagg attggcggca atgccgtggt aatgggcgag gatgatgagg gcggaaaggg
     2281 cggggagcgg tgcggatacg atagacataa ataaaatata gttagattgg atgcggataa
     2341 cggctggctg gaaaaggaat atattaagta gaaagaaata tataaataaa acagcagaac
     2401 gcattgtaag gatatatatg ggaattgtaa agagaaagta tggaaaagtt ctcgtttcag
     2461 gaaggtaaaa cggcttagga atcgagttag atgaggatgc ctcgcacctc tcgtgcctcc
     2521 tgcataccgt taaggcacag ggttaaggtg caggctgctc cgaactctgt tgcggtcggg
     2581 taatgttatt ttttgtgttt caggcagcct gaaatatctg tatatttttg ttttaaatag
     2641 attttaaaga ttgataactg ttcttgacga tttttcaaga aaggagtaaa tttcaagaaa
     2701 ggagtaaagt gacttattat caatgacaag caacgcgcga agtgacaagg aaaactatct
     2761 acttaaattc taaggaggct tcgaatatca taaaccaatc agaaacatag agataaaaat
     2821 tatgtacaaa tataatcctc ttatacaatt tattgcacag ttgattatgt cttatggagc
     2881 aagcgtaggg tgggcacttg ctgccccacg cgtttcatat ttcaaggcag cctgaaaccg
     2941 tgtgggcata aatgcctacc ctacatccca aaaaacaagc gcagcctgcg tgtgtagggt
     3001 gcgaactttc ggcaggtaga cacgcagttt tatattttca agctgaggga tgcttaagaa
     3061 aagtacaaaa cattaaaaaa taaggggctg tactagatta gccctaaatc cacaccaatc
     3121 ccgcaagatt tttagctgtc gggacggtgt gccgaagtta aatcgaaatt cgcattcttt
     3181 caagaacagc gggaaagatt tgcgatcaat tccgttctat ttgcgcaaga cgcgttttgc
     3241 ctgattccaa aagttctcaa tgccgtttat gtggttctga cggtcagcaa attccttgga
     3301 atggttgatg cggtaatgga taaaaccgct tacgtccaac ttgtcgtaac tgctcaggct
     3361 gtcggtataa acaatgctgt ccggcatgat tttctgtttg ataacaggca ttaaagtatc
     3421 ggacttggca ttatctacga caacggtata gacccgtccg ttacgtttca gaatgccgaa
     3481 gacaaccact tttcctgccg caccgcgacc acgtttgcct ttacgccgtc cgccgaaata
     3541 gctttcgtcc aactcgacag agccctcgaa aacctcattg gcagccaagg ccagataatg
     3601 gctgatgacc atacggattt tgcggtagaa caggactgcc gaattgggat ggatacccaa
     3661 aatatcggca gcagaacggg cggtaacttc gagtacaaaa aaacggagca gttctttctg
     3721 tacttttttc tttaatttgc agtgtgttat cttcatattt cgggggtaac atatctgcta
     3781 atctagtaca gcctcaaaca aaaaagagaa attttaattt cgctaaatcg cataaagatt
     3841 aatcaagagt atcattaaat gatatgagtg agcatctata atgccaagaa gagttgttaa
     3901 gacataacga ttattgaaat agattgtaaa atagatactt agatagtctg aaaaacggat
     3961 ttgtgaaact ttttattacg cgccatcatt tgaaaatgaa acttaaaaaa cacttatcat
     4021 aataaatatt ttctttacgt tgtttgctaa taaactcagt gcaatatcag cgcaatattt
     4081 tatggaaatt ttatggataa caaaaaagaa tttattaata atttaacaaa taggtatatg
     4141 tggatctatc cattggtctt aaatattcta tttctacctt tttaccagtc ctaccaatct
     4201 ttttttattg cgcttggttg tttgtttgca ctggttagaa aaatgcaaag cttagatttt
     4261 aaattacaaa atcatattgt attgttaaat ataaaaagtg cttgggcaga taaaaaagta
     4321 tttttgatta ggatagtagt gtcatggttg gcagtaatgg aaatatggat gtgttttatt
     4381 tcggaatcat caacgtgggt atgcggtgct ttttgtttaa atagtgaaat attggaaaaa
     4441 atttttcgtg gctttggtta ttctggtagt ttatattttt tatttatatt gatgattgat
     4501 ctcaataagt taagagagag tatttgaatt tcattttttg tttgacttaa actcaaggag
     4561 agtaacaatg attggtagtg gtgatactaa acaatgcaaa aaattttctg cgtgtgatgg
     4621 aaaataccac gtctacgatc ccctcgccct agacttggac ggcgacggca tagaaacagt
     4681 caccgccaaa ggcttttcag gcagcctgaa gactgagaga gtgaatacga tgagtataca
     4741 ctctatgcca ctaaattgat attcactaaa tcataccagc tatattttat ttaatgagac
     4801 atatgaaaaa taaaaattat ttactagtat ttatagtttt acatatagcc ttgatagtaa
     4861 ttaatatagt gtttggttat tttgtttttc tatttgattt ttttgcgttt ttgttttttg
     4921 caaacgtctt tcttgctgta aatttattat ttttagaaaa aaacataaaa aacaaattat
     4981 tgtttttgtt gccgatttct attattatat ggatggtaat tcatattagt atgataaata
     5041 taaaatttta taaatttgag catcaaataa aggaacaaaa tatatcctcg attactgggg
     5101 tgataaaacc acatgatagt tataattatg tttatgactc aaatggatat gctaaattaa
     5161 aagataatca tagatatggt agggtaatta gagaaacacc ttatattgat gtagttgcat
     5221 ctgatgttaa aaataaatcc ataagattaa gcttggtttg tggtattcat tcatatgctc
     5281 catgtgccaa ttttataaaa tttgcaaaaa aacctgttaa aatttatttt tataatcaac
     5341 ctcaaggaga ttttatagat aatgtaatat ttgaaattaa tgatggaaac aaaagtttgt
     5401 acttgttaga taagtataaa acattttttc ttattgaaaa cagtgtttgt atcgtattaa
     5461 ttattttata tttaaaattt aatttgcttt tatataggac ttacttcaat gagttggaat
     5521 agttttggta attttatgag cgcacgctca tccgcgttag cagaatttgg aaatatggtt
     5581 gctaatttag tttctgcaaa aaatgagaaa gatatctcga aacgtaatga atattacaaa
     5641 caagctggtt atagtgcatt attagcattt ggtaatttgg ctagtaatat tgcaccaggt
     5701 agtacgtcat cgcatattgt aaacggaaca aatgcctctg tgattgcaag ccgtctctct
     5761 ggaaatatat cttcagctat tcaggagcat aaagatggta aagttaatat caaccgtttt
     5821 caaaatattt tagcggattt atattcattg ggagggttag gaagtacatt aatagagaag
     5881 aatggaaata tgcagagttg ggggattcca ttagcaattg ctggagatat aattgcagca
     5941 acggctattg ccacaggaga tactggtacg atatctacag aggaatttta taattttgac
     6001 aactggaaag gttttgggta tgagctattt gaagactggt ctcgttgggt atacgactgg
     6061 ctgcccgacg gctggaatct gtggaaagaa ttggacagaa accgttcagg ccaataccac
     6121 atctacgacc ccctcgccct agatttggac ggagacggca tagaaactgt cgctgccaaa
     6181 ggcttttcag gcagcttatt tgatcacacc aacaacggta tccgcaccgc caccggttgg
     6241 gttgctgccg atgacggtct gcttgtgcgc gatttgaacg gcaacggcat catcgacaac
     6301 ggcgcggaac tcttcggcga caataccaaa ctggcagacg gttcttttgc caaacacggc
     6361 tacgcggctt tggccgaatt ggattcaaac ggcgacaaca tcatcaacgc ggcagacgcc
     6421 gcattccaaa ccctgcgtgt atggcaggat ctcaa
//
LOCUS       DAXHTY010000099         6241 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXHTY010000099 DAXHTY010000000
VERSION     DAXHTY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.099"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(63..623)
                     /gene="sodC"
                     /locus_tag="ACFNMK_001744"
     CDS             complement(63..623)
                     /gene="sodC"
                     /locus_tag="ACFNMK_001744"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFC6364960.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="ACFNMK_001745"
     CDS             complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="ACFNMK_001745"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC6364961.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQILAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENALWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLLITPFEAQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2243..3188
                     /locus_tag="ACFNMK_001746"
                     /pseudo
     CDS             2243..3188
                     /locus_tag="ACFNMK_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3200..3430)
                     /locus_tag="ACFNMK_001747"
     CDS             complement(3200..3430)
                     /locus_tag="ACFNMK_001747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364962.1"
                     /translation="MPSEKPFPFQTAFCRLRAQSAYTAALIRHTAPKMPIRLPHNKNN
                     GQFIRQQPDSPFYAAEKMPSETHSDGIRLKNN"
     gene            complement(3445..4083)
                     /locus_tag="ACFNMK_001748"
     CDS             complement(3445..4083)
                     /locus_tag="ACFNMK_001748"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC6364963.1"
                     /translation="MSKLTPREILSAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAS
                     HNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4264..6099)
                     /gene="edd"
                     /locus_tag="ACFNMK_001749"
     CDS             complement(4264..6099)
                     /gene="edd"
                     /locus_tag="ACFNMK_001749"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC6364964.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHFARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKAGNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
BASE COUNT         1399 a         1492 c         1740 g         1610 t
ORIGIN      
        1 ctaaatatct agcaaaaaag tgcggtcaaa atttaccgca cttttcgttt cgaacaatcg
       61 aattatttaa tcacgccaca tgccatacgt gggccgccac cgccaagtgg agctggatga
      121 tcggagtgat tatcaccacc cgtgtggatc ataatagagt gaccgcgaac atcatctaaa
      181 tgtttaagac gtggtgctaa aacaggattt gttgctgtgc catcatgcaa tacagttaat
      241 gcaggtaaat cacctaagtg tgcatcatct tgccatgggt aaccatgttg ttttgcacct
      301 ttaggatccc agtgaccgcc tgcgcctaaa ccagctgtca atttaccttc tttttctttt
      361 ggctcacagc ttgggttttc atggatgtgg aaaccatgta agccttcgct taatccttgt
      421 aaatcagggg taaacacaag accatagtta gattcagtaa tagtcactgt acccacatct
      481 ttgttaccgt ttactggatc aagttgttgc actttcactt caatagaagc acctttaggt
      541 atcgtattat gctcgtgtgc ttgcgcaaca ccaactgaac atactgcact aaccgctagt
      601 gctaataagg ttttcatatt cattgcaatc tcctttattg cagtaaaaac tcgcgactat
      661 tttagtcaat tttgttttaa aagccgataa atttttacga aaattttaca ttaattgtta
      721 tttgcagaaa attagaaccc gttatcacgt cttggataaa tgccaaaacc attgtgattt
      781 agagaattaa aaagaaaaat ttcggaatcc tcatcctttt ctgaaacctg attgcggtat
      841 ccatggcttt gtctttgtca tttaagtttg tgttcatgtt tttgaccttc tttgcttttt
      901 aaatcctttt taaattgccg acttcttccg ttccaaatgt cccatttgaa cattatcccg
      961 ccgcctaaaa tacgacccaa attcatcggt tgttcttgct gcgatacctg caaacaacag
     1021 ttcaatgggt ttattttgtt tattctaacc tattaccgtt taaataaatt tccaaaggct
     1081 tgggcaaacc gtaatctttc aaatgcgccg gctttatcca aatgccgtct gaagggcttt
     1141 cagacggcat ttgtgcttca aagggcgtaa tcaatagcag ccggtgcgtc aggcggtggg
     1201 tcagggcggt ttgttcgtcc atatctgcca tggtcaggga gaatttggcg gcaaagtcgg
     1261 aaagcccgtt caaactttca aaacacggca cgcaatacag cccgccccaa atgcctttgg
     1321 cggggcgttt ttccagcaaa atcgcgccgt cccggttgcg gacaatcagc cagtaaagcg
     1381 gcagggtcgg tacttcggcg gcggtttttt tgcgcggcag ctcggcggtg cggttttgct
     1441 ttttcgcttc gcagatgtcc gccatcgggc attggtggca caagggtttc gtccgtttgc
     1501 acacggtcgc gcccaaatcc atcaaacctt gtgtataggc aggcatatcg gcgttttcag
     1561 acggcagcag gctttcggca agtgtccaga gcgcgttttc aaattttttg tcttgcggat
     1621 tgccgtcttg ggcaaagaca cggcagagta cgcgtttgac gttgccgtcc aaaatggtct
     1681 cgcggcggtt gaaggcaaag gcgcaaatgg cggcggcggt gcttctgcct acgccgcaga
     1741 gggtttccaa gtctttacgc tccgacggaa acgtgccgcc gaattgcctg acgacttgtt
     1801 gcgcggcttt gtgcagattg cgcgcgcggc tgtaatagcc caagcctgcc cacaaggata
     1861 aaacctcgtc ttgcggcgcg gcggcaagta tttgaacagt cgggaatttt tctaagaaac
     1921 gcggatagta gtccaacacg gtggcgacct gcgtttgctg gagcatgatt tcggaaagcc
     1981 agacgcaata agggtttttg acctgccaag ggaggtggtg gcgaccgtgt tgtttttgcc
     2041 agcggatgag ccgttcggag aaggggatgg gtgtgttcat taatatcaat cggtggtttt
     2101 atttatattt aaaacagtat gttattgtat aacattgtga aaataattct tatttactta
     2161 ttttttgtag gggcatataa ctcatataaa gaaactttat tgcggtattg aaattattta
     2221 tcaacaagca aggagtatca gcatgaaagc aatggtttat cacggcgcaa acgacatccg
     2281 ttttgaagaa aaaccccgtc cgcagattat cgatccgacc gatgcggtgg tgaaaatcgt
     2341 caaaaccacg atttgcggta ccgatttggg tatttggaaa ggcaaaaaaa cccgaagtcg
     2401 ccgacggtcg tattctcggt catgaaggca tcggtattgt agaagaagtc ggcgaggctg
     2461 taaaaaacat caaagtcggc gataaagtca ttatttcatg cgtcagcaaa tgctgtactt
     2521 gcgacaactg caaaatccaa ctttattcgc attgccgcaa cggcggctgg attttgggct
     2581 acatgattga cggcacgcag gcagaatacg tccgcacgcc ttatgccgac aacagcctcg
     2641 tgccgctgcc cgacaacgtc aacgaagaag tcgccctgtt gttgagcgat gccttgccga
     2701 ccgcccaact gtacagcccc gccgccatca ttgtttgcga tatggacgaa aaccgtttga
     2761 aactggcgaa agagttgggc gcaacccata ccattaaccc tgcttccggc gatgtaagca
     2821 agcaagttgc cgctatcgtc ggcgaagacg gcgtggactg cgcgatcgaa gccgtcggta
     2881 tccctgcaac ttggaatatg tgccaagaca ttgtgaaacc cggcggccat atcgccgttg
     2941 tcggcgtaca cggtcaatcc gttgatttca aactggaaaa actgtagatt aaaaagcttg
     3001 ccatcactac cggcctggta aacgccaata ccaccgaaat gctgatgaag gcaatttcca
     3061 gcagctccgt cgattacacc aaaatgttga cccaccgttt caaattcagc gaattggaaa
     3121 aagcctacga cgtgttcaaa cacgccgccg aaaatcaagc gatgaaagtg gttttggaag
     3181 cggattaatc ggtcttgaat cagttgtttt ttaagcggat gccgtctgaa tgcgtttcag
     3241 acggcatttt ttcagctgcg taaaacgggc tgtccggctg ctgtcgaatg aactgaccgt
     3301 tatttttatt atgcggaagg cggatgggca ttttaggggc ggtatgtctg atcagtgctg
     3361 ccgtgtatgc cgactgtgcc ctcaatcggc aaaatgccgt ctgaaacggg aaaggttttt
     3421 cagacggcat ttttacgatg cgaatcaggc tttgggtttc aacgccgccg cttctttggc
     3481 gaggcgggtg atggtgtccc agtctttgtt tttcacagct tctttcgggg tcagccaaga
     3541 gccgccgacg cacaagacgt tgggcagtgc caggtaatcg ggtgcggtgg cgaggctgat
     3601 gccgccggtc gggcagaagc gcacgtcggc ataaggaccg taaagggctt tgagcatggc
     3661 tttgccgccg acgacttcgg cggggaaaag tttgagggtg tcgatgccgt gttccaaagc
     3721 cagttggact tcgcccggag tggcaacgcc gggaatcagg gggatgccgc tgttgtggct
     3781 ggctttggcg agggattcgt gcaaacccgg gctgatggcg aaaaccgcgc ctgcgtcttc
     3841 gacggctttg agctgttcgg ggttggtaac cgtacctgcg ccgacgatgg cgttgggtac
     3901 ttctttggca atcaggcgga tggcgtcgag gccgacgggg gtgcgcaggg taatttcgag
     3961 ggtggggatg ccgccttcga caagggcgtg ggacaaatcg acagcggtgc tcaagtcgtc
     4021 aatcgccatc acaggcacaa ctgcgcctgc gctcaggatt tcgcgggggg tcagtttgga
     4081 catttcggtt ctccgggtgg aatggggtat tttattaaga tgggacaggt cgtaggtcgg
     4141 attcaagaaa ccgacatctt ggacggctgt gtcggattcg agaatccgac ctacgtctat
     4201 cttaaaacac ttgaataatt tttcagacgg ccttcggtct gaaaaggccg tctgaaacgc
     4261 gcatcaggca aattcgccgc cgaaactcat ggcaccggtt tccgcgctgc tggtcatgct
     4321 gcggaagttg gcgaagagtt cgcggccgca gccttgttgg ttcgcgccca agtcgatgct
     4381 ttcgacttcg cgggcattcc attcggtttc gttaatcagg acgttgagtt cgccgctaac
     4441 ggagtcgaag cggatcaggt cgccggtacg gattttggcg atgttgccgc ccatcagggc
     4501 ttcgggtgtc atgtggatgg aggccggaac tttgccagat gcgccggaca tacgaccgtc
     4561 ggtcagcagt gccactttga agccgcggtc ttgcaggatg cccaaaggcg gggtcagttt
     4621 gtgcaattcg ggcataccgt tggcacgcgg gccttggtag cggacgacgc agacgaaatc
     4681 gcgttccaac tcgccgcgtt caaatgccgc caacacttcg cgttggtcgt tgaacacgat
     4741 ggcaggcgct tcaataatgc ggcagccttc gcgcacggcg gacactttaa tcacgccgcg
     4801 tccgatgttg cctttcatca ggcgcagacc gccgtcgggg gagaacgggt tgccggcttt
     4861 gcgcaggatg tcgtcgttgc cgctggtttc gggggcttcg cgccattcga gtttgccgtc
     4921 gatgaggaaa ggctctttgg tgtagtggcg cataccgtgt ccgacgacgg tatcgacatc
     4981 gtcgtgcaac aggcctgcat tcagcaattc gcggataacg aaaggcagcc cgcccgctgc
     5041 ggtaaagtga ttcacgtcgg ccttgccgtt cggataaacg cggatgagca gcgggatgat
     5101 ggaggaaatt tcgtcgaagt cgtcccagtt caaaatcacg ccggccgcac gcgccatagc
     5161 gacgaggtgc atggtgtggt tggtcgaacc gccggttgcc atcaggccaa tcaaggcgtt
     5221 gataaaggat ttttcggtca acatttcgcc caaaggttta atcgtgccgt ttttgatgcc
     5281 gcgcgcgaag tgtccggcgg cgtagcgggt cagcgcttcg cgcaggtcgg tgtaagggtg
     5341 gacgaaggcg gcggcaggca ggtgcacgcc catcatttcc atcatcattt ggttggagtt
     5401 tgccgtgccg tagaaagtgc aagtacccgg gctgtggtaa gagcccattt cactttttaa
     5461 aagttcgtcg cgtccgacct tgccttcggc gaaaagctgg cgggtgcggg ctttttcttt
     5521 gttgccgata ccgctggaca tcgggcctgc ggggacgaag atacccggaa tatgaccgaa
     5581 cgaaagcgcg cctatcatca aacccggcac gattttgtcg catacgccca taaacaggct
     5641 gccgtcaaac atttgatgcg acagaccgat agcggtactc atggcgatca cgtcgcggga
     5701 aaacagcgac aattccatac cggcgtagcc ttgcgtgatg ccgtcgcaca tggcgggtgt
     5761 gccgccggcg acttgggcgg tcgcgccgtt tttctgcgct tcgtctttga tttggtcagg
     5821 gaagtcttta aacggctggt gtgcggaaac catgtcgttg taggcggtga tgatgcctaa
     5881 gttggggaca gtttcctgaa gcatttcgat tttgatgctt ttaggcatgg cggcatagcc
     5941 gtgcgccaaa ttgctgcagc cgagttggtt gcgctccaag cgtcccattt gtttggcgct
     6001 gcggattttc gccagatatt tttcacgcgt cggacggctg cgttcgataa tgcgcccggt
     6061 gatttcggcg agtttggagt ggataggagt agtgttcacg gtcggtctcc tgtcggaacg
     6121 gggtggtatt ggggatttgt ttgtgcgttt ctgatttttt gtattgggtg tggggcattt
     6181 ggcgtttcag acgacctttc aaaccttaca aggggtcgtc tgaaaacctc actatagtgg
     6241 a
//
LOCUS       DAXHTY010000100         6064 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXHTY010000100 DAXHTY010000000
VERSION     DAXHTY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.100"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            25..408
                     /locus_tag="ACFNMK_001750"
     CDS             25..408
                     /locus_tag="ACFNMK_001750"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC6364965.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            523..996
                     /locus_tag="ACFNMK_001751"
     CDS             523..996
                     /locus_tag="ACFNMK_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC6364966.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1122..2225
                     /gene="mnmA"
                     /locus_tag="ACFNMK_001752"
     CDS             1122..2225
                     /gene="mnmA"
                     /locus_tag="ACFNMK_001752"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC6364967.1"
                     /translation="MNTTANTTNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2297..3967
                     /locus_tag="ACFNMK_001753"
     CDS             2297..3967
                     /locus_tag="ACFNMK_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC6364968.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETISFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITDTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4079..5713
                     /locus_tag="ACFNMK_001754"
     CDS             4079..5713
                     /locus_tag="ACFNMK_001754"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC6364969.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            5941..>6064
                     /locus_tag="ACFNMK_001755"
     CDS             5941..>6064
                     /locus_tag="ACFNMK_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6364970.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQ"
BASE COUNT         1593 a         1655 c         1522 g         1294 t
ORIGIN      
        1 tgagaacatt ttgaaaggat accgatggaa ccttcttctt acgcggcaga aaaaaaaggc
       61 aaaggcggca tcaggcgcgt cattaacgca ttcggctatt cgatagacgg catcgccgcc
      121 gcctaccgtt acgaagcggc attccgtcag gttttgtggc tgaacgtact gctggtgtgc
      181 gcggcatttt tttgggtttc cgaaaccgcc gtccgcctgc cgttgattat cgcgtctttt
      241 gtgtcggtca ttgtcgaact gttcaacacc gccgtcgaag ctgccgtcga tcatacttcg
      301 accgaaaaac acgagctggc caaacgcgcc aaagacgcag gttctgccgc acaattgttc
      361 gcgatgctga tgttggcggc ggtttggctg tctgccctgt tcgggtaaaa cgcttgcagc
      421 aggatttgta atcctttagg attagtatgt ggtatttatt attcacttaa tccactataa
      481 gattaagcac attccccgtc tgatattata aggagcagaa agatgaaaaa attattggcg
      541 gccgtgatga tggcaggttt ggcaggcgcg gtttccgccg ccggagtcca cgttgaggac
      601 ggctgggcgc gcaccaccgt cgaaggtatg aaaataggcg gcgcgttcat gaaaatccac
      661 aacgacgaag ccaaacaaga ctttttgctc ggcggaagca gccccgttgc cgaccgcgtc
      721 gaagtgcata cccacatcaa cgacaacggc gtgatgcgga tgcgcgaagt cgaaggcggc
      781 gtgcctttgg aggcgaaatc cgttaccgaa ctcaagcctg gcagctatca cgtgatgttt
      841 atgggtttga aaaaacaatt aaaagagggc gacaagattc ccgttaccct gaaatttaaa
      901 aacgccaaag cgcaaaccgt ccaactggaa gtcaaaatcg cgccgatgcc ggcaatgaac
      961 cacggtcatc accacggcga agcgcatcag cactaatctg ctgaaaatgt ttgaaatgcc
     1021 gtctgaaaaa gcccgggctt tcagacggca tttttatgcc cgcctttaaa atgtgttaaa
     1081 atccgccttt aaaaaccgcc gtttccaacc atattctacg catgaatacg acagcaaaca
     1141 ccaccaatat catcgtcggg ctctccggcg gtgtcgattc ttccgtaacc gccgccctgc
     1201 tcaagcagca gggttatcaa gtgcgcggtg tgttcatgca gaactgggaa gacgacgaca
     1261 acgacgaata ttgcagcatc aaacaggatt cgttcgatgc catcgccgtt gccgatatta
     1321 tcggcatcga tatcgacatc gttaatttcg ccgcgcaata taaagacaaa gtctttgctt
     1381 attttcttca ggaatacagt gcggggcgca cgccgaatcc cgatgtgttg tgcaacgccg
     1441 aaatcaaatt caaatgcttt ttggattacg ccgtagggca gggcgcggat accatcgcca
     1501 ccggacacta tgcgcgcaaa gaagtccgca acggcgtgca ttacctgctc aaaggtttgg
     1561 atcgaaacaa agaccaaagc tatttcctct accgcctcaa gcctttccaa ctcgagcgcg
     1621 cgatttttcc gttgggcggt ttggaaaaac ccgaagtccg ccgccttgcc gccgagttca
     1681 aactgccaac cgctgccaaa aaagacagca cgggcatctg cttcatcggc gaacgtccgt
     1741 ttcgcgagtt cctgcagaaa tacctgccga ccgacaacgg taaaatggtt acgcccgaag
     1801 ggaaaaccgt cggcgaacac gtcgggctga tgttctacac gctcggacag cgcaaaggtt
     1861 tgggcattgg cggtgcgggc gaaccgtggt ttgttgcggc taaagatttg acgaaaaacg
     1921 aactgattgt cgtacaagga cacgaccatc cgctgctcta tacccgcagc cttgtgatga
     1981 acgatttgag tttcacgctg cccgaacgcc cgaaagcagg gcgttatacc tgtaaaacgc
     2041 gttaccgtat ggcggacgcg ccttgtgagt tgcgctattt ggatgaagaa accgccgagc
     2101 tggtgtttga cgaaccgcaa tgggcggtta cgccgggcca gtctgccgtg ctgtacgacg
     2161 gcgacatctg tttgggcggc ggcatcattc aaacgaccga caagcctgtc atcatcacac
     2221 gataaaactt atgccgtctg aaacagtttt cagacggcat tgttccgctc aattccactt
     2281 taaaagaccg atacctatgg aaaaaatctg gttagacagt tacgagaagg gcgtcagtgc
     2341 cgaaatcgac atcacgcaat acggttccgt cagcgatgta ttttgccaaa gtgtcaagaa
     2401 atttgccggc caacctgctt ttcaaaacat gggcaagacg ctgacttatg ccgaaaccgg
     2461 caaactggca acagattttg cttcttatct gcaaaacgtc ctgaagctgc cgcgcggcga
     2521 acgtgttgcc attatgctgc ccaacgtatt gcaatacccg attgcccttt tcggcatttt
     2581 gcaggcaggt ttggtagcgg tgaacaccaa tccgctctat accccgcgcg agctggagca
     2641 tcagttgaaa gacagcggcg cgaccgccat catcgttttg gaaaatttcg ccaacacgct
     2701 ggagctggtg ctgccgcgca cgcagatcaa acacgtcatc gtagcctccg tcggcgaaat
     2761 gttcgggctg cttaaaggtt cgctgatcaa tttcgtcatc cggaaaatca agaaaatggt
     2821 gcccgaatac cgtattcggg aaaccatttc ctttcagacg gcattgaaag agggggcgaa
     2881 gcacgttttc caacctgtcg cattaaaccg cgaagatacc gcgctgttgc aatacacggg
     2941 cggcacgaca ggtgttgcca aaggggcggt gctgagccac ggcaacatct gcgccaatat
     3001 gcttcaggca aaagaatgga ttaaaaacca gttgcgtgag ggcaaagaaa ccgttatcgc
     3061 cgccctgccg ctgtatcata ttttcgcctt aaccgtgaat ctgatgattt ttgccaatgc
     3121 cggctcgaaa atcatcctga ttaccaaccc gcgcgatatg aaaggcttta tcggagaact
     3181 gaaaaaacag cgggttaatg tatttatcgg cgtgaacaca ctgtttaacg cgatggttaa
     3241 ccggcccgat tttgccgaag tcgatttttc aggattgcgg ctgactttgg gcggcggtat
     3301 ggcgacccaa aaagccgttg ccgaaaaatg gaaaaaaatc accgacacgc ccatcgtcga
     3361 agcctacggt ctgaccgaag ccagccccgg cgtgtgctgc aaccccttaa acatcgaatc
     3421 atacagcggc agcatcggtt tgcccgtctc gtccaccgaa gtcgaactgc gcgacgcaaa
     3481 cggcaaagaa gtccccgtcg ggcagccggg cgaattgtgg gtaaaaggcc ctcaagtgat
     3541 gcaaggctac tggaaccgcc ccgaagaaac cgccaaagcc atagacgcgc gcggcttctt
     3601 ggaaaccggc gatattgccg tcatggacga aaaaggctgg ttgaagctgg tcgatcgtaa
     3661 gaaagacctc gtcgttgttt ccggattcaa tgtttatccg aacgaaatcg aggaagtcat
     3721 cgcgcatcac ggcaaagtga tggaagtcgc ctgtatcggc gttcccgacg aaaaaaccgg
     3781 agaagcccta aaagtatttg tcgtcaaaaa agacccgtca ttgaccaaag aagaacttat
     3841 cgccttttgc cgcaccgaat taaccgcata taaagtaccg aaaaatatcg aattccgcga
     3901 cgagttgccc aagtccaacg tcggcaaaat cctgcgccgc gagttgcggc aaagtaccgg
     3961 gaaataaaga aaagatgccg tctgaaaaca gccgtccacc gttcagacgg catccgtccg
     4021 tttgccagaa ccgcgcgctt cacgttaaaa tcacgcattc caacatgggt attccatcat
     4081 gaccaaattc attttcgtca ccggcggcgt tgtctcctca ctgggtaaag gtatcgccgc
     4141 cgcttctatt gccgccatcc tcgaatcgcg cggcttgaac gttaccatgc tcaagctcga
     4201 tccttatatc aacgtcgatc ccggcacgat gagcccgttc caacacggcg aagtgttcgt
     4261 caccgacgac ggcgcggaaa ccgacctcga cttgggacac tacgaacgct ttatcgattc
     4321 cacgatgacc cgccgcaaca gcttcagcac ggggcaggtg tacgaaaacg tcatcgccaa
     4381 agaacgacgg ggcgactatt tgggcggcac ggttcaagtc atcccgcaca ttaccgacga
     4441 aatcaaacgc cgcatccacg aaggcgcggc aggttacgat gtggcgattg tcgaaatcgg
     4501 cggcacggtc ggcgacatcg aatcgctgcc gtttttggaa gccatccgcc agatgcgaag
     4561 ccagttggga cgcaacaaca ccctgttcgc ccacttgagc tacgtcccct acatcgccgc
     4621 tgcaggcgaa atcaaaacca agccgaccca gcacaccgtt aaagaaatgt tgagcatcgg
     4681 tttgcaaccc gacatcctga tttgccgtat ggacaggaca atgcctgcgg acgaacgccg
     4741 caaaatcgcc ttgttctgca acgtggaaga acgcgcgatt gtcggcagct acgatgtgga
     4801 cagcatctac gaatgccccg aaatgctgca cgaccaaggc atcgacaaca tcattaccga
     4861 gcagttgcag cttaatgtgc agcaggcgga tttgaccgcg tggaaaaaaa tcgtccacgc
     4921 catccaaaac ccgaaacaca ccgtcaaaat cgccatggtc ggcaaatacg tcgatttgac
     4981 cgagtcctac aaatcgctga tcgaagcctt gaaacacgca ggcattcaca ccgaaaccga
     5041 cgtacaaatt acctttgttg acagcgaaag catcgagaaa aacaacggcg acgtttctat
     5101 gctcaaagat atggacgcca tcctcgttcc cggcggtttt ggttcgcgcg gcgtggaagg
     5161 caaaatcgcc gctgtgcgct atgcccgcga aaacaacgtg ccatacttgg gcatctgcct
     5221 cggtatgcag attgcgctga tcgaatacgc ccgcgacgtg gcaggtttga aaggtgcgaa
     5281 ttccaccgag ttcgacctca aatgcgccgc ccccgtcgtt gccctgattg acgaatggca
     5341 aactgccgac ggcagcgtcg aaacccgcga cgaatccgcc gatttgggcg gcaccatgcg
     5401 tttgggcgcg caagaagtcg aattgaaagc aggcagcctc gccgccaaaa tctacggcag
     5461 cgaacacatc cgcgaacgcc accgccaccg ctacgaagtc aacaacaact acgttcctac
     5521 gctggaacag gcaggtttgg tcatcggcgg cgtatccgcc ggacgcgaac gcttggtcga
     5581 aaccatcgaa ctgccgaacc atccttggtt cttcgcctgc cagttccacc ccgagtttac
     5641 ctccaatccg cgcaaagggc atcctttgtt caccgcgttt gtcaaagctg cgttgaacaa
     5701 taaaaaagcc tgataaagcg ttacttgatg atcaaaatgc cgtctgaaag cctgaatgaa
     5761 gactttcaga cggcattttc gcaaatcggg gattataaaa gccatcaatt taatttttat
     5821 ggttttatct aatatttacc aactgaatat tgttaagcgg ggcgtgaaac ttaagttgag
     5881 acctttgcaa aattccccaa aatcccctaa attcccacca agacatttag gggatttctc
     5941 atgagcacct tctttcaaca aaccgcccaa gccatgattg ccaaacacat cgaccgcttc
     6001 ccactattga agttggacca ggtgattgat tggcaaccaa tcgaacaata cctgaaccgt
     6061 caaa
//
LOCUS       DAXHTY010000101         5975 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXHTY010000101 DAXHTY010000000
VERSION     DAXHTY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.101"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(240..401)
                     /locus_tag="ACFNMK_001756"
     CDS             complement(240..401)
                     /locus_tag="ACFNMK_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364971.1"
                     /translation="MPSENHETGFRRPYILFFLVFRYMKSSLKMGRKAGFAAGCSLIF
                     NYIAIDMKS"
     gene            complement(427..1494)
                     /gene="murG"
                     /locus_tag="ACFNMK_001757"
     CDS             complement(427..1494)
                     /gene="murG"
                     /locus_tag="ACFNMK_001757"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC6364972.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDDARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGGLNREKCLKWAENA
                     RTLALPHSADDVAEAAIECTA"
     gene            complement(1498..2823)
                     /locus_tag="ACFNMK_001758"
     CDS             complement(1498..2823)
                     /locus_tag="ACFNMK_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC6364973.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(2949..4286)
                     /gene="murD"
                     /locus_tag="ACFNMK_001759"
     CDS             complement(2949..4286)
                     /gene="murD"
                     /locus_tag="ACFNMK_001759"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC6364974.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCDLN
                     MTDCATLEEAVQKAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            complement(4374..4754)
                     /locus_tag="ACFNMK_001760"
     CDS             complement(4374..4754)
                     /locus_tag="ACFNMK_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6364975.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAEQGNAAAQ
                     YNLGAMYYKGQGVRRDDAEAVRWYRQAAEQGLAQAQNNLGVMYAERRGVRQDRALAQE
                     WLGKACQNGNQDGCDNDQRLKAGY"
     gene            complement(4869..5951)
                     /gene="mraY"
                     /locus_tag="ACFNMK_001761"
     CDS             complement(4869..5951)
                     /gene="mraY"
                     /locus_tag="ACFNMK_001761"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC6364976.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         1418 a         1739 c         1544 g         1274 t
ORIGIN      
        1 ctgagcaaag tgagtgcaca aagccatctg aaggcgatgt gtttgaacct tttgaaagcg
       61 gccaacaggc taagtgcgcc cgctgccgcc taaaaagcag cccggatgcc tgattatcgg
      121 gtgtccgtgg aggattaagg gggtatttgg gtagaattag gaggtatttg gggcgaaaat
      181 agacgaaaac ctgtgtttgg gtttcggctg tcgggaggga aaggaatttt gcaaaggtct
      241 caggatttca tatcgatggc aatatagtta aaaataaggc tgcacccagc ggcaaaccct
      301 gctttcctgc ccattttcaa actcgatttc atataccgaa acaccagaaa aaacaaaata
      361 taaggccgtc tgaaaccggt ttcgtggttt tcagacggca tctgtttcgt ttatcgcatc
      421 atgtcatcat gccgtacact cgatagcggc ttccgccacg tcgtccgcac tgtgcggcag
      481 tgccaacgta cgggcgtttt ctgcccattt gaggcatttt tcgcggttta agccgccgag
      541 aatctcggcg agtttttccg ccgtcaactg ggtttgcggc aacagcaatc ccgcctccgc
      601 ctgcaccata aaacgcgcgt tggcggtttg gtgatcgtca accgcgtggg ggtacggaac
      661 gagcaacgca cccaatcccg ccgccgtcaa ctcggcaatc gtcagcgcgc cggcacggca
      721 aatcaccaaa tcggcatcgc ggtaggcgga caccatgtcg gtaataaatt ccacgcattc
      781 ggctttcacg cccagcgcgt cgtaatccgc ctgcaagctg cccagcttgc cccgtcccga
      841 ttggtggtac atctgcggac gcgcatcgtc gggcagcaaa gccaatgcct gcggtacggt
      901 tttgttcaaa acgtccgcgc ccaaactgcc gccgaccacc aaaattttca gacggccttc
      961 acgcccttgg aagcgttcgg caggcacggg caggttgcta atatcggcgc ggacggggtt
     1021 gccgaccaag ccgccttcgt ggctgaacgc tttcggaaaa gcgtacaaca cccgcttcgc
     1081 ccagcgcgac aggtggcggt tggacaaacc tgccacggcg ttttgctcgt gaatcacaat
     1141 cggcacgcct aatagcttcg ccgccaaacc gccggggaag gtaacgaagc cgccgaagcc
     1201 gatgacgcac tcgacacggt gtttgcggat aatccgctgc gcttcgcgga cggtttgata
     1261 caaagtaacc ggcagcatca gtttgcgttt gatgccgttg ccgcgcacgc ctttaatcgc
     1321 cagcgtttcc aagcgtatgc cgtattgcgg cacgatacgc tcttccatcg aatccttgct
     1381 gcccagccaa atcacatgat ggccgcgcgc gcgcaatgaa tccgccaccg ccagcgcggg
     1441 gaaaatatgt ccgcccgttc cgcccgccat cagcataaag gttttaccgc ccatgattta
     1501 ctccacccga taaccgcgca ttttccggcg gttttcataa tctatacgca acagcagcat
     1561 catgctgatc agcatgaaaa agactgacga accgccatag gacatcaacg gcagcgtcag
     1621 acctttggtc ggcaaagcac cgatgttcac accgatattg aagaaacttt ggataccgat
     1681 ccaaatgccg atacccgaag cgatataggc gttgaaagtc aaacccaaat cgcgcgactg
     1741 cttgccgatg gaaaacgccc gcaccaccag ccagccgtaa cagaatatca gcacgcacat
     1801 accgaagaaa ccgaattctt cggcgatgat ggcaaaaata aaatcggtat gcgcttccgg
     1861 cagaaagccg cgtttgctca aactcgcacc caaacccata ccgaaccact ctccgcgccc
     1921 gattgccatc agagagtggg taagctggta gccggcaccc tgcgggtctt tccacgggtc
     1981 caaaaatgcc actacccgct gcacacggta gggagcggcg gtaatcatca gcaccatccc
     2041 gcccaagacg ctgcctacca ggacgaaaaa atatttccac ggcaatcctg ccaaaaacag
     2101 cattccaacg gcaatgacgg taatgacgac aaacgaaccg aaatccggct gtaccattat
     2161 cagcaccaaa ccgaacgcca ccagcataat cggcagaatg atcgcccgga aacggccgta
     2221 catttctaat gtttcacgac gtgcctgcgg attggtggcg gacatgatca gattggccgt
     2281 cccccgccaa atcgactgcc aacccaaact ttccatgctg cgcaacactt cttcacggcg
     2341 cgtgaacagg cttgccaaat aaaggatgac cgccagcttg aacagctcgg tcggctggaa
     2401 attcaacggg cccaaaggta tccaacgggt cgcgccattg atttcgcgcc cggcaatcaa
     2461 tacgactacc agcaacaggc cggatagggc aaaaatccac ggcacaagcc gccgccatgt
     2521 cctcatcctg caaagaaacc ataaaaaacc gctcgctatc aagccggcaa cgacgaaccc
     2581 cgcctgtctg gtcaaataga aaaactgatc gccgccttct tttgatgcca aatacacaga
     2641 agccgaataa atcatcagca ggctgaacgc cgtcatcagc accaccatcc acaaaagcgg
     2701 cgcgtcgaat ttcctgccgt cgcgcacaat cggcctgtcg agcagcagag tgtggacacc
     2761 gtcgcccact tttaccaata cttccgaaat cttcaaaaaa accacctgcc agtctgtttg
     2821 cacctgccgc aaagggcaaa aatttcagac ggcagacaat gccgtccgaa catacgatac
     2881 atcccaaatc ggtattctaa atctttactt gccgcccaac aatgacggcg tttgcgtttc
     2941 agacggtatc acaaagcctt aaacgccccg ataaacactt ccgaacggtg cgcatagcct
     3001 ttaaacatat cgaagcttgc gcaggcgggg ctgagcaaca cgatgtcgcc cgcttcggct
     3061 tgggcatatg ccttctgaac cgcttcttcc aaagtggcgc agtcggtcat attcagatcg
     3121 cagccgtcca aatcgcggcg gatttgcggc gcatcgacac cgatcaggaa cacgcctttt
     3181 gccttgccgg caagcgcgtc gcgcaggggc gtgaagtcct gccctttgcc catgccgcct
     3241 aaaatcacga agagcggatt ttgcaaaccg gcaatcgcgg cggctgtcgc gccgacgttc
     3301 gtgcctttgc tgtcatcgat aaacaccacg ccgtttttct cgccgatttt ttccacgcgg
     3361 tgcggcaggc cttgaaaggt tttgacgtgt tcgagcaatg cttcgcgcgg caaaccgacg
     3421 gcttcgcaca aggcaacggc agccatcacg ttagtggcgt tgtgcaagcc ttgcaacgga
     3481 atatcttgcg tggcaatcaa atcttcattg ccttgtttca ggcggcctgt ctcgcgttcc
     3541 aaccaaaaat cggcttcgtg ttccaacgaa aaccatttta cctcgcgccc tgcccgtttc
     3601 atggcacggc agaacgcatc gtccgcattc aaaacctgca caccgtcgcc acggaaaatc
     3661 ttggctttgg tatgcgcgta gtcgagcaag tcgtcgtagc ggtcgagatg gtcttcggaa
     3721 atgttcagta ccgtcgccgc agtcgggcgc aggctttcgg tgttttccag ttggaagctg
     3781 gaaagctcca acacccacac gtccgccttt ttgccttcgc gctgccattc cgcctccaaa
     3841 accggcgtgc cgatattgcc cgcgataacg gtatccagcc cgcacttgat gcagagatag
     3901 ccgaccaggc tcgttaccgt ggttttgccg ttgctgccgg taatcgcaat taccttgtcg
     3961 cctcggcggt tcacaatgtc cgccagcaat tcgatgtcgc ccaacacgcg tccgccgttt
     4021 tgcttgaacg cctcgatatc cggctgccgc tcgctgatgc cggggctgag tgccagaata
     4081 tcgaaaccgt tgtccagcgc atctttcaga cggcccgtat aaaacaccag cccgtcaaac
     4141 atcttaccga tttgcgacac ccgctccggt ttcagctccg catcataagc ggcaacctcc
     4201 gcgccgtttt tgcgtaggta ggcaatcatg gaaatacccg tgccgccgag tccggcgacg
     4261 aggatttttt tgttttggaa agtcattttg gttttgtcct aaaacaaatc atattgggca
     4321 ggagggaatg tccgctcctg ctcaagccgc tttcagacgg catcgcgagc cgattaataa
     4381 cccgccttca ggcgttggtc attgtcgcag ccgtcttggt ttccgttttg acaagccttg
     4441 ccaagccatt cttgtgcaag ggcgcggtct tggcgcacgc cgcgtctttc ggcatacatc
     4501 acgcccaaat tgttttgggc ttgggctaac ccctgttccg ccgcctgccg ataccatctg
     4561 accgcttcag catcatcccg gcgcacgcct tgtcctttgt aatacattgc gcccaaattg
     4621 tattgggctg ctgcatttcc ctgctctgcc gcctgcaagt tttcccgaaa atccgatacg
     4681 tcatccgccc acaccgcttg gttcaagccc aaggcaatca gggcggcggc aagccatttg
     4741 actgtctgtt tcatggtttt acttctgttt tagtataagc cgccgttaac gatagggctg
     4801 ggcggattgt cgccgcaggt ttattgcgcg ttcaaatgcc gtctgaaaga tgttcagacg
     4861 gcataggttc agcggatttt gagggtactc aagccgatta acaccaagac gatggtaata
     4921 atccaaaagc ggacgacgac ttgggtttct ttccagcctt tttgttcgta gtggtggtgg
     4981 atgggcgcca tcaggaagat gcgttttttg gttttcttat accaaccgac ctgaagcata
     5041 acggatacgg cttctacgac aaataatccg cccataatga cgaggacaaa ctcttggcgg
     5101 acgatgacgg cgacggtacc gagcgcggca cccaatgcca atgcaccgac atcgcccata
     5161 aagacttgcg cgggataggc gttaaaccac aagaaaccga ggcacgcgcc gcacatggcg
     5221 gtacagaaaa tcaccacttc gtttgcgccg gcaacgtaag gtaattgcag gtattgggca
     5281 aattgtgagt ggccgctggc ataggcgaag atggcgaggc cggcggcaac gaggacgacg
     5341 gggaaggtcg caaggccgtc caagccgtcg gtgaggttga cggcattgga tgtgccgacg
     5401 atggtcaggt aagacaacac caaaaagccg accacgccca gcggcagggc gatttgtttg
     5461 aagaacggga caatcaaaat attgttggcg gaattggcgg caaggtaaaa caatgccaaa
     5521 ccggcgataa tggcaacgct tgactgccac accattttga atttggcgga cacgccgttg
     5581 gggtctttat agacgacttt gcgccagtcg tcgtaaaaac cgagtgcgcc cgtggcgagc
     5641 aatacgccca agagaatcca gatatacggg tttgcccagt tgccccacaa cagggtggac
     5701 acggtaatgg cggtcagaat cagcgaaccg cccatcgtcg gcgtgccgtt tttgacgagg
     5761 tgggtttgcg gaccgtcggt acgcactgcc tgcccgcatt tgagcgcggt cagcctgcgt
     5821 atcgtccacg ggccgaacat cagggaaaac gccaaggcgg tcaacgccgc catgacggcg
     5881 cggaatgtgg tgtattgaaa aatattcaga ccggttaacc agttgctgaa atgtgcgagc
     5941 cataaaaaca tggggcttcc tttttttgtt ttgtc
//
LOCUS       DAXHTY010000102         5970 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXHTY010000102 DAXHTY010000000
VERSION     DAXHTY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.102"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..419)
                     /locus_tag="ACFNMK_001762"
     CDS             complement(<1..419)
                     /locus_tag="ACFNMK_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HFC6364977.1"
                     /translation="MLCPEKMSVMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVGAPA"
     gene            complement(455..568)
                     /gene="rrf"
                     /locus_tag="ACFNMK_001763"
     rRNA            complement(455..568)
                     /gene="rrf"
                     /locus_tag="ACFNMK_001763"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(665..3556)
                     /locus_tag="ACFNMK_001764"
     rRNA            complement(665..3556)
                     /locus_tag="ACFNMK_001764"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3963..4038)
                     /locus_tag="ACFNMK_001765"
     tRNA            complement(3963..4038)
                     /locus_tag="ACFNMK_001765"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4003..4005),aa:Ala,seq:tgc)
     gene            complement(4044..4120)
                     /locus_tag="ACFNMK_001766"
     tRNA            complement(4044..4120)
                     /locus_tag="ACFNMK_001766"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4084..4086),aa:Ile,seq:gat)
     gene            complement(4221..5761)
                     /locus_tag="ACFNMK_001767"
     rRNA            complement(4221..5761)
                     /locus_tag="ACFNMK_001767"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1290 a         1749 c         1291 g         1640 t
ORIGIN      
        1 gcgggcgcgc cgaccgaagc ctggtccttc agcttcgcca gcaccgcagg gccgatgccc
       61 tttaccttgg tcaaatcgtc tacagacttg aacgcaccgt tttgcgcacg gtattccgca
      121 atggccttcg ccttcgcagg gcctatgccc ggcagcgcct ccaactcctg ctgcgaagcc
      181 gcattgatgt ttaccgccgc aagggagaag gcgcaggaga acagcataca gaacagcacg
      241 aacattttct tcatggtttt tcctttaagg gttgcaaaca ataaaccgca tcttgcgacg
      301 ataaaacggg tcattctaaa atgaatatcc caaagtttca agccgttcct ccgcaaaccc
      361 gaccggacgc cgtacggatg ccgtcccgcc atcaccgaca ttttttccgg gcaaagcaaa
      421 aacccccgaa taatcggggg ttttctgaat gggtgtttgg cagtgaccta ctttcgcatg
      481 gaagaaccac actatcatcg gcgctgagtc gtttcacggt cctgttcggg atgggaaggc
      541 gtgggaccaa ctcgctatgg ccgccaaact taaactgtta caaatcggta aagccttaat
      601 caatatattc ggtaatgact gaatcagtca gtaagctttt atctcttgaa gttcttcaaa
      661 tgatagagtc aagcctcacg agcaattagt atgggttagc ttcacgcgtt accgcgcttc
      721 cacaccccac ctatcaacgt cctggtctcg aacgactctt tagtgcggtt aaaccgcaag
      781 ggaagtctca tcttcaggcg agtttcgcgc ttagatgctt tcagcgctta tctcttccga
      841 acttagctac ccggctatgc aactggcgtt acaaccggta caccagaggt tcgtccactc
      901 cggtcctctc gtactaggag cagcccccgt caaacttcca acgcccactg cagataggga
      961 ccaaactgtc tcacgacgtt ttaaacccag ctcacgtacc actttaaatg gcgaacagcc
     1021 atacccttgg gaccgactac agccccagga tgtgatgagc cgacatcgag gtgccaaact
     1081 ccgccgtcga tatgaactct tgggcggaat cagcctgtta tccccggagt accttttatc
     1141 cgttgagcga tggcccttcc atacagaacc accggatcac tatgtcctgc tttcgcacct
     1201 gctcgacttg tcggtctcgc agttaagcta ccttttgcca ttgcactatc agtccgattt
     1261 ccgaccggac ctaggtaacc ttcgaactcc tccgttacgc tttgggagga gaccgcccca
     1321 gtcaaactgc ctaccatgca cggtccccga cccggatgac gggtctgggt tagaacctca
     1381 aagacaccag ggtggtattt caaggacggc tccgcagaga ctggcgtctc tgcttcaaag
     1441 cctcccacct atcctacaca agtgacttca aagtccaatg caaagctaca gtaaaggttc
     1501 acggggtctt tccgtctagc agcgggtaga ttgcatcttc acaaccactt caacttcgct
     1561 gagtctcagg aggagacagt gtggccatcg ttacgccatt cgtgcgggtc ggaacttacc
     1621 cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt actggggctt
     1681 cgatccgatg ctctcacatc ttcaattaac cttccagcac cgggcaggcg tcacacccta
     1741 tacgtccact ttcgtgttag cagagtgctg tgtttttaat aaacagtcgc agccacctat
     1801 tctctgcgac cctccggggc ttacggagca agtccttaac cttagagggc ataccttctc
     1861 ccgaagttac ggtatcaatt tgccgagttc cttctcctga gttctctcaa gcgccttaga
     1921 attctcatcc tgcccacctg tgtcggtttg cggtacggtt cgattcaaac tgaagcttag
     1981 tggcttttcc tggaagcgtg gtatcggttg cttcgtgtcc gtagacactc gtcgtcactt
     2041 ctcggtgtta agaagacccg gatttgccta agtcttccac ctaccggctt aaacaagcta
     2101 ttccaacagc ttgccaacct aaccttctcc gtccccacat cgcatttgaa tcaagtacag
     2161 gaatattaac ctgtttccca tcgactacgc atttctgcct cgccttaggg gccgactcac
     2221 cctacgccga tgaacgttgc gcaggaaacc ttgggctttc ggcgagcggg cttttcaccc
     2281 gctttatcgc tactcatgtc aacattcgca cttctgatac ctccagcaca ctttacaatg
     2341 caccttcatc ggcctacaga acgctcccct accatgccgg taaaccggca tccgcagctt
     2401 cggttataga tttgagcccc gttacatctt ccgcgcagga cgactcgacc agtgagctat
     2461 tacgctttct ttaaatgatg gctgcttcta agccaacatc ctggctgtct gggccttccc
     2521 acttcgttta ccacttaatc tatcattggg gaccttagct ggcggtctgg gttgtttccc
     2581 tcttgacaac ggacgttagc acccgctgtc tgtctcccga ggaaccactt gatggtattc
     2641 tgagtttgcc atgggttggt aagttgcaat aaccccctag ccataacagt gctttacccc
     2701 catcagtgtc ttgctcgagg cactacctaa atagttttcg gggagaacca gctatctccg
     2761 agtttgttta gcctttcacc cctatccaca gctcatcccc gcattttgca acatgcgtgg
     2821 gttcggtcct ccagtacctg ttacggcacc ttcaacctgg ccatggatag atcactcggt
     2881 ttcgggtcta cacccagcaa ctattcgccc tattaagact cggtttccct acgcctcccc
     2941 tattcggtta agctcgctac tgaatgtaag tcgttgaccc attatacaaa aggtacgcag
     3001 tcacacctct agggcgctcc cactgtttgt atgcatcagg tttcaggttc tgtttcactc
     3061 ccctcccggg gttcttttcg cctttccctc acggtactgg ttcactatcg gtcgatgatg
     3121 agtatttagc cttggaggat ggtcccccca tattcagaca ggatttcacg tgtcccgccc
     3181 tacttttcgt acgcttagta ccgctgttga gatttcgaat acgggactgt cacccactat
     3241 ggtcaagctt cccagcttgt tcttctatct cgacagttat tacgtacagg ctcctccgcg
     3301 ttcgctcgcc actacttgcg gaatctcggt tgatttcttt tcctccgggt acttagatgg
     3361 ttcagttctc cgggttcgct tctctaagtc tatgtattca acttaggata ctgcacagaa
     3421 tgcagtgggt ttccccattc ggacatcgcg ggatcattgc tttattgcca gctcccccgc
     3481 gcttttcgca ggcttacacg tccttcgtcg cctatcatcg ccaaggcatc cacctgatgc
     3541 acttattcac ttgactctat catttcaaga acttctttga ctttgcctaa cattccgttg
     3601 actagaacat cagacttgaa tttcctactt tgataaagct tactgctttg ttgtgtctta
     3661 atcctgcctt ttgtgtttca ggattaagtc gatacaatca tcacccaaat actgtgtttg
     3721 ttttcttttc tcttgtgagc gattttttat cctttgcaaa gaataaaaaa tcaaaacaaa
     3781 cgctttgtct ttgtttgttg atttcggctt tccaatttgt taaagatcga tgcgttcgat
     3841 attgctatct actgtgcaaa tcaaaacgag ctgattatta tatcagcatt ttgttcttgg
     3901 tcaagtgtga cgtcgccctg aatggattct gttccattct tccgttttga tttgtacagt
     3961 attggtggag gcaaacggga tcgaaccgat gaccccctgc ttgcaaagca ggtgctctac
     4021 caactgagct atgcccccgt tcttggtggg tctgggagga cttgaacctc cgaccccacg
     4081 cttatcaagc gtgtgctcta accagctgag ctacaaaccc ggattctctt cttaagcgaa
     4141 tcttgccttc actcaagctt cttccgcatc ttttcagttt accgataagt gtgaatgcct
     4201 aaagcctctt ctttctctag aaaggaggtg atccagccgc aggttcccct acggctacct
     4261 tgttacgact tcaccccagt catgaagcat accgtggtaa gcggactcct tgcggttacc
     4321 ctacctactt ctggtatccc ccactcccat ggtgtgacgg gcggtgtgta caagacccgg
     4381 gaacgtattc accgcagtat gctgacctgc gattactagc gattccgact tcatgcactc
     4441 gagttgcaga gtgcaatccg gactacgatc ggttttgtga gattggctcc gcctcgcggc
     4501 ttggctaccc tctgtaccga ccattgtatg acgtgtgaag ccctggtcat aagggccatg
     4561 aggacttgac gtcatcccca ccttcctccg gcttgtcacc ggcagtctca ttagagtgcc
     4621 caactgaatg atggcaacta atgacaaggg ttgcgctcgt tgcgggactt aacccaacat
     4681 ctcacgacac gagctgacga cagccatgca gcacctgtgt tacggctccc gaaggcactc
     4741 ctccgtctcc ggaggattcc gtacatgtca agaccaggta aggttcttcg cgttgcatcg
     4801 aattaatcca catcatccac cgcttgtgcg ggtccccgtc aattcctttg agttttaatc
     4861 ttgcgaccgt actccccagg cggtcaattt cacgcgttag ctacgctact aagcaatcaa
     4921 gttgcccaac agctaattga catcgtttag ggcgtggact accagggtat ctaatcctgt
     4981 ttgctaccca cgctttcggg catgaacgtc agtgttgtcc caggaggctg ccttcgccat
     5041 cggtattcct ccacatctct acgcatttca ctgctacacg tggaattcta cctccctctg
     5101 acacactcga gtcacccagt tcagaacgca gttcccgggt tgagcccggg gatttcacat
     5161 cctgcttaag taaccgtctg cgcccgcttt acgcccagta attccgatta acgctcgcac
     5221 cctacgtatt accgcggctg ctggcacgta gttagccggt gcttattctt caggtaccgt
     5281 catcagccgc tggtattagc aacagccttt tcttccctga caaaagtcct ttacaacccg
     5341 aaggccttct tcagacacgc ggcatggctg gatcaggctt gcgcccattg tccaaaattc
     5401 cccactgctg cctcccgtag gagtctgggc cgtgtctcag tcccagtgtg gcggatcatc
     5461 ctctcagacc cgctactgat cgtcgccttg gtaggccttt accccaccaa ccagctaatc
     5521 agatatcggc cgctcgaata gcgcaaggcc caaaggtccc ctgctttctc tctcaagacg
     5581 tatgcggtat tagctgatct ttcgatcagt tatcccccac tactcggtac gttccgatat
     5641 gttactcacc cgttcgccac tcgccacccg agaagcaagc ttctctgtgc tgccgtccga
     5701 cttgcatgtg taaagcatgc cgccagcgtt caatctgagc caggatcaaa ctcttatgtt
     5761 caatctctaa ctttttaact tctggtctgc ttcaaagaaa ccaacaggac aatgttcaaa
     5821 acattatctt gtctgtcttt caaacagtgt gaggctcaag gcactcacac ttatcggtaa
     5881 tctgttttgt taaagagcgt tgcgaattat aaagtatttc ttccgcctgt caagatatct
     5941 ctcgatatcc ccaacattct gtgctatact
//
LOCUS       DAXHTY010000103         5722 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXHTY010000103 DAXHTY010000000
VERSION     DAXHTY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.103"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(445..3186)
                     /gene="tbpA"
                     /locus_tag="ACFNMK_001768"
     CDS             complement(445..3186)
                     /gene="tbpA"
                     /locus_tag="ACFNMK_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HFC6364978.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDSSEYAYFIVEDECEGKNYETCKSKP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDANSKQAGSLPGNGKYVGNHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYFQNAKRAYSLNTPPQNGKNSPNGSQDKPYWVSIGGGNVVTGQICLFGNNT
                     YTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDSSVSTGTHRTLSW
                     NAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIVF
                     KGDFGNLEASWFNNAYRDLIVRGYEAQIKNGKEEAKGDPAYLNAQSARITGINILGKI
                     DWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQP
                     EGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIKK
                     HFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF"
     gene            complement(3273..5429)
                     /locus_tag="ACFNMK_001769"
     CDS             complement(3273..5429)
                     /locus_tag="ACFNMK_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6364979.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRLKRRNWYPQAKEDEVKLDESDWEATGLPDEPKELPK
                     RQKSVIEKVETDSDNNIYSSPYLKPSNHQNGNTGNGINQPKNQAKDYENFKYVYSGWF
                     YKHAKREFNLKVEPKSAKNGDDGYIFYHGKEPSRQLPASGKITYKGVWHFATDTKKGQ
                     KFREIIQPSKSQGDRYSGFSGDDGEEYSNKNKSTLTDGQEGYGFTSNLEVDFHNKKLT
                     GKLIRNNANTDNNQATTTQYYSLEAQVTGNRFNGKATATDKPQNSETKEHPFVSDSSS
                     LSGGFFGPQGEELGFRFLSDDQKVAVVGSAKTKDKPANGNTAAASGGTDAAASNGAAG
                     TSSENGKLTTVLDAVELTLNDKKIKNLDNFSNAAQLVVDGIMIPLLPQNSTGENNQPD
                     QGKNGGTAFIYKTTYTPKNDDKDTKAQTGAAGSSGAQTASGTESVTGGQAGTKTYEVE
                     VCCSNLNYLKYGLLTRKTAGNTVGSGNSSQAAAQTAQGAQSMFLQGERTDEKEIPKEQ
                     QDIVYRGSWYGHIANGTSWSGNASDKEGGNRAEFTVNFGDKKITGTLTADNRQAATFT
                     IEGDIEGNGFSGTAKTDNLGFDLDQSDNTRTPKAYITDAKVQGGFYGPKAEELGGWFA
                     YPGDKQTEKATVASGDGKSASSATVVFGAKRQQPVQ"
BASE COUNT         1193 a         1439 c         1443 g         1647 t
ORIGIN      
        1 aatttaatgt tgcggcacta gccaaaaaac cgaaaccgaa cggactagat tcccgcctgc
       61 gcgggaatga cggattttag gttggggtca tttattggga aaagcagaaa ccgctctgcc
      121 gtcattccca cgaaagtggg aatccagttt tttgagtttc agtcattccc gataaattgc
      181 cttagcattg aatgtctaga ttcccgcctg cgcgggaatg acgggatttg aggtttctgt
      241 ttttgatttt ctgttttcgt aggaatgacg gtttagagtt acccgaaacc tcaaaaaaac
      301 ctaaaccgaa cggactggat tcccgcctgc tcgggaatga cggatttcag gattacggtg
      361 tatcggggat gatgggaaac ggtgggaatt atgtaaaaaa tgccgtctga agcctttcag
      421 acggcatttg cggcgtttgg acgtttagaa cttcatttcc aagctaaatg tgtagtttcg
      481 gccgggggcg gcatatcggt tgtaaacgcc gacatttttg tgttggttga ctgcgccgcc
      541 ggcagtttgc cgcacatttt cccaagtaac atagcggtag ttgagaaggt tgtacacgcc
      601 cgcacggagg gtgaagtgtt ttttaatcgt gtaataaccg gacacatcca caatatacca
      661 agggcgggta cggcgcgcgg tggcttttgt attgcggctg ttgccgttga gcaaagcccg
      721 gctgcccaac aactctgtga tttccttggc tttggaataa gtcagcatac cgttcacacc
      781 ccatttgcct tccggttggt catagcccaa gccgacgaca tagcgcgagg gttggatggc
      841 atcaaacagg tgtgattgaa tatcggtgcg gtctgcgcgt tttttgatgt cgcggacacg
      901 gacacgatta taggcaaatg tagaatacca accttcgggc aatttatccc atacgccgtt
      961 ccaatcgatt ttgcccaaaa tattgatgcc ggtaatccgc gcgctttggg cattgaggta
     1021 agccgggtcg cctttggctt cttctttgcc gtttttaatt tgcgcttcat aaccccggac
     1081 aatcaaatcg cggtaggcat tgttgaacca acttgcctcc aagttgccga aatcgccttt
     1141 aaatacgatg ccggcttctt tgttgaacga tttttccgga tcgattttga ccgccttgct
     1201 ttgaacaccc gcccgccagc cgtacatttc cgcaaacgag ggcaggcgga agccggttga
     1261 agtgcggtaa gtcaaatcca gccagtcggc aggtttgagg acgatgcctg cgttccagga
     1321 cagggtgcgg tgcgtgccgg tggaaacgct gctgtcgtcc gaatgcgtgc tgcggtagtc
     1381 gtagcgcaag cccgcgccga catccgccca cctgcccaaa cggacattgt cccgaactgc
     1441 cgcgtaatag cttttgccgt tgatgctgcg cggcgtgcag tccgtataag tattgttgcc
     1501 aaagaggcag atttgccccg taaccacatt tcccccgcct atgctgaccc aatagggttt
     1561 gtcttggctg ccgttggggc tgtttttgcc gttttgaggg ggcgtgttca acgaataggc
     1621 gcgtttggcg ttttgaaaat aataatcctg atggcggaga ttagagccaa agcggtcaaa
     1681 accgagattc acgctcaggt tgtggcggat tttggcggta tcgaaggatt ttttgaatgc
     1741 cgcctgcaag agcctgtggc tttccccgta aatcacgcga tcggatttgt agtaggaaaa
     1801 cggcttgtcg gcactcggac ggcaatattt gtccgaaccg tcggcagaac agtgcgtctg
     1861 ctgaaaatga ttgtccaaac cgatgccctg acggtcgtaa gagaggcggg cataatctgc
     1921 ccaagtgtct ttatcggcat tggtatagac atattccaaa ccgtagcggc ttttggtgtg
     1981 cgtctcgtcg taaaacacgc ccgtaccgta ttccgcgccc accagcgcac cgttttcacc
     2041 gttggtaaac agaccgccgt atttgtggtt gcccacgtat ttgccgttac cgggcaaaga
     2101 acccgcctgt tttgaatttg catcaaaaac cgccttggtc aggaatgccg gaaccgtcat
     2161 atcgcgcgtg tcgaaagttt gttgcgtgtg ttcgagtatg ccgccgatgt agtgccgttt
     2221 gttttcaaaa cgaaaacccg ggcgaaacag ccacgatcgg ctttcatatg aaagcgggtc
     2281 ggcgaggaag cggttggggc ccgtgtagtc tcgggtagaa accgtttgac gttcgtcttt
     2341 gccgacaaca tcttttttcg gtttgctttt acacgtttcg taatttttgc cttcgcattc
     2401 atcttcaacg atgaaatagg cgtattcgct gctgtcttca accggcacca gcctgttaaa
     2461 gctctgaacg ccgcgtcctg catcttcgtg ggcgcggatt tcccccgcgc gccgacccgt
     2521 gtggatcagc aaagcctccg caccgccgat gcgccccgcc agcgcgatgg attgggtaag
     2581 cccccggttt ttgccggaat aggcggtttt actctgaatg ccccactgcc tgccttcccc
     2641 gataacatcg tcggcggttt tggtttgaaa tgcgaccgag cccgccaatg cgccgctgcc
     2701 ttgttcgacc gagtttgagc ctttgctgat ttcgacagct ttgacgtttt catactcgat
     2761 ttcattgatt gcgccgctgc tgcccgccgt cctcgtcccg cccaatgccg cctgcgcggt
     2821 gtaggactgt atttgcgcca agccgtccac cgtcaaggaa acgcggtttt tgtccatacc
     2881 gcgtatcgag tagccagagc ttgcaccgcg cccctgttcg acaacggcga tgccggggtc
     2941 gtaacgcgtc aggtcgcgga tatcgagtac ctgttccttg ctgagggtgt cggcggtttt
     3001 gaccaatttg cccaaaccgg ttacttcgtt atcgcggcgg gttttctgtt ttttggcttt
     3061 tacctgtatg gtatccaact gtttttcctg tgcttgtccg gcttgcacat tttctgcata
     3121 aacgggcagc gcggtcatca gcgacaggca taaaatattt aatcggaaca aatgttgctg
     3181 ttgcatagtg tttccctaat cttcgctttc agacggcatc ggaaggagcg gtgccgtctg
     3241 aagccttatt cttgattgtt cggcaaccgt gcttattgca caggctgttg gcgtttcgca
     3301 ccgaatacca cggtcgcgct gcttgctgat tttccatcgc cggatgcaac cgttgccttt
     3361 tctgtttgtt tatcgcccgg ataggcaaac catccgccca actcttcggc tttgggcccg
     3421 taaaaaccgc cctgcacctt ggcatctgtg atatatgcct taggcgtgcg ggtgttatcg
     3481 ctttgatcga gatcaaaacc taagttatca gttttcgccg taccggaaaa accgttgccc
     3541 tcaatatcac cctcaatagt aaaggttgcc gcctgcctgt tgtcagcggt taacgtgccg
     3601 gtaatttttt tatcgccaaa attcacagta aattccgccc tgttgccgcc ctctttatct
     3661 gaagcattgc cgctccagct tgtgccgttg gcaatatgcc cgtaccaaga cccccgataa
     3721 acgatgtctt gttgctcttt tggaatctct ttttcatcgg tgcgctcgcc ttggaggaac
     3781 atactttgtg caccctgtgc cgtttgggcg gcggcttggc tgctgttgcc gcttcccacc
     3841 gtgttgccgg cggttttgcg cgtcagcaac ccgtatttca gataattgag gttggaacag
     3901 cagacttcga cttcataggt tttcgttcct gcctgcccac cggtaacgct ctcagtaccc
     3961 gaagcagttt gcgcgccgct agagcctgcc gcacctgttt gggctttggt atctttgtca
     4021 tcgtttttcg gcgtgtaggt cgttttatag ataaaggctg ttccgccgtt tttaccttga
     4081 tcgggctgat tattttcgcc tgttgaattt tgaggcaaga gcggaatcat aatgccgtcg
     4141 acaaccagtt gggcggcgtt gctgaagttg tcgagatttt tgattttctt gtcgtttagt
     4201 gtcaattcaa ccgcatccaa aaccgtggtc agcttaccgt tttcagacga cgtgcctgcc
     4261 gcaccgtttg atgctgccgc atctgtgccg cctgaagccg ccgcagtatt gccatttgcg
     4321 ggtttgtctt tggttttcgc gctgccgaca acggcaactt tttgatcgtc gctcaaaaag
     4381 cggaaaccca attcctcacc ctgcgggccg aaaaagccgc cgctcaaaga agacgaatcg
     4441 gaaacaaagg gatgttcctt ggtttcgctg ttttggggtt tgtcggttgc cgttgccttg
     4501 ccgttgaagc ggttgcctgt tacttgagcc tcaaggctgt agtattgcgt ggtggtggct
     4561 tggttgttat cggtattcgc attgttgcgt atcagtttgc ccgtcaattt tttattatgg
     4621 aaatccactt ctaaatttga ggtaaaacca taaccctctt gaccatctgt cagcgtggat
     4681 ttgtttttgt tggaatattc ttcgccgtca tcgcccgaaa atccgctata cctgtcgcct
     4741 tgactttttg aaggttggat aatttcacga aatttttgac ccttttttgt atcggtcgca
     4801 aaatgccaca cacctttata ggtaattttt ccagaagcgg gaagttgtcg ggaaggttct
     4861 ttaccgtgat agaagatata accgtcgtcg ccgttttttg cacttttagg ttccaccttt
     4921 aagttaaact ctcgtttggc gtgtttgtaa aaccagccgg aataaacata tttaaaattt
     4981 tcgtaatctt ttgcctgatt tttaggttgg tttataccgt tgccagtgtt gccgttttga
     5041 tggtttgatg gtttgagata gggggaagaa taaatattgt tgtcgctgtc tgtttctact
     5101 ttttcgataa ccgatttttg ccgtttaggg agttccttag gttcgtccgg caatcctgtc
     5161 gcctcccaat cactctcgtc cagtttaacc tcgtcttctt ttgcctgcgg ataccaattc
     5221 ctccgtttca acctcattgc aaaaccgtat ccgccttggt ctttttgggc ttgcggtttt
     5281 tcggaagaaa catcttgata ttttggcgcg ggacgcgggg cttcggtatc gacagaatca
     5341 agatcgaaac tgccgcctcc gcccaaacaa gcactcaaca aaaacacagg cagcaccata
     5401 gcagcctgat ttaccaatgg attgttcata taaacccaat tcaattaaag aatgataagg
     5461 attattattt tatttatttt taacaaattt gcaaatactt ttttattttt ttagggaata
     5521 caccaaaatc ccgtcatttc cgcgcaggcg ggaatccgaa cgcgtccgca cggaaaccta
     5581 tatcccgtca ttcccacgta tatcccgtca ttcccgcgca ggcgggaatc cagtgcgttg
     5641 agtttcagct atttagaata aattttgaaa ctctaatcgc gtcattccca cgaaagtggg
     5701 aatccaagac gcaaaatctc aa
//
LOCUS       DAXHTY010000104         5696 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXHTY010000104 DAXHTY010000000
VERSION     DAXHTY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.104"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            98..814
                     /locus_tag="ACFNMK_001770"
     CDS             98..814
                     /locus_tag="ACFNMK_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC6364980.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            963..1865
                     /locus_tag="ACFNMK_001771"
     CDS             963..1865
                     /locus_tag="ACFNMK_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC6364981.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1908..2681
                     /locus_tag="ACFNMK_001772"
     CDS             1908..2681
                     /locus_tag="ACFNMK_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC6364982.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEAGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2793..4481
                     /locus_tag="ACFNMK_001773"
     CDS             2793..4481
                     /locus_tag="ACFNMK_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC6364983.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4574..5530
                     /gene="gshB"
                     /locus_tag="ACFNMK_001774"
     CDS             4574..5530
                     /gene="gshB"
                     /locus_tag="ACFNMK_001774"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC6364984.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1317 a         1549 c         1582 g         1248 t
ORIGIN      
        1 aatttgtgca atgctttaat tcctctgaca cgatttgaaa cacagccgcc ttaaaacatc
       61 ttaaaaaatt tgaaagcgta aaaatacgga aaacatcatg aactttgaaa acgacgacat
      121 tatccatgcg ccgaccacgt cttccctgat tctcgaagag cggcacgatt cggagctgtt
      181 ccgtgtttac gccctgattt tggacggcat taccgatcag gtgctgctgc ccggcaaaaa
      241 gctgaccgaa tctgaacttt gccgtcagat ggtgtgttcg cgcaacaccg tccgcggcgc
      301 gctgtcgctt ttggcgcacg acaagattgt cgatttgcaa cccaacaggg gcgcgttcgt
      361 ccacgttccc gatttgaaag aaatgcagga tgtgttcaat gcgcgcatcg aaatggagac
      421 gatgattttg aatatcctcg cagatttgcc ggatttggaa acgcgcctca agccgcttta
      481 tgcgatgata cggcgcgaag aagaggcctc cggcaggggc gaccgcgttg gctggaaccg
      541 cctgtccaat gccttccacg tcgaactggc gcgcctggtg ggcaacgatg tgctgttcga
      601 cattatgaac acgctgtgcg cgcggtcttc cctgattgtc gccgtggcgg gcgtgcatcg
      661 cgaggaaaaa cacgccatca atacgcatac gcattccgaa caccgcgaaa tcctcgacct
      721 gctgctggcg ggcaggcgca accgcgtggt caaaatcctg cgccgccatt tgggcaactg
      781 tatggagcgt ttggaaaaga ctttggaaga ttgaatgccg gagcggataa gccgcctgaa
      841 ccttcaggcg gttttttaat ggcgaacctg atgccgtctg aaatatggat gcgggtatct
      901 gcaattttca gacggcaatt tttaaaccgc atatatcatg cggcaataaa ggaggtcagg
      961 agatggacag cctgatgacg ttgctttcgg tattgatacc gatgtttgcc ggatttttta
     1021 tccgtgtgcc caagccttac ctgcccgctt tggataaggt gctatcggtc ttggtgtatg
     1081 ctgtgctgct gctgatcggc gtctcgttgt cgcgcgtgga ggatttgggt tcgcggttgg
     1141 acgatatggc gttgacggtt ctgtggctgt ttgtttgtac ggtcggggcg aacctgcttg
     1201 ctttggcagt gttgggaaag ttattcccgt ggcggataaa ggggaaaggg aagggcgttt
     1261 cggtcggcgt gtcgggcagt gtggggcagc tcggatgcgt gctgctcgga tttgcattcg
     1321 gcaaactgat gcgcgatatt tggatgccgt ctgaaagcgc gggcatgtat tgtctgatgc
     1381 tgctggtgtt cctcatcggc gtacagctca aaagcagcgg cgtatcgttg cggcaggttt
     1441 tggtcaaccg caggggtatt cggttgtcgg tctggtttat gctttcatct ctttcgggcg
     1501 ggctgctgtt tgccgcatcg gcagacggcg tgtcgtggac gaaaggtttg gcgatggctt
     1561 ccggcttcgg ttggtattcc ctctcgggtt tggtcatgac cgaggcttac ggcgcggtat
     1621 ggggcagcat catgctgctg aacgatttgg cacgagagct gtttgcactg gcatttatcc
     1681 cgctgctgat gaagcgtttt ccggatgcgg cggtgggggt tggcggtgcg accagtatgg
     1741 attttacatt gcccgtgatt cagggtgcgg gcggtttgga agtcgtgccg gtagcggtca
     1801 gcttcggcgt ggtggtcaat atcgccgccc cgtttctgat ggtggtgttt tccgctttgg
     1861 gttgaacgcg gtaaaatcgg catcccgatg caaggaggca gaaaacgatg aaaccgaaaa
     1921 tccaaaggca cggagagatt ttaagccttg tccgccggca tcagtttatg tcggtggacg
     1981 agcttgccgc cgcattggac gttaccccgc agacgatacg ccgcgacatc cgcgagttgg
     2041 aggaggccgg cagcctgaaa cgccatcacg gcggcgcatc ttcgggcgga aacttgccgg
     2101 agggcctgcc cgccgaccgc caaacccggt gtcaaaacga aaaaaacgcc attgcccggc
     2161 tgattgcgga acacatcccc gacggttcgt cgctgtttgt cagtatcggc acgacgatgg
     2221 aagccgtggc atcggagctg gtcaggcggc gcggcagcct gcggattatt accaacaata
     2281 tccacgtcgc ctccatcgtt tcggcgcgga cggattacac ggtcattatc acttccggag
     2341 tcgtccgccc tttggacggc ggtattaccg gcgtggcaac cgtcgatttt atcaaccagt
     2401 tcaaagtcga ttatgccgtg atgagtacgc acggcgtgga aagcgacggt tcgcttttgg
     2461 acgacgacta caaagaagtc agcgtgatgc aggcgatgat tgccaatgcg cgcgtccgct
     2521 tcctcggcgt ggaccacagt aaattccgca gcaacgcgct ggtcaggctc ggcgacatta
     2581 cggcgtttga caaagtattt accgaccggc tgcccgatac cgcgatgcag aagatgctga
     2641 aagaggcggg ggtggaatgc ctgattgccg atgccgtctg aacgctatgt caaagcgcgc
     2701 aagtcgggta caataaacac atcatcaaac cgcttcagac ggcataagga accgccccaa
     2761 tgccgtctga agccatctgt tcaaagaaaa ctatgctcaa taaagaccaa tttgcggaca
     2821 accattttat ccgcaccttc atcgaagaag acctcaaaag cggcaaacac gaagccgtcc
     2881 aaacccgttt tccgccggag cccaacggct acctgcacat cggacacgcc aaatccatct
     2941 gcctgaactt cggtttggcg tatatttacg acggcttgtg caacctgcgt tttgacgata
     3001 ccaaccccga aaaagaaaac gacgaatacg tcaacgccat caaagaagac gttgagtggc
     3061 tcggtttcca ttgggcgggc gagccgcgtt tcgcttccaa ctatttcgac cagctttatg
     3121 actacgccgt cggtttggtt aaagacggca aagcgtatgt cgatgatttg acgccagaag
     3181 aaatgcgcga ataccgcggt acgctgaccg aagcaggcaa aaacagccct tatcgcgacc
     3241 gcagtatcga agaaaacctc gacctgttca cgcgcatgaa aaacggtgag ttccccgacg
     3301 gcagcaaaac cctgcgcctg aaaatcgaca tggcatccgg caacatcaat atgcgcgatc
     3361 cggtcatcta ccgcatccgc cgcgcccatc accacaacac cggcgacaaa tggtgcatct
     3421 atccgatgta cgactacacg cattgtattt ccgatgccat cgaaggcatc acgcattcct
     3481 tgtgtacgct cgaatttgaa gcccatcgtc cgctgtatga ctgggtgttg gacaacatcc
     3541 ccgcgccgca cgccacacgt ccgcgccaat acgagttttc acgcttggag cttttgtaca
     3601 ccattacctc caaacggaaa ttgaatcagt tggttgtgga aaaacacgtt tccggctggg
     3661 acgatccgcg tatgccgacc atttccggta tgcgccgacg cggctacacg cccgaagggc
     3721 tgcgcctgtt tgccaaacgc gccggtattt ccaaatctga aaacatcgtc gatatgagcg
     3781 tgttggaagg cgcgattcgc gaagagttgg aaaattccgc cccacgcctg atggcggtgt
     3841 tgaacccgct caaagtaacc ctgaccaact ttgaaaccgg cagaacccaa agccgccgtg
     3901 ccgcgttcca tccgaaccac gaagaaatgg gcgatcgcga agtacctgtt tcacaaacca
     3961 tttacatcga agccgacgac tttgccgaaa atccgcccaa aggcttcaaa cgcctgattc
     4021 ccggcggcga agtacgcttg cgccacggct atgtcatcaa gtgcgatgaa gtcgtcaaag
     4081 acgaagcagg caatgtggtt gaactcaaat gcagcatcga ccacgacacc ttgggcaaaa
     4141 atccagaagg ccgtaaagtt aaaggcgtga ttcattgggt ttccgccgaa cacgccgccg
     4201 aaatcaaagt ccgtctgtac gaccgcctct ttaccgtcga gcgtcccgat gccgtgcgcg
     4261 gcgaagacgg cgaatacctg ccgttcaccg atttcctcaa tccggaatcc gtcaaggaaa
     4321 tcaccgccta cgccgaacct gccgccaaag atttgccggc agaaagccgt tggcagttcg
     4381 agcgtatcgg ctacttcgta accgaccgta aagaccacgg caaagacacg ccggtgttta
     4441 accgcacggt cacgctgaaa gattcttggc agcctaagta aaaccccatc cttgccgtct
     4501 gaatattgtt cggacggcat ttcccctttg cccgcgaaat gcggcacatt cggcacaccg
     4561 gaaaggaaat atgatgaaag tcctctttat cgccgacccg atggcaagtt tcaaaaccta
     4621 caaagacacc acctacgcca tgatgcgcga gatggcaaaa cgcggctggc ggctgtttca
     4681 taccttgagc agcggattgt cggttaaaaa cggtctggtg gttgctgatg cgtctgcttt
     4741 tgagtttctc ggcgcacaag acgaccacga caaaaactgg tttgccgccg ccgacaaagt
     4801 tcagacggca ttgaaagcat ttgatgccgt gattatgcgt accgatccgc cgttcgatat
     4861 gcaatacctc tacgccacac agcttctgac gctggcggag cagcagggcg cgaaggtctt
     4921 taacagcgga caggcgatgc gtgatttcaa tgaaaaactg gcgattttga acttcagccg
     4981 ctttaccgcg cccacgctgg taacgacccg ttccgccgat gtccgcgcat ttttgaaaga
     5041 acacggcgac atcatcatta aaccgctcga cggcatgggc ggtatgggca tcttccgcct
     5101 gaccgaaaaa gaccccaaca tcggcagcat cctcgaaacc ctgatgcggc ttgattcccg
     5161 caccattatg gcgcaacgct acattcccga aatcgtacac ggtgacaaac gcatcctgat
     5221 tatcggcggc gaagtcgtcc cctatgcttt ggcgcgtatc ccgcaaaacg gcgaaacacg
     5281 cggcaacctt gcggcaggcg gacgcggcgt ggcgcaggaa ttggacggac gcgaccgcga
     5341 aattgccgaa acgctcgccc ccgaactcaa acggcgcggc atcctgctgg ccggtttgga
     5401 cgttatcggc agcaacctga ccgaagtcaa cgtaaccagc ccgaccggat tccaagaaat
     5461 tatgaaacaa aaaggtttcg acgtggcggc aatgtttgcc gatgccgttg ccgcgtggtc
     5521 ggtacgttaa accgatgccg tttgaaaggc ttttgcttca caaccgtttg gtttgttcgg
     5581 gcaggcaggg ttttccctca gccggcaggg atgcggttaa tgccgtacgg acaccccgcc
     5641 gccccgtttt cagacggtat atatagtgga ttaacaaaaa tcaggacaag gcgacg
//
LOCUS       DAXHTY010000105         5502 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXHTY010000105 DAXHTY010000000
VERSION     DAXHTY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.105"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(254..2050)
                     /locus_tag="ACFNMK_001775"
     CDS             complement(254..2050)
                     /locus_tag="ACFNMK_001775"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC6364985.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFIH
                     DPDYVSETAAEKLARVRAVMTEKVADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGIAVEPYAQVAGKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2106..2447
                     /locus_tag="ACFNMK_001776"
     CDS             2106..2447
                     /locus_tag="ACFNMK_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364986.1"
                     /translation="MPSEKHNHLIKSPACCKTDGLIGWCVFARKKRCKVVCCKILGIK
                     CRFMPFFGSNMENIVKLEHEVLIVCVGRYLDYVTIRAFIKISLPGGILFLKTVGFGFV
                     WGQSRPNWSNR"
     gene            2508..3761
                     /locus_tag="ACFNMK_001777"
     CDS             2508..3761
                     /locus_tag="ACFNMK_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6364987.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3767..3919
                     /locus_tag="ACFNMK_001778"
     CDS             3767..3919
                     /locus_tag="ACFNMK_001778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364988.1"
                     /translation="MPSETANRFQTALPRLWKKTPTSRKIRANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            3916..5427
                     /gene="lysS"
                     /locus_tag="ACFNMK_001779"
     CDS             3916..5427
                     /gene="lysS"
                     /locus_tag="ACFNMK_001779"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC6364989.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKIAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIVNEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSLVIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
BASE COUNT         1199 a         1405 c         1603 g         1295 t
ORIGIN      
        1 cggaatatag cggattaaca aaaaccagta cggcgttgtc gcgccttagc tcaaagagaa
       61 cgattctctc aggtgctgaa gcaccgagtg aatcggttcc gtactattcg tactgtctgc
      121 ggcttcgtcg ccttgtcctg atttttgtta atccactata tttgcttgcg gcaagatgaa
      181 aacggtgcgg gatgttttgg gaaaccaatg ccgtctgaag ggctttcaga cggcattttt
      241 tgcgccgtgc tgtttaacgc gccagcggtt cggtgcgttt gatcagccac gcttttgccg
      301 cgccttcggt cagcggctcg aggcggcggc ggacttcggc gtggtagcgg ttgacccagt
      361 cgatttcgcc gtcggtcatg agggcggtgt ccatcaggcg ggtgtcgatg gggcagaggg
      421 tcagggtttc aaaacagagg aagctgccga attcggtttc ttgaggggcg gcgacggctt
      481 ggttggcggc aaggttttca atgcggatgc cccattttcc cgggcggtag agtccgggtt
      541 cgatggaggt aaccatgcct tttttcatgg cggtttcggg cgtggcgggg gcggcgaagg
      601 cgatgcgctg cgggccttcg tggacgttga ggaaatagcc tacgccgtgt ccggtgccgt
      661 ggccgtagtc gcattgcgcc tgccacaggg gtttgcggca aatcgcatca atcagcggcg
      721 aggggatgtt ttctgggaac acggcttcgg caagcgcgat atgggctttg agaacgaggg
      781 tgttgtcgcg tttttgttcg gcggtgggtg tgccgacagg gacgacgcgg gtgatgtcgg
      841 tcgtgccgcc tttgtattgc gcgccggagt cgatgagcag cagcccgttg ccgctgatgg
      901 tgctgtggct ttcgggcgtt gcgctgtaat gcggcagggc gccgttggcg ttgaagcctg
      961 cgatggtgtc gaaactcaat gacacaaaac ccgggcgcac gctgcggtgg cggtaaagca
     1021 tggtgtccac gtcgatttcg gtcaggctgc cgccgttgcc gatgatgtct tcaaactcgg
     1081 cgaagaaacc gcacaacgcc gcgccgtcgt gttccatcgc ttcgcggatg cgggcgatgt
     1141 cggcttcgga tttgcaggat ttgaacagcg tggacgggtt gattccctcg ataaggcgca
     1201 cgctttcggg caggcgcaca agcgtgctga cggcggtttt gttcggttcg ataagcagag
     1261 cgccgccgat ttgcgcgagt ttgccggcaa cttgggcgta aggctcgacc gcgatgccgg
     1321 cggtttgcag cgcggcggcg gcttcggcgt tcagacggca tcggtcggta aacaggacgg
     1381 cgttgtcttt gccaatcagc aggaaggaca cgaaaacagg gttgaaaggc acgtcgctgc
     1441 cgcgcaggtt ggtcagccaa gcgatgtcgt caagcgagga aaccaagtgg taatccgcga
     1501 ctttttccgt catcacggcg cgcacgcggg cgagtttttc ggcggcggtt tcagaaacat
     1561 agtcggggtc gtggatgaat accgtttcgg caggaatggc cgggcggctt gtccacactt
     1621 ggtcaagcag atcgtcgggg tgttcgatgc ggatgttttt ggcggtcagg gattgcgcca
     1681 aagtgcgttt gccggtgagc gagaccatat cggaagggat gccgacggcg gcgttttcgg
     1741 gcaggtttgc cgcgagccat tcgttatacg gcggcacttg cccgcttttt tgcagctcaa
     1801 tgccgctgcc ggaaagctgt ttggcggctt gttcccaata gcggctgtcc acccatacgc
     1861 ccgcttcatc ggtggtcagg acaaacgtgc cgaccgagcc ggtaaagccc gacaattcgc
     1921 ggcgcgcctg ccaatgctcg ggcaggtatt cggacaggtg ggggtcggcg gaagggatga
     1981 cgagcgcgtc caagccttgc gccttcatgg cttcgcgcaa tgcggacagg taattcgata
     2041 cggtattcat aacagttcct ccaatcgggt tttgcggctt cagacggcat ggacggaaat
     2101 ctgaaatgcc gtctgaaaaa cataaccatc ttatcaaatc gccagcctgc tgcaaaacgg
     2161 acgggctgat cggatggtgc gtgtttgcgc gaaaaaagcg atgtaaggtt gtttgttgta
     2221 aaattttagg cataaaatgt cgtttcatgc ctttttttgg aagtaatatg gaaaatatag
     2281 tcaagttgga gcatgaagtt ctaatagtgt gtgttggcag gtatttggat tacgttacaa
     2341 tacgcgcttt catcaaaatt tccctaccgg gcggtatcct gtttttgaag acggtaggat
     2401 ttgggtttgt ttgggggcaa tcccgcccaa attggagcaa tagatagggc tgtgtccgaa
     2461 tatgcggctc tgtgtgttga aacacggtta aaaatacgga agactttatg tccgaacaac
     2521 atatttcgac ttggaaaagt aaaatcaacg cattgggtcc ggggatcatg atggcttcgg
     2581 cggcggtcgg cggttcgcac ctgattgcct cgacgcaggc gggcgcgctt tacggctggc
     2641 agatcgcgct catcatcatc ctgaccaacc tcttcaaata cccgtttttc cgcttcagcg
     2701 cgcattacac gctggacacg ggcaagagcc tgattgaagg ttatgccgag aaaagccgcg
     2761 tttatttgtg ggtattcctg attttgtgca tcctctccgc cacgattaac gcgggcgcgg
     2821 tcgccattgt aaccgccgcc atcgtcaaaa tggcgattcc ctcgctgatg tttgatgccg
     2881 gcacggttgc cgccttgatt atggcatcct gcctgattat tttggtgagt ggacgttacc
     2941 gcgctttgga tcgcgtttcc aaaatcatca tcgttacttt gagtatcgcc acgcttgccg
     3001 ccgccggcat cgctatgtcg cgcggtatgc agatgcagcc cgattttatc gagccgacac
     3061 cgtggacgct tgccggtttg ggcttcctga tcgcgctgat gggctggatg cccgcgccga
     3121 ttgaaatttc cgccatcaat tctttgtggg taaccgaaaa acaacgcatc aatccttccg
     3181 aataccgcga cgggattttt gatttcaacg tcggttatat cgccagtgcg gttttggctt
     3241 tggttttcct tgcactgggc gcgtttgtgc aatacggcaa cggcgaagca gtgcagatgg
     3301 cgggcggcaa atatatcggg caattgatca atatgtacgc cgttaccatc ggcggctggt
     3361 cgcgcccgct ggtggcgttt atcgcgtttg cctgcatgta cggcacgacg attaccgttg
     3421 tggacggcta tgcccgtgcc attgccgaac ccgtgcgcct gctgcgcgga aaagacaaaa
     3481 cgggcaacgc cgaattcttt gcctggaata tttgggtggc gggcagcggt ttggcggtga
     3541 ttttttggtt tgacggcgta atggcgaatc tgctcaaatt tgcgatgatt gctgcttttg
     3601 tgtccgcccc tgtgtttgcc tggctgaatt accgtttggt caaaggtgat gaaaaacaca
     3661 aactcacatc aggtatgaat gcccttgcat tggcaggctt gatttatctg accggtttta
     3721 ccgttttgtt cttattgaat ttggcgggaa tgttcaaatg ataacgatgc cgtctgaaac
     3781 cgcaaaccgc tttcagacgg cattgccgcg tttatggaaa aaaacaccga catcgcgtaa
     3841 aatacgcgca aattttgtga ttcggtcagg tcgtctgaaa cagattgcgc ctgatttatt
     3901 ttttcggaaa accctatgag cgaacaaaac catccgcaaa ccgagccgca gttggacgaa
     3961 aaccaaatca tcgccctgcg ccgcgaaaaa ctgcacaaca tccgccaaca gcgcaacgcc
     4021 tatcccaacg acttcaaacg cgacagcttc gccgccgatt tgcacgccca atacggcgaa
     4081 atcggcaaag aagaactcga cccacaaggc attcccgtca aaattgccgg ccgcatgatg
     4141 ctcaaacggc aaatgggcaa ggcgagtttt gccaccattc aagacgtgtc cgggcaaatc
     4201 cagctttatc tgaacaacaa aggcgtgagc caagaagttt tggacgactt caaccattgg
     4261 gatttgggcg acatcgtcgg cgcggaaggc actttgttca aaaccaacca cggcgaactg
     4321 accgtacgcg tgtccggcat ccgcctgctg tccaaatccc tgcgtccgct gcccgacaaa
     4381 cataaaggtt tgagcgatca ggaaaccaaa taccgccaac gctatgttga tttgattgtc
     4441 aatgaagaat cgcgcaatac ctttatcaaa cgcagccaaa tcatccaatc cgtgcgtaat
     4501 tttatggtgg gcgagcatta tctcgaagtc gaaaccccga tgatgcaccc gattcccggc
     4561 ggcgcgacgg caaaaccctt cgttacccat cacaatgcct tagatattcc gctttacctg
     4621 cgtatcgcgc ctgagctgta tttgaaacgc ctggttgtcg gcggcttgga acgcgtgttt
     4681 gaaatcaacc gcagcttccg caacgaaggt atgtctgtgc gccacaatcc cgaattcacc
     4741 atgattgaat tctacgaagc cttctccgac tacgaacgca tgatgcagat ggcggaagac
     4801 atcatcagaa acgcatcgcg cacagtaaac ggtacggcaa acatcactta caacggcaaa
     4861 gaagtcgatt tggaaagccc gtttgagcgt ctgaccatcc tcgaagccat caaaaaatac
     4921 aatccgcact acaccgacga gcagttgaac gatgcggaat ggctgaaaaa agaaatcgtc
     4981 aaacacggcg aaagcctgcc gccgtccctg gtcatcggca gcctgcaact cgcgctgttt
     5041 gaaggttgcg ccgagggcaa gctgtggaac ccgaccttca tcgtcgatta cccggtcgaa
     5101 gtttcaccgt tggcgcgcgc ttcggatacc aaacaaggtc tgaccgaacg tttcgaattg
     5161 ttcgttgtcg gccgcgaact ggcaaacggc tattccgagt tgaacgaccc cgaagaccaa
     5221 gccgaacgct tcaaagcgca agtggcgcaa aaagacgcgg gcgacgacga agcgatgcac
     5281 tacgatgccg actacatccg cgcgatggag ttcggtctgc cgccgacagg cggttgcggc
     5341 atcggtatcg accgcttagt gatgctgctg accgattcgc aaaccatccg cgatgtgatt
     5401 ctgtttccgc agatgcgtcc cgagtaatca taaaaacagt tgaaatagaa atgccgtctg
     5461 aacccgattg gattcagacg gcattttgta tggcaggatt ga
//
LOCUS       DAXHTY010000106         5433 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXHTY010000106 DAXHTY010000000
VERSION     DAXHTY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.106"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..183)
                     /locus_tag="ACFNMK_001780"
     CDS             complement(<1..183)
                     /locus_tag="ACFNMK_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6364990.1"
                     /translation="MRKSRLSQYKQNKLIELFVAGVTARTAVELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDG"
     gene            complement(326..1681)
                     /locus_tag="ACFNMK_001781"
     CDS             complement(326..1681)
                     /locus_tag="ACFNMK_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC6364991.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1696..1968)
                     /locus_tag="ACFNMK_001782"
     CDS             complement(1696..1968)
                     /locus_tag="ACFNMK_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC6364992.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(2094..2831)
                     /locus_tag="ACFNMK_001783"
     CDS             complement(2094..2831)
                     /locus_tag="ACFNMK_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC6364993.1"
                     /translation="MKKLSRIVFSIVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPVKILTWDENGRLLSELSIRHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(3112..4011)
                     /gene="prmB"
                     /locus_tag="ACFNMK_001784"
     CDS             complement(3112..4011)
                     /gene="prmB"
                     /locus_tag="ACFNMK_001784"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6364994.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIVPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            4156..4749
                     /locus_tag="ACFNMK_001785"
     CDS             4156..4749
                     /locus_tag="ACFNMK_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6364995.1"
                     /translation="MADGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHATSKANQAELSIITKLDDLLRYAKYEGSYSDKKGHPDIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            4861..>5433
                     /locus_tag="ACFNMK_001786"
     CDS             4861..>5433
                     /locus_tag="ACFNMK_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC6364996.1"
                     /translation="MTKLYAEIAKMETQDDGTVKVWGYASSEAVDSDGEVIAAAAMKA
                     AIPDYMKFGAEREMHGSNAAGTAIEINVEDDGRTFFVAHIVDPVAVTKVKTGVYKDFS
                     IGGSVTAHDELNKSQITGLKLTEISLVERPANPDAVSTCFKADKGAETVNNDTEHNAT
                     YFSHFPSKQKSTDSGRCPFLYRFPYFFTAGS"
BASE COUNT         1497 a         1384 c         1195 g         1357 t
ORIGIN      
        1 gccgtcaaac atttccaaat gcggactgtt ttgataaata agtaatcgta aacgatgaaa
       61 ataataggct gcggtatttt tattaacgcc tactaactct actgccgttc ttgcagttac
      121 acctgcgaca aacagttcaa tgagtttatt ttgtttatac tggcttagac gactttttct
      181 cataggtata attctaactt aatttgaatt tccctagtta tctaggacag ccccatattc
      241 ttttctaaag acattttacc cgatgcctat aaacaaaaat gccgtctgaa gcctttcaga
      301 cggcatttcc ccatcaaaac cgcaatcagt ttttcatcga ttgaaccggt gcgggaatcc
      361 tgccgccccg gttgacgaac acttcgcatg agccttcttt gaccggcatt acaggcgcgt
      421 agcccaacag gccgccgaac tcgacgctgt cgccgacggt tttaccggtt accggaataa
      481 tgcgcacggc agtggttttg ctgttgatca tgccgatggc ggcttcgtcg gcaatgatgc
      541 cggaaatggt gtgcgcgggt gtgtcgccgg gaacggcaat catatccaag ccgaccgaac
      601 aaacggcggt catcgcttcg agtttatcca acgtcagcac gcctgcttcg gcggcggcaa
      661 tcataccttc gtcttcggaa acggggataa acgcgccact caaaccgcca accgcgctcg
      721 aagccatcat gccgcccttt ttcacggcat cgttcagcaa tgccaaagct gctgttgtgc
      781 cgtgcgtacc gcagacgctc aaacccattt cttcaagaat gcgcgccacc gagtcgccga
      841 cggcaggggt cggtgccagc gacaagtcga gaataccaaa cgggatattc agcatttttg
      901 aggcttcgcg gccgatgagt tcgcccacgc gggtaatttt gaaggcggtt ttcttcacaa
      961 cttcggcaac ttcggtcaat gtcgttgcat ccgaattttc caacgcggct tttacgacac
     1021 ctgggccgga tacgccgaca ttaatcacag catccgcttc gcccgatccg tgaaacgcac
     1081 ccgccataaa cggattgtct tccaccgcat tgcagaacac gacgattttc gcgcagccga
     1141 aaccttcggg cgtaatttca gccgtgcgtt tgatggtttc gcctgccagc ttgaccgcat
     1201 ccatattgat accggcgcgc gtactgccga cattgatgga gctgcacacg atgtcggtgg
     1261 tcttcatcgc ttcgggaatg gaacggatta acacctcgtc tgaaggcgac atgccttttt
     1321 gcaccaacgc ggaaaagccg ccgataaagg acacgccgat ggctttggca gccttatcca
     1381 aagtttgcgc cacgctgaca taggaatcgg catgggtagc cgccgcgatt tgggcaatcg
     1441 gcgtaacgga aatgcgctga ttcacaatcg gcacgccgta tttggcagac agatattttg
     1501 ccgtcgccac caagtctttg ccgaccgtgg taattttgtt gtaaatgttt tggtttaaca
     1561 cgtcgatgtc ggtgctgatg cagtcgtgca aatcaatgcc gatggtaatg gtgcggacat
     1621 cgaaattctg gtcggcaacc attttgacgg tttctaaaat ttcgccggat tggatactca
     1681 tcacattcct ccgactcaaa tgcggtgcat cgcttggaag atttcttcat tttgcatacg
     1741 gatatcaagc gcgagttttt tgctctcttc cgcaaacaaa tccaaaacct cctgccgaga
     1801 tttggggcat ttcgaggtat ccaccaagat aatcatggta aaaaaatcat ccatcagctg
     1861 ttggctgata ttaaggatgt tgattcggtt ttccgccaaa attttggaaa catcgtacac
     1921 gatgccgacg cggtctttgc cgatgacggt gatgactgaa ttgttcacag gcttactcct
     1981 tgcagatatc cgttaaagtc cgaaattata ccaccgttgg attttgaaga aatattgtca
     2041 acaatatata catacaaaat gccgtctgaa actatttcag acagcatcaa gattcagggt
     2101 tcgattaaat aaccatcctt atcccactgg gttttcctga ccaacttatc atcctgataa
     2161 acagcctcgc tctttttaga accatcttca taccactcca agaccacccc gttgcgttta
     2221 tggtggcgga tagacagctc cgagagtaat cggccgtttt catcccaagt gagaatcttg
     2281 acaggttcat cgttgaccat aaccatttcc gtcttgatac tgccgtcggc ataccattgc
     2341 ttccatacgc cgtttgcctt attttgctta aactggattt cgctttcctt accgccgtta
     2401 cggtaataac ggtatcccgt accttcactc aaaccatttt tataaggcat aaccgcagat
     2461 tttttaccgt tcggatacca gttgacccac tccccgtccg gtttaccctt gctgaagccc
     2521 cccgccattt ttttctgacc attaaaatgc cacaaaatca acataccgtt ttgcagggta
     2581 ggcacaaaag atttgatttg cgttgaagca acgatataag gttcggaata tttcttcatc
     2641 gacggataat aaaaatcctg cgcgtgtgcg atacccgccg ccacactata ttgcctgata
     2701 taagcggcag aagacatcgt cgccgtcagt ttcccgttct gattaaaata aacagaatag
     2761 gtctgcgccg gcaaagcggc cgaaaaaccc aacagtacga ttgaaaatac aatccgagat
     2821 aattttttca ttgcaatagc gatataaaaa caaggctgtg ttttagtaat ctgttgattt
     2881 caattatttg caagggaaaa gacaattatt ttccggttag gaataaacct attctattga
     2941 atagattgaa gccaagtacg cctatcaaca ctatattaaa acactgccaa aaacaattaa
     3001 cttataaaca atatggtaag gatttctctg ccaagcatca aacccgagac aacgtatcgt
     3061 aaaaatgccg tctgaaaaca aatcgtcttc agacggcatt tccccttcaa ctcactcttc
     3121 acccaataac tgctcgcgcg tcaagaggaa aacaaaaccg tcgcccccgc tggtttccaa
     3181 ccaagtaaaa ggcaactccg gatacgctgc ttccaataca tccctgttat gcccgatttc
     3241 caccagcaat acacctttgg gattcagaaa ctttgccgca ttcagaagaa tctgcctggt
     3301 ggcatccaac ccgtccgccc cgctgcccaa tgccaattcc ggttcgtgca aatactcttc
     3361 aggcaataac tcaaccgatt ccgcatccac ataaggcgga ttggaaacaa tcaaatcata
     3421 agtgccttcc aatccttcaa acaaatccgt atgaataagc cggatgcgtt cttccaaacc
     3481 ataatcttcg acattgatcc ctgccacttc caaagcatcc aagctcacat caaccgcatc
     3541 aatttgggca tcaggataat gatgcgccat ctgaatggca aggcatccgc ttccagtgca
     3601 aagatccaaa gcattatgca ccagctcatc gtattctatc caaggacgaa gtccgtcacc
     3661 cagcaattca taaataaaag agcgcggcac aatcacgcgt tcatctacat aaaaatcaaa
     3721 ctctccctgc catgcctggt gtgtcaaata agcggctgga atgtgttcga cagcacgacg
     3781 ctcaataacc gccagcactt cctctttttc agcttccaag agttttgcat caagatatgg
     3841 ggcaagcata tccaaaggca aattcaaagt atgcagaatc aaataagccg cttcatcatg
     3901 cgcattatct gttccatgac caaaaaagag ccctgcctca ttaaaacggc tgactgcaaa
     3961 acgtaaaata tcgcggatag tcgtcaattc ttgtgctgcc tgattaaaca taatatgaac
     4021 cattctgcgt atagatactt ttaattataa cagaaacaac aagcaaacct tttcatatcg
     4081 ccaaataacc acccaatcta cccatacaac tacataaatg cccgcgcgaa aaccatcgcc
     4141 cgaacggaaa cgacaatggc cgacggtatg ggcaatctga ttggctggga aaaaacgggg
     4201 cttgttgtcg gtaagcagtg gataaccgca aaagacgaca aggtatccga tgtctgcaat
     4261 gccaacggcg agatgggcgt aatcgggctt tacgagcctt tctcacacgg cgcattgacg
     4321 atacctggtc atccgaactg ccgatgcgag gttgtttccg tatcgggtgg cgaattgggg
     4381 gaatttgccg aaaaaaagga gcttcgtaaa gcggctatgc agtatgcgcg ggataacttt
     4441 atcggcaaaa gctatgtcaa tgaaaacagc gggcatgaac tgaaggtaac ttggcaaggt
     4501 gtgaaacacg ctacgtcaaa ggcaaatcag gcggaattat ccatcataac aaaacttgat
     4561 gacttattgc gctacgcaaa atatgagggt tcttattcgg ataagaaagg tcatcctgat
     4621 attattgcag cacataagta tcgtgccgtt gccaaggttg ggaatgagtc tttaaatatc
     4681 ggtgtgattg taagggaatt tccagacgac cataaacatt acgaccattt catcttgaag
     4741 gatgaataaa gcccttttgc agtgtcgttc tggagcggat agcgttaagg caagtacact
     4801 tccagccttg aaaaagggct ttaaattcag catgccattt atacaggcag gagtaaaccc
     4861 atgacaaagt tatacgcaga aatcgccaag atggagacgc aggacgacgg cacggtcaag
     4921 gtttggggtt acgcttcaag cgaggcggtc gattccgacg gcgaagtcat cgcggcggca
     4981 gctatgaagg cggcgattcc cgattatatg aagtttggcg cggagcgcga gatgcacggc
     5041 tcaaacgctg cgggaacggc aattgaaatc aacgtggaag atgacggcag aacctttttc
     5101 gtggcgcata tcgtcgatcc cgttgccgtg acgaaggtca aaacaggcgt ttacaaggac
     5161 ttttccatcg gcggcagcgt taccgcccac gatgagttga acaagtcgca aatcacgggt
     5221 ttgaagctga cggaaatcag ccttgtcgag cgccctgcca atcccgatgc ggtgtctacc
     5281 tgctttaagg cggacaaagg tgcggaaacg gtaaacaacg atacagaaca taatgctaca
     5341 tattttagcc atttcccttc caaacaaaaa agcaccgaca gcggccgatg ccccttcctt
     5401 tacaggttcc cctatttttt cactgcgggc agc
//
LOCUS       DAXHTY010000107         5375 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXHTY010000107 DAXHTY010000000
VERSION     DAXHTY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.107"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(159..2993)
                     /gene="lbpA"
                     /locus_tag="ACFNMK_001787"
                     /gene_synonym="tbpA"
     CDS             complement(159..2993)
                     /gene="lbpA"
                     /locus_tag="ACFNMK_001787"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC6364997.1"
                     /translation="MNKKHGFQLTLTALAIATAFPAYAAQAGGATPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIQNPNKKAEYFLAEGERES
                     KPVAKLAGNGNYLKNQLNRWVEERRSKGQALNSEEEAMVQEAQARHENLSAQSYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEANKF
                     RNKSGVYDGDDFRDGLYFVPNIEEWKGDTNLVKGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISEYS
                     EYTADGKDKYLLGKPEVIEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTQLSDSSGRLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2990..5194)
                     /locus_tag="ACFNMK_001788"
     CDS             complement(2990..5194)
                     /locus_tag="ACFNMK_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6364998.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAGPSVETTPVNQPAVGAAMRLLRRNIATSDKDGKDFPNSKQAEEKLSFKEG
                     DVLFLYGSKKDQRQQLEDKIHQLNPNVQIRTSKNENKKYGYKFVDAGYVYTKDGKDEI
                     EWTSNYKQSTNRFGYDGFVYYSGEHPSQSLPSAGTVKYSGNWQYMTDAIRHRTGKAGD
                     PSEDLGYIVYYGQNVGATSYAATADDREGEGKHPAEYTVDFDQKTLNGKLIKNQYVQR
                     KTDEKKPLTIYDITAKLDGNRFTGSAKVNTEVKTNHADKEYLFFHTDADQRLEGGFFG
                     DNGEELAGRFISNDNSVFGVFAGKQKTEAANAADTKPALPSGKHTKILDSLKISVDEA
                     TDDHARKFAISTMPDFGHPDKLLVEGREIPLVSQEKIIELADGRKMTVRACCDFLTYV
                     KLGRIKTERPAVQPKAQDEEGDEEDTGVDSVEEGEDEIDDEDEVSEEDAEEIEEEADE
                     VEEEEAEADEAEETEEESPTEESGSGSNAILPTPEAPKGRDIDLFLKGIRTAETDIPQ
                     TGKAHYTGTWEARIGEPIQWDNHADKAAKAVFTVDFGEKSISGKLTEQNGVEPAFYIE
                     NGKIEGNGFHATAHTRESGINLSGTGSTKPQSFKASNLRVEGGFYGPQAAELGGTIFN
                     NDGKSLGITENIENEVEVEVEADVGKQLEPDEVKHKFGVVFGAKKDMQEVEK"
BASE COUNT         1153 a         1438 c         1244 g         1540 t
ORIGIN      
        1 cgataaattg ccttagcatt gaatgtctag attcccgcct gcgcgggaat gacggcggaa
       61 atcttgttta tatcgaatca aaaaaacctg caccttaatc agttggcggt ttagtccgac
      121 ttttggggtg cagatcaagc tttcagacgg tatttccttt aaaacttcat ttcaagcgcg
      181 aggctgaagt tcctgcccgg tgcggcatac cttccatagt tgctgtcgcc gccgtgccgg
      241 tttgccgtgc tttccgcagt ctggcgcaag gattcccaag taacgtagcg gtagttgccg
      301 atattgtaga tagccgccct caaggtcagc cgttttttca gattcagata ggcggaaacg
      361 tctgccgtcg accaagaaga cgacgctctt tttgtcgaat atcgtttttg atcgcctgcc
      421 agataagcaa gctcgtcagg gtttttccct ttggaatagg tcagcataat gtttgcgccc
      481 catttcccct caggctggtc gtatccgaac cccaaaacat aacgcgacgg ctgtaccgca
      541 tccaaagcat agctgcggag ggacagtccc ggccggttgg ataccgattt cggtttgatg
      601 cggttgtacg ccaatgtggt gtacaaacct tcgggcagtt tgccgtacac gccgttccag
      661 tcgatttttc ccaatatatt aacgccttgg agcgacatat tttgggcatt gtaataatcg
      721 cgtatatcaa tctctgtcaa tcgtcctgat gaatccgaca attgggtttt gtgatcggca
      781 acggcaatca tatcggtata gcggttgcgg aagctgctga tttccaaaaa gccgaaatcg
      841 cctttccact gcaaaccgat ttcgcggttg gctgcctttt ccgatttcag ggcgggacgc
      901 tgccagcctt tcggataatc gtgataaatg tctatcccga aaagctcttg gaatgagggc
      961 gttctgaagc cgctggaggc gcggtaagac aaagaaaaat gccggttcgg tttgaacacg
     1021 atgccgctgt tccacgaacg gtcaacatac cgcccgctgc ggacgagttc ttccgacgtg
     1081 gtgaagtttt tccggtcata cctgccgccc aagctgaaat cgaaatattt gccgattgaa
     1141 aaacggtcgt tcaaagaaat gtggatattg ctgccgttga tttttcttgg cacgcatttg
     1201 cgggaacgca aagtttcgat gtagccgcag accgatcctt caatgacttc gggcttgccc
     1261 aaaagatact tgtctttacc gtcggcggtg tactccgagt attccgaaat ccttgccgca
     1321 ttgtgggaaa gctgttcggg gcgggagatc gcgttggaag catcgtaacc gaagcccaaa
     1381 gtcagatggt gtttcgtcca tttgtttttc agcgatttct caaacgaggc attcaaaaca
     1441 ttgtgctgtt cgcggtagtg gaaacggtcg ctgctgtcgt aggaatacgg cttgtctgcc
     1501 gacgcgcggc aggatttgtc cacagcagga tacacggcgc aattcagctt cagcgtgttg
     1561 ttgtcggttg ccacgccctg tttgtcaaac gacaacaccg ccttatccgc ccagttgtcc
     1621 gagtatttct cattttcata acggtacagc aaacccatac ggcggcggcg gtgatgttcg
     1681 tcaataaatt tggtgcggga atatttcaaa cctatgccct tgactaaatt tgtatcgccc
     1741 ttccactctt ctatattcgg cacaaaatac aagccgtcgc ggaaatcgtc gccgtcgtac
     1801 accccgctct tatttctaaa cttgttcgcc tcgtccgtac cgtaatactg tttttccgtc
     1861 atatcgcgga tatcgtaacg ctgtttggta tcctcaaaca cgccgccgac ataatgcctg
     1921 ccgccgaagc ggtagcccag cttggcaagc cacgaaccgc tgcggtaatc catcggatcg
     1981 ggcaatatcc tgccgccgcc cgtgtacgat tgggcggaca ggttttcgtg gcgcgcctgc
     2041 gcctcctgca ccatcgcttc ttcttcactg tttaaagcct gaccttttga acggcgttct
     2101 tccacccagc ggttgagctg gtttttcaaa tagtttccgt tgcccgccaa ttttgccacg
     2161 ggcttggatt cacgctcgcc ctccgccaga aaatattccg cctttttatt cggattctga
     2221 atatcgtatg tctggcggaa cgcgtccaaa cgatctatgc cgtattccac cccgtccgca
     2281 atatcgccgt gcgggcgcgt ttcccgccct tggcgttcgg ttcggattag cagcccttcc
     2341 caaccgtctt tgctgaaccc cgcgccgagc gacttcataa attggcggtt tttactgccg
     2401 taggcagttt ttgcctgtat tccccaactt ttgccgtctg aaatcaggtc tgccgcttct
     2461 ttggtgcgga aggcgaccgc gccgccgagt gcgccgctgc cgtgatcgga cgaaccggcg
     2521 cctttgtcga tttccaccgt gctgatgttt tcatattcga tttcgttgat tgcaccgctg
     2581 ccgccgcgtc cgccgtatcc gctcaacgat ccctgcacgg taaacgcctg tatttgggca
     2641 acgccgtcaa ccgaaaccgc cacacggttt ttatccacgc cgcgtatcga gtagccgccg
     2701 ctcgcgccgt tgccctgttc gacaaccgcc acgcccggat cgtagcgcgt caggtcgcgg
     2761 ataccgagta cctgttcttt gttcaaagtt tccgacgttt tgacgatttt gcccaaaccg
     2821 gtcgcctctt tcgatcgccg tcccactttg gcggcacgga cggtaacctc tttcagggat
     2881 tgggtctgcg cggcatcagg tgtcgccccc cccgcttggg cggcataagc cggaaaagcg
     2941 gttgcaatgg ccaaggcagt cagggtaagc tgaaaaccgt gtttcttatt catttttcca
     3001 cctcctgcat atctttcttc gcaccgaata ccacgccgaa tttgtgttta acttcatcag
     3061 gttctaactg tttgccaaca tcagcttcaa cttcaacttc aacttcattt tcaatatttt
     3121 cagttatacc aagagatttc ccatcattat tgaaaatagt accgcccaat tccgccgcct
     3181 gcgggccgta aaatcctcct tctacacgaa gattactggc tttgaaactt tgaggtttag
     3241 tcgaaccagt tcccgaaaga ttgatgccgc tctcccgagt gtgtgctgtc gcgtggaaac
     3301 cgttgccctc aatcttgccg ttttcaatat agaaagcagg ttctacgccg ttttgctccg
     3361 tcagctttcc ggaaatcgat ttctcgccga aatcaacggt aaatactgct tttgccgctt
     3421 tatccgcatg attgtcccat tgaatgggtt cgccgatacg cgcttcccaa gtgccggtat
     3481 agtgtgcttt tccggtttgc ggaatatccg tttctgccgt gcggatacct ttcaggaaaa
     3541 ggtcgatgtc cctgccttta ggggcttccg gagtgggcag gatggcgttt gaaccgctgc
     3601 cgctttcttc tgtcggcgat tcttcttcgg tttcttcggc ttcatcagct tcagcttctt
     3661 cctcttcaac ttcatcagct tcttcttcga tttcttctgc atcttcttcg gaaacttcgt
     3721 cttcatcgtc gatttcgtct tcgccttctt cgacgctatc aacgcctgta tcctcttcgt
     3781 ccccctcttc atcctgcgcc ttcggttgga cggcggggcg ttcggttttt atccgtccga
     3841 gtttcacata ggtcagaaaa tcgcaacaag cacggacggt cattttcctg ccgtcggcaa
     3901 gctcgatgat tttctcttgg ctaaccaaag gaatttcacg cccttcgaca agaagtttgt
     3961 cgggatgacc aaaatcgggc atagtggaaa tggcaaactt acgggcatgg tcatcagtcg
     4021 cctcgtcaac ggaaattttt agagaatcca agattttggt gtgttttcca gacggcaggg
     4081 caggttttgt atctgctgcg tttgctgcct ctgttttttg tttgcctgcg aatacgccga
     4141 atacgctgtt gtcattgctg ataaaccgtc cggcaagctc ttctccgtta tcgccgaaaa
     4201 aaccgccctc aagccgctga tcggcatcgg tatggaaaaa caaatattct ttatcagcgt
     4261 gattcgtctt cacctctgtg ttaactttgg cactgccggt aaagcggttg ccgtccaatt
     4321 ttgcagtaat gtcgtaaatg gtcagcggtt tcttttcatc ggttttcctt tgcacatact
     4381 gatttttaat cagcttgcca ttcagagttt tttggtcgaa atcaaccgta tattcggcag
     4441 gatgttttcc ctccccctcc cggtcgtcgg cagtcgcagc ataagaagtt gctccgacat
     4501 tttgaccgta ataaacgata taacccaaat cttcgctagg atctcctgct tttcctgttc
     4561 gatgacgtat ggcatcggtc atatattgcc agttgccgga atattttacc gttcccgcgc
     4621 tcggtaaaga ttgcgaagga tgttctccag aataatatac aaaaccgtca taaccaaacc
     4681 ggttggtaga ctgcttgtaa tttgaagtcc actcaatttc atcttttccg tctttagtat
     4741 atacataacc tgcatccaca aatttataac catatttttt attttcattt tttgatgtcc
     4801 taatttgtac attaggattg agttgatgaa ttttatcttc aagctgctga cgttgatctt
     4861 tttttgaacc gtataaaaac agaacatcac cttctttaaa cgacagcttt tcttctgctt
     4921 gtttgctatt tggaaaatct ttgccatcct tatcagaagt tgcgatattc cgcctcaaca
     4981 gccgcattgc cgcaccaacg gcgggttgat tgaccggcgt ggtttctacc gaaggcccgg
     5041 cagggggcgg agtgggaacg tccttagact tgaaagtgac ggggtacgcg gtcggcgttg
     5101 attcgacaac aggctgcacg ccgaaattgc cgccgataca agatgccaaa agtaagggca
     5161 acaagacaat gccgccataa ttcggtttac acatccctac ttttcctcct atttgattaa
     5221 taataattat cattatatta atatgtacag ataatatcaa cccgttttta tagtgaatta
     5281 acaaaaatca ggacaaggcg acgagccgca gacagtacag atacattccg tcattcccac
     5341 gaacctacat cccgtcattc ccacgaacct gcacc
//
LOCUS       DAXHTY010000108         5244 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXHTY010000108 DAXHTY010000000
VERSION     DAXHTY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.108"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            96..1571
                     /gene="trpE"
                     /locus_tag="ACFNMK_001789"
     CDS             96..1571
                     /gene="trpE"
                     /locus_tag="ACFNMK_001789"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC6364999.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGTPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTAKADSLGTPDILLMLSQEL
                     AVVDNLSGKIYLIVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSSLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERNDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKEGVTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSNPESEWQETQNKARAVIHAAQMVQEGLDK"
     gene            1568..2113
                     /locus_tag="ACFNMK_001790"
     CDS             1568..2113
                     /locus_tag="ACFNMK_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFC6365000.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKNREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            2110..3246
                     /locus_tag="ACFNMK_001791"
     CDS             2110..3246
                     /locus_tag="ACFNMK_001791"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC6365001.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPGSDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHGGVDCVLEKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIIPARLSADVQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTIDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGKPDWFPLQS
                     RPDARLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            3293..3775
                     /locus_tag="ACFNMK_001792"
     CDS             3293..3775
                     /locus_tag="ACFNMK_001792"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6365002.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            3881..4936
                     /locus_tag="ACFNMK_001793"
     CDS             3881..4936
                     /locus_tag="ACFNMK_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6365003.1"
                     /translation="MKTYAPVLYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
BASE COUNT         1496 a         1595 c         1115 g         1038 t
ORIGIN      
        1 aagggcctct cggtcagact gtcaacaatt ccgataaaat gctatactcg cgcccgttgc
       61 cgcgccgtcc gaaaccgccc tttgggaaag cagaaatgat cagcaaacaa gaataccaag
      121 cccaagccgc ccaaggctac aaccgcatcc cgctcgtgca agaactcctt gccgatttgg
      181 atacgccgct ttccctctat ctcaaactcg ccaaccgccc ctatacctac ctgctcgaat
      241 ccgttgtcgg tggcgaacgt ttcggccgct attcttttat cggcctgcct tgcagccact
      301 atctcaaagc cagcggcaaa cacgtcgatg tttatcaaaa cggcgaaatt gtcgagcaac
      361 acgacggcac ccccctgccc tttatcgaag ccttccacaa ccgcttcaaa acacccgaaa
      421 tcccaagcct gccgcgcttt accggcggac tggtcggcta cttcggttac gaaaccatct
      481 acaatttcga acacttcgct caccgcctga aaaacaccgc caaagccgac tcgctcggca
      541 cgcccgacat cctcctgatg ctctcgcaag agcttgcagt agtggataac ctaagcggta
      601 aaatctacct gattgtttac gccgatccgt cgcagcccga cggctacgaa cgcgcacgcg
      661 aacgcctcga agacatccgt acacagttac gccaaagttg cgccatcccg ctttcgctcg
      721 gcagcaaaca caccgaagcc gtcagcgaat tcggcgaaga gccgttcaaa gcctgcgtta
      781 acaaaatcaa agactacatc tttgcaggcg actgcatgca ggttgtcccc agccagcgca
      841 tgagcatgga attcaccgac agctcgctcg ccctctaccg cgccttgcgc acgctcaacc
      901 cttcgcctta tctcttctac tacgatttcg gcgatttcca catcgtcggc tcctcgcccg
      961 aaatcctcgt ccgacgcgaa cgcaacgacg ttatcgtccg ccccatcgcc ggtacgcgcc
     1021 tgcgcggcaa aacccctgcc gaagaccttg ccaacgaaca agatttgtta agcgacgcca
     1081 aagaaatcgc cgaacacgtc atgctcatcg atttaggacg caacgacgtc ggccgcatca
     1141 gtaaaaccgg cgaagtcaaa gttaccgaca aaatggtgat tgaaaaatac tcccatgtga
     1201 tgcacatcgt ttccaacgtc gaaggccgtc tgaaagaggg cgttaccaat atggacatcc
     1261 tcgccgccac gttccctgcc ggcacacttt ccggcgcacc caaagtccgc gccatggaaa
     1321 tcatcgaaga agtcgaacca agcaaacgcg gcatctacgg cggcgccgtc ggcgtatggg
     1381 gtttcaacaa cgacatggat ttggcaatcg ccatccgcac cgccgtagta aaaaacaaca
     1441 cgctatacgt ccaaagcggc gcaggcgtag ttgccgattc caatcctgaa tcagaatggc
     1501 aggaaacgca gaacaaagcc cgtgccgtca tacacgccgc gcaaatggta caggaagggt
     1561 tggataaatg acttatgaaa aactttatga gcttcgccaa acactcagac cgactaccaa
     1621 agacggtctc tatacagata atgaaaaaaa tcgagaaatc ctaacggtta gatggagcgg
     1681 caacacagaa aatcctaaaa atttctcagc tgtcgctata ggaattaatc caagtaaagc
     1741 aaatgatgaa agaagcgaca aaacccttac ccaacttgca cgtttcttgg atatgtacgg
     1801 ttttaccaac ttcaaaatgt taaatatttt ttccagttac tctacacagc aaacaggaat
     1861 tagggcaaac acccaaaccg atttttccaa atttaaagga tgtttggaag atgctgatat
     1921 gattattctt gcatggggta cagaccgcgg tgcttataag gatgaaaaaa acagaatact
     1981 ggaatttcta aaagcagaaa aatttatgga aaaagtattc tgtatttctg aaactggtaa
     2041 ttccagcgat acaagacatc cgtcaagaat tagttatagc tatcaacttg tacaatttga
     2101 agagtcagca tgaaaaacat atctctttct ccgcccgcca tgcttggcat cctcggcggc
     2161 ggacaattag gcagaatgtt taccgttgcc gccaaaacca tgggctacaa agtaaccgtt
     2221 ctcgaccccg atccggacgc gccggcagca gaatttgccg actgtcattt atgcgcgccg
     2281 tttaacgacc aagctgcttt ggacgaattg gcaaaatgcg cggcggtgac cactgaattt
     2341 gaaaacgtca atgccgatgc gatgcgtttt ctcgccaaac acaccaatgt ttcccccggc
     2401 agcgactgcg ttgccatcgc gcaaaaccgc attcaggaaa aggcatggat acgcaaagca
     2461 ggattgcaaa ccgcgccgta tcaagtggtt tgtaaggctg aagacattac tgaagcaagt
     2521 gcgcaattct tgcccggcat cctgaaaacg gccactttgg gctacgacgg caaaggtcaa
     2581 atccgcgtca aaacggtgga tgaactcaaa gctgcgtttg ccgaacacgg cggcgtggat
     2641 tgcgttttgg aaaaaatggt ggatttgcgc agtgaaattt ccgtaatcgt atgccgtttg
     2701 aacaatgaca acgtgcaaac tttcgaccct gccgaaaaca tccacgaaaa cggcatcttg
     2761 gcttattcca tcatccccgc gcgactgagt gccgacgtgc agcaacaggc gcggcagatg
     2821 gcgcaacgct tggcggacga attggattat gtcggcgtat tggcggtaga aatgtttgtt
     2881 gtcggtgaca cacatgaatt ggtcgtcaac gaaatcgcgc cgcgtccgca caactccggc
     2941 caccatacca tcgatgcctg cgcggcagac caattccagc aacaggtccg cctgatgtgc
     3001 aacctgccac ctgccgatac caagttgctt tcttcatgct gtatggcgaa tattttgggc
     3061 gacgtttggc aggaagacgg cggcaaaccg gattggtttc ccctgcaaag ccggccggac
     3121 gcgcgcctgc acctttacgg caaaaaaacc gcgcacaaag ggcggaaaat gggacacttt
     3181 accgttttaa ccaccgattc ggacaccgca tttcaagaag caaaaaaact gcatcagtcc
     3241 ctataatccg accaacggca cagatgccgt ctgaagccgc cgacatactg ccatgaacct
     3301 gctgacactg ttacgccccg ccaccgtgca agactgcaaa gacatcttca aagtccacct
     3361 gcactccgtc caatacacct gcatcctcag ctacaacgaa cacgccctaa aagtatggga
     3421 aggcttgcta aacacagaaa gctacctgcc gaccatttcc gatcccgaca aagcactttg
     3481 ggttgccgaa tacaaaggca acatacaggg atttttccaa atcgactgcc aagaagcaca
     3541 actcgatgcg ctttacgtcc accccctgtt ccacaacctc ggactgggca ctgccctgct
     3601 tcatcaggcg gaaaccatcg cccacaaatc cggtttgagc tttctgaaac tctacgcatc
     3661 gctcaactcc gtcccgtttt accttctcaa ccgctacgaa tcgctcggtt cggcagtatt
     3721 gcagcttgac cccagcatca aaatcaaatg cgaactgatg cggaaacacc tctgatccgt
     3781 ccttataacc aaaactgata atcttcaaat caaacattat ttccacaagc gcagacagcc
     3841 gtataaagtt gccccatcta ccaaccgaca ggacaagcat atgaaaacct acgcaccggt
     3901 actctatacc gcagccctgc tcaccgcctg cagccccgca gccgattcaa atcatccgtc
     3961 cggacaaaat gccccggcca ataccgaatc cgacggaaaa aacattaccc tgctcaacgc
     4021 ctcatacgat gtggcacggg atttttacaa agaatacaac cccttattta tcaaaacata
     4081 ccaatccgaa caccccggca catccgtcag catccaacag tcccacggcg gctccagcaa
     4141 acaggcatta tccgtagcca acggccttca agccgatgtc gtaaccatga accaatcctc
     4201 cgacatcgac ctgctcgaaa aaaaaggact ggtagaaaaa ggctggcaac aagccctccc
     4261 cgaccacgcc gcgccctaca ccagcactat ggttttcctt gtccgaaaaa acaaccccaa
     4321 acagatccgc gattggaacg accttgccaa agacggcgtt aacatcgtca tcgccaatcc
     4381 caaaacctcg ggcaacggac gctacgcctt cctcggcgca tacggttacg gtctgaaaac
     4441 caccaacggc aacgaacagg aagcccaaaa actcgtcgca tccatcctca aaaacacccc
     4501 cgtttttgaa aacggcggac gcgccgccac caccaccttc acacaacgca acatcggcga
     4561 cgtactcatc acttttgaaa acgaagccaa ctacgtcagc aaaaaactga cccaaggaca
     4621 gtttgaaatc gtctatccga gctacaccat ttccgccgaa agccccgtcg ccgtcgtcaa
     4681 cagcgtcgtc gcgaaaaaag gcacgcaaaa aaccgcccgc gcctatctcg aatacctttg
     4741 gagcgaaccg gcgcaagaac tcgccgcctc actctacctg cgcccccgca accccgaagt
     4801 attggcaaga cacaaagccg acttcccaga cttagacacc ttctctcccg aagaaaaatt
     4861 cggcggatgg gacaacatca tgaaaaccta cttcgccgac ggcggcatat tcgaccgcct
     4921 gaccgcacaa aaatgaccgc tataccgtct gaaccgcctt tcagacggca tttcacacat
     4981 caaaacagaa aaaccgcgcc caaaatcatc atcacaggac acaacggcaa accggataca
     5041 aaacccaaca ccgccgccaa aatgccgtct gaagccgtcg gcgcaaccat ccttatctgg
     5101 ggcccgcttc aggggcgcat aactttcctt aaccatcctt ttgacagcca aataaacagt
     5161 gcttttgcct gtcagaaaca aaaccaatat tttgcggttc gtatcggaac atttatagtg
     5221 gattaacaaa aaccagtaca gcgt
//
LOCUS       DAXHTY010000109         4535 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXHTY010000109 DAXHTY010000000
VERSION     DAXHTY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.109"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..192
                     /locus_tag="ACFNMK_001794"
     CDS             <1..192
                     /locus_tag="ACFNMK_001794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365004.1"
                     /translation="RRKPVFGFRLSGGKGILQRSHPVIFTKTENQKQQPEIRHSRAGG
                     NPDPQRGRNLSEITETGRT"
     gene            513..1330
                     /gene="opc"
                     /locus_tag="ACFNMK_001795"
                     /gene_synonym="opcA"
                     /pseudo
     CDS             513..1330
                     /gene="opc"
                     /locus_tag="ACFNMK_001795"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
     gene            1493..2137
                     /locus_tag="ACFNMK_001796"
     CDS             1493..2137
                     /locus_tag="ACFNMK_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6365005.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            2156..4066
                     /locus_tag="ACFNMK_001797"
     CDS             2156..4066
                     /locus_tag="ACFNMK_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6365006.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHDLEHNSDDVLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSL
                     VFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            4087..4452
                     /locus_tag="ACFNMK_001798"
     CDS             4087..4452
                     /locus_tag="ACFNMK_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365007.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
BASE COUNT         1244 a         1156 c         1036 g         1099 t
ORIGIN      
        1 agacgaaaac ctgtgtttgg gtttcggctg tcgggaggga aaggaatttt gcaaagatct
       61 catcctgtta ttttcacaaa aacagaaaac caaaaacagc aacctgaaat tcgtcattcc
      121 cgcgcaggcg ggaatccaga cccccaacgc ggcaggaatc tatcggaaat aaccgaaacc
      181 ggacgaacct agattcccgc tttcgcggga atgacggcag agtggtttca gttgctcccg
      241 ataaatgccg ccatctcaag tctcgtcatt cccttaaaac agaaaacaga aaaccgaaat
      301 cagaaaccta aaatttcgtc attcccataa aaaacagaaa accaagtgag aataacaatt
      361 cgttgtaaac aaataactat ttgttaattt ttattaatat atgtaaaatc cccccccccc
      421 accgaaagct taagaatata attgtaagcg taacgattat ttacgttatg ttaccatatc
      481 cgactacaat ccaaattttg gagattttaa ctatgaaaaa aacagttttt acatgtgcca
      541 tgattgccct gaccggtact gccgccgctg cacaagagct tcaaaccgct aatgagttta
      601 ccgtccacac cgacctctct tccatttctt caactcgtgc tttcctgaaa gaaaaacaca
      661 aagctgccaa acacatcggc gtacgtgctg atattccttt tgatgccagc caaggcatcc
      721 gcttggaagc cggtttcggg cgcagcaaaa aaaatattat taatttggaa acagatgaga
      781 acaagctggg taagactaaa aatgtaaaac tgcccaccgg cgttcctgaa aaccgtatcg
      841 atctttacac aggctacacc tatacccaaa cgttaagtga ttctttaaat ttccgtgtgg
      901 gtgccggctt gggttttgaa tcttcaaaag acagcattaa aaccaccaag catacgcttc
      961 acagcagccg tcagtcgtgg tcagccaaag ttcacgcgga tttgctttcc caactgggta
     1021 acggctggta tatcaaccct tggtctgaag tgaaatttga cctcaagtcc cgctataaat
     1081 taaacaccgg cgttaccagt ctcaaaaaag acatcaatca aaaaaccaac ggctggggct
     1141 ttggattggg tgcaaatatt ggtaaaaaac tgggcgaatc cgccagcatc gaggcgggcc
     1201 gttctacaaa caacgcactt acaaagaatc cggcgagttt agtgtaacaa ccacgagtgg
     1261 cgacgtatcg ctcaccatcc cgaaaaccag tattcgtgaa tacggcttgc gcgtcggcat
     1321 aaaattctga tgatttgaaa tcatcattgt cgctttaaat gccaaaccgc aaactatttt
     1381 ggtttgcggt ttttacgtga aatgaatttg aatagccgat gccgtctgaa aatttcagac
     1441 ggcatttttc caatcggtta aaatacgcgg tttgtttttt taggaacgcg ccgtgcttgc
     1501 ctccgccatc gacttcatcc tccatatcga ccaacacctg ctcgcgctgt cggcgcaata
     1561 cggtgtgtgg atttatgcga ttctgttttt gattgttttt tgcgaaaccg gcctgattgt
     1621 tacgccctta ctgcccggcg attcgctgct gtttgccgcc ggcgggattg ccgcgctggg
     1681 cggtatggat attcatttga tggttgcatt attaagcctt gccgccatac tcggcgacgc
     1741 gctcaatttt accgtcggca aatatttcgg cggcaggctg ttcgccaacc ccgattctaa
     1801 aatcttccga cgtgaatatc tcgataaaac ccaccgtttt tacgaaaaac acggcggcaa
     1861 aaccatcatc attgcccgtt ttatgcccat cgtccgcacc ttcgccccct tcgtcgccgg
     1921 tatggggaaa atgcactatg ccaaatttat ccgctacaac atcatcggtg gcttgttgtg
     1981 ggttatcctg ttttcttacg cgggctattt ctttgccaac ttccccgtcg taaaaaacaa
     2041 tctgggcttg gtgatgggcg gcatcattat tgtttccgtc ctgccgggtg cggttgaaat
     2101 tgcccgcgcc aaacttgcgg taaaatccaa acggtaactg ttgagaattt gtatcatgaa
     2161 tatattatct gtagaaaacg cttcttttgc catcggccac gttgccttgc tcgacaaaac
     2221 ttcttttcaa ctcgacagcg gcgaaaaagt cggcttaatc ggacgcaacg gcgcaggcaa
     2281 gtcttcgttt ttaaaaatcc tcgccggttt gcaaaagctc gacgacgggc agattattgt
     2341 tcaaaacaac ctcaaaatcg tttatgtacc gcaggaatcc ttttttgata aggacgcaac
     2401 cgtatttgat accgttgccg aaggtttggg cgaaattcgc gatttattgc gccgttatca
     2461 tcatgtcagc catgatttgg agcacaattc agacgacgtt ttattaaaag aactcaacga
     2521 attacaactc gaaatcgaag cgaaggacgg ctggaagctg gatgcggcag tcaagcagac
     2581 tttgggcgaa ctcggtttgc cggaaaacga aaaaatcggc aacctttccg gcggtcagaa
     2641 aaagcgtgtc gccttggcgc aggcttgggt gcagaagccc gacgtattgc tgctggacga
     2701 accgaccaac catttggata ttgacgcgat tatctggttg gaaaacctgc tcaaggcgtt
     2761 tgaaggcagc ttggtcgtga ttacccacga ccgccgtttt ttggataata tcgccacgcg
     2821 gattgtcgaa cttgaccgcg gcattctacg ttcctatccc ggctcgttct ctaaatacag
     2881 tgagaaaaaa gcgcaagagt tggcagtcga ggcggaacat aaccgcttgt ttgacaaatt
     2941 tcatgctcag gaagaagcgt ggatacgcaa aggcattgaa gcgcgccgta cccgtaacga
     3001 aggccgcgtg cgccgcttgg aagagctgcg ccgccaacgt gcagaacgcc gcaacgtgca
     3061 gggacaggtc aactttaagc tcgacagcgg tgagaagagc ggcaaaatca tcgccgaatt
     3121 ggagcacgct tcgtttgcct acggcggcaa agtcattatg gacaaattct ccgccatctt
     3181 gcagcgcggc gacaaaatcg gcttaatcgg tccaaacggc atcggcaaaa ccacattttt
     3241 aaaactgatt ttgggcgaat tgcagccgac ctacggcaga atccgtatcg gcagcaagca
     3301 ggaagtcgcc tatttcgacc agttccgcag cgcgttgaat gaaaacgaca ccgtgtttta
     3361 cacgctcgga cagggcaatg attacgttga agtcggcggt aagaaaaagc atgtgatgag
     3421 ctatttggaa gatttcctgt tcccccctgc ccgcgcgcaa agtcctgttt cttcactctc
     3481 cggcggcgaa cgcaaccgcc ttttgctggc aaaactcttt acccgtcccg ccaatatttt
     3541 ggtcttggac gaaccgacca atgacttgga catcgacacc caagagctgc tcgaagacct
     3601 gttgcgcgat tatcaaggca cggtattcct tgtctcgcac gaccgtatgt tcctcgataa
     3661 cgtgattacc caaagccttg tttttgaagg aaatggaagg ttaaaagaat atatcggcgg
     3721 ctatcaggac tacatcgacg caaaatcacg cgaagcgaag attcagacgg aatccgcacc
     3781 caaaacagcc gatgccgaac cggtgaaaga aaaacccaaa gccaaccgca cggtcaaact
     3841 gtcctacaaa gaacagcgag aactcgatgc cctgcccgac gaaatcgccg ccttggaagc
     3901 cgaacaggca gaaatcaacg cgcagctttc cgatcccgaa atcttcaaag attacgaaaa
     3961 agccggcgca ctgcaaagcc gcgccgaaga aatcgaaatg ctgcttttgg aaaagctgga
     4021 acgctgggag ctgctggaaa ccaaacagaa cggcaatgcc gtctgaaatc aaacaggaag
     4081 aaaaaaatgt tcaaacgacc tgaagaaatc atcgtcctga tacttgccgt cctctggatt
     4141 gccggtacgt attttctcgc cgccctgttt ggagcggacg cttataccgt gttaaaaatc
     4201 actgccctta ccctgctttg gtctgccgca tcattcctcc tttggcaaaa aaacctcagc
     4261 cggcatatct ggccgctgct gctcggctgc ctgactgcct gttggtggcc gtatctgaat
     4321 caatcggtcg cacccggttc tttaccttgg tatgcatcat ggacgttcaa aaccatctta
     4381 gccctgattc ccgtaaccgc aggttatctg tttaaatgga aacaataccg gcagcgcaaa
     4441 acaaagccgt aagcccgtct ttttcagacg gcggcggatt cgcatttgaa gtgcaacttt
     4501 ccctaacaga aaaaggccag tatgcggtag catac
//
LOCUS       DAXHTY010000110         4485 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXHTY010000110 DAXHTY010000000
VERSION     DAXHTY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.110"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(1..632)
                     /locus_tag="ACFNMK_001799"
                     /pseudo
     CDS             complement(1..632)
                     /locus_tag="ACFNMK_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694379.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(625..816)
                     /locus_tag="ACFNMK_001800"
     CDS             complement(625..816)
                     /locus_tag="ACFNMK_001800"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6365008.1"
                     /translation="MRKVFHYEQNRALTVRELAALQSFPDNFIFCGSKIAQQQVGNAV
                     PPLLAKAIAESILKMSENE"
     gene            complement(794..1624)
                     /locus_tag="ACFNMK_001801"
     CDS             complement(794..1624)
                     /locus_tag="ACFNMK_001801"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6365009.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKTSRLYYRRYAQSFSL
                     "
     gene            complement(1655..3928)
                     /locus_tag="ACFNMK_001802"
     CDS             complement(1655..3928)
                     /locus_tag="ACFNMK_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC6365010.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPEMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGITAAELISNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(3992..>4485)
                     /locus_tag="ACFNMK_001803"
     CDS             complement(3992..>4485)
                     /locus_tag="ACFNMK_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6365011.1"
                     /translation="VEPPTSGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNV
                     DIDYVKAFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDS
                     RFADKCKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYN
                     SLP"
BASE COUNT         1016 a         1140 c         1138 g         1191 t
ORIGIN      
        1 ggcaaaaggt tgttgggaat accgttgggc tgttcgccga tcaagccgct ttcgtgcacc
       61 ccaatcgggt tgaaatagcg caacaaaatc acgctccaac gcggatcggc cttttggatg
      121 tcggttaaca tccgttccac catcgctttg gatgcgccat aaggattagc ggtatcgccc
      181 gggcgcatat cttccgtata gggcatttta cgtatcatgg cgcgacgcat taaagtaaac
      241 gccaaagcgc gtttatactg gttatccagt ttccctatat ttttacgtta caaatcaagt
      301 tgttggtatt catcgtgaaa atagaatttt tcggcataac gctgactcat aaaaccggca
      361 tgcgggctgc ctgaaaaaat cattgcgaca tcatcgtcat ttaatgtttc atggttgttt
      421 gctattaatg ctaaagcaat ttggtaatta attgccaaaa tatcgttagt aatcacacga
      481 tagccgcgtt ttttggcttc gtaggcaaag gaacagcctc cactaaatac atctgcaact
      541 gtatctacat cagacggaag ctggtcacaa atccaggaag ctattttctc tttattaccg
      601 atatagttaa ttttcggata ttgcttattc attttcactc atttttaaaa tactttcagc
      661 aatagctttt gccaataaag gcggtacggc gttaccaacc tgctgctggg caattttgct
      721 gccgcaaaaa ataaaattat cagggaaaga ttgtaaggca gctaattcac gaacggttaa
      781 cgcccgattc tgttcatagt gaaaaacttt gcgcatatct cctgtaatac aaacggctgg
      841 ttttgttgct gttgtaacgg atgtatttac ggatatcacc tgttttcgga cgtaatggtt
      901 caggaatatc gttacggtta cctccatttt taacaaatgc cattttttct aacatttgtg
      961 ccgaatgatt catagcttca tgatttgcaa cgtgtggatt gctttcgcca gcagccagtt
     1021 ttggaaaatg tcctattgct gatccaacag tctgatggga aatctgcaaa ggttcgggaa
     1081 aggaaatttt gcctttatcc ctcctcccga taaatatcac tcggctacgt atctgaggaa
     1141 caccgaaatc ggctgcactc agtatcttac attccaccga ataaccgata ttctgaaatg
     1201 cttgaataat ctcaatacgt gttttacctg aattgtgtgt atagagtcgc gctacatttt
     1261 ccataacaaa aaaatatggt tggacaattt taactattcg gacaaactct ttaaataaat
     1321 ggttgcgtgg gtcatctgta aatgtccgtc caatctttcc tgccatacta aaaccttgac
     1381 aaggtggtcc tccaataatc aaatcaactg cttgtccgtt aagacaattg attaaatctt
     1441 gttcggttag tgtggttaaa tctttttgca gtaattgatg atgggggaag ttggtacggt
     1501 aagtctgaca ataatcagac tccatttcaa cagaaagcaa ttgttggaat ccggcttgtt
     1561 caaaacccaa ggataggcct cctgctcctg agaaaaggtc aatataggta agtggtgtgt
     1621 gcataagtca aaatccataa actcttcgat tattttaccg cttcagcttc gcaaacgcat
     1681 ccgccatcgc cgaattggct ggggcgcggt cgttgcgttg gggtttgcgg ccggctgtcc
     1741 gttcgcggct gcggttttca gacggcattt tatgttttgc gccgcccggt tcgtcatcca
     1801 agcgcatggt cagcgcgatg cgtttgcgtg cggcatcgac ttccagcact ttcactttca
     1861 ccacgtcgcc ggctttcacg acttcgcgcg ggtcttggac gaatttattg gatagggcgg
     1921 agatgtgcac caagccgtcc tgatggacgc cgatgtccac gaacgcgccg aagttggcga
     1981 cgttggaaac cacgccttcg agtatcatac cgacttgcaa gtcgctgatt tcgtggatgc
     2041 cttcggcaaa cgatgccgtc tgaaacgcgc cgcgcgggtc tcggccgggt ttttccagct
     2101 cggacaggat gtccagaatg gtcggcaggc cgaagcgttc gtcggtgaag tcggacgctt
     2161 tgatttgctt cacgcgctcg cggttgctga tgagttcggc ggcggtaatg ccttgttgcg
     2221 ccagcatttt ggcaacgacg ggataggctt cgggatgcac cgcgctcgcg tccagcggct
     2281 ctttgccgcc gttaatccgc aaaaagcctg ccgcctgctc gaaggttttt tcgcccaaac
     2341 gcggtacttt cagcaatttt ttgcggctgt cgaacgcgcc gttttcatcg cggtaggcaa
     2401 cgatgttttg ggcaagggtt tgattcaaac cggaaattcg cgccaagagc ggggcggagg
     2461 cggtgttcac gtccacgccg acggcgttca cgcagtcttc gaccaccgcg tccagcgatt
     2521 tggcgagctg gctttggttc acgtcgtgct gatactgtcc cacgccgatg gatttggggt
     2581 cgattttgac caactcggca agcgggtctt gtagcctgcg ggcgatggac accgcgccgc
     2641 gcagggaaac gtccaagtcg gggaactcgc gcgccgccag ttcggacgcg gaataaatcg
     2701 atgcgccggc ttcggaaacg acgattttgt gcagccccat ttccggcatt ccgcgcacca
     2761 gttcgcccgc gattttgtcg gtttcgcggc tggcggtgcc gttaccgatg gcgatgagct
     2821 tcacgccgtg ctgtttaatc aggcgcgaca gcgttgccaa catattgttt tcttgatgca
     2881 aatagacgat gacggtatcc agcagcttgc cggtgtcgtc caccacggcg catttcacgc
     2941 cgttgcggta gccggggtcg agacccagcg tggtcagccg tccggcgggc gcagcaagca
     3001 gcaagtcttt gagattgcgg gcgaacacgg taatcgcgtc ggtgtcggcg gcttctttca
     3061 aacgattgag agcttcaagt tccaacgaca aaaagatttt cgcgcgccaa gtcaggcgca
     3121 cggtgtcgcg cagccatttg tggccgtctg aaactttgaa gcggcgggcg atgatttgct
     3181 cgtattcgct ttgctgcgta atcggcgtgt cgtcgggctg atatttgagc gcgatgttca
     3241 acacgccttc gttgcgtccg cgcaaaaccg ccagcgcacg atggctgggc atcgcacgaa
     3301 taggttcgcg gtggtcaaaa taatcgctga atttttcgcc ttcggtttct ttgcctccaa
     3361 caacttgcgc gtggatttcg gcttcgttcc acagcttgtc gcgcagcgtg ccgatgagtt
     3421 ccgcgtcttc ggcaaactgc tccatcagaa tcgcacgcgc gccgtccaac gcggctttgg
     3481 catcggggac gttttcgttc aggtagcctt gtgcggcggc ttccacgtcc tgcgactgct
     3541 cggcaagcaa cacgtccgcc agcggctgca aaccgtgttc gcgcgcgatt tgcgctttgg
     3601 tgcggcgttt gggtttgtag ggcagataca ggtcttccag cgcggttttg ttatcggcgg
     3661 cttcgatttg cgccctgagg tcgtctgaaa gcttgccttg ctcttcaatg ctttttaaaa
     3721 caacggcttt gcgctcttcc aactcgcgca gatattgcag ccgctcggca agccggcgca
     3781 actgcgtatc gtccaacccg cccgtcgctt ccttgcggta gcgggcgata aacggcacgg
     3841 tcgcgccgtc gtccaaaagc tcgacggcgg cggtgatttg cgcggcagtc gcggagagtt
     3901 cttgggaaag aatttgagta atgttcatca atagaattcc aacggacagg ccgtctgaaa
     3961 tttcagacgg cctgatttaa aaacaatcgc tttaaggcag cgaattataa tattcgtagg
     4021 ctttgtccat atcttcaaac tggtacatat gccctttttc cagcaccatc gcattatcgc
     4081 aatattgctt catggcgctg tggctgtgcg acaccaagat gatggaacgg tctttgcgct
     4141 tttcaaacaa ctcgtactta catttatcgg caaaacgcga gtcaccaact gcaatcactt
     4201 cgtcaatcag gtaacagtca aactccaccg ccaacgacag cgcaaaagcc aaacgcgctt
     4261 tcatacctga agaatagcgt ttcaccggct catacaaata ttgccccagc tccgaaaatt
     4321 cttccgtaaa cgctttcaca taatcgatat cgacattata aatccggcag ataaaacgca
     4381 gattatccat cccggtcagg cttccttgga aggcaccaga aaacgccagc ggccaagaaa
     4441 tactcatggt tcgcttgatt tcgcccgagg taggcggctc gacac
//
LOCUS       DAXHTY010000111         4355 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXHTY010000111 DAXHTY010000000
VERSION     DAXHTY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.111"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            123..1970
                     /gene="ltgA"
                     /locus_tag="ACFNMK_001804"
     CDS             123..1970
                     /gene="ltgA"
                     /locus_tag="ACFNMK_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC6365012.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETADLSAS
                     VPTRPAEPEGKTLADYGGYPSALDAVKQKNDAVVAAYLENAGDSAMAENVRNEWLKSL
                     GARRQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAAMGNTQEAEKLY
                     KQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAVKRALVLFQNSRT
                     AGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREIA
                     GKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTPL
                     EGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGIVPAR"
     gene            2259..2471
                     /gene="rpsU"
                     /locus_tag="ACFNMK_001805"
     CDS             2259..2471
                     /gene="rpsU"
                     /locus_tag="ACFNMK_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC6365013.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2603..3049
                     /locus_tag="ACFNMK_001806"
     CDS             2603..3049
                     /locus_tag="ACFNMK_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC6365014.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(3097..3243)
                     /locus_tag="ACFNMK_001807"
     CDS             complement(3097..3243)
                     /locus_tag="ACFNMK_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365015.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNPFRCIETVLPLPIRYWDI
                     ENAV"
     gene            complement(3248..3640)
                     /locus_tag="ACFNMK_001808"
     CDS             complement(3248..3640)
                     /locus_tag="ACFNMK_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC6365016.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFSGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3712..4317)
                     /locus_tag="ACFNMK_001809"
     CDS             complement(3712..4317)
                     /locus_tag="ACFNMK_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC6365017.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
BASE COUNT         1048 a         1229 c         1214 g          864 t
ORIGIN      
        1 atgtcggaaa cattccaaag gcggtgcagt ttcggcatat aattcgggca aacgcctgtt
       61 cagacggcat tttgtctttt ccaaccctga ccgttcaggg ttccgattct taaggaaatc
      121 cgatgtacct accctctatg aagcattccc tgccgctgct ggcggccctg gtgcttgccg
      181 cgtgttcttc gacacacgcg cccagcagca agaccctggc agacaatata gaaactgccg
      241 atctttcggc aagcgttccc acccgccctg ccgaacccga aggaaaaacg ctggcagatt
      301 acggcggcta cccgtccgca ctggatgcag tgaaacagaa aaacgatgcc gtcgtcgccg
      361 cctatttgga aaacgccggc gacagtgcga tggcggaaaa tgtccgcaac gagtggctga
      421 agtctttggg cgcacgcaga cagtggacgc tgtttgcaca ggaatacgcc aaactcgaac
      481 cggcagggcg cgcccaagaa gtcgaatgct acgccgattc gagccgcaac gactatacgc
      541 gtgccgccga actggttaaa aatacgggca aactgccttc gggctgcacc aaactgttgg
      601 aacaggcagc cgcatccggc ttgttggacg gcaacgacgc ctggaggcgc gtgcgcggac
      661 tgctggccgg ccgccaaacc acagacgcac gcaaccttgc cgccgcattg ggcagcccgt
      721 ttgacggcgg tacacaaggt tcgcgcgaat acgccctgtt gaacgtcatc ggcaaagaag
      781 cacgcaaatc gccgaatgcc gccgccctgc tgtccgaaat ggaaagcggt ttaagcctcg
      841 aacaacgcag tttcgcgtgg ggcgtattgg ggcattatca gtcgcaaaac ctcaatgtgc
      901 ctgccgcctt ggactattac ggcaaagttg ccgaccgccg ccaactgacc gacgaccaaa
      961 tcgaatggta cgcccgcgcc gccttgcgcg cccgacgttg ggacgagctg gcttccgtaa
     1021 tctcgcatat gcccgaaaaa ctgcaaaaaa gcccgacctg gctctactgg ctggcacgca
     1081 gtcgcgccgc aatgggcaac acgcaagagg cggaaaaact ttacaaacaa gcggcagcga
     1141 cgggcaggaa tttttatgcg gtgctggcgg gggaagagtt ggggcgcaaa atcgatacgc
     1201 gcaacaatgt gcccgatgcc ggcaaaaaca gcgtcctccg tatggcggaa gacggtgcag
     1261 tcaaacgcgc actggtattg ttccaaaaca gccgaaccgc cggcgatgcg aaaatgcgtc
     1321 gtcaggctca ggcggaatgg cgttttgcca cacgcggctt tgacgaagac aagctgctga
     1381 ccgccgcgca aaccgcgttc gaccacggtt tttacgatat ggcggtcaac agcgcggaac
     1441 gcaccgaccg caaactcaac tacaccttgc gctatatttc gccgtttaaa gacacggtaa
     1501 tccgccacgc gcaaaatgtt aatgtcgatc cggcttgggt ttatgggctg attcgtcagg
     1561 aaagccgctt cgttataggc gcgcaatccc gcgtaggcgc gcaggggctg atgcaggtta
     1621 tgcctgccac cgcgcgcgaa atcgctggca aaatcggtat ggatgccgcc cagctctaca
     1681 ccgccgacgg caatatccgt atggggacgt ggtatatggc ggacaccaaa cgccgcctgc
     1741 aaaacaacga agtcctcgcc accgcaggct ataacgccgg tccaggcagg gcgcgccgat
     1801 ggcaggcgga cacgcccctc gaaggcgcgg tatatgccga aaccatcccg ttttccgaaa
     1861 cgcgagacta tgtcaaaaaa gtgatgacca atgccgccta ctacgccagc ctcttcggtg
     1921 cgccgcacat cccgctcaaa cagcgtatgg gcattgtccc cgcacgctga cgtatcgatg
     1981 ccgtctgaaa cccgcccggt ctttcagacg gcatttttat cccgaacggc attgacggcg
     2041 aaccataaat ataagacaat ccggaaattg tttttcctgc tttttcaagc agcttgacac
     2101 ggcacaagcc gacccgttag gaggtgatgt ttccgtcacg gcgcgtatcc cgccgccgca
     2161 aggcacagcg atacggtaaa ccttcaacac cgtctgccct accctttcca ccgatatgat
     2221 gggcagatgc aacaaccgaa tttattaaag gaaataaaat gcctgcaatc cgcgtaaaag
     2281 agaatgaacc atttgaagtc gctatgcgcc gtttcaaacg cgccgtagaa aaaaccggcc
     2341 tgctgaccga gctgcgcgcc cgcgaagcct acgaaaaacc gactaccgaa cgcaaacgca
     2401 aaaaagcggc agccgtaaaa cgcctgcaaa aacgcctgcg cagccaacaa ctgccgccca
     2461 aaatgtacta aacgttcaag tacagattac aggtcagccc tgtgatatga ggacacaccg
     2521 caagacctgc tctgcggtgt gttttgcttt tcagacggca tcgaaacccg ccgtttccat
     2581 ccgacatccc agcgaggaca ttatgagcct gaaaatccgc cttaccgaag acatgaaaac
     2641 cgcgatgcgc gccaaagacc aagtttccct cggcaccatc cgcctcatca acgccgccgt
     2701 caaacagttt gaagtggacg aacgcaccga agccgacgat gccaaaatca ccgccatcct
     2761 gaccaaaatg gtcaaacagc gcaaagacag cgcgaaaatc tacactgaag ccggccgtca
     2821 ggatttggca gacaaagaaa acgccgaaat cgaggtactg caccgctacc tgccgcaaat
     2881 gctctccgcc ggcgaaatcc gtactgcggt cgatgccgcc gttgccgaaa ccggcgcggc
     2941 aggtatggcg gatatgggca aagtgatggg cgtattgaaa acccgcctcg ccggcaaagc
     3001 cgatatgggc gaagtcaaca aaattctgaa agccgtactg accgcctgat tgcccgaata
     3061 tcggacaaaa tgccgtctga agcccgtatc gcaggttcag acggcatttt caatgtccca
     3121 atatcgaatc ggcaggggca acacggtttc gatacaccga aacgggtttt gccgataaac
     3181 ggattccgtt tgcgccccat cggacaaaat gccgtctgaa acacgattcc gttcagacgg
     3241 catagattta tttgaccaat ttcaagcctt ttttggcggg ccggggcgcg gtttcggcag
     3301 aggtgttttc aggcggcgtg tcggggcgat acgcttccaa ttcaaacccc ataccttcgc
     3361 cggtctcccg tgcgaaaagg ctgaggacgt gtccgacagg tatccatata tcgtgcgcct
     3421 gtccgctgaa acgggcggaa aaactgatcc aatcgttgtc gatttgaagg ttttgcgtgg
     3481 cggtcgcgcc gatgttgagc ataatttcgt tgtcgcggac gtactgcatg gggacgcgcg
     3541 tgtgttcgtt gacccagaca aggatgtgcg gtgtgaggct gttgtcgctg caccattcgc
     3601 agagggcgcg gaggatgtaa ggtttggttg aggtgggcat gatgggttcc gtgtggtgtg
     3661 cctgaatgtg aaaatgcctg caaaacggcg ggttttgcaa gcattccggg cttatttgcg
     3721 catggctttt tcggcgggtg tcagtgcttc gataaaggct tcgcgctgga agatgcgctc
     3781 ggcgtatttg agcagcggcg cggcactttt gcccagtttg acatcgtagt ggtcgagccg
     3841 ccacagcagc ggagcaaggg cgacatcaat catagaaaaa tcttcgccga ggatgtattt
     3901 gcttttgctg aacgaagggg cgagcatggt cagaccgttg ccgatggctt cgcgcgcttt
     3961 tgcctgctcc ttattggtag cggcggggtt ttccaacact tggacgtggt tgaacaattc
     4021 tttttccata cggtacagca ccagccggcc cctgccgcgc ataacgggat cgccgggcat
     4081 aagctgcgga tgagggaagc gttcgtcgat gtattcgttg atgatgttgg actcgtgcag
     4141 caccaaatcg cgctcgacca gcacgggaac ttggttgtac gggttcatga cggcgaggtc
     4201 ttcgggcttg ttgtaaatat cgacgtcttt gatttcaaaa tccatacctt tttcgtacaa
     4261 aacgaagcgg cagcggtggc tgaaggggca ggtaatgccg gaatagaggg tcatcataat
     4321 aattgtcgct cctgtgtgat gcctgcaaaa cggct
//
LOCUS       DAXHTY010000112         4345 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXHTY010000112 DAXHTY010000000
VERSION     DAXHTY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.112"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..282)
                     /locus_tag="ACFNMK_001810"
     CDS             complement(<1..282)
                     /locus_tag="ACFNMK_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6365018.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYSGLTKTSTAL"
     gene            complement(279..425)
                     /locus_tag="ACFNMK_001811"
     CDS             complement(279..425)
                     /locus_tag="ACFNMK_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365019.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(442..780)
                     /gene="erpA"
                     /locus_tag="ACFNMK_001812"
     CDS             complement(442..780)
                     /gene="erpA"
                     /locus_tag="ACFNMK_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC6365020.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(912..1598)
                     /locus_tag="ACFNMK_001813"
     CDS             complement(912..1598)
                     /locus_tag="ACFNMK_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6365021.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            1786..2058
                     /locus_tag="ACFNMK_001814"
     CDS             1786..2058
                     /locus_tag="ACFNMK_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365022.1"
                     /translation="MKKTGKYLIYTAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2266..4194
                     /gene="dnaK"
                     /locus_tag="ACFNMK_001815"
     CDS             2266..4194
                     /gene="dnaK"
                     /locus_tag="ACFNMK_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC6365023.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKAFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         1325 a         1139 c          872 g         1009 t
ORIGIN      
        1 caacgccgta ctggtttttg ttaatccact atatctccgg gtaataatat ccaaacccaa
       61 atagctgaaa taatcgtgca actcttcccg tttcgcccaa gtgcattttt cctcaaccag
      121 cacttgaggt ttggaatcga gcgattcgat caatccttcc gccttttcct cgccgagatt
      181 ctccctgagg cactcccgga taagcccgcg atccacttgg ggcgggtagg aaacatatac
      241 atcgtacaaa tcatttcggt aatctttact catctcaatc ataagggaac aaatacgcct
      301 gataccgttc cgcccgttcc tttcgggaaa cccgattcat cttcaaacaa tcggattata
      361 tgtgaattca tcccgataat atattttcca aaagtaaaaa tgccgtctga acgttcagac
      421 ggcatcacac aaaccaagcg gtcatacgga aaacgacgat ccgcaaccgc aggttgtttc
      481 cgcattcgga ttgcggatga cgaattgcga accctgcaaa ctttccgtat agtcgatttc
      541 cgcaccgacc agatattgat agctcatcgg atcgaccaaa aagaccaaac cgtttttctc
      601 aatttcaaaa tcgtcgtcgt ttttgatttc gtcaaaagta aatccgtact ggaaacccga
      661 acagccgccg ccattgacaa aaacccgcaa tttcaaatcg ggattgtttt cttcggcaat
      721 caaatcggca actttggcac agcagctgtc agtaaaaata atagggcttt catccgacat
      781 ggtgttattc cttaatataa tattttctgc attctctctc aaaccccctt taaaggcaag
      841 caggcttcgc ctttgaaaaa ggaattgttc caatagataa gtttgaccgc ctgcaatttc
      901 aagccccaaa ctcaaatcgt cctgccgaac cactcgacac gcccgataat ctccacatca
      961 tcggtcaaat cgttcaaatt gatttcaaaa gcaggataag cctcgtttgc agaaatcaca
     1021 ttgataatcc cgcccggtac aatctgtaaa cgtttaacca gcagattttc attaatccgc
     1081 aacacataca gaccgtccct cggcgtattt tcaccatgat tgaccaaaat cgaatcgccg
     1141 tcattcaaaa ccccctccat cgaatccccc ttgacggaaa ttacagacag gtttttcgta
     1201 tcgcgggtaa cataattctc aatccaatgc cgtctgaacg ccattgtaaa taccggttcc
     1261 tcatgatcga caaactgccc gtatcccgca gccgcccgaa tatcatatct cggcacaaag
     1321 acaaactcgt ccgtatcgac ttcattgccc aaagtatcgt aagcaaggga tttttttggg
     1381 gcttcatccg gaaacggatt accctccccg gtcagcagcc aatcgatact acaccccttc
     1441 aactgcttga tttttttcaa tgtttcagac tttggcagac cgccttcatt ccatatcctg
     1501 ctgaagcccg caatcgtcat ttcaatatcg gatgcgattt ttgcctgcct cgcttcgctt
     1561 ttccataaaa aaaccagtct gtctttaaag gtttccataa atatccttcc actccccttc
     1621 attcaatcat ctaaagtaaa catgaaatat attttacctt agctatccaa aaaacggaac
     1681 aattatgata aactaaattt aaattatgat ttttcttact taatacataa tttttcagat
     1741 ttttccatag gtttaatcct gaattgacag aaaaaggata gaaaaatgaa gaaaaccggc
     1801 aaatatctta tctatactgc ggcatttacc gcattctgct ttgccttcca agaaaaccgt
     1861 tctgaagcca aacagcccga catcacttta tccgcatccc tgtgcgaaca attcaacata
     1921 ctgaacgcca aagatatgga tgcagaacaa gtctcccttt ccaaagaatg cgacatcatc
     1981 gagtcttcac acgactggga aaaagagtac ggcaacttga acgaacagga aatgctcgcc
     2041 ggcatcgtct atgaataaac ctgcctgcca tttgaaacat tatgcttgaa tgcattggag
     2101 ccaaatgtat taaatcaaat ataaaaccaa tatattcata aagttatata cttatagcca
     2161 tgctgtagct tgaaacagcc cgccgcccgc cctatttaca gcccatcggg acaaaatgtt
     2221 ccaacatatt tctcaaaata agcaaaatca aataggatta tccacatggc aaaagtaatc
     2281 ggtatcgact taggtacaac caactcttgt ttggccattt ccgaaaacgg tcaaaccaaa
     2341 gtgatcgaaa acgcagaagg cgcacgcacc acgccgtccg ttatcgctta tttggacggc
     2401 ggcgaaatcc tcgtcggcgc gcctgccaaa cgccaagccg taaccaacgc taaaaacact
     2461 atttacgccg ccaaacgttt gatcggccac aaatttgaag acaaagaagt ccaacgcgac
     2521 atcgaatcta tgccttttga aatcatcaaa gccaacaacg gcgacgcatg ggtgaaagcg
     2581 caaggcaaag agctgtctcc tcctcaaatt tccgcagaag tcctgcgtaa aatgaaagaa
     2641 gccgccgaag cttacttggg cgaaaaagta accgaagccg tgattaccgt tccggcctac
     2701 ttcaacgaca gccaacgtca agctaccaaa gacgcaggcc gtatcgccgg tttggacgtg
     2761 aaacgcatca tcaacgagcc gaccgcagcc gctttggcat tcggtatgga caaaggcgac
     2821 aacaaagacc gcaaagtagc cgtatatgac ttgggcggcg gtactttcga tatttccatc
     2881 atcgaaatcg ccaacctcga cggcgacaaa caatttgaag tattggctac caacggcgat
     2941 actttcttgg gcggtgaaga cttcgaccaa cgcttgatcg actacatcat tgacgagttt
     3001 aaaaaagaac aaggcattga tttgaaacaa gacgtcatgg ctctgcaacg tctgaaagaa
     3061 gctgccgaaa aagccaaaat cgaattgtcc agcggccagc aaaccgaaat caacctgccg
     3121 tacatcacca tggacgcaac cggcccgaaa cacttggcga tgaaaattac ccgcgccaaa
     3181 ttcgaaagcc tggttgaaga cctgattgcc cgctctatcg agccttgccg caccgcattg
     3241 aaagatgccg gtctgagcac tggcgacatc gacgacgtga ttttggtcgg tggtcaaagc
     3301 cgtatgccga aagtacaaga agccgttaaa gccttcttcg gcaaagagcc gcgcaaagac
     3361 gtgaaccctg acgaagctgt cgccgtaggc gcagcgattc aaggcgaagt gttaagcggc
     3421 ggccgcagcg acgtattgct gctggacgta acccctctgt ccttgggtat cgaaaccatg
     3481 ggcggcgtga tgaccaagct gattcaaaag aacacaacta ttccgactaa agcgtcgcaa
     3541 gtgttctcta ccgccgaaga caaccaaagc gcagtaacca tccacgtact gcaaggcgaa
     3601 cgcgaacgcg cttctgccaa caaatctttg ggtcagttca acttgggcga catcgcacct
     3661 gcaccgcgcg gtatgccgca aattgaagtt actttcgaca tcgacgccaa tggtatcttg
     3721 cacgtttctg ccaaagacaa aggcaccggt aaagcagcca acattaccat ccaaggttct
     3781 tcaggtttga gcgaagaaga aatcgaacgc atggtgaaag atgccgaagc caatgctgag
     3841 gaagataaaa agctgactga attggtcgct tcccgcaacc aagccgaagc cctgattcac
     3901 tccgtgaaaa aatctttggc cgactacggc gacaaactcg acgctgccga gaaagaaaaa
     3961 atcgaagccg cgctgaaaga agccgaagaa gccgtgaaag gcgacgacaa aaccgccatc
     4021 gatgccaaag ccgaagcact gggcacagcc agccaaaaac tgggggaaat ggtttacgct
     4081 caagcacaag ctgaagccca agcaggcgaa agcgaacaag ccaatgcttc tgcaaagaaa
     4141 gacgatgatg tcgtagatgc cgactttgaa gaagtaaaag acgacaaaaa ataattgatg
     4201 ccgtctgaaa aaagcgcgaa ccgtttgatt cgcgcttttt tcaattgaga taaaagacca
     4261 tagcataaca gaggttccaa attcatctac cgtgatattc ccaagcccaa gttctaactg
     4321 ttatatcggt tctctggaaa ttttt
//
LOCUS       DAXHTY010000113         4335 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXHTY010000113 DAXHTY010000000
VERSION     DAXHTY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.113"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(1..1001)
                     /locus_tag="ACFNMK_001816"
                     /pseudo
     CDS             complement(1..1001)
                     /locus_tag="ACFNMK_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(1012..1806)
                     /locus_tag="ACFNMK_001817"
     CDS             complement(1012..1806)
                     /locus_tag="ACFNMK_001817"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC6365024.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDPNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAAVSV"
     gene            2190..3524
                     /gene="gdhA"
                     /locus_tag="ACFNMK_001818"
     CDS             2190..3524
                     /gene="gdhA"
                     /locus_tag="ACFNMK_001818"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC6365025.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFEKQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3666..4040
                     /locus_tag="ACFNMK_001819"
     CDS             3666..4040
                     /locus_tag="ACFNMK_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365026.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            4084..>4335
                     /locus_tag="ACFNMK_001820"
     CDS             4084..>4335
                     /locus_tag="ACFNMK_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HFC6365027.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQ"
BASE COUNT         1157 a         1157 c         1015 g         1006 t
ORIGIN      
        1 ccggcatgac cagccaaacc ttaagccgcc tgctttcggc aaccgaccgt atcaagggca
       61 aatccataat ggaaaacgcc gtcgacaagg catcggcaac cgccgcatca tccgccatca
      121 cgctcatgct tttataacgc ggcgtacccg cgccggtccg cggatcgaac aaatgggtaa
      181 acctgcccgc cttgtccatc accgtcccat agccgcccga agtggcaaac gccttgtctt
      241 tcatcgtgat atttgccaac acgccttctt cgtcgtccag attgcagatg ccgacattcc
      301 aagtccgttt accgtttgta tcaaatcccc tgatttcgcc catatcgacc agcgcggcag
      361 gcacgccgtt tgctttaagc agcgcaacaa ccttgtccgt gatatagcct tgcgcgatgc
      421 cgttcaagga caaacccata cccttttcgg taaaacggat ttcacggtcg tcaaacgaga
      481 ctttgccgaa gccgacccgc ttcagggctt ccttcacgct attctccggc ggcggcgttt
      541 ccgcatcggg atgggcggaa aaataatcgg catacagttt ccacaaaacc tgcactgtcg
      601 gatcgaacgc gccttccgtc agcgcgtgaa tatcgcggca caggctcaac agttccaaaa
      661 aatccgccga cggcgaagtc agataaccgt cacggttcag gcggctgatc aggctgtctt
      721 cacggtaaag gctgaacaat tttttccaaa cgcgccactt ccgccaaaac cttgttgacc
      781 aaatccgccg cctgtctgtc gtccacaccg aacaggcgca gctccgcacc ggaacccagc
      841 gcgacacctt tccagaagaa cacattttca ttgcgttttt catctttatt gcgtttttca
      901 tcatcggcgg caaaaggatt cggcaggaaa gaaaccgccg cgcccgccgc cgcaacggcg
      961 gcaaccgtca gaaaacgcct gcgcccgaaa tgcctgtcca tatacccctc ctcagaccga
     1021 cactgccgcc ttgatgtgcg gatgcggatc gtagccttcc aactcaaaat cttcaaattt
     1081 gaaggcaaac aaatctttga cttcaggatt gattttcatc acaggcaagg cgcgcggttc
     1141 gcgttccaac tgcaatgcgg cctgctcgaa atggttgcgg tacaggtgcg cgtcgccaaa
     1201 cgtatggaca aactcgcccg cctccaatcc gcacacttgc gccatcatca tcgtcaacag
     1261 cgcgtagctg gcaatattaa acggcacgcc gaggaaaata tccgcgctgc gctggtaaag
     1321 ctggcaggac agtttgccgt cggcaacgta aaactgaaac agcgcgtggc agggcggcaa
     1381 tgccatttca tcgaccaaag ccggattcca cgccgacaca atcaggcggc gcgagtccgg
     1441 attcttcttg atttgttcca ccacattggc aatttggtcg atatggcgtc cgtcgggcgc
     1501 gggccaactg cgccattggt agccgtaaac cggtcccaag tcgccgtttt cgtccgccca
     1561 ctcgtcccaa atggaaacat tgttgtcctt cagatatttg atattgggat cgcctttgag
     1621 aaaccaaagc agctcgtgaa taatcgagcg cagatgtagc tttttggtcg tcagcagcgg
     1681 aaaacctttg cccaagtcaa aacgcatctg ataaccgaat acggagcgcg tgcccgtacc
     1741 ggtgcggtct gatttgtccg taccgttgtc gaggacgtgg cgcatcaagt ccaaataggc
     1801 tttcatagca gtctttcatc aaattaaacg gcgcatattg taacatttcc ggataatgcc
     1861 caaaacacgg atacaggcag gcaggattgt tggcaatttc agtccttttc cacagtaaaa
     1921 cccggtggga aaacaaaatt accttgattg gaatcagaaa atctagttta attacttaga
     1981 ataaaatttc aataatattt ttatttacga aattaattta tgattattga ttgattatcg
     2041 gcaacaaaca tcaaacatcg aaaacatgga aaaaataatg tcaacaattt ttgccaaatc
     2101 gggcttggca tcagaaaaaa ataggtttat attcccacct acaaatttgt tttcccatta
     2161 gtacactatc aaccaaaagg agtatccgaa tgactgacct gaacaccctg tttgccaacc
     2221 tcaaacaacg caatcccaat caggagccgt tccatcaggc ggttgaagaa gtcttcatga
     2281 gtctcgatcc gtttttggca aaaaatccga aatacaccca gcaaagcctg ctggaacgca
     2341 tcgtcgaacc cgaacgcgtc gtgatgttcc gcgtaacctg gcaggacgat aaagggcaag
     2401 tccaagtcaa ccggggctac cgcgtgcaaa tgagttccgc catcggtcct tacaaaggcg
     2461 gcctgcgctt ccatccgacc gtcgatttgg gcgtattgaa attcctcgct tttgaacaag
     2521 tgttcaaaaa cgccttgacc accctgccta tgggcggcgg caaaggcggt tccgacttcg
     2581 accccaaagg caaatccgat gccgaagtaa tgcgcttctg ccaagccttt atgaccgaac
     2641 tctaccgcca catcggcgcg gacaccgatg ttccggccgg cgacatcggc gtaggcgggc
     2701 gcgaaatcgg ctacctgttc ggacaataca aaaaaatccg caacgagttt tcttccgtcc
     2761 tgaccggcaa aggtttggaa tggggcggca gcctcatccg tcccgaagcg accggctacg
     2821 gctgcgtcta tttcgcccaa gcgatgctgc aaacccgcaa cgatagtttt gaaggcaaac
     2881 gcgtcctgat ttccggctcc ggcaatgtgg cgcaatacgc cgccgaaaaa gccatccaac
     2941 tgggtgcgaa agtactgacc gtttccgact ccaacggctt cgtcctcttc cccgacagcg
     3001 gtatgaccga agcgcaactc gccgccttga tcgaattgaa agaagtccgc cgcgaacgcg
     3061 ttgccaccta cgccaaagag caaggtctgc aatactttga aaaacaaaaa ccgtggggcg
     3121 tcgccgccga aatcgccctg ccctgcgcga cccagaacga attggacgaa gaagccgcca
     3181 aaaccctgtt ggcaaacggc tgctacgtcg ttgccgaagg tgcgaatatg ccgtcgactt
     3241 tgggcgcggt cgagcaattt atcaaagccg gcatcctcta cgccccggga aaagcctcca
     3301 atgccggcgg cgtggcaact tcaggtttgg aaatgagcca aaacgccatc cgcctgtctt
     3361 ggactcgtga agaagtcgac caacgcctgt tcggcatcat gcaaagcatc cacgaatcct
     3421 gtctgaaata cggcaaagtc ggcgacacag taaactacgt caatggtgcg aacattgccg
     3481 gtttcgtcaa agttgccgat gcgatgctgg cgcaaggctt ctaagcaaac gccgccgctc
     3541 cgcaaacaaa atgccgtccg aaccgcaaat gctgttcaga cggcatttcc ttatccgccc
     3601 gttcaaatcg ggtgatacta tttgtatcca gaattgttgg atagtttggg agtggagagg
     3661 aagaaatgat tatggaccaa tactatatgg agttaaagaa taagttatct aacagaccaa
     3721 tcctactgga taatactaat gattttcttt ttgttttagt aaatacagtg aaagctatga
     3781 tagaaaatac tgataaaagt cagctttcag agttagataa aattttagac ggtgtcacaa
     3841 gtcaagaatt gaaacttgcc tatgattttt gtcaagggaa attcggtcaa gcaggttttt
     3901 cttatcgtag gcacccgaat tatttttatt tgtccagttt aattgcgact tttccagaat
     3961 ttgagttgtc taaagcagac cgagattacc tcaaaggaat cataaatttt gataactacc
     4021 ttttatatga gttagattag tctttaatca cctcaaaacc ctatttattc aaaaggagaa
     4081 aaaatgattg agctgaaatt agtagatgag agcagttttc aggcagtgct ggatttgaaa
     4141 atatctgaag ctgatgaacg agcacgtttt gtggctccaa atgtacgctc tttagctgac
     4201 gcatggctct accgggaaaa tgaagatgtg tttccgaggg caatctattg ggataagcaa
     4261 gtggttggct ttctcctgct ggaaatagac aaggatgaag cagaatactt tatctggcgg
     4321 ataatgattg gtcag
//
LOCUS       DAXHTY010000114         4276 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXHTY010000114 DAXHTY010000000
VERSION     DAXHTY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.114"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            433..957
                     /gene="nspA"
                     /locus_tag="ACFNMK_001821"
     CDS             433..957
                     /gene="nspA"
                     /locus_tag="ACFNMK_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC6365028.1"
                     /translation="MKKALATLIALAIPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            1093..1482
                     /locus_tag="ACFNMK_001822"
     CDS             1093..1482
                     /locus_tag="ACFNMK_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6365029.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1560..2390
                     /locus_tag="ACFNMK_001823"
     CDS             1560..2390
                     /locus_tag="ACFNMK_001823"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC6365030.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIRESSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2405..3862
                     /locus_tag="ACFNMK_001824"
     CDS             2405..3862
                     /locus_tag="ACFNMK_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC6365031.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3903..>4276)
                     /locus_tag="ACFNMK_001825"
     CDS             complement(3903..>4276)
                     /locus_tag="ACFNMK_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365032.1"
                     /translation="ENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDTIPLYIF
                     DLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEKALKALE
                     KYPEIYQRFQHFFPFVELPLF"
BASE COUNT         1012 a         1394 c          967 g          903 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaaaa ccgtatgcct gcacctgcaa
      181 caatccgaca gataccgctg ttttttccaa accgtttgca agtttcaccc atccgccgcg
      241 tgatgccgcc accaccattt aaaggcaacg cgcgggttaa cggctttgcc gtcggcaaag
      301 cagccggata ccgctacgta tcttgaagta ttgaaaatat tacgatgcaa aaagaaaatt
      361 taagtataat acagcaggat tctttaacgg attcttaaca atttttctaa ctgaccataa
      421 aggaaccaaa atatgaaaaa agcacttgcc acactgattg ccctcgctat cccggccgcc
      481 gcactggcgg aaggcgcatc cggcttttac gtccaagccg atgccgcaca cgcaaaagcc
      541 tcaagctctt taggttctgc caaaggcttc agcccgcgca tctccgcagg ctaccgcatc
      601 aacgacctcc gcttcgccgt cgattacacg cgctacaaaa actataaagc cccatccacc
      661 gatttcaaac tttacagcat cggcgcgtcc gccatttatg acttcgacac ccaatcgccc
      721 gtcaaaccgt atctcggcgc gcgcttgagc ctcaaccgcg cctccgtcga cttgggcggc
      781 agcgacagct tcagccaaac ctccaccggc ctcggcgtat tggcgggcgt aagctatgcc
      841 gttaccccga atgtcgattt ggatgccggc taccgctaca actacatcgg caaagtcaac
      901 actgtcaaaa acgtccgttc cggcgaactg tccgccggtg tgcgcgtcaa attctgatat
      961 gcgccttatt ctgcaaaccg ccgagccttc ggcggttttg ttttctgcca ccgcaactac
     1021 acaagccggc ggttttgtac gataatcccg aatgctgcgg cttctgccgc cctatttttt
     1081 gaggaatccg aaatgtccaa aaccatcatc cacaccgaca aagcccccgc cgccatcggc
     1141 gcgtacagcc aagccgtccg agcaggcgac accgtttaca tgagcggtca aatccccctc
     1201 gatcccgcca cgatgaccgt cgtcggcaac ggcgatttcc gcgccgaagc gcgccaagtg
     1261 ttccaaaacc tgcaagccgt cgccgaagcg gcaggcggca cgctggccga catcgtcaaa
     1321 ctcaacgcct acctgaccga cttgggcaat tttgccgtct tcaacgaagt gatggcggaa
     1381 tttattgccg agcccttccc cgcccgcgcc gccgtcggcg ttgcctccct gccaaaaggc
     1441 gtgcaggtcg aagccgaagc cgtcctcgtt ttgaacgcat aattccacgt tcggactgca
     1501 tgatatttca aaaccatgcc gtccgaaccg caacactttc ccacttcaca acatctgcaa
     1561 tggcacatta cgcaatcggc gacatacaag gctgtttcga cgaactgacc gcgctgctcg
     1621 gcaaaatcgg tttcaaccac ggcacggaca ccctctggct gacgggcgac atcgtcaacc
     1681 gcggcccgaa atccctcgaa acgctgcaat tctgcatccg gcacgaaaac agcgtgcaaa
     1741 tcgtcctcgg caaccacgac ctgcacctgc tcgctgtcgg ctgcggcgaa ggcgcgccca
     1801 aacgcagcga cacaatcgaa cccatactca aacaccccga cggaaaaaaa atgctcgact
     1861 ggctgcgtgc gcaaccgctg ttgatacgcg agagcagccg cgtgatggta cacgccggca
     1921 tcctgccgca atggcgcatc gccaaagccg aatcgctcgc tagagaagcc gaagccgaac
     1981 tgcgcggcaa aaaatacgtc aaattcttct ccaaaatgta cggcaacaaa ccggccgcgt
     2041 gggacgaagg tttggaaggc tatgcccgcc tgcgcttcat cgtcaacgcc ttcacgcgga
     2101 tgcgcgccct gacctttaaa aacgaactgg atttcgacta caaatccaca gtgaaaaaaa
     2161 tgccgcctta cctgcgcccg tggttcaaag cccccgaccg gcaaaacctc gaccgcacca
     2221 tcatcttcgg acactggtcc tcgctgggct acacgaatgc cgacaacgtc atctcgctgg
     2281 acaccggcgc gctgtggggc ggacagctga ccgccgtcaa cctcgaaacg gaagaaatta
     2341 cccaagtcca agccgccggc ggcatagact ggaaaagctt cgcaaaataa cggaccgccc
     2401 cactatgcac aaaatcctgc ccatcgccca cgtcctctcc cgactgggta tgctgttttc
     2461 ctttatcctg ctgatacccg ccgccctctc ctacgccttt tcggacggcg cgtacaccgc
     2521 cttcgccacc accgcgaccg ttaccctttc cggctcgtgc atcgtccggc tcgccaccct
     2581 ccggttcagg cgcgaactgc gcccgcgcga cggcttcacc ctcgtcctga tgttgtggct
     2641 ggcgtttgcc gctatggcgg cgatgccgat gtacctgtat ttcccgaata tgggctttac
     2701 cgacgcattt tttgaatcga tgtcgggact gaccaccacc ggcgcgaccg tcatccccca
     2761 cgtcgacggg ctcgccccct ccgtcaactt ttggcggcat atgctcaact ggctgggcgg
     2821 gatgggcatc atcgtccttg ccgtcgccat cctgcctatg ctcggcgtag gcggcacgca
     2881 gttattcaaa gccgaaatcc ccggcattga caaagaaagc aaaatgtcgc cgcgcatttc
     2941 ccaagtggcg aaaaaacttt ggttcggcta caccctgatc accatcctcg cggcagcctg
     3001 cctgcatttt gccgggatgg gctggttcga tgccgtctgt cacgcgatgg cgaccctctc
     3061 gctgggcggg ttttccaccc acgatgccag catagcttat tacaactccc ccctcatcga
     3121 agcggtcatc atcgttttca ccattgtcgg cggcatcaat tttgccaacc atttcgccgc
     3181 cctcaacagc cgcagcctca aaacctattg gaaagacgaa gaatgccgga cgatgctgct
     3241 gctgctctcc ggcagcatcc ttgccgccgc cctgtacctg tggcacaccg gctattacgc
     3301 cggttttacc gaatccctgc gctacaccgc cttcaacttc gtctccatcg gactggcaaa
     3361 cgggctggcc aacaccgact tcgcacaatg gccgctcctg atttccctgt ggatgttttt
     3421 cctcgccaac atcctcgcca actccggctc gaccggcggc ggcatcaaaa ccatacgcgc
     3481 cctcgtcctg ttcaaattca gcctgcgcga aatgatggtg ctgctgcacc ccaaagccgt
     3541 ccgcaccgtc aaaatcagcg gcaaggccat acccgaccgc ctcgcgctga ccgttatgtc
     3601 cttcatcttc atctacttta tgaccgtcgt cctcttcagc ttcctgctga tggcgagcgg
     3661 tatggaattt accaccgcct tcaccgccgt catcgcctgc atcaccaacg ccggccccgg
     3721 cctgggcgaa gtcgggcccg ccggcaatta cgccggtttg gacgtgatgc agaaatggat
     3781 ctgcgttacc gccatgctct tgggcaggct ggaaatcttc accgtcttca tcctcttcac
     3841 ccccgcctac tggaagaaat aaaaaatgcc gtctgaaaac ttttcagacg gcatattgtc
     3901 ttttaaaaaa gcggaagctc tacaaaagga aagaaatgct gaaacctctg ataaatttca
     3961 ggatatttct ctaaggcttt taatgctttt tcttttgaaa taggcggatc atagacatct
     4021 atccccctta agaaggcaat gcccgttaat gcattttttt gatcatttga aatgtaacca
     4081 taattaacga ctccaattac atcataaatt tcccctatac tcttatcaaa atcaatcaaa
     4141 tcaaaaatat acaatggaat tgtatcaata ggagtatctc tgacaactat atccaaccat
     4201 aatttaaatt cagaaagatt aatagcatca tagaacatac agactattgc aaatcctaaa
     4261 tcttcacaat tttctt
//
LOCUS       DAXHTY010000115         4162 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXHTY010000115 DAXHTY010000000
VERSION     DAXHTY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.115"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            102..998
                     /gene="glyQ"
                     /locus_tag="ACFNMK_001826"
     CDS             102..998
                     /gene="glyQ"
                     /locus_tag="ACFNMK_001826"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC6365033.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAVEEASLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLIKK"
     gene            1115..3178
                     /gene="glyS"
                     /locus_tag="ACFNMK_001827"
     CDS             1115..3178
                     /gene="glyS"
                     /locus_tag="ACFNMK_001827"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC6365034.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLIAGA
                     AEFTVYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAALGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3195..>4162
                     /locus_tag="ACFNMK_001828"
     CDS             3195..>4162
                     /locus_tag="ACFNMK_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6365035.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIARRFQEQDGRIRILAQPRNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFA"
BASE COUNT         1067 a         1211 c         1060 g          824 t
ORIGIN      
        1 atgtatgccg tgttttcaaa tcagtctgtc tttgagataa acctatccaa acagcaatgg
       61 aaacagtaaa ataaaccctt tattttatcg aatcataagc catgcttacc ttccaacaaa
      121 tcatcttcaa actgcaaaca ttctgggcag acaaaggctg caccgtcatc caacctttcg
      181 acatggaagt cggcgcaggt acttcccacc ccgccacctg cctgcgcgca ctcggccccg
      241 agccttggtt tgccgcctac gtccaaccca gccgccgccc caaagacggc cgctacggcg
      301 acaaccccaa ccgcctgcaa cactattacc aattccaagt cgcccttaaa cccgcccccg
      361 ccaatatcca agacctttat ctcgactccc tgcgcgaatt gggcatcgat cccaaagtcc
      421 acgacatccg ctttgtcgaa gacgactggg aaaaccccac cctcggtgca tggggtttgg
      481 gctgggaagt ctggctcaac ggcatggaag taacccagtt tacctatttc caacaagtcg
      541 gcggcattga ctgcacgcct gtactcggcg aaattaccta cggcatcgaa cgcttggcga
      601 tgtacctgca aggcgtagaa aacgtctatg acctcgtttg ggcaaaaacg ctggacggga
      661 atacagtaac ttacggcgac gtgtaccacc aaaacgaagt cgagcaatcc acctacaact
      721 tcgaatacag cgatgccgac tggctgctgc gtcagttcaa cgactacgaa gcgcaagcca
      781 aacgcctgct cgccgttgaa gaagcaagcc ttgcgctgcc cgcctacgag ctggttctca
      841 aagcagggca tacgttcaac cttttagacg cacgcggcgc gatttccgta accgaacgcg
      901 ccacctacat cgggcgcatc cgcgcgttga gccgcgccgt ggcgcaaaaa tatgtcgaaa
      961 gccgcgaaaa actgggcttc ccgttgatta aaaaataacg atacgtcgtc tgaaacaggt
     1021 tttcagacgg ccttttttgc gtatccatta atacaggccg tacggtatat gcaggcttta
     1081 taagcagggc ctacggacaa acagaaagtc tcatatgaca acccaaaccc ttttaatcga
     1141 actccttacc gaagaactcc cgcccaaagc cctcaataat ctgggcaacc attttgccgc
     1201 ctccgttgcc gaaggcttgg aaaaagcgca actgatagcc ggtgcagctg aatttaccgt
     1261 ctacgcttct ccgcgccgtt tggcggttca agtcaaaaac gtcaaagccg ttcaagccga
     1321 ccaaaaaatc gtgaaaaaag gccctgccgt ggcgaatgcc atgaaagacg gtgcgccgac
     1381 caaggctttg gaaggtttcg cacgcggtgc gggcgcgaaa atcgaagact tgaccatcgt
     1441 ccacgacggc aaacaggatg tgtacgccta cgaatacgtc caaaccggca aaccgctggg
     1501 cgaacttttg gaagacatta tcaatcaagc cgttaagaaa ctgccgattc cgaaagtgat
     1561 gcgttggggc agcagcacgt ttaccttcgt gcgccctgtt cacgggctga ttgtgctgca
     1621 cggcggcgac atcgtaaacg tcagcgtttt gggtctgcaa agcagcaatc aaaccttggg
     1681 acaccgcttc ttgtccaacg gcgaaatcac cattgaaaac gccgacagct acgccgaaca
     1741 aatgcgcggg caaggcaaag tcgtcgcttc gtttgccgag cgcaaagccg cgattcagac
     1801 ggcattggaa gggcaggcgc gcagtctgaa cgccgccgtc gccgccgatg aagcattgtt
     1861 ggacgaagta accgcgctgg tcgaatggcc tgttgttttg gaagccggtt ttgaggaaca
     1921 tttcctcgcc gtgccgcagg aatgcttgat tttaacgatg cagcaaaacc aaaaatactt
     1981 tccgctgctc gaccaaaacg gcaagctgat gaaccgcttc ctgctggttt ccaacctgca
     2041 aaccgaagac ccgtcgcaca tcatccgagg caacgaacgc gttttgcgcg cgcgcctgtc
     2101 tgatgctgag ttcttctaca aacaagacca aaaagcgact ttggaaagcc gcctgccgaa
     2161 attgtccagc gtggtgtacc acaacaaaat cggctcgcaa gccgaacgca tcgaacgcct
     2221 gcaaagcatt gccgcccaca tcgccaaagc attgggcgcg gatgccgccg ctgccgaacg
     2281 cgccgcgcgt cttgccaaag ccgacttggt aaccgaaatg gttggcgagt tccccgaact
     2341 gcaaggcacg atgggcaaat actatgcacg tttggacggc gaaaccgaag aaatcgccga
     2401 agccatcgag cagcactatc agccgcgttt tgccggcgac aagctgcccg aaagcaaaat
     2461 tgccgccgcc gtggcactgg cggacaagct ggaaaccctg gtcggcattt ggggcatcgg
     2521 tctgattccg accggcgaca aagaccccta cgccctgcgc cgcgccgcct tgggtatttt
     2581 gcggatgctg atgcagtacg gtttggacgt gaacgaactg attcagacgg cattcgacag
     2641 cttccccaaa ggtttgctca acgaaaaaac gccgtctgaa accgccgact ttatgcaggc
     2701 gcgccttgcc gtgttgctgc aaaacgatta tccgcaagac atcgtcgccg ccgtactcgc
     2761 caaacagccg cgccgtttgg acgatttgac cgccaaactg caagccgttg ccgcgttcaa
     2821 acagctgccc gaagccgccg cgctcgccgc cgccaacaaa cgcgtgcaaa acctgctgaa
     2881 aaaagccgat gccgcgttgg gcgcggttaa cgaaagcctg ttgcaacagg acgaagaaaa
     2941 agccctcttt gccgccgcgc aaggcttgca gccgaaaatc gccgccgccg tcgccgaagg
     3001 caatttccaa accgccttgt ccgaactggc ttccgtcaaa ccgcaagtcg atgcattctt
     3061 tgacggcgtg atggtaatgg cggaagatgc cgccgtaaaa caaaaccgcc tgaacctgct
     3121 gaaccgcttg gcagagcaaa tgaacgcggt agccgacatc gcgcttttgg gcgagtaacc
     3181 gttgtacagt ccaaatgccg tctgaagcct tcagacggca tcgtgcctat cgggagaata
     3241 aattgcagcc tttagtcagc gtattgattt gcgcctacaa cgtagaaaaa tatttcgccc
     3301 aatcattagc cgccgtcgtg aatcagactt ggcgcaactt ggatattttg attgttgatg
     3361 acggctcgac ggacggcaca cttgccattg cccggcgttt ccaagaacag gacggccgca
     3421 tccgcatcct cgcgcagccg cgcaattccg gcctgattcc ctctttaaac atcgggctgg
     3481 acgaattggc aaagtcagga atgggggaat atattgcacg caccgatgcc gacgatattg
     3541 ccgcccccga ctggattgag aaaatcgtgg gcgagatgga aaaagaccgc agcatcatcg
     3601 cgatgggcgc gtggctggaa gttttgtcgg aagaaaagga cggcaaccgg ctggcgcggc
     3661 atcacaggca cggcaaaatt tggaaaaagc cgacccgaca cgaagatatt gccgactttt
     3721 tccctttcgg caaccccata cacaacaaca cgatgattat gaggcgcagc gtcattgacg
     3781 gcggtttgcg ttacaacacc gagcgggatt gggcggaaga ttaccaattt tggtacgatg
     3841 tcagcaaatt gggcaggctg gcttattatc ccgaagcctt ggtcaaatac cgccttcacg
     3901 ccaatcaggt ttcatccaaa tacagcgtcc gccaacacga aatcgcgcaa ggcatccaaa
     3961 aaaccgccag aaacgatttt ttgcagtcta tgggttttaa aacccggttc gacagccttg
     4021 aataccgcca aataaaagca gtagcgtatg aattgctgga gaaacatttg ccggaagaag
     4081 attttgaacg cgcccgccgg tttttgtacc aatgcttcaa acggacggac acgctgcccg
     4141 ccggcgcgtg gctggatttc gc
//
LOCUS       DAXHTY010000116         4162 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXHTY010000116 DAXHTY010000000
VERSION     DAXHTY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.116"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(17..955)
                     /locus_tag="ACFNMK_001829"
     CDS             complement(17..955)
                     /locus_tag="ACFNMK_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365036.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVGRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            1310..2422
                     /gene="nadA"
                     /locus_tag="ACFNMK_001830"
     CDS             1310..2422
                     /gene="nadA"
                     /locus_tag="ACFNMK_001830"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC6365037.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKTEHLDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKQFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA"
     gene            2474..3982
                     /gene="nadB"
                     /locus_tag="ACFNMK_001831"
     CDS             2474..3982
                     /gene="nadB"
                     /locus_tag="ACFNMK_001831"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC6365038.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDQ
                     NNNDLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDVQTES
                     GAACGLTVLDRRTQETYNIRASHTVLAGGGLGQIYASTTTPPECTGDAIAMAIRAGCT
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLTAQT
                     IADGQAFQTATSQRSSENTSAEADIFSDDLQNAFSRPTLQAFNQRHLGIIRNDTGLRC
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT          955 a         1230 c         1172 g          805 t
ORIGIN      
        1 gtcgtctgaa aacgggctaa cggctgccca gcggcaatcc gatgtccgaa atcagcctgt
       61 cgggcaggac gtcgtcgcgg aagcggcaaa gctgcgcggg acggcctttg ctgcccgata
      121 cgccggtatc cgacggctcg ataaggtttt gctgctgaat ctggcggcgg aagttttgct
      181 tgtgcagcaa tcttccgctg atggcttcga cactgttttg cagttgcagc agcgtgaatt
      241 cgggcggcat cagttcaaaa atcacggggc ggtatttgat tttggcgcgc aggcgggaca
      301 gggcggtcgc caatacgcgg cggtggtcgt ggcgcatggg ctgccctgtg agcgcgaaat
      361 cgaagtttgc ctgcggctcg gcggcttcca ctatcaggcc gctttcatac agcatttcat
      421 agcgttgcaa aacgtattct tccgaccagt tttccggttc gaccccccaa cacaaatgaa
      481 tgcgcttgag ccgcatttga cgcacttcct ccgtgtccgc cgagtttgcc caaatgcgca
      541 gtcggccgac gacggcgtcg cgctgtccgc cgtcggtgcg caagtcttcc cacgggaaat
      601 agccgtagca gtcctgccat ttcgcatccg ggtgcaggat gctgtcggct gcctcgcgca
      661 ccagccccaa atagctgacg tacaagacgg gcatgccgtg ttcgttgcgg cggtgggtat
      721 cgacaaaggt gtaaagctgt tccacatagc ccataggctg cgaagtctgc ttggcaaccc
      781 acagcttcac gcccgcctgc agggaattgc gcagagggga gagcgggccg ttgggcagga
      841 gcgtgccttg ggcgacggtt aatacccgca ggccgccgtc ggtaacagca atcaatacgg
      901 gaaccagctc gacgatgctt tgcggcgggg cttcggcttc ggggtaggca tccatgctcg
      961 gctttctgcg ggtggaaaag gctttattgt atagtggatt aacaaaaatc aggacaaggc
     1021 gacgaagccg cagacagtac aagcagtacg gaaccgattc acttggtgct tgagcacctt
     1081 agagaatcgt tctctttgag ctaaggcgag gcaacgccgt actggttttt gttaatccac
     1141 tataaaccaa agcggtttgt cggaacgaag cgggtcggtt ttacggtatc gcaaacctgt
     1201 tttcagacga ccttgcatat tatactcaag ttgagcataa tgtaaaaaca gtttgaaaaa
     1261 cagacggcaa accttcatgc cgtctgaaaa ccagctacaa ggaaacatca tgcaaaccgc
     1321 cgcccgccgc tcgttcgact acgatatgcc cctcatccaa acgccgactt ccgcctgcca
     1381 aatccgtcag gcgtgggcga aggttgccga cacgcccgac cgcgagacgg caggtcgtct
     1441 gaaagacgaa atcaaggcct tgctgaagga gaaaaacgcg gttttggtgg cgcattatta
     1501 cgttgatccg ctgattcaag atttggcttt ggaaacgggc ggatgcgtgg gcgattcgtt
     1561 ggagatggcg cgttttggcg cggaacacga agccggtaca ttggtggtgg cgggtgtgcg
     1621 attcatgggc gagagcgcga aaatcctctg ccctgaaaaa acggtgctga tgcctgattt
     1681 ggaggcggaa tgttctttgg atttgggttg cccggaagaa gcgttttcag cgttttgcga
     1741 ccaacacccc gaccgcacgg tggtggtgta cgccaacact tccgccgccg tgaaagcgcg
     1801 tgccgattgg gtggtaacgt cttcggtggc gttggaaatc gtgtcgtatc tgaaatcgcg
     1861 cggcgagaaa ctgatttggg gacccgaccg ccacctcggc gactacatcc gccgcgaaac
     1921 gggcgcggat atgctgttgt ggcagggttc gtgcatcgtc cacaacgaat tcaaagggca
     1981 ggagctggcg gcgttgaaga cggaacacct cgacgcggtg gtgctggttc atcccgaatc
     2041 gccgcaaagc gtcatcgaac tgggcgacgt ggtcggctcg accagcaaac tgctcaaagc
     2101 cgccgtatcg cgtcctgaaa aaaaattcat cgtggcgacc gatttgggca tcctgcacga
     2161 aatgcaaaag caggcgcccg acaaacaatt tatcgccgcg ccgacggcgg gcaacggtgg
     2221 aagctgcaaa agctgcgcgt tctgcccgtg gatggcgatg aattcgctgg gcggcatcaa
     2281 atacgccctg acaagcggac gcaacgaaat cctgttggac agaaagctgg gcgaagccgc
     2341 caaactgcct ttgcagcgta tgctcgattt cgcggcaggg ctgaagaaaa aagatgtgtt
     2401 caacggcatg ggccccgcct gatttgccgt ccatattgcc gtttcagacg acctctcaaa
     2461 caaggacaac accatgcaaa ccgattgcga cgtattgatt gccggaaacg ggctggcggc
     2521 actgacgctc gccctgtcgc tgcctgaatc gttccgcatc gtcattttgt gcaaaaaccg
     2581 gctggacgac accgccagcc gtcatgcgca aggcgggatt gcggcggcgt ggtcgggaga
     2641 ggacgacatc gaaaaacacg ttgccgatac cttggaagcg ggcgcgggct tgtgcgacga
     2701 agccgccgtc cgcaccatcc tgtcgcaggg caaaccggca atcgaatggc tgcttgcgca
     2761 aggcgtggcg ttcgaccaaa ataataacga cctgcacctg acgcgcgaag gcgggcatac
     2821 ctgccgccga atcgcccacg tcgccgatta cacgggcgaa gccgtcatgc agagcctgat
     2881 tgcccaaata cgccgccgcc cgaacatccg cgtttgcgag cggcagatgg cgttggacgt
     2941 tcaaaccgaa tcgggtgcag cgtgcggact gaccgtcctc gaccgccgaa cgcaagaaac
     3001 ctacaacatc cgtgccagcc ataccgtact cgcaggcggc ggcttgggac agatttacgc
     3061 ctccaccacc acgccgcccg aatgcacggg cgacgccatc gccatggcga tacgcgcagg
     3121 ctgcacagtc gagaacctcg aatttatcca attccacccc acaggtttgg caaggccgtc
     3181 tgaaaacgga cgcaccttcc tgatttccga agccgtgcgc ggcgaaggcg gcatcctgac
     3241 caaccaatcg ggcgaacggt ttatgccgca ttacgaccgc cgcgccgaac tcgcaccgcg
     3301 cgacatcgtt gcccgcgcca tcgcagccga aatcgccaaa caaacgcaag acttcgtctc
     3361 gctcgacatc agccatcaac ccgcagcgtt cgtccgccgg catttcccat ccatccatcg
     3421 acactgttta tcccaatgcg gcctagacat cacgcgccaa gccatccccg tccgccccgt
     3481 gcaacactac acctgcggcg gcatccaaac cgacccctgc ggcagaacct ccctgccgca
     3541 gctctacgcc ttaggcgaaa ccgcctgcac cggcttgcac ggagccaacc gcctcgccag
     3601 caactccctg ctcgaatgtg tcgttaccgc cagacttacc gcccaaacca tcgcagacgg
     3661 acaagcattc caaaccgcaa cgtcccaaag gtcgtctgaa aacacctccg ccgaagcaga
     3721 catcttttca gacgacctcc aaaacgcatt cagccgcccc accctgcaag cgttcaacca
     3781 acgccatctg ggcatcatac gcaacgatac cggcctacgc tgcgccatcg cccagctgcg
     3841 gctttggaag caaaaccaaa ccgaaccgca caccgcgtcc gaatacgaaa accgtaacct
     3901 gctcgaatgc agcctcgccg tcgcccaagc cgcatacagg cggagacaga acatcggcgc
     3961 gcattttaat agtgattgtt aaggttggct ggttagaaaa aatcgttcaa atgaatttta
     4021 atagcatggt taagagattg gcattcagtt tttagggttg cgtaagttgg gttatcaatc
     4081 tgttctaaaa gcagaaaggc cgagaccttt gcaaaatccc ccaaaattcc ctaaattccc
     4141 accaagacat ttaggggatt tt
//
LOCUS       DAXHTY010000117         4161 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXHTY010000117 DAXHTY010000000
VERSION     DAXHTY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.117"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            57..480
                     /locus_tag="ACFNMK_001832"
                     /pseudo
     CDS             57..480
                     /locus_tag="ACFNMK_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            586..1149
                     /gene="pgsA"
                     /locus_tag="ACFNMK_001833"
     CDS             586..1149
                     /gene="pgsA"
                     /locus_tag="ACFNMK_001833"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC6365039.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1229..1304
                     /locus_tag="ACFNMK_001834"
     tRNA            1229..1304
                     /locus_tag="ACFNMK_001834"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1262..1264,aa:Gly,seq:gcc)
     gene            1330..1405
                     /locus_tag="ACFNMK_001835"
     tRNA            1330..1405
                     /locus_tag="ACFNMK_001835"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1363..1365,aa:Gly,seq:gcc)
     gene            1429..1504
                     /locus_tag="ACFNMK_001836"
     tRNA            1429..1504
                     /locus_tag="ACFNMK_001836"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1462..1464,aa:Gly,seq:gcc)
     gene            1526..1601
                     /locus_tag="ACFNMK_001837"
     tRNA            1526..1601
                     /locus_tag="ACFNMK_001837"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1559..1561,aa:Gly,seq:gcc)
     gene            1627..1700
                     /locus_tag="ACFNMK_001838"
     tRNA            1627..1700
                     /locus_tag="ACFNMK_001838"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1659..1661,aa:Cys,seq:gca)
     gene            1803..1892
                     /locus_tag="ACFNMK_001839"
     tRNA            1803..1892
                     /locus_tag="ACFNMK_001839"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1837..1839,aa:Leu,seq:taa)
     gene            1996..3048
                     /gene="sohB"
                     /locus_tag="ACFNMK_001840"
     CDS             1996..3048
                     /gene="sohB"
                     /locus_tag="ACFNMK_001840"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC6365040.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3099..3965)
                     /locus_tag="ACFNMK_001841"
     CDS             complement(3099..3965)
                     /locus_tag="ACFNMK_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFC6365041.1"
                     /translation="MWKMLKHIAQTHRKRLIGTFSLVGLENLLMLVYPVFGGRAINAV
                     IAGEVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLECDNHFIRKGDRRQLYRHYGLVARLRVLISNREAFGYLCVG
                     TAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IGGLERNIKAGT"
     gene            complement(3976..4092)
                     /locus_tag="ACFNMK_001842"
                     /pseudo
     CDS             complement(3976..4092)
                     /locus_tag="ACFNMK_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
BASE COUNT         1051 a          997 c         1077 g         1036 t
ORIGIN      
        1 aatttaatcc actatattat cgctgggtat ggtttgccgc cataagctgt ctgctgttgt
       61 ccgccgtttt tattgcacct tacctgacgg catttcacga acaagaaaaa acattagaat
      121 atgcggaatt gaccgttacc gcccccaacc gcagcggacg ggcaatcaaa ctggatgcag
      181 acgggcagca ttaccggctt tcctgctatg ggttcgacag tttgtgcaca ggcggcaata
      241 tcggcagaac catcagggcg gagcaggtta gagccgtttt aagcgaaacc gtcggcaaag
      301 gttttttaaa cggcgttctg ttggaatacc gcaacagtgg cagtgtctat aagcaataaa
      361 gattttgccc gcacggaaga ccgccttgtc gaagtgttgg cacaacctgc cgttttcagc
      421 ctgaaactgg gtattttgct tttattgccc gccattttcc tgcgtttgaa aagaatgtga
      481 aaacaaattg gcggggcggg ggaatgccca tgcggcaatt ttacgttttg ggttatgagg
      541 ataaccgtta taatcacaac tttacagtct gcacggagaa aaccgatgcc ttggaatctc
      601 cccatcttcc tcacatggtt gagggtcttg ctcattcctg tcatgacagc ccttttttac
      661 ctgccttttc cgtggttcgc ggaggaaacg attaatctca ccgccgccgt catttttgcc
      721 gttgccgcct taaccgactg gtttgacgga tttttggcaa gactgtggaa acagacttcc
      781 gatttcggcg ccttcctcga tcccgtcgcc gacaagctga tggttgccgt atcgctactt
      841 ttactggtca aactcgaccg gacctatgtt ttgtttgcca tgattatcat cggcagggaa
      901 attaccattt ccgcattgcg cgaatggatg gcgcaaatgg gcaaaaggaa cagcgttgcc
      961 gtcgccaccg tcggtaagtt caaaaccgcc gcgcaaatgc tggcaatctt cctgctgctg
     1021 ctgaatattc ccgattttta cggatttaat cttgttgtta tcggcaatgt attgatgttt
     1081 atcgcatctt tgctgacagt ctggtcgatg ctgtattatc tgaaaatggc gtggaaagaa
     1141 atcgcctgaa aaaaacataa aaatagcttg acggcaaaaa cagaatccat aataattgcg
     1201 tctcttcgat gttggagaat gaaatcgggc gggaatagct cagttggtag agcgcaacct
     1261 tgccaaggtt gaggtcgcga gttcgagact cgtttcccgc tccaaaaatt tcattttccc
     1321 acaatcattg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1381 agttcgagac tcgtttcccg ctccaagttt tatttcaaac gtatcaacgc gggaatagct
     1441 cagttggtag agcgcaacct tgccaaggtt gaggtcgcga gttcgagact cgttttccgc
     1501 tccaaaaacc gatatgtttg aacgcgcggg aatagctcag ttggtagagc gcaaccttgc
     1561 caaggttgag gtcgcgagtt cgagactcgt ttcccgctcc aagtttttta gataagccag
     1621 ttttagggcg agatagcaaa gtggttatgc agcggattgc aaatccgtct acgccggttc
     1681 gattccgact ctcgcctcca ttatcgtact atggcggggt ggcagagtgt ttatgctatg
     1741 aggagtgcaa tcttcataca ggccggttaa aatccgcgcc cccgcctcca cctttcacaa
     1801 atgcccgggt ggtgaaatag gtagacacaa cggacttaaa atccgtcggg actaaacatc
     1861 ccgtgccggt tcgattccgg ctccgggcac caagctgaaa atgacaatgc cgctccaagc
     1921 ggttattttt ttatctgtcg gaaggtgatg gacaatattg acatgttcat gcctgaacaa
     1981 gaggaaatcc aatcaatgtg gaaagaaatt ttactgaatt acggtatttt cctgctcgaa
     2041 ctgcttaccg tgttcggcgc aattgcgctg attgtgttgg ctatcgtaca gagtaagaaa
     2101 cagtcggaaa gcggcagtgt cgtactgacg gatttttcgg aaaattataa aaaacagcgg
     2161 caatcgtttg aagcattctt tttaagcggg gaagaggcaa aacatcagga aaaagaggaa
     2221 aagaaaaagg aaaaggcgga agccaaagca gagaaaaagc gtttgaagga gggtggggag
     2281 aaatctgccg aaacgcaaaa atcccgcctt tttgtgttgg attttgacgg cgatttgtat
     2341 gcacacgccg tagaatcctt gcgtcatgag attacggcgg tgcttttgat tgccaagcct
     2401 gaagatgagg ttctgcttag attggaaagt ccgggcggcg tggttcacgg ttacggtttg
     2461 gcggcttcgc agcttaggcg tttgcgcgaa cgcaatattc cgctgaccgt cgccgtcgat
     2521 aaggtggcgg cgagcggcgg ttatatgatg gcgtgtgtgg cggataaaat tgtttccgct
     2581 ccgtttgcga ttgtcggttc ggtgggtgtg gtggcggaag taccgaatat ccaccgcctg
     2641 ttgaaaaaac atgatatcga tgtggatgtg atgacggcgg gcgaatttaa gcgcacggtt
     2701 acttttatgg gtgaaaatac ggaaaagggc aaacagaaat tccgacagga actggaggaa
     2761 acgcatcagt tgttcaagca gtttgtcagc gagaaccgcc ctcaattgga tattgaggaa
     2821 gtggcaacgg gcgagcattg gttcggtcgg caggcgttgg cgttgaactt gattgacgag
     2881 atttcgacca gtgatgattt gttgttgaaa gcgtttgaaa acaaacaggt tatcgaagtg
     2941 aaatatcagg agaagcaaag cctgattcag cgcattggtt tgcaggcgga agcttctgtt
     3001 gaaaagttgt ttgccaaact tgtcaaccgg cgggcggatg tgatgtagtt tgccgaacgg
     3061 atggaaatgc cgtctgaagc gtgttcagac ggcatttttc aagttccggc tttgatgttc
     3121 cgttccaatc cccctatccg ttgtccgatg tctttcaaat tggaatattg ttcgaccaat
     3181 cgcggcacgt cgtccaaact catggcaaac atccacagat aagtgccgac cgaatagaca
     3241 tgccccgcgc tgctgtagcc tttgagcgtc atcatcacaa aagcaaagcc gaacaaaata
     3301 cccatcgccg tgccgacgca gagatagccg aaggcttcgc ggttggaaat cagcacacgc
     3361 aggcgcgcaa ccagtccgta atggcggtac agctgccgtc ggtctccttt tcggataaag
     3421 tggttgtcgc attccaagct gttgttcagg cggaaataca ggttttcgct aatggcggca
     3481 aaacgtggca aaagccataa aaacaacgca agtatgccca ccgccgacac gccgacccaa
     3541 aattccagca ccagcagcat gatgcacgcg ccgaatatgg atacgacgga tgtcgcggca
     3601 atcggcaggt gttcttcaaa aaagctgaca aactcacgcg acagggcaac ccgcgcagtt
     3661 accgccgaat gcgggacttg tcgctgccgc tgttccaaca cgaccggcac ggcgatttcg
     3721 gtataaatcc gcgtaaacgt gcgcgtatcg gcaatccgcc gcgccgcacc gaccagccac
     3781 atcaaaagca caactaaagc gtacagcaac gcctgccaca cctcccccgc aatcacggca
     3841 ttgatcgccc ggccgccaaa caccggatac accagcatca aaaggttttc cagtccgacc
     3901 agggaaaatg tgccaatcaa tcgcttgcgg tgggtttggg ctatgtgttt caacattttc
     3961 cacatatttg cccccctatt ttttattttc cgagattgtt ttttccatca cttcagccag
     4021 tgcatccaaa tcggcaaaca gccgggttgt gcgcttgtcg ccgaatgtgg caaatacttt
     4081 gtgagacctt tgcaaaattc ctttccctcc cgacagccga aacccaaaca caggttttca
     4141 gctgttttcg ccccaaatac c
//
LOCUS       DAXHTY010000118         4144 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXHTY010000118 DAXHTY010000000
VERSION     DAXHTY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.118"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(78..623)
                     /locus_tag="ACFNMK_001843"
                     /pseudo
     CDS             complement(78..623)
                     /locus_tag="ACFNMK_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1631..2401)
                     /gene="xth"
                     /locus_tag="ACFNMK_001844"
     CDS             complement(1631..2401)
                     /gene="xth"
                     /locus_tag="ACFNMK_001844"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6365042.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMVEALKDVRVDLETRALERPSDHAPVAAEFDW"
     gene            complement(2461..2742)
                     /locus_tag="ACFNMK_001845"
                     /pseudo
     CDS             complement(2461..2742)
                     /locus_tag="ACFNMK_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
     gene            complement(3211..4092)
                     /gene="nadC"
                     /locus_tag="ACFNMK_001846"
     CDS             complement(3211..4092)
                     /gene="nadC"
                     /locus_tag="ACFNMK_001846"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC6365043.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSRENGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAKYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGSLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAASRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
BASE COUNT          993 a         1025 c         1033 g         1093 t
ORIGIN      
        1 tcgggtattt ctgacgattc atcgagccgt agcaactgta tttttcaccc cgtcgggcaa
       61 aaataccaaa actcaaatca agccgtccgg ataccgtttt cggcggtatc gttttcggcg
      121 gtatcgtttt cggcggtatc gttttcggca aaataatcac gcatccgggc attcgatatc
      181 gtcagcagtt tgcgcataca tgccgtaacg gcaaccttat acggcttacc ctcggacagc
      241 aggcgttggt agaaatcccg aataagcggt tcaaaacgtg tcgctgccac ggtagccata
      301 tacagtgcct tacgcaccgc agaccttccg ccaaagcagc ggcttttaaa tttggtttcc
      361 ccgccctccc tcgggtgcgg ggcaatgccg gccaaacccg ctatccgttt gtgcgacagc
      421 cgcctcaatt cgggcagcat cgccatcagc gtagccgtcg ttatcgaacc gatgcctttg
      481 atttgctccg ccacttgggc tttgccgtca aaatgcgtgt gggtgtggtc gtcgatttgt
      541 ttgtccaatt cgtcaatcag ccggtcaaaa tgggcaatca gttgtttgac gcttccgact
      601 ttcgtttcat gaaactgatg cagatatagt ggattaacaa aaatcaggac aaggcgacga
      661 agccgcagac agtacagata gtacggcaac gctgtactgg tttaaattta atccactata
      721 ttttgtaata gctgccatta tgcctactag gatatatacc ttctatataa tccttattta
      781 aataaaaacc cttataaaaa tctgtgttat ctgtcgggat tgaaaaaaag attttatctt
      841 tctcatcagg tttatatttg ctatcaatat caaaaagaga ataaggatta caatgagttc
      901 gacatactag gtaaaaattt atatcttgga tcatgaaata ttcgatattt atcaggatcg
      961 aaagatgcta aaaattcatc aatattggta ctggatattt tgtcatcact aataaacatc
     1021 gctagagatg ggcgactttg attttccatt tgttgattag taaaggatat aaatactaac
     1081 ttgccattgt caaaccacat agatttatat agtggattaa atttaaacca gtacgtcgtt
     1141 gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
     1201 ttaatccact atagttattg cctttgggag aatcaattct gacagcacat aacataggtt
     1261 catctttaca gtcgttatat tgggtttcat caaactcaac tgcatagcac aataaaggca
     1321 ataggaaaaa aaggagatta atttattact atacataagg cagtctgact attcattcta
     1381 aaataaatcc ggtttttaat atttgattct gctccggatt atatacataa taaattttat
     1441 tgctccaaaa tctgatatta accctatgga tttcataaat ttttcagaaa aataggctgt
     1501 gcttgaacaa tccgccgact ccggctgttt gtcagggaaa ggcaattatt tttcggtcag
     1561 gcagaaagtt atcctattga atcacttaaa gatatggatg tttgtcaacg ccatatcaaa
     1621 acacggtctt ttaccaatcg aattctgccg ccacgggcgc gtggtcgctc ggacgttcca
     1681 gcgcgcgcgt ctccaaatcg acgcggacat ccttcaacgc ctccaccatc gcaggcgaca
     1741 ccaaaatatg gtcgatacgc aggcccagtt tgcgttggaa catcgcgccg cgatagtcga
     1801 accaggtata gaacgcgcct tcgggatgga cttggcgcag gctgtcggtc agtcccaaat
     1861 ccagcaggtt ttgaaaccac tgccgttcga cggacgaaca gtggattttt tcgtgccatt
     1921 tttcagggtc gtaacagtcc gcatcggcag gcgcgatatt gaaatcgccc agcagcacca
     1981 gtttgccgta gcgggtcatt tcatcgcgga caaactccgt cagtgcggca aaccattgtt
     2041 ctttatattt gaatttcggg ctgtcgaggg cttcgccgtt gacgcaatag acattgataa
     2101 cgcgcacacc gccgacggtt gccgcaatca cgcgccgttg cggatcgtcc ggcagcgcgg
     2161 gcagcccgat atgcacgtct tccggcgcgt tgcggctgac gattgccacg ccgttgtagg
     2221 ttttctgccc gctccaaaca cagtgccagc ccatcatttg caaagcggcg gccggaaatt
     2281 tgtcctgatc gagtttgagt tcctgcaaaa ccaaaatatc gggcggattg tcggcaagca
     2341 ggttttgcac ctgcggcagc cgcacattga gcgaattgac gttccaagtg gtgattttca
     2401 taacatttcc gaaaaatgcc gtctgaaacg ttcagacggc aaaaggaaag gattttaaca
     2461 ctataccgcg cgtttgtttt ccaaatcgcg gattgtccgc agaatcgttg ccgcgtcttc
     2521 ggaaatcagg cggtattcga taatgcggtg ctaacgcgta aagtccacca gaccttccac
     2581 gcgcagtcgg ctcaaatggt tggaaaccgc tgtcgcaggc atggacaggg attcggacag
     2641 ctcggcaata ttgcaatcgc cgtccaacag ttggagcagt atcgccatac gttcgggatg
     2701 cgccataagt ttgagtatgg tgtggagcgg tattgtgttc atcggtattc ctaaaacctt
     2761 ttccgcccgc caagcggatt gacgggcggc agatatggat gattgtgatt atctttataa
     2821 attatattat aaatcaatta tttatatatg attgtacagg attgtatgtt ccttttacca
     2881 ctatttttgc aaacaagcgt aaaaggcgga tcttcagacg gcataaaact gccgccccta
     2941 ccctgcctca ccctgttgcc gaacggaatc atccgcattg ttcaaaagat gccttacaac
     3001 cgccgcacgt cttgccctgc cgatgcctcc gtgccttcgg gcaaacaaaa tacctgcctg
     3061 aaatgctgtt ttttctaaag catcacgacc ttcacgaaaa atgccgtctg aagaatattt
     3121 cagacggcat ttcctccttg aggcaataac cggattacgg ttaggcggtt ccttgtctga
     3181 catatcagac aactgccgac accctaaaac tcatgccaca aaatccaaac ctatgtccaa
     3241 agagcggctg ctgtgggtca gatagccgag ggcgatgccg tccacgccgg tttgtgccac
     3301 gcgcttcagg cggtcgaagc cgatgccgcc cgatgcttcg caatagacgg tgtgggggtg
     3361 ggcggtttgc gtgtggcagc ggcttgccgc ttctttcagg gtttcgtcgt ccatgttgtc
     3421 cagcaaaatc cgttccgcgc ccgctgcgat ggcttcgtcc agttgtgcca gcgtgtccac
     3481 ttcgatttcc acgcaggtca acgatccgac cgcctgtttt gcctgccgaa ctgcttgggc
     3541 gatgctgccg caataggcga ggtggttgtc tttgatgagc acggcgtcgt ccaagcccat
     3601 gcggtggttc acgccgccgc ctgccctgac ggcgtatttt tgcaggacgc gcagcagggg
     3661 gatggttttg cggctgcaca cgatgtctgt accgtatttg gcgacttcgg caacggcacg
     3721 cgcggtggcg gtggcgatgc cgcttaagtg cgtgaggtag ttgagcgcgg tgcgttcggc
     3781 ggcgagcagc gcgcaggcgt tgccttcgac ggcagccagc gtctgacctg cgcggacgac
     3841 ttgcccgtct cggatttcgg cttggaagcg aacggacgga tccatcgtct gaaaggcgag
     3901 gcgcgccaag tccatgccgg cgataacgcc gttttcgcgg ctgacgagga aaagtttggc
     3961 ggttttgtcg ggggcgataa cggcggcgga cgtaatatcg ccgcgcctgc ccaaatcttc
     4021 gctcaaggct tgttccacca tggggcgcaa cagggtgtcg ggcagtggga agagggtgtt
     4081 ttcagacggc atggggtttc ctttgttgaa aaagattgat gttgcaccga tggggcaaaa
     4141 cttc
//
LOCUS       DAXHTY010000119         4141 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXHTY010000119 DAXHTY010000000
VERSION     DAXHTY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.119"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            142..2022
                     /locus_tag="ACFNMK_001847"
     CDS             142..2022
                     /locus_tag="ACFNMK_001847"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HFC6365044.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLALPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAVKPGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDGE
                     FNVGAPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAITL
                     QKARGKYEPNRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITGLDPEKQFVSLNYIKAVAPAGTTAGN
                     AV"
     gene            2079..2468
                     /locus_tag="ACFNMK_001848"
     CDS             2079..2468
                     /locus_tag="ACFNMK_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365045.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YPYLDNCLVTLTVDRISVSFIREIQAA"
     gene            2477..2860
                     /locus_tag="ACFNMK_001849"
                     /pseudo
     CDS             2477..2860
                     /locus_tag="ACFNMK_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233580.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
     gene            complement(3184..>4141)
                     /locus_tag="ACFNMK_001850"
     CDS             complement(3184..>4141)
                     /locus_tag="ACFNMK_001850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6365046.1"
                     /translation="AKVAKVAKVAKVAKVAKVAKVAATAQKEAAPAQQQGGIRFSDGI
                     AAVADNEVITRRRLAEAVAEAKANLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRN
                     IQASEAEIDAVVAKNPALKNLSPAQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFL
                     EQAQKQGITLPEGAPLRQYRAQHILIKADSENAAVGAESTIRKIYGEARSGTDFSSLA
                     RQYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVR
                     EAGTPQERIRNSVRQYIFQQKAEQATVNLLRDLHSGAYVDIR"
BASE COUNT         1089 a         1080 c         1005 g          967 t
ORIGIN      
        1 aacgctgtac tggtttttgt taatccacta taacgcaagc accgcaaaga cgcgccaacc
       61 ctctcccaac cttttttcag acggcatttt cggtaatctg ctaaaatcgc ccgcttgagt
      121 ttccacagaa aaatccgaaa aatgaatatt ttttacgaag agtccggcca attcaaaatc
      181 gccgccatca tccaaaaaaa cgatgccacc taccaagtcg ataccccaca cggcaaacgc
      241 accaaagtga aggcgaacaa cgtctttgcc gagtttgacg gcgatatggc ggcgtttttg
      301 gaaaacgcgc aggcacaggc ggcggacatc gacaccgatt tattgtggga agtatgcggc
      361 gaagaggaat ttaccgccga agccatcgcc gaagaatatt acggccatgc gccgaccaaa
      421 accgagctgg cggcaacttt gattgcgctt tacgccgcgc cgatgtattt ctacaaaaaa
      481 gccaaaggcg tgttcaaagc cgcgcccgaa gaaaccttaa aacaagcact tgccgccatc
      541 gaacgcaaaa aacagcaaga cgcgcaaatc gacgcttggg cggaagcctt gaaacgcggc
      601 gagatgccgt ctgaaatcgc ggcggatttg aaaaccatcc tgcacgcgcc cgacaagcag
      661 tcgctgacct acaaagcctt taccaaagcc gccgacgcgc tgaaaacctc tgcctacgaa
      721 ttggcgaaaa aaacgggcgg cattacgtcc attccccaat acctgcaaga cgggtttgaa
      781 atcaaatact tccctaaagg aacaggcttc cccgaccttg cccttcccga aatgcccgac
      841 ctgcccaagg ccgacgttac cgccttttcc attgacgacg aatcgaccac cgaagtggac
      901 gacgctttaa gcctgaccga cttggacaac ggcacgaagc gtgtcggcat ccacatcgcc
      961 gcgccgtcac ttgccgttaa accgggcgac aaaatggaaa aaaacatcat ggaacgcttg
     1021 agcacggttt atttccccgg cggcaaaatc acgatgctgc ccgaaaactg gattgccgcg
     1081 ttcagccttg atgcaggcgc acaccgccct gccgtcagca tttatttcga tgtggacggc
     1141 gagttcaacg tcggcgcgcc gacctgcaaa atcgaagcgg tcaacatctc cgaaaacctg
     1201 cgtatccaag ccatcgaacc gcatttcaac gccgaagcgg gcttggacga agcgggcgaa
     1261 atgatgttcg cccaccatca agacctgatt tggttttatc aattcgccat caccctgcaa
     1321 aaagcgcgcg gcaaatacga gcccaaccgt gcgccgcaat acgattacag catcgaattg
     1381 gataaagaag acaacgtatc cgtcgtccgc cgcgaacgcg gctcacccat cgatacgctg
     1441 gtcagcgaga tgatgattct tgccaacagc acttgggcac aaatgctcca tgacaacgac
     1501 ctgcccggcc tcttccgcgt ccaacccacc ggaaaagtac gcatgagcac caaatccgag
     1561 ccgcatatcg gcatgggcgt gcagcattac ggctggttta cctcgccgct gcgccgcgcc
     1621 gccgactaca tcaaccaaaa gcagctgatc agcctgatag acgacactgc cgagccgctg
     1681 tatcaaaaca gcgatgccga gcttttcgcc gcactgcgcg actttgatgc cgcctatacc
     1741 gcctacgccg atttccaacg gcagatggaa gcctactgga gccttgtgta cctgcaacag
     1801 caaggtacaa gcgagctgac cgcgaccatc ctcaaagaag acctcgtccg catcgaaggc
     1861 ctgccgctgg tcacgcgcgc aaccggtatt ccgtttgacg cgctgcccaa atcgcaggca
     1921 ttgtttaaaa ttaccggact cgacccggaa aagcagtttg tctcgctcaa ctacatcaag
     1981 gcagtcgcac ccgcgggtac aacggcaggc aatgccgtct gaagcccgat acggcaaact
     2041 ccggcaacaa aaaacggacg gcaaaaaggc aagataaaat gttatataaa tgtaaaatca
     2101 aaaagttaga taacaccatt gaagaggcag tattgattga aataaatggt tatgaactac
     2161 attgttttat atcatactgc ccattcaaaa tatttgaaaa tcaattttat gaagtggaat
     2221 tatcctatga aatctttaat gattacatac tggataaatc aacttcaaat attggattta
     2281 gaaaaataga tgatagtttc aagtatgaaa taacggcaga attaaaaaat aatcatctaa
     2341 ccctacaaaa tatacaattt gaagaagatg agtatttaat aaactaccct tatcttgata
     2401 attgtttggt tacattaacg gttgatagaa taagtgtctc atttattaga gaaattcagg
     2461 cagcctgaaa cctgttgtgg ataccgttgg cagtttagcg attggttatg ccacaggcgg
     2521 caatgctggg gcttttactg gtgcgaatgc agattggaat aatcggcagt tgcattcgag
     2581 tgaatataaa ttagcagaac aatatgcaga catagtagct tctcgtctaa acatttcaaa
     2641 agaagaagca cttgggcgaa ttattcggca tatgcaacga accgtagatt acgcaacggc
     2701 taaagcggat aatttccgta ctgatgacgc cattatttca attttaggag caaacaattt
     2761 acccaagcaa gatcaacatt acactaatcc gaactacaac aaacaataca ttcaaaatta
     2821 tgttcgtgat ttcaatttag ccaataaata caattatttt atgccgtccg tttttacttt
     2881 tatcccgatt tgcggggcaa gttttccaac tgcatcctgt caaaaaattc cattattttc
     2941 aaatacttaa aagttaaaaa ggttaaattt ttcttaacaa tcaaaaacat tgtttaacgg
     3001 aatattaact taaagagcag ctagaaaacg agcttttcgc cgtccaccgg aaaactttta
     3061 cggcgtttgt ttattttttt caatatgttc caccgcttcg tccgcctgtc tgccgccgca
     3121 gtttgaaaaa gccgtgccgc ccgaaggttg tcgggacggc acggcttttc agtcacgacc
     3181 gtttcagcgg atgtccacgt aggcaccgga atgcaggtca cgcaacaggt tgacggttgc
     3241 ctgttcggct ttttgttgga agatgtattg ccgcacggaa ttgcggatac gttcctgagg
     3301 tgtgccggcc tcgcgcactt cgttcaattt gatgatatgc cagccgaatt gggtgcggac
     3361 gggcgcgccg acctgtccgg gtttgagcgc gtggacggct tcttcaaagg cgggaaccat
     3421 cacgccgtcg gcaaaccagc ccaaatctcc gccgttgccc gcgctcgcgt cttgcgaata
     3481 ttggcgcgct aggctggaaa agtctgtgcc gctgcgggcc tctccgtaga ttttgcggat
     3541 ggtgctttcc gcgccgacgg cggcgttttc gctgtcggct ttaatcagga tgtgttgggc
     3601 gcggtattgg cgcaacggtg cgccttcggg cagggtgatg ccttgttttt gcgcctgctc
     3661 gaggaaggca tcgatttcag cttcgctcac gcggctgttc tgcatcactg cctgctggcg
     3721 gactttttcg gcaatgatgt tgtcggcaaa atcgcggcgt tgggcggggc tgaggttttt
     3781 gagggcggga ttttttgcga cgacggcatc gatttccgct tcgcttgctt gaatgttgcg
     3841 gcgtttgccc gcctgtacaa tcagggattg gttgacaagc tgcatcagca cctgtcggga
     3901 cagctcggat tcgcttatct gcgcgtcttt gggcaggttg gctttggctt cggcaacggc
     3961 ttcggcaagc cggcggcgcg tgatgacttc gttgtcggca acggcggcaa tgccgtctga
     4021 aaagcggata ccgccctgct gttgtgcggg tgcggcttct ttttgcgccg tggcggcaac
     4081 tttggcaact ttggcaactt tggcaacttt ggcaactttg gcaactttgg caactttggc
     4141 a
//
LOCUS       DAXHTY010000120         4129 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXHTY010000120 DAXHTY010000000
VERSION     DAXHTY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.120"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            43..675
                     /locus_tag="ACFNMK_001851"
     CDS             43..675
                     /locus_tag="ACFNMK_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC6365047.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="ACFNMK_001852"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="ACFNMK_001852"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC6365048.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKER
                     FSGVPEVAAASEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTGMAHEL
                     AAVPMMSRTHGQPATPSTLGKEIANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQAISRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKDGITPEVLKLFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2197..4065
                     /locus_tag="ACFNMK_001853"
     CDS             2197..4065
                     /locus_tag="ACFNMK_001853"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6365049.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNDRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFWGFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSRFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTASGKRQLLSLLCFGALLACLFVIDKHNPFILGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDTTLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRGGALQ"
BASE COUNT          938 a         1105 c         1070 g         1016 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcggtc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 acggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttccgaa tacggcctga
      841 tgaaagcgcg cgtcaaagtc gaattaaact ggctcaaagc cctcgccgcc gagccgaaga
      901 ttgccgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccacgacc aatcatgatg
     1021 tcaaagccat tgaatattgg cttaaagaac gcttctccgg cgtgcctgaa gtggcagctg
     1081 ccagcgagtt catccacttt gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa actggccgaa atcatcgaaa
     1201 aactgaccgg catggcgcat gagttggctg ccgtcccgat gatgagccgc acccacggcc
     1261 agcctgctac accaagcaca ctgggtaaag aaatcgccaa tgtcgtgtac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gcgcaagagt tcctcggcaa aatcaatggc gcagtcggca
     1381 actacaatgc ccatatggtc gcttatcccg atgtggattg ggaaacccat tgccgcaact
     1441 tcgttgaaat cagcctcggc ctgaccttca acccctacac cattcaaatc gagccgcacg
     1501 actatatggc ggaattcttc caagccatca gccgtgtcaa cacgattctg atcgacttca
     1561 accgcgatgt ttggggctat atttctttgg gttacttcaa acaaaaagtc aaagcaggcg
     1621 aagtcggttc ttccaccatg ccgcacaaag tcaacccgat cgactttgaa aactccgaag
     1681 gcaacctcgg tatggcaaac gccgtattgg gcttcttatc cgaaaaactg ccgatttccc
     1741 gttggcagcg cgacctgacc gacagcaccg tattgcgcaa tatgggcgta ggcgtgggct
     1801 atgccgtgtt gggtttcgcc gcccatctgc gcggtctgaa caagctcgaa cccaaccctg
     1861 ccgcgcttgc cgccgatttg gacgctactt gggagctgct cgccgagccg attcaaaccg
     1921 taatgcgtcg ttacggcgta gccaatcctt atgagaagct gaaagacctg acgcgcggca
     1981 aagacggcat tacgcctgaa gtgttgaaac tctttatcga gtcgttggaa attccggcag
     2041 aagccaaagc caaattgctt gagctgaccc ccgcactgta tgtgggtaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacggggcg atatttatgc aagctgtccg atacaggcct
     2221 gaaattgacg gattgcgggc cgttgccgtg ctctccgtca ttattttcca cctgaatgac
     2281 cgctggctgc ccggagggtt tttgggggtg gacattttct ttgtcatctc gggattcctc
     2341 attaccggca tcattctttc tgaaatacag aacggttctt tttctttctg gggtttttat
     2401 acccgcagga ttaagcggat ttaccctgcc tttatcgcgg ccgtgtcgct ggcttcggtg
     2461 attgcctctc aaatcttcct ttacgaagat ttcaaccaaa tgcggaaaac cgtggagctt
     2521 tctgcggttt tcttgtccaa tatttatctg gggtttcagc aggggtattt cgatttgagt
     2581 gccgacgaga accccgtact gcatatctgg tctttggcgg tagaggaaca gtattacctc
     2641 ctgtatcctc ttttgctgat attttgctgc aaaaaaacca aatcgctacg ggtgctgcgt
     2701 aacatcagca tcatcctatt tctgattttg actgccacat cgtttttgcc aagcaggttt
     2761 tataccgaca tcctcaacca acccaatact tattaccttt cgacactgag gtttcccgag
     2821 ctgttggcag gttcgctgct ggcggtttac gggcaaacgc aaaacggcag acggcaaaca
     2881 gcaagcggaa aacggcagtt gctttcatta ctctgcttcg gcgcattgct tgcctgcctg
     2941 ttcgtgattg acaaacacaa tccgtttatc ctgggaatga ccctgctcct tccctgcctg
     3001 ctgacggcac tgcttatccg gagtatgcaa tacgggacac ttccgacccg catcctgtcg
     3061 gcaagcccca tcgtatttgt cggcaaaatc tcttattccc tatacctgta ccattggatt
     3121 tttattgcct tcgcccatta cattacaggc gacaaacagc tcggactgcc tgccgtatcg
     3181 gcggttgccg cattgacggc cggattttcc ctgttgagct attatttgat tgaacagccg
     3241 cttagaaaac ggaagatgac cttcaaaaag gcatttttct gcctctatct cgccccgtcc
     3301 ctgatacttg tcggttacaa cctgtacgca agggggatat tgaaacagga acacctccgc
     3361 ccgttgcccg gcgcgcccct tgctgcggaa aatcattttc cggaaaccgt cctgaccctc
     3421 ggcgactcgc acgccggaca cctgcggggt tttctggatt atgtcggcag ccgggaaggg
     3481 tggaaagcca aaatcctgtc cctcgattcg gagtgtttgg tttgggtgga tacgacactg
     3541 gcagacaacc cgttatgtcg aaaataccgg gatgaagttg aaaaagccga agccgttttc
     3601 attgcccaat tctatgattt gaggatgggc ggccagcccg tgccgagatt tgaagcgcaa
     3661 tccttcctaa tacccgggtt ccaagcccga ttcagggaaa ccgtcaaaag gatagccgcc
     3721 gtcaaacccg tctatgtttt tgcaaacaac acatcaatca gccgttcgcc cctgagggag
     3781 gaaaaattga aaagatttgc cgcaaaccaa tatctccgcc ccattcaggc tatgggcgac
     3841 atcggcaaga gcaatcaggc ggtctttgat ttggttaaag atatccccaa tgtgcattgg
     3901 gtggacgcac agaaatacct gcctaaaaac acggtcgaaa tacacggacg ctatctttac
     3961 ggcgaccaag accacctgac ctatttcggt tcttattata tggggcggga atttcacaaa
     4021 cacgaacgcc tgcttaaatc ttcccgcggc ggcgcattgc agtagcctgc cttcttgtcg
     4081 gatattgcct ttggcagcct atgccgctgt ttgcccttcg gggcggcgg
//
LOCUS       DAXHTY010000121         4093 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXHTY010000121 DAXHTY010000000
VERSION     DAXHTY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.121"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            441..2222
                     /locus_tag="ACFNMK_001854"
     CDS             441..2222
                     /locus_tag="ACFNMK_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC6365050.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHAVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVKLYQRLRQPVPYAV
                     LSHLPDAQGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENGRCALLDRHLCRLKTSAQALNLPLPDGCENQIKQYIARLPDGAFRIKALLASDG
                     ISLSRAVLNRLTDKQRVIISPTILPAQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
     gene            complement(2281..3798)
                     /locus_tag="ACFNMK_001855"
     CDS             complement(2281..3798)
                     /locus_tag="ACFNMK_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6365051.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
BASE COUNT          982 a         1321 c          926 g          864 t
ORIGIN      
        1 tagattcccg cctgcgcggg aatgacggct acagatgccc gacggtcttt atagcggatt
       61 aacaaaaatc aggacaaggc gacgaagacg cagacagtac agatagtacg gaaccgattc
      121 acttggtgct tcagcacctt agagaatcgt tctctttgag ccaaggcgag gcaacgctgt
      181 actggttttt gttaatccac tatacttgga gctgatcttg cttttcgcct aattctacgt
      241 tttcaaacgg ttgcagctgg tggtctgtca taaaggtctc cttattgtat ttcaggttgg
      301 aaatcggaat ttgttttcac aattttacac cttcgccccc cactttctct acataaaatt
      361 acattttgcc gatatttgcc gaatcgtctg aaaatatgtg taaaaagagc gtataatcaa
      421 aacatttgcc ccgaattgcc atgccttatt tcgccctgtt tgacgatgcc gtaagcggcc
      481 gcgcaaaacg ctatcaaaat catgtggaaa gccgtttttt ccgtcccgaa gaactcgatg
      541 ctttggacgg cgcgctgcaa tcgggctggc aaaaagggct gcatgccgtg ttgtttgcag
      601 actacggatt cggtttgccg ctgacggggg tcgagtccga acgcggcggc aaccttgccc
      661 tgcactggtt tgccgactgc gccgacaccg atgccgcaag ctggcttgcc cgacactcag
      721 acggcctccc cgccggcatt tccacgccgc aatcctccgt atccgaagcc gattacctcg
      781 accatatccg ccaaatccac gaagccatcc gacgcggcga cacctatcaa atcaactaca
      841 ctacccgcct gcacctgcaa gcctacggca atcccgtcaa actttaccaa cgcctgcgcc
      901 agcccgtccc ctatgccgtt ttgtcccacc tgcccgatgc acaagggcaa tccgcgtgga
      961 cgctgtgttt ctcgcccgaa ctcttcctca acatcgcttc ggacggcacc atcagcaccg
     1021 aaccgatgaa aggcaccgcg ccgattttgg gcgacggaca agacgaacgc cgcgccgccg
     1081 agttgcaagc agacccgaaa aaccgcgccg aaaacgtgat gattgtcgat ttgctgcgta
     1141 acgacctcgg caaaatcgcc caaaccggca cagtatgcgt acccgaaccg tttaaagtgt
     1201 cgcgtttcgg cagcgtttgg cagatgacca gcaccatcca agcccaagcc ttgccgcaca
     1261 cctcgttcgc cgacatcctc cgcgccgcct tcccctgcgg cagcatcacc ggcgcgccca
     1321 aaaaaatgag tatgcagatt atcgaatcgc tcgaagccga agcgcgcgga ctttatacgg
     1381 gcagcatcgg ctatttgaac ccgtgttccg gcggcttggg gtttgaaggc acgttcaacg
     1441 tcgttatccg caccttgtcg ctcacgccgc tttcagacgg catttatcaa ggcgtgtacg
     1501 gtgtcggttc cggcatcgtc atcgacagcg accctgccgc cgaatatcgg gaatgcggct
     1561 ggaaagcccg tttcctcaac gaattgcgcc ccgacttcgg catttttgaa accctgcgcg
     1621 tggaaaacgg acgctgcgcc ctgctcgacc gccacctatg ccgtctgaaa acctccgccc
     1681 aagccctcaa cctgcccctg cccgacggtt gcgaaaatca aatcaaacaa tacatcgcac
     1741 gcttgcccga cggcgcgttc cgcatcaaag ccctgctcgc ttcagacggc atcagcctgt
     1801 cccgcgccgt tttaaaccgt ctgaccgaca aacaacgcgt catcatttcg ccgaccatcc
     1861 tgcccgcaca aaactacctg cgccgcttca aaaccacctg ccgcaccgtc ttcgaccaag
     1921 cgtggcaaac cgccgaaaca caaggcgcgt tcgacagcct gtttttcaat tcagacggca
     1981 tcctgctcga aggcggcaga agcaacgtgt tcgtcaaaca tcgcggacaa tggcttacgc
     2041 cctctttaga tttggacatt ttaaacggca taatgcgcca agccgtattg gacgaaccgc
     2101 aaaaatattt gcaaacaaat caagtaatcg aaacacacat cacacaaaaa acactgcaag
     2161 aagccgaaga aatccgcctc tccaacgcct tgcgcggcgt atttgccgcc gcccttgcct
     2221 gaatatgcaa aaatgccgtc cgaacctgtt tccaaagttc ggacggcatt atcccaccat
     2281 tcaaaaccgc caatccgccg acacaaacac ctcgctgttg cggcgtttcg catacggcac
     2341 attactttcc gtcctgccga aacgataatt caacgccggc acgatacctt tgtacgacaa
     2401 cttgtcgtgg ctcaaagcca gcgagacatt ccattcgcgg ttgcgttgcg cctctgtcga
     2461 gaaagccgca atgcccttat agttgcggcg ggcataagac gcggaaaccc gactgttcaa
     2521 accgcccaac tgccgccact cctgcgccca gccggcataa acaccgttgc gccggtaggc
     2581 ggcattattg accgcgccgc ccaccgtttc gcgtttcggc acaaaccgca caaactgcca
     2641 gccgccgaac acagttgccg attcgcccaa acgttttgcc gacgaaacat aaaacccgtc
     2701 ctgcctgccg ttattgtatt ccgccctatc ctgttcgcgg tagcgttggc ggtaatgttc
     2761 cagcgcgacc gaaaattgcc atcccgggtt tgggcggtaa gtatgggaca gctgcacgcc
     2821 gactccgtgc gccagcatat acggcggcag gcggcggttg tttacccgtt ttgttttcgc
     2881 atcaaagccg tcgctgcccg acaactgcac ctgataaaac ggcaaaatcc ccgccgtctg
     2941 ccgtgcattt ttatactgcc aacccaaata cgccctgccg aacccgtcat cataagctga
     3001 ttttttactg aaataatagc tcgtgccgcc gatattggaa cggaacaaca aataatgatt
     3061 gtccgccaac gccgtcagct tttccgcctc gatttcataa ttcaagcctg ccgcccgctc
     3121 cgcccggctg acactgcata tctgccggcc tccgttttgc cggcaatact gcggcgcggc
     3181 attattggca tttctattga ccgccgggct gatgccgccc gaaaaacgcc agcccgtcag
     3241 cccctccgtt tttttccgaa aacgccccac attttccaaa accggtgccg gcaaatccaa
     3301 ttttgccgcc tccgcaaaat gcctttctgc cgacttcagt cggaaatcgt caaactccgc
     3361 cgccgccaaa tccagcaaaa tccgctcgtc tgccgcattt tccccgtgca gttcccgata
     3421 ccgcgccacc gcctccgccg gccttcccgc caatttcgcc agcaaagccc gcgccctgcc
     3481 gtacaaaacc gcgtcataat ccggcagctt ggcatacaaa tccgccaacg aagcgattaa
     3541 atccgcctga ttgccgttga gcgcgtcgcg caaactatgt tccaacattt tcggatgtgc
     3601 caacaaaaaa tccccgtcaa ccacgcgcgg ggcatcattt tcaactttcc aatctgattc
     3661 cgcccactta tccgacaccg accgctgcac ctgcaacaat gccttgtcat ccaaaatcgc
     3721 gggcgcatcc gccccatagg cggcagaaac acctgccgca caccaaacaa ccaaaaagcc
     3781 gtatctgaaa tacaacatac cctgtcattt acctttccgg caaacacgcc gccgaagcac
     3841 gtcaaaccat ccgaaaaaca ggcaaaagcc cgtaaaaacc ggctttgccg cctgaaagcg
     3901 ggcaggcaaa aaaaccgccg cccgattttc aaagggcgga tttcacattt atagtggatt
     3961 aacaaaaatc aggacaagac gacgaagccg cagacagtac aaataggacg gaaccgattc
     4021 acttggtgct tgagtacctt aaagaatcgt tctctttgag ctgaggcgag gcaacgccgt
     4081 actggttttt gtt
//
LOCUS       DAXHTY010000122         4071 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXHTY010000122 DAXHTY010000000
VERSION     DAXHTY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.122"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..176
                     /locus_tag="ACFNMK_001856"
     CDS             <1..176
                     /locus_tag="ACFNMK_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6365052.1"
                     /translation="DFFEGKGVDVLYMHFHKANEFLGMTRLPTFLCNDVVKNPQVEKY
                     LADYQAHLEKVFG"
     gene            complement(663..1703)
                     /gene="queA"
                     /locus_tag="ACFNMK_001857"
     CDS             complement(663..1703)
                     /gene="queA"
                     /locus_tag="ACFNMK_001857"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC6365053.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLGNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGVETAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRSLESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
     gene            1820..2077
                     /locus_tag="ACFNMK_001858"
     CDS             1820..2077
                     /locus_tag="ACFNMK_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC6365054.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            2059..2514
                     /gene="accB"
                     /locus_tag="ACFNMK_001859"
     CDS             2059..2514
                     /gene="accB"
                     /locus_tag="ACFNMK_001859"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC6365055.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPVVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            2626..3987
                     /gene="accC"
                     /locus_tag="ACFNMK_001860"
     CDS             2626..3987
                     /gene="accC"
                     /locus_tag="ACFNMK_001860"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC6365056.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
BASE COUNT          997 a         1221 c          996 g          857 t
ORIGIN      
        1 gcgatttctt tgaaggcaaa ggcgttgatg ttttgtatat gcacttccac aaagccaacg
       61 agtttttggg tatgacccgc ctgccgacat tcttatgtaa cgatgtggtt aaaaatccac
      121 aagtggaaaa atacttggca gattatcagg cacacttgga aaaagtgttc ggctaaaaat
      181 ttatcttata aacaaacaaa ggcagcctga aagattgaat ggtctgcacc cctaaggttg
      241 gactaaccaa ccgactaagg tgcagattat ttagggcaga atcttcgaag gctctggcgg
      301 tgggtatcgc caaataggaa accgtccgat tgctcgggcg gttttggtgg attattgact
      361 taaaagcaac aatcaaggct agaatcattc cagcatcaat cgttacagtc aattgcaaat
      421 tgcctgctga aatagagatt tgcattttga tgactcctgt aaagtttcaa acttgtcagg
      481 aatgcaaaaa tccccttgtt ggcagcatgg ggatttttgc ttatcccacc gctctttgag
      541 tttctcgcct ctcgcgcctg aatctatgtc atacagttcc tttcattcaa atcaacaaaa
      601 gaatgccgtc cgaacgtccg ttgagacggc acttgtcttc ccccaataga cttgaggctg
      661 ttctaacgta ccaccccttc gttccgcccc aaaaccatcg catcgccgta gctgaagaaa
      721 cggtattcgc gttcgaccgc ataacgatac gcggcgcgga tatgacccat acccgaaaac
      781 gcgctgacaa gcatcagcag cgtcgatttc ggcagatgga aattggtaac cagcctgtcg
      841 gcaacattaa aacggtagcc cggcgtgatg aaaatatcgg tgtcgccctg ccccgctttc
      901 agacgacccg tcgcacgcgc ggcagactcg agggagcgca tggaagtcgt gccgaccgcc
      961 cacaccttat tgccgcgcgc tttggcggct tcaacggcgg cggcggtttc agacggcact
     1021 tcaaaccatt cgctgtgcat tttgtgttct tcgatttttt ccacacgcac gggctggaat
     1081 gtccccgcac cgacgtgcaa ggttacttct gcggtttcta cgcctttgtc tttcaggcgg
     1141 cgcaaaagtt cttccgtaaa atgcagaccc gccgtcggcg cggcgaccgc accctgatat
     1201 ttggcataaa cggtttgata acggctgtcg tcatccgcat cagcggcacg ttcgatataa
     1261 ggcggcaggg gcaggtgtcc gttctgttcc aaaagttcgt aaacggtttg accaccttca
     1321 aaacgcaggc agaacagttc gccctcacgc ccgaccatca cggcgcggat gccgccttca
     1381 aacaccagcc ccataccggg cttgggggac ttggacgaac ggatgtgcgc cagtgcggta
     1441 tggttgccca acacgcgctc aatcagggct tcgatcctgc cgccgctgtc tttctgccca
     1501 aacaaccgcg ccttcatgac tttggtgttg ttgaacacca aaacgtcgcc tgcctcgaca
     1561 taatcgggca aatcgccgaa tacccggtct tgcagcggca tatcgggcaa cgctaccaaa
     1621 agccgactgc tgccgcgcac ttcgggcgga tgctgggcaa tcagcttttc gggcagggta
     1681 aaatcaaaat ctgaaatatc catttttaca ctctcgttcg ggcaagccgc cattatacgc
     1741 actttagccc tttttcagac ggcatctttg tccgaaaaac caacagatta gaataaacac
     1801 tcttaacctg gaacatcttg tgcgcaaaat caaacttcct gcacatttcc cccaaaaacc
     1861 gccgtttttt gatattttac tggacattta ccgacaactt cgggaaaata aacacattct
     1921 cacggtcgtt ttccaccaca ggaaaaccgt atccgaacac cattccgccc ggtttgcgcc
     1981 gttgccgcaa gccggctgtt ttctgaaaaa ccaacgcaac aacccgccgg aacatcggca
     2041 gcctttaaag gaacagaaat ggatttgcgc aaattaaaaa aactgattga tttggttgaa
     2101 gaatcgggca tcgccgaaat cgaagtaacc gaaggcgagg aaaaagtccg catcacccga
     2161 accatcgccg ccgcacccgt ttacgccgcg cccgtacctg ccgccgcgcc ggtcgtaacg
     2221 cctgccgccg cacccgttgc ggcatccgcg cccgccgccg cacctgccgc ccgcgatttg
     2281 tccgacgcgc aaaaatcgcc tatggtcggc acgttctacc gcgcacccgg cccgaatgcc
     2341 gcgccttttg tcgaagtcgg ccaacaagtt aaagccggcg acacgctgtg catcatcgaa
     2401 gcgatgaagc tgatgaacga aatcgaagcc gaaaaatccg gcacggtcaa agaaattttg
     2461 gtcgaaaacg gtacgcccgt cgaattcggc gaaccgctct tcatcatcgg ataatcctgt
     2521 tttcagacgg cataaacttc ctatgccgtc tgaaatgctt tcccccttca gcgttcccgc
     2581 accctttttt acggacgggt tgccggaacc acaggaacag tcatcatgct gaaaaaagtt
     2641 ttaatcgcca accgaggcga aatcgcatta cgcgtactcc gtgcctgccg cgaaatgggc
     2701 attgccaccg tcgccgtgca ttccgaggcc gacaaagaca gcctgcacgt caaactcgcc
     2761 gacgaatccg tgtgcatcgg ccctgccgct tccgcgcaaa gctacctcaa cgtccccgcc
     2821 attatcgccg ccgccgaagt aagctgcgcg gacgctgtcc atccgggcta cggtttcctt
     2881 gccgaaaacg ccgatttcgc cgaacaggtc gagcagtccg gctttacctt tatcggcccg
     2941 aaacccgaca ccatccgcct gatgggcgac aaagtctccg ccaaacacgc gatgatagcg
     3001 gcaggcgtac cctgcgtccc cggttctgac ggcgcattgc ccgacgacgg cgaagaaatc
     3061 ctcaaaatcg ccgataaagt cggttatccc gtcattatca aagcctctgg cggcggcggc
     3121 ggtcgcggta tgcgcgtggt cgagaaaaaa gaagacctcc tccaatctgt cgaaatgacc
     3181 aaggccgaag cgggcgcggc attcggcaac ccgatggttt acatggaacg ctatttgcaa
     3241 cgtccgcgcc acgtcgaaat ccaagtgatt gccgacgaac acggcaacgc catctacctt
     3301 gccgagcgcg actgttcgct gcaacgccgc caccaaaaag tcatcgagga agcaccggct
     3361 ccgttcatca ctgaaaaaga acgcgccaaa atcggcaacg cctgtgccga cgcctgcaaa
     3421 cgcatcggct accggggcgc gggtacgttt gagtttttat acgaagacgg cgaatttttc
     3481 tttatcgaga tgaacacgcg cgttcaggtc gagcatccgg ttaccgagct catcaccggc
     3541 gtggacatcg tgcaggagca actccgcatc gccgccggcc tgcctttgca atacaaacaa
     3601 aaggatattc aagtcgaagg ccacgcgttt gagtgccgta tcaacgccga agacccatac
     3661 aacttcatcc ccagcccggg cccgattgaa agctgccacc tgcccggcgg cttcggtatc
     3721 cgcgtggaca gccacattta ccaaggctac cgcatcccac cgtactacga cagcctgatc
     3781 ggcaaaatct gcgtacacgg caaaacgcgt gaacaggcaa tggcgaaaat gcgcgtcgca
     3841 ctcgccgagc tggcggtaac cggcatcaaa accaatacgc cgcttcaccg cgacctgttc
     3901 gccgatgcgg gtttccaaaa aggcggtgtc agcatccact atttggaaca ctggctggaa
     3961 gatcgcaaag ccaaacagga caagtaaacc gccgccgata tgccgtctga agccgcccgt
     4021 ccgcgttcag acggcatttc ccttgccccg cgccgtctga aaccgatttc g
//
LOCUS       DAXHTY010000123         3988 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXHTY010000123 DAXHTY010000000
VERSION     DAXHTY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.123"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="ACFNMK_001861"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="ACFNMK_001861"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC6365057.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1160..2290
                     /locus_tag="ACFNMK_001862"
     CDS             1160..2290
                     /locus_tag="ACFNMK_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC6365058.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTVEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2361..3212
                     /gene="lgt"
                     /locus_tag="ACFNMK_001863"
     CDS             2361..3212
                     /gene="lgt"
                     /locus_tag="ACFNMK_001863"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC6365059.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3295..3957)
                     /locus_tag="ACFNMK_001864"
     CDS             complement(3295..3957)
                     /locus_tag="ACFNMK_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC6365060.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEEFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          866 a         1178 c          984 g          960 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccgg acaaacgggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaccctgcc
      181 gtcgatgatg tgcgtggctt tcacgccgtt gacggcggct tcgaccgcgg aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 gagtttttca gcgttcaatt cttccgccaa tttgcctgct accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccgcgttc
      481 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 cacgccattc tgttcgggcg tatcgatcaa aagtttcttc gccttaatga aatgatcgtc
      601 gcgtccgctt acgccgaccg cgtgcccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacgtgc ccgcccaaca ccatttcgac aatatccatc gtctctttgt cggtcacgcg
      721 cattccttgg acaaactcgc cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgccg tgaacgataa cgggatggat gccgaccagc ttcagcagca cgacatcgcg
      841 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtact tgatgacggc
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgctttgtc
      961 ggcggcggaa ataatgtttt cagactccat aacgactccc tattgttgac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatctggca gaccttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1201 cgtcaaagac ctgaccgacc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1261 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1321 gcattttgaa aacgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1381 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1441 gaccgtcaat catacgccgc tgtccaacag cgtgtattac gcctatttcg aaccttactc
     1501 cgaagaacag catttaaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1561 cgacttgggc agcaccgtgc aaggccgcga catcaatctg ctgaccatcg gcaatcaggt
     1621 cgaaagcgat ctgaaaatct ggattaccgc ccgccagcat ccgggcgaaa ccatggcgga
     1681 atggtttgtc gaggggctgc tcggcaggct gctcgaccct caggatgcaa ccgcgcgcac
     1741 cctgctcgat cgcgccacgt tctatatcgt ccccaacatg aaccccgacg gttcggcact
     1801 gggcaacctt cgtaccaacg ccgccggtgc gaacctcaac cgcgaatggg aaaatccgac
     1861 tgtggaaaaa agcccggaag tgttcttcgt gcgcgaaaaa atgctggaaa ccggcgtgga
     1921 tttgttcttg gacatccacg gcgacgaagg gctgcccttt atctttgttg caggaactga
     1981 aggcgtgcca aactacaatc cgcgcatttc cgcgttggaa acacagttca aaaccgccct
     2041 tttgaacgcc agccccgatt tccaagacga atacggttac gaaaaagatg cgcccggtca
     2101 ggcaaacatg accttggcaa caaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2161 gttggaaatg ccgttcaaag acaatgccaa cctgcccgac gacgatttcg gatggaacgg
     2221 tcaacggtcg ttgcgcttgg gcgaagccgc gttgtccgcc atcctcaaca tcatcggcga
     2281 tttgcgctga aacccttatt gcagaaatgc cgtctgaagc atcatgcctt tcagacggca
     2341 tttgaaaacg aaagcccgtt atgattaccc atccccaatt cgaccccgtc cttatcagta
     2401 tcggcccgct tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta
     2461 cctttctcgg cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgctcg
     2521 acgacttcct gacatggggc attttgggcg taattttggg cgggcgtttg ggttacgtcc
     2581 tgttttacaa gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag
     2641 gcggaatgtc gttccacggc ggctttttgg gtgtagttat tgccatatgg ttgttcggcc
     2701 gcaaacacgg catcggcttc ctcaaactga tggacacggt cgcaccgctt gttccgctgg
     2761 gtctcgcttc aggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg
     2821 acatcaacgc attttgggca atgggcttcc cgcaggcgcg ttacgaagat gccgaagccg
     2881 ccgcgcacaa tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc
     2941 cctcgcagct ttatcagttt gcacttgaag gcatctgcct gttcgccgtc gtttggctgt
     3001 tctctaaaaa acagcggccg accggacaag tcgcctcgct cttcctcggc ggctacggca
     3061 tattccgctt cattgccgaa ttcgcacgcc aacccgacga ctatctcggg ctgctgacct
     3121 tggggctgtc gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct
     3181 ttgtccggtt cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca
     3241 tggaaatccg gtttcagacg gtatttttac gtttggttca tggaaacaaa cgcttcaatt
     3301 cttaccgcaa cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc
     3361 ggttttttcc ccttccctgc ggacggtttg cggcttgttg atgaccgcct gccagtagcg
     3421 gtaaatgtcc gccagcgcgt aaggcaactc ggattccagc tccgccagct cgccttctgt
     3481 gaattgcagc cggatagcgc cgttttcctc ttcgtcgtaa ataccgccca atgccatgat
     3541 gggataaaac aactcttcaa attcttcatc atcggcggct tcaaaccaat cggtcggcac
     3601 aatatccaag ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc
     3661 gttttcatac agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat
     3721 cgccattgcc aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc
     3781 gtcgcccaac acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag
     3841 cgccgtcata aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc
     3901 atccaacaat tcgctcaacc gccgtttgga tgcttcggta aatttttggg aatccatcat
     3961 tttccttttc aaatgggttt tgcgccct
//
LOCUS       DAXHTY010000124         3953 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXHTY010000124 DAXHTY010000000
VERSION     DAXHTY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.124"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            308..1429
                     /gene="dnaJ"
                     /locus_tag="ACFNMK_001865"
     CDS             308..1429
                     /gene="dnaJ"
                     /locus_tag="ACFNMK_001865"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC6365061.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGSGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1624..3642
                     /locus_tag="ACFNMK_001866"
     CDS             1624..3642
                     /locus_tag="ACFNMK_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC6365062.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSELPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(3686..>3953)
                     /locus_tag="ACFNMK_001867"
     CDS             complement(3686..>3953)
                     /locus_tag="ACFNMK_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFC6365063.1"
                     /translation="GATLSAQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAE
                     QVLIWNDPTVGIGWPLQTAPLLSPKDLAGKAWAQAEKLRLTLSR"
BASE COUNT          837 a          892 c         1181 g         1043 t
ORIGIN      
        1 ttgtttggaa gggaaatggc tggaaaaaga cctgcgctat atcggggaat gaatcgacgc
       61 aaaggaaatg ccgtctgaac atgcgttcgg gcggcgtttt tataacaaaa acacttcatg
      121 gcggttggtt ttatgcctgt ctaagttttt gtgtcgtgca tacctgaaga tttcagacgg
      181 catcggttta tgctgtctga aaagtgtatt ccgtttcagt ttgtaagcta tggcagtctg
      241 tttgtcttgt gttttgcgta attgccctta ttttaagccg tgattttatt ttgaattaga
      301 tgaaaaaatg agtaatcaag atttttatgc gacgctgggt gtggcaagaa cagctaccga
      361 tgatgagatt aaaaaagcct accggaaatt ggcgatgaaa tatcatcccg accgcaatcc
      421 tgacaataaa gaggcggaag agaagtttaa agaagtacaa aaggcgtatg aaactttgtc
      481 cgacaaggaa aagcgcgcta tgtacgacca gtatggtcat gcggcgtttg aaggcagcgg
      541 acaggggggc ttcggagggt ttggcggatt tggcggtgcg cagggttttg actttgggga
      601 tattttcagc caaatgtttg gaggcggttc ggggcgcgcc cagcctgatt atcagggtga
      661 ggacgttcaa gtcggtatcg aaatcacgct tgaagaagcc gcaaaaggtg tgaagaaacg
      721 catcaatatt ccgacttatg aagcgtgtga tgtctgtaac ggcagcggcg cgaaaccggg
      781 gacatccccg gaaacctgcc cgacttgcaa aggttcgggt acggtgcaca tccagcaggc
      841 gattttccgt atgcagcaga cttgtccgac ctgccacggt gcgggcaaac acattaaaga
      901 accttgcgtc aaatgccgtg gcgcggggcg gaataaggcg gtcaagacgg tggaagtcaa
      961 tattcccgcc ggtatcgatg acgggcagcg tatccgtttg agcggcgaag gcggaccggg
     1021 tatgcacggt gcgcctgccg gcgacctgta tgtaaccgtc cgcattcggg cgcataagat
     1081 tttccaacgc gacggtctgg acttgcattg cgaactgccg atcagttttg ccacggctgc
     1141 tttgggcggg gagttggaag tgccgacctt ggacggaaag gtcaagctca ccgtccccaa
     1201 agaaacccaa accggcagga ggatgcgcgt gaagggtaag ggtgtcaaat ctttacgcag
     1261 cagcgcgacc ggcgatttgt actgccatat tgttgtcgaa acgcctgtca atttgaccga
     1321 ccgtcaaaaa gagcttttgg aagaatttga gcggatttct accggcttgg aaaaccaaac
     1381 accgcgcaag aaatcgtttt tagacaagct gcgcgatttg tttgattgat tttaagtttt
     1441 ggaaacaagc agccgtatcg gggaatctcc ctgatacggc tgttttgttt gtttaaaaat
     1501 agtttttatt ttcaatgggg tatggggcag ggtgggataa ctgtttttaa ctgttctttt
     1561 taaaacttga catcatggcg cgatgccaac aatatgtgaa cgtctgttgt caaaggaaga
     1621 ataatgaata aatctttatc cgggtcggta gaagaatacc gcgaactgac gctccgaggc
     1681 atgatactcg gtgcattgat cactgtaatt tttactgcgt ccaacgttta cctcggtttg
     1741 aaagtcgggc tgacctttgc ctcgtcgatt ccggcggcgg taatttcgat ggcggtttta
     1801 aagtttttca aaggcagcaa tattttggaa aacaacatgg tgcagaccca agcctcggct
     1861 gcgggtacgc tttcgaccat catcttcgtc ctgcccggtt tgctgatggc gggctactgg
     1921 agcggtttcc cgttctggca gacgacgctt ttatgtattg ccggcgggat tttgggggtg
     1981 atttttacca ttccgctgcg ttacgcgatg gtggtaaaaa gtgagctgcc ttacccggaa
     2041 ggtgtggcgg ctgctgaaat tttgaaagtg ggcggtcatg aagaagggga taaccgtcag
     2101 ggaggcagcg gcatcaaaga gctggcggcc ggcggtgcgt tggcgggatt gatgagcttt
     2161 tgcgccggag gtctgcgcgt gattgccgac agcgcgagtt attggtttaa aagcggtacg
     2221 gcgattttcc aactgccgat gggcttttca ctggcattgt tgggcgcggg ctatttggtc
     2281 ggactgacgg gcggtatcgc catcctgttg ggcatttcga ttgcttgggg cattgccgtg
     2341 ccgtatttct cctcacacat tccgcaacct tccgatatgg aaatggcggc gtttgcgatg
     2401 aagctgtgga aggagaaagt gcgttttatc ggtgcgggga ctattggcat tgcggcggtt
     2461 tggacgctgt tgatgctgct caagccgatg gtggaaggca tgaagatgtc gttcaagagt
     2521 tttggcggcg gtgcgcccgc tgcggaacgc gccgaacagg atttgtcgcc taaagccatg
     2581 attttttggg tgctggcgat gatgtttgtt ttaggcgtgt cgttttacca ctttatcggc
     2641 gattcgcaca ttacgggcgg catggcttgg cttttggtgg tcgtttgcac gcttttggct
     2701 tccgtcatcg gctttttggt cgccgccgcc tgcggttata tggcaggttt ggtcggctcg
     2761 tcttccagcc cgatttccgg cgtgggcatc gtgtccgtcg tcgttatttc actggttttg
     2821 ctgctggtag gcgaatccgg aggtttgttg gcggatgagg ctaaccgcaa atttttgctg
     2881 gcactgactt tgttttgcgg ctcggcagta atctgcgtgg cttcgatttc caatgacaac
     2941 ctgcaagact tgaaaaccgg ctacctgctc aaagccacgc cttggcggca gcaagtcgcc
     3001 ctgattatcg gctgtatcgt tggtgcgctg gttatttcgc ccgtgttgga actgctttac
     3061 gaagcctacg gctttactgg cgcaatgccg cgcgaaggca tggatgcggc gcaggctttg
     3121 gcagctcctc aagcgacttt gatgacgacc atcgcgtcgg gcattttcgc ccacaacctc
     3181 gaatgggtct acatctttac cggcatcgcg attggagcgg tattaatcgt cgtcgatttg
     3241 gtgttgaaaa aatcatcagg cggcaaactt gccctgcccg tccttgcggt cggtatgggt
     3301 atttatctgc cgccgtccgt caatatgccc atcgtggcag gcgcggtgtt ggcggcggtg
     3361 ttgaaacaca tcatcggtaa aaaagcggaa aaccgcgaag gccgtctgaa aaacgccgag
     3421 cgcatcggaa ccttgttctc cgccggcctg attgtcggtg aaagcctgat cggtgtgatt
     3481 atggcgttta ttattgcctt ctccgtgacc aacggcggct cggatgcgcc gctcgcgttg
     3541 aatctgcaaa actgggatgc cgccgcttct tggttgggtt tggcgttctt cgttaccggg
     3601 atgtttttct ttgcacagcg cgtactgaag gcgggtaggt agcctgtcgg aaaaaatgcc
     3661 gtctgaaacg ttcagacggc attttttatc gggaaagcgt aaggcggagc ttttcggctt
     3721 gcgcccacgc tttaccggca agatctttgg gcgacagcag cggcgcggtt tgaagcggcc
     3781 agcctatgcc gactgtcggg tcgttccata ttaaaacctg ttcggcttca ggcttgtaat
     3841 agtccgtgca tttgtacacg acttccgctg cgtcgcccaa aacgcaaaac ccgtgcgcga
     3901 agccttcggg tatccaaagc tggcattggt tttgcgcgga cagggttgcg ccc
//
LOCUS       DAXHTY010000125         3852 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXHTY010000125 DAXHTY010000000
VERSION     DAXHTY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.125"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            480..1436
                     /gene="thiL"
                     /locus_tag="ACFNMK_001868"
     CDS             480..1436
                     /gene="thiL"
                     /locus_tag="ACFNMK_001868"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC6365064.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFANVKPEDLAWKVLAVNVSDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1429..1914
                     /locus_tag="ACFNMK_001869"
     CDS             1429..1914
                     /locus_tag="ACFNMK_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC6365065.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1974..3650)
                     /gene="ettA"
                     /locus_tag="ACFNMK_001870"
     CDS             complement(1974..3650)
                     /gene="ettA"
                     /locus_tag="ACFNMK_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC6365066.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMISGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIAGQLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          793 a          936 c         1068 g         1055 t
ORIGIN      
        1 tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa ggcggtagga
       61 tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag ggataattca
      121 tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca gatgtgtgcg
      181 gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc ccgtcagttg
      241 cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt ggcgtgcagt
      301 gcggtacttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat acgggtacgg
      361 tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt tattgtttgt
      421 acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata ttttgggata
      481 tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat gatgtcgtat
      541 tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat ttgtgtttca
      601 gtgcggatat gcttttgaag gacaggcatt tttttgcaaa tgtcaaacct gaagacttgg
      661 cttggaaggt tttggccgtc aatgtttcgg atatggcggc gatgggtgcg ataccgcgtt
      721 gggtgttgct gagcgcggcg ttgcccgaat tggatgaggt atggctgaaa cggttttgcg
      781 gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc gatacgacca
      841 agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt agggcgttgc
      901 ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc ggtatggcgg
      961 cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg tttgctgaat
     1021 gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg ctgttgccgt
     1081 ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg catatcctga
     1141 ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta tccgtattga
     1201 aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc gacgattacg
     1261 agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg gcagaacggt
     1321 gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg aaggttttag
     1381 atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt ggctgatttt
     1441 aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt tggtttcggc
     1501 agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact gcctttggca
     1561 tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg tatcgtgctg
     1621 tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt cagcgaccat
     1681 ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt tgtgccgttc
     1741 aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc gctcaaaccg
     1801 tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat ggcggacgat
     1861 atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt ttaaacggtg
     1921 ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg aggttaacgc
     1981 gttaccggtt tgtatttgat gcgtttcggt ttcgtgcctt cttcgccgag tcggcgtttc
     2041 ttgtcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga gtcgccttcg
     2101 caagccaaga tatgcgtagc aatacggtcg aggaaccaac ggtcgtgcga aatcaccatc
     2161 acgctgccgg caaattccaa caatgcgtct tccaacgcgc gcagggtttc cacgtcaagg
     2221 tcgttagacg gttcatccag cagcaataca ttgccgccgc tcaacaaggt ttttgccaag
     2281 tgcagacgac cgcgttcgcc gccagacaat tgacctgcaa ttttgctttg gtcgctgcct
     2341 ttgaagttga aacgccccaa atattggcgg gcgggaattt caaactgacc aacctgcaaa
     2401 atatcgcggc cttcggcaat gttgtcgaac acggttttgt cgttttgcaa accttcgcgg
     2461 ctttggtcaa tcaggctcat tttcacggtt tgcccgattt ttacttcgcc ggaatcgggc
     2521 tgctctttgc ccgaaatcat tttaaacagc gtcgatttac ccgcgccgtt cgggccgatg
     2581 atgccgacaa tcgcgccagc aggcactttg aagctcaaat cgtcaatcag cactttatcg
     2641 ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg ctcggcaacg
     2701 ggaatgaaaa tttcctgagt ttcgttgcgt ttttggtatt cgtagttgct catttcctcg
     2761 aaacgcgcca aacgcgcttt ggacttggct tgacggcctt tggcattttg gcgcacccat
     2821 tccaattcct gcttcatcgc cttcacacgc gcggcttcgg atttcgcctc gttttccaag
     2881 cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat accgtgtccg
     2941 cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc gtgtgttacc
     3001 gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac agattccgcg
     3061 tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa caagagtttg
     3121 cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc atcccattcc
     3181 ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc gcccgtggac
     3241 gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc gtcaaaatcc
     3301 gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc ggcagccact
     3361 tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag ctcaggctct
     3421 tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc aaattcctta
     3481 tccacacccg ccataatccg caaaacggtg gacttgcccg cgccgttcaa accgagcagg
     3541 ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt tttctgcggc
     3601 ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat ggttttctcg
     3661 ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta agccagttta
     3721 tccgcaaacc ggtattgcca aaatcgggca ggattcataa aatccgctta tccctttgaa
     3781 attatataga caaaaaaaat aataatgata ggggatcgcc gccccggcaa ccatttcgga
     3841 ttttccaaag ca
//
LOCUS       DAXHTY010000126         3844 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXHTY010000126 DAXHTY010000000
VERSION     DAXHTY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.126"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            358..3556
                     /gene="nalP"
                     /locus_tag="ACFNMK_001871"
                     /pseudo
     CDS             358..3556
                     /gene="nalP"
                     /locus_tag="ACFNMK_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
BASE COUNT         1038 a         1040 c         1018 g          748 t
ORIGIN      
        1 gcagacagta cggaaccgat tcacttgtta aagaatcgtt ctctttgagc taaggcgagg
       61 caacgccgta ctggtttttg ttcatccact ataactaggg aaattcaaat taagttagaa
      121 ttatccctat aagaaaaagc cgtctaagcc ggtataaaca aaataaactc attgagctat
      181 ttgtcgaaag ttcaaatttc cattttaaaa caattagtaa aatcgagttt atcctagttg
      241 tccaagacaa cccctataat aatataattc aaaatataaa aatgggttac atctaaacat
      301 tacggaattt ttattccctc gcctgaattc tattgtcaga ttcaaggaga cctcatcatg
      361 cgaacgaccc caaccttccc tacaaaaact ttcaaaccgg ctgccatggc gttagctgtt
      421 gcaacaacac tttctgcctg cttaggcggc ggcggcactt ctgcgcccga cttcaatgca
      481 ggcggcaccg gtatcggcag caacagcagg gcaacaacag cgaaatcagc agcagtatct
      541 tacgccggta tcaagaacga aatgtgcaaa gacagaagca tgctctgtgc cggtcgggat
      601 gacgttgcgg ttacagacag ggatgccaaa atcaatgccc cccccgaatc tgcataccgg
      661 agactttaca aacccaaatg acgcatacaa gaatttgatc aacctcaaac ctgcaattga
      721 agcaggctat acaggacgcg gggtagaggt aggtatcgtc gacacaggcg aatccgtcgg
      781 cagcatatcc tttcccgaac tgtatggcag aaaagaacac ggctataacg aaaattacaa
      841 aaactatacg gcgtatatgc ggaaggaagc gcctgaagac ggagacggta aagacattga
      901 agcttctttc gacgatgagg ccgttataga gactgaagca aagccgacgg atatccgcca
      961 cgtaaaagaa atcggacaca tcgatgtggt ctcccatatt attggcgggc gttccgtgga
     1021 cggcagacct gcaggcggta ttgcgcccga tgcgacgcta cacataatga atacgcatga
     1081 tggaaccaag aacgaaataa tgtctgcagc catccgcaat gcatgggtca agctgggcga
     1141 acgtggcgtg cgcatcgtca ataacagttt tggaacaaca tcgagggcag gcactgccga
     1201 ccatttccaa atagccaatt cggaggagca gtaccgccaa gcgttgctcg cctattccgg
     1261 cggtgataaa acagacgagg gtatccgcct gatgcaacag agcgattacg gcaacttgtc
     1321 ctaccacatc cgtaataaaa acatgctttt cattttttcg gcaagcaatg acgcacaagc
     1381 tcagcccaac acactgaccc tattgccatt ttatgaaaaa gatgctcaaa aaggcattat
     1441 cacagtcgca ggcgtagacc gcagtggaga aaagttcaat ggctccaacc attgcggaat
     1501 tactgccatg tggtgcctat cggcacccta tgaagcaagc gtccgtttca cccgtacaaa
     1561 cccgattcaa attgccggaa catccttttc cgcacccatc gtaaccggca cggcggctct
     1621 gctgctgcag aaatacccgt ggatgagcaa cgacaacctg cgtaccacgc tgctgacaac
     1681 ggctcaggac atcggtgcag tcggcgtgga cagcaagttc ggctggggac tgctggatgc
     1741 gggtaaggcc atgaacggac ccgcgtcctt tccgttcggc gactttaccg ccgatacgaa
     1801 aggtacatcc gatattgcct actccttccg taacgacatt tcaggcacgg gcggcctgat
     1861 caaaaaaggc ggcagccaac tgcaactgca cggcaacaac acctatacgg gcaaaaccat
     1921 tatcgaaggc ggttcgctgg tgttgtacgg caacaacaaa tcggatatgc gcgtcgaaac
     1981 caaaggtgcg ctgatttata acggggcggc atccggcggt agcctgaaca gcgacggcat
     2041 tgtctatctg gcagataccg accgatccgg cgcaaacgaa accgtgcaca tcaaaggcga
     2101 tctgcagctg ggcggcgaag gtacgctgta cacacgtttg ggcaaactgc tgaaagtgga
     2161 cggtacggcg atgaccggcg gcaagctgta catgtcggca cgcggcaaag gggcaggcta
     2221 tctcaaccgt accggacaac gtgttccctt cctgagtgcc gccaaaatcg ggcgggatta
     2281 ttctttcttc acaaacatcg aaaccgacgg tggtctgctg gcttccctcg acagcgtcga
     2341 aaaaacagcg ggcagtgaag gcgacacgct gtcctattat gtccgtcgcg gcaatgcggc
     2401 acggactgct tcggcagcgg cacattccgc gcccgccggt ctgaaacacg ccgtagaaca
     2461 gggcggcagc aatctggaaa acctgatggt cgaactggat gcctccgaat catccgcaac
     2521 acccgagacg gttgaaactg cggccgccga ccgcacagat atgccgggca tccgccccta
     2581 cggcgcaact ttccgcgcag cggcagccgt acagcatgcg aatgccgccg acggtgtacg
     2641 catcttcaac agtctcgccg ctaccgtcta tgccgacagt accgccgccc atgccgatat
     2701 gcagggacgc cggctgaaag ccgtatcgga cgggttggac cacaacgcta cgggtctgcg
     2761 cgtcatcgcg caaacccaac aggacggtgg aacgtgggaa cagggcggtg ttgaaggcaa
     2821 aatgcgcggc agtacccaaa ccgtcggcat tgccgcgaaa accggcgaaa atacgacagc
     2881 agccgccaca ctgggcatgg gacacagcac atggagcgaa aacagtgcaa atgcaaaaac
     2941 cgacagcatt agtctgtttg caggcatacg gcacgatgcg ggcgatatcg gctatctcaa
     3001 aggcctgttc tcctacggac gctacaaaaa cagcatcagc cgcagcaccg gtgcggacga
     3061 acatgcggaa ggcagcgtca acggcacgct gatgcagctg ggcgcactgg gcggtgtcaa
     3121 cgttccgttt gccgcaacgg gagatttgac ggtcgaaggc ggtctgcgct acgacctgct
     3181 caaacaggat gcattcgccg aaaaaggcag tgctttgggc tggagcggca acagcctcac
     3241 tgaaggcaca ctggtcggac tcgcgggtct gaagctgtcg caacccttga gcgataaagc
     3301 cgtcctgttt gcaacggcgg gcgtggaacg cgacctgaac ggacgcgact acacggtaac
     3361 gggcggcttt accggcgcga ctgcagcaac cggcaagacg ggggcacgca atatgccgca
     3421 tacccgcctg gttgccggtc tgggcgcgga tgtcgaattc ggcaacggct ggaacggctt
     3481 ggcacgttac agctacgccg gttccaaaca gtacggcaac cacagcggac gagtcggcgt
     3541 aggctaccgg ttctgacgga caggaagcag acagccgcaa agatcacggt ctttgcggct
     3601 gtttcttatg aaaagaaaac cctattccaa ttgcctgctt ctattgtttc aagacttctt
     3661 ccaaagattc ggcattaatc agatgtatag cggattaaca aaaatcagga caaggcggcg
     3721 aagccgcgga cagtacaaat agtacggaac cgattcactc ggtgcttcag caccttagag
     3781 aatcgttctc tttgagttaa ggcgaggcaa cgccgtactg gtttttgtta atccgctata
     3841 ttcc
//
LOCUS       DAXHTY010000127         3831 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXHTY010000127 DAXHTY010000000
VERSION     DAXHTY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.127"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(88..906)
                     /locus_tag="ACFNMK_001872"
     CDS             complement(88..906)
                     /locus_tag="ACFNMK_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6365067.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIGNPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDVDIFRNFSL"
     gene            complement(986..2236)
                     /locus_tag="ACFNMK_001873"
     CDS             complement(986..2236)
                     /locus_tag="ACFNMK_001873"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6365068.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTTALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQSSRAQVFSLPPVSGFKNRLVIDLYPHGMDPDDPMMALLNGS
                     LNKTLRGSPEADLAQNTTPQPGRGRNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYLNTSVLKRG"
     gene            complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="ACFNMK_001874"
     CDS             complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="ACFNMK_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC6365069.1"
                     /translation="MSDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            2895..3707
                     /gene="murI"
                     /locus_tag="ACFNMK_001875"
     CDS             2895..3707
                     /gene="murI"
                     /locus_tag="ACFNMK_001875"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC6365070.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          841 a          909 c         1152 g          929 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggcgtg tccattttgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcggaaaa tatccacatc
      121 aggctgtggg ccgtacttat ttacggtatt ttcgtatgct gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggcg aatacgcaag gaaaaaaacc ggagcgcatc agcgataggg tcatgcagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaagg gatttcctat ttttggggct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttggataa aggcgtgtag tctgtcccgc ttatggtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttccg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggatcg gggctgtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactgt acacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gtccgttaaa acagataaag gaagccgatc ggacaattcg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacaa
      961 ctatattgaa acaccgcctt ttcaatcaac cccgcttcaa tacggatgta ttgaggtagc
     1021 gttggacacc cgaggcaatg gattgggcgc actgccggcg gaaggattcg ctgcccagca
     1081 gcttctcttc ggcaggattg gacaggaagg cggtttcgac caggatagac ggcatatcgg
     1141 gtgcgcgcaa aacggcgaaa ttggcttcgt ccaccctgcc tttgtgcaag tggttgagcc
     1201 tgcccaattc ttcaagcacc agtttgccga gtttgcggct gtcacgcagc gtggcggttt
     1261 gggtcatgtc gagcagggcg gtatcgacgt tgcggttgcc gctggtcggt acgccgccga
     1321 ccgcgtcggc attgttttgg gtttgttcca agaatttggc ggcggagctg gtcgcgcctt
     1381 tggtgtttaa catataaacc cccgtgccgc gcgcggaggg gctggtgaag gcatcggcgt
     1441 ggatggagac aaatacgtcc gcccgccgtg cgcgcccttt ggcgacacgc acgcccaatg
     1501 ggatgaacac gtcttcgttg cgcgtcataa agacgttgta acccaaggct tccaattgtt
     1561 ttttggtttc gcgggcgatg gagaggacga cgtgtttttc ctgcaaaccg cccgggctga
     1621 cggcgccggg gtcttcgccg ccgtgtccgg ggtcgatcat aatgacgggt ctgcgtccgt
     1681 ttctgccgcg cccgggttgg ggcgtggtgt tttgggcgag gtcggcttcg ggagagccgc
     1741 gcagggtttt attcaggctg ccgttgagca gtgccatcat cggatcgtcg ggatccattc
     1801 cgtgcggata gaggtcgata acgaggcggt tcttaaagcc gctgacaggg gggagggaaa
     1861 agacttgtgc gcgtgaggat tgtttcaggt cgatgacgag gcggacggtg gtcggcgtgt
     1921 tctgacccgc gcgtatgctg cggataaagg ggtcgtctgc catgactttc tgagacagtc
     1981 cgtgcaatac ggtattgatg tttgcgtttt gtatgtcgac gaccaacctg cccgggttgt
     2041 cgagcgtgaa gtgttggtat ttgagcgcgg ttgtgctttc cagcgtcagg cgggtgtagg
     2101 tgtgcgacgg ccatatccgt gcggcggtaa actgcggggc gcgtaccgtt ttggcaacgg
     2161 cggatgcgat ggggcttagg gcgaacagtg tgccggcggt gcggcggatg atttgtcttc
     2221 gtgtcagttt ggtcatagcg gcaggctttc acgtcctcgt tcggtatggg cggtcagcag
     2281 gcattttctg ccgccgccgt cgtgtgtcaa tgttgcggtg atgtcggcgg gcggggtaaa
     2341 ttcgcctccc tgttgcggcc attcgatcag gcagacgctg tttgcggcaa acagttcgtc
     2401 aagccccgcg tcttcccatt cttcggggaa cgagaagcgg tagaggtcga aatggtgcaa
     2461 ggtgaagcgt tccagcggat aagattcgac gatggcgtag gtcgggcttt tgactgcgcc
     2521 ctgatgaccc aatccgcgca ggatgccgcg tgtcagcgtg gttttgcccg cacccaaatc
     2581 cccttcgaga taaatgacca gcggtgcgtt taaacgggaa gaccacgccg cacccaaatc
     2641 gagtgtggca gcttcgtcgg caaggaatcg ggaaacgggg ggaaaatcag acatggaaac
     2701 ggtttgttgt aaggtttggg gtattatggg cagttttgca ggttttacaa actttgcacc
     2761 cgaggggcgg atgcttcctt tccgagcatt ataacagcca aatacgcgtt ctgctttcag
     2821 acggcatggc tgtcaagaaa aagcggcgcg tgtacaatac gcggattgta tgtttaggac
     2881 agattggaag aaggatggaa aatatcggca ggcagcgacc catcggcgtt tttgactcgg
     2941 gaatcggcgg tttgaccaat gtgcgcgcac tgatggagcg gctgccgatg gagaacatca
     3001 tttatttcgg cgatacggcg cgcgtgcctt acgggacgaa atctaaggcg actatcgaaa
     3061 atttctcgat gcagattgtc gattttttat tggaacacga tgttaaggcg atggttatcg
     3121 cgtgcaatac gattgcggcg gtggcggggc agaaaatccg tcaaaagacg ggcaatatgc
     3181 cggttttgga cgtgatttcc gccggcgcga aagccgcgct ggcaacgacg cgcaacaata
     3241 aaatcggcat catcgccacc aataccacag ttaacagcaa tgcttatgcg cgcgccatcc
     3301 acagggacaa ccccgacacg ctcgtccgca cgcaggctgc gccgctgctc gtccctttgg
     3361 tggaagaggg ctggttggaa cacgaagtta cccgcctgac cgtatgcgaa tacctcaaac
     3421 cattgcttgc agacggcatc gatacgctgg tgttgggctg cacgcacttt cccttgctca
     3481 agcccttaat cggcagggag gcgggcaatg tcgcgttggt tgattccgcg attacgactg
     3541 ccgaagaaac cgcacgcgtc cttgcgcagg aagggttgct caataccgac aacaacaatc
     3601 ccgactaccg tttttacgtc agcgatattc ctttgaaatt cagaacgata ggcgagcgtt
     3661 ttctgggtag gacgatggag cagattgaaa tggtgtcttt gggttaaaac gatgacggaa
     3721 agctgcccaa gattacagag acctaaaatt ccgtcattcc cgcgcaggcg ggaatctagg
     3781 tctgtcggtg cggaaactta tcgggaaaaa cggtttcttt agattttatg t
//
LOCUS       DAXHTY010000128         3753 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXHTY010000128 DAXHTY010000000
VERSION     DAXHTY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.128"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..221
                     /locus_tag="ACFNMK_001876"
     CDS             <1..221
                     /locus_tag="ACFNMK_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365071.1"
                     /translation="VSAVGRERNFAKVSGFNCGKIDQAEISDSEKSWLKKELGKIKDT
                     ALSTLTENAINAIPAATLITLLKKFVGL"
     gene            complement(218..724)
                     /locus_tag="ACFNMK_001877"
     CDS             complement(218..724)
                     /locus_tag="ACFNMK_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HFC6365072.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRASYTLADGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(727..924)
                     /locus_tag="ACFNMK_001878"
     CDS             complement(727..924)
                     /locus_tag="ACFNMK_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365073.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(921..1187)
                     /locus_tag="ACFNMK_001879"
     CDS             complement(921..1187)
                     /locus_tag="ACFNMK_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365074.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAQADRKNIAASLERIENKLEKVNEKLDRKADKS"
     gene            complement(1199..1537)
                     /locus_tag="ACFNMK_001880"
     CDS             complement(1199..1537)
                     /locus_tag="ACFNMK_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365075.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            complement(1534..1752)
                     /locus_tag="ACFNMK_001881"
     CDS             complement(1534..1752)
                     /locus_tag="ACFNMK_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365076.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            1765..1935
                     /locus_tag="ACFNMK_001882"
     CDS             1765..1935
                     /locus_tag="ACFNMK_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFC6365077.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(1980..2285)
                     /locus_tag="ACFNMK_001883"
     CDS             complement(1980..2285)
                     /locus_tag="ACFNMK_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365078.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTTAPSATEIKVVEKAVLPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(2182..2601)
                     /locus_tag="ACFNMK_001884"
     CDS             complement(2182..2601)
                     /locus_tag="ACFNMK_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365079.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYNRALGYGN"
     gene            complement(2573..2809)
                     /locus_tag="ACFNMK_001885"
     CDS             complement(2573..2809)
                     /locus_tag="ACFNMK_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365080.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
     gene            complement(2812..2976)
                     /locus_tag="ACFNMK_001886"
     CDS             complement(2812..2976)
                     /locus_tag="ACFNMK_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365081.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            complement(2979..3185)
                     /locus_tag="ACFNMK_001887"
     CDS             complement(2979..3185)
                     /locus_tag="ACFNMK_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365082.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            complement(3182..3727)
                     /locus_tag="ACFNMK_001888"
     CDS             complement(3182..3727)
                     /locus_tag="ACFNMK_001888"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6365083.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNNY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
BASE COUNT          850 a         1065 c          858 g          980 t
ORIGIN      
        1 gggtttcggc tgtcgggagg gaaaggaatt ttgcaaaggt ctcgggcttt aattgcggca
       61 aaatagacca agcggagatt tctgattcag aaaaaagctg gctgaaaaaa gaattaggga
      121 aaatcaagga cactgcatta agtacgctta cggaaaatgc aattaatgct atccctgccg
      181 ccaccctcat tactctttta aaaaaatttg tcggcctcta accaaacttc ttctccactt
      241 ccaccccaaa cggctccaat acctccacaa aggcaggcaa atgtttgggg tgtttttctt
      301 gcacaaacgc catcaagaac tcaatcaatt ccaaagccgt cgccagtttt gacgtttccg
      361 gcatcacacg cgcattggcc gatacggttt tcgtaaacgc atcggccagg ctggccaaca
      421 gcttggcgcg gtcggacggg ggcaaatctt cggtactcga atcctgcagc atcgtcatcg
      481 tgctgttgta ctgcaccata aaaccggcca acatcgcacg gctcaagtct tcaataccgc
      541 cgtcggccaa agtgtaggag gcgcgcatct tatcccagtc gtcgcctttt tccttatccg
      601 cacgtttcca cgcacgcgca gtggtctgcg ggatttcgca catcaaggcc gccgtttcca
      661 aagtttgctc gccgctcaca tagagccggc gtaacttttc acggatttct tgcgggtgag
      721 ccataattac agtcccattt tcgctttaag caattcccag ccgaccgtaa tcacgccgcc
      781 gcccagtgcg ccgaatgtaa tggccgtgcg tttcgtgtct tggcggattt gcgcaatttc
      841 cgcctgcatt tctttctgat ttttcagcgt ttgatcagtc ttgttttcaa tacgcgccaa
      901 ggcttctaaa atcgggtcgc tcatgatttg tctgctttcc tgtccagttt ttcgtttact
      961 ttttccaact tgttttcgat gcgctccaaa gacgctgcga tatttttgcg gtcggcttgg
     1021 gcatcctgct tggtgtggta cgagagcttg accgcatgca gctcctcttt caggtcgtcg
     1081 atacgcttgt ccgcttcttt cagacggcct gaaatgccgt tgacccaaaa ccaaaatgcc
     1141 gccgtcgcaa tcggccacag ggttctgaaa ccaaattcaa agtccattta aaaccccttt
     1201 aaaccggcac atcgccgaat acgatacgga cggcgtaacc gtcagggcga ttgctggcaa
     1261 tctccagtcc atcaccatca ttacaaacac agtaatacgc ccccaaagtt tgccaaaccg
     1321 cacgtttaaa ggtgtcatag ttcgtaattg gatattcaag gtgaaacgtc gtctgaaaat
     1381 ccttatccat gcgtaccgcg tactcaatgc ctgccttatc caacagatcg gaaacatgaa
     1441 tgacaaacgg ctcttgctcg cgtgcgcggc ttaagcccaa ttccaaatcg gcatggcgca
     1501 cagccaactg acgttcaact aattcacggt aggtcattct ttgataccca ttaaatattt
     1561 tgtccgcttg tacaatttct taacccacga aatatttaca aatgtataaa cctttgctac
     1621 aacttcgccg tcatagtgcg cattttctcg tgcagcccga aatttcgccc tggcttcttc
     1681 agggttgtcc gcccaaatgc tcaatgacca ggacttgccg ttaaagcggt aagaaaacgt
     1741 gtactcattc ataggagaaa ccttatgtat tttgaaatct ataaagacgc aaaaggcgaa
     1801 taccgttggc gtttgaaagc agccaaccat gaaatcatcg ctcagggcga aggctacacc
     1861 agcaagcaaa actgtcagca cgcagtcgat ttgctgaaaa gcactaccgc cgctacccct
     1921 gtaaaagagg tataaaatcc gctttcaccc tcagcccgcg ccctacgcgg gcttttttgt
     1981 cagtttccga ctttgcggga gtgattgccc gcccagtcgc gccaagcctg attttgattt
     2041 tccagttcgg caacatagcc gccaaactcg gcggcgtgtt ccaacagcgt ggccgtcttg
     2101 ccgtctttcg gcggattcgg gcgcaccggc gcgaccatca acgcggcagg cggtgtcggc
     2161 aagaccgcct tttcgacaac cttaatttcc gtagccgagg gcgcggttgt agagctgcag
     2221 gccgtgatgg ccaaagccgt caatacaacc gccgcctgca tttttacggt cttgagtaag
     2281 gacattttcg atttcctttt tattttccgt tttcagacgg ctgacttccg cctgtttttt
     2341 cgccaaagcc atgccgacag cgtgcgcctt gacttcatat tttttagctt ccgcacgcgc
     2401 ctgttccagc tcgcgtgcat agttttgagc cgacaacagc agggcttgcg ctttgtcttt
     2461 ttccatcttg tcaatgaccg cctgctgctt cgcaaaagcc gacttgtagc cttgatggtg
     2521 cgacaccgcc aagagcgtgc cgacaagcgc gatgatggca atcggttgcc agttattcgc
     2581 cagcagtttc acgagattca ttctcgacct cctgacgttt gacgctgacc aacgaacgcg
     2641 ccaccgcata gccgccgaca atgcccaaat acaccgccca aatctccgcc gacgggtcgg
     2701 gcagcatcac aaacttaacc gtccccgccg cgcaggcaac gtttgcccac agtttcgagt
     2761 gcgacacatt gcctgtcgct gggtttttaa aaatatccaa aatacgcatt gctattccac
     2821 acttttggtt tgcaggtgcc gtttcagcat ttcctgataa ttggccagtt cgccctccgc
     2881 aaattcaaac gccgccaagt ctgcctgttc gcttgcctca cggcttttgg ccgaccacag
     2941 cccaatcatc ttttcgtaaa acgcaacctg tcccatgatt aacgacgatt cttgcgtttg
     3001 cgcgccgcgc gtttcgcggc tgccacgcct gagtttctta aatttgggcg tggataggtg
     3061 tgtatcggac gaacatttgg catctgtata agtgcgcggt ttacctgctc attactagga
     3121 ttttctccaa tcaattttgt aagaaaatag gaagcagctt ctttcaattt accaataata
     3181 ttcatactct tgccgccccc aattccatcg caatcgcgtc cgcaatcgcg cggcagatgc
     3241 cccatttagt cgtcttaaac aaggccaaat cagtgtcgtt gctgatgaaa aaaggctcaa
     3301 acacaatgcc gccggcttgt gcataagcca ggcgcgaatg ctggcccgca ttgtcgggtt
     3361 taaagccgtc ttcgccgcgc agtttccagc cggttttctt ggcaacggct ttgctcaaca
     3421 cctgacacca gcgtttgttt ttaacggtac tcaaggcttc gatgcctgtc gccgctttgc
     3481 tgacggcagc gttggtgtga aactcaatcg ccacatccga gccgcgaatc agcttgaccg
     3541 cttcgcgcag cggcatattg cctttgcccg tgccgtcggt tttaacggtc aggccgtaat
     3601 tgttacgcag gattgaagcc acaatgttgc gcatatcctg cgccaagtcc gcctcacggt
     3661 cgctgccgtt gaccgcaccc gggtcggtgt tgctgtgtcc agcggttaag gttacggttt
     3721 tgcccattca tcatctccaa aaaatattga ttg
//
LOCUS       DAXHTY010000129         3690 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXHTY010000129 DAXHTY010000000
VERSION     DAXHTY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.129"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(153..308)
                     /gene="rpmG"
                     /locus_tag="ACFNMK_001889"
     CDS             complement(153..308)
                     /gene="rpmG"
                     /locus_tag="ACFNMK_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC6365084.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(340..573)
                     /gene="rpmB"
                     /locus_tag="ACFNMK_001890"
     CDS             complement(340..573)
                     /gene="rpmB"
                     /locus_tag="ACFNMK_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC6365085.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(823..2349)
                     /gene="farB"
                     /locus_tag="ACFNMK_001891"
     CDS             complement(823..2349)
                     /gene="farB"
                     /locus_tag="ACFNMK_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC6365086.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     ALVFFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTAGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(2373..3528)
                     /gene="farA"
                     /locus_tag="ACFNMK_001892"
                     /pseudo
     CDS             complement(2373..3528)
                     /gene="farA"
                     /locus_tag="ACFNMK_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
BASE COUNT          908 a         1034 c          918 g          830 t
ORIGIN      
        1 acttggtgct ttagcacctt agagaatcgt tctctatcct tggcagacgg tttgcagcag
       61 attcgcatac ggcagatgtt tcaagcggaa aagaaacacg ataaaaataa aaaagcctcc
      121 gaacagtcgg aggctttgct ttcaaactga aattatttca gtttggtttc tttgtacact
      181 acgtgtttgc gggcaaccgg atcgaatttt ttaatttcca gtttgccggg catagtgcgt
      241 ttgtttttgg tagtggtgta gaagtgacca gtacctgcac tggattccag tttgatttta
      301 tcgcgcattg cagtaatcct taattaaata gtgtttaaat taagcttcgc cgcgagcacg
      361 caaatcagcc aatacgacat caatgcctac tttgtcgatg gtacgcagtg cagcgttgga
      421 aacgcgcagg cgaacccagc ggttttcact ttctacccaa aaacgacgtg attgcaagtt
      481 gggcaaaaaa cggcgtttgg ttttgttgtt ggcgtgcgat acgttgttgc cggacatcgg
      541 gcgtttgccg gtcactttgc aaactcgtgc cataatcagt ctccaaactt ctaaaatagt
      601 aaaacgcgat ttataccacg aaaaacagtg ttagttcaag tgttttaaag aaaaacggct
      661 gttttgcgcg gataatcctt ccgattgtcg catctgcgga acgtttgcag gtgcgaaaca
      721 gatggggcgc gattatagcg gatttcggga actgctgcat tcaaaatgcc gtccgaacat
      781 atttccagta ttttcagacg gcatttcaag ttttcaaatc cctcaatgtc caccgccgcc
      841 gccgttgtgg aacggcggtt ttgccagcca tatgacagga atcatgatga tgaacagcag
      901 gctgcccgcc ataaagattt cgttcgagcc gataataaaa ccctgctggg taatggtatt
      961 gttgataata cctgcggttt gaatgtcgga aacgccgtgc tgggacaaat gcgccgccgt
     1021 ttcgtgcaat gtcgcggaat atggcgtgat gtgttcggca aagcgtgtat ggtgcaacgc
     1081 ttcgcgccgt tcccacaggg tgctgacgac ggatacgccg acaccgccca tcagcacgcg
     1141 caagaaattc gacaggctgc ctgcggcggc aatctgtccg cccttcatgt gcgacagtgt
     1201 gatggtggtc agcggcagga agaacatggc aacgcctgcc ccctgccaaa actgcggcca
     1261 gatgacgttg ccgatatcca tgtcggcata aaaatcggtg cgccaataga aagtaaaggc
     1321 aaaggtcagg aagctggcgg ttacgagcag gcgcatatcg attttattgc cgaacctgcc
     1381 gattaacgga gacaggaaga cgggcaggat gccgacgggt gcggcggcaa gccccgccca
     1441 cgtggaggta tagcccaggt tggtctgcaa cactaacggc agcaaggtca gcgtccccat
     1501 atacaccata aaacccaatg atgtggcaat tacgccgacg gtaaaattcc gatctttaaa
     1561 cagcgataaa tcgacaatcg gatatttttc tcccaattcc caaacaataa aatacgacaa
     1621 acacaccagt gcgactacgc ccaaggtaat gatttctcca gaggcgaacc agtcgagttc
     1681 cttacccctg tccagcatca tctgtaacgc gccgataccg actaccatca atgtaagccc
     1741 gacatagtcg gtcggcattt taacggtttc cgtttcccga tatttcaaat gtttccatgt
     1801 aatccatgcc gatatgatac cgatagggat attaatgaag aaaatccaac cccaatgcca
     1861 gtttccggaa atccagccgc cgagtatcgg cccgagaaca ggggcgacaa cgacggtcat
     1921 tgcccacaat gccagtgcca gcgtccgttt tgcgggcgga taggatgcca ttaacaggct
     1981 ttgcgacagg ggaatcagcg gcccggcgat aaaaccctgc aagatgcgga aaaacaccaa
     2041 agcctgaagg ttgggcgcga tgccgcacag ccatgatgtg atgacgaaac cgacagcggc
     2101 ggcggtaaac aatttgacct cgccgatacg ttttgccaaa aagcccgtta acggcacgga
     2161 aacggcgttt gccacagcaa aagaagtaat gacccacgtc ccctgcgtgg ttgccgcacc
     2221 gaggttgccg gcgatgacgg gaacggcgac attggcgata gtcgtatcta aaacttccat
     2281 aaatacggca agccccaaag acagcgtaac ccacgccaat gccgcaccct taagcggtgg
     2341 ataatccatt gtgtcctccg tttcagacgg catcagggcg cggattgccc gaggatttca
     2401 tcgaccgtcc gatcgacttc cgaccagtcg gtactttcca tttccggcaa tgccgcaccc
     2461 ggcgtttttg aaacaggcgc gcctgcggcg gaagtatcca ctttaaccgt catcgacaaa
     2521 ccgatacgca acggatgcct gtccacatct tcgcggttca gcacgatacg gacggggacg
     2581 cgctgcacca ctttaatcca gttgcccgtt gcgttttgcg ccggaatcag cgaaaacgcg
     2641 ctgcccgtac ctgccgaaaa ccctgccacc ctgccgcgat aaacaatttg tttgccgtac
     2701 aaatcggaca ccagttcggc aggctgtccg attttcatat gccgcaactg cgtctcttta
     2761 aaattggcat ccacccacac atccgacagc ggcaccaccg ccatcagcgg cgcgcctgcc
     2821 gccacctgct gcccgacctg caccgaacgc ttcgccacct gaccgtccgc cggcgcgcgg
     2881 atttgcgtcc gctgaaggtt caaccacgca tctttcaacc tgccgattgc cgtctgaacc
     2941 gccggctgtt cgcgcaaaga aacatctccg cccaaagccg cgcgtgccga agactcttcc
     3001 gccaaagccg ctttgaccgc cgcctgcgcc tgagacactg ccgcacgggc gtgtgccagc
     3061 tcttcggcgg acaccgcgcc cgattccgcc aaagcagacc ggcggcgcaa atcatcctgt
     3121 gcgcgtgcca aatccgcccg gcgcaaggca acctgcgccc ccgcctgcga agtggcggca
     3181 ttttgccggc ggttttgccg caccgcctga accagctcgt tttttgcccg ctcgtaagcc
     3241 agcacatcat tatcgtcgtc caataccgcc agcacgtcgc cttttttcac ggcatccgta
     3301 tcgtcgtgca aaaccttccg caccgtaccg cccttttgcg gcgtaacctg aaccacgcgt
     3361 ccggcaacat aagcgtcttc cgtttcctct tcgtgctgcc accataaaaa aaaacgccga
     3421 cccggcggct acggcggcaa gcgcgaacag cagcgtcaat gccgtcaggc ggcgtttgcg
     3481 tttggcttgc gtgttttgaa gttttgtttc gtccgtgtgc gtatccataa gattgggttc
     3541 cgaatttcct gatttcatat aaacaatgtt ctttcaatag gaagcagatt ctatatccgc
     3601 gcaataacgc aatcaagtca gtattttaga tgcaatattc aaaaaacaac gcttcaggcg
     3661 gcaaaactga aaatatattt cgtttatata
//
LOCUS       DAXHTY010000130         3601 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXHTY010000130 DAXHTY010000000
VERSION     DAXHTY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.130"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..305
                     /locus_tag="ACFNMK_001893"
     CDS             <1..305
                     /locus_tag="ACFNMK_001893"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365087.1"
                     /translation="YATAYWPFSVRESCTSNANPPYLEIDPKTISESNVANYVLFPNP
                     EQITKEFCSSKIDDDLMLDEEFIPIEIIGENELIPCGYLDFDIEYWSNYLFSEKNQ"
     gene            398..643
                     /locus_tag="ACFNMK_001894"
     CDS             398..643
                     /locus_tag="ACFNMK_001894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365088.1"
                     /translation="MHIYAGKCKKDDIGFFVEKEDKNVSFDCNGVSGGIVMRMIDGHS
                     EWVGIAVRANNDIIRFIPYYLVAKSIIQNILNRNQQA"
     gene            762..833
                     /locus_tag="ACFNMK_001895"
                     /pseudo
     CDS             762..833
                     /locus_tag="ACFNMK_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1308..2024)
                     /gene="trmB"
                     /locus_tag="ACFNMK_001896"
     CDS             complement(1308..2024)
                     /gene="trmB"
                     /locus_tag="ACFNMK_001896"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC6365089.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(2146..3549)
                     /locus_tag="ACFNMK_001897"
     CDS             complement(2146..3549)
                     /locus_tag="ACFNMK_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6365090.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGARPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTTAKNNNPHGQYLLAQYCQYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVYQFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLTAYQKLISDSALNHPEQYGGIK
                     DSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAQKDSMALYNLACIHYNGHGVEPDKEKACRYLQEAINNGY
                     GQKSFLQELLQQWQNAV"
BASE COUNT          781 a          769 c          995 g         1056 t
ORIGIN      
        1 cgtatgctac cgcatactgg cctttttctg ttagggaaag ttgcacttca aatgcgaatc
       61 cgccatatct tgaaatagat cccaaaacta tatctgaatc taatgtagct aattatgtac
      121 tttttcctaa tcctgagcaa attacaaaag agttttgtag ttcaaaaatt gatgatgatt
      181 taatgcttga tgaggaattt atccctattg agattattgg agaaaatgaa cttataccat
      241 gtgggtattt ggattttgat atcgaatatt ggagtaatta tttgttttca gaaaaaaacc
      301 aatagaaaat gaaattcttt tagtatgcgg gttccctttt tcagataatg aaatagatta
      361 tactgatgat ggtgagatta aaaaagtatc gcatcaagtg catatatatg ctggtaaatg
      421 taaaaaagac gatataggct tttttgttga aaaagaagat aaaaatgttt catttgattg
      481 taatggtgtt agtggtggaa ttgtgatgag aatgatagat ggacacagtg agtgggttgg
      541 aattgctgtt agggcaaata atgacatcat tcgttttatt ccttattatc tcgtagcgaa
      601 atcaataatc cagaatatct taaatagaaa tcaacaagcg taggtcggat acttgtatcc
      661 gacaaaacct tcaacattcc cgtcgttgca atccattgca gctatgtccg aaatgtcgga
      721 ttcgaaaatc cgacctacaa aatcattccg agcataacac tatgaaataa cgtcgttttt
      781 accgcaatga cggcacttac ttttttacgg ttgcaaccaa taaacggcag caagcgtagg
      841 gtgggcactt gttgcccacg cgtttatctc cgaaaggcag caactgtagg tcggatactt
      901 gtatccaaca aacctttaac attctcgttg ttgcaatcca ttgcagcaat gcccaaaatg
      961 tcggattcga gaatccgacc tacaaaatca ttccgaacat aatactatga aataccgtcg
     1021 ttttaccgca atggcggcac ttactttttt acggttgtaa ccagcaagcc gtagcctaca
     1081 tcacaccaac cgcgtgcgta gctatcacat actacctgcg ggcggcacaa actttaaaac
     1141 cgatgtgcag gttaaaacct acaaggatgc gtgcggtaca cacgcgtttt gctgttcgca
     1201 ccattgcttt gaactttata tcgaaacatc actcgctgct ttatcttgaa aaaatgccgt
     1261 ctgtaagcaa acgtgcttca gacggcattt ttttatccgg cggtctgtta tccgatccgt
     1321 ttgaatacca agtcccaaac gccgtgcccg aggcgtttgc cgcgcgcttc gaatttggtt
     1381 tcggggcggt agtccggggt gggggcgtag tctgccgccg tattttgcag gctgtcgaag
     1441 ctactgagga cttcaagcat ctgctgtgca tattcttccc agtctgtcgc caggtggata
     1501 tagccgccgg ttttgagttt gggcagcagt ttggcgatga agggggcttg tatcagacgg
     1561 cgtttgtggt ggcgtttttt gtgccacggg tcggggaaga atatgtggat gccgtcgagc
     1621 gagccgtctt gcagcatatt ttcgacgact tctacggcat cgtgccgcat cacgcggatg
     1681 ttttccaaat ggttttcgtt gatgagtttg agcaggttgc ctacgccggg accgtgtacg
     1741 tcgatggcga ggaagtcggt ttcgggcagg cggcgggcga tttctgcggt tgccgtcccc
     1801 ataccgaagc ctatttcgag aattttgggg cggctgcttc cgaaacgggc atcgagatcg
     1861 gccggtgttg cttggtagtc gatgccgaag ctgtcccata aggtatcgat ggcgcgttgc
     1921 tgcgcggcgg tcatatggct ttggcggagg acgaagctgc ggatgctgcg gggatgttcg
     1981 gcttgccagt tgttttgtgt ttggttttcg gcggtgtctg tcataatgct ttcgtgcgct
     2041 tgtgcaatca ggatgccgtc tgaatgtgaa atgcctgttc ggacggcggg aagggaaagg
     2101 ggcgattata cctgtttcgt ttttgcgggg taaatgtacc agtgttcaga cggcattttg
     2161 ccactgttgc aacagttctt gcagaaagct tttttgcccg tatccgttgt ttatggcttc
     2221 ttgcaggtag cggcaggctt tttctttgtc cggctcgacg ccgtgtccgt tgtaatggat
     2281 gcaggcgagg ttataaagtg ccatgctgtc tttttgcgcg gcggcttttt caaaccattt
     2341 gcgtgcttca ttgtagtcgg cggtcatacc ctgtccgaag taatacatgc tgcccagatt
     2401 ggtttgggct ttgctgtgtc cgagttctgc ggcttctgtg tagagtttga gcgccgccgc
     2461 gtattcgggc gcgcattgta atccgtaatg cagggcttgg gctttttgat aaagctgttc
     2521 tgccctttgc cgcctaatgg cggaatcttt gatgccacca tattgctcgg gatggtttaa
     2581 cgcactgtcg gatatgagtt tctgataggc ggtaaggctg ccggcttcgg ctgcctgccg
     2641 gtaataatgc cgggcttttt ccgtatcggg ggctatgccc aaaccgtagc ggcagatgtc
     2701 gcccagtatc cgcaaacctt cgggatggcg ttcggcggcg gctgcttcgg cgtgatgcag
     2761 ggcaagtttg tagtttcttt ccggatgctt gccttgcagg tagatgtcgg caagtgcggc
     2821 atgggcatcg gggtcatttt cctgtgccgc ctgttgaaac tggtaaacgg cttcatcggg
     2881 agccgtaggt aggatgagct cggcaagcgg ggtgtaggcg ggaatgtatc cttgttgtgc
     2941 ggcggcgcgc aaatgattga tagcttgggt cgtgtcggtt ggtgtacctt gtccgaaacg
     3001 gtattgaagg ccgagctgcc attgtgccgc cgccaagcct tgtgctgcag cctcgcggta
     3061 gagaaggtgt gccgtttcaa aatccggtgg cgtaccgtac tggcagtatt gggcaagcag
     3121 atattgtccg tgggggttgt tgttttttgc ggtcgttttt gcccatttca gggcttcttc
     3181 aaaatgtcgg gacgcataca gaaggtacat caggcggact tgtgcttcgg gcagtccttc
     3241 ctctgccaac ggtctgtaaa gcggtatcag ggtttccggg cgcgcgcctt ctaattcttg
     3301 gatatatgcc agattgtaac gcgcgtaggg atgtccctgt tcggcggcga tgcggagcat
     3361 ttccatacct tgttcgcggt cttggtaggg gctgtccgcg tccatcaggt gttttgccaa
     3421 ctcgaatgcg gcggcgggat gacctgccga ggccgcttct gtcagcagtc ggcggacttc
     3481 ggtaaagtta tattgttttt gagacaggcg ttctaaggcc tgccgatagg gtaagtcggg
     3541 attgttcata tcagatcgtt ttccgttttg tcgaatcatc gttattgtgt tatgaagtta
     3601 g
//
LOCUS       DAXHTY010000131         3558 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXHTY010000131 DAXHTY010000000
VERSION     DAXHTY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.131"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(268..906)
                     /locus_tag="ACFNMK_001898"
     CDS             complement(268..906)
                     /locus_tag="ACFNMK_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC6365091.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            1161..2003
                     /locus_tag="ACFNMK_001899"
     CDS             1161..2003
                     /locus_tag="ACFNMK_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC6365092.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2069..2731
                     /gene="exbB"
                     /locus_tag="ACFNMK_001900"
     CDS             2069..2731
                     /gene="exbB"
                     /locus_tag="ACFNMK_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC6365093.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            2734..3168
                     /locus_tag="ACFNMK_001901"
     CDS             2734..3168
                     /locus_tag="ACFNMK_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC6365094.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
BASE COUNT          936 a          826 c          999 g          797 t
ORIGIN      
        1 gggtttcggc tgtcgggagg gaaaggaatt ttgcaaaggt ctcagacggc aataaatcca
       61 gcatttgatt ggcaagtttt ggcgatgtga aaacctcatc attagacaga ctggagtttc
      121 ttaatcagtg tttcagttaa tgaaaggtat tatcatgcaa cttggttcta ataatgatga
      181 aaaaaataag attacactac aggaaaacgc ggcagggcaa tatgtcctgc cgcgttttct
      241 ggagcgttta tgccgtctga aagccagtca gacgaacatc tgcacaaacg caatcaccag
      301 ccgcacaatc ggtgcaataa acgcacccaa aaccccggtc agcatcagca gtaggataat
      361 ccacgtccca taaggttcga ttttgcggaa cgcttgcgaa tatttcgccg acaggaaggt
      421 gtcgatgaaa atgccgccgt cccaaggcag gatggggatg atgttgagcg cgaacagaat
      481 cgcattgatc agaataccgt agtttgccat ttgagccaac ggcatctgat acgccccgcc
      541 gacatacgga gtcagcacca aaaccacgcc ccacagaaca gccatcgcca gattcgacag
      601 cgggccggac gcggcaacgc aacgccaggc aaggcgcggg ttgcggaagt tgcgcgaatc
      661 gataggaatc ggacgcgccc agccgaacag gaagggcgtg aacatcaaag taagcagcgg
      721 tacgatgatt gtgccgacca aatcgatatg gggcaggggg ttcagtgtca gcctgccgta
      781 ttgttcggca gtgttgtctc cccagtagcg cgccgtatag ccgcgcgcca cctccctgac
      841 ggtaatggcg agcagcacgg gcaggacggc aagcagaaac acgcccaaat caaaattttg
      901 aaacatggtt ttcctttgaa cagatgtggt gtgcagcccg aaacggctga cagacagaag
      961 cggcaatata tcatctaacc tgattttatt ggaataagaa atcagacgga atcatttttt
     1021 ctgccggccc gttatctgat gatttgctgt aatacagcgt ttaaacacgg tttgcctgtg
     1081 aacggaacag acattcatat gaccaaaaat tgtaaaaata ggttgcaaat aggaattgtt
     1141 gctattatta tgcccgaatt atggataaag aacgaatttt aacccccgca gtcgtgtttt
     1201 ccgtcgcact gctgcatttg gcaatggtgg cattgctctg gcaggcgcac aagctgcccg
     1261 tgatagagtc aggaaatgtt atcgaatttg tcgatttggg tgattttggc ggaggggacg
     1321 gtgcacccga aggcgcaggt gcgcctgctg cgcctgagcc gcaacctgag cctgagccgc
     1381 ccaaacctgt cgagccgccc aagccggttt tgaagccggt ggttacgaaa aaggcggatg
     1441 cggatattca gcagcctaag gaagagccga aacctgaaga aaagccgaaa cccgaagaaa
     1501 aaccgaaacc agagcctaag ccggaagcga agcctgtccc gaaaccggcg gaaaaaccgg
     1561 tcgagaagcc gtctgaaaaa cctgccgaac atcccagcaa tgcttctgcc aaagcagaca
     1621 gcgagcaggg taatggggaa gataagggaa ccggtaccaa aggagacgga acggggcgcg
     1681 gagaaggcag cggtaaaggt agcggcggtg tcaaaggcga acacggggaa ggagccggca
     1741 gcagcaaagg caatccttta cgcgccaacg gcagcattcc gcgcccggct tatcccacgc
     1801 tttctatgga gaatgacgag cagggtacgg ttgttttgag cgttttggtt tctccgggcg
     1861 gtcatgtcga gtccgttaaa atcgtgaaaa gcagtggttt ttcccggttg gacaatgcgg
     1921 cacgcaaggc ggcgcaaaac gggcattttc aagccaatgc ctggacggag tttaaagtcc
     1981 ccgtcaagtt tgaattgaat taggcggttg ccgcagcggt ttgcggcagc ttggatgttt
     2041 gattggtttt ttcagtgttg gaatcattat gaatttgaaa ttagtatttg aatcgggcga
     2101 tcccgtcctg atcggtgtgt ttgtgttgat gctgttgatg agtatcgtaa cgtggtgttt
     2161 ggttgtcttg cgctgcatca agctgtatcg ggcgcgcaaa gggaatgccg ccgtcaaacg
     2221 gcatatgcgc gatactttgt cgctgaacga cgcggtcgaa aaagtgcgcg ccgtcgatgc
     2281 gcctttgtcc aaactggcgc aagaggcatt gcagtcttac cgcaactacc gccgaaacga
     2341 agcgtccgaa ctggcgcagg ctttgccgtt gaacgagtat ttggtcattc aaatccgcaa
     2401 cagtatggcg cagattatgc gccggtttga ttacgggatg accgcgcttg cctccatcgg
     2461 cgcgaccgcg ccgtttatcg ggctgttcgg cacggtttgg gggatttacc acgccctgat
     2521 caatatcggg caaagcgggc agatgagtat tgcggcggtt gccggcccga ttggcgaggc
     2581 actggtggcg acggcggcgg gtttgttcgt ggcgattccg gcggtgttgg catacaactt
     2641 cctcaatcgc ggcacaaaaa tactgaccca ggatttggat gcgatggcgc acgatttgca
     2701 cgtccgcctg cttaatcaaa aggatagctg attatggcat ttggttcgat gaattccggc
     2761 gacgattctc cgatgtccga catcaacgtt acgccgttgg tggacgtgat gctggtgttg
     2821 ctgattgtgt ttatgattac tatgccggtg ctgacgcatt ccatcccttt ggaactgccg
     2881 accgcgtccg agcagacaaa caagcaggac aaacagccta aagaccccct gcgcctgacg
     2941 attgatgcga acggcggcta ttatgtcggc ggggattctg caagcaaagt ggaaatcggg
     3001 gaagtggaaa gccgtctgaa agccgccaag gagcagaatg aaaacgtgat tgtggcgatt
     3061 gcggcagaca aggcggtgga atacgattat gtaaacaaag ctttagaagc cgcccgtcag
     3121 gcaggaatca ccaaaatcgg ttttgtaacc gaaaccaagg cgcaataaag ttcgacgctt
     3181 gaacgaatgc cgtctgaaag tctttcagac ggcatttctt atgtttaatc agctatatat
     3241 agttgattaa aacaaaaatg agacgataga gcgccgtctc catttctgcg caggcgggaa
     3301 tacaaacatc tgatttttct tttattttca aacattactg aaatgggtaa gtctggagtc
     3361 ctgcctgcgc gagaataatg aaaatgtgca tttctaattt ttatagtgga ttaacaaaaa
     3421 tcaggacaag gcaacgaagc cgtagacagt acaaatagta cggaaccgat tcacttggtg
     3481 cttcagcacc ttagagaatc gttctctttg agctaaggcg aggcaacgcc gtactggttt
     3541 ttgttaatcc gctatacc
//
LOCUS       DAXHTY010000132         3462 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXHTY010000132 DAXHTY010000000
VERSION     DAXHTY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.132"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            112..1341
                     /gene="sstT"
                     /locus_tag="ACFNMK_001902"
     CDS             112..1341
                     /gene="sstT"
                     /locus_tag="ACFNMK_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC6365095.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADFGRQRNRAE"
     gene            1607..3091
                     /locus_tag="ACFNMK_001903"
     CDS             1607..3091
                     /locus_tag="ACFNMK_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6365096.1"
                     /translation="MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVAE
                     DAGEEVLPKEKKDEEAVGGAPQADTQDATAGENSQDMAAVSAENTGNGGSATTDKPKN
                     EDEGPQNDMPQNAADTDSLTPNHTPAPNMPTGDMGNQAPDSGESAQPANQPDMANAAD
                     GIQGDDPSVGENAGNTAAQGTNQAENNQAAGSSDSAPASNPAPANGGSNFGRVDLANG
                     IKLDGGSENVTLTHCKDKVCGSDFLDEEAPSKSEFESLDDSGRINKYKKDGQDKFTNL
                     VATEVKANGTNKYVIIYKDKSTSSVRVRRSARSRRSLPAEMPLIPVNQADTLIVDGEA
                     VSLTGHSGNIFAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGEVLH
                     FHTENGRPSPFRGRFAAKVDFGSKSVDGIIDSGDDLHMGKQKFKAAIDGNGFKGNWTE
                     NGGGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(3166..3381)
                     /locus_tag="ACFNMK_001904"
     CDS             complement(3166..3381)
                     /locus_tag="ACFNMK_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365097.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(3374..>3462)
                     /locus_tag="ACFNMK_001905"
     CDS             complement(3374..>3462)
                     /locus_tag="ACFNMK_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6365098.1"
                     /translation="VTMEKDLRRMLLFFKREAYVVILERDRV"
BASE COUNT          843 a          844 c         1001 g          774 t
ORIGIN      
        1 tgatttttgt taatccacta taaaacacag ccgaaaaata aaaaaacaca gagaatgagt
       61 tttcttttaa aatacaacat tttttaacat atcacaacat aaggaagaat tatggcgttc
      121 ggcaaatcct tatttcatgc gatcggccgc gtcagcctgg tcaggcagat tgccgccggt
      181 ttggcgttgg gcatcgtaat cggttcggtt tccccgcaac tgggcttggc ggcaggcttg
      241 ttcggcagcc tgtttgtcgg cgcgctcaaa gcggtcgcgc cggttttggt atttattttg
      301 gtggcggcca caatcgcgca gcaccaaaaa ggcaacaagg cgcatatcag gccgattatc
      361 gtcctttacc tcatcggtac gttttccgca gccctgaccg ccgtcatcgc cggtatggtt
      421 ttcccgacgc acattgtttt ggcgggcgcg ggcgatgtgt ccgccgcgcc gccttccggc
      481 attgtggaag tattgaaatc gctgctgatg aacctggtcg ccaacccgat taacgcgatt
      541 gccaatgcca actatatcgg cattttggct tgggctttgg ttttgggcgc ggcgttgcgg
      601 aatcacggtt cggacgttac gcggcaggtc gttgccgatt tggcggaagc ggtttccacc
      661 gtcgtgaaat ggattatccg ttttgcccct ttgggcattt tcgggctggt gtcgtccaca
      721 atcgcggaga cgggtttcgg cgcgctggcg ggttatgcga agctgcttgc ggtgctgttg
      781 ggctgtatgg cgtttatcgc gctagtggtc aatcccgcca tcgtgtggtg gaaaatccgc
      841 cgcaatcctt atccgctggt gtttacctgc ctgcgcgaaa gcggcgtgta tgccttcttt
      901 acccgttctt ccgccgccaa tatccccgtg aatatggctt tggcgaaaaa actggggctg
      961 cacgaagaca cttattcgat ttccatcccg ttgggggcga ccgtcaatat gggcggcgcg
     1021 gcgattacga ttaccgtttt ggctatggcg gcggcgcaca ctcaaggcat acaggttgat
     1081 tttgccaccg cgctgctgtt gagcctggtg gcgacggtca gcgcgtgcgg cgcgtccggt
     1141 gtggcaggcg gttcgctgct gctgattccg ctggcgtgca gcctgttcgg catcagcaac
     1201 gatgtcgcta tgcaggtggt cgccgtcggc tttatcatcg gcgtgattca ggattcggcg
     1261 gaaactgcgc tgaactcttc gaccgacgtt ttgtttaccg ctgccgccga ttttggccga
     1321 cagaggaacc gggcggaata aaccaagcta tgccgtctga agcccgtttg gcgttcagac
     1381 ggcatatttt tacaatttta taggcatacg gcggaaacca taccggcttt gcttgattga
     1441 atatgtttca acacacaggg cggcacataa agcatcgccc tatgtgttgc cctgatttgg
     1501 aagggttgcg cccttcccaa ataaagtctg attccaccgc cccgaaggac ggatgtccaa
     1561 gtggcggggt ttaaatcgaa aaggaaacac gatgaaggag atgatgatgt ttaaacgcag
     1621 cgtgattgca atggcttgta tttttgccct ttcagcctgc gggggaggcg gcggaggatc
     1681 gcctgatgtc aagtcggcgg acacgctgtc aaaacctgcc gctcctgttg ttgctgaaga
     1741 tgccggggaa gaggtgctgc cgaaagaaaa gaaagatgag gaggcagtgg gcggtgcgcc
     1801 gcaagccgat acgcaggacg caaccgccgg agaaaacagc caagatatgg cggcagtttc
     1861 ggcagaaaat acaggcaatg gcggttcggc aacaacggac aaacccaaaa atgaagacga
     1921 gggaccgcaa aatgatatgc cgcaaaatgc cgccgataca gatagtttga caccgaatca
     1981 caccccggca ccgaatatgc caaccggaga tatgggaaac caagcaccgg attccgggga
     2041 atcggcacaa ccggcaaacc aaccggatat ggcaaatgcg gcggacggaa tacaggggga
     2101 cgatccgtcg gtaggggaaa atgccggcaa tacggctgcc caaggtacaa atcaagccga
     2161 aaacaatcaa gccgccggtt cttcagattc cgcccccgcg tcaaaccctg cacctgcgaa
     2221 tggcggtagc aattttggaa gggttgattt ggctaacggc atcaagcttg acggcggttc
     2281 ggaaaatgta acgctaaccc attgtaaaga caaagtatgc ggtagcgatt tcttagatga
     2341 agaagcaccg tcaaaatcag aatttgaatc gttagatgat tctgggcgaa ttaataaata
     2401 taaaaaagat ggacaggata aatttactaa tttggttgcg acagaagtta aagctaatgg
     2461 aactaacaaa tatgtcatca tttataaaga caagtccact tcatctgtgc gagttaggcg
     2521 ttctgcacgg tctaggaggt cgcttccggc tgagatgccg ctgatccccg tcaatcaggc
     2581 ggatacgctg attgtcgatg gggaagcggt cagcctgacg gggcattccg gcaatatctt
     2641 cgcgcccgaa gggaattacc ggtatctgac ttacggggca gaaaaattgt ccggcggatc
     2701 gtatgccctc agtgtgcaag gcgaaccggc aaaaggcgaa atgcttgctg gcacggccgt
     2761 gtacaacggc gaagtgctgc atttccatac ggaaaacggc cgtccgtccc cgttcagagg
     2821 caggtttgcc gcaaaagtcg atttcggcag caaatctgtg gacggcatta tcgacagcgg
     2881 cgatgatttg catatgggta agcaaaaatt caaagccgcc atcgatggaa acggctttaa
     2941 ggggaattgg acggaaaatg gcggcgggga tgtttccgga aggttttacg gcccggccgg
     3001 cgaggaagtg gcgggaaaat acagctatcg cccgacagat gcggaaaagg gcggattcgg
     3061 cgtgtttgcc ggcaaaaaag agcaggattg atgctgatgc cgcctgaagc gggggcgttc
     3121 agacggcatt gtgatgtttg gggtggggtc cggttcaagt ccgcatcagt tgataaaccc
     3181 gtcgtctttc attttctttt cggaaaaata ctggccgaat accagcaggg cagcgaggat
     3241 gatgacaaac agtcccgcca tagtcagagt gattttgaaa aacaattccg cttcgagaaa
     3301 agaaaaccag agttgcacaa tcagcagcag cacccagccg gcaaacagtc cgagacaggc
     3361 ataaacgccg agcttaaaca cggtcccgct ccaaaatgac gacgtaggcc tcgcgtttga
     3421 aaaacagcag catacggcgc aaatcttttt ccatcgttac ga
//
LOCUS       DAXHTY010000133         3382 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXHTY010000133 DAXHTY010000000
VERSION     DAXHTY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.133"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            36..704
                     /locus_tag="ACFNMK_001906"
     CDS             36..704
                     /locus_tag="ACFNMK_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC6365099.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPAQVKKDGTVNNKCDSGKKGQGMLAFGDKNGNK
                     TYDGDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYTK
                     DQHLTKQSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCPRNDRRTV
                     CQYK"
     gene            734..1348
                     /gene="pilV"
                     /locus_tag="ACFNMK_001907"
     CDS             734..1348
                     /gene="pilV"
                     /locus_tag="ACFNMK_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC6365100.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHHTLSAVDSDFAVDA
                     VKSKKDLAKAQLDRFGYELKNALPDAAAIYYAICKDSLGVAPTLSAGSTFSSNCDASA
                     NGDTLIKVLWVNDSAGDSDIAHTNLETNGNNIVYTYQARVGGRE"
     gene            1345..2283
                     /locus_tag="ACFNMK_001908"
     CDS             1345..2283
                     /locus_tag="ACFNMK_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC6365101.1"
                     /translation="MKRKMLNVPKGNYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLSAQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVIFDTTQQNSP
                     FSLKRNSTNKLIPITESSNINYQNFFQVGSALIFQYGIDDLDASAETVVVSSCSKIAK
                     LGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGRIAGEEGLFRFQLDDKGK
                     WGNPQLLAKKIRHMKVQYIYVSDCPEDDDAGKEEKFKYTGTFDSSTNAVTPAGVEVLL
                     SSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2262..2855
                     /locus_tag="ACFNMK_001909"
     CDS             2262..2855
                     /locus_tag="ACFNMK_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC6365102.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCTAKSTGLCIDNKGMEYKKGKGSVSKPPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTLVVLQSYVSNNDE"
     gene            2860..3333
                     /locus_tag="ACFNMK_001910"
     CDS             2860..3333
                     /locus_tag="ACFNMK_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC6365103.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNTVKQFILKNPLDDNQTIKRKLEIFVSGYKMNPKIAKKYSVSVRFVDKEKSRA
                     YSLVGVPKAGTGYTLSVWMNSVGDGYKCRDKASAEAYSETLSADAGCEAFSNRKK"
BASE COUNT          971 a          667 c          851 g          893 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gtcctatgtg tacacgaaaa caacaaggtt
       61 tcacgctaac agagctgctc atcgtgatgg tcattgcagc cattatggcg atgatagccc
      121 tccccaatat gagccaatgg attgcatccc gccgcattgc cagtcatgcg gagcggattg
      181 ccaacctttt gcgtttctcc aggggcgaag ccgtccggct caatctccct gtctatatct
      241 gtcctgctca agttaaaaaa gacggtacgg tcaacaataa atgtgactcc ggcaagaagg
      301 ggcagggaat gttggctttt ggcgataaga atggcaataa gacatatgac ggcgatacgg
      361 cggatgttct tctccgcagt gtggtattga atgatgatat caatgataag cggattaatt
      421 atgccttcaa ccatatcgct ttcggtcaga ctcagccgac cgctgaccgt gtagtttgga
      481 cattcaatca aaacgggacg tttggttata cgaaagacca gcatcttaca aaacaatcca
      541 gcttttttta ttccgacggt tatatccaaa tcgtgctgac agatgcgaga gcagtttcag
      601 atgccgataa gaaattccgt tcggcggtgg ttttgattaa cagcagcggc agggttgagg
      661 tttgtcctag aaacgatagg cgtaccgtat gccaatataa atgattgttt tagtcggttt
      721 gggaaataaa aaaatgaaga ataatgattg cttccgcctg aaaaaccccc agtctggtat
      781 ggcgctgata gaagtcttgg tcgctatgct cgttctgacc atcggtattt tggcactatt
      841 gtctgttcag ttgcggacag tcgcttccgt cagggaggca gagacgcaaa ccatcgtcag
      901 tcaaatcacg caaaacctga tggagggaat gttgatgaat ccgaccattg attcggacag
      961 caacaagaaa aactataatc tttacatggg aaaccatcat acactatcag ctgtggatag
     1021 cgattttgcg gttgatgccg tgaaaagcaa gaaggatttg gcaaaagccc agttggacag
     1081 gttcggttat gaattgaaaa atgccttgcc ggatgcggca gccatctatt acgccatctg
     1141 caaggattcg ttgggtgttg cgccgacatt gtccgccggt agtacttttt cttcaaattg
     1201 cgatgctagt gcaaatgggg atactttgat taaagtattg tgggtaaatg attcggcagg
     1261 ggattcggat atcgcccata cgaatcttga gacgaacggc aacaatatcg tatataccta
     1321 tcaggcaagg gtcggaggtc gtgaatgaaa cgtaaaatgc taaacgtacc aaaaggcaat
     1381 tatgatggta tgaagggttt taccattatt gaatttttgg ttgcgggcat gctcagtatg
     1441 attgtcctga tggcggtcgg atcgagttac ttcacatccc ggaaattaaa tgatgcggca
     1501 aacgagcgtc tttccgcgca acaggatttg cggaatgcgg caacattgat tgtccgcgat
     1561 gcgagaatgg cgggcggctt cggttgtttc aatatgtccg agcatcctgc aactgatgtt
     1621 attttcgata cgacgcaaca aaattctcct ttttccttaa aaaggaacag tacaaataaa
     1681 cttatcccca taacggaatc ttcaaatatc aattatcaga attttttcca ggttggtagc
     1741 gcattgattt ttcaatacgg aatcgatgat cttgatgcga gtgctgagac tgttgtagtc
     1801 agcagctgtt ccaaaatagc aaaactgggt aagaaaatat ctaccttgca agaagcaaag
     1861 agtgcattac agattactaa tgatgataaa caaaatggaa atatcacccg tcaaaggcat
     1921 gtggtcaatg cctatgcggt cggcaggatt gccggtgagg aaggtttgtt ccgcttccaa
     1981 ttggatgata agggcaagtg gggtaatcct cagttgctcg cgaaaaagat tagacatatg
     2041 aaagtgcagt atatctatgt ttccgactgt cctgaagatg acgatgccgg caaagaggaa
     2101 aaattcaaat atacgggtac attcgacagc tccacaaatg ctgttacgcc cgccggggtg
     2161 gaggttttat tgagtagcgg tactgatacc aagattgccg cttcttcaga caatcatatt
     2221 tatgcttacc gtatcgatgc gacaatacgc gggggaaatg tatgcgcaaa cagaacactt
     2281 tgacgggaat cccgacttct gacggacaga gggggtttgc actgtttatc gtgctgatgg
     2341 tgatgatcgt cgtggctttt ttggttgtaa ctgccgcgca gtcttacaat accgagcagc
     2401 ggatcagtgc caacgaatca gacaggaaat tggctttgtc tttggccgag gcggctttgc
     2461 gggaaggcga acttcaggtt ttggatttgg aatatgatac ggacagtaag gttacattta
     2521 gcgaaaactg tggaaaaggt ctgtgtaccg cagtgaatgt gcggacaaat aatgataatg
     2581 aagaggcttt tgacaatatc gtggtgcaag gcaagcccac cgttgaggcg gtgaagcgtt
     2641 cttgcactgc aaaatctaca ggcctgtgca ttgacaataa agggatggaa tataagaaag
     2701 gcaagggaag cgtcagcaaa ccaccacgct atattatcga atatttgggc gtgaagaacg
     2761 gagaaaatgt ttatcgggtt actgccaagg cttggggtaa gaatgccaat accttggtcg
     2821 tccttcaatc ttatgtaagc aataatgatg agtaataaaa tggaacaaaa agggtttaca
     2881 ttgattgaga tgatgatagt cgtcgcgata ctcggcatca tcagcgtcat tgccatacct
     2941 tcttatcaaa gttatattga aaaaggctat cagtcccagc tttatacgga gatggtcggt
     3001 atcaacaata ctgtcaaaca gtttattttg aaaaatcccc tggacgataa tcagaccatc
     3061 aagcgcaaac tggaaatatt tgtctcaggc tataagatga atccgaaaat tgccaaaaaa
     3121 tatagtgttt cggtaaggtt tgtcgataag gaaaaatcaa gggcatacag cttggtcggc
     3181 gttccgaagg cggggacggg ttatactttg tcggtatgga tgaacagcgt gggcgacgga
     3241 tacaaatgcc gtgataaggc ttctgccgaa gcctattcgg agaccttgtc cgcagatgcc
     3301 ggctgtgaag ccttctctaa tcgtaaaaaa taaggttgtt ttgccgatgc cgtctgaaaa
     3361 tcaatgttca gacggcattt tt
//
LOCUS       DAXHTY010000134         3375 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXHTY010000134 DAXHTY010000000
VERSION     DAXHTY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.134"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..342
                     /locus_tag="ACFNMK_001911"
     CDS             <1..342
                     /locus_tag="ACFNMK_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6365104.1"
                     /translation="LLEGIAEGTTVYADKGYDSAENRQHLEEHQLLDGIMRKACRNRP
                     LTEVQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLK
                     AANRLSAPAAA"
     gene            516..587
                     /locus_tag="ACFNMK_001912"
                     /pseudo
     CDS             516..587
                     /locus_tag="ACFNMK_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
     gene            691..1074
                     /locus_tag="ACFNMK_001913"
     CDS             691..1074
                     /locus_tag="ACFNMK_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC6365105.1"
                     /translation="MYCLRLRRLVLIFVNPLYIIATTVPAQQAFLDAAELMQWSIETE
                     GLGLNVISHKILGKDHAQVEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPA
                     MKQPCICGSGKKFKACCGKYLKPVA"
     gene            complement(1293..2087)
                     /locus_tag="ACFNMK_001914"
     CDS             complement(1293..2087)
                     /locus_tag="ACFNMK_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6365106.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSCRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            2296..3021
                     /locus_tag="ACFNMK_001915"
     CDS             2296..3021
                     /locus_tag="ACFNMK_001915"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC6365107.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLSFLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
     gene            complement(3150..>3375)
                     /locus_tag="ACFNMK_001916"
     CDS             complement(3150..>3375)
                     /locus_tag="ACFNMK_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365108.1"
                     /translation="VSGNFHFVIPAQAGIQSVEFQSFPINHRNVKFLDSRLRGNDGGE
                     GLLFRINPCSFSFLDSRLRGNDGGEGCCFG"
BASE COUNT          900 a          999 c          792 g          684 t
ORIGIN      
        1 ttgttggaag ggatagctga aggtacgacc gtctatgccg acaaaggcta cgacagtgcg
       61 gaaaaccggc aacatctgga agaacatcag ttgctggacg gcattatgcg caaagcctgc
      121 cgcaaccgtc cgctgacgga agtgcaaacc aaacgcaacc ggtatttatc gaagacccgt
      181 tatgtggtcg aacagagctt cggtacgctg caccgtaaat tccgctacgc tcgggcagcc
      241 tatttcggac tgattaaagt gagtgcgcaa agccatctga aagcgatgtg tttgaacctg
      301 ttgaaagcgg ccaacaggct aagtgcgccc gctgccgcct aaaaggcagc ccggatgcct
      361 gattatcggg tatccgggga ggattaaggg ggtatttggg taaaattagg aggtatttgg
      421 ggcgaaaaca gctgaaaacc tgtgtttggg tttcggctgt cgggagggaa aggaattttg
      481 caaaggtctc gggctatgcc ggctgctgcc gcccattgca ccttcgccga atcctgccgc
      541 ccaccgccga agccctcatg cgttcgcggt acagcgcgta tgtgctacta tagtggatta
      601 acaaaaacca gtacagcgtt gcctcgcctt agctcaaaga gaacgattct ctaaggtgct
      661 gaagcaccga gtgaatcggt tccgtactat ttgtactgtc ttcggcttcg tcgccttgtc
      721 ctgatttttg ttaatccact atacatcatc gctactaccg ttccggcgca acaggcattc
      781 ctcgatgccg ccgaactgat gcaatggagt atagaaaccg aagggctggg cttgaacgtc
      841 atctcgcaca agatactcgg caaagaccac gcccaagtcg aatttgaagc ctacttccga
      901 gacggacaac accgatccgc gcatcacgaa ctgtccggct tcgtcaacat cggcggacaa
      961 tggtatttta tcgatcccac cgttccgcat cctgcgatga aacaaccctg catttgcgga
     1021 tcaggcaaaa aattcaaagc ctgctgcggc aaatatctga aacctgtcgc ataaaaatgc
     1081 cgtctgaacg ttcagacggc attttcaacg tgcaaaaaaa aaccattcat accaagggta
     1141 agtatgaatg gtcaatacat tgcgggaaaa cgtcttactt gctgcactgc cgaaaaggga
     1201 gaaacggcag cggtaatcag cggaaatgat tgtacccgaa ttaatgttaa gaaacgttaa
     1261 tcgcgaaaat atattaacaa acctgttgaa acctattggt tttcccgtat ccacccgacc
     1321 cagcgttcaa acagcttcgg ttcgagcgcg gcaacgaccg agcgtttgaa cacgtgttca
     1381 ccactccaaa acccgtcgcc ttccaaagtc gtcagcctgc cgcccgcctc ctcgaaaatc
     1441 aacgcacccg cagcatagtc ccacagcttc tgcccaccgt gaacataaac atcataacgc
     1501 ccgcacgcca gataacacca atccaacgta ctgctgccca tactgcgtat cgtcccgaaa
     1561 ggcgcgagcg tactcatgcg gctggacagt ttgcccgaac gcagatattt gatttccaca
     1621 cccgcgattg cctcattcaa cttcttatcc acgaggcgca aaggcaggcg tgtcccatta
     1681 agaaacgcac cctgcccccg ttcggcataa aaacattcgc cgctgaccgg gttgtagatt
     1741 acgcccaatt ccgcacgccc gttgcggaca aacgccaccg acaccgcaaa atgcggcagc
     1801 ccgttgacaa aattgttcgt cccgtctatc ggatcgacaa tccacagccc cttttccccc
     1861 gaatattgtt cccacaaagc cgactgttcc tgccgcgaca tttcctcgcc caacatcggg
     1921 caatcgatca gaagcggcaa cgcggcggca aatgccgtct gcgcggcaat gtccgcctcg
     1981 ctcaacatcg aaccatcttc cttgcggcaa gacggcgtat tcaaaaaacg cggcataatt
     2041 tcggtttgcg cgatatggcg cacgactttc tgcaaacggt gtaacacttc ctactgtcct
     2101 catattttga acttgcggca cgcgaacgta tatagtggat taaatttaaa tcaggacaag
     2161 gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgct gtactggttt
     2221 aaatttaatc cactataatg tccgtttcca tcacgccgct gcgacagatt ataaccgtcc
     2281 gaaccgccaa aaactatgcc ccgattccac ctgcccgaaa acctttccgt cggacaaacc
     2341 gtcgccttgc ccgacaacat cgtccgccac ctcaacgtcc tgcgcgtccg ccccaacgaa
     2401 aacatcaccc tcttcgacgg caaaggcaag gcacacgccg cacggctgtc ttttttggaa
     2461 aaacgccgcg ccgaagccga aatcctgcac gaagacacaa ccgacaacga atccccgctc
     2521 aacattacac tgatacaatc catctcctcc ggcgatcgca tggatttcac cctgcaaaaa
     2581 agcgtcgaac tcggcgtaac cgccatacag cccgtcatca gcgaacgctg catcgtccgc
     2641 ctcgacgggg aacgcgccgc caaacgcctc gcacgctggc aggaaatcgt catctccgcg
     2701 tgcgaacaaa gcggcagaaa caccgttccc cccgtactgc ccatcatcgg ctatcgtgaa
     2761 gcactcgaca aaatgccgtc tgaaagcacc aagctgatta tgagcatcaa ccgcgcccgc
     2821 aaactcggcg acatccgcca cccatccggc gcaatcgtct ttatggtcgg gcccgaaggc
     2881 ggctggacag aacaggaaga acaacaggca tttgaagccg gctttcaggc ggttacactc
     2941 ggcaaacgga ttttacgcac agaaaccgcc ccactcgccg ccctcgccgc gatgcagacg
     3001 ctttggggcg atttcgcata aacagaaatg ccgtctgaaa cccgttcaga cggcattttg
     3061 cagccgatta agatagtagg ttcaaataag atttcccgtg tcgtcattcc cgcgaaagcg
     3121 ggaatctaga aacgaaaaac tacagggatt tatccgaaac aacaaccctc tccgccgtca
     3181 ttcccgcgca ggcgggaatc tagaaacgaa aaactacagg gatttatccg aaacaacaaa
     3241 ccctctccgc cgtcattccc gcgcaggcgg gaatctagaa atttaacgtt gcggtgattt
     3301 attggaaatg actgaaactc aacggactgg attcccgcct gcgcgggaat gacaaagtgg
     3361 aagttgcccg aaact
//
LOCUS       DAXHTY010000135         3358 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXHTY010000135 DAXHTY010000000
VERSION     DAXHTY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.135"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            500..1708
                     /locus_tag="ACFNMK_001917"
     CDS             500..1708
                     /locus_tag="ACFNMK_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC6365109.1"
                     /translation="MSEAEYFSHFISNGNGKLLEIPQRRGRQDGVFIDWLSFTLHEDS
                     LLKVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSMFRLGSEEVDYGE
                     VHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVDLALDFFDGEYTP
                     EQALLDHDNGFFDNSNMRPKSEMVGTAWRREDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNHGDMEIPLDILINQGSYFSGAFPICQKFKNMPNPERFDYRKKV
                     ANLTFQHKLRYAKNAVGKLINFMFDMGFDSDEIVRYMKADLGYPKGLEPEKYSLAGLK
                     ESLKFGFIHEQPDVDLEVELEELGIIKFKQSDKFDPDKRLFDPHHDVESERQYQLYLD
                     RMYDLHANQN"
     gene            1729..2040
                     /locus_tag="ACFNMK_001918"
     CDS             1729..2040
                     /locus_tag="ACFNMK_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365110.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSKLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            2044..2244
                     /locus_tag="ACFNMK_001919"
     CDS             2044..2244
                     /locus_tag="ACFNMK_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365111.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
     gene            2471..2701
                     /locus_tag="ACFNMK_001920"
     CDS             2471..2701
                     /locus_tag="ACFNMK_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365112.1"
                     /translation="MNIVKKYAVKAALAAGIFTPAIVMADTFDASAIGTQVANVIMGF
                     VSMVSAVGMAAITVILAIQGFKMAWSMIKSVR"
     gene            2729..3028
                     /locus_tag="ACFNMK_001921"
     CDS             2729..3028
                     /locus_tag="ACFNMK_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365113.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPKCSNYEQVKSGAYLGSMVLILFVVIYGFRLLINFLKDIGKVGA"
     gene            3147..>3358
                     /locus_tag="ACFNMK_001922"
     CDS             3147..>3358
                     /locus_tag="ACFNMK_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC6365114.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSD"
BASE COUNT         1022 a          568 c          774 g          994 t
ORIGIN      
        1 ttcagtcatt cccgataaat tgccttagca ttgaatgtct agattcccgc cttatatgat
       61 gcgctctatc aaaggggcgc attacttttc ttaacattcc cctttgacag ccaagtgaaa
      121 ggggcttttt tatgtcagta gcaaatgtaa tattttcttg ttcctattgg agaatattta
      181 aaaaatcaga ttattgcgtt ttatgtttct atcagttcag gcatggtgaa ccgcataaac
      241 tcgctgaaca agaaaatttt tcaaagcttt atcaggcgtt cgattatata gattcggttg
      301 gctcgaattt tccggtaatt atcacaacag acggttgtgg tctttcttct tgatctttaa
      361 cagtttgtca ggattgggct ttcggtcgtt gaccgttgga cgcgctttag cgcggcagac
      421 gggaaacggc tgaaagcccc ccccttgact aacagggggg ggagcgaaat aaaacaaatc
      481 cctaaaggta ctgaacaaaa tgagtgaagc agaatatttt tcccatttca tatctaacgg
      541 caatgggaag ttattagaaa ttccgcaacg taggggcagg caggacggcg tttttataga
      601 ttggttgtca ttcacactgc acgaagattc cttgctgaaa gtttccggat gccccttagt
      661 ttccgatgcc gaatatatgt ttgttttaag caaaaaattg gaggaaatat tagggtttgg
      721 catcacgagc agatgcaaat cgaagggcaa taaattttac gattcgatgt ttaggttggg
      781 atcggaagaa gttgactacg gcgaagtcca ttacggaggt cagcgaaata cggttttaat
      841 cgaattgaaa ggtgtaggtt gcaacattgc aaatccaggt tgggaattga ggcttaagca
      901 gtttttggaa gattcattga ggccgaggat aacgcgggta gatttggcac ttgatttttt
      961 tgatggggag tacacgccgg aacaggcact tttggatcac gataacggtt ttttcgataa
     1021 cagtaacatg aggccgaaat ctgaaatggt tggaacggct tggcggagag aggacgggag
     1081 cggcaagaca ttttatgtag gtcgcaagaa aaattctcgt tttgtgcgtg tttatgagaa
     1141 aggcaggcag ctaggcgata aagaaagcaa gtgggtaagg ttcgaaattc agtttaatca
     1201 tggagatatg gaaatacctt tggatattct gataaatcaa ggttcttact tttcaggcgc
     1261 tttcccgatt tgtcagaaat ttaaaaatat gccgaatccg gaaaggttcg attaccgtaa
     1321 aaaagtggct aatttaactt ttcagcataa attgagatac gcaaaaaacg cggtcggcaa
     1381 actgattaat ttcatgtttg atatgggttt tgatagtgat gaaattgtca gatatatgaa
     1441 ggcagatttg gggtatccca aagggctaga acctgaaaaa tattcgttgg ccggattgaa
     1501 ggaatctttg aaattcggct ttatccacga acaaccggat gtagatttag aggttgaatt
     1561 ggaagaactc ggaattatca aatttaagca atcagataaa ttcgatccgg ataaaaggct
     1621 tttcgatcca catcacgatg tagaaagtga gaggcaatat cagctttatc tcgacagaat
     1681 gtatgatctt catgcaaatc aaaattaacc taaaaaggaa aaattaatat gtttaatcaa
     1741 actcaaactg taacttatcc cgcaactttt ttaggagcta aaaaattcaa aggcgaaatt
     1801 gatggctcta atatcgacac ttgttccgta ttggttgcaa cacctttgcc ggcacagtcg
     1861 ggaaatgctg ttggattcac ggcagcacaa atgaagttcg gggacagtaa gaatttctca
     1921 aaattagaga atctcaaata cccgtgcgaa gttatggtaa cggttgaaat gacttcgaca
     1981 ggtaagggca tggttccttc attaattgat tttcaggtgg cagaaaagcc gaaaggttga
     2041 tttatgaaat ttgaagaacg tttcatagtt caagacttgg aaacgcatga ctttatttat
     2101 cccgatcctt tcggtgatgt ggggtttact caaaatatta aatcagcagg tcaatttgaa
     2161 agctacgaag atgcgttgaa ttcaggcata aatgaaatag gcggaggatt ccagatattt
     2221 cagttcttcg taaaatcgga ataaaagaaa aacaggctcg gcgggcggtc tgtcaacctt
     2281 tcacaaagcc cgcaaaaagg attaaaaatg aataaagcaa ctttaattat cggatttttt
     2341 gtttgcctca ttcttttcat tttctcaatt ctctatttca taaattaaaa acaaaattcg
     2401 ggagcaggct tcgcccggat ttaaacctaa gaagccgtat tactttaaat atcaaaaaag
     2461 gaaaaaaacg atgaacatcg ttaaaaaata cgctgtaaaa gcagccttgg cagccggtat
     2521 cttcacaccg gccattgtta tggcagatac ctttgatgca tccgcgattg gtacgcaagt
     2581 agcgaatgta atcatgggtt tcgtgtcaat ggtttccgcc gtgggtatgg cggccattac
     2641 cgtgattctt gcaatccaag gcttcaaaat ggcttggagc atgattaaat ctgtcagata
     2701 aacagagtga agaaaaaggg gcgtataaat gggctatcgt gtcggcataa attgttttga
     2761 tacaagattg caggcagacg actatttatt gtcgtccctt cctcctactg ttacccagga
     2821 cggaaaaatc atcaggccgg aaagggtggg cgataaatgg attttgaacg gaaagccggt
     2881 cacgctgtct tatccgaaat gttcgaatta cgaacaagtt aaatccggag cttatctcgg
     2941 gtctatggtt ttaattctgt ttgtcgttat ttacggcttc aggctgctga ttaatttctt
     3001 aaaagacata ggcaaagtag gggcgtgatg atgtttgtcg atttttggtt tttgctcgga
     3061 tttttcctgg ctttgtctgt cgctttgata tttatatgac gtgttttaaa atcaggcttt
     3121 caaaacaacc tttgaaagac agaaacatga acaagccgtt tatcacgcag gcgcagttgg
     3181 cactttataa atatcagccg tccagcaagt attttgggca gtcgatggca ttaattgcgt
     3241 ctaaggaatt tgaagagttt gtaagaaatg taaaagaata cgacgtaata gaatgtttct
     3301 cttatttttt aaataagagg gtaacgcata atatttggaa aatttatttt tctgatga
//
LOCUS       DAXHTY010000136         3332 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXHTY010000136 DAXHTY010000000
VERSION     DAXHTY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.136"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            153..605
                     /gene="dut"
                     /locus_tag="ACFNMK_001923"
     CDS             153..605
                     /gene="dut"
                     /locus_tag="ACFNMK_001923"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC6365115.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVVVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            681..1868
                     /gene="dapC"
                     /locus_tag="ACFNMK_001924"
     CDS             681..1868
                     /gene="dapC"
                     /locus_tag="ACFNMK_001924"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC6365116.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            2168..2947
                     /gene="yaaA"
                     /locus_tag="ACFNMK_001925"
     CDS             2168..2947
                     /gene="yaaA"
                     /locus_tag="ACFNMK_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC6365117.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGQYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3030..3120
                     /locus_tag="ACFNMK_001926"
     tRNA            3030..3120
                     /locus_tag="ACFNMK_001926"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3064..3066,aa:Ser,seq:tga)
     gene            3198..3287
                     /locus_tag="ACFNMK_001927"
     tRNA            3198..3287
                     /locus_tag="ACFNMK_001927"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3232..3234,aa:Ser,seq:cga)
BASE COUNT          877 a          778 c          822 g          855 t
ORIGIN      
        1 gtgtgcataa tgttcgtctt cataacggga agggcgggca ttatacactg gcaacggatt
       61 tcaaaacaaa accgatttgc cgtgtttcag cgtaaacacg gcttgtgtat aatctcccat
      121 ctttgaaatc ggccgtatgc aggagcaaga cgatgaatat tgaagtagaa atgaaagtat
      181 tggacgagag gatggcggat tttatccctg cctatgcaac ggagggttct gcaggtttag
      241 atttgcgcgc ctgtttggat gaggaagtcg ttttgcagcc gggtgaaacg tttcttgtgc
      301 cgacgggttt ggcaatttat ttggcgaatc ccgcatatgc cgccgttttg ctgccccgtt
      361 ccggcttggg gcataaacac ggcattgttt tgggcaattt ggtcggtttg attgactccg
      421 attatcaagg ggaattgaag gtgtcgttat ggaacagaag cagcgaaccg tttactgtca
      481 aaccgtttga gcgtatcgcg cagatggttg tcgtgccaat cgtgcaggcg cgtttcaaac
      541 gtgtcgagga atttgtcgga agcagccggg gtgagggcgg cttcggcagt acgggtttgc
      601 attgaatata aaatgccgtc tgaagggcac gtcaggttca gacggcatat cttccgatat
      661 accgacaacc ggagaaaatc atgaatacct tactcaacca actcaaaccc tatccctttg
      721 cccgactgcg tgaagcgatg cagggcattt ccgcgcccga aggtctggaa gccgtccccc
      781 ttcacatcgg cgaaccgaaa catccgacac cgaaagtcat tacggatgcg ctgaccgcct
      841 cattgcacga gttggaaaaa tatccgctga cggccggtct gcctgaactg cgtcaggcgt
      901 gtgcaaactg gttaaaacgc cgttacgatg gcttgacagt ggatgcggat aatgaaattc
      961 tgccggtttt aggcagtagg gaggcgttgt tttcttttgt tcaaaccgtg ttgaaccctg
     1021 tttcagacgg catcaaaccc gcaattgtca gcccgaatcc cttttatcag atttacgaag
     1081 gtgcgacact tttgggcggc ggtgaaatcc attttgccaa ttgccccgcg ccgtctttca
     1141 accccgattg gcgtagtatt tccgaagagg tttggaaacg caccaaactg gtgttcgtct
     1201 gctcgcccaa caaccccagc ggcagcgtac tggatttgga tggatggaaa gaagtttttg
     1261 atttacagga taaatatggt ttcattattg cctcggatga atgctattcc gaaatctatt
     1321 tcgacggcaa caaaccttta ggctgcctgc aggccgctgc acagttgggt cgaagcaggc
     1381 aaaaactgct tatgttcacc agtttgtcca agcgttccaa cgttccgggc ctgcgttccg
     1441 gttttgtcgc cggcgatgcc gaactgctta aaaactttct gctttacaga acctatcacg
     1501 gcagtgcaat gagcattccc gtgcagcgcg caagtattgc cgcttgggat gacgaacagc
     1561 acgttatcga caaccgccgc ctgtatcagg aaaaatttga gcgcgttatt cccattttgc
     1621 aacaggtatt tgatgttaaa ttaccggatg cctcgtttta catctggttg aaagtccccg
     1681 atggcgacga tttggcattt gcacgcaatt tatggcaaaa agccgctatc caagtattgc
     1741 ccggacgctt tttggcacgg gataccgaac agggcaatcc cggggaaggt tatgtccgta
     1801 tcgctttggt tgccgatgtc gcaacttgtg tcaaagctgc ggaaaccatt gtttccctat
     1861 atcggtaaag aataaaaaaa tgccgtctga acttttgttc agacggcatt tttcaatatt
     1921 ttacggttga atttgctata acggtattta tagtggatta acaaaaatca ggacaaggcg
     1981 acgaagccga agacagtaca gatagtacgg caaggcgacg aagccgaaga cagtacagat
     2041 agtacggcaa ggcgaggcaa cgctgtactg gtttaaattt aatccactat acttcacttt
     2101 taatcggctt gcccgcaaac acgtttaaac ttaaaatccc cgtgtttgac acaataccga
     2161 gcagattatg ttttttgtcc tttcccctgc gaagaacctt aatgaaaaag accctgcccc
     2221 tgtcagcgag tttacccaac ccgacctgct ggcagagtcc gacattctaa tgcagcagtt
     2281 gcgcgagctt gcgccgcaac agattgccga actgatgcac gtttccgaca aaattgccct
     2341 cttaaacgcg cagcgcaatg cagaatggaa cacgccgttt acgccggaaa acgccaaaca
     2401 ggcggtcttt atgttcaacg gcgatgttta cgaaggtatg gatgcaaaca cattggatat
     2461 tggacagata cgctatctgc aaaaccatgt ccgcctgctg tccggtctgt acggtcttct
     2521 tcgcccgtta gacctgatac agccctatcg tttggaaatg gggacggcat ttgccaattt
     2581 gcgcggcaag aatttgtatg agttttgggg cgacatcatt accaaccttt taaatgacac
     2641 gcttgcccaa gcaggcagca atacgcttgt caaccttgcc tcacaggaat atttcaagtc
     2701 cgtcaacacg aaaaaacttc gggcgcggct gattaccccg atatttaaag acgaaaaaaa
     2761 cggtcaatat aaaatcatca gtttctatgc caagcgcgcg cgcggattaa tggtgcgcta
     2821 tgcggcagaa catcatatta ccgaccctga aatgctgaaa aattttaatt acgaaggcta
     2881 cgcattcaat gacgcggctt caaatgaaag cgaatgggtt tttatgcgtt cggaacaaat
     2941 aaagtgaaaa caataaatta agtattttcc gaaaaaagtg cttggcaaaa tgtataaatt
     3001 tcattattat tcctaatctt caagaagacg gaagcgtggc agagcggttt aatgcaacgg
     3061 tcttgaaaac cgtcgagggt tgatagccct ccgtgagttc gaatctcacc gcttccgcca
     3121 atccttgagg tcaaaaacca aataaaaatg aagtagcata aagcatcggc atttttattt
     3181 ggttttactg cattatcgga aacgtggcag agaggctgaa tgcagcggac tcgaaatccg
     3241 ctgagggtgc aaatcctccg tgggttcgaa tcccaccgtt tccgccacaa aacaaaaccg
     3301 ccctgattcg gggcggttct tttttgttca ag
//
LOCUS       DAXHTY010000137         3326 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXHTY010000137 DAXHTY010000000
VERSION     DAXHTY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.137"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            34..1206
                     /gene="ctrA"
                     /locus_tag="ACFNMK_001928"
     CDS             34..1206
                     /gene="ctrA"
                     /locus_tag="ACFNMK_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HFC6365118.1"
                     /translation="MEVKKISSFIICGLVLITLTACGAIPSSGPSAKKIVSLGQQSEA
                     QIPEVELIDVNHAVAQSLYKAQMNQSFTQFGDGYASTGTLNIGDVLDIMIWEAPPAVL
                     FGGGLSSMGSGSAQQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKKM
                     ANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNVQ
                     LTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSARG
                     LSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVYR
                     VNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            1221..2384
                     /gene="ctrB"
                     /locus_tag="ACFNMK_001929"
     CDS             1221..2384
                     /gene="ctrB"
                     /locus_tag="ACFNMK_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HFC6365119.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDVFSRFNGFGFRGEEEAFYQYYKDKLNINFDAISGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARSDTVRYAEEVVKTAEERVKETSQN
                     LTNYRISNGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     KSLRKEINQQLRAISGGGQSSLSNQAADYQRVYLENQLAEQQLAAAMTSLESAKAEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            2384..3181
                     /locus_tag="ACFNMK_001930"
     CDS             2384..3181
                     /locus_tag="ACFNMK_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6365120.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMMMMWRNASNRSIGAISANASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            3178..>3326
                     /locus_tag="ACFNMK_001931"
     CDS             3178..>3326
                     /locus_tag="ACFNMK_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6365121.1"
                     /translation="MISVEHVSKQYQMRGGMRTVLDDINFSLQKGEKVGILGRNGAGK
                     STLIR"
BASE COUNT          817 a          608 c          884 g         1017 t
ORIGIN      
        1 caaaaacatt attttagttg attaggttat attatggaag taaaaaaaat atcttctttt
       61 atcatctgcg gtttggtact tattacgctg acggcatgtg gagccattcc ttcctcaggt
      121 ccaagtgcta aaaagattgt ctccttaggg caacagtccg aagctcaaat tcctgaagtg
      181 gagttgattg acgtcaatca tgctgtggcg cagtctttgt ataaagctca gatgaatcag
      241 tcttttaccc agtttggcga tggttatgct tctaccggta cgctaaatat tggtgatgta
      301 ttggatatta tgatttggga agcgccgccg gcggtattgt ttggcggtgg gctttcttcg
      361 atgggctcgg gtagtgcaca gcaaaccaag ttgccggagc agttggtcac ggcacgtggt
      421 acggtttctg tgccgtttgt tggcgatatt tcggtggtcg gtaaaacgcc tggtcaggtt
      481 caggaaatta ttaaaggccg cctgaaaaaa atggccaatc agccgcaagt gatggtgcgc
      541 ttggtgcaga ataatgcggc aaatgtatcg gtgattcgcg caggcaatag tgtgcgtatg
      601 ccgttgacgg cagccggtga gcgtgtgttg gatgcggtgg ctgcggtagg tggttcaacg
      661 gcaaatgtgc aggatacgaa tgtgcagctg acacgtggca atgtagtacg aactgttgcc
      721 ttggaagatt tagttgcaaa tccgcgacaa aatattttgc tgcgtcgcgg tgatgtggtt
      781 accatgatta ccaatcccta tacctttacg tctatgggtg cggtggggag aacacaagaa
      841 atcggttttt cagccagagg cttatcgctt tctgaagcca ttggccgtat gggcggtttg
      901 caagaccgcc gttctgatgc gcgtggtgtg tttgtgttcc gctatacgcc attggtggaa
      961 ttgccggcag aacgtcagga taaatggatt gctcaaggtt atggcagtga ggcagagatt
     1021 ccaacggtat atcgtgtgaa tatggctgat gcgcattcgc tattttctat gcagcgcttt
     1081 cctgtgaaga ataaagatgt attgtatgtg tcgaatgcgc cgttggctga agtgcagaaa
     1141 ttcttgtcgt ttgtgttctc gccggttacc agtggcgcga acagtattaa taatttaact
     1201 aattaatgtg agtaattaag atgtctgagc aacttcctgt ggcagttgcc actgaaacca
     1261 aagccgagcg taaaaagccg aaaaagaaaa gttggattaa aaagctaagc cctttatttt
     1321 gggtaacggt gattatccct acggtaattt cgttggtgta tttcggcttc ttcgcttccg
     1381 atcgttttac gtcgcaatcg agctttgtgg tgcgctcgcc taaaagccaa tcttctctca
     1441 atggcctggg tgccattttg cagggcacag gttttgcccg tgcgcaagat gatatttaca
     1501 cggttgggga gtatatgcgt tcgcgctcgt ctttggatga actgcgtaaa atcttgccgg
     1561 tgcgtgaatt ttatgaaact aagggcgatg tatttagccg atttaatggt tttggattcc
     1621 gtggtgagga agaggctttt tatcaatact ataaagataa gctgaacatt aattttgatg
     1681 cgatttcagg tatctctacg cttaacgtta catcatttga tgcgcttgaa tctaagaaaa
     1741 tcaatgaagc tttgttaaaa cagggcgaag cattgattaa ccagctgaat gatcgtgcgc
     1801 gctctgatac agtgcgttat gcggaagaag tggtgaaaac tgctgaagag cgtgtgaaag
     1861 aaacttcaca gaatctgact aattaccgca tttctaatgg tgtttttgac ttgaaagcgc
     1921 aatcagaggt acagatgggc ttggtatcca agctgcaaga tgagttgatt gtgattcaaa
     1981 cccagcttga tcaggtgaaa gcagttacgc cagaaaaccc gcaaattcca ggtttgcagg
     2041 cacgtgagaa gagcttacgc aaagaaatta accaacagtt acgtgccatt tcgggtggtg
     2101 ggcaatcatc attatcaaat caagcagctg attatcagcg cgtttatttg gaaaatcaat
     2161 tagctgagca gcaattggca gcggccatga cttctctgga aagcgctaaa gctgaagctg
     2221 atcgccagca gctttattta gaagtgattt ctcagccgag cctgccggat ttggcgcatg
     2281 aacctaaacg gttatacaac attgttgcca ctctgattat cggcttgatg gtttatggta
     2341 ttttgagcct gttgactgcc agcattcgtg agcataaaaa ctgatgaaag ccttgcataa
     2401 aacatcattt tgggaatctt tggctattca aaggcgcgtg atcggcgcgc ttttgatgcg
     2461 ggaaattatc acccgttacg gtcgcaataa tattggcttt ttatggctgt ttgttgaacc
     2521 gttgctgatg acattcgtta ttgtcttgat gtggaaattt ttaagggcag accgatattc
     2581 aactttgaat attgtggcgt ttgcgattac cggttatcca atgatgatga tgtggcgtaa
     2641 tgcctctaat cgatcaattg gtgccatttc tgctaatgcc agcctgcttt atcaccgcaa
     2701 tgtaagagtt ttggatacca ttttggcgcg catgattttg gaaattgctg gtgcaaccat
     2761 tgcgcagatt gtgattatgg cggtattgat tgcgattggc tggattgaaa tgccggcaga
     2821 tatgttttat atgctgatgg cttggctttt gatggctttt tttgcgattg gtttgggttt
     2881 ggtgatttgt tcgattgcct ttaatttcga gccgtttggc aagatttggg gcacattgac
     2941 ttttgtgatg atgccgttat ccggtgcgtt cttttttgtg cataatttgc cgcccaaggt
     3001 acaagaatat gcattaatga ttccgatggt gcatggcaca gaaatgttcc gtgccggata
     3061 ttttggcagc gatgtaatta cctatgaaaa tccttggtat atcgtattgt gcaatctggt
     3121 gttgttgttg tttggcttgg cgatggtcag taaattcagt aaaggagtcg agccgcaatg
     3181 atttcggttg aacatgtttc caagcagtat caaatgcgcg gcggtatgcg gacggtgtta
     3241 gacgatatca atttttcgct gcaaaagggt gagaaagttg gtattttggg tcgaaacggt
     3301 gcaggtaaat cgacgttgat ccgttt
//
LOCUS       DAXHTY010000138         3190 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXHTY010000138 DAXHTY010000000
VERSION     DAXHTY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.138"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            112..2880
                     /gene="tdfH"
                     /locus_tag="ACFNMK_001932"
     CDS             112..2880
                     /gene="tdfH"
                     /locus_tag="ACFNMK_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC6365122.1"
                     /translation="MKSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGGLKFNSNSGKWER
                     DFQRPYWKTKWYQKYNDPQELQKYIEGHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYREHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEEVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
     gene            2910..3149
                     /locus_tag="ACFNMK_001933"
     CDS             2910..3149
                     /locus_tag="ACFNMK_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365123.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VDFGYFWLFRVIYESSFPQKRESRHSMLRQFIEND"
BASE COUNT          901 a          793 c          775 g          721 t
ORIGIN      
        1 caattgaaat cttcagacgg tatatcgaat ttacactttt taatgtttat gccgcctaaa
       61 aaaatgctaa tatatttctt aattgtctga ctgtttattg ttgaggaaaa tatgaaatct
      121 tctttccggt tgaagccgat ttgtttttat cttatgggtg ttatgctata tcatcatagt
      181 tatgccgaag atgcagggcg cgcgggcagc gaggcgcaga tacaggtttt ggaagatgtg
      241 cacgtcaagg cgaagcgcgt accgaaagac aaaaaagtgt ttaccgatgc gcgtgccgta
      301 tcgacccgtc aggatatatt caaatccagc gaaaacctcg acaacatcgt acgcagcatc
      361 cccggtgcgt ttacacagca agataaaagc tcgggcattg tgtctttgaa tattcgcggc
      421 gacagcgggt tcgggcgggt caatacgatg gtggacggca tcacgcagac cttttattcg
      481 acttctaccg atgcgggcag ggcaggcggt tcatctcaat tcggtgcatc tgtcgacagc
      541 aattttattg ccggactgga tgtcgtcaaa ggcagcttca gcggctcggc aggcatcaac
      601 agccttgccg gttcggcgaa tctgcggact ttaggcgtgg atgacgtcgt tcagggcaat
      661 aatacctacg gcctgctgct aaaaggtctg accggcacca attcaaccaa aggtaatgcg
      721 atggcggcga taggtgcgcg caaatggctg gaaagcggag catctgtcgg tgtgctttac
      781 gggcacagca ggcgcagcgt ggcgcaaaat taccgcgtgg gcggcggcgg gcagcacatc
      841 ggaaattttg gcgcggaata tctggaacgg cgcaagcagc gatattttgt acaagaaggc
      901 gggttgaaat tcaattccaa cagcggaaaa tgggagcggg atttccaaag gccgtactgg
      961 aaaaccaagt ggtatcaaaa atacaatgac ccccaagaac tgcaaaaata catcgaaggt
     1021 catgacaaaa gctggcggga aaacctggcg ccgcaatacg acatcacccc catcgatccg
     1081 tccagcctga agcagcagtc ggcaggtaat ctgtttaaat tggaatacga cggcgtattc
     1141 aataaataca cggcgcaatt tcgcgattta aacaccaaaa tcggcagccg caaaatcatc
     1201 aaccgcaatt atcaattcaa ttacggtttg tctttgaacc cgtataccaa cctcaatctg
     1261 accgcagcct acaattcggg caggcagaaa tatccgaaag ggtcgaagtt tacaggctgg
     1321 gggcttttaa aggattttga aacctacaac aacgcaaaaa tcctcgacct caacaacacc
     1381 gccaccttcc ggctgccccg cgaaaccgag ttgcaaacca ctttgggctt caattatttc
     1441 cacaacgaat acggcaaaaa ccgctttcct gaagaattgg ggctgttttt cgacggtcct
     1501 gatcaggaca acgggcttta ttcctatttg gggcggttta agggcgataa agggctgctg
     1561 ccccaaaaat caaccattgt ccaaccggcc ggcagccaat atttcaacac gttctacttc
     1621 gatgccgcgc tcaaaaaaga catttaccgc ttaaactaca gcaccaatac cgtcggctac
     1681 cgtttcggcg gcgaatatac gggctattac agctcggaag gcgaatttaa gcgggcattc
     1741 ggagaaaact cgccgacata cagggaacat tgcaacccga gctgcgggct ttatgaaccc
     1801 gtattgaaaa aatacggcaa aaagcgcgcc aacaaccatt cggtcagcat tagtgcggac
     1861 ttcggcgatt atttcatgcc gttcgccagc tattcgcgca cacaccgtat gcccaacatc
     1921 caagaaatgt atttttccca aatcggcgac tccggcgttc acaccgcctt aaaaccagag
     1981 cgcgcaaaca cttggcaatt tggcttcaat acctataaaa aaggactgtt aaaacaagat
     2041 gatacattag gattaaaact ggtcggctac cgcagccgca tcgacaacta catccacaac
     2101 gtttacggga aatggtggga tttgaacggg gatattccga gctgggtcag cagcaccggg
     2161 cttgcctaca ccatccaaca tcgcaatttc aaagacaaag tgcacaaaca cggttttgag
     2221 ttggagctga attacgatta tgggcgtttt ttcaccaacc tttcttacgc ctatcaaaaa
     2281 agcacgcaac cgaccaactt cagcgatgcg agcgaatcgc ccaacaatgc gtccaaagaa
     2341 gaccaactca aacaaggtta tgggttgagc agggtttccg ccctgccgcg agattacgga
     2401 cgtttggaag tcggtacgcg ctggttgggc aacaaactga ctttgggcgg cgcgatgcgc
     2461 tatttcggca agagcatccg cgcgacggct gaagaacgct atatcgacgg caccaacggg
     2521 ggaaatacca gcaatgtccg gcaactgggc aagcgttcca tcaaacaaac cgaaaccctt
     2581 gcccgccagc ctttgatttt tgatttttac gccgcttacg agccgaagaa aaaccttatt
     2641 ttccgcgccg aagtcaaaaa tctgttcgac aggcgttata tcgatccgct cgatgcgggc
     2701 aatgatgcgg caacgcagcg ttattacagt tcgttcgacc cgaaagacaa ggacgaagaa
     2761 gtaacgtgta atgctgataa aacgttgtgc aacggcaaat acggcggcac aagcaaaagc
     2821 gtattgacca attttgcacg cggacgcacc tttttgatga cgatgagcta caagttttaa
     2881 aggcagcccg cgttttgtag aaaaccgcaa tgccgtctga aagcccttca gacggcattt
     2941 gtttccccaa acgcatcatc ctgccgcaag cctatgccaa tccgttttat cgcatcggca
     3001 actcaaagaa aaatccattt cattcccacg cagggaagcc ggttgatttc ggttattttt
     3061 ggttgtttcg ggtaatttat gagtcgtcat tcccgcaaaa gcgggaatct agacattcaa
     3121 tgctaaggca atttatcgaa aatgactgaa actcaaaaaa ctggattccc actttcgtgg
     3181 gaatgacgcg
//
LOCUS       DAXHTY010000139         3165 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXHTY010000139 DAXHTY010000000
VERSION     DAXHTY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.139"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(255..1769)
                     /gene="katA"
                     /locus_tag="ACFNMK_001934"
     CDS             complement(255..1769)
                     /gene="katA"
                     /locus_tag="ACFNMK_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC6365124.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTYDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDEQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCHRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            2117..2968
                     /locus_tag="ACFNMK_001935"
     CDS             2117..2968
                     /locus_tag="ACFNMK_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6365125.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDARFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALQKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESLEIPAA
                     HKRKTAE"
BASE COUNT          709 a          813 c          883 g          760 t
ORIGIN      
        1 ggaaaagttt tcgtgccggt ttgaaccggt ggggatggtg cgggaatgac ggcggaaaag
       61 tgggctgtgg ggcgggaatg acgggatatt ttgcgtttaa taaaaaacgc ccactgaaac
      121 ggcgggggtg gggtgtggaa atgccgtctg aagcggtcaa acgacgtttc agacggcatt
      181 tttatgcccg gttatttccg gtagcggacg gcgcgggaca ggatttcttc aatttccatc
      241 cacataatgc ccccttacag caaaccgggc tggccgagtg cggggtcggt cgcgcgggcg
      301 gcttgtgcgt cttcgacagt cagtccgagg gcttttgcca cgccttcgcc gtatgccggg
      361 tcgcaacggt ggcagttgcg gatatggcgg tatttgatga agtcgggcgc atcgcccatg
      421 gctgcggcgg tgttgccgaa caaagcctgt ttctgctcat cgttcatcag gttgaacagg
      481 gcgcgcggtt ggctgaaata gtcgtcatcg tcttggcggt agtcccagtg cgccgcgtcg
      541 ccgttgattt tcaaaggcgg ttcggcgaag tcgggttgtt gctgccattg gccgaagctg
      601 ttgggttcgt agtgcggcag gctgccgtag ttaccgtcgg cgcggccttg gccgtcgcgc
      661 tggttgctgt gaacagggca acgcggacgg ttgacgggga tttggcggaa gttcacgccc
      721 aaacggtagc gttgcgcatc ggcgtaattg aacaaacggg cttgcagcat tttatctggg
      781 ctcgcgccga taccgggaac gaggttgctc ggtgcgaagg cggattgttc cacatcggcg
      841 aagaagtttt cgggattgcg gttcaactcg aattcgccca cttcaatcag cggatagtct
      901 tttttcggcc aaactttggt caaatcaaac ggatgatagg gtactttttc cgcatcggct
      961 tcaggcatga cttggatgta catcgtccat ttcggaaact cgccgcgctc gatggcttcg
     1021 tacaggtcgc gttgatggct ttcgcggtcg tcggcgatga ttttggcggc ttcttcgttg
     1081 gtcaggtttt taatgccttg ctggctgcgg aaatgaaatt tcacccagaa acgctcgcct
     1141 gcttcgttcc agaagctgta agtatgcgaa ccgaagccgt gcatatggcg gtagctggcg
     1201 ggaataccgc ggtcgctcat cacgatggta acttggtgca acgcttcagg cagcagcgtc
     1261 cagaagtccc agttgtttgt ggcggaacgc atattggtgc gcggatcgcg tttgacggct
     1321 ttgttcaggt cagggaattt acgcgggtcg cgcaggaaga acacgggcgt gttgttgccg
     1381 accacatccc agttgccttc ttcggtataa aatttcaagg caaaaccgcg gatgtcgcgt
     1441 tctgcatcgg ctgcgccgcg ttcgcctgcc acggtggtga aacgggcgaa catctcggtt
     1501 tttttgccga cttcgctgaa gattttggcg cgggtgtatt tggtgatgtc gtacgttacg
     1561 gtaaacgtac cgaacgcgcc cgaacctttg gcgtgcatac ggcgttcggg gatgacttcg
     1621 cgcacgaagt cggcgagttt ttcattcagc cacaaatcct gcgccagcag agggccgcga
     1681 ggaccggcgg tcaggctgtt ttgattgtcg gcaacaggcg cgccgttgtt catggtcaga
     1741 tgggttacag ggcatttgga ggtagtcatc gctcttgttc cttttctcag gttggtcaaa
     1801 tgggggtaaa cggcttacag tacgatttgg cggaaagcgt attcgtaacc ggtttcttga
     1861 ttgcaataaa tttcttgaat cgacatttta tttccctttt gtaaaaacta tggatgcgac
     1921 tatacgccaa gattttcgct attaaaacta tgaaatcgat ttaatattat tataagcaat
     1981 cggtttttga ttttcgtttg ttttttgtta tcagaaagaa tccgaacccg ctcattaaaa
     2041 ccatttataa tgcaatgacg ctttgcggca ttttttgcgc cgacaggctg aaaataacaa
     2101 ttttccccac attatcatga ccttactcgg aataaagctc aaacagaccc agcagctcaa
     2161 ccagcggctg caacaatctt tgcgcgtatt gcagatgtcg ggtatcgaac ttgaacgcga
     2221 ggtcgaaaac tggctgtcgg acaatcccct gctcgaacgc aaagacacgg atgaattttc
     2281 cgatgctagg ttttcccatt acaccgcgcc tgcccgtcaa atcggcggag acgaaggcga
     2341 agatatgctg tccaacatcg ccggtgagca ggatttcaag caatacctgc acgcgcaagt
     2401 atgcgaacac ccgctttccg accaagaagc cgcctgtgtc cacatcctta tcgatttcct
     2461 tgacgagcag ggttatctga ccgacagcat cgaagacatc ctcgaccata cgcccttgga
     2521 gtggatgttg gatgaagcaa tgctgaaaca agccctgact gcattgcaaa aattcgaccc
     2581 ggcaggcgtg gccgccgccg atttgaacga atcgctgata ctgcagatag aaagattggg
     2641 cgaatgtgct gccaaacccg ccgccctgca tatcgtccga aacgccctcg acagcattga
     2701 cggcaaccgc agccaaaccc tcgcacgaat aaaaaaacac ctgccccaaa ccgacagcgg
     2761 cacactcgaa gccgcactcg acctcattgc ttcgctcaat ccctttcccg ccgccggttt
     2821 tgcctcgtcc acacccacgc cgtattctga cgaggcactc gccaacctgc tggctttccg
     2881 cggcatggag gtttctcgcc gcaccattgc caaatacaga gaatcccttg agattccggc
     2941 agcacacaaa cgcaaaaccg cagaataatt gctgaataat cttataaaga caacaaacca
     3001 aaagccggca tttctgcgaa agcgggaatg ccgaatccgt ccgcgcggaa acctgcatcc
     3061 cgtcattccc gcgaaagagg gaatctagaa acgcaaagct gcaagaattt atcggaaatg
     3121 accgaaactc aacgaacctg gattcccgtt ttcgcgggaa tgccg
//
LOCUS       DAXHTY010000140         3111 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXHTY010000140 DAXHTY010000000
VERSION     DAXHTY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.140"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            271..894
                     /locus_tag="ACFNMK_001936"
     CDS             271..894
                     /locus_tag="ACFNMK_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC6365126.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1005..2387
                     /locus_tag="ACFNMK_001937"
     CDS             1005..2387
                     /locus_tag="ACFNMK_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6365127.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYAGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2391..2915
                     /locus_tag="ACFNMK_001938"
     CDS             2391..2915
                     /locus_tag="ACFNMK_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC6365128.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          629 a          840 c          936 g          706 t
ORIGIN      
        1 ctgctgccgc ccgaaaagcg tgatgcgctg atggtgctgc tgcataatcc ggacgacgga
       61 taaccggtga aaacggaaaa tgcccgggtg gtgttgcctg tgcctgtttg gacaagcgtt
      121 cttgagggcg gtaaaatcaa ggtttgcccg aaaagggcag ggcgatatgc cgcgcttgga
      181 ttttgtgcgc ggtttttcgc gttggaagca atcattgtct gtttgaatat ttaatgccgt
      241 ctgaaacgtc cggacgcgcc cccgttccct atgaaaaaac cgaccgatac cctacccgtt
      301 aatctgcaac gccgccgcct gttgtgtgcc gccggtgcgt tgttgctcag tcctctggcg
      361 cacgccggcg cgcaacgtga ggaaacgctt gccgacgatg tggcttccgt gatgaggagt
      421 tctgtcggca gcgtcaatcc gccgaggctg gtgtttgaca atccgaaaga gggcgaacgt
      481 tggttgtctg ccatgtcggc acgtttggca aggttcgtcc ccgaggagga ggagcggcgc
      541 aggctgctgg tcaatatcca gtacgaaagc agccgggccg gtttggatac gcagattgtg
      601 ttggggctga ttgaggtgga aagcgcgttc cgccagtatg caatcagcgg tgtcggcgcg
      661 cgcggcctga tgcaggttat gccgttttgg aaaaactaca tcggcaaacc ggcgcacaac
      721 ctgttcgaca tccgcaccaa cctgcgttac ggctgtacca tcctgcgcca ttaccggaat
      781 cttgaaaaag gcaacatcgt ccgcgcgctt gcccgcttta acggcagctt gggcagcaat
      841 aaatatccga acgccgtttt gggcgcgtgg cgcaaccgct ggcagtggcg ttgattttga
      901 acccgcgccg caaccgaaat acggcgaatc ctgtataatc cgaaaatctg ttcactggaa
      961 gttcagacgg cattgcaact gttaatgccg tctgaaaaaa tatgatggca agagacaacc
     1021 gcatccaaat gtttccgcac gaatggcgcg ccagtacgac gctttccggc gtgtacgcgc
     1081 tgcgtatgct gggtatgttc ctcgtgctgc ccgtattggc ggtgtatgcc gcctcgctgc
     1141 ccggcgcgga aggcaacaaa acgctggtcg ggctggcaat gggcatttac gggctgacac
     1201 aggctctgct gcaactgcct ttgggcatcg cttccgacaa gttcgggcgc aagaaaacca
     1261 tttatgcggg actggtcgtg tttgcggcgg gcagctttct tgccgccgcc gccgatacgc
     1321 tgcccatgct ggtcgccgca cgcgccatac agggtgcagg ggcggtcagt gcggcggtta
     1381 ccgcgctgct ggcggatttg acgcgcgacg gcgtacgtac ccgcgcaatg gcgatgatcg
     1441 gtttgagtat cggtttgacg ttttcggtca gcctcgtcgt tgcccccgtg attgccgacg
     1501 cggtcggcgt tcgcggactg tttatgctga ccggcattct gaccgtcatc agcatcggcg
     1561 tggtggcgtg gatgactccc gatcccgaag tttccaagct gcacgaagat acgcaggcgc
     1621 agccttcgcg cataggcgaa gttttgaaaa accgtaggct gctgacgctt gatttcggca
     1681 ttttcgccct gcacgccgca caaatggcat tgtttaccgc gctgcctttc gcgatgaccc
     1741 agctcggttt ggaaaaaata cagcattgga aagtctatct gccttcgacc attacgggct
     1801 tggtggtgat ggttccgctg attatcgtcg gcgagacgcg caacaagctt aagcaggttt
     1861 ttgttttggg tatcgtctgt attgcggcgg cgcagctcgg tttgctgtcc ggtatgcgct
     1921 cggtaggctt gattaccgct tatttggttg tttactttat cggttttaat gtgttggaag
     1981 cgagcctgcc gtctatggtt tccaaaatcg cgccgtccga cctgaagggt acggcgatgg
     2041 gcgtgtacaa cacgatgcag tcgctcggac tgtttgccgg cggcgcggca ggcggtttgc
     2101 tgtttcaaaa atacggcttt tccggcgtgt ttgccttttg cagtatattg atgctgctgt
     2161 ggctggtaat tgccgtttta tcgcctgcgc ccaagcccgt caaaaacctc agttaccctg
     2221 tcggcggcgt gtggcagggc aatcaggaag ggttataccg cgccttgtcg gagcttgagg
     2281 gtgtggaaga catcggtttc agtttcgacg ggcagaccgt ctatctcaaa gtgttgcaga
     2341 agggtttcga tcaggctgcc gctgaaaaaa tcatcacagg agtttaaaaa atgtcattga
     2401 acaaagtcat cctcatcggc cgcctcggac gcgatcccga agtgcgctat atgcccaacg
     2461 gcgaggcggt ttgtaatttc agcgtcgcca ccagcgaaac ttggaacgac cgcaacggcc
     2521 aacgtgtaga gcgtactgag tggcacaaca tcaccatgta ccgcaaactg gcggaaattg
     2581 ccgggcaata cctcaaaaaa ggcgggctgg tttatctgga aggcagaatc caaagccgca
     2641 aataccaagg caaagacggc atcgaacgca ccgcttacga tattgtcgcc aacgaaatga
     2701 aaatgttggg cgggcgcaat gaaaacagcg gcggtgcgcc ttacgaggaa ggttacggtc
     2761 agagtcagga ggcttaccaa cgccccgcgc agcaaagccg gcagcccgcc tccgacgcgc
     2821 cgtcccatcc ccaagaagca ccagccgcgc cgcgccgcca acccgtgcct gccgccgccc
     2881 cggtcgagga cattgacgac gatatcccgt tctgaatttt acggccggac atcctcgcgg
     2941 agggaaatca taaaggacgg agaaacctta aaccttacgg ggcagggttt ctcttttttg
     3001 catttgggcc ggcttgttgc cgccctttgc gttgggcgga acgttgcgat tgcgccgttc
     3061 gggcgttttg aggcagtgat gcctgccgca cgtccccgct gttttcagac g
//
LOCUS       DAXHTY010000141         3063 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXHTY010000141 DAXHTY010000000
VERSION     DAXHTY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.141"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(255..731)
                     /gene="greA"
                     /locus_tag="ACFNMK_001939"
     CDS             complement(255..731)
                     /gene="greA"
                     /locus_tag="ACFNMK_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC6365129.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1123..2424
                     /gene="aroA"
                     /locus_tag="ACFNMK_001940"
     CDS             1123..2424
                     /gene="aroA"
                     /locus_tag="ACFNMK_001940"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC6365130.1"
                     /translation="MTESVRLPAASLKLSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGTDVEYLGNEHYPPLHIGERQDNGE
                     HVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EDYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2467..3000)
                     /locus_tag="ACFNMK_001941"
     CDS             complement(2467..3000)
                     /locus_tag="ACFNMK_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6365131.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          651 a          834 c          822 g          756 t
ORIGIN      
        1 ctgaaagtcc gagattctgg attcccgctt tcgcgggaat gacggcgaca gggttgctgt
       61 tatagcggat taacaaaaac cagtacggcg ttgcctctcc ttagctcaaa gagaacgatt
      121 ctctaaggtg ctgaagcacc aagtgaatcg gtttcgtact atctgtactg tctgcggctt
      181 cgtcgccttg tcctgatttt tgttaatcca ctataatttc ctgcgtgtgt cgggtgtatc
      241 gaaatcaagc cgaatcaaat atatcggact tcgataatgt cgtattcgcg cacgccgccc
      301 ggggcttgga cttccgccgt atcgccctct tccttgccaa ttaaggcgcg ggcaatcggt
      361 gagccgacat agattttgcc ctgtttgata tccgcttcgt cttcgccgac aatttggtag
      421 gtaacgtgtt cttccgtttc caaatcttcc agcgtaaccg tcgtaccgaa cacgattttg
      481 ccttcggcgt ggatttcggt cggattgatg atgtgggcaa cggaaagttt gtgttccagc
      541 tcggaaatgc ggccttcgat aaagccttgg cgttctttgg cggcttcata ttcggcgttt
      601 tcggacaaat cgccgtgcga tctggcttca gcaatcgctt cgatcacttc ggggcgcgcc
      661 acgcttttaa gctgctgcaa ttcctgtttc agcaattccg caccgcgtac ggtcaggggg
      721 attttttgca tcggtctttt ttctccatat tccggcacac cggtttgcgg cagcaaacat
      781 accgcatacc gtcttgtttt gtgcgtccgg atattaaaat aaaaacacaa gccgcccgga
      841 aaatcggcgg cttgtctgtc gttgaacagc ggctattcta ccaaattcta tgaaattggc
      901 aatcgtgccg tgccgtccgc aaacacgcta tgtccgcaac aaaagccgaa aatatgccgg
      961 caaaaaaatt ttagaaacaa aaaatttaaa aataatcaat tttcggcaca aaaaaccata
     1021 ttcacggact ttaaaaccga aaatgccaaa cctgagattt ttcagacggc atttgcacca
     1081 gtataatgca ggctgttttt atctttatat ttacgttttg ccatgaccga atccgtccgc
     1141 ctccctgccg cctcgctcaa actttccacc gtcgccctgc ccggctccaa aagcatcagc
     1201 aaccgcaccc tgctgcttgc cgccttgtcc gacaatgctt gcgaaatcca ttccctgctc
     1261 aaatccgacg ataccgaccg tatgctcgaa gcactcgata aactcggcgt tcaaatcgaa
     1321 tatcttgccg aagaccgtct gaaagtgcac ggcacaggcg gacgcttccc caaccgcact
     1381 gccgatttgt ttttgggcaa cgcgggcacg gcgttccgcc cgttaaccgc cgctctggcc
     1441 gttttgggcg gcgattatca tctgcacggc gtgcctcgta tgcacgaacg tcctatcggc
     1501 gatttggtcg atgcgttgcg gattgccggc accgatgtcg aatatctcgg caacgaacac
     1561 tatccgccgc ttcatatcgg cgaacgccaa gacaacggcg aacacgtgat tccgattaaa
     1621 ggcaatgtgt ccagccagtt tctgaccgcc cttttaatgg cgttgccact gaccggacag
     1681 gcatttgaaa tccgtatggt cggcgagtta atttccaagc cctatatcga catcaccttg
     1741 aaactgatgg cgcaattcgg cgtacaggtt gccaatgaag actaccgcgt cttcaaaatt
     1801 cccgccgatg cgcactacca cgcgcccgaa cacttgcacg tcgaaggcga tgcctccagc
     1861 gcgtcctact tcctcgcagc cggtttgatt gccgccacgc ccgtccgcgt taccggtatc
     1921 ggcgcaaaca gcatacaggg cgatgtcgcc tttgcccgtg agctggaaaa aatcggggcg
     1981 gacgtggttt ggggcgaaaa cttcgtcgaa gtctcgcgcc cgaaagaacg tgccgtccaa
     2041 gcctttgatt tggatgcgaa ccatatcccc gatgccgcga tgaccctcgc catcgtcgcg
     2101 cttgctacag ggcaaacctg cacgcttcgc aacatcggtt cgtggcgcgt caaagagacc
     2161 gaccgcattg ccgcaatggc gaacgagttg cgcaaactcg gtgcaaaagt cgccgaagaa
     2221 gccgaagcaa ttcacatcac cccgcccgaa acgccgacac ccgacgccgt catcgacact
     2281 tacgacgacc accgcatggc aatgtgtttc tcgctggttt cgctgttggg cgtacccgtc
     2341 gtcatcaacg atccgaaatg cacccacaaa accttcccga cttatttcga cgtgttctca
     2401 tcgctgaccg aaacagcgga ataaggcggc attttgccgc gattccggcg cggcgcggcg
     2461 ggcggctcat tctgtaaaga aagtatgtgc gccgaggtag tttttggcgt aaaacggtgt
     2521 ggagagtttt tcggttttga tggttttgcc gctgctgggg gcatggatga attcgccgtt
     2581 gccgatatag agtccgacgt gtgagtagcg gtgtgcgccg ccggtgttga agaatacgag
     2641 gtcgccggcc ttaaggcggc tgtcggggat tttgcggctt gccgccgcca tgtcgcgggc
     2701 ggtgcgcggc agcttgacgt tgagggcgtt tttgtaaacg aattgaatca tgccgctgca
     2761 atcgaagccg gttgcggtgc tgctgccgcc ccatttgtag ggcgtgccga tgagtccgag
     2821 gctgtggagc atgagttcct gcgagccttg tgtgcggtcg atgtggctga tgcggacggc
     2881 ttggatttgc cggactgtct gtttgggttt cggttggcgg tgtttgccgg aggtcgtgcc
     2941 gcatgaggcg aggagcagtg cgctgagaca gaggaaaagg gttttgtcgg ggggaaacat
     3001 ggtttttcct ttgcgggttc ggatatgccg tctgaaggtg tttcagacgg tatagtggat
     3061 taa
//
LOCUS       DAXHTY010000142         3042 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXHTY010000142 DAXHTY010000000
VERSION     DAXHTY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.142"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            44..2302
                     /locus_tag="ACFNMK_001942"
     CDS             44..2302
                     /locus_tag="ACFNMK_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFC6365132.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFDVCKFLSVAQKELALIRWEAVSIDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDDRSYIATQTVTNPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEAEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLDGSHAAALEK"
     gene            2315..2980
                     /gene="pgmB"
                     /locus_tag="ACFNMK_001943"
     CDS             2315..2980
                     /gene="pgmB"
                     /locus_tag="ACFNMK_001943"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFC6365133.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRHKNDNYVEMIQAVKPEDVYPGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDAIADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSGTAELTYAYLQNVWAQ
                     SGR"
BASE COUNT          774 a          932 c          780 g          556 t
ORIGIN      
        1 atttcaccat tccctcaaat caatccaaac aggagcttca taaatgtaca caagaattat
       61 ggaaatcagc ccttggacgc tgcgttcggc aaaactggaa aaagaacaca aacggctgca
      121 agagagcctg accagtttgg gcaacggcta tatgggtatg cgcggcagct ttgaggaaac
      181 ctactccgcc gacagccact tgggcaccta catcgccggc gtgtggttcc ccgacaaaac
      241 ccgcgtcggc tggtggaaaa acggctatcc caaatatttc ggcaaagcca tcaacgcgct
      301 caatttcagc aaagtcaaaa tctttgtcga tggtcaagag gtggacttgg cgaaaaacga
      361 tgttgccggc ttctccgtcg aactcgatat gcagcacggc gtgttgcgcc gctcgttcac
      421 cgtattcggc gtgcgtttcg atgtgtgcaa attcctgtcc gtcgcacaaa aagagctggc
      481 tctcatccgc tgggaagccg tatctatcga cggcaaaacc caccaagtcc gcatcgattc
      541 catcatcgat gccgacgtga aaaacgaaga ttccaactac gaagaaaaat tttggcaagt
      601 attggacaaa ggcgtttcag acgaccgctc ctacattgcc acccaaaccg tcaccaatcc
      661 cttcggcgtg gaacaattta tcgtcaacgc cgagcaaact ttcgccggca gcttcaaagc
      721 cctcggcggc agccaaaccg actggcaggt ctccaattct ttcgaggccg aagtcggcgg
      781 cacgcccaaa acctttgaaa aacgggttat tgttaccacc agccgcgatt atcagggctt
      841 ggaagcagtg aaagccgcag gccgcgcctt gtcggaaaaa gtcgcaggcg ttgcgtttga
      901 aaccttgctg gacgcgcaca aagcaggctg gctgcaccgt tgggaaatcg ccgacgtggt
      961 catcgaaggc agcgacgaag cacagcaggg catccgcttc aacctgttcc aactgttctc
     1021 cacctactac ggcgaagacg cgcgactgaa catcggcccg aaaggcttta ccggcgaaaa
     1081 atacggcggc gcgacctatt gggacaccga agcctacgcc gtgccgcttt acctcgcact
     1141 ggcggaaccc gaagttaccc gcaacctgct gcaataccgc cgcaaccaac tgccgcaggc
     1201 acaacacaac gcgcgcgaac agggcttggc gggcgcactc tatccgatgg taacgtttac
     1261 gggcatcgag tgccacaacg aatgggaaat caccttcgag gaaatccacc gcaacggcgc
     1321 gattccttac gccatctata actacaccaa ctacaccggc gacgagggct atcttgctaa
     1381 agaaggcttg gaagtcttgg tcgaagtgtc ccgcttctgg gcggaccgcg tccacttctc
     1441 caaacgcaac ggcaaataca tgattcacgg cgtaaccggt ccgaacgaat acgaaaacaa
     1501 catcaacaac aactggtaca ccaacaccct cgccgcatgg gtattggact acacccgcga
     1561 agccttggcg aaatacccgc gtccggattt gaacgtgcgt gccgccgagt tggaaaaatg
     1621 ggcggacatc agcgcgaata tgtaccgtcc gcatgacgaa gaactcggcg tattcgtgca
     1681 gcacgacggc ttcctcgata aagacatccg cccagtgtcc gcgctttcgc ccgacgattt
     1741 gccgctcaac cagaaatggt cgtgggacaa aatcctgcgt tcgcccttca tcaaacaggc
     1801 ggacgtcttg caaggcatct acttcttcgg cgaccgtttc aatatcgacg aaaaacgccg
     1861 caacttcgac ttctacgaac cgatgaccgt gcatgaaagc tcgttgtcgc cgtgtatcca
     1921 ctctattctc gccgccgaac tgggcaaaga agaaaaagcc gtggaaatgt accggcgcac
     1981 cgcccgcctg gacttggaca actacaacaa cgacaccgaa gacggcctgc acatcacctc
     2041 catgactggc tcgtggctcg ccatcgtcca aggtttcgcc caaatgaaaa cctggggcgg
     2101 caaactcagc tttgcaccgt tcctgccggg cgcatggaca ggctacgcct tccatatcaa
     2161 ctaccgaggc cgtctgatta aagtcgccgt cggcaaagaa aacgtcgtct tcaccctgct
     2221 caaaggcgag ccgctcgatt tgcaggtgta tggcaaagac atcacgctcg acggcagcca
     2281 cgccgccgcg ttggaaaaat aaggagggtg caaaatgacg tttaccgcag tcctcttcga
     2341 tctcgacggt gtcatcaccg acaccgccga ataccactac cgcgcgtgga aaaaactcgc
     2401 cgaagaactg ggcatcggca tcgaccgcaa gtttaacgag cagctcaaag gcgtgtcgcg
     2461 cgacgattcg ctcaaacgca tcctcgcgca cggcggtaaa accgtcggcg aagccgagtt
     2521 tgccgaactg acccgccaca aaaacgacaa ctacgtcgag atgattcagg cagtcaaacc
     2581 cgaagacgtg taccccggca ttttgccgct gctggaagca ttgaaagcaa acggcaaaaa
     2641 aatcgccctc gcatccgcca gtaaaaacgg cccgttcctg ctcgaacgca tggggctgac
     2701 ccacttcttc gacgccattg ccgaccctgc cgccgtcgcg cattccaaac ccgcccccga
     2761 catcttcctc gccgccgccg agggcgtggg cgcagacatc cgccgctgca tcggcattga
     2821 agacgctgcc gcaggtgtcg ccgccatcaa agccgccggc gccttaccca tcggcgtggg
     2881 caaagccgaa gacttgggca gcgacatcgc gctggtgtcc ggcaccgccg agctgaccta
     2941 tgcctatttg caaaacgtgt gggcacagtc gggcaggtaa aacgcgtcag ataaagtgtt
     3001 aaggaagtaa aaggccgtct gaacagtgtt tcagacggcc tg
//
LOCUS       DAXHTY010000143         2975 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXHTY010000143 DAXHTY010000000
VERSION     DAXHTY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.143"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            810..1322
                     /locus_tag="ACFNMK_001944"
     CDS             810..1322
                     /locus_tag="ACFNMK_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC6365134.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRQT
                     VQTLLKRLSR"
     gene            1402..2481
                     /gene="aroB"
                     /locus_tag="ACFNMK_001945"
     CDS             1402..2481
                     /gene="aroB"
                     /locus_tag="ACFNMK_001945"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC6365135.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          735 a          872 c          708 g          660 t
ORIGIN      
        1 gtccgttcag tttcggtcgt ttccgataaa ttcctgctgc ttttcatttc tagattccca
       61 ctttcgtggg aatgacggcg gagaggtttt tgttgtttcg gagaagtttc tgcaacccta
      121 gaatctcgtt atttccacaa aaaacagcaa cccaaaacgg caacttaaaa ccccgtcatt
      181 cccgcgcagg cgggaatcta ggtctgtcgg ttcaggacct tatcggataa aacggtttct
      241 ccaaccctgc gttctagatt cccactttcg tgggaatgac gggatatggg tttccgtgcg
      301 gacgtgttcg gatttccgcc tgcgcgggaa tgacggcgac agatgcccaa cggtctttat
      361 agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa atagtacgga
      421 accgattcac ttggtgtttc agcaccttag agaatcgttc tctttgagct aaggcgaggc
      481 aacgctgtac tggtttttgt taatccacta tagtattgat aaacatatta tcttcaatat
      541 attcaattgg ataattgttt acctaagcaa agataattgc cttttcctga caaataagtg
      601 aaatcaacgg attgtcaaaa cacagcctga aataaaaaac ctccctgatt tcttttattt
      661 ttccttaaaa tcagaaaggt tcgggatggt cgggttattt ttccaaacgt accgctgccc
      721 tgccgatttc gtataaaatt ccgccgtaac ccgacaagcc cgaaccctgt cgccccgaaa
      781 ggcggggtgt caaacattaa ggaattgtga tgaaaaactt taacggcaaa ctcatcctca
      841 tcggactgat gggcgcgggc aaaaccacgc tgggccggca aatggcgcag cggctggatt
      901 accgttttta cgacagcgat cacgaaatcg ccgtagccgc cggcgttccc atccccacca
      961 tatttgaaat ggaaggcgaa cagggattcc gttcgcgcga aaccgccata ctcaaaaagc
     1021 tggttatcct gccccatatc gtcctgtcca ccggcggcgg cgcggtgtta aaagaagaaa
     1081 accgcgccct tatccgcaaa agcggcacgg tcgtctatct gcacgccccg cccgaaaccc
     1141 tgctcgaacg cacgcgctgc gacaacagcc gtcctttgct gcaagttgcc gatcctttgg
     1201 cgaaattacg tgaactctac gccgcacgcg accccgttta ccgccaaacc gccgacttta
     1261 ccgtagaatc ggcaaactgc cggcaaaccg tgcaaaccct gctcaaacgc ttatcccgat
     1321 aaaccggcat atgcgccgcg cccagaaaac caagccgcgc cccgcccggc gggccggcgg
     1381 ttcaaacttt aaggaacaac aatgaaaaca ctgaccgtac acaccccgtc ccacagctac
     1441 cccatcttta tcggcaacgg actgctgccg caggcaggaa gcctgctcaa accgcatttg
     1501 ggcaaacgcg ccgccatcat cgccaacgaa accgtcgccc cgctctacct cggcacgctt
     1561 cagacggcat tggatgcggc aggcgtatcc catttcagca tcatcctgcc cgacggcgag
     1621 gcgcacaaaa actggcagac gctcaacctc atctttgacg ggctgatgca aaaccgcgcc
     1681 gaacgcaaaa ccacattaat cgcactgggc ggcggcgtga tcggcgacat ggtcggcttt
     1741 gccgctgcca cctaccagcg cggcgcaccg ttcgttcaaa taccgaccac actgttgagt
     1801 caagtcgact catcggtggg cggcaaaacc gccatcaacc acccgctcgg caaaaatatg
     1861 atcggcgcat tctaccagcc gcaggcggtg cttgccgact tggacacgct gcacaccctg
     1921 cccgtccgcg aattgtccgc cggtatggcg gaagtcatca aatacggcgc gctcggcgac
     1981 atcggttttt tcgaatggct cgaacaaaac atatccggac tgatgacgct cgatcgggaa
     2041 aaactcgccc aagccgtgta ccgctgctgc caaatgaagg cagacatcgt cgcccaagac
     2101 gaaaccgaac agggcatacg cgcatggctc aacctcggac acaccttcgg acacgccatt
     2161 gaaaccgaga tgggttacgg cacttggctg catggagaag ccatcgccgc cggctgcgtg
     2221 ttggcggcgc gtttgtccga acaactgggc aaaacctccg ccgcagatac cgcgcggctc
     2281 gccgccctgc tcgaagccgc tggactgccg tccgcaccgc ccgtgtttgc ctttgaaaaa
     2341 tggctcgaac atatgagcca cgataaaaaa gtcagcggcg gcatcatgcg ctttatcggt
     2401 ctgaaccggc tgggcgaagc caacatcacc gaaattaccg acacggacat cctccgccgc
     2461 accctgcaac cgtatctctg atttcctctg ccgatgtgct gccgcgcggg tttgacgcac
     2521 gatgatgatt ttccatcatc tttctccgta aaagcgggaa tccggtccgt tcggtttcgg
     2581 tcgtttccga taaattcctg ctgcttttca tttctagatt cccactttcg tgggaatgac
     2641 ggtggagagg tttttgttgt ttcggagaag tttctgcaac cctagaatct cgttatttcc
     2701 acaaaaaaca gaaacccgaa acagcaactt aaaacctcgt cattcccgca aaagcgggaa
     2761 tccagtccgt tcggtttcgg tcgtttccga taaattcccg ttgcttttca tttctagatt
     2821 cccactttcg tgggaatgac ggcggagagg tttttgttgt ttcggagaag tttctgcaac
     2881 cctagaatct cgttatttcc acaaaaaaca gaaacccaaa acagcaactt aaaacctcgt
     2941 cattcccgca aaagcgggaa tccggtccat tcagt
//
LOCUS       DAXHTY010000144         2940 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXHTY010000144 DAXHTY010000000
VERSION     DAXHTY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.144"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(127..1437)
                     /locus_tag="ACFNMK_001946"
     CDS             complement(127..1437)
                     /locus_tag="ACFNMK_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC6365136.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDGLKKLVAKVLALPEYRDFTIEADAQELFVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEALKAK"
     gene            1749..2170
                     /locus_tag="ACFNMK_001947"
                     /pseudo
     CDS             1749..2170
                     /locus_tag="ACFNMK_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2858..2940
                     /locus_tag="ACFNMK_001948"
                     /pseudo
     CDS             2858..2940
                     /locus_tag="ACFNMK_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          811 a          721 c          707 g          701 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat accgttctgc ttcagacggc
      121 attgcttcat tttgccttta atgcctcttc atccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagcc
      241 gtcgggcata tagctttcgc cggcggcgta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcatcag cttggtcggg gcgttgcgca ggtggacggg
      361 cacttcgtcg ctagcgtttt ctttgacgaa gtggcgcatt tggttgtatg ccttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaagata caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt ggaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcggtta agtttttcag
      781 acgacgtgta tcggcggcgc gtaaaagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattgacga acagctcctg cgcgtcggct tcaatcgtga aatcccggta
      901 ttcgggcaaa gccaaaactt tggcaaccag ctttttgagg ccgtctgaag acaagggctg
      961 taaaacatac acctgcgcgc ggctcaacag cgcgggattg acttcaaacg acggattttc
     1021 cgtcgtcgca ccaataaagg ttagcaaacc gctttcgaca tgcggcaaaa acgcgtcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgcgc gtccctgctg
     1141 caaagcgatt tcggctttat cgattgcctc gcggatgtcc ttcacgccgg aaaacacggc
     1201 ggaaacaggc aaaaactggg cattgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccgcctt ctaccgccac
     1321 gcgcaaaggt ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcaccggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gattcaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aaaagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 gtgaaaactt tagaaaatta gaacttatta ccactggtaa aaatgataat ggaaatttaa
     1921 cggtaaatgg aatttacggc atccaatctt ttgcagaccc gcaattagga tatacgcctg
     1981 aattatcggt gaaaagcaat aatgatattc tatttaaaaa taatctccaa tctattgacg
     2041 tgaagcccca ttccggaaat atccatttgg atgccaaagg aaaaattcaa tttaatttga
     2101 agttcccgat gttgatgggg tacaagatgt agccagtgga aaaaaatacc gacaaacaat
     2161 agtgccttag tgcggtatgt caacactatc agccccccga tgaaaatgac aaaggcagca
     2221 tatcctttca ttcaaaaaca ggcaatttgt tcaatatgaa cgttaacgtt cataaagaca
     2281 cacaccattt cggtattcac aacctcaaca ataccgatgt tcatctgact gccgaagaca
     2341 gcgataatgt gattgagatt aaaggggaac tcgccgaccc tatgccgaca ggaaatgttg
     2401 agagtacaag ttttttaagg ggcattgaaa cggcttttaa atccaatacc gtattaacgg
     2461 ggaataacaa taaaatatcc atcgccactg agggcaaggg cttgggattg ggcgtaaatg
     2521 gtctccagtc caactccccc tattccggac acacgacggg cattttcatc aataatcaat
     2581 caagcgacct tacccaacct tcccttgatg gcatcagcac catcaaactc aaagccgtca
     2641 agggaaataa tgaagttgat ttaaatgtaa aaaaccacgc ctccgtaaaa ggcattatcg
     2701 cgtcgcattc tgcaaaagca acgcttgaag ccgaagggga caatattgtc caggttaaaa
     2761 atccggacac caatacggct ttaatcgatg ctttgaaaaa acaatacccc aatgcttcga
     2821 aagaactata cagggtgggg gttcaatcca cgactggaac agtacgcatt tcgccaaacg
     2881 gcaaaatcgc attaacggaa ttgaaaactt ttggaattgt acaaaacggc atttgcgtca
//
LOCUS       DAXHTY010000145         2901 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXHTY010000145 DAXHTY010000000
VERSION     DAXHTY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.145"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            35..1252
                     /locus_tag="ACFNMK_001949"
     CDS             35..1252
                     /locus_tag="ACFNMK_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6365137.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGTRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTSIAIIGLANIAGCIF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            1249..1380
                     /locus_tag="ACFNMK_001950"
     CDS             1249..1380
                     /locus_tag="ACFNMK_001950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365138.1"
                     /translation="MKECRLKTLRRHFPNTPPVSGLHDTQISLIKPQTTAAIKPPFK"
     gene            complement(1455..1595)
                     /locus_tag="ACFNMK_001951"
     CDS             complement(1455..1595)
                     /locus_tag="ACFNMK_001951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365139.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            1608..2731
                     /locus_tag="ACFNMK_001952"
                     /pseudo
     CDS             1608..2731
                     /locus_tag="ACFNMK_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          682 a          864 c          683 g          672 t
ORIGIN      
        1 aatccgcaaa cttaaccgac tcaaaataca cattatgacg cacaccgcat cgaaaacacc
       61 caaattttgg ctgaccatca ccgccgccgc attcatcctg ctgattacca tcggcacgag
      121 gatgacgctc ggactgttcg tccaacccgt cgtcaacacc accgaattga gcattgccca
      181 attcagcctc atcatcgccg ttttccaact gatgtggggc atatcgcaac cattgtccgg
      241 cgcgcttgcc gaccgtttcg gcgcgttcag ggtattaagc ggcggcgcag tcctgctcgt
      301 ctgcgcctgc ctgattgccc ccaacatccc gacttattgg gggctgatga ttgccgtcgg
      361 gctgctgctc gcattcggca caggctcagg cggtttctcc atcattatgg ggcaggtcgc
      421 cgcccaagtc cccgcacaca aacgcggctt ggcatccgga ctggtcaacg ccggcggttc
      481 ggcaggacaa ttcctgttcg caccgctggt tcaaggactc gtcgtcctgc ccgaagtcgg
      541 ctggacgggc acattttacg tttggggcgc aatcgccctg ctgattctgc ccgtctcatg
      601 gtggcttgcc ggcggcaaca acgccgccca cacccaacac gcccaagcca cacacggaca
      661 aagcctcgga gaagcagtca aaaccgcctt caaaacccca agctacatcc tgctgcacct
      721 aagctttttt gcctgcggct tccacatcgc ctttctcgta acccacctac ccacggaagt
      781 cgccctgtgc ggactgcccg ccaccgtcgc ctcgacatcc atcgccatca tcggactggc
      841 aaacatcgcc ggctgcattt tttccggctg gtgcacaagc cgcgttcggg gcaaatacgt
      901 cctgttcgga ctgtacgcct cgcgcgccgc gatgatactc atctacattt tttcgcccaa
      961 gaccgacctc aacttctaca ttttcgccgc cgcactcgga tttacctggc tcgccaccgt
     1021 cacgccgacc gcctccatta ccggcaaact cttcggcaca cgctacctcg ccaccctgtt
     1081 cggactgacg atgctcagcc accaaatcgg cggattcctc ggctcgtaca tcggcggcat
     1141 cgtgattaca caattcggcg actacggctg gatgtggtac gccgacgcac tgctggcggg
     1201 aaccgccgcc ctcctcaacc tccccatccg cgaaccccga accgccgcat gaaagaatgc
     1261 cgtctgaaaa ccctcagacg gcattttcca aacaccccgc ccgtttccgg actgcacgac
     1321 acgcaaatca gccttatcaa accgcaaaca accgccgcga ttaaaccgcc attcaaataa
     1381 gtccgttttc ccttcgggtg taaaatcatc acaaaacgcg atactgtata tcgccgggcg
     1441 ttatgcagcg gatttcagga taaaatgccg tctgaagccg cccctgcccg ggcgggcgaa
     1501 gcgtcggatg cggtgcgctt ggcaaaggtt tgcccgccgc gccccgtatc cggcatttca
     1561 tcaatgcaga ccgcatttcc aatcctataa ttcaaggtgt ccgccttatg aaaatcgttt
     1621 ttatcacaac agtcgcatcc agcatttacg gtttccgcgc cccgtcatta aaaaattaat
     1681 cggcaaaaac catcaggtgt atgcctttgt atcggagttt tccgataatg aattggatat
     1741 tatcagggaa atgggggtta cacccgttac ctaccgttca aaccgcagcg ggctgaaccc
     1801 gttttccgat ataaaatcca ccttcctcat ctttaaagaa ctcaaaaaaa tatcgccgga
     1861 tttggttttc ccttatttcg caaaacccgt gattttcggc acttttgccg caaaactggc
     1921 aggcgtgccc agaatcgtcg ggatgctgga aggtttggga ttcgcattta ccccgcagcc
     1981 ggaaggcata ccgttaaaaa caaaaatcat aaaggggatt ttgattgcct tgtaccacat
     2041 tgccctgccg atgttggaaa gcctgattgt attaaacccc gacgacaaag acgaactgac
     2101 ggacaaatac ggcatcaaaa taaaaaacat ccatattttg ggcggaatcg gtctggattt
     2161 gcggcaatat ccttattccg aggcggatat tcccgatgaa aaagaacccg taaaattcct
     2221 ctttatcggc agatttctga aagaaaaggg gattgatgat tttattcggg cggcggaaca
     2281 ggttaaggac aaataccccg atacggtttt taccgctttg ggcgcaatcg acaaatcacg
     2341 cggggggggc gatttggaac ggcttgccgc ccgcgatatt atccgtttcc ccggttttgt
     2401 gaacaatgtt tccgaagtga taaaggcgca tcatatattc gtattgccgt cttataggga
     2461 aggcgttccc cgaagcactc aggaggcaat ggccgtcggc agggcagtga ttacgacgga
     2521 tgtccccgga tgcagggaaa cggtcgccga caaggtcaac ggcttcctga tcgaaccttg
     2581 gaatccccgc atcttggccg aaaaaatgat ttattttatc gaaaacaggg aagccgtccg
     2641 cctgatgggg aatgcaagtt atgcgattgc caaagataaa ttcgatgccg aaaaagtcga
     2701 tttgaaattg ctcgatattt tgaaggcgta aacaaggctg cccgcttttg agtttcggtc
     2761 atttctgata aacctccgtc attcccgcca cacccccgtc attccctcga aagcgggaat
     2821 tcaggttcgt ttagtttcgg tcatttccga taaattcttg caactttgcg tttctagatt
     2881 cccactttcg tgggaatgac g
//
LOCUS       DAXHTY010000146         2714 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXHTY010000146 DAXHTY010000000
VERSION     DAXHTY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.146"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            853..2628
                     /gene="nhhA"
                     /locus_tag="ACFNMK_001953"
     CDS             853..2628
                     /gene="nhhA"
                     /locus_tag="ACFNMK_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin NhhA"
                     /protein_id="HFC6365140.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVETAVLATLLF
                     ATVQASANNEEQEEDLYLDPVQRTVAVLIVNSDKEGTGEKEKVEENSDWAVYFNEKGV
                     LTAREITLKAGDNLKIKQNGTNFTYSLKKDLTDLTSVGTEKLSFSANGNKVNITSDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKKRAASVKDV
                     LNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKKTEVKIGA
                     KTSVIKEKDGKLVTGKDKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSV
                     SLGAGADAPTLSVDGDALNVGSKKDNKPVRITNVAPGVKEGDVTNVAQLKGVAQNLNN
                     RIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGGN
                     WIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          842 a          664 c          651 g          557 t
ORIGIN      
        1 caacgctgta ctggtttttg ttaatccact atatccgttt gcgcccgcca agccgtgttg
       61 cacagatgcg acacggcttt tttctttctg ttgtccaaat gtaaattttc gtcaaaaaac
      121 atcaaaaatc accccccccg aatttgcgta aaataaagtg taatcccttt gattataagg
      181 gaatgttgac aattagatcg attctcctag gctgactttc gcaataaatg gggttgtcaa
      241 taattgtgta tggtcgtttg ccgtccggcg gccgtcattc ccgcgcaggc gggaatccag
      301 caacacaacg cagcaggact ttatcggaaa aaaccgaaac cccaccgacc gtcattcccg
      361 caaaagcggg aatccagcaa ccgaaaaacc acaggaatct atcagcaaaa acagaaaccc
      421 caccgccgtc attcccgcga aagcgggaat ccagacccgt cggcacggaa acttaccgga
      481 taaaacagtt tccttagatt ccacgtccta gattcccgcc ttcgcgggaa tgacgagatt
      541 ttagattatg ggaatttatc aggaatgatt gaatccatag aaaaaccaca ggaatctatc
      601 agaaaaaaca gaaaccccac cgccgtcatt cccgcaaaag cgggaatcca gacccgtcgg
      661 cacggaaact taccgaataa aacagtttcc ttagattcca cgtcccagat tcccgccttc
      721 gcgggaatga cgagatttta agttggggga atttatcaga aaacccccaa cccccaaaaa
      781 ccgggcggat gccgcaccat ccgcccccaa accccgattt aaccattcaa acaaaccaaa
      841 agaaaaaaca aaatgaacaa aatataccgc atcatttgga atagtgccct caatgcctgg
      901 gtcgtcgtat ccgagctcac acgcaaccac accaaacgcg cctccgcaac cgtggagacc
      961 gccgtattgg cgacactgtt gtttgcaacg gttcaggcaa gtgctaacaa tgaagagcaa
     1021 gaagaagatt tatatttaga ccccgtacaa cgcactgttg ccgtgttgat agtcaattcc
     1081 gataaagaag gcacgggaga aaaagaaaaa gtagaagaaa attcagattg ggcagtatat
     1141 ttcaacgaga aaggagtact aacagccaga gaaatcaccc tcaaagccgg cgacaacctg
     1201 aaaatcaaac aaaacggcac aaacttcacc tactcgctga aaaaagacct cacagatctg
     1261 accagtgttg gaactgaaaa attatcgttt agcgcaaacg gcaataaagt caacatcaca
     1321 agcgacacca aaggcttgaa ttttgcgaaa gaaacggctg ggacgaacgg cgacaccacg
     1381 gttcatctga acggtattgg ttcgactttg accgatacgc tgctgaatac cggagcgacc
     1441 acaaacgtaa ccaacgacaa cgttaccgat gacgagaaaa aacgtgcggc aagcgttaaa
     1501 gacgtattaa acgctggctg gaacattaaa ggcgttaaac ccggtacaac agcttccgat
     1561 aacgttgatt tcgtccgcac ttacgacaca gtcgagttct tgagcgcaga tacgaaaaca
     1621 acgactgtta atgtggaaag caaagacaac ggcaagaaaa ccgaagttaa aatcggtgcg
     1681 aagacttctg ttattaaaga aaaagacggt aagttggtta ctggtaaaga caaaggcgag
     1741 aatggttctt ctacagacga aggcgaaggc ttagtgactg caaaagaagt gattgatgca
     1801 gtaaacaagg ctggttggag aatgaaaaca acaaccgcta atggtcaaac aggtcaagct
     1861 gacaagtttg aaaccgttac atcaggcaca aatgtaacct ttgctagtgg taaaggtaca
     1921 actgcgactg taagtaaaga tgatcaaggc aacatcactg ttatgtatga tgtaaatgtc
     1981 ggcgatgccc taaacgtcaa tcagctgcaa aacagcggtt ggaatttgga ttccaaagcg
     2041 gttgcaggtt cttcgggcaa agtcatcagc ggcaatgttt cgccgagcaa gggaaagatg
     2101 gatgaaaccg tcaacattaa tgccggcaac aacatcgaga ttacccgcaa cggtaaaaat
     2161 atcgacatcg ccacttcgat gaccccgcag ttttccagcg tttcgctcgg cgcgggggcg
     2221 gatgcgccca ctttgagcgt ggatggggac gcattgaatg tcggcagcaa gaaggacaac
     2281 aaacccgtcc gcattaccaa tgtcgccccg ggcgttaaag agggggatgt tacaaacgtc
     2341 gcacaactta aaggcgtggc gcaaaacttg aacaaccgca tcgacaatgt ggacggcaac
     2401 gcgcgtgcgg gcatcgccca agcgattgca accgcaggtc tggttcaggc gtatttgccc
     2461 ggcaagagta tgatggcgat cggcggcggc acttatcgcg gcgaagccgg ttacgccatc
     2521 ggctactcca gtatttccga cggcggaaat tggattatca aaggcacggc ttccggcaat
     2581 tcgcgcggcc atttcggtgc ttccgcatct gtcggttatc agtggtaagg gctttttcgc
     2641 ctgtctgctg ttgggacagg cggaaggttt gaagggaagg gtggcgattt gccgcctgag
     2701 acctttgcaa aatc
//
LOCUS       DAXHTY010000147         2654 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXHTY010000147 DAXHTY010000000
VERSION     DAXHTY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.147"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            424..2592
                     /gene="fetA"
                     /locus_tag="ACFNMK_001954"
                     /gene_synonym="frpB"
     CDS             424..2592
                     /gene="fetA"
                     /locus_tag="ACFNMK_001954"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC6365141.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAGNANVALDTITVKGDRQGSKI
                     RTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNAY
                     SDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLDK
                     NWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKTV
                     PYSALDKRSYLAKIGTTFGDDDHRIVLSHMKDQHRGIRTVREEFTVGSEDSRINIKRQ
                     APAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNHT
                     RITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSKFEISDKKKDPKDPKKEI
                     DKTAEEKAKDKKDMDLVHSYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKT
                     HDGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADG
                     TKAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKN
                     HGYELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRF
                     QNPNLEIGWRGRYVQKAVGSILVAGQKDRNGKLENVVRKGFGVNDVFANWKPLGKDTL
                     NVNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          742 a          767 c          600 g          545 t
ORIGIN      
        1 ttcgttcggt ttcagtcatt tccgatagat tcctgccgcg ttaggggttc tagattcccg
       61 ctttcgcggg aaggacgcgg aaaggttgct tttcgtcatt cccgcatttg ttaatccact
      121 atatttccgc cgttttttat agtggactaa caaaaaccag tacagcgttg cctcgccttg
      181 ccgtactatc tgtactgtct gcggcttcgt cgccttatcc tgatttttgt tagtccacta
      241 tacatttccg acaaaacctg tcaacaaaaa aacaacactt cgcaaataaa aacgataatc
      301 agctttgcaa aaatcccccc ccctgttaat ataaataaaa ataattatta attatttttc
      361 ttatcctgcc aaatcttaac ggtttggatt tacttccctt catacactca aaaggacgaa
      421 caaatgaacg ccccgttttt ccgcctcagc ctgctctcgc tcacacttgc ggcaggcttt
      481 gcccacgcgg caggcaacgc caatgtcgca ttggatacca ttaccgtaaa aggcgaccgc
      541 caaggcagca aaatccgtac caacatcgtt acgctgcaac aaaaagacga aagcaccgca
      601 accgatatgc gcgaactctt aaaagaagag ccttccatcg atttcggcgg cggcaacggc
      661 acgtcccaat tcctgacgct gcgcggcatg ggtcagaact ctgtcgacat caaggtggac
      721 aacgcctatt ccgacagcca aatcctttac caccaaggca gatttattgt cgatcccgct
      781 ttggttaaag tcgtttccgt acaaaaaggc gcgggttccg cctctgccgg tatcggcgca
      841 accaacggcg cgattatcgc caaaaccgtc gatgcccaag acctgctcaa aggcttggat
      901 aaaaactggg gcgtgcgcct caacagcggc tttgccagca acgaaggcgt aagctacggc
      961 gccagcgtat tcggaaaaga gggcaacttc gacggcttgt tctcttacaa ccgcaacgat
     1021 gaaaaagatt acgaagccgg caaaggtttc cgcaatgtca acggcggcaa aaccgtaccg
     1081 tacagcgcgc tggacaaacg cagctacctc gccaaaatcg gaacaacttt cggcgacgac
     1141 gaccaccgca tcgtgttgag ccatatgaaa gaccaacacc ggggcatccg cactgtgcgt
     1201 gaagaattta ccgtcggcag cgaagattca cggataaata ttaaacgcca agcccctgct
     1261 taccgcgaaa ccacacaatc caacaccaat ttggcgtaca cgggtaaaaa cctgggcttt
     1321 gtcgaaaaac tggatgccaa cgcctatgtg ttggaaaaag aacgctattc cgccgatgac
     1381 agcggcaccg gctacgcagg caatgtaaaa ggccccaacc atacccgaat caccactcgt
     1441 ggtgcgaact tcaacttcga cagccgcctt gccgaacaaa ccctgttgaa atacggtatc
     1501 aactaccgcc atcaggaaat caaaccgcaa gcgtttttga actcgaaatt tgagatctcc
     1561 gataagaaga aagatccgaa agatcccaaa aaagaaatag ataagaccgc tgaagaaaaa
     1621 gcgaaagaca agaaagatat ggatcttgtc cattcctaca aactgtccaa cccgaccaaa
     1681 accgataccg gcgcgtatat cgaagccatt cacgagattg acggctttac cctgaccggc
     1741 gggctgcgtt acgaccgctt caaagtgaaa acccacgacg gcaaaaccgt ttcaagcagc
     1801 aaccttaacc cgagtttcgg tgtgatttgg cagccgcacg aacactggag cttcagcgcg
     1861 agccacaact acgccagccg cagcccgcgc ctgtatgacg cgctgcaaac ccacggcaag
     1921 cgcggcatca tctcgattgc cgacggcacc aaagccgaac gcgcgcgcaa taccgaaatc
     1981 ggcttcaact acaacgacgg cacgtttgcc gccaacggca gctacttctg gcaaaccatc
     2041 aaagacgcgc ttgccaatcc gcaaaaccgc cacgtctctg ccgccgtccg tgaagccgtc
     2101 aatgccggtt acatcaaaaa ccacggttac gaattgggcg cgtcctaccg caccggcggc
     2161 ctgactgcca aagtcggcgt aagccacagc aaaccgcgct tttacgatac gcacaaagac
     2221 aagctgttga gcgcgaatcc tgaatttggc gcacaagtcg gccgcacttg gacggcctcc
     2281 cttgcctacc gcttccaaaa tccgaatctg gaaatcggct ggcgcggccg ttatgttcaa
     2341 aaagccgtgg gttcgatatt ggtggcaggt caaaaagacc gcaacggcaa attggaaaac
     2401 gttgtacgca aaggtttcgg tgtgaacgat gtcttcgcca actggaaacc gctgggcaaa
     2461 gacacgctca atgttaatct ttcggttaac aacgtgttca acacgttcta ctatccgcac
     2521 agccaacgct ggaccaatac cctgccgggc gtgggccgcg atgtacgctt gggcgtgaac
     2581 tacaagttct aaaacgcaca tcccgaaaaa atgccgtctg aaagcctttc agacggcatc
     2641 tgttctgata attt
//
LOCUS       DAXHTY010000148         2587 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXHTY010000148 DAXHTY010000000
VERSION     DAXHTY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.148"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            98..655
                     /locus_tag="ACFNMK_001955"
     CDS             98..655
                     /locus_tag="ACFNMK_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC6365142.1"
                     /translation="MGFGCREGKEFCKGLSLPEIIRDLLEMPSETAVADIARTYSAHY
                     LNPNNRNMTLFPDALPCLDKLKAQGYWLAVATGKGRAGLDNAISQTATGGYWLATACA
                     GEYPSKPSPEMVFGICGELGLDPKEALVVGDTAHDLHMAANAGAAAVGVTTGAHSREQ
                     LRTAPHLAVLDGLSELPGFLAQHYA"
     gene            complement(742..1710)
                     /gene="ispH"
                     /locus_tag="ACFNMK_001956"
                     /gene_synonym="lytB"
     CDS             complement(742..1710)
                     /gene="ispH"
                     /locus_tag="ACFNMK_001956"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC6365143.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAEERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1739..2266)
                     /gene="lspA"
                     /locus_tag="ACFNMK_001957"
     CDS             complement(1739..2266)
                     /gene="lspA"
                     /locus_tag="ACFNMK_001957"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC6365144.1"
                     /translation="MVLFYRLLKNMSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQ
                     YRERVNVIPSFFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEF
                     ATLGKTGAAMIIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLA
                     VLDNIVHRKTQEEKH"
BASE COUNT          588 a          770 c          648 g          581 t
ORIGIN      
        1 gaccggatgc ctgattatcg ggtatccggg gaggattaag ggggtatttg ggtagaatta
       61 ggcggtattt ggggcgaaaa cagccgaaaa cctatgtttg ggtttcggct gtcgggaggg
      121 aaaggaattt tgcaaaggtc tcagcctgcc cgaaatcatc cgcgacctgc tcgaaatgcc
      181 gtctgaaacc gccgttgccg acatcgcacg cacctattcc gcgcattacc tcaatcccaa
      241 caaccgcaat atgacgctct ttcccgatgc cctgccctgt ctggacaagc tcaaagcaca
      301 aggatactgg cttgccgtcg ccacgggcaa agggcgggcg ggcttggaca acgccatcag
      361 ccaaaccgcc accggcggct attggctcgc caccgcctgc gcgggggaat atccctccaa
      421 accctcgccc gaaatggtat tcggaatctg cggcgaactg ggactcgatc cgaaagaagc
      481 attggtcgtc ggcgatacgg cgcacgacct gcatatggcg gcaaacgcgg gggcggcggc
      541 agtcggcgtg accaccggcg cacattcgcg cgaacagctc cgcaccgcac cgcatctcgc
      601 cgtattggac ggtttgtccg aactgcccgg ttttcttgca caacattacg cctgattggc
      661 ttccgcatcc ggcacgcggc aaaaatgccg tctgaagcct gttcagacgg catttgtttt
      721 gcccaaacat tcaacgcttg ctcaacgttt gcacaaatcg ggtttggttt cgccctcgcg
      781 gcgcaactct ttgggcagga cgaacacgat gctttcttcc gcgccctccc cttcgcgcac
      841 ggtttcgtgc ccccatccgc gtatggttgc caatacttcc cgcaccaaca cttcgggcgc
      901 ggacgcgcct gccgttacgc cgactttgtt tttgccctca aaccatgcgc gttgcaggta
      961 gcctgcatta tccaccatat acgcatcgat tccgcgcgat gccgccactt cgcgcaagcg
     1021 gttgctgtta gacgaattgg gcgaaccgac cacaatcacg atgtcgcact gttctgccaa
     1081 ctctttgacg gcggtttgcc ggttggtcgt cgcatagcag atatcttcct tgtgcggatt
     1141 gcggatattg gggaaacgcg cgttcagcgc ggcgatgatg tctttggttt catcgaccga
     1201 gagcgtggtt tggctgacat aggcgagttt gtcggggttt ctgacttcga gttttgccac
     1261 atctccgacc gtttcgacca aaagcatttt gcccggcgca agctgcccca tcgtgccttc
     1321 gacctcgacg tgccccttat gcccgatcat gatgatttca cagtcttggg catccagtcg
     1381 ggcgacttcc ttatgcactt tcgtcaccag cgggcaagtc gcatcaaaca cgcggaaacc
     1441 acgttcctcc gcttcttgct gcaccgcctt cgatacgccg tgtgccgaat aaaccagtgt
     1501 cgcgcccggc ggcacttccg ccaagtcttc gataaacacc gcgccttttt cgcgcaggtt
     1561 gtccacgacg aatttgttgt ggacgacttc gtggcgcaca taaatcggcg cgccgaactc
     1621 ttccaaagca cgttcgacaa tactgattgc ccgatccaca ccggcgcaga agccacgcgg
     1681 attagcaagg atgatggttt tctcgttcat aagcccggta tttcgttttc agacggcatc
     1741 aatgtttttc ttcttgggtt ttacggtgga cgatgttgtc caacactgcc aacaccgcac
     1801 cgacgcagat aaagctgtcg gcaatattaa aggcgggata aaaccaattt tgccaataaa
     1861 acaataagaa atcgacgaca tgaccgtgta tcaggcggtc gatgacattg cctaacgcac
     1921 cgccgataat catcgccgca cccgttttgc cgagggttgc aaactcatcg cgcaagatgg
     1981 cgcgtaccaa atacgcgctc accgccaccg ccagcaccaa aaaaaagtat ttttgccagc
     2041 cgccctgatc ggcaaggaag ctgaacgccg cgcctgggtt gtacaccaag gtcaaatcga
     2101 aaaacgaagg gatgacattg acgcgttccc gatactgaaa cgacgacagc accgcccact
     2161 tcgaccactg gtccagcacg atggcggcaa gtgccaatac ccaatagcgc gttttacttg
     2221 aaacagatga agacatattt ttcaacagcc ggtaaaagag taccatttta cccgaaaacc
     2281 ccctttcctg tacccgaaac ggcaaatgcc gtaatcttaa aacccgtcat tcccgacaac
     2341 accgtaatct cgaaacccgt cattcccgcg taggcgggaa tccagacctg tccgcacaga
     2401 aacttatcgg ataaaaacag ttgcccaaac cccgcgttct atagtggatt aaattcaaac
     2461 cagtacggca ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc
     2521 gagtgaatcg gttccgtact atttgtactg tctgcggctt cgttgccttg tcctgattta
     2581 aatttaa
//
LOCUS       DAXHTY010000149         2574 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXHTY010000149 DAXHTY010000000
VERSION     DAXHTY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.149"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            52..272
                     /locus_tag="ACFNMK_001958"
                     /pseudo
     CDS             52..272
                     /locus_tag="ACFNMK_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(319..1728)
                     /gene="dacB"
                     /locus_tag="ACFNMK_001959"
     CDS             complement(319..1728)
                     /gene="dacB"
                     /locus_tag="ACFNMK_001959"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC6365145.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTINDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERGTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMVVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1893..2459
                     /locus_tag="ACFNMK_001960"
     CDS             1893..2459
                     /locus_tag="ACFNMK_001960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC6365146.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          609 a          613 c          717 g          635 t
ORIGIN      
        1 atgtattaga tgtgcgcgaa tttagccatt ttagcttcgc tgaaacttcg ttttcgtatc
       61 aatcacagca cacattttgc cgaacgacaa aaccatatca atggaattga gaacttttgg
      121 aaccgggcaa aacgtcattt acgcaagttt gacggcattc ccaaagcgca ttttgagctg
      181 tatttaaagg agtgcgaatg gcgttttaac aacagtgaga taaaagttct tgttccattt
      241 taaaacaatt agtaaaatcg agtttatcct agttatctag gacagcccct taaatttaat
      301 ccactatatt ttcctgtttc aggctcggcg ttctttgcac atcagtttcg catccagcca
      361 gccatcgccg ccggagatga tgttgttggc aacgaagttg tccaagtcgg gcagcaggga
      421 aacggcgcga ccgctgttga tgatgacgac caccaccatc ggtttgtcgc ccagccagta
      481 gcctgccaat gcgcggacat tgttgagcgt gccggttttc aggcgcagaa gcccaccgct
      541 ttgtttgaag cggttgcgta aagttccgtc tgtgccggcg atgggcagcg tgtcgatgaa
      601 atcttgtgca aacgggctga aataagccgt ttccaacatt tgcgccatca ttctcgccgt
      661 tcccctttct tttctagaca gacccgaacc gttttccaaa accaaatccg caacatcgat
      721 gcccgacacg gcaagttcgc gccggacggc agacgctgcc tgttcggaaa cggcgggcag
      781 tttgccgtcg ccgccgagtt tgaggaagac ggaacgcgca attagattgt ccgaacgctt
      841 gttcatgtcc gtcaaaattt ccttcatcgg tttggcgtgt gcaacggcaa gcgtctgcgc
      901 gccttccggc gtgtcggcta tgccgatacc gtctgaaatc cgtccgccgc cgagcagcca
      961 gtggttggta aaactttgcc ggatcagttc gtcaagcgcg aacatccgga caccgacagg
     1021 cttgcccaaa cagctctcgg gaatattgcc gcgcaatttc agcgtattgt ccgaaaaaga
     1081 tgcacgcatc agttttttga tcgaagggca ggcagcttgg gaggcggtaa ttttcaagtt
     1141 gttttgggcg aaaatatgcg gcaaaggcgg atcggtgagg atgtcggtac tgtcggcggc
     1201 attgcgttcg gcgcgcacca taaccatacc ggcagacagc atagttggat tggggggcgt
     1261 cataaacggc gaaccgctgt cggcttcgaa atcgtcgggg ctgccgactt cgccccacag
     1321 gctgtggtcg agcatcaggt gtcccgtgat attgcgtatg ccttgttcgc gcaactggcg
     1381 ttggacggca agcaggtttt cctgattgaa aacggggtcg ccgctgcccg cccaatacag
     1441 gtttccgtca agcgtgccgt cgtttatcgt accgttgctt ttaaactcgg tcgcccagcg
     1501 gtaattgctg ccgaaggttt tgaaggcggc aaacgcggta acgagtttca ttgtggaggc
     1561 ggggttgacg gggacatccg agcggtggtc aatgatgact tttccgctgt caagctcttg
     1621 gacatatacg gcgatttcgt tttgcggaat gcggccggta tcgagcgcgt gtgcggcgag
     1681 ggaggcgaga agcagcagca gggaggccgc tgttttgggg aaattcatag gtaaatctgt
     1741 tacataaaaa aagcaattat aaggcaaagc cggataagtg ggaacgctgt ggcggcgggc
     1801 cggcttgttt gcaggggaaa tcacatatat aataatcgct atcattatga aatgattgag
     1861 acgcacaaac ttaatattca ggaggaatga ttatggctaa gaaaatcagt attttggtgg
     1921 gcagtctgcg ccgtgcttct tttgcgcgca aagtggcatt gaatgcggcg gagatgttcc
     1981 ccgaaggctg gcaggcggaa atcgtcgaaa tcggacatct gccgctttac aatttcgatt
     2041 atgacgaccc tgcggtggaa gatgtgccgc tgcccgaaag ctacacggtt ttccgcgaaa
     2101 cgattaaggc ttcggacggc attttgttcg ttacgtccga aaataaccgc accattcccg
     2161 cctgtttgaa aaatgcggtg gacatcggct cgaaaccgaa tgccgacgtg gcgtggaaaa
     2221 acaaaccggc cggcatcatc agccattccg tcggcaagat gggcggttac agttcgcaaa
     2281 aaaacctgcg ccttgcgctg tcttatttcg atatgcccgt aaccggacag ccggaagtgt
     2341 ttttgggcaa ttcgccgaca ctgtttgatg aaaacggcaa attgatcgac tcggcaaggg
     2401 attttgttca gtcatatatt aatcagtttg tcggtttgat tgaaagaaat gccaaataaa
     2461 caaacaagtc gaaaggccgc aaaaccggtt cgggttttgc ggctttttta tatgggctgg
     2521 gcagcgtcaa ggtttgccgt ttgccggata atgcgcggca aatgaccggc gtgt
//
LOCUS       DAXHTY010000150         2532 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXHTY010000150 DAXHTY010000000
VERSION     DAXHTY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.150"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            281..1408
                     /gene="fic"
                     /locus_tag="ACFNMK_001961"
     CDS             281..1408
                     /gene="fic"
                     /locus_tag="ACFNMK_001961"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6365147.1"
                     /translation="MSNWKPNIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLENWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
     gene            1438..2316
                     /locus_tag="ACFNMK_001962"
     CDS             1438..2316
                     /locus_tag="ACFNMK_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6365148.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLLVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSTILGVVLEVKKRF
                     MSAAQARRRVDSDYHP"
     gene            2320..2532
                     /locus_tag="ACFNMK_001963"
                     /pseudo
     CDS             2320..2532
                     /locus_tag="ACFNMK_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
BASE COUNT          710 a          638 c          620 g          564 t
ORIGIN      
        1 tagtgtatat taagtacgtc tgatatacac gataccctac gagggtgtaa gctttagttc
       61 acatttaaaa tgacctcttt aaacctgtct ttcggcaggt ttctttttag gttgtttgga
      121 aatcgtgtgc agacaaggtg taaaataggt aacagcataa aataatgcgg ttttaccgcc
      181 catatattta caaaagccaa atttttaaac atatatcctt gatatataca cggcgtaaac
      241 atatactgga aacatcttta aattttccga aattttaaat atgagcaact ggaaacccaa
      301 tattccctat aacgatttac cacccctgcc gccaaaacag gatattgaaa gcaaaaccat
      361 cctgaaacgt tgtatagccg cccgtgcatc ccttgcccgt ttaaagcagg cggcagaact
      421 gataccgaat caagccatgc tgattaacac ccttcctgtt atggaagccc gtgcaagttc
      481 ggaaattgaa aacatcgtaa ccaccacgga caagctgttt caatccctgc aaatggatac
      541 ggaacggcaa gaccctgcca cgaaagaagc cctgcaatac cgcaccgccc tgtttgcagg
      601 ctatgaatca ctgacgagcc gccctttatg cacacaaacc gccatcatgg tttgcaacgc
      661 catcaagcac ccctacgaaa tggccatccg caaaacaggc ggcacagccc taaaaggagg
      721 caacagcgga aatgttgtct ataccccgcc cgaaggagaa gaaaccatac gcggcaagct
      781 ggaaaattgg gagcggttta ttcacgaaag cggcgattta gacccgctta tcatcatggc
      841 ggcggcacat taccaatttg aagccatcca cccgtttacg gacggcaacg ggcggacggg
      901 acgcatattg aacagcctgc tattgattga aaaagggctt ttggatttgc ctattttgta
      961 tttgagccgc tacatcatcg aaaacagggc ggactattac cgcctgctgt taggcgtaac
     1021 cgaacggcag gattgggaaa gctggataat ctacatctta gacggcgtag ccgacaccgc
     1081 cgattggacg gtatcgaaaa tagatgcgat acgccgcctg ttcgagcaga cacggcaaca
     1141 catacggaca cacgcacaag gaatctacac gcacgaactg gtaaatcttc tgtttgagca
     1201 gccatataca cgcattgcca acctggaagc ggcagggata gccaaacggc agacggcctc
     1261 taagtacctg aaagagcttt cagatatagg tgtgctgcaa gaaatcgcca tcggcaggga
     1321 caaactattc attcatccgc gcctaatgga actattgcgg ggagagggca acagctttac
     1381 ctcattccaa tccctcgtta aagcatagcc aaaataatca ataatccgga ggtcaatatg
     1441 gcaagaaggt caaaaacatt tgaagaagct gctgctgagg ttgaggaacg tttcggtcat
     1501 cgtggcatta agttggtcga gtttgagggt acagccaagc cgtgtgtaat caactgccct
     1561 aaacatggaa accaaacctg ttcgaggtac tccaatatgt tcataggaag tagctggggt
     1621 tgcccctctt gtggtaatga gcaagctgca aaagccggta tagcgaccct taggaagaat
     1681 cacatagcgt tagaaatgct gaaacaggct gtaacaggta tgaccaagca agagcgcatc
     1741 acgacgcaag cctacaatga gatgaccaaa tccgtggcag gttcaaacag catagtcctt
     1801 aacgatgtcc aaggcgatac gaccatcaac aaccatcata cgcatacgca caaccacagc
     1861 gatgccgatg gcaaagcact gtcgatgagg ctcacacccc gtcctttgtt gtcagaccgt
     1921 caggcggcgg ctttcgcccg tacaggcaaa ctcacgggca gtttcgacct gtttgcttcg
     1981 gtggtcgccc cctcgcagta cacgtttgcc gttgccatgc ccgacacgtc catgtcgccg
     2041 gttatcgaaa agggagactt gctggtggtc gagccgcgta tgcgccctgc ggacgaggac
     2101 atcgtgctga ttgaactgtc cgacaagcgg ctggtcgtcg cgcaccttgt tatcgatatt
     2161 gcgggcagga tgctgattta tcagacgggc aggccgtctg aagcctttga cctgcccgaa
     2221 ggcagcacga ttttaggtgt ggtgctggag gtcaaaaaac ggtttatgtc cgccgcacag
     2281 gcgagaaggc gtgttgattc ggattaccac ccctgatgtg tggacggttg gtacgatttc
     2341 cgcttccaaa acgtcgtgta cgcgcccgac cgcagcccgg aaatcagccg tatgctttct
     2401 tcgattttgg caggctacgc gtgggatacc gaaaacccgt tcgtggcgaa atccgaacaa
     2461 cgcctgactg ccttgtcaga atgggtcggt cagctggaaa ccgaatatag tgaattaaat
     2521 ttaaaccggt ac
//
LOCUS       DAXHTY010000151         2432 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXHTY010000151 DAXHTY010000000
VERSION     DAXHTY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.151"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            35..1390
                     /locus_tag="ACFNMK_001964"
     CDS             35..1390
                     /locus_tag="ACFNMK_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HFC6365149.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="ACFNMK_001965"
     CDS             1394..2404
                     /locus_tag="ACFNMK_001965"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFC6365150.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDGLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          437 a          667 c          740 g          588 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcagatg agccgcatct tccagacgct
      181 cggtgccgat ccgcacagcc tcggctggtt ctttatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggat
      781 cgaactcttg aaaaccgcgc ctaaggcgtt ttggacggtt actttggtgc aattcttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta ccaagaagcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttctgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat ttcggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acggcctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggccgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaacggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgcat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg ac
//
LOCUS       DAXHTY010000152         2422 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXHTY010000152 DAXHTY010000000
VERSION     DAXHTY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.152"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            500..575
                     /locus_tag="ACFNMK_001966"
     tRNA            500..575
                     /locus_tag="ACFNMK_001966"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:534..536,aa:Glu,seq:ttc)
     gene            960..1125
                     /locus_tag="ACFNMK_001967"
                     /pseudo
     CDS             960..1125
                     /locus_tag="ACFNMK_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
     gene            complement(1175..1981)
                     /gene="thiD"
                     /locus_tag="ACFNMK_001968"
     CDS             complement(1175..1981)
                     /gene="thiD"
                     /locus_tag="ACFNMK_001968"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC6365151.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCKAVRTAKSYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          578 a          616 c          614 g          614 t
ORIGIN      
        1 cgtatgctac cgcatactgg cctttttctg ttagggaaag ttgcacttca aatgcgaatc
       61 cgccggtttc ttgagatttt gcgttctaga ttcccgcttt cgcgggaatg acgaaaagtg
      121 gcgggaatga cgaagtttca gacggcatcg cccgcctgtt ttgatatggc ggcacccccc
      181 ccgacaaaaa aaacaatccg gaacgcatct gaccgttccg gcttgttttc aggcgaatcc
      241 gccgcatcag aacatactgc gcacgcccat attgacctgc caagtctggc gcatcgtgtg
      301 catcgaagac ctttgcgcct caaaataaag ctgccttccg ttgtcggcat taccacgcaa
      361 aaaaatgaat tgcttgatat tccaatgttt tttatatgtt tttatattgt gatgcgatca
      421 gacaaacgcc cccctgacat ttgtttagac ggcatcgtat tgctaaattt ctataagtat
      481 gtataatgtc cgtttccacg cgcccatcgt ctagaggcct aggacactgc cctttcacgg
      541 cggcaaccgg ggttcgaatc cccgtgggcg tgccaattca aaaacctgct tgtttcaagc
      601 aggttttttt attatgaatc gtcattcccg cacccttcgt cattcccgcg aaagcgggaa
      661 tctggagcgc aggattaaag aaaccgtttt atccgataag cttccgtgcc gacaggtctg
      721 gattcccgcc tgcgcgggaa ggacggcaga gggtggacga tgccgtctaa agcctgacaa
      781 ggcatttgat gccgtctgaa acttcgtcat tcccgcaccc ttcgtcattc ccgcaaaagc
      841 gggaatctag agcgtagggt tgaagaaacc gttttatcca ataagtttcc gtgccgacag
      901 gtctggattc ccactttcgt gggaatgacg agtcttttat aacctgagac ctttgcaaaa
      961 tgcctttccc ttttaaattt aaagaggggg gagctgaagg attattatcg ggattgggag
     1021 ctggtcgaat ataaagaaga attgggcgcg atgcacgcca aagacgaaaa cggcaatccg
     1081 atacggttta aatttgtaac catgctggcg aaaaagcctg aataatcctc aaaaatgccg
     1141 tctgaacatt ttgtttcaga cggcattttt acgtctagtc ccgatacgcc caatgattga
     1201 ccgggccgtg tcccgcgccg atttccaaag ggtttgagat tgccgccgta atgtaggact
     1261 tggcagtccg tacggctttg caaacgtccg agccttttgc caactcggcg gtgatgcagg
     1321 cggaaaacgt gcagcccgtg ccgtgcgtgt gggcggttgg aaagcgcggg ctgtccaatt
     1381 ccagcgtttc attttgcgta aacagccaat ccgtgcagcg tccgcttgtg ctgccgttca
     1441 aatgtccgcc tttgataacg acatttttga caccgtaatc aagcaggatt tttgccgcac
     1501 gttccgcatc tttacggttt tcgatatgca cgccggtcag ggcttccgct tcaggcaggt
     1561 tgggggttaa tacatccgta tcgggaagca gtaggcgcgt cagtgccgca acggcggaat
     1621 cctgcaacag cggcgcaccg cctttggcaa tcatcacagg gtcgagtacg cgcctgccaa
     1681 agctgcagtg tttcagcttg tcggcaacac attcgatgat ttccgccgtg ccgagcattc
     1741 ctattttgta ggcgcggatg tcaaagtctt ccctaattgc ttggatttgt gcggtgatgg
     1801 tttcggtcgg gacgagatga accgccgaca cgcccaaggt attttgcgcg gtaacggcgg
     1861 tgatgacgca cgttccgaac acgccgcgca tctgaaacgt tttcaaatcc gcctgaatgc
     1921 ccgcaccgcc gcccgaatcc gaaccggcga tggtaagcgt ttgcacaaaa gagcttttca
     1981 tttgttcctc cttaatattg tgtccgataa aaagggctaa tgcctgtaaa aaagcacggc
     2041 gatgccgtct gaaaaccgca gcgcggggtt tgtggtgcat tatatgccgt attgcaggac
     2101 aaggcacaga gcagggcggc ggggtgcatc gtagcaactg tatttttgcc cgacggggtg
     2161 aaaaatacag ttgctacacc cactgataat gaaaaaagag agcaatgccg tctgaaccgc
     2221 gtgttgcggc gagcataaac cggcacgctt tattcgtcat tcccacaaaa acagaaacct
     2281 aaaattccgt cattcctacg aaagcgggaa tccagtgtgt tcggtttcag ttgtttttgg
     2341 gtttcgggtg atttccaaat cgaaaccttt gcaaaattcc ccaaaatccc ctaaattccc
     2401 accaagacat ttaggggatt tc
//
LOCUS       DAXHTY010000153         2405 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXHTY010000153 DAXHTY010000000
VERSION     DAXHTY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.153"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..497
                     /locus_tag="ACFNMK_001969"
     CDS             <1..497
                     /locus_tag="ACFNMK_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6365152.1"
                     /translation="SVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDA
                     CSLSTTMGERLLTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITH
                     RNYHRYGENDYSTSVGSCINGFTVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNN
                     PSKK"
     gene            514..>2405
                     /locus_tag="ACFNMK_001970"
     CDS             514..>2405
                     /locus_tag="ACFNMK_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365153.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTENGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNAQA
                     VNNPNGSEWDNRKQLISALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIETVATKGFAGSLFDHTNNGIRTATGWVSADDGLLVR
                     DLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVW
                     QDLNQDGISQANELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTDGTTAKM
                     GDLLLAADNLHSRFKDKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSA
                     AETKEAQLALLDNLIHKWAETDSNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKN
                     ALVSLSDKAKAAIDAARDRIAVLDAYTGQDSNTLYYMSEEDALNIVKVTNDTYDHLAK
                     NIYQNL"
BASE COUNT          757 a          482 c          551 g          615 t
ORIGIN      
        1 ttagcgtcta ttacaaaaaa accgaccaag gctgggttct tgagccatac aaccagcaaa
       61 acaaagcaca ctttatccaa tttctacgcg acggtttgga tagcgtggac gatattgtta
      121 tccgaaaaga tgcgtgtagt ttaagcacga ctatgggaga aagattgctt acttacgggg
      181 ttaaaaaaat gccatctgcc tatcctgaat acgaggctta tgaagataaa agacatattc
      241 ctgaaaatcc atattttcat gaattttact atattaaaaa aggagaaaat ccggcgatta
      301 ttactcatcg gaactatcat aggtatggag agaacgatta cagcactagc gtaggttcct
      361 gtattaacgg tttcacggta cggtattacc cgtttattcg ggaaaagcag cagctcacac
      421 agcaggagtt ggtaggttat caccaacaag tagagcaatt ggtacagagt tttgtaaaca
      481 atccaagtaa aaaataattt aaaggatctt attatgaatg agggtgaagt tgttttaaca
      541 ccagaacaaa tccaaacctt gcgtggttat gctttccgtg gcgataccta tggcggttgg
      601 cgttatttgg ctaatttggg tgaccgttat gcggatgatg ctgctgcaat tgtcggtaag
      661 gatgcaaact taaatggttt gaatttatgg atgaaaaaag gtgtggaaaa cctatgggat
      721 gatacggtcg gtaaaaagac ccgtttagag aaatttgatc gggttgcatt gcaacatttc
      781 agccaatatg tagatctaat taatgaaaat aatggtagat tacctaacac tagtgaaatt
      841 gagagaagtt actataaagc cgttaccgaa aatggtgttt cttctagtgc agctattgat
      901 ttagttatta atcgctcact tccggatatg gcagatggtt attgggcatt aggtttgggg
      961 atagaagccg aacgtatcca caatgcgcaa gcagtaaata atccgaacgg tagcgaatgg
     1021 gataatagaa agcagttaat atctgcttta gataaaggat ttgatggatc ttttaaagag
     1081 aagcatttta cttttttaca atctgtgata atggatgtaa caaagttagg tgttgaatat
     1141 acaatagatg gttggcaaaa aattggaggt tggggtaatg ggataatcaa tgatttatat
     1201 aaaagtgttg caaaaagaga gtggactgga atatttgaga tcgttaataa taacatcaag
     1261 caatttagag atctgttccc aaatccggaa ggctggatcg atgatggtca ccaatgtttc
     1321 gctccttggg ttaaagaaac taaaaaacgc aatggcaaat atcatgtcta cgaccccctc
     1381 gccctagatt tggacggaga cggtatagaa accgttgcca ccaaaggctt tgcaggcagc
     1441 ttatttgatc acaccaacaa cggtatccgc accgccaccg gttgggtttc tgccgatgac
     1501 ggtttactcg tccgcgattt gaacggcaac ggcatcatcg acaacggtgc ggaactcttc
     1561 ggcgacaaca ccaaactggc agacggttct tttgccaaac acggctacgc ggctttggcc
     1621 gaattggatt caaacggcga caacatcatc aacgcggcag acgccgcatt ccaaaccctg
     1681 cgtgtatggc aggatctcaa tcaggacggc atttcccaag ctaatgaatt gcgtaccctt
     1741 gaagaattgg gtattcaatc tttggatctc gcctataaag atgtaaataa aaatctcggt
     1801 aacggtaaca ctttggctca gcaaggcagc tataccaaaa cagacggtac aaccgcaaaa
     1861 atgggggatt tacttttagc agccgacaat ctgcacagcc gcttcaaaga caaagtggaa
     1921 ctcactgccg aacaggcaaa agccgccaat cttgcgggca ttggtcgtct gcgcgatttg
     1981 cgcgaagctg ccgcattgtc cggcgatttg gccaatatgc tgaaagctta ttctgccgcc
     2041 gaaactaaag aagcacagtt ggcattgtta gataatttga ttcacaaatg ggcggaaacc
     2101 gattcgaact ggggcaaaaa atcgccaatg cgactttcaa ccgattggac gcaaacggct
     2161 aatgaaggta ttgcactgac accatcccaa gtagcacaac taaaaaagaa cgctttagtt
     2221 tccctttctg ataaagctaa agcagctatt gacgccgccc gcgaccgcat tgccgtgctt
     2281 gatgcctaca cggggcagga ttccaacaca ctctattaca tgagcgagga agatgcgctt
     2341 aatatcgtca aagtaaccaa cgatacatac gaccatctcg ccaaaaacat ctaccaaaac
     2401 ctgtt
//
LOCUS       DAXHTY010000154         2393 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXHTY010000154 DAXHTY010000000
VERSION     DAXHTY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.154"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(105..230)
                     /locus_tag="ACFNMK_001971"
                     /pseudo
     CDS             complement(105..230)
                     /locus_tag="ACFNMK_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            complement(559..969)
                     /locus_tag="ACFNMK_001972"
     CDS             complement(559..969)
                     /locus_tag="ACFNMK_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365154.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIKGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(973..1728)
                     /locus_tag="ACFNMK_001973"
                     /pseudo
     CDS             complement(973..1728)
                     /locus_tag="ACFNMK_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218290.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(1798..2115)
                     /locus_tag="ACFNMK_001974"
     CDS             complement(1798..2115)
                     /locus_tag="ACFNMK_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365155.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(2112..>2393)
                     /locus_tag="ACFNMK_001975"
     CDS             complement(2112..>2393)
                     /locus_tag="ACFNMK_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC6365156.1"
                     /translation="NKPVVKSIRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDGV
                     KIDIRPNGEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
BASE COUNT          675 a          457 c          421 g          840 t
ORIGIN      
        1 gggatttgca gcaacgatat tgctgctgca aacagcatca tgaatttttg aataggcaaa
       61 ttcattttgt tttccttggt agcaagcggt tatggtagtg tgaatcaagg ttgcccttgt
      121 ctatgttgtc gcactgcttc atcgctgtat ccatacccct catgactgtt atcagcctct
      181 acggatgggg cggagttacc cgtatgattg ccgtatggtc ggatacttgt atccgacaaa
      241 aatatttgac gcgtctatcg tttccgaaat gccgctgttg aaaaacgtcg gattcgagaa
      301 tccgacctac ggttgctgaa caatgttttg tagaacttgc tcaaaatcca tgtttcgcca
      361 ttttccttta tatataccta atgcagcacg ggttacgatt tcgtttaagt cataaatagc
      421 catttacgtc ctctgctaat tttcagcgag tagattgggt taccaaaccc aacaaaaaag
      481 cagcctgaaa aatttcagga tgcttgtttt atgtttttta tcagtgaatt aggttttaat
      541 ccagcctgcg cttgctagtt aaacagtatt tacataaaaa tcttgcactg aacaaacaag
      601 cgtatcatgt tcatcatgaa atgatgggca taacgttaat ctacaaatgt ttacatttaa
      661 agatattttt tcagtttcaa tatttttaat ttgtaaggtt aatacatgag gatatttgtg
      721 atatagctga ttttttaatt gttcgttgtc taatatagca aaaggtaatt ttgcacacaa
      781 ataaccaata actttgtttt tatgaaaaaa aactatccaa ttttcaattc cttcatccca
      841 atcaaaagta aaatccgttc cttttatgat aaatttagca caagatatta aatctaactt
      901 aacccaagtg gatttataca gactcaatgc tgatgaagtt ataatattat ctaaattata
      961 aattttcata aatcaacgtt ccttacctgg tgttccatgc acaggtctaa acccatcttt
     1021 attcggcttt ctacgataaa tgttaattat atgtgtcggt tttccgtctg tatagccaac
     1081 tatgtcgttc gttggaactt ttgccttatt ttgactatcc cataaattat gtttatctgc
     1141 atatctagca gcatctaatg tagctttttc cgcatcaaca tctgatttaa ataaattttt
     1201 gctatctatc ttgtctagac tagatgcatc aggtttgtgt cgtaccatac tgagagggtc
     1261 agtaatatat ttaccatctg taatagctga tccaccatta taggtagtat gcgtataatt
     1321 tttttgtgga acgagactat tttgcaaaga ttgttgtgta gaaacgccag ctttgccatt
     1381 aggagtagct tgcttcgctg gcttattccc accggccatt tccccatcta aagtctgcat
     1441 aggtcgggca gcaggtgttt tataatcggt atttttaacc caatcccatt tagtcgggtt
     1501 aagttctact tttttaccgc gccaaaccgt acctgcggcc tctgctacgg caagggcagt
     1561 ttgggctgtt gcagttatat tcggatgggc atcagcccat tgtctggcgg aattgatacc
     1621 gtcttttacc gcaaaagcac tatcctgtaa taggctcgcg gcagcaagtt gtgtttccgg
     1681 acttaaattt cctaaattat taataccttg tagagtctgc tgcgcagaga tgtagcaact
     1741 gtatttttac cccgtcgggt aaaaatacag ttgctacggc tgctgagaaa gcagttacta
     1801 ttttcccagt ggctcaaaat taattgtttc agcagatatt tttataataa tccctacatc
     1861 aataataatt tcactataaa attggtattt atatagctct atttcgggta taaacaaggg
     1921 aaacaagcta tcttccttat cctcataatc tacgaaatta tttgtatcta atttaaaatt
     1981 caaaatatta ctgcatttga tatatccgaa ataattacct tcaattgtat ctgtaaaaga
     2041 taatgtaagg gtatttccat tatcggaaaa gattatttca taaaggctta cattgtgaaa
     2101 aatatttttt ttcatttgac aaaatatcca gaatgatgat gattatttgg caatggattg
     2161 ccttcataat cttgtcgttg cggatatttt ccctgtacac catcggttcg cggcactctt
     2221 tgggttctta taacctctcc atttggacga atatctattt tcacgccatc aggacctttc
     2281 catgtttcat attgagccgc atctgaacca gtacgtctaa aaccttgttc ttgcaatgct
     2341 tgacgtaatt catctcgcgt agttggtctg atagatttaa caactggctt gtt
//
LOCUS       DAXHTY010000155         2261 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXHTY010000155 DAXHTY010000000
VERSION     DAXHTY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.155"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..567)
                     /locus_tag="ACFNMK_001976"
     CDS             complement(<1..567)
                     /locus_tag="ACFNMK_001976"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC6365157.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFSACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAK"
     gene            730..2136
                     /gene="dnaB"
                     /locus_tag="ACFNMK_001977"
     CDS             730..2136
                     /gene="dnaB"
                     /locus_tag="ACFNMK_001977"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC6365158.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          546 a          583 c          608 g          524 t
ORIGIN      
        1 tttggcgact tcgtcccagt tgacgatttc ccagaaacct ttcaggtagt tgggacggct
       61 gttgcggtag tcgatgtaat aggcgtgttc ccacacgtcg caggtcagca gcggcgtgtt
      121 ttcagtggtc agcggagttg ctgcgttgga agtggaaacc aaatccaatc cgccggcagg
      181 ggttttcacc agccacgccc aaccggagcc gaacgtaccg gcagcgcaag cggagaatgc
      241 ttcttggaat ttctcgaagc tgccccattt cgcgtcgatg gcggcggcca gttcaccgga
      301 aggtttgcct tgacctttgg gcgtgaaacc cagccagtag aaagtgtggt tccaagtttg
      361 ggctgcgttg ttgaacacgc cgcccgaaga ttttttcaca atctcttcca aaggcaggtt
      421 ttcaaattcg gtgcctttga tttgattgtt caggttggtg atgtaggttt gatggtgttt
      481 gccgtagtgg aattccaaag tctctttgct cagatgcggg gacaatgcgt ccagttcata
      541 aggcagttgc ggcagcttat gttccatttt gtgctcctaa tattgtttta aatgttgtat
      601 tttggcagtg ttgctgcaaa tagcttggca gcgcacatat tttacctgtt ttgaggaatg
      661 gaaaccaatt aaacctgctt tacgctataa tagaagattg caatttcggc acgacagata
      721 ggatgtacca tgaacgatta cgcagccatg ccgtctgaag accgtgaggt cggcgcattg
      781 tcgcttccgc cgcactcaat ggaggcggaa caatccgttt tgggcgggtt gatgctggaa
      841 aatccggcat gggacaggat tgccgatgtg gtttcgggag aggatttcta ccggcatgaa
      901 caccgcctga ttttccgttc cattgccaaa ttaatcaatg agagccgtcc cgcagatgtg
      961 attacggttc aggaagattt gcagcggaac gaagaattgg aagcggcagg cggattcgaa
     1021 tacctgatta cgctggcgca aaacaccccg tctgccgcca acatccgccg ctacgccgaa
     1081 atcgtgcgcg agcgttccat catgcgccaa ctcgccgaag tggggacgga aatcgcccgc
     1141 agcgcataca atccgcaagg cagggacgcg gggcagcttt tggacgaggc ggaaaacaaa
     1201 gtattccaaa tcgccgaaag cacggccaaa tccaagcagg gctttttgga gatgcccgat
     1261 ttgctgaaag aagtcgtaca gcgcatcgat atgctctact cgcgcgacaa tcccgatgaa
     1321 gttaccggcg tgccgacggg gttcatcgac ctcgacaaaa aaacctcggg tctgcaaccc
     1381 ggcgacctga ttatcgttgc cggtcgtccg tctatgggta agaccgcctt ttctatcaat
     1441 atcgccgaac acgttgccgt agaaggcaag ctgcccgttg ccgtcttttc gatggagatg
     1501 ggcggtgcgc aactggtcat gcgtatgctc ggctcggtcg gacggttgga tcaaagcgtc
     1561 ctgaaaacag gcaggctcga agacgaacac tggggtcgtc tgaatgaagc agtcgtcaaa
     1621 ctctcagacg cgcccgtgta catcgacgag accccgggtc tgactgcgct cgaactgcgc
     1681 gcccgtgccc gccgtctcgc ccgtcaattt aacaataagc tgggattaat cgtcatcgac
     1741 tacctgcaac tgatggcggg atccggccgt tccgacaacc gtgcttcgga gcttggcgaa
     1801 atttcccgct ccctcaaagc cctggcaaaa gagttgcaag tccccatcat cgccctgtcg
     1861 caactgagcc gcacggtcga atcgcgtacc gacaaacgcc cgatgatgtc cgaccttcgc
     1921 gagtccggcg caatcgagca ggatgccgac ctgattatgt tcatgtatcg cgacgaatac
     1981 tacaaccaag actcacccat gaaaggcctt gccgaatgta tcatcggcaa acaccgcaac
     2041 ggtcccgtcg gtaaaatctt cctcacatgg acgggacaat tcaccaaatt cgacaatgct
     2101 gcctatattc ccgaggaggc aaagatagag gattaaatgg ctatataaaa atttattagg
     2161 cgaaatcagg caaaatcgtt taaaatcatg ctgagagatt gccctaaaaa ataaaacgcg
     2221 gtcttgaggc atttttgcat tcagcccgca tataattgaa a
//
LOCUS       DAXHTY010000156         2249 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXHTY010000156 DAXHTY010000000
VERSION     DAXHTY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.156"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..>2249
                     /gene="pilC"
                     /locus_tag="ACFNMK_001978"
     CDS             <1..>2249
                     /gene="pilC"
                     /locus_tag="ACFNMK_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6365159.1"
                     /translation="TKPYAIVMNGQKLPEVKWGQSYNSLLQKSNPRQVNFTTSFSGYK
                     KNTTFSFNNTDEVVAQKNGVTVFGAATYLPPYGKVSGFDEKRLKERGDVLGWIGTTHP
                     GLVGYSYEGNTCSSGDCPDVSYKTQFTFGNQGLKSKVNGKLDIYEDKSRDNSPIYKLP
                     DQPWLGVSFNLSSESVVESKKLKKVESSFNEDVTQNNGAQSPYKDTNLVYTTGDGNNK
                     GNQVHQDKHHAVVFYLNAKLHLLDKKQIKNITQVRELNLGELKTRIEPTNAWKNKRHL
                     TFNVGNWEFKDTGLVSVKLKLPQVKAGRCTNAAHPNLKAKLPSPALTAPALWFGPVQN
                     GKAEMYSASVSTYPDSSSSRIFLQNLKRKTDPGRPGRYSLADLNASDIQSKEPTFTGR
                     QTVIRLDDGVQQIKLDKSNEATGLNGNTNNNTFGIVKEYSVNPETNEWKKVLLPWTVR
                     GFSNDNEFVKFNKESDKYSQRYRSRDNSKRERDLGDIVNSPIVAVGEYLATSANDGMV
                     HIFKKGNGDARNYSLKLSYIPGTMPRKDIESKDSTLAKELRAFAEKGYVGDRYGVDGG
                     FVLREVERGGKKHVFMFGAMGFGGRGAYALDLTKADDNDPTKASLFDVKDNGNNGNNG
                     NNRVELGYTVGTPQIGKTHNDKYAAFLASGYATKEITSGENKTALYVYDLENNNGTPI
                     AKIEVKDGKGGLSSPTLVDKDLDGTVDIAYAGDRGGNMYRFDLSGQDPSQWTVRPIFE
                     GTKPITSAPA"
BASE COUNT          663 a          554 c          564 g          468 t
ORIGIN      
        1 accaagccat acgctattgt gatgaacgga caaaagctgc ccgaggtaaa gtgggggcaa
       61 tcatataatt cattgctgca aaaaagcaac ccccgccaag ttaactttac gacatctttt
      121 agtggataca aaaaaaacac tactttctca ttcaataata ccgatgaagt tgttgctcaa
      181 aaaaacggtg ttaccgtttt cggcgcggcg acctacctgc cgccctacgg caaggtttcc
      241 ggttttgatg aaaagaggct gaaagagcgc ggcgatgtcc ttggttggat tggtacgacc
      301 cacccggggt tggtaggcta cagctacgaa ggtaacacat gctccagcgg agactgtcct
      361 gatgttagct ataaaaccca atttaccttc ggtaatcaag ggttgaaaag caaggtaaac
      421 ggcaagctgg atatatacga agacaaaagc cgcgacaatt cgcccattta taaattgccg
      481 gatcagcctt ggttgggcgt gtctttcaat ttaagcagcg agagcgtcgt cgaatcaaag
      541 aaactcaaga aagtggaatc ttcttttaac gaggatgtga cgcagaacaa tggtgcgcaa
      601 agcccataca aagacacaaa cctcgtttat acgacaggcg atggtaataa taagggtaat
      661 caagtccatc aggacaaaca ccacgccgtc gtcttttatc tgaacgccaa actgcacctg
      721 ctggataaaa aacaaatcaa aaatatcacg caagtcaggg aacttaattt gggtgagttg
      781 aaaacgcgca tcgagccgac gaacgcgtgg aagaataaga ggcatctcac tttcaatgtt
      841 ggtaattggg agttcaaaga taccggatta gtcagcgtca agctcaaatt gccgcaagtc
      901 aaagccgggc gctgcaccaa cgccgcccac cccaatctca aggccaaact cccttcgccg
      961 gcactgactg cccccgcgct gtggttcgga cctgtgcaaa atggtaaggc ggagatgtat
     1021 tccgcttcgg tttccaccta ccccgacagt tcgagcagcc gcatcttcct tcaaaatctg
     1081 aaaagaaaaa ccgaccccgg cagacccggc cgctattctc tcgcagactt gaatgcgtcg
     1141 gatattcaaa gtaaagagcc gactttcaca gggcggcaaa ctgtcatccg attggatgac
     1201 ggcgtacagc agatcaaact ggataaaagc aatgaggcca ccggtttaaa tggaaatacc
     1261 aacaacaaca ctttcggcat tgttaaggaa tatagcgtca atcctgagac caatgagtgg
     1321 aaaaaagtgc tgctgccttg gacggttcgg ggttttagta atgacaatga atttgtaaaa
     1381 ttcaacaaag aatcagacaa atacagccaa agataccgca gccgcgataa cagcaagcgc
     1441 gagcgtgatt tgggcgacat cgtcaacagc cccatcgtgg cggtcggcga gtatttggct
     1501 acttccgcca acgacgggat ggtgcatatc ttcaaaaaag gcaacgggga cgcgcgcaac
     1561 tatagtctga agctcagcta catccctggc acgatgccgc gcaaggatat tgaaagcaaa
     1621 gactccaccc ttgccaaaga gctgcgcgcc tttgccgaaa aaggctatgt gggcgaccgc
     1681 tacggcgtgg acggcggctt tgtcttgcgc gaagtcgaac ggggcgggaa aaaacacgtg
     1741 tttatgttcg gcgcgatggg ctttggcggc agaggcgcat acgccttgga tttgaccaaa
     1801 gccgacgaca atgacccgac aaaagcctct ttgtttgatg taaaagataa cggcaataat
     1861 ggcaataacg gcaataatcg cgtggaatta ggctacaccg tcggcacgcc gcaaatcggc
     1921 aaaacccaca acgacaaata cgccgccttc ctcgcctccg gttatgcgac taaagaaatt
     1981 accagcggcg agaataaaac cgcgctgtat gtgtatgatt tggaaaacaa caacggtacg
     2041 ccgattgcaa aaatcgaagt gaaggacggc aagggcgggc tttcgtcccc cacgctggtg
     2101 gataaagatt tggacggcac ggtcgatatc gcctatgccg gcgatcgcgg cgggaatatg
     2161 taccgctttg atttaagcgg tcaagatcct agtcaatgga ctgtacgccc tatttttgaa
     2221 ggcacaaaac cgattacttc cgcgcctgc
//
LOCUS       DAXHTY010000157         2133 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXHTY010000157 DAXHTY010000000
VERSION     DAXHTY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.157"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            60..182
                     /locus_tag="ACFNMK_001979"
                     /pseudo
     CDS             60..182
                     /locus_tag="ACFNMK_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            433..1890
                     /locus_tag="ACFNMK_001980"
     CDS             433..1890
                     /locus_tag="ACFNMK_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HFC6365160.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPIVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          496 a          612 c          490 g          535 t
ORIGIN      
        1 cgtaccggtt tttgttaatc cgctataatg cgcccttcgg cgtggcggat atataaggaa
       61 gtgattttcc atctaagtat agtggattaa atttaaatca ggacaaggcg acgaagccgc
      121 agacagtaca aatagtacgg caaggcgagg caacgccgta ctggtttaaa tttaatccac
      181 tataaaaaac cgccctatcg gataagccct taacagaaaa ggctttaccc gcgccgtatc
      241 ggaacacatc ctctaaaata caatccgttg aattgaaaaa aatataaaaa catccgcccg
      301 cgaaaaacgg cagcgcgtcg tttgacaaag aatgaaaata tcggttaaaa accgattttc
      361 atacaaaaaa caccgctgcc gtccgcatcc gtttcagacg gtattgagag aaaatctttt
      421 aggagaacct ttatgtcccg gcatcccgcc cccaccggag aaaaaacatt cttcggccac
      481 cccttccagc tttccaccct cttccatatc gaattgtggg aacgtttttc attttacgga
      541 atgcagggca tcctgctgat ttacctctac tacaccgccg acaaaggcgg cttgggcata
      601 gacaaaaccc tcgccggcgg cattgtcggc gcatacagcg gcagcgtgta cctgtccacc
      661 attttggggg cgtggtttgc cgaccgagta tggggtgcgg aaaaaaccct cttcctctcg
      721 ggcatcgtcg tgatgctcgg acacatcgtc cttgccgccg ccccgggcct gtacggcctt
      781 ttaatcgggc tgatattcat cgcattgggc agcggcggcg tgaaatctac ggccagttct
      841 atggtgggcg cattatacga acaggacgaa atgcgcccgc tgcgcgatgc gggattttcc
      901 attttctaca tcgccatcaa tatcggcggc ttcctaggcc cgctgctgac cggcctgctg
      961 caggaaaaca tcggtttcca ttatggtttc ggcgcggcgg cggtcggtat ggcattcggc
     1021 ttgtggcgtt attccttggg acgtaaaaac ctgccccatc ccatcgtccc ccatccgctt
     1081 tcaaaaggac agggcaaaac tgcggccgcc gtcggcattg ctctcatcgc cgcacttgca
     1141 accgccatca aaaccgggct tgtcaacctc gacaatttct ccggcatcct cttatctacc
     1201 gtcatccttg ccgtcattgc ctattttgcc cgcctgctga ctaatccccg cgtcagttcc
     1261 gacaacaaac ggcacatcat cgcctacatc ccgcttttcc tgaccatctg tatgttttgg
     1321 gcggtctggt ttcagattta caccgtggca accgtctatt tcgacgaaac cgtcaaccgc
     1381 accatcggtt cgtttaccgt acccgtcgct tggaaagatt ctatgcaaag cctgtgggtc
     1441 atcctgtttt ccggactgat ggcggcaatg tggacaaaaa tggggcgcaa acagcccaaa
     1501 accccgctga aattcgctat ggcggtattt gttaccggcg cgtcgttttt gggattcgtc
     1561 ccctttattt cctccggtac gccgatgcct attgcggttt tcgcactgat tgtccttgcc
     1621 atcacgatag gcgaactgat gatttccccg attgcgctgt ccatctccac caaaatcgca
     1681 ccgcctttat tcaaaaccca aatggtcgcc cttaatttcc ttgccttttc attaggcttc
     1741 actttgggcg gcgtattgtt tgaaaaaggc tatcaggcgg gcgacgaaat cggcttctat
     1801 cggctgctgt tctacatcgg cgcagccaca ggcttcctgc tgctcctgct cgtccccaaa
     1861 ttgaacaaaa tgctcgaagg cacagactaa gtcccgcccc gatgccgtct gaacccttca
     1921 gacggcattt ttccgcataa tgaaaccaaa ccgtttccac ccgacaggac aggctccccc
     1981 caaccggaag gcagcctgcc gattgtcatt taaatatctg actgattccg tctgtttgca
     2041 agggaaatag ccgtttattt ccgcttgggc ggaaaccggt tctattgagt aaaaggcatt
     2101 ttatccgact aaattagtgc tgcatcaacg gaa
//
LOCUS       DAXHTY010000158         2072 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXHTY010000158 DAXHTY010000000
VERSION     DAXHTY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.158"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..197)
                     /locus_tag="ACFNMK_001981"
     CDS             complement(<1..197)
                     /locus_tag="ACFNMK_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365161.1"
                     /translation="MAERCRPGAKMRHGAAGGAHACRGKENRERRWAETLPEEKAGLR
                     GGKRGMRRAESACVGSKGGYN"
     gene            390..1808
                     /locus_tag="ACFNMK_001982"
     CDS             390..1808
                     /locus_tag="ACFNMK_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6365162.1"
                     /translation="MNIHPLLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHSKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT          450 a          661 c          477 g          484 t
ORIGIN      
        1 aaattataac cccccttact gccgacgcac gcactttccg cgcggcgcat tccccttttc
       61 ccgcccctca aacccgcctt ttcttcaggc agggtttcag cccacctcct ttccctgttt
      121 tcctttcccc gacacgcatg cgcgccccct gccgcaccgt gccgcatttt cgcgcccgga
      181 cggcatcgtt ccgccatccg gttctctgtt ttaaataccc ctgtttcaga aagaaatgca
      241 gatgtttcaa cacacaggac gacacataaa gcaccgccct atgtgttgcc ctgatttgga
      301 aggggttacg cctcccaaat aaagtctgat cctgccgccc cgaaggacag atgtccgagt
      361 ggcgaagttt caaccgaaaa ggaaatacga tgaatattca ccccctgctc tccaaacaat
      421 ggacgctgcc gccattcctg ccgaaacggc tgctgctgtc cctgctgata ctgcttgccc
      481 ccaatgcggt gttttgggtt ttggcactgc tgaccgccac cgcccgcccg attgtcaatt
      541 tggactatct tcccgccgcg ctgctgatcg ccctgccttg gcgtttcgtc aaaattgccg
      601 gcgtattggc gttttggctg gcggttttgt ttgacgggct gatgatggtg atccaactct
      661 tcccttttat ggatctcatc ggcgccatca acctcgtccc cttcatcctg accgcccccg
      721 ccccttatca gataatgacc gggctgttgc tgctgtatat gctggcgatg ccgtttgtgt
      781 tgcagaaagc cgccgccaaa accgacttcc ggcacattgc cgtctgcgcc gccgttgtgg
      841 cggcagccgg ctatttcacc ggccatttga gttactacga ccggggtcgg atggccaata
      901 tcttcggcgc aaacaacttc tactacgcca aaagtcaggc gatgctctac accgtcagcc
      961 agaatgccga ctttattacc gccggcctgg tcgatcccgt cttcctcccc ttgggcaatc
     1021 aacagcgtgc cgccacgcat ctgaacgagc cgaaatctca aaaaatcctc tttatcgtcg
     1081 ccgaatcttg ggggctgccg gccaatcccg aacttcaaaa cgccactttt gccaaactgc
     1141 tggcgcaaaa agaccgtttt tcggtttggg aaagcggcag ttttcccttt atcggcgcga
     1201 cggtcgaagg cgaaatgcgc gaactgtgcg cctacggcgg tttgcgcggg ttcgcactgc
     1261 gccgcgcgcc cgacgaaaaa tttgcccgct gcctccccaa ccgtttgaaa caagaaggtt
     1321 acgccacctt tgcgatgcac ggcgcgggca gttcgcttta tgaccgcttc agctggtatc
     1381 cgagggcggg ctttcaagaa atcaaaaccg ccgaaaacct gatcggtaaa aaaacctgcg
     1441 ccattttcgg cggcgtgtgc gacagcgagc tgttcggcga agtgtcggca tttttcaaaa
     1501 aacacagcaa aggattgttt tactggatga cgctgaccag ccacgccgac tatcccgaat
     1561 ccgacatttt caaccacagg ctcaaatgca ccgaatacgg cctgcccgcc gaaaccgacc
     1621 tctgccgcaa cttcagcctg cacacccaat tcttcgacca actggcggat ttgatccaac
     1681 gccccgaaat gaaaggcaca gaagtcatca tcgtcggcga ccatccgccg cccgtcggca
     1741 acctcaatga aaccttccgc tacctcaaac aggggcacgt cgcctggctg cacttcaaaa
     1801 tcaaataaca acaatgccgt ctgaacgcac caacagcctt cagacggcat tttgcagaca
     1861 gaccgaccct tcaagcccac ttttttcatc atctccgata aattgctttg tatagtggat
     1921 taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggta
     1981 ctgaagcacc aagtgaatcg gttccgtact atttgtactg tctgcggctt cgtcgccttg
     2041 tcctgatttt tgttaatccg ccataaagac cg
//
LOCUS       DAXHTY010000159         1978 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXHTY010000159 DAXHTY010000000
VERSION     DAXHTY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.159"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            30..821
                     /locus_tag="ACFNMK_001983"
     CDS             30..821
                     /locus_tag="ACFNMK_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6365163.1"
                     /translation="MNPAPKNLLFSSLLFSSLLFSSLLFSSAAQAASEDSGRGPYYVQ
                     ADLAYAAERITHDYPKPTGTDKDKISTVSDYFRNIRAHSIHPRVSVGYDFGDWRIAAD
                     YASYRKWKESNSSTKKVTEEINNNYKETQTKHQGNGSFHASSSLGLSAIYDFKLNDKF
                     KPYIGARVAYGHVKHQVHSVESKTTIVTPKPSGGPVIEGPTPKPPYHESNSISSLGLG
                     VIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
BASE COUNT          529 a          600 c          432 g          417 t
ORIGIN      
        1 atcagttaaa accgaatacg gagccgaaaa tgaatccagc cccaaaaaac cttctcttct
       61 cttctcttct cttctcttct cttctcttct cttctcttct cttctcttcc gcagcgcagg
      121 cggcaagtga agacagcggg cgcggcccgt attatgtgca ggcggattta gcctacgccg
      181 ccgaacgcat tacccacgat tatccgaaac caaccggtac agacaaagac aaaataagca
      241 cggtaagcga ttatttcaga aacatccgtg cgcattccat ccaccctcgt gtgtcggtcg
      301 gctacgactt cggcgactgg aggatagcgg cagattatgc cagttacaga aaatggaaag
      361 aaagcaattc ttctactaaa aaggttactg aagagataaa caacaactac aaagaaaccc
      421 aaacaaaaca tcagggaaac ggcagcttcc acgcctcttc ttctctcggc ttatccgcca
      481 tttacgattt caaactcaac gataaattca aaccctatat cggtgcgcgc gtcgcctacg
      541 gacacgttaa acatcaggtt cattcggtgg aaagcaaaac cacgattgtt acccctaaac
      601 caagtggagg ccctgtcata gaaggtccga cccccaaacc tccctatcac gaaagcaaca
      661 gcatcagcag cttgggtctt ggtgtcatcg ccggtgtcgg tttcgacatc acgcccaagc
      721 tgaccttgga caccggatac cgctaccaca actggggacg cttggaaaac acccgattca
      781 aaacccacga agtctcattg ggcatgcgct accacttctg attccccgat accgatgccg
      841 tctgaacctt cagacggcat ttttgatgca ctcgccgttt acaggcgcgg ggcgggcgtg
      901 gaaatacccg aaccgtcatt cccgacaaca ccgtaatctt gaaacccgtc attcccgcac
      961 aggcgggaat ccagacctcc gacgcggcgg gaatctatcg gaaatgactg aaacccccga
     1021 gattctagat tcccacttcc gtgggaatga cgattcagtt tcgttccgac aacaccgtaa
     1081 tctcgaaacc cgtcattccc gcacaggcgg gaatccagac ctccgacgcg gcgggaatct
     1141 atcggaaatg actgaaaccc cgagattcta gattcccact tccgtgggaa tgacggttca
     1201 gttgcgctcc gacaacaccg caatcttgaa acccgtccga caacaccgta atcttaaaac
     1261 ccgtcattcc cgcgcaggtg ggaatccaga ccccctgacg cggcgggaat ctatcggaaa
     1321 tgactgaaac cccgagattc tagattccca cttccgtggg aatgacgatt cagtttcgtt
     1381 ccgacaacac cgtaatctcg aaacccgtca ttcccgataa cagcgcaatc ttgaaacccg
     1441 tcattcccga caacaccgta atctcgaaat ccgtcattcc cgcacaggcg ggaatccaga
     1501 cctccgacgc ggcgggaatc tatcggaaat gactgaaacc ccgagattct agattcccac
     1561 ttccgtggga atgacgtggt gcaggtttcc gtatggatgg attcgtcatt cccgacaata
     1621 ccgcaatctc gaaattcgtc attcccgcgc aggcgggaat ccagccccct gacgcggcgg
     1681 gaatctatcg gaaatgactg aaacccccga gattctagat tcccacttcc gtgggaatga
     1741 cggttcagtt gcgttccgac aacaccgtaa tctcgaaacc cgtccgacaa caccgtaatc
     1801 ttgaaattcg tcattcccgc acaggcggga atccagaccc ccgacgcggc gggaatctat
     1861 cggaaatgac tgaaaccccg agattctaga ttcccacttt cgtgggaatg acggttcagt
     1921 tgcgttccga caacaccgta atctcgaaac ccgtccgaca acaccgtaat cttgaaac
//
LOCUS       DAXHTY010000160         1792 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXHTY010000160 DAXHTY010000000
VERSION     DAXHTY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.160"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(433..1422)
                     /gene="porB"
                     /locus_tag="ACFNMK_001984"
     CDS             complement(433..1422)
                     /gene="porB"
                     /locus_tag="ACFNMK_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HFC6365164.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAHNGAQA
                     ASVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVDNVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVEDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDD
                     ADYTNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGESKFVSTAGGVGLRHKF"
BASE COUNT          468 a          406 c          450 g          468 t
ORIGIN      
        1 tcggaaatgg ctgaaactca aaaaactaga ttcccacttt cgtgggaatg acggcagagc
       61 ggattctgtt gctcccgata aatgccgcaa cctcaaatcc cgtcattccc gcgaaggcgg
      121 gaatctagac attcaatgct aaggcaattt atcgggaatg actgaaactc aaaaaactgg
      181 attcccactt tcgtgggaat gacgggatta aagtttcaaa atttattcta aatagctgaa
      241 actcaacgca ctagattccc gcctgcgcgg gaatgacggc atattttgac attgaataaa
      301 aaagactaaa aacaggaaaa agccaaacag aaaacagcca aacagaaaac agcgaaacag
      361 aaaacagaaa aaagccaaac agaaaaaagc ctgtctggcg acaggctttt tgttgatacc
      421 aatctttgca gattagaatt tgtggcgcag accgacaccg ccggcagtcg atacgaattt
      481 gctttcgcct ttgccttctt gcaaccaacc ggcagaaacc aaggcagaag tgcgtttgga
      541 gaagtcgtat tccgcaccga caaccacttg gtcgtaatcg ttggtgtagt ctgcatcatc
      601 aaacgagcct ttgaagccgt gggcgtaaga aacgcggggc gttacgttgc cgaagcggta
      661 tgccaaggta gcggcaactt cggtttgaga gttgtgcgaa ttgtcttcaa ccagtttcgc
      721 gtcttgttgc tgtacggcta cggaagcgta tagggcatca ttgtcgtaac cgctgaccaa
      781 acggtgaatc tggtatttct caatcttcac gttatccaca tcctgatgtc ttttataggc
      841 accgccatat tgcacgaaga agccgccgtt tttgtagttg aagccggcat ggtaagattc
      901 gctgttatgt ctgcctgcat tgtcgttaag cgcgtattgt acgctgccgc tgaggccggc
      961 aaattcggga gaatcgtagc gtacggaaat gaggcgtgct tcgggttcgg caattttgtt
     1021 tacacccaaa tagtcgcttt tgctatccca aggattgatg tcgccggtgt ctttcaggac
     1081 gctgttcaaa cggccgacgc gcaatttacc gaagccgcct ttcaaaccga tgaaggattg
     1141 gcggttgccc caaccggagt cagtaccggc gatagatgcc ttttgctcaa cctgccaaat
     1201 ggctttcagg ccgttgccga ggtcttcttg gcctttgaag ccgattttcg aacccaaatc
     1261 aacgatgccg gtaccggttt caacgctagc cgcctgagct ccattgtgag ctacggagcg
     1321 ggaagtttct acgccggctt tgatggtgcc gtacagggta acgtcagcca ttgctgcaac
     1381 aggaagggct gccaaagtca gggcaatcag ggattttttc attgctgtat tccttttttg
     1441 gttaagaaat ttaagcaggc cgggctttcc aagccgctta gctttgcatt taccgccgac
     1501 gttttcggcg attccatgtc tgaaactata gatgttcctg tctgaaaaaa caaacttctg
     1561 cctgtttttg tgtggaattc gatgtgtatt ttgaagggcg gatagagata ttgtgggtat
     1621 tttttgtttt ataacatatg gttatttaaa ttttttaaga tttgcatttt tacaacactt
     1681 actcgggagg gtattggagg gcattgcaaa ccgagggtta taaagacggc aaaaaacgcg
     1741 cacgtggttt ctttcggaac gggcggatgc aaaacccgcc cctgcggcag ga
//
LOCUS       DAXHTY010000161         1738 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXHTY010000161 DAXHTY010000000
VERSION     DAXHTY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.161"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(26..349)
                     /locus_tag="ACFNMK_001985"
     CDS             complement(26..349)
                     /locus_tag="ACFNMK_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6365165.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(337..576)
                     /locus_tag="ACFNMK_001986"
     CDS             complement(337..576)
                     /locus_tag="ACFNMK_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HFC6365166.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(699..1496)
                     /gene="truA"
                     /locus_tag="ACFNMK_001987"
     CDS             complement(699..1496)
                     /gene="truA"
                     /locus_tag="ACFNMK_001987"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC6365167.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTIVAGRTDTGVHATAQVVHFDTAAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESVPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          400 a          410 c          494 g          434 t
ORIGIN      
        1 aacacaatcc attcagacta ttcaatcagg caaacatttc ctgcaatact gcaaacagtt
       61 tttcagccgt actgttgtct aaatttccaa gatgtttgac caatccggct ttatccacag
      121 ccctaatctg ttcgggcaaa agcaaaccgt ctttatcctg aaagcggaca ttgacgcgga
      181 acggggcagg acggcttccg ctcgtcatag gaacgatcag cacagtcttg agatagttgt
      241 gtatttcagg aggagagact acgacacaag gacgtgtctt tttgatttcg cttcctacgg
      301 tcgggtctaa ggagaccaga tagattccgc cgcgtactac catatccatt ctttatccgc
      361 ttcgttttca atttcggaaa aaaaatgctc ctgctcggtt tcgacaagca ttgcggcagc
      421 ttctgcccat cccctgcgaa cggtaggaca gcttaaaata atattgccct tttcaactgt
      481 aacagccaag ctgtctactg cccctatttg acccaataat gatttgggca gaatcacgcc
      541 ttgcgagttt cccattttgc gtatgttgag aatcatatac gtaccatatt caccagttta
      601 tgtaataaca atgttagtac cttgatgagg tagtgtcaac attgaaaaag attgcctgac
      661 agtttgtccg atttcaaaat ctccgcgaca agaatgtttt aaagccattc ggggatttgg
      721 gggcggatga tgccgtatgc ctcgggatag tcgacgccgg tcaggtaaag tccgtcgggc
      781 atgaaggtcg gcggggcttt gaggcggctg cgttcttgaa tcagtgcggc gaagccttcg
      841 acgctgagtc tgccgctgcc gacatagacg agcgcgccca tgatgttgcg taccatatgg
      901 tgcaaaaagg cgttgccgtg caaatcgagg cggacgagtc ctgagctttg ggtaaggtcg
      961 gcgcggtaga tggttttgac gggggatttt gcttggcatt cggcggcgcg gaagctggag
     1021 aagtcttgtt cgccgatcaa ataggcggca gcccgacgca tcggttcgat gtcgagtttg
     1081 aggtgtgtcc agcctgccct gtttttgagc aggggggaac ggacggggac ggattcgagc
     1141 aggtagcggt aatgccgtcc gtatgcgtca aatcgtgcat gaaattcggg ggcgacctgt
     1201 cgggcgtgca aaacggcaat gccttcgggc aggtgggcat ttacgccgcg cacccatgcc
     1261 tgttggggac gggcggcggc agtatcaaaa tgaacgactt gggcagtcgc atgaacgccg
     1321 gtgtcggtcc tgccggcaac aatggtggaa accgcttccc ctgctatttg ggcgagcgcg
     1381 gtttccaatg ccgcctgaac ggtcggtacg ccgtcagcct gtttctgcca gccgtaaaag
     1441 cggctgccgt cataggatag ggttattgcc cagcgttgtt tttgtgcggt atccatcgga
     1501 tttgggattc ggataaatgt tcagactgca ttgtatcgca gattttgcag ggaaacggca
     1561 aacgcccagg gcgagcggcg ttgtttgggg agttgggggg gtggatgcag ttgctacgaa
     1621 tcgctatcct gtgaattcac cctgtcagga gtgcccgaat cgtcattccc gcgcaggcgg
     1681 gaatctagga cgtaaaatct aaagaaacca ttttatagtg gattaacaaa aaccagta
//
LOCUS       DAXHTY010000162         1735 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXHTY010000162 DAXHTY010000000
VERSION     DAXHTY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.162"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            356..1660
                     /locus_tag="ACFNMK_001988"
     CDS             356..1660
                     /locus_tag="ACFNMK_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC6365168.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          357 a          433 c          512 g          433 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acaggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cggcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggga tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgcgta tttttaagcg ggatgcagat agacgctgcc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggagcgcatt gggtgtgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggttat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga taaac
//
LOCUS       DAXHTY010000163         1708 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXHTY010000163 DAXHTY010000000
VERSION     DAXHTY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.163"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            59..1432
                     /locus_tag="ACFNMK_001989"
     CDS             59..1432
                     /locus_tag="ACFNMK_001989"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6365169.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1612..>1708
                     /locus_tag="ACFNMK_001990"
     CDS             1612..>1708
                     /locus_tag="ACFNMK_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HFC6365170.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYSGLN"
BASE COUNT          442 a          557 c          408 g          301 t
ORIGIN      
        1 cccatcctat cggtttcccg cttcagacgg cataaggtct gaaagaaaga ctacaattat
       61 gagtaatcca ttttcctctt taggtttggg tacggaactc gtttccgcac tgaccgcgca
      121 aggttacgaa aacccgacgc ccatccaagc cgccgccatt cccaaagcac tcgccggtca
      181 tgatttacta gccgccgcgc aaaccggcac gggcaaaacc gccgccttta tgctgccctc
      241 gctcgaacgc ctcaaacgtt acgccaccgc cagcacctcg cccgcgatgc accccgtgcg
      301 tatgctcgtg ttgaccccca cgcgcgaact cgccgaccaa atcgaccaaa acgtgcagtc
      361 ctacatcaaa aacctgccgc tgcgccacac cgtcttgttc ggcggtatga atatggacaa
      421 acagaccgcc gatttgcgtg ccggctgcga aatcgtcgtc gccacagtcg gacggctgct
      481 cgaccacgtc aaacaaaaaa acatcagttt gaacaaagtc gaaatcgtcg ttctagacga
      541 agcagaccgc atgttggata tggggtttat cgacgacatc cgcaaaatca tgcagatgct
      601 gcccaaacaa cgccaaaccc tgctcttttc cgctaccttc tccgcaccga tacgcaaact
      661 ggcgcaagac ttcatgaacg cgcccgaaac cgtcgaagtc gccgcgcaaa acaccaccaa
      721 cgccaacgtc gaacaacaca tcatcgccgt cgataccatt cagaagcgca acctgctcga
      781 acggctgatt gtcgatttgc atatgaacca ggtcatcgtg ttctgcaaaa ccaaacaaag
      841 cgtcgaccgc gtaacgcgcg aactggtgcg ccgcaacctg tccgcacagg cgatacacgg
      901 cgaccgttcc caacaaagcc ggctcgaaac actcaacgcc ttcaaagacg gcagcctgcg
      961 cgtcctcgtc gccaccgaca tcgccgcgcg cgggctggac attgccgaac tgcccttcgt
     1021 catcaattac gaaatgcccg cccagcccga agactacgtc caccgcatcg ggcgcacggg
     1081 gcgcgcgggc gcggacggcg tggcgatttc cctgatggac gaatccgaac agaaaatgtt
     1141 tgaatccatt aaagagctga ccggcaacaa gctgctcatc gagcgcatcg agggcttcga
     1201 gccgcaatgg tgggaacagg gcggcgcaaa accggaaaaa accgaaatgc gcgaaccgag
     1261 acaacgcaac cgctacgaat ccgccagggc tcagcgcgaa aaaaataccc gaccggaaac
     1321 cacggcaaac gatgcgggcg cggcttgcgg aaaaattgcc ggacgcagcc gccgaagccg
     1381 tcgggaacac cggacgtgcg ccctgctcca accgcgttac ggcgtaaaat aaccctgaaa
     1441 atcaaatgcc gtctgaacat ttcccgtttc agacggcatt tttcaaaccg gactgacgca
     1501 tcgggagcaa ccgcccgcac cggataaatt tcttccgcaa acagtttcag acggcatttg
     1561 ctgcctgtac aatatcattt ccttttccac tcaaaccatc aggatttccc aatgtccacc
     1621 caattcgatg tcgccgtaat cggcgcaggt ccggcaggct cggtcgcatc cgccctgctc
     1681 cgcaaaaaag gttatagtgg attaaatt
//
LOCUS       DAXHTY010000164         1671 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXHTY010000164 DAXHTY010000000
VERSION     DAXHTY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.164"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            225..>1671
                     /gene="iga"
                     /locus_tag="ACFNMK_001991"
     CDS             225..>1671
                     /gene="iga"
                     /locus_tag="ACFNMK_001991"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC6365171.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLANNERDANNGQNVTFEDNGTLVLDQNINQGAGGLFFKGDYTVKGINN
                     DITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSSNQ"
BASE COUNT          591 a          353 c          341 g          386 t
ORIGIN      
        1 acaaaaagcc gcagaaattt aacggaagaa ccgaaccccg aaaatcggct tcccgccttc
       61 gcttgaataa cagaacaaag gaagcggaaa taaaaacatc gggattcttt ttttaaattg
      121 gcaataaaac accaaaatga ataattcaca caacaaaaaa tagtcaacac cgccaaaccg
      181 gcgtaaaata caaaacatta tcacccttaa aacggtaaaa ccttatgaaa accaaacgtt
      241 ttaaaattaa cgccatatcc ttatccatct ttcttgccta tgcccttaca ccatactcag
      301 aagcggcatt ggtcagagac gatgtcgatt atcaaatatt ccgtgacttt gcagaaaaca
      361 aaggcaaatt ttttgtcggc gcaaccgatt tatcagtgaa aaacaaacaa ggtcaaaaca
      421 tcggcaacgc attatccaac gtaccgatga ttgattttag cgttgcagat gtcaacagac
      481 gtacattaac cgtcatcgat ccccagtatg ccgtcagcgt caaacatgta aaaggagacg
      541 aaatctctta ttacgggcat cacaatgggc acttagatgt tagcaatgat gaaaacgaat
      601 atcgttcagt tgcacaaaat gactacgaac caaataaaaa ttggcatcac ggtaatcaag
      661 gtcgtcttga agactacaac atggcacgcc ttaataaatt cgtaacagaa gtcgcaccta
      721 ttgcaccaac cagtgcaggc ggcggcgttg aaacctataa agataaaaac cgtttttctg
      781 agtttgtgcg agttggagca ggtacgcaat ttgaatataa cagccgctac aatatgacag
      841 agctttcacg agcttatcgt tatgccattg caggtacgcc ttatcaagac gtgaatgtca
      901 catcgaatct aaatcaagaa ggcttgattg gcttcggtga taattcaaaa catcattccc
      961 cagaaaaact caaagaagtt ttatctcaaa acgcattgac taactatgct gtgttaggcg
     1021 atagcggttc accattattt gcttatgata aacaagaaaa acgctgggtc tttttaggtg
     1081 cttacgatta ttgggcaggc tatcaaaaaa actcttggca agaatggaat atctataaaa
     1141 aagaatttgc agataaaatc aaacaacgcg ataacgccgg caccatcaaa ggttatggcg
     1201 aacatcattg gaaaaccacg ggcacaaaca gccatatcgg ttcgacagcg gtaaggcttg
     1261 ccaacaatga aagggatgca aacaacggac agaatgttac ctttgaagac aacggcactt
     1321 tggtattgga tcaaaacatc aaccaaggcg cgggcggtct gtttttcaaa ggcgattaca
     1381 cagtcaaagg tataaataat gacatcactt ggctaggtgc ggggattgat gttgccgacg
     1441 gcaaaaaagt cgtttggcaa gtcaaaaatc cgaatggcga cagattggca aaaatcggca
     1501 aaggcacatt agaaataaac ggcacaggcg ttaaccaagg gcaattaaaa gtcggcgatg
     1561 gtacggttat tctgaatcaa caagccgatg ccgacaaaaa agtccaggct ttctcccaag
     1621 tcggcattgt cagcggacgc ggtacattgg tattaaatag ttcaaatcag a
//
LOCUS       DAXHTY010000165         1667 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXHTY010000165 DAXHTY010000000
VERSION     DAXHTY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.165"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..533
                     /gene="mafB"
                     /locus_tag="ACFNMK_001992"
     CDS             <1..533
                     /gene="mafB"
                     /locus_tag="ACFNMK_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6365172.1"
                     /translation="DQWMQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDF
                     SDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISGHRNYIEGKST
                     LNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLSTNFGTIHSGKN
                     GVHIVPANPKTIKKVQ"
     gene            536..853
                     /locus_tag="ACFNMK_001993"
     CDS             536..853
                     /locus_tag="ACFNMK_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365173.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            1017..1663
                     /locus_tag="ACFNMK_001994"
                     /pseudo
     CDS             1017..1663
                     /locus_tag="ACFNMK_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
BASE COUNT          575 a          323 c          363 g          406 t
ORIGIN      
        1 ttgaccagtg gatgcaggaa aaccccaatg ccgccgaaac cgtcgaagcc ctggtcaacg
       61 tcctgccgtt tgccaaagtc aaaaacctga caaaggcggc aaaaccgggg aaggctgcgg
      121 ttagtgggga tttctcagac tcctacaagc ataacactgc ttcaagatta tctcagtctg
      181 tagatggaga aatgtttcaa acccgcaatg ttgattttaa agcaaaatct attgggacta
      241 aaattcatga tggagctcaa gggaaacata tttcaggaca tagaaactac attgaaggta
      301 agagtacttt aaatcaaaac attaatcctc aagaattgtt gaacggaata cattcaggtg
      361 cttatccagt tatttctaaa ggagcaagag gaaatcctgt tgttgatttt gggtatccta
      421 taggcagcga tgggaaatca ggattaagta ccaattttgg tacgattcat tcaggtaaaa
      481 atggagttca cattgttccg gctaacccta aaaccattaa aaaggtgcaa tagttatgaa
      541 tatattacca agctggctgc gagtcggtat gaatatagca atgctgggca tgatacactc
      601 agatatcagg ttaattaccg tagattacga ggaaggaaga aggtttttaa aaatcaaaaa
      661 ttatttatca agagaagcca tcacagaaga ccatgaagat atggaatatt tgattacaga
      721 gttatggtct atgtgtggag aatattttga tgaagctgac tttgaatgta tttattctaa
      781 tcattcttct atggagttaa accaaataaa tggtgcagta ttcaggagaa aggaattaat
      841 ttcgcaagcg taggttaaaa aaccaacaat cacaatgtct tctgaaaccg tgtttaattt
      901 tcagacggca tttccttcgt ttgaaatagg atattgagaa ctgagttctt caaaaatcct
      961 acacttgctc cttccacggc agcaccttgg tcaaaacggc agacggctga aaagccattg
     1021 cccgtatccg aaccggcgac cgcgtcttcg ccaaggacga ggcaagcgga aaaacgggat
     1081 acaaacccgt taccgcccga tacggcaatc cgtatcaaga aaccgtttac attgaaattt
     1141 cagacggcat cggtaaaata cagaccctcg tttccaataa aatccacccg ttttacagtc
     1201 aaggaaagtg gatacaggca ggtcgtctga aaaaaggcga caccctgctt tccgaaagcg
     1261 gtgcaaaaca gacggttcaa aacattaccc tcaaacagca gccgctcaaa gcctacaatc
     1321 tgaccgttgc cgactggcat acctacttcg tcaagggtaa tcaggcggaa cggaaggggt
     1381 ttgggttcat aatgcgtgtc cgccaagaaa aacaccatct acccccgtat atgggaatga
     1441 ttcagaagca tatgcagcag caaagaaatt gggatataga aaaataaaag aaagaacgag
     1501 aaatgatgct gctattttta aaaaaggaaa gagttatata tcacgcgatg tagattcaca
     1561 taatggaggg gcttggaaag aagcatcttc acccaagaag ctgaacagaa aagaaacaag
     1621 aaacggtaca tttgataaaa acctaaatcg tattggagat taattat
//
LOCUS       DAXHTY010000166         1664 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXHTY010000166 DAXHTY010000000
VERSION     DAXHTY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.166"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(190..588)
                     /locus_tag="ACFNMK_001995"
     CDS             complement(190..588)
                     /locus_tag="ACFNMK_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365174.1"
                     /translation="MCKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFID
                     NIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDFF
                     EKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(617..967)
                     /locus_tag="ACFNMK_001996"
     CDS             complement(617..967)
                     /locus_tag="ACFNMK_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365175.1"
                     /translation="MPSETVFNFQATFPLFETGYWELSSSKILHLLLPRQHLGQNGRR
                     LKSKHRPSCCRLRMSTGQPQCRRNRRSRLQHCRRQSRKVGKNGKTGEIKADGRKVNVR
                     IDSTEADLLYPAGQ"
     gene            complement(986..1363)
                     /locus_tag="ACFNMK_001997"
     CDS             complement(986..1363)
                     /locus_tag="ACFNMK_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC6365176.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRRKYPYPMDIPRDIVIGISTIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            complement(1394..>1664)
                     /locus_tag="ACFNMK_001998"
     CDS             complement(1394..>1664)
                     /locus_tag="ACFNMK_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm41 family immunity protein"
                     /protein_id="HFC6365177.1"
                     /translation="LIEVRRKYPYPMDIPRDIVIGIGSIIDFLMVQNWKLFEIKASPW
                     LPKSVKINERYERFRVMLRYIFTDLDVDDWKFFYFPIQHSKGRLR"
BASE COUNT          487 a          339 c          254 g          584 t
ORIGIN      
        1 ttccacataa ttatttacct ttatccattg cttcccaacc gatacatccg gtattttagc
       61 ttctcctttt ccgtcaaaca ctcctttggt tacctgcttt gtgtcaaaaa taccgtctga
      121 aagccgaata ttgtttcaga cggcattttg attgttgggt ttttaatcag agctttgttt
      181 gataatttgt tattttgaat aaaatacagg ttctgtcgag ctgataactt ctattacaga
      241 atcgctaaaa aagaaaatat aagccttata atatttcttt tcaaaaaaat caaaactttc
      301 ttctttcaac cagtccatat aactgtcatc tattatctcg aaaaaataat ttatagcagg
      361 atttctatcc caatcatttt tttctttatt ttcaaataaa tattctgaaa attttacttt
      421 ataagattca tctgttattt gaaaatgaat tatattgtca ataaatattt caatatttaa
      481 ttcataattt cggtcggatg ccaaaatgtt cacatcttgt tcggtaacac aaattttgtc
      541 cacataaaat ctgtgattat tgttagaaaa ttgtaatggt ttacacaatc gttttccata
      601 tctaattttt gttttcttat tgccctgccg gataaagcag gtctgcctcc gtactgtcta
      661 tcctcacatt tactttcctg ccatcggctt ttatctcccc ggttttaccg tttttgccaa
      721 ctttgcggct ttggcggcgg caatgttgaa ggcggcttcg acggtttcgg cggcattggg
      781 gttgacccgt actcatccgc aaacggcaac acgacggacg gtgtttgctt ttcagccgtc
      841 tgccgttttg accaaggtgc tgccgtggaa ggagcaggtg taggattttt gaagaactta
      901 gttcccaata tcctgtttca aataagggga aggtcgcctg aaaattaaac acggtttcag
      961 acggcatttt gattgccggg gtttgctatt ttttgttgta ataatcaaat cgcacgttga
     1021 ctatgtcttt ctcggtaaaa atataacgga gcattgttgt gaatctttca taacgttcat
     1081 gaattcccac gctatcaggt agccaagggg aagctttaat ttcgaaaagc tcccaatttg
     1141 gaaccattaa aaaatcaata atggtactga ttccaatcac aatatccctt ggtatatcca
     1201 tcggataagg atattttctc ctaacctcga ttaaagcatt ctccaatttc caatattctt
     1261 catcatccca caccccgtca tcataccatt tgccaataaa tgaattttcg tcatactcct
     1321 taaaacaagg gatgtttctt ctaaaatcct tgaactcgca cataataatg tatctccaat
     1381 ataattaaac ttttcatctc aatctacctt tactatgttg tattggaaag taaaaaaatt
     1441 tccagtcatc tacatctaga tcagtaaaaa tataacggag cattactctg aacctttcat
     1501 aacgctcatt aattttgaca cttttaggca accaagggga agctttaatt tcaaaaagtt
     1561 tccaattttg aaccattaaa aaatcaataa tgctacctat tccaatcacg atgtcccttg
     1621 gtatatccat cggataagga tattttctcc taacctcgat taaa
//
LOCUS       DAXHTY010000167         1593 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXHTY010000167 DAXHTY010000000
VERSION     DAXHTY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.167"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..876
                     /gene="mafA"
                     /locus_tag="ACFNMK_001999"
     CDS             <1..876
                     /gene="mafA"
                     /locus_tag="ACFNMK_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6365178.1"
                     /translation="AHGGGKRFAVEQELVAASSRAAVKEMDLSALKGRKAALYVSVMG
                     DQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPAYDTTATTKSDALSSVTTSTSLL
                     NAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLANPRDVSFLTNLIQTVFYLRGIE
                     VVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLKAQTKLEYFAVDRDSRKLLIAPK
                     TAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFSDITPYGDTTAQNRPDFKQNNGK
                     NPDVGNEVIRRRKGG"
     gene            880..>1593
                     /gene="mafB"
                     /locus_tag="ACFNMK_002000"
     CDS             880..>1593
                     /gene="mafB"
                     /locus_tag="ACFNMK_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6365179.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNP"
BASE COUNT          419 a          596 c          355 g          223 t
ORIGIN      
        1 gcccacggcg gcggcaaacg ctttgccgtc gaacaagaac tcgtcgccgc atcgtcccgc
       61 gccgccgtca aagaaatgga cttgtccgcc ctgaaaggac gcaaagccgc cctttacgtc
      121 tccgttatgg gcgaccaagg ttcgggcaac ataagcggcg gacgctactc tatcgacgca
      181 ctgatacgcg gcggctacca caacaacccc gaaagtgcca cccaatacag ctaccccgcc
      241 tacgacacta ccgccaccac caaatccgac gctctctcca gcgtaaccac ttccacatcg
      301 cttttgaacg cccccgccgc cgccctgacg aaaaacagcg gacgcaaagg cgaacgctcc
      361 gccggactgt ccgtcaacgg cacgggcgac taccgcaacg aaaccctgct cgccaacccc
      421 cgcgacgttt ccttcctgac caacctcatc caaaccgtct tctacctgcg cggcatcgaa
      481 gtcgtaccgc ccgaatacgc cgacaccgac gtattcgtaa ccgtcgacgt attcggcacc
      541 gtccgcagcc gcaccgaact gcacctctac aacgccgaaa cccttaaagc ccaaaccaag
      601 ctcgaatatt tcgccgttga ccgcgacagc cggaaactgc tgattgcccc taaaaccgcc
      661 gcctacgaat cccaatacca agaacaatac gccctctgga tgggacctta cagcgtcggc
      721 aaaaccgtca aagcctcaga ccgcctgatg gtcgatttct ccgacatcac cccctacggt
      781 gacacaaccg cccaaaaccg tcccgacttc aaacaaaaca acggtaaaaa ccccgatgtc
      841 ggcaacgaag tcatccgccg ccgcaaagga ggataaaccg tgaaaccgct gcgcagactg
      901 acaaacctcc ttgccgcctg cgccgtagcg gccgccgcct tcggacagcc cgccctcgcg
      961 gcggacttgg cgcaagaccc gttcattacc gataacgccc aacggcagca ctacgaaccc
     1021 ggaggcaaat accacctctt cggcgacccg cgcggcagcg tttccgaccg caccggtcaa
     1081 atcaacgtca tccaagacta tacccaccag atgggcaacc tgctcatcca acaggcaaac
     1141 atcaacggca caatcggcta ccacacccgc ttttccggac acggacacga agaacacgcc
     1201 cccttcgaca accacgccgc cgacagcgca agcaaagaac aaggcaacgt tgacgacggc
     1261 tttaccgtgt accggctcaa ctgggaagga cacgaacatc atcccgccga tgcctacgac
     1321 ggcccgaagg gcggcaatta ccccaaacct acgggcgcac gagacgaata cacctatcac
     1381 gtcaacggca cagcccgcag tatcaaactc aatccgaccg acacccgcag catccggcaa
     1441 cgcatatccg acaactacaa caacctcggc agcaatttct ccgaccgcgc cgatgaagcc
     1501 aacagaaaaa tgttcgagca caatgccaag ctcgaccgct ggggcaacag catggagttt
     1561 atcaacggcg tcgccgccgg cgcgctcaac ccc
//
LOCUS       DAXHTY010000168         1553 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXHTY010000168 DAXHTY010000000
VERSION     DAXHTY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.168"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            476..1297
                     /locus_tag="ACFNMK_002001"
     CDS             476..1297
                     /locus_tag="ACFNMK_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6365180.1"
                     /translation="MQPPKNLLFSSLLFSSLLFSSLLFSSLLFSSAAQAASEDGSRSP
                     YYVQADLAYAAERITHNYPEPTGTDKDKISTVSDYFRNIRAHSIHPRVSVGYDFGDWR
                     IAADYASYRKWNNNKYSVNTKEVRRNGGNSENLKTENQENGSFHAVSSLGLSAIYDFK
                     LNDKFKPYIGARVAYGHVKHQVHSVEKETTIYNTAPTKGAKKGGLIVSAQPSKPPYHE
                     SRSISSLGLGVIAGVGFDITPNLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYRF"
BASE COUNT          436 a          434 c          313 g          370 t
ORIGIN      
        1 taatctttga atattgctgt tgttctaaag tctagattcc cgcctgcgcg ggaatgacga
       61 ttcataagtt tcccgaaatt ccaacataac cgaaacctga cagtagccgt agcaactgaa
      121 ccgtcattcc cgcaaaagcg ggaatctaga aatgaaaagc aacaggcatt tatcggaaat
      181 aactgaaacc gaacagacta gattcccgcc tgcgcgggaa tgacggctgc agatgcccga
      241 cggtctttat agtggattaa caaaaatcag gacaaggcga cgaagctgca gacagtacag
      301 atagtacgga atcgattcac ttggtgcttc agcaccttag agaatcgttc tcttttttgt
      361 tcatccgcta tattgtgttg aaacatcgcc ccaaacctga tatagtccgc tcctgcaaca
      421 tcattgaaaa tctttctttt taatcagtta aaaccgaata cggagtcgaa aatgaatcca
      481 gcccccaaaa aaccttctct tctcttctct tctcttctct tctcttctct tctcttctct
      541 tctcttctct tctcttctct tctcttccgc agcgcaggcg gcaagtgaag acggcagccg
      601 cagcccgtat tatgtgcagg cggatttagc ctacgccgcc gaacgcatta cccacaatta
      661 tccggaacca accggtacag acaaagacaa aataagcacg gtaagcgatt atttcagaaa
      721 catccgtgcg cattccatcc acccccgagt gtcagtcggc tacgatttcg gcgactggag
      781 gatagcggca gattatgcca gttacagaaa atggaacaac aataaatatt ccgtcaacac
      841 aaaagaggtg agaagaaacg ggggcaacag tgaaaacctg aagacggaaa atcaggaaaa
      901 cggcagcttc cacgccgttt cttctctcgg cttatccgcc atttacgatt tcaaactcaa
      961 cgataaattc aaaccctata tcggcgcgcg cgtcgcctac ggacacgtta aacatcaggt
     1021 tcattcggtg gaaaaagaaa ccactattta taacactgca ccaacgaaag gcgctaaaaa
     1081 gggaggcttg attgtatccg ctcagcccag caaacctccc tatcacgaaa gccgcagcat
     1141 cagcagcttg ggtcttggcg ttatcgccgg tgtcggtttc gacatcacgc ccaatctgac
     1201 tttagacacc ggataccgct accacaactg gggacgcttg gaaaacaccc gcttcaaaac
     1261 ccacgaagtc tcattgggca tgcgctaccg cttctgattc cccgacaccg atgccgtctg
     1321 aaccttcaga cggcattttt tacacaattc ccaccgtttc ccatcattcc cgatacaccg
     1381 taatctcgaa acccgtcatt cccgcgcagg cgggaatcca gacctgtccg cacagaaact
     1441 tatcggataa aacggtttct ttagatttta cgtcctagat tcccacttcc gtgggaatga
     1501 cggttcagtt gcattcccga caacaacgca atctcgaaac ccgtcattcc tgc
//
LOCUS       DAXHTY010000169         1529 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXHTY010000169 DAXHTY010000000
VERSION     DAXHTY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.169"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            176..394
                     /locus_tag="ACFNMK_002002"
     CDS             176..394
                     /locus_tag="ACFNMK_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC6365181.1"
                     /translation="MKFINTCRKYGAKLAVVTAAPLALAAHANTTLPDTAKNALEAAK
                     ADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            401..679
                     /locus_tag="ACFNMK_002003"
     CDS             401..679
                     /locus_tag="ACFNMK_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365182.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGVMASVYFVSLLKKVLK"
     gene            807..1124
                     /locus_tag="ACFNMK_002004"
     CDS             807..1124
                     /locus_tag="ACFNMK_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC6365183.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
     gene            1108..1503
                     /locus_tag="ACFNMK_002005"
     CDS             1108..1503
                     /locus_tag="ACFNMK_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6365184.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDPETDKFAKVSG"
BASE COUNT          463 a          302 c          350 g          414 t
ORIGIN      
        1 aatgccgatt atttctacga cttcgaagaa gcgattaacg caggtttgga agaaatcggc
       61 aaccaatacg aatttgtcgt attcggattt ttgaaagact gattttcgga tgttcggcgg
      121 tcgtttgaaa aacgctccat ccattaccgc caaacacttt ttgaaggaaa atatcatgaa
      181 atttattaac acctgccgta aatacggcgc aaaactggct gttgtaacag ctgctcccct
      241 ggctttggcc gcacatgcaa atacaacgtt gcccgatacg gcaaaaaacg ctttggaagc
      301 cgcaaaagcg gacggtatgg aagccggttg gattgtagtg ggcgttttcg ccgcgctttt
      361 tgtgttttcc atcgttaaga gagtgatgaa gtaagacggc atgtactacc aagtcggaaa
      421 taaatgtctt gagaagcacc aggctgaaaa cctttatttc agcttggtag taccaagaat
      481 caaagaaaac ggacagattg tcaggccgga atataacggc agcctgtgga agatgtcgga
      541 cggtcagccg ctaaggcttt tattggcgga atgcagtccg aaagacaacc tgcaaagcgg
      601 tcttgaaaca ggctggatag tattcggcgt gatggcatcc gtttactttg tttccctgct
      661 gaaaaaggtt ttgaaatgat ggatttttat ttttatctcg gcgtttccgt acccgtatta
      721 atcggggcgg ttctgtttaa gaattgagcg catgaagtta tggtgtcaaa atcaggcttt
      781 caaaacaacc tttgaaaggc agaaccatga acaagccgtt tatcactcag gcgcagttgg
      841 cactttataa atatcagccg tcaagcaagt attttgggca atcgatggcg gttatagcgc
      901 aatctgaatt tgttgaattt gcgaagatta ataagtctga aaatgttatt gattgtttct
      961 cttttttctg gaatagaaga attaaacatg atatttggct aatctcattt tctgataatt
     1021 cagaaatggt aattaaagaa tccctgaaag atggtcataa aatatacaaa tttgaatttt
     1081 gcgaaattgt cgataattgc aattttgatg atgtattcgt ttgaagcgaa tgcaaatgca
     1141 gtaaaaatat ctgaaactgt ttcagttgat accggacaag gtgcgaaaat tcataagttt
     1201 gtacctaaaa atagtaaaac ttattcatct gatttaataa aaacggtaga tttaacacac
     1261 atccctacgg gcgcaaaagc ccgaatcaac gccaaaataa ccgccagcat ttcccgcgcc
     1321 ggcgtattgg cggggggcgg caaacttgcc cgcttaggcg cgaaattcag cacaagggcg
     1381 gttccctatg tcggaacagc ccttttagcc catgacgtat acgaaacttt caaagaagac
     1441 atacaggcac gaggctacca atacgacccc gaaaccgaca aatttgcaaa ggtctcaggc
     1501 taagtgcgcc tgttgccgcc taaaaggta
//
LOCUS       DAXHTY010000170         1441 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXHTY010000170 DAXHTY010000000
VERSION     DAXHTY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.170"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            27..1364
                     /locus_tag="ACFNMK_002006"
     CDS             27..1364
                     /locus_tag="ACFNMK_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6365185.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRALRPGTQWLWVGRMGVAACFVLLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYLISAVIMPVGALSVA
                     VFTAWIQDRQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          263 a          338 c          391 g          449 t
ORIGIN      
        1 tatcttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtgct tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
      121 atacggcagg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttaccgtct
      181 tggtcgccct gcccgtccag cttgccgagt tttatatcgg gcgcacaggc ggtaaaaatg
      241 ccgtcgattc cttcagggct ctgcgtccgg gtacgcaatg gctttgggtc gggcgcatgg
      301 gcgttgccgc ctgctttgtt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattga
      361 attatgtcgt ccacagtttt acgggggcaa tccatgcggg agcggacttt gaagccctgt
      421 tcggcacaac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
      481 ttacggtttg ggtggtcaaa ggcgggattt cagacggcat tgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttggga caggcgtttt ttgccttgag catcggcgtt tccgctatga
      721 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgctcgggg catacgatta
      781 tgtggatgaa cctcttggtt tcgctgttgg caggtttggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg cccgcagtgt
      901 ttatgaagat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcattt tcgatgttgg aaacggtcat tgccgcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa catacttggc ttatcggcac ggctattttc attgtcggca
     1081 tcccatccgc gctgtctttc ggcgtatggg gtgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactacctt atttctgcag tcatcatgcc tgtcggcgca ttgagcgttg
     1201 ccgtatttac cgcttggatt caggacaggc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ttgctgctgt ggctgaatac cttgcgctac ctcgccccga
     1321 ttgccattat tattgttttc atcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaagcc tttcagacgg catttttgct tcaggttcag cctatttcgt
     1441 t
//
LOCUS       DAXHTY010000171         1427 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXHTY010000171 DAXHTY010000000
VERSION     DAXHTY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.171"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            53..1234
                     /locus_tag="ACFNMK_002007"
     CDS             53..1234
                     /locus_tag="ACFNMK_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFC6365186.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQAQLTEQL
                     QVTNGVQGNQVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGG
                     GASQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAINPWDSNNDVASQLGIFKRHDD
                     MPVSVRYDSPEFSGFSGSVQFVPAQNSKSAYKPAYYTKDTNNNLTLVPAVVGKPGSDV
                     YYAGLNYKNGGFAGNYAFKYARHANVGRNAFELFLIGSATSDEAKGTDPLKNHQVHRL
                     TGGYEEGGLNLALAAQLDLSENGDKAKTKNSTTEIAATASYRFGNAVPRISYAHGFDL
                     IERGKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHK
                     F"
BASE COUNT          367 a          352 c          365 g          343 t
ORIGIN      
        1 gtgtttgccc gatgttttta ggtttttatc aaatttacaa aaggaagccg atatgcgaaa
       61 aaaacttacc gccctcgtat tgtccgcact gccgcttgcg gccgttgccg atgtcagcct
      121 gtacggcgaa atcaaagccg gcgtggaagg caggaacatc caggcgcaat tgaccgagca
      181 gctccaagta actaacggtg tgcaaggcaa tcaggtaaaa gttactaagg ccaaaagccg
      241 catcaggacg aaaatcagcg atttcggctc gtttatcggc tttaagggga gcgaggattt
      301 gggcgaaggg ctgaaggctg tttggcagct tgagcaagac gtatccgttg ccggcggcgg
      361 cgcgtcccag tggggcaaca gggaatcctt tatcggcttg gcaggcgaat tcggtacgct
      421 gcgcgccggt cgcgttgcaa atcagtttga cgatgccagc caagccatta atccttggga
      481 cagcaataat gatgtggctt cgcaattggg tattttcaaa cgccacgacg atatgccggt
      541 ttccgtacgc tacgattctc cggaattttc cggtttcagc ggcagcgtcc aattcgttcc
      601 ggctcaaaac agcaagtccg cctataagcc ggcttattat actaaggata caaacaataa
      661 tcttactctc gttccggctg ttgtcggcaa gcccggatcg gatgtgtatt atgccggtct
      721 gaattacaaa aatggcggtt ttgccgggaa ctatgccttt aaatatgcga gacacgccaa
      781 tgtcggacgt aatgcttttg agttgttctt gatcggcagc gcgacgagtg atgaagccaa
      841 aggtaccgat cccttgaaaa accatcaggt acaccgcctg acgggcggct atgaggaagg
      901 cggcttgaat ctcgccttgg cggcccagtt ggatttgtct gaaaatggcg acaaagccaa
      961 aaccaaaaac agtacgaccg aaattgccgc gactgcttcc taccgcttcg gtaatgcagt
     1021 tccacgcatc agctatgccc atggtttcga cttgatcgaa cgcggtaaaa aaggcgaaaa
     1081 taccagctac gatcaaatca tcgccggcgt tgattatgat ttttccaaac gcacttccgc
     1141 catcgtgtct ggcgcttggc tgaaacgcaa taccggcatc ggcaactaca ctcaaattaa
     1201 tgccgcctcc gtcggtttgc gccacaaatt ctaaatatcg gggcggtgaa gcggatagct
     1261 ttgtttttga cggcttgcct tcattctttg attgcaatct gactgccaat ctgcttcagc
     1321 cccaaacaaa aatccggata cggaagaaaa acggcaataa agacagcaaa taccgtctga
     1381 aagattttca gacggtattt cgcatttcgg cttggtttgc acatata
//
LOCUS       DAXHTY010000172         1416 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXHTY010000172 DAXHTY010000000
VERSION     DAXHTY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.172"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            188..1372
                     /gene="ubiM"
                     /locus_tag="ACFNMK_002008"
     CDS             188..1372
                     /gene="ubiM"
                     /locus_tag="ACFNMK_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC6365187.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKARLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT          378 a          413 c          307 g          318 t
ORIGIN      
        1 ataccccaca cccgatttga tacacattaa gctgaaagta aaaatccgca tacaccctcc
       61 cctgcatatt tcttcaacag tgggttttga tataatcgcc tatctgttac agatagttca
      121 aacggcattc aaacccttac aaataccgtc tgaaatcatc ggcatccgtc tatgcaaagg
      181 atattttatg agtttacaca gcgacatcct cgtcgtcggc gcaggtccgg cgggtttaag
      241 ttttgcagcc gaacttgccg gcagcggttt gaaagttacc ctgatcgaac gcagcccact
      301 gactgttctg caaaatcctc catatgacgg acgcgaaatc gccctgaccc atttttcccg
      361 cgaaatcatg cagcgtctgg gtatgtggga caaaattccc gaaaatgaaa tttacccttt
      421 gcgcgatgcc aaagtgctga acggacgttc cgactaccag ctccactttc cccaaccgac
      481 cgaggcgcgc ggcgaacctg ccgactgttt gggctatctt atttccaacc acaatatccg
      541 acgcgctgcc tacgaagtcg tatcccaact cgacaacatt accattctga ccgatacggt
      601 tgtcaaagaa gtcaaaacat ctgacaacga agcacaagtc attttggaaa acggcaaaat
      661 cctgactgca cgcctgcttt tggcggcaga cagccgcttc tcgcaaaccc gtcgacaact
      721 cggtatctcc tccgatatgc acgattacag ccgaaccatg ttcgtctgcc gtatgaaaca
      781 taccctttcc aaccaacata ccgcttacga atgcttccat tacggacgca ccatcgcact
      841 gctgccgctg gaagagcacc tgaccaatac cgtcattacc gtcgataccg ataaaatcaa
      901 cagcgttcaa aacctttcgc ccgaagaatt ggcggcaagc gtaaaagaac agctcaaggc
      961 caggctcggc gatatggaac tggtcagcag catccaccat tacccccttg tcggtatgat
     1021 tgccaaacgt ttctacggca aacgcagcgc gctgatcggc gatgcggctg tcggtatgca
     1081 ccccgttacc gcacacggtt tcaacttggg tctgtccagc gcagatattt tggcaaaact
     1141 gatactcgaa gccgagcagc gcggtcagga catcggcgcg gcttccttgt tggaaaaata
     1201 cagcagcaaa catatgctgc acgcccatcc gctttatcac gggaccaata tgatgctgaa
     1261 gctgtttacc aacgaaaccg caccggcaaa actgctgcgc ggcttggtat tgcgtgccgg
     1321 caacaatttc ccgccgctga aaaagctgat taccaaacaa ctgaccggtt gaaaactgcc
     1381 gaaatgccgt ctgaaacgtt tcagacggca tttttg
//
LOCUS       DAXHTY010000173         1407 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXHTY010000173 DAXHTY010000000
VERSION     DAXHTY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.173"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            110..559
                     /locus_tag="ACFNMK_002009"
     CDS             110..559
                     /locus_tag="ACFNMK_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6365188.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLFK"
     gene            complement(649..1043)
                     /locus_tag="ACFNMK_002010"
                     /pseudo
     CDS             complement(649..1043)
                     /locus_tag="ACFNMK_002010"
                     /inference="COORDINATES: protein motif:HMM:NF017953.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            1256..>1407
                     /locus_tag="ACFNMK_002011"
     CDS             1256..>1407
                     /locus_tag="ACFNMK_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6365189.1"
                     /translation="MSTFFQQTAQAMIAKHINRFPLLKLDRVIDWQPIEQYLNRQKTR
                     YLRDHRG"
BASE COUNT          421 a          239 c          301 g          446 t
ORIGIN      
        1 tataccggcc gctaaaaaat agagtgggaa atatgcatac tgttgaatgg gatagtaagt
       61 ctgttcaaag aaatatgttg aataatctgt tcttattgga agtaaagtaa tgactgataa
      121 tcgggggttt acgctggttg aattaatatc agtggtcttg atattgtctg tacttgcttt
      181 aattgtttat ccgagctatc gcaattatgt tgagaaagca aagataaatg cagtgcgggc
      241 agccttgtta gaaaatgcac attttatgga aaagttttat ctgcagaatg ggaggtttaa
      301 acaaacatct accaaatggc caagtttgcc gattaaagag gcagaaggct tttgtatccg
      361 tttgaatgga atcgcgcgcg gggctttaga cagtaaattc atgttgaagg cggtagccat
      421 agataaagat aaaaatcctt ttattattaa gatgaatgaa aatctagtaa cctttatttg
      481 caagaagtcc gccagttcgt gtagtgacgg gctggattat tttaaaggaa atgataagga
      541 ctgcaagtta tttaagtagg cttgtttaca aatcgatcga tagtgtttca gtatttgtgc
      601 ggttttccgc cttgatggaa tgaaaatcgt gtaatattaa gcctatagtt atgactcgta
      661 atctgtatct ggattgtttc tggataaaag aataaaccca atcgttgggt cgtttgggtt
      721 tatttcattg attgacagaa gttttttaat aaatggaaag acaacgcctt ttttagggga
      781 tggttttgtt tttccctctg atattgccta taggtttctg ccccttccat tctaaatata
      841 ttatccgatt cggataagtc gaatagggca cttgatgcta agccgattac aagtcgtttc
      901 gataaatcat aggacataat aaggatatct ttagccgtgg atttatgggg catagtagca
      961 aaaattgtat aagggtgtac attactcatc tgaaaaggtg aacagatttt ataatgccgt
     1021 ctgaagatgt tttcagacgg cattttgttt ccaattcgtt ataggcttgt attttgccta
     1081 tgcaatatat agtggattaa atttaaacca gtacagcgtt gcctcgcctt gtcctgattt
     1141 aaatttaatc cactatggta atttaaggca tgtttgcacc tttagacata aggagacctt
     1201 tgcaaaattc cccaaaatcc cctaaattcc caccaagaca tttaggagat ttctcatgag
     1261 caccttcttc cagcaaaccg cccaagccat gattgccaaa cacatcaacc gcttcccgct
     1321 attgaagttg gaccgggtaa ttgattggca gccgatcgag cagtacctga accgtcaaaa
     1381 aacccgttac cttagagacc accgcgg
//
LOCUS       DAXHTY010000174         1335 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXHTY010000174 DAXHTY010000000
VERSION     DAXHTY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.174"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..689
                     /locus_tag="ACFNMK_002012"
     CDS             <1..689
                     /locus_tag="ACFNMK_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6365190.1"
                     /translation="ADLAYAAERITHDYPQATGANNTSTVSDYFRNIRAHSIHPRVSV
                     GYDFGDWRIAADYASYRKWNNNKYSVNTKEVLRNNNAGNWQELKTENQENGSFHAVSS
                     LGLSAIYDFKLNDKFDKFKPYIGARVAYGHVKHQVHSVRKETKTTFSKPGAGATKPGK
                     IVEGPTSKPAYHESHSISSVGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTH
                     EVSLGMRYHF"
     gene            703..885
                     /locus_tag="ACFNMK_002013"
     CDS             703..885
                     /locus_tag="ACFNMK_002013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365191.1"
                     /translation="MPSEPSDGIFDAPAVDRRGAGVGKCPNRHSRQHRNLEICHSRQH
                     RNLEIRHSRAGGNPDP"
     gene            949..1143
                     /locus_tag="ACFNMK_002014"
     CDS             949..1143
                     /locus_tag="ACFNMK_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365192.1"
                     /translation="MTVQLRSDNTVISKFVIPDNSAILKPVIPDNTVISKFVIPAQAG
                     IQTCPHRNLSDKNSCPNPAF"
BASE COUNT          367 a          380 c          311 g          277 t
ORIGIN      
        1 aggcggattt agcctatgcc gccgaacgca ttacccacga ttatccgcaa gcaaccggtg
       61 caaacaacac aagcacagta agcgattatt tcagaaacat ccgtgcgcat tccatccacc
      121 cccgggtgtc ggtcggctac gacttcggcg actggaggat agcggcagat tatgccagtt
      181 acagaaaatg gaacaacaat aaatattccg tcaacacaaa agaggtgcta agaaacaata
      241 acgctggcaa ctggcaagaa ctgaagacgg aaaatcagga aaacggcagc ttccacgccg
      301 tctcttctct cggcttatcc gccatttacg atttcaaact caacgataaa ttcgataaat
      361 tcaaacccta tatcggtgcg cgcgtcgcct acggacacgt taaacatcag gttcattcag
      421 tgagaaaaga aaccaagact actttcagta aaccaggggc aggtgctaca aagccaggca
      481 aaatcgtaga aggtccgacc agcaaacctg cctatcacga aagccacagc atcagcagcg
      541 tgggtcttgg tgtcatcgcc ggtgtcggtt tcgacatcac gcccaagctg accttggaca
      601 ccggataccg ttaccacaac tggggacgct tggaaaacac ccgcttcaaa acccacgaag
      661 tctcattggg catgcgctac cacttctgat tccccgatac cgatgccgtc tgaaccttca
      721 gacggcattt ttgatgcacc tgccgttgac aggcgcgggg cgggcgtagg gaaatgcccg
      781 aaccgtcatt cccgacaaca ccgtaatctc gaaatttgtc attcccgaca acaccgcaat
      841 ctcgaaattc gtcattcccg cgcaggcggg aatccagacc cctgacgcgg cgggaatcta
      901 tcggaaatga ctgaaacccc gagattctag attcccactt ccgtgggaat gacggttcag
      961 ttgcgttccg acaacaccgt aatctcgaaa ttcgtcattc ccgataacag cgcaatcttg
     1021 aaacccgtca ttcccgacaa caccgtaatc tcgaaattcg tcattcccgc gcaggcggga
     1081 atccagacct gtccgcacag aaacttatcg gataaaaaca gttgcccaaa ccccgcgttc
     1141 tagattccca ctttcgtggg aatgacggga tgcaggtttc cgtatggatg gattcgtcat
     1201 tcccgcacag gcgggaatcc agacctccga cgcggcggga atctatcgga aatgactgaa
     1261 accccgagat tctagattcc cactttcgtg ggaatgacgt ggtgcaggtt tccgtatgga
     1321 tggattcgtc attcc
//
LOCUS       DAXHTY010000175         1305 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXHTY010000175 DAXHTY010000000
VERSION     DAXHTY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.175"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..224)
                     /locus_tag="ACFNMK_002015"
     CDS             complement(<1..224)
                     /locus_tag="ACFNMK_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFC6365193.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVN"
     gene            complement(372..524)
                     /locus_tag="ACFNMK_002016"
                     /pseudo
     CDS             complement(372..524)
                     /locus_tag="ACFNMK_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
BASE COUNT          405 a          275 c          254 g          371 t
ORIGIN      
        1 taatttaccc gagtacacgg tgtttcatct tcactcagga cataattgtt cttctccaat
       61 ggttgctcga aacggacatt tgcatcagtt aacaattcag catctatgtg ctgctgacgc
      121 tgcatggaac ggataagttc tgcatcgttt tcatcggcag ctaaggtttt aaggggtatg
      181 acagccagga taaccaacag acatggagca ggaaaaaatt tcatgacatc aatattattt
      241 tagcaatatt tactattttg tcataaattt aaaagtattt acagttatag aatgagacct
      301 ttgcaaaatt ccccaaaatt cccaccaaga catttagggg attttgggga attttgcaaa
      361 ggtctcggac agtattttga acgcagtgcg cgtaaatccg tatggaaacc atgaaatccc
      421 gccacagccg ccagacatgc caagccgcat tctgatattt ctgtttgcag gataacaggc
      481 agctttttct ttaagcccaa agacaggttt tgcagatggg gcatagattt cctttttgaa
      541 aaatagggat taggaagttg gatgtatttt agaaaggccg cctgaaaagg tttcagacga
      601 ccttttgcga ctagctgcta ttttatttaa agcttttctc taacaaacga gctaatattt
      661 ttcctgtaat aaaacagata aaaaacagca tccaatacgt cagattggaa aaatcggtcg
      721 tatagagaat caacatataa agaagcagca tgatgccgag tgcgatgaat tgataatgtt
      781 tggcaaacat catgacctcc tcaactatta aggcaaaccg cctgaatatt ctcgttcaat
      841 cgtttcggca atttccctat aacgtcgata ccatgaccag tcgaaatttt caatggcatg
      901 gctcgcaaac gtaccaaatt caggcatccc tatgcggcta cctgctaaag ctccgattgt
      961 agctccccaa ccaggcggat tactgaacgt attgtttggc aatcctaaag atttatcagg
     1021 atttcccgta tcgttaagcc ctgcagattc gcaaatttcg caataatatg agctttgtta
     1081 cgcattacct gagcctccga cccaagtcat ttcatgaaca catatagtgg attaaattta
     1141 aatcaggaca aggcgacgaa gccgcagaca gtacaaatgg tacggcaagg cgaggcaacg
     1201 ctgtactggt ttaaatttaa tccactataa aactctcatt ttgaaactcc ttgtatcgtt
     1261 aatcaaacaa tcaaaagggc agatgcccta tccttgcttt tacaa
//
LOCUS       DAXHTY010000176         1126 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXHTY010000176 DAXHTY010000000
VERSION     DAXHTY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.176"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            81..1046
                     /locus_tag="ACFNMK_002017"
     CDS             81..1046
                     /locus_tag="ACFNMK_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6365194.1"
                     /translation="MLRLTALAVCTALALGACSPQNSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYEALNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
BASE COUNT          286 a          322 c          295 g          223 t
ORIGIN      
        1 ttatcattta tcctttctaa agccgttccg gtttgtccga ccggcggctt tgccccaata
       61 tccccatttt ggagacacct atgttacgtt tgactgcttt agccgtatgc accgccctcg
      121 ctttgggcgc gtgttcgccg caaaattccg actctgcccc acaagccaaa gaacaggcgg
      181 tttccgccgc acaatccgaa agtgcgtccg ttaccgtcaa aaccgcgcgc ggcgatgttc
      241 aaataccgca aaaccccgaa cgtatcgccg tttacgattt gggtatgctc gacaccttga
      301 gcaaactggg cgtgaaaacc ggtttgtccg tcgataaaaa ccgcctgccg tatttagagg
      361 aatatttcaa aacgaccaaa cccgccggaa ctttgttcga gccggattac gaagccttaa
      421 acgcctacaa gccgcagctc atcatcatcg gcagccgcgc agccaaggcg tttgacaaat
      481 taaacgaaat cgcgccgacc atcgaaatga ccgccgatac cgccaacctc aaagaaagtg
      541 ccaaagagcg catcgacgcg ctggcgcaaa tcttcggcaa gcaggcggaa gcggacaagc
      601 tgaaggcgga aatcgacgcg tcttttgaag ccgcgaaaac tgccgcacaa ggcaaaggca
      661 agggtttggt gattttggtc aacggcggca agatgtccgc cttcggcccg tcttcacgac
      721 tgggcggctg gctgcacaaa gacatcggtt tgcccgctgt tgacgaagcc atcaaagaag
      781 gcagccacgg tcagcctatc agctttgaat acttgaaaga gaaaaatccc gactggctgt
      841 tcgtccttga ccgcagcgcg gccatcggcg aagagggtca ggcggcgaaa gacgtgttga
      901 ataatccgct ggttgccgaa acaaccgcct ggaaaaaagg acaagtcgtt tacctcgttc
      961 ccgaaactta tctggcagcc ggcggcgcgc aagagctgct gaacgcgagc aaacaggttg
     1021 cggatgcttt taacgccgcg aaataatgaa acggcggcat tcgatgccgt ctgaaacacg
     1081 gatgcaaacc gcttcccgtg tttcagacgg cattgcccga tacggg
//
LOCUS       DAXHTY010000177         1108 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXHTY010000177 DAXHTY010000000
VERSION     DAXHTY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.177"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(166..552)
                     /locus_tag="ACFNMK_002018"
     CDS             complement(166..552)
                     /locus_tag="ACFNMK_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365195.1"
                     /translation="MWKIYKENSTDLNFAIGSIYCQAINLTEFKMWVEKIIREVDLDE
                     IPNYFFDLIDFQSLYDLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPISKEKA
                     LKALKKHPEIYQRFQHFFPFVELPPL"
     gene            complement(555..800)
                     /locus_tag="ACFNMK_002019"
     CDS             complement(555..800)
                     /locus_tag="ACFNMK_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6365196.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLVPEWEHSKTGHIGGTAMDKGK"
     gene            complement(878..>1108)
                     /gene="imm31"
                     /locus_tag="ACFNMK_002020"
     CDS             complement(878..>1108)
                     /gene="imm31"
                     /locus_tag="ACFNMK_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC6365197.1"
                     /translation="KLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDGIIYG
                     YTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
BASE COUNT          331 a          200 c          187 g          390 t
ORIGIN      
        1 caggcggtta ggacgagggg gatgaggagg agcagggttt tcatggcagg gtttgttttg
       61 ttttgaacga gtttgagtgt aaatggattt taagggtttg taaacgaaag aggcaaaaat
      121 gccgtctgag cggcggaaat ggctttcaga cggcattttg actgtttaaa gcgggggcag
      181 ttctacaaac ggaaagaaat gctgaaacct ctgataaatt tcaggatgtt tctttaaggc
      241 ttttaatgct ttttcttttg aaatgggagg atcgtagaca tctatccccc ttaaaaaagc
      301 aataccggtt aatgcattat cctgattttt tgataagtta ttttcgggaa caaatcctat
      361 aatatcaatc agatcgtata gtgattgaaa atctatcaaa tcaaaaaaat aatttggaat
      421 ttcatctaaa tccacttccc ttatgatttt ttcgacccac attttaaatt cggtaagatt
      481 gattgcttgg caatatatac tgcctatggc aaaatttaag tcggtgctat tttctttata
      541 aattttccac ataatcattt acctttatcc attgccgtcc ctcctatgtg accggtttta
      601 gaatgttccc attcaggtac aagctgcata cgtccagtat cctgatgatg atgccaagta
      661 taatccggta ttttagattc tcctttttcg attgccttta gttgctttgt tgtaaaagca
      721 gaactacgta cttctcctcg tttaatggct tccgccaaat ccttggttgc cagcctcatt
      781 tgccctgtag aatccttcaa tctgaactcc ttatgcttac ataaacataa ttaccagttt
      841 cagtatctaa aaaacttgct taaattactt aaattagcta ataaaaatca tcgcgagaga
      901 atttttttcc agtcggtata atatatttct tatctatata tatacaatat tttatatcaa
      961 aaagtaaaat agtatagcca tagattatac catcttcttc gcttatacct aataccactc
     1021 ctttttttcc tttaatagtc tcaccatttt ctaacaaatc attagggcaa gaatctttta
     1081 taataacttc agagtagaaa tctaattt
//
LOCUS       DAXHTY010000178         1084 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXHTY010000178 DAXHTY010000000
VERSION     DAXHTY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.178"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(756..>1084)
                     /locus_tag="ACFNMK_002021"
     CDS             complement(756..>1084)
                     /locus_tag="ACFNMK_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HFC6365198.1"
                     /translation="KAMRFFLDRFAALPSERLKAVDWMLFSSFLDKGGMTVCQLTPAL
                     CVQSETLPSQLKNGRQESYRNRRSPKVLLKRALGKIGREIERARERKRQKKLEKHLGR
                     HVVPFE"
BASE COUNT          266 a          252 c          262 g          304 t
ORIGIN      
        1 attttaggtt tctgtttttg gttttctgtt ctcgcgggaa taacggaact ttaagtttta
       61 ggaatttgtc ggaaaaacag aaatcccccc cgccgtcatt cccgcaaaag cgggaatcta
      121 gaaatttaac gttgcggtga tttatcggaa atgactgaaa ctcaacggac tggattcccg
      181 cctgcgcggg aatgacgaga ttttaggttt ctgtttttgg ttttctgttc tcgtgggaat
      241 aacggaattt taagttttag gaatttgtcg gaaaaacaga aatccccccg ccgttattct
      301 cgcaaaagcg ggaatctgga aatttaatgt tgcggtgatt tatcggaaat gactgaacct
      361 caacggactg gattcccgcc tgcgcgggaa tgacgaattt taggtttctg ttttcaggtt
      421 tctgtttttg gttttctgtt ctcgcgggaa taacggaatt ttaagtttta ggaatttgtc
      481 ggaaaaacag aaatcccccc ccgccgtcat tcccgcgcag gcaggaatct agaattttaa
      541 tgcggcaaga atttatcgga aaaaaccgaa gtttaaagac ctagattccc gcctgcgcga
      601 gaatgacgag attttaggtt tctgtttttg gctttctgtt ttctcgggaa tcacgaatta
      661 gggcttaccc gaaaacaagt gaaaccgaac aaaccggatt ccccgacata aatttaggga
      721 cagcgcacac ccgccaaata aggaaaggcc aaaacctatt caaacgggac gacgtgccgc
      781 ccgaggtgtt tttccaattt tttctgacgt ttccgttccc tcgccctttc gatttccctg
      841 ccgatttttc ccaaagcgcg tttcaacaaa accttcgggg agcggcggtt gcggtacgat
      901 tcctgcctgc cgtttttcag ttggctgggc agggtttcgc tttggacgca caaggcgggg
      961 gtcagttggc aaaccgtcat cccccctttg tcgagaaaac tgctgaagag catccaatcc
     1021 accgccttca ggcgttcaga cggcagggcg gcaaacctgt ccaggaaaaa ccgcatcgct
     1081 tttc
//
LOCUS       DAXHTY010000179         1075 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXHTY010000179 DAXHTY010000000
VERSION     DAXHTY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.179"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..683
                     /locus_tag="ACFNMK_002022"
     CDS             <1..683
                     /locus_tag="ACFNMK_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC6365199.1"
                     /translation="FTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRV
                     YEKGKQLGDKTSTWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFVQKATRIH
                     AVKEDKVISADRYLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLAPE
                     AYDCAFLKAQAIHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKF
                     ARLPISWA"
     gene            728..1018
                     /locus_tag="ACFNMK_002023"
     CDS             728..1018
                     /locus_tag="ACFNMK_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365200.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGRSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
BASE COUNT          347 a          254 c          231 g          243 t
ORIGIN      
        1 tgtttacctg tcatcacgtc aaaccaaaag gcgaatgttt ggggtcagat tgggaagaag
       61 acgatgaagc caaaatgacc aaaggcaaga cctatggtat cggctcccgt gaatcgtcca
      121 aatatgtccg cgtctatgaa aaaggcaagc agttgggcga taaaacaagc acatggacgc
      181 gctttgaaat tgaattcaaa gcaaaagaca tcgttatccc ttttgaagtt ttgcagaatc
      241 cgggcgaata tttcggcggc gcatatccga tttgcgaacg attcgtccaa aaggcaacgc
      301 gcatacacgc ggttaaggaa gataaggtca tttcagccga ccgctacctt gaatgggtaa
      361 aaaaacagtt tggacgtgcg gcaaacggtc tgaaattcat ttttcccgaa ttggacaaag
      421 ccaaactgtt tgaactgatt gagccgagtc atcacaagct gcccaagtct ttggctcccg
      481 aagcctacga ctgcgccttt ttgaaagctc aagccattca tgaacagccc gcattcaaac
      541 cgtacaaaga cccttactat atgtacgaat attacgagaa tcttgaaaaa cagcttgaac
      601 agcaaaaaca cgtcaacaat gaagaaagct ataacaactt catttacgac aaattcgcaa
      661 gactaccgat ttcatgggct taaagtgtct gtccgaaaga cgtttaatca cacaaggaaa
      721 ccaaaaaatg aacatccaac ttcaaggcca catcgtcggc gttaaaaaat tcaacggaca
      781 aatcgaaggc aggagcttcg actattgccg cctgattgtc gccacaccct tagacagctc
      841 ccaaggcaac gcattgggca gctctactac tgaatacgat ttcggcggct ctgccaattt
      901 cgagcagttc cgaaacgccc aatttcccat cgaagcaaac ctgaacgtgg aaatagttac
      961 tacgggcaaa acccaaaaac tgaaagtcat cggttttcaa ctcgtgaaga aaggctgatt
     1021 gaatgcagaa agtctatgtt gtccagtccg tatcaacagg ggactttctg tatct
//
LOCUS       DAXHTY010000180         1074 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXHTY010000180 DAXHTY010000000
VERSION     DAXHTY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.180"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(123..551)
                     /locus_tag="ACFNMK_002024"
     CDS             complement(123..551)
                     /locus_tag="ACFNMK_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFC6365201.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(532..966)
                     /locus_tag="ACFNMK_002025"
     CDS             complement(532..966)
                     /locus_tag="ACFNMK_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFC6365202.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          280 a          320 c          195 g          279 t
ORIGIN      
        1 atttgaaaac cccattaaac cgtctttaac ccatcgtccc gaacggtaga tgtttcaccc
       61 ctgcaaacca aaaaaaagac cgtctgaata cagacggcca aagcctggtc acacatcact
      121 gcctaaaata aagctgcctg ctgtgccgca ggtcggctca tttcattgat aatcgtatat
      181 cccgttcgtg aagagatgcc gtatttaggg catagcttcg tcatcgccat aagcccgctc
      241 tccttatcaa tatcgcgcaa tttgacaaac tcctgataaa acctatggtt tctcaactgt
      301 attaacgcct tgccgcaccg tgggacatac aattcctcgc caccatatac ctgcaacagc
      361 tcatgtgttt tcacttcgcc gatggcttcg accaaaattg ccaaacgctc ggtgtccacc
      421 ttgcccttac caaatttaaa ccgcgccccg ccaatcgcct tgaccagctg ttccgtcgct
      481 gccagtccga tgacatccac aatgtccaac acggtatccg gcaataaatg ttcaactttt
      541 tcgaacccca tcatccccac ccgctttttc cgttttcctg ttttccgcaa tctgcaacgc
      601 agcaaccagt ttatgtagct gcgtatcgtc taaatattcg accttatcct taccaaacat
      661 ccgccgcgcc attgcgtgtg catagttcca atgtttgccg ccgacggtca gcagggcttc
      721 gactttgtcc aacattgccg ctgatgatgt ccgacgcaga tgtggtttac cgtgtgggtt
      781 accttttgct ttaggcttaa atccgtgcga ccgcatatca gcgacaacag actcaagttc
      841 ggaaacatcc atatccgcac acgaccgctt gcccgtcacg cgctccaaca ccgcgcgata
      901 ggtaccgtcg tccaagccca gctccttttg ggcaatttta atttttgcaa tcaacgcccg
      961 gcgcatctca aacccataaa acacaatata ttgactaatt aacgcatatt ataccaataa
     1021 aatacaatat atagtattaa gccgatgttt tttgcgaaac ggacagacat gaaa
//
LOCUS       DAXHTY010000181         1035 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXHTY010000181 DAXHTY010000000
VERSION     DAXHTY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.181"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..>1035
                     /gene="tuf"
                     /locus_tag="ACFNMK_002026"
     CDS             <1..>1035
                     /gene="tuf"
                     /locus_tag="ACFNMK_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6365203.1"
                     /translation="RSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQID
                     NAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAA
                     DGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDD
                     CPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRG
                     TVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTK
                     REDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGA
                     VTLEEGVEMV"
BASE COUNT          276 a          293 c          263 g          203 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggtggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgcccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatt gtacaaggtt ctgcactgaa agccttggaa ggcgatgccg cttatgaaga
      541 gaaaatcttc gaactggctg ccgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaaccg ttcctgctgc ctatcgaaga cgtgttctcc atttccggcc gaggtacagt
      661 agtaaccggc cgtgtagagc gcggtatcat ccacgttggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa acccaaaaaa ccacctgtac cggtgttgaa atgttccgca aactgctgga
      781 cgaaggtcag gcgggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 ggaacgcggt caggtattgg ccaaaccggg tactatcact cctcacacca aattcaaagc
      901 agaagtgtac gtactgagca aagaagaggg cggccgccat actccgttct tcgccaacta
      961 ccgtccgcaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
     1021 tgtggaaatg gtaat
//
LOCUS       DAXHTY010000182          984 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXHTY010000182 DAXHTY010000000
VERSION     DAXHTY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.182"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
BASE COUNT          223 a          208 c          288 g          265 t
ORIGIN      
        1 ttacaaccgt aaaaaagtaa gtgccgccat tgcggtaaaa acgacggtat ttcatagtat
       61 tatgctcgga atgattttgt aggtcggatt cttgaattcg acattttggg cattgctgca
      121 atggattgca atgatgggaa tgttaaaggt tttgtcggat acaagtatcc gacctacgct
      181 tgctgaaccg tcattcccac ggaagtggga atctagaacg cagggtttgg gcaactgttt
      241 ttatccgata agtttctgtg cggacaggtc tggattcccg cctgcgcggg aatgacgaat
      301 ttcgagattg cggtgttgtc ggaacgcaac tgaaccgtca ttcccacgga agtgggaatc
      361 tagaatctcg gggtttcagt catttccgat agattcccgc cgcgtcgtgg gtctggattc
      421 ccgcctgcgc gggaatgacg aatttcaaga ttgcggcgtt gtcggaaatg acgggtttca
      481 agattacggt gttgtcggaa cgcaactgaa ccgtcattcc cacgaaagtg ggaatctaga
      541 atctcggggt ttcagtcatt tccgatagat tcccgccgcg tcagggggct agattcccgc
      601 ctgcgcggga atgacgaatt tcgagattac ggtgttgtcg ggaatgacgg gtttcaagat
      661 tgcgctgtta tcgggaatga cgggtttcga gattacggtg ttgtcggaac gcaactgaac
      721 cgtcattccc acggaagtgg gaatctagaa tctcggggtt tcagtcattt ccgatagatt
      781 cccgccgcgt cgggggtctg gattcccgcc tgcgcgggaa tgacgaattt cgagattacg
      841 gtgttgtcgg aacgaaactg aatcgtcatt cccacgaaag tgggaatcta gaacgcgggg
      901 tttgggcaac tgtttttatc cgataagttt ctgtgcggac aggtctagat tcccgcctgc
      961 gcgggaatga cgggttttaa gatt
//
LOCUS       DAXHTY010000183          897 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXHTY010000183 DAXHTY010000000
VERSION     DAXHTY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.183"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(38..604)
                     /locus_tag="ACFNMK_002027"
     CDS             complement(38..604)
                     /locus_tag="ACFNMK_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HFC6365204.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAVIGMATTVMVTVGLTAGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          243 a          286 c          193 g          175 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactatattt tccgacatca ttgaatcaaa cccaaatgcg
       61 acaagagcgt ccatgtaccg atggcaatca acaccaaacc tccggcaaat tccgcacgcc
      121 tgccgaacaa tacgcccaaa gcccttcccg ccgtcagccc gaccgtaacc atcaccgtcg
      181 tcgccatgcc gatgacggcg gcggcaaagg cgatgtttac ctccataaac gccaagccca
      241 ccccgactat catggaatca atactggttc caaaagcagt cagaaccgtc aaccaccagc
      301 tttcccgttt gctttcgcgt acatcttccg ccttgccgga cagcccttcg cgcatcattt
      361 tcagacccag cccgcccagc aggacgaaag ccgcccaatg gtcccattcg ctgataaacg
      421 gtttggcata aaaaccgcct acccagcctg ccagcggcgt gagtgcctca accgtgccga
      481 acaccaaagc cgttgctgca attttgcgcg ggggcatcct gaccgccgca ccctttgcca
      541 atgcgacggc aaacgcatcc atcgacatcc ccagcgcaat caagagcaaa gcgtaaagac
      601 tcataccgca cccatcctca aaaagggcag attatagcaa aacagcaaag cgcgaaaaac
      661 gccgcacgaa aacccacatc ccgtcattcc tgcaaaaaca aaaaatcaaa aacagaaatc
      721 ccgtcattcc cgcgcaggcg ggaatccaga ccggtcggtg cagaaactta ttggataaaa
      781 cggtttcttt agattttacg ttctagattc ccactttcgt gggaatgacg ggatgtaggt
      841 ttccgtgcgg atggattcgt tattcccgcg caggcgggaa tccaaagttg tcggtgc
//
LOCUS       DAXHTY010000184          759 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXHTY010000184 DAXHTY010000000
VERSION     DAXHTY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.184"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..>759
                     /gene="pilC"
                     /locus_tag="ACFNMK_002028"
     CDS             <1..>759
                     /gene="pilC"
                     /locus_tag="ACFNMK_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6365205.1"
                     /translation="RVVIFGTGSDLSEEDVDKKDEQYIYGIFDDDTATSNVDVKLKGL
                     GGGLLEQVLEQKDKTLFLTDYKRSDGSGSKGWVVKLKDGQRVTVKPTVVLRTAFVTIR
                     KYTDNGCGAETAILGINTADGGKLTKKSARPIVPDANKDVAQYSGHKKGINGKSIPIG
                     CMWKNNETVCPNGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNR
                     CFSQKGVRTLLMNDLDSLDITGPMCGMKRISWREV"
BASE COUNT          215 a          175 c          219 g          150 t
ORIGIN      
        1 cgcgtggtta tcttcggcac gggcagtgat ttgagtgaag aggatgtgga caaaaaggac
       61 gaacaatata tttacggtat ctttgacgac gatacggcga cgagtaatgt agatgtaaaa
      121 ctcaaaggtt tgggaggcgg gttgctcgag caggtgcttg agcagaagga taaaacctta
      181 ttcctgaccg attataagcg atccgacggc tcgggcagca aagggtgggt agtgaaattg
      241 aaggacggac agcgcgttac cgtcaaaccg accgtggtat tgcgtaccgc ctttgtaacc
      301 atccgcaaat atactgacaa cggctgcggc gcggaaaccg ccattttggg catcaatacc
      361 gccgacggcg gcaagctgac caagaaaagc gcgcgcccga ttgtgccgga cgccaataag
      421 gatgtcgcgc aatattccgg tcataagaaa ggcatcaacg gcaaatccat ccctataggt
      481 tgtatgtgga aaaacaatga aaccgtctgc ccgaacggat atgtttacga caaaccggtt
      541 aatgtgcgtt atctggatga aaagaaaaca gacggatttt caacaacggc agacggcgat
      601 gcgggcggca gcggtataga ccccgccggc aagcgttccg gcaaaaacaa ccgctgcttc
      661 tcccaaaaag gggtgcgcac cctgctgatg aacgatttgg acagcttgga tattaccggc
      721 ccgatgtgcg gtatgaaacg aatcagctgg cgtgaagtc
//
LOCUS       DAXHTY010000185          734 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXHTY010000185 DAXHTY010000000
VERSION     DAXHTY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.185"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..181
                     /locus_tag="ACFNMK_002029"
     CDS             <1..181
                     /locus_tag="ACFNMK_002029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365206.1"
                     /translation="PYWFLLIHYKSKETAISTTPPQAAAAKHIGCFSTHPLKNASYPS
                     ALPAPPDFAALPDAV"
     gene            complement(124..675)
                     /locus_tag="ACFNMK_002030"
     CDS             complement(124..675)
                     /locus_tag="ACFNMK_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6365207.1"
                     /translation="MKVLSGWSGRKMWRVLSALPIGVVFFDLIYGFALNVLQGLDLQR
                     AVPDSESVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          204 a          283 c          140 g          107 t
ORIGIN      
        1 accgtactgg tttttgttaa tccactataa aagcaaagaa accgccatta gcactacacc
       61 cccccaagct gcggctgcca aacacatcgg ctgcttctcg acgcacccgc tgaaaaatgc
      121 gtcctatccg tccgccttgc ccgcaccccc tgactttgcc gccctgcccg atgccgtctg
      181 aagcctgaac cgccccacca gcccgatgac gcacgacacc gccggaaagg gcatacacaa
      241 agccgtcagc acataacgcg cattccccgt attgaccacg tccgcgccct tcagcagcag
      301 cggcagcgcg ttcgcccact cccacaccga cggcgcgctg aacgccaata ccgccaccag
      361 ccccaacatc cggaacaccc cgatttccaa cacctgccgc cgccgcaccg aacggttgag
      421 caaaaacaca accgccaacc cgaaacagac caccgccgcc ataccgccgt tggcgacaat
      481 ctgcaaactg ttgaatgcaa tatcgggcgt aaccgccaac acgctttccg aatccggcac
      541 ggcacgctgc aaatccaaac cctgcaacac attcaacgca aaaccgtaga tcaaatcaaa
      601 aaacaccacg cctatcggca aagcactcaa aacccgccac atcttcctgc ccgaccaacc
      661 gctcaaaact ttcattttac ccgaccacgc aagccgccga acaaaaaaca aggggctgtc
      721 ctagataact agga
//
LOCUS       DAXHTY010000186          722 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXHTY010000186 DAXHTY010000000
VERSION     DAXHTY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.186"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(125..>722)
                     /locus_tag="ACFNMK_002031"
     CDS             complement(125..>722)
                     /locus_tag="ACFNMK_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6365208.1"
                     /translation="LSIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPV
                     QSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGV
                     DIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQV
                     KRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          160 a          208 c          197 g          157 t
ORIGIN      
        1 acacagtcaa acaaatttat atgccccatc ccttccgaat aatttgaaaa cacagccgcc
       61 aaaaacaaaa atgccgtctg aaaacctttc agacggcatt tccaacttga tttcaggcag
      121 aaagtcagaa cgcgatatag ctgttcggat taaccggttt gccgttttgc cgcacctcga
      181 aatgaagctg cgttctggaa gcgtcggtat tgcccatcaa agcgacctgc tgcccgcgtt
      241 tgacctgctg gccttcgccg accagcaatt tttggttgtg cccgtatgcg gtcaggaagg
      301 aagaattatg ctggatgatg accagattgc cgtatcccct caaaccggaa cctgcataaa
      361 ccactttgcc gtcagccgcc gccaaaacgg gctgtcccgc attaccggca atatcgacac
      421 ccttgttgtt gccgccgaaa tcggcaacca ctttaccttg cgtcggacgc tgccaaacaa
      481 tgccgccgac cgaacgcgtg ccggaaggcg aagcggcagg agattgcggg gcgggcgcgg
      541 gaaccgcttt attttccgca gcgggcgcgg caggttgcgg cgcggactgc acaggcggtt
      601 gcgcggcggg tttcacaggg gtttgcacgg cagccggtac ggcgggcctg ctttctacgg
      661 ctgcggtttt cggtgcggca tatcctgccg gtttgacttt aacaatctga ccgatgctca
      721 ac
//
LOCUS       DAXHTY010000187          699 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXHTY010000187 DAXHTY010000000
VERSION     DAXHTY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.187"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(69..554)
                     /locus_tag="ACFNMK_002032"
     CDS             complement(69..554)
                     /locus_tag="ACFNMK_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC6365209.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          210 a          217 c          127 g          145 t
ORIGIN      
        1 tgttaatcca ctatattttc aaaccggaaa aatgccgtct gaaccttcag acggcattcc
       61 attcacattt attcgtcttt tttgtgttcg atggcgtttc gggaacgatt gccgccgtaa
      121 ggctcttcaa ccgtatattc cccctcgata atatcgtcat cgcgggaaaa gccctctttt
      181 ctgcccgatt ggttcatgtt gaaaaaattt tccgcacctc ctgcctgcaa cactgcacct
      241 cccttaaacg gcagcagcag caataccgcc aacaccgagg atacgaatcc cggactcatc
      301 agacacaccg ccgccaccgt ataacggata ggccacaaca tctgataaac ggataccctc
      361 ccgccgcttc tcattgccgc gcccgccaat aaaagaccgg acagccccgt atgcctgagc
      421 atcagcacgc cggcggcaaa ggttgccgcc attagaaaca gcgtccaacc gccgcccaac
      481 caatcggcaa cccacacaat cgacataatc tccaaaaaca gcagcaccaa aaaaccgata
      541 ccgaaaaatc tcattgaccg tcatccttat atttaagtaa acagcaaacc gcccgaacag
      601 gactccaagc gagctgcctg taaatgatta caaaaccatg tgcttcaagc cgaaacaatg
      661 tgaaatcacg caatatagtg gattaacaaa aaccagtac
//
LOCUS       DAXHTY010000188          678 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXHTY010000188 DAXHTY010000000
VERSION     DAXHTY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.188"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..121)
                     /locus_tag="ACFNMK_002033"
     CDS             complement(<1..121)
                     /locus_tag="ACFNMK_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6365210.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNR"
     gene            complement(242..553)
                     /gene="rpsJ"
                     /locus_tag="ACFNMK_002034"
     CDS             complement(242..553)
                     /gene="rpsJ"
                     /locus_tag="ACFNMK_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC6365211.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(571..>678)
                     /locus_tag="ACFNMK_002035"
     CDS             complement(571..>678)
                     /locus_tag="ACFNMK_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6365212.1"
                     /translation="TVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
BASE COUNT          170 a          147 c          171 g          190 t
ORIGIN      
        1 gacggttcag gtattgttcg atcggttgcc aatcaatcac ttgatctagc ttcaatagtg
       61 ggaagcggtc gatgtgtttg gcaatcatgg cttgtgcggt ttgctgaaag aaggtgctca
      121 tggaaaatcc cctaaatgtc ttggtgggaa tttaggggat tttggggaat tttgcaaagg
      181 tctcggcata acataaaaaa tccccaagca ttgattgctt ggggattttc aatagtccga
      241 attattggac tttgatttct acatcaacac cggccggcaa atccagcttc atcagcgcat
      301 cggtagtttt atcggtccaa tccacgatgt ccatcaggcg caagtgggtg cggatttcca
      361 attgttcacg ggaagttttg ttcacgtgcg gagaacgcag aatgttgaaa cgttcgattt
      421 tggtcggcaa cggaatcgga ccttttacaa cggcaccggt acgttttgca gtttcaacga
      481 tttcttgtgc agaacggtca atcagggcgt aatcataagc tttcaggcgg atacggattt
      541 tttggtttgc catttatcaa tatccttcaa ttaagcgata acagaagaaa ccacgccggc
      601 acccacggta cgaccgcctt cgcgaatcgc aaagcgcagg ccttcttcca tagcgatagg
      661 cgcaatcagt tctacggt
//
LOCUS       DAXHTY010000189          657 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXHTY010000189 DAXHTY010000000
VERSION     DAXHTY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.189"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            498..>657
                     /locus_tag="ACFNMK_002036"
     CDS             498..>657
                     /locus_tag="ACFNMK_002036"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6365213.1"
                     /translation="MPACAGMTMQVFLTIPGYDVEAEIEKFVWMDAVIWQMPGWWMHE
                     PWTVKKYID"
BASE COUNT          179 a          152 c          163 g          163 t
ORIGIN      
        1 aattaggcgg tatttggggc gaaaacaact gaaaacctgt gtttgggttt cggctgtcgg
       61 gagggaaagg aattttgcaa aggtctcttt tcgtcattcc cgccactttt cgtcattccc
      121 gcgaaagcgg gaatctagaa tctcggactt tcagataatc tttgaatatt gctgttgttc
      181 taaggtctag attcccgcct gcgcgggaat gacaactcat tagttaccta aaacttaaaa
      241 agcagaaacc tttacgccgt cattcccacg aaagtgggaa tccgggaact taacgttaca
      301 gtgatttatc ggaaacggct gaaaccgaat gaattggatt cccgcctgcg cgggaatgac
      361 gaatccatcc gtacggaaac ctgcaccacg tcattcccac gaacctacat tccgtcattc
      421 ccacgaaagt gggaatccag gacgaaaaat ctccagaaac cgttttaccc gataagtttc
      481 cgcaccgaca ggtctggatt cccgcctgcg cgggaatgac gatgcaggta tttctgacaa
      541 ttcccggcta tgatgttgag gcagaaatcg aaaaattcgt ttggatggat gctgtgattt
      601 ggcagatgcc gggctggtgg atgcacgagc cttggacagt gaaaaaatac atagacg
//
LOCUS       DAXHTY010000190          646 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXHTY010000190 DAXHTY010000000
VERSION     DAXHTY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.190"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
BASE COUNT          140 a          167 c          156 g          183 t
ORIGIN      
        1 atttcaatgc ctcaagaatt tatcggaaaa aaccaaaacc cttccgccgt cattcccaca
       61 aaagtgggaa tctagaaatg aaaagcagca ggaatttatc ggaaatgacc gaaactgaac
      121 ggactggatt cccgctttcg cgggaatgac ggcgacaggg ttgctgttat agtggatgaa
      181 caaaaaccag tacgtcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctc
      241 aagcaccaag tgaatcggtt ccgcactatc cgtactgtct gcggcttcgc cgccttgtcc
      301 tgatttccgt tcgttttcgg ttattcccga taaattaccg ccgtttctcg tcatttcttt
      361 aacccttcgt cattcccgcg caggcgggaa tctagttttt taagttccag ttgtttctga
      421 taaattcttg cagctttgag ttcctagatt cccgctttcg cgggaaggac gcggaaaggt
      481 tgcttttcgt cattcccgcg caggcctcgt cattcccgcg caggcgggaa tccagtctgt
      541 tcggtttcgg ttttttccga tagattcctg ccgcgttagg ggttctagat tcccgctttc
      601 gcgggaagga cgcggaaagg ttgcttttcg tcattcccgc gcaggc
//
LOCUS       DAXHTY010000191          631 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXHTY010000191 DAXHTY010000000
VERSION     DAXHTY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.191"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..374
                     /locus_tag="ACFNMK_002037"
     CDS             <1..374
                     /locus_tag="ACFNMK_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HFC6365214.1"
                     /translation="TAGYIISKKAIRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVP
                     VCQLNPALCAQELHYAKFHDQNSALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALT
                     KISREREKRRQRREQFIVPFQ"
     gene            392..481
                     /locus_tag="ACFNMK_002038"
                     /pseudo
     CDS             392..481
                     /locus_tag="ACFNMK_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
     gene            523..>631
                     /locus_tag="ACFNMK_002039"
     CDS             523..>631
                     /locus_tag="ACFNMK_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6365215.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALM"
BASE COUNT          165 a          179 c          158 g          129 t
ORIGIN      
        1 ggacggcggg ctatatcatt tccaaaaaag cgatacggtt tttcctggac aggtttgccg
       61 ccctgccgcc cgaagggctg caccccgtcg atctgatgat gttcagcgat tttttcgaca
      121 gggaaggagt gccggtttgc cagctcaatc ccgccttgtg cgcccaagag ctgcattatg
      181 ccaagtttca cgaccaaaac agcgcattgg gcagcctgat cgaacacgac cgcctcctga
      241 accgcaaaca gcaatggcgc gattcccccg ccaacacatt caaacgccgc ctgatccgcg
      301 ccttgaccaa aatcagcagg gaaagggaaa aacgccggca aaggcgcgaa cagttcattg
      361 tgcctttcca ataaaaggag gagagatgga catcgtattt gcagcagacg gcaggatgcg
      421 gcggctgttt accttgaggc aatacttcgg cattttgcac cgattgctga aaaaccgttg
      481 aaaaacgccg ctttatccaa cagacaaaaa acaggataaa ttatgcaaaa ccacgttatc
      541 agcttagctt ccgccgcaga acgcagggcg cacattaccg ataccttcgg cgtgcgcggc
      601 atcccgtttc agtttttcga cgcactgatg c
//
LOCUS       DAXHTY010000192          561 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXHTY010000192 DAXHTY010000000
VERSION     DAXHTY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.192"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
BASE COUNT          148 a          133 c          146 g          134 t
ORIGIN      
        1 aaaccgaacg aactagattc ccactttcgt gggaatgacg ggatgcaggt ttccgtatgg
       61 atggattcgt cattcccgcg caggcgggaa tccagacctg tccgcacaga aacttatcgg
      121 ataaaaacag ttgctccaac cccgcgtcct agattcccac tttcgtggga atgacgggat
      181 gcaggtttcc gtacggatgg attcgtcatt cccgcgcagg cgggaatcta gacattcaat
      241 gctaaggcaa tttatcggga atgactgaaa ctcgaaaaac tggattccca ctttcgtggg
      301 aatgacggca gagcggcttc tgttgctcct gataaatgcc gcaatctcaa atcccgtcat
      361 tcccgcgcag gcgggaatct agacattcaa tgctaaggca atttatcgga aatgactgaa
      421 actcaaaaag ctggattccc actttcgtgg gaatgacggg atacaggttc gtgggaatga
      481 cgggatgcag gtttccgtat ggatggattc gtcattcccg cgcaggcgag aatctagaca
      541 ttcaatgcta aggcaattta t
//
LOCUS       DAXHTY010000193          543 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000147
            SAMN43432238-rid23052793.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXHTY010000193 DAXHTY010000000
VERSION     DAXHTY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432238
            Sequence Read Archive: SRR30498656
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346371.1
            Genome Coverage       :: 823x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 16:06:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,860
            CDSs (with protein)               :: 1,860
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 77 of 125
            Pseudo Genes (internal stop)      :: 23 of 125
            Pseudo Genes (multiple problems)  :: 20 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432238-rid23052793.denovo.193"
                     /isolate="NM000000147"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..383
                     /locus_tag="ACFNMK_002040"
     CDS             <1..383
                     /locus_tag="ACFNMK_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6365216.1"
                     /translation="ILLAEGQKSAVTEYYLNHGIWPGDNSSAGVATSSKIKGKYVKEV
                     TVANGVVTATMLSSGVNKEIQGKKLSLWAKRQDGSVKWFCGQPVTRDAKADKKIDTNH
                     SHEPTSRHSRESGNLGLSARKLIG"
     gene            441..543
                     /locus_tag="ACFNMK_002041"
                     /pseudo
     CDS             441..543
                     /locus_tag="ACFNMK_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
BASE COUNT          159 a          143 c          131 g          110 t
ORIGIN      
        1 ctatcctttt ggctgaaggt caaaaatcag ccgtcaccga gtattacctg aatcacggca
       61 tatggcccgg cgacaacagt tctgccggcg tggcaacctc ctctaaaatc aaaggaaaat
      121 atgttaagga agttacagtc gcaaacggcg tcgttaccgc cacaatgctt tcaagcggcg
      181 taaacaaaga aatccaaggc aaaaaactct ccctgtgggc caagcgtcaa gacggttcgg
      241 taaaatggtt ctgcggacag ccggttacgc gcgacgccaa agccgacaag aaaatcgaca
      301 ccaatcattc ccacgaacct acatcccgtc attcccgcga aagcgggaat ctaggtctgt
      361 cggcacggaa acttatcggg taaaaaggtt tctccggccc tgagttctag attcccactt
      421 tcgtgggaat gacgggattt aatgatgccg ccggcgtggc aacctccacc gacattaaag
      481 gcaaatatgt tgagaaagtt gaagtcaaaa acggcgtcgt taccgccgaa atggcttcaa
      541 gca
//
